vwde cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	vwde
Entrez Gene ID	100495432
Full Name	von Willebrand factor D and EGF domains
General protein information	Preferred Names von Willebrand factor D and EGF domains Names von Willebrand factor D and EGF domain-containing protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002939011.4	XP_002939057.2	von Willebrand factor D and EGF domain-containing protein isoform X1
XM_002939011.5	XP_002939057.3	von Willebrand factor D and EGF domain-containing protein isoform X1
XM_012965592.2	XP_012821046.1	von Willebrand factor D and EGF domain-containing protein isoform X2
XM_031903928.1	XP_031759788.1	von Willebrand factor D and EGF domain-containing protein isoform X4
XM_012965592.3	XP_012821046.2	von Willebrand factor D and EGF domain-containing protein isoform X2
XM_012965594.2	XP_012821048.1	von Willebrand factor D and EGF domain-containing protein isoform X3
XM_012965594.3	XP_012821048.2	von Willebrand factor D and EGF domain-containing protein isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Vwde	NP_001013779.2
Danio rerio (zebrafish)	LOC100006681	XP_001345360.4
Xenopus tropicalis (tropical clawed frog)	vwde	XP_002939057.2
Pan troglodytes (chimpanzee)	VWDE	XP_518974.3
	LOC694004	XP_002803427.1
Canis lupus familiaris (dog)	VWDE	XP_005628620.1
Bos taurus (cattle)	VWDE	XP_003585998.2
Rattus norvegicus (Norway rat)	Vwde	XP_006236185.1
Gallus gallus (chicken)	VWDE	XP_418688.4
Homo sapiens (human)	VWDE	NP_001129396.1

Gene Ontology

The following vwde gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vwde cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa60323	XM_002939011.5 Latest version!	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa60324	XM_012965592.3 Latest version!	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa60325	XM_012965594.3 Latest version!	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa60326	XM_031903928.1 Latest version!	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa12410	XM_002939011.4 Latest version!	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa36289	XM_012965592.2 Latest version!	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa36290	XM_012965594.2 Latest version!	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vwde ( XM_002939011.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939057.3 Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa60323
Clone ID Related Accession (Same CDS sequence)	XM_002939011.5
Accession Version	XM_002939011.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor D and EGF domain-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939011.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC 
CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT 
GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG 
CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA 
CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT 
TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG 
AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC 
TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC 
AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA 
GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC 
ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC 
AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA 
ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA 
CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC 
CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA 
GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA 
TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT 
CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT 
TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT 
CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA 
GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT 
GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT 
CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG 
TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC 
GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT 
AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA 
TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC 
TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT 
TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC 
AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA 
TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT 
AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA 
TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA 
ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA 
GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA 
ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT 
CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGTTAAAA ACAGATTTAA GCGGCAAGAT 
TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT 
AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG 
CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC 
AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT 
CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG 
GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA 
TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT 
GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC 
TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT 
GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA 
CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA 
GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT 
GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG 
CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT 
GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA 
ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC 
TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT 
CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT 
CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TTACCTTTGA ATCTGGTCCT 
GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG 
CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG 
GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT 
CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC 
CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA 
GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT 
TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA 
TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG 
ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT 
GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT 
TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA 
ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG 
CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT 
GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG 
ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT 
GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG 
GCAGTTACCA TGGCGTCTGC GAAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT 
ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC 
GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC 
GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT 
GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC 
GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT 
GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA 
TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT 
GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA 
GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC 
TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC 
ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT 
GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC 
CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT 
GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG 
TGCCAGACTG CTGTCTGCAA CTACAAATGT TTATATGGAG GCCGCTGTGT ACTGCCCAAT 
GTATGCTCAT GTCGCGCAGG ATACACTGGA ACAACTTGTA ACCAGAGAGT ACAGGGGTTT 
CGTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939057.3
CDS	241..5649
Translation	MSLNLQPGCATLWVLLLILQTAAAQQECSSSGHQILQNSYRSLDFDSLKLQQSAIHDVICDHSLSPGWYRFMIFDKPAEMPTKCVEMNHCGTQAPVWLSLRDSETLPPPGEIRHLTACATWQFFFSSTKDCCLFRIPVSVRNCGDFFVYLLQPTQGCMGYCAEVVPEPKSKCDGGEIDTNNECTSKVIAKPVQPEPPSNPEISIEISGDSVLLKCDFKAPITNSTMGFVIGWSRLSAEGAKEELRQEAAVQTFSIIELDGINVRLGDRIYCSVSSFFLEQPDVHSSLIESEEFFTGIKLYPESVTITEDGRQYQLTVESTVPVLCLGAIQLQSECKVTLKFHTVNEAEENRDSDLALSSCQVDLLQSPCQNHSCSQTSLYYTAVTDFMEDGDKLTNIVVDPIVSTNFLWNGYSPQSVQITVKDIPTAYCYSFTDPHVITFDGRSYDNFNTGTFVLYKSASRDFEVHVRQWDCGSLHHPASCNCGFVAKERGDIVAFDMCSGQLHESQPHLSVKSRDKTTTNFKITESYHGRKLTVVFSSGSFIRADVSDWGMSLTLRAPSSDFQNTLGLCGTFDGNADNDFHNSKGDQLEGISNNHLHFINQWKISAGGSLFDKIPQFSQLSQNTQYCNCNPDRLDAFESNKLLNPQTDMFSCHGNDNVRFHALIPGLDITSEYINALDTIRDVSKRSVADSLVYGPLPAAVEQHENIRTVSITEQTDGRIKNTISTKNNGDTKTQGNTNHINRFSPEKKAVKNRFKRQDYQYISTFPYQSLSQTDLEGFSYFFPEDHSTDVPQKFLPSWPTPSGLSHLYVSEFCHQSVFNSTIGKICNDYLKKHIKDVVQMCIVDVLLKDDLSWAEAGVALLENECERNVLELGLHREEFFKQPINDILVALTCPNHCSGNGQCMDWGCACFEGFSSYDCSILEDQVPEILELENKGLCDIRKYACTSVRAFGLGFKETPTLKCEFTKQQYSGGKWIFAEPVLTDAAFRNPRTVDCQLPVDEQQSDGMEPVDDKPIAKWQIKVSNDGYMFSNAKTMTLYDGVCQNCDALSDVLCTLKEKTCNIDGLCYGEGDPNPTSPCLLCRPDVSKLTWSIAENNQPPVIDSFPDKLQTFYGENFVYQFVGADPEGSAILFTFESGPENSSLSPAGLLIWKVTSTVTQEFVFSLTDDCNAQTQVIVEVIVKPCGCINGGSCVTNINFPPGQGEYLCLCPNGFEGDNCQVNIDECKSNPCGPGKCTDEVNGYHCECPLGLKGITCQEDLDECLSSPCYSGVFCTNTYGSYECGQCPDGYQGDGKICTQILLTTQMPLMVLTDIFTKEAGKKELYFPGGSALKSVGQSSFAVPSPTVYSRQTDSRSFQTTRSLSSSLTTSTARRTTISKLLNGRRTTSNLSRSALFASHAIKPSSRGNSTNIYPTAPTVTNSSKHHSVTSGSPAKNKTDEWPFTKHTDNYLPTLNGTDQAVTMASAKRTCADSPCYPGVICEPTISGGFKCGRCPFGYYGDGVTCKAICRHPCGKNMVCAAPNVCRCRNGYSGYNCQTAVCRPDCKNRGKCVRPNVCVCAPGYGGETCEEAYCDQPCENGGTCQARNVCTCPFGYVGPRCETMVCNRHCENGGECVAPDVCKCKPGWYGPTCSTAMCHPVCLNGGTCMKSNICLCPKGFYGAQCQNAVCSPPCKNGGQCMRNNICTCPDGYTGIRCQKSVCNPMCMNGGKCVGPNICSCPSGWKGKQCNTPICLEKCKNGGECVGPNTCHCPAEWEGSQCQTAVCNYKCLYGGRCVLPNVCSCRAGYTGTTCNQRVQGFRG

Target ORF information:

RefSeq Version	XM_002939011.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939011.5

ORF Insert Sequence:

ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC 
CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT 
GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG 
CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA 
CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT 
TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG 
AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC 
TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC 
AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA 
GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC 
ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC 
AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA 
ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA 
CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC 
CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA 
GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA 
TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT 
CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT 
TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT 
CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA 
GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT 
GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT 
CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG 
TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC 
GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT 
AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA 
TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC 
TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT 
TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC 
AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA 
TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT 
AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA 
TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA 
ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA 
GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA 
ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT 
CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGTTAAAA ACAGATTTAA GCGGCAAGAT 
TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT 
AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG 
CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC 
AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT 
CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG 
GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA 
TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT 
GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC 
TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT 
GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA 
CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA 
GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT 
GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG 
CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT 
GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA 
ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC 
TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT 
CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT 
CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TTACCTTTGA ATCTGGTCCT 
GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG 
CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG 
GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT 
CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC 
CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA 
GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT 
TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA 
TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG 
ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT 
GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT 
TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA 
ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG 
CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT 
GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG 
ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT 
GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG 
GCAGTTACCA TGGCGTCTGC GAAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT 
ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC 
GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC 
GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT 
GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC 
GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT 
GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA 
TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT 
GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA 
GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC 
TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC 
ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT 
GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC 
CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT 
GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG 
TGCCAGACTG CTGTCTGCAA CTACAAATGT TTATATGGAG GCCGCTGTGT ACTGCCCAAT 
GTATGCTCAT GTCGCGCAGG ATACACTGGA ACAACTTGTA ACCAGAGAGT ACAGGGGTTT 
CGTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwde ( XM_012965592.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821046.2 Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X2, mRNA.

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CloneID	OXa60324
Clone ID Related Accession (Same CDS sequence)	XM_012965592.3
Accession Version	XM_012965592.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor D and EGF domain-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965592.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC 
CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT 
GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG 
CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA 
CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT 
TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG 
AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC 
TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC 
AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA 
GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC 
ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC 
AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA 
ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA 
CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC 
CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA 
GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA 
TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT 
CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT 
TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT 
CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA 
GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT 
GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT 
CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG 
TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC 
GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT 
AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA 
TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC 
TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT 
TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC 
AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA 
TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT 
AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA 
TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA 
ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA 
GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA 
ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT 
CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGTTAAAA ACAGATTTAA GCGGCAAGAT 
TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT 
AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG 
CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC 
AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT 
CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG 
GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA 
TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT 
GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC 
TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT 
GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA 
CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA 
GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT 
GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG 
CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT 
GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA 
ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC 
TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT 
CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT 
CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TTACCTTTGA ATCTGGTCCT 
GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG 
CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG 
GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT 
CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC 
CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA 
GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT 
TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA 
TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG 
ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT 
GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT 
TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA 
ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG 
CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT 
GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG 
ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT 
GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG 
GCAGTTACCA TGGCGTCTGC GAAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT 
ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC 
GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC 
GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT 
GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC 
GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT 
GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA 
TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT 
GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA 
GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC 
TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC 
ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT 
GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC 
CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT 
GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG 
TGCCAGACTG GGGTTTCGTG GTTGAGAGAG GTTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821046.2
CDS	242..5560
Translation	MSLNLQPGCATLWVLLLILQTAAAQQECSSSGHQILQNSYRSLDFDSLKLQQSAIHDVICDHSLSPGWYRFMIFDKPAEMPTKCVEMNHCGTQAPVWLSLRDSETLPPPGEIRHLTACATWQFFFSSTKDCCLFRIPVSVRNCGDFFVYLLQPTQGCMGYCAEVVPEPKSKCDGGEIDTNNECTSKVIAKPVQPEPPSNPEISIEISGDSVLLKCDFKAPITNSTMGFVIGWSRLSAEGAKEELRQEAAVQTFSIIELDGINVRLGDRIYCSVSSFFLEQPDVHSSLIESEEFFTGIKLYPESVTITEDGRQYQLTVESTVPVLCLGAIQLQSECKVTLKFHTVNEAEENRDSDLALSSCQVDLLQSPCQNHSCSQTSLYYTAVTDFMEDGDKLTNIVVDPIVSTNFLWNGYSPQSVQITVKDIPTAYCYSFTDPHVITFDGRSYDNFNTGTFVLYKSASRDFEVHVRQWDCGSLHHPASCNCGFVAKERGDIVAFDMCSGQLHESQPHLSVKSRDKTTTNFKITESYHGRKLTVVFSSGSFIRADVSDWGMSLTLRAPSSDFQNTLGLCGTFDGNADNDFHNSKGDQLEGISNNHLHFINQWKISAGGSLFDKIPQFSQLSQNTQYCNCNPDRLDAFESNKLLNPQTDMFSCHGNDNVRFHALIPGLDITSEYINALDTIRDVSKRSVADSLVYGPLPAAVEQHENIRTVSITEQTDGRIKNTISTKNNGDTKTQGNTNHINRFSPEKKAVKNRFKRQDYQYISTFPYQSLSQTDLEGFSYFFPEDHSTDVPQKFLPSWPTPSGLSHLYVSEFCHQSVFNSTIGKICNDYLKKHIKDVVQMCIVDVLLKDDLSWAEAGVALLENECERNVLELGLHREEFFKQPINDILVALTCPNHCSGNGQCMDWGCACFEGFSSYDCSILEDQVPEILELENKGLCDIRKYACTSVRAFGLGFKETPTLKCEFTKQQYSGGKWIFAEPVLTDAAFRNPRTVDCQLPVDEQQSDGMEPVDDKPIAKWQIKVSNDGYMFSNAKTMTLYDGVCQNCDALSDVLCTLKEKTCNIDGLCYGEGDPNPTSPCLLCRPDVSKLTWSIAENNQPPVIDSFPDKLQTFYGENFVYQFVGADPEGSAILFTFESGPENSSLSPAGLLIWKVTSTVTQEFVFSLTDDCNAQTQVIVEVIVKPCGCINGGSCVTNINFPPGQGEYLCLCPNGFEGDNCQVNIDECKSNPCGPGKCTDEVNGYHCECPLGLKGITCQEDLDECLSSPCYSGVFCTNTYGSYECGQCPDGYQGDGKICTQILLTTQMPLMVLTDIFTKEAGKKELYFPGGSALKSVGQSSFAVPSPTVYSRQTDSRSFQTTRSLSSSLTTSTARRTTISKLLNGRRTTSNLSRSALFASHAIKPSSRGNSTNIYPTAPTVTNSSKHHSVTSGSPAKNKTDEWPFTKHTDNYLPTLNGTDQAVTMASAKRTCADSPCYPGVICEPTISGGFKCGRCPFGYYGDGVTCKAICRHPCGKNMVCAAPNVCRCRNGYSGYNCQTAVCRPDCKNRGKCVRPNVCVCAPGYGGETCEEAYCDQPCENGGTCQARNVCTCPFGYVGPRCETMVCNRHCENGGECVAPDVCKCKPGWYGPTCSTAMCHPVCLNGGTCMKSNICLCPKGFYGAQCQNAVCSPPCKNGGQCMRNNICTCPDGYTGIRCQKSVCNPMCMNGGKCVGPNICSCPSGWKGKQCNTPICLEKCKNGGECVGPNTCHCPAEWEGSQCQTGVSWLREVS

Target ORF information:

RefSeq Version	XM_012965592.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965592.3

ORF Insert Sequence:

ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC 
CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT 
GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG 
CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA 
CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT 
TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG 
AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC 
TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC 
AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA 
GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC 
ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC 
AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA 
ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA 
CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC 
CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA 
GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA 
TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT 
CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT 
TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT 
CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA 
GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT 
GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT 
CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG 
TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC 
GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT 
AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA 
TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC 
TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT 
TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC 
AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA 
TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT 
AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA 
TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA 
ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA 
GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA 
ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT 
CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGTTAAAA ACAGATTTAA GCGGCAAGAT 
TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT 
AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG 
CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC 
AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT 
CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG 
GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA 
TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT 
GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC 
TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT 
GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA 
CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA 
GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT 
GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG 
CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT 
GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA 
ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC 
TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT 
CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT 
CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TTACCTTTGA ATCTGGTCCT 
GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG 
CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG 
GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT 
CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC 
CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA 
GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT 
TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA 
TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG 
ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT 
GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT 
TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA 
ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG 
CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT 
GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG 
ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT 
GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG 
GCAGTTACCA TGGCGTCTGC GAAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT 
ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC 
GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC 
GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT 
GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC 
GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT 
GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA 
TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT 
GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA 
GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC 
TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC 
ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT 
GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC 
CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT 
GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG 
TGCCAGACTG GGGTTTCGTG GTTGAGAGAG GTTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwde ( XM_012965594.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821048.2 Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X3, mRNA.

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CloneID	OXa60325
Clone ID Related Accession (Same CDS sequence)	XM_012965594.3
Accession Version	XM_012965594.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor D and EGF domain-containing protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965594.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCACT GCGGAACACA AGCCCCTGTG TGGCTGTCAT TACGGGACTC TGAGACTCTG 
CCCCCACCTG GGGAGATCCG ACATCTCACC GCCTGTGCGA CTTGGCAGTT TTTCTTCAGT 
AGCACAAAAG ATTGCTGTCT CTTCCGCATC CCTGTGTCAG TGAGAAACTG CGGGGATTTC 
TTTGTATACC TGTTGCAACC TACTCAGGGG TGCATGGGCT ACTGTGCAGA AGTTGTCCCT 
GAGCCTAAAT CAAAGTGTGA TGGAGGAGAA ATAGACACCA ACAATGAATG TACAAGTAAA 
GTAATCGCCA AACCTGTGCA GCCGGAACCT CCAAGTAATC CAGAAATTTC CATTGAGATA 
TCAGGAGACA GTGTACTTTT AAAATGTGAC TTCAAAGCTC CCATTACAAA CAGCACCATG 
GGATTTGTGA TAGGATGGTC AAGACTCTCA GCAGAAGGTG CCAAAGAGGA ACTGAGGCAA 
GAGGCTGCAG TGCAGACATT TTCCATTATA GAACTGGATG GAATTAATGT CAGACTTGGA 
GACCGAATAT ATTGCAGTGT TTCAAGCTTT TTTCTGGAAC AACCAGATGT TCACAGCTCC 
CTAATAGAAA GCGAGGAATT TTTTACTGGC ATAAAGCTAT ACCCCGAGTC TGTCACCATA 
ACGGAAGATG GCAGGCAATA CCAGCTGACA GTAGAGAGCA CAGTGCCAGT TCTGTGCTTA 
GGGGCCATTC AGCTGCAGAG TGAATGTAAA GTCACATTGA AATTCCACAC TGTGAATGAA 
GCTGAGGAAA ATCGTGACTC AGATTTGGCT CTTTCTTCTT GTCAAGTGGA CCTCCTACAA 
TCTCCGTGCC AAAACCATTC CTGCAGCCAG ACTTCTCTTT ATTACACAGC AGTTACAGAT 
TTCATGGAGG ATGGGGATAA ACTCACAAAC ATTGTTGTAG ATCCTATTGT GAGCACTAAC 
TTCCTATGGA ATGGGTATTC TCCGCAGAGT GTGCAGATTA CAGTAAAGGA TATCCCGACT 
GCGTACTGCT ATTCCTTTAC TGATCCACAT GTAATCACAT TTGATGGAAG ATCATATGAC 
AATTTTAATA CAGGAACCTT TGTGCTGTAC AAAAGCGCAT CTCGTGATTT TGAAGTCCAT 
GTGCGCCAGT GGGACTGTGG GAGCCTTCAT CACCCAGCCT CGTGCAACTG TGGCTTTGTT 
GCTAAAGAAA GAGGTGACAT TGTTGCCTTT GATATGTGCA GCGGTCAGCT TCACGAGTCC 
CAGCCGCATT TGTCAGTAAA GAGTCGAGAT AAAACAACGA CTAATTTTAA AATTACAGAA 
TCTTATCATG GAAGGAAATT AACAGTGGTT TTCTCCTCTG GATCTTTTAT CCGAGCTGAT 
GTGAGTGACT GGGGAATGAG TTTAACTCTC AGGGCACCAA GCTCAGATTT TCAGAACACA 
TTGGGACTTT GTGGCACCTT TGATGGAAAT GCAGACAATG ATTTTCATAA CAGCAAAGGA 
GATCAACTAG AAGGGATTTC AAACAACCAC CTTCATTTTA TCAACCAGTG GAAAATTTCC 
GCTGGAGGAA GTTTATTTGA TAAAATCCCA CAATTTTCCC AATTGTCACA AAACACTCAG 
TATTGCAACT GCAATCCAGA CAGATTGGAT GCATTTGAGT CTAACAAACT TCTAAATCCT 
CAGACTGATA TGTTTTCTTG CCATGGAAAT GATAACGTTC GATTCCATGC ACTCATACCA 
GGTTTGGATA TCACATCTGA ATACATAAAT GCCCTTGATA CAATCCGAGA CGTAAGCAAG 
CGTTCTGTTG CTGATAGCCT TGTCTACGGA CCATTACCTG CAGCAGTTGA ACAGCATGAA 
AATATCAGAA CTGTTAGCAT TACTGAGCAA ACTGATGGAA GAATAAAAAA CACAATCTCC 
ACAAAAAATA ATGGAGATAC AAAAACCCAA GGCAATACAA ATCACATCAA TAGATTTAGT 
CCTGAAAAGA AAGCTGTTAA AAACAGATTT AAGCGGCAAG ATTATCAATA CATTTCAACA 
TTTCCATATC AAAGCCTTAG CCAAACAGAC TTGGAAGGTT TTAGCTACTT TTTTCCTGAA 
GACCACTCCA CAGATGTTCC TCAGAAATTT TTGCCTTCTT GGCCAACACC GTCAGGCCTG 
AGTCATTTGT ATGTTTCCGA ATTTTGCCAT CAATCGGTTT TCAACTCAAC CATTGGAAAA 
ATATGCAATG ATTATCTAAA GAAACATATC AAAGATGTAG TTCAGATGTG CATTGTGGAC 
GTACTGTTAA AGGATGACCT GAGCTGGGCA GAGGCTGGAG TGGCCCTGCT GGAAAATGAA 
TGTGAAAGAA ATGTTCTAGA ATTAGGACTT CACAGAGAAG AATTCTTCAA ACAACCCATC 
AATGATATTT TGGTAGCCCT GACATGCCCT AATCACTGCA GTGGTAATGG ACAATGTATG 
GACTGGGGAT GTGCTTGCTT CGAAGGTTTC AGTTCCTACG ACTGTAGCAT TTTGGAAGAT 
CAGGTGCCTG AGATTTTGGA ACTTGAAAAT AAGGGATTGT GTGACATTCG AAAATATGCC 
TGCACTTCTG TAAGGGCATT CGGTCTTGGC TTTAAAGAAA CACCGACCCT GAAGTGTGAA 
TTTACTAAGC AGCAGTATAG TGGTGGAAAG TGGATTTTTG CAGAGCCAGT GCTAACAGAT 
GCTGCCTTCA GGAATCCTAG AACTGTAGAC TGTCAATTGC CTGTAGATGA GCAACAGTCT 
GATGGCATGG AGCCAGTGGA TGATAAACCT ATAGCTAAAT GGCAAATTAA GGTATCTAAT 
GATGGTTATA TGTTTAGTAA TGCTAAAACA ATGACCTTAT ATGATGGAGT ATGCCAGAAT 
TGTGATGCGC TGTCAGATGT GCTGTGTACA TTAAAGGAGA AAACATGTAA TATAGATGGA 
CTCTGCTACG GAGAGGGTGA TCCCAATCCT ACAAGCCCAT GCTTACTGTG CAGACCAGAT 
GTGTCAAAGC TAACATGGTC TATTGCTGAA AATAACCAGC CTCCTGTGAT TGATTCTTTC 
CCAGATAAAT TACAAACTTT CTATGGGGAA AACTTTGTGT ATCAATTTGT GGGAGCAGAT 
CCAGAAGGTT CTGCCATCCT TTTTACCTTT GAATCTGGTC CTGAAAATTC CAGCTTATCC 
CCCGCAGGGT TGCTCATATG GAAAGTTACG TCGACGGTCA CGCAGGAATT TGTGTTTTCT 
CTAACAGATG ACTGCAATGC TCAGACTCAA GTAATTGTGG AGGTCATAGT AAAACCCTGT 
GGCTGCATTA ATGGCGGCTC ATGTGTAACC AACATTAACT TTCCACCTGG ACAAGGAGAA 
TATCTGTGTC TTTGCCCAAA TGGGTTTGAG GGGGATAACT GCCAAGTAAA CATAGATGAA 
TGTAAGTCTA ATCCATGCGG TCCTGGTAAA TGTACCGATG AAGTGAACGG CTACCATTGT 
GAATGTCCTT TGGGGCTAAA AGGAATAACA TGCCAAGAAG ATTTGGATGA GTGTTTATCC 
AGCCCTTGTT ACTCTGGTGT ATTTTGCACA AATACTTATG GATCATATGA ATGTGGGCAA 
TGCCCAGATG GCTATCAAGG AGATGGAAAG ATATGTACAC AGATATTATT GACAACACAG 
ATGCCTCTAA TGGTGTTAAC GGATATCTTT ACCAAAGAAG CTGGGAAAAA AGAACTTTAC 
TTCCCTGGAG GTTCAGCATT AAAGTCAGTA GGCCAGTCAA GTTTTGCTGT TCCAAGTCCA 
ACAGTATATA GCAGGCAAAC AGACAGCCGC TCCTTCCAAA CAACTCGGAG TCTTTCATCA 
AGCTTAACAA CCAGTACCGC AAGAAGAACG ACTATTTCTA AGCTACTAAA TGGTCGAAGG 
ACCACCTCCA ACCTATCTCG CTCAGCTTTG TTTGCCAGTC ATGCAATAAA GCCAAGTTCC 
AGAGGGAACA GTACTAATAT ATATCCTACT GCACCAACAG TGACTAACAG CTCAAAGCAT 
CACTCTGTTA CTTCAGGAAG CCCAGCAAAG AACAAGACTG ATGAATGGCC ATTTACAAAA 
CATACAGATA ACTATTTACC GACATTGAAT GGAACAGATC AGGCAGTTAC CATGGCGTCT 
GCGAAACGGA CATGTGCTGA TTCACCCTGT TATCCTGGAG TTATTTGTGA ACCAACCATC 
AGCGGAGGCT TTAAATGTGG ACGATGTCCT TTTGGCTACT ACGGAGATGG TGTAACATGC 
AAGGCAATTT GCCGGCATCC ATGTGGAAAG AATATGGTAT GCGCTGCTCC AAATGTTTGC 
AGATGTAGAA ATGGCTACTC TGGATACAAC TGCCAGACTG CTGTGTGTCG TCCTGACTGT 
AAGAATCGTG GGAAATGTGT GAGACCCAAT GTTTGTGTAT GCGCCCCAGG ATATGGTGGA 
GAAACATGTG AAGAAGCCTA CTGTGACCAA CCATGTGAAA ATGGAGGGAC CTGCCAGGCA 
AGAAATGTCT GCACCTGCCC ATTTGGTTAT GTTGGACCCA GATGTGAAAC AATGGTCTGT 
AATCGGCACT GTGAAAATGG TGGCGAGTGT GTCGCTCCAG ATGTGTGCAA GTGCAAACCT 
GGGTGGTACG GCCCAACATG CAGCACAGCC ATGTGTCATC CAGTTTGTCT TAATGGCGGC 
ACTTGCATGA AATCCAATAT TTGTCTGTGT CCCAAAGGAT TCTATGGTGC CCAGTGTCAG 
AATGCGGTCT GTAGCCCTCC TTGCAAAAAT GGCGGCCAGT GCATGAGAAA TAACATTTGC 
ACGTGCCCAG ATGGATACAC TGGAATAAGA TGCCAGAAAA GTGTCTGCAA TCCAATGTGC 
ATGAATGGAG GGAAATGTGT GGGGCCCAAT ATTTGTTCTT GCCCATCAGG ATGGAAAGGG 
AAGCAATGCA ATACACCTAT ATGCCTTGAA AAATGTAAGA ATGGAGGTGA GTGTGTGGGA 
CCCAATACCT GCCACTGTCC AGCAGAGTGG GAAGGAAGCC AGTGCCAGAC TGCTGTCTGC 
AACTACAAAT GTTTATATGG AGGCCGCTGT GTACTGCCCA ATGTATGCTC ATGTCGCGCA 
GGATACACTG GAACAACTTG TAACCAGAGA GTACAGGGGT TTCGTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821048.2
CDS	90..5240
Translation	MNHCGTQAPVWLSLRDSETLPPPGEIRHLTACATWQFFFSSTKDCCLFRIPVSVRNCGDFFVYLLQPTQGCMGYCAEVVPEPKSKCDGGEIDTNNECTSKVIAKPVQPEPPSNPEISIEISGDSVLLKCDFKAPITNSTMGFVIGWSRLSAEGAKEELRQEAAVQTFSIIELDGINVRLGDRIYCSVSSFFLEQPDVHSSLIESEEFFTGIKLYPESVTITEDGRQYQLTVESTVPVLCLGAIQLQSECKVTLKFHTVNEAEENRDSDLALSSCQVDLLQSPCQNHSCSQTSLYYTAVTDFMEDGDKLTNIVVDPIVSTNFLWNGYSPQSVQITVKDIPTAYCYSFTDPHVITFDGRSYDNFNTGTFVLYKSASRDFEVHVRQWDCGSLHHPASCNCGFVAKERGDIVAFDMCSGQLHESQPHLSVKSRDKTTTNFKITESYHGRKLTVVFSSGSFIRADVSDWGMSLTLRAPSSDFQNTLGLCGTFDGNADNDFHNSKGDQLEGISNNHLHFINQWKISAGGSLFDKIPQFSQLSQNTQYCNCNPDRLDAFESNKLLNPQTDMFSCHGNDNVRFHALIPGLDITSEYINALDTIRDVSKRSVADSLVYGPLPAAVEQHENIRTVSITEQTDGRIKNTISTKNNGDTKTQGNTNHINRFSPEKKAVKNRFKRQDYQYISTFPYQSLSQTDLEGFSYFFPEDHSTDVPQKFLPSWPTPSGLSHLYVSEFCHQSVFNSTIGKICNDYLKKHIKDVVQMCIVDVLLKDDLSWAEAGVALLENECERNVLELGLHREEFFKQPINDILVALTCPNHCSGNGQCMDWGCACFEGFSSYDCSILEDQVPEILELENKGLCDIRKYACTSVRAFGLGFKETPTLKCEFTKQQYSGGKWIFAEPVLTDAAFRNPRTVDCQLPVDEQQSDGMEPVDDKPIAKWQIKVSNDGYMFSNAKTMTLYDGVCQNCDALSDVLCTLKEKTCNIDGLCYGEGDPNPTSPCLLCRPDVSKLTWSIAENNQPPVIDSFPDKLQTFYGENFVYQFVGADPEGSAILFTFESGPENSSLSPAGLLIWKVTSTVTQEFVFSLTDDCNAQTQVIVEVIVKPCGCINGGSCVTNINFPPGQGEYLCLCPNGFEGDNCQVNIDECKSNPCGPGKCTDEVNGYHCECPLGLKGITCQEDLDECLSSPCYSGVFCTNTYGSYECGQCPDGYQGDGKICTQILLTTQMPLMVLTDIFTKEAGKKELYFPGGSALKSVGQSSFAVPSPTVYSRQTDSRSFQTTRSLSSSLTTSTARRTTISKLLNGRRTTSNLSRSALFASHAIKPSSRGNSTNIYPTAPTVTNSSKHHSVTSGSPAKNKTDEWPFTKHTDNYLPTLNGTDQAVTMASAKRTCADSPCYPGVICEPTISGGFKCGRCPFGYYGDGVTCKAICRHPCGKNMVCAAPNVCRCRNGYSGYNCQTAVCRPDCKNRGKCVRPNVCVCAPGYGGETCEEAYCDQPCENGGTCQARNVCTCPFGYVGPRCETMVCNRHCENGGECVAPDVCKCKPGWYGPTCSTAMCHPVCLNGGTCMKSNICLCPKGFYGAQCQNAVCSPPCKNGGQCMRNNICTCPDGYTGIRCQKSVCNPMCMNGGKCVGPNICSCPSGWKGKQCNTPICLEKCKNGGECVGPNTCHCPAEWEGSQCQTAVCNYKCLYGGRCVLPNVCSCRAGYTGTTCNQRVQGFRG

Target ORF information:

RefSeq Version	XM_012965594.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965594.3

ORF Insert Sequence:

ATGAATCACT GCGGAACACA AGCCCCTGTG TGGCTGTCAT TACGGGACTC TGAGACTCTG 
CCCCCACCTG GGGAGATCCG ACATCTCACC GCCTGTGCGA CTTGGCAGTT TTTCTTCAGT 
AGCACAAAAG ATTGCTGTCT CTTCCGCATC CCTGTGTCAG TGAGAAACTG CGGGGATTTC 
TTTGTATACC TGTTGCAACC TACTCAGGGG TGCATGGGCT ACTGTGCAGA AGTTGTCCCT 
GAGCCTAAAT CAAAGTGTGA TGGAGGAGAA ATAGACACCA ACAATGAATG TACAAGTAAA 
GTAATCGCCA AACCTGTGCA GCCGGAACCT CCAAGTAATC CAGAAATTTC CATTGAGATA 
TCAGGAGACA GTGTACTTTT AAAATGTGAC TTCAAAGCTC CCATTACAAA CAGCACCATG 
GGATTTGTGA TAGGATGGTC AAGACTCTCA GCAGAAGGTG CCAAAGAGGA ACTGAGGCAA 
GAGGCTGCAG TGCAGACATT TTCCATTATA GAACTGGATG GAATTAATGT CAGACTTGGA 
GACCGAATAT ATTGCAGTGT TTCAAGCTTT TTTCTGGAAC AACCAGATGT TCACAGCTCC 
CTAATAGAAA GCGAGGAATT TTTTACTGGC ATAAAGCTAT ACCCCGAGTC TGTCACCATA 
ACGGAAGATG GCAGGCAATA CCAGCTGACA GTAGAGAGCA CAGTGCCAGT TCTGTGCTTA 
GGGGCCATTC AGCTGCAGAG TGAATGTAAA GTCACATTGA AATTCCACAC TGTGAATGAA 
GCTGAGGAAA ATCGTGACTC AGATTTGGCT CTTTCTTCTT GTCAAGTGGA CCTCCTACAA 
TCTCCGTGCC AAAACCATTC CTGCAGCCAG ACTTCTCTTT ATTACACAGC AGTTACAGAT 
TTCATGGAGG ATGGGGATAA ACTCACAAAC ATTGTTGTAG ATCCTATTGT GAGCACTAAC 
TTCCTATGGA ATGGGTATTC TCCGCAGAGT GTGCAGATTA CAGTAAAGGA TATCCCGACT 
GCGTACTGCT ATTCCTTTAC TGATCCACAT GTAATCACAT TTGATGGAAG ATCATATGAC 
AATTTTAATA CAGGAACCTT TGTGCTGTAC AAAAGCGCAT CTCGTGATTT TGAAGTCCAT 
GTGCGCCAGT GGGACTGTGG GAGCCTTCAT CACCCAGCCT CGTGCAACTG TGGCTTTGTT 
GCTAAAGAAA GAGGTGACAT TGTTGCCTTT GATATGTGCA GCGGTCAGCT TCACGAGTCC 
CAGCCGCATT TGTCAGTAAA GAGTCGAGAT AAAACAACGA CTAATTTTAA AATTACAGAA 
TCTTATCATG GAAGGAAATT AACAGTGGTT TTCTCCTCTG GATCTTTTAT CCGAGCTGAT 
GTGAGTGACT GGGGAATGAG TTTAACTCTC AGGGCACCAA GCTCAGATTT TCAGAACACA 
TTGGGACTTT GTGGCACCTT TGATGGAAAT GCAGACAATG ATTTTCATAA CAGCAAAGGA 
GATCAACTAG AAGGGATTTC AAACAACCAC CTTCATTTTA TCAACCAGTG GAAAATTTCC 
GCTGGAGGAA GTTTATTTGA TAAAATCCCA CAATTTTCCC AATTGTCACA AAACACTCAG 
TATTGCAACT GCAATCCAGA CAGATTGGAT GCATTTGAGT CTAACAAACT TCTAAATCCT 
CAGACTGATA TGTTTTCTTG CCATGGAAAT GATAACGTTC GATTCCATGC ACTCATACCA 
GGTTTGGATA TCACATCTGA ATACATAAAT GCCCTTGATA CAATCCGAGA CGTAAGCAAG 
CGTTCTGTTG CTGATAGCCT TGTCTACGGA CCATTACCTG CAGCAGTTGA ACAGCATGAA 
AATATCAGAA CTGTTAGCAT TACTGAGCAA ACTGATGGAA GAATAAAAAA CACAATCTCC 
ACAAAAAATA ATGGAGATAC AAAAACCCAA GGCAATACAA ATCACATCAA TAGATTTAGT 
CCTGAAAAGA AAGCTGTTAA AAACAGATTT AAGCGGCAAG ATTATCAATA CATTTCAACA 
TTTCCATATC AAAGCCTTAG CCAAACAGAC TTGGAAGGTT TTAGCTACTT TTTTCCTGAA 
GACCACTCCA CAGATGTTCC TCAGAAATTT TTGCCTTCTT GGCCAACACC GTCAGGCCTG 
AGTCATTTGT ATGTTTCCGA ATTTTGCCAT CAATCGGTTT TCAACTCAAC CATTGGAAAA 
ATATGCAATG ATTATCTAAA GAAACATATC AAAGATGTAG TTCAGATGTG CATTGTGGAC 
GTACTGTTAA AGGATGACCT GAGCTGGGCA GAGGCTGGAG TGGCCCTGCT GGAAAATGAA 
TGTGAAAGAA ATGTTCTAGA ATTAGGACTT CACAGAGAAG AATTCTTCAA ACAACCCATC 
AATGATATTT TGGTAGCCCT GACATGCCCT AATCACTGCA GTGGTAATGG ACAATGTATG 
GACTGGGGAT GTGCTTGCTT CGAAGGTTTC AGTTCCTACG ACTGTAGCAT TTTGGAAGAT 
CAGGTGCCTG AGATTTTGGA ACTTGAAAAT AAGGGATTGT GTGACATTCG AAAATATGCC 
TGCACTTCTG TAAGGGCATT CGGTCTTGGC TTTAAAGAAA CACCGACCCT GAAGTGTGAA 
TTTACTAAGC AGCAGTATAG TGGTGGAAAG TGGATTTTTG CAGAGCCAGT GCTAACAGAT 
GCTGCCTTCA GGAATCCTAG AACTGTAGAC TGTCAATTGC CTGTAGATGA GCAACAGTCT 
GATGGCATGG AGCCAGTGGA TGATAAACCT ATAGCTAAAT GGCAAATTAA GGTATCTAAT 
GATGGTTATA TGTTTAGTAA TGCTAAAACA ATGACCTTAT ATGATGGAGT ATGCCAGAAT 
TGTGATGCGC TGTCAGATGT GCTGTGTACA TTAAAGGAGA AAACATGTAA TATAGATGGA 
CTCTGCTACG GAGAGGGTGA TCCCAATCCT ACAAGCCCAT GCTTACTGTG CAGACCAGAT 
GTGTCAAAGC TAACATGGTC TATTGCTGAA AATAACCAGC CTCCTGTGAT TGATTCTTTC 
CCAGATAAAT TACAAACTTT CTATGGGGAA AACTTTGTGT ATCAATTTGT GGGAGCAGAT 
CCAGAAGGTT CTGCCATCCT TTTTACCTTT GAATCTGGTC CTGAAAATTC CAGCTTATCC 
CCCGCAGGGT TGCTCATATG GAAAGTTACG TCGACGGTCA CGCAGGAATT TGTGTTTTCT 
CTAACAGATG ACTGCAATGC TCAGACTCAA GTAATTGTGG AGGTCATAGT AAAACCCTGT 
GGCTGCATTA ATGGCGGCTC ATGTGTAACC AACATTAACT TTCCACCTGG ACAAGGAGAA 
TATCTGTGTC TTTGCCCAAA TGGGTTTGAG GGGGATAACT GCCAAGTAAA CATAGATGAA 
TGTAAGTCTA ATCCATGCGG TCCTGGTAAA TGTACCGATG AAGTGAACGG CTACCATTGT 
GAATGTCCTT TGGGGCTAAA AGGAATAACA TGCCAAGAAG ATTTGGATGA GTGTTTATCC 
AGCCCTTGTT ACTCTGGTGT ATTTTGCACA AATACTTATG GATCATATGA ATGTGGGCAA 
TGCCCAGATG GCTATCAAGG AGATGGAAAG ATATGTACAC AGATATTATT GACAACACAG 
ATGCCTCTAA TGGTGTTAAC GGATATCTTT ACCAAAGAAG CTGGGAAAAA AGAACTTTAC 
TTCCCTGGAG GTTCAGCATT AAAGTCAGTA GGCCAGTCAA GTTTTGCTGT TCCAAGTCCA 
ACAGTATATA GCAGGCAAAC AGACAGCCGC TCCTTCCAAA CAACTCGGAG TCTTTCATCA 
AGCTTAACAA CCAGTACCGC AAGAAGAACG ACTATTTCTA AGCTACTAAA TGGTCGAAGG 
ACCACCTCCA ACCTATCTCG CTCAGCTTTG TTTGCCAGTC ATGCAATAAA GCCAAGTTCC 
AGAGGGAACA GTACTAATAT ATATCCTACT GCACCAACAG TGACTAACAG CTCAAAGCAT 
CACTCTGTTA CTTCAGGAAG CCCAGCAAAG AACAAGACTG ATGAATGGCC ATTTACAAAA 
CATACAGATA ACTATTTACC GACATTGAAT GGAACAGATC AGGCAGTTAC CATGGCGTCT 
GCGAAACGGA CATGTGCTGA TTCACCCTGT TATCCTGGAG TTATTTGTGA ACCAACCATC 
AGCGGAGGCT TTAAATGTGG ACGATGTCCT TTTGGCTACT ACGGAGATGG TGTAACATGC 
AAGGCAATTT GCCGGCATCC ATGTGGAAAG AATATGGTAT GCGCTGCTCC AAATGTTTGC 
AGATGTAGAA ATGGCTACTC TGGATACAAC TGCCAGACTG CTGTGTGTCG TCCTGACTGT 
AAGAATCGTG GGAAATGTGT GAGACCCAAT GTTTGTGTAT GCGCCCCAGG ATATGGTGGA 
GAAACATGTG AAGAAGCCTA CTGTGACCAA CCATGTGAAA ATGGAGGGAC CTGCCAGGCA 
AGAAATGTCT GCACCTGCCC ATTTGGTTAT GTTGGACCCA GATGTGAAAC AATGGTCTGT 
AATCGGCACT GTGAAAATGG TGGCGAGTGT GTCGCTCCAG ATGTGTGCAA GTGCAAACCT 
GGGTGGTACG GCCCAACATG CAGCACAGCC ATGTGTCATC CAGTTTGTCT TAATGGCGGC 
ACTTGCATGA AATCCAATAT TTGTCTGTGT CCCAAAGGAT TCTATGGTGC CCAGTGTCAG 
AATGCGGTCT GTAGCCCTCC TTGCAAAAAT GGCGGCCAGT GCATGAGAAA TAACATTTGC 
ACGTGCCCAG ATGGATACAC TGGAATAAGA TGCCAGAAAA GTGTCTGCAA TCCAATGTGC 
ATGAATGGAG GGAAATGTGT GGGGCCCAAT ATTTGTTCTT GCCCATCAGG ATGGAAAGGG 
AAGCAATGCA ATACACCTAT ATGCCTTGAA AAATGTAAGA ATGGAGGTGA GTGTGTGGGA 
CCCAATACCT GCCACTGTCC AGCAGAGTGG GAAGGAAGCC AGTGCCAGAC TGCTGTCTGC 
AACTACAAAT GTTTATATGG AGGCCGCTGT GTACTGCCCA ATGTATGCTC ATGTCGCGCA 
GGATACACTG GAACAACTTG TAACCAGAGA GTACAGGGGT TTCGTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwde ( XM_031903928.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759788.1 Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa60326
Clone ID Related Accession (Same CDS sequence)	XM_031903928.1
Accession Version	XM_031903928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor D and EGF domain-containing protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC 
CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT 
GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG 
CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA 
CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT 
TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG 
AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC 
TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC 
AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA 
GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC 
ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC 
AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA 
ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA 
CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC 
CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA 
GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA 
TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT 
CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT 
TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT 
CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA 
GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT 
GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT 
CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG 
TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC 
GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT 
AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA 
TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC 
TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT 
TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC 
AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA 
TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT 
AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA 
TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA 
ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA 
GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA 
ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT 
CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGTTAAAA ACAGATTTAA GCGGCAAGAT 
TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT 
AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG 
CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC 
AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT 
CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG 
GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA 
TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT 
GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC 
TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT 
GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA 
CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA 
GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT 
GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG 
CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT 
GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA 
ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC 
TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT 
CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT 
CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TTACCTTTGA ATCTGGTCCT 
GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG 
CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG 
GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT 
CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC 
CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA 
GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT 
TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA 
TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG 
ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT 
GGGAAAAAAG AACTTTACTT CCCTGGAGCA ATTTGCCGGC ATCCATGTGG AAAGAATATG 
GTATGCGCTG CTCCAAATGT TTGCAGATGT AGAAATGGCT ACTCTGGATA CAACTGCCAG 
ACTGCTGTGT GTCGTCCTGA CTGTAAGAAT CGTGGGAAAT GTGTGAGACC CAATGTTTGT 
GTATGCGCCC CAGGATATGG TGGAGAAACA TGTGAAGAAG CCTACTGTGA CCAACCATGT 
GAAAATGGAG GGACCTGCCA GGCAAGAAAT GTCTGCACCT GCCCATTTGG TTATGTTGGA 
CCCAGATGTG AAACAATGGT CTGTAATCGG CACTGTGAAA ATGGTGGCGA GTGTGTCGCT 
CCAGATGTGT GCAAGTGCAA ACCTGGGTGG TACGGCCCAA CATGCAGCAC AGCCATGTGT 
CATCCAGTTT GTCTTAATGG CGGCACTTGC ATGAAATCCA ATATTTGTCT GTGTCCCAAA 
GGATTCTATG GTGCCCAGTG TCAGAATGCG GTCTGTAGCC CTCCTTGCAA AAATGGCGGC 
CAGTGCATGA GAAATAACAT TTGCACGTGC CCAGATGGAT ACACTGGAAT AAGATGCCAG 
AAAAGTGTCT GCAATCCAAT GTGCATGAAT GGAGGGAAAT GTGTGGGGCC CAATATTTGT 
TCTTGCCCAT CAGGATGGAA AGGGAAGCAA TGCAATACAC CTATATGCCT TGAAAAATGT 
AAGAATGGAG GTGAGTGTGT GGGACCCAAT ACCTGCCACT GTCCAGCAGA GTGGGAAGGA 
AGCCAGTGCC AGACTGCTGT CTGCAACTAC AAATGTTTAT ATGGAGGCCG CTGTGTACTG 
CCCAATGTAT GCTCATGTCG CGCAGGATAC ACTGGAACAA CTTGTAACCA GAGAGTACAG 
GGGTTTCGTG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759788.1
CDS	242..5116
Translation	MSLNLQPGCATLWVLLLILQTAAAQQECSSSGHQILQNSYRSLDFDSLKLQQSAIHDVICDHSLSPGWYRFMIFDKPAEMPTKCVEMNHCGTQAPVWLSLRDSETLPPPGEIRHLTACATWQFFFSSTKDCCLFRIPVSVRNCGDFFVYLLQPTQGCMGYCAEVVPEPKSKCDGGEIDTNNECTSKVIAKPVQPEPPSNPEISIEISGDSVLLKCDFKAPITNSTMGFVIGWSRLSAEGAKEELRQEAAVQTFSIIELDGINVRLGDRIYCSVSSFFLEQPDVHSSLIESEEFFTGIKLYPESVTITEDGRQYQLTVESTVPVLCLGAIQLQSECKVTLKFHTVNEAEENRDSDLALSSCQVDLLQSPCQNHSCSQTSLYYTAVTDFMEDGDKLTNIVVDPIVSTNFLWNGYSPQSVQITVKDIPTAYCYSFTDPHVITFDGRSYDNFNTGTFVLYKSASRDFEVHVRQWDCGSLHHPASCNCGFVAKERGDIVAFDMCSGQLHESQPHLSVKSRDKTTTNFKITESYHGRKLTVVFSSGSFIRADVSDWGMSLTLRAPSSDFQNTLGLCGTFDGNADNDFHNSKGDQLEGISNNHLHFINQWKISAGGSLFDKIPQFSQLSQNTQYCNCNPDRLDAFESNKLLNPQTDMFSCHGNDNVRFHALIPGLDITSEYINALDTIRDVSKRSVADSLVYGPLPAAVEQHENIRTVSITEQTDGRIKNTISTKNNGDTKTQGNTNHINRFSPEKKAVKNRFKRQDYQYISTFPYQSLSQTDLEGFSYFFPEDHSTDVPQKFLPSWPTPSGLSHLYVSEFCHQSVFNSTIGKICNDYLKKHIKDVVQMCIVDVLLKDDLSWAEAGVALLENECERNVLELGLHREEFFKQPINDILVALTCPNHCSGNGQCMDWGCACFEGFSSYDCSILEDQVPEILELENKGLCDIRKYACTSVRAFGLGFKETPTLKCEFTKQQYSGGKWIFAEPVLTDAAFRNPRTVDCQLPVDEQQSDGMEPVDDKPIAKWQIKVSNDGYMFSNAKTMTLYDGVCQNCDALSDVLCTLKEKTCNIDGLCYGEGDPNPTSPCLLCRPDVSKLTWSIAENNQPPVIDSFPDKLQTFYGENFVYQFVGADPEGSAILFTFESGPENSSLSPAGLLIWKVTSTVTQEFVFSLTDDCNAQTQVIVEVIVKPCGCINGGSCVTNINFPPGQGEYLCLCPNGFEGDNCQVNIDECKSNPCGPGKCTDEVNGYHCECPLGLKGITCQEDLDECLSSPCYSGVFCTNTYGSYECGQCPDGYQGDGKICTQILLTTQMPLMVLTDIFTKEAGKKELYFPGAICRHPCGKNMVCAAPNVCRCRNGYSGYNCQTAVCRPDCKNRGKCVRPNVCVCAPGYGGETCEEAYCDQPCENGGTCQARNVCTCPFGYVGPRCETMVCNRHCENGGECVAPDVCKCKPGWYGPTCSTAMCHPVCLNGGTCMKSNICLCPKGFYGAQCQNAVCSPPCKNGGQCMRNNICTCPDGYTGIRCQKSVCNPMCMNGGKCVGPNICSCPSGWKGKQCNTPICLEKCKNGGECVGPNTCHCPAEWEGSQCQTAVCNYKCLYGGRCVLPNVCSCRAGYTGTTCNQRVQGFRG

Target ORF information:

RefSeq Version	XM_031903928.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903928.1

ORF Insert Sequence:

ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC 
CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT 
GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG 
CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA 
CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT 
TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG 
AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC 
TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC 
AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA 
GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC 
ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC 
AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA 
ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA 
CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC 
CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA 
GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA 
TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT 
CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT 
TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT 
CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA 
GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT 
GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT 
CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG 
TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC 
GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT 
AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA 
TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC 
TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT 
TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC 
AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA 
TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT 
AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA 
TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA 
ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA 
GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA 
ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT 
CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGTTAAAA ACAGATTTAA GCGGCAAGAT 
TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT 
AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG 
CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC 
AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT 
CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG 
GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA 
TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT 
GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC 
TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT 
GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA 
CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA 
GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT 
GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG 
CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT 
GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA 
ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC 
TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT 
CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT 
CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TTACCTTTGA ATCTGGTCCT 
GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG 
CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG 
GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT 
CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC 
CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA 
GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT 
TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA 
TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG 
ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT 
GGGAAAAAAG AACTTTACTT CCCTGGAGCA ATTTGCCGGC ATCCATGTGG AAAGAATATG 
GTATGCGCTG CTCCAAATGT TTGCAGATGT AGAAATGGCT ACTCTGGATA CAACTGCCAG 
ACTGCTGTGT GTCGTCCTGA CTGTAAGAAT CGTGGGAAAT GTGTGAGACC CAATGTTTGT 
GTATGCGCCC CAGGATATGG TGGAGAAACA TGTGAAGAAG CCTACTGTGA CCAACCATGT 
GAAAATGGAG GGACCTGCCA GGCAAGAAAT GTCTGCACCT GCCCATTTGG TTATGTTGGA 
CCCAGATGTG AAACAATGGT CTGTAATCGG CACTGTGAAA ATGGTGGCGA GTGTGTCGCT 
CCAGATGTGT GCAAGTGCAA ACCTGGGTGG TACGGCCCAA CATGCAGCAC AGCCATGTGT 
CATCCAGTTT GTCTTAATGG CGGCACTTGC ATGAAATCCA ATATTTGTCT GTGTCCCAAA 
GGATTCTATG GTGCCCAGTG TCAGAATGCG GTCTGTAGCC CTCCTTGCAA AAATGGCGGC 
CAGTGCATGA GAAATAACAT TTGCACGTGC CCAGATGGAT ACACTGGAAT AAGATGCCAG 
AAAAGTGTCT GCAATCCAAT GTGCATGAAT GGAGGGAAAT GTGTGGGGCC CAATATTTGT 
TCTTGCCCAT CAGGATGGAA AGGGAAGCAA TGCAATACAC CTATATGCCT TGAAAAATGT 
AAGAATGGAG GTGAGTGTGT GGGACCCAAT ACCTGCCACT GTCCAGCAGA GTGGGAAGGA 
AGCCAGTGCC AGACTGCTGT CTGCAACTAC AAATGTTTAT ATGGAGGCCG CTGTGTACTG 
CCCAATGTAT GCTCATGTCG CGCAGGATAC ACTGGAACAA CTTGTAACCA GAGAGTACAG 
GGGTTTCGTG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwde ( XM_002939011.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939057.2 Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X1, mRNA.

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CloneID	OXa12410
Clone ID Related Accession (Same CDS sequence)	XM_002939011.4
Accession Version	XM_002939011.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor D and EGF domain-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002939011.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC 
CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT 
GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG 
CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA 
CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT 
TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG 
AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC 
TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC 
AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA 
GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC 
ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC 
AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA 
ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA 
CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC 
CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA 
GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA 
TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT 
CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT 
TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT 
CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA 
GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT 
GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT 
CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG 
TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC 
GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT 
AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA 
TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC 
TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT 
TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC 
AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA 
TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT 
AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA 
TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA 
ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA 
GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA 
ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT 
CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGATAAAA ACAGATTTAA GCGGCAAGAT 
TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT 
AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG 
CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC 
AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT 
CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG 
GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA 
TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT 
GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC 
TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT 
GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA 
CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA 
GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT 
GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG 
CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT 
GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA 
ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC 
TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT 
CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT 
CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TCACCTTTGA ATCTGGTCCT 
GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG 
CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG 
GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT 
CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC 
CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA 
GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT 
TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA 
TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG 
ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT 
GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT 
TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA 
ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG 
CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT 
GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG 
ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT 
GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG 
GCAGTTACCA TGGCGTCTGT GGAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT 
ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC 
GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC 
GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT 
GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC 
GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT 
GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA 
TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT 
GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA 
GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC 
TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC 
ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT 
GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC 
CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT 
GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG 
TGCCAGACTG CTGTCTGCAA CTACAAATGT TTATATGGAG GCCGCTGTGT ACTGCCCAAT 
GTATGCTCAT GTCGCGCAGG ATACACTGGA ACGACTTGTA ACCAGAGAGT ACAGGGGTTT 
CGTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939057.2
CDS	217..5625
Translation	MSLNLQPGCATLWVLLLILQTAAAQQECSSSGHQILQNSYRSLDFDSLKLQQSAIHDVICDHSLSPGWYRFMIFDKPAEMPTKCVEMNHCGTQAPVWLSLRDSETLPPPGEIRHLTACATWQFFFSSTKDCCLFRIPVSVRNCGDFFVYLLQPTQGCMGYCAEVVPEPKSKCDGGEIDTNNECTSKVIAKPVQPEPPSNPEISIEISGDSVLLKCDFKAPITNSTMGFVIGWSRLSAEGAKEELRQEAAVQTFSIIELDGINVRLGDRIYCSVSSFFLEQPDVHSSLIESEEFFTGIKLYPESVTITEDGRQYQLTVESTVPVLCLGAIQLQSECKVTLKFHTVNEAEENRDSDLALSSCQVDLLQSPCQNHSCSQTSLYYTAVTDFMEDGDKLTNIVVDPIVSTNFLWNGYSPQSVQITVKDIPTAYCYSFTDPHVITFDGRSYDNFNTGTFVLYKSASRDFEVHVRQWDCGSLHHPASCNCGFVAKERGDIVAFDMCSGQLHESQPHLSVKSRDKTTTNFKITESYHGRKLTVVFSSGSFIRADVSDWGMSLTLRAPSSDFQNTLGLCGTFDGNADNDFHNSKGDQLEGISNNHLHFINQWKISAGGSLFDKIPQFSQLSQNTQYCNCNPDRLDAFESNKLLNPQTDMFSCHGNDNVRFHALIPGLDITSEYINALDTIRDVSKRSVADSLVYGPLPAAVEQHENIRTVSITEQTDGRIKNTISTKNNGDTKTQGNTNHINRFSPEKKADKNRFKRQDYQYISTFPYQSLSQTDLEGFSYFFPEDHSTDVPQKFLPSWPTPSGLSHLYVSEFCHQSVFNSTIGKICNDYLKKHIKDVVQMCIVDVLLKDDLSWAEAGVALLENECERNVLELGLHREEFFKQPINDILVALTCPNHCSGNGQCMDWGCACFEGFSSYDCSILEDQVPEILELENKGLCDIRKYACTSVRAFGLGFKETPTLKCEFTKQQYSGGKWIFAEPVLTDAAFRNPRTVDCQLPVDEQQSDGMEPVDDKPIAKWQIKVSNDGYMFSNAKTMTLYDGVCQNCDALSDVLCTLKEKTCNIDGLCYGEGDPNPTSPCLLCRPDVSKLTWSIAENNQPPVIDSFPDKLQTFYGENFVYQFVGADPEGSAILFTFESGPENSSLSPAGLLIWKVTSTVTQEFVFSLTDDCNAQTQVIVEVIVKPCGCINGGSCVTNINFPPGQGEYLCLCPNGFEGDNCQVNIDECKSNPCGPGKCTDEVNGYHCECPLGLKGITCQEDLDECLSSPCYSGVFCTNTYGSYECGQCPDGYQGDGKICTQILLTTQMPLMVLTDIFTKEAGKKELYFPGGSALKSVGQSSFAVPSPTVYSRQTDSRSFQTTRSLSSSLTTSTARRTTISKLLNGRRTTSNLSRSALFASHAIKPSSRGNSTNIYPTAPTVTNSSKHHSVTSGSPAKNKTDEWPFTKHTDNYLPTLNGTDQAVTMASVERTCADSPCYPGVICEPTISGGFKCGRCPFGYYGDGVTCKAICRHPCGKNMVCAAPNVCRCRNGYSGYNCQTAVCRPDCKNRGKCVRPNVCVCAPGYGGETCEEAYCDQPCENGGTCQARNVCTCPFGYVGPRCETMVCNRHCENGGECVAPDVCKCKPGWYGPTCSTAMCHPVCLNGGTCMKSNICLCPKGFYGAQCQNAVCSPPCKNGGQCMRNNICTCPDGYTGIRCQKSVCNPMCMNGGKCVGPNICSCPSGWKGKQCNTPICLEKCKNGGECVGPNTCHCPAEWEGSQCQTAVCNYKCLYGGRCVLPNVCSCRAGYTGTTCNQRVQGFRG

Target ORF information:

RefSeq Version	XM_002939011.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939011.4

ORF Insert Sequence:

ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC 
CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT 
GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG 
CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA 
CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT 
TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG 
AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC 
TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC 
AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA 
GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC 
ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC 
AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA 
ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA 
CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC 
CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA 
GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA 
TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT 
CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT 
TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT 
CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA 
GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT 
GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT 
CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG 
TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC 
GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT 
AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA 
TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC 
TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT 
TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC 
AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA 
TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT 
AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA 
TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA 
ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA 
GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA 
ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT 
CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGATAAAA ACAGATTTAA GCGGCAAGAT 
TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT 
AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG 
CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC 
AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT 
CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG 
GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA 
TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT 
GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC 
TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT 
GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA 
CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA 
GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT 
GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG 
CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT 
GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA 
ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC 
TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT 
CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT 
CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TCACCTTTGA ATCTGGTCCT 
GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG 
CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG 
GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT 
CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC 
CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA 
GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT 
TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA 
TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG 
ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT 
GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT 
TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA 
ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG 
CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT 
GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG 
ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT 
GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG 
GCAGTTACCA TGGCGTCTGT GGAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT 
ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC 
GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC 
GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT 
GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC 
GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT 
GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA 
TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT 
GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA 
GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC 
TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC 
ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT 
GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC 
CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT 
GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG 
TGCCAGACTG CTGTCTGCAA CTACAAATGT TTATATGGAG GCCGCTGTGT ACTGCCCAAT 
GTATGCTCAT GTCGCGCAGG ATACACTGGA ACGACTTGTA ACCAGAGAGT ACAGGGGTTT 
CGTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwde ( XM_012965592.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821046.1 Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X2, mRNA.

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CloneID	OXa36289
Clone ID Related Accession (Same CDS sequence)	XM_012965592.2
Accession Version	XM_012965592.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor D and EGF domain-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC 
CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT 
GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG 
CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA 
CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT 
TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG 
AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC 
TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC 
AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA 
GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC 
ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC 
AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA 
ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA 
CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC 
CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA 
GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA 
TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT 
CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT 
TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT 
CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA 
GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT 
GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT 
CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG 
TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC 
GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT 
AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA 
TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC 
TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT 
TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC 
AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA 
TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT 
AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA 
TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA 
ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA 
GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA 
ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT 
CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGATAAAA ACAGATTTAA GCGGCAAGAT 
TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT 
AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG 
CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC 
AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT 
CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG 
GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA 
TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT 
GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC 
TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT 
GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA 
CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA 
GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT 
GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG 
CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT 
GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA 
ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC 
TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT 
CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT 
CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TCACCTTTGA ATCTGGTCCT 
GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG 
CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG 
GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT 
CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC 
CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA 
GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT 
TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA 
TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG 
ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT 
GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT 
TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA 
ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG 
CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT 
GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG 
ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT 
GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG 
GCAGTTACCA TGGCGTCTGT GGAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT 
ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC 
GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC 
GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT 
GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC 
GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT 
GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA 
TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT 
GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA 
GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC 
TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC 
ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT 
GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC 
CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT 
GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG 
TGCCAGACTG GGGTTTCGTG GTTGAGAGAG GTTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821046.1
CDS	217..5535
Translation	MSLNLQPGCATLWVLLLILQTAAAQQECSSSGHQILQNSYRSLDFDSLKLQQSAIHDVICDHSLSPGWYRFMIFDKPAEMPTKCVEMNHCGTQAPVWLSLRDSETLPPPGEIRHLTACATWQFFFSSTKDCCLFRIPVSVRNCGDFFVYLLQPTQGCMGYCAEVVPEPKSKCDGGEIDTNNECTSKVIAKPVQPEPPSNPEISIEISGDSVLLKCDFKAPITNSTMGFVIGWSRLSAEGAKEELRQEAAVQTFSIIELDGINVRLGDRIYCSVSSFFLEQPDVHSSLIESEEFFTGIKLYPESVTITEDGRQYQLTVESTVPVLCLGAIQLQSECKVTLKFHTVNEAEENRDSDLALSSCQVDLLQSPCQNHSCSQTSLYYTAVTDFMEDGDKLTNIVVDPIVSTNFLWNGYSPQSVQITVKDIPTAYCYSFTDPHVITFDGRSYDNFNTGTFVLYKSASRDFEVHVRQWDCGSLHHPASCNCGFVAKERGDIVAFDMCSGQLHESQPHLSVKSRDKTTTNFKITESYHGRKLTVVFSSGSFIRADVSDWGMSLTLRAPSSDFQNTLGLCGTFDGNADNDFHNSKGDQLEGISNNHLHFINQWKISAGGSLFDKIPQFSQLSQNTQYCNCNPDRLDAFESNKLLNPQTDMFSCHGNDNVRFHALIPGLDITSEYINALDTIRDVSKRSVADSLVYGPLPAAVEQHENIRTVSITEQTDGRIKNTISTKNNGDTKTQGNTNHINRFSPEKKADKNRFKRQDYQYISTFPYQSLSQTDLEGFSYFFPEDHSTDVPQKFLPSWPTPSGLSHLYVSEFCHQSVFNSTIGKICNDYLKKHIKDVVQMCIVDVLLKDDLSWAEAGVALLENECERNVLELGLHREEFFKQPINDILVALTCPNHCSGNGQCMDWGCACFEGFSSYDCSILEDQVPEILELENKGLCDIRKYACTSVRAFGLGFKETPTLKCEFTKQQYSGGKWIFAEPVLTDAAFRNPRTVDCQLPVDEQQSDGMEPVDDKPIAKWQIKVSNDGYMFSNAKTMTLYDGVCQNCDALSDVLCTLKEKTCNIDGLCYGEGDPNPTSPCLLCRPDVSKLTWSIAENNQPPVIDSFPDKLQTFYGENFVYQFVGADPEGSAILFTFESGPENSSLSPAGLLIWKVTSTVTQEFVFSLTDDCNAQTQVIVEVIVKPCGCINGGSCVTNINFPPGQGEYLCLCPNGFEGDNCQVNIDECKSNPCGPGKCTDEVNGYHCECPLGLKGITCQEDLDECLSSPCYSGVFCTNTYGSYECGQCPDGYQGDGKICTQILLTTQMPLMVLTDIFTKEAGKKELYFPGGSALKSVGQSSFAVPSPTVYSRQTDSRSFQTTRSLSSSLTTSTARRTTISKLLNGRRTTSNLSRSALFASHAIKPSSRGNSTNIYPTAPTVTNSSKHHSVTSGSPAKNKTDEWPFTKHTDNYLPTLNGTDQAVTMASVERTCADSPCYPGVICEPTISGGFKCGRCPFGYYGDGVTCKAICRHPCGKNMVCAAPNVCRCRNGYSGYNCQTAVCRPDCKNRGKCVRPNVCVCAPGYGGETCEEAYCDQPCENGGTCQARNVCTCPFGYVGPRCETMVCNRHCENGGECVAPDVCKCKPGWYGPTCSTAMCHPVCLNGGTCMKSNICLCPKGFYGAQCQNAVCSPPCKNGGQCMRNNICTCPDGYTGIRCQKSVCNPMCMNGGKCVGPNICSCPSGWKGKQCNTPICLEKCKNGGECVGPNTCHCPAEWEGSQCQTGVSWLREVS

Target ORF information:

RefSeq Version	XM_012965592.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965592.2

ORF Insert Sequence:

ATGTCACTGA ACTTACAGCC GGGATGCGCC ACACTCTGGG TGCTGCTACT TATTCTGCAA 
ACAGCCGCTG CTCAGCAAGA ATGTTCCTCA AGTGGGCATC AGATTCTGCA GAATTCATAC 
CGAAGTCTGG ATTTTGACTC CCTGAAGCTG CAGCAGTCAG CCATCCATGA TGTGATCTGT 
GACCACTCCT TGTCTCCTGG ATGGTATCGC TTCATGATCT TCGATAAGCC GGCTGAGATG 
CCAACCAAGT GCGTGGAGAT GAATCACTGC GGAACACAAG CCCCTGTGTG GCTGTCATTA 
CGGGACTCTG AGACTCTGCC CCCACCTGGG GAGATCCGAC ATCTCACCGC CTGTGCGACT 
TGGCAGTTTT TCTTCAGTAG CACAAAAGAT TGCTGTCTCT TCCGCATCCC TGTGTCAGTG 
AGAAACTGCG GGGATTTCTT TGTATACCTG TTGCAACCTA CTCAGGGGTG CATGGGCTAC 
TGTGCAGAAG TTGTCCCTGA GCCTAAATCA AAGTGTGATG GAGGAGAAAT AGACACCAAC 
AATGAATGTA CAAGTAAAGT AATCGCCAAA CCTGTGCAGC CGGAACCTCC AAGTAATCCA 
GAAATTTCCA TTGAGATATC AGGAGACAGT GTACTTTTAA AATGTGACTT CAAAGCTCCC 
ATTACAAACA GCACCATGGG ATTTGTGATA GGATGGTCAA GACTCTCAGC AGAAGGTGCC 
AAAGAGGAAC TGAGGCAAGA GGCTGCAGTG CAGACATTTT CCATTATAGA ACTGGATGGA 
ATTAATGTCA GACTTGGAGA CCGAATATAT TGCAGTGTTT CAAGCTTTTT TCTGGAACAA 
CCAGATGTTC ACAGCTCCCT AATAGAAAGC GAGGAATTTT TTACTGGCAT AAAGCTATAC 
CCCGAGTCTG TCACCATAAC GGAAGATGGC AGGCAATACC AGCTGACAGT AGAGAGCACA 
GTGCCAGTTC TGTGCTTAGG GGCCATTCAG CTGCAGAGTG AATGTAAAGT CACATTGAAA 
TTCCACACTG TGAATGAAGC TGAGGAAAAT CGTGACTCAG ATTTGGCTCT TTCTTCTTGT 
CAAGTGGACC TCCTACAATC TCCGTGCCAA AACCATTCCT GCAGCCAGAC TTCTCTTTAT 
TACACAGCAG TTACAGATTT CATGGAGGAT GGGGATAAAC TCACAAACAT TGTTGTAGAT 
CCTATTGTGA GCACTAACTT CCTATGGAAT GGGTATTCTC CGCAGAGTGT GCAGATTACA 
GTAAAGGATA TCCCGACTGC GTACTGCTAT TCCTTTACTG ATCCACATGT AATCACATTT 
GATGGAAGAT CATATGACAA TTTTAATACA GGAACCTTTG TGCTGTACAA AAGCGCATCT 
CGTGATTTTG AAGTCCATGT GCGCCAGTGG GACTGTGGGA GCCTTCATCA CCCAGCCTCG 
TGCAACTGTG GCTTTGTTGC TAAAGAAAGA GGTGACATTG TTGCCTTTGA TATGTGCAGC 
GGTCAGCTTC ACGAGTCCCA GCCGCATTTG TCAGTAAAGA GTCGAGATAA AACAACGACT 
AATTTTAAAA TTACAGAATC TTATCATGGA AGGAAATTAA CAGTGGTTTT CTCCTCTGGA 
TCTTTTATCC GAGCTGATGT GAGTGACTGG GGAATGAGTT TAACTCTCAG GGCACCAAGC 
TCAGATTTTC AGAACACATT GGGACTTTGT GGCACCTTTG ATGGAAATGC AGACAATGAT 
TTTCATAACA GCAAAGGAGA TCAACTAGAA GGGATTTCAA ACAACCACCT TCATTTTATC 
AACCAGTGGA AAATTTCCGC TGGAGGAAGT TTATTTGATA AAATCCCACA ATTTTCCCAA 
TTGTCACAAA ACACTCAGTA TTGCAACTGC AATCCAGACA GATTGGATGC ATTTGAGTCT 
AACAAACTTC TAAATCCTCA GACTGATATG TTTTCTTGCC ATGGAAATGA TAACGTTCGA 
TTCCATGCAC TCATACCAGG TTTGGATATC ACATCTGAAT ACATAAATGC CCTTGATACA 
ATCCGAGACG TAAGCAAGCG TTCTGTTGCT GATAGCCTTG TCTACGGACC ATTACCTGCA 
GCAGTTGAAC AGCATGAAAA TATCAGAACT GTTAGCATTA CTGAGCAAAC TGATGGAAGA 
ATAAAAAACA CAATCTCCAC AAAAAATAAT GGAGATACAA AAACCCAAGG CAATACAAAT 
CACATCAATA GATTTAGTCC TGAAAAGAAA GCTGATAAAA ACAGATTTAA GCGGCAAGAT 
TATCAATACA TTTCAACATT TCCATATCAA AGCCTTAGCC AAACAGACTT GGAAGGTTTT 
AGCTACTTTT TTCCTGAAGA CCACTCCACA GATGTTCCTC AGAAATTTTT GCCTTCTTGG 
CCAACACCGT CAGGCCTGAG TCATTTGTAT GTTTCCGAAT TTTGCCATCA ATCGGTTTTC 
AACTCAACCA TTGGAAAAAT ATGCAATGAT TATCTAAAGA AACATATCAA AGATGTAGTT 
CAGATGTGCA TTGTGGACGT ACTGTTAAAG GATGACCTGA GCTGGGCAGA GGCTGGAGTG 
GCCCTGCTGG AAAATGAATG TGAAAGAAAT GTTCTAGAAT TAGGACTTCA CAGAGAAGAA 
TTCTTCAAAC AACCCATCAA TGATATTTTG GTAGCCCTGA CATGCCCTAA TCACTGCAGT 
GGTAATGGAC AATGTATGGA CTGGGGATGT GCTTGCTTCG AAGGTTTCAG TTCCTACGAC 
TGTAGCATTT TGGAAGATCA GGTGCCTGAG ATTTTGGAAC TTGAAAATAA GGGATTGTGT 
GACATTCGAA AATATGCCTG CACTTCTGTA AGGGCATTCG GTCTTGGCTT TAAAGAAACA 
CCGACCCTGA AGTGTGAATT TACTAAGCAG CAGTATAGTG GTGGAAAGTG GATTTTTGCA 
GAGCCAGTGC TAACAGATGC TGCCTTCAGG AATCCTAGAA CTGTAGACTG TCAATTGCCT 
GTAGATGAGC AACAGTCTGA TGGCATGGAG CCAGTGGATG ATAAACCTAT AGCTAAATGG 
CAAATTAAGG TATCTAATGA TGGTTATATG TTTAGTAATG CTAAAACAAT GACCTTATAT 
GATGGAGTAT GCCAGAATTG TGATGCGCTG TCAGATGTGC TGTGTACATT AAAGGAGAAA 
ACATGTAATA TAGATGGACT CTGCTACGGA GAGGGTGATC CCAATCCTAC AAGCCCATGC 
TTACTGTGCA GACCAGATGT GTCAAAGCTA ACATGGTCTA TTGCTGAAAA TAACCAGCCT 
CCTGTGATTG ATTCTTTCCC AGATAAATTA CAAACTTTCT ATGGGGAAAA CTTTGTGTAT 
CAATTTGTGG GAGCAGATCC AGAAGGTTCT GCCATCCTTT TCACCTTTGA ATCTGGTCCT 
GAAAATTCCA GCTTATCCCC CGCAGGGTTG CTCATATGGA AAGTTACGTC GACGGTCACG 
CAGGAATTTG TGTTTTCTCT AACAGATGAC TGCAATGCTC AGACTCAAGT AATTGTGGAG 
GTCATAGTAA AACCCTGTGG CTGCATTAAT GGCGGCTCAT GTGTAACCAA CATTAACTTT 
CCACCTGGAC AAGGAGAATA TCTGTGTCTT TGCCCAAATG GGTTTGAGGG GGATAACTGC 
CAAGTAAACA TAGATGAATG TAAGTCTAAT CCATGCGGTC CTGGTAAATG TACCGATGAA 
GTGAACGGCT ACCATTGTGA ATGTCCTTTG GGGCTAAAAG GAATAACATG CCAAGAAGAT 
TTGGATGAGT GTTTATCCAG CCCTTGTTAC TCTGGTGTAT TTTGCACAAA TACTTATGGA 
TCATATGAAT GTGGGCAATG CCCAGATGGC TATCAAGGAG ATGGAAAGAT ATGTACACAG 
ATATTATTGA CAACACAGAT GCCTCTAATG GTGTTAACGG ATATCTTTAC CAAAGAAGCT 
GGGAAAAAAG AACTTTACTT CCCTGGAGGT TCAGCATTAA AGTCAGTAGG CCAGTCAAGT 
TTTGCTGTTC CAAGTCCAAC AGTATATAGC AGGCAAACAG ACAGCCGCTC CTTCCAAACA 
ACTCGGAGTC TTTCATCAAG CTTAACAACC AGTACCGCAA GAAGAACGAC TATTTCTAAG 
CTACTAAATG GTCGAAGGAC CACCTCCAAC CTATCTCGCT CAGCTTTGTT TGCCAGTCAT 
GCAATAAAGC CAAGTTCCAG AGGGAACAGT ACTAATATAT ATCCTACTGC ACCAACAGTG 
ACTAACAGCT CAAAGCATCA CTCTGTTACT TCAGGAAGCC CAGCAAAGAA CAAGACTGAT 
GAATGGCCAT TTACAAAACA TACAGATAAC TATTTACCGA CATTGAATGG AACAGATCAG 
GCAGTTACCA TGGCGTCTGT GGAACGGACA TGTGCTGATT CACCCTGTTA TCCTGGAGTT 
ATTTGTGAAC CAACCATCAG CGGAGGCTTT AAATGTGGAC GATGTCCTTT TGGCTACTAC 
GGAGATGGTG TAACATGCAA GGCAATTTGC CGGCATCCAT GTGGAAAGAA TATGGTATGC 
GCTGCTCCAA ATGTTTGCAG ATGTAGAAAT GGCTACTCTG GATACAACTG CCAGACTGCT 
GTGTGTCGTC CTGACTGTAA GAATCGTGGG AAATGTGTGA GACCCAATGT TTGTGTATGC 
GCCCCAGGAT ATGGTGGAGA AACATGTGAA GAAGCCTACT GTGACCAACC ATGTGAAAAT 
GGAGGGACCT GCCAGGCAAG AAATGTCTGC ACCTGCCCAT TTGGTTATGT TGGACCCAGA 
TGTGAAACAA TGGTCTGTAA TCGGCACTGT GAAAATGGTG GCGAGTGTGT CGCTCCAGAT 
GTGTGCAAGT GCAAACCTGG GTGGTACGGC CCAACATGCA GCACAGCCAT GTGTCATCCA 
GTTTGTCTTA ATGGCGGCAC TTGCATGAAA TCCAATATTT GTCTGTGTCC CAAAGGATTC 
TATGGTGCCC AGTGTCAGAA TGCGGTCTGT AGCCCTCCTT GCAAAAATGG CGGCCAGTGC 
ATGAGAAATA ACATTTGCAC GTGCCCAGAT GGATACACTG GAATAAGATG CCAGAAAAGT 
GTCTGCAATC CAATGTGCAT GAATGGAGGG AAATGTGTGG GGCCCAATAT TTGTTCTTGC 
CCATCAGGAT GGAAAGGGAA GCAATGCAAT ACACCTATAT GCCTTGAAAA ATGTAAGAAT 
GGAGGTGAGT GTGTGGGACC CAATACCTGC CACTGTCCAG CAGAGTGGGA AGGAAGCCAG 
TGCCAGACTG GGGTTTCGTG GTTGAGAGAG GTTTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vwde ( XM_012965594.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821048.1 Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X3, mRNA.

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CloneID	OXa36290
Clone ID Related Accession (Same CDS sequence)	XM_012965594.2
Accession Version	XM_012965594.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	von Willebrand factor D and EGF domain-containing protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965594.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCACT GCGGAACACA AGCCCCTGTG TGGCTGTCAT TACGGGACTC TGAGACTCTG 
CCCCCACCTG GGGAGATCCG ACATCTCACC GCCTGTGCGA CTTGGCAGTT TTTCTTCAGT 
AGCACAAAAG ATTGCTGTCT CTTCCGCATC CCTGTGTCAG TGAGAAACTG CGGGGATTTC 
TTTGTATACC TGTTGCAACC TACTCAGGGG TGCATGGGCT ACTGTGCAGA AGTTGTCCCT 
GAGCCTAAAT CAAAGTGTGA TGGAGGAGAA ATAGACACCA ACAATGAATG TACAAGTAAA 
GTAATCGCCA AACCTGTGCA GCCGGAACCT CCAAGTAATC CAGAAATTTC CATTGAGATA 
TCAGGAGACA GTGTACTTTT AAAATGTGAC TTCAAAGCTC CCATTACAAA CAGCACCATG 
GGATTTGTGA TAGGATGGTC AAGACTCTCA GCAGAAGGTG CCAAAGAGGA ACTGAGGCAA 
GAGGCTGCAG TGCAGACATT TTCCATTATA GAACTGGATG GAATTAATGT CAGACTTGGA 
GACCGAATAT ATTGCAGTGT TTCAAGCTTT TTTCTGGAAC AACCAGATGT TCACAGCTCC 
CTAATAGAAA GCGAGGAATT TTTTACTGGC ATAAAGCTAT ACCCCGAGTC TGTCACCATA 
ACGGAAGATG GCAGGCAATA CCAGCTGACA GTAGAGAGCA CAGTGCCAGT TCTGTGCTTA 
GGGGCCATTC AGCTGCAGAG TGAATGTAAA GTCACATTGA AATTCCACAC TGTGAATGAA 
GCTGAGGAAA ATCGTGACTC AGATTTGGCT CTTTCTTCTT GTCAAGTGGA CCTCCTACAA 
TCTCCGTGCC AAAACCATTC CTGCAGCCAG ACTTCTCTTT ATTACACAGC AGTTACAGAT 
TTCATGGAGG ATGGGGATAA ACTCACAAAC ATTGTTGTAG ATCCTATTGT GAGCACTAAC 
TTCCTATGGA ATGGGTATTC TCCGCAGAGT GTGCAGATTA CAGTAAAGGA TATCCCGACT 
GCGTACTGCT ATTCCTTTAC TGATCCACAT GTAATCACAT TTGATGGAAG ATCATATGAC 
AATTTTAATA CAGGAACCTT TGTGCTGTAC AAAAGCGCAT CTCGTGATTT TGAAGTCCAT 
GTGCGCCAGT GGGACTGTGG GAGCCTTCAT CACCCAGCCT CGTGCAACTG TGGCTTTGTT 
GCTAAAGAAA GAGGTGACAT TGTTGCCTTT GATATGTGCA GCGGTCAGCT TCACGAGTCC 
CAGCCGCATT TGTCAGTAAA GAGTCGAGAT AAAACAACGA CTAATTTTAA AATTACAGAA 
TCTTATCATG GAAGGAAATT AACAGTGGTT TTCTCCTCTG GATCTTTTAT CCGAGCTGAT 
GTGAGTGACT GGGGAATGAG TTTAACTCTC AGGGCACCAA GCTCAGATTT TCAGAACACA 
TTGGGACTTT GTGGCACCTT TGATGGAAAT GCAGACAATG ATTTTCATAA CAGCAAAGGA 
GATCAACTAG AAGGGATTTC AAACAACCAC CTTCATTTTA TCAACCAGTG GAAAATTTCC 
GCTGGAGGAA GTTTATTTGA TAAAATCCCA CAATTTTCCC AATTGTCACA AAACACTCAG 
TATTGCAACT GCAATCCAGA CAGATTGGAT GCATTTGAGT CTAACAAACT TCTAAATCCT 
CAGACTGATA TGTTTTCTTG CCATGGAAAT GATAACGTTC GATTCCATGC ACTCATACCA 
GGTTTGGATA TCACATCTGA ATACATAAAT GCCCTTGATA CAATCCGAGA CGTAAGCAAG 
CGTTCTGTTG CTGATAGCCT TGTCTACGGA CCATTACCTG CAGCAGTTGA ACAGCATGAA 
AATATCAGAA CTGTTAGCAT TACTGAGCAA ACTGATGGAA GAATAAAAAA CACAATCTCC 
ACAAAAAATA ATGGAGATAC AAAAACCCAA GGCAATACAA ATCACATCAA TAGATTTAGT 
CCTGAAAAGA AAGCTGATAA AAACAGATTT AAGCGGCAAG ATTATCAATA CATTTCAACA 
TTTCCATATC AAAGCCTTAG CCAAACAGAC TTGGAAGGTT TTAGCTACTT TTTTCCTGAA 
GACCACTCCA CAGATGTTCC TCAGAAATTT TTGCCTTCTT GGCCAACACC GTCAGGCCTG 
AGTCATTTGT ATGTTTCCGA ATTTTGCCAT CAATCGGTTT TCAACTCAAC CATTGGAAAA 
ATATGCAATG ATTATCTAAA GAAACATATC AAAGATGTAG TTCAGATGTG CATTGTGGAC 
GTACTGTTAA AGGATGACCT GAGCTGGGCA GAGGCTGGAG TGGCCCTGCT GGAAAATGAA 
TGTGAAAGAA ATGTTCTAGA ATTAGGACTT CACAGAGAAG AATTCTTCAA ACAACCCATC 
AATGATATTT TGGTAGCCCT GACATGCCCT AATCACTGCA GTGGTAATGG ACAATGTATG 
GACTGGGGAT GTGCTTGCTT CGAAGGTTTC AGTTCCTACG ACTGTAGCAT TTTGGAAGAT 
CAGGTGCCTG AGATTTTGGA ACTTGAAAAT AAGGGATTGT GTGACATTCG AAAATATGCC 
TGCACTTCTG TAAGGGCATT CGGTCTTGGC TTTAAAGAAA CACCGACCCT GAAGTGTGAA 
TTTACTAAGC AGCAGTATAG TGGTGGAAAG TGGATTTTTG CAGAGCCAGT GCTAACAGAT 
GCTGCCTTCA GGAATCCTAG AACTGTAGAC TGTCAATTGC CTGTAGATGA GCAACAGTCT 
GATGGCATGG AGCCAGTGGA TGATAAACCT ATAGCTAAAT GGCAAATTAA GGTATCTAAT 
GATGGTTATA TGTTTAGTAA TGCTAAAACA ATGACCTTAT ATGATGGAGT ATGCCAGAAT 
TGTGATGCGC TGTCAGATGT GCTGTGTACA TTAAAGGAGA AAACATGTAA TATAGATGGA 
CTCTGCTACG GAGAGGGTGA TCCCAATCCT ACAAGCCCAT GCTTACTGTG CAGACCAGAT 
GTGTCAAAGC TAACATGGTC TATTGCTGAA AATAACCAGC CTCCTGTGAT TGATTCTTTC 
CCAGATAAAT TACAAACTTT CTATGGGGAA AACTTTGTGT ATCAATTTGT GGGAGCAGAT 
CCAGAAGGTT CTGCCATCCT TTTCACCTTT GAATCTGGTC CTGAAAATTC CAGCTTATCC 
CCCGCAGGGT TGCTCATATG GAAAGTTACG TCGACGGTCA CGCAGGAATT TGTGTTTTCT 
CTAACAGATG ACTGCAATGC TCAGACTCAA GTAATTGTGG AGGTCATAGT AAAACCCTGT 
GGCTGCATTA ATGGCGGCTC ATGTGTAACC AACATTAACT TTCCACCTGG ACAAGGAGAA 
TATCTGTGTC TTTGCCCAAA TGGGTTTGAG GGGGATAACT GCCAAGTAAA CATAGATGAA 
TGTAAGTCTA ATCCATGCGG TCCTGGTAAA TGTACCGATG AAGTGAACGG CTACCATTGT 
GAATGTCCTT TGGGGCTAAA AGGAATAACA TGCCAAGAAG ATTTGGATGA GTGTTTATCC 
AGCCCTTGTT ACTCTGGTGT ATTTTGCACA AATACTTATG GATCATATGA ATGTGGGCAA 
TGCCCAGATG GCTATCAAGG AGATGGAAAG ATATGTACAC AGATATTATT GACAACACAG 
ATGCCTCTAA TGGTGTTAAC GGATATCTTT ACCAAAGAAG CTGGGAAAAA AGAACTTTAC 
TTCCCTGGAG GTTCAGCATT AAAGTCAGTA GGCCAGTCAA GTTTTGCTGT TCCAAGTCCA 
ACAGTATATA GCAGGCAAAC AGACAGCCGC TCCTTCCAAA CAACTCGGAG TCTTTCATCA 
AGCTTAACAA CCAGTACCGC AAGAAGAACG ACTATTTCTA AGCTACTAAA TGGTCGAAGG 
ACCACCTCCA ACCTATCTCG CTCAGCTTTG TTTGCCAGTC ATGCAATAAA GCCAAGTTCC 
AGAGGGAACA GTACTAATAT ATATCCTACT GCACCAACAG TGACTAACAG CTCAAAGCAT 
CACTCTGTTA CTTCAGGAAG CCCAGCAAAG AACAAGACTG ATGAATGGCC ATTTACAAAA 
CATACAGATA ACTATTTACC GACATTGAAT GGAACAGATC AGGCAGTTAC CATGGCGTCT 
GTGGAACGGA CATGTGCTGA TTCACCCTGT TATCCTGGAG TTATTTGTGA ACCAACCATC 
AGCGGAGGCT TTAAATGTGG ACGATGTCCT TTTGGCTACT ACGGAGATGG TGTAACATGC 
AAGGCAATTT GCCGGCATCC ATGTGGAAAG AATATGGTAT GCGCTGCTCC AAATGTTTGC 
AGATGTAGAA ATGGCTACTC TGGATACAAC TGCCAGACTG CTGTGTGTCG TCCTGACTGT 
AAGAATCGTG GGAAATGTGT GAGACCCAAT GTTTGTGTAT GCGCCCCAGG ATATGGTGGA 
GAAACATGTG AAGAAGCCTA CTGTGACCAA CCATGTGAAA ATGGAGGGAC CTGCCAGGCA 
AGAAATGTCT GCACCTGCCC ATTTGGTTAT GTTGGACCCA GATGTGAAAC AATGGTCTGT 
AATCGGCACT GTGAAAATGG TGGCGAGTGT GTCGCTCCAG ATGTGTGCAA GTGCAAACCT 
GGGTGGTACG GCCCAACATG CAGCACAGCC ATGTGTCATC CAGTTTGTCT TAATGGCGGC 
ACTTGCATGA AATCCAATAT TTGTCTGTGT CCCAAAGGAT TCTATGGTGC CCAGTGTCAG 
AATGCGGTCT GTAGCCCTCC TTGCAAAAAT GGCGGCCAGT GCATGAGAAA TAACATTTGC 
ACGTGCCCAG ATGGATACAC TGGAATAAGA TGCCAGAAAA GTGTCTGCAA TCCAATGTGC 
ATGAATGGAG GGAAATGTGT GGGGCCCAAT ATTTGTTCTT GCCCATCAGG ATGGAAAGGG 
AAGCAATGCA ATACACCTAT ATGCCTTGAA AAATGTAAGA ATGGAGGTGA GTGTGTGGGA 
CCCAATACCT GCCACTGTCC AGCAGAGTGG GAAGGAAGCC AGTGCCAGAC TGCTGTCTGC 
AACTACAAAT GTTTATATGG AGGCCGCTGT GTACTGCCCA ATGTATGCTC ATGTCGCGCA 
GGATACACTG GAACGACTTG TAACCAGAGA GTACAGGGGT TTCGTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821048.1
CDS	90..5240
Translation	MNHCGTQAPVWLSLRDSETLPPPGEIRHLTACATWQFFFSSTKDCCLFRIPVSVRNCGDFFVYLLQPTQGCMGYCAEVVPEPKSKCDGGEIDTNNECTSKVIAKPVQPEPPSNPEISIEISGDSVLLKCDFKAPITNSTMGFVIGWSRLSAEGAKEELRQEAAVQTFSIIELDGINVRLGDRIYCSVSSFFLEQPDVHSSLIESEEFFTGIKLYPESVTITEDGRQYQLTVESTVPVLCLGAIQLQSECKVTLKFHTVNEAEENRDSDLALSSCQVDLLQSPCQNHSCSQTSLYYTAVTDFMEDGDKLTNIVVDPIVSTNFLWNGYSPQSVQITVKDIPTAYCYSFTDPHVITFDGRSYDNFNTGTFVLYKSASRDFEVHVRQWDCGSLHHPASCNCGFVAKERGDIVAFDMCSGQLHESQPHLSVKSRDKTTTNFKITESYHGRKLTVVFSSGSFIRADVSDWGMSLTLRAPSSDFQNTLGLCGTFDGNADNDFHNSKGDQLEGISNNHLHFINQWKISAGGSLFDKIPQFSQLSQNTQYCNCNPDRLDAFESNKLLNPQTDMFSCHGNDNVRFHALIPGLDITSEYINALDTIRDVSKRSVADSLVYGPLPAAVEQHENIRTVSITEQTDGRIKNTISTKNNGDTKTQGNTNHINRFSPEKKADKNRFKRQDYQYISTFPYQSLSQTDLEGFSYFFPEDHSTDVPQKFLPSWPTPSGLSHLYVSEFCHQSVFNSTIGKICNDYLKKHIKDVVQMCIVDVLLKDDLSWAEAGVALLENECERNVLELGLHREEFFKQPINDILVALTCPNHCSGNGQCMDWGCACFEGFSSYDCSILEDQVPEILELENKGLCDIRKYACTSVRAFGLGFKETPTLKCEFTKQQYSGGKWIFAEPVLTDAAFRNPRTVDCQLPVDEQQSDGMEPVDDKPIAKWQIKVSNDGYMFSNAKTMTLYDGVCQNCDALSDVLCTLKEKTCNIDGLCYGEGDPNPTSPCLLCRPDVSKLTWSIAENNQPPVIDSFPDKLQTFYGENFVYQFVGADPEGSAILFTFESGPENSSLSPAGLLIWKVTSTVTQEFVFSLTDDCNAQTQVIVEVIVKPCGCINGGSCVTNINFPPGQGEYLCLCPNGFEGDNCQVNIDECKSNPCGPGKCTDEVNGYHCECPLGLKGITCQEDLDECLSSPCYSGVFCTNTYGSYECGQCPDGYQGDGKICTQILLTTQMPLMVLTDIFTKEAGKKELYFPGGSALKSVGQSSFAVPSPTVYSRQTDSRSFQTTRSLSSSLTTSTARRTTISKLLNGRRTTSNLSRSALFASHAIKPSSRGNSTNIYPTAPTVTNSSKHHSVTSGSPAKNKTDEWPFTKHTDNYLPTLNGTDQAVTMASVERTCADSPCYPGVICEPTISGGFKCGRCPFGYYGDGVTCKAICRHPCGKNMVCAAPNVCRCRNGYSGYNCQTAVCRPDCKNRGKCVRPNVCVCAPGYGGETCEEAYCDQPCENGGTCQARNVCTCPFGYVGPRCETMVCNRHCENGGECVAPDVCKCKPGWYGPTCSTAMCHPVCLNGGTCMKSNICLCPKGFYGAQCQNAVCSPPCKNGGQCMRNNICTCPDGYTGIRCQKSVCNPMCMNGGKCVGPNICSCPSGWKGKQCNTPICLEKCKNGGECVGPNTCHCPAEWEGSQCQTAVCNYKCLYGGRCVLPNVCSCRAGYTGTTCNQRVQGFRG

Target ORF information:

RefSeq Version	XM_012965594.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis von Willebrand factor D and EGF domains (vwde), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965594.2

ORF Insert Sequence:

ATGAATCACT GCGGAACACA AGCCCCTGTG TGGCTGTCAT TACGGGACTC TGAGACTCTG 
CCCCCACCTG GGGAGATCCG ACATCTCACC GCCTGTGCGA CTTGGCAGTT TTTCTTCAGT 
AGCACAAAAG ATTGCTGTCT CTTCCGCATC CCTGTGTCAG TGAGAAACTG CGGGGATTTC 
TTTGTATACC TGTTGCAACC TACTCAGGGG TGCATGGGCT ACTGTGCAGA AGTTGTCCCT 
GAGCCTAAAT CAAAGTGTGA TGGAGGAGAA ATAGACACCA ACAATGAATG TACAAGTAAA 
GTAATCGCCA AACCTGTGCA GCCGGAACCT CCAAGTAATC CAGAAATTTC CATTGAGATA 
TCAGGAGACA GTGTACTTTT AAAATGTGAC TTCAAAGCTC CCATTACAAA CAGCACCATG 
GGATTTGTGA TAGGATGGTC AAGACTCTCA GCAGAAGGTG CCAAAGAGGA ACTGAGGCAA 
GAGGCTGCAG TGCAGACATT TTCCATTATA GAACTGGATG GAATTAATGT CAGACTTGGA 
GACCGAATAT ATTGCAGTGT TTCAAGCTTT TTTCTGGAAC AACCAGATGT TCACAGCTCC 
CTAATAGAAA GCGAGGAATT TTTTACTGGC ATAAAGCTAT ACCCCGAGTC TGTCACCATA 
ACGGAAGATG GCAGGCAATA CCAGCTGACA GTAGAGAGCA CAGTGCCAGT TCTGTGCTTA 
GGGGCCATTC AGCTGCAGAG TGAATGTAAA GTCACATTGA AATTCCACAC TGTGAATGAA 
GCTGAGGAAA ATCGTGACTC AGATTTGGCT CTTTCTTCTT GTCAAGTGGA CCTCCTACAA 
TCTCCGTGCC AAAACCATTC CTGCAGCCAG ACTTCTCTTT ATTACACAGC AGTTACAGAT 
TTCATGGAGG ATGGGGATAA ACTCACAAAC ATTGTTGTAG ATCCTATTGT GAGCACTAAC 
TTCCTATGGA ATGGGTATTC TCCGCAGAGT GTGCAGATTA CAGTAAAGGA TATCCCGACT 
GCGTACTGCT ATTCCTTTAC TGATCCACAT GTAATCACAT TTGATGGAAG ATCATATGAC 
AATTTTAATA CAGGAACCTT TGTGCTGTAC AAAAGCGCAT CTCGTGATTT TGAAGTCCAT 
GTGCGCCAGT GGGACTGTGG GAGCCTTCAT CACCCAGCCT CGTGCAACTG TGGCTTTGTT 
GCTAAAGAAA GAGGTGACAT TGTTGCCTTT GATATGTGCA GCGGTCAGCT TCACGAGTCC 
CAGCCGCATT TGTCAGTAAA GAGTCGAGAT AAAACAACGA CTAATTTTAA AATTACAGAA 
TCTTATCATG GAAGGAAATT AACAGTGGTT TTCTCCTCTG GATCTTTTAT CCGAGCTGAT 
GTGAGTGACT GGGGAATGAG TTTAACTCTC AGGGCACCAA GCTCAGATTT TCAGAACACA 
TTGGGACTTT GTGGCACCTT TGATGGAAAT GCAGACAATG ATTTTCATAA CAGCAAAGGA 
GATCAACTAG AAGGGATTTC AAACAACCAC CTTCATTTTA TCAACCAGTG GAAAATTTCC 
GCTGGAGGAA GTTTATTTGA TAAAATCCCA CAATTTTCCC AATTGTCACA AAACACTCAG 
TATTGCAACT GCAATCCAGA CAGATTGGAT GCATTTGAGT CTAACAAACT TCTAAATCCT 
CAGACTGATA TGTTTTCTTG CCATGGAAAT GATAACGTTC GATTCCATGC ACTCATACCA 
GGTTTGGATA TCACATCTGA ATACATAAAT GCCCTTGATA CAATCCGAGA CGTAAGCAAG 
CGTTCTGTTG CTGATAGCCT TGTCTACGGA CCATTACCTG CAGCAGTTGA ACAGCATGAA 
AATATCAGAA CTGTTAGCAT TACTGAGCAA ACTGATGGAA GAATAAAAAA CACAATCTCC 
ACAAAAAATA ATGGAGATAC AAAAACCCAA GGCAATACAA ATCACATCAA TAGATTTAGT 
CCTGAAAAGA AAGCTGATAA AAACAGATTT AAGCGGCAAG ATTATCAATA CATTTCAACA 
TTTCCATATC AAAGCCTTAG CCAAACAGAC TTGGAAGGTT TTAGCTACTT TTTTCCTGAA 
GACCACTCCA CAGATGTTCC TCAGAAATTT TTGCCTTCTT GGCCAACACC GTCAGGCCTG 
AGTCATTTGT ATGTTTCCGA ATTTTGCCAT CAATCGGTTT TCAACTCAAC CATTGGAAAA 
ATATGCAATG ATTATCTAAA GAAACATATC AAAGATGTAG TTCAGATGTG CATTGTGGAC 
GTACTGTTAA AGGATGACCT GAGCTGGGCA GAGGCTGGAG TGGCCCTGCT GGAAAATGAA 
TGTGAAAGAA ATGTTCTAGA ATTAGGACTT CACAGAGAAG AATTCTTCAA ACAACCCATC 
AATGATATTT TGGTAGCCCT GACATGCCCT AATCACTGCA GTGGTAATGG ACAATGTATG 
GACTGGGGAT GTGCTTGCTT CGAAGGTTTC AGTTCCTACG ACTGTAGCAT TTTGGAAGAT 
CAGGTGCCTG AGATTTTGGA ACTTGAAAAT AAGGGATTGT GTGACATTCG AAAATATGCC 
TGCACTTCTG TAAGGGCATT CGGTCTTGGC TTTAAAGAAA CACCGACCCT GAAGTGTGAA 
TTTACTAAGC AGCAGTATAG TGGTGGAAAG TGGATTTTTG CAGAGCCAGT GCTAACAGAT 
GCTGCCTTCA GGAATCCTAG AACTGTAGAC TGTCAATTGC CTGTAGATGA GCAACAGTCT 
GATGGCATGG AGCCAGTGGA TGATAAACCT ATAGCTAAAT GGCAAATTAA GGTATCTAAT 
GATGGTTATA TGTTTAGTAA TGCTAAAACA ATGACCTTAT ATGATGGAGT ATGCCAGAAT 
TGTGATGCGC TGTCAGATGT GCTGTGTACA TTAAAGGAGA AAACATGTAA TATAGATGGA 
CTCTGCTACG GAGAGGGTGA TCCCAATCCT ACAAGCCCAT GCTTACTGTG CAGACCAGAT 
GTGTCAAAGC TAACATGGTC TATTGCTGAA AATAACCAGC CTCCTGTGAT TGATTCTTTC 
CCAGATAAAT TACAAACTTT CTATGGGGAA AACTTTGTGT ATCAATTTGT GGGAGCAGAT 
CCAGAAGGTT CTGCCATCCT TTTCACCTTT GAATCTGGTC CTGAAAATTC CAGCTTATCC 
CCCGCAGGGT TGCTCATATG GAAAGTTACG TCGACGGTCA CGCAGGAATT TGTGTTTTCT 
CTAACAGATG ACTGCAATGC TCAGACTCAA GTAATTGTGG AGGTCATAGT AAAACCCTGT 
GGCTGCATTA ATGGCGGCTC ATGTGTAACC AACATTAACT TTCCACCTGG ACAAGGAGAA 
TATCTGTGTC TTTGCCCAAA TGGGTTTGAG GGGGATAACT GCCAAGTAAA CATAGATGAA 
TGTAAGTCTA ATCCATGCGG TCCTGGTAAA TGTACCGATG AAGTGAACGG CTACCATTGT 
GAATGTCCTT TGGGGCTAAA AGGAATAACA TGCCAAGAAG ATTTGGATGA GTGTTTATCC 
AGCCCTTGTT ACTCTGGTGT ATTTTGCACA AATACTTATG GATCATATGA ATGTGGGCAA 
TGCCCAGATG GCTATCAAGG AGATGGAAAG ATATGTACAC AGATATTATT GACAACACAG 
ATGCCTCTAA TGGTGTTAAC GGATATCTTT ACCAAAGAAG CTGGGAAAAA AGAACTTTAC 
TTCCCTGGAG GTTCAGCATT AAAGTCAGTA GGCCAGTCAA GTTTTGCTGT TCCAAGTCCA 
ACAGTATATA GCAGGCAAAC AGACAGCCGC TCCTTCCAAA CAACTCGGAG TCTTTCATCA 
AGCTTAACAA CCAGTACCGC AAGAAGAACG ACTATTTCTA AGCTACTAAA TGGTCGAAGG 
ACCACCTCCA ACCTATCTCG CTCAGCTTTG TTTGCCAGTC ATGCAATAAA GCCAAGTTCC 
AGAGGGAACA GTACTAATAT ATATCCTACT GCACCAACAG TGACTAACAG CTCAAAGCAT 
CACTCTGTTA CTTCAGGAAG CCCAGCAAAG AACAAGACTG ATGAATGGCC ATTTACAAAA 
CATACAGATA ACTATTTACC GACATTGAAT GGAACAGATC AGGCAGTTAC CATGGCGTCT 
GTGGAACGGA CATGTGCTGA TTCACCCTGT TATCCTGGAG TTATTTGTGA ACCAACCATC 
AGCGGAGGCT TTAAATGTGG ACGATGTCCT TTTGGCTACT ACGGAGATGG TGTAACATGC 
AAGGCAATTT GCCGGCATCC ATGTGGAAAG AATATGGTAT GCGCTGCTCC AAATGTTTGC 
AGATGTAGAA ATGGCTACTC TGGATACAAC TGCCAGACTG CTGTGTGTCG TCCTGACTGT 
AAGAATCGTG GGAAATGTGT GAGACCCAAT GTTTGTGTAT GCGCCCCAGG ATATGGTGGA 
GAAACATGTG AAGAAGCCTA CTGTGACCAA CCATGTGAAA ATGGAGGGAC CTGCCAGGCA 
AGAAATGTCT GCACCTGCCC ATTTGGTTAT GTTGGACCCA GATGTGAAAC AATGGTCTGT 
AATCGGCACT GTGAAAATGG TGGCGAGTGT GTCGCTCCAG ATGTGTGCAA GTGCAAACCT 
GGGTGGTACG GCCCAACATG CAGCACAGCC ATGTGTCATC CAGTTTGTCT TAATGGCGGC 
ACTTGCATGA AATCCAATAT TTGTCTGTGT CCCAAAGGAT TCTATGGTGC CCAGTGTCAG 
AATGCGGTCT GTAGCCCTCC TTGCAAAAAT GGCGGCCAGT GCATGAGAAA TAACATTTGC 
ACGTGCCCAG ATGGATACAC TGGAATAAGA TGCCAGAAAA GTGTCTGCAA TCCAATGTGC 
ATGAATGGAG GGAAATGTGT GGGGCCCAAT ATTTGTTCTT GCCCATCAGG ATGGAAAGGG 
AAGCAATGCA ATACACCTAT ATGCCTTGAA AAATGTAAGA ATGGAGGTGA GTGTGTGGGA 
CCCAATACCT GCCACTGTCC AGCAGAGTGG GAAGGAAGCC AGTGCCAGAC TGCTGTCTGC 
AACTACAAAT GTTTATATGG AGGCCGCTGT GTACTGCCCA ATGTATGCTC ATGTCGCGCA 
GGATACACTG GAACGACTTG TAACCAGAGA GTACAGGGGT TTCGTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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vwde cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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