dhx32 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dhx32
Entrez Gene ID	100495407
Full Name	DEAH-box helicase 32 (putative)
General protein information	Preferred Names DEAH-box helicase 32 (putative) Names putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 DEAH (Asp-Glu-Ala-His) box polypeptide 32
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004912720.3	XP_004912777.1	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
XM_018095827.1	XP_017951316.1	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
XM_004912720.4	XP_004912777.2	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 isoform X1
XM_031906149.1	XP_031762009.1	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 isoform X1
XM_031906150.1	XP_031762010.1	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 isoform X2
XM_012959634.2	XP_012815088.1	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
XM_002932650.5	XP_002932696.3	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 isoform X1
XM_002932650.4	XP_002932696.2	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

Pathways from BioSystems

Homologies

	DHX32	XP_001085971.1
Bos taurus (cattle)	DHX32	NP_001094614.1
Xenopus tropicalis (tropical clawed frog)	dhx32	XP_002932696.2
Canis lupus familiaris (dog)	DHX32	XP_003433686.1
Gallus gallus (chicken)	LOC768812	XP_003641552.1
Danio rerio (zebrafish)	dhx32	XP_005158794.1
Danio rerio (zebrafish)	dhx32a	NP_001189401.1
Homo sapiens (human)	DHX32	NP_060650.2
Mus musculus (house mouse)	Dhx32	NP_001272960.1
Rattus norvegicus (Norway rat)	Dhx32	NP_001123511.1
Pan troglodytes (chimpanzee)	DHX32	XP_001138928.1

Gene Ontology

The following dhx32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dhx32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa07399	XM_018095827.1 Latest version!	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa07399	XM_002932650.4 Latest version!	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa07399	XM_012959634.2 Latest version!	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa07399	XM_004912720.3 Latest version!	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa62622	XM_031906149.1 Latest version!	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa62622	XM_002932650.5 Latest version!	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa62622	XM_004912720.4 Latest version!	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa62623	XM_031906150.1 Latest version!	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dhx32 ( XM_004912720.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912777.1 Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa07399
Clone ID Related Accession (Same CDS sequence)	XM_004912720.3 , XM_018095827.1 , XM_012959634.2 , XM_002932650.4
Accession Version	XM_004912720.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTT CACTAGAGGC CTATGAACAG GACGGCGATG AACTCATTTC CGAAGAGAAA 
AGCTGTTGCC TCGATGTTAC AGACTCAAGT GATGTGGAAG GAGATGAACT TAATGACTTG 
GAACTAAATC CATTTGATGG TTTGCCTTAC TCATCTCGTT TTTACAAACT CTTAAAAGAA 
AGAGAAGATC TTCCCATATG GAAAGCCAAA TATGACTTTT TGGAGCGATT ATCTAACAGC 
CAAGTTGTGG TTGTGTCGGC GGGACCTAAG AGCGGAAAGA GTTCACAAAT TCCTCAATGG 
TGCGCAGAAT ACTGCTTAGC AGGGCACTAT CAACATGGGG CAGTTGTGTG TACGCAGGCG 
CACAAGCAGA CGGCAGTATG GCTGGCCATG AGAGTGGCAG ACGAAATGGA TGTCAATATA 
GGACATGAAG TTGGCTATAT TGTGCCGTTC GAAAACTGCT GTACAAGCGA GACAATTTTA 
AGGTATTGCA CGGACGAGAT GTTGCTGAGG GAAATGATGT CCAGCCCCCT TCTGTCCAGT 
TATGGAGTCG TCATTATCGA TGATGTCTAT GAAAGATCAG TTTCTACTGA TGTGCTGCTC 
AGTTGCCTTA AGGTCATTGC AGTCTCAAGA CCTGAACTGA AAGTTGTGAT AATTACCTGC 
CCTGCTCTGT CTGGGACCTT GGTTTCTTAT TATGGAAATG CTCCACTTGT AGAAACAGAA 
AACATGCACT CTGTAGAGTC GGTTTACACC ACAAGCATTC CAAGAGACTA CTTCCACTCA 
ACTTTACGAC TTCTCTTTGA AATACATCAT ACTAAAGAAC AAGGGGACAT TGTTGTCTTT 
CTGGCCTGTG AAGAAGAGAT AAAAAGGGCA TATGAGCATA TCAAACAGGA AGCATTGCAC 
ATGAATCCAG AATTGGGTGA ACTGGTACCC ATTGCTTTGT ACCCCAATCA AAGCATTTCA 
GACTACATAC CTTATGAGGA ACTGGATGAC AACAGCAAAA GCCCAAAAAG AAAAGTGGTA 
TTGACGACCA GCCTTGGCGA GTCTCTCATC TGGATGAAAA ATATCCTCTT TGTCATTGAT 
GTTGGTATAG AGAGGAGGAA GGTATATAAT ACCAGAATCC GCGCAGAATC ACATGTCACG 
CAACCAATAA GTAAGGTCCG GGCTGAGATG AGGAAGCATA TACTGAGCTC ATATTCTAAA 
GGAAAACTAT TTTGCTTGTA CCCCGAGGAC TTTGCACATG AGATGAAACC ATTCCTGACT 
GCAAAAGTGG AAGAGTGCAA TTTGATTTCA ATGGTTCTGT TTCTGAAGAG AATGGACATT 
GCGGGGCTCG CTCACTGTGA CTTCATCAAC AGACCAGACC CTGAAAGTTT AATGCAAGCA 
CTGGAAGATC TGGATTATTT GGCAGCACTT GACAATGATG GAAATCTTTC TGAATTTGGA 
ATAATTATGT CTGAGTTTCC ACTCGATCCA CAGCTGTCAA AATCCATTTT AGCTGCCTGC 
GAATTCGACT GCGTGGATGA AATGCTCACA CTGGCTGCTA TGGTTACAGC CCCGAATTGT 
TTTATCGATT CATCCCCTGA AGCCGAAGAG CTGATTTTAA TTCGTAAAGG AAAGTTTTCT 
CATCCAGAGG GGGACCACTT CACTCTTATA AATATCTACA ATGAATATGA GCAGATGAAG 
AGAAGCAATG CTAGCCAGTA CGATGTTGAA AAATGGTGTC AGGATCACTG CCTAAGTTTC 
CTGGCAATGG AGATGGCGCG TGCCATAAGA AATGAGCTCG CTGACATTAT GAGGCGCATA 
GAGCTTCCAC TGACAGCGCC TGCATTTGGC TCTGAGGAGA ATGTAATAAA CATTAAAAAG 
TCACTTTTGT CAGGATACTT CATGCAAATT GCTCGTGATG TTGATGGTTT GGGAAACTAC 
ATTATGCTGA CACACAAGCA GGTGGGACAA CTCCATCCTG ATTCTGGCTT CAACGGCGCC 
AAGGTTCCAG AATGGGTTCT CTACCACGAA TTTAGCGTCT CTGAGAGAAG CTGCATCAGG 
ATAGTCTCAG AGATCTCACC AAACATGTTC ATGGAGTTCG TTCCCCCGTA CTACTTCAGC 
AACCTGCCCC CTAGTGAGAC TAAAGAGCTC CTGCAGCAGC ACCAGACTGT GCCCAGTTCC 
CCGGCTGAAG AGCCCGGAGA AGAGGAGCCC CTACCTGATG CCGATGATGA AGGTGCAACA 
GAACAAAGAT GCATTATACA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912777.1
CDS	338..2581
Translation	MALSLEAYEQDGDELISEEKSCCLDVTDSSDVEGDELNDLELNPFDGLPYSSRFYKLLKEREDLPIWKAKYDFLERLSNSQVVVVSAGPKSGKSSQIPQWCAEYCLAGHYQHGAVVCTQAHKQTAVWLAMRVADEMDVNIGHEVGYIVPFENCCTSETILRYCTDEMLLREMMSSPLLSSYGVVIIDDVYERSVSTDVLLSCLKVIAVSRPELKVVIITCPALSGTLVSYYGNAPLVETENMHSVESVYTTSIPRDYFHSTLRLLFEIHHTKEQGDIVVFLACEEEIKRAYEHIKQEALHMNPELGELVPIALYPNQSISDYIPYEELDDNSKSPKRKVVLTTSLGESLIWMKNILFVIDVGIERRKVYNTRIRAESHVTQPISKVRAEMRKHILSSYSKGKLFCLYPEDFAHEMKPFLTAKVEECNLISMVLFLKRMDIAGLAHCDFINRPDPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILAACEFDCVDEMLTLAAMVTAPNCFIDSSPEAEELILIRKGKFSHPEGDHFTLINIYNEYEQMKRSNASQYDVEKWCQDHCLSFLAMEMARAIRNELADIMRRIELPLTAPAFGSEENVINIKKSLLSGYFMQIARDVDGLGNYIMLTHKQVGQLHPDSGFNGAKVPEWVLYHEFSVSERSCIRIVSEISPNMFMEFVPPYYFSNLPPSETKELLQQHQTVPSSPAEEPGEEEPLPDADDEGATEQRCIIQ

Target ORF information:

RefSeq Version	XM_004912720.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912720.3

ORF Insert Sequence:

ATGGCTCTTT CACTAGAGGC CTATGAACAG GACGGCGATG AACTCATTTC CGAAGAGAAA 
AGCTGTTGCC TCGATGTTAC AGACTCAAGT GATGTGGAAG GAGATGAACT TAATGACTTG 
GAACTAAATC CATTTGATGG TTTGCCTTAC TCATCTCGTT TTTACAAACT CTTAAAAGAA 
AGAGAAGATC TTCCCATATG GAAAGCCAAA TATGACTTTT TGGAGCGATT ATCTAACAGC 
CAAGTTGTGG TTGTGTCGGC GGGACCTAAG AGCGGAAAGA GTTCACAAAT TCCTCAATGG 
TGCGCAGAAT ACTGCTTAGC AGGGCACTAT CAACATGGGG CAGTTGTGTG TACGCAGGCG 
CACAAGCAGA CGGCAGTATG GCTGGCCATG AGAGTGGCAG ACGAAATGGA TGTCAATATA 
GGACATGAAG TTGGCTATAT TGTGCCGTTC GAAAACTGCT GTACAAGCGA GACAATTTTA 
AGGTATTGCA CGGACGAGAT GTTGCTGAGG GAAATGATGT CCAGCCCCCT TCTGTCCAGT 
TATGGAGTCG TCATTATCGA TGATGTCTAT GAAAGATCAG TTTCTACTGA TGTGCTGCTC 
AGTTGCCTTA AGGTCATTGC AGTCTCAAGA CCTGAACTGA AAGTTGTGAT AATTACCTGC 
CCTGCTCTGT CTGGGACCTT GGTTTCTTAT TATGGAAATG CTCCACTTGT AGAAACAGAA 
AACATGCACT CTGTAGAGTC GGTTTACACC ACAAGCATTC CAAGAGACTA CTTCCACTCA 
ACTTTACGAC TTCTCTTTGA AATACATCAT ACTAAAGAAC AAGGGGACAT TGTTGTCTTT 
CTGGCCTGTG AAGAAGAGAT AAAAAGGGCA TATGAGCATA TCAAACAGGA AGCATTGCAC 
ATGAATCCAG AATTGGGTGA ACTGGTACCC ATTGCTTTGT ACCCCAATCA AAGCATTTCA 
GACTACATAC CTTATGAGGA ACTGGATGAC AACAGCAAAA GCCCAAAAAG AAAAGTGGTA 
TTGACGACCA GCCTTGGCGA GTCTCTCATC TGGATGAAAA ATATCCTCTT TGTCATTGAT 
GTTGGTATAG AGAGGAGGAA GGTATATAAT ACCAGAATCC GCGCAGAATC ACATGTCACG 
CAACCAATAA GTAAGGTCCG GGCTGAGATG AGGAAGCATA TACTGAGCTC ATATTCTAAA 
GGAAAACTAT TTTGCTTGTA CCCCGAGGAC TTTGCACATG AGATGAAACC ATTCCTGACT 
GCAAAAGTGG AAGAGTGCAA TTTGATTTCA ATGGTTCTGT TTCTGAAGAG AATGGACATT 
GCGGGGCTCG CTCACTGTGA CTTCATCAAC AGACCAGACC CTGAAAGTTT AATGCAAGCA 
CTGGAAGATC TGGATTATTT GGCAGCACTT GACAATGATG GAAATCTTTC TGAATTTGGA 
ATAATTATGT CTGAGTTTCC ACTCGATCCA CAGCTGTCAA AATCCATTTT AGCTGCCTGC 
GAATTCGACT GCGTGGATGA AATGCTCACA CTGGCTGCTA TGGTTACAGC CCCGAATTGT 
TTTATCGATT CATCCCCTGA AGCCGAAGAG CTGATTTTAA TTCGTAAAGG AAAGTTTTCT 
CATCCAGAGG GGGACCACTT CACTCTTATA AATATCTACA ATGAATATGA GCAGATGAAG 
AGAAGCAATG CTAGCCAGTA CGATGTTGAA AAATGGTGTC AGGATCACTG CCTAAGTTTC 
CTGGCAATGG AGATGGCGCG TGCCATAAGA AATGAGCTCG CTGACATTAT GAGGCGCATA 
GAGCTTCCAC TGACAGCGCC TGCATTTGGC TCTGAGGAGA ATGTAATAAA CATTAAAAAG 
TCACTTTTGT CAGGATACTT CATGCAAATT GCTCGTGATG TTGATGGTTT GGGAAACTAC 
ATTATGCTGA CACACAAGCA GGTGGGACAA CTCCATCCTG ATTCTGGCTT CAACGGCGCC 
AAGGTTCCAG AATGGGTTCT CTACCACGAA TTTAGCGTCT CTGAGAGAAG CTGCATCAGG 
ATAGTCTCAG AGATCTCACC AAACATGTTC ATGGAGTTCG TTCCCCCGTA CTACTTCAGC 
AACCTGCCCC CTAGTGAGAC TAAAGAGCTC CTGCAGCAGC ACCAGACTGT GCCCAGTTCC 
CCGGCTGAAG AGCCCGGAGA AGAGGAGCCC CTACCTGATG CCGATGATGA AGGTGCAACA 
GAACAAAGAT GCATTATACA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx32 ( XM_018095827.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951316.1 Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa07399
Clone ID Related Accession (Same CDS sequence)	XM_004912720.3 , XM_018095827.1 , XM_012959634.2 , XM_002932650.4
Accession Version	XM_018095827.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTT CACTAGAGGC CTATGAACAG GACGGCGATG AACTCATTTC CGAAGAGAAA 
AGCTGTTGCC TCGATGTTAC AGACTCAAGT GATGTGGAAG GAGATGAACT TAATGACTTG 
GAACTAAATC CATTTGATGG TTTGCCTTAC TCATCTCGTT TTTACAAACT CTTAAAAGAA 
AGAGAAGATC TTCCCATATG GAAAGCCAAA TATGACTTTT TGGAGCGATT ATCTAACAGC 
CAAGTTGTGG TTGTGTCGGC GGGACCTAAG AGCGGAAAGA GTTCACAAAT TCCTCAATGG 
TGCGCAGAAT ACTGCTTAGC AGGGCACTAT CAACATGGGG CAGTTGTGTG TACGCAGGCG 
CACAAGCAGA CGGCAGTATG GCTGGCCATG AGAGTGGCAG ACGAAATGGA TGTCAATATA 
GGACATGAAG TTGGCTATAT TGTGCCGTTC GAAAACTGCT GTACAAGCGA GACAATTTTA 
AGGTATTGCA CGGACGAGAT GTTGCTGAGG GAAATGATGT CCAGCCCCCT TCTGTCCAGT 
TATGGAGTCG TCATTATCGA TGATGTCTAT GAAAGATCAG TTTCTACTGA TGTGCTGCTC 
AGTTGCCTTA AGGTCATTGC AGTCTCAAGA CCTGAACTGA AAGTTGTGAT AATTACCTGC 
CCTGCTCTGT CTGGGACCTT GGTTTCTTAT TATGGAAATG CTCCACTTGT AGAAACAGAA 
AACATGCACT CTGTAGAGTC GGTTTACACC ACAAGCATTC CAAGAGACTA CTTCCACTCA 
ACTTTACGAC TTCTCTTTGA AATACATCAT ACTAAAGAAC AAGGGGACAT TGTTGTCTTT 
CTGGCCTGTG AAGAAGAGAT AAAAAGGGCA TATGAGCATA TCAAACAGGA AGCATTGCAC 
ATGAATCCAG AATTGGGTGA ACTGGTACCC ATTGCTTTGT ACCCCAATCA AAGCATTTCA 
GACTACATAC CTTATGAGGA ACTGGATGAC AACAGCAAAA GCCCAAAAAG AAAAGTGGTA 
TTGACGACCA GCCTTGGCGA GTCTCTCATC TGGATGAAAA ATATCCTCTT TGTCATTGAT 
GTTGGTATAG AGAGGAGGAA GGTATATAAT ACCAGAATCC GCGCAGAATC ACATGTCACG 
CAACCAATAA GTAAGGTCCG GGCTGAGATG AGGAAGCATA TACTGAGCTC ATATTCTAAA 
GGAAAACTAT TTTGCTTGTA CCCCGAGGAC TTTGCACATG AGATGAAACC ATTCCTGACT 
GCAAAAGTGG AAGAGTGCAA TTTGATTTCA ATGGTTCTGT TTCTGAAGAG AATGGACATT 
GCGGGGCTCG CTCACTGTGA CTTCATCAAC AGACCAGACC CTGAAAGTTT AATGCAAGCA 
CTGGAAGATC TGGATTATTT GGCAGCACTT GACAATGATG GAAATCTTTC TGAATTTGGA 
ATAATTATGT CTGAGTTTCC ACTCGATCCA CAGCTGTCAA AATCCATTTT AGCTGCCTGC 
GAATTCGACT GCGTGGATGA AATGCTCACA CTGGCTGCTA TGGTTACAGC CCCGAATTGT 
TTTATCGATT CATCCCCTGA AGCCGAAGAG CTGATTTTAA TTCGTAAAGG AAAGTTTTCT 
CATCCAGAGG GGGACCACTT CACTCTTATA AATATCTACA ATGAATATGA GCAGATGAAG 
AGAAGCAATG CTAGCCAGTA CGATGTTGAA AAATGGTGTC AGGATCACTG CCTAAGTTTC 
CTGGCAATGG AGATGGCGCG TGCCATAAGA AATGAGCTCG CTGACATTAT GAGGCGCATA 
GAGCTTCCAC TGACAGCGCC TGCATTTGGC TCTGAGGAGA ATGTAATAAA CATTAAAAAG 
TCACTTTTGT CAGGATACTT CATGCAAATT GCTCGTGATG TTGATGGTTT GGGAAACTAC 
ATTATGCTGA CACACAAGCA GGTGGGACAA CTCCATCCTG ATTCTGGCTT CAACGGCGCC 
AAGGTTCCAG AATGGGTTCT CTACCACGAA TTTAGCGTCT CTGAGAGAAG CTGCATCAGG 
ATAGTCTCAG AGATCTCACC AAACATGTTC ATGGAGTTCG TTCCCCCGTA CTACTTCAGC 
AACCTGCCCC CTAGTGAGAC TAAAGAGCTC CTGCAGCAGC ACCAGACTGT GCCCAGTTCC 
CCGGCTGAAG AGCCCGGAGA AGAGGAGCCC CTACCTGATG CCGATGATGA AGGTGCAACA 
GAACAAAGAT GCATTATACA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951316.1
CDS	245..2488
Translation	MALSLEAYEQDGDELISEEKSCCLDVTDSSDVEGDELNDLELNPFDGLPYSSRFYKLLKEREDLPIWKAKYDFLERLSNSQVVVVSAGPKSGKSSQIPQWCAEYCLAGHYQHGAVVCTQAHKQTAVWLAMRVADEMDVNIGHEVGYIVPFENCCTSETILRYCTDEMLLREMMSSPLLSSYGVVIIDDVYERSVSTDVLLSCLKVIAVSRPELKVVIITCPALSGTLVSYYGNAPLVETENMHSVESVYTTSIPRDYFHSTLRLLFEIHHTKEQGDIVVFLACEEEIKRAYEHIKQEALHMNPELGELVPIALYPNQSISDYIPYEELDDNSKSPKRKVVLTTSLGESLIWMKNILFVIDVGIERRKVYNTRIRAESHVTQPISKVRAEMRKHILSSYSKGKLFCLYPEDFAHEMKPFLTAKVEECNLISMVLFLKRMDIAGLAHCDFINRPDPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILAACEFDCVDEMLTLAAMVTAPNCFIDSSPEAEELILIRKGKFSHPEGDHFTLINIYNEYEQMKRSNASQYDVEKWCQDHCLSFLAMEMARAIRNELADIMRRIELPLTAPAFGSEENVINIKKSLLSGYFMQIARDVDGLGNYIMLTHKQVGQLHPDSGFNGAKVPEWVLYHEFSVSERSCIRIVSEISPNMFMEFVPPYYFSNLPPSETKELLQQHQTVPSSPAEEPGEEEPLPDADDEGATEQRCIIQ

Target ORF information:

RefSeq Version	XM_018095827.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095827.1

ORF Insert Sequence:

ATGGCTCTTT CACTAGAGGC CTATGAACAG GACGGCGATG AACTCATTTC CGAAGAGAAA 
AGCTGTTGCC TCGATGTTAC AGACTCAAGT GATGTGGAAG GAGATGAACT TAATGACTTG 
GAACTAAATC CATTTGATGG TTTGCCTTAC TCATCTCGTT TTTACAAACT CTTAAAAGAA 
AGAGAAGATC TTCCCATATG GAAAGCCAAA TATGACTTTT TGGAGCGATT ATCTAACAGC 
CAAGTTGTGG TTGTGTCGGC GGGACCTAAG AGCGGAAAGA GTTCACAAAT TCCTCAATGG 
TGCGCAGAAT ACTGCTTAGC AGGGCACTAT CAACATGGGG CAGTTGTGTG TACGCAGGCG 
CACAAGCAGA CGGCAGTATG GCTGGCCATG AGAGTGGCAG ACGAAATGGA TGTCAATATA 
GGACATGAAG TTGGCTATAT TGTGCCGTTC GAAAACTGCT GTACAAGCGA GACAATTTTA 
AGGTATTGCA CGGACGAGAT GTTGCTGAGG GAAATGATGT CCAGCCCCCT TCTGTCCAGT 
TATGGAGTCG TCATTATCGA TGATGTCTAT GAAAGATCAG TTTCTACTGA TGTGCTGCTC 
AGTTGCCTTA AGGTCATTGC AGTCTCAAGA CCTGAACTGA AAGTTGTGAT AATTACCTGC 
CCTGCTCTGT CTGGGACCTT GGTTTCTTAT TATGGAAATG CTCCACTTGT AGAAACAGAA 
AACATGCACT CTGTAGAGTC GGTTTACACC ACAAGCATTC CAAGAGACTA CTTCCACTCA 
ACTTTACGAC TTCTCTTTGA AATACATCAT ACTAAAGAAC AAGGGGACAT TGTTGTCTTT 
CTGGCCTGTG AAGAAGAGAT AAAAAGGGCA TATGAGCATA TCAAACAGGA AGCATTGCAC 
ATGAATCCAG AATTGGGTGA ACTGGTACCC ATTGCTTTGT ACCCCAATCA AAGCATTTCA 
GACTACATAC CTTATGAGGA ACTGGATGAC AACAGCAAAA GCCCAAAAAG AAAAGTGGTA 
TTGACGACCA GCCTTGGCGA GTCTCTCATC TGGATGAAAA ATATCCTCTT TGTCATTGAT 
GTTGGTATAG AGAGGAGGAA GGTATATAAT ACCAGAATCC GCGCAGAATC ACATGTCACG 
CAACCAATAA GTAAGGTCCG GGCTGAGATG AGGAAGCATA TACTGAGCTC ATATTCTAAA 
GGAAAACTAT TTTGCTTGTA CCCCGAGGAC TTTGCACATG AGATGAAACC ATTCCTGACT 
GCAAAAGTGG AAGAGTGCAA TTTGATTTCA ATGGTTCTGT TTCTGAAGAG AATGGACATT 
GCGGGGCTCG CTCACTGTGA CTTCATCAAC AGACCAGACC CTGAAAGTTT AATGCAAGCA 
CTGGAAGATC TGGATTATTT GGCAGCACTT GACAATGATG GAAATCTTTC TGAATTTGGA 
ATAATTATGT CTGAGTTTCC ACTCGATCCA CAGCTGTCAA AATCCATTTT AGCTGCCTGC 
GAATTCGACT GCGTGGATGA AATGCTCACA CTGGCTGCTA TGGTTACAGC CCCGAATTGT 
TTTATCGATT CATCCCCTGA AGCCGAAGAG CTGATTTTAA TTCGTAAAGG AAAGTTTTCT 
CATCCAGAGG GGGACCACTT CACTCTTATA AATATCTACA ATGAATATGA GCAGATGAAG 
AGAAGCAATG CTAGCCAGTA CGATGTTGAA AAATGGTGTC AGGATCACTG CCTAAGTTTC 
CTGGCAATGG AGATGGCGCG TGCCATAAGA AATGAGCTCG CTGACATTAT GAGGCGCATA 
GAGCTTCCAC TGACAGCGCC TGCATTTGGC TCTGAGGAGA ATGTAATAAA CATTAAAAAG 
TCACTTTTGT CAGGATACTT CATGCAAATT GCTCGTGATG TTGATGGTTT GGGAAACTAC 
ATTATGCTGA CACACAAGCA GGTGGGACAA CTCCATCCTG ATTCTGGCTT CAACGGCGCC 
AAGGTTCCAG AATGGGTTCT CTACCACGAA TTTAGCGTCT CTGAGAGAAG CTGCATCAGG 
ATAGTCTCAG AGATCTCACC AAACATGTTC ATGGAGTTCG TTCCCCCGTA CTACTTCAGC 
AACCTGCCCC CTAGTGAGAC TAAAGAGCTC CTGCAGCAGC ACCAGACTGT GCCCAGTTCC 
CCGGCTGAAG AGCCCGGAGA AGAGGAGCCC CTACCTGATG CCGATGATGA AGGTGCAACA 
GAACAAAGAT GCATTATACA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx32 ( XM_012959634.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012815088.1 Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa07399
Clone ID Related Accession (Same CDS sequence)	XM_004912720.3 , XM_018095827.1 , XM_012959634.2 , XM_002932650.4
Accession Version	XM_012959634.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959634.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTT CACTAGAGGC CTATGAACAG GACGGCGATG AACTCATTTC CGAAGAGAAA 
AGCTGTTGCC TCGATGTTAC AGACTCAAGT GATGTGGAAG GAGATGAACT TAATGACTTG 
GAACTAAATC CATTTGATGG TTTGCCTTAC TCATCTCGTT TTTACAAACT CTTAAAAGAA 
AGAGAAGATC TTCCCATATG GAAAGCCAAA TATGACTTTT TGGAGCGATT ATCTAACAGC 
CAAGTTGTGG TTGTGTCGGC GGGACCTAAG AGCGGAAAGA GTTCACAAAT TCCTCAATGG 
TGCGCAGAAT ACTGCTTAGC AGGGCACTAT CAACATGGGG CAGTTGTGTG TACGCAGGCG 
CACAAGCAGA CGGCAGTATG GCTGGCCATG AGAGTGGCAG ACGAAATGGA TGTCAATATA 
GGACATGAAG TTGGCTATAT TGTGCCGTTC GAAAACTGCT GTACAAGCGA GACAATTTTA 
AGGTATTGCA CGGACGAGAT GTTGCTGAGG GAAATGATGT CCAGCCCCCT TCTGTCCAGT 
TATGGAGTCG TCATTATCGA TGATGTCTAT GAAAGATCAG TTTCTACTGA TGTGCTGCTC 
AGTTGCCTTA AGGTCATTGC AGTCTCAAGA CCTGAACTGA AAGTTGTGAT AATTACCTGC 
CCTGCTCTGT CTGGGACCTT GGTTTCTTAT TATGGAAATG CTCCACTTGT AGAAACAGAA 
AACATGCACT CTGTAGAGTC GGTTTACACC ACAAGCATTC CAAGAGACTA CTTCCACTCA 
ACTTTACGAC TTCTCTTTGA AATACATCAT ACTAAAGAAC AAGGGGACAT TGTTGTCTTT 
CTGGCCTGTG AAGAAGAGAT AAAAAGGGCA TATGAGCATA TCAAACAGGA AGCATTGCAC 
ATGAATCCAG AATTGGGTGA ACTGGTACCC ATTGCTTTGT ACCCCAATCA AAGCATTTCA 
GACTACATAC CTTATGAGGA ACTGGATGAC AACAGCAAAA GCCCAAAAAG AAAAGTGGTA 
TTGACGACCA GCCTTGGCGA GTCTCTCATC TGGATGAAAA ATATCCTCTT TGTCATTGAT 
GTTGGTATAG AGAGGAGGAA GGTATATAAT ACCAGAATCC GCGCAGAATC ACATGTCACG 
CAACCAATAA GTAAGGTCCG GGCTGAGATG AGGAAGCATA TACTGAGCTC ATATTCTAAA 
GGAAAACTAT TTTGCTTGTA CCCCGAGGAC TTTGCACATG AGATGAAACC ATTCCTGACT 
GCAAAAGTGG AAGAGTGCAA TTTGATTTCA ATGGTTCTGT TTCTGAAGAG AATGGACATT 
GCGGGGCTCG CTCACTGTGA CTTCATCAAC AGACCAGACC CTGAAAGTTT AATGCAAGCA 
CTGGAAGATC TGGATTATTT GGCAGCACTT GACAATGATG GAAATCTTTC TGAATTTGGA 
ATAATTATGT CTGAGTTTCC ACTCGATCCA CAGCTGTCAA AATCCATTTT AGCTGCCTGC 
GAATTCGACT GCGTGGATGA AATGCTCACA CTGGCTGCTA TGGTTACAGC CCCGAATTGT 
TTTATCGATT CATCCCCTGA AGCCGAAGAG CTGATTTTAA TTCGTAAAGG AAAGTTTTCT 
CATCCAGAGG GGGACCACTT CACTCTTATA AATATCTACA ATGAATATGA GCAGATGAAG 
AGAAGCAATG CTAGCCAGTA CGATGTTGAA AAATGGTGTC AGGATCACTG CCTAAGTTTC 
CTGGCAATGG AGATGGCGCG TGCCATAAGA AATGAGCTCG CTGACATTAT GAGGCGCATA 
GAGCTTCCAC TGACAGCGCC TGCATTTGGC TCTGAGGAGA ATGTAATAAA CATTAAAAAG 
TCACTTTTGT CAGGATACTT CATGCAAATT GCTCGTGATG TTGATGGTTT GGGAAACTAC 
ATTATGCTGA CACACAAGCA GGTGGGACAA CTCCATCCTG ATTCTGGCTT CAACGGCGCC 
AAGGTTCCAG AATGGGTTCT CTACCACGAA TTTAGCGTCT CTGAGAGAAG CTGCATCAGG 
ATAGTCTCAG AGATCTCACC AAACATGTTC ATGGAGTTCG TTCCCCCGTA CTACTTCAGC 
AACCTGCCCC CTAGTGAGAC TAAAGAGCTC CTGCAGCAGC ACCAGACTGT GCCCAGTTCC 
CCGGCTGAAG AGCCCGGAGA AGAGGAGCCC CTACCTGATG CCGATGATGA AGGTGCAACA 
GAACAAAGAT GCATTATACA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012815088.1
CDS	349..2592
Translation	MALSLEAYEQDGDELISEEKSCCLDVTDSSDVEGDELNDLELNPFDGLPYSSRFYKLLKEREDLPIWKAKYDFLERLSNSQVVVVSAGPKSGKSSQIPQWCAEYCLAGHYQHGAVVCTQAHKQTAVWLAMRVADEMDVNIGHEVGYIVPFENCCTSETILRYCTDEMLLREMMSSPLLSSYGVVIIDDVYERSVSTDVLLSCLKVIAVSRPELKVVIITCPALSGTLVSYYGNAPLVETENMHSVESVYTTSIPRDYFHSTLRLLFEIHHTKEQGDIVVFLACEEEIKRAYEHIKQEALHMNPELGELVPIALYPNQSISDYIPYEELDDNSKSPKRKVVLTTSLGESLIWMKNILFVIDVGIERRKVYNTRIRAESHVTQPISKVRAEMRKHILSSYSKGKLFCLYPEDFAHEMKPFLTAKVEECNLISMVLFLKRMDIAGLAHCDFINRPDPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILAACEFDCVDEMLTLAAMVTAPNCFIDSSPEAEELILIRKGKFSHPEGDHFTLINIYNEYEQMKRSNASQYDVEKWCQDHCLSFLAMEMARAIRNELADIMRRIELPLTAPAFGSEENVINIKKSLLSGYFMQIARDVDGLGNYIMLTHKQVGQLHPDSGFNGAKVPEWVLYHEFSVSERSCIRIVSEISPNMFMEFVPPYYFSNLPPSETKELLQQHQTVPSSPAEEPGEEEPLPDADDEGATEQRCIIQ

Target ORF information:

RefSeq Version	XM_012959634.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012959634.2

ORF Insert Sequence:

ATGGCTCTTT CACTAGAGGC CTATGAACAG GACGGCGATG AACTCATTTC CGAAGAGAAA 
AGCTGTTGCC TCGATGTTAC AGACTCAAGT GATGTGGAAG GAGATGAACT TAATGACTTG 
GAACTAAATC CATTTGATGG TTTGCCTTAC TCATCTCGTT TTTACAAACT CTTAAAAGAA 
AGAGAAGATC TTCCCATATG GAAAGCCAAA TATGACTTTT TGGAGCGATT ATCTAACAGC 
CAAGTTGTGG TTGTGTCGGC GGGACCTAAG AGCGGAAAGA GTTCACAAAT TCCTCAATGG 
TGCGCAGAAT ACTGCTTAGC AGGGCACTAT CAACATGGGG CAGTTGTGTG TACGCAGGCG 
CACAAGCAGA CGGCAGTATG GCTGGCCATG AGAGTGGCAG ACGAAATGGA TGTCAATATA 
GGACATGAAG TTGGCTATAT TGTGCCGTTC GAAAACTGCT GTACAAGCGA GACAATTTTA 
AGGTATTGCA CGGACGAGAT GTTGCTGAGG GAAATGATGT CCAGCCCCCT TCTGTCCAGT 
TATGGAGTCG TCATTATCGA TGATGTCTAT GAAAGATCAG TTTCTACTGA TGTGCTGCTC 
AGTTGCCTTA AGGTCATTGC AGTCTCAAGA CCTGAACTGA AAGTTGTGAT AATTACCTGC 
CCTGCTCTGT CTGGGACCTT GGTTTCTTAT TATGGAAATG CTCCACTTGT AGAAACAGAA 
AACATGCACT CTGTAGAGTC GGTTTACACC ACAAGCATTC CAAGAGACTA CTTCCACTCA 
ACTTTACGAC TTCTCTTTGA AATACATCAT ACTAAAGAAC AAGGGGACAT TGTTGTCTTT 
CTGGCCTGTG AAGAAGAGAT AAAAAGGGCA TATGAGCATA TCAAACAGGA AGCATTGCAC 
ATGAATCCAG AATTGGGTGA ACTGGTACCC ATTGCTTTGT ACCCCAATCA AAGCATTTCA 
GACTACATAC CTTATGAGGA ACTGGATGAC AACAGCAAAA GCCCAAAAAG AAAAGTGGTA 
TTGACGACCA GCCTTGGCGA GTCTCTCATC TGGATGAAAA ATATCCTCTT TGTCATTGAT 
GTTGGTATAG AGAGGAGGAA GGTATATAAT ACCAGAATCC GCGCAGAATC ACATGTCACG 
CAACCAATAA GTAAGGTCCG GGCTGAGATG AGGAAGCATA TACTGAGCTC ATATTCTAAA 
GGAAAACTAT TTTGCTTGTA CCCCGAGGAC TTTGCACATG AGATGAAACC ATTCCTGACT 
GCAAAAGTGG AAGAGTGCAA TTTGATTTCA ATGGTTCTGT TTCTGAAGAG AATGGACATT 
GCGGGGCTCG CTCACTGTGA CTTCATCAAC AGACCAGACC CTGAAAGTTT AATGCAAGCA 
CTGGAAGATC TGGATTATTT GGCAGCACTT GACAATGATG GAAATCTTTC TGAATTTGGA 
ATAATTATGT CTGAGTTTCC ACTCGATCCA CAGCTGTCAA AATCCATTTT AGCTGCCTGC 
GAATTCGACT GCGTGGATGA AATGCTCACA CTGGCTGCTA TGGTTACAGC CCCGAATTGT 
TTTATCGATT CATCCCCTGA AGCCGAAGAG CTGATTTTAA TTCGTAAAGG AAAGTTTTCT 
CATCCAGAGG GGGACCACTT CACTCTTATA AATATCTACA ATGAATATGA GCAGATGAAG 
AGAAGCAATG CTAGCCAGTA CGATGTTGAA AAATGGTGTC AGGATCACTG CCTAAGTTTC 
CTGGCAATGG AGATGGCGCG TGCCATAAGA AATGAGCTCG CTGACATTAT GAGGCGCATA 
GAGCTTCCAC TGACAGCGCC TGCATTTGGC TCTGAGGAGA ATGTAATAAA CATTAAAAAG 
TCACTTTTGT CAGGATACTT CATGCAAATT GCTCGTGATG TTGATGGTTT GGGAAACTAC 
ATTATGCTGA CACACAAGCA GGTGGGACAA CTCCATCCTG ATTCTGGCTT CAACGGCGCC 
AAGGTTCCAG AATGGGTTCT CTACCACGAA TTTAGCGTCT CTGAGAGAAG CTGCATCAGG 
ATAGTCTCAG AGATCTCACC AAACATGTTC ATGGAGTTCG TTCCCCCGTA CTACTTCAGC 
AACCTGCCCC CTAGTGAGAC TAAAGAGCTC CTGCAGCAGC ACCAGACTGT GCCCAGTTCC 
CCGGCTGAAG AGCCCGGAGA AGAGGAGCCC CTACCTGATG CCGATGATGA AGGTGCAACA 
GAACAAAGAT GCATTATACA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx32 ( XM_002932650.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932696.2 Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa07399
Clone ID Related Accession (Same CDS sequence)	XM_004912720.3 , XM_018095827.1 , XM_012959634.2 , XM_002932650.4
Accession Version	XM_002932650.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932650.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTT CACTAGAGGC CTATGAACAG GACGGCGATG AACTCATTTC CGAAGAGAAA 
AGCTGTTGCC TCGATGTTAC AGACTCAAGT GATGTGGAAG GAGATGAACT TAATGACTTG 
GAACTAAATC CATTTGATGG TTTGCCTTAC TCATCTCGTT TTTACAAACT CTTAAAAGAA 
AGAGAAGATC TTCCCATATG GAAAGCCAAA TATGACTTTT TGGAGCGATT ATCTAACAGC 
CAAGTTGTGG TTGTGTCGGC GGGACCTAAG AGCGGAAAGA GTTCACAAAT TCCTCAATGG 
TGCGCAGAAT ACTGCTTAGC AGGGCACTAT CAACATGGGG CAGTTGTGTG TACGCAGGCG 
CACAAGCAGA CGGCAGTATG GCTGGCCATG AGAGTGGCAG ACGAAATGGA TGTCAATATA 
GGACATGAAG TTGGCTATAT TGTGCCGTTC GAAAACTGCT GTACAAGCGA GACAATTTTA 
AGGTATTGCA CGGACGAGAT GTTGCTGAGG GAAATGATGT CCAGCCCCCT TCTGTCCAGT 
TATGGAGTCG TCATTATCGA TGATGTCTAT GAAAGATCAG TTTCTACTGA TGTGCTGCTC 
AGTTGCCTTA AGGTCATTGC AGTCTCAAGA CCTGAACTGA AAGTTGTGAT AATTACCTGC 
CCTGCTCTGT CTGGGACCTT GGTTTCTTAT TATGGAAATG CTCCACTTGT AGAAACAGAA 
AACATGCACT CTGTAGAGTC GGTTTACACC ACAAGCATTC CAAGAGACTA CTTCCACTCA 
ACTTTACGAC TTCTCTTTGA AATACATCAT ACTAAAGAAC AAGGGGACAT TGTTGTCTTT 
CTGGCCTGTG AAGAAGAGAT AAAAAGGGCA TATGAGCATA TCAAACAGGA AGCATTGCAC 
ATGAATCCAG AATTGGGTGA ACTGGTACCC ATTGCTTTGT ACCCCAATCA AAGCATTTCA 
GACTACATAC CTTATGAGGA ACTGGATGAC AACAGCAAAA GCCCAAAAAG AAAAGTGGTA 
TTGACGACCA GCCTTGGCGA GTCTCTCATC TGGATGAAAA ATATCCTCTT TGTCATTGAT 
GTTGGTATAG AGAGGAGGAA GGTATATAAT ACCAGAATCC GCGCAGAATC ACATGTCACG 
CAACCAATAA GTAAGGTCCG GGCTGAGATG AGGAAGCATA TACTGAGCTC ATATTCTAAA 
GGAAAACTAT TTTGCTTGTA CCCCGAGGAC TTTGCACATG AGATGAAACC ATTCCTGACT 
GCAAAAGTGG AAGAGTGCAA TTTGATTTCA ATGGTTCTGT TTCTGAAGAG AATGGACATT 
GCGGGGCTCG CTCACTGTGA CTTCATCAAC AGACCAGACC CTGAAAGTTT AATGCAAGCA 
CTGGAAGATC TGGATTATTT GGCAGCACTT GACAATGATG GAAATCTTTC TGAATTTGGA 
ATAATTATGT CTGAGTTTCC ACTCGATCCA CAGCTGTCAA AATCCATTTT AGCTGCCTGC 
GAATTCGACT GCGTGGATGA AATGCTCACA CTGGCTGCTA TGGTTACAGC CCCGAATTGT 
TTTATCGATT CATCCCCTGA AGCCGAAGAG CTGATTTTAA TTCGTAAAGG AAAGTTTTCT 
CATCCAGAGG GGGACCACTT CACTCTTATA AATATCTACA ATGAATATGA GCAGATGAAG 
AGAAGCAATG CTAGCCAGTA CGATGTTGAA AAATGGTGTC AGGATCACTG CCTAAGTTTC 
CTGGCAATGG AGATGGCGCG TGCCATAAGA AATGAGCTCG CTGACATTAT GAGGCGCATA 
GAGCTTCCAC TGACAGCGCC TGCATTTGGC TCTGAGGAGA ATGTAATAAA CATTAAAAAG 
TCACTTTTGT CAGGATACTT CATGCAAATT GCTCGTGATG TTGATGGTTT GGGAAACTAC 
ATTATGCTGA CACACAAGCA GGTGGGACAA CTCCATCCTG ATTCTGGCTT CAACGGCGCC 
AAGGTTCCAG AATGGGTTCT CTACCACGAA TTTAGCGTCT CTGAGAGAAG CTGCATCAGG 
ATAGTCTCAG AGATCTCACC AAACATGTTC ATGGAGTTCG TTCCCCCGTA CTACTTCAGC 
AACCTGCCCC CTAGTGAGAC TAAAGAGCTC CTGCAGCAGC ACCAGACTGT GCCCAGTTCC 
CCGGCTGAAG AGCCCGGAGA AGAGGAGCCC CTACCTGATG CCGATGATGA AGGTGCAACA 
GAACAAAGAT GCATTATACA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932696.2
CDS	284..2527
Translation	MALSLEAYEQDGDELISEEKSCCLDVTDSSDVEGDELNDLELNPFDGLPYSSRFYKLLKEREDLPIWKAKYDFLERLSNSQVVVVSAGPKSGKSSQIPQWCAEYCLAGHYQHGAVVCTQAHKQTAVWLAMRVADEMDVNIGHEVGYIVPFENCCTSETILRYCTDEMLLREMMSSPLLSSYGVVIIDDVYERSVSTDVLLSCLKVIAVSRPELKVVIITCPALSGTLVSYYGNAPLVETENMHSVESVYTTSIPRDYFHSTLRLLFEIHHTKEQGDIVVFLACEEEIKRAYEHIKQEALHMNPELGELVPIALYPNQSISDYIPYEELDDNSKSPKRKVVLTTSLGESLIWMKNILFVIDVGIERRKVYNTRIRAESHVTQPISKVRAEMRKHILSSYSKGKLFCLYPEDFAHEMKPFLTAKVEECNLISMVLFLKRMDIAGLAHCDFINRPDPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILAACEFDCVDEMLTLAAMVTAPNCFIDSSPEAEELILIRKGKFSHPEGDHFTLINIYNEYEQMKRSNASQYDVEKWCQDHCLSFLAMEMARAIRNELADIMRRIELPLTAPAFGSEENVINIKKSLLSGYFMQIARDVDGLGNYIMLTHKQVGQLHPDSGFNGAKVPEWVLYHEFSVSERSCIRIVSEISPNMFMEFVPPYYFSNLPPSETKELLQQHQTVPSSPAEEPGEEEPLPDADDEGATEQRCIIQ

Target ORF information:

RefSeq Version	XM_002932650.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932650.4

ORF Insert Sequence:

ATGGCTCTTT CACTAGAGGC CTATGAACAG GACGGCGATG AACTCATTTC CGAAGAGAAA 
AGCTGTTGCC TCGATGTTAC AGACTCAAGT GATGTGGAAG GAGATGAACT TAATGACTTG 
GAACTAAATC CATTTGATGG TTTGCCTTAC TCATCTCGTT TTTACAAACT CTTAAAAGAA 
AGAGAAGATC TTCCCATATG GAAAGCCAAA TATGACTTTT TGGAGCGATT ATCTAACAGC 
CAAGTTGTGG TTGTGTCGGC GGGACCTAAG AGCGGAAAGA GTTCACAAAT TCCTCAATGG 
TGCGCAGAAT ACTGCTTAGC AGGGCACTAT CAACATGGGG CAGTTGTGTG TACGCAGGCG 
CACAAGCAGA CGGCAGTATG GCTGGCCATG AGAGTGGCAG ACGAAATGGA TGTCAATATA 
GGACATGAAG TTGGCTATAT TGTGCCGTTC GAAAACTGCT GTACAAGCGA GACAATTTTA 
AGGTATTGCA CGGACGAGAT GTTGCTGAGG GAAATGATGT CCAGCCCCCT TCTGTCCAGT 
TATGGAGTCG TCATTATCGA TGATGTCTAT GAAAGATCAG TTTCTACTGA TGTGCTGCTC 
AGTTGCCTTA AGGTCATTGC AGTCTCAAGA CCTGAACTGA AAGTTGTGAT AATTACCTGC 
CCTGCTCTGT CTGGGACCTT GGTTTCTTAT TATGGAAATG CTCCACTTGT AGAAACAGAA 
AACATGCACT CTGTAGAGTC GGTTTACACC ACAAGCATTC CAAGAGACTA CTTCCACTCA 
ACTTTACGAC TTCTCTTTGA AATACATCAT ACTAAAGAAC AAGGGGACAT TGTTGTCTTT 
CTGGCCTGTG AAGAAGAGAT AAAAAGGGCA TATGAGCATA TCAAACAGGA AGCATTGCAC 
ATGAATCCAG AATTGGGTGA ACTGGTACCC ATTGCTTTGT ACCCCAATCA AAGCATTTCA 
GACTACATAC CTTATGAGGA ACTGGATGAC AACAGCAAAA GCCCAAAAAG AAAAGTGGTA 
TTGACGACCA GCCTTGGCGA GTCTCTCATC TGGATGAAAA ATATCCTCTT TGTCATTGAT 
GTTGGTATAG AGAGGAGGAA GGTATATAAT ACCAGAATCC GCGCAGAATC ACATGTCACG 
CAACCAATAA GTAAGGTCCG GGCTGAGATG AGGAAGCATA TACTGAGCTC ATATTCTAAA 
GGAAAACTAT TTTGCTTGTA CCCCGAGGAC TTTGCACATG AGATGAAACC ATTCCTGACT 
GCAAAAGTGG AAGAGTGCAA TTTGATTTCA ATGGTTCTGT TTCTGAAGAG AATGGACATT 
GCGGGGCTCG CTCACTGTGA CTTCATCAAC AGACCAGACC CTGAAAGTTT AATGCAAGCA 
CTGGAAGATC TGGATTATTT GGCAGCACTT GACAATGATG GAAATCTTTC TGAATTTGGA 
ATAATTATGT CTGAGTTTCC ACTCGATCCA CAGCTGTCAA AATCCATTTT AGCTGCCTGC 
GAATTCGACT GCGTGGATGA AATGCTCACA CTGGCTGCTA TGGTTACAGC CCCGAATTGT 
TTTATCGATT CATCCCCTGA AGCCGAAGAG CTGATTTTAA TTCGTAAAGG AAAGTTTTCT 
CATCCAGAGG GGGACCACTT CACTCTTATA AATATCTACA ATGAATATGA GCAGATGAAG 
AGAAGCAATG CTAGCCAGTA CGATGTTGAA AAATGGTGTC AGGATCACTG CCTAAGTTTC 
CTGGCAATGG AGATGGCGCG TGCCATAAGA AATGAGCTCG CTGACATTAT GAGGCGCATA 
GAGCTTCCAC TGACAGCGCC TGCATTTGGC TCTGAGGAGA ATGTAATAAA CATTAAAAAG 
TCACTTTTGT CAGGATACTT CATGCAAATT GCTCGTGATG TTGATGGTTT GGGAAACTAC 
ATTATGCTGA CACACAAGCA GGTGGGACAA CTCCATCCTG ATTCTGGCTT CAACGGCGCC 
AAGGTTCCAG AATGGGTTCT CTACCACGAA TTTAGCGTCT CTGAGAGAAG CTGCATCAGG 
ATAGTCTCAG AGATCTCACC AAACATGTTC ATGGAGTTCG TTCCCCCGTA CTACTTCAGC 
AACCTGCCCC CTAGTGAGAC TAAAGAGCTC CTGCAGCAGC ACCAGACTGT GCCCAGTTCC 
CCGGCTGAAG AGCCCGGAGA AGAGGAGCCC CTACCTGATG CCGATGATGA AGGTGCAACA 
GAACAAAGAT GCATTATACA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx32 ( XM_004912720.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912777.2 Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa62622
Clone ID Related Accession (Same CDS sequence)	XM_004912720.4 , XM_031906149.1 , XM_002932650.5
Accession Version	XM_004912720.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912720.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGG CTATATTGTG CCGTTCGAAA ACTGCTGTAC AAGCGAGACA ATTTTTAAGG 
TATTGCACGG ACGAGATGTT GCTGAGGGAA ATGATGTCCA GCCCCCTTCT GTCCAGTTAT 
GGAGTCGTCA TTATCGATGA TGTCTATGAA AGATCAGTTT CTACTGATGT GCTGCTCAGT 
TGCCTTAAGG TCATTGCAGT CTCAAGACCT GAACTGAAAG TTGTGATAAT TACCTGCCCT 
GCTCTGTCTG GGACCTTGGT TTCTTATTAT GGAAATGCTC CACTTGTAGA AACAGAAAAC 
ATGCACTCTG TAGAGTCGGT TTACACCACA AGCATTCCAA GAGACTACTT CCACTCAACT 
TTACGACTTC TCTTTGAAAT ACATCATACT AAAGAACAAG GGGACATTGT TGTCTTTCTG 
GCCTGTGAAG AAGAGATAAA AAGGGCATAT GAGCATATCA AACAGGAAGC ATTGCACATG 
AATCCAGAAT TGGGTGAACT GGTACCCATT GCTTTGTACC CCAATCAAAG CATTTCAGAC 
TACATACCTT ATGAGGAACT GGATGACAAC AGCAAAAGCC CAAAAAGAAA AGTGGTATTG 
ACGACCAGCC TTGGCGAGTC TCTCATCTGG ATGAAAAATA TCCTCTTTGT CATTGATGTT 
GGTATAGAGA GGAGGAAGGT ATATAATACC AGAATCCGCG CAGAATCACA TGTCACGCAA 
CCAATAAGTA AGGTCCGGGC TGAGATGAGG AAGCATATAC TGAGCTCATA TTCTAAAGGA 
AAACTATTTT GCTTGTACCC CGAGGACTTT GCACATGAGA TGAAACCATT CCTGACTGCA 
AAAGTGGAAG AGTGCAATTT GATTTCAATG GTTCTGTTTC TGAAGAGAAT GGACATTGCG 
GGGCTCGCTC ACTGTGACTT CATCAACAGA CCAGACCCTG AAAGTTTAAT GCAAGCACTG 
GAAGATCTGG ATTATTTGGC AGCACTTGAC AATGATGGAA ATCTTTCTGA ATTTGGAATA 
ATTATGTCTG AGTTTCCACT CGATCCACAG CTGTCAAAAT CCATTTTAGC TGCCTGCGAA 
TTCGACTGCG TGGATGAAAT GCTCACACTG GCTGCTATGG TTACAGCCCC GAATTGTTTT 
ATCGATTCAT CCCCTGAAGC CGAAGAGCTG ATTTTAATTC GTAAAGGAAA GTTTTCTCAT 
CCAGAGGGGG ACCACTTCAC TCTTATAAAT ATCTACAATG AATATGAGCA GATGAAGAGA 
AGCAATGCTA GCCAGTACGA TGTTGAAAAA TGGTGTCAGG ATCACTGCCT AAGTTTCCTG 
GCAATGGAGA TGGCGCGTGC CATAAGAAAT GAGCTCGCTG ACATTATGAG GCGCATAGAG 
CTTCCACTGA CAGCGCCTGC ATTTGGCTCT GAGGAGAATG TAATAAACAT TAAAAAGTCA 
CTTTTGTCAG GATACTTCAT GCAAATTGCT CGTGATGTTG ATGGTTTGGG AAACTACATT 
ATGCTGACAC ACAAGCAGGT GGGACAACTC CATCCTGATT CTGGCTTCAA CGGCGCCAAG 
GTTCCAGAAT GGGTTCTCTA CCACGAATTT AGCGTCTCTG AGAGAAGCTG CATCAGGATA 
GTCTCAGAGA TCTCACCAAA CATGTTCATG GAGTTCGTTC CCCCGTACTA CTTCAGCAAC 
CTGCCCCCTA GTGAGACTAA AGAGCTCCTG CAGCAGCACC AGACTGTGCC CAGTTCCCCG 
GCTGAAGAGC CCGGAGAAGA GGAGCCCCTA CCTGATGCCG ATGATGAAGG TGCAACAGAA 
CAAAGATGCA TTATACAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912777.2
CDS	785..2605
Translation	MKLAILCRSKTAVQARQFLRYCTDEMLLREMMSSPLLSSYGVVIIDDVYERSVSTDVLLSCLKVIAVSRPELKVVIITCPALSGTLVSYYGNAPLVETENMHSVESVYTTSIPRDYFHSTLRLLFEIHHTKEQGDIVVFLACEEEIKRAYEHIKQEALHMNPELGELVPIALYPNQSISDYIPYEELDDNSKSPKRKVVLTTSLGESLIWMKNILFVIDVGIERRKVYNTRIRAESHVTQPISKVRAEMRKHILSSYSKGKLFCLYPEDFAHEMKPFLTAKVEECNLISMVLFLKRMDIAGLAHCDFINRPDPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILAACEFDCVDEMLTLAAMVTAPNCFIDSSPEAEELILIRKGKFSHPEGDHFTLINIYNEYEQMKRSNASQYDVEKWCQDHCLSFLAMEMARAIRNELADIMRRIELPLTAPAFGSEENVINIKKSLLSGYFMQIARDVDGLGNYIMLTHKQVGQLHPDSGFNGAKVPEWVLYHEFSVSERSCIRIVSEISPNMFMEFVPPYYFSNLPPSETKELLQQHQTVPSSPAEEPGEEEPLPDADDEGATEQRCIIQ

Target ORF information:

RefSeq Version	XM_004912720.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004912720.4

ORF Insert Sequence:

ATGAAGTTGG CTATATTGTG CCGTTCGAAA ACTGCTGTAC AAGCGAGACA ATTTTTAAGG 
TATTGCACGG ACGAGATGTT GCTGAGGGAA ATGATGTCCA GCCCCCTTCT GTCCAGTTAT 
GGAGTCGTCA TTATCGATGA TGTCTATGAA AGATCAGTTT CTACTGATGT GCTGCTCAGT 
TGCCTTAAGG TCATTGCAGT CTCAAGACCT GAACTGAAAG TTGTGATAAT TACCTGCCCT 
GCTCTGTCTG GGACCTTGGT TTCTTATTAT GGAAATGCTC CACTTGTAGA AACAGAAAAC 
ATGCACTCTG TAGAGTCGGT TTACACCACA AGCATTCCAA GAGACTACTT CCACTCAACT 
TTACGACTTC TCTTTGAAAT ACATCATACT AAAGAACAAG GGGACATTGT TGTCTTTCTG 
GCCTGTGAAG AAGAGATAAA AAGGGCATAT GAGCATATCA AACAGGAAGC ATTGCACATG 
AATCCAGAAT TGGGTGAACT GGTACCCATT GCTTTGTACC CCAATCAAAG CATTTCAGAC 
TACATACCTT ATGAGGAACT GGATGACAAC AGCAAAAGCC CAAAAAGAAA AGTGGTATTG 
ACGACCAGCC TTGGCGAGTC TCTCATCTGG ATGAAAAATA TCCTCTTTGT CATTGATGTT 
GGTATAGAGA GGAGGAAGGT ATATAATACC AGAATCCGCG CAGAATCACA TGTCACGCAA 
CCAATAAGTA AGGTCCGGGC TGAGATGAGG AAGCATATAC TGAGCTCATA TTCTAAAGGA 
AAACTATTTT GCTTGTACCC CGAGGACTTT GCACATGAGA TGAAACCATT CCTGACTGCA 
AAAGTGGAAG AGTGCAATTT GATTTCAATG GTTCTGTTTC TGAAGAGAAT GGACATTGCG 
GGGCTCGCTC ACTGTGACTT CATCAACAGA CCAGACCCTG AAAGTTTAAT GCAAGCACTG 
GAAGATCTGG ATTATTTGGC AGCACTTGAC AATGATGGAA ATCTTTCTGA ATTTGGAATA 
ATTATGTCTG AGTTTCCACT CGATCCACAG CTGTCAAAAT CCATTTTAGC TGCCTGCGAA 
TTCGACTGCG TGGATGAAAT GCTCACACTG GCTGCTATGG TTACAGCCCC GAATTGTTTT 
ATCGATTCAT CCCCTGAAGC CGAAGAGCTG ATTTTAATTC GTAAAGGAAA GTTTTCTCAT 
CCAGAGGGGG ACCACTTCAC TCTTATAAAT ATCTACAATG AATATGAGCA GATGAAGAGA 
AGCAATGCTA GCCAGTACGA TGTTGAAAAA TGGTGTCAGG ATCACTGCCT AAGTTTCCTG 
GCAATGGAGA TGGCGCGTGC CATAAGAAAT GAGCTCGCTG ACATTATGAG GCGCATAGAG 
CTTCCACTGA CAGCGCCTGC ATTTGGCTCT GAGGAGAATG TAATAAACAT TAAAAAGTCA 
CTTTTGTCAG GATACTTCAT GCAAATTGCT CGTGATGTTG ATGGTTTGGG AAACTACATT 
ATGCTGACAC ACAAGCAGGT GGGACAACTC CATCCTGATT CTGGCTTCAA CGGCGCCAAG 
GTTCCAGAAT GGGTTCTCTA CCACGAATTT AGCGTCTCTG AGAGAAGCTG CATCAGGATA 
GTCTCAGAGA TCTCACCAAA CATGTTCATG GAGTTCGTTC CCCCGTACTA CTTCAGCAAC 
CTGCCCCCTA GTGAGACTAA AGAGCTCCTG CAGCAGCACC AGACTGTGCC CAGTTCCCCG 
GCTGAAGAGC CCGGAGAAGA GGAGCCCCTA CCTGATGCCG ATGATGAAGG TGCAACAGAA 
CAAAGATGCA TTATACAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx32 ( XM_031906149.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031762009.1 Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X3, mRNA.

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CloneID	OXa62622
Clone ID Related Accession (Same CDS sequence)	XM_004912720.4 , XM_031906149.1 , XM_002932650.5
Accession Version	XM_031906149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGG CTATATTGTG CCGTTCGAAA ACTGCTGTAC AAGCGAGACA ATTTTTAAGG 
TATTGCACGG ACGAGATGTT GCTGAGGGAA ATGATGTCCA GCCCCCTTCT GTCCAGTTAT 
GGAGTCGTCA TTATCGATGA TGTCTATGAA AGATCAGTTT CTACTGATGT GCTGCTCAGT 
TGCCTTAAGG TCATTGCAGT CTCAAGACCT GAACTGAAAG TTGTGATAAT TACCTGCCCT 
GCTCTGTCTG GGACCTTGGT TTCTTATTAT GGAAATGCTC CACTTGTAGA AACAGAAAAC 
ATGCACTCTG TAGAGTCGGT TTACACCACA AGCATTCCAA GAGACTACTT CCACTCAACT 
TTACGACTTC TCTTTGAAAT ACATCATACT AAAGAACAAG GGGACATTGT TGTCTTTCTG 
GCCTGTGAAG AAGAGATAAA AAGGGCATAT GAGCATATCA AACAGGAAGC ATTGCACATG 
AATCCAGAAT TGGGTGAACT GGTACCCATT GCTTTGTACC CCAATCAAAG CATTTCAGAC 
TACATACCTT ATGAGGAACT GGATGACAAC AGCAAAAGCC CAAAAAGAAA AGTGGTATTG 
ACGACCAGCC TTGGCGAGTC TCTCATCTGG ATGAAAAATA TCCTCTTTGT CATTGATGTT 
GGTATAGAGA GGAGGAAGGT ATATAATACC AGAATCCGCG CAGAATCACA TGTCACGCAA 
CCAATAAGTA AGGTCCGGGC TGAGATGAGG AAGCATATAC TGAGCTCATA TTCTAAAGGA 
AAACTATTTT GCTTGTACCC CGAGGACTTT GCACATGAGA TGAAACCATT CCTGACTGCA 
AAAGTGGAAG AGTGCAATTT GATTTCAATG GTTCTGTTTC TGAAGAGAAT GGACATTGCG 
GGGCTCGCTC ACTGTGACTT CATCAACAGA CCAGACCCTG AAAGTTTAAT GCAAGCACTG 
GAAGATCTGG ATTATTTGGC AGCACTTGAC AATGATGGAA ATCTTTCTGA ATTTGGAATA 
ATTATGTCTG AGTTTCCACT CGATCCACAG CTGTCAAAAT CCATTTTAGC TGCCTGCGAA 
TTCGACTGCG TGGATGAAAT GCTCACACTG GCTGCTATGG TTACAGCCCC GAATTGTTTT 
ATCGATTCAT CCCCTGAAGC CGAAGAGCTG ATTTTAATTC GTAAAGGAAA GTTTTCTCAT 
CCAGAGGGGG ACCACTTCAC TCTTATAAAT ATCTACAATG AATATGAGCA GATGAAGAGA 
AGCAATGCTA GCCAGTACGA TGTTGAAAAA TGGTGTCAGG ATCACTGCCT AAGTTTCCTG 
GCAATGGAGA TGGCGCGTGC CATAAGAAAT GAGCTCGCTG ACATTATGAG GCGCATAGAG 
CTTCCACTGA CAGCGCCTGC ATTTGGCTCT GAGGAGAATG TAATAAACAT TAAAAAGTCA 
CTTTTGTCAG GATACTTCAT GCAAATTGCT CGTGATGTTG ATGGTTTGGG AAACTACATT 
ATGCTGACAC ACAAGCAGGT GGGACAACTC CATCCTGATT CTGGCTTCAA CGGCGCCAAG 
GTTCCAGAAT GGGTTCTCTA CCACGAATTT AGCGTCTCTG AGAGAAGCTG CATCAGGATA 
GTCTCAGAGA TCTCACCAAA CATGTTCATG GAGTTCGTTC CCCCGTACTA CTTCAGCAAC 
CTGCCCCCTA GTGAGACTAA AGAGCTCCTG CAGCAGCACC AGACTGTGCC CAGTTCCCCG 
GCTGAAGAGC CCGGAGAAGA GGAGCCCCTA CCTGATGCCG ATGATGAAGG TGCAACAGAA 
CAAAGATGCA TTATACAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031762009.1
CDS	835..2655
Translation	MKLAILCRSKTAVQARQFLRYCTDEMLLREMMSSPLLSSYGVVIIDDVYERSVSTDVLLSCLKVIAVSRPELKVVIITCPALSGTLVSYYGNAPLVETENMHSVESVYTTSIPRDYFHSTLRLLFEIHHTKEQGDIVVFLACEEEIKRAYEHIKQEALHMNPELGELVPIALYPNQSISDYIPYEELDDNSKSPKRKVVLTTSLGESLIWMKNILFVIDVGIERRKVYNTRIRAESHVTQPISKVRAEMRKHILSSYSKGKLFCLYPEDFAHEMKPFLTAKVEECNLISMVLFLKRMDIAGLAHCDFINRPDPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILAACEFDCVDEMLTLAAMVTAPNCFIDSSPEAEELILIRKGKFSHPEGDHFTLINIYNEYEQMKRSNASQYDVEKWCQDHCLSFLAMEMARAIRNELADIMRRIELPLTAPAFGSEENVINIKKSLLSGYFMQIARDVDGLGNYIMLTHKQVGQLHPDSGFNGAKVPEWVLYHEFSVSERSCIRIVSEISPNMFMEFVPPYYFSNLPPSETKELLQQHQTVPSSPAEEPGEEEPLPDADDEGATEQRCIIQ

Target ORF information:

RefSeq Version	XM_031906149.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906149.1

ORF Insert Sequence:

ATGAAGTTGG CTATATTGTG CCGTTCGAAA ACTGCTGTAC AAGCGAGACA ATTTTTAAGG 
TATTGCACGG ACGAGATGTT GCTGAGGGAA ATGATGTCCA GCCCCCTTCT GTCCAGTTAT 
GGAGTCGTCA TTATCGATGA TGTCTATGAA AGATCAGTTT CTACTGATGT GCTGCTCAGT 
TGCCTTAAGG TCATTGCAGT CTCAAGACCT GAACTGAAAG TTGTGATAAT TACCTGCCCT 
GCTCTGTCTG GGACCTTGGT TTCTTATTAT GGAAATGCTC CACTTGTAGA AACAGAAAAC 
ATGCACTCTG TAGAGTCGGT TTACACCACA AGCATTCCAA GAGACTACTT CCACTCAACT 
TTACGACTTC TCTTTGAAAT ACATCATACT AAAGAACAAG GGGACATTGT TGTCTTTCTG 
GCCTGTGAAG AAGAGATAAA AAGGGCATAT GAGCATATCA AACAGGAAGC ATTGCACATG 
AATCCAGAAT TGGGTGAACT GGTACCCATT GCTTTGTACC CCAATCAAAG CATTTCAGAC 
TACATACCTT ATGAGGAACT GGATGACAAC AGCAAAAGCC CAAAAAGAAA AGTGGTATTG 
ACGACCAGCC TTGGCGAGTC TCTCATCTGG ATGAAAAATA TCCTCTTTGT CATTGATGTT 
GGTATAGAGA GGAGGAAGGT ATATAATACC AGAATCCGCG CAGAATCACA TGTCACGCAA 
CCAATAAGTA AGGTCCGGGC TGAGATGAGG AAGCATATAC TGAGCTCATA TTCTAAAGGA 
AAACTATTTT GCTTGTACCC CGAGGACTTT GCACATGAGA TGAAACCATT CCTGACTGCA 
AAAGTGGAAG AGTGCAATTT GATTTCAATG GTTCTGTTTC TGAAGAGAAT GGACATTGCG 
GGGCTCGCTC ACTGTGACTT CATCAACAGA CCAGACCCTG AAAGTTTAAT GCAAGCACTG 
GAAGATCTGG ATTATTTGGC AGCACTTGAC AATGATGGAA ATCTTTCTGA ATTTGGAATA 
ATTATGTCTG AGTTTCCACT CGATCCACAG CTGTCAAAAT CCATTTTAGC TGCCTGCGAA 
TTCGACTGCG TGGATGAAAT GCTCACACTG GCTGCTATGG TTACAGCCCC GAATTGTTTT 
ATCGATTCAT CCCCTGAAGC CGAAGAGCTG ATTTTAATTC GTAAAGGAAA GTTTTCTCAT 
CCAGAGGGGG ACCACTTCAC TCTTATAAAT ATCTACAATG AATATGAGCA GATGAAGAGA 
AGCAATGCTA GCCAGTACGA TGTTGAAAAA TGGTGTCAGG ATCACTGCCT AAGTTTCCTG 
GCAATGGAGA TGGCGCGTGC CATAAGAAAT GAGCTCGCTG ACATTATGAG GCGCATAGAG 
CTTCCACTGA CAGCGCCTGC ATTTGGCTCT GAGGAGAATG TAATAAACAT TAAAAAGTCA 
CTTTTGTCAG GATACTTCAT GCAAATTGCT CGTGATGTTG ATGGTTTGGG AAACTACATT 
ATGCTGACAC ACAAGCAGGT GGGACAACTC CATCCTGATT CTGGCTTCAA CGGCGCCAAG 
GTTCCAGAAT GGGTTCTCTA CCACGAATTT AGCGTCTCTG AGAGAAGCTG CATCAGGATA 
GTCTCAGAGA TCTCACCAAA CATGTTCATG GAGTTCGTTC CCCCGTACTA CTTCAGCAAC 
CTGCCCCCTA GTGAGACTAA AGAGCTCCTG CAGCAGCACC AGACTGTGCC CAGTTCCCCG 
GCTGAAGAGC CCGGAGAAGA GGAGCCCCTA CCTGATGCCG ATGATGAAGG TGCAACAGAA 
CAAAGATGCA TTATACAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx32 ( XM_002932650.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932696.3 Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa62622
Clone ID Related Accession (Same CDS sequence)	XM_004912720.4 , XM_031906149.1 , XM_002932650.5
Accession Version	XM_002932650.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932650.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTGG CTATATTGTG CCGTTCGAAA ACTGCTGTAC AAGCGAGACA ATTTTTAAGG 
TATTGCACGG ACGAGATGTT GCTGAGGGAA ATGATGTCCA GCCCCCTTCT GTCCAGTTAT 
GGAGTCGTCA TTATCGATGA TGTCTATGAA AGATCAGTTT CTACTGATGT GCTGCTCAGT 
TGCCTTAAGG TCATTGCAGT CTCAAGACCT GAACTGAAAG TTGTGATAAT TACCTGCCCT 
GCTCTGTCTG GGACCTTGGT TTCTTATTAT GGAAATGCTC CACTTGTAGA AACAGAAAAC 
ATGCACTCTG TAGAGTCGGT TTACACCACA AGCATTCCAA GAGACTACTT CCACTCAACT 
TTACGACTTC TCTTTGAAAT ACATCATACT AAAGAACAAG GGGACATTGT TGTCTTTCTG 
GCCTGTGAAG AAGAGATAAA AAGGGCATAT GAGCATATCA AACAGGAAGC ATTGCACATG 
AATCCAGAAT TGGGTGAACT GGTACCCATT GCTTTGTACC CCAATCAAAG CATTTCAGAC 
TACATACCTT ATGAGGAACT GGATGACAAC AGCAAAAGCC CAAAAAGAAA AGTGGTATTG 
ACGACCAGCC TTGGCGAGTC TCTCATCTGG ATGAAAAATA TCCTCTTTGT CATTGATGTT 
GGTATAGAGA GGAGGAAGGT ATATAATACC AGAATCCGCG CAGAATCACA TGTCACGCAA 
CCAATAAGTA AGGTCCGGGC TGAGATGAGG AAGCATATAC TGAGCTCATA TTCTAAAGGA 
AAACTATTTT GCTTGTACCC CGAGGACTTT GCACATGAGA TGAAACCATT CCTGACTGCA 
AAAGTGGAAG AGTGCAATTT GATTTCAATG GTTCTGTTTC TGAAGAGAAT GGACATTGCG 
GGGCTCGCTC ACTGTGACTT CATCAACAGA CCAGACCCTG AAAGTTTAAT GCAAGCACTG 
GAAGATCTGG ATTATTTGGC AGCACTTGAC AATGATGGAA ATCTTTCTGA ATTTGGAATA 
ATTATGTCTG AGTTTCCACT CGATCCACAG CTGTCAAAAT CCATTTTAGC TGCCTGCGAA 
TTCGACTGCG TGGATGAAAT GCTCACACTG GCTGCTATGG TTACAGCCCC GAATTGTTTT 
ATCGATTCAT CCCCTGAAGC CGAAGAGCTG ATTTTAATTC GTAAAGGAAA GTTTTCTCAT 
CCAGAGGGGG ACCACTTCAC TCTTATAAAT ATCTACAATG AATATGAGCA GATGAAGAGA 
AGCAATGCTA GCCAGTACGA TGTTGAAAAA TGGTGTCAGG ATCACTGCCT AAGTTTCCTG 
GCAATGGAGA TGGCGCGTGC CATAAGAAAT GAGCTCGCTG ACATTATGAG GCGCATAGAG 
CTTCCACTGA CAGCGCCTGC ATTTGGCTCT GAGGAGAATG TAATAAACAT TAAAAAGTCA 
CTTTTGTCAG GATACTTCAT GCAAATTGCT CGTGATGTTG ATGGTTTGGG AAACTACATT 
ATGCTGACAC ACAAGCAGGT GGGACAACTC CATCCTGATT CTGGCTTCAA CGGCGCCAAG 
GTTCCAGAAT GGGTTCTCTA CCACGAATTT AGCGTCTCTG AGAGAAGCTG CATCAGGATA 
GTCTCAGAGA TCTCACCAAA CATGTTCATG GAGTTCGTTC CCCCGTACTA CTTCAGCAAC 
CTGCCCCCTA GTGAGACTAA AGAGCTCCTG CAGCAGCACC AGACTGTGCC CAGTTCCCCG 
GCTGAAGAGC CCGGAGAAGA GGAGCCCCTA CCTGATGCCG ATGATGAAGG TGCAACAGAA 
CAAAGATGCA TTATACAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932696.3
CDS	731..2551
Translation	MKLAILCRSKTAVQARQFLRYCTDEMLLREMMSSPLLSSYGVVIIDDVYERSVSTDVLLSCLKVIAVSRPELKVVIITCPALSGTLVSYYGNAPLVETENMHSVESVYTTSIPRDYFHSTLRLLFEIHHTKEQGDIVVFLACEEEIKRAYEHIKQEALHMNPELGELVPIALYPNQSISDYIPYEELDDNSKSPKRKVVLTTSLGESLIWMKNILFVIDVGIERRKVYNTRIRAESHVTQPISKVRAEMRKHILSSYSKGKLFCLYPEDFAHEMKPFLTAKVEECNLISMVLFLKRMDIAGLAHCDFINRPDPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILAACEFDCVDEMLTLAAMVTAPNCFIDSSPEAEELILIRKGKFSHPEGDHFTLINIYNEYEQMKRSNASQYDVEKWCQDHCLSFLAMEMARAIRNELADIMRRIELPLTAPAFGSEENVINIKKSLLSGYFMQIARDVDGLGNYIMLTHKQVGQLHPDSGFNGAKVPEWVLYHEFSVSERSCIRIVSEISPNMFMEFVPPYYFSNLPPSETKELLQQHQTVPSSPAEEPGEEEPLPDADDEGATEQRCIIQ

Target ORF information:

RefSeq Version	XM_002932650.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932650.5

ORF Insert Sequence:

ATGAAGTTGG CTATATTGTG CCGTTCGAAA ACTGCTGTAC AAGCGAGACA ATTTTTAAGG 
TATTGCACGG ACGAGATGTT GCTGAGGGAA ATGATGTCCA GCCCCCTTCT GTCCAGTTAT 
GGAGTCGTCA TTATCGATGA TGTCTATGAA AGATCAGTTT CTACTGATGT GCTGCTCAGT 
TGCCTTAAGG TCATTGCAGT CTCAAGACCT GAACTGAAAG TTGTGATAAT TACCTGCCCT 
GCTCTGTCTG GGACCTTGGT TTCTTATTAT GGAAATGCTC CACTTGTAGA AACAGAAAAC 
ATGCACTCTG TAGAGTCGGT TTACACCACA AGCATTCCAA GAGACTACTT CCACTCAACT 
TTACGACTTC TCTTTGAAAT ACATCATACT AAAGAACAAG GGGACATTGT TGTCTTTCTG 
GCCTGTGAAG AAGAGATAAA AAGGGCATAT GAGCATATCA AACAGGAAGC ATTGCACATG 
AATCCAGAAT TGGGTGAACT GGTACCCATT GCTTTGTACC CCAATCAAAG CATTTCAGAC 
TACATACCTT ATGAGGAACT GGATGACAAC AGCAAAAGCC CAAAAAGAAA AGTGGTATTG 
ACGACCAGCC TTGGCGAGTC TCTCATCTGG ATGAAAAATA TCCTCTTTGT CATTGATGTT 
GGTATAGAGA GGAGGAAGGT ATATAATACC AGAATCCGCG CAGAATCACA TGTCACGCAA 
CCAATAAGTA AGGTCCGGGC TGAGATGAGG AAGCATATAC TGAGCTCATA TTCTAAAGGA 
AAACTATTTT GCTTGTACCC CGAGGACTTT GCACATGAGA TGAAACCATT CCTGACTGCA 
AAAGTGGAAG AGTGCAATTT GATTTCAATG GTTCTGTTTC TGAAGAGAAT GGACATTGCG 
GGGCTCGCTC ACTGTGACTT CATCAACAGA CCAGACCCTG AAAGTTTAAT GCAAGCACTG 
GAAGATCTGG ATTATTTGGC AGCACTTGAC AATGATGGAA ATCTTTCTGA ATTTGGAATA 
ATTATGTCTG AGTTTCCACT CGATCCACAG CTGTCAAAAT CCATTTTAGC TGCCTGCGAA 
TTCGACTGCG TGGATGAAAT GCTCACACTG GCTGCTATGG TTACAGCCCC GAATTGTTTT 
ATCGATTCAT CCCCTGAAGC CGAAGAGCTG ATTTTAATTC GTAAAGGAAA GTTTTCTCAT 
CCAGAGGGGG ACCACTTCAC TCTTATAAAT ATCTACAATG AATATGAGCA GATGAAGAGA 
AGCAATGCTA GCCAGTACGA TGTTGAAAAA TGGTGTCAGG ATCACTGCCT AAGTTTCCTG 
GCAATGGAGA TGGCGCGTGC CATAAGAAAT GAGCTCGCTG ACATTATGAG GCGCATAGAG 
CTTCCACTGA CAGCGCCTGC ATTTGGCTCT GAGGAGAATG TAATAAACAT TAAAAAGTCA 
CTTTTGTCAG GATACTTCAT GCAAATTGCT CGTGATGTTG ATGGTTTGGG AAACTACATT 
ATGCTGACAC ACAAGCAGGT GGGACAACTC CATCCTGATT CTGGCTTCAA CGGCGCCAAG 
GTTCCAGAAT GGGTTCTCTA CCACGAATTT AGCGTCTCTG AGAGAAGCTG CATCAGGATA 
GTCTCAGAGA TCTCACCAAA CATGTTCATG GAGTTCGTTC CCCCGTACTA CTTCAGCAAC 
CTGCCCCCTA GTGAGACTAA AGAGCTCCTG CAGCAGCACC AGACTGTGCC CAGTTCCCCG 
GCTGAAGAGC CCGGAGAAGA GGAGCCCCTA CCTGATGCCG ATGATGAAGG TGCAACAGAA 
CAAAGATGCA TTATACAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhx32 ( XM_031906150.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031762010.1 Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X4, mRNA.

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CloneID	OXa62623
Clone ID Related Accession (Same CDS sequence)	XM_031906150.1
Accession Version	XM_031906150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTTT CACTAGAGGC CTATGAACAG GACGGCGATG AACTCATTTC CGAAGAGAAA 
AGCTGTTGCC TCGATGTTAC AGACTCAAGT GATGTGGAAG GAGATGAACT TAATGACTTG 
GAACTAAATC CATTTGATGG TTTGCCTTAC TCATCTCGTT TTTACAAACT CTTAAAAGAA 
AGAGAAGATC TTCCCATATG GAAAGCCAAA TATGACTTTT TGGAGCGATT ATCTAACAGC 
CAAGTTGTGG TTGTGTCGGC GGGACCTAAG AGCGGAAAGA GTTCACAAAT TCCTCAATGG 
TGCGCAGAAT ACTGCTTAGC AGGGCACTAT CAACATGGGG CAGTTGTGTG TACGCAGGCG 
CACAAGCAGA CGGCAGTATG GCTGGCCATG AGAGTGGCAG ACGAAATGGA TGTCAATATA 
GGACATGAAG TTGGCTATAT TGTGCCGTTC GAAAACTGCT GTACAAGCGA GACAATTTTC 
AGGTATTGCA CGGACGAGAT GTTGCTGAGG GAAATGATGT CCAGCCCCCT TCTGTCCAGT 
TATGGAGTCG TCATTATCGA TGATGTCTAT GAAAGATCAG TTTCTACTGA TGTGCTGCTC 
AGTTGCCTTA AGGTCATTGC AGTCTCAAGA CCTGAACTGA AAGTTGTGAT AATTACCTGC 
CCTGCTCTGT CTGGGACCTT GGTTTCTTAT TATGGAAATG CTCCACTTGT AGAAACAGAA 
AACATGCACT CTGTAGAGTC GGTTTACACC ACAAGCATTC CAAGAGACTA CTTCCACTCA 
ACTTTACGAC TTCTCTTTGA AATACATCAT ACTAAAGAAC AAGGGGACAT TGTTGTCTTT 
CTGGCCTGTG AAGAAGAGAT AAAAAGGGCA TATGAGCATA TCAAACAGGA AGCATTGCAC 
ATGAATCCAG AATTGGGTGA ACTGGTACCC ATTGCTTTGT ACCCCAATCA AAGCATTTCA 
GACTACATAC CTTATGAGGA ACTGGATGAC AACAGCAAAA GCCCAAAAAG AAAAGTGGTA 
TTGACGACCA GCCTTGGCGA GTCTCTCATC TGGATGAAAA ATATCCTCTT TGTCATTGAT 
GTTGGTATAG AGAGGAGGAA GGTATATAAT ACCAGAATCC GCGCAGAATC ACATGTCACG 
CAACCAATAA GTAAGGTCCG GGCTGAGATG AGGAAGCATA TACTGAGCTC ATATTCTAAA 
GGAAAACTAT TTTGCTTGTA CCCCGAGGAC TTTGCACATG AGATGAAACC ATTCCTGACT 
GCAAAAGTGG AAGAGTGCAA TTTGATTTCA ATGGTTCTGT TTCTGAAGAG AATGGACATT 
GCGGGGCTCG CTCACTGTGA CTTCATCAAC AGACCAGACC CTGAAAGTTT AATGCAAGCA 
CTGGAAGATC TGGATTATTT GGCAGCACTT GACAATGATG GAAATCTTTC TGAATTTGGA 
ATAATTATGT CTGAGTTTCC ACTCGATCCA CAGCTGTCAA AATCCATTTT AGCTGCCTGC 
GAATTCGACT GCGTGGATGA AATGCTCACA CTGGCTGCTA TGGTTACAGC CCCGAATTGT 
TTTATCGATT CATCCCCTGA AGCCGAAGAG CTGATTTTAA TTCGTAAAGG AAAGTTTTCT 
CATCCAGAGG GGGACCACTT CACTCTTATA AATATCTACA ATGAATATGA GCAGATGAAG 
AGAAGCAATG CTAGCCAGTA CGATGTTGAA AAATGGTGTC AGGATCACTG CCTAAGTTTC 
CTGGCAATGG AGATGGCGCG TGCCATAAGA AATGAGCTCG CTGACATTAT GAGGCGCATA 
GAGCTTCCAC TGACAGCGCC TGCATTTGGC TCTGAGGAGA ATGTAATAAA CATTAAAAAG 
TCACTTTTGT CAGGATACTT CATGCAAATT GCTCGTGATG TTGATGGTTT GGGAAACTAC 
ATTATGCTGA CACACAAGCA GGTGGGACAA CTCCATCCTG ATTCTGGCTT CAACGGCGCC 
AAGGTTCCAG AATGGGTTCT CTACCACGAA TTTAGCGTCT CTGAGAGAAG CTGCATCAGG 
ATAGTCTCAG AGATCTCACC AAACATGTTC ATGGAGTTCG TTCCCCCGTA CTACTTCAGC 
AACCTGCCCC CTAGTGAGAC TAAAGAGCTC CTGCAGCAGC ACCAGACTGT GCCCAGTTCC 
CCGGCTGAAG AGCCCGGAGA AGAGGAGCCC CTACCTGATG CCGATGATGA AGGTGCAACA 
GAACAAAGAT GCATTATACA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031762010.1
CDS	307..2550
Translation	MALSLEAYEQDGDELISEEKSCCLDVTDSSDVEGDELNDLELNPFDGLPYSSRFYKLLKEREDLPIWKAKYDFLERLSNSQVVVVSAGPKSGKSSQIPQWCAEYCLAGHYQHGAVVCTQAHKQTAVWLAMRVADEMDVNIGHEVGYIVPFENCCTSETIFRYCTDEMLLREMMSSPLLSSYGVVIIDDVYERSVSTDVLLSCLKVIAVSRPELKVVIITCPALSGTLVSYYGNAPLVETENMHSVESVYTTSIPRDYFHSTLRLLFEIHHTKEQGDIVVFLACEEEIKRAYEHIKQEALHMNPELGELVPIALYPNQSISDYIPYEELDDNSKSPKRKVVLTTSLGESLIWMKNILFVIDVGIERRKVYNTRIRAESHVTQPISKVRAEMRKHILSSYSKGKLFCLYPEDFAHEMKPFLTAKVEECNLISMVLFLKRMDIAGLAHCDFINRPDPESLMQALEDLDYLAALDNDGNLSEFGIIMSEFPLDPQLSKSILAACEFDCVDEMLTLAAMVTAPNCFIDSSPEAEELILIRKGKFSHPEGDHFTLINIYNEYEQMKRSNASQYDVEKWCQDHCLSFLAMEMARAIRNELADIMRRIELPLTAPAFGSEENVINIKKSLLSGYFMQIARDVDGLGNYIMLTHKQVGQLHPDSGFNGAKVPEWVLYHEFSVSERSCIRIVSEISPNMFMEFVPPYYFSNLPPSETKELLQQHQTVPSSPAEEPGEEEPLPDADDEGATEQRCIIQ

Target ORF information:

RefSeq Version	XM_031906150.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAH-box helicase 32 (putative) (dhx32), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906150.1

ORF Insert Sequence:

ATGGCTCTTT CACTAGAGGC CTATGAACAG GACGGCGATG AACTCATTTC CGAAGAGAAA 
AGCTGTTGCC TCGATGTTAC AGACTCAAGT GATGTGGAAG GAGATGAACT TAATGACTTG 
GAACTAAATC CATTTGATGG TTTGCCTTAC TCATCTCGTT TTTACAAACT CTTAAAAGAA 
AGAGAAGATC TTCCCATATG GAAAGCCAAA TATGACTTTT TGGAGCGATT ATCTAACAGC 
CAAGTTGTGG TTGTGTCGGC GGGACCTAAG AGCGGAAAGA GTTCACAAAT TCCTCAATGG 
TGCGCAGAAT ACTGCTTAGC AGGGCACTAT CAACATGGGG CAGTTGTGTG TACGCAGGCG 
CACAAGCAGA CGGCAGTATG GCTGGCCATG AGAGTGGCAG ACGAAATGGA TGTCAATATA 
GGACATGAAG TTGGCTATAT TGTGCCGTTC GAAAACTGCT GTACAAGCGA GACAATTTTC 
AGGTATTGCA CGGACGAGAT GTTGCTGAGG GAAATGATGT CCAGCCCCCT TCTGTCCAGT 
TATGGAGTCG TCATTATCGA TGATGTCTAT GAAAGATCAG TTTCTACTGA TGTGCTGCTC 
AGTTGCCTTA AGGTCATTGC AGTCTCAAGA CCTGAACTGA AAGTTGTGAT AATTACCTGC 
CCTGCTCTGT CTGGGACCTT GGTTTCTTAT TATGGAAATG CTCCACTTGT AGAAACAGAA 
AACATGCACT CTGTAGAGTC GGTTTACACC ACAAGCATTC CAAGAGACTA CTTCCACTCA 
ACTTTACGAC TTCTCTTTGA AATACATCAT ACTAAAGAAC AAGGGGACAT TGTTGTCTTT 
CTGGCCTGTG AAGAAGAGAT AAAAAGGGCA TATGAGCATA TCAAACAGGA AGCATTGCAC 
ATGAATCCAG AATTGGGTGA ACTGGTACCC ATTGCTTTGT ACCCCAATCA AAGCATTTCA 
GACTACATAC CTTATGAGGA ACTGGATGAC AACAGCAAAA GCCCAAAAAG AAAAGTGGTA 
TTGACGACCA GCCTTGGCGA GTCTCTCATC TGGATGAAAA ATATCCTCTT TGTCATTGAT 
GTTGGTATAG AGAGGAGGAA GGTATATAAT ACCAGAATCC GCGCAGAATC ACATGTCACG 
CAACCAATAA GTAAGGTCCG GGCTGAGATG AGGAAGCATA TACTGAGCTC ATATTCTAAA 
GGAAAACTAT TTTGCTTGTA CCCCGAGGAC TTTGCACATG AGATGAAACC ATTCCTGACT 
GCAAAAGTGG AAGAGTGCAA TTTGATTTCA ATGGTTCTGT TTCTGAAGAG AATGGACATT 
GCGGGGCTCG CTCACTGTGA CTTCATCAAC AGACCAGACC CTGAAAGTTT AATGCAAGCA 
CTGGAAGATC TGGATTATTT GGCAGCACTT GACAATGATG GAAATCTTTC TGAATTTGGA 
ATAATTATGT CTGAGTTTCC ACTCGATCCA CAGCTGTCAA AATCCATTTT AGCTGCCTGC 
GAATTCGACT GCGTGGATGA AATGCTCACA CTGGCTGCTA TGGTTACAGC CCCGAATTGT 
TTTATCGATT CATCCCCTGA AGCCGAAGAG CTGATTTTAA TTCGTAAAGG AAAGTTTTCT 
CATCCAGAGG GGGACCACTT CACTCTTATA AATATCTACA ATGAATATGA GCAGATGAAG 
AGAAGCAATG CTAGCCAGTA CGATGTTGAA AAATGGTGTC AGGATCACTG CCTAAGTTTC 
CTGGCAATGG AGATGGCGCG TGCCATAAGA AATGAGCTCG CTGACATTAT GAGGCGCATA 
GAGCTTCCAC TGACAGCGCC TGCATTTGGC TCTGAGGAGA ATGTAATAAA CATTAAAAAG 
TCACTTTTGT CAGGATACTT CATGCAAATT GCTCGTGATG TTGATGGTTT GGGAAACTAC 
ATTATGCTGA CACACAAGCA GGTGGGACAA CTCCATCCTG ATTCTGGCTT CAACGGCGCC 
AAGGTTCCAG AATGGGTTCT CTACCACGAA TTTAGCGTCT CTGAGAGAAG CTGCATCAGG 
ATAGTCTCAG AGATCTCACC AAACATGTTC ATGGAGTTCG TTCCCCCGTA CTACTTCAGC 
AACCTGCCCC CTAGTGAGAC TAAAGAGCTC CTGCAGCAGC ACCAGACTGT GCCCAGTTCC 
CCGGCTGAAG AGCCCGGAGA AGAGGAGCCC CTACCTGATG CCGATGATGA AGGTGCAACA 
GAACAAAGAT GCATTATACA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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dhx32 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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