col12a1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following col12a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col12a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa58277 XM_018094326.2
Latest version!
Xenopus tropicalis collagen, type XII, alpha 1 (col12a1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa10388 XM_002936200.2
Latest version!
Xenopus tropicalis collagen type XII alpha 1 chain (col12a1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa36220 XM_012963500.1
Latest version!
Xenopus tropicalis collagen type XII alpha 1 chain (col12a1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OXa36220 XM_018094326.1
Latest version!
Xenopus tropicalis collagen type XII alpha 1 chain (col12a1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OXa44710 XM_018094325.1
Latest version!
Xenopus tropicalis collagen type XII alpha 1 chain (col12a1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa58275 XM_031901995.1
Latest version!
Xenopus tropicalis collagen, type XII, alpha 1 (col12a1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa58275 XM_002936200.3
Latest version!
Xenopus tropicalis collagen, type XII, alpha 1 (col12a1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OXa58276 XM_018094325.2
Latest version!
Xenopus tropicalis collagen, type XII, alpha 1 (col12a1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa58277
    Clone ID Related Accession (Same CDS sequence) XM_018094326.2
    Accession Version XM_018094326.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XII) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGGCCTGGA ATGCAAAACT AAAGCTGCAG CTGATATAGT GTTGCTGGTG
    GATGGCTCTT GGAGTATCGG CCGCCCAAAT TTTAGGACCG TCAGGAGTTT TATAGCACGA
    CTGGTTGAAG TCTTTGATAT TGGCTCAGAC AGAGTGCAGA TTGCCTTGGC CCAATACAGT
    GGGGACCCTC GATCTGAATG GCAACTCAAT GCTCATAGCA CAAAGAAGTC TTTAATGGAT
    GCCGTTGCAA ACCTTCCTTA CAAGGGGGGT AACACTCTTA CAGGCATGGC TCTGAATTTC
    ATCTTGCAAA ATAACTTCAA GGCAGAGGCA GGCATGAGAG CAAAGTCAAA GAAAATTGGT
    GTCTTAATCA CTGATGGAAA GTCACAAGAT GACATTGTTG CACCTTCCAA AAAGTTACGA
    GACCAGGGAA TAGACTTATT TGCAATTGGT ATCAAAAATG CTGATGAAAA TGAACTGAGG
    CAGATTGGCT CAGATCCAGA TGAGACTTAC GTATTTAACG TTGCCGATTT TTCTTTGCTT
    GTCAATATTG TTGATGACCT CACTACCAAT CTATGTAACA GTGTGAAAGG CTCAGGAAAT
    GAACTGGAGC CACCTTCAGA CCTAGTAACA TCAGAGCCTA CACCACGGAG CTTCAGAGTC
    ACATGGAATC CACCTTCTCA CAGTGTAGAC CGATTTAGGG TGGAATATTA TCCAGTGTCT
    GGTGGTCGAC CTCTGGAGGT ATTTGTTGGC AGATCAGATA GAACTACGGT TTTGACTAAT
    TTAAAGCCAG AAACAGAGTA TGTTGTGAAA GTGTATTCTG TGTTAGAAGG AGAAAGCAGT
    GAACCATTAG AAGGCACAGA AACGACATTG CCCATCCCTT CAGTCAGAAA TCTTAACATA
    TATGATATAG GAACCAGAAC AATGAGAGTT CGATGGGAAG CTGCGCTTGG AGCTACTGGT
    TATTTATTGC AGTATGAGCC AGTAAACGCT ACTGTGCCTT CAACTGAAAA AGAGTTGAGA
    GTTGGTGCAT CGGTGACAGA TGTACTTCTG GAAGATTTAT TCCCCAACAC TGAGTACACC
    TTGACTGTAT ATGCACTTTT TGATGAGCTG ATCAGTGATC CTCTTACCTC ACAGGAAGTC
    ACTTTACCCA TTCAAGGCGT GGGGAACATT AGAGTGAGAG ATATCACTCA CAGCTCAATG
    AATGTCCTTT GGAGTGCTGC TCCTGGAAAT GTTCTCAAAT ATATAGTAAG ATATAAAACA
    GAGGAAGAAG AAGAATTCAA AGAGGTGGAA GTGGATGGAT CAAGGACAAG TACACGACTT
    ACTGACCTTT TCTCACAAAC TCTATATGAA ATCAGTTTAA CTGCAGTATA TGATGAAGGA
    GTATCTCAGC CACGTACTAC ACAAGGAACT ACAGCACCAG TACCAGCTCC AGCAAACCTC
    CGGTTTTCTG AAATAGCAGT AACAAGTTTC CGAGGTACTT GGGAGCATGG GGCTCCAGAT
    GTAGCTTTGT ACAGGATAAC GTGGGCACCA GTTGGAGCCA TAGAAAAAAA GAAAGAGACA
    ATTGTTGATG GTGATATAAA CACTTTGGTT TTTGATGAAC TGGAACCAGA CACAGAGTAT
    GAGGTTTCAG TAACTGCGAT TTACCCTGAC GAATCAGAGA GTGAAGATCT GATCGGTGTT
    GAAAGGACAT TGCCTGAAAT AATTTCTACA ACACCTGCCC TAAGGACTGG CCCTAGGAAT
    CTACAGGTGT TCAATGCAAC ACAGAATAGT TTGACTGTAC GATGGGATCG TGCTATGGGC
    GATGTGCAAA AATACAATAT CTATTATAAG CCTGTTTCTG CTGCTGGTGC TGAACAAATG
    ACAAGTGTTG GTGGAAAGCA GAACAGTGTT GTTCTTCAGC GACTGCAGCC AGACACTCCA
    TACAACATTG TTGTTTCATC AAGGTACATT GGAGATGAGG AAGGAGGAAA TATCTCAGGG
    AGAGGAAAAA CAAAGCCCTT AAATACAGTG AAGAATCTAA GAGTGTATGA CCCAACAACA
    AGCACTTTAA ATGTACGTTG GGAAGCTGCA GAAGGAAATG TGCGGCGGTA TAAAATTTAC
    TATGTACCAA CTGCTGGAGG ACCAGAGGAA ACGGTGACAG TGCCCGGAAA TACAATCTAT
    TCAGTTCTTA GGAATCTTCA GTCTGATACA TCATATACAG TAACTGTTGT TCCTGTCTAT
    TCTGAAGGAG AAGGAGGTCG TATGTCAGAA ACAGGAAAGA CTTTGGTGCG GGGAACAGTT
    CGAAATATTC AAGTATACAA CCCCACAACA AACACTTTGA ATGTTCGCTG GGAGCCTGCG
    CCTGGACAGG TCCAGCAATA TAAAGTCAAC TATGCTCCAC TTCTTGGAAC AAGGCCTACT
    GAGTCTGTTG TAGTTCCTGG CAATACTCAC AACGCGCTGC TGGAGCAACT TATTCCTGAC
    ACACAGTACT CTGTAAATGT TGTGGCTCTT TATGCAGATG GTGAGGGGGA TCCTAGCCCT
    GGTGAAGGAA AAACATTACC TCGCAGTGGG CCAAAAAATA TTAGAGTTTT TGGAGAGACC
    ACTAACAGTC TCTCTGTGAA CTGGGATCAT GCTGATGGAC CAGTCCAACA ATACAGAATC
    ATCTATTCAC CAACTGTTGG AGACCCCATT GATGAATATA CAACAGTGCC TGGTAGGAGA
    AATAATGTGG TACTGCAGCC CCTTTTGTCT GACACGCCAT ATAAAATTAC TGTGGTGGCA
    GTTTATGCTG ATGGTGATGG AGGACAACTG ACAGGAAAAG GAAGGACAGT GGGTTTGATT
    GCACCACGGA ACCTTCAAGT GTCTGATGAA TGGTACACAA GATTTAGGGT TTCCTGGGAT
    AGAATACCAT CTCCTGTTCT TGGATACAAG CTGGTTTATC AGCCACGTGA TTCAGATGAA
    TCAATGGAAG TATTTGTCGG GGATGTGAGC TCCTACACTT TGCAGAACCT GTCTCCTGGC
    ACCACATATG ATGTGAAAGT TTACGCTCAG TATGATACAG GATTAAGTGG GCCCCTATCA
    GGACCTGGGA CAACTTTATA CTTAAATGTA ACCGATTTGA CAAGCTATCA GGTTTCATGG
    GACTCGTTCT GTATAAGATG GACTCCTCAC CGATCAGCCA CTTCCTACAG ACTGAAACTG
    AATCCAGTTG ATGGATCTCG AGGTCAAGAA ATAACAGTAA CAGGATCAGA ATCATCACAC
    TGTTTTAATG GACTGAACCA AGATACACAG TACACAGCTA CTGTTTTTGT GCAAACACCT
    AACCTTGAAG GACGAGGAGT TTCAATAGAT GAGCGTACAT TGATCAAGCC TACAGAATCA
    CCAACGACAC CACCTGCTCC TCCTCCACCC CCTACAATTC CACCTGCTCG GGAGGTGTGC
    AAAGGGGCCA AAGCAGACAT AGTATTCCTC ATTGACAGTT CGTGGAGTAT TGGTGATGAT
    AACTACAATA AAGTCCGTCA GTTTATCTTC AATACTATTG GAGCATTCGA TGTAATCAAC
    CCTTCTGGAA TCCAGGTCTC TTTTGTGCAG TTCAGCGATG ATGCCCAGAC GGAGTTCAAG
    TTGAACACTT ATGCCGACAA ATCACAGGCT CTAGGAGCAC TTGCCAATAT CAGATACAAA
    GGAGGAAACA CAAAAACAGG AAAGGCTCTT AAATTTGTCC ACGAGAAAGT GATGACAGTT
    GAGAATGGAA TGAGAAGATC TGTTCCTAGG GTTCTGGTAG TGGTGACTGA TGGCAGATCT
    CAGGATGATG TTAAGAAATC AGCCTTGGAC ATTCAGCAAT CTGGTTTTAG TGTCTTTGTC
    ATTGGTGTGG CTGATGTGGA TTATAATGAG CTAAGAAACA TTGGCAGTAA ACCAAGCGAG
    CGCCATGTTT TTATAGTCGA TGACTTTGAT GCTTTTGAGA AGATTGAAGA TAACCTGATC
    AACTTTGTAT GTGAGACTGC AACATCCACT TGCCCACTTA TTTACCTGGA AGGATACACT
    TCACCAGGTT TCAGAATGCT GGAAGCATAT AACCTGACAG AGAAAACTTT TGCCTCAGTA
    CAAGGTGTCT CAATGCAACC AGGATCTTTC AACAGTTTCC CTGCATACAA CCTACATAAA
    GATGCCTTCA TTTCCCTGCC TACCTCAGAA TTGCACCCAG AAGGATTACC ACCTTCATAT
    ACGATTATAT TGCTATTCCG TCTGCTGCCA GAAACTCCCA ATGAGCCATT TTCTCTATGG
    CAAATAACAG ATAAAGGTTA CAAGCCACAA GTAGGAGTAA ATCTTGATCC ATCTAAGAAA
    ACACTGTCGT TCTTTAATAA GGGAGCTGCT GGGGATACTC AGACAGTAAC ATTTGATGGG
    AATGAAGTGA AGAAACTCTT TTATGGAAGC TTCCACAAGG TTCTCATTGT GGTTTCTCCA
    TCATACGTCA GAATTTATAT TGATTGTAGT GAAGTTGCTG AAAAAGAAAT CAAAGAAGCT
    GGCAATATTA CAGCAGAAGG ATATGAAGTA CTTGGGAAGA CATTAAAGGG TGATAAAAAA
    TCTGCACCCT TCCTGCTCCA GATGTTTGAC ATTGTGTGTA CTGTGACCTG GACAAGTCGA
    GATAGATGCT GTGATATTCC TGCTACAAGG GATGAAGCCA AATGTCCAGC TCTGCCCCAT
    TCCTGTACTT GCGCACAAGA CAGCGTTGGA GCACCAGGAC CCCCAGGCCC ATCCGGTATT
    CCCGGTAGTA AAGGACCACG GGGTGACCGA GGTTTGGATG GACCAGTTGG CCCACCAGGT
    CAGCGAGGAG AACAAGGTTT GCCTGGTCCA CAAGGACCAC CTGGACCTCA AGGACCACCT
    GGACTTTCCA TTCCTGGAGA ACCTGGTCGT CAAGGAATAA AAGGAGATGC CGGTGACCCA
    GGATTGCCAG GAAGAATGGG TTCCCCAGGG GTGACAGGAC CACCAGGGCC TATGGGTCCA
    CCAGGCACAA GGGGTTTCAC AGGAAAGGAT GGTGCAGTAG GACCCAGAGG ACCACCAGGA
    CCAATGGGAG CACCTGGACC TCAGGGAGTG CCAGGTCCAA GTGGGAAACC AGGAAAGCAA
    GGAGATAATG GATTGCCAGG TCCTCTTGGA CAGAAAGGAG ACAAGGGTGA GAGGGGAGAC
    ATGGCATCTC AGAATATGAT GAGATCAGTA GCAAGACAAG TCTGTGAACA GATGATAAAT
    AGTCAAATGA GCAGATTTAA TCAGATGTTG AACCAAATTC CAAATGATTA CTATTCCAAT
    CGCAATCAGC CTGGTCCTCC AGGTCCTCCC GGTCCTCCAG GCAACGCTGG CATGAGAGGA
    GAACCTGGAG CTGCTGGCAG ATCTGGATTC CCAGGAACAC CTGGTCCTCA AGGACCACCT
    GGAGAAAGAG GAATACCAGG AGAGAAAGGG GAGAGAGGGA ACCCAGGAAT TGGATCCCAA
    GGACCCAGGG GACTTCCAGG ACCTCCAGGA CCTCCAGGCG AATCCAGAAC AGGACCTCCA
    GGATCCTCAG GCTCCACAGG ACCTCCAGGA CCTTCAGGTC GCCCCGGTAA CCAAGGAATT
    AGAGGGCCTC CTGGTCCAGC TGGATACTGT GATGCTTCAA TGTGTGCAAG CATTCCTTAC
    AATGGGCAAG GATACCCAGA ACCTTATCAG CCTGAAAGTG GACCGCAGGA GCCAGAGAAT
    CAGCCTTACG TAGTACCTAT GCAGCAAGAG AGTCCAGATG AGGATTTTGA GGACTATGGA
    CATGAATATG CAGAACATAT GCGATGGAGG AGATCCTTAC CAAAGAAATT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949815.2
    CDS189..6062
    Translation

    Target ORF information:

    RefSeq Version XM_018094326.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XII, alpha 1 (col12a1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094326.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGGCCTGGA ATGCAAAACT AAAGCTGCAG CTGATATAGT GTTGCTGGTG
    GATGGCTCTT GGAGTATCGG CCGCCCAAAT TTTAGGACCG TCAGGAGTTT TATAGCACGA
    CTGGTTGAAG TCTTTGATAT TGGCTCAGAC AGAGTGCAGA TTGCCTTGGC CCAATACAGT
    GGGGACCCTC GATCTGAATG GCAACTCAAT GCTCATAGCA CAAAGAAGTC TTTAATGGAT
    GCCGTTGCAA ACCTTCCTTA CAAGGGGGGT AACACTCTTA CAGGCATGGC TCTGAATTTC
    ATCTTGCAAA ATAACTTCAA GGCAGAGGCA GGCATGAGAG CAAAGTCAAA GAAAATTGGT
    GTCTTAATCA CTGATGGAAA GTCACAAGAT GACATTGTTG CACCTTCCAA AAAGTTACGA
    GACCAGGGAA TAGACTTATT TGCAATTGGT ATCAAAAATG CTGATGAAAA TGAACTGAGG
    CAGATTGGCT CAGATCCAGA TGAGACTTAC GTATTTAACG TTGCCGATTT TTCTTTGCTT
    GTCAATATTG TTGATGACCT CACTACCAAT CTATGTAACA GTGTGAAAGG CTCAGGAAAT
    GAACTGGAGC CACCTTCAGA CCTAGTAACA TCAGAGCCTA CACCACGGAG CTTCAGAGTC
    ACATGGAATC CACCTTCTCA CAGTGTAGAC CGATTTAGGG TGGAATATTA TCCAGTGTCT
    GGTGGTCGAC CTCTGGAGGT ATTTGTTGGC AGATCAGATA GAACTACGGT TTTGACTAAT
    TTAAAGCCAG AAACAGAGTA TGTTGTGAAA GTGTATTCTG TGTTAGAAGG AGAAAGCAGT
    GAACCATTAG AAGGCACAGA AACGACATTG CCCATCCCTT CAGTCAGAAA TCTTAACATA
    TATGATATAG GAACCAGAAC AATGAGAGTT CGATGGGAAG CTGCGCTTGG AGCTACTGGT
    TATTTATTGC AGTATGAGCC AGTAAACGCT ACTGTGCCTT CAACTGAAAA AGAGTTGAGA
    GTTGGTGCAT CGGTGACAGA TGTACTTCTG GAAGATTTAT TCCCCAACAC TGAGTACACC
    TTGACTGTAT ATGCACTTTT TGATGAGCTG ATCAGTGATC CTCTTACCTC ACAGGAAGTC
    ACTTTACCCA TTCAAGGCGT GGGGAACATT AGAGTGAGAG ATATCACTCA CAGCTCAATG
    AATGTCCTTT GGAGTGCTGC TCCTGGAAAT GTTCTCAAAT ATATAGTAAG ATATAAAACA
    GAGGAAGAAG AAGAATTCAA AGAGGTGGAA GTGGATGGAT CAAGGACAAG TACACGACTT
    ACTGACCTTT TCTCACAAAC TCTATATGAA ATCAGTTTAA CTGCAGTATA TGATGAAGGA
    GTATCTCAGC CACGTACTAC ACAAGGAACT ACAGCACCAG TACCAGCTCC AGCAAACCTC
    CGGTTTTCTG AAATAGCAGT AACAAGTTTC CGAGGTACTT GGGAGCATGG GGCTCCAGAT
    GTAGCTTTGT ACAGGATAAC GTGGGCACCA GTTGGAGCCA TAGAAAAAAA GAAAGAGACA
    ATTGTTGATG GTGATATAAA CACTTTGGTT TTTGATGAAC TGGAACCAGA CACAGAGTAT
    GAGGTTTCAG TAACTGCGAT TTACCCTGAC GAATCAGAGA GTGAAGATCT GATCGGTGTT
    GAAAGGACAT TGCCTGAAAT AATTTCTACA ACACCTGCCC TAAGGACTGG CCCTAGGAAT
    CTACAGGTGT TCAATGCAAC ACAGAATAGT TTGACTGTAC GATGGGATCG TGCTATGGGC
    GATGTGCAAA AATACAATAT CTATTATAAG CCTGTTTCTG CTGCTGGTGC TGAACAAATG
    ACAAGTGTTG GTGGAAAGCA GAACAGTGTT GTTCTTCAGC GACTGCAGCC AGACACTCCA
    TACAACATTG TTGTTTCATC AAGGTACATT GGAGATGAGG AAGGAGGAAA TATCTCAGGG
    AGAGGAAAAA CAAAGCCCTT AAATACAGTG AAGAATCTAA GAGTGTATGA CCCAACAACA
    AGCACTTTAA ATGTACGTTG GGAAGCTGCA GAAGGAAATG TGCGGCGGTA TAAAATTTAC
    TATGTACCAA CTGCTGGAGG ACCAGAGGAA ACGGTGACAG TGCCCGGAAA TACAATCTAT
    TCAGTTCTTA GGAATCTTCA GTCTGATACA TCATATACAG TAACTGTTGT TCCTGTCTAT
    TCTGAAGGAG AAGGAGGTCG TATGTCAGAA ACAGGAAAGA CTTTGGTGCG GGGAACAGTT
    CGAAATATTC AAGTATACAA CCCCACAACA AACACTTTGA ATGTTCGCTG GGAGCCTGCG
    CCTGGACAGG TCCAGCAATA TAAAGTCAAC TATGCTCCAC TTCTTGGAAC AAGGCCTACT
    GAGTCTGTTG TAGTTCCTGG CAATACTCAC AACGCGCTGC TGGAGCAACT TATTCCTGAC
    ACACAGTACT CTGTAAATGT TGTGGCTCTT TATGCAGATG GTGAGGGGGA TCCTAGCCCT
    GGTGAAGGAA AAACATTACC TCGCAGTGGG CCAAAAAATA TTAGAGTTTT TGGAGAGACC
    ACTAACAGTC TCTCTGTGAA CTGGGATCAT GCTGATGGAC CAGTCCAACA ATACAGAATC
    ATCTATTCAC CAACTGTTGG AGACCCCATT GATGAATATA CAACAGTGCC TGGTAGGAGA
    AATAATGTGG TACTGCAGCC CCTTTTGTCT GACACGCCAT ATAAAATTAC TGTGGTGGCA
    GTTTATGCTG ATGGTGATGG AGGACAACTG ACAGGAAAAG GAAGGACAGT GGGTTTGATT
    GCACCACGGA ACCTTCAAGT GTCTGATGAA TGGTACACAA GATTTAGGGT TTCCTGGGAT
    AGAATACCAT CTCCTGTTCT TGGATACAAG CTGGTTTATC AGCCACGTGA TTCAGATGAA
    TCAATGGAAG TATTTGTCGG GGATGTGAGC TCCTACACTT TGCAGAACCT GTCTCCTGGC
    ACCACATATG ATGTGAAAGT TTACGCTCAG TATGATACAG GATTAAGTGG GCCCCTATCA
    GGACCTGGGA CAACTTTATA CTTAAATGTA ACCGATTTGA CAAGCTATCA GGTTTCATGG
    GACTCGTTCT GTATAAGATG GACTCCTCAC CGATCAGCCA CTTCCTACAG ACTGAAACTG
    AATCCAGTTG ATGGATCTCG AGGTCAAGAA ATAACAGTAA CAGGATCAGA ATCATCACAC
    TGTTTTAATG GACTGAACCA AGATACACAG TACACAGCTA CTGTTTTTGT GCAAACACCT
    AACCTTGAAG GACGAGGAGT TTCAATAGAT GAGCGTACAT TGATCAAGCC TACAGAATCA
    CCAACGACAC CACCTGCTCC TCCTCCACCC CCTACAATTC CACCTGCTCG GGAGGTGTGC
    AAAGGGGCCA AAGCAGACAT AGTATTCCTC ATTGACAGTT CGTGGAGTAT TGGTGATGAT
    AACTACAATA AAGTCCGTCA GTTTATCTTC AATACTATTG GAGCATTCGA TGTAATCAAC
    CCTTCTGGAA TCCAGGTCTC TTTTGTGCAG TTCAGCGATG ATGCCCAGAC GGAGTTCAAG
    TTGAACACTT ATGCCGACAA ATCACAGGCT CTAGGAGCAC TTGCCAATAT CAGATACAAA
    GGAGGAAACA CAAAAACAGG AAAGGCTCTT AAATTTGTCC ACGAGAAAGT GATGACAGTT
    GAGAATGGAA TGAGAAGATC TGTTCCTAGG GTTCTGGTAG TGGTGACTGA TGGCAGATCT
    CAGGATGATG TTAAGAAATC AGCCTTGGAC ATTCAGCAAT CTGGTTTTAG TGTCTTTGTC
    ATTGGTGTGG CTGATGTGGA TTATAATGAG CTAAGAAACA TTGGCAGTAA ACCAAGCGAG
    CGCCATGTTT TTATAGTCGA TGACTTTGAT GCTTTTGAGA AGATTGAAGA TAACCTGATC
    AACTTTGTAT GTGAGACTGC AACATCCACT TGCCCACTTA TTTACCTGGA AGGATACACT
    TCACCAGGTT TCAGAATGCT GGAAGCATAT AACCTGACAG AGAAAACTTT TGCCTCAGTA
    CAAGGTGTCT CAATGCAACC AGGATCTTTC AACAGTTTCC CTGCATACAA CCTACATAAA
    GATGCCTTCA TTTCCCTGCC TACCTCAGAA TTGCACCCAG AAGGATTACC ACCTTCATAT
    ACGATTATAT TGCTATTCCG TCTGCTGCCA GAAACTCCCA ATGAGCCATT TTCTCTATGG
    CAAATAACAG ATAAAGGTTA CAAGCCACAA GTAGGAGTAA ATCTTGATCC ATCTAAGAAA
    ACACTGTCGT TCTTTAATAA GGGAGCTGCT GGGGATACTC AGACAGTAAC ATTTGATGGG
    AATGAAGTGA AGAAACTCTT TTATGGAAGC TTCCACAAGG TTCTCATTGT GGTTTCTCCA
    TCATACGTCA GAATTTATAT TGATTGTAGT GAAGTTGCTG AAAAAGAAAT CAAAGAAGCT
    GGCAATATTA CAGCAGAAGG ATATGAAGTA CTTGGGAAGA CATTAAAGGG TGATAAAAAA
    TCTGCACCCT TCCTGCTCCA GATGTTTGAC ATTGTGTGTA CTGTGACCTG GACAAGTCGA
    GATAGATGCT GTGATATTCC TGCTACAAGG GATGAAGCCA AATGTCCAGC TCTGCCCCAT
    TCCTGTACTT GCGCACAAGA CAGCGTTGGA GCACCAGGAC CCCCAGGCCC ATCCGGTATT
    CCCGGTAGTA AAGGACCACG GGGTGACCGA GGTTTGGATG GACCAGTTGG CCCACCAGGT
    CAGCGAGGAG AACAAGGTTT GCCTGGTCCA CAAGGACCAC CTGGACCTCA AGGACCACCT
    GGACTTTCCA TTCCTGGAGA ACCTGGTCGT CAAGGAATAA AAGGAGATGC CGGTGACCCA
    GGATTGCCAG GAAGAATGGG TTCCCCAGGG GTGACAGGAC CACCAGGGCC TATGGGTCCA
    CCAGGCACAA GGGGTTTCAC AGGAAAGGAT GGTGCAGTAG GACCCAGAGG ACCACCAGGA
    CCAATGGGAG CACCTGGACC TCAGGGAGTG CCAGGTCCAA GTGGGAAACC AGGAAAGCAA
    GGAGATAATG GATTGCCAGG TCCTCTTGGA CAGAAAGGAG ACAAGGGTGA GAGGGGAGAC
    ATGGCATCTC AGAATATGAT GAGATCAGTA GCAAGACAAG TCTGTGAACA GATGATAAAT
    AGTCAAATGA GCAGATTTAA TCAGATGTTG AACCAAATTC CAAATGATTA CTATTCCAAT
    CGCAATCAGC CTGGTCCTCC AGGTCCTCCC GGTCCTCCAG GCAACGCTGG CATGAGAGGA
    GAACCTGGAG CTGCTGGCAG ATCTGGATTC CCAGGAACAC CTGGTCCTCA AGGACCACCT
    GGAGAAAGAG GAATACCAGG AGAGAAAGGG GAGAGAGGGA ACCCAGGAAT TGGATCCCAA
    GGACCCAGGG GACTTCCAGG ACCTCCAGGA CCTCCAGGCG AATCCAGAAC AGGACCTCCA
    GGATCCTCAG GCTCCACAGG ACCTCCAGGA CCTTCAGGTC GCCCCGGTAA CCAAGGAATT
    AGAGGGCCTC CTGGTCCAGC TGGATACTGT GATGCTTCAA TGTGTGCAAG CATTCCTTAC
    AATGGGCAAG GATACCCAGA ACCTTATCAG CCTGAAAGTG GACCGCAGGA GCCAGAGAAT
    CAGCCTTACG TAGTACCTAT GCAGCAAGAG AGTCCAGATG AGGATTTTGA GGACTATGGA
    CATGAATATG CAGAACATAT GCGATGGAGG AGATCCTTAC CAAAGAAATT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10388
    Clone ID Related Accession (Same CDS sequence) XM_002936200.2
    Accession Version XM_002936200.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9342bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XII) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 18, 2013 this sequence version replaced XM_002936200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGTTGGTCC ACCTACAGAC TTAAATTTTG AAATAGTAAA TGAAAATACA
    GTTCGAATGT CATGGCAAAG GCCGGCAGAA AGAATAAAGG GTTACAAGAT TACTATCGTC
    CCTACAATTG ATGGACCAGA AAAGGAACTA GATCTTCCAG CTTCTGCTAC GCAAACCATA
    CTAACTGAAC TTGTACCAGA TGTGGAATAT GTTGTGACAG CTATTTCATA TGATGAATTA
    GAGGAGAGTG TACCTGTGTT TGGCCAACTT ACAATTCAAA CAGGAGGTCG AGTGACACTT
    GAAGAGCCTA AACTTGAGAC AAGTAAATCA CCACGCTGCT CTATTAGTTC TGTTGCTGAT
    GTAGTTTTCC TTGTGGATGG ATCATGGAGT GTTGGAAGAA ACAACTTCAA ATATATTCTT
    GATTTCATGG TTTCTCTGGT CTCAGCCTTT GATATTGGGG AAGACAAAAC CAGAGTTGGT
    GTTGTGCAGT ATAGCTCTGA CACTAGGACT GAGTTTAACC TGAACAGTTA TTACAAGAAA
    GAGGAATTAG TATCAGCCAT AAAAAGAATA CCATACAAGG GAGGCAATAC AATGACAGGT
    GATGCCCTTG ATTTCCTGAT TAAGAACTCC TTTGTCAAAA CTGCCGGCTC AAGAAAAGGA
    TTTCCAAGAA TAGCTATCAT TATAACTGAT GGTAAATCCC AAGATGAAGT GGAGATTCCA
    GCAAGAGAGC TGCGTAGCTT AGGGGTTGAA GTTTTTTCCC TTGGTATAAA AGCAGCAGAT
    GCAAAAGAAC TCAAACAAAT AGCTTCTCTG CCTTCCTTGA AACATGTTTT TAATGTTGCT
    AATTTTGATT CGATTGTGGA AGTGCAAGAT GAAATAATTA CTCAAGTTTG CTCAGGAGTT
    GAAGAGCAGC TAAGTGAGCT TGTTAGTGGA GAAGAAGCTG TTGAGCCTCC TTCAGATATG
    ACTGTTACGG AAATTTCTTC CAAATCAATG AAGATCACCT GGAGACCATC GCCAAGCCAA
    ATTACAGGCT ACAGGATTCA GCTTCTTCCA ATGTTAGCGG GAAGTAAACA ACATTCACTG
    AACCTTGGCC CTCAAACTAC AAGTATAAAT GTAAAAGATC TCTCCCCTGA TACAGAATAC
    CAAATAAATT TATATGCCAT GAAAGGACTC ACAGCAAGTG AGCCAATCTC AACACTGGAA
    AAAACCCAGG CTGTGAAAAT TAAAGTCGAA TGTTCCGGTG GTGTCGATAT AAAAGCAGAT
    GTCGTTCTGC TTGTTGATGG CTCCTACAGT ATTGGTGTAG CTAATTTTGC CAAAGTTAGA
    GCCTTCCTTG AAGTATTAGT AAAAAGCTTT GATATTTCGC CCAGCAAAGT ACAGATCAGT
    CTTGTGCAAT ACAGTAGAGA TCCATTTACA GAGTTTACCT TAAACAGGTA TGACTCAATT
    GAAGACATTC TCAAAGCTGT AAATACCTTT CCATATAGAG GTGGTTCTAC CAACACTGGC
    AAAGCAATGA CCTACGTGAG AGAAAAGGTC TTTGTTGAAA TTAAAGGAGC AAGACCAAAC
    GTCCCTAGAG TCATGATTCT TATCACCGAT GGAAAGTCAT CAGATGCCTT TAAAGATCCA
    GCTATAAAAC TGCGGAATTC AGATGTTGAG ATTTTTGCAG TTGGTGTGAA GGATGCAGTG
    AGATCTGAGC TGGAGGCCAT TGCTTCTCCT CCTTCAGACA CCCATGTGTA CACAGTGGAA
    GATTTTGATG CTTTTCAAAG GATTTCTTTT GAGCTAACAC AGTCTATCTG CCTTAGAATT
    GAGCAAGAGC TACAAACAAT AAAACGTAAA TCTTTGACCC CTGCAAGAGA TTTGGCTTTC
    TCTGAGGTTA CATCCAACAG TTTTCGTGTG AACTGGTCTC CTGCGGCTGA GGATGTTTTG
    TCATACCTTG TTAAATATAA GGTGGCTGTT GGAGAAGGTG AAGAATTCAT CGTTTCCGTG
    CCAGCACCAA AAACAACCAC AGTTCTTTCA AATTTACTTC CAGAAACTAC TTATGCTGTG
    GCTGTCCTGG CAGAATACGA GGAAGGAGAG AGTCCTCCAT TAAATGGGGA AGAAACAACT
    TTAGAAGTTA CTGGCCCACC AAGAAACCTG AGAGTAAGTG ACGAAACAAC TGATAGCTTT
    AAAGTTGGCT GGTCACCAGC TCCTGGCAAT GTACTGAGGT ACAGAATTGC CTACCGGCCT
    GTGGCAGGAG GGGCAAGAAG AGAGGTTACA GTCCCAGGAA GGGAGACCGT GACAACACTC
    CAGAACCTCA CACCTGATAC AAAGTACCAA GTATCTGTGA TTCCTGAATA TCATTCGGGT
    CCTGGAAAAC CTTTGACAGG AAATGGAGCC ACAGAAGAAG TGTTGGGAGA ACCAAGGAAT
    CTGAGGGTTT CTGATGCCAC AACCTCCAGT TTAAAACTGT CCTGGGATTC GGCACCAGGC
    AAAGTGTTAC AGTATCTTGT AACTTACAAC CCTTCTGCAG AAGGGGAAAC TAAAGAGGTT
    ACAGTAAGAG GAAATACAAC CAGTACAGTA CTGAAGGGAT TAGACCCAAG CACCACATAC
    TCCCTTTCTG TAGCTGCACT CTATAGATCT GGCGCTGGCT CATTACTTAC TGGGGAAGGA
    ACAACTCTTG ATGAACGTGG TTCTCCTCGT AACCTGGTAA CCAAAGACAT TACAGACACT
    ACAATTGGAG CATCATGGAC AGCGGCCCCA GGGATGGTTC GTGGTTACAG AATTGCATGG
    AAGTCTCTTT TTGATGATGA CTCTGGAGAA AAGAATGTTC CTGGTGGTAT TACTAACACT
    GTGCTGGAAA ACCTGAGGCC AGAAACAAAG TATAAAATTT CAGTGTTTGC TAATTATAGA
    AGTGGAGAAG GAGACCCTCT TGAAGGAGAA GCCACCACTG AAGCGTCACC AAGTGCCAAG
    CTGCTTAAAG TCAGTGATGA GACAGAAACT ACAATGAAGG TAACCTGGCA GCCTGCCCCA
    GGAAATGTTG CAAATTATCG GGTTATATAT CGGCCGAAGC TTGGAGGAAG GCAAGTGGTC
    GCTAAAGTGC CAGCTACAGT CTCCTCAACA GTACTGAAAA GGCTTCAACC TCAAACAACC
    TATGATATTA CAGTTGTTCC TGTTTATAAG GCAGGGGATG GAAAACCAAG ACAAGGAGAA
    GGGACAACAG CTTCTCCTTT CAAGCCCCCA AGGAATCTGA AAACATCTGA CCCCACAATC
    TCAACTTTCC GAGTCACCTG GGAACCTGCT CCTGGTGAAG TTAGGGGTTA TAAAATCACA
    TATTTTCCTT TTGGAGAGGA GCAATATTTG GGAGAGTTAG TAGTTGGACC ATATGATAAC
    ACTGTGGTAC TAGAAGAACT CAGGGCAGGA ACATCTTATA AAGTCAATGT ATTTGGAGTT
    TTTGAAAAAG GAGAGAGTGT ACCTTTAATT GGACAAGAAA TGACAACATT GTCAGATGCT
    CCTGAAGTTC GCATTGATTC GTCAGGCCTG GAATGCAAAA CTAAAGCTGC AGCTGATATA
    GTGTTGCTGG TGGATGGCTC TTGGAGTATC GGCCGCCCAA ATTTTAGGAC CGTCAGGAGT
    TTTATAGCAC GACTGGTTGA AGTCTTTGAT ATTGGCTCAG ACAGAGTGCA GATTGCCTTG
    GCCCAATACA GTGGGGACCC TCGATCTGAA TGGCAACTCA ATGCTCATAG CACAAAGAAG
    TCTTTAATGG ATGCCGTTGC AAACCTTCCT TACAAGGGGG GTAACACTCT TACAGGCATG
    GCTCTGAATT TCATCTTGCA AAATAACTTC AAGGCAGAGG CAGGCATGAG AGCAAAGTCA
    AAGAAAATTG GTGTCTTAAT CACTGATGGA AAGTCACAAG ATGACATTGT TGCACCTTCC
    AAAAAGTTAC GAGACCAGGG AATAGACTTA TTTGCAATTG GTATCAAAAA TGCTGATGAA
    AATGAACTGA GGCAGATTGG CTCAGATCCA GATGAGACTT ACGTATTTAA CGTTGCCGAT
    TTTTCTTTGC TTGTCAATAT TGTTGATGAC CTCACTACCA ATCTATGTAA CAGTGTGAAA
    GGCTCAGGAA ATGAACTGGA GCCACCTTCA GACCTAGTAA CATCAGAGCC TACACCACGG
    AGCTTCAGAG TCACATGGAA TCCACCTTCT CACAGTGTAG ACCGATTTAG GGTGGAATAT
    TATCCAGTGT CTGGTGGTCG ACCTCTGGAG GTATTTGTTG GCAGATCAGA TAGAACTACG
    GTTTTGACTA ATTTAAAGCC AGAAACAGAG TATGTTGTGA AAGTGTATTC TGTGTTAGAA
    GGAGAAAGCA GTGAACCATT AGAAGGCACA GAAACGACAT TGCCCATCCC TTCAGTCAGA
    AATCTTAACA TATATGATAT AGGAACCAGA ACAATGAGAG TTCGATGGGA AGCTGCGCTT
    GGAGCTACTG GTTATTTATT GCAGTATGAG CCAGTAAACG CTACTGTGCC TTCAACTGAA
    AAAGAGTTGA GAGTTGGTGC ATCGGTGACA GATGTACTTC TGGAAGATTT ATTCCCCAAC
    ACTGAGTACA CCTTGACTGT ATATGCACTT TTTGATGAGC TGATCAGTGA TCCTCTTACC
    TCACAGGAAG TCACTTTACC CATTCAAGGC GTGGGGAACA TTAGAGTGAG AGATATCACT
    CACAGCTCAA TGAATGTCCT TTGGAGTGCT GCTCCTGGAA ATGTTCTCAA ATATATAGTA
    AGATATAAAA CAGAGGAAGA AGAAGAATTC AAAGAGGTGG AAGTGGATGG ATCAAGGACA
    AGTACACGAC TTACTGACCT TTTCTCACAA ACTCTATATG AAATCAGTTT AACTGCAGTA
    TATACTACAC AAGGAACTAC AGCACCAGTA CCAGCTCCAG CAAACCTCCG GTTTTCTGAA
    ATAGCAGTAA CAAGTTTCCG AGGTACTTGG GAGCATGGGG CTCCAGATGT AGCTTTGTAC
    AGGATAACGT GGGCACCAGT TGGAGCCATA GAAAAAAAGA AAGAGACAAT TGTTGATGGT
    GATATAAACA CTTTGGTTTT TGATGAACTG GAACCAGACA CAGAGTATGA GGTTTCAGTA
    ACTGCGATTT ACCCTGACGA ATCAGAGAGT GAAGATCTGA TCGGTGTTGA AAGGACATTG
    CCTGAAATAA TTTCTACAAC ACCTGCCCTA AGGACTGGCC CTAGGAATCT ACAGGTGTTC
    AATGCAACAC AGAATAGTTT GACTGTACGA TGGGATCGTG CTATGGGCGA TGTGCAAAAA
    TACAATATCT ATTATAAGCC TGTTTCTGCT GCTGGTGCTG AACAAATGAC AAGTGTTGGT
    GGAAAGCAGA ACAGTGTTGT TCTTCAGCGA CTGCAGCCAG ACACTCCATA CAACATTGTT
    GTTTCATCAA GGTACATTGG AGATGAGGAA GGAGGAAATA TCTCAGGGAG AGGAAAAACA
    AAGCCCTTAA ATACAGTGAA GAATCTAAGA GTGTATGACC CAACAACAAG CACTTTAAAT
    GTACGTTGGG AAGCTGCAGA AGGAAATGTG CGGCGGTATA AAATTTACTA TGTACCAACT
    GCTGGAGGAC CAGAGGAAAC GGTGACAGTG CCCGGAAATA CAATCTATTC AGTTCTTAGG
    AATCTTCAGT CTGATACATC ATATACAGTA ACTGTTGTTC CTGTCTATTC TGAAGGAGAA
    GGAGGTCGTA TGTCAGAAAC AGGAAAGACT TTGGTGCGGG GAACAGTTCG AAATATTCAA
    GTATACAACC CCACAACAAA CACTTTGAAT GTTCGCTGGG AGCCTGCGCC TGGACAGGTC
    CAGCAATATA AAGTCAACTA TGCTCCACTT CTTGGAACAA GGCCTACTGA GTCTGTTGTA
    GTTCCTGGCA ATACTCACAA CGCGCTGCTG GAGCAACTTA TTCCTGACAC ACAGTACTCT
    GTAAATGTTG TGGCTCTTTA TGCAGATGGT GAGGGGGATC CTAGCCCTGG TGAAGGAAAA
    ACATTACCTC GCAGTGGGCC AAAAAATATT AGAGTTTTTG GAGAGACCAC TAACAGTCTC
    TCTGTGAACT GGGATCATGC TGATGGACCA GTCCAACAAT ACAGAATCAT CTATTCACCA
    ACTGTTGGAG ACCCCATTGA TGAATATACA ACAGTGCCTG GTAGGAGAAA TAATGTGGTA
    CTGCAGCCCC TTTTGTCTGA CACGCCATAT AAAATTACTG TGGTGGCAGT TTATGCTGAT
    GGTGATGGAG GACAACTGAC AGGAAAAGGA AGGACAGTGG GTTTGATTGC ACCACGGAAC
    CTTCAAGTGT CTGATGAATG GTACACAAGA TTTAGGGTTT CCTGGGATAG AATACCATCT
    CCTGTTCTTG GATACAAGCT GGTTTATCAG CCACGTGATT CAGATGAATC AATGGAAGTA
    TTTGTCGGGG ATGTGAGCTC CTACACTTTG CAGAACCTGT CTCCTGGCAC CACATATGAT
    GTGAAAGTTT ACGCTCAGTA TGATACAGGA TTAAGTGGGC CCCTATCAGG ACCTGGGACA
    ACTTTATACT TAAATGTAAC CGATTTGACA AGCTATCAGG TTTCATGGGA CTCGTTCTGT
    ATAAGATGGA CTCCTCACCG ATCAGCCACT TCCTACAGAC TGAAACTGAA TCCAGTTGAT
    GGATCTCGAG GTCAAGAAAT AACAGTAACA GGATCAGAAT CATCACACTG TTTTAATGGA
    CTGAACCAAG ATACACAGTA CACAGCTACT GTTTTTGTGC AAACACCTAA CCTTGAAGGA
    CGAGGGGTTT CAATAGATGA GCGTACATTG ATCAAGCCTA CAGAATCACC AACGACACCA
    CCTGCTCCTC CTCCACCCCC TACAATTCCA CCTGCTCGGG AGGTGTGCAA AGGGGCCAAA
    GCAGACATAG TATTCCTCAT TGACAGTTCG TGGAGTATTG GTGATGATAA CTACAATAAA
    GTCCGTCAGT TTATCTTCAA TACTATTGGA GCATTCGATG TAATCAACCC TTCTGGAATC
    CAGGTCTCTT TTGTGCAGTT CAGCGATGAT GCCCAGACTG AGTTCAAGTT GAACACTTAT
    GCCGACAAAT CACAGGCTCT AGGAGCACTT GCCAATATCA GATACAAAGG AGGAAACACA
    AAAACAGGAA AGGCTCTTAA ATTTGTCCAC GAGAAAGTGA TGACAGTTGA GAATGGAATG
    AGAAGATCTG TTCCTAGGGT TCTGGTAGTG GTGACTGATG GCAGATCTCA GGATGATGTT
    AAGAAATCAG CCTTGGACAT TCAGCAATCT GGTTTTAGTG TCTTTGTCAT TGGTGTGGCT
    GATGTGGATT ATAATGAGCT AAGAAACATT GGCAGTAAAC CAAGCGAGCG CCATGTTTTT
    ATAGTCGATG ACTTTGATGC TTTTGAGAAG ATTGAAGATA ACCTGATCAA CTTTGTATGT
    GAGACTGCAA CATCCACTTG CCCACTTATT TACCTGGAAG GATACACTTC ACCAGGTTTC
    AGAATGCTGG AAGCATATAA CCTGACAGAG AAAACTTTTG CCTCAGTACA AGGTGTCTCA
    ATGCAACCAG GATCTTTCAA CAGTTTCCCT GCATACAACC TACATAAAGA TGCCTTCATT
    TCCCTGCCTA CCTCAGAATT GCACCCAGAA GGATTACCGC CTTCATATAC GATTATATTG
    CTATTCCGTC TGCTGCCAGA AACTCCCAAT GAGCCATTTT CTCTATGGCA AATAACAGAT
    AAAGGTTACA AGCCACAAGT AGGAGTAAAT CTTGATCCAT CTAAGAAAAC ACTGTCGTTC
    TTTAATAAGG GAGCTGCTGG GGATACTCAG ACAGTAACAT TTGATGGGAA TGAAGTGAAG
    AAACTCTTTT ATGGAAGCTT CCACAAGGTT CTCATTGTGG TTTCTCCATC ATATGTCAGA
    ATTTATATTG ATTGTAGTGA AGTTGCTGAA AAAGAAATCA AAGAAGCTGG CAATATTACA
    GCAGAAGGAT ATGAAGTACT TGGGAAGACA TTAAAGGGTG ATAAAAAATC TGCACCCTTC
    CTGCTCCAGA TGTTTGACAT TGTGTGTACT GTGACCTGGA CAAGTCGAGA TAGATGCTGT
    GATATTCCTG CTACAAGGGA TGAAGCCAAA TGTCCAGCTC TGCCCCATTC CTGTACTTGC
    GCACAAGACA GCGTTGGAGC ACCAGGACCC CCAGGCCCAT CCGGTATTCC CGGTAGTAAA
    GGACCACGGG GTGACCGAGG TTTGGATGGA CCAGTTGGCC CACCAGGTCA GCGAGGAGAA
    CAAGGTTTGC CTGGTCCACA AGGACCACCT GGACCTCAAG GACCACCTGG ACTTTCCATT
    CCTGGAGAAC CTGGTCGTCA AGGAATAAAA GGAGATGCCG GTGACCCAGG ATTGCCAGGA
    AGAATGGGTT CCCCAGGGGT GACAGGACCA CCAGGGCCTA TGGGTCCACC AGGCACAAGG
    GGTTTCACAG GAAAGGATGG TGCAGTAGGA CCCAGAGGAC CACCAGGACC AATGGGAGCA
    CCTGGACCTC AGGGAGTGCC AGGTCCAAGT GGGAAACCAG GAAAGCAAGG AGATAATGGA
    TTGCCAGGTC CTCTTGGACA GAAAGGAGAC AAGGGTGAGA GGGGAGACAT GGCATCTCAG
    AATATGATGA GATCAGTAGC AAGACAAGTC TGTGAACAGA TGATAAATAG TCAAATGAGC
    AGATTTAATC AGATGTTGAA CCAAATTCCA AATGATTACT ATTCCAATCG CAATCAGCCT
    GGTCCTCCAG GTCCTCCCGG TCCTCCAGGC AACGCTGGCA TGAGAGGAGA ACCTGGAGCT
    GCTGGCAGAT CTGGATTCCC AGGAACACCT GGTCCTCAAG GACCACCTGG AGAAAGAGGA
    ATACCAGGAG AGAAAGGGGA GAGAGGGAAC CCAGGAATTG GATCCCAAGG ACCCAGGGGA
    CTTCCAGGAC CTCCAGGACC TCCAGGCGAA TCCAGAACAG GACCTCCAGG ATCCTCAGGC
    TCCACAGGAC CTCCAGGACC TTCAGGTCGC CCCGGTAACC AAGGAATTAG AGGGCCTCCT
    GGTCCAGCTG GATACTGTGA TGCTTCAATG TGTGCAAGCA TTCCTTACAA TGGGCAAGGA
    TACCCAGAAC CTTATCAGCC TGAAAGTGGA CCGCAGGAGC CAGAGAATCA GCCTTACGTA
    GTACCTATGC AGCAAGAGAG TCCAGATGAG GATTTTGAGG ACTATGGACA TGAATATGCA
    GAACATATGC GATGGAGGAG ATCCTTACCA AAGAAATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002936246.2
    CDS171..9512
    Translation

    Target ORF information:

    RefSeq Version XM_002936200.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen type XII alpha 1 chain (col12a1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002936200.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGTTGGTCC ACCTACAGAC TTAAATTTTG AAATAGTAAA TGAAAATACA
    GTTCGAATGT CATGGCAAAG GCCGGCAGAA AGAATAAAGG GTTACAAGAT TACTATCGTC
    CCTACAATTG ATGGACCAGA AAAGGAACTA GATCTTCCAG CTTCTGCTAC GCAAACCATA
    CTAACTGAAC TTGTACCAGA TGTGGAATAT GTTGTGACAG CTATTTCATA TGATGAATTA
    GAGGAGAGTG TACCTGTGTT TGGCCAACTT ACAATTCAAA CAGGAGGTCG AGTGACACTT
    GAAGAGCCTA AACTTGAGAC AAGTAAATCA CCACGCTGCT CTATTAGTTC TGTTGCTGAT
    GTAGTTTTCC TTGTGGATGG ATCATGGAGT GTTGGAAGAA ACAACTTCAA ATATATTCTT
    GATTTCATGG TTTCTCTGGT CTCAGCCTTT GATATTGGGG AAGACAAAAC CAGAGTTGGT
    GTTGTGCAGT ATAGCTCTGA CACTAGGACT GAGTTTAACC TGAACAGTTA TTACAAGAAA
    GAGGAATTAG TATCAGCCAT AAAAAGAATA CCATACAAGG GAGGCAATAC AATGACAGGT
    GATGCCCTTG ATTTCCTGAT TAAGAACTCC TTTGTCAAAA CTGCCGGCTC AAGAAAAGGA
    TTTCCAAGAA TAGCTATCAT TATAACTGAT GGTAAATCCC AAGATGAAGT GGAGATTCCA
    GCAAGAGAGC TGCGTAGCTT AGGGGTTGAA GTTTTTTCCC TTGGTATAAA AGCAGCAGAT
    GCAAAAGAAC TCAAACAAAT AGCTTCTCTG CCTTCCTTGA AACATGTTTT TAATGTTGCT
    AATTTTGATT CGATTGTGGA AGTGCAAGAT GAAATAATTA CTCAAGTTTG CTCAGGAGTT
    GAAGAGCAGC TAAGTGAGCT TGTTAGTGGA GAAGAAGCTG TTGAGCCTCC TTCAGATATG
    ACTGTTACGG AAATTTCTTC CAAATCAATG AAGATCACCT GGAGACCATC GCCAAGCCAA
    ATTACAGGCT ACAGGATTCA GCTTCTTCCA ATGTTAGCGG GAAGTAAACA ACATTCACTG
    AACCTTGGCC CTCAAACTAC AAGTATAAAT GTAAAAGATC TCTCCCCTGA TACAGAATAC
    CAAATAAATT TATATGCCAT GAAAGGACTC ACAGCAAGTG AGCCAATCTC AACACTGGAA
    AAAACCCAGG CTGTGAAAAT TAAAGTCGAA TGTTCCGGTG GTGTCGATAT AAAAGCAGAT
    GTCGTTCTGC TTGTTGATGG CTCCTACAGT ATTGGTGTAG CTAATTTTGC CAAAGTTAGA
    GCCTTCCTTG AAGTATTAGT AAAAAGCTTT GATATTTCGC CCAGCAAAGT ACAGATCAGT
    CTTGTGCAAT ACAGTAGAGA TCCATTTACA GAGTTTACCT TAAACAGGTA TGACTCAATT
    GAAGACATTC TCAAAGCTGT AAATACCTTT CCATATAGAG GTGGTTCTAC CAACACTGGC
    AAAGCAATGA CCTACGTGAG AGAAAAGGTC TTTGTTGAAA TTAAAGGAGC AAGACCAAAC
    GTCCCTAGAG TCATGATTCT TATCACCGAT GGAAAGTCAT CAGATGCCTT TAAAGATCCA
    GCTATAAAAC TGCGGAATTC AGATGTTGAG ATTTTTGCAG TTGGTGTGAA GGATGCAGTG
    AGATCTGAGC TGGAGGCCAT TGCTTCTCCT CCTTCAGACA CCCATGTGTA CACAGTGGAA
    GATTTTGATG CTTTTCAAAG GATTTCTTTT GAGCTAACAC AGTCTATCTG CCTTAGAATT
    GAGCAAGAGC TACAAACAAT AAAACGTAAA TCTTTGACCC CTGCAAGAGA TTTGGCTTTC
    TCTGAGGTTA CATCCAACAG TTTTCGTGTG AACTGGTCTC CTGCGGCTGA GGATGTTTTG
    TCATACCTTG TTAAATATAA GGTGGCTGTT GGAGAAGGTG AAGAATTCAT CGTTTCCGTG
    CCAGCACCAA AAACAACCAC AGTTCTTTCA AATTTACTTC CAGAAACTAC TTATGCTGTG
    GCTGTCCTGG CAGAATACGA GGAAGGAGAG AGTCCTCCAT TAAATGGGGA AGAAACAACT
    TTAGAAGTTA CTGGCCCACC AAGAAACCTG AGAGTAAGTG ACGAAACAAC TGATAGCTTT
    AAAGTTGGCT GGTCACCAGC TCCTGGCAAT GTACTGAGGT ACAGAATTGC CTACCGGCCT
    GTGGCAGGAG GGGCAAGAAG AGAGGTTACA GTCCCAGGAA GGGAGACCGT GACAACACTC
    CAGAACCTCA CACCTGATAC AAAGTACCAA GTATCTGTGA TTCCTGAATA TCATTCGGGT
    CCTGGAAAAC CTTTGACAGG AAATGGAGCC ACAGAAGAAG TGTTGGGAGA ACCAAGGAAT
    CTGAGGGTTT CTGATGCCAC AACCTCCAGT TTAAAACTGT CCTGGGATTC GGCACCAGGC
    AAAGTGTTAC AGTATCTTGT AACTTACAAC CCTTCTGCAG AAGGGGAAAC TAAAGAGGTT
    ACAGTAAGAG GAAATACAAC CAGTACAGTA CTGAAGGGAT TAGACCCAAG CACCACATAC
    TCCCTTTCTG TAGCTGCACT CTATAGATCT GGCGCTGGCT CATTACTTAC TGGGGAAGGA
    ACAACTCTTG ATGAACGTGG TTCTCCTCGT AACCTGGTAA CCAAAGACAT TACAGACACT
    ACAATTGGAG CATCATGGAC AGCGGCCCCA GGGATGGTTC GTGGTTACAG AATTGCATGG
    AAGTCTCTTT TTGATGATGA CTCTGGAGAA AAGAATGTTC CTGGTGGTAT TACTAACACT
    GTGCTGGAAA ACCTGAGGCC AGAAACAAAG TATAAAATTT CAGTGTTTGC TAATTATAGA
    AGTGGAGAAG GAGACCCTCT TGAAGGAGAA GCCACCACTG AAGCGTCACC AAGTGCCAAG
    CTGCTTAAAG TCAGTGATGA GACAGAAACT ACAATGAAGG TAACCTGGCA GCCTGCCCCA
    GGAAATGTTG CAAATTATCG GGTTATATAT CGGCCGAAGC TTGGAGGAAG GCAAGTGGTC
    GCTAAAGTGC CAGCTACAGT CTCCTCAACA GTACTGAAAA GGCTTCAACC TCAAACAACC
    TATGATATTA CAGTTGTTCC TGTTTATAAG GCAGGGGATG GAAAACCAAG ACAAGGAGAA
    GGGACAACAG CTTCTCCTTT CAAGCCCCCA AGGAATCTGA AAACATCTGA CCCCACAATC
    TCAACTTTCC GAGTCACCTG GGAACCTGCT CCTGGTGAAG TTAGGGGTTA TAAAATCACA
    TATTTTCCTT TTGGAGAGGA GCAATATTTG GGAGAGTTAG TAGTTGGACC ATATGATAAC
    ACTGTGGTAC TAGAAGAACT CAGGGCAGGA ACATCTTATA AAGTCAATGT ATTTGGAGTT
    TTTGAAAAAG GAGAGAGTGT ACCTTTAATT GGACAAGAAA TGACAACATT GTCAGATGCT
    CCTGAAGTTC GCATTGATTC GTCAGGCCTG GAATGCAAAA CTAAAGCTGC AGCTGATATA
    GTGTTGCTGG TGGATGGCTC TTGGAGTATC GGCCGCCCAA ATTTTAGGAC CGTCAGGAGT
    TTTATAGCAC GACTGGTTGA AGTCTTTGAT ATTGGCTCAG ACAGAGTGCA GATTGCCTTG
    GCCCAATACA GTGGGGACCC TCGATCTGAA TGGCAACTCA ATGCTCATAG CACAAAGAAG
    TCTTTAATGG ATGCCGTTGC AAACCTTCCT TACAAGGGGG GTAACACTCT TACAGGCATG
    GCTCTGAATT TCATCTTGCA AAATAACTTC AAGGCAGAGG CAGGCATGAG AGCAAAGTCA
    AAGAAAATTG GTGTCTTAAT CACTGATGGA AAGTCACAAG ATGACATTGT TGCACCTTCC
    AAAAAGTTAC GAGACCAGGG AATAGACTTA TTTGCAATTG GTATCAAAAA TGCTGATGAA
    AATGAACTGA GGCAGATTGG CTCAGATCCA GATGAGACTT ACGTATTTAA CGTTGCCGAT
    TTTTCTTTGC TTGTCAATAT TGTTGATGAC CTCACTACCA ATCTATGTAA CAGTGTGAAA
    GGCTCAGGAA ATGAACTGGA GCCACCTTCA GACCTAGTAA CATCAGAGCC TACACCACGG
    AGCTTCAGAG TCACATGGAA TCCACCTTCT CACAGTGTAG ACCGATTTAG GGTGGAATAT
    TATCCAGTGT CTGGTGGTCG ACCTCTGGAG GTATTTGTTG GCAGATCAGA TAGAACTACG
    GTTTTGACTA ATTTAAAGCC AGAAACAGAG TATGTTGTGA AAGTGTATTC TGTGTTAGAA
    GGAGAAAGCA GTGAACCATT AGAAGGCACA GAAACGACAT TGCCCATCCC TTCAGTCAGA
    AATCTTAACA TATATGATAT AGGAACCAGA ACAATGAGAG TTCGATGGGA AGCTGCGCTT
    GGAGCTACTG GTTATTTATT GCAGTATGAG CCAGTAAACG CTACTGTGCC TTCAACTGAA
    AAAGAGTTGA GAGTTGGTGC ATCGGTGACA GATGTACTTC TGGAAGATTT ATTCCCCAAC
    ACTGAGTACA CCTTGACTGT ATATGCACTT TTTGATGAGC TGATCAGTGA TCCTCTTACC
    TCACAGGAAG TCACTTTACC CATTCAAGGC GTGGGGAACA TTAGAGTGAG AGATATCACT
    CACAGCTCAA TGAATGTCCT TTGGAGTGCT GCTCCTGGAA ATGTTCTCAA ATATATAGTA
    AGATATAAAA CAGAGGAAGA AGAAGAATTC AAAGAGGTGG AAGTGGATGG ATCAAGGACA
    AGTACACGAC TTACTGACCT TTTCTCACAA ACTCTATATG AAATCAGTTT AACTGCAGTA
    TATACTACAC AAGGAACTAC AGCACCAGTA CCAGCTCCAG CAAACCTCCG GTTTTCTGAA
    ATAGCAGTAA CAAGTTTCCG AGGTACTTGG GAGCATGGGG CTCCAGATGT AGCTTTGTAC
    AGGATAACGT GGGCACCAGT TGGAGCCATA GAAAAAAAGA AAGAGACAAT TGTTGATGGT
    GATATAAACA CTTTGGTTTT TGATGAACTG GAACCAGACA CAGAGTATGA GGTTTCAGTA
    ACTGCGATTT ACCCTGACGA ATCAGAGAGT GAAGATCTGA TCGGTGTTGA AAGGACATTG
    CCTGAAATAA TTTCTACAAC ACCTGCCCTA AGGACTGGCC CTAGGAATCT ACAGGTGTTC
    AATGCAACAC AGAATAGTTT GACTGTACGA TGGGATCGTG CTATGGGCGA TGTGCAAAAA
    TACAATATCT ATTATAAGCC TGTTTCTGCT GCTGGTGCTG AACAAATGAC AAGTGTTGGT
    GGAAAGCAGA ACAGTGTTGT TCTTCAGCGA CTGCAGCCAG ACACTCCATA CAACATTGTT
    GTTTCATCAA GGTACATTGG AGATGAGGAA GGAGGAAATA TCTCAGGGAG AGGAAAAACA
    AAGCCCTTAA ATACAGTGAA GAATCTAAGA GTGTATGACC CAACAACAAG CACTTTAAAT
    GTACGTTGGG AAGCTGCAGA AGGAAATGTG CGGCGGTATA AAATTTACTA TGTACCAACT
    GCTGGAGGAC CAGAGGAAAC GGTGACAGTG CCCGGAAATA CAATCTATTC AGTTCTTAGG
    AATCTTCAGT CTGATACATC ATATACAGTA ACTGTTGTTC CTGTCTATTC TGAAGGAGAA
    GGAGGTCGTA TGTCAGAAAC AGGAAAGACT TTGGTGCGGG GAACAGTTCG AAATATTCAA
    GTATACAACC CCACAACAAA CACTTTGAAT GTTCGCTGGG AGCCTGCGCC TGGACAGGTC
    CAGCAATATA AAGTCAACTA TGCTCCACTT CTTGGAACAA GGCCTACTGA GTCTGTTGTA
    GTTCCTGGCA ATACTCACAA CGCGCTGCTG GAGCAACTTA TTCCTGACAC ACAGTACTCT
    GTAAATGTTG TGGCTCTTTA TGCAGATGGT GAGGGGGATC CTAGCCCTGG TGAAGGAAAA
    ACATTACCTC GCAGTGGGCC AAAAAATATT AGAGTTTTTG GAGAGACCAC TAACAGTCTC
    TCTGTGAACT GGGATCATGC TGATGGACCA GTCCAACAAT ACAGAATCAT CTATTCACCA
    ACTGTTGGAG ACCCCATTGA TGAATATACA ACAGTGCCTG GTAGGAGAAA TAATGTGGTA
    CTGCAGCCCC TTTTGTCTGA CACGCCATAT AAAATTACTG TGGTGGCAGT TTATGCTGAT
    GGTGATGGAG GACAACTGAC AGGAAAAGGA AGGACAGTGG GTTTGATTGC ACCACGGAAC
    CTTCAAGTGT CTGATGAATG GTACACAAGA TTTAGGGTTT CCTGGGATAG AATACCATCT
    CCTGTTCTTG GATACAAGCT GGTTTATCAG CCACGTGATT CAGATGAATC AATGGAAGTA
    TTTGTCGGGG ATGTGAGCTC CTACACTTTG CAGAACCTGT CTCCTGGCAC CACATATGAT
    GTGAAAGTTT ACGCTCAGTA TGATACAGGA TTAAGTGGGC CCCTATCAGG ACCTGGGACA
    ACTTTATACT TAAATGTAAC CGATTTGACA AGCTATCAGG TTTCATGGGA CTCGTTCTGT
    ATAAGATGGA CTCCTCACCG ATCAGCCACT TCCTACAGAC TGAAACTGAA TCCAGTTGAT
    GGATCTCGAG GTCAAGAAAT AACAGTAACA GGATCAGAAT CATCACACTG TTTTAATGGA
    CTGAACCAAG ATACACAGTA CACAGCTACT GTTTTTGTGC AAACACCTAA CCTTGAAGGA
    CGAGGGGTTT CAATAGATGA GCGTACATTG ATCAAGCCTA CAGAATCACC AACGACACCA
    CCTGCTCCTC CTCCACCCCC TACAATTCCA CCTGCTCGGG AGGTGTGCAA AGGGGCCAAA
    GCAGACATAG TATTCCTCAT TGACAGTTCG TGGAGTATTG GTGATGATAA CTACAATAAA
    GTCCGTCAGT TTATCTTCAA TACTATTGGA GCATTCGATG TAATCAACCC TTCTGGAATC
    CAGGTCTCTT TTGTGCAGTT CAGCGATGAT GCCCAGACTG AGTTCAAGTT GAACACTTAT
    GCCGACAAAT CACAGGCTCT AGGAGCACTT GCCAATATCA GATACAAAGG AGGAAACACA
    AAAACAGGAA AGGCTCTTAA ATTTGTCCAC GAGAAAGTGA TGACAGTTGA GAATGGAATG
    AGAAGATCTG TTCCTAGGGT TCTGGTAGTG GTGACTGATG GCAGATCTCA GGATGATGTT
    AAGAAATCAG CCTTGGACAT TCAGCAATCT GGTTTTAGTG TCTTTGTCAT TGGTGTGGCT
    GATGTGGATT ATAATGAGCT AAGAAACATT GGCAGTAAAC CAAGCGAGCG CCATGTTTTT
    ATAGTCGATG ACTTTGATGC TTTTGAGAAG ATTGAAGATA ACCTGATCAA CTTTGTATGT
    GAGACTGCAA CATCCACTTG CCCACTTATT TACCTGGAAG GATACACTTC ACCAGGTTTC
    AGAATGCTGG AAGCATATAA CCTGACAGAG AAAACTTTTG CCTCAGTACA AGGTGTCTCA
    ATGCAACCAG GATCTTTCAA CAGTTTCCCT GCATACAACC TACATAAAGA TGCCTTCATT
    TCCCTGCCTA CCTCAGAATT GCACCCAGAA GGATTACCGC CTTCATATAC GATTATATTG
    CTATTCCGTC TGCTGCCAGA AACTCCCAAT GAGCCATTTT CTCTATGGCA AATAACAGAT
    AAAGGTTACA AGCCACAAGT AGGAGTAAAT CTTGATCCAT CTAAGAAAAC ACTGTCGTTC
    TTTAATAAGG GAGCTGCTGG GGATACTCAG ACAGTAACAT TTGATGGGAA TGAAGTGAAG
    AAACTCTTTT ATGGAAGCTT CCACAAGGTT CTCATTGTGG TTTCTCCATC ATATGTCAGA
    ATTTATATTG ATTGTAGTGA AGTTGCTGAA AAAGAAATCA AAGAAGCTGG CAATATTACA
    GCAGAAGGAT ATGAAGTACT TGGGAAGACA TTAAAGGGTG ATAAAAAATC TGCACCCTTC
    CTGCTCCAGA TGTTTGACAT TGTGTGTACT GTGACCTGGA CAAGTCGAGA TAGATGCTGT
    GATATTCCTG CTACAAGGGA TGAAGCCAAA TGTCCAGCTC TGCCCCATTC CTGTACTTGC
    GCACAAGACA GCGTTGGAGC ACCAGGACCC CCAGGCCCAT CCGGTATTCC CGGTAGTAAA
    GGACCACGGG GTGACCGAGG TTTGGATGGA CCAGTTGGCC CACCAGGTCA GCGAGGAGAA
    CAAGGTTTGC CTGGTCCACA AGGACCACCT GGACCTCAAG GACCACCTGG ACTTTCCATT
    CCTGGAGAAC CTGGTCGTCA AGGAATAAAA GGAGATGCCG GTGACCCAGG ATTGCCAGGA
    AGAATGGGTT CCCCAGGGGT GACAGGACCA CCAGGGCCTA TGGGTCCACC AGGCACAAGG
    GGTTTCACAG GAAAGGATGG TGCAGTAGGA CCCAGAGGAC CACCAGGACC AATGGGAGCA
    CCTGGACCTC AGGGAGTGCC AGGTCCAAGT GGGAAACCAG GAAAGCAAGG AGATAATGGA
    TTGCCAGGTC CTCTTGGACA GAAAGGAGAC AAGGGTGAGA GGGGAGACAT GGCATCTCAG
    AATATGATGA GATCAGTAGC AAGACAAGTC TGTGAACAGA TGATAAATAG TCAAATGAGC
    AGATTTAATC AGATGTTGAA CCAAATTCCA AATGATTACT ATTCCAATCG CAATCAGCCT
    GGTCCTCCAG GTCCTCCCGG TCCTCCAGGC AACGCTGGCA TGAGAGGAGA ACCTGGAGCT
    GCTGGCAGAT CTGGATTCCC AGGAACACCT GGTCCTCAAG GACCACCTGG AGAAAGAGGA
    ATACCAGGAG AGAAAGGGGA GAGAGGGAAC CCAGGAATTG GATCCCAAGG ACCCAGGGGA
    CTTCCAGGAC CTCCAGGACC TCCAGGCGAA TCCAGAACAG GACCTCCAGG ATCCTCAGGC
    TCCACAGGAC CTCCAGGACC TTCAGGTCGC CCCGGTAACC AAGGAATTAG AGGGCCTCCT
    GGTCCAGCTG GATACTGTGA TGCTTCAATG TGTGCAAGCA TTCCTTACAA TGGGCAAGGA
    TACCCAGAAC CTTATCAGCC TGAAAGTGGA CCGCAGGAGC CAGAGAATCA GCCTTACGTA
    GTACCTATGC AGCAAGAGAG TCCAGATGAG GATTTTGAGG ACTATGGACA TGAATATGCA
    GAACATATGC GATGGAGGAG ATCCTTACCA AAGAAATTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36220
    Clone ID Related Accession (Same CDS sequence) XM_018094326.1 , XM_012963500.1
    Accession Version XM_018094326.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGGCCTGGA ATGCAAAACT AAAGCTGCAG CTGATATAGT GTTGCTGGTG
    GATGGCTCTT GGAGTATCGG CCGCCCAAAT TTTAGGACCG TCAGGAGTTT TATAGCACGA
    CTGGTTGAAG TCTTTGATAT TGGCTCAGAC AGAGTGCAGA TTGCCTTGGC CCAATACAGT
    GGGGACCCTC GATCTGAATG GCAACTCAAT GCTCATAGCA CAAAGAAGTC TTTAATGGAT
    GCCGTTGCAA ACCTTCCTTA CAAGGGGGGT AACACTCTTA CAGGCATGGC TCTGAATTTC
    ATCTTGCAAA ATAACTTCAA GGCAGAGGCA GGCATGAGAG CAAAGTCAAA GAAAATTGGT
    GTCTTAATCA CTGATGGAAA GTCACAAGAT GACATTGTTG CACCTTCCAA AAAGTTACGA
    GACCAGGGAA TAGACTTATT TGCAATTGGT ATCAAAAATG CTGATGAAAA TGAACTGAGG
    CAGATTGGCT CAGATCCAGA TGAGACTTAC GTATTTAACG TTGCCGATTT TTCTTTGCTT
    GTCAATATTG TTGATGACCT CACTACCAAT CTATGTAACA GTGTGAAAGG CTCAGGAAAT
    GAACTGGAGC CACCTTCAGA CCTAGTAACA TCAGAGCCTA CACCACGGAG CTTCAGAGTC
    ACATGGAATC CACCTTCTCA CAGTGTAGAC CGATTTAGGG TGGAATATTA TCCAGTGTCT
    GGTGGTCGAC CTCTGGAGGT ATTTGTTGGC AGATCAGATA GAACTACGGT TTTGACTAAT
    TTAAAGCCAG AAACAGAGTA TGTTGTGAAA GTGTATTCTG TGTTAGAAGG AGAAAGCAGT
    GAACCATTAG AAGGCACAGA AACGACATTG CCCATCCCTT CAGTCAGAAA TCTTAACATA
    TATGATATAG GAACCAGAAC AATGAGAGTT CGATGGGAAG CTGCGCTTGG AGCTACTGGT
    TATTTATTGC AGTATGAGCC AGTAAACGCT ACTGTGCCTT CAACTGAAAA AGAGTTGAGA
    GTTGGTGCAT CGGTGACAGA TGTACTTCTG GAAGATTTAT TCCCCAACAC TGAGTACACC
    TTGACTGTAT ATGCACTTTT TGATGAGCTG ATCAGTGATC CTCTTACCTC ACAGGAAGTC
    ACTTTACCCA TTCAAGGCGT GGGGAACATT AGAGTGAGAG ATATCACTCA CAGCTCAATG
    AATGTCCTTT GGAGTGCTGC TCCTGGAAAT GTTCTCAAAT ATATAGTAAG ATATAAAACA
    GAGGAAGAAG AAGAATTCAA AGAGGTGGAA GTGGATGGAT CAAGGACAAG TACACGACTT
    ACTGACCTTT TCTCACAAAC TCTATATGAA ATCAGTTTAA CTGCAGTATA TACTACACAA
    GGAACTACAG CACCAGTACC AGCTCCAGCA AACCTCCGGT TTTCTGAAAT AGCAGTAACA
    AGTTTCCGAG GTACTTGGGA GCATGGGGCT CCAGATGTAG CTTTGTACAG GATAACGTGG
    GCACCAGTTG GAGCCATAGA AAAAAAGAAA GAGACAATTG TTGATGGTGA TATAAACACT
    TTGGTTTTTG ATGAACTGGA ACCAGACACA GAGTATGAGG TTTCAGTAAC TGCGATTTAC
    CCTGACGAAT CAGAGAGTGA AGATCTGATC GGTGTTGAAA GGACATTGCC TGAAATAATT
    TCTACAACAC CTGCCCTAAG GACTGGCCCT AGGAATCTAC AGGTGTTCAA TGCAACACAG
    AATAGTTTGA CTGTACGATG GGATCGTGCT ATGGGCGATG TGCAAAAATA CAATATCTAT
    TATAAGCCTG TTTCTGCTGC TGGTGCTGAA CAAATGACAA GTGTTGGTGG AAAGCAGAAC
    AGTGTTGTTC TTCAGCGACT GCAGCCAGAC ACTCCATACA ACATTGTTGT TTCATCAAGG
    TACATTGGAG ATGAGGAAGG AGGAAATATC TCAGGGAGAG GAAAAACAAA GCCCTTAAAT
    ACAGTGAAGA ATCTAAGAGT GTATGACCCA ACAACAAGCA CTTTAAATGT ACGTTGGGAA
    GCTGCAGAAG GAAATGTGCG GCGGTATAAA ATTTACTATG TACCAACTGC TGGAGGACCA
    GAGGAAACGG TGACAGTGCC CGGAAATACA ATCTATTCAG TTCTTAGGAA TCTTCAGTCT
    GATACATCAT ATACAGTAAC TGTTGTTCCT GTCTATTCTG AAGGAGAAGG AGGTCGTATG
    TCAGAAACAG GAAAGACTTT GGTGCGGGGA ACAGTTCGAA ATATTCAAGT ATACAACCCC
    ACAACAAACA CTTTGAATGT TCGCTGGGAG CCTGCGCCTG GACAGGTCCA GCAATATAAA
    GTCAACTATG CTCCACTTCT TGGAACAAGG CCTACTGAGT CTGTTGTAGT TCCTGGCAAT
    ACTCACAACG CGCTGCTGGA GCAACTTATT CCTGACACAC AGTACTCTGT AAATGTTGTG
    GCTCTTTATG CAGATGGTGA GGGGGATCCT AGCCCTGGTG AAGGAAAAAC ATTACCTCGC
    AGTGGGCCAA AAAATATTAG AGTTTTTGGA GAGACCACTA ACAGTCTCTC TGTGAACTGG
    GATCATGCTG ATGGACCAGT CCAACAATAC AGAATCATCT ATTCACCAAC TGTTGGAGAC
    CCCATTGATG AATATACAAC AGTGCCTGGT AGGAGAAATA ATGTGGTACT GCAGCCCCTT
    TTGTCTGACA CGCCATATAA AATTACTGTG GTGGCAGTTT ATGCTGATGG TGATGGAGGA
    CAACTGACAG GAAAAGGAAG GACAGTGGGT TTGATTGCAC CACGGAACCT TCAAGTGTCT
    GATGAATGGT ACACAAGATT TAGGGTTTCC TGGGATAGAA TACCATCTCC TGTTCTTGGA
    TACAAGCTGG TTTATCAGCC ACGTGATTCA GATGAATCAA TGGAAGTATT TGTCGGGGAT
    GTGAGCTCCT ACACTTTGCA GAACCTGTCT CCTGGCACCA CATATGATGT GAAAGTTTAC
    GCTCAGTATG ATACAGGATT AAGTGGGCCC CTATCAGGAC CTGGGACAAC TTTATACTTA
    AATGTAACCG ATTTGACAAG CTATCAGGTT TCATGGGACT CGTTCTGTAT AAGATGGACT
    CCTCACCGAT CAGCCACTTC CTACAGACTG AAACTGAATC CAGTTGATGG ATCTCGAGGT
    CAAGAAATAA CAGTAACAGG ATCAGAATCA TCACACTGTT TTAATGGACT GAACCAAGAT
    ACACAGTACA CAGCTACTGT TTTTGTGCAA ACACCTAACC TTGAAGGACG AGGGGTTTCA
    ATAGATGAGC GTACATTGAT CAAGCCTACA GAATCACCAA CGACACCACC TGCTCCTCCT
    CCACCCCCTA CAATTCCACC TGCTCGGGAG GTGTGCAAAG GGGCCAAAGC AGACATAGTA
    TTCCTCATTG ACAGTTCGTG GAGTATTGGT GATGATAACT ACAATAAAGT CCGTCAGTTT
    ATCTTCAATA CTATTGGAGC ATTCGATGTA ATCAACCCTT CTGGAATCCA GGTCTCTTTT
    GTGCAGTTCA GCGATGATGC CCAGACTGAG TTCAAGTTGA ACACTTATGC CGACAAATCA
    CAGGCTCTAG GAGCACTTGC CAATATCAGA TACAAAGGAG GAAACACAAA AACAGGAAAG
    GCTCTTAAAT TTGTCCACGA GAAAGTGATG ACAGTTGAGA ATGGAATGAG AAGATCTGTT
    CCTAGGGTTC TGGTAGTGGT GACTGATGGC AGATCTCAGG ATGATGTTAA GAAATCAGCC
    TTGGACATTC AGCAATCTGG TTTTAGTGTC TTTGTCATTG GTGTGGCTGA TGTGGATTAT
    AATGAGCTAA GAAACATTGG CAGTAAACCA AGCGAGCGCC ATGTTTTTAT AGTCGATGAC
    TTTGATGCTT TTGAGAAGAT TGAAGATAAC CTGATCAACT TTGTATGTGA GACTGCAACA
    TCCACTTGCC CACTTATTTA CCTGGAAGGA TACACTTCAC CAGGTTTCAG AATGCTGGAA
    GCATATAACC TGACAGAGAA AACTTTTGCC TCAGTACAAG GTGTCTCAAT GCAACCAGGA
    TCTTTCAACA GTTTCCCTGC ATACAACCTA CATAAAGATG CCTTCATTTC CCTGCCTACC
    TCAGAATTGC ACCCAGAAGG ATTACCGCCT TCATATACGA TTATATTGCT ATTCCGTCTG
    CTGCCAGAAA CTCCCAATGA GCCATTTTCT CTATGGCAAA TAACAGATAA AGGTTACAAG
    CCACAAGTAG GAGTAAATCT TGATCCATCT AAGAAAACAC TGTCGTTCTT TAATAAGGGA
    GCTGCTGGGG ATACTCAGAC AGTAACATTT GATGGGAATG AAGTGAAGAA ACTCTTTTAT
    GGAAGCTTCC ACAAGGTTCT CATTGTGGTT TCTCCATCAT ATGTCAGAAT TTATATTGAT
    TGTAGTGAAG TTGCTGAAAA AGAAATCAAA GAAGCTGGCA ATATTACAGC AGAAGGATAT
    GAAGTACTTG GGAAGACATT AAAGGGTGAT AAAAAATCTG CACCCTTCCT GCTCCAGATG
    TTTGACATTG TGTGTACTGT GACCTGGACA AGTCGAGATA GATGCTGTGA TATTCCTGCT
    ACAAGGGATG AAGCCAAATG TCCAGCTCTG CCCCATTCCT GTACTTGCGC ACAAGACAGC
    GTTGGAGCAC CAGGACCCCC AGGCCCATCC GGTATTCCCG GTAGTAAAGG ACCACGGGGT
    GACCGAGGTT TGGATGGACC AGTTGGCCCA CCAGGTCAGC GAGGAGAACA AGGTTTGCCT
    GGTCCACAAG GACCACCTGG ACCTCAAGGA CCACCTGGAC TTTCCATTCC TGGAGAACCT
    GGTCGTCAAG GAATAAAAGG AGATGCCGGT GACCCAGGAT TGCCAGGAAG AATGGGTTCC
    CCAGGGGTGA CAGGACCACC AGGGCCTATG GGTCCACCAG GCACAAGGGG TTTCACAGGA
    AAGGATGGTG CAGTAGGACC CAGAGGACCA CCAGGACCAA TGGGAGCACC TGGACCTCAG
    GGAGTGCCAG GTCCAAGTGG GAAACCAGGA AAGCAAGGAG ATAATGGATT GCCAGGTCCT
    CTTGGACAGA AAGGAGACAA GGGTGAGAGG GGAGACATGG CATCTCAGAA TATGATGAGA
    TCAGTAGCAA GACAAGTCTG TGAACAGATG ATAAATAGTC AAATGAGCAG ATTTAATCAG
    ATGTTGAACC AAATTCCAAA TGATTACTAT TCCAATCGCA ATCAGCCTGG TCCTCCAGGT
    CCTCCCGGTC CTCCAGGCAA CGCTGGCATG AGAGGAGAAC CTGGAGCTGC TGGCAGATCT
    GGATTCCCAG GAACACCTGG TCCTCAAGGA CCACCTGGAG AAAGAGGAAT ACCAGGAGAG
    AAAGGGGAGA GAGGGAACCC AGGAATTGGA TCCCAAGGAC CCAGGGGACT TCCAGGACCT
    CCAGGACCTC CAGGCGAATC CAGAACAGGA CCTCCAGGAT CCTCAGGCTC CACAGGACCT
    CCAGGACCTT CAGGTCGCCC CGGTAACCAA GGAATTAGAG GGCCTCCTGG TCCAGCTGGA
    TACTGTGATG CTTCAATGTG TGCAAGCATT CCTTACAATG GGCAAGGATA CCCAGAACCT
    TATCAGCCTG AAAGTGGACC GCAGGAGCCA GAGAATCAGC CTTACGTAGT ACCTATGCAG
    CAAGAGAGTC CAGATGAGGA TTTTGAGGAC TATGGACATG AATATGCAGA ACATATGCGA
    TGGAGGAGAT CCTTACCAAA GAAATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949815.1
    CDS153..6002
    Translation

    Target ORF information:

    RefSeq Version XM_018094326.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen type XII alpha 1 chain (col12a1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094326.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGGCCTGGA ATGCAAAACT AAAGCTGCAG CTGATATAGT GTTGCTGGTG
    GATGGCTCTT GGAGTATCGG CCGCCCAAAT TTTAGGACCG TCAGGAGTTT TATAGCACGA
    CTGGTTGAAG TCTTTGATAT TGGCTCAGAC AGAGTGCAGA TTGCCTTGGC CCAATACAGT
    GGGGACCCTC GATCTGAATG GCAACTCAAT GCTCATAGCA CAAAGAAGTC TTTAATGGAT
    GCCGTTGCAA ACCTTCCTTA CAAGGGGGGT AACACTCTTA CAGGCATGGC TCTGAATTTC
    ATCTTGCAAA ATAACTTCAA GGCAGAGGCA GGCATGAGAG CAAAGTCAAA GAAAATTGGT
    GTCTTAATCA CTGATGGAAA GTCACAAGAT GACATTGTTG CACCTTCCAA AAAGTTACGA
    GACCAGGGAA TAGACTTATT TGCAATTGGT ATCAAAAATG CTGATGAAAA TGAACTGAGG
    CAGATTGGCT CAGATCCAGA TGAGACTTAC GTATTTAACG TTGCCGATTT TTCTTTGCTT
    GTCAATATTG TTGATGACCT CACTACCAAT CTATGTAACA GTGTGAAAGG CTCAGGAAAT
    GAACTGGAGC CACCTTCAGA CCTAGTAACA TCAGAGCCTA CACCACGGAG CTTCAGAGTC
    ACATGGAATC CACCTTCTCA CAGTGTAGAC CGATTTAGGG TGGAATATTA TCCAGTGTCT
    GGTGGTCGAC CTCTGGAGGT ATTTGTTGGC AGATCAGATA GAACTACGGT TTTGACTAAT
    TTAAAGCCAG AAACAGAGTA TGTTGTGAAA GTGTATTCTG TGTTAGAAGG AGAAAGCAGT
    GAACCATTAG AAGGCACAGA AACGACATTG CCCATCCCTT CAGTCAGAAA TCTTAACATA
    TATGATATAG GAACCAGAAC AATGAGAGTT CGATGGGAAG CTGCGCTTGG AGCTACTGGT
    TATTTATTGC AGTATGAGCC AGTAAACGCT ACTGTGCCTT CAACTGAAAA AGAGTTGAGA
    GTTGGTGCAT CGGTGACAGA TGTACTTCTG GAAGATTTAT TCCCCAACAC TGAGTACACC
    TTGACTGTAT ATGCACTTTT TGATGAGCTG ATCAGTGATC CTCTTACCTC ACAGGAAGTC
    ACTTTACCCA TTCAAGGCGT GGGGAACATT AGAGTGAGAG ATATCACTCA CAGCTCAATG
    AATGTCCTTT GGAGTGCTGC TCCTGGAAAT GTTCTCAAAT ATATAGTAAG ATATAAAACA
    GAGGAAGAAG AAGAATTCAA AGAGGTGGAA GTGGATGGAT CAAGGACAAG TACACGACTT
    ACTGACCTTT TCTCACAAAC TCTATATGAA ATCAGTTTAA CTGCAGTATA TACTACACAA
    GGAACTACAG CACCAGTACC AGCTCCAGCA AACCTCCGGT TTTCTGAAAT AGCAGTAACA
    AGTTTCCGAG GTACTTGGGA GCATGGGGCT CCAGATGTAG CTTTGTACAG GATAACGTGG
    GCACCAGTTG GAGCCATAGA AAAAAAGAAA GAGACAATTG TTGATGGTGA TATAAACACT
    TTGGTTTTTG ATGAACTGGA ACCAGACACA GAGTATGAGG TTTCAGTAAC TGCGATTTAC
    CCTGACGAAT CAGAGAGTGA AGATCTGATC GGTGTTGAAA GGACATTGCC TGAAATAATT
    TCTACAACAC CTGCCCTAAG GACTGGCCCT AGGAATCTAC AGGTGTTCAA TGCAACACAG
    AATAGTTTGA CTGTACGATG GGATCGTGCT ATGGGCGATG TGCAAAAATA CAATATCTAT
    TATAAGCCTG TTTCTGCTGC TGGTGCTGAA CAAATGACAA GTGTTGGTGG AAAGCAGAAC
    AGTGTTGTTC TTCAGCGACT GCAGCCAGAC ACTCCATACA ACATTGTTGT TTCATCAAGG
    TACATTGGAG ATGAGGAAGG AGGAAATATC TCAGGGAGAG GAAAAACAAA GCCCTTAAAT
    ACAGTGAAGA ATCTAAGAGT GTATGACCCA ACAACAAGCA CTTTAAATGT ACGTTGGGAA
    GCTGCAGAAG GAAATGTGCG GCGGTATAAA ATTTACTATG TACCAACTGC TGGAGGACCA
    GAGGAAACGG TGACAGTGCC CGGAAATACA ATCTATTCAG TTCTTAGGAA TCTTCAGTCT
    GATACATCAT ATACAGTAAC TGTTGTTCCT GTCTATTCTG AAGGAGAAGG AGGTCGTATG
    TCAGAAACAG GAAAGACTTT GGTGCGGGGA ACAGTTCGAA ATATTCAAGT ATACAACCCC
    ACAACAAACA CTTTGAATGT TCGCTGGGAG CCTGCGCCTG GACAGGTCCA GCAATATAAA
    GTCAACTATG CTCCACTTCT TGGAACAAGG CCTACTGAGT CTGTTGTAGT TCCTGGCAAT
    ACTCACAACG CGCTGCTGGA GCAACTTATT CCTGACACAC AGTACTCTGT AAATGTTGTG
    GCTCTTTATG CAGATGGTGA GGGGGATCCT AGCCCTGGTG AAGGAAAAAC ATTACCTCGC
    AGTGGGCCAA AAAATATTAG AGTTTTTGGA GAGACCACTA ACAGTCTCTC TGTGAACTGG
    GATCATGCTG ATGGACCAGT CCAACAATAC AGAATCATCT ATTCACCAAC TGTTGGAGAC
    CCCATTGATG AATATACAAC AGTGCCTGGT AGGAGAAATA ATGTGGTACT GCAGCCCCTT
    TTGTCTGACA CGCCATATAA AATTACTGTG GTGGCAGTTT ATGCTGATGG TGATGGAGGA
    CAACTGACAG GAAAAGGAAG GACAGTGGGT TTGATTGCAC CACGGAACCT TCAAGTGTCT
    GATGAATGGT ACACAAGATT TAGGGTTTCC TGGGATAGAA TACCATCTCC TGTTCTTGGA
    TACAAGCTGG TTTATCAGCC ACGTGATTCA GATGAATCAA TGGAAGTATT TGTCGGGGAT
    GTGAGCTCCT ACACTTTGCA GAACCTGTCT CCTGGCACCA CATATGATGT GAAAGTTTAC
    GCTCAGTATG ATACAGGATT AAGTGGGCCC CTATCAGGAC CTGGGACAAC TTTATACTTA
    AATGTAACCG ATTTGACAAG CTATCAGGTT TCATGGGACT CGTTCTGTAT AAGATGGACT
    CCTCACCGAT CAGCCACTTC CTACAGACTG AAACTGAATC CAGTTGATGG ATCTCGAGGT
    CAAGAAATAA CAGTAACAGG ATCAGAATCA TCACACTGTT TTAATGGACT GAACCAAGAT
    ACACAGTACA CAGCTACTGT TTTTGTGCAA ACACCTAACC TTGAAGGACG AGGGGTTTCA
    ATAGATGAGC GTACATTGAT CAAGCCTACA GAATCACCAA CGACACCACC TGCTCCTCCT
    CCACCCCCTA CAATTCCACC TGCTCGGGAG GTGTGCAAAG GGGCCAAAGC AGACATAGTA
    TTCCTCATTG ACAGTTCGTG GAGTATTGGT GATGATAACT ACAATAAAGT CCGTCAGTTT
    ATCTTCAATA CTATTGGAGC ATTCGATGTA ATCAACCCTT CTGGAATCCA GGTCTCTTTT
    GTGCAGTTCA GCGATGATGC CCAGACTGAG TTCAAGTTGA ACACTTATGC CGACAAATCA
    CAGGCTCTAG GAGCACTTGC CAATATCAGA TACAAAGGAG GAAACACAAA AACAGGAAAG
    GCTCTTAAAT TTGTCCACGA GAAAGTGATG ACAGTTGAGA ATGGAATGAG AAGATCTGTT
    CCTAGGGTTC TGGTAGTGGT GACTGATGGC AGATCTCAGG ATGATGTTAA GAAATCAGCC
    TTGGACATTC AGCAATCTGG TTTTAGTGTC TTTGTCATTG GTGTGGCTGA TGTGGATTAT
    AATGAGCTAA GAAACATTGG CAGTAAACCA AGCGAGCGCC ATGTTTTTAT AGTCGATGAC
    TTTGATGCTT TTGAGAAGAT TGAAGATAAC CTGATCAACT TTGTATGTGA GACTGCAACA
    TCCACTTGCC CACTTATTTA CCTGGAAGGA TACACTTCAC CAGGTTTCAG AATGCTGGAA
    GCATATAACC TGACAGAGAA AACTTTTGCC TCAGTACAAG GTGTCTCAAT GCAACCAGGA
    TCTTTCAACA GTTTCCCTGC ATACAACCTA CATAAAGATG CCTTCATTTC CCTGCCTACC
    TCAGAATTGC ACCCAGAAGG ATTACCGCCT TCATATACGA TTATATTGCT ATTCCGTCTG
    CTGCCAGAAA CTCCCAATGA GCCATTTTCT CTATGGCAAA TAACAGATAA AGGTTACAAG
    CCACAAGTAG GAGTAAATCT TGATCCATCT AAGAAAACAC TGTCGTTCTT TAATAAGGGA
    GCTGCTGGGG ATACTCAGAC AGTAACATTT GATGGGAATG AAGTGAAGAA ACTCTTTTAT
    GGAAGCTTCC ACAAGGTTCT CATTGTGGTT TCTCCATCAT ATGTCAGAAT TTATATTGAT
    TGTAGTGAAG TTGCTGAAAA AGAAATCAAA GAAGCTGGCA ATATTACAGC AGAAGGATAT
    GAAGTACTTG GGAAGACATT AAAGGGTGAT AAAAAATCTG CACCCTTCCT GCTCCAGATG
    TTTGACATTG TGTGTACTGT GACCTGGACA AGTCGAGATA GATGCTGTGA TATTCCTGCT
    ACAAGGGATG AAGCCAAATG TCCAGCTCTG CCCCATTCCT GTACTTGCGC ACAAGACAGC
    GTTGGAGCAC CAGGACCCCC AGGCCCATCC GGTATTCCCG GTAGTAAAGG ACCACGGGGT
    GACCGAGGTT TGGATGGACC AGTTGGCCCA CCAGGTCAGC GAGGAGAACA AGGTTTGCCT
    GGTCCACAAG GACCACCTGG ACCTCAAGGA CCACCTGGAC TTTCCATTCC TGGAGAACCT
    GGTCGTCAAG GAATAAAAGG AGATGCCGGT GACCCAGGAT TGCCAGGAAG AATGGGTTCC
    CCAGGGGTGA CAGGACCACC AGGGCCTATG GGTCCACCAG GCACAAGGGG TTTCACAGGA
    AAGGATGGTG CAGTAGGACC CAGAGGACCA CCAGGACCAA TGGGAGCACC TGGACCTCAG
    GGAGTGCCAG GTCCAAGTGG GAAACCAGGA AAGCAAGGAG ATAATGGATT GCCAGGTCCT
    CTTGGACAGA AAGGAGACAA GGGTGAGAGG GGAGACATGG CATCTCAGAA TATGATGAGA
    TCAGTAGCAA GACAAGTCTG TGAACAGATG ATAAATAGTC AAATGAGCAG ATTTAATCAG
    ATGTTGAACC AAATTCCAAA TGATTACTAT TCCAATCGCA ATCAGCCTGG TCCTCCAGGT
    CCTCCCGGTC CTCCAGGCAA CGCTGGCATG AGAGGAGAAC CTGGAGCTGC TGGCAGATCT
    GGATTCCCAG GAACACCTGG TCCTCAAGGA CCACCTGGAG AAAGAGGAAT ACCAGGAGAG
    AAAGGGGAGA GAGGGAACCC AGGAATTGGA TCCCAAGGAC CCAGGGGACT TCCAGGACCT
    CCAGGACCTC CAGGCGAATC CAGAACAGGA CCTCCAGGAT CCTCAGGCTC CACAGGACCT
    CCAGGACCTT CAGGTCGCCC CGGTAACCAA GGAATTAGAG GGCCTCCTGG TCCAGCTGGA
    TACTGTGATG CTTCAATGTG TGCAAGCATT CCTTACAATG GGCAAGGATA CCCAGAACCT
    TATCAGCCTG AAAGTGGACC GCAGGAGCCA GAGAATCAGC CTTACGTAGT ACCTATGCAG
    CAAGAGAGTC CAGATGAGGA TTTTGAGGAC TATGGACATG AATATGCAGA ACATATGCGA
    TGGAGGAGAT CCTTACCAAA GAAATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa36220
    Clone ID Related Accession (Same CDS sequence) XM_018094326.1 , XM_012963500.1
    Accession Version XM_012963500.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGGCCTGGA ATGCAAAACT AAAGCTGCAG CTGATATAGT GTTGCTGGTG
    GATGGCTCTT GGAGTATCGG CCGCCCAAAT TTTAGGACCG TCAGGAGTTT TATAGCACGA
    CTGGTTGAAG TCTTTGATAT TGGCTCAGAC AGAGTGCAGA TTGCCTTGGC CCAATACAGT
    GGGGACCCTC GATCTGAATG GCAACTCAAT GCTCATAGCA CAAAGAAGTC TTTAATGGAT
    GCCGTTGCAA ACCTTCCTTA CAAGGGGGGT AACACTCTTA CAGGCATGGC TCTGAATTTC
    ATCTTGCAAA ATAACTTCAA GGCAGAGGCA GGCATGAGAG CAAAGTCAAA GAAAATTGGT
    GTCTTAATCA CTGATGGAAA GTCACAAGAT GACATTGTTG CACCTTCCAA AAAGTTACGA
    GACCAGGGAA TAGACTTATT TGCAATTGGT ATCAAAAATG CTGATGAAAA TGAACTGAGG
    CAGATTGGCT CAGATCCAGA TGAGACTTAC GTATTTAACG TTGCCGATTT TTCTTTGCTT
    GTCAATATTG TTGATGACCT CACTACCAAT CTATGTAACA GTGTGAAAGG CTCAGGAAAT
    GAACTGGAGC CACCTTCAGA CCTAGTAACA TCAGAGCCTA CACCACGGAG CTTCAGAGTC
    ACATGGAATC CACCTTCTCA CAGTGTAGAC CGATTTAGGG TGGAATATTA TCCAGTGTCT
    GGTGGTCGAC CTCTGGAGGT ATTTGTTGGC AGATCAGATA GAACTACGGT TTTGACTAAT
    TTAAAGCCAG AAACAGAGTA TGTTGTGAAA GTGTATTCTG TGTTAGAAGG AGAAAGCAGT
    GAACCATTAG AAGGCACAGA AACGACATTG CCCATCCCTT CAGTCAGAAA TCTTAACATA
    TATGATATAG GAACCAGAAC AATGAGAGTT CGATGGGAAG CTGCGCTTGG AGCTACTGGT
    TATTTATTGC AGTATGAGCC AGTAAACGCT ACTGTGCCTT CAACTGAAAA AGAGTTGAGA
    GTTGGTGCAT CGGTGACAGA TGTACTTCTG GAAGATTTAT TCCCCAACAC TGAGTACACC
    TTGACTGTAT ATGCACTTTT TGATGAGCTG ATCAGTGATC CTCTTACCTC ACAGGAAGTC
    ACTTTACCCA TTCAAGGCGT GGGGAACATT AGAGTGAGAG ATATCACTCA CAGCTCAATG
    AATGTCCTTT GGAGTGCTGC TCCTGGAAAT GTTCTCAAAT ATATAGTAAG ATATAAAACA
    GAGGAAGAAG AAGAATTCAA AGAGGTGGAA GTGGATGGAT CAAGGACAAG TACACGACTT
    ACTGACCTTT TCTCACAAAC TCTATATGAA ATCAGTTTAA CTGCAGTATA TACTACACAA
    GGAACTACAG CACCAGTACC AGCTCCAGCA AACCTCCGGT TTTCTGAAAT AGCAGTAACA
    AGTTTCCGAG GTACTTGGGA GCATGGGGCT CCAGATGTAG CTTTGTACAG GATAACGTGG
    GCACCAGTTG GAGCCATAGA AAAAAAGAAA GAGACAATTG TTGATGGTGA TATAAACACT
    TTGGTTTTTG ATGAACTGGA ACCAGACACA GAGTATGAGG TTTCAGTAAC TGCGATTTAC
    CCTGACGAAT CAGAGAGTGA AGATCTGATC GGTGTTGAAA GGACATTGCC TGAAATAATT
    TCTACAACAC CTGCCCTAAG GACTGGCCCT AGGAATCTAC AGGTGTTCAA TGCAACACAG
    AATAGTTTGA CTGTACGATG GGATCGTGCT ATGGGCGATG TGCAAAAATA CAATATCTAT
    TATAAGCCTG TTTCTGCTGC TGGTGCTGAA CAAATGACAA GTGTTGGTGG AAAGCAGAAC
    AGTGTTGTTC TTCAGCGACT GCAGCCAGAC ACTCCATACA ACATTGTTGT TTCATCAAGG
    TACATTGGAG ATGAGGAAGG AGGAAATATC TCAGGGAGAG GAAAAACAAA GCCCTTAAAT
    ACAGTGAAGA ATCTAAGAGT GTATGACCCA ACAACAAGCA CTTTAAATGT ACGTTGGGAA
    GCTGCAGAAG GAAATGTGCG GCGGTATAAA ATTTACTATG TACCAACTGC TGGAGGACCA
    GAGGAAACGG TGACAGTGCC CGGAAATACA ATCTATTCAG TTCTTAGGAA TCTTCAGTCT
    GATACATCAT ATACAGTAAC TGTTGTTCCT GTCTATTCTG AAGGAGAAGG AGGTCGTATG
    TCAGAAACAG GAAAGACTTT GGTGCGGGGA ACAGTTCGAA ATATTCAAGT ATACAACCCC
    ACAACAAACA CTTTGAATGT TCGCTGGGAG CCTGCGCCTG GACAGGTCCA GCAATATAAA
    GTCAACTATG CTCCACTTCT TGGAACAAGG CCTACTGAGT CTGTTGTAGT TCCTGGCAAT
    ACTCACAACG CGCTGCTGGA GCAACTTATT CCTGACACAC AGTACTCTGT AAATGTTGTG
    GCTCTTTATG CAGATGGTGA GGGGGATCCT AGCCCTGGTG AAGGAAAAAC ATTACCTCGC
    AGTGGGCCAA AAAATATTAG AGTTTTTGGA GAGACCACTA ACAGTCTCTC TGTGAACTGG
    GATCATGCTG ATGGACCAGT CCAACAATAC AGAATCATCT ATTCACCAAC TGTTGGAGAC
    CCCATTGATG AATATACAAC AGTGCCTGGT AGGAGAAATA ATGTGGTACT GCAGCCCCTT
    TTGTCTGACA CGCCATATAA AATTACTGTG GTGGCAGTTT ATGCTGATGG TGATGGAGGA
    CAACTGACAG GAAAAGGAAG GACAGTGGGT TTGATTGCAC CACGGAACCT TCAAGTGTCT
    GATGAATGGT ACACAAGATT TAGGGTTTCC TGGGATAGAA TACCATCTCC TGTTCTTGGA
    TACAAGCTGG TTTATCAGCC ACGTGATTCA GATGAATCAA TGGAAGTATT TGTCGGGGAT
    GTGAGCTCCT ACACTTTGCA GAACCTGTCT CCTGGCACCA CATATGATGT GAAAGTTTAC
    GCTCAGTATG ATACAGGATT AAGTGGGCCC CTATCAGGAC CTGGGACAAC TTTATACTTA
    AATGTAACCG ATTTGACAAG CTATCAGGTT TCATGGGACT CGTTCTGTAT AAGATGGACT
    CCTCACCGAT CAGCCACTTC CTACAGACTG AAACTGAATC CAGTTGATGG ATCTCGAGGT
    CAAGAAATAA CAGTAACAGG ATCAGAATCA TCACACTGTT TTAATGGACT GAACCAAGAT
    ACACAGTACA CAGCTACTGT TTTTGTGCAA ACACCTAACC TTGAAGGACG AGGGGTTTCA
    ATAGATGAGC GTACATTGAT CAAGCCTACA GAATCACCAA CGACACCACC TGCTCCTCCT
    CCACCCCCTA CAATTCCACC TGCTCGGGAG GTGTGCAAAG GGGCCAAAGC AGACATAGTA
    TTCCTCATTG ACAGTTCGTG GAGTATTGGT GATGATAACT ACAATAAAGT CCGTCAGTTT
    ATCTTCAATA CTATTGGAGC ATTCGATGTA ATCAACCCTT CTGGAATCCA GGTCTCTTTT
    GTGCAGTTCA GCGATGATGC CCAGACTGAG TTCAAGTTGA ACACTTATGC CGACAAATCA
    CAGGCTCTAG GAGCACTTGC CAATATCAGA TACAAAGGAG GAAACACAAA AACAGGAAAG
    GCTCTTAAAT TTGTCCACGA GAAAGTGATG ACAGTTGAGA ATGGAATGAG AAGATCTGTT
    CCTAGGGTTC TGGTAGTGGT GACTGATGGC AGATCTCAGG ATGATGTTAA GAAATCAGCC
    TTGGACATTC AGCAATCTGG TTTTAGTGTC TTTGTCATTG GTGTGGCTGA TGTGGATTAT
    AATGAGCTAA GAAACATTGG CAGTAAACCA AGCGAGCGCC ATGTTTTTAT AGTCGATGAC
    TTTGATGCTT TTGAGAAGAT TGAAGATAAC CTGATCAACT TTGTATGTGA GACTGCAACA
    TCCACTTGCC CACTTATTTA CCTGGAAGGA TACACTTCAC CAGGTTTCAG AATGCTGGAA
    GCATATAACC TGACAGAGAA AACTTTTGCC TCAGTACAAG GTGTCTCAAT GCAACCAGGA
    TCTTTCAACA GTTTCCCTGC ATACAACCTA CATAAAGATG CCTTCATTTC CCTGCCTACC
    TCAGAATTGC ACCCAGAAGG ATTACCGCCT TCATATACGA TTATATTGCT ATTCCGTCTG
    CTGCCAGAAA CTCCCAATGA GCCATTTTCT CTATGGCAAA TAACAGATAA AGGTTACAAG
    CCACAAGTAG GAGTAAATCT TGATCCATCT AAGAAAACAC TGTCGTTCTT TAATAAGGGA
    GCTGCTGGGG ATACTCAGAC AGTAACATTT GATGGGAATG AAGTGAAGAA ACTCTTTTAT
    GGAAGCTTCC ACAAGGTTCT CATTGTGGTT TCTCCATCAT ATGTCAGAAT TTATATTGAT
    TGTAGTGAAG TTGCTGAAAA AGAAATCAAA GAAGCTGGCA ATATTACAGC AGAAGGATAT
    GAAGTACTTG GGAAGACATT AAAGGGTGAT AAAAAATCTG CACCCTTCCT GCTCCAGATG
    TTTGACATTG TGTGTACTGT GACCTGGACA AGTCGAGATA GATGCTGTGA TATTCCTGCT
    ACAAGGGATG AAGCCAAATG TCCAGCTCTG CCCCATTCCT GTACTTGCGC ACAAGACAGC
    GTTGGAGCAC CAGGACCCCC AGGCCCATCC GGTATTCCCG GTAGTAAAGG ACCACGGGGT
    GACCGAGGTT TGGATGGACC AGTTGGCCCA CCAGGTCAGC GAGGAGAACA AGGTTTGCCT
    GGTCCACAAG GACCACCTGG ACCTCAAGGA CCACCTGGAC TTTCCATTCC TGGAGAACCT
    GGTCGTCAAG GAATAAAAGG AGATGCCGGT GACCCAGGAT TGCCAGGAAG AATGGGTTCC
    CCAGGGGTGA CAGGACCACC AGGGCCTATG GGTCCACCAG GCACAAGGGG TTTCACAGGA
    AAGGATGGTG CAGTAGGACC CAGAGGACCA CCAGGACCAA TGGGAGCACC TGGACCTCAG
    GGAGTGCCAG GTCCAAGTGG GAAACCAGGA AAGCAAGGAG ATAATGGATT GCCAGGTCCT
    CTTGGACAGA AAGGAGACAA GGGTGAGAGG GGAGACATGG CATCTCAGAA TATGATGAGA
    TCAGTAGCAA GACAAGTCTG TGAACAGATG ATAAATAGTC AAATGAGCAG ATTTAATCAG
    ATGTTGAACC AAATTCCAAA TGATTACTAT TCCAATCGCA ATCAGCCTGG TCCTCCAGGT
    CCTCCCGGTC CTCCAGGCAA CGCTGGCATG AGAGGAGAAC CTGGAGCTGC TGGCAGATCT
    GGATTCCCAG GAACACCTGG TCCTCAAGGA CCACCTGGAG AAAGAGGAAT ACCAGGAGAG
    AAAGGGGAGA GAGGGAACCC AGGAATTGGA TCCCAAGGAC CCAGGGGACT TCCAGGACCT
    CCAGGACCTC CAGGCGAATC CAGAACAGGA CCTCCAGGAT CCTCAGGCTC CACAGGACCT
    CCAGGACCTT CAGGTCGCCC CGGTAACCAA GGAATTAGAG GGCCTCCTGG TCCAGCTGGA
    TACTGTGATG CTTCAATGTG TGCAAGCATT CCTTACAATG GGCAAGGATA CCCAGAACCT
    TATCAGCCTG AAAGTGGACC GCAGGAGCCA GAGAATCAGC CTTACGTAGT ACCTATGCAG
    CAAGAGAGTC CAGATGAGGA TTTTGAGGAC TATGGACATG AATATGCAGA ACATATGCGA
    TGGAGGAGAT CCTTACCAAA GAAATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818954.1
    CDS171..6020
    Translation

    Target ORF information:

    RefSeq Version XM_012963500.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen type XII alpha 1 chain (col12a1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963500.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGGCCTGGA ATGCAAAACT AAAGCTGCAG CTGATATAGT GTTGCTGGTG
    GATGGCTCTT GGAGTATCGG CCGCCCAAAT TTTAGGACCG TCAGGAGTTT TATAGCACGA
    CTGGTTGAAG TCTTTGATAT TGGCTCAGAC AGAGTGCAGA TTGCCTTGGC CCAATACAGT
    GGGGACCCTC GATCTGAATG GCAACTCAAT GCTCATAGCA CAAAGAAGTC TTTAATGGAT
    GCCGTTGCAA ACCTTCCTTA CAAGGGGGGT AACACTCTTA CAGGCATGGC TCTGAATTTC
    ATCTTGCAAA ATAACTTCAA GGCAGAGGCA GGCATGAGAG CAAAGTCAAA GAAAATTGGT
    GTCTTAATCA CTGATGGAAA GTCACAAGAT GACATTGTTG CACCTTCCAA AAAGTTACGA
    GACCAGGGAA TAGACTTATT TGCAATTGGT ATCAAAAATG CTGATGAAAA TGAACTGAGG
    CAGATTGGCT CAGATCCAGA TGAGACTTAC GTATTTAACG TTGCCGATTT TTCTTTGCTT
    GTCAATATTG TTGATGACCT CACTACCAAT CTATGTAACA GTGTGAAAGG CTCAGGAAAT
    GAACTGGAGC CACCTTCAGA CCTAGTAACA TCAGAGCCTA CACCACGGAG CTTCAGAGTC
    ACATGGAATC CACCTTCTCA CAGTGTAGAC CGATTTAGGG TGGAATATTA TCCAGTGTCT
    GGTGGTCGAC CTCTGGAGGT ATTTGTTGGC AGATCAGATA GAACTACGGT TTTGACTAAT
    TTAAAGCCAG AAACAGAGTA TGTTGTGAAA GTGTATTCTG TGTTAGAAGG AGAAAGCAGT
    GAACCATTAG AAGGCACAGA AACGACATTG CCCATCCCTT CAGTCAGAAA TCTTAACATA
    TATGATATAG GAACCAGAAC AATGAGAGTT CGATGGGAAG CTGCGCTTGG AGCTACTGGT
    TATTTATTGC AGTATGAGCC AGTAAACGCT ACTGTGCCTT CAACTGAAAA AGAGTTGAGA
    GTTGGTGCAT CGGTGACAGA TGTACTTCTG GAAGATTTAT TCCCCAACAC TGAGTACACC
    TTGACTGTAT ATGCACTTTT TGATGAGCTG ATCAGTGATC CTCTTACCTC ACAGGAAGTC
    ACTTTACCCA TTCAAGGCGT GGGGAACATT AGAGTGAGAG ATATCACTCA CAGCTCAATG
    AATGTCCTTT GGAGTGCTGC TCCTGGAAAT GTTCTCAAAT ATATAGTAAG ATATAAAACA
    GAGGAAGAAG AAGAATTCAA AGAGGTGGAA GTGGATGGAT CAAGGACAAG TACACGACTT
    ACTGACCTTT TCTCACAAAC TCTATATGAA ATCAGTTTAA CTGCAGTATA TACTACACAA
    GGAACTACAG CACCAGTACC AGCTCCAGCA AACCTCCGGT TTTCTGAAAT AGCAGTAACA
    AGTTTCCGAG GTACTTGGGA GCATGGGGCT CCAGATGTAG CTTTGTACAG GATAACGTGG
    GCACCAGTTG GAGCCATAGA AAAAAAGAAA GAGACAATTG TTGATGGTGA TATAAACACT
    TTGGTTTTTG ATGAACTGGA ACCAGACACA GAGTATGAGG TTTCAGTAAC TGCGATTTAC
    CCTGACGAAT CAGAGAGTGA AGATCTGATC GGTGTTGAAA GGACATTGCC TGAAATAATT
    TCTACAACAC CTGCCCTAAG GACTGGCCCT AGGAATCTAC AGGTGTTCAA TGCAACACAG
    AATAGTTTGA CTGTACGATG GGATCGTGCT ATGGGCGATG TGCAAAAATA CAATATCTAT
    TATAAGCCTG TTTCTGCTGC TGGTGCTGAA CAAATGACAA GTGTTGGTGG AAAGCAGAAC
    AGTGTTGTTC TTCAGCGACT GCAGCCAGAC ACTCCATACA ACATTGTTGT TTCATCAAGG
    TACATTGGAG ATGAGGAAGG AGGAAATATC TCAGGGAGAG GAAAAACAAA GCCCTTAAAT
    ACAGTGAAGA ATCTAAGAGT GTATGACCCA ACAACAAGCA CTTTAAATGT ACGTTGGGAA
    GCTGCAGAAG GAAATGTGCG GCGGTATAAA ATTTACTATG TACCAACTGC TGGAGGACCA
    GAGGAAACGG TGACAGTGCC CGGAAATACA ATCTATTCAG TTCTTAGGAA TCTTCAGTCT
    GATACATCAT ATACAGTAAC TGTTGTTCCT GTCTATTCTG AAGGAGAAGG AGGTCGTATG
    TCAGAAACAG GAAAGACTTT GGTGCGGGGA ACAGTTCGAA ATATTCAAGT ATACAACCCC
    ACAACAAACA CTTTGAATGT TCGCTGGGAG CCTGCGCCTG GACAGGTCCA GCAATATAAA
    GTCAACTATG CTCCACTTCT TGGAACAAGG CCTACTGAGT CTGTTGTAGT TCCTGGCAAT
    ACTCACAACG CGCTGCTGGA GCAACTTATT CCTGACACAC AGTACTCTGT AAATGTTGTG
    GCTCTTTATG CAGATGGTGA GGGGGATCCT AGCCCTGGTG AAGGAAAAAC ATTACCTCGC
    AGTGGGCCAA AAAATATTAG AGTTTTTGGA GAGACCACTA ACAGTCTCTC TGTGAACTGG
    GATCATGCTG ATGGACCAGT CCAACAATAC AGAATCATCT ATTCACCAAC TGTTGGAGAC
    CCCATTGATG AATATACAAC AGTGCCTGGT AGGAGAAATA ATGTGGTACT GCAGCCCCTT
    TTGTCTGACA CGCCATATAA AATTACTGTG GTGGCAGTTT ATGCTGATGG TGATGGAGGA
    CAACTGACAG GAAAAGGAAG GACAGTGGGT TTGATTGCAC CACGGAACCT TCAAGTGTCT
    GATGAATGGT ACACAAGATT TAGGGTTTCC TGGGATAGAA TACCATCTCC TGTTCTTGGA
    TACAAGCTGG TTTATCAGCC ACGTGATTCA GATGAATCAA TGGAAGTATT TGTCGGGGAT
    GTGAGCTCCT ACACTTTGCA GAACCTGTCT CCTGGCACCA CATATGATGT GAAAGTTTAC
    GCTCAGTATG ATACAGGATT AAGTGGGCCC CTATCAGGAC CTGGGACAAC TTTATACTTA
    AATGTAACCG ATTTGACAAG CTATCAGGTT TCATGGGACT CGTTCTGTAT AAGATGGACT
    CCTCACCGAT CAGCCACTTC CTACAGACTG AAACTGAATC CAGTTGATGG ATCTCGAGGT
    CAAGAAATAA CAGTAACAGG ATCAGAATCA TCACACTGTT TTAATGGACT GAACCAAGAT
    ACACAGTACA CAGCTACTGT TTTTGTGCAA ACACCTAACC TTGAAGGACG AGGGGTTTCA
    ATAGATGAGC GTACATTGAT CAAGCCTACA GAATCACCAA CGACACCACC TGCTCCTCCT
    CCACCCCCTA CAATTCCACC TGCTCGGGAG GTGTGCAAAG GGGCCAAAGC AGACATAGTA
    TTCCTCATTG ACAGTTCGTG GAGTATTGGT GATGATAACT ACAATAAAGT CCGTCAGTTT
    ATCTTCAATA CTATTGGAGC ATTCGATGTA ATCAACCCTT CTGGAATCCA GGTCTCTTTT
    GTGCAGTTCA GCGATGATGC CCAGACTGAG TTCAAGTTGA ACACTTATGC CGACAAATCA
    CAGGCTCTAG GAGCACTTGC CAATATCAGA TACAAAGGAG GAAACACAAA AACAGGAAAG
    GCTCTTAAAT TTGTCCACGA GAAAGTGATG ACAGTTGAGA ATGGAATGAG AAGATCTGTT
    CCTAGGGTTC TGGTAGTGGT GACTGATGGC AGATCTCAGG ATGATGTTAA GAAATCAGCC
    TTGGACATTC AGCAATCTGG TTTTAGTGTC TTTGTCATTG GTGTGGCTGA TGTGGATTAT
    AATGAGCTAA GAAACATTGG CAGTAAACCA AGCGAGCGCC ATGTTTTTAT AGTCGATGAC
    TTTGATGCTT TTGAGAAGAT TGAAGATAAC CTGATCAACT TTGTATGTGA GACTGCAACA
    TCCACTTGCC CACTTATTTA CCTGGAAGGA TACACTTCAC CAGGTTTCAG AATGCTGGAA
    GCATATAACC TGACAGAGAA AACTTTTGCC TCAGTACAAG GTGTCTCAAT GCAACCAGGA
    TCTTTCAACA GTTTCCCTGC ATACAACCTA CATAAAGATG CCTTCATTTC CCTGCCTACC
    TCAGAATTGC ACCCAGAAGG ATTACCGCCT TCATATACGA TTATATTGCT ATTCCGTCTG
    CTGCCAGAAA CTCCCAATGA GCCATTTTCT CTATGGCAAA TAACAGATAA AGGTTACAAG
    CCACAAGTAG GAGTAAATCT TGATCCATCT AAGAAAACAC TGTCGTTCTT TAATAAGGGA
    GCTGCTGGGG ATACTCAGAC AGTAACATTT GATGGGAATG AAGTGAAGAA ACTCTTTTAT
    GGAAGCTTCC ACAAGGTTCT CATTGTGGTT TCTCCATCAT ATGTCAGAAT TTATATTGAT
    TGTAGTGAAG TTGCTGAAAA AGAAATCAAA GAAGCTGGCA ATATTACAGC AGAAGGATAT
    GAAGTACTTG GGAAGACATT AAAGGGTGAT AAAAAATCTG CACCCTTCCT GCTCCAGATG
    TTTGACATTG TGTGTACTGT GACCTGGACA AGTCGAGATA GATGCTGTGA TATTCCTGCT
    ACAAGGGATG AAGCCAAATG TCCAGCTCTG CCCCATTCCT GTACTTGCGC ACAAGACAGC
    GTTGGAGCAC CAGGACCCCC AGGCCCATCC GGTATTCCCG GTAGTAAAGG ACCACGGGGT
    GACCGAGGTT TGGATGGACC AGTTGGCCCA CCAGGTCAGC GAGGAGAACA AGGTTTGCCT
    GGTCCACAAG GACCACCTGG ACCTCAAGGA CCACCTGGAC TTTCCATTCC TGGAGAACCT
    GGTCGTCAAG GAATAAAAGG AGATGCCGGT GACCCAGGAT TGCCAGGAAG AATGGGTTCC
    CCAGGGGTGA CAGGACCACC AGGGCCTATG GGTCCACCAG GCACAAGGGG TTTCACAGGA
    AAGGATGGTG CAGTAGGACC CAGAGGACCA CCAGGACCAA TGGGAGCACC TGGACCTCAG
    GGAGTGCCAG GTCCAAGTGG GAAACCAGGA AAGCAAGGAG ATAATGGATT GCCAGGTCCT
    CTTGGACAGA AAGGAGACAA GGGTGAGAGG GGAGACATGG CATCTCAGAA TATGATGAGA
    TCAGTAGCAA GACAAGTCTG TGAACAGATG ATAAATAGTC AAATGAGCAG ATTTAATCAG
    ATGTTGAACC AAATTCCAAA TGATTACTAT TCCAATCGCA ATCAGCCTGG TCCTCCAGGT
    CCTCCCGGTC CTCCAGGCAA CGCTGGCATG AGAGGAGAAC CTGGAGCTGC TGGCAGATCT
    GGATTCCCAG GAACACCTGG TCCTCAAGGA CCACCTGGAG AAAGAGGAAT ACCAGGAGAG
    AAAGGGGAGA GAGGGAACCC AGGAATTGGA TCCCAAGGAC CCAGGGGACT TCCAGGACCT
    CCAGGACCTC CAGGCGAATC CAGAACAGGA CCTCCAGGAT CCTCAGGCTC CACAGGACCT
    CCAGGACCTT CAGGTCGCCC CGGTAACCAA GGAATTAGAG GGCCTCCTGG TCCAGCTGGA
    TACTGTGATG CTTCAATGTG TGCAAGCATT CCTTACAATG GGCAAGGATA CCCAGAACCT
    TATCAGCCTG AAAGTGGACC GCAGGAGCCA GAGAATCAGC CTTACGTAGT ACCTATGCAG
    CAAGAGAGTC CAGATGAGGA TTTTGAGGAC TATGGACATG AATATGCAGA ACATATGCGA
    TGGAGGAGAT CCTTACCAAA GAAATTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44710
    Clone ID Related Accession (Same CDS sequence) XM_018094325.1
    Accession Version XM_018094325.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGACTGTTA CGGAAATTTC TTCCAAATCA ATGAAGATCA CCTGGAGACC ATCGCCAAGC 
    CAAATTACAG GCTACAGGAT TCAGCTTCTT CCAATGTTAG CGGGAAGTAA ACAACATTCA
    CTGAACCTTG GCCCTCAAAC TACAAGTATA AATGTAAAAG ATCTCTCCCC TGATACAGAA
    TACCAAATAA ATTTATATGC CATGAAAGGA CTCACAGCAA GTGAGCCAAT CTCAACACTG
    GAAAAAACCC AGGCTGTGAA AATTAAAGTC GAATGTTCCG GTGGTGTCGA TATAAAAGCA
    GATGTCGTTC TGCTTGTTGA TGGCTCCTAC AGTATTGGTG TAGCTAATTT TGCCAAAGTT
    AGAGCCTTCC TTGAAGTATT AGTAAAAAGC TTTGATATTT CGCCCAGCAA AGTACAGATC
    AGTCTTGTGC AATACAGTAG AGATCCATTT ACAGAGTTTA CCTTAAACAG GTATGACTCA
    ATTGAAGACA TTCTCAAAGC TGTAAATACC TTTCCATATA GAGGTGGTTC TACCAACACT
    GGCAAAGCAA TGACCTACGT GAGAGAAAAG GTCTTTGTTG AAATTAAAGG AGCAAGACCA
    AACGTCCCTA GAGTCATGAT TCTTATCACC GATGGAAAGT CATCAGATGC CTTTAAAGAT
    CCAGCTATAA AACTGCGGAA TTCAGATGTT GAGATTTTTG CAGTTGGTGT GAAGGATGCA
    GTGAGATCTG AGCTGGAGGC CATTGCTTCT CCTCCTTCAG ACACCCATGT GTACACAGTG
    GAAGATTTTG ATGCTTTTCA AAGGATTTCT TTTGAGCTAA CACAGTCTAT CTGCCTTAGA
    ATTGAGCAAG AGCTACAAAC AATAAAACGT AAATCTTTGA CCCCTGCAAG AGATTTGGCT
    TTCTCTGAGG TTACATCCAA CAGTTTTCGT GTGAACTGGT CTCCTGCGGC TGAGGATGTT
    TTGTCATACC TTGTTAAATA TAAGGTGGCT GTTGGAGAAG GTGAAGAATT CATCGTTTCC
    GTGCCAGCAC CAAAAACAAC CACAGTTCTT TCAAATTTAC TTCCAGAAAC TACTTATGCT
    GTGGCTGTCC TGGCAGAATA CGAGGAAGGA GAGAGTCCTC CATTAAATGG GGAAGAAACA
    ACTTTAGAAG TTACTGGCCC ACCAAGAAAC CTGAGAGTAA GTGACGAAAC AACTGATAGC
    TTTAAAGTTG GCTGGTCACC AGCTCCTGGC AATGTACTGA GGTACAGAAT TGCCTACCGG
    CCTGTGGCAG GAGGGGCAAG AAGAGAGGTT ACAGTCCCAG GAAGGGAGAC CGTGACAACA
    CTCCAGAACC TCACACCTGA TACAAAGTAC CAAGTATCTG TGATTCCTGA ATATCATTCG
    GGTCCTGGAA AACCTTTGAC AGGAAATGGA GCCACAGAAG AAGTGTTGGG AGAACCAAGG
    AATCTGAGGG TTTCTGATGC CACAACCTCC AGTTTAAAAC TGTCCTGGGA TTCGGCACCA
    GGCAAAGTGT TACAGTATCT TGTAACTTAC AACCCTTCTG CAGAAGGGGA AACTAAAGAG
    GTTACAGTAA GAGGAAATAC AACCAGTACA GTACTGAAGG GATTAGACCC AAGCACCACA
    TACTCCCTTT CTGTAGCTGC ACTCTATAGA TCTGGCGCTG GCTCATTACT TACTGGGGAA
    GGAACAACTC TTGATGAACG TGGTTCTCCT CGTAACCTGG TAACCAAAGA CATTACAGAC
    ACTACAATTG GAGCATCATG GACAGCGGCC CCAGGGATGG TTCGTGGTTA CAGAATTGCA
    TGGAAGTCTC TTTTTGATGA TGACTCTGGA GAAAAGAATG TTCCTGGTGG TATTACTAAC
    ACTGTGCTGG AAAACCTGAG GCCAGAAACA AAGTATAAAA TTTCAGTGTT TGCTAATTAT
    AGAAGTGGAG AAGGAGACCC TCTTGAAGGA GAAGCCACCA CTGAAGCGTC ACCAAGTGCC
    AAGCTGCTTA AAGTCAGTGA TGAGACAGAA ACTACAATGA AGGTAACCTG GCAGCCTGCC
    CCAGGAAATG TTGCAAATTA TCGGGTTATA TATCGGCCGA AGCTTGGAGG AAGGCAAGTG
    GTCGCTAAAG TGCCAGCTAC AGTCTCCTCA ACAGTACTGA AAAGGCTTCA ACCTCAAACA
    ACCTATGATA TTACAGTTGT TCCTGTTTAT AAGGCAGGGG ATGGAAAACC AAGACAAGGA
    GAAGGGACAA CAGCTTCTCC TTTCAAGCCC CCAAGGAATC TGAAAACATC TGACCCCACA
    ATCTCAACTT TCCGAGTCAC CTGGGAACCT GCTCCTGGTG AAGTTAGGGG TTATAAAATC
    ACATATTTTC CTTTTGGAGA GGAGCAATAT TTGGGAGAGT TAGTAGTTGG ACCATATGAT
    AACACTGTGG TACTAGAAGA ACTCAGGGCA GGAACATCTT ATAAAGTCAA TGTATTTGGA
    GTTTTTGAAA AAGGAGAGAG TGTACCTTTA ATTGGACAAG AAATGACAAC ATTGTCAGAT
    GCTCCTGAAG TTCGCATTGA TTCGTCAGGC CTGGAATGCA AAACTAAAGC TGCAGCTGAT
    ATAGTGTTGC TGGTGGATGG CTCTTGGAGT ATCGGCCGCC CAAATTTTAG GACCGTCAGG
    AGTTTTATAG CACGACTGGT TGAAGTCTTT GATATTGGCT CAGACAGAGT GCAGATTGCC
    TTGGCCCAAT ACAGTGGGGA CCCTCGATCT GAATGGCAAC TCAATGCTCA TAGCACAAAG
    AAGTCTTTAA TGGATGCCGT TGCAAACCTT CCTTACAAGG GGGGTAACAC TCTTACAGGC
    ATGGCTCTGA ATTTCATCTT GCAAAATAAC TTCAAGGCAG AGGCAGGCAT GAGAGCAAAG
    TCAAAGAAAA TTGGTGTCTT AATCACTGAT GGAAAGTCAC AAGATGACAT TGTTGCACCT
    TCCAAAAAGT TACGAGACCA GGGAATAGAC TTATTTGCAA TTGGTATCAA AAATGCTGAT
    GAAAATGAAC TGAGGCAGAT TGGCTCAGAT CCAGATGAGA CTTACGTATT TAACGTTGCC
    GATTTTTCTT TGCTTGTCAA TATTGTTGAT GACCTCACTA CCAATCTATG TAACAGTGTG
    AAAGGCTCAG GAAATGAACT GGAGCCACCT TCAGACCTAG TAACATCAGA GCCTACACCA
    CGGAGCTTCA GAGTCACATG GAATCCACCT TCTCACAGTG TAGACCGATT TAGGGTGGAA
    TATTATCCAG TGTCTGGTGG TCGACCTCTG GAGGTATTTG TTGGCAGATC AGATAGAACT
    ACGGTTTTGA CTAATTTAAA GCCAGAAACA GAGTATGTTG TGAAAGTGTA TTCTGTGTTA
    GAAGGAGAAA GCAGTGAACC ATTAGAAGGC ACAGAAACGA CATTGCCCAT CCCTTCAGTC
    AGAAATCTTA ACATATATGA TATAGGAACC AGAACAATGA GAGTTCGATG GGAAGCTGCG
    CTTGGAGCTA CTGGTTATTT ATTGCAGTAT GAGCCAGTAA ACGCTACTGT GCCTTCAACT
    GAAAAAGAGT TGAGAGTTGG TGCATCGGTG ACAGATGTAC TTCTGGAAGA TTTATTCCCC
    AACACTGAGT ACACCTTGAC TGTATATGCA CTTTTTGATG AGCTGATCAG TGATCCTCTT
    ACCTCACAGG AAGTCACTTT ACCCATTCAA GGCGTGGGGA ACATTAGAGT GAGAGATATC
    ACTCACAGCT CAATGAATGT CCTTTGGAGT GCTGCTCCTG GAAATGTTCT CAAATATATA
    GTAAGATATA AAACAGAGGA AGAAGAAGAA TTCAAAGAGG TGGAAGTGGA TGGATCAAGG
    ACAAGTACAC GACTTACTGA CCTTTTCTCA CAAACTCTAT ATGAAATCAG TTTAACTGCA
    GTATATACTA CACAAGGAAC TACAGCACCA GTACCAGCTC CAGCAAACCT CCGGTTTTCT
    GAAATAGCAG TAACAAGTTT CCGAGGTACT TGGGAGCATG GGGCTCCAGA TGTAGCTTTG
    TACAGGATAA CGTGGGCACC AGTTGGAGCC ATAGAAAAAA AGAAAGAGAC AATTGTTGAT
    GGTGATATAA ACACTTTGGT TTTTGATGAA CTGGAACCAG ACACAGAGTA TGAGGTTTCA
    GTAACTGCGA TTTACCCTGA CGAATCAGAG AGTGAAGATC TGATCGGTGT TGAAAGGACA
    TTGCCTGAAA TAATTTCTAC AACACCTGCC CTAAGGACTG GCCCTAGGAA TCTACAGGTG
    TTCAATGCAA CACAGAATAG TTTGACTGTA CGATGGGATC GTGCTATGGG CGATGTGCAA
    AAATACAATA TCTATTATAA GCCTGTTTCT GCTGCTGGTG CTGAACAAAT GACAAGTGTT
    GGTGGAAAGC AGAACAGTGT TGTTCTTCAG CGACTGCAGC CAGACACTCC ATACAACATT
    GTTGTTTCAT CAAGGTACAT TGGAGATGAG GAAGGAGGAA ATATCTCAGG GAGAGGAAAA
    ACAAAGCCCT TAAATACAGT GAAGAATCTA AGAGTGTATG ACCCAACAAC AAGCACTTTA
    AATGTACGTT GGGAAGCTGC AGAAGGAAAT GTGCGGCGGT ATAAAATTTA CTATGTACCA
    ACTGCTGGAG GACCAGAGGA AACGGTGACA GTGCCCGGAA ATACAATCTA TTCAGTTCTT
    AGGAATCTTC AGTCTGATAC ATCATATACA GTAACTGTTG TTCCTGTCTA TTCTGAAGGA
    GAAGGAGGTC GTATGTCAGA AACAGGAAAG ACTTTGGTGC GGGGAACAGT TCGAAATATT
    CAAGTATACA ACCCCACAAC AAACACTTTG AATGTTCGCT GGGAGCCTGC GCCTGGACAG
    GTCCAGCAAT ATAAAGTCAA CTATGCTCCA CTTCTTGGAA CAAGGCCTAC TGAGTCTGTT
    GTAGTTCCTG GCAATACTCA CAACGCGCTG CTGGAGCAAC TTATTCCTGA CACACAGTAC
    TCTGTAAATG TTGTGGCTCT TTATGCAGAT GGTGAGGGGG ATCCTAGCCC TGGTGAAGGA
    AAAACATTAC CTCGCAGTGG GCCAAAAAAT ATTAGAGTTT TTGGAGAGAC CACTAACAGT
    CTCTCTGTGA ACTGGGATCA TGCTGATGGA CCAGTCCAAC AATACAGAAT CATCTATTCA
    CCAACTGTTG GAGACCCCAT TGATGAATAT ACAACAGTGC CTGGTAGGAG AAATAATGTG
    GTACTGCAGC CCCTTTTGTC TGACACGCCA TATAAAATTA CTGTGGTGGC AGTTTATGCT
    GATGGTGATG GAGGACAACT GACAGGAAAA GGAAGGACAG TGGGTTTGAT TGCACCACGG
    AACCTTCAAG TGTCTGATGA ATGGTACACA AGATTTAGGG TTTCCTGGGA TAGAATACCA
    TCTCCTGTTC TTGGATACAA GCTGGTTTAT CAGCCACGTG ATTCAGATGA ATCAATGGAA
    GTATTTGTCG GGGATGTGAG CTCCTACACT TTGCAGAACC TGTCTCCTGG CACCACATAT
    GATGTGAAAG TTTACGCTCA GTATGATACA GGATTAAGTG GGCCCCTATC AGGACCTGGG
    ACAACTTTAT ACTTAAATGT AACCGATTTG ACAAGCTATC AGGTTTCATG GGACTCGTTC
    TGTATAAGAT GGACTCCTCA CCGATCAGCC ACTTCCTACA GACTGAAACT GAATCCAGTT
    GATGGATCTC GAGGTCAAGA AATAACAGTA ACAGGATCAG AATCATCACA CTGTTTTAAT
    GGACTGAACC AAGATACACA GTACACAGCT ACTGTTTTTG TGCAAACACC TAACCTTGAA
    GGACGAGGGG TTTCAATAGA TGAGCGTACA TTGATCAAGC CTACAGAATC ACCAACGACA
    CCACCTGCTC CTCCTCCACC CCCTACAATT CCACCTGCTC GGGAGGTGTG CAAAGGGGCC
    AAAGCAGACA TAGTATTCCT CATTGACAGT TCGTGGAGTA TTGGTGATGA TAACTACAAT
    AAAGTCCGTC AGTTTATCTT CAATACTATT GGAGCATTCG ATGTAATCAA CCCTTCTGGA
    ATCCAGGTCT CTTTTGTGCA GTTCAGCGAT GATGCCCAGA CTGAGTTCAA GTTGAACACT
    TATGCCGACA AATCACAGGC TCTAGGAGCA CTTGCCAATA TCAGATACAA AGGAGGAAAC
    ACAAAAACAG GAAAGGCTCT TAAATTTGTC CACGAGAAAG TGATGACAGT TGAGAATGGA
    ATGAGAAGAT CTGTTCCTAG GGTTCTGGTA GTGGTGACTG ATGGCAGATC TCAGGATGAT
    GTTAAGAAAT CAGCCTTGGA CATTCAGCAA TCTGGTTTTA GTGTCTTTGT CATTGGTGTG
    GCTGATGTGG ATTATAATGA GCTAAGAAAC ATTGGCAGTA AACCAAGCGA GCGCCATGTT
    TTTATAGTCG ATGACTTTGA TGCTTTTGAG AAGATTGAAG ATAACCTGAT CAACTTTGTA
    TGTGAGACTG CAACATCCAC TTGCCCACTT ATTTACCTGG AAGGATACAC TTCACCAGGT
    TTCAGAATGC TGGAAGCATA TAACCTGACA GAGAAAACTT TTGCCTCAGT ACAAGGTGTC
    TCAATGCAAC CAGGATCTTT CAACAGTTTC CCTGCATACA ACCTACATAA AGATGCCTTC
    ATTTCCCTGC CTACCTCAGA ATTGCACCCA GAAGGATTAC CGCCTTCATA TACGATTATA
    TTGCTATTCC GTCTGCTGCC AGAAACTCCC AATGAGCCAT TTTCTCTATG GCAAATAACA
    GATAAAGGTT ACAAGCCACA AGTAGGAGTA AATCTTGATC CATCTAAGAA AACACTGTCG
    TTCTTTAATA AGGGAGCTGC TGGGGATACT CAGACAGTAA CATTTGATGG GAATGAAGTG
    AAGAAACTCT TTTATGGAAG CTTCCACAAG GTTCTCATTG TGGTTTCTCC ATCATATGTC
    AGAATTTATA TTGATTGTAG TGAAGTTGCT GAAAAAGAAA TCAAAGAAGC TGGCAATATT
    ACAGCAGAAG GATATGAAGT ACTTGGGAAG ACATTAAAGG GTGATAAAAA ATCTGCACCC
    TTCCTGCTCC AGATGTTTGA CATTGTGTGT ACTGTGACCT GGACAAGTCG AGATAGATGC
    TGTGATATTC CTGCTACAAG GGATGAAGCC AAATGTCCAG CTCTGCCCCA TTCCTGTACT
    TGCGCACAAG ACAGCGTTGG AGCACCAGGA CCCCCAGGCC CATCCGGTAT TCCCGGTAGT
    AAAGGACCAC GGGGTGACCG AGGTTTGGAT GGACCAGTTG GCCCACCAGG TCAGCGAGGA
    GAACAAGGTT TGCCTGGTCC ACAAGGACCA CCTGGACCTC AAGGACCACC TGGACTTTCC
    ATTCCTGGAG AACCTGGTCG TCAAGGAATA AAAGGAGATG CCGGTGACCC AGGATTGCCA
    GGAAGAATGG GTTCCCCAGG GGTGACAGGA CCACCAGGGC CTATGGGTCC ACCAGGCACA
    AGGGGTTTCA CAGGAAAGGA TGGTGCAGTA GGACCCAGAG GACCACCAGG ACCAATGGGA
    GCACCTGGAC CTCAGGGAGT GCCAGGTCCA AGTGGGAAAC CAGGAAAGCA AGGAGATAAT
    GGATTGCCAG GTCCTCTTGG ACAGAAAGGA GACAAGGGTG AGAGGGGAGA CATGGCATCT
    CAGAATATGA TGAGATCAGT AGCAAGACAA GTCTGTGAAC AGATGATAAA TAGTCAAATG
    AGCAGATTTA ATCAGATGTT GAACCAAATT CCAAATGATT ACTATTCCAA TCGCAATCAG
    CCTGGTCCTC CAGGTCCTCC CGGTCCTCCA GGCAACGCTG GCATGAGAGG AGAACCTGGA
    GCTGCTGGCA GATCTGGATT CCCAGGAACA CCTGGTCCTC AAGGACCACC TGGAGAAAGA
    GGAATACCAG GAGAGAAAGG GGAGAGAGGG AACCCAGGAA TTGGATCCCA AGGACCCAGG
    GGACTTCCAG GACCTCCAGG ACCTCCAGGC GAATCCAGAA CAGGACCTCC AGGATCCTCA
    GGCTCCACAG GACCTCCAGG ACCTTCAGGT CGCCCCGGTA ACCAAGGAAT TAGAGGGCCT
    CCTGGTCCAG CTGGATACTG TGATGCTTCA ATGTGTGCAA GCATTCCTTA CAATGGGCAA
    GGATACCCAG GTTCCGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949814.1
    CDS77..8257
    Translation

    Target ORF information:

    RefSeq Version XM_018094325.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen type XII alpha 1 chain (col12a1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094325.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGACTGTTA CGGAAATTTC TTCCAAATCA ATGAAGATCA CCTGGAGACC ATCGCCAAGC 
    CAAATTACAG GCTACAGGAT TCAGCTTCTT CCAATGTTAG CGGGAAGTAA ACAACATTCA
    CTGAACCTTG GCCCTCAAAC TACAAGTATA AATGTAAAAG ATCTCTCCCC TGATACAGAA
    TACCAAATAA ATTTATATGC CATGAAAGGA CTCACAGCAA GTGAGCCAAT CTCAACACTG
    GAAAAAACCC AGGCTGTGAA AATTAAAGTC GAATGTTCCG GTGGTGTCGA TATAAAAGCA
    GATGTCGTTC TGCTTGTTGA TGGCTCCTAC AGTATTGGTG TAGCTAATTT TGCCAAAGTT
    AGAGCCTTCC TTGAAGTATT AGTAAAAAGC TTTGATATTT CGCCCAGCAA AGTACAGATC
    AGTCTTGTGC AATACAGTAG AGATCCATTT ACAGAGTTTA CCTTAAACAG GTATGACTCA
    ATTGAAGACA TTCTCAAAGC TGTAAATACC TTTCCATATA GAGGTGGTTC TACCAACACT
    GGCAAAGCAA TGACCTACGT GAGAGAAAAG GTCTTTGTTG AAATTAAAGG AGCAAGACCA
    AACGTCCCTA GAGTCATGAT TCTTATCACC GATGGAAAGT CATCAGATGC CTTTAAAGAT
    CCAGCTATAA AACTGCGGAA TTCAGATGTT GAGATTTTTG CAGTTGGTGT GAAGGATGCA
    GTGAGATCTG AGCTGGAGGC CATTGCTTCT CCTCCTTCAG ACACCCATGT GTACACAGTG
    GAAGATTTTG ATGCTTTTCA AAGGATTTCT TTTGAGCTAA CACAGTCTAT CTGCCTTAGA
    ATTGAGCAAG AGCTACAAAC AATAAAACGT AAATCTTTGA CCCCTGCAAG AGATTTGGCT
    TTCTCTGAGG TTACATCCAA CAGTTTTCGT GTGAACTGGT CTCCTGCGGC TGAGGATGTT
    TTGTCATACC TTGTTAAATA TAAGGTGGCT GTTGGAGAAG GTGAAGAATT CATCGTTTCC
    GTGCCAGCAC CAAAAACAAC CACAGTTCTT TCAAATTTAC TTCCAGAAAC TACTTATGCT
    GTGGCTGTCC TGGCAGAATA CGAGGAAGGA GAGAGTCCTC CATTAAATGG GGAAGAAACA
    ACTTTAGAAG TTACTGGCCC ACCAAGAAAC CTGAGAGTAA GTGACGAAAC AACTGATAGC
    TTTAAAGTTG GCTGGTCACC AGCTCCTGGC AATGTACTGA GGTACAGAAT TGCCTACCGG
    CCTGTGGCAG GAGGGGCAAG AAGAGAGGTT ACAGTCCCAG GAAGGGAGAC CGTGACAACA
    CTCCAGAACC TCACACCTGA TACAAAGTAC CAAGTATCTG TGATTCCTGA ATATCATTCG
    GGTCCTGGAA AACCTTTGAC AGGAAATGGA GCCACAGAAG AAGTGTTGGG AGAACCAAGG
    AATCTGAGGG TTTCTGATGC CACAACCTCC AGTTTAAAAC TGTCCTGGGA TTCGGCACCA
    GGCAAAGTGT TACAGTATCT TGTAACTTAC AACCCTTCTG CAGAAGGGGA AACTAAAGAG
    GTTACAGTAA GAGGAAATAC AACCAGTACA GTACTGAAGG GATTAGACCC AAGCACCACA
    TACTCCCTTT CTGTAGCTGC ACTCTATAGA TCTGGCGCTG GCTCATTACT TACTGGGGAA
    GGAACAACTC TTGATGAACG TGGTTCTCCT CGTAACCTGG TAACCAAAGA CATTACAGAC
    ACTACAATTG GAGCATCATG GACAGCGGCC CCAGGGATGG TTCGTGGTTA CAGAATTGCA
    TGGAAGTCTC TTTTTGATGA TGACTCTGGA GAAAAGAATG TTCCTGGTGG TATTACTAAC
    ACTGTGCTGG AAAACCTGAG GCCAGAAACA AAGTATAAAA TTTCAGTGTT TGCTAATTAT
    AGAAGTGGAG AAGGAGACCC TCTTGAAGGA GAAGCCACCA CTGAAGCGTC ACCAAGTGCC
    AAGCTGCTTA AAGTCAGTGA TGAGACAGAA ACTACAATGA AGGTAACCTG GCAGCCTGCC
    CCAGGAAATG TTGCAAATTA TCGGGTTATA TATCGGCCGA AGCTTGGAGG AAGGCAAGTG
    GTCGCTAAAG TGCCAGCTAC AGTCTCCTCA ACAGTACTGA AAAGGCTTCA ACCTCAAACA
    ACCTATGATA TTACAGTTGT TCCTGTTTAT AAGGCAGGGG ATGGAAAACC AAGACAAGGA
    GAAGGGACAA CAGCTTCTCC TTTCAAGCCC CCAAGGAATC TGAAAACATC TGACCCCACA
    ATCTCAACTT TCCGAGTCAC CTGGGAACCT GCTCCTGGTG AAGTTAGGGG TTATAAAATC
    ACATATTTTC CTTTTGGAGA GGAGCAATAT TTGGGAGAGT TAGTAGTTGG ACCATATGAT
    AACACTGTGG TACTAGAAGA ACTCAGGGCA GGAACATCTT ATAAAGTCAA TGTATTTGGA
    GTTTTTGAAA AAGGAGAGAG TGTACCTTTA ATTGGACAAG AAATGACAAC ATTGTCAGAT
    GCTCCTGAAG TTCGCATTGA TTCGTCAGGC CTGGAATGCA AAACTAAAGC TGCAGCTGAT
    ATAGTGTTGC TGGTGGATGG CTCTTGGAGT ATCGGCCGCC CAAATTTTAG GACCGTCAGG
    AGTTTTATAG CACGACTGGT TGAAGTCTTT GATATTGGCT CAGACAGAGT GCAGATTGCC
    TTGGCCCAAT ACAGTGGGGA CCCTCGATCT GAATGGCAAC TCAATGCTCA TAGCACAAAG
    AAGTCTTTAA TGGATGCCGT TGCAAACCTT CCTTACAAGG GGGGTAACAC TCTTACAGGC
    ATGGCTCTGA ATTTCATCTT GCAAAATAAC TTCAAGGCAG AGGCAGGCAT GAGAGCAAAG
    TCAAAGAAAA TTGGTGTCTT AATCACTGAT GGAAAGTCAC AAGATGACAT TGTTGCACCT
    TCCAAAAAGT TACGAGACCA GGGAATAGAC TTATTTGCAA TTGGTATCAA AAATGCTGAT
    GAAAATGAAC TGAGGCAGAT TGGCTCAGAT CCAGATGAGA CTTACGTATT TAACGTTGCC
    GATTTTTCTT TGCTTGTCAA TATTGTTGAT GACCTCACTA CCAATCTATG TAACAGTGTG
    AAAGGCTCAG GAAATGAACT GGAGCCACCT TCAGACCTAG TAACATCAGA GCCTACACCA
    CGGAGCTTCA GAGTCACATG GAATCCACCT TCTCACAGTG TAGACCGATT TAGGGTGGAA
    TATTATCCAG TGTCTGGTGG TCGACCTCTG GAGGTATTTG TTGGCAGATC AGATAGAACT
    ACGGTTTTGA CTAATTTAAA GCCAGAAACA GAGTATGTTG TGAAAGTGTA TTCTGTGTTA
    GAAGGAGAAA GCAGTGAACC ATTAGAAGGC ACAGAAACGA CATTGCCCAT CCCTTCAGTC
    AGAAATCTTA ACATATATGA TATAGGAACC AGAACAATGA GAGTTCGATG GGAAGCTGCG
    CTTGGAGCTA CTGGTTATTT ATTGCAGTAT GAGCCAGTAA ACGCTACTGT GCCTTCAACT
    GAAAAAGAGT TGAGAGTTGG TGCATCGGTG ACAGATGTAC TTCTGGAAGA TTTATTCCCC
    AACACTGAGT ACACCTTGAC TGTATATGCA CTTTTTGATG AGCTGATCAG TGATCCTCTT
    ACCTCACAGG AAGTCACTTT ACCCATTCAA GGCGTGGGGA ACATTAGAGT GAGAGATATC
    ACTCACAGCT CAATGAATGT CCTTTGGAGT GCTGCTCCTG GAAATGTTCT CAAATATATA
    GTAAGATATA AAACAGAGGA AGAAGAAGAA TTCAAAGAGG TGGAAGTGGA TGGATCAAGG
    ACAAGTACAC GACTTACTGA CCTTTTCTCA CAAACTCTAT ATGAAATCAG TTTAACTGCA
    GTATATACTA CACAAGGAAC TACAGCACCA GTACCAGCTC CAGCAAACCT CCGGTTTTCT
    GAAATAGCAG TAACAAGTTT CCGAGGTACT TGGGAGCATG GGGCTCCAGA TGTAGCTTTG
    TACAGGATAA CGTGGGCACC AGTTGGAGCC ATAGAAAAAA AGAAAGAGAC AATTGTTGAT
    GGTGATATAA ACACTTTGGT TTTTGATGAA CTGGAACCAG ACACAGAGTA TGAGGTTTCA
    GTAACTGCGA TTTACCCTGA CGAATCAGAG AGTGAAGATC TGATCGGTGT TGAAAGGACA
    TTGCCTGAAA TAATTTCTAC AACACCTGCC CTAAGGACTG GCCCTAGGAA TCTACAGGTG
    TTCAATGCAA CACAGAATAG TTTGACTGTA CGATGGGATC GTGCTATGGG CGATGTGCAA
    AAATACAATA TCTATTATAA GCCTGTTTCT GCTGCTGGTG CTGAACAAAT GACAAGTGTT
    GGTGGAAAGC AGAACAGTGT TGTTCTTCAG CGACTGCAGC CAGACACTCC ATACAACATT
    GTTGTTTCAT CAAGGTACAT TGGAGATGAG GAAGGAGGAA ATATCTCAGG GAGAGGAAAA
    ACAAAGCCCT TAAATACAGT GAAGAATCTA AGAGTGTATG ACCCAACAAC AAGCACTTTA
    AATGTACGTT GGGAAGCTGC AGAAGGAAAT GTGCGGCGGT ATAAAATTTA CTATGTACCA
    ACTGCTGGAG GACCAGAGGA AACGGTGACA GTGCCCGGAA ATACAATCTA TTCAGTTCTT
    AGGAATCTTC AGTCTGATAC ATCATATACA GTAACTGTTG TTCCTGTCTA TTCTGAAGGA
    GAAGGAGGTC GTATGTCAGA AACAGGAAAG ACTTTGGTGC GGGGAACAGT TCGAAATATT
    CAAGTATACA ACCCCACAAC AAACACTTTG AATGTTCGCT GGGAGCCTGC GCCTGGACAG
    GTCCAGCAAT ATAAAGTCAA CTATGCTCCA CTTCTTGGAA CAAGGCCTAC TGAGTCTGTT
    GTAGTTCCTG GCAATACTCA CAACGCGCTG CTGGAGCAAC TTATTCCTGA CACACAGTAC
    TCTGTAAATG TTGTGGCTCT TTATGCAGAT GGTGAGGGGG ATCCTAGCCC TGGTGAAGGA
    AAAACATTAC CTCGCAGTGG GCCAAAAAAT ATTAGAGTTT TTGGAGAGAC CACTAACAGT
    CTCTCTGTGA ACTGGGATCA TGCTGATGGA CCAGTCCAAC AATACAGAAT CATCTATTCA
    CCAACTGTTG GAGACCCCAT TGATGAATAT ACAACAGTGC CTGGTAGGAG AAATAATGTG
    GTACTGCAGC CCCTTTTGTC TGACACGCCA TATAAAATTA CTGTGGTGGC AGTTTATGCT
    GATGGTGATG GAGGACAACT GACAGGAAAA GGAAGGACAG TGGGTTTGAT TGCACCACGG
    AACCTTCAAG TGTCTGATGA ATGGTACACA AGATTTAGGG TTTCCTGGGA TAGAATACCA
    TCTCCTGTTC TTGGATACAA GCTGGTTTAT CAGCCACGTG ATTCAGATGA ATCAATGGAA
    GTATTTGTCG GGGATGTGAG CTCCTACACT TTGCAGAACC TGTCTCCTGG CACCACATAT
    GATGTGAAAG TTTACGCTCA GTATGATACA GGATTAAGTG GGCCCCTATC AGGACCTGGG
    ACAACTTTAT ACTTAAATGT AACCGATTTG ACAAGCTATC AGGTTTCATG GGACTCGTTC
    TGTATAAGAT GGACTCCTCA CCGATCAGCC ACTTCCTACA GACTGAAACT GAATCCAGTT
    GATGGATCTC GAGGTCAAGA AATAACAGTA ACAGGATCAG AATCATCACA CTGTTTTAAT
    GGACTGAACC AAGATACACA GTACACAGCT ACTGTTTTTG TGCAAACACC TAACCTTGAA
    GGACGAGGGG TTTCAATAGA TGAGCGTACA TTGATCAAGC CTACAGAATC ACCAACGACA
    CCACCTGCTC CTCCTCCACC CCCTACAATT CCACCTGCTC GGGAGGTGTG CAAAGGGGCC
    AAAGCAGACA TAGTATTCCT CATTGACAGT TCGTGGAGTA TTGGTGATGA TAACTACAAT
    AAAGTCCGTC AGTTTATCTT CAATACTATT GGAGCATTCG ATGTAATCAA CCCTTCTGGA
    ATCCAGGTCT CTTTTGTGCA GTTCAGCGAT GATGCCCAGA CTGAGTTCAA GTTGAACACT
    TATGCCGACA AATCACAGGC TCTAGGAGCA CTTGCCAATA TCAGATACAA AGGAGGAAAC
    ACAAAAACAG GAAAGGCTCT TAAATTTGTC CACGAGAAAG TGATGACAGT TGAGAATGGA
    ATGAGAAGAT CTGTTCCTAG GGTTCTGGTA GTGGTGACTG ATGGCAGATC TCAGGATGAT
    GTTAAGAAAT CAGCCTTGGA CATTCAGCAA TCTGGTTTTA GTGTCTTTGT CATTGGTGTG
    GCTGATGTGG ATTATAATGA GCTAAGAAAC ATTGGCAGTA AACCAAGCGA GCGCCATGTT
    TTTATAGTCG ATGACTTTGA TGCTTTTGAG AAGATTGAAG ATAACCTGAT CAACTTTGTA
    TGTGAGACTG CAACATCCAC TTGCCCACTT ATTTACCTGG AAGGATACAC TTCACCAGGT
    TTCAGAATGC TGGAAGCATA TAACCTGACA GAGAAAACTT TTGCCTCAGT ACAAGGTGTC
    TCAATGCAAC CAGGATCTTT CAACAGTTTC CCTGCATACA ACCTACATAA AGATGCCTTC
    ATTTCCCTGC CTACCTCAGA ATTGCACCCA GAAGGATTAC CGCCTTCATA TACGATTATA
    TTGCTATTCC GTCTGCTGCC AGAAACTCCC AATGAGCCAT TTTCTCTATG GCAAATAACA
    GATAAAGGTT ACAAGCCACA AGTAGGAGTA AATCTTGATC CATCTAAGAA AACACTGTCG
    TTCTTTAATA AGGGAGCTGC TGGGGATACT CAGACAGTAA CATTTGATGG GAATGAAGTG
    AAGAAACTCT TTTATGGAAG CTTCCACAAG GTTCTCATTG TGGTTTCTCC ATCATATGTC
    AGAATTTATA TTGATTGTAG TGAAGTTGCT GAAAAAGAAA TCAAAGAAGC TGGCAATATT
    ACAGCAGAAG GATATGAAGT ACTTGGGAAG ACATTAAAGG GTGATAAAAA ATCTGCACCC
    TTCCTGCTCC AGATGTTTGA CATTGTGTGT ACTGTGACCT GGACAAGTCG AGATAGATGC
    TGTGATATTC CTGCTACAAG GGATGAAGCC AAATGTCCAG CTCTGCCCCA TTCCTGTACT
    TGCGCACAAG ACAGCGTTGG AGCACCAGGA CCCCCAGGCC CATCCGGTAT TCCCGGTAGT
    AAAGGACCAC GGGGTGACCG AGGTTTGGAT GGACCAGTTG GCCCACCAGG TCAGCGAGGA
    GAACAAGGTT TGCCTGGTCC ACAAGGACCA CCTGGACCTC AAGGACCACC TGGACTTTCC
    ATTCCTGGAG AACCTGGTCG TCAAGGAATA AAAGGAGATG CCGGTGACCC AGGATTGCCA
    GGAAGAATGG GTTCCCCAGG GGTGACAGGA CCACCAGGGC CTATGGGTCC ACCAGGCACA
    AGGGGTTTCA CAGGAAAGGA TGGTGCAGTA GGACCCAGAG GACCACCAGG ACCAATGGGA
    GCACCTGGAC CTCAGGGAGT GCCAGGTCCA AGTGGGAAAC CAGGAAAGCA AGGAGATAAT
    GGATTGCCAG GTCCTCTTGG ACAGAAAGGA GACAAGGGTG AGAGGGGAGA CATGGCATCT
    CAGAATATGA TGAGATCAGT AGCAAGACAA GTCTGTGAAC AGATGATAAA TAGTCAAATG
    AGCAGATTTA ATCAGATGTT GAACCAAATT CCAAATGATT ACTATTCCAA TCGCAATCAG
    CCTGGTCCTC CAGGTCCTCC CGGTCCTCCA GGCAACGCTG GCATGAGAGG AGAACCTGGA
    GCTGCTGGCA GATCTGGATT CCCAGGAACA CCTGGTCCTC AAGGACCACC TGGAGAAAGA
    GGAATACCAG GAGAGAAAGG GGAGAGAGGG AACCCAGGAA TTGGATCCCA AGGACCCAGG
    GGACTTCCAG GACCTCCAGG ACCTCCAGGC GAATCCAGAA CAGGACCTCC AGGATCCTCA
    GGCTCCACAG GACCTCCAGG ACCTTCAGGT CGCCCCGGTA ACCAAGGAAT TAGAGGGCCT
    CCTGGTCCAG CTGGATACTG TGATGCTTCA ATGTGTGCAA GCATTCCTTA CAATGGGCAA
    GGATACCCAG GTTCCGGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58275
    Clone ID Related Accession (Same CDS sequence) XM_002936200.3 , XM_031901995.1
    Accession Version XM_002936200.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936200.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGTTGGTCC ACCTACAGAC TTAAATTTTG AAATAGTAAA TGAAAATACA
    GTTCGAATGT CATGGCAAAG GCCGGCAGAA AGAATAAAGG GTTACAAGAT TACTATCGTC
    CCTACAATTG ATGGACCAGA AAAGGAACTA GATCTTCCAG CTTCTGCTAC GCAAACCATA
    CTAACTGAAC TTGTACCAGA TGTGGAATAT GTTGTGACAG CTATTTCATA TGATGAATTA
    GAGGAGAGTG TACCTGTGTT TGGCCAACTT ACAATTCAAA CAGGAGGTCG AGTGACACTT
    GAAGAGCCTA AACTTGAGAC AAGTAAATCA CCACGCTGCT CTATTAGTTC TGTTGCTGAT
    GTAGTTTTCC TTGTGGATGG ATCATGGAGT GTTGGAAGAA ACAACTTCAA ATATATTCTC
    GATTTCATGG TTTCTCTGGT CTCAGCCTTT GATATTGGGG AAGACAAAAC CCGAGTCGGT
    GTTGTGCAGT ATAGCTCTGA CACTAGGACT GAGTTTAACC TGAACAGTTA TTACAAGAAA
    GAGGAATTAG TATCAGCCAT AAAAAGAATA CCATACAAGG GAGGCAATAC AATGACAGGT
    GATGCCCTTG ATTACCTGAT TAAGAACTCC TTTGTCAAAA CTGCCGGCTC AAGAAAAGGA
    TTTCCAAGAA TAGCTATCAT TATAACTGAT GGTAAATCCC AAGATGAAGT GGAGATTCCA
    GCAAGAGAGC TGCGTAGCTT AGGGGTTGAA GTTTTTTCCC TTGGTATAAA AGCAGCAGAT
    GCAAAAGAAC TCAAACAAAT AGCTTCTCTG CCTTCCTTGA AACATGTTTT TAATGTTGCT
    AATTTTGATT CGATTGTGGA AGTGCAAGAT GAAATAATTA CTCAAGTTTG CTCAGGAGTT
    GAAGAGCAGC TAAGTGAGCT TGTTAGTGGA GAAGAAGCTG TTGAGCCTCC TTCAGATATG
    ACTGTTACGG AAATTTCTTC CAAATCAATG AAGATCACCT GGAGACCATC GCCAAGCCAA
    ATTACAGGCT ACAGGATTCA GCTTCTTCCA ATGTTAGCGG GAAGTAAACA ACATTCACTG
    AACCTTGGCC CTCAAACTAC AAGTATAAAT GTAAAAGATC TCTCCCCTGA TACAGAATAC
    CAAATAAATT TATATGCCAT GAAAGGACTC ACAGCAAGTG AGCCAATCTC AACACTGGAA
    AAAACCCAGG CTGTGAAAAT TAAAGTCGAA TGTTCCGGTG GTGTCGATAT AAAAGCAGAT
    GTCGTTCTGC TTGTTGATGG CTCCTACAGT ATTGGTGTAG CTAATTTTGC CAAAGTTAGA
    GCCTTCCTTG AAGTATTAGT AAAAAGCTTT GATATTTCGC CCAGCAAAGT ACAGATCAGT
    CTTGTGCAAT ACAGTAGAGA TCCATTTACA GAGTTTACCT TAAACAGGTA TGACTCAATT
    GAAGACATTC TCAAAGCTGT AAATACCTTT CCATATAGAG GTGGCTCTAC CAACACTGGC
    AAAGCAATGA CCTACGTGAG AGAAAAGGTC TTTGTTGAAA TTAAAGGAGC AAGACCAAAC
    GTCCCTAGAG TCATGATTCT TATCACCGAT GGAAAGTCAT CAGATGCCTT TAAAGATCCA
    GCTATAAAAC TGCGGAATTC AGATGTTGAG ATTTTTGCAG TTGGTGTGAA GGATGCAGTG
    AGATCTGAGC TGGAGGCCAT TGCTTCTCCT CCTTCAGACA CCCATGTGTA CACAGTGGAA
    GATTTTGATG CTTTTCAAAG GATTTCTTTT GAGCTAACAC AGTCTATCTG CCTTAGAATT
    GAGCAAGAGC TACAAACAAT AAAACGTAAA TCTTTGACCC CTGCAAGAGA TTTGGCTTTC
    TCTGAGGTTA CATCCAACAG TTTTCGTGTG AACTGGTCTC CTGCGGCTGA GGATGTTTTG
    TCATACCTTG TTAAATATAA GGTGGCTGTT GGAGAAGGTG AAGAATTCAT CGTTTCCGTG
    CCAGCACCAA AAACAACCAC AGTTCTTTCA AATTTACTTC CAGAAACTAC TTATGCTGTG
    GCTGTCCTGG CAGAATACGA GGAAGGAGAG AGTCCTCCAT TAAATGGGGA AGAAACAACT
    TTAGAAGTTA CTGGCCCACC AAGAAACCTG AGAGTAAGTG ACGAAACAAC TGATAGCTTT
    AAAGTTGGCT GGTCACCAGC TCCTGGCAAT GTACTGAGGT ACAGAATTGC CTACCGGCCT
    GTGGCAGGAG GGGCAAGAAG AGAGGTTACA GTCCCAGGAA GGGAGACCGT GACAACACTC
    CAGAACCTCA CACCTGATAC AAAGTACCAA GTATCTGTGA TTCCTGAATA TCATTCGGGT
    CCTGGAAAAC CTTTGACAGG AAATGGAGCC ACAGAAGAAG TGTTGGGAGA ACCAAGGAAT
    CTGAGGGTTT CTGATGCCAC AACCTCCAGT TTAAAACTGT CCTGGGATTC GGCACCAGGC
    AAAGTGTTAC AGTATCTTGT AACTTACAAC CCTTCTGCAG AAGGGGAAAC TAAAGAGGTT
    ACAGTAAGAG GAAATACAAC CAGTACAGTA CTGAAGGGAT TAGACCCAAG CACCACATAC
    TCCCTTTCTG TAGCTGCACT CTATAGATCT GGCGCTGGCT CATTACTTAC TGGGGAAGGA
    ACAACTCTTG ATGAACGTGG TTCTCCTCGT AACCTGGTAA CCAAAGACAT TACAGACACT
    ACAATTGGAG CATCATGGAC AGCAGCCCCA GGGATGGTTC GTGGTTACAG AATTGCATGG
    AAGTCTCTTT TTGATGATGA CTCTGGAGAA AAGAATGTTC CTGGTGGTAT TACTAACACT
    GTGCTGGAAA ACCTGAGGCC AGAAACAAAG TATAAAATTT CAGTGTTTGC TAATTATAGA
    AGTGGAGAAG GAGACCCTCT TGAAGGAGAA GCCACCACTG AAGCGTCACC AAGTGCCAAG
    CTGCTTAAAG TCAGTGATGA GACAGAAACT ACAATGAAGG TAACCTGGCA GCCTGCCCCA
    GGAAATGTTG CAAATTATCG GGTTATATAT CGGCCGAAGC TTGGAGGAAG GCAAGTGGTC
    GCTAAAGTGC CAGCTACAGT CTCCTCAACA GTACTGAAAA GGCTTCAACC TCAAACAACC
    TATGATATTA CAGTTGTTCC TGTTTATAAG GCAGGGGATG GAAAACCAAG ACAAGGAGAA
    GGGACAACAG CTTCTCCTTT CAAGCCCCCA AGGAATCTGA AAACATCTGA CCCCACAATC
    TCAACTTTCC GAGTCACCTG GGAACCTGCT CCTGGTGAAG TTAGGGGTTA TAAAATCACA
    TATTTTCCTT TTGGAGAGGA GCAATATTTG GGAGAGTTAG TAGTTGGACC ATATGATAAC
    ACTGTGGTAC TAGAAGAACT CAGGGCAGGA ACATCTTATA AAGTCAATGT ATTTGGAGTT
    TTTGAAAAAG GAGAGAGTGT ACCTTTAATT GGACAAGAAA TGACAACATT GTCAGATGCT
    CCTGAAGTTC GCATTGATTC GTCAGGCCTG GAATGCAAAA CTAAAGCTGC AGCTGATATA
    GTGTTGCTGG TGGATGGCTC TTGGAGTATC GGCCGCCCAA ATTTTAGGAC CGTCAGGAGT
    TTTATAGCAC GACTGGTTGA AGTCTTTGAT ATTGGCTCAG ACAGAGTGCA GATTGCCTTG
    GCCCAATACA GTGGGGACCC TCGATCTGAA TGGCAACTCA ATGCTCATAG CACAAAGAAG
    TCTTTAATGG ATGCCGTTGC AAACCTTCCT TACAAGGGGG GTAACACTCT TACAGGCATG
    GCTCTGAATT TCATCTTGCA AAATAACTTC AAGGCAGAGG CAGGCATGAG AGCAAAGTCA
    AAGAAAATTG GTGTCTTAAT CACTGATGGA AAGTCACAAG ATGACATTGT TGCACCTTCC
    AAAAAGTTAC GAGACCAGGG AATAGACTTA TTTGCAATTG GTATCAAAAA TGCTGATGAA
    AATGAACTGA GGCAGATTGG CTCAGATCCA GATGAGACTT ACGTATTTAA CGTTGCCGAT
    TTTTCTTTGC TTGTCAATAT TGTTGATGAC CTCACTACCA ATCTATGTAA CAGTGTGAAA
    GGCTCAGGAA ATGAACTGGA GCCACCTTCA GACCTAGTAA CATCAGAGCC TACACCACGG
    AGCTTCAGAG TCACATGGAA TCCACCTTCT CACAGTGTAG ACCGATTTAG GGTGGAATAT
    TATCCAGTGT CTGGTGGTCG ACCTCTGGAG GTATTTGTTG GCAGATCAGA TAGAACTACG
    GTTTTGACTA ATTTAAAGCC AGAAACAGAG TATGTTGTGA AAGTGTATTC TGTGTTAGAA
    GGAGAAAGCA GTGAACCATT AGAAGGCACA GAAACGACAT TGCCCATCCC TTCAGTCAGA
    AATCTTAACA TATATGATAT AGGAACCAGA ACAATGAGAG TTCGATGGGA AGCTGCGCTT
    GGAGCTACTG GTTATTTATT GCAGTATGAG CCAGTAAACG CTACTGTGCC TTCAACTGAA
    AAAGAGTTGA GAGTTGGTGC ATCGGTGACA GATGTACTTC TGGAAGATTT ATTCCCCAAC
    ACTGAGTACA CCTTGACTGT ATATGCACTT TTTGATGAGC TGATCAGTGA TCCTCTTACC
    TCACAGGAAG TCACTTTACC CATTCAAGGC GTGGGGAACA TTAGAGTGAG AGATATCACT
    CACAGCTCAA TGAATGTCCT TTGGAGTGCT GCTCCTGGAA ATGTTCTCAA ATATATAGTA
    AGATATAAAA CAGAGGAAGA AGAAGAATTC AAAGAGGTGG AAGTGGATGG ATCAAGGACA
    AGTACACGAC TTACTGACCT TTTCTCACAA ACTCTATATG AAATCAGTTT AACTGCAGTA
    TATGATGAAG GAGTATCTCA GCCACGTACT ACACAAGGAA CTACAGCACC AGTACCAGCT
    CCAGCAAACC TCCGGTTTTC TGAAATAGCA GTAACAAGTT TCCGAGGTAC TTGGGAGCAT
    GGGGCTCCAG ATGTAGCTTT GTACAGGATA ACGTGGGCAC CAGTTGGAGC CATAGAAAAA
    AAGAAAGAGA CAATTGTTGA TGGTGATATA AACACTTTGG TTTTTGATGA ACTGGAACCA
    GACACAGAGT ATGAGGTTTC AGTAACTGCG ATTTACCCTG ACGAATCAGA GAGTGAAGAT
    CTGATCGGTG TTGAAAGGAC ATTGCCTGAA ATAATTTCTA CAACACCTGC CCTAAGGACT
    GGCCCTAGGA ATCTACAGGT GTTCAATGCA ACACAGAATA GTTTGACTGT ACGATGGGAT
    CGTGCTATGG GCGATGTGCA AAAATACAAT ATCTATTATA AGCCTGTTTC TGCTGCTGGT
    GCTGAACAAA TGACAAGTGT TGGTGGAAAG CAGAACAGTG TTGTTCTTCA GCGACTGCAG
    CCAGACACTC CATACAACAT TGTTGTTTCA TCAAGGTACA TTGGAGATGA GGAAGGAGGA
    AATATCTCAG GGAGAGGAAA AACAAAGCCC TTAAATACAG TGAAGAATCT AAGAGTGTAT
    GACCCAACAA CAAGCACTTT AAATGTACGT TGGGAAGCTG CAGAAGGAAA TGTGCGGCGG
    TATAAAATTT ACTATGTACC AACTGCTGGA GGACCAGAGG AAACGGTGAC AGTGCCCGGA
    AATACAATCT ATTCAGTTCT TAGGAATCTT CAGTCTGATA CATCATATAC AGTAACTGTT
    GTTCCTGTCT ATTCTGAAGG AGAAGGAGGT CGTATGTCAG AAACAGGAAA GACTTTGGTG
    CGGGGAACAG TTCGAAATAT TCAAGTATAC AACCCCACAA CAAACACTTT GAATGTTCGC
    TGGGAGCCTG CGCCTGGACA GGTCCAGCAA TATAAAGTCA ACTATGCTCC ACTTCTTGGA
    ACAAGGCCTA CTGAGTCTGT TGTAGTTCCT GGCAATACTC ACAACGCGCT GCTGGAGCAA
    CTTATTCCTG ACACACAGTA CTCTGTAAAT GTTGTGGCTC TTTATGCAGA TGGTGAGGGG
    GATCCTAGCC CTGGTGAAGG AAAAACATTA CCTCGCAGTG GGCCAAAAAA TATTAGAGTT
    TTTGGAGAGA CCACTAACAG TCTCTCTGTG AACTGGGATC ATGCTGATGG ACCAGTCCAA
    CAATACAGAA TCATCTATTC ACCAACTGTT GGAGACCCCA TTGATGAATA TACAACAGTG
    CCTGGTAGGA GAAATAATGT GGTACTGCAG CCCCTTTTGT CTGACACGCC ATATAAAATT
    ACTGTGGTGG CAGTTTATGC TGATGGTGAT GGAGGACAAC TGACAGGAAA AGGAAGGACA
    GTGGGTTTGA TTGCACCACG GAACCTTCAA GTGTCTGATG AATGGTACAC AAGATTTAGG
    GTTTCCTGGG ATAGAATACC ATCTCCTGTT CTTGGATACA AGCTGGTTTA TCAGCCACGT
    GATTCAGATG AATCAATGGA AGTATTTGTC GGGGATGTGA GCTCCTACAC TTTGCAGAAC
    CTGTCTCCTG GCACCACATA TGATGTGAAA GTTTACGCTC AGTATGATAC AGGATTAAGT
    GGGCCCCTAT CAGGACCTGG GACAACTTTA TACTTAAATG TAACCGATTT GACAAGCTAT
    CAGGTTTCAT GGGACTCGTT CTGTATAAGA TGGACTCCTC ACCGATCAGC CACTTCCTAC
    AGACTGAAAC TGAATCCAGT TGATGGATCT CGAGGTCAAG AAATAACAGT AACAGGATCA
    GAATCATCAC ACTGTTTTAA TGGACTGAAC CAAGATACAC AGTACACAGC TACTGTTTTT
    GTGCAAACAC CTAACCTTGA AGGACGAGGA GTTTCAATAG ATGAGCGTAC ATTGATCAAG
    CCTACAGAAT CACCAACGAC ACCACCTGCT CCTCCTCCAC CCCCTACAAT TCCACCTGCT
    CGGGAGGTGT GCAAAGGGGC CAAAGCAGAC ATAGTATTCC TCATTGACAG TTCGTGGAGT
    ATTGGTGATG ATAACTACAA TAAAGTCCGT CAGTTTATCT TCAATACTAT TGGAGCATTC
    GATGTAATCA ACCCTTCTGG AATCCAGGTC TCTTTTGTGC AGTTCAGCGA TGATGCCCAG
    ACGGAGTTCA AGTTGAACAC TTATGCCGAC AAATCACAGG CTCTAGGAGC ACTTGCCAAT
    ATCAGATACA AAGGAGGAAA CACAAAAACA GGAAAGGCTC TTAAATTTGT CCACGAGAAA
    GTGATGACAG TTGAGAATGG AATGAGAAGA TCTGTTCCTA GGGTTCTGGT AGTGGTGACT
    GATGGCAGAT CTCAGGATGA TGTTAAGAAA TCAGCCTTGG ACATTCAGCA ATCTGGTTTT
    AGTGTCTTTG TCATTGGTGT GGCTGATGTG GATTATAATG AGCTAAGAAA CATTGGCAGT
    AAACCAAGCG AGCGCCATGT TTTTATAGTC GATGACTTTG ATGCTTTTGA GAAGATTGAA
    GATAACCTGA TCAACTTTGT ATGTGAGACT GCAACATCCA CTTGCCCACT TATTTACCTG
    GAAGGATACA CTTCACCAGG TTTCAGAATG CTGGAAGCAT ATAACCTGAC AGAGAAAACT
    TTTGCCTCAG TACAAGGTGT CTCAATGCAA CCAGGATCTT TCAACAGTTT CCCTGCATAC
    AACCTACATA AAGATGCCTT CATTTCCCTG CCTACCTCAG AATTGCACCC AGAAGGATTA
    CCACCTTCAT ATACGATTAT ATTGCTATTC CGTCTGCTGC CAGAAACTCC CAATGAGCCA
    TTTTCTCTAT GGCAAATAAC AGATAAAGGT TACAAGCCAC AAGTAGGAGT AAATCTTGAT
    CCATCTAAGA AAACACTGTC GTTCTTTAAT AAGGGAGCTG CTGGGGATAC TCAGACAGTA
    ACATTTGATG GGAATGAAGT GAAGAAACTC TTTTATGGAA GCTTCCACAA GGTTCTCATT
    GTGGTTTCTC CATCATACGT CAGAATTTAT ATTGATTGTA GTGAAGTTGC TGAAAAAGAA
    ATCAAAGAAG CTGGCAATAT TACAGCAGAA GGATATGAAG TACTTGGGAA GACATTAAAG
    GGTGATAAAA AATCTGCACC CTTCCTGCTC CAGATGTTTG ACATTGTGTG TACTGTGACC
    TGGACAAGTC GAGATAGATG CTGTGATATT CCTGCTACAA GGGATGAAGC CAAATGTCCA
    GCTCTGCCCC ATTCCTGTAC TTGCGCACAA GACAGCGTTG GAGCACCAGG ACCCCCAGGC
    CCATCCGGTA TTCCCGGTAG TAAAGGACCA CGGGGTGACC GAGGTTTGGA TGGACCAGTT
    GGCCCACCAG GTCAGCGAGG AGAACAAGGT TTGCCTGGTC CACAAGGACC ACCTGGACCT
    CAAGGACCAC CTGGACTTTC CATTCCTGGA GAACCTGGTC GTCAAGGAAT AAAAGGAGAT
    GCCGGTGACC CAGGATTGCC AGGAAGAATG GGTTCCCCAG GGGTGACAGG ACCACCAGGG
    CCTATGGGTC CACCAGGCAC AAGGGGTTTC ACAGGAAAGG ATGGTGCAGT AGGACCCAGA
    GGACCACCAG GACCAATGGG AGCACCTGGA CCTCAGGGAG TGCCAGGTCC AAGTGGGAAA
    CCAGGAAAGC AAGGAGATAA TGGATTGCCA GGTCCTCTTG GACAGAAAGG AGACAAGGGT
    GAGAGGGGAG ACATGGCATC TCAGAATATG ATGAGATCAG TAGCAAGACA AGTCTGTGAA
    CAGATGATAA ATAGTCAAAT GAGCAGATTT AATCAGATGT TGAACCAAAT TCCAAATGAT
    TACTATTCCA ATCGCAATCA GCCTGGTCCT CCAGGTCCTC CCGGTCCTCC AGGCAACGCT
    GGCATGAGAG GAGAACCTGG AGCTGCTGGC AGATCTGGAT TCCCAGGAAC ACCTGGTCCT
    CAAGGACCAC CTGGAGAAAG AGGAATACCA GGAGAGAAAG GGGAGAGAGG GAACCCAGGA
    ATTGGATCCC AAGGACCCAG GGGACTTCCA GGACCTCCAG GACCTCCAGG CGAATCCAGA
    ACAGGACCTC CAGGATCCTC AGGCTCCACA GGACCTCCAG GACCTTCAGG TCGCCCCGGT
    AACCAAGGAA TTAGAGGGCC TCCTGGTCCA GCTGGATACT GTGATGCTTC AATGTGTGCA
    AGCATTCCTT ACAATGGGCA AGGATACCCA GAACCTTATC AGCCTGAAAG TGGACCGCAG
    GAGCCAGAGA ATCAGCCTTA CGTAGTACCT ATGCAGCAAG AGAGTCCAGA TGAGGATTTT
    GAGGACTATG GACATGAATA TGCAGAACAT ATGCGATGGA GGAGATCCTT ACCAAAGAAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002936246.3
    CDS186..9551
    Translation

    Target ORF information:

    RefSeq Version XM_002936200.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XII, alpha 1 (col12a1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002936200.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGTTGGTCC ACCTACAGAC TTAAATTTTG AAATAGTAAA TGAAAATACA
    GTTCGAATGT CATGGCAAAG GCCGGCAGAA AGAATAAAGG GTTACAAGAT TACTATCGTC
    CCTACAATTG ATGGACCAGA AAAGGAACTA GATCTTCCAG CTTCTGCTAC GCAAACCATA
    CTAACTGAAC TTGTACCAGA TGTGGAATAT GTTGTGACAG CTATTTCATA TGATGAATTA
    GAGGAGAGTG TACCTGTGTT TGGCCAACTT ACAATTCAAA CAGGAGGTCG AGTGACACTT
    GAAGAGCCTA AACTTGAGAC AAGTAAATCA CCACGCTGCT CTATTAGTTC TGTTGCTGAT
    GTAGTTTTCC TTGTGGATGG ATCATGGAGT GTTGGAAGAA ACAACTTCAA ATATATTCTC
    GATTTCATGG TTTCTCTGGT CTCAGCCTTT GATATTGGGG AAGACAAAAC CCGAGTCGGT
    GTTGTGCAGT ATAGCTCTGA CACTAGGACT GAGTTTAACC TGAACAGTTA TTACAAGAAA
    GAGGAATTAG TATCAGCCAT AAAAAGAATA CCATACAAGG GAGGCAATAC AATGACAGGT
    GATGCCCTTG ATTACCTGAT TAAGAACTCC TTTGTCAAAA CTGCCGGCTC AAGAAAAGGA
    TTTCCAAGAA TAGCTATCAT TATAACTGAT GGTAAATCCC AAGATGAAGT GGAGATTCCA
    GCAAGAGAGC TGCGTAGCTT AGGGGTTGAA GTTTTTTCCC TTGGTATAAA AGCAGCAGAT
    GCAAAAGAAC TCAAACAAAT AGCTTCTCTG CCTTCCTTGA AACATGTTTT TAATGTTGCT
    AATTTTGATT CGATTGTGGA AGTGCAAGAT GAAATAATTA CTCAAGTTTG CTCAGGAGTT
    GAAGAGCAGC TAAGTGAGCT TGTTAGTGGA GAAGAAGCTG TTGAGCCTCC TTCAGATATG
    ACTGTTACGG AAATTTCTTC CAAATCAATG AAGATCACCT GGAGACCATC GCCAAGCCAA
    ATTACAGGCT ACAGGATTCA GCTTCTTCCA ATGTTAGCGG GAAGTAAACA ACATTCACTG
    AACCTTGGCC CTCAAACTAC AAGTATAAAT GTAAAAGATC TCTCCCCTGA TACAGAATAC
    CAAATAAATT TATATGCCAT GAAAGGACTC ACAGCAAGTG AGCCAATCTC AACACTGGAA
    AAAACCCAGG CTGTGAAAAT TAAAGTCGAA TGTTCCGGTG GTGTCGATAT AAAAGCAGAT
    GTCGTTCTGC TTGTTGATGG CTCCTACAGT ATTGGTGTAG CTAATTTTGC CAAAGTTAGA
    GCCTTCCTTG AAGTATTAGT AAAAAGCTTT GATATTTCGC CCAGCAAAGT ACAGATCAGT
    CTTGTGCAAT ACAGTAGAGA TCCATTTACA GAGTTTACCT TAAACAGGTA TGACTCAATT
    GAAGACATTC TCAAAGCTGT AAATACCTTT CCATATAGAG GTGGCTCTAC CAACACTGGC
    AAAGCAATGA CCTACGTGAG AGAAAAGGTC TTTGTTGAAA TTAAAGGAGC AAGACCAAAC
    GTCCCTAGAG TCATGATTCT TATCACCGAT GGAAAGTCAT CAGATGCCTT TAAAGATCCA
    GCTATAAAAC TGCGGAATTC AGATGTTGAG ATTTTTGCAG TTGGTGTGAA GGATGCAGTG
    AGATCTGAGC TGGAGGCCAT TGCTTCTCCT CCTTCAGACA CCCATGTGTA CACAGTGGAA
    GATTTTGATG CTTTTCAAAG GATTTCTTTT GAGCTAACAC AGTCTATCTG CCTTAGAATT
    GAGCAAGAGC TACAAACAAT AAAACGTAAA TCTTTGACCC CTGCAAGAGA TTTGGCTTTC
    TCTGAGGTTA CATCCAACAG TTTTCGTGTG AACTGGTCTC CTGCGGCTGA GGATGTTTTG
    TCATACCTTG TTAAATATAA GGTGGCTGTT GGAGAAGGTG AAGAATTCAT CGTTTCCGTG
    CCAGCACCAA AAACAACCAC AGTTCTTTCA AATTTACTTC CAGAAACTAC TTATGCTGTG
    GCTGTCCTGG CAGAATACGA GGAAGGAGAG AGTCCTCCAT TAAATGGGGA AGAAACAACT
    TTAGAAGTTA CTGGCCCACC AAGAAACCTG AGAGTAAGTG ACGAAACAAC TGATAGCTTT
    AAAGTTGGCT GGTCACCAGC TCCTGGCAAT GTACTGAGGT ACAGAATTGC CTACCGGCCT
    GTGGCAGGAG GGGCAAGAAG AGAGGTTACA GTCCCAGGAA GGGAGACCGT GACAACACTC
    CAGAACCTCA CACCTGATAC AAAGTACCAA GTATCTGTGA TTCCTGAATA TCATTCGGGT
    CCTGGAAAAC CTTTGACAGG AAATGGAGCC ACAGAAGAAG TGTTGGGAGA ACCAAGGAAT
    CTGAGGGTTT CTGATGCCAC AACCTCCAGT TTAAAACTGT CCTGGGATTC GGCACCAGGC
    AAAGTGTTAC AGTATCTTGT AACTTACAAC CCTTCTGCAG AAGGGGAAAC TAAAGAGGTT
    ACAGTAAGAG GAAATACAAC CAGTACAGTA CTGAAGGGAT TAGACCCAAG CACCACATAC
    TCCCTTTCTG TAGCTGCACT CTATAGATCT GGCGCTGGCT CATTACTTAC TGGGGAAGGA
    ACAACTCTTG ATGAACGTGG TTCTCCTCGT AACCTGGTAA CCAAAGACAT TACAGACACT
    ACAATTGGAG CATCATGGAC AGCAGCCCCA GGGATGGTTC GTGGTTACAG AATTGCATGG
    AAGTCTCTTT TTGATGATGA CTCTGGAGAA AAGAATGTTC CTGGTGGTAT TACTAACACT
    GTGCTGGAAA ACCTGAGGCC AGAAACAAAG TATAAAATTT CAGTGTTTGC TAATTATAGA
    AGTGGAGAAG GAGACCCTCT TGAAGGAGAA GCCACCACTG AAGCGTCACC AAGTGCCAAG
    CTGCTTAAAG TCAGTGATGA GACAGAAACT ACAATGAAGG TAACCTGGCA GCCTGCCCCA
    GGAAATGTTG CAAATTATCG GGTTATATAT CGGCCGAAGC TTGGAGGAAG GCAAGTGGTC
    GCTAAAGTGC CAGCTACAGT CTCCTCAACA GTACTGAAAA GGCTTCAACC TCAAACAACC
    TATGATATTA CAGTTGTTCC TGTTTATAAG GCAGGGGATG GAAAACCAAG ACAAGGAGAA
    GGGACAACAG CTTCTCCTTT CAAGCCCCCA AGGAATCTGA AAACATCTGA CCCCACAATC
    TCAACTTTCC GAGTCACCTG GGAACCTGCT CCTGGTGAAG TTAGGGGTTA TAAAATCACA
    TATTTTCCTT TTGGAGAGGA GCAATATTTG GGAGAGTTAG TAGTTGGACC ATATGATAAC
    ACTGTGGTAC TAGAAGAACT CAGGGCAGGA ACATCTTATA AAGTCAATGT ATTTGGAGTT
    TTTGAAAAAG GAGAGAGTGT ACCTTTAATT GGACAAGAAA TGACAACATT GTCAGATGCT
    CCTGAAGTTC GCATTGATTC GTCAGGCCTG GAATGCAAAA CTAAAGCTGC AGCTGATATA
    GTGTTGCTGG TGGATGGCTC TTGGAGTATC GGCCGCCCAA ATTTTAGGAC CGTCAGGAGT
    TTTATAGCAC GACTGGTTGA AGTCTTTGAT ATTGGCTCAG ACAGAGTGCA GATTGCCTTG
    GCCCAATACA GTGGGGACCC TCGATCTGAA TGGCAACTCA ATGCTCATAG CACAAAGAAG
    TCTTTAATGG ATGCCGTTGC AAACCTTCCT TACAAGGGGG GTAACACTCT TACAGGCATG
    GCTCTGAATT TCATCTTGCA AAATAACTTC AAGGCAGAGG CAGGCATGAG AGCAAAGTCA
    AAGAAAATTG GTGTCTTAAT CACTGATGGA AAGTCACAAG ATGACATTGT TGCACCTTCC
    AAAAAGTTAC GAGACCAGGG AATAGACTTA TTTGCAATTG GTATCAAAAA TGCTGATGAA
    AATGAACTGA GGCAGATTGG CTCAGATCCA GATGAGACTT ACGTATTTAA CGTTGCCGAT
    TTTTCTTTGC TTGTCAATAT TGTTGATGAC CTCACTACCA ATCTATGTAA CAGTGTGAAA
    GGCTCAGGAA ATGAACTGGA GCCACCTTCA GACCTAGTAA CATCAGAGCC TACACCACGG
    AGCTTCAGAG TCACATGGAA TCCACCTTCT CACAGTGTAG ACCGATTTAG GGTGGAATAT
    TATCCAGTGT CTGGTGGTCG ACCTCTGGAG GTATTTGTTG GCAGATCAGA TAGAACTACG
    GTTTTGACTA ATTTAAAGCC AGAAACAGAG TATGTTGTGA AAGTGTATTC TGTGTTAGAA
    GGAGAAAGCA GTGAACCATT AGAAGGCACA GAAACGACAT TGCCCATCCC TTCAGTCAGA
    AATCTTAACA TATATGATAT AGGAACCAGA ACAATGAGAG TTCGATGGGA AGCTGCGCTT
    GGAGCTACTG GTTATTTATT GCAGTATGAG CCAGTAAACG CTACTGTGCC TTCAACTGAA
    AAAGAGTTGA GAGTTGGTGC ATCGGTGACA GATGTACTTC TGGAAGATTT ATTCCCCAAC
    ACTGAGTACA CCTTGACTGT ATATGCACTT TTTGATGAGC TGATCAGTGA TCCTCTTACC
    TCACAGGAAG TCACTTTACC CATTCAAGGC GTGGGGAACA TTAGAGTGAG AGATATCACT
    CACAGCTCAA TGAATGTCCT TTGGAGTGCT GCTCCTGGAA ATGTTCTCAA ATATATAGTA
    AGATATAAAA CAGAGGAAGA AGAAGAATTC AAAGAGGTGG AAGTGGATGG ATCAAGGACA
    AGTACACGAC TTACTGACCT TTTCTCACAA ACTCTATATG AAATCAGTTT AACTGCAGTA
    TATGATGAAG GAGTATCTCA GCCACGTACT ACACAAGGAA CTACAGCACC AGTACCAGCT
    CCAGCAAACC TCCGGTTTTC TGAAATAGCA GTAACAAGTT TCCGAGGTAC TTGGGAGCAT
    GGGGCTCCAG ATGTAGCTTT GTACAGGATA ACGTGGGCAC CAGTTGGAGC CATAGAAAAA
    AAGAAAGAGA CAATTGTTGA TGGTGATATA AACACTTTGG TTTTTGATGA ACTGGAACCA
    GACACAGAGT ATGAGGTTTC AGTAACTGCG ATTTACCCTG ACGAATCAGA GAGTGAAGAT
    CTGATCGGTG TTGAAAGGAC ATTGCCTGAA ATAATTTCTA CAACACCTGC CCTAAGGACT
    GGCCCTAGGA ATCTACAGGT GTTCAATGCA ACACAGAATA GTTTGACTGT ACGATGGGAT
    CGTGCTATGG GCGATGTGCA AAAATACAAT ATCTATTATA AGCCTGTTTC TGCTGCTGGT
    GCTGAACAAA TGACAAGTGT TGGTGGAAAG CAGAACAGTG TTGTTCTTCA GCGACTGCAG
    CCAGACACTC CATACAACAT TGTTGTTTCA TCAAGGTACA TTGGAGATGA GGAAGGAGGA
    AATATCTCAG GGAGAGGAAA AACAAAGCCC TTAAATACAG TGAAGAATCT AAGAGTGTAT
    GACCCAACAA CAAGCACTTT AAATGTACGT TGGGAAGCTG CAGAAGGAAA TGTGCGGCGG
    TATAAAATTT ACTATGTACC AACTGCTGGA GGACCAGAGG AAACGGTGAC AGTGCCCGGA
    AATACAATCT ATTCAGTTCT TAGGAATCTT CAGTCTGATA CATCATATAC AGTAACTGTT
    GTTCCTGTCT ATTCTGAAGG AGAAGGAGGT CGTATGTCAG AAACAGGAAA GACTTTGGTG
    CGGGGAACAG TTCGAAATAT TCAAGTATAC AACCCCACAA CAAACACTTT GAATGTTCGC
    TGGGAGCCTG CGCCTGGACA GGTCCAGCAA TATAAAGTCA ACTATGCTCC ACTTCTTGGA
    ACAAGGCCTA CTGAGTCTGT TGTAGTTCCT GGCAATACTC ACAACGCGCT GCTGGAGCAA
    CTTATTCCTG ACACACAGTA CTCTGTAAAT GTTGTGGCTC TTTATGCAGA TGGTGAGGGG
    GATCCTAGCC CTGGTGAAGG AAAAACATTA CCTCGCAGTG GGCCAAAAAA TATTAGAGTT
    TTTGGAGAGA CCACTAACAG TCTCTCTGTG AACTGGGATC ATGCTGATGG ACCAGTCCAA
    CAATACAGAA TCATCTATTC ACCAACTGTT GGAGACCCCA TTGATGAATA TACAACAGTG
    CCTGGTAGGA GAAATAATGT GGTACTGCAG CCCCTTTTGT CTGACACGCC ATATAAAATT
    ACTGTGGTGG CAGTTTATGC TGATGGTGAT GGAGGACAAC TGACAGGAAA AGGAAGGACA
    GTGGGTTTGA TTGCACCACG GAACCTTCAA GTGTCTGATG AATGGTACAC AAGATTTAGG
    GTTTCCTGGG ATAGAATACC ATCTCCTGTT CTTGGATACA AGCTGGTTTA TCAGCCACGT
    GATTCAGATG AATCAATGGA AGTATTTGTC GGGGATGTGA GCTCCTACAC TTTGCAGAAC
    CTGTCTCCTG GCACCACATA TGATGTGAAA GTTTACGCTC AGTATGATAC AGGATTAAGT
    GGGCCCCTAT CAGGACCTGG GACAACTTTA TACTTAAATG TAACCGATTT GACAAGCTAT
    CAGGTTTCAT GGGACTCGTT CTGTATAAGA TGGACTCCTC ACCGATCAGC CACTTCCTAC
    AGACTGAAAC TGAATCCAGT TGATGGATCT CGAGGTCAAG AAATAACAGT AACAGGATCA
    GAATCATCAC ACTGTTTTAA TGGACTGAAC CAAGATACAC AGTACACAGC TACTGTTTTT
    GTGCAAACAC CTAACCTTGA AGGACGAGGA GTTTCAATAG ATGAGCGTAC ATTGATCAAG
    CCTACAGAAT CACCAACGAC ACCACCTGCT CCTCCTCCAC CCCCTACAAT TCCACCTGCT
    CGGGAGGTGT GCAAAGGGGC CAAAGCAGAC ATAGTATTCC TCATTGACAG TTCGTGGAGT
    ATTGGTGATG ATAACTACAA TAAAGTCCGT CAGTTTATCT TCAATACTAT TGGAGCATTC
    GATGTAATCA ACCCTTCTGG AATCCAGGTC TCTTTTGTGC AGTTCAGCGA TGATGCCCAG
    ACGGAGTTCA AGTTGAACAC TTATGCCGAC AAATCACAGG CTCTAGGAGC ACTTGCCAAT
    ATCAGATACA AAGGAGGAAA CACAAAAACA GGAAAGGCTC TTAAATTTGT CCACGAGAAA
    GTGATGACAG TTGAGAATGG AATGAGAAGA TCTGTTCCTA GGGTTCTGGT AGTGGTGACT
    GATGGCAGAT CTCAGGATGA TGTTAAGAAA TCAGCCTTGG ACATTCAGCA ATCTGGTTTT
    AGTGTCTTTG TCATTGGTGT GGCTGATGTG GATTATAATG AGCTAAGAAA CATTGGCAGT
    AAACCAAGCG AGCGCCATGT TTTTATAGTC GATGACTTTG ATGCTTTTGA GAAGATTGAA
    GATAACCTGA TCAACTTTGT ATGTGAGACT GCAACATCCA CTTGCCCACT TATTTACCTG
    GAAGGATACA CTTCACCAGG TTTCAGAATG CTGGAAGCAT ATAACCTGAC AGAGAAAACT
    TTTGCCTCAG TACAAGGTGT CTCAATGCAA CCAGGATCTT TCAACAGTTT CCCTGCATAC
    AACCTACATA AAGATGCCTT CATTTCCCTG CCTACCTCAG AATTGCACCC AGAAGGATTA
    CCACCTTCAT ATACGATTAT ATTGCTATTC CGTCTGCTGC CAGAAACTCC CAATGAGCCA
    TTTTCTCTAT GGCAAATAAC AGATAAAGGT TACAAGCCAC AAGTAGGAGT AAATCTTGAT
    CCATCTAAGA AAACACTGTC GTTCTTTAAT AAGGGAGCTG CTGGGGATAC TCAGACAGTA
    ACATTTGATG GGAATGAAGT GAAGAAACTC TTTTATGGAA GCTTCCACAA GGTTCTCATT
    GTGGTTTCTC CATCATACGT CAGAATTTAT ATTGATTGTA GTGAAGTTGC TGAAAAAGAA
    ATCAAAGAAG CTGGCAATAT TACAGCAGAA GGATATGAAG TACTTGGGAA GACATTAAAG
    GGTGATAAAA AATCTGCACC CTTCCTGCTC CAGATGTTTG ACATTGTGTG TACTGTGACC
    TGGACAAGTC GAGATAGATG CTGTGATATT CCTGCTACAA GGGATGAAGC CAAATGTCCA
    GCTCTGCCCC ATTCCTGTAC TTGCGCACAA GACAGCGTTG GAGCACCAGG ACCCCCAGGC
    CCATCCGGTA TTCCCGGTAG TAAAGGACCA CGGGGTGACC GAGGTTTGGA TGGACCAGTT
    GGCCCACCAG GTCAGCGAGG AGAACAAGGT TTGCCTGGTC CACAAGGACC ACCTGGACCT
    CAAGGACCAC CTGGACTTTC CATTCCTGGA GAACCTGGTC GTCAAGGAAT AAAAGGAGAT
    GCCGGTGACC CAGGATTGCC AGGAAGAATG GGTTCCCCAG GGGTGACAGG ACCACCAGGG
    CCTATGGGTC CACCAGGCAC AAGGGGTTTC ACAGGAAAGG ATGGTGCAGT AGGACCCAGA
    GGACCACCAG GACCAATGGG AGCACCTGGA CCTCAGGGAG TGCCAGGTCC AAGTGGGAAA
    CCAGGAAAGC AAGGAGATAA TGGATTGCCA GGTCCTCTTG GACAGAAAGG AGACAAGGGT
    GAGAGGGGAG ACATGGCATC TCAGAATATG ATGAGATCAG TAGCAAGACA AGTCTGTGAA
    CAGATGATAA ATAGTCAAAT GAGCAGATTT AATCAGATGT TGAACCAAAT TCCAAATGAT
    TACTATTCCA ATCGCAATCA GCCTGGTCCT CCAGGTCCTC CCGGTCCTCC AGGCAACGCT
    GGCATGAGAG GAGAACCTGG AGCTGCTGGC AGATCTGGAT TCCCAGGAAC ACCTGGTCCT
    CAAGGACCAC CTGGAGAAAG AGGAATACCA GGAGAGAAAG GGGAGAGAGG GAACCCAGGA
    ATTGGATCCC AAGGACCCAG GGGACTTCCA GGACCTCCAG GACCTCCAGG CGAATCCAGA
    ACAGGACCTC CAGGATCCTC AGGCTCCACA GGACCTCCAG GACCTTCAGG TCGCCCCGGT
    AACCAAGGAA TTAGAGGGCC TCCTGGTCCA GCTGGATACT GTGATGCTTC AATGTGTGCA
    AGCATTCCTT ACAATGGGCA AGGATACCCA GAACCTTATC AGCCTGAAAG TGGACCGCAG
    GAGCCAGAGA ATCAGCCTTA CGTAGTACCT ATGCAGCAAG AGAGTCCAGA TGAGGATTTT
    GAGGACTATG GACATGAATA TGCAGAACAT ATGCGATGGA GGAGATCCTT ACCAAAGAAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58275
    Clone ID Related Accession (Same CDS sequence) XM_002936200.3 , XM_031901995.1
    Accession Version XM_031901995.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XII) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    9301
    9361
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGTTGGTCC ACCTACAGAC TTAAATTTTG AAATAGTAAA TGAAAATACA
    GTTCGAATGT CATGGCAAAG GCCGGCAGAA AGAATAAAGG GTTACAAGAT TACTATCGTC
    CCTACAATTG ATGGACCAGA AAAGGAACTA GATCTTCCAG CTTCTGCTAC GCAAACCATA
    CTAACTGAAC TTGTACCAGA TGTGGAATAT GTTGTGACAG CTATTTCATA TGATGAATTA
    GAGGAGAGTG TACCTGTGTT TGGCCAACTT ACAATTCAAA CAGGAGGTCG AGTGACACTT
    GAAGAGCCTA AACTTGAGAC AAGTAAATCA CCACGCTGCT CTATTAGTTC TGTTGCTGAT
    GTAGTTTTCC TTGTGGATGG ATCATGGAGT GTTGGAAGAA ACAACTTCAA ATATATTCTC
    GATTTCATGG TTTCTCTGGT CTCAGCCTTT GATATTGGGG AAGACAAAAC CCGAGTCGGT
    GTTGTGCAGT ATAGCTCTGA CACTAGGACT GAGTTTAACC TGAACAGTTA TTACAAGAAA
    GAGGAATTAG TATCAGCCAT AAAAAGAATA CCATACAAGG GAGGCAATAC AATGACAGGT
    GATGCCCTTG ATTACCTGAT TAAGAACTCC TTTGTCAAAA CTGCCGGCTC AAGAAAAGGA
    TTTCCAAGAA TAGCTATCAT TATAACTGAT GGTAAATCCC AAGATGAAGT GGAGATTCCA
    GCAAGAGAGC TGCGTAGCTT AGGGGTTGAA GTTTTTTCCC TTGGTATAAA AGCAGCAGAT
    GCAAAAGAAC TCAAACAAAT AGCTTCTCTG CCTTCCTTGA AACATGTTTT TAATGTTGCT
    AATTTTGATT CGATTGTGGA AGTGCAAGAT GAAATAATTA CTCAAGTTTG CTCAGGAGTT
    GAAGAGCAGC TAAGTGAGCT TGTTAGTGGA GAAGAAGCTG TTGAGCCTCC TTCAGATATG
    ACTGTTACGG AAATTTCTTC CAAATCAATG AAGATCACCT GGAGACCATC GCCAAGCCAA
    ATTACAGGCT ACAGGATTCA GCTTCTTCCA ATGTTAGCGG GAAGTAAACA ACATTCACTG
    AACCTTGGCC CTCAAACTAC AAGTATAAAT GTAAAAGATC TCTCCCCTGA TACAGAATAC
    CAAATAAATT TATATGCCAT GAAAGGACTC ACAGCAAGTG AGCCAATCTC AACACTGGAA
    AAAACCCAGG CTGTGAAAAT TAAAGTCGAA TGTTCCGGTG GTGTCGATAT AAAAGCAGAT
    GTCGTTCTGC TTGTTGATGG CTCCTACAGT ATTGGTGTAG CTAATTTTGC CAAAGTTAGA
    GCCTTCCTTG AAGTATTAGT AAAAAGCTTT GATATTTCGC CCAGCAAAGT ACAGATCAGT
    CTTGTGCAAT ACAGTAGAGA TCCATTTACA GAGTTTACCT TAAACAGGTA TGACTCAATT
    GAAGACATTC TCAAAGCTGT AAATACCTTT CCATATAGAG GTGGCTCTAC CAACACTGGC
    AAAGCAATGA CCTACGTGAG AGAAAAGGTC TTTGTTGAAA TTAAAGGAGC AAGACCAAAC
    GTCCCTAGAG TCATGATTCT TATCACCGAT GGAAAGTCAT CAGATGCCTT TAAAGATCCA
    GCTATAAAAC TGCGGAATTC AGATGTTGAG ATTTTTGCAG TTGGTGTGAA GGATGCAGTG
    AGATCTGAGC TGGAGGCCAT TGCTTCTCCT CCTTCAGACA CCCATGTGTA CACAGTGGAA
    GATTTTGATG CTTTTCAAAG GATTTCTTTT GAGCTAACAC AGTCTATCTG CCTTAGAATT
    GAGCAAGAGC TACAAACAAT AAAACGTAAA TCTTTGACCC CTGCAAGAGA TTTGGCTTTC
    TCTGAGGTTA CATCCAACAG TTTTCGTGTG AACTGGTCTC CTGCGGCTGA GGATGTTTTG
    TCATACCTTG TTAAATATAA GGTGGCTGTT GGAGAAGGTG AAGAATTCAT CGTTTCCGTG
    CCAGCACCAA AAACAACCAC AGTTCTTTCA AATTTACTTC CAGAAACTAC TTATGCTGTG
    GCTGTCCTGG CAGAATACGA GGAAGGAGAG AGTCCTCCAT TAAATGGGGA AGAAACAACT
    TTAGAAGTTA CTGGCCCACC AAGAAACCTG AGAGTAAGTG ACGAAACAAC TGATAGCTTT
    AAAGTTGGCT GGTCACCAGC TCCTGGCAAT GTACTGAGGT ACAGAATTGC CTACCGGCCT
    GTGGCAGGAG GGGCAAGAAG AGAGGTTACA GTCCCAGGAA GGGAGACCGT GACAACACTC
    CAGAACCTCA CACCTGATAC AAAGTACCAA GTATCTGTGA TTCCTGAATA TCATTCGGGT
    CCTGGAAAAC CTTTGACAGG AAATGGAGCC ACAGAAGAAG TGTTGGGAGA ACCAAGGAAT
    CTGAGGGTTT CTGATGCCAC AACCTCCAGT TTAAAACTGT CCTGGGATTC GGCACCAGGC
    AAAGTGTTAC AGTATCTTGT AACTTACAAC CCTTCTGCAG AAGGGGAAAC TAAAGAGGTT
    ACAGTAAGAG GAAATACAAC CAGTACAGTA CTGAAGGGAT TAGACCCAAG CACCACATAC
    TCCCTTTCTG TAGCTGCACT CTATAGATCT GGCGCTGGCT CATTACTTAC TGGGGAAGGA
    ACAACTCTTG ATGAACGTGG TTCTCCTCGT AACCTGGTAA CCAAAGACAT TACAGACACT
    ACAATTGGAG CATCATGGAC AGCAGCCCCA GGGATGGTTC GTGGTTACAG AATTGCATGG
    AAGTCTCTTT TTGATGATGA CTCTGGAGAA AAGAATGTTC CTGGTGGTAT TACTAACACT
    GTGCTGGAAA ACCTGAGGCC AGAAACAAAG TATAAAATTT CAGTGTTTGC TAATTATAGA
    AGTGGAGAAG GAGACCCTCT TGAAGGAGAA GCCACCACTG AAGCGTCACC AAGTGCCAAG
    CTGCTTAAAG TCAGTGATGA GACAGAAACT ACAATGAAGG TAACCTGGCA GCCTGCCCCA
    GGAAATGTTG CAAATTATCG GGTTATATAT CGGCCGAAGC TTGGAGGAAG GCAAGTGGTC
    GCTAAAGTGC CAGCTACAGT CTCCTCAACA GTACTGAAAA GGCTTCAACC TCAAACAACC
    TATGATATTA CAGTTGTTCC TGTTTATAAG GCAGGGGATG GAAAACCAAG ACAAGGAGAA
    GGGACAACAG CTTCTCCTTT CAAGCCCCCA AGGAATCTGA AAACATCTGA CCCCACAATC
    TCAACTTTCC GAGTCACCTG GGAACCTGCT CCTGGTGAAG TTAGGGGTTA TAAAATCACA
    TATTTTCCTT TTGGAGAGGA GCAATATTTG GGAGAGTTAG TAGTTGGACC ATATGATAAC
    ACTGTGGTAC TAGAAGAACT CAGGGCAGGA ACATCTTATA AAGTCAATGT ATTTGGAGTT
    TTTGAAAAAG GAGAGAGTGT ACCTTTAATT GGACAAGAAA TGACAACATT GTCAGATGCT
    CCTGAAGTTC GCATTGATTC GTCAGGCCTG GAATGCAAAA CTAAAGCTGC AGCTGATATA
    GTGTTGCTGG TGGATGGCTC TTGGAGTATC GGCCGCCCAA ATTTTAGGAC CGTCAGGAGT
    TTTATAGCAC GACTGGTTGA AGTCTTTGAT ATTGGCTCAG ACAGAGTGCA GATTGCCTTG
    GCCCAATACA GTGGGGACCC TCGATCTGAA TGGCAACTCA ATGCTCATAG CACAAAGAAG
    TCTTTAATGG ATGCCGTTGC AAACCTTCCT TACAAGGGGG GTAACACTCT TACAGGCATG
    GCTCTGAATT TCATCTTGCA AAATAACTTC AAGGCAGAGG CAGGCATGAG AGCAAAGTCA
    AAGAAAATTG GTGTCTTAAT CACTGATGGA AAGTCACAAG ATGACATTGT TGCACCTTCC
    AAAAAGTTAC GAGACCAGGG AATAGACTTA TTTGCAATTG GTATCAAAAA TGCTGATGAA
    AATGAACTGA GGCAGATTGG CTCAGATCCA GATGAGACTT ACGTATTTAA CGTTGCCGAT
    TTTTCTTTGC TTGTCAATAT TGTTGATGAC CTCACTACCA ATCTATGTAA CAGTGTGAAA
    GGCTCAGGAA ATGAACTGGA GCCACCTTCA GACCTAGTAA CATCAGAGCC TACACCACGG
    AGCTTCAGAG TCACATGGAA TCCACCTTCT CACAGTGTAG ACCGATTTAG GGTGGAATAT
    TATCCAGTGT CTGGTGGTCG ACCTCTGGAG GTATTTGTTG GCAGATCAGA TAGAACTACG
    GTTTTGACTA ATTTAAAGCC AGAAACAGAG TATGTTGTGA AAGTGTATTC TGTGTTAGAA
    GGAGAAAGCA GTGAACCATT AGAAGGCACA GAAACGACAT TGCCCATCCC TTCAGTCAGA
    AATCTTAACA TATATGATAT AGGAACCAGA ACAATGAGAG TTCGATGGGA AGCTGCGCTT
    GGAGCTACTG GTTATTTATT GCAGTATGAG CCAGTAAACG CTACTGTGCC TTCAACTGAA
    AAAGAGTTGA GAGTTGGTGC ATCGGTGACA GATGTACTTC TGGAAGATTT ATTCCCCAAC
    ACTGAGTACA CCTTGACTGT ATATGCACTT TTTGATGAGC TGATCAGTGA TCCTCTTACC
    TCACAGGAAG TCACTTTACC CATTCAAGGC GTGGGGAACA TTAGAGTGAG AGATATCACT
    CACAGCTCAA TGAATGTCCT TTGGAGTGCT GCTCCTGGAA ATGTTCTCAA ATATATAGTA
    AGATATAAAA CAGAGGAAGA AGAAGAATTC AAAGAGGTGG AAGTGGATGG ATCAAGGACA
    AGTACACGAC TTACTGACCT TTTCTCACAA ACTCTATATG AAATCAGTTT AACTGCAGTA
    TATGATGAAG GAGTATCTCA GCCACGTACT ACACAAGGAA CTACAGCACC AGTACCAGCT
    CCAGCAAACC TCCGGTTTTC TGAAATAGCA GTAACAAGTT TCCGAGGTAC TTGGGAGCAT
    GGGGCTCCAG ATGTAGCTTT GTACAGGATA ACGTGGGCAC CAGTTGGAGC CATAGAAAAA
    AAGAAAGAGA CAATTGTTGA TGGTGATATA AACACTTTGG TTTTTGATGA ACTGGAACCA
    GACACAGAGT ATGAGGTTTC AGTAACTGCG ATTTACCCTG ACGAATCAGA GAGTGAAGAT
    CTGATCGGTG TTGAAAGGAC ATTGCCTGAA ATAATTTCTA CAACACCTGC CCTAAGGACT
    GGCCCTAGGA ATCTACAGGT GTTCAATGCA ACACAGAATA GTTTGACTGT ACGATGGGAT
    CGTGCTATGG GCGATGTGCA AAAATACAAT ATCTATTATA AGCCTGTTTC TGCTGCTGGT
    GCTGAACAAA TGACAAGTGT TGGTGGAAAG CAGAACAGTG TTGTTCTTCA GCGACTGCAG
    CCAGACACTC CATACAACAT TGTTGTTTCA TCAAGGTACA TTGGAGATGA GGAAGGAGGA
    AATATCTCAG GGAGAGGAAA AACAAAGCCC TTAAATACAG TGAAGAATCT AAGAGTGTAT
    GACCCAACAA CAAGCACTTT AAATGTACGT TGGGAAGCTG CAGAAGGAAA TGTGCGGCGG
    TATAAAATTT ACTATGTACC AACTGCTGGA GGACCAGAGG AAACGGTGAC AGTGCCCGGA
    AATACAATCT ATTCAGTTCT TAGGAATCTT CAGTCTGATA CATCATATAC AGTAACTGTT
    GTTCCTGTCT ATTCTGAAGG AGAAGGAGGT CGTATGTCAG AAACAGGAAA GACTTTGGTG
    CGGGGAACAG TTCGAAATAT TCAAGTATAC AACCCCACAA CAAACACTTT GAATGTTCGC
    TGGGAGCCTG CGCCTGGACA GGTCCAGCAA TATAAAGTCA ACTATGCTCC ACTTCTTGGA
    ACAAGGCCTA CTGAGTCTGT TGTAGTTCCT GGCAATACTC ACAACGCGCT GCTGGAGCAA
    CTTATTCCTG ACACACAGTA CTCTGTAAAT GTTGTGGCTC TTTATGCAGA TGGTGAGGGG
    GATCCTAGCC CTGGTGAAGG AAAAACATTA CCTCGCAGTG GGCCAAAAAA TATTAGAGTT
    TTTGGAGAGA CCACTAACAG TCTCTCTGTG AACTGGGATC ATGCTGATGG ACCAGTCCAA
    CAATACAGAA TCATCTATTC ACCAACTGTT GGAGACCCCA TTGATGAATA TACAACAGTG
    CCTGGTAGGA GAAATAATGT GGTACTGCAG CCCCTTTTGT CTGACACGCC ATATAAAATT
    ACTGTGGTGG CAGTTTATGC TGATGGTGAT GGAGGACAAC TGACAGGAAA AGGAAGGACA
    GTGGGTTTGA TTGCACCACG GAACCTTCAA GTGTCTGATG AATGGTACAC AAGATTTAGG
    GTTTCCTGGG ATAGAATACC ATCTCCTGTT CTTGGATACA AGCTGGTTTA TCAGCCACGT
    GATTCAGATG AATCAATGGA AGTATTTGTC GGGGATGTGA GCTCCTACAC TTTGCAGAAC
    CTGTCTCCTG GCACCACATA TGATGTGAAA GTTTACGCTC AGTATGATAC AGGATTAAGT
    GGGCCCCTAT CAGGACCTGG GACAACTTTA TACTTAAATG TAACCGATTT GACAAGCTAT
    CAGGTTTCAT GGGACTCGTT CTGTATAAGA TGGACTCCTC ACCGATCAGC CACTTCCTAC
    AGACTGAAAC TGAATCCAGT TGATGGATCT CGAGGTCAAG AAATAACAGT AACAGGATCA
    GAATCATCAC ACTGTTTTAA TGGACTGAAC CAAGATACAC AGTACACAGC TACTGTTTTT
    GTGCAAACAC CTAACCTTGA AGGACGAGGA GTTTCAATAG ATGAGCGTAC ATTGATCAAG
    CCTACAGAAT CACCAACGAC ACCACCTGCT CCTCCTCCAC CCCCTACAAT TCCACCTGCT
    CGGGAGGTGT GCAAAGGGGC CAAAGCAGAC ATAGTATTCC TCATTGACAG TTCGTGGAGT
    ATTGGTGATG ATAACTACAA TAAAGTCCGT CAGTTTATCT TCAATACTAT TGGAGCATTC
    GATGTAATCA ACCCTTCTGG AATCCAGGTC TCTTTTGTGC AGTTCAGCGA TGATGCCCAG
    ACGGAGTTCA AGTTGAACAC TTATGCCGAC AAATCACAGG CTCTAGGAGC ACTTGCCAAT
    ATCAGATACA AAGGAGGAAA CACAAAAACA GGAAAGGCTC TTAAATTTGT CCACGAGAAA
    GTGATGACAG TTGAGAATGG AATGAGAAGA TCTGTTCCTA GGGTTCTGGT AGTGGTGACT
    GATGGCAGAT CTCAGGATGA TGTTAAGAAA TCAGCCTTGG ACATTCAGCA ATCTGGTTTT
    AGTGTCTTTG TCATTGGTGT GGCTGATGTG GATTATAATG AGCTAAGAAA CATTGGCAGT
    AAACCAAGCG AGCGCCATGT TTTTATAGTC GATGACTTTG ATGCTTTTGA GAAGATTGAA
    GATAACCTGA TCAACTTTGT ATGTGAGACT GCAACATCCA CTTGCCCACT TATTTACCTG
    GAAGGATACA CTTCACCAGG TTTCAGAATG CTGGAAGCAT ATAACCTGAC AGAGAAAACT
    TTTGCCTCAG TACAAGGTGT CTCAATGCAA CCAGGATCTT TCAACAGTTT CCCTGCATAC
    AACCTACATA AAGATGCCTT CATTTCCCTG CCTACCTCAG AATTGCACCC AGAAGGATTA
    CCACCTTCAT ATACGATTAT ATTGCTATTC CGTCTGCTGC CAGAAACTCC CAATGAGCCA
    TTTTCTCTAT GGCAAATAAC AGATAAAGGT TACAAGCCAC AAGTAGGAGT AAATCTTGAT
    CCATCTAAGA AAACACTGTC GTTCTTTAAT AAGGGAGCTG CTGGGGATAC TCAGACAGTA
    ACATTTGATG GGAATGAAGT GAAGAAACTC TTTTATGGAA GCTTCCACAA GGTTCTCATT
    GTGGTTTCTC CATCATACGT CAGAATTTAT ATTGATTGTA GTGAAGTTGC TGAAAAAGAA
    ATCAAAGAAG CTGGCAATAT TACAGCAGAA GGATATGAAG TACTTGGGAA GACATTAAAG
    GGTGATAAAA AATCTGCACC CTTCCTGCTC CAGATGTTTG ACATTGTGTG TACTGTGACC
    TGGACAAGTC GAGATAGATG CTGTGATATT CCTGCTACAA GGGATGAAGC CAAATGTCCA
    GCTCTGCCCC ATTCCTGTAC TTGCGCACAA GACAGCGTTG GAGCACCAGG ACCCCCAGGC
    CCATCCGGTA TTCCCGGTAG TAAAGGACCA CGGGGTGACC GAGGTTTGGA TGGACCAGTT
    GGCCCACCAG GTCAGCGAGG AGAACAAGGT TTGCCTGGTC CACAAGGACC ACCTGGACCT
    CAAGGACCAC CTGGACTTTC CATTCCTGGA GAACCTGGTC GTCAAGGAAT AAAAGGAGAT
    GCCGGTGACC CAGGATTGCC AGGAAGAATG GGTTCCCCAG GGGTGACAGG ACCACCAGGG
    CCTATGGGTC CACCAGGCAC AAGGGGTTTC ACAGGAAAGG ATGGTGCAGT AGGACCCAGA
    GGACCACCAG GACCAATGGG AGCACCTGGA CCTCAGGGAG TGCCAGGTCC AAGTGGGAAA
    CCAGGAAAGC AAGGAGATAA TGGATTGCCA GGTCCTCTTG GACAGAAAGG AGACAAGGGT
    GAGAGGGGAG ACATGGCATC TCAGAATATG ATGAGATCAG TAGCAAGACA AGTCTGTGAA
    CAGATGATAA ATAGTCAAAT GAGCAGATTT AATCAGATGT TGAACCAAAT TCCAAATGAT
    TACTATTCCA ATCGCAATCA GCCTGGTCCT CCAGGTCCTC CCGGTCCTCC AGGCAACGCT
    GGCATGAGAG GAGAACCTGG AGCTGCTGGC AGATCTGGAT TCCCAGGAAC ACCTGGTCCT
    CAAGGACCAC CTGGAGAAAG AGGAATACCA GGAGAGAAAG GGGAGAGAGG GAACCCAGGA
    ATTGGATCCC AAGGACCCAG GGGACTTCCA GGACCTCCAG GACCTCCAGG CGAATCCAGA
    ACAGGACCTC CAGGATCCTC AGGCTCCACA GGACCTCCAG GACCTTCAGG TCGCCCCGGT
    AACCAAGGAA TTAGAGGGCC TCCTGGTCCA GCTGGATACT GTGATGCTTC AATGTGTGCA
    AGCATTCCTT ACAATGGGCA AGGATACCCA GAACCTTATC AGCCTGAAAG TGGACCGCAG
    GAGCCAGAGA ATCAGCCTTA CGTAGTACCT ATGCAGCAAG AGAGTCCAGA TGAGGATTTT
    GAGGACTATG GACATGAATA TGCAGAACAT ATGCGATGGA GGAGATCCTT ACCAAAGAAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757855.1
    CDS189..9554
    Translation

    Target ORF information:

    RefSeq Version XM_031901995.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XII, alpha 1 (col12a1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGTTGGTCC ACCTACAGAC TTAAATTTTG AAATAGTAAA TGAAAATACA
    GTTCGAATGT CATGGCAAAG GCCGGCAGAA AGAATAAAGG GTTACAAGAT TACTATCGTC
    CCTACAATTG ATGGACCAGA AAAGGAACTA GATCTTCCAG CTTCTGCTAC GCAAACCATA
    CTAACTGAAC TTGTACCAGA TGTGGAATAT GTTGTGACAG CTATTTCATA TGATGAATTA
    GAGGAGAGTG TACCTGTGTT TGGCCAACTT ACAATTCAAA CAGGAGGTCG AGTGACACTT
    GAAGAGCCTA AACTTGAGAC AAGTAAATCA CCACGCTGCT CTATTAGTTC TGTTGCTGAT
    GTAGTTTTCC TTGTGGATGG ATCATGGAGT GTTGGAAGAA ACAACTTCAA ATATATTCTC
    GATTTCATGG TTTCTCTGGT CTCAGCCTTT GATATTGGGG AAGACAAAAC CCGAGTCGGT
    GTTGTGCAGT ATAGCTCTGA CACTAGGACT GAGTTTAACC TGAACAGTTA TTACAAGAAA
    GAGGAATTAG TATCAGCCAT AAAAAGAATA CCATACAAGG GAGGCAATAC AATGACAGGT
    GATGCCCTTG ATTACCTGAT TAAGAACTCC TTTGTCAAAA CTGCCGGCTC AAGAAAAGGA
    TTTCCAAGAA TAGCTATCAT TATAACTGAT GGTAAATCCC AAGATGAAGT GGAGATTCCA
    GCAAGAGAGC TGCGTAGCTT AGGGGTTGAA GTTTTTTCCC TTGGTATAAA AGCAGCAGAT
    GCAAAAGAAC TCAAACAAAT AGCTTCTCTG CCTTCCTTGA AACATGTTTT TAATGTTGCT
    AATTTTGATT CGATTGTGGA AGTGCAAGAT GAAATAATTA CTCAAGTTTG CTCAGGAGTT
    GAAGAGCAGC TAAGTGAGCT TGTTAGTGGA GAAGAAGCTG TTGAGCCTCC TTCAGATATG
    ACTGTTACGG AAATTTCTTC CAAATCAATG AAGATCACCT GGAGACCATC GCCAAGCCAA
    ATTACAGGCT ACAGGATTCA GCTTCTTCCA ATGTTAGCGG GAAGTAAACA ACATTCACTG
    AACCTTGGCC CTCAAACTAC AAGTATAAAT GTAAAAGATC TCTCCCCTGA TACAGAATAC
    CAAATAAATT TATATGCCAT GAAAGGACTC ACAGCAAGTG AGCCAATCTC AACACTGGAA
    AAAACCCAGG CTGTGAAAAT TAAAGTCGAA TGTTCCGGTG GTGTCGATAT AAAAGCAGAT
    GTCGTTCTGC TTGTTGATGG CTCCTACAGT ATTGGTGTAG CTAATTTTGC CAAAGTTAGA
    GCCTTCCTTG AAGTATTAGT AAAAAGCTTT GATATTTCGC CCAGCAAAGT ACAGATCAGT
    CTTGTGCAAT ACAGTAGAGA TCCATTTACA GAGTTTACCT TAAACAGGTA TGACTCAATT
    GAAGACATTC TCAAAGCTGT AAATACCTTT CCATATAGAG GTGGCTCTAC CAACACTGGC
    AAAGCAATGA CCTACGTGAG AGAAAAGGTC TTTGTTGAAA TTAAAGGAGC AAGACCAAAC
    GTCCCTAGAG TCATGATTCT TATCACCGAT GGAAAGTCAT CAGATGCCTT TAAAGATCCA
    GCTATAAAAC TGCGGAATTC AGATGTTGAG ATTTTTGCAG TTGGTGTGAA GGATGCAGTG
    AGATCTGAGC TGGAGGCCAT TGCTTCTCCT CCTTCAGACA CCCATGTGTA CACAGTGGAA
    GATTTTGATG CTTTTCAAAG GATTTCTTTT GAGCTAACAC AGTCTATCTG CCTTAGAATT
    GAGCAAGAGC TACAAACAAT AAAACGTAAA TCTTTGACCC CTGCAAGAGA TTTGGCTTTC
    TCTGAGGTTA CATCCAACAG TTTTCGTGTG AACTGGTCTC CTGCGGCTGA GGATGTTTTG
    TCATACCTTG TTAAATATAA GGTGGCTGTT GGAGAAGGTG AAGAATTCAT CGTTTCCGTG
    CCAGCACCAA AAACAACCAC AGTTCTTTCA AATTTACTTC CAGAAACTAC TTATGCTGTG
    GCTGTCCTGG CAGAATACGA GGAAGGAGAG AGTCCTCCAT TAAATGGGGA AGAAACAACT
    TTAGAAGTTA CTGGCCCACC AAGAAACCTG AGAGTAAGTG ACGAAACAAC TGATAGCTTT
    AAAGTTGGCT GGTCACCAGC TCCTGGCAAT GTACTGAGGT ACAGAATTGC CTACCGGCCT
    GTGGCAGGAG GGGCAAGAAG AGAGGTTACA GTCCCAGGAA GGGAGACCGT GACAACACTC
    CAGAACCTCA CACCTGATAC AAAGTACCAA GTATCTGTGA TTCCTGAATA TCATTCGGGT
    CCTGGAAAAC CTTTGACAGG AAATGGAGCC ACAGAAGAAG TGTTGGGAGA ACCAAGGAAT
    CTGAGGGTTT CTGATGCCAC AACCTCCAGT TTAAAACTGT CCTGGGATTC GGCACCAGGC
    AAAGTGTTAC AGTATCTTGT AACTTACAAC CCTTCTGCAG AAGGGGAAAC TAAAGAGGTT
    ACAGTAAGAG GAAATACAAC CAGTACAGTA CTGAAGGGAT TAGACCCAAG CACCACATAC
    TCCCTTTCTG TAGCTGCACT CTATAGATCT GGCGCTGGCT CATTACTTAC TGGGGAAGGA
    ACAACTCTTG ATGAACGTGG TTCTCCTCGT AACCTGGTAA CCAAAGACAT TACAGACACT
    ACAATTGGAG CATCATGGAC AGCAGCCCCA GGGATGGTTC GTGGTTACAG AATTGCATGG
    AAGTCTCTTT TTGATGATGA CTCTGGAGAA AAGAATGTTC CTGGTGGTAT TACTAACACT
    GTGCTGGAAA ACCTGAGGCC AGAAACAAAG TATAAAATTT CAGTGTTTGC TAATTATAGA
    AGTGGAGAAG GAGACCCTCT TGAAGGAGAA GCCACCACTG AAGCGTCACC AAGTGCCAAG
    CTGCTTAAAG TCAGTGATGA GACAGAAACT ACAATGAAGG TAACCTGGCA GCCTGCCCCA
    GGAAATGTTG CAAATTATCG GGTTATATAT CGGCCGAAGC TTGGAGGAAG GCAAGTGGTC
    GCTAAAGTGC CAGCTACAGT CTCCTCAACA GTACTGAAAA GGCTTCAACC TCAAACAACC
    TATGATATTA CAGTTGTTCC TGTTTATAAG GCAGGGGATG GAAAACCAAG ACAAGGAGAA
    GGGACAACAG CTTCTCCTTT CAAGCCCCCA AGGAATCTGA AAACATCTGA CCCCACAATC
    TCAACTTTCC GAGTCACCTG GGAACCTGCT CCTGGTGAAG TTAGGGGTTA TAAAATCACA
    TATTTTCCTT TTGGAGAGGA GCAATATTTG GGAGAGTTAG TAGTTGGACC ATATGATAAC
    ACTGTGGTAC TAGAAGAACT CAGGGCAGGA ACATCTTATA AAGTCAATGT ATTTGGAGTT
    TTTGAAAAAG GAGAGAGTGT ACCTTTAATT GGACAAGAAA TGACAACATT GTCAGATGCT
    CCTGAAGTTC GCATTGATTC GTCAGGCCTG GAATGCAAAA CTAAAGCTGC AGCTGATATA
    GTGTTGCTGG TGGATGGCTC TTGGAGTATC GGCCGCCCAA ATTTTAGGAC CGTCAGGAGT
    TTTATAGCAC GACTGGTTGA AGTCTTTGAT ATTGGCTCAG ACAGAGTGCA GATTGCCTTG
    GCCCAATACA GTGGGGACCC TCGATCTGAA TGGCAACTCA ATGCTCATAG CACAAAGAAG
    TCTTTAATGG ATGCCGTTGC AAACCTTCCT TACAAGGGGG GTAACACTCT TACAGGCATG
    GCTCTGAATT TCATCTTGCA AAATAACTTC AAGGCAGAGG CAGGCATGAG AGCAAAGTCA
    AAGAAAATTG GTGTCTTAAT CACTGATGGA AAGTCACAAG ATGACATTGT TGCACCTTCC
    AAAAAGTTAC GAGACCAGGG AATAGACTTA TTTGCAATTG GTATCAAAAA TGCTGATGAA
    AATGAACTGA GGCAGATTGG CTCAGATCCA GATGAGACTT ACGTATTTAA CGTTGCCGAT
    TTTTCTTTGC TTGTCAATAT TGTTGATGAC CTCACTACCA ATCTATGTAA CAGTGTGAAA
    GGCTCAGGAA ATGAACTGGA GCCACCTTCA GACCTAGTAA CATCAGAGCC TACACCACGG
    AGCTTCAGAG TCACATGGAA TCCACCTTCT CACAGTGTAG ACCGATTTAG GGTGGAATAT
    TATCCAGTGT CTGGTGGTCG ACCTCTGGAG GTATTTGTTG GCAGATCAGA TAGAACTACG
    GTTTTGACTA ATTTAAAGCC AGAAACAGAG TATGTTGTGA AAGTGTATTC TGTGTTAGAA
    GGAGAAAGCA GTGAACCATT AGAAGGCACA GAAACGACAT TGCCCATCCC TTCAGTCAGA
    AATCTTAACA TATATGATAT AGGAACCAGA ACAATGAGAG TTCGATGGGA AGCTGCGCTT
    GGAGCTACTG GTTATTTATT GCAGTATGAG CCAGTAAACG CTACTGTGCC TTCAACTGAA
    AAAGAGTTGA GAGTTGGTGC ATCGGTGACA GATGTACTTC TGGAAGATTT ATTCCCCAAC
    ACTGAGTACA CCTTGACTGT ATATGCACTT TTTGATGAGC TGATCAGTGA TCCTCTTACC
    TCACAGGAAG TCACTTTACC CATTCAAGGC GTGGGGAACA TTAGAGTGAG AGATATCACT
    CACAGCTCAA TGAATGTCCT TTGGAGTGCT GCTCCTGGAA ATGTTCTCAA ATATATAGTA
    AGATATAAAA CAGAGGAAGA AGAAGAATTC AAAGAGGTGG AAGTGGATGG ATCAAGGACA
    AGTACACGAC TTACTGACCT TTTCTCACAA ACTCTATATG AAATCAGTTT AACTGCAGTA
    TATGATGAAG GAGTATCTCA GCCACGTACT ACACAAGGAA CTACAGCACC AGTACCAGCT
    CCAGCAAACC TCCGGTTTTC TGAAATAGCA GTAACAAGTT TCCGAGGTAC TTGGGAGCAT
    GGGGCTCCAG ATGTAGCTTT GTACAGGATA ACGTGGGCAC CAGTTGGAGC CATAGAAAAA
    AAGAAAGAGA CAATTGTTGA TGGTGATATA AACACTTTGG TTTTTGATGA ACTGGAACCA
    GACACAGAGT ATGAGGTTTC AGTAACTGCG ATTTACCCTG ACGAATCAGA GAGTGAAGAT
    CTGATCGGTG TTGAAAGGAC ATTGCCTGAA ATAATTTCTA CAACACCTGC CCTAAGGACT
    GGCCCTAGGA ATCTACAGGT GTTCAATGCA ACACAGAATA GTTTGACTGT ACGATGGGAT
    CGTGCTATGG GCGATGTGCA AAAATACAAT ATCTATTATA AGCCTGTTTC TGCTGCTGGT
    GCTGAACAAA TGACAAGTGT TGGTGGAAAG CAGAACAGTG TTGTTCTTCA GCGACTGCAG
    CCAGACACTC CATACAACAT TGTTGTTTCA TCAAGGTACA TTGGAGATGA GGAAGGAGGA
    AATATCTCAG GGAGAGGAAA AACAAAGCCC TTAAATACAG TGAAGAATCT AAGAGTGTAT
    GACCCAACAA CAAGCACTTT AAATGTACGT TGGGAAGCTG CAGAAGGAAA TGTGCGGCGG
    TATAAAATTT ACTATGTACC AACTGCTGGA GGACCAGAGG AAACGGTGAC AGTGCCCGGA
    AATACAATCT ATTCAGTTCT TAGGAATCTT CAGTCTGATA CATCATATAC AGTAACTGTT
    GTTCCTGTCT ATTCTGAAGG AGAAGGAGGT CGTATGTCAG AAACAGGAAA GACTTTGGTG
    CGGGGAACAG TTCGAAATAT TCAAGTATAC AACCCCACAA CAAACACTTT GAATGTTCGC
    TGGGAGCCTG CGCCTGGACA GGTCCAGCAA TATAAAGTCA ACTATGCTCC ACTTCTTGGA
    ACAAGGCCTA CTGAGTCTGT TGTAGTTCCT GGCAATACTC ACAACGCGCT GCTGGAGCAA
    CTTATTCCTG ACACACAGTA CTCTGTAAAT GTTGTGGCTC TTTATGCAGA TGGTGAGGGG
    GATCCTAGCC CTGGTGAAGG AAAAACATTA CCTCGCAGTG GGCCAAAAAA TATTAGAGTT
    TTTGGAGAGA CCACTAACAG TCTCTCTGTG AACTGGGATC ATGCTGATGG ACCAGTCCAA
    CAATACAGAA TCATCTATTC ACCAACTGTT GGAGACCCCA TTGATGAATA TACAACAGTG
    CCTGGTAGGA GAAATAATGT GGTACTGCAG CCCCTTTTGT CTGACACGCC ATATAAAATT
    ACTGTGGTGG CAGTTTATGC TGATGGTGAT GGAGGACAAC TGACAGGAAA AGGAAGGACA
    GTGGGTTTGA TTGCACCACG GAACCTTCAA GTGTCTGATG AATGGTACAC AAGATTTAGG
    GTTTCCTGGG ATAGAATACC ATCTCCTGTT CTTGGATACA AGCTGGTTTA TCAGCCACGT
    GATTCAGATG AATCAATGGA AGTATTTGTC GGGGATGTGA GCTCCTACAC TTTGCAGAAC
    CTGTCTCCTG GCACCACATA TGATGTGAAA GTTTACGCTC AGTATGATAC AGGATTAAGT
    GGGCCCCTAT CAGGACCTGG GACAACTTTA TACTTAAATG TAACCGATTT GACAAGCTAT
    CAGGTTTCAT GGGACTCGTT CTGTATAAGA TGGACTCCTC ACCGATCAGC CACTTCCTAC
    AGACTGAAAC TGAATCCAGT TGATGGATCT CGAGGTCAAG AAATAACAGT AACAGGATCA
    GAATCATCAC ACTGTTTTAA TGGACTGAAC CAAGATACAC AGTACACAGC TACTGTTTTT
    GTGCAAACAC CTAACCTTGA AGGACGAGGA GTTTCAATAG ATGAGCGTAC ATTGATCAAG
    CCTACAGAAT CACCAACGAC ACCACCTGCT CCTCCTCCAC CCCCTACAAT TCCACCTGCT
    CGGGAGGTGT GCAAAGGGGC CAAAGCAGAC ATAGTATTCC TCATTGACAG TTCGTGGAGT
    ATTGGTGATG ATAACTACAA TAAAGTCCGT CAGTTTATCT TCAATACTAT TGGAGCATTC
    GATGTAATCA ACCCTTCTGG AATCCAGGTC TCTTTTGTGC AGTTCAGCGA TGATGCCCAG
    ACGGAGTTCA AGTTGAACAC TTATGCCGAC AAATCACAGG CTCTAGGAGC ACTTGCCAAT
    ATCAGATACA AAGGAGGAAA CACAAAAACA GGAAAGGCTC TTAAATTTGT CCACGAGAAA
    GTGATGACAG TTGAGAATGG AATGAGAAGA TCTGTTCCTA GGGTTCTGGT AGTGGTGACT
    GATGGCAGAT CTCAGGATGA TGTTAAGAAA TCAGCCTTGG ACATTCAGCA ATCTGGTTTT
    AGTGTCTTTG TCATTGGTGT GGCTGATGTG GATTATAATG AGCTAAGAAA CATTGGCAGT
    AAACCAAGCG AGCGCCATGT TTTTATAGTC GATGACTTTG ATGCTTTTGA GAAGATTGAA
    GATAACCTGA TCAACTTTGT ATGTGAGACT GCAACATCCA CTTGCCCACT TATTTACCTG
    GAAGGATACA CTTCACCAGG TTTCAGAATG CTGGAAGCAT ATAACCTGAC AGAGAAAACT
    TTTGCCTCAG TACAAGGTGT CTCAATGCAA CCAGGATCTT TCAACAGTTT CCCTGCATAC
    AACCTACATA AAGATGCCTT CATTTCCCTG CCTACCTCAG AATTGCACCC AGAAGGATTA
    CCACCTTCAT ATACGATTAT ATTGCTATTC CGTCTGCTGC CAGAAACTCC CAATGAGCCA
    TTTTCTCTAT GGCAAATAAC AGATAAAGGT TACAAGCCAC AAGTAGGAGT AAATCTTGAT
    CCATCTAAGA AAACACTGTC GTTCTTTAAT AAGGGAGCTG CTGGGGATAC TCAGACAGTA
    ACATTTGATG GGAATGAAGT GAAGAAACTC TTTTATGGAA GCTTCCACAA GGTTCTCATT
    GTGGTTTCTC CATCATACGT CAGAATTTAT ATTGATTGTA GTGAAGTTGC TGAAAAAGAA
    ATCAAAGAAG CTGGCAATAT TACAGCAGAA GGATATGAAG TACTTGGGAA GACATTAAAG
    GGTGATAAAA AATCTGCACC CTTCCTGCTC CAGATGTTTG ACATTGTGTG TACTGTGACC
    TGGACAAGTC GAGATAGATG CTGTGATATT CCTGCTACAA GGGATGAAGC CAAATGTCCA
    GCTCTGCCCC ATTCCTGTAC TTGCGCACAA GACAGCGTTG GAGCACCAGG ACCCCCAGGC
    CCATCCGGTA TTCCCGGTAG TAAAGGACCA CGGGGTGACC GAGGTTTGGA TGGACCAGTT
    GGCCCACCAG GTCAGCGAGG AGAACAAGGT TTGCCTGGTC CACAAGGACC ACCTGGACCT
    CAAGGACCAC CTGGACTTTC CATTCCTGGA GAACCTGGTC GTCAAGGAAT AAAAGGAGAT
    GCCGGTGACC CAGGATTGCC AGGAAGAATG GGTTCCCCAG GGGTGACAGG ACCACCAGGG
    CCTATGGGTC CACCAGGCAC AAGGGGTTTC ACAGGAAAGG ATGGTGCAGT AGGACCCAGA
    GGACCACCAG GACCAATGGG AGCACCTGGA CCTCAGGGAG TGCCAGGTCC AAGTGGGAAA
    CCAGGAAAGC AAGGAGATAA TGGATTGCCA GGTCCTCTTG GACAGAAAGG AGACAAGGGT
    GAGAGGGGAG ACATGGCATC TCAGAATATG ATGAGATCAG TAGCAAGACA AGTCTGTGAA
    CAGATGATAA ATAGTCAAAT GAGCAGATTT AATCAGATGT TGAACCAAAT TCCAAATGAT
    TACTATTCCA ATCGCAATCA GCCTGGTCCT CCAGGTCCTC CCGGTCCTCC AGGCAACGCT
    GGCATGAGAG GAGAACCTGG AGCTGCTGGC AGATCTGGAT TCCCAGGAAC ACCTGGTCCT
    CAAGGACCAC CTGGAGAAAG AGGAATACCA GGAGAGAAAG GGGAGAGAGG GAACCCAGGA
    ATTGGATCCC AAGGACCCAG GGGACTTCCA GGACCTCCAG GACCTCCAGG CGAATCCAGA
    ACAGGACCTC CAGGATCCTC AGGCTCCACA GGACCTCCAG GACCTTCAGG TCGCCCCGGT
    AACCAAGGAA TTAGAGGGCC TCCTGGTCCA GCTGGATACT GTGATGCTTC AATGTGTGCA
    AGCATTCCTT ACAATGGGCA AGGATACCCA GAACCTTATC AGCCTGAAAG TGGACCGCAG
    GAGCCAGAGA ATCAGCCTTA CGTAGTACCT ATGCAGCAAG AGAGTCCAGA TGAGGATTTT
    GAGGACTATG GACATGAATA TGCAGAACAT ATGCGATGGA GGAGATCCTT ACCAAAGAAA
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58276
    Clone ID Related Accession (Same CDS sequence) XM_018094325.2
    Accession Version XM_018094325.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-1(XII) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGTTGGTCC ACCTACAGAC TTAAATTTTG AAATAGTAAA TGAAAATACA
    GTTCGAATGT CATGGCAAAG GCCGGCAGAA AGAATAAAGG GTTACAAGAT TACTATCGTC
    CCTACAATTG ATGGACCAGA AAAGGAACTA GATCTTCCAG CTTCTGCTAC GCAAACCATA
    CTAACTGAAC TTGTACCAGA TGTGGAATAT GTTGTGACAG CTATTTCATA TGATGAATTA
    GAGGAGAGTG TACCTGTGTT TGGCCAACTT ACAATTCAAA CAGGAGGTCG AGTGACACTT
    GAAGAGCCTA AACTTGAGAC AAGTAAATCA CCACGCTGCT CTATTAGTTC TGTTGCTGAT
    GTAGTTTTCC TTGTGGATGG ATCATGGAGT GTTGGAAGAA ACAACTTCAA ATATATTCTC
    GATTTCATGG TTTCTCTGGT CTCAGCCTTT GATATTGGGG AAGACAAAAC CCGAGTCGGT
    GTTGTGCAGT ATAGCTCTGA CACTAGGACT GAGTTTAACC TGAACAGTTA TTACAAGAAA
    GAGGAATTAG TATCAGCCAT AAAAAGAATA CCATACAAGG GAGGCAATAC AATGACAGGT
    GATGCCCTTG ATTACCTGAT TAAGAACTCC TTTGTCAAAA CTGCCGGCTC AAGAAAAGGA
    TTTCCAAGAA TAGCTATCAT TATAACTGAT GGTAAATCCC AAGATGAAGT GGAGATTCCA
    GCAAGAGAGC TGCGTAGCTT AGGGGTTGAA GTTTTTTCCC TTGGTATAAA AGCAGCAGAT
    GCAAAAGAAC TCAAACAAAT AGCTTCTCTG CCTTCCTTGA AACATGTTTT TAATGTTGCT
    AATTTTGATT CGATTGTGGA AGTGCAAGAT GAAATAATTA CTCAAGTTTG CTCAGGAGTT
    GAAGAGCAGC TAAGTGAGCT TGTTAGTGGA GAAGAAGCTG TTGAGCCTCC TTCAGATATG
    ACTGTTACGG AAATTTCTTC CAAATCAATG AAGATCACCT GGAGACCATC GCCAAGCCAA
    ATTACAGGCT ACAGGATTCA GCTTCTTCCA ATGTTAGCGG GAAGTAAACA ACATTCACTG
    AACCTTGGCC CTCAAACTAC AAGTATAAAT GTAAAAGATC TCTCCCCTGA TACAGAATAC
    CAAATAAATT TATATGCCAT GAAAGGACTC ACAGCAAGTG AGCCAATCTC AACACTGGAA
    AAAACCCAGG CTGTGAAAAT TAAAGTCGAA TGTTCCGGTG GTGTCGATAT AAAAGCAGAT
    GTCGTTCTGC TTGTTGATGG CTCCTACAGT ATTGGTGTAG CTAATTTTGC CAAAGTTAGA
    GCCTTCCTTG AAGTATTAGT AAAAAGCTTT GATATTTCGC CCAGCAAAGT ACAGATCAGT
    CTTGTGCAAT ACAGTAGAGA TCCATTTACA GAGTTTACCT TAAACAGGTA TGACTCAATT
    GAAGACATTC TCAAAGCTGT AAATACCTTT CCATATAGAG GTGGCTCTAC CAACACTGGC
    AAAGCAATGA CCTACGTGAG AGAAAAGGTC TTTGTTGAAA TTAAAGGAGC AAGACCAAAC
    GTCCCTAGAG TCATGATTCT TATCACCGAT GGAAAGTCAT CAGATGCCTT TAAAGATCCA
    GCTATAAAAC TGCGGAATTC AGATGTTGAG ATTTTTGCAG TTGGTGTGAA GGATGCAGTG
    AGATCTGAGC TGGAGGCCAT TGCTTCTCCT CCTTCAGACA CCCATGTGTA CACAGTGGAA
    GATTTTGATG CTTTTCAAAG GATTTCTTTT GAGCTAACAC AGTCTATCTG CCTTAGAATT
    GAGCAAGAGC TACAAACAAT AAAACGTAAA TCTTTGACCC CTGCAAGAGA TTTGGCTTTC
    TCTGAGGTTA CATCCAACAG TTTTCGTGTG AACTGGTCTC CTGCGGCTGA GGATGTTTTG
    TCATACCTTG TTAAATATAA GGTGGCTGTT GGAGAAGGTG AAGAATTCAT CGTTTCCGTG
    CCAGCACCAA AAACAACCAC AGTTCTTTCA AATTTACTTC CAGAAACTAC TTATGCTGTG
    GCTGTCCTGG CAGAATACGA GGAAGGAGAG AGTCCTCCAT TAAATGGGGA AGAAACAACT
    TTAGAAGTTA CTGGCCCACC AAGAAACCTG AGAGTAAGTG ACGAAACAAC TGATAGCTTT
    AAAGTTGGCT GGTCACCAGC TCCTGGCAAT GTACTGAGGT ACAGAATTGC CTACCGGCCT
    GTGGCAGGAG GGGCAAGAAG AGAGGTTACA GTCCCAGGAA GGGAGACCGT GACAACACTC
    CAGAACCTCA CACCTGATAC AAAGTACCAA GTATCTGTGA TTCCTGAATA TCATTCGGGT
    CCTGGAAAAC CTTTGACAGG AAATGGAGCC ACAGAAGAAG TGTTGGGAGA ACCAAGGAAT
    CTGAGGGTTT CTGATGCCAC AACCTCCAGT TTAAAACTGT CCTGGGATTC GGCACCAGGC
    AAAGTGTTAC AGTATCTTGT AACTTACAAC CCTTCTGCAG AAGGGGAAAC TAAAGAGGTT
    ACAGTAAGAG GAAATACAAC CAGTACAGTA CTGAAGGGAT TAGACCCAAG CACCACATAC
    TCCCTTTCTG TAGCTGCACT CTATAGATCT GGCGCTGGCT CATTACTTAC TGGGGAAGGA
    ACAACTCTTG ATGAACGTGG TTCTCCTCGT AACCTGGTAA CCAAAGACAT TACAGACACT
    ACAATTGGAG CATCATGGAC AGCAGCCCCA GGGATGGTTC GTGGTTACAG AATTGCATGG
    AAGTCTCTTT TTGATGATGA CTCTGGAGAA AAGAATGTTC CTGGTGGTAT TACTAACACT
    GTGCTGGAAA ACCTGAGGCC AGAAACAAAG TATAAAATTT CAGTGTTTGC TAATTATAGA
    AGTGGAGAAG GAGACCCTCT TGAAGGAGAA GCCACCACTG AAGCGTCACC AAGTGCCAAG
    CTGCTTAAAG TCAGTGATGA GACAGAAACT ACAATGAAGG TAACCTGGCA GCCTGCCCCA
    GGAAATGTTG CAAATTATCG GGTTATATAT CGGCCGAAGC TTGGAGGAAG GCAAGTGGTC
    GCTAAAGTGC CAGCTACAGT CTCCTCAACA GTACTGAAAA GGCTTCAACC TCAAACAACC
    TATGATATTA CAGTTGTTCC TGTTTATAAG GCAGGGGATG GAAAACCAAG ACAAGGAGAA
    GGGACAACAG CTTCTCCTTT CAAGCCCCCA AGGAATCTGA AAACATCTGA CCCCACAATC
    TCAACTTTCC GAGTCACCTG GGAACCTGCT CCTGGTGAAG TTAGGGGTTA TAAAATCACA
    TATTTTCCTT TTGGAGAGGA GCAATATTTG GGAGAGTTAG TAGTTGGACC ATATGATAAC
    ACTGTGGTAC TAGAAGAACT CAGGGCAGGA ACATCTTATA AAGTCAATGT ATTTGGAGTT
    TTTGAAAAAG GAGAGAGTGT ACCTTTAATT GGACAAGAAA TGACAACATT GTCAGATGCT
    CCTGAAGTTC GCATTGATTC GTCAGGCCTG GAATGCAAAA CTAAAGCTGC AGCTGATATA
    GTGTTGCTGG TGGATGGCTC TTGGAGTATC GGCCGCCCAA ATTTTAGGAC CGTCAGGAGT
    TTTATAGCAC GACTGGTTGA AGTCTTTGAT ATTGGCTCAG ACAGAGTGCA GATTGCCTTG
    GCCCAATACA GTGGGGACCC TCGATCTGAA TGGCAACTCA ATGCTCATAG CACAAAGAAG
    TCTTTAATGG ATGCCGTTGC AAACCTTCCT TACAAGGGGG GTAACACTCT TACAGGCATG
    GCTCTGAATT TCATCTTGCA AAATAACTTC AAGGCAGAGG CAGGCATGAG AGCAAAGTCA
    AAGAAAATTG GTGTCTTAAT CACTGATGGA AAGTCACAAG ATGACATTGT TGCACCTTCC
    AAAAAGTTAC GAGACCAGGG AATAGACTTA TTTGCAATTG GTATCAAAAA TGCTGATGAA
    AATGAACTGA GGCAGATTGG CTCAGATCCA GATGAGACTT ACGTATTTAA CGTTGCCGAT
    TTTTCTTTGC TTGTCAATAT TGTTGATGAC CTCACTACCA ATCTATGTAA CAGTGTGAAA
    GGCTCAGGAA ATGAACTGGA GCCACCTTCA GACCTAGTAA CATCAGAGCC TACACCACGG
    AGCTTCAGAG TCACATGGAA TCCACCTTCT CACAGTGTAG ACCGATTTAG GGTGGAATAT
    TATCCAGTGT CTGGTGGTCG ACCTCTGGAG GTATTTGTTG GCAGATCAGA TAGAACTACG
    GTTTTGACTA ATTTAAAGCC AGAAACAGAG TATGTTGTGA AAGTGTATTC TGTGTTAGAA
    GGAGAAAGCA GTGAACCATT AGAAGGCACA GAAACGACAT TGCCCATCCC TTCAGTCAGA
    AATCTTAACA TATATGATAT AGGAACCAGA ACAATGAGAG TTCGATGGGA AGCTGCGCTT
    GGAGCTACTG GTTATTTATT GCAGTATGAG CCAGTAAACG CTACTGTGCC TTCAACTGAA
    AAAGAGTTGA GAGTTGGTGC ATCGGTGACA GATGTACTTC TGGAAGATTT ATTCCCCAAC
    ACTGAGTACA CCTTGACTGT ATATGCACTT TTTGATGAGC TGATCAGTGA TCCTCTTACC
    TCACAGGAAG TCACTTTACC CATTCAAGGC GTGGGGAACA TTAGAGTGAG AGATATCACT
    CACAGCTCAA TGAATGTCCT TTGGAGTGCT GCTCCTGGAA ATGTTCTCAA ATATATAGTA
    AGATATAAAA CAGAGGAAGA AGAAGAATTC AAAGAGGTGG AAGTGGATGG ATCAAGGACA
    AGTACACGAC TTACTGACCT TTTCTCACAA ACTCTATATG AAATCAGTTT AACTGCAGTA
    TATGATGAAG GAGTATCTCA GCCACGTACT ACACAAGGAA CTACAGCACC AGTACCAGCT
    CCAGCAAACC TCCGGTTTTC TGAAATAGCA GTAACAAGTT TCCGAGGTAC TTGGGAGCAT
    GGGGCTCCAG ATGTAGCTTT GTACAGGATA ACGTGGGCAC CAGTTGGAGC CATAGAAAAA
    AAGAAAGAGA CAATTGTTGA TGGTGATATA AACACTTTGG TTTTTGATGA ACTGGAACCA
    GACACAGAGT ATGAGGTTTC AGTAACTGCG ATTTACCCTG ACGAATCAGA GAGTGAAGAT
    CTGATCGGTG TTGAAAGGAC ATTGCCTGAA ATAATTTCTA CAACACCTGC CCTAAGGACT
    GGCCCTAGGA ATCTACAGGT GTTCAATGCA ACACAGAATA GTTTGACTGT ACGATGGGAT
    CGTGCTATGG GCGATGTGCA AAAATACAAT ATCTATTATA AGCCTGTTTC TGCTGCTGGT
    GCTGAACAAA TGACAAGTGT TGGTGGAAAG CAGAACAGTG TTGTTCTTCA GCGACTGCAG
    CCAGACACTC CATACAACAT TGTTGTTTCA TCAAGGTACA TTGGAGATGA GGAAGGAGGA
    AATATCTCAG GGAGAGGAAA AACAAAGCCC TTAAATACAG TGAAGAATCT AAGAGTGTAT
    GACCCAACAA CAAGCACTTT AAATGTACGT TGGGAAGCTG CAGAAGGAAA TGTGCGGCGG
    TATAAAATTT ACTATGTACC AACTGCTGGA GGACCAGAGG AAACGGTGAC AGTGCCCGGA
    AATACAATCT ATTCAGTTCT TAGGAATCTT CAGTCTGATA CATCATATAC AGTAACTGTT
    GTTCCTGTCT ATTCTGAAGG AGAAGGAGGT CGTATGTCAG AAACAGGAAA GACTTTGGTG
    CGGGGAACAG TTCGAAATAT TCAAGTATAC AACCCCACAA CAAACACTTT GAATGTTCGC
    TGGGAGCCTG CGCCTGGACA GGTCCAGCAA TATAAAGTCA ACTATGCTCC ACTTCTTGGA
    ACAAGGCCTA CTGAGTCTGT TGTAGTTCCT GGCAATACTC ACAACGCGCT GCTGGAGCAA
    CTTATTCCTG ACACACAGTA CTCTGTAAAT GTTGTGGCTC TTTATGCAGA TGGTGAGGGG
    GATCCTAGCC CTGGTGAAGG AAAAACATTA CCTCGCAGTG GGCCAAAAAA TATTAGAGTT
    TTTGGAGAGA CCACTAACAG TCTCTCTGTG AACTGGGATC ATGCTGATGG ACCAGTCCAA
    CAATACAGAA TCATCTATTC ACCAACTGTT GGAGACCCCA TTGATGAATA TACAACAGTG
    CCTGGTAGGA GAAATAATGT GGTACTGCAG CCCCTTTTGT CTGACACGCC ATATAAAATT
    ACTGTGGTGG CAGTTTATGC TGATGGTGAT GGAGGACAAC TGACAGGAAA AGGAAGGACA
    GTGGGTTTGA TTGCACCACG GAACCTTCAA GTGTCTGATG AATGGTACAC AAGATTTAGG
    GTTTCCTGGG ATAGAATACC ATCTCCTGTT CTTGGATACA AGCTGGTTTA TCAGCCACGT
    GATTCAGATG AATCAATGGA AGTATTTGTC GGGGATGTGA GCTCCTACAC TTTGCAGAAC
    CTGTCTCCTG GCACCACATA TGATGTGAAA GTTTACGCTC AGTATGATAC AGGATTAAGT
    GGGCCCCTAT CAGGACCTGG GACAACTTTA TACTTAAATG TAACCGATTT GACAAGCTAT
    CAGGTTTCAT GGGACTCGTT CTGTATAAGA TGGACTCCTC ACCGATCAGC CACTTCCTAC
    AGACTGAAAC TGAATCCAGT TGATGGATCT CGAGGTCAAG AAATAACAGT AACAGGATCA
    GAATCATCAC ACTGTTTTAA TGGACTGAAC CAAGATACAC AGTACACAGC TACTGTTTTT
    GTGCAAACAC CTAACCTTGA AGGACGAGGA GTTTCAATAG ATGAGCGTAC ATTGATCAAG
    CCTACAGAAT CACCAACGAC ACCACCTGCT CCTCCTCCAC CCCCTACAAT TCCACCTGCT
    CGGGAGGTGT GCAAAGGGGC CAAAGCAGAC ATAGTATTCC TCATTGACAG TTCGTGGAGT
    ATTGGTGATG ATAACTACAA TAAAGTCCGT CAGTTTATCT TCAATACTAT TGGAGCATTC
    GATGTAATCA ACCCTTCTGG AATCCAGGTC TCTTTTGTGC AGTTCAGCGA TGATGCCCAG
    ACGGAGTTCA AGTTGAACAC TTATGCCGAC AAATCACAGG CTCTAGGAGC ACTTGCCAAT
    ATCAGATACA AAGGAGGAAA CACAAAAACA GGAAAGGCTC TTAAATTTGT CCACGAGAAA
    GTGATGACAG TTGAGAATGG AATGAGAAGA TCTGTTCCTA GGGTTCTGGT AGTGGTGACT
    GATGGCAGAT CTCAGGATGA TGTTAAGAAA TCAGCCTTGG ACATTCAGCA ATCTGGTTTT
    AGTGTCTTTG TCATTGGTGT GGCTGATGTG GATTATAATG AGCTAAGAAA CATTGGCAGT
    AAACCAAGCG AGCGCCATGT TTTTATAGTC GATGACTTTG ATGCTTTTGA GAAGATTGAA
    GATAACCTGA TCAACTTTGT ATGTGAGACT GCAACATCCA CTTGCCCACT TATTTACCTG
    GAAGGATACA CTTCACCAGG TTTCAGAATG CTGGAAGCAT ATAACCTGAC AGAGAAAACT
    TTTGCCTCAG TACAAGGTGT CTCAATGCAA CCAGGATCTT TCAACAGTTT CCCTGCATAC
    AACCTACATA AAGATGCCTT CATTTCCCTG CCTACCTCAG AATTGCACCC AGAAGGATTA
    CCACCTTCAT ATACGATTAT ATTGCTATTC CGTCTGCTGC CAGAAACTCC CAATGAGCCA
    TTTTCTCTAT GGCAAATAAC AGATAAAGGT TACAAGCCAC AAGTAGGAGT AAATCTTGAT
    CCATCTAAGA AAACACTGTC GTTCTTTAAT AAGGGAGCTG CTGGGGATAC TCAGACAGTA
    ACATTTGATG GGAATGAAGT GAAGAAACTC TTTTATGGAA GCTTCCACAA GGTTCTCATT
    GTGGTTTCTC CATCATACGT CAGAATTTAT ATTGATTGTA GTGAAGTTGC TGAAAAAGAA
    ATCAAAGAAG CTGGCAATAT TACAGCAGAA GGATATGAAG TACTTGGGAA GACATTAAAG
    GGTGATAAAA AATCTGCACC CTTCCTGCTC CAGATGTTTG ACATTGTGTG TACTGTGACC
    TGGACAAGTC GAGATAGATG CTGTGATATT CCTGCTACAA GGGATGAAGC CAAATGTCCA
    GCTCTGCCCC ATTCCTGTAC TTGCGCACAA GACAGCGTTG GAGCACCAGG ACCCCCAGGC
    CCATCCGGTA TTCCCGGTAG TAAAGGACCA CGGGGTGACC GAGGTTTGGA TGGACCAGTT
    GGCCCACCAG GTCAGCGAGG AGAACAAGGT TTGCCTGGTC CACAAGGACC ACCTGGACCT
    CAAGGACCAC CTGGACTTTC CATTCCTGGA GAACCTGGTC GTCAAGGAAT AAAAGGAGAT
    GCCGGTGACC CAGGATTGCC AGGAAGAATG GGTTCCCCAG GGGTGACAGG ACCACCAGGG
    CCTATGGGTC CACCAGGCAC AAGGGGTTTC ACAGGAAAGG ATGGTGCAGT AGGACCCAGA
    GGACCACCAG GACCAATGGG AGCACCTGGA CCTCAGGGAG TGCCAGGTCC AAGTGGGAAA
    CCAGGAAAGC AAGGAGATAA TGGATTGCCA GGTCCTCTTG GACAGAAAGG AGACAAGGGT
    GAGAGGGGAG ACATGGCATC TCAGAATATG ATGAGATCAG TAGCAAGACA AGTCTGTGAA
    CAGATGATAA ATAGTCAAAT GAGCAGATTT AATCAGATGT TGAACCAAAT TCCAAATGAT
    TACTATTCCA ATCGCAATCA GCCTGGTCCT CCAGGTCCTC CCGGTCCTCC AGGCAACGCT
    GGCATGAGAG GAGAACCTGG AGCTGCTGGC AGATCTGGAT TCCCAGGAAC ACCTGGTCCT
    CAAGGACCAC CTGGAGAAAG AGGAATACCA GGAGAGAAAG GGGAGAGAGG GAACCCAGGA
    ATTGGATCCC AAGGACCCAG GGGACTTCCA GGACCTCCAG GACCTCCAGG CGAATCCAGA
    ACAGGACCTC CAGGATCCTC AGGCTCCACA GGACCTCCAG GACCTTCAGG TCGCCCCGGT
    AACCAAGGAA TTAGAGGGCC TCCTGGTCCA GCTGGATACT GTGATGCTTC AATGTGTGCA
    AGCATTCCTT ACAATGGGCA AGGATACCCA GGTTCCGGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949814.2
    CDS189..9410
    Translation

    Target ORF information:

    RefSeq Version XM_018094325.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type XII, alpha 1 (col12a1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094325.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGAAAATTC GGCTGTGCTC AGCAGTGACA GCATTTTGTG CTGTGCTGCT TCTGTCTTCC 
    ATAGAGGCTG AAGTTGGTCC ACCTACAGAC TTAAATTTTG AAATAGTAAA TGAAAATACA
    GTTCGAATGT CATGGCAAAG GCCGGCAGAA AGAATAAAGG GTTACAAGAT TACTATCGTC
    CCTACAATTG ATGGACCAGA AAAGGAACTA GATCTTCCAG CTTCTGCTAC GCAAACCATA
    CTAACTGAAC TTGTACCAGA TGTGGAATAT GTTGTGACAG CTATTTCATA TGATGAATTA
    GAGGAGAGTG TACCTGTGTT TGGCCAACTT ACAATTCAAA CAGGAGGTCG AGTGACACTT
    GAAGAGCCTA AACTTGAGAC AAGTAAATCA CCACGCTGCT CTATTAGTTC TGTTGCTGAT
    GTAGTTTTCC TTGTGGATGG ATCATGGAGT GTTGGAAGAA ACAACTTCAA ATATATTCTC
    GATTTCATGG TTTCTCTGGT CTCAGCCTTT GATATTGGGG AAGACAAAAC CCGAGTCGGT
    GTTGTGCAGT ATAGCTCTGA CACTAGGACT GAGTTTAACC TGAACAGTTA TTACAAGAAA
    GAGGAATTAG TATCAGCCAT AAAAAGAATA CCATACAAGG GAGGCAATAC AATGACAGGT
    GATGCCCTTG ATTACCTGAT TAAGAACTCC TTTGTCAAAA CTGCCGGCTC AAGAAAAGGA
    TTTCCAAGAA TAGCTATCAT TATAACTGAT GGTAAATCCC AAGATGAAGT GGAGATTCCA
    GCAAGAGAGC TGCGTAGCTT AGGGGTTGAA GTTTTTTCCC TTGGTATAAA AGCAGCAGAT
    GCAAAAGAAC TCAAACAAAT AGCTTCTCTG CCTTCCTTGA AACATGTTTT TAATGTTGCT
    AATTTTGATT CGATTGTGGA AGTGCAAGAT GAAATAATTA CTCAAGTTTG CTCAGGAGTT
    GAAGAGCAGC TAAGTGAGCT TGTTAGTGGA GAAGAAGCTG TTGAGCCTCC TTCAGATATG
    ACTGTTACGG AAATTTCTTC CAAATCAATG AAGATCACCT GGAGACCATC GCCAAGCCAA
    ATTACAGGCT ACAGGATTCA GCTTCTTCCA ATGTTAGCGG GAAGTAAACA ACATTCACTG
    AACCTTGGCC CTCAAACTAC AAGTATAAAT GTAAAAGATC TCTCCCCTGA TACAGAATAC
    CAAATAAATT TATATGCCAT GAAAGGACTC ACAGCAAGTG AGCCAATCTC AACACTGGAA
    AAAACCCAGG CTGTGAAAAT TAAAGTCGAA TGTTCCGGTG GTGTCGATAT AAAAGCAGAT
    GTCGTTCTGC TTGTTGATGG CTCCTACAGT ATTGGTGTAG CTAATTTTGC CAAAGTTAGA
    GCCTTCCTTG AAGTATTAGT AAAAAGCTTT GATATTTCGC CCAGCAAAGT ACAGATCAGT
    CTTGTGCAAT ACAGTAGAGA TCCATTTACA GAGTTTACCT TAAACAGGTA TGACTCAATT
    GAAGACATTC TCAAAGCTGT AAATACCTTT CCATATAGAG GTGGCTCTAC CAACACTGGC
    AAAGCAATGA CCTACGTGAG AGAAAAGGTC TTTGTTGAAA TTAAAGGAGC AAGACCAAAC
    GTCCCTAGAG TCATGATTCT TATCACCGAT GGAAAGTCAT CAGATGCCTT TAAAGATCCA
    GCTATAAAAC TGCGGAATTC AGATGTTGAG ATTTTTGCAG TTGGTGTGAA GGATGCAGTG
    AGATCTGAGC TGGAGGCCAT TGCTTCTCCT CCTTCAGACA CCCATGTGTA CACAGTGGAA
    GATTTTGATG CTTTTCAAAG GATTTCTTTT GAGCTAACAC AGTCTATCTG CCTTAGAATT
    GAGCAAGAGC TACAAACAAT AAAACGTAAA TCTTTGACCC CTGCAAGAGA TTTGGCTTTC
    TCTGAGGTTA CATCCAACAG TTTTCGTGTG AACTGGTCTC CTGCGGCTGA GGATGTTTTG
    TCATACCTTG TTAAATATAA GGTGGCTGTT GGAGAAGGTG AAGAATTCAT CGTTTCCGTG
    CCAGCACCAA AAACAACCAC AGTTCTTTCA AATTTACTTC CAGAAACTAC TTATGCTGTG
    GCTGTCCTGG CAGAATACGA GGAAGGAGAG AGTCCTCCAT TAAATGGGGA AGAAACAACT
    TTAGAAGTTA CTGGCCCACC AAGAAACCTG AGAGTAAGTG ACGAAACAAC TGATAGCTTT
    AAAGTTGGCT GGTCACCAGC TCCTGGCAAT GTACTGAGGT ACAGAATTGC CTACCGGCCT
    GTGGCAGGAG GGGCAAGAAG AGAGGTTACA GTCCCAGGAA GGGAGACCGT GACAACACTC
    CAGAACCTCA CACCTGATAC AAAGTACCAA GTATCTGTGA TTCCTGAATA TCATTCGGGT
    CCTGGAAAAC CTTTGACAGG AAATGGAGCC ACAGAAGAAG TGTTGGGAGA ACCAAGGAAT
    CTGAGGGTTT CTGATGCCAC AACCTCCAGT TTAAAACTGT CCTGGGATTC GGCACCAGGC
    AAAGTGTTAC AGTATCTTGT AACTTACAAC CCTTCTGCAG AAGGGGAAAC TAAAGAGGTT
    ACAGTAAGAG GAAATACAAC CAGTACAGTA CTGAAGGGAT TAGACCCAAG CACCACATAC
    TCCCTTTCTG TAGCTGCACT CTATAGATCT GGCGCTGGCT CATTACTTAC TGGGGAAGGA
    ACAACTCTTG ATGAACGTGG TTCTCCTCGT AACCTGGTAA CCAAAGACAT TACAGACACT
    ACAATTGGAG CATCATGGAC AGCAGCCCCA GGGATGGTTC GTGGTTACAG AATTGCATGG
    AAGTCTCTTT TTGATGATGA CTCTGGAGAA AAGAATGTTC CTGGTGGTAT TACTAACACT
    GTGCTGGAAA ACCTGAGGCC AGAAACAAAG TATAAAATTT CAGTGTTTGC TAATTATAGA
    AGTGGAGAAG GAGACCCTCT TGAAGGAGAA GCCACCACTG AAGCGTCACC AAGTGCCAAG
    CTGCTTAAAG TCAGTGATGA GACAGAAACT ACAATGAAGG TAACCTGGCA GCCTGCCCCA
    GGAAATGTTG CAAATTATCG GGTTATATAT CGGCCGAAGC TTGGAGGAAG GCAAGTGGTC
    GCTAAAGTGC CAGCTACAGT CTCCTCAACA GTACTGAAAA GGCTTCAACC TCAAACAACC
    TATGATATTA CAGTTGTTCC TGTTTATAAG GCAGGGGATG GAAAACCAAG ACAAGGAGAA
    GGGACAACAG CTTCTCCTTT CAAGCCCCCA AGGAATCTGA AAACATCTGA CCCCACAATC
    TCAACTTTCC GAGTCACCTG GGAACCTGCT CCTGGTGAAG TTAGGGGTTA TAAAATCACA
    TATTTTCCTT TTGGAGAGGA GCAATATTTG GGAGAGTTAG TAGTTGGACC ATATGATAAC
    ACTGTGGTAC TAGAAGAACT CAGGGCAGGA ACATCTTATA AAGTCAATGT ATTTGGAGTT
    TTTGAAAAAG GAGAGAGTGT ACCTTTAATT GGACAAGAAA TGACAACATT GTCAGATGCT
    CCTGAAGTTC GCATTGATTC GTCAGGCCTG GAATGCAAAA CTAAAGCTGC AGCTGATATA
    GTGTTGCTGG TGGATGGCTC TTGGAGTATC GGCCGCCCAA ATTTTAGGAC CGTCAGGAGT
    TTTATAGCAC GACTGGTTGA AGTCTTTGAT ATTGGCTCAG ACAGAGTGCA GATTGCCTTG
    GCCCAATACA GTGGGGACCC TCGATCTGAA TGGCAACTCA ATGCTCATAG CACAAAGAAG
    TCTTTAATGG ATGCCGTTGC AAACCTTCCT TACAAGGGGG GTAACACTCT TACAGGCATG
    GCTCTGAATT TCATCTTGCA AAATAACTTC AAGGCAGAGG CAGGCATGAG AGCAAAGTCA
    AAGAAAATTG GTGTCTTAAT CACTGATGGA AAGTCACAAG ATGACATTGT TGCACCTTCC
    AAAAAGTTAC GAGACCAGGG AATAGACTTA TTTGCAATTG GTATCAAAAA TGCTGATGAA
    AATGAACTGA GGCAGATTGG CTCAGATCCA GATGAGACTT ACGTATTTAA CGTTGCCGAT
    TTTTCTTTGC TTGTCAATAT TGTTGATGAC CTCACTACCA ATCTATGTAA CAGTGTGAAA
    GGCTCAGGAA ATGAACTGGA GCCACCTTCA GACCTAGTAA CATCAGAGCC TACACCACGG
    AGCTTCAGAG TCACATGGAA TCCACCTTCT CACAGTGTAG ACCGATTTAG GGTGGAATAT
    TATCCAGTGT CTGGTGGTCG ACCTCTGGAG GTATTTGTTG GCAGATCAGA TAGAACTACG
    GTTTTGACTA ATTTAAAGCC AGAAACAGAG TATGTTGTGA AAGTGTATTC TGTGTTAGAA
    GGAGAAAGCA GTGAACCATT AGAAGGCACA GAAACGACAT TGCCCATCCC TTCAGTCAGA
    AATCTTAACA TATATGATAT AGGAACCAGA ACAATGAGAG TTCGATGGGA AGCTGCGCTT
    GGAGCTACTG GTTATTTATT GCAGTATGAG CCAGTAAACG CTACTGTGCC TTCAACTGAA
    AAAGAGTTGA GAGTTGGTGC ATCGGTGACA GATGTACTTC TGGAAGATTT ATTCCCCAAC
    ACTGAGTACA CCTTGACTGT ATATGCACTT TTTGATGAGC TGATCAGTGA TCCTCTTACC
    TCACAGGAAG TCACTTTACC CATTCAAGGC GTGGGGAACA TTAGAGTGAG AGATATCACT
    CACAGCTCAA TGAATGTCCT TTGGAGTGCT GCTCCTGGAA ATGTTCTCAA ATATATAGTA
    AGATATAAAA CAGAGGAAGA AGAAGAATTC AAAGAGGTGG AAGTGGATGG ATCAAGGACA
    AGTACACGAC TTACTGACCT TTTCTCACAA ACTCTATATG AAATCAGTTT AACTGCAGTA
    TATGATGAAG GAGTATCTCA GCCACGTACT ACACAAGGAA CTACAGCACC AGTACCAGCT
    CCAGCAAACC TCCGGTTTTC TGAAATAGCA GTAACAAGTT TCCGAGGTAC TTGGGAGCAT
    GGGGCTCCAG ATGTAGCTTT GTACAGGATA ACGTGGGCAC CAGTTGGAGC CATAGAAAAA
    AAGAAAGAGA CAATTGTTGA TGGTGATATA AACACTTTGG TTTTTGATGA ACTGGAACCA
    GACACAGAGT ATGAGGTTTC AGTAACTGCG ATTTACCCTG ACGAATCAGA GAGTGAAGAT
    CTGATCGGTG TTGAAAGGAC ATTGCCTGAA ATAATTTCTA CAACACCTGC CCTAAGGACT
    GGCCCTAGGA ATCTACAGGT GTTCAATGCA ACACAGAATA GTTTGACTGT ACGATGGGAT
    CGTGCTATGG GCGATGTGCA AAAATACAAT ATCTATTATA AGCCTGTTTC TGCTGCTGGT
    GCTGAACAAA TGACAAGTGT TGGTGGAAAG CAGAACAGTG TTGTTCTTCA GCGACTGCAG
    CCAGACACTC CATACAACAT TGTTGTTTCA TCAAGGTACA TTGGAGATGA GGAAGGAGGA
    AATATCTCAG GGAGAGGAAA AACAAAGCCC TTAAATACAG TGAAGAATCT AAGAGTGTAT
    GACCCAACAA CAAGCACTTT AAATGTACGT TGGGAAGCTG CAGAAGGAAA TGTGCGGCGG
    TATAAAATTT ACTATGTACC AACTGCTGGA GGACCAGAGG AAACGGTGAC AGTGCCCGGA
    AATACAATCT ATTCAGTTCT TAGGAATCTT CAGTCTGATA CATCATATAC AGTAACTGTT
    GTTCCTGTCT ATTCTGAAGG AGAAGGAGGT CGTATGTCAG AAACAGGAAA GACTTTGGTG
    CGGGGAACAG TTCGAAATAT TCAAGTATAC AACCCCACAA CAAACACTTT GAATGTTCGC
    TGGGAGCCTG CGCCTGGACA GGTCCAGCAA TATAAAGTCA ACTATGCTCC ACTTCTTGGA
    ACAAGGCCTA CTGAGTCTGT TGTAGTTCCT GGCAATACTC ACAACGCGCT GCTGGAGCAA
    CTTATTCCTG ACACACAGTA CTCTGTAAAT GTTGTGGCTC TTTATGCAGA TGGTGAGGGG
    GATCCTAGCC CTGGTGAAGG AAAAACATTA CCTCGCAGTG GGCCAAAAAA TATTAGAGTT
    TTTGGAGAGA CCACTAACAG TCTCTCTGTG AACTGGGATC ATGCTGATGG ACCAGTCCAA
    CAATACAGAA TCATCTATTC ACCAACTGTT GGAGACCCCA TTGATGAATA TACAACAGTG
    CCTGGTAGGA GAAATAATGT GGTACTGCAG CCCCTTTTGT CTGACACGCC ATATAAAATT
    ACTGTGGTGG CAGTTTATGC TGATGGTGAT GGAGGACAAC TGACAGGAAA AGGAAGGACA
    GTGGGTTTGA TTGCACCACG GAACCTTCAA GTGTCTGATG AATGGTACAC AAGATTTAGG
    GTTTCCTGGG ATAGAATACC ATCTCCTGTT CTTGGATACA AGCTGGTTTA TCAGCCACGT
    GATTCAGATG AATCAATGGA AGTATTTGTC GGGGATGTGA GCTCCTACAC TTTGCAGAAC
    CTGTCTCCTG GCACCACATA TGATGTGAAA GTTTACGCTC AGTATGATAC AGGATTAAGT
    GGGCCCCTAT CAGGACCTGG GACAACTTTA TACTTAAATG TAACCGATTT GACAAGCTAT
    CAGGTTTCAT GGGACTCGTT CTGTATAAGA TGGACTCCTC ACCGATCAGC CACTTCCTAC
    AGACTGAAAC TGAATCCAGT TGATGGATCT CGAGGTCAAG AAATAACAGT AACAGGATCA
    GAATCATCAC ACTGTTTTAA TGGACTGAAC CAAGATACAC AGTACACAGC TACTGTTTTT
    GTGCAAACAC CTAACCTTGA AGGACGAGGA GTTTCAATAG ATGAGCGTAC ATTGATCAAG
    CCTACAGAAT CACCAACGAC ACCACCTGCT CCTCCTCCAC CCCCTACAAT TCCACCTGCT
    CGGGAGGTGT GCAAAGGGGC CAAAGCAGAC ATAGTATTCC TCATTGACAG TTCGTGGAGT
    ATTGGTGATG ATAACTACAA TAAAGTCCGT CAGTTTATCT TCAATACTAT TGGAGCATTC
    GATGTAATCA ACCCTTCTGG AATCCAGGTC TCTTTTGTGC AGTTCAGCGA TGATGCCCAG
    ACGGAGTTCA AGTTGAACAC TTATGCCGAC AAATCACAGG CTCTAGGAGC ACTTGCCAAT
    ATCAGATACA AAGGAGGAAA CACAAAAACA GGAAAGGCTC TTAAATTTGT CCACGAGAAA
    GTGATGACAG TTGAGAATGG AATGAGAAGA TCTGTTCCTA GGGTTCTGGT AGTGGTGACT
    GATGGCAGAT CTCAGGATGA TGTTAAGAAA TCAGCCTTGG ACATTCAGCA ATCTGGTTTT
    AGTGTCTTTG TCATTGGTGT GGCTGATGTG GATTATAATG AGCTAAGAAA CATTGGCAGT
    AAACCAAGCG AGCGCCATGT TTTTATAGTC GATGACTTTG ATGCTTTTGA GAAGATTGAA
    GATAACCTGA TCAACTTTGT ATGTGAGACT GCAACATCCA CTTGCCCACT TATTTACCTG
    GAAGGATACA CTTCACCAGG TTTCAGAATG CTGGAAGCAT ATAACCTGAC AGAGAAAACT
    TTTGCCTCAG TACAAGGTGT CTCAATGCAA CCAGGATCTT TCAACAGTTT CCCTGCATAC
    AACCTACATA AAGATGCCTT CATTTCCCTG CCTACCTCAG AATTGCACCC AGAAGGATTA
    CCACCTTCAT ATACGATTAT ATTGCTATTC CGTCTGCTGC CAGAAACTCC CAATGAGCCA
    TTTTCTCTAT GGCAAATAAC AGATAAAGGT TACAAGCCAC AAGTAGGAGT AAATCTTGAT
    CCATCTAAGA AAACACTGTC GTTCTTTAAT AAGGGAGCTG CTGGGGATAC TCAGACAGTA
    ACATTTGATG GGAATGAAGT GAAGAAACTC TTTTATGGAA GCTTCCACAA GGTTCTCATT
    GTGGTTTCTC CATCATACGT CAGAATTTAT ATTGATTGTA GTGAAGTTGC TGAAAAAGAA
    ATCAAAGAAG CTGGCAATAT TACAGCAGAA GGATATGAAG TACTTGGGAA GACATTAAAG
    GGTGATAAAA AATCTGCACC CTTCCTGCTC CAGATGTTTG ACATTGTGTG TACTGTGACC
    TGGACAAGTC GAGATAGATG CTGTGATATT CCTGCTACAA GGGATGAAGC CAAATGTCCA
    GCTCTGCCCC ATTCCTGTAC TTGCGCACAA GACAGCGTTG GAGCACCAGG ACCCCCAGGC
    CCATCCGGTA TTCCCGGTAG TAAAGGACCA CGGGGTGACC GAGGTTTGGA TGGACCAGTT
    GGCCCACCAG GTCAGCGAGG AGAACAAGGT TTGCCTGGTC CACAAGGACC ACCTGGACCT
    CAAGGACCAC CTGGACTTTC CATTCCTGGA GAACCTGGTC GTCAAGGAAT AAAAGGAGAT
    GCCGGTGACC CAGGATTGCC AGGAAGAATG GGTTCCCCAG GGGTGACAGG ACCACCAGGG
    CCTATGGGTC CACCAGGCAC AAGGGGTTTC ACAGGAAAGG ATGGTGCAGT AGGACCCAGA
    GGACCACCAG GACCAATGGG AGCACCTGGA CCTCAGGGAG TGCCAGGTCC AAGTGGGAAA
    CCAGGAAAGC AAGGAGATAA TGGATTGCCA GGTCCTCTTG GACAGAAAGG AGACAAGGGT
    GAGAGGGGAG ACATGGCATC TCAGAATATG ATGAGATCAG TAGCAAGACA AGTCTGTGAA
    CAGATGATAA ATAGTCAAAT GAGCAGATTT AATCAGATGT TGAACCAAAT TCCAAATGAT
    TACTATTCCA ATCGCAATCA GCCTGGTCCT CCAGGTCCTC CCGGTCCTCC AGGCAACGCT
    GGCATGAGAG GAGAACCTGG AGCTGCTGGC AGATCTGGAT TCCCAGGAAC ACCTGGTCCT
    CAAGGACCAC CTGGAGAAAG AGGAATACCA GGAGAGAAAG GGGAGAGAGG GAACCCAGGA
    ATTGGATCCC AAGGACCCAG GGGACTTCCA GGACCTCCAG GACCTCCAGG CGAATCCAGA
    ACAGGACCTC CAGGATCCTC AGGCTCCACA GGACCTCCAG GACCTTCAGG TCGCCCCGGT
    AACCAAGGAA TTAGAGGGCC TCCTGGTCCA GCTGGATACT GTGATGCTTC AATGTGTGCA
    AGCATTCCTT ACAATGGGCA AGGATACCCA GGTTCCGGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.