rhbdf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	rhbdf1
Entrez Gene ID	100494777
Full Name	rhomboid 5 homolog 1
General protein information	Preferred Names rhomboid 5 homolog 1 Names inactive rhomboid protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004917940.3	XP_004917997.1	inactive rhomboid protein 1 isoform X1
XM_004917940.4	XP_004917997.1	inactive rhomboid protein 1 isoform X1
XM_004917941.4	XP_004917998.1	inactive rhomboid protein 1 isoform X1
XM_002941196.5	XP_002941242.1	inactive rhomboid protein 1 isoform X2
XM_002941196.4	XP_002941242.1	inactive rhomboid protein 1 isoform X2
XM_004917941.3	XP_004917998.1	inactive rhomboid protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RHBDF1	XP_510701.3
Rattus norvegicus (Norway rat)	Rhbdf1	NP_001025205.1
Danio rerio (zebrafish)	rhbdf1	NP_001002228.1
Xenopus tropicalis (tropical clawed frog)	rhbdf1	XP_004917998.1
Homo sapiens (human)	RHBDF1	NP_071895.3
Gallus gallus (chicken)	RHBDF1	XP_004945406.1
Canis lupus familiaris (dog)	RHBDF1	XP_005621827.1
Bos taurus (cattle)	RHBDF1	NP_001098870.1
Mus musculus (house mouse)	Rhbdf1	NP_034247.2

Gene Ontology

The following rhbdf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rhbdf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa16272	XM_004917940.3 Latest version!	Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa16272	XM_004917941.3 Latest version!	Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa16273	XM_002941196.4 Latest version!	Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa67087	XM_004917941.4 Latest version!	Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa67087	XM_004917940.4 Latest version!	Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa67088	XM_002941196.5 Latest version!	Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rhbdf1 ( XM_004917940.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917997.1 Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa16272
Clone ID Related Accession (Same CDS sequence)	XM_004917940.3 , XM_004917941.3
Accession Version	XM_004917940.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917940.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAG TTCGACGTGA TAGTACAAGC AGCTTGCAAA GAAAGAAACC ACCATGGCTA 
AAGTTAGAAA TACCATCTGC AACTATTACA AGCGAGGAAC CTACATTCGT TCAGCCATTT 
AAGCGACAAA CATTTTTAAG AAGTGTGAGT ATGCCAGTTG AAAATACAAG AATTCCGTCA 
CCACCAAATG AACAGAAGCG ACCTAATTTA CAACGCCAGA CCTCAATCAC GCAGACTATT 
AAAAGGAAGC ACGTCATGTT TGAGAGAACC AGTACAATGC CTGTGAAGGG CCCACTTGCT 
GGCAGGAGAC GTATACGTTT AAGAAAGAGG CGAGCTTTCT CCACTTTATT TTGCAGGGGA 
ACTGCCGACT GGTTCGGAGT CAGCAAAGAC AATGACGCCA CACAAAAATG GCAAAGAAAA 
AGCCTTAGAC ATTGCAGCCA GAGATATGGA AAGTTAAAAC CCCAAGTTAT ACGAGAAATG 
GACCTTCCAA GTCAAGATAA CATATCCCTA ACAAGCACAG AAACTCCTCC CCCCCTTTAT 
GTTGGACCAA CTCAATTTAA TATGCAGAAG ATTATAGATC CTTTGGCCCG GGGACGTGCT 
TTCCGTATGG TAGATGACAA TGATGGCTAC AGCATGCCTC ATACTCCCAT TACGCCTGGT 
GCAGCTTCTT TGTGCTCATT TACCAGCTCA AGATCTGGAT TTAGTCGATA TCCCCGGAGA 
CGAAAAAAGG AATCGGTGGC TAAAATGAGT TTCAGAGCAG CAGCAGCACT TGTGAAAGGT 
CGTTCCATTA GGGAAGGTAC ATTACGTAGA GTACCAAGAC GAAGCTTCAC CCCAGCAAGT 
TTTCTGGAGG AAGATACAAT GGATTTCCCA GATGAATTAG ACACGTCATT TTTTGCAAGG 
GAAGGAGTTC TTCACGAGGA CCTCTCCAGT TATCCGGATG AAGTATTTGA ATCTCCATCT 
GAGGCTGCCA TCAAAGAGGC TGAACAGACA GCAAAATCAG AAGGGGTAGA ACGGACTGGA 
AGTGCTTTAG ACAAAAGTGA ACTTGAAAAA AGTCATCTGA TGCTGCCTTT GGAACGGGGA 
TGGCGGAAGC AAAAAGAAGG ACACATTTTA CAGCCAAAAG TACGACTTTG CCAGGAAGTT 
GTTAGTGTCA GTCCACAAAA GAGAGGACAA CGCATCACTA TTCCAGTAAA AAAACTCTTT 
GCAAAAGAAA AGAGGCCATA TGGTCTTGGC ATGGTGGGAA AACTGACTAA CAGAACATAC 
CGCAAAAGGA TAGACAGTTA TGTAAAAAGG CAAATAGAAG ATATGGATGA TCATAGGCCT 
TTCTTCACTT ATTGGATTAC AGTTGTGCAT TTACTTATAA CGATTCTTGC TGTATGCATC 
TATGGAATAG CACCAGTTGG CTTTTCTCAA CATGAAACTG TAGAATCGGT TCTTAGAAAT 
CGAGGTGTTT ATGAAAATGT AAAATATATT CAACAAGAAA ACTTTTGGAT TGGACCAAGT 
TCTGAAGCCT TAATACACTT GGGTGCAAAA TTTTCCCCAT GCATGAGACA AGACCAAGAT 
GTTTACAAAT TTATCAAAAT AAATAGAGAT AAAGAGAAGA ACTCAGCATG CTGTGTAAGA 
AATGACAAAT CAGGATGCAT ACAGACTTCT GAAGAAGAGT GCTCGTCTAC TCTTGCTGTT 
TGGGTAAAAT GGCCCACTCA TTCCAGTACA CCAGAACTGG CAAACAAAAG TGCAAGGCAT 
TCTGGATCAG TTTGCCACCA AGATCCAAGG TTCTGTGCTG AACCAGCATC AGTTACCCCA 
CACGAATGGC CGGATGACAT TACAAAATGG CCGGTCTGCA CAAAAAACAG TGACGGACAT 
TTCCCAAACC ATCTTCACAT GGACTGTGTT ATAACAGGCA GGCCCTGTTG TATTGGTACA 
AAAGGAAGGT GTGAAATAAC TTCCAGGGAA TATTGTGATT TTATGAAAGG ATACTTCCAT 
GAAGAGGCTA CATTGTGCTC ACAGGTGCAT TGCATGGATG ATGTGTGTGG ACTACTGCCA 
TTCTTGGACC CAGAGTTTCC AGATCAGGTC TATAGGTTAT GGCTTTCTCT CTTCCTGCAT 
GCAGGAGTCT TGCACTGTTT AGTTTCTGTT TGTTTTCAGA TGACAATACT GCGAGATTTG 
GAAAAGTTGG CAGGTTGGCA TAGAATATCC ATTATTTATA TACTAAGTGG AATTACTGGA 
AATCTAACAA GTGCTATTTT CCTGCCATAC AGGGCTGAGG TGGGGCCTGC TGGTTCACAG 
TTTGGAATTC TGGCATGCCT TTTTGTGGAA CTTTTCCAGA GCTGGCAAAT TCTTGCAAGA 
CCATGGAGAG CCTTTTTCAA GCTATTTGCA GTTGTTATTT TCCTGTTCAC CTTTGGACTC 
CTTCCTTGGA TTGACAATTT TGCTCATTTT GCTGGCTTTG TTAGTGGCTT CTTCTTATCC 
TTTGCATTTC TTCCTTATAT TAGCTTTGGC AAATTTGATA TGTACCGTAA ACGATGCCAG 
ATTATCATAT TCCTATTAAT ATTTTTTGGC CTTTTTTCAG GTTTAGTAGT TTTGTTTTAT 
GTGTACCCTA TCAAGTGTGA ATGGTGTGAA TACTTAACCT GTATACCATT TACAGATAAA 
TTCTGTGAAA AATATGAACT TCATGCACAG CTACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917997.1
CDS	420..3098
Translation	MSEVRRDSTSSLQRKKPPWLKLEIPSATITSEEPTFVQPFKRQTFLRSVSMPVENTRIPSPPNEQKRPNLQRQTSITQTIKRKHVMFERTSTMPVKGPLAGRRRIRLRKRRAFSTLFCRGTADWFGVSKDNDATQKWQRKSLRHCSQRYGKLKPQVIREMDLPSQDNISLTSTETPPPLYVGPTQFNMQKIIDPLARGRAFRMVDDNDGYSMPHTPITPGAASLCSFTSSRSGFSRYPRRRKKESVAKMSFRAAAALVKGRSIREGTLRRVPRRSFTPASFLEEDTMDFPDELDTSFFAREGVLHEDLSSYPDEVFESPSEAAIKEAEQTAKSEGVERTGSALDKSELEKSHLMLPLERGWRKQKEGHILQPKVRLCQEVVSVSPQKRGQRITIPVKKLFAKEKRPYGLGMVGKLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWITVVHLLITILAVCIYGIAPVGFSQHETVESVLRNRGVYENVKYIQQENFWIGPSSEALIHLGAKFSPCMRQDQDVYKFIKINRDKEKNSACCVRNDKSGCIQTSEEECSSTLAVWVKWPTHSSTPELANKSARHSGSVCHQDPRFCAEPASVTPHEWPDDITKWPVCTKNSDGHFPNHLHMDCVITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLCSQVHCMDDVCGLLPFLDPEFPDQVYRLWLSLFLHAGVLHCLVSVCFQMTILRDLEKLAGWHRISIIYILSGITGNLTSAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLFAVVIFLFTFGLLPWIDNFAHFAGFVSGFFLSFAFLPYISFGKFDMYRKRCQIIIFLLIFFGLFSGLVVLFYVYPIKCEWCEYLTCIPFTDKFCEKYELHAQLH

Target ORF information:

RefSeq Version	XM_004917940.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917940.3

ORF Insert Sequence:

ATGTCGGAAG TTCGACGTGA TAGTACAAGC AGCTTGCAAA GAAAGAAACC ACCATGGCTA 
AAGTTAGAAA TACCATCTGC AACTATTACA AGCGAGGAAC CTACATTCGT TCAGCCATTT 
AAGCGACAAA CATTTTTAAG AAGTGTGAGT ATGCCAGTTG AAAATACAAG AATTCCGTCA 
CCACCAAATG AACAGAAGCG ACCTAATTTA CAACGCCAGA CCTCAATCAC GCAGACTATT 
AAAAGGAAGC ACGTCATGTT TGAGAGAACC AGTACAATGC CTGTGAAGGG CCCACTTGCT 
GGCAGGAGAC GTATACGTTT AAGAAAGAGG CGAGCTTTCT CCACTTTATT TTGCAGGGGA 
ACTGCCGACT GGTTCGGAGT CAGCAAAGAC AATGACGCCA CACAAAAATG GCAAAGAAAA 
AGCCTTAGAC ATTGCAGCCA GAGATATGGA AAGTTAAAAC CCCAAGTTAT ACGAGAAATG 
GACCTTCCAA GTCAAGATAA CATATCCCTA ACAAGCACAG AAACTCCTCC CCCCCTTTAT 
GTTGGACCAA CTCAATTTAA TATGCAGAAG ATTATAGATC CTTTGGCCCG GGGACGTGCT 
TTCCGTATGG TAGATGACAA TGATGGCTAC AGCATGCCTC ATACTCCCAT TACGCCTGGT 
GCAGCTTCTT TGTGCTCATT TACCAGCTCA AGATCTGGAT TTAGTCGATA TCCCCGGAGA 
CGAAAAAAGG AATCGGTGGC TAAAATGAGT TTCAGAGCAG CAGCAGCACT TGTGAAAGGT 
CGTTCCATTA GGGAAGGTAC ATTACGTAGA GTACCAAGAC GAAGCTTCAC CCCAGCAAGT 
TTTCTGGAGG AAGATACAAT GGATTTCCCA GATGAATTAG ACACGTCATT TTTTGCAAGG 
GAAGGAGTTC TTCACGAGGA CCTCTCCAGT TATCCGGATG AAGTATTTGA ATCTCCATCT 
GAGGCTGCCA TCAAAGAGGC TGAACAGACA GCAAAATCAG AAGGGGTAGA ACGGACTGGA 
AGTGCTTTAG ACAAAAGTGA ACTTGAAAAA AGTCATCTGA TGCTGCCTTT GGAACGGGGA 
TGGCGGAAGC AAAAAGAAGG ACACATTTTA CAGCCAAAAG TACGACTTTG CCAGGAAGTT 
GTTAGTGTCA GTCCACAAAA GAGAGGACAA CGCATCACTA TTCCAGTAAA AAAACTCTTT 
GCAAAAGAAA AGAGGCCATA TGGTCTTGGC ATGGTGGGAA AACTGACTAA CAGAACATAC 
CGCAAAAGGA TAGACAGTTA TGTAAAAAGG CAAATAGAAG ATATGGATGA TCATAGGCCT 
TTCTTCACTT ATTGGATTAC AGTTGTGCAT TTACTTATAA CGATTCTTGC TGTATGCATC 
TATGGAATAG CACCAGTTGG CTTTTCTCAA CATGAAACTG TAGAATCGGT TCTTAGAAAT 
CGAGGTGTTT ATGAAAATGT AAAATATATT CAACAAGAAA ACTTTTGGAT TGGACCAAGT 
TCTGAAGCCT TAATACACTT GGGTGCAAAA TTTTCCCCAT GCATGAGACA AGACCAAGAT 
GTTTACAAAT TTATCAAAAT AAATAGAGAT AAAGAGAAGA ACTCAGCATG CTGTGTAAGA 
AATGACAAAT CAGGATGCAT ACAGACTTCT GAAGAAGAGT GCTCGTCTAC TCTTGCTGTT 
TGGGTAAAAT GGCCCACTCA TTCCAGTACA CCAGAACTGG CAAACAAAAG TGCAAGGCAT 
TCTGGATCAG TTTGCCACCA AGATCCAAGG TTCTGTGCTG AACCAGCATC AGTTACCCCA 
CACGAATGGC CGGATGACAT TACAAAATGG CCGGTCTGCA CAAAAAACAG TGACGGACAT 
TTCCCAAACC ATCTTCACAT GGACTGTGTT ATAACAGGCA GGCCCTGTTG TATTGGTACA 
AAAGGAAGGT GTGAAATAAC TTCCAGGGAA TATTGTGATT TTATGAAAGG ATACTTCCAT 
GAAGAGGCTA CATTGTGCTC ACAGGTGCAT TGCATGGATG ATGTGTGTGG ACTACTGCCA 
TTCTTGGACC CAGAGTTTCC AGATCAGGTC TATAGGTTAT GGCTTTCTCT CTTCCTGCAT 
GCAGGAGTCT TGCACTGTTT AGTTTCTGTT TGTTTTCAGA TGACAATACT GCGAGATTTG 
GAAAAGTTGG CAGGTTGGCA TAGAATATCC ATTATTTATA TACTAAGTGG AATTACTGGA 
AATCTAACAA GTGCTATTTT CCTGCCATAC AGGGCTGAGG TGGGGCCTGC TGGTTCACAG 
TTTGGAATTC TGGCATGCCT TTTTGTGGAA CTTTTCCAGA GCTGGCAAAT TCTTGCAAGA 
CCATGGAGAG CCTTTTTCAA GCTATTTGCA GTTGTTATTT TCCTGTTCAC CTTTGGACTC 
CTTCCTTGGA TTGACAATTT TGCTCATTTT GCTGGCTTTG TTAGTGGCTT CTTCTTATCC 
TTTGCATTTC TTCCTTATAT TAGCTTTGGC AAATTTGATA TGTACCGTAA ACGATGCCAG 
ATTATCATAT TCCTATTAAT ATTTTTTGGC CTTTTTTCAG GTTTAGTAGT TTTGTTTTAT 
GTGTACCCTA TCAAGTGTGA ATGGTGTGAA TACTTAACCT GTATACCATT TACAGATAAA 
TTCTGTGAAA AATATGAACT TCATGCACAG CTACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rhbdf1 ( XM_004917941.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917998.1 Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16272
Clone ID Related Accession (Same CDS sequence)	XM_004917940.3 , XM_004917941.3
Accession Version	XM_004917941.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917941.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAG TTCGACGTGA TAGTACAAGC AGCTTGCAAA GAAAGAAACC ACCATGGCTA 
AAGTTAGAAA TACCATCTGC AACTATTACA AGCGAGGAAC CTACATTCGT TCAGCCATTT 
AAGCGACAAA CATTTTTAAG AAGTGTGAGT ATGCCAGTTG AAAATACAAG AATTCCGTCA 
CCACCAAATG AACAGAAGCG ACCTAATTTA CAACGCCAGA CCTCAATCAC GCAGACTATT 
AAAAGGAAGC ACGTCATGTT TGAGAGAACC AGTACAATGC CTGTGAAGGG CCCACTTGCT 
GGCAGGAGAC GTATACGTTT AAGAAAGAGG CGAGCTTTCT CCACTTTATT TTGCAGGGGA 
ACTGCCGACT GGTTCGGAGT CAGCAAAGAC AATGACGCCA CACAAAAATG GCAAAGAAAA 
AGCCTTAGAC ATTGCAGCCA GAGATATGGA AAGTTAAAAC CCCAAGTTAT ACGAGAAATG 
GACCTTCCAA GTCAAGATAA CATATCCCTA ACAAGCACAG AAACTCCTCC CCCCCTTTAT 
GTTGGACCAA CTCAATTTAA TATGCAGAAG ATTATAGATC CTTTGGCCCG GGGACGTGCT 
TTCCGTATGG TAGATGACAA TGATGGCTAC AGCATGCCTC ATACTCCCAT TACGCCTGGT 
GCAGCTTCTT TGTGCTCATT TACCAGCTCA AGATCTGGAT TTAGTCGATA TCCCCGGAGA 
CGAAAAAAGG AATCGGTGGC TAAAATGAGT TTCAGAGCAG CAGCAGCACT TGTGAAAGGT 
CGTTCCATTA GGGAAGGTAC ATTACGTAGA GTACCAAGAC GAAGCTTCAC CCCAGCAAGT 
TTTCTGGAGG AAGATACAAT GGATTTCCCA GATGAATTAG ACACGTCATT TTTTGCAAGG 
GAAGGAGTTC TTCACGAGGA CCTCTCCAGT TATCCGGATG AAGTATTTGA ATCTCCATCT 
GAGGCTGCCA TCAAAGAGGC TGAACAGACA GCAAAATCAG AAGGGGTAGA ACGGACTGGA 
AGTGCTTTAG ACAAAAGTGA ACTTGAAAAA AGTCATCTGA TGCTGCCTTT GGAACGGGGA 
TGGCGGAAGC AAAAAGAAGG ACACATTTTA CAGCCAAAAG TACGACTTTG CCAGGAAGTT 
GTTAGTGTCA GTCCACAAAA GAGAGGACAA CGCATCACTA TTCCAGTAAA AAAACTCTTT 
GCAAAAGAAA AGAGGCCATA TGGTCTTGGC ATGGTGGGAA AACTGACTAA CAGAACATAC 
CGCAAAAGGA TAGACAGTTA TGTAAAAAGG CAAATAGAAG ATATGGATGA TCATAGGCCT 
TTCTTCACTT ATTGGATTAC AGTTGTGCAT TTACTTATAA CGATTCTTGC TGTATGCATC 
TATGGAATAG CACCAGTTGG CTTTTCTCAA CATGAAACTG TAGAATCGGT TCTTAGAAAT 
CGAGGTGTTT ATGAAAATGT AAAATATATT CAACAAGAAA ACTTTTGGAT TGGACCAAGT 
TCTGAAGCCT TAATACACTT GGGTGCAAAA TTTTCCCCAT GCATGAGACA AGACCAAGAT 
GTTTACAAAT TTATCAAAAT AAATAGAGAT AAAGAGAAGA ACTCAGCATG CTGTGTAAGA 
AATGACAAAT CAGGATGCAT ACAGACTTCT GAAGAAGAGT GCTCGTCTAC TCTTGCTGTT 
TGGGTAAAAT GGCCCACTCA TTCCAGTACA CCAGAACTGG CAAACAAAAG TGCAAGGCAT 
TCTGGATCAG TTTGCCACCA AGATCCAAGG TTCTGTGCTG AACCAGCATC AGTTACCCCA 
CACGAATGGC CGGATGACAT TACAAAATGG CCGGTCTGCA CAAAAAACAG TGACGGACAT 
TTCCCAAACC ATCTTCACAT GGACTGTGTT ATAACAGGCA GGCCCTGTTG TATTGGTACA 
AAAGGAAGGT GTGAAATAAC TTCCAGGGAA TATTGTGATT TTATGAAAGG ATACTTCCAT 
GAAGAGGCTA CATTGTGCTC ACAGGTGCAT TGCATGGATG ATGTGTGTGG ACTACTGCCA 
TTCTTGGACC CAGAGTTTCC AGATCAGGTC TATAGGTTAT GGCTTTCTCT CTTCCTGCAT 
GCAGGAGTCT TGCACTGTTT AGTTTCTGTT TGTTTTCAGA TGACAATACT GCGAGATTTG 
GAAAAGTTGG CAGGTTGGCA TAGAATATCC ATTATTTATA TACTAAGTGG AATTACTGGA 
AATCTAACAA GTGCTATTTT CCTGCCATAC AGGGCTGAGG TGGGGCCTGC TGGTTCACAG 
TTTGGAATTC TGGCATGCCT TTTTGTGGAA CTTTTCCAGA GCTGGCAAAT TCTTGCAAGA 
CCATGGAGAG CCTTTTTCAA GCTATTTGCA GTTGTTATTT TCCTGTTCAC CTTTGGACTC 
CTTCCTTGGA TTGACAATTT TGCTCATTTT GCTGGCTTTG TTAGTGGCTT CTTCTTATCC 
TTTGCATTTC TTCCTTATAT TAGCTTTGGC AAATTTGATA TGTACCGTAA ACGATGCCAG 
ATTATCATAT TCCTATTAAT ATTTTTTGGC CTTTTTTCAG GTTTAGTAGT TTTGTTTTAT 
GTGTACCCTA TCAAGTGTGA ATGGTGTGAA TACTTAACCT GTATACCATT TACAGATAAA 
TTCTGTGAAA AATATGAACT TCATGCACAG CTACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917998.1
CDS	106..2784
Translation	MSEVRRDSTSSLQRKKPPWLKLEIPSATITSEEPTFVQPFKRQTFLRSVSMPVENTRIPSPPNEQKRPNLQRQTSITQTIKRKHVMFERTSTMPVKGPLAGRRRIRLRKRRAFSTLFCRGTADWFGVSKDNDATQKWQRKSLRHCSQRYGKLKPQVIREMDLPSQDNISLTSTETPPPLYVGPTQFNMQKIIDPLARGRAFRMVDDNDGYSMPHTPITPGAASLCSFTSSRSGFSRYPRRRKKESVAKMSFRAAAALVKGRSIREGTLRRVPRRSFTPASFLEEDTMDFPDELDTSFFAREGVLHEDLSSYPDEVFESPSEAAIKEAEQTAKSEGVERTGSALDKSELEKSHLMLPLERGWRKQKEGHILQPKVRLCQEVVSVSPQKRGQRITIPVKKLFAKEKRPYGLGMVGKLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWITVVHLLITILAVCIYGIAPVGFSQHETVESVLRNRGVYENVKYIQQENFWIGPSSEALIHLGAKFSPCMRQDQDVYKFIKINRDKEKNSACCVRNDKSGCIQTSEEECSSTLAVWVKWPTHSSTPELANKSARHSGSVCHQDPRFCAEPASVTPHEWPDDITKWPVCTKNSDGHFPNHLHMDCVITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLCSQVHCMDDVCGLLPFLDPEFPDQVYRLWLSLFLHAGVLHCLVSVCFQMTILRDLEKLAGWHRISIIYILSGITGNLTSAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLFAVVIFLFTFGLLPWIDNFAHFAGFVSGFFLSFAFLPYISFGKFDMYRKRCQIIIFLLIFFGLFSGLVVLFYVYPIKCEWCEYLTCIPFTDKFCEKYELHAQLH

Target ORF information:

RefSeq Version	XM_004917941.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917941.3

ORF Insert Sequence:

ATGTCGGAAG TTCGACGTGA TAGTACAAGC AGCTTGCAAA GAAAGAAACC ACCATGGCTA 
AAGTTAGAAA TACCATCTGC AACTATTACA AGCGAGGAAC CTACATTCGT TCAGCCATTT 
AAGCGACAAA CATTTTTAAG AAGTGTGAGT ATGCCAGTTG AAAATACAAG AATTCCGTCA 
CCACCAAATG AACAGAAGCG ACCTAATTTA CAACGCCAGA CCTCAATCAC GCAGACTATT 
AAAAGGAAGC ACGTCATGTT TGAGAGAACC AGTACAATGC CTGTGAAGGG CCCACTTGCT 
GGCAGGAGAC GTATACGTTT AAGAAAGAGG CGAGCTTTCT CCACTTTATT TTGCAGGGGA 
ACTGCCGACT GGTTCGGAGT CAGCAAAGAC AATGACGCCA CACAAAAATG GCAAAGAAAA 
AGCCTTAGAC ATTGCAGCCA GAGATATGGA AAGTTAAAAC CCCAAGTTAT ACGAGAAATG 
GACCTTCCAA GTCAAGATAA CATATCCCTA ACAAGCACAG AAACTCCTCC CCCCCTTTAT 
GTTGGACCAA CTCAATTTAA TATGCAGAAG ATTATAGATC CTTTGGCCCG GGGACGTGCT 
TTCCGTATGG TAGATGACAA TGATGGCTAC AGCATGCCTC ATACTCCCAT TACGCCTGGT 
GCAGCTTCTT TGTGCTCATT TACCAGCTCA AGATCTGGAT TTAGTCGATA TCCCCGGAGA 
CGAAAAAAGG AATCGGTGGC TAAAATGAGT TTCAGAGCAG CAGCAGCACT TGTGAAAGGT 
CGTTCCATTA GGGAAGGTAC ATTACGTAGA GTACCAAGAC GAAGCTTCAC CCCAGCAAGT 
TTTCTGGAGG AAGATACAAT GGATTTCCCA GATGAATTAG ACACGTCATT TTTTGCAAGG 
GAAGGAGTTC TTCACGAGGA CCTCTCCAGT TATCCGGATG AAGTATTTGA ATCTCCATCT 
GAGGCTGCCA TCAAAGAGGC TGAACAGACA GCAAAATCAG AAGGGGTAGA ACGGACTGGA 
AGTGCTTTAG ACAAAAGTGA ACTTGAAAAA AGTCATCTGA TGCTGCCTTT GGAACGGGGA 
TGGCGGAAGC AAAAAGAAGG ACACATTTTA CAGCCAAAAG TACGACTTTG CCAGGAAGTT 
GTTAGTGTCA GTCCACAAAA GAGAGGACAA CGCATCACTA TTCCAGTAAA AAAACTCTTT 
GCAAAAGAAA AGAGGCCATA TGGTCTTGGC ATGGTGGGAA AACTGACTAA CAGAACATAC 
CGCAAAAGGA TAGACAGTTA TGTAAAAAGG CAAATAGAAG ATATGGATGA TCATAGGCCT 
TTCTTCACTT ATTGGATTAC AGTTGTGCAT TTACTTATAA CGATTCTTGC TGTATGCATC 
TATGGAATAG CACCAGTTGG CTTTTCTCAA CATGAAACTG TAGAATCGGT TCTTAGAAAT 
CGAGGTGTTT ATGAAAATGT AAAATATATT CAACAAGAAA ACTTTTGGAT TGGACCAAGT 
TCTGAAGCCT TAATACACTT GGGTGCAAAA TTTTCCCCAT GCATGAGACA AGACCAAGAT 
GTTTACAAAT TTATCAAAAT AAATAGAGAT AAAGAGAAGA ACTCAGCATG CTGTGTAAGA 
AATGACAAAT CAGGATGCAT ACAGACTTCT GAAGAAGAGT GCTCGTCTAC TCTTGCTGTT 
TGGGTAAAAT GGCCCACTCA TTCCAGTACA CCAGAACTGG CAAACAAAAG TGCAAGGCAT 
TCTGGATCAG TTTGCCACCA AGATCCAAGG TTCTGTGCTG AACCAGCATC AGTTACCCCA 
CACGAATGGC CGGATGACAT TACAAAATGG CCGGTCTGCA CAAAAAACAG TGACGGACAT 
TTCCCAAACC ATCTTCACAT GGACTGTGTT ATAACAGGCA GGCCCTGTTG TATTGGTACA 
AAAGGAAGGT GTGAAATAAC TTCCAGGGAA TATTGTGATT TTATGAAAGG ATACTTCCAT 
GAAGAGGCTA CATTGTGCTC ACAGGTGCAT TGCATGGATG ATGTGTGTGG ACTACTGCCA 
TTCTTGGACC CAGAGTTTCC AGATCAGGTC TATAGGTTAT GGCTTTCTCT CTTCCTGCAT 
GCAGGAGTCT TGCACTGTTT AGTTTCTGTT TGTTTTCAGA TGACAATACT GCGAGATTTG 
GAAAAGTTGG CAGGTTGGCA TAGAATATCC ATTATTTATA TACTAAGTGG AATTACTGGA 
AATCTAACAA GTGCTATTTT CCTGCCATAC AGGGCTGAGG TGGGGCCTGC TGGTTCACAG 
TTTGGAATTC TGGCATGCCT TTTTGTGGAA CTTTTCCAGA GCTGGCAAAT TCTTGCAAGA 
CCATGGAGAG CCTTTTTCAA GCTATTTGCA GTTGTTATTT TCCTGTTCAC CTTTGGACTC 
CTTCCTTGGA TTGACAATTT TGCTCATTTT GCTGGCTTTG TTAGTGGCTT CTTCTTATCC 
TTTGCATTTC TTCCTTATAT TAGCTTTGGC AAATTTGATA TGTACCGTAA ACGATGCCAG 
ATTATCATAT TCCTATTAAT ATTTTTTGGC CTTTTTTCAG GTTTAGTAGT TTTGTTTTAT 
GTGTACCCTA TCAAGTGTGA ATGGTGTGAA TACTTAACCT GTATACCATT TACAGATAAA 
TTCTGTGAAA AATATGAACT TCATGCACAG CTACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rhbdf1 ( XM_002941196.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941242.1 Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X3, mRNA.

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CloneID	OXa16273
Clone ID Related Accession (Same CDS sequence)	XM_002941196.4
Accession Version	XM_002941196.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive rhomboid protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002941196.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAG TTCGACGTGA TAGTACAAGC AGCTTGCAAA GAAAGAAACC ACCATGGCTA 
AAGTTAGAAA TACCATCTGC AACTATTACA AGCGAGGAAC CTACATTCGT TCAGCCATTT 
AAGCGACAAA CATTTTTAAG AAGTGTGAGT ATGCCAGTTG AAAATACAAG AATTCCGTCA 
CCACCAAATG AACAGAAGCG ACCTAATTTA CAACGCCAGA CCTCAATCAC GCAGACTATT 
AAAAGGGGAA CTGCCGACTG GTTCGGAGTC AGCAAAGACA ATGACGCCAC ACAAAAATGG 
CAAAGAAAAA GCCTTAGACA TTGCAGCCAG AGATATGGAA AGTTAAAACC CCAAGTTATA 
CGAGAAATGG ACCTTCCAAG TCAAGATAAC ATATCCCTAA CAAGCACAGA AACTCCTCCC 
CCCCTTTATG TTGGACCAAC TCAATTTAAT ATGCAGAAGA TTATAGATCC TTTGGCCCGG 
GGACGTGCTT TCCGTATGGT AGATGACAAT GATGGCTACA GCATGCCTCA TACTCCCATT 
ACGCCTGGTG CAGCTTCTTT GTGCTCATTT ACCAGCTCAA GATCTGGATT TAGTCGATAT 
CCCCGGAGAC GAAAAAAGGA ATCGGTGGCT AAAATGAGTT TCAGAGCAGC AGCAGCACTT 
GTGAAAGGTC GTTCCATTAG GGAAGGTACA TTACGTAGAG TACCAAGACG AAGCTTCACC 
CCAGCAAGTT TTCTGGAGGA AGATACAATG GATTTCCCAG ATGAATTAGA CACGTCATTT 
TTTGCAAGGG AAGGAGTTCT TCACGAGGAC CTCTCCAGTT ATCCGGATGA AGTATTTGAA 
TCTCCATCTG AGGCTGCCAT CAAAGAGGCT GAACAGACAG CAAAATCAGA AGGGGTAGAA 
CGGACTGGAA GTGCTTTAGA CAAAAGTGAA CTTGAAAAAA GTCATCTGAT GCTGCCTTTG 
GAACGGGGAT GGCGGAAGCA AAAAGAAGGA CACATTTTAC AGCCAAAAGT ACGACTTTGC 
CAGGAAGTTG TTAGTGTCAG TCCACAAAAG AGAGGACAAC GCATCACTAT TCCAGTAAAA 
AAACTCTTTG CAAAAGAAAA GAGGCCATAT GGTCTTGGCA TGGTGGGAAA ACTGACTAAC 
AGAACATACC GCAAAAGGAT AGACAGTTAT GTAAAAAGGC AAATAGAAGA TATGGATGAT 
CATAGGCCTT TCTTCACTTA TTGGATTACA GTTGTGCATT TACTTATAAC GATTCTTGCT 
GTATGCATCT ATGGAATAGC ACCAGTTGGC TTTTCTCAAC ATGAAACTGT AGAATCGGTT 
CTTAGAAATC GAGGTGTTTA TGAAAATGTA AAATATATTC AACAAGAAAA CTTTTGGATT 
GGACCAAGTT CTGAAGCCTT AATACACTTG GGTGCAAAAT TTTCCCCATG CATGAGACAA 
GACCAAGATG TTTACAAATT TATCAAAATA AATAGAGATA AAGAGAAGAA CTCAGCATGC 
TGTGTAAGAA ATGACAAATC AGGATGCATA CAGACTTCTG AAGAAGAGTG CTCGTCTACT 
CTTGCTGTTT GGGTAAAATG GCCCACTCAT TCCAGTACAC CAGAACTGGC AAACAAAAGT 
GCAAGGCATT CTGGATCAGT TTGCCACCAA GATCCAAGGT TCTGTGCTGA ACCAGCATCA 
GTTACCCCAC ACGAATGGCC GGATGACATT ACAAAATGGC CGGTCTGCAC AAAAAACAGT 
GACGGACATT TCCCAAACCA TCTTCACATG GACTGTGTTA TAACAGGCAG GCCCTGTTGT 
ATTGGTACAA AAGGAAGGTG TGAAATAACT TCCAGGGAAT ATTGTGATTT TATGAAAGGA 
TACTTCCATG AAGAGGCTAC ATTGTGCTCA CAGGTGCATT GCATGGATGA TGTGTGTGGA 
CTACTGCCAT TCTTGGACCC AGAGTTTCCA GATCAGGTCT ATAGGTTATG GCTTTCTCTC 
TTCCTGCATG CAGGAGTCTT GCACTGTTTA GTTTCTGTTT GTTTTCAGAT GACAATACTG 
CGAGATTTGG AAAAGTTGGC AGGTTGGCAT AGAATATCCA TTATTTATAT ACTAAGTGGA 
ATTACTGGAA ATCTAACAAG TGCTATTTTC CTGCCATACA GGGCTGAGGT GGGGCCTGCT 
GGTTCACAGT TTGGAATTCT GGCATGCCTT TTTGTGGAAC TTTTCCAGAG CTGGCAAATT 
CTTGCAAGAC CATGGAGAGC CTTTTTCAAG CTATTTGCAG TTGTTATTTT CCTGTTCACC 
TTTGGACTCC TTCCTTGGAT TGACAATTTT GCTCATTTTG CTGGCTTTGT TAGTGGCTTC 
TTCTTATCCT TTGCATTTCT TCCTTATATT AGCTTTGGCA AATTTGATAT GTACCGTAAA 
CGATGCCAGA TTATCATATT CCTATTAATA TTTTTTGGCC TTTTTTCAGG TTTAGTAGTT 
TTGTTTTATG TGTACCCTAT CAAGTGTGAA TGGTGTGAAT ACTTAACCTG TATACCATTT 
ACAGATAAAT TCTGTGAAAA ATATGAACTT CATGCACAGC TACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941242.1
CDS	415..2982
Translation	MSEVRRDSTSSLQRKKPPWLKLEIPSATITSEEPTFVQPFKRQTFLRSVSMPVENTRIPSPPNEQKRPNLQRQTSITQTIKRGTADWFGVSKDNDATQKWQRKSLRHCSQRYGKLKPQVIREMDLPSQDNISLTSTETPPPLYVGPTQFNMQKIIDPLARGRAFRMVDDNDGYSMPHTPITPGAASLCSFTSSRSGFSRYPRRRKKESVAKMSFRAAAALVKGRSIREGTLRRVPRRSFTPASFLEEDTMDFPDELDTSFFAREGVLHEDLSSYPDEVFESPSEAAIKEAEQTAKSEGVERTGSALDKSELEKSHLMLPLERGWRKQKEGHILQPKVRLCQEVVSVSPQKRGQRITIPVKKLFAKEKRPYGLGMVGKLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWITVVHLLITILAVCIYGIAPVGFSQHETVESVLRNRGVYENVKYIQQENFWIGPSSEALIHLGAKFSPCMRQDQDVYKFIKINRDKEKNSACCVRNDKSGCIQTSEEECSSTLAVWVKWPTHSSTPELANKSARHSGSVCHQDPRFCAEPASVTPHEWPDDITKWPVCTKNSDGHFPNHLHMDCVITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLCSQVHCMDDVCGLLPFLDPEFPDQVYRLWLSLFLHAGVLHCLVSVCFQMTILRDLEKLAGWHRISIIYILSGITGNLTSAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLFAVVIFLFTFGLLPWIDNFAHFAGFVSGFFLSFAFLPYISFGKFDMYRKRCQIIIFLLIFFGLFSGLVVLFYVYPIKCEWCEYLTCIPFTDKFCEKYELHAQLH

Target ORF information:

RefSeq Version	XM_002941196.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002941196.4

ORF Insert Sequence:

ATGTCGGAAG TTCGACGTGA TAGTACAAGC AGCTTGCAAA GAAAGAAACC ACCATGGCTA 
AAGTTAGAAA TACCATCTGC AACTATTACA AGCGAGGAAC CTACATTCGT TCAGCCATTT 
AAGCGACAAA CATTTTTAAG AAGTGTGAGT ATGCCAGTTG AAAATACAAG AATTCCGTCA 
CCACCAAATG AACAGAAGCG ACCTAATTTA CAACGCCAGA CCTCAATCAC GCAGACTATT 
AAAAGGGGAA CTGCCGACTG GTTCGGAGTC AGCAAAGACA ATGACGCCAC ACAAAAATGG 
CAAAGAAAAA GCCTTAGACA TTGCAGCCAG AGATATGGAA AGTTAAAACC CCAAGTTATA 
CGAGAAATGG ACCTTCCAAG TCAAGATAAC ATATCCCTAA CAAGCACAGA AACTCCTCCC 
CCCCTTTATG TTGGACCAAC TCAATTTAAT ATGCAGAAGA TTATAGATCC TTTGGCCCGG 
GGACGTGCTT TCCGTATGGT AGATGACAAT GATGGCTACA GCATGCCTCA TACTCCCATT 
ACGCCTGGTG CAGCTTCTTT GTGCTCATTT ACCAGCTCAA GATCTGGATT TAGTCGATAT 
CCCCGGAGAC GAAAAAAGGA ATCGGTGGCT AAAATGAGTT TCAGAGCAGC AGCAGCACTT 
GTGAAAGGTC GTTCCATTAG GGAAGGTACA TTACGTAGAG TACCAAGACG AAGCTTCACC 
CCAGCAAGTT TTCTGGAGGA AGATACAATG GATTTCCCAG ATGAATTAGA CACGTCATTT 
TTTGCAAGGG AAGGAGTTCT TCACGAGGAC CTCTCCAGTT ATCCGGATGA AGTATTTGAA 
TCTCCATCTG AGGCTGCCAT CAAAGAGGCT GAACAGACAG CAAAATCAGA AGGGGTAGAA 
CGGACTGGAA GTGCTTTAGA CAAAAGTGAA CTTGAAAAAA GTCATCTGAT GCTGCCTTTG 
GAACGGGGAT GGCGGAAGCA AAAAGAAGGA CACATTTTAC AGCCAAAAGT ACGACTTTGC 
CAGGAAGTTG TTAGTGTCAG TCCACAAAAG AGAGGACAAC GCATCACTAT TCCAGTAAAA 
AAACTCTTTG CAAAAGAAAA GAGGCCATAT GGTCTTGGCA TGGTGGGAAA ACTGACTAAC 
AGAACATACC GCAAAAGGAT AGACAGTTAT GTAAAAAGGC AAATAGAAGA TATGGATGAT 
CATAGGCCTT TCTTCACTTA TTGGATTACA GTTGTGCATT TACTTATAAC GATTCTTGCT 
GTATGCATCT ATGGAATAGC ACCAGTTGGC TTTTCTCAAC ATGAAACTGT AGAATCGGTT 
CTTAGAAATC GAGGTGTTTA TGAAAATGTA AAATATATTC AACAAGAAAA CTTTTGGATT 
GGACCAAGTT CTGAAGCCTT AATACACTTG GGTGCAAAAT TTTCCCCATG CATGAGACAA 
GACCAAGATG TTTACAAATT TATCAAAATA AATAGAGATA AAGAGAAGAA CTCAGCATGC 
TGTGTAAGAA ATGACAAATC AGGATGCATA CAGACTTCTG AAGAAGAGTG CTCGTCTACT 
CTTGCTGTTT GGGTAAAATG GCCCACTCAT TCCAGTACAC CAGAACTGGC AAACAAAAGT 
GCAAGGCATT CTGGATCAGT TTGCCACCAA GATCCAAGGT TCTGTGCTGA ACCAGCATCA 
GTTACCCCAC ACGAATGGCC GGATGACATT ACAAAATGGC CGGTCTGCAC AAAAAACAGT 
GACGGACATT TCCCAAACCA TCTTCACATG GACTGTGTTA TAACAGGCAG GCCCTGTTGT 
ATTGGTACAA AAGGAAGGTG TGAAATAACT TCCAGGGAAT ATTGTGATTT TATGAAAGGA 
TACTTCCATG AAGAGGCTAC ATTGTGCTCA CAGGTGCATT GCATGGATGA TGTGTGTGGA 
CTACTGCCAT TCTTGGACCC AGAGTTTCCA GATCAGGTCT ATAGGTTATG GCTTTCTCTC 
TTCCTGCATG CAGGAGTCTT GCACTGTTTA GTTTCTGTTT GTTTTCAGAT GACAATACTG 
CGAGATTTGG AAAAGTTGGC AGGTTGGCAT AGAATATCCA TTATTTATAT ACTAAGTGGA 
ATTACTGGAA ATCTAACAAG TGCTATTTTC CTGCCATACA GGGCTGAGGT GGGGCCTGCT 
GGTTCACAGT TTGGAATTCT GGCATGCCTT TTTGTGGAAC TTTTCCAGAG CTGGCAAATT 
CTTGCAAGAC CATGGAGAGC CTTTTTCAAG CTATTTGCAG TTGTTATTTT CCTGTTCACC 
TTTGGACTCC TTCCTTGGAT TGACAATTTT GCTCATTTTG CTGGCTTTGT TAGTGGCTTC 
TTCTTATCCT TTGCATTTCT TCCTTATATT AGCTTTGGCA AATTTGATAT GTACCGTAAA 
CGATGCCAGA TTATCATATT CCTATTAATA TTTTTTGGCC TTTTTTCAGG TTTAGTAGTT 
TTGTTTTATG TGTACCCTAT CAAGTGTGAA TGGTGTGAAT ACTTAACCTG TATACCATTT 
ACAGATAAAT TCTGTGAAAA ATATGAACTT CATGCACAGC TACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rhbdf1 ( XM_004917940.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917997.1 Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa67087
Clone ID Related Accession (Same CDS sequence)	XM_004917940.4 , XM_004917941.4
Accession Version	XM_004917940.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917940.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAG TTCGACGTGA TAGTACAAGC AGCTTGCAAA GAAAGAAACC ACCATGGCTA 
AAGTTAGAAA TACCATCTGC AACTATTACA AGCGAGGAAC CTACATTCGT TCAGCCATTT 
AAGCGACAAA CATTTTTAAG AAGTGTGAGT ATGCCAGTTG AAAATACAAG AATTCCGTCA 
CCACCAAATG AACAGAAGCG ACCTAATTTA CAACGCCAGA CCTCAATCAC GCAGACTATT 
AAAAGGAAGC ACGTCATGTT TGAGAGAACC AGTACAATGC CTGTGAAGGG CCCACTTGCT 
GGCAGGAGAC GTATACGTTT AAGAAAGAGG CGAGCTTTCT CCACTTTATT TTGCAGGGGA 
ACTGCCGACT GGTTCGGAGT CAGCAAAGAC AATGACGCCA CACAAAAATG GCAAAGAAAA 
AGCCTTAGAC ATTGCAGCCA GAGATATGGA AAGTTAAAAC CCCAAGTTAT ACGAGAAATG 
GACCTTCCAA GTCAAGATAA CATATCCCTA ACAAGCACAG AAACTCCTCC CCCCCTTTAT 
GTTGGACCAA CTCAATTTAA TATGCAGAAG ATTATAGATC CTTTGGCCCG GGGACGTGCT 
TTCCGTATGG TAGATGACAA TGATGGCTAC AGCATGCCTC ATACTCCCAT TACGCCTGGT 
GCAGCTTCTT TGTGCTCATT TACCAGCTCA AGATCTGGAT TTAGTCGATA TCCCCGGAGA 
CGAAAAAAGG AATCGGTGGC TAAAATGAGT TTCAGAGCAG CAGCAGCACT TGTGAAAGGT 
CGTTCCATTA GGGAAGGTAC ATTACGTAGA GTACCAAGAC GAAGCTTCAC CCCAGCAAGT 
TTTCTGGAGG AAGATACAAT GGATTTCCCA GATGAATTAG ACACGTCATT TTTTGCAAGG 
GAAGGAGTTC TTCACGAGGA CCTCTCCAGT TATCCGGATG AAGTATTTGA ATCTCCATCT 
GAGGCTGCTA TCAAAGAGGC TGAACAGACA GCAAAATCAG AAGGGGTAGA ACGGACTGGA 
AGTGCTTTAG ACAAAAGTGA ACTTGAAAAA AGTCATCTGA TGCTGCCTTT GGAACGGGGA 
TGGCGGAAGC AAAAAGAAGG ACACATTTTA CAGCCAAAAG TACGACTTTG CCAGGAAGTT 
GTTAGTGTCA GTCCACAAAA GAGAGGACAA CGCATCACTA TTCCAGTAAA AAAACTCTTT 
GCAAAAGAAA AGAGGCCATA TGGTCTTGGC ATGGTGGGAA AACTGACTAA CAGAACATAC 
CGCAAAAGGA TAGACAGTTA TGTAAAAAGG CAAATAGAAG ATATGGATGA TCATAGGCCT 
TTCTTCACTT ATTGGATTAC AGTTGTGCAT TTACTTATAA CGATTCTTGC TGTATGCATC 
TATGGAATAG CACCAGTTGG CTTTTCTCAA CATGAAACTG TAGAATCGGT TCTTAGAAAT 
CGAGGTGTTT ATGAAAATGT AAAATATATT CAACAAGAAA ACTTTTGGAT TGGACCAAGT 
TCTGAAGCCT TAATACACTT GGGTGCAAAA TTTTCCCCAT GCATGAGACA AGACCAAGAT 
GTTTACAAAT TTATCAAAAT AAATAGAGAT AAAGAGAAGA ACTCAGCATG CTGTGTAAGA 
AATGACAAAT CAGGATGCAT ACAGACTTCT GAAGAAGAGT GCTCGTCTAC TCTTGCTGTT 
TGGGTAAAAT GGCCCACTCA TTCCAGTACA CCAGAACTGG CAAACAAAAG TGCAAGGCAT 
TCTGGATCAG TTTGCCACCA AGATCCAAGG TTCTGTGCTG AACCAGCATC AGTTACCCCA 
CACGAATGGC CGGATGACAT TACAAAATGG CCGGTCTGCA CAAAAAACAG TGACGGACAT 
TTCCCAAACC ATCTTCACAT GGACTGTGTT ATAACAGGCA GGCCCTGTTG TATTGGTACA 
AAAGGAAGGT GTGAAATAAC TTCCAGGGAA TATTGTGATT TTATGAAAGG ATACTTCCAT 
GAAGAGGCTA CATTGTGCTC ACAGGTGCAT TGCATGGATG ATGTGTGTGG ACTACTGCCA 
TTCTTGGACC CAGAGTTTCC AGATCAGGTC TATAGGTTAT GGCTTTCTCT CTTCCTGCAT 
GCAGGAGTCT TGCACTGTTT AGTTTCTGTT TGTTTTCAGA TGACAATACT GCGAGATTTG 
GAAAAGTTGG CAGGTTGGCA TAGAATATCC ATTATTTATA TACTAAGTGG AATTACTGGA 
AATCTAACAA GTGCTATTTT CCTGCCATAC AGGGCTGAGG TGGGGCCTGC TGGTTCACAG 
TTTGGAATTC TGGCATGCCT TTTTGTGGAA CTTTTCCAGA GCTGGCAAAT TCTTGCAAGA 
CCATGGAGAG CCTTTTTCAA GCTATTTGCA GTTGTTATTT TCCTGTTCAC CTTTGGACTC 
CTTCCTTGGA TTGACAATTT TGCTCATTTT GCTGGCTTTG TTAGTGGCTT CTTCTTATCC 
TTTGCATTTC TTCCTTATAT TAGCTTTGGC AAATTTGATA TGTACCGTAA ACGATGCCAG 
ATTATCATAT TCCTATTAAT ATTTTTTGGC CTTTTTTCAG GTTTAGTAGT TTTGTTTTAT 
GTGTACCCTA TCAAGTGTGA ATGGTGTGAA TACTTAACCT GTATACCATT TACAGATAAA 
TTCTGTGAAA AATATGAACT TCATGCACAG CTACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917997.1
CDS	571..3249
Translation	MSEVRRDSTSSLQRKKPPWLKLEIPSATITSEEPTFVQPFKRQTFLRSVSMPVENTRIPSPPNEQKRPNLQRQTSITQTIKRKHVMFERTSTMPVKGPLAGRRRIRLRKRRAFSTLFCRGTADWFGVSKDNDATQKWQRKSLRHCSQRYGKLKPQVIREMDLPSQDNISLTSTETPPPLYVGPTQFNMQKIIDPLARGRAFRMVDDNDGYSMPHTPITPGAASLCSFTSSRSGFSRYPRRRKKESVAKMSFRAAAALVKGRSIREGTLRRVPRRSFTPASFLEEDTMDFPDELDTSFFAREGVLHEDLSSYPDEVFESPSEAAIKEAEQTAKSEGVERTGSALDKSELEKSHLMLPLERGWRKQKEGHILQPKVRLCQEVVSVSPQKRGQRITIPVKKLFAKEKRPYGLGMVGKLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWITVVHLLITILAVCIYGIAPVGFSQHETVESVLRNRGVYENVKYIQQENFWIGPSSEALIHLGAKFSPCMRQDQDVYKFIKINRDKEKNSACCVRNDKSGCIQTSEEECSSTLAVWVKWPTHSSTPELANKSARHSGSVCHQDPRFCAEPASVTPHEWPDDITKWPVCTKNSDGHFPNHLHMDCVITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLCSQVHCMDDVCGLLPFLDPEFPDQVYRLWLSLFLHAGVLHCLVSVCFQMTILRDLEKLAGWHRISIIYILSGITGNLTSAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLFAVVIFLFTFGLLPWIDNFAHFAGFVSGFFLSFAFLPYISFGKFDMYRKRCQIIIFLLIFFGLFSGLVVLFYVYPIKCEWCEYLTCIPFTDKFCEKYELHAQLH

Target ORF information:

RefSeq Version	XM_004917940.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917940.4

ORF Insert Sequence:

ATGTCGGAAG TTCGACGTGA TAGTACAAGC AGCTTGCAAA GAAAGAAACC ACCATGGCTA 
AAGTTAGAAA TACCATCTGC AACTATTACA AGCGAGGAAC CTACATTCGT TCAGCCATTT 
AAGCGACAAA CATTTTTAAG AAGTGTGAGT ATGCCAGTTG AAAATACAAG AATTCCGTCA 
CCACCAAATG AACAGAAGCG ACCTAATTTA CAACGCCAGA CCTCAATCAC GCAGACTATT 
AAAAGGAAGC ACGTCATGTT TGAGAGAACC AGTACAATGC CTGTGAAGGG CCCACTTGCT 
GGCAGGAGAC GTATACGTTT AAGAAAGAGG CGAGCTTTCT CCACTTTATT TTGCAGGGGA 
ACTGCCGACT GGTTCGGAGT CAGCAAAGAC AATGACGCCA CACAAAAATG GCAAAGAAAA 
AGCCTTAGAC ATTGCAGCCA GAGATATGGA AAGTTAAAAC CCCAAGTTAT ACGAGAAATG 
GACCTTCCAA GTCAAGATAA CATATCCCTA ACAAGCACAG AAACTCCTCC CCCCCTTTAT 
GTTGGACCAA CTCAATTTAA TATGCAGAAG ATTATAGATC CTTTGGCCCG GGGACGTGCT 
TTCCGTATGG TAGATGACAA TGATGGCTAC AGCATGCCTC ATACTCCCAT TACGCCTGGT 
GCAGCTTCTT TGTGCTCATT TACCAGCTCA AGATCTGGAT TTAGTCGATA TCCCCGGAGA 
CGAAAAAAGG AATCGGTGGC TAAAATGAGT TTCAGAGCAG CAGCAGCACT TGTGAAAGGT 
CGTTCCATTA GGGAAGGTAC ATTACGTAGA GTACCAAGAC GAAGCTTCAC CCCAGCAAGT 
TTTCTGGAGG AAGATACAAT GGATTTCCCA GATGAATTAG ACACGTCATT TTTTGCAAGG 
GAAGGAGTTC TTCACGAGGA CCTCTCCAGT TATCCGGATG AAGTATTTGA ATCTCCATCT 
GAGGCTGCTA TCAAAGAGGC TGAACAGACA GCAAAATCAG AAGGGGTAGA ACGGACTGGA 
AGTGCTTTAG ACAAAAGTGA ACTTGAAAAA AGTCATCTGA TGCTGCCTTT GGAACGGGGA 
TGGCGGAAGC AAAAAGAAGG ACACATTTTA CAGCCAAAAG TACGACTTTG CCAGGAAGTT 
GTTAGTGTCA GTCCACAAAA GAGAGGACAA CGCATCACTA TTCCAGTAAA AAAACTCTTT 
GCAAAAGAAA AGAGGCCATA TGGTCTTGGC ATGGTGGGAA AACTGACTAA CAGAACATAC 
CGCAAAAGGA TAGACAGTTA TGTAAAAAGG CAAATAGAAG ATATGGATGA TCATAGGCCT 
TTCTTCACTT ATTGGATTAC AGTTGTGCAT TTACTTATAA CGATTCTTGC TGTATGCATC 
TATGGAATAG CACCAGTTGG CTTTTCTCAA CATGAAACTG TAGAATCGGT TCTTAGAAAT 
CGAGGTGTTT ATGAAAATGT AAAATATATT CAACAAGAAA ACTTTTGGAT TGGACCAAGT 
TCTGAAGCCT TAATACACTT GGGTGCAAAA TTTTCCCCAT GCATGAGACA AGACCAAGAT 
GTTTACAAAT TTATCAAAAT AAATAGAGAT AAAGAGAAGA ACTCAGCATG CTGTGTAAGA 
AATGACAAAT CAGGATGCAT ACAGACTTCT GAAGAAGAGT GCTCGTCTAC TCTTGCTGTT 
TGGGTAAAAT GGCCCACTCA TTCCAGTACA CCAGAACTGG CAAACAAAAG TGCAAGGCAT 
TCTGGATCAG TTTGCCACCA AGATCCAAGG TTCTGTGCTG AACCAGCATC AGTTACCCCA 
CACGAATGGC CGGATGACAT TACAAAATGG CCGGTCTGCA CAAAAAACAG TGACGGACAT 
TTCCCAAACC ATCTTCACAT GGACTGTGTT ATAACAGGCA GGCCCTGTTG TATTGGTACA 
AAAGGAAGGT GTGAAATAAC TTCCAGGGAA TATTGTGATT TTATGAAAGG ATACTTCCAT 
GAAGAGGCTA CATTGTGCTC ACAGGTGCAT TGCATGGATG ATGTGTGTGG ACTACTGCCA 
TTCTTGGACC CAGAGTTTCC AGATCAGGTC TATAGGTTAT GGCTTTCTCT CTTCCTGCAT 
GCAGGAGTCT TGCACTGTTT AGTTTCTGTT TGTTTTCAGA TGACAATACT GCGAGATTTG 
GAAAAGTTGG CAGGTTGGCA TAGAATATCC ATTATTTATA TACTAAGTGG AATTACTGGA 
AATCTAACAA GTGCTATTTT CCTGCCATAC AGGGCTGAGG TGGGGCCTGC TGGTTCACAG 
TTTGGAATTC TGGCATGCCT TTTTGTGGAA CTTTTCCAGA GCTGGCAAAT TCTTGCAAGA 
CCATGGAGAG CCTTTTTCAA GCTATTTGCA GTTGTTATTT TCCTGTTCAC CTTTGGACTC 
CTTCCTTGGA TTGACAATTT TGCTCATTTT GCTGGCTTTG TTAGTGGCTT CTTCTTATCC 
TTTGCATTTC TTCCTTATAT TAGCTTTGGC AAATTTGATA TGTACCGTAA ACGATGCCAG 
ATTATCATAT TCCTATTAAT ATTTTTTGGC CTTTTTTCAG GTTTAGTAGT TTTGTTTTAT 
GTGTACCCTA TCAAGTGTGA ATGGTGTGAA TACTTAACCT GTATACCATT TACAGATAAA 
TTCTGTGAAA AATATGAACT TCATGCACAG CTACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rhbdf1 ( XM_004917941.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917998.1 Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X2, mRNA.

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CloneID	OXa67087
Clone ID Related Accession (Same CDS sequence)	XM_004917940.4 , XM_004917941.4
Accession Version	XM_004917941.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917941.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAG TTCGACGTGA TAGTACAAGC AGCTTGCAAA GAAAGAAACC ACCATGGCTA 
AAGTTAGAAA TACCATCTGC AACTATTACA AGCGAGGAAC CTACATTCGT TCAGCCATTT 
AAGCGACAAA CATTTTTAAG AAGTGTGAGT ATGCCAGTTG AAAATACAAG AATTCCGTCA 
CCACCAAATG AACAGAAGCG ACCTAATTTA CAACGCCAGA CCTCAATCAC GCAGACTATT 
AAAAGGAAGC ACGTCATGTT TGAGAGAACC AGTACAATGC CTGTGAAGGG CCCACTTGCT 
GGCAGGAGAC GTATACGTTT AAGAAAGAGG CGAGCTTTCT CCACTTTATT TTGCAGGGGA 
ACTGCCGACT GGTTCGGAGT CAGCAAAGAC AATGACGCCA CACAAAAATG GCAAAGAAAA 
AGCCTTAGAC ATTGCAGCCA GAGATATGGA AAGTTAAAAC CCCAAGTTAT ACGAGAAATG 
GACCTTCCAA GTCAAGATAA CATATCCCTA ACAAGCACAG AAACTCCTCC CCCCCTTTAT 
GTTGGACCAA CTCAATTTAA TATGCAGAAG ATTATAGATC CTTTGGCCCG GGGACGTGCT 
TTCCGTATGG TAGATGACAA TGATGGCTAC AGCATGCCTC ATACTCCCAT TACGCCTGGT 
GCAGCTTCTT TGTGCTCATT TACCAGCTCA AGATCTGGAT TTAGTCGATA TCCCCGGAGA 
CGAAAAAAGG AATCGGTGGC TAAAATGAGT TTCAGAGCAG CAGCAGCACT TGTGAAAGGT 
CGTTCCATTA GGGAAGGTAC ATTACGTAGA GTACCAAGAC GAAGCTTCAC CCCAGCAAGT 
TTTCTGGAGG AAGATACAAT GGATTTCCCA GATGAATTAG ACACGTCATT TTTTGCAAGG 
GAAGGAGTTC TTCACGAGGA CCTCTCCAGT TATCCGGATG AAGTATTTGA ATCTCCATCT 
GAGGCTGCTA TCAAAGAGGC TGAACAGACA GCAAAATCAG AAGGGGTAGA ACGGACTGGA 
AGTGCTTTAG ACAAAAGTGA ACTTGAAAAA AGTCATCTGA TGCTGCCTTT GGAACGGGGA 
TGGCGGAAGC AAAAAGAAGG ACACATTTTA CAGCCAAAAG TACGACTTTG CCAGGAAGTT 
GTTAGTGTCA GTCCACAAAA GAGAGGACAA CGCATCACTA TTCCAGTAAA AAAACTCTTT 
GCAAAAGAAA AGAGGCCATA TGGTCTTGGC ATGGTGGGAA AACTGACTAA CAGAACATAC 
CGCAAAAGGA TAGACAGTTA TGTAAAAAGG CAAATAGAAG ATATGGATGA TCATAGGCCT 
TTCTTCACTT ATTGGATTAC AGTTGTGCAT TTACTTATAA CGATTCTTGC TGTATGCATC 
TATGGAATAG CACCAGTTGG CTTTTCTCAA CATGAAACTG TAGAATCGGT TCTTAGAAAT 
CGAGGTGTTT ATGAAAATGT AAAATATATT CAACAAGAAA ACTTTTGGAT TGGACCAAGT 
TCTGAAGCCT TAATACACTT GGGTGCAAAA TTTTCCCCAT GCATGAGACA AGACCAAGAT 
GTTTACAAAT TTATCAAAAT AAATAGAGAT AAAGAGAAGA ACTCAGCATG CTGTGTAAGA 
AATGACAAAT CAGGATGCAT ACAGACTTCT GAAGAAGAGT GCTCGTCTAC TCTTGCTGTT 
TGGGTAAAAT GGCCCACTCA TTCCAGTACA CCAGAACTGG CAAACAAAAG TGCAAGGCAT 
TCTGGATCAG TTTGCCACCA AGATCCAAGG TTCTGTGCTG AACCAGCATC AGTTACCCCA 
CACGAATGGC CGGATGACAT TACAAAATGG CCGGTCTGCA CAAAAAACAG TGACGGACAT 
TTCCCAAACC ATCTTCACAT GGACTGTGTT ATAACAGGCA GGCCCTGTTG TATTGGTACA 
AAAGGAAGGT GTGAAATAAC TTCCAGGGAA TATTGTGATT TTATGAAAGG ATACTTCCAT 
GAAGAGGCTA CATTGTGCTC ACAGGTGCAT TGCATGGATG ATGTGTGTGG ACTACTGCCA 
TTCTTGGACC CAGAGTTTCC AGATCAGGTC TATAGGTTAT GGCTTTCTCT CTTCCTGCAT 
GCAGGAGTCT TGCACTGTTT AGTTTCTGTT TGTTTTCAGA TGACAATACT GCGAGATTTG 
GAAAAGTTGG CAGGTTGGCA TAGAATATCC ATTATTTATA TACTAAGTGG AATTACTGGA 
AATCTAACAA GTGCTATTTT CCTGCCATAC AGGGCTGAGG TGGGGCCTGC TGGTTCACAG 
TTTGGAATTC TGGCATGCCT TTTTGTGGAA CTTTTCCAGA GCTGGCAAAT TCTTGCAAGA 
CCATGGAGAG CCTTTTTCAA GCTATTTGCA GTTGTTATTT TCCTGTTCAC CTTTGGACTC 
CTTCCTTGGA TTGACAATTT TGCTCATTTT GCTGGCTTTG TTAGTGGCTT CTTCTTATCC 
TTTGCATTTC TTCCTTATAT TAGCTTTGGC AAATTTGATA TGTACCGTAA ACGATGCCAG 
ATTATCATAT TCCTATTAAT ATTTTTTGGC CTTTTTTCAG GTTTAGTAGT TTTGTTTTAT 
GTGTACCCTA TCAAGTGTGA ATGGTGTGAA TACTTAACCT GTATACCATT TACAGATAAA 
TTCTGTGAAA AATATGAACT TCATGCACAG CTACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917998.1
CDS	333..3011
Translation	MSEVRRDSTSSLQRKKPPWLKLEIPSATITSEEPTFVQPFKRQTFLRSVSMPVENTRIPSPPNEQKRPNLQRQTSITQTIKRKHVMFERTSTMPVKGPLAGRRRIRLRKRRAFSTLFCRGTADWFGVSKDNDATQKWQRKSLRHCSQRYGKLKPQVIREMDLPSQDNISLTSTETPPPLYVGPTQFNMQKIIDPLARGRAFRMVDDNDGYSMPHTPITPGAASLCSFTSSRSGFSRYPRRRKKESVAKMSFRAAAALVKGRSIREGTLRRVPRRSFTPASFLEEDTMDFPDELDTSFFAREGVLHEDLSSYPDEVFESPSEAAIKEAEQTAKSEGVERTGSALDKSELEKSHLMLPLERGWRKQKEGHILQPKVRLCQEVVSVSPQKRGQRITIPVKKLFAKEKRPYGLGMVGKLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWITVVHLLITILAVCIYGIAPVGFSQHETVESVLRNRGVYENVKYIQQENFWIGPSSEALIHLGAKFSPCMRQDQDVYKFIKINRDKEKNSACCVRNDKSGCIQTSEEECSSTLAVWVKWPTHSSTPELANKSARHSGSVCHQDPRFCAEPASVTPHEWPDDITKWPVCTKNSDGHFPNHLHMDCVITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLCSQVHCMDDVCGLLPFLDPEFPDQVYRLWLSLFLHAGVLHCLVSVCFQMTILRDLEKLAGWHRISIIYILSGITGNLTSAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLFAVVIFLFTFGLLPWIDNFAHFAGFVSGFFLSFAFLPYISFGKFDMYRKRCQIIIFLLIFFGLFSGLVVLFYVYPIKCEWCEYLTCIPFTDKFCEKYELHAQLH

Target ORF information:

RefSeq Version	XM_004917941.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917941.4

ORF Insert Sequence:

ATGTCGGAAG TTCGACGTGA TAGTACAAGC AGCTTGCAAA GAAAGAAACC ACCATGGCTA 
AAGTTAGAAA TACCATCTGC AACTATTACA AGCGAGGAAC CTACATTCGT TCAGCCATTT 
AAGCGACAAA CATTTTTAAG AAGTGTGAGT ATGCCAGTTG AAAATACAAG AATTCCGTCA 
CCACCAAATG AACAGAAGCG ACCTAATTTA CAACGCCAGA CCTCAATCAC GCAGACTATT 
AAAAGGAAGC ACGTCATGTT TGAGAGAACC AGTACAATGC CTGTGAAGGG CCCACTTGCT 
GGCAGGAGAC GTATACGTTT AAGAAAGAGG CGAGCTTTCT CCACTTTATT TTGCAGGGGA 
ACTGCCGACT GGTTCGGAGT CAGCAAAGAC AATGACGCCA CACAAAAATG GCAAAGAAAA 
AGCCTTAGAC ATTGCAGCCA GAGATATGGA AAGTTAAAAC CCCAAGTTAT ACGAGAAATG 
GACCTTCCAA GTCAAGATAA CATATCCCTA ACAAGCACAG AAACTCCTCC CCCCCTTTAT 
GTTGGACCAA CTCAATTTAA TATGCAGAAG ATTATAGATC CTTTGGCCCG GGGACGTGCT 
TTCCGTATGG TAGATGACAA TGATGGCTAC AGCATGCCTC ATACTCCCAT TACGCCTGGT 
GCAGCTTCTT TGTGCTCATT TACCAGCTCA AGATCTGGAT TTAGTCGATA TCCCCGGAGA 
CGAAAAAAGG AATCGGTGGC TAAAATGAGT TTCAGAGCAG CAGCAGCACT TGTGAAAGGT 
CGTTCCATTA GGGAAGGTAC ATTACGTAGA GTACCAAGAC GAAGCTTCAC CCCAGCAAGT 
TTTCTGGAGG AAGATACAAT GGATTTCCCA GATGAATTAG ACACGTCATT TTTTGCAAGG 
GAAGGAGTTC TTCACGAGGA CCTCTCCAGT TATCCGGATG AAGTATTTGA ATCTCCATCT 
GAGGCTGCTA TCAAAGAGGC TGAACAGACA GCAAAATCAG AAGGGGTAGA ACGGACTGGA 
AGTGCTTTAG ACAAAAGTGA ACTTGAAAAA AGTCATCTGA TGCTGCCTTT GGAACGGGGA 
TGGCGGAAGC AAAAAGAAGG ACACATTTTA CAGCCAAAAG TACGACTTTG CCAGGAAGTT 
GTTAGTGTCA GTCCACAAAA GAGAGGACAA CGCATCACTA TTCCAGTAAA AAAACTCTTT 
GCAAAAGAAA AGAGGCCATA TGGTCTTGGC ATGGTGGGAA AACTGACTAA CAGAACATAC 
CGCAAAAGGA TAGACAGTTA TGTAAAAAGG CAAATAGAAG ATATGGATGA TCATAGGCCT 
TTCTTCACTT ATTGGATTAC AGTTGTGCAT TTACTTATAA CGATTCTTGC TGTATGCATC 
TATGGAATAG CACCAGTTGG CTTTTCTCAA CATGAAACTG TAGAATCGGT TCTTAGAAAT 
CGAGGTGTTT ATGAAAATGT AAAATATATT CAACAAGAAA ACTTTTGGAT TGGACCAAGT 
TCTGAAGCCT TAATACACTT GGGTGCAAAA TTTTCCCCAT GCATGAGACA AGACCAAGAT 
GTTTACAAAT TTATCAAAAT AAATAGAGAT AAAGAGAAGA ACTCAGCATG CTGTGTAAGA 
AATGACAAAT CAGGATGCAT ACAGACTTCT GAAGAAGAGT GCTCGTCTAC TCTTGCTGTT 
TGGGTAAAAT GGCCCACTCA TTCCAGTACA CCAGAACTGG CAAACAAAAG TGCAAGGCAT 
TCTGGATCAG TTTGCCACCA AGATCCAAGG TTCTGTGCTG AACCAGCATC AGTTACCCCA 
CACGAATGGC CGGATGACAT TACAAAATGG CCGGTCTGCA CAAAAAACAG TGACGGACAT 
TTCCCAAACC ATCTTCACAT GGACTGTGTT ATAACAGGCA GGCCCTGTTG TATTGGTACA 
AAAGGAAGGT GTGAAATAAC TTCCAGGGAA TATTGTGATT TTATGAAAGG ATACTTCCAT 
GAAGAGGCTA CATTGTGCTC ACAGGTGCAT TGCATGGATG ATGTGTGTGG ACTACTGCCA 
TTCTTGGACC CAGAGTTTCC AGATCAGGTC TATAGGTTAT GGCTTTCTCT CTTCCTGCAT 
GCAGGAGTCT TGCACTGTTT AGTTTCTGTT TGTTTTCAGA TGACAATACT GCGAGATTTG 
GAAAAGTTGG CAGGTTGGCA TAGAATATCC ATTATTTATA TACTAAGTGG AATTACTGGA 
AATCTAACAA GTGCTATTTT CCTGCCATAC AGGGCTGAGG TGGGGCCTGC TGGTTCACAG 
TTTGGAATTC TGGCATGCCT TTTTGTGGAA CTTTTCCAGA GCTGGCAAAT TCTTGCAAGA 
CCATGGAGAG CCTTTTTCAA GCTATTTGCA GTTGTTATTT TCCTGTTCAC CTTTGGACTC 
CTTCCTTGGA TTGACAATTT TGCTCATTTT GCTGGCTTTG TTAGTGGCTT CTTCTTATCC 
TTTGCATTTC TTCCTTATAT TAGCTTTGGC AAATTTGATA TGTACCGTAA ACGATGCCAG 
ATTATCATAT TCCTATTAAT ATTTTTTGGC CTTTTTTCAG GTTTAGTAGT TTTGTTTTAT 
GTGTACCCTA TCAAGTGTGA ATGGTGTGAA TACTTAACCT GTATACCATT TACAGATAAA 
TTCTGTGAAA AATATGAACT TCATGCACAG CTACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rhbdf1 ( XM_002941196.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941242.1 Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa67088
Clone ID Related Accession (Same CDS sequence)	XM_002941196.5
Accession Version	XM_002941196.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive rhomboid protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002941196.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAAG TTCGACGTGA TAGTACAAGC AGCTTGCAAA GAAAGAAACC ACCATGGCTA 
AAGTTAGAAA TACCATCTGC AACTATTACA AGCGAGGAAC CTACATTCGT TCAGCCATTT 
AAGCGACAAA CATTTTTAAG AAGTGTGAGT ATGCCAGTTG AAAATACAAG AATTCCGTCA 
CCACCAAATG AACAGAAGCG ACCTAATTTA CAACGCCAGA CCTCAATCAC GCAGACTATT 
AAAAGGGGAA CTGCCGACTG GTTCGGAGTC AGCAAAGACA ATGACGCCAC ACAAAAATGG 
CAAAGAAAAA GCCTTAGACA TTGCAGCCAG AGATATGGAA AGTTAAAACC CCAAGTTATA 
CGAGAAATGG ACCTTCCAAG TCAAGATAAC ATATCCCTAA CAAGCACAGA AACTCCTCCC 
CCCCTTTATG TTGGACCAAC TCAATTTAAT ATGCAGAAGA TTATAGATCC TTTGGCCCGG 
GGACGTGCTT TCCGTATGGT AGATGACAAT GATGGCTACA GCATGCCTCA TACTCCCATT 
ACGCCTGGTG CAGCTTCTTT GTGCTCATTT ACCAGCTCAA GATCTGGATT TAGTCGATAT 
CCCCGGAGAC GAAAAAAGGA ATCGGTGGCT AAAATGAGTT TCAGAGCAGC AGCAGCACTT 
GTGAAAGGTC GTTCCATTAG GGAAGGTACA TTACGTAGAG TACCAAGACG AAGCTTCACC 
CCAGCAAGTT TTCTGGAGGA AGATACAATG GATTTCCCAG ATGAATTAGA CACGTCATTT 
TTTGCAAGGG AAGGAGTTCT TCACGAGGAC CTCTCCAGTT ATCCGGATGA AGTATTTGAA 
TCTCCATCTG AGGCTGCTAT CAAAGAGGCT GAACAGACAG CAAAATCAGA AGGGGTAGAA 
CGGACTGGAA GTGCTTTAGA CAAAAGTGAA CTTGAAAAAA GTCATCTGAT GCTGCCTTTG 
GAACGGGGAT GGCGGAAGCA AAAAGAAGGA CACATTTTAC AGCCAAAAGT ACGACTTTGC 
CAGGAAGTTG TTAGTGTCAG TCCACAAAAG AGAGGACAAC GCATCACTAT TCCAGTAAAA 
AAACTCTTTG CAAAAGAAAA GAGGCCATAT GGTCTTGGCA TGGTGGGAAA ACTGACTAAC 
AGAACATACC GCAAAAGGAT AGACAGTTAT GTAAAAAGGC AAATAGAAGA TATGGATGAT 
CATAGGCCTT TCTTCACTTA TTGGATTACA GTTGTGCATT TACTTATAAC GATTCTTGCT 
GTATGCATCT ATGGAATAGC ACCAGTTGGC TTTTCTCAAC ATGAAACTGT AGAATCGGTT 
CTTAGAAATC GAGGTGTTTA TGAAAATGTA AAATATATTC AACAAGAAAA CTTTTGGATT 
GGACCAAGTT CTGAAGCCTT AATACACTTG GGTGCAAAAT TTTCCCCATG CATGAGACAA 
GACCAAGATG TTTACAAATT TATCAAAATA AATAGAGATA AAGAGAAGAA CTCAGCATGC 
TGTGTAAGAA ATGACAAATC AGGATGCATA CAGACTTCTG AAGAAGAGTG CTCGTCTACT 
CTTGCTGTTT GGGTAAAATG GCCCACTCAT TCCAGTACAC CAGAACTGGC AAACAAAAGT 
GCAAGGCATT CTGGATCAGT TTGCCACCAA GATCCAAGGT TCTGTGCTGA ACCAGCATCA 
GTTACCCCAC ACGAATGGCC GGATGACATT ACAAAATGGC CGGTCTGCAC AAAAAACAGT 
GACGGACATT TCCCAAACCA TCTTCACATG GACTGTGTTA TAACAGGCAG GCCCTGTTGT 
ATTGGTACAA AAGGAAGGTG TGAAATAACT TCCAGGGAAT ATTGTGATTT TATGAAAGGA 
TACTTCCATG AAGAGGCTAC ATTGTGCTCA CAGGTGCATT GCATGGATGA TGTGTGTGGA 
CTACTGCCAT TCTTGGACCC AGAGTTTCCA GATCAGGTCT ATAGGTTATG GCTTTCTCTC 
TTCCTGCATG CAGGAGTCTT GCACTGTTTA GTTTCTGTTT GTTTTCAGAT GACAATACTG 
CGAGATTTGG AAAAGTTGGC AGGTTGGCAT AGAATATCCA TTATTTATAT ACTAAGTGGA 
ATTACTGGAA ATCTAACAAG TGCTATTTTC CTGCCATACA GGGCTGAGGT GGGGCCTGCT 
GGTTCACAGT TTGGAATTCT GGCATGCCTT TTTGTGGAAC TTTTCCAGAG CTGGCAAATT 
CTTGCAAGAC CATGGAGAGC CTTTTTCAAG CTATTTGCAG TTGTTATTTT CCTGTTCACC 
TTTGGACTCC TTCCTTGGAT TGACAATTTT GCTCATTTTG CTGGCTTTGT TAGTGGCTTC 
TTCTTATCCT TTGCATTTCT TCCTTATATT AGCTTTGGCA AATTTGATAT GTACCGTAAA 
CGATGCCAGA TTATCATATT CCTATTAATA TTTTTTGGCC TTTTTTCAGG TTTAGTAGTT 
TTGTTTTATG TGTACCCTAT CAAGTGTGAA TGGTGTGAAT ACTTAACCTG TATACCATTT 
ACAGATAAAT TCTGTGAAAA ATATGAACTT CATGCACAGC TACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941242.1
CDS	568..3135
Translation	MSEVRRDSTSSLQRKKPPWLKLEIPSATITSEEPTFVQPFKRQTFLRSVSMPVENTRIPSPPNEQKRPNLQRQTSITQTIKRGTADWFGVSKDNDATQKWQRKSLRHCSQRYGKLKPQVIREMDLPSQDNISLTSTETPPPLYVGPTQFNMQKIIDPLARGRAFRMVDDNDGYSMPHTPITPGAASLCSFTSSRSGFSRYPRRRKKESVAKMSFRAAAALVKGRSIREGTLRRVPRRSFTPASFLEEDTMDFPDELDTSFFAREGVLHEDLSSYPDEVFESPSEAAIKEAEQTAKSEGVERTGSALDKSELEKSHLMLPLERGWRKQKEGHILQPKVRLCQEVVSVSPQKRGQRITIPVKKLFAKEKRPYGLGMVGKLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWITVVHLLITILAVCIYGIAPVGFSQHETVESVLRNRGVYENVKYIQQENFWIGPSSEALIHLGAKFSPCMRQDQDVYKFIKINRDKEKNSACCVRNDKSGCIQTSEEECSSTLAVWVKWPTHSSTPELANKSARHSGSVCHQDPRFCAEPASVTPHEWPDDITKWPVCTKNSDGHFPNHLHMDCVITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLCSQVHCMDDVCGLLPFLDPEFPDQVYRLWLSLFLHAGVLHCLVSVCFQMTILRDLEKLAGWHRISIIYILSGITGNLTSAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLFAVVIFLFTFGLLPWIDNFAHFAGFVSGFFLSFAFLPYISFGKFDMYRKRCQIIIFLLIFFGLFSGLVVLFYVYPIKCEWCEYLTCIPFTDKFCEKYELHAQLH

Target ORF information:

RefSeq Version	XM_002941196.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis rhomboid 5 homolog 1 (rhbdf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002941196.5

ORF Insert Sequence:

ATGTCGGAAG TTCGACGTGA TAGTACAAGC AGCTTGCAAA GAAAGAAACC ACCATGGCTA 
AAGTTAGAAA TACCATCTGC AACTATTACA AGCGAGGAAC CTACATTCGT TCAGCCATTT 
AAGCGACAAA CATTTTTAAG AAGTGTGAGT ATGCCAGTTG AAAATACAAG AATTCCGTCA 
CCACCAAATG AACAGAAGCG ACCTAATTTA CAACGCCAGA CCTCAATCAC GCAGACTATT 
AAAAGGGGAA CTGCCGACTG GTTCGGAGTC AGCAAAGACA ATGACGCCAC ACAAAAATGG 
CAAAGAAAAA GCCTTAGACA TTGCAGCCAG AGATATGGAA AGTTAAAACC CCAAGTTATA 
CGAGAAATGG ACCTTCCAAG TCAAGATAAC ATATCCCTAA CAAGCACAGA AACTCCTCCC 
CCCCTTTATG TTGGACCAAC TCAATTTAAT ATGCAGAAGA TTATAGATCC TTTGGCCCGG 
GGACGTGCTT TCCGTATGGT AGATGACAAT GATGGCTACA GCATGCCTCA TACTCCCATT 
ACGCCTGGTG CAGCTTCTTT GTGCTCATTT ACCAGCTCAA GATCTGGATT TAGTCGATAT 
CCCCGGAGAC GAAAAAAGGA ATCGGTGGCT AAAATGAGTT TCAGAGCAGC AGCAGCACTT 
GTGAAAGGTC GTTCCATTAG GGAAGGTACA TTACGTAGAG TACCAAGACG AAGCTTCACC 
CCAGCAAGTT TTCTGGAGGA AGATACAATG GATTTCCCAG ATGAATTAGA CACGTCATTT 
TTTGCAAGGG AAGGAGTTCT TCACGAGGAC CTCTCCAGTT ATCCGGATGA AGTATTTGAA 
TCTCCATCTG AGGCTGCTAT CAAAGAGGCT GAACAGACAG CAAAATCAGA AGGGGTAGAA 
CGGACTGGAA GTGCTTTAGA CAAAAGTGAA CTTGAAAAAA GTCATCTGAT GCTGCCTTTG 
GAACGGGGAT GGCGGAAGCA AAAAGAAGGA CACATTTTAC AGCCAAAAGT ACGACTTTGC 
CAGGAAGTTG TTAGTGTCAG TCCACAAAAG AGAGGACAAC GCATCACTAT TCCAGTAAAA 
AAACTCTTTG CAAAAGAAAA GAGGCCATAT GGTCTTGGCA TGGTGGGAAA ACTGACTAAC 
AGAACATACC GCAAAAGGAT AGACAGTTAT GTAAAAAGGC AAATAGAAGA TATGGATGAT 
CATAGGCCTT TCTTCACTTA TTGGATTACA GTTGTGCATT TACTTATAAC GATTCTTGCT 
GTATGCATCT ATGGAATAGC ACCAGTTGGC TTTTCTCAAC ATGAAACTGT AGAATCGGTT 
CTTAGAAATC GAGGTGTTTA TGAAAATGTA AAATATATTC AACAAGAAAA CTTTTGGATT 
GGACCAAGTT CTGAAGCCTT AATACACTTG GGTGCAAAAT TTTCCCCATG CATGAGACAA 
GACCAAGATG TTTACAAATT TATCAAAATA AATAGAGATA AAGAGAAGAA CTCAGCATGC 
TGTGTAAGAA ATGACAAATC AGGATGCATA CAGACTTCTG AAGAAGAGTG CTCGTCTACT 
CTTGCTGTTT GGGTAAAATG GCCCACTCAT TCCAGTACAC CAGAACTGGC AAACAAAAGT 
GCAAGGCATT CTGGATCAGT TTGCCACCAA GATCCAAGGT TCTGTGCTGA ACCAGCATCA 
GTTACCCCAC ACGAATGGCC GGATGACATT ACAAAATGGC CGGTCTGCAC AAAAAACAGT 
GACGGACATT TCCCAAACCA TCTTCACATG GACTGTGTTA TAACAGGCAG GCCCTGTTGT 
ATTGGTACAA AAGGAAGGTG TGAAATAACT TCCAGGGAAT ATTGTGATTT TATGAAAGGA 
TACTTCCATG AAGAGGCTAC ATTGTGCTCA CAGGTGCATT GCATGGATGA TGTGTGTGGA 
CTACTGCCAT TCTTGGACCC AGAGTTTCCA GATCAGGTCT ATAGGTTATG GCTTTCTCTC 
TTCCTGCATG CAGGAGTCTT GCACTGTTTA GTTTCTGTTT GTTTTCAGAT GACAATACTG 
CGAGATTTGG AAAAGTTGGC AGGTTGGCAT AGAATATCCA TTATTTATAT ACTAAGTGGA 
ATTACTGGAA ATCTAACAAG TGCTATTTTC CTGCCATACA GGGCTGAGGT GGGGCCTGCT 
GGTTCACAGT TTGGAATTCT GGCATGCCTT TTTGTGGAAC TTTTCCAGAG CTGGCAAATT 
CTTGCAAGAC CATGGAGAGC CTTTTTCAAG CTATTTGCAG TTGTTATTTT CCTGTTCACC 
TTTGGACTCC TTCCTTGGAT TGACAATTTT GCTCATTTTG CTGGCTTTGT TAGTGGCTTC 
TTCTTATCCT TTGCATTTCT TCCTTATATT AGCTTTGGCA AATTTGATAT GTACCGTAAA 
CGATGCCAGA TTATCATATT CCTATTAATA TTTTTTGGCC TTTTTTCAGG TTTAGTAGTT 
TTGTTTTATG TGTACCCTAT CAAGTGTGAA TGGTGTGAAT ACTTAACCTG TATACCATTT 
ACAGATAAAT TCTGTGAAAA ATATGAACTT CATGCACAGC TACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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rhbdf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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