sphk2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following sphk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sphk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa13314 XM_002938279.4
Latest version!
Xenopus tropicalis sphingosine kinase 2 (sphk2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa13314 XM_004916240.3
Latest version!
Xenopus tropicalis sphingosine kinase 2 (sphk2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa62908 XM_031906420.1
Latest version!
Xenopus tropicalis sphingosine kinase 2 (sphk2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OXa62908 XM_002938279.5
Latest version!
Xenopus tropicalis sphingosine kinase 2 (sphk2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OXa62908 XM_004916240.4
Latest version!
Xenopus tropicalis sphingosine kinase 2 (sphk2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa13314
    Clone ID Related Accession (Same CDS sequence) XM_002938279.4 , XM_004916240.3
    Accession Version XM_002938279.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sphingosine kinase 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002938279.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAATATC GACAGTCATC AGAGACCCTG CTCCATGGAG AATTCGGAGT CTACCCATCC 
    AAAGGTATTC GCTATGCACT TAGCTTGACC CACACAGGTC TCCATATTCA GAGACTGGTC
    CCGAAGCCTG AAGATGACCA TCGCCTCGTT CTGCCGCTCA GTGACATGGT GGGCTGTCAC
    ACTCTGCGCA GTCACAGTTC CGGGGATATG TTTGCCTATT TTTCCATTTA TTCCTATCCG
    CTCAAGAAGA AAAAGGTCAC CATGGGACCT AGCCGCACCC GACAGAGGTT GGTTCGCACA
    TTCCGCGTAG ATGAAGAATC GGAATATAAC AGAAACCTTG CTATTGCTGA GAAGTGGGCA
    ACCGCTATAA AGTGTTTGAT TCAGGAAATT CCCATAGGCA GTGAAACAGA ATTTGTTCCT
    GAGCTTCTGC CCAGAACCCG GAGGTTAATG CTTTTGGTAA ATCCATGTGG AGGAAGAGGG
    AATGCCCTAC AGCAGTGTCA GAGCCACATA CTGCCCATGC TGACGGAGGC AGAAATCAGC
    TATAATCTCA TACAGACAGA GAGGCAGAAC CACGCGCGAG AGCTAGTGCA AGGAATCAGT
    TTGGAGCAAT GGGACGGTAT AATTGTCATC TCTGGAGACG GACTACTCTA TGAGGTTATT
    AATGGTTTGA TGGAACGCCC CGACTGGGAA GATGCAATAA AAATGCCTGT GGGAATTCTC
    CCCTGTGGCT CAGGAAATGC CTTGGCGGGA GCGATCAACT ACAATGCAGG GTTTGATCAG
    GCAATGGGGT CTGAGCTGCT GCTAAACTGC ATCTTGCTGC TGTGCCGTGG TACTGTTATT
    CCTATGGACC TGGTGTCTTT GACAACCTGC TCTGGAATCC GTTCTTTTTC ATTCCTTAGT
    GTAGCCTGGG GTTTTATCTC CGATGTGGAC ATAGAGAGCG AGAAGTACCG TCACATGGGT
    TCAGCTCGCT TCACAGTAGG AACGATGGTA AGGGTAGCCT CCCTGCGGAC TTATAGAGGC
    CGCCTTTCCT ATCTGCCAGT TTTGGATGCC CATCGCCCAA TTTGCAGGAG CATCACTTTG
    TCTCCTAATG GGAGTCTGGC TCCATTTCAG CGCTCTCAGT TGCACCGTAC CATATCAGAC
    ATTGGACTGT GCGAGGAGAG GAATGTGATG TATAAGCGCA GTTCTACTGC TCTTGACACA
    AATGAGTTGC CCAGTTTTGA GTCACCTTCT CCAAGTTCAC CTACAGCCTT GCACTCTTCC
    AGCTTCAACT TTGACTCTCT CTCGGATGAC CCTTCATATC AGGATTCTGT GTATTCCAAA
    GACTTAAAGC CTCAGAGGCA CCATCAGGTT AATGGACCCA AGGACGACCT CTTGCCTTCT
    TTGGGGCAAC CTCTGCCTAA GAACTGGATA ATTGTTGAAG ATAATTTTGT CTTAGTGTTG
    GCCATTTATC AGAGCCATTT AGGGGCTGAT TTGTTCACGG CTCCATTTTC CACTTTTGAT
    GATGGTTTGA TCCACCTTTT CTTTGTAAAA GCAGGTATAT CCCGGGCAGC CCTTGTGCGC
    CTCTTCCTAG CCATGGAAAA AGGTACACAC TTTGAGACGG AATGTCCATA CCTCACCCAC
    ATTCCTGTTA GAGCCTTCCG CTTGGAGCCT CTCACAAGAA AGGGAATTAT TACTGTAGAT
    GGAGAGCGGG TGGAATATGG GCCTATCCAG GCTCAGATAC ACAAAGGACT AAGTAACCTT
    ATCACTGGAT CTTGCAAAGT CCGTTTGACT CCATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002938325.1
    CDS658..2436
    Translation

    Target ORF information:

    RefSeq Version XM_002938279.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sphingosine kinase 2 (sphk2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002938279.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAATATC GACAGTCATC AGAGACCCTG CTCCATGGAG AATTCGGAGT CTACCCATCC 
    AAAGGTATTC GCTATGCACT TAGCTTGACC CACACAGGTC TCCATATTCA GAGACTGGTC
    CCGAAGCCTG AAGATGACCA TCGCCTCGTT CTGCCGCTCA GTGACATGGT GGGCTGTCAC
    ACTCTGCGCA GTCACAGTTC CGGGGATATG TTTGCCTATT TTTCCATTTA TTCCTATCCG
    CTCAAGAAGA AAAAGGTCAC CATGGGACCT AGCCGCACCC GACAGAGGTT GGTTCGCACA
    TTCCGCGTAG ATGAAGAATC GGAATATAAC AGAAACCTTG CTATTGCTGA GAAGTGGGCA
    ACCGCTATAA AGTGTTTGAT TCAGGAAATT CCCATAGGCA GTGAAACAGA ATTTGTTCCT
    GAGCTTCTGC CCAGAACCCG GAGGTTAATG CTTTTGGTAA ATCCATGTGG AGGAAGAGGG
    AATGCCCTAC AGCAGTGTCA GAGCCACATA CTGCCCATGC TGACGGAGGC AGAAATCAGC
    TATAATCTCA TACAGACAGA GAGGCAGAAC CACGCGCGAG AGCTAGTGCA AGGAATCAGT
    TTGGAGCAAT GGGACGGTAT AATTGTCATC TCTGGAGACG GACTACTCTA TGAGGTTATT
    AATGGTTTGA TGGAACGCCC CGACTGGGAA GATGCAATAA AAATGCCTGT GGGAATTCTC
    CCCTGTGGCT CAGGAAATGC CTTGGCGGGA GCGATCAACT ACAATGCAGG GTTTGATCAG
    GCAATGGGGT CTGAGCTGCT GCTAAACTGC ATCTTGCTGC TGTGCCGTGG TACTGTTATT
    CCTATGGACC TGGTGTCTTT GACAACCTGC TCTGGAATCC GTTCTTTTTC ATTCCTTAGT
    GTAGCCTGGG GTTTTATCTC CGATGTGGAC ATAGAGAGCG AGAAGTACCG TCACATGGGT
    TCAGCTCGCT TCACAGTAGG AACGATGGTA AGGGTAGCCT CCCTGCGGAC TTATAGAGGC
    CGCCTTTCCT ATCTGCCAGT TTTGGATGCC CATCGCCCAA TTTGCAGGAG CATCACTTTG
    TCTCCTAATG GGAGTCTGGC TCCATTTCAG CGCTCTCAGT TGCACCGTAC CATATCAGAC
    ATTGGACTGT GCGAGGAGAG GAATGTGATG TATAAGCGCA GTTCTACTGC TCTTGACACA
    AATGAGTTGC CCAGTTTTGA GTCACCTTCT CCAAGTTCAC CTACAGCCTT GCACTCTTCC
    AGCTTCAACT TTGACTCTCT CTCGGATGAC CCTTCATATC AGGATTCTGT GTATTCCAAA
    GACTTAAAGC CTCAGAGGCA CCATCAGGTT AATGGACCCA AGGACGACCT CTTGCCTTCT
    TTGGGGCAAC CTCTGCCTAA GAACTGGATA ATTGTTGAAG ATAATTTTGT CTTAGTGTTG
    GCCATTTATC AGAGCCATTT AGGGGCTGAT TTGTTCACGG CTCCATTTTC CACTTTTGAT
    GATGGTTTGA TCCACCTTTT CTTTGTAAAA GCAGGTATAT CCCGGGCAGC CCTTGTGCGC
    CTCTTCCTAG CCATGGAAAA AGGTACACAC TTTGAGACGG AATGTCCATA CCTCACCCAC
    ATTCCTGTTA GAGCCTTCCG CTTGGAGCCT CTCACAAGAA AGGGAATTAT TACTGTAGAT
    GGAGAGCGGG TGGAATATGG GCCTATCCAG GCTCAGATAC ACAAAGGACT AAGTAACCTT
    ATCACTGGAT CTTGCAAAGT CCGTTTGACT CCATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa13314
    Clone ID Related Accession (Same CDS sequence) XM_002938279.4 , XM_004916240.3
    Accession Version XM_004916240.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sphingosine kinase 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916240.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAATATC GACAGTCATC AGAGACCCTG CTCCATGGAG AATTCGGAGT CTACCCATCC 
    AAAGGTATTC GCTATGCACT TAGCTTGACC CACACAGGTC TCCATATTCA GAGACTGGTC
    CCGAAGCCTG AAGATGACCA TCGCCTCGTT CTGCCGCTCA GTGACATGGT GGGCTGTCAC
    ACTCTGCGCA GTCACAGTTC CGGGGATATG TTTGCCTATT TTTCCATTTA TTCCTATCCG
    CTCAAGAAGA AAAAGGTCAC CATGGGACCT AGCCGCACCC GACAGAGGTT GGTTCGCACA
    TTCCGCGTAG ATGAAGAATC GGAATATAAC AGAAACCTTG CTATTGCTGA GAAGTGGGCA
    ACCGCTATAA AGTGTTTGAT TCAGGAAATT CCCATAGGCA GTGAAACAGA ATTTGTTCCT
    GAGCTTCTGC CCAGAACCCG GAGGTTAATG CTTTTGGTAA ATCCATGTGG AGGAAGAGGG
    AATGCCCTAC AGCAGTGTCA GAGCCACATA CTGCCCATGC TGACGGAGGC AGAAATCAGC
    TATAATCTCA TACAGACAGA GAGGCAGAAC CACGCGCGAG AGCTAGTGCA AGGAATCAGT
    TTGGAGCAAT GGGACGGTAT AATTGTCATC TCTGGAGACG GACTACTCTA TGAGGTTATT
    AATGGTTTGA TGGAACGCCC CGACTGGGAA GATGCAATAA AAATGCCTGT GGGAATTCTC
    CCCTGTGGCT CAGGAAATGC CTTGGCGGGA GCGATCAACT ACAATGCAGG GTTTGATCAG
    GCAATGGGGT CTGAGCTGCT GCTAAACTGC ATCTTGCTGC TGTGCCGTGG TACTGTTATT
    CCTATGGACC TGGTGTCTTT GACAACCTGC TCTGGAATCC GTTCTTTTTC ATTCCTTAGT
    GTAGCCTGGG GTTTTATCTC CGATGTGGAC ATAGAGAGCG AGAAGTACCG TCACATGGGT
    TCAGCTCGCT TCACAGTAGG AACGATGGTA AGGGTAGCCT CCCTGCGGAC TTATAGAGGC
    CGCCTTTCCT ATCTGCCAGT TTTGGATGCC CATCGCCCAA TTTGCAGGAG CATCACTTTG
    TCTCCTAATG GGAGTCTGGC TCCATTTCAG CGCTCTCAGT TGCACCGTAC CATATCAGAC
    ATTGGACTGT GCGAGGAGAG GAATGTGATG TATAAGCGCA GTTCTACTGC TCTTGACACA
    AATGAGTTGC CCAGTTTTGA GTCACCTTCT CCAAGTTCAC CTACAGCCTT GCACTCTTCC
    AGCTTCAACT TTGACTCTCT CTCGGATGAC CCTTCATATC AGGATTCTGT GTATTCCAAA
    GACTTAAAGC CTCAGAGGCA CCATCAGGTT AATGGACCCA AGGACGACCT CTTGCCTTCT
    TTGGGGCAAC CTCTGCCTAA GAACTGGATA ATTGTTGAAG ATAATTTTGT CTTAGTGTTG
    GCCATTTATC AGAGCCATTT AGGGGCTGAT TTGTTCACGG CTCCATTTTC CACTTTTGAT
    GATGGTTTGA TCCACCTTTT CTTTGTAAAA GCAGGTATAT CCCGGGCAGC CCTTGTGCGC
    CTCTTCCTAG CCATGGAAAA AGGTACACAC TTTGAGACGG AATGTCCATA CCTCACCCAC
    ATTCCTGTTA GAGCCTTCCG CTTGGAGCCT CTCACAAGAA AGGGAATTAT TACTGTAGAT
    GGAGAGCGGG TGGAATATGG GCCTATCCAG GCTCAGATAC ACAAAGGACT AAGTAACCTT
    ATCACTGGAT CTTGCAAAGT CCGTTTGACT CCATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916297.1
    CDS861..2639
    Translation

    Target ORF information:

    RefSeq Version XM_004916240.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sphingosine kinase 2 (sphk2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916240.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAATATC GACAGTCATC AGAGACCCTG CTCCATGGAG AATTCGGAGT CTACCCATCC 
    AAAGGTATTC GCTATGCACT TAGCTTGACC CACACAGGTC TCCATATTCA GAGACTGGTC
    CCGAAGCCTG AAGATGACCA TCGCCTCGTT CTGCCGCTCA GTGACATGGT GGGCTGTCAC
    ACTCTGCGCA GTCACAGTTC CGGGGATATG TTTGCCTATT TTTCCATTTA TTCCTATCCG
    CTCAAGAAGA AAAAGGTCAC CATGGGACCT AGCCGCACCC GACAGAGGTT GGTTCGCACA
    TTCCGCGTAG ATGAAGAATC GGAATATAAC AGAAACCTTG CTATTGCTGA GAAGTGGGCA
    ACCGCTATAA AGTGTTTGAT TCAGGAAATT CCCATAGGCA GTGAAACAGA ATTTGTTCCT
    GAGCTTCTGC CCAGAACCCG GAGGTTAATG CTTTTGGTAA ATCCATGTGG AGGAAGAGGG
    AATGCCCTAC AGCAGTGTCA GAGCCACATA CTGCCCATGC TGACGGAGGC AGAAATCAGC
    TATAATCTCA TACAGACAGA GAGGCAGAAC CACGCGCGAG AGCTAGTGCA AGGAATCAGT
    TTGGAGCAAT GGGACGGTAT AATTGTCATC TCTGGAGACG GACTACTCTA TGAGGTTATT
    AATGGTTTGA TGGAACGCCC CGACTGGGAA GATGCAATAA AAATGCCTGT GGGAATTCTC
    CCCTGTGGCT CAGGAAATGC CTTGGCGGGA GCGATCAACT ACAATGCAGG GTTTGATCAG
    GCAATGGGGT CTGAGCTGCT GCTAAACTGC ATCTTGCTGC TGTGCCGTGG TACTGTTATT
    CCTATGGACC TGGTGTCTTT GACAACCTGC TCTGGAATCC GTTCTTTTTC ATTCCTTAGT
    GTAGCCTGGG GTTTTATCTC CGATGTGGAC ATAGAGAGCG AGAAGTACCG TCACATGGGT
    TCAGCTCGCT TCACAGTAGG AACGATGGTA AGGGTAGCCT CCCTGCGGAC TTATAGAGGC
    CGCCTTTCCT ATCTGCCAGT TTTGGATGCC CATCGCCCAA TTTGCAGGAG CATCACTTTG
    TCTCCTAATG GGAGTCTGGC TCCATTTCAG CGCTCTCAGT TGCACCGTAC CATATCAGAC
    ATTGGACTGT GCGAGGAGAG GAATGTGATG TATAAGCGCA GTTCTACTGC TCTTGACACA
    AATGAGTTGC CCAGTTTTGA GTCACCTTCT CCAAGTTCAC CTACAGCCTT GCACTCTTCC
    AGCTTCAACT TTGACTCTCT CTCGGATGAC CCTTCATATC AGGATTCTGT GTATTCCAAA
    GACTTAAAGC CTCAGAGGCA CCATCAGGTT AATGGACCCA AGGACGACCT CTTGCCTTCT
    TTGGGGCAAC CTCTGCCTAA GAACTGGATA ATTGTTGAAG ATAATTTTGT CTTAGTGTTG
    GCCATTTATC AGAGCCATTT AGGGGCTGAT TTGTTCACGG CTCCATTTTC CACTTTTGAT
    GATGGTTTGA TCCACCTTTT CTTTGTAAAA GCAGGTATAT CCCGGGCAGC CCTTGTGCGC
    CTCTTCCTAG CCATGGAAAA AGGTACACAC TTTGAGACGG AATGTCCATA CCTCACCCAC
    ATTCCTGTTA GAGCCTTCCG CTTGGAGCCT CTCACAAGAA AGGGAATTAT TACTGTAGAT
    GGAGAGCGGG TGGAATATGG GCCTATCCAG GCTCAGATAC ACAAAGGACT AAGTAACCTT
    ATCACTGGAT CTTGCAAAGT CCGTTTGACT CCATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62908
    Clone ID Related Accession (Same CDS sequence) XM_002938279.5 , XM_031906420.1 , XM_004916240.4
    Accession Version XM_002938279.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sphingosine kinase 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002938279.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAATATC GACAGTCATC AGAGACCCTG CTCCATGGAG AATTCGGAGT CTACCCATCC 
    AAAGGTATTC GCTATGCACT TAGCTTGACC CACACAGGTC TCCATATCCA GAGACTGGTC
    CCGAAGCCTG AAGATGACCA TCGCCTCGTT CTGCCGCTCA GTGACATGGT GGGCTGTCAC
    ACTCTGCGCA GTCACAGTTC CGGGGATATG TTTGCCTATT TTTCCATTTA TTCCTATCCG
    CTCAAGAAGA AAAAGGTCAC CATGGGACCT AGCCGCACCC GACAGAGGTT GGTTCGCACA
    TTCCGCGTAG ATGAAGAATC GGAATATAAC AGAAACCTTG CTATTGCTGA GAAGTGGGCA
    ACCGCTATAA AGTGTTTGAT TCAGGAAATT CCCATAGGCA GTGAAACAGA ATTTGTTCCT
    GAGCTTCTGC CCAGAACCCG GAGGTTAATG CTTTTGGTAA ATCCATGTGG AGGAAGAGGG
    AATGCCCTCC AGCAGTGTCA GAGCCACATA CTGCCCATGC TGACGGAGGC AGAAATCAGC
    TATAATCTCA TACAGACAGA GAGGCAGAAC CACGCGCGAG AGCTAGTGCA AGGAATCAGT
    TTGGAGCAAT GGGACGGTAT AATTGTCATC TCTGGAGACG GACTACTCTA TGAGGTTATT
    AATGGTTTGA TGGAACGCCC CGACTGGGAA GATGCAATAA AAATGCCTGT GGGAATTCTC
    CCCTGTGGCT CAGGAAATGC CTTGGCGGGA GCGATCAACT ACAATGCAGG GTTTGATCAG
    GCAATGGGGT CTGAGCTGCT GCTAAACTGC ATCTTGCTGC TGTGCCGTGG TACTGTTATT
    CCTATGGACC TGGTGTCTTT GACAACCTGC TCTGGAATCC GTTCTTTTTC ATTCCTTAGT
    GTAGCCTGGG GTTTTATCTC CGATGTGGAC ATAGAGAGCG AGAAGTACCG TCACATGGGT
    TCAGCTCGCT TCACAGTAGG AACGATGGTA AGGGTAGCCT CCCTGCGGAC TTATAGAGGC
    CGCCTTTCCT ATCTGCCAGT TTTGGATGCC CATCGCCCAA TTTGCAGGAG CATCACTTTG
    TCTCCTAATG GGAGTCTGGC TCCATTTCAG CGCTCTCAGT TGCACCGTAC CATATCAGAC
    ATTGGACTGT GCGAGGAGAG GAATGTGATG TATAAGCGCA GTTCTACTGC TCTTGACACA
    AATGAGTTGC CCAGTTTTGA GTCACCTTCT CCAAGTTCAC CTACAGCCTT GCACTCTTCC
    AGCTTCAACT TTGACTCTCT CTCGGATGAC CCTTCATATC AGGATTCTGT GTATTCCAAA
    GACTTAAAGC CTCAGAGGCA CCATCAGGTT AATGGACCCA AGGACGACCT CTTGCCTTCT
    TTGGGGCAAC CTCTGCCTAA GAACTGGATA ATTGTTGAAG ATAATTTTGT CTTAGTGTTG
    GCCATTTATC AGAGCCATTT AGGGGCTGAT TTGTTCACGG CTCCATTTTC CACTTTTGAT
    GATGGTTTGA TCCACCTTTT CTTTGTAAAA GCAGGTATAT CCCGGGCAGC CCTTGTGCGC
    CTCTTCCTAG CCATGGAAAA AGGTACACAC TTTGAGACGG AATGTCCATA CCTCACCCAC
    ATTCCTGTTA GAGCCTTCCG CTTGGAGCCT CTCACAAGAA AGGGAATTAT TACTGTAGAT
    GGAGAGCGGG TGGAATATGG GCCTATCCAG GCTCAGATAC ACAAAGGACT AAGTAACCTT
    ATCACTGGAT CTTGCAAAGT CCGTTTGACT CCATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002938325.1
    CDS563..2341
    Translation

    Target ORF information:

    RefSeq Version XM_002938279.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sphingosine kinase 2 (sphk2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002938279.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAATATC GACAGTCATC AGAGACCCTG CTCCATGGAG AATTCGGAGT CTACCCATCC 
    AAAGGTATTC GCTATGCACT TAGCTTGACC CACACAGGTC TCCATATCCA GAGACTGGTC
    CCGAAGCCTG AAGATGACCA TCGCCTCGTT CTGCCGCTCA GTGACATGGT GGGCTGTCAC
    ACTCTGCGCA GTCACAGTTC CGGGGATATG TTTGCCTATT TTTCCATTTA TTCCTATCCG
    CTCAAGAAGA AAAAGGTCAC CATGGGACCT AGCCGCACCC GACAGAGGTT GGTTCGCACA
    TTCCGCGTAG ATGAAGAATC GGAATATAAC AGAAACCTTG CTATTGCTGA GAAGTGGGCA
    ACCGCTATAA AGTGTTTGAT TCAGGAAATT CCCATAGGCA GTGAAACAGA ATTTGTTCCT
    GAGCTTCTGC CCAGAACCCG GAGGTTAATG CTTTTGGTAA ATCCATGTGG AGGAAGAGGG
    AATGCCCTCC AGCAGTGTCA GAGCCACATA CTGCCCATGC TGACGGAGGC AGAAATCAGC
    TATAATCTCA TACAGACAGA GAGGCAGAAC CACGCGCGAG AGCTAGTGCA AGGAATCAGT
    TTGGAGCAAT GGGACGGTAT AATTGTCATC TCTGGAGACG GACTACTCTA TGAGGTTATT
    AATGGTTTGA TGGAACGCCC CGACTGGGAA GATGCAATAA AAATGCCTGT GGGAATTCTC
    CCCTGTGGCT CAGGAAATGC CTTGGCGGGA GCGATCAACT ACAATGCAGG GTTTGATCAG
    GCAATGGGGT CTGAGCTGCT GCTAAACTGC ATCTTGCTGC TGTGCCGTGG TACTGTTATT
    CCTATGGACC TGGTGTCTTT GACAACCTGC TCTGGAATCC GTTCTTTTTC ATTCCTTAGT
    GTAGCCTGGG GTTTTATCTC CGATGTGGAC ATAGAGAGCG AGAAGTACCG TCACATGGGT
    TCAGCTCGCT TCACAGTAGG AACGATGGTA AGGGTAGCCT CCCTGCGGAC TTATAGAGGC
    CGCCTTTCCT ATCTGCCAGT TTTGGATGCC CATCGCCCAA TTTGCAGGAG CATCACTTTG
    TCTCCTAATG GGAGTCTGGC TCCATTTCAG CGCTCTCAGT TGCACCGTAC CATATCAGAC
    ATTGGACTGT GCGAGGAGAG GAATGTGATG TATAAGCGCA GTTCTACTGC TCTTGACACA
    AATGAGTTGC CCAGTTTTGA GTCACCTTCT CCAAGTTCAC CTACAGCCTT GCACTCTTCC
    AGCTTCAACT TTGACTCTCT CTCGGATGAC CCTTCATATC AGGATTCTGT GTATTCCAAA
    GACTTAAAGC CTCAGAGGCA CCATCAGGTT AATGGACCCA AGGACGACCT CTTGCCTTCT
    TTGGGGCAAC CTCTGCCTAA GAACTGGATA ATTGTTGAAG ATAATTTTGT CTTAGTGTTG
    GCCATTTATC AGAGCCATTT AGGGGCTGAT TTGTTCACGG CTCCATTTTC CACTTTTGAT
    GATGGTTTGA TCCACCTTTT CTTTGTAAAA GCAGGTATAT CCCGGGCAGC CCTTGTGCGC
    CTCTTCCTAG CCATGGAAAA AGGTACACAC TTTGAGACGG AATGTCCATA CCTCACCCAC
    ATTCCTGTTA GAGCCTTCCG CTTGGAGCCT CTCACAAGAA AGGGAATTAT TACTGTAGAT
    GGAGAGCGGG TGGAATATGG GCCTATCCAG GCTCAGATAC ACAAAGGACT AAGTAACCTT
    ATCACTGGAT CTTGCAAAGT CCGTTTGACT CCATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62908
    Clone ID Related Accession (Same CDS sequence) XM_002938279.5 , XM_031906420.1 , XM_004916240.4
    Accession Version XM_031906420.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sphingosine kinase 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAATATC GACAGTCATC AGAGACCCTG CTCCATGGAG AATTCGGAGT CTACCCATCC 
    AAAGGTATTC GCTATGCACT TAGCTTGACC CACACAGGTC TCCATATCCA GAGACTGGTC
    CCGAAGCCTG AAGATGACCA TCGCCTCGTT CTGCCGCTCA GTGACATGGT GGGCTGTCAC
    ACTCTGCGCA GTCACAGTTC CGGGGATATG TTTGCCTATT TTTCCATTTA TTCCTATCCG
    CTCAAGAAGA AAAAGGTCAC CATGGGACCT AGCCGCACCC GACAGAGGTT GGTTCGCACA
    TTCCGCGTAG ATGAAGAATC GGAATATAAC AGAAACCTTG CTATTGCTGA GAAGTGGGCA
    ACCGCTATAA AGTGTTTGAT TCAGGAAATT CCCATAGGCA GTGAAACAGA ATTTGTTCCT
    GAGCTTCTGC CCAGAACCCG GAGGTTAATG CTTTTGGTAA ATCCATGTGG AGGAAGAGGG
    AATGCCCTCC AGCAGTGTCA GAGCCACATA CTGCCCATGC TGACGGAGGC AGAAATCAGC
    TATAATCTCA TACAGACAGA GAGGCAGAAC CACGCGCGAG AGCTAGTGCA AGGAATCAGT
    TTGGAGCAAT GGGACGGTAT AATTGTCATC TCTGGAGACG GACTACTCTA TGAGGTTATT
    AATGGTTTGA TGGAACGCCC CGACTGGGAA GATGCAATAA AAATGCCTGT GGGAATTCTC
    CCCTGTGGCT CAGGAAATGC CTTGGCGGGA GCGATCAACT ACAATGCAGG GTTTGATCAG
    GCAATGGGGT CTGAGCTGCT GCTAAACTGC ATCTTGCTGC TGTGCCGTGG TACTGTTATT
    CCTATGGACC TGGTGTCTTT GACAACCTGC TCTGGAATCC GTTCTTTTTC ATTCCTTAGT
    GTAGCCTGGG GTTTTATCTC CGATGTGGAC ATAGAGAGCG AGAAGTACCG TCACATGGGT
    TCAGCTCGCT TCACAGTAGG AACGATGGTA AGGGTAGCCT CCCTGCGGAC TTATAGAGGC
    CGCCTTTCCT ATCTGCCAGT TTTGGATGCC CATCGCCCAA TTTGCAGGAG CATCACTTTG
    TCTCCTAATG GGAGTCTGGC TCCATTTCAG CGCTCTCAGT TGCACCGTAC CATATCAGAC
    ATTGGACTGT GCGAGGAGAG GAATGTGATG TATAAGCGCA GTTCTACTGC TCTTGACACA
    AATGAGTTGC CCAGTTTTGA GTCACCTTCT CCAAGTTCAC CTACAGCCTT GCACTCTTCC
    AGCTTCAACT TTGACTCTCT CTCGGATGAC CCTTCATATC AGGATTCTGT GTATTCCAAA
    GACTTAAAGC CTCAGAGGCA CCATCAGGTT AATGGACCCA AGGACGACCT CTTGCCTTCT
    TTGGGGCAAC CTCTGCCTAA GAACTGGATA ATTGTTGAAG ATAATTTTGT CTTAGTGTTG
    GCCATTTATC AGAGCCATTT AGGGGCTGAT TTGTTCACGG CTCCATTTTC CACTTTTGAT
    GATGGTTTGA TCCACCTTTT CTTTGTAAAA GCAGGTATAT CCCGGGCAGC CCTTGTGCGC
    CTCTTCCTAG CCATGGAAAA AGGTACACAC TTTGAGACGG AATGTCCATA CCTCACCCAC
    ATTCCTGTTA GAGCCTTCCG CTTGGAGCCT CTCACAAGAA AGGGAATTAT TACTGTAGAT
    GGAGAGCGGG TGGAATATGG GCCTATCCAG GCTCAGATAC ACAAAGGACT AAGTAACCTT
    ATCACTGGAT CTTGCAAAGT CCGTTTGACT CCATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031762280.1
    CDS493..2271
    Translation

    Target ORF information:

    RefSeq Version XM_031906420.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sphingosine kinase 2 (sphk2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906420.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAATATC GACAGTCATC AGAGACCCTG CTCCATGGAG AATTCGGAGT CTACCCATCC 
    AAAGGTATTC GCTATGCACT TAGCTTGACC CACACAGGTC TCCATATCCA GAGACTGGTC
    CCGAAGCCTG AAGATGACCA TCGCCTCGTT CTGCCGCTCA GTGACATGGT GGGCTGTCAC
    ACTCTGCGCA GTCACAGTTC CGGGGATATG TTTGCCTATT TTTCCATTTA TTCCTATCCG
    CTCAAGAAGA AAAAGGTCAC CATGGGACCT AGCCGCACCC GACAGAGGTT GGTTCGCACA
    TTCCGCGTAG ATGAAGAATC GGAATATAAC AGAAACCTTG CTATTGCTGA GAAGTGGGCA
    ACCGCTATAA AGTGTTTGAT TCAGGAAATT CCCATAGGCA GTGAAACAGA ATTTGTTCCT
    GAGCTTCTGC CCAGAACCCG GAGGTTAATG CTTTTGGTAA ATCCATGTGG AGGAAGAGGG
    AATGCCCTCC AGCAGTGTCA GAGCCACATA CTGCCCATGC TGACGGAGGC AGAAATCAGC
    TATAATCTCA TACAGACAGA GAGGCAGAAC CACGCGCGAG AGCTAGTGCA AGGAATCAGT
    TTGGAGCAAT GGGACGGTAT AATTGTCATC TCTGGAGACG GACTACTCTA TGAGGTTATT
    AATGGTTTGA TGGAACGCCC CGACTGGGAA GATGCAATAA AAATGCCTGT GGGAATTCTC
    CCCTGTGGCT CAGGAAATGC CTTGGCGGGA GCGATCAACT ACAATGCAGG GTTTGATCAG
    GCAATGGGGT CTGAGCTGCT GCTAAACTGC ATCTTGCTGC TGTGCCGTGG TACTGTTATT
    CCTATGGACC TGGTGTCTTT GACAACCTGC TCTGGAATCC GTTCTTTTTC ATTCCTTAGT
    GTAGCCTGGG GTTTTATCTC CGATGTGGAC ATAGAGAGCG AGAAGTACCG TCACATGGGT
    TCAGCTCGCT TCACAGTAGG AACGATGGTA AGGGTAGCCT CCCTGCGGAC TTATAGAGGC
    CGCCTTTCCT ATCTGCCAGT TTTGGATGCC CATCGCCCAA TTTGCAGGAG CATCACTTTG
    TCTCCTAATG GGAGTCTGGC TCCATTTCAG CGCTCTCAGT TGCACCGTAC CATATCAGAC
    ATTGGACTGT GCGAGGAGAG GAATGTGATG TATAAGCGCA GTTCTACTGC TCTTGACACA
    AATGAGTTGC CCAGTTTTGA GTCACCTTCT CCAAGTTCAC CTACAGCCTT GCACTCTTCC
    AGCTTCAACT TTGACTCTCT CTCGGATGAC CCTTCATATC AGGATTCTGT GTATTCCAAA
    GACTTAAAGC CTCAGAGGCA CCATCAGGTT AATGGACCCA AGGACGACCT CTTGCCTTCT
    TTGGGGCAAC CTCTGCCTAA GAACTGGATA ATTGTTGAAG ATAATTTTGT CTTAGTGTTG
    GCCATTTATC AGAGCCATTT AGGGGCTGAT TTGTTCACGG CTCCATTTTC CACTTTTGAT
    GATGGTTTGA TCCACCTTTT CTTTGTAAAA GCAGGTATAT CCCGGGCAGC CCTTGTGCGC
    CTCTTCCTAG CCATGGAAAA AGGTACACAC TTTGAGACGG AATGTCCATA CCTCACCCAC
    ATTCCTGTTA GAGCCTTCCG CTTGGAGCCT CTCACAAGAA AGGGAATTAT TACTGTAGAT
    GGAGAGCGGG TGGAATATGG GCCTATCCAG GCTCAGATAC ACAAAGGACT AAGTAACCTT
    ATCACTGGAT CTTGCAAAGT CCGTTTGACT CCATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62908
    Clone ID Related Accession (Same CDS sequence) XM_002938279.5 , XM_031906420.1 , XM_004916240.4
    Accession Version XM_004916240.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1779bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product sphingosine kinase 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916240.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAATATC GACAGTCATC AGAGACCCTG CTCCATGGAG AATTCGGAGT CTACCCATCC 
    AAAGGTATTC GCTATGCACT TAGCTTGACC CACACAGGTC TCCATATCCA GAGACTGGTC
    CCGAAGCCTG AAGATGACCA TCGCCTCGTT CTGCCGCTCA GTGACATGGT GGGCTGTCAC
    ACTCTGCGCA GTCACAGTTC CGGGGATATG TTTGCCTATT TTTCCATTTA TTCCTATCCG
    CTCAAGAAGA AAAAGGTCAC CATGGGACCT AGCCGCACCC GACAGAGGTT GGTTCGCACA
    TTCCGCGTAG ATGAAGAATC GGAATATAAC AGAAACCTTG CTATTGCTGA GAAGTGGGCA
    ACCGCTATAA AGTGTTTGAT TCAGGAAATT CCCATAGGCA GTGAAACAGA ATTTGTTCCT
    GAGCTTCTGC CCAGAACCCG GAGGTTAATG CTTTTGGTAA ATCCATGTGG AGGAAGAGGG
    AATGCCCTCC AGCAGTGTCA GAGCCACATA CTGCCCATGC TGACGGAGGC AGAAATCAGC
    TATAATCTCA TACAGACAGA GAGGCAGAAC CACGCGCGAG AGCTAGTGCA AGGAATCAGT
    TTGGAGCAAT GGGACGGTAT AATTGTCATC TCTGGAGACG GACTACTCTA TGAGGTTATT
    AATGGTTTGA TGGAACGCCC CGACTGGGAA GATGCAATAA AAATGCCTGT GGGAATTCTC
    CCCTGTGGCT CAGGAAATGC CTTGGCGGGA GCGATCAACT ACAATGCAGG GTTTGATCAG
    GCAATGGGGT CTGAGCTGCT GCTAAACTGC ATCTTGCTGC TGTGCCGTGG TACTGTTATT
    CCTATGGACC TGGTGTCTTT GACAACCTGC TCTGGAATCC GTTCTTTTTC ATTCCTTAGT
    GTAGCCTGGG GTTTTATCTC CGATGTGGAC ATAGAGAGCG AGAAGTACCG TCACATGGGT
    TCAGCTCGCT TCACAGTAGG AACGATGGTA AGGGTAGCCT CCCTGCGGAC TTATAGAGGC
    CGCCTTTCCT ATCTGCCAGT TTTGGATGCC CATCGCCCAA TTTGCAGGAG CATCACTTTG
    TCTCCTAATG GGAGTCTGGC TCCATTTCAG CGCTCTCAGT TGCACCGTAC CATATCAGAC
    ATTGGACTGT GCGAGGAGAG GAATGTGATG TATAAGCGCA GTTCTACTGC TCTTGACACA
    AATGAGTTGC CCAGTTTTGA GTCACCTTCT CCAAGTTCAC CTACAGCCTT GCACTCTTCC
    AGCTTCAACT TTGACTCTCT CTCGGATGAC CCTTCATATC AGGATTCTGT GTATTCCAAA
    GACTTAAAGC CTCAGAGGCA CCATCAGGTT AATGGACCCA AGGACGACCT CTTGCCTTCT
    TTGGGGCAAC CTCTGCCTAA GAACTGGATA ATTGTTGAAG ATAATTTTGT CTTAGTGTTG
    GCCATTTATC AGAGCCATTT AGGGGCTGAT TTGTTCACGG CTCCATTTTC CACTTTTGAT
    GATGGTTTGA TCCACCTTTT CTTTGTAAAA GCAGGTATAT CCCGGGCAGC CCTTGTGCGC
    CTCTTCCTAG CCATGGAAAA AGGTACACAC TTTGAGACGG AATGTCCATA CCTCACCCAC
    ATTCCTGTTA GAGCCTTCCG CTTGGAGCCT CTCACAAGAA AGGGAATTAT TACTGTAGAT
    GGAGAGCGGG TGGAATATGG GCCTATCCAG GCTCAGATAC ACAAAGGACT AAGTAACCTT
    ATCACTGGAT CTTGCAAAGT CCGTTTGACT CCATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916297.1
    CDS766..2544
    Translation

    Target ORF information:

    RefSeq Version XM_004916240.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis sphingosine kinase 2 (sphk2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916240.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGAATATC GACAGTCATC AGAGACCCTG CTCCATGGAG AATTCGGAGT CTACCCATCC 
    AAAGGTATTC GCTATGCACT TAGCTTGACC CACACAGGTC TCCATATCCA GAGACTGGTC
    CCGAAGCCTG AAGATGACCA TCGCCTCGTT CTGCCGCTCA GTGACATGGT GGGCTGTCAC
    ACTCTGCGCA GTCACAGTTC CGGGGATATG TTTGCCTATT TTTCCATTTA TTCCTATCCG
    CTCAAGAAGA AAAAGGTCAC CATGGGACCT AGCCGCACCC GACAGAGGTT GGTTCGCACA
    TTCCGCGTAG ATGAAGAATC GGAATATAAC AGAAACCTTG CTATTGCTGA GAAGTGGGCA
    ACCGCTATAA AGTGTTTGAT TCAGGAAATT CCCATAGGCA GTGAAACAGA ATTTGTTCCT
    GAGCTTCTGC CCAGAACCCG GAGGTTAATG CTTTTGGTAA ATCCATGTGG AGGAAGAGGG
    AATGCCCTCC AGCAGTGTCA GAGCCACATA CTGCCCATGC TGACGGAGGC AGAAATCAGC
    TATAATCTCA TACAGACAGA GAGGCAGAAC CACGCGCGAG AGCTAGTGCA AGGAATCAGT
    TTGGAGCAAT GGGACGGTAT AATTGTCATC TCTGGAGACG GACTACTCTA TGAGGTTATT
    AATGGTTTGA TGGAACGCCC CGACTGGGAA GATGCAATAA AAATGCCTGT GGGAATTCTC
    CCCTGTGGCT CAGGAAATGC CTTGGCGGGA GCGATCAACT ACAATGCAGG GTTTGATCAG
    GCAATGGGGT CTGAGCTGCT GCTAAACTGC ATCTTGCTGC TGTGCCGTGG TACTGTTATT
    CCTATGGACC TGGTGTCTTT GACAACCTGC TCTGGAATCC GTTCTTTTTC ATTCCTTAGT
    GTAGCCTGGG GTTTTATCTC CGATGTGGAC ATAGAGAGCG AGAAGTACCG TCACATGGGT
    TCAGCTCGCT TCACAGTAGG AACGATGGTA AGGGTAGCCT CCCTGCGGAC TTATAGAGGC
    CGCCTTTCCT ATCTGCCAGT TTTGGATGCC CATCGCCCAA TTTGCAGGAG CATCACTTTG
    TCTCCTAATG GGAGTCTGGC TCCATTTCAG CGCTCTCAGT TGCACCGTAC CATATCAGAC
    ATTGGACTGT GCGAGGAGAG GAATGTGATG TATAAGCGCA GTTCTACTGC TCTTGACACA
    AATGAGTTGC CCAGTTTTGA GTCACCTTCT CCAAGTTCAC CTACAGCCTT GCACTCTTCC
    AGCTTCAACT TTGACTCTCT CTCGGATGAC CCTTCATATC AGGATTCTGT GTATTCCAAA
    GACTTAAAGC CTCAGAGGCA CCATCAGGTT AATGGACCCA AGGACGACCT CTTGCCTTCT
    TTGGGGCAAC CTCTGCCTAA GAACTGGATA ATTGTTGAAG ATAATTTTGT CTTAGTGTTG
    GCCATTTATC AGAGCCATTT AGGGGCTGAT TTGTTCACGG CTCCATTTTC CACTTTTGAT
    GATGGTTTGA TCCACCTTTT CTTTGTAAAA GCAGGTATAT CCCGGGCAGC CCTTGTGCGC
    CTCTTCCTAG CCATGGAAAA AGGTACACAC TTTGAGACGG AATGTCCATA CCTCACCCAC
    ATTCCTGTTA GAGCCTTCCG CTTGGAGCCT CTCACAAGAA AGGGAATTAT TACTGTAGAT
    GGAGAGCGGG TGGAATATGG GCCTATCCAG GCTCAGATAC ACAAAGGACT AAGTAACCTT
    ATCACTGGAT CTTGCAAAGT CCGTTTGACT CCATTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.