carf cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	carf
Entrez Gene ID	100494660
Full Name	calcium responsive transcription factor
General protein information	Preferred Names calcium responsive transcription factor Names LOW QUALITY PROTEIN: calcium-responsive transcription factor
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031892862.1	XP_031748722.1	calcium-responsive transcription factor isoform X1
XM_012971179.3	XP_012826633.3	calcium-responsive transcription factor isoform X2
XM_031892863.1	XP_031748723.1	calcium-responsive transcription factor isoform X2
XM_031892861.1	XP_031748721.1	calcium-responsive transcription factor isoform X1
XM_012971179.2	XP_012826633.2	LOW QUALITY PROTEIN: calcium-responsive transcription factor
XM_031892860.1	XP_031748720.1	calcium-responsive transcription factor isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ALS2CR8	XP_005640611.1
Rattus norvegicus (Norway rat)	Carf	NP_001100385.1
Gallus gallus (chicken)	ALS2CR8	XP_421946.3
Homo sapiens (human)	CARF	NP_079020.13
Pan troglodytes (chimpanzee)	ALS2CR8	XP_001173024.1
	LOC704415	XP_002808065.1
Danio rerio (zebrafish)	carf	XP_005167555.1
Xenopus tropicalis (tropical clawed frog)	carf	XP_002937763.2
Bos taurus (cattle)	CARF	NP_001027465.1
Mus musculus (house mouse)	Carf	NP_631889.1

Gene Ontology

The following carf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the carf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa66243	XM_031892861.1 Latest version!	Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa66243	XM_031892862.1 Latest version!	Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa66243	XM_031892860.1 Latest version!	Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa66244	XM_012971179.3 Latest version!	Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa66244	XM_031892863.1 Latest version!	Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa46879	XM_012971179.2 Latest version!	Xenopus tropicalis calcium responsive transcription factor (carf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

carf ( XM_031892862.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748722.1 Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa66243
Clone ID Related Accession (Same CDS sequence)	XM_031892862.1 , XM_031892861.1 , XM_031892860.1
Accession Version	XM_031892862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calcium-responsive transcription factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTA ATGGACTGGA CGTTGATGTA CTGACTCGGC CTGCAGACTT GTTGATCACT 
TGTACCAATA CAGAGGATAA TGCTGATCTG GATGATGGGG TTCTCAAGAA TACTGCAAAC 
CAAATATCGA CCCTCACTGA GCAGTACCAT TTTATAGATC AAAGTGGAAA TACCATCCAC 
TATGCAATTC AGGCAGTGCC TGGTGCCAAC CCCCAAATGG ATTTTATAGT AACTTCCTTT 
TGCCATTTGT TGTTGAAGAT GATCCTTACC AGTGAATCAG AAAATGGGCA AGTGTTCCAT 
GTCATTCCTT CCAGTCAGTC AGGAACAACT CAGTTTGTTA TTCCACAGGG GCAGGACATA 
GATGAGCAGC TGCAACAGAA TGAAAGCACT GAAAATCTGC AGGTAGTAGC AGGCAACACT 
GTGACAGAAA ATGCAGCCAG CTTTATTATT CATTCCAATG CATCAATGGC CATATCAAAG 
ATACCATCTG AGAGGTCACC TACAGAGTTG TTCCAAAAAC CTCTCCTACC ACTGCCAACA 
AACATTCCTG TTTGGGCTCG TCGTCTCAGG AACTGTGAGA AAATAGGAGA CTCTTACCGT 
GGTTACTGCT TAAGTGAGAA TGAGCTAATG AGCATTTTAG CACTACACAA ACAGCAAACA 
CAGAGTGTTT GGGGAACCCG CCAGTCTCCT AGTCCTGCTA AACCAGCTAC AAGACTTATG 
TGGAAGTCAC AGTATGTTCC ATATGACGGA ATTCCTTTTG TTAATGCAGG GAGCAGAGCA 
ATCGTTATGG AATGTCAGTA TGGGCCACGA AGAAAAGGAG CCCAACCCAA AAAGAGCGCT 
GAGAATGACA ACATGTCTGG GGGACTGTAC AAAGCAACTT GTCCTGCAAG GATATACATC 
AAAAAAGTAA AGAAGTTCCC TAATTACAGA GTGCCCACAG ATCCAAAGAT TGACAAGAAA 
CTTATAAGGA TGGAACAGGA GAAAGCCTTT AATTTGTTAA AGAAGGATCT GGATGGGACA 
GATGGAGTTC TCAGGTGGTA CATGCAGTTA CCAGATCAAG AAGCTCATCA GTATCATGAT 
CTGGAAACTG GTTGCTTTTC ACCATCAACT TTCCAGCCAT CCATTGAAGA TGAGGAGGAG 
ATTCTGTGTG ACGAGAACTC ACAACCCTCA CGGCTACATC CACAAGTAGC AGAAAAAATC 
CGTGATCTGG TGTCCAAAGG TTTGAGTGAG GTTTACTCAG TTAGGAAACA GCTTAGGAAA 
TTTGTGGAGC GGGATCTGTT TAAACCCGAT GACATACCTG AGCGCCATAA CTTATCCTAC 
TTTCCAACTG TGAATGATCT AAAAAATCAT ATTCATGAGG CACAGAAATC TTTGGCAAAC 
GAGGAGCTCA TTTATGATGC GGAAACAATC CCTGAGCCAG TTAGAGAGAT CCAGTGGACT 
GCTGACAGTG GAAACGTTCT TAATGAGATG GTTACTGTAA CATTTGCACC AATTCAACAG 
GAAGAACAAG AGACATTACT GAAAGTGGAG AATCCTCCCT CAGAGGATGT TTGCCAAGAC 
ACAGCTCAGC TGATCTCCTC CCTGACCTCA CTTCAGCCCA AAATCTTTGC TCAGCTACAG 
GGACTCCAGC TGCAACCGTC CTTAACATCA GTAGATGGAA AAACTGCTCT TATCACTGTG 
AATCCACCAG GTCCAACCTT TCTACCGGCT GAAGGTAAAA CTGCACTTAT TACTGTTAAT 
CCCGTGGACC AATCATTTAC AACTTCTGAT GGGAAAACTG CACTTTTTAC TGTAAATCCC 
CCTGCTCCTT CATTTACATC TGCTGATGGA ACTGCTGCAC TTATCACTGT GAATTCTCCT 
GCTCCTTCAT TTACATCAGC GGATGGGACA GCGGCATTGA TCACTGTGCA TCCCTCTGCC 
ATAGGCAGTT CTACATGCCT GTTGGGATCA GGAGGAAACA GTCAGCCTTT GGTGGTCAGT 
CATTTGCAGA ATGACTCCCC TGTGTCCTCT GTCACTAGTT TACCTGCCTC TGCCCACAAC 
CTAGTCAGTA TGAGCCAGAT TGTAACAGTG GAAGAGGCAA GTGGAATGGC TGGAGAGGTG 
CATCAAATTC TGCTGGGAGA TATCCACCCC ATTCCTATCA GGATAGTGGA GAACCAACAA 
ACAATTGTTG AAATAAATGC TCCTAATGCA TTTAGCACAG AGGATGTTGT AGCATTAAAT 
AAAGAGGTGA CTGAAGTACA GTTGAGTGCT ACAGAAGATA GTGCCACAGA TCCACAAACC 
CCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748722.1
CDS	670..2958
Translation	MESNGLDVDVLTRPADLLITCTNTEDNADLDDGVLKNTANQISTLTEQYHFIDQSGNTIHYAIQAVPGANPQMDFIVTSFCHLLLKMILTSESENGQVFHVIPSSQSGTTQFVIPQGQDIDEQLQQNESTENLQVVAGNTVTENAASFIIHSNASMAISKIPSERSPTELFQKPLLPLPTNIPVWARRLRNCEKIGDSYRGYCLSENELMSILALHKQQTQSVWGTRQSPSPAKPATRLMWKSQYVPYDGIPFVNAGSRAIVMECQYGPRRKGAQPKKSAENDNMSGGLYKATCPARIYIKKVKKFPNYRVPTDPKIDKKLIRMEQEKAFNLLKKDLDGTDGVLRWYMQLPDQEAHQYHDLETGCFSPSTFQPSIEDEEEILCDENSQPSRLHPQVAEKIRDLVSKGLSEVYSVRKQLRKFVERDLFKPDDIPERHNLSYFPTVNDLKNHIHEAQKSLANEELIYDAETIPEPVREIQWTADSGNVLNEMVTVTFAPIQQEEQETLLKVENPPSEDVCQDTAQLISSLTSLQPKIFAQLQGLQLQPSLTSVDGKTALITVNPPGPTFLPAEGKTALITVNPVDQSFTTSDGKTALFTVNPPAPSFTSADGTAALITVNSPAPSFTSADGTAALITVHPSAIGSSTCLLGSGGNSQPLVVSHLQNDSPVSSVTSLPASAHNLVSMSQIVTVEEASGMAGEVHQILLGDIHPIPIRIVENQQTIVEINAPNAFSTEDVVALNKEVTEVQLSATEDSATDPQTPV

Target ORF information:

RefSeq Version	XM_031892862.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892862.1

ORF Insert Sequence:

ATGGAAAGTA ATGGACTGGA CGTTGATGTA CTGACTCGGC CTGCAGACTT GTTGATCACT 
TGTACCAATA CAGAGGATAA TGCTGATCTG GATGATGGGG TTCTCAAGAA TACTGCAAAC 
CAAATATCGA CCCTCACTGA GCAGTACCAT TTTATAGATC AAAGTGGAAA TACCATCCAC 
TATGCAATTC AGGCAGTGCC TGGTGCCAAC CCCCAAATGG ATTTTATAGT AACTTCCTTT 
TGCCATTTGT TGTTGAAGAT GATCCTTACC AGTGAATCAG AAAATGGGCA AGTGTTCCAT 
GTCATTCCTT CCAGTCAGTC AGGAACAACT CAGTTTGTTA TTCCACAGGG GCAGGACATA 
GATGAGCAGC TGCAACAGAA TGAAAGCACT GAAAATCTGC AGGTAGTAGC AGGCAACACT 
GTGACAGAAA ATGCAGCCAG CTTTATTATT CATTCCAATG CATCAATGGC CATATCAAAG 
ATACCATCTG AGAGGTCACC TACAGAGTTG TTCCAAAAAC CTCTCCTACC ACTGCCAACA 
AACATTCCTG TTTGGGCTCG TCGTCTCAGG AACTGTGAGA AAATAGGAGA CTCTTACCGT 
GGTTACTGCT TAAGTGAGAA TGAGCTAATG AGCATTTTAG CACTACACAA ACAGCAAACA 
CAGAGTGTTT GGGGAACCCG CCAGTCTCCT AGTCCTGCTA AACCAGCTAC AAGACTTATG 
TGGAAGTCAC AGTATGTTCC ATATGACGGA ATTCCTTTTG TTAATGCAGG GAGCAGAGCA 
ATCGTTATGG AATGTCAGTA TGGGCCACGA AGAAAAGGAG CCCAACCCAA AAAGAGCGCT 
GAGAATGACA ACATGTCTGG GGGACTGTAC AAAGCAACTT GTCCTGCAAG GATATACATC 
AAAAAAGTAA AGAAGTTCCC TAATTACAGA GTGCCCACAG ATCCAAAGAT TGACAAGAAA 
CTTATAAGGA TGGAACAGGA GAAAGCCTTT AATTTGTTAA AGAAGGATCT GGATGGGACA 
GATGGAGTTC TCAGGTGGTA CATGCAGTTA CCAGATCAAG AAGCTCATCA GTATCATGAT 
CTGGAAACTG GTTGCTTTTC ACCATCAACT TTCCAGCCAT CCATTGAAGA TGAGGAGGAG 
ATTCTGTGTG ACGAGAACTC ACAACCCTCA CGGCTACATC CACAAGTAGC AGAAAAAATC 
CGTGATCTGG TGTCCAAAGG TTTGAGTGAG GTTTACTCAG TTAGGAAACA GCTTAGGAAA 
TTTGTGGAGC GGGATCTGTT TAAACCCGAT GACATACCTG AGCGCCATAA CTTATCCTAC 
TTTCCAACTG TGAATGATCT AAAAAATCAT ATTCATGAGG CACAGAAATC TTTGGCAAAC 
GAGGAGCTCA TTTATGATGC GGAAACAATC CCTGAGCCAG TTAGAGAGAT CCAGTGGACT 
GCTGACAGTG GAAACGTTCT TAATGAGATG GTTACTGTAA CATTTGCACC AATTCAACAG 
GAAGAACAAG AGACATTACT GAAAGTGGAG AATCCTCCCT CAGAGGATGT TTGCCAAGAC 
ACAGCTCAGC TGATCTCCTC CCTGACCTCA CTTCAGCCCA AAATCTTTGC TCAGCTACAG 
GGACTCCAGC TGCAACCGTC CTTAACATCA GTAGATGGAA AAACTGCTCT TATCACTGTG 
AATCCACCAG GTCCAACCTT TCTACCGGCT GAAGGTAAAA CTGCACTTAT TACTGTTAAT 
CCCGTGGACC AATCATTTAC AACTTCTGAT GGGAAAACTG CACTTTTTAC TGTAAATCCC 
CCTGCTCCTT CATTTACATC TGCTGATGGA ACTGCTGCAC TTATCACTGT GAATTCTCCT 
GCTCCTTCAT TTACATCAGC GGATGGGACA GCGGCATTGA TCACTGTGCA TCCCTCTGCC 
ATAGGCAGTT CTACATGCCT GTTGGGATCA GGAGGAAACA GTCAGCCTTT GGTGGTCAGT 
CATTTGCAGA ATGACTCCCC TGTGTCCTCT GTCACTAGTT TACCTGCCTC TGCCCACAAC 
CTAGTCAGTA TGAGCCAGAT TGTAACAGTG GAAGAGGCAA GTGGAATGGC TGGAGAGGTG 
CATCAAATTC TGCTGGGAGA TATCCACCCC ATTCCTATCA GGATAGTGGA GAACCAACAA 
ACAATTGTTG AAATAAATGC TCCTAATGCA TTTAGCACAG AGGATGTTGT AGCATTAAAT 
AAAGAGGTGA CTGAAGTACA GTTGAGTGCT ACAGAAGATA GTGCCACAGA TCCACAAACC 
CCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

carf ( XM_031892861.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748721.1 Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa66243
Clone ID Related Accession (Same CDS sequence)	XM_031892862.1 , XM_031892861.1 , XM_031892860.1
Accession Version	XM_031892861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calcium-responsive transcription factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTA ATGGACTGGA CGTTGATGTA CTGACTCGGC CTGCAGACTT GTTGATCACT 
TGTACCAATA CAGAGGATAA TGCTGATCTG GATGATGGGG TTCTCAAGAA TACTGCAAAC 
CAAATATCGA CCCTCACTGA GCAGTACCAT TTTATAGATC AAAGTGGAAA TACCATCCAC 
TATGCAATTC AGGCAGTGCC TGGTGCCAAC CCCCAAATGG ATTTTATAGT AACTTCCTTT 
TGCCATTTGT TGTTGAAGAT GATCCTTACC AGTGAATCAG AAAATGGGCA AGTGTTCCAT 
GTCATTCCTT CCAGTCAGTC AGGAACAACT CAGTTTGTTA TTCCACAGGG GCAGGACATA 
GATGAGCAGC TGCAACAGAA TGAAAGCACT GAAAATCTGC AGGTAGTAGC AGGCAACACT 
GTGACAGAAA ATGCAGCCAG CTTTATTATT CATTCCAATG CATCAATGGC CATATCAAAG 
ATACCATCTG AGAGGTCACC TACAGAGTTG TTCCAAAAAC CTCTCCTACC ACTGCCAACA 
AACATTCCTG TTTGGGCTCG TCGTCTCAGG AACTGTGAGA AAATAGGAGA CTCTTACCGT 
GGTTACTGCT TAAGTGAGAA TGAGCTAATG AGCATTTTAG CACTACACAA ACAGCAAACA 
CAGAGTGTTT GGGGAACCCG CCAGTCTCCT AGTCCTGCTA AACCAGCTAC AAGACTTATG 
TGGAAGTCAC AGTATGTTCC ATATGACGGA ATTCCTTTTG TTAATGCAGG GAGCAGAGCA 
ATCGTTATGG AATGTCAGTA TGGGCCACGA AGAAAAGGAG CCCAACCCAA AAAGAGCGCT 
GAGAATGACA ACATGTCTGG GGGACTGTAC AAAGCAACTT GTCCTGCAAG GATATACATC 
AAAAAAGTAA AGAAGTTCCC TAATTACAGA GTGCCCACAG ATCCAAAGAT TGACAAGAAA 
CTTATAAGGA TGGAACAGGA GAAAGCCTTT AATTTGTTAA AGAAGGATCT GGATGGGACA 
GATGGAGTTC TCAGGTGGTA CATGCAGTTA CCAGATCAAG AAGCTCATCA GTATCATGAT 
CTGGAAACTG GTTGCTTTTC ACCATCAACT TTCCAGCCAT CCATTGAAGA TGAGGAGGAG 
ATTCTGTGTG ACGAGAACTC ACAACCCTCA CGGCTACATC CACAAGTAGC AGAAAAAATC 
CGTGATCTGG TGTCCAAAGG TTTGAGTGAG GTTTACTCAG TTAGGAAACA GCTTAGGAAA 
TTTGTGGAGC GGGATCTGTT TAAACCCGAT GACATACCTG AGCGCCATAA CTTATCCTAC 
TTTCCAACTG TGAATGATCT AAAAAATCAT ATTCATGAGG CACAGAAATC TTTGGCAAAC 
GAGGAGCTCA TTTATGATGC GGAAACAATC CCTGAGCCAG TTAGAGAGAT CCAGTGGACT 
GCTGACAGTG GAAACGTTCT TAATGAGATG GTTACTGTAA CATTTGCACC AATTCAACAG 
GAAGAACAAG AGACATTACT GAAAGTGGAG AATCCTCCCT CAGAGGATGT TTGCCAAGAC 
ACAGCTCAGC TGATCTCCTC CCTGACCTCA CTTCAGCCCA AAATCTTTGC TCAGCTACAG 
GGACTCCAGC TGCAACCGTC CTTAACATCA GTAGATGGAA AAACTGCTCT TATCACTGTG 
AATCCACCAG GTCCAACCTT TCTACCGGCT GAAGGTAAAA CTGCACTTAT TACTGTTAAT 
CCCGTGGACC AATCATTTAC AACTTCTGAT GGGAAAACTG CACTTTTTAC TGTAAATCCC 
CCTGCTCCTT CATTTACATC TGCTGATGGA ACTGCTGCAC TTATCACTGT GAATTCTCCT 
GCTCCTTCAT TTACATCAGC GGATGGGACA GCGGCATTGA TCACTGTGCA TCCCTCTGCC 
ATAGGCAGTT CTACATGCCT GTTGGGATCA GGAGGAAACA GTCAGCCTTT GGTGGTCAGT 
CATTTGCAGA ATGACTCCCC TGTGTCCTCT GTCACTAGTT TACCTGCCTC TGCCCACAAC 
CTAGTCAGTA TGAGCCAGAT TGTAACAGTG GAAGAGGCAA GTGGAATGGC TGGAGAGGTG 
CATCAAATTC TGCTGGGAGA TATCCACCCC ATTCCTATCA GGATAGTGGA GAACCAACAA 
ACAATTGTTG AAATAAATGC TCCTAATGCA TTTAGCACAG AGGATGTTGT AGCATTAAAT 
AAAGAGGTGA CTGAAGTACA GTTGAGTGCT ACAGAAGATA GTGCCACAGA TCCACAAACC 
CCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748721.1
CDS	93..2381
Translation	MESNGLDVDVLTRPADLLITCTNTEDNADLDDGVLKNTANQISTLTEQYHFIDQSGNTIHYAIQAVPGANPQMDFIVTSFCHLLLKMILTSESENGQVFHVIPSSQSGTTQFVIPQGQDIDEQLQQNESTENLQVVAGNTVTENAASFIIHSNASMAISKIPSERSPTELFQKPLLPLPTNIPVWARRLRNCEKIGDSYRGYCLSENELMSILALHKQQTQSVWGTRQSPSPAKPATRLMWKSQYVPYDGIPFVNAGSRAIVMECQYGPRRKGAQPKKSAENDNMSGGLYKATCPARIYIKKVKKFPNYRVPTDPKIDKKLIRMEQEKAFNLLKKDLDGTDGVLRWYMQLPDQEAHQYHDLETGCFSPSTFQPSIEDEEEILCDENSQPSRLHPQVAEKIRDLVSKGLSEVYSVRKQLRKFVERDLFKPDDIPERHNLSYFPTVNDLKNHIHEAQKSLANEELIYDAETIPEPVREIQWTADSGNVLNEMVTVTFAPIQQEEQETLLKVENPPSEDVCQDTAQLISSLTSLQPKIFAQLQGLQLQPSLTSVDGKTALITVNPPGPTFLPAEGKTALITVNPVDQSFTTSDGKTALFTVNPPAPSFTSADGTAALITVNSPAPSFTSADGTAALITVHPSAIGSSTCLLGSGGNSQPLVVSHLQNDSPVSSVTSLPASAHNLVSMSQIVTVEEASGMAGEVHQILLGDIHPIPIRIVENQQTIVEINAPNAFSTEDVVALNKEVTEVQLSATEDSATDPQTPV

Target ORF information:

RefSeq Version	XM_031892861.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892861.1

ORF Insert Sequence:

ATGGAAAGTA ATGGACTGGA CGTTGATGTA CTGACTCGGC CTGCAGACTT GTTGATCACT 
TGTACCAATA CAGAGGATAA TGCTGATCTG GATGATGGGG TTCTCAAGAA TACTGCAAAC 
CAAATATCGA CCCTCACTGA GCAGTACCAT TTTATAGATC AAAGTGGAAA TACCATCCAC 
TATGCAATTC AGGCAGTGCC TGGTGCCAAC CCCCAAATGG ATTTTATAGT AACTTCCTTT 
TGCCATTTGT TGTTGAAGAT GATCCTTACC AGTGAATCAG AAAATGGGCA AGTGTTCCAT 
GTCATTCCTT CCAGTCAGTC AGGAACAACT CAGTTTGTTA TTCCACAGGG GCAGGACATA 
GATGAGCAGC TGCAACAGAA TGAAAGCACT GAAAATCTGC AGGTAGTAGC AGGCAACACT 
GTGACAGAAA ATGCAGCCAG CTTTATTATT CATTCCAATG CATCAATGGC CATATCAAAG 
ATACCATCTG AGAGGTCACC TACAGAGTTG TTCCAAAAAC CTCTCCTACC ACTGCCAACA 
AACATTCCTG TTTGGGCTCG TCGTCTCAGG AACTGTGAGA AAATAGGAGA CTCTTACCGT 
GGTTACTGCT TAAGTGAGAA TGAGCTAATG AGCATTTTAG CACTACACAA ACAGCAAACA 
CAGAGTGTTT GGGGAACCCG CCAGTCTCCT AGTCCTGCTA AACCAGCTAC AAGACTTATG 
TGGAAGTCAC AGTATGTTCC ATATGACGGA ATTCCTTTTG TTAATGCAGG GAGCAGAGCA 
ATCGTTATGG AATGTCAGTA TGGGCCACGA AGAAAAGGAG CCCAACCCAA AAAGAGCGCT 
GAGAATGACA ACATGTCTGG GGGACTGTAC AAAGCAACTT GTCCTGCAAG GATATACATC 
AAAAAAGTAA AGAAGTTCCC TAATTACAGA GTGCCCACAG ATCCAAAGAT TGACAAGAAA 
CTTATAAGGA TGGAACAGGA GAAAGCCTTT AATTTGTTAA AGAAGGATCT GGATGGGACA 
GATGGAGTTC TCAGGTGGTA CATGCAGTTA CCAGATCAAG AAGCTCATCA GTATCATGAT 
CTGGAAACTG GTTGCTTTTC ACCATCAACT TTCCAGCCAT CCATTGAAGA TGAGGAGGAG 
ATTCTGTGTG ACGAGAACTC ACAACCCTCA CGGCTACATC CACAAGTAGC AGAAAAAATC 
CGTGATCTGG TGTCCAAAGG TTTGAGTGAG GTTTACTCAG TTAGGAAACA GCTTAGGAAA 
TTTGTGGAGC GGGATCTGTT TAAACCCGAT GACATACCTG AGCGCCATAA CTTATCCTAC 
TTTCCAACTG TGAATGATCT AAAAAATCAT ATTCATGAGG CACAGAAATC TTTGGCAAAC 
GAGGAGCTCA TTTATGATGC GGAAACAATC CCTGAGCCAG TTAGAGAGAT CCAGTGGACT 
GCTGACAGTG GAAACGTTCT TAATGAGATG GTTACTGTAA CATTTGCACC AATTCAACAG 
GAAGAACAAG AGACATTACT GAAAGTGGAG AATCCTCCCT CAGAGGATGT TTGCCAAGAC 
ACAGCTCAGC TGATCTCCTC CCTGACCTCA CTTCAGCCCA AAATCTTTGC TCAGCTACAG 
GGACTCCAGC TGCAACCGTC CTTAACATCA GTAGATGGAA AAACTGCTCT TATCACTGTG 
AATCCACCAG GTCCAACCTT TCTACCGGCT GAAGGTAAAA CTGCACTTAT TACTGTTAAT 
CCCGTGGACC AATCATTTAC AACTTCTGAT GGGAAAACTG CACTTTTTAC TGTAAATCCC 
CCTGCTCCTT CATTTACATC TGCTGATGGA ACTGCTGCAC TTATCACTGT GAATTCTCCT 
GCTCCTTCAT TTACATCAGC GGATGGGACA GCGGCATTGA TCACTGTGCA TCCCTCTGCC 
ATAGGCAGTT CTACATGCCT GTTGGGATCA GGAGGAAACA GTCAGCCTTT GGTGGTCAGT 
CATTTGCAGA ATGACTCCCC TGTGTCCTCT GTCACTAGTT TACCTGCCTC TGCCCACAAC 
CTAGTCAGTA TGAGCCAGAT TGTAACAGTG GAAGAGGCAA GTGGAATGGC TGGAGAGGTG 
CATCAAATTC TGCTGGGAGA TATCCACCCC ATTCCTATCA GGATAGTGGA GAACCAACAA 
ACAATTGTTG AAATAAATGC TCCTAATGCA TTTAGCACAG AGGATGTTGT AGCATTAAAT 
AAAGAGGTGA CTGAAGTACA GTTGAGTGCT ACAGAAGATA GTGCCACAGA TCCACAAACC 
CCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

carf ( XM_031892860.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748720.1 Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa66243
Clone ID Related Accession (Same CDS sequence)	XM_031892862.1 , XM_031892861.1 , XM_031892860.1
Accession Version	XM_031892860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calcium-responsive transcription factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTA ATGGACTGGA CGTTGATGTA CTGACTCGGC CTGCAGACTT GTTGATCACT 
TGTACCAATA CAGAGGATAA TGCTGATCTG GATGATGGGG TTCTCAAGAA TACTGCAAAC 
CAAATATCGA CCCTCACTGA GCAGTACCAT TTTATAGATC AAAGTGGAAA TACCATCCAC 
TATGCAATTC AGGCAGTGCC TGGTGCCAAC CCCCAAATGG ATTTTATAGT AACTTCCTTT 
TGCCATTTGT TGTTGAAGAT GATCCTTACC AGTGAATCAG AAAATGGGCA AGTGTTCCAT 
GTCATTCCTT CCAGTCAGTC AGGAACAACT CAGTTTGTTA TTCCACAGGG GCAGGACATA 
GATGAGCAGC TGCAACAGAA TGAAAGCACT GAAAATCTGC AGGTAGTAGC AGGCAACACT 
GTGACAGAAA ATGCAGCCAG CTTTATTATT CATTCCAATG CATCAATGGC CATATCAAAG 
ATACCATCTG AGAGGTCACC TACAGAGTTG TTCCAAAAAC CTCTCCTACC ACTGCCAACA 
AACATTCCTG TTTGGGCTCG TCGTCTCAGG AACTGTGAGA AAATAGGAGA CTCTTACCGT 
GGTTACTGCT TAAGTGAGAA TGAGCTAATG AGCATTTTAG CACTACACAA ACAGCAAACA 
CAGAGTGTTT GGGGAACCCG CCAGTCTCCT AGTCCTGCTA AACCAGCTAC AAGACTTATG 
TGGAAGTCAC AGTATGTTCC ATATGACGGA ATTCCTTTTG TTAATGCAGG GAGCAGAGCA 
ATCGTTATGG AATGTCAGTA TGGGCCACGA AGAAAAGGAG CCCAACCCAA AAAGAGCGCT 
GAGAATGACA ACATGTCTGG GGGACTGTAC AAAGCAACTT GTCCTGCAAG GATATACATC 
AAAAAAGTAA AGAAGTTCCC TAATTACAGA GTGCCCACAG ATCCAAAGAT TGACAAGAAA 
CTTATAAGGA TGGAACAGGA GAAAGCCTTT AATTTGTTAA AGAAGGATCT GGATGGGACA 
GATGGAGTTC TCAGGTGGTA CATGCAGTTA CCAGATCAAG AAGCTCATCA GTATCATGAT 
CTGGAAACTG GTTGCTTTTC ACCATCAACT TTCCAGCCAT CCATTGAAGA TGAGGAGGAG 
ATTCTGTGTG ACGAGAACTC ACAACCCTCA CGGCTACATC CACAAGTAGC AGAAAAAATC 
CGTGATCTGG TGTCCAAAGG TTTGAGTGAG GTTTACTCAG TTAGGAAACA GCTTAGGAAA 
TTTGTGGAGC GGGATCTGTT TAAACCCGAT GACATACCTG AGCGCCATAA CTTATCCTAC 
TTTCCAACTG TGAATGATCT AAAAAATCAT ATTCATGAGG CACAGAAATC TTTGGCAAAC 
GAGGAGCTCA TTTATGATGC GGAAACAATC CCTGAGCCAG TTAGAGAGAT CCAGTGGACT 
GCTGACAGTG GAAACGTTCT TAATGAGATG GTTACTGTAA CATTTGCACC AATTCAACAG 
GAAGAACAAG AGACATTACT GAAAGTGGAG AATCCTCCCT CAGAGGATGT TTGCCAAGAC 
ACAGCTCAGC TGATCTCCTC CCTGACCTCA CTTCAGCCCA AAATCTTTGC TCAGCTACAG 
GGACTCCAGC TGCAACCGTC CTTAACATCA GTAGATGGAA AAACTGCTCT TATCACTGTG 
AATCCACCAG GTCCAACCTT TCTACCGGCT GAAGGTAAAA CTGCACTTAT TACTGTTAAT 
CCCGTGGACC AATCATTTAC AACTTCTGAT GGGAAAACTG CACTTTTTAC TGTAAATCCC 
CCTGCTCCTT CATTTACATC TGCTGATGGA ACTGCTGCAC TTATCACTGT GAATTCTCCT 
GCTCCTTCAT TTACATCAGC GGATGGGACA GCGGCATTGA TCACTGTGCA TCCCTCTGCC 
ATAGGCAGTT CTACATGCCT GTTGGGATCA GGAGGAAACA GTCAGCCTTT GGTGGTCAGT 
CATTTGCAGA ATGACTCCCC TGTGTCCTCT GTCACTAGTT TACCTGCCTC TGCCCACAAC 
CTAGTCAGTA TGAGCCAGAT TGTAACAGTG GAAGAGGCAA GTGGAATGGC TGGAGAGGTG 
CATCAAATTC TGCTGGGAGA TATCCACCCC ATTCCTATCA GGATAGTGGA GAACCAACAA 
ACAATTGTTG AAATAAATGC TCCTAATGCA TTTAGCACAG AGGATGTTGT AGCATTAAAT 
AAAGAGGTGA CTGAAGTACA GTTGAGTGCT ACAGAAGATA GTGCCACAGA TCCACAAACC 
CCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748720.1
CDS	98..2386
Translation	MESNGLDVDVLTRPADLLITCTNTEDNADLDDGVLKNTANQISTLTEQYHFIDQSGNTIHYAIQAVPGANPQMDFIVTSFCHLLLKMILTSESENGQVFHVIPSSQSGTTQFVIPQGQDIDEQLQQNESTENLQVVAGNTVTENAASFIIHSNASMAISKIPSERSPTELFQKPLLPLPTNIPVWARRLRNCEKIGDSYRGYCLSENELMSILALHKQQTQSVWGTRQSPSPAKPATRLMWKSQYVPYDGIPFVNAGSRAIVMECQYGPRRKGAQPKKSAENDNMSGGLYKATCPARIYIKKVKKFPNYRVPTDPKIDKKLIRMEQEKAFNLLKKDLDGTDGVLRWYMQLPDQEAHQYHDLETGCFSPSTFQPSIEDEEEILCDENSQPSRLHPQVAEKIRDLVSKGLSEVYSVRKQLRKFVERDLFKPDDIPERHNLSYFPTVNDLKNHIHEAQKSLANEELIYDAETIPEPVREIQWTADSGNVLNEMVTVTFAPIQQEEQETLLKVENPPSEDVCQDTAQLISSLTSLQPKIFAQLQGLQLQPSLTSVDGKTALITVNPPGPTFLPAEGKTALITVNPVDQSFTTSDGKTALFTVNPPAPSFTSADGTAALITVNSPAPSFTSADGTAALITVHPSAIGSSTCLLGSGGNSQPLVVSHLQNDSPVSSVTSLPASAHNLVSMSQIVTVEEASGMAGEVHQILLGDIHPIPIRIVENQQTIVEINAPNAFSTEDVVALNKEVTEVQLSATEDSATDPQTPV

Target ORF information:

RefSeq Version	XM_031892860.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892860.1

ORF Insert Sequence:

ATGGAAAGTA ATGGACTGGA CGTTGATGTA CTGACTCGGC CTGCAGACTT GTTGATCACT 
TGTACCAATA CAGAGGATAA TGCTGATCTG GATGATGGGG TTCTCAAGAA TACTGCAAAC 
CAAATATCGA CCCTCACTGA GCAGTACCAT TTTATAGATC AAAGTGGAAA TACCATCCAC 
TATGCAATTC AGGCAGTGCC TGGTGCCAAC CCCCAAATGG ATTTTATAGT AACTTCCTTT 
TGCCATTTGT TGTTGAAGAT GATCCTTACC AGTGAATCAG AAAATGGGCA AGTGTTCCAT 
GTCATTCCTT CCAGTCAGTC AGGAACAACT CAGTTTGTTA TTCCACAGGG GCAGGACATA 
GATGAGCAGC TGCAACAGAA TGAAAGCACT GAAAATCTGC AGGTAGTAGC AGGCAACACT 
GTGACAGAAA ATGCAGCCAG CTTTATTATT CATTCCAATG CATCAATGGC CATATCAAAG 
ATACCATCTG AGAGGTCACC TACAGAGTTG TTCCAAAAAC CTCTCCTACC ACTGCCAACA 
AACATTCCTG TTTGGGCTCG TCGTCTCAGG AACTGTGAGA AAATAGGAGA CTCTTACCGT 
GGTTACTGCT TAAGTGAGAA TGAGCTAATG AGCATTTTAG CACTACACAA ACAGCAAACA 
CAGAGTGTTT GGGGAACCCG CCAGTCTCCT AGTCCTGCTA AACCAGCTAC AAGACTTATG 
TGGAAGTCAC AGTATGTTCC ATATGACGGA ATTCCTTTTG TTAATGCAGG GAGCAGAGCA 
ATCGTTATGG AATGTCAGTA TGGGCCACGA AGAAAAGGAG CCCAACCCAA AAAGAGCGCT 
GAGAATGACA ACATGTCTGG GGGACTGTAC AAAGCAACTT GTCCTGCAAG GATATACATC 
AAAAAAGTAA AGAAGTTCCC TAATTACAGA GTGCCCACAG ATCCAAAGAT TGACAAGAAA 
CTTATAAGGA TGGAACAGGA GAAAGCCTTT AATTTGTTAA AGAAGGATCT GGATGGGACA 
GATGGAGTTC TCAGGTGGTA CATGCAGTTA CCAGATCAAG AAGCTCATCA GTATCATGAT 
CTGGAAACTG GTTGCTTTTC ACCATCAACT TTCCAGCCAT CCATTGAAGA TGAGGAGGAG 
ATTCTGTGTG ACGAGAACTC ACAACCCTCA CGGCTACATC CACAAGTAGC AGAAAAAATC 
CGTGATCTGG TGTCCAAAGG TTTGAGTGAG GTTTACTCAG TTAGGAAACA GCTTAGGAAA 
TTTGTGGAGC GGGATCTGTT TAAACCCGAT GACATACCTG AGCGCCATAA CTTATCCTAC 
TTTCCAACTG TGAATGATCT AAAAAATCAT ATTCATGAGG CACAGAAATC TTTGGCAAAC 
GAGGAGCTCA TTTATGATGC GGAAACAATC CCTGAGCCAG TTAGAGAGAT CCAGTGGACT 
GCTGACAGTG GAAACGTTCT TAATGAGATG GTTACTGTAA CATTTGCACC AATTCAACAG 
GAAGAACAAG AGACATTACT GAAAGTGGAG AATCCTCCCT CAGAGGATGT TTGCCAAGAC 
ACAGCTCAGC TGATCTCCTC CCTGACCTCA CTTCAGCCCA AAATCTTTGC TCAGCTACAG 
GGACTCCAGC TGCAACCGTC CTTAACATCA GTAGATGGAA AAACTGCTCT TATCACTGTG 
AATCCACCAG GTCCAACCTT TCTACCGGCT GAAGGTAAAA CTGCACTTAT TACTGTTAAT 
CCCGTGGACC AATCATTTAC AACTTCTGAT GGGAAAACTG CACTTTTTAC TGTAAATCCC 
CCTGCTCCTT CATTTACATC TGCTGATGGA ACTGCTGCAC TTATCACTGT GAATTCTCCT 
GCTCCTTCAT TTACATCAGC GGATGGGACA GCGGCATTGA TCACTGTGCA TCCCTCTGCC 
ATAGGCAGTT CTACATGCCT GTTGGGATCA GGAGGAAACA GTCAGCCTTT GGTGGTCAGT 
CATTTGCAGA ATGACTCCCC TGTGTCCTCT GTCACTAGTT TACCTGCCTC TGCCCACAAC 
CTAGTCAGTA TGAGCCAGAT TGTAACAGTG GAAGAGGCAA GTGGAATGGC TGGAGAGGTG 
CATCAAATTC TGCTGGGAGA TATCCACCCC ATTCCTATCA GGATAGTGGA GAACCAACAA 
ACAATTGTTG AAATAAATGC TCCTAATGCA TTTAGCACAG AGGATGTTGT AGCATTAAAT 
AAAGAGGTGA CTGAAGTACA GTTGAGTGCT ACAGAAGATA GTGCCACAGA TCCACAAACC 
CCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

carf ( XM_012971179.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826633.3 Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa66244
Clone ID Related Accession (Same CDS sequence)	XM_012971179.3 , XM_031892863.1
Accession Version	XM_012971179.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calcium-responsive transcription factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012971179.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTA ATGGACTGGA CGTTGATGTA CTGACTCGGC CTGCAGACTT GTTGATCACT 
TGTACCAATA CAGAGGATAA TGCTGATCTG GATGATGGGG TTCTCAAGAA TACTGCAAAC 
CAAATATCGA CCCTCACTGA GCAGTACCAT TTTATAGATC AAAGTGGAAA TACCATCCAC 
TATGCAATTC AGGCAGTGCC TGGTGCCAAC CCCCAAATGA TGATCCTTAC CAGTGAATCA 
GAAAATGGGC AAGTGTTCCA TGTCATTCCT TCCAGTCAGT CAGGAACAAC TCAGTTTGTT 
ATTCCACAGG GGCAGGACAT AGATGAGCAG CTGCAACAGA ATGAAAGCAC TGAAAATCTG 
CAGGTAGTAG CAGGCAACAC TGTGACAGAA AATGCAGCCA GCTTTATTAT TCATTCCAAT 
GCATCAATGG CCATATCAAA GATACCATCT GAGAGGTCAC CTACAGAGTT GTTCCAAAAA 
CCTCTCCTAC CACTGCCAAC AAACATTCCT GTTTGGGCTC GTCGTCTCAG GAACTGTGAG 
AAAATAGGAG ACTCTTACCG TGGTTACTGC TTAAGTGAGA ATGAGCTAAT GAGCATTTTA 
GCACTACACA AACAGCAAAC ACAGAGTGTT TGGGGAACCC GCCAGTCTCC TAGTCCTGCT 
AAACCAGCTA CAAGACTTAT GTGGAAGTCA CAGTATGTTC CATATGACGG AATTCCTTTT 
GTTAATGCAG GGAGCAGAGC AATCGTTATG GAATGTCAGT ATGGGCCACG AAGAAAAGGA 
GCCCAACCCA AAAAGAGCGC TGAGAATGAC AACATGTCTG GGGGACTGTA CAAAGCAACT 
TGTCCTGCAA GGATATACAT CAAAAAAGTA AAGAAGTTCC CTAATTACAG AGTGCCCACA 
GATCCAAAGA TTGACAAGAA ACTTATAAGG ATGGAACAGG AGAAAGCCTT TAATTTGTTA 
AAGAAGGATC TGGATGGGAC AGATGGAGTT CTCAGGTGGT ACATGCAGTT ACCAGATCAA 
GAAGCTCATC AGTATCATGA TCTGGAAACT GGTTGCTTTT CACCATCAAC TTTCCAGCCA 
TCCATTGAAG ATGAGGAGGA GATTCTGTGT GACGAGAACT CACAACCCTC ACGGCTACAT 
CCACAAGTAG CAGAAAAAAT CCGTGATCTG GTGTCCAAAG GTTTGAGTGA GGTTTACTCA 
GTTAGGAAAC AGCTTAGGAA ATTTGTGGAG CGGGATCTGT TTAAACCCGA TGACATACCT 
GAGCGCCATA ACTTATCCTA CTTTCCAACT GTGAATGATC TAAAAAATCA TATTCATGAG 
GCACAGAAAT CTTTGGCAAA CGAGGAGCTC ATTTATGATG CGGAAACAAT CCCTGAGCCA 
GTTAGAGAGA TCCAGTGGAC TGCTGACAGT GGAAACGTTC TTAATGAGAT GGTTACTGTA 
ACATTTGCAC CAATTCAACA GGAAGAACAA GAGACATTAC TGAAAGTGGA GAATCCTCCC 
TCAGAGGATG TTTGCCAAGA CACAGCTCAG CTGATCTCCT CCCTGACCTC ACTTCAGCCC 
AAAATCTTTG CTCAGCTACA GGGACTCCAG CTGCAACCGT CCTTAACATC AGTAGATGGA 
AAAACTGCTC TTATCACTGT GAATCCACCA GGTCCAACCT TTCTACCGGC TGAAGGTAAA 
ACTGCACTTA TTACTGTTAA TCCCGTGGAC CAATCATTTA CAACTTCTGA TGGGAAAACT 
GCACTTTTTA CTGTAAATCC CCCTGCTCCT TCATTTACAT CTGCTGATGG AACTGCTGCA 
CTTATCACTG TGAATTCTCC TGCTCCTTCA TTTACATCAG CGGATGGGAC AGCGGCATTG 
ATCACTGTGC ATCCCTCTGC CATAGGCAGT TCTACATGCC TGTTGGGATC AGGAGGAAAC 
AGTCAGCCTT TGGTGGTCAG TCATTTGCAG AATGACTCCC CTGTGTCCTC TGTCACTAGT 
TTACCTGCCT CTGCCCACAA CCTAGTCAGT ATGAGCCAGA TTGTAACAGT GGAAGAGGCA 
AGTGGAATGG CTGGAGAGGT GCATCAAATT CTGCTGGGAG ATATCCACCC CATTCCTATC 
AGGATAGTGG AGAACCAACA AACAATTGTT GAAATAAATG CTCCTAATGC ATTTAGCACA 
GAGGATGTTG TAGCATTAAA TAAAGAGGTG ACTGAAGTAC AGTTGAGTGC TACAGAAGAT 
AGTGCCACAG ATCCACAAAC CCCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826633.3
CDS	98..2347
Translation	MESNGLDVDVLTRPADLLITCTNTEDNADLDDGVLKNTANQISTLTEQYHFIDQSGNTIHYAIQAVPGANPQMMILTSESENGQVFHVIPSSQSGTTQFVIPQGQDIDEQLQQNESTENLQVVAGNTVTENAASFIIHSNASMAISKIPSERSPTELFQKPLLPLPTNIPVWARRLRNCEKIGDSYRGYCLSENELMSILALHKQQTQSVWGTRQSPSPAKPATRLMWKSQYVPYDGIPFVNAGSRAIVMECQYGPRRKGAQPKKSAENDNMSGGLYKATCPARIYIKKVKKFPNYRVPTDPKIDKKLIRMEQEKAFNLLKKDLDGTDGVLRWYMQLPDQEAHQYHDLETGCFSPSTFQPSIEDEEEILCDENSQPSRLHPQVAEKIRDLVSKGLSEVYSVRKQLRKFVERDLFKPDDIPERHNLSYFPTVNDLKNHIHEAQKSLANEELIYDAETIPEPVREIQWTADSGNVLNEMVTVTFAPIQQEEQETLLKVENPPSEDVCQDTAQLISSLTSLQPKIFAQLQGLQLQPSLTSVDGKTALITVNPPGPTFLPAEGKTALITVNPVDQSFTTSDGKTALFTVNPPAPSFTSADGTAALITVNSPAPSFTSADGTAALITVHPSAIGSSTCLLGSGGNSQPLVVSHLQNDSPVSSVTSLPASAHNLVSMSQIVTVEEASGMAGEVHQILLGDIHPIPIRIVENQQTIVEINAPNAFSTEDVVALNKEVTEVQLSATEDSATDPQTPV

Target ORF information:

RefSeq Version	XM_012971179.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971179.3

ORF Insert Sequence:

ATGGAAAGTA ATGGACTGGA CGTTGATGTA CTGACTCGGC CTGCAGACTT GTTGATCACT 
TGTACCAATA CAGAGGATAA TGCTGATCTG GATGATGGGG TTCTCAAGAA TACTGCAAAC 
CAAATATCGA CCCTCACTGA GCAGTACCAT TTTATAGATC AAAGTGGAAA TACCATCCAC 
TATGCAATTC AGGCAGTGCC TGGTGCCAAC CCCCAAATGA TGATCCTTAC CAGTGAATCA 
GAAAATGGGC AAGTGTTCCA TGTCATTCCT TCCAGTCAGT CAGGAACAAC TCAGTTTGTT 
ATTCCACAGG GGCAGGACAT AGATGAGCAG CTGCAACAGA ATGAAAGCAC TGAAAATCTG 
CAGGTAGTAG CAGGCAACAC TGTGACAGAA AATGCAGCCA GCTTTATTAT TCATTCCAAT 
GCATCAATGG CCATATCAAA GATACCATCT GAGAGGTCAC CTACAGAGTT GTTCCAAAAA 
CCTCTCCTAC CACTGCCAAC AAACATTCCT GTTTGGGCTC GTCGTCTCAG GAACTGTGAG 
AAAATAGGAG ACTCTTACCG TGGTTACTGC TTAAGTGAGA ATGAGCTAAT GAGCATTTTA 
GCACTACACA AACAGCAAAC ACAGAGTGTT TGGGGAACCC GCCAGTCTCC TAGTCCTGCT 
AAACCAGCTA CAAGACTTAT GTGGAAGTCA CAGTATGTTC CATATGACGG AATTCCTTTT 
GTTAATGCAG GGAGCAGAGC AATCGTTATG GAATGTCAGT ATGGGCCACG AAGAAAAGGA 
GCCCAACCCA AAAAGAGCGC TGAGAATGAC AACATGTCTG GGGGACTGTA CAAAGCAACT 
TGTCCTGCAA GGATATACAT CAAAAAAGTA AAGAAGTTCC CTAATTACAG AGTGCCCACA 
GATCCAAAGA TTGACAAGAA ACTTATAAGG ATGGAACAGG AGAAAGCCTT TAATTTGTTA 
AAGAAGGATC TGGATGGGAC AGATGGAGTT CTCAGGTGGT ACATGCAGTT ACCAGATCAA 
GAAGCTCATC AGTATCATGA TCTGGAAACT GGTTGCTTTT CACCATCAAC TTTCCAGCCA 
TCCATTGAAG ATGAGGAGGA GATTCTGTGT GACGAGAACT CACAACCCTC ACGGCTACAT 
CCACAAGTAG CAGAAAAAAT CCGTGATCTG GTGTCCAAAG GTTTGAGTGA GGTTTACTCA 
GTTAGGAAAC AGCTTAGGAA ATTTGTGGAG CGGGATCTGT TTAAACCCGA TGACATACCT 
GAGCGCCATA ACTTATCCTA CTTTCCAACT GTGAATGATC TAAAAAATCA TATTCATGAG 
GCACAGAAAT CTTTGGCAAA CGAGGAGCTC ATTTATGATG CGGAAACAAT CCCTGAGCCA 
GTTAGAGAGA TCCAGTGGAC TGCTGACAGT GGAAACGTTC TTAATGAGAT GGTTACTGTA 
ACATTTGCAC CAATTCAACA GGAAGAACAA GAGACATTAC TGAAAGTGGA GAATCCTCCC 
TCAGAGGATG TTTGCCAAGA CACAGCTCAG CTGATCTCCT CCCTGACCTC ACTTCAGCCC 
AAAATCTTTG CTCAGCTACA GGGACTCCAG CTGCAACCGT CCTTAACATC AGTAGATGGA 
AAAACTGCTC TTATCACTGT GAATCCACCA GGTCCAACCT TTCTACCGGC TGAAGGTAAA 
ACTGCACTTA TTACTGTTAA TCCCGTGGAC CAATCATTTA CAACTTCTGA TGGGAAAACT 
GCACTTTTTA CTGTAAATCC CCCTGCTCCT TCATTTACAT CTGCTGATGG AACTGCTGCA 
CTTATCACTG TGAATTCTCC TGCTCCTTCA TTTACATCAG CGGATGGGAC AGCGGCATTG 
ATCACTGTGC ATCCCTCTGC CATAGGCAGT TCTACATGCC TGTTGGGATC AGGAGGAAAC 
AGTCAGCCTT TGGTGGTCAG TCATTTGCAG AATGACTCCC CTGTGTCCTC TGTCACTAGT 
TTACCTGCCT CTGCCCACAA CCTAGTCAGT ATGAGCCAGA TTGTAACAGT GGAAGAGGCA 
AGTGGAATGG CTGGAGAGGT GCATCAAATT CTGCTGGGAG ATATCCACCC CATTCCTATC 
AGGATAGTGG AGAACCAACA AACAATTGTT GAAATAAATG CTCCTAATGC ATTTAGCACA 
GAGGATGTTG TAGCATTAAA TAAAGAGGTG ACTGAAGTAC AGTTGAGTGC TACAGAAGAT 
AGTGCCACAG ATCCACAAAC CCCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

carf ( XM_031892863.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748723.1 Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa66244
Clone ID Related Accession (Same CDS sequence)	XM_012971179.3 , XM_031892863.1
Accession Version	XM_031892863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	calcium-responsive transcription factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTA ATGGACTGGA CGTTGATGTA CTGACTCGGC CTGCAGACTT GTTGATCACT 
TGTACCAATA CAGAGGATAA TGCTGATCTG GATGATGGGG TTCTCAAGAA TACTGCAAAC 
CAAATATCGA CCCTCACTGA GCAGTACCAT TTTATAGATC AAAGTGGAAA TACCATCCAC 
TATGCAATTC AGGCAGTGCC TGGTGCCAAC CCCCAAATGA TGATCCTTAC CAGTGAATCA 
GAAAATGGGC AAGTGTTCCA TGTCATTCCT TCCAGTCAGT CAGGAACAAC TCAGTTTGTT 
ATTCCACAGG GGCAGGACAT AGATGAGCAG CTGCAACAGA ATGAAAGCAC TGAAAATCTG 
CAGGTAGTAG CAGGCAACAC TGTGACAGAA AATGCAGCCA GCTTTATTAT TCATTCCAAT 
GCATCAATGG CCATATCAAA GATACCATCT GAGAGGTCAC CTACAGAGTT GTTCCAAAAA 
CCTCTCCTAC CACTGCCAAC AAACATTCCT GTTTGGGCTC GTCGTCTCAG GAACTGTGAG 
AAAATAGGAG ACTCTTACCG TGGTTACTGC TTAAGTGAGA ATGAGCTAAT GAGCATTTTA 
GCACTACACA AACAGCAAAC ACAGAGTGTT TGGGGAACCC GCCAGTCTCC TAGTCCTGCT 
AAACCAGCTA CAAGACTTAT GTGGAAGTCA CAGTATGTTC CATATGACGG AATTCCTTTT 
GTTAATGCAG GGAGCAGAGC AATCGTTATG GAATGTCAGT ATGGGCCACG AAGAAAAGGA 
GCCCAACCCA AAAAGAGCGC TGAGAATGAC AACATGTCTG GGGGACTGTA CAAAGCAACT 
TGTCCTGCAA GGATATACAT CAAAAAAGTA AAGAAGTTCC CTAATTACAG AGTGCCCACA 
GATCCAAAGA TTGACAAGAA ACTTATAAGG ATGGAACAGG AGAAAGCCTT TAATTTGTTA 
AAGAAGGATC TGGATGGGAC AGATGGAGTT CTCAGGTGGT ACATGCAGTT ACCAGATCAA 
GAAGCTCATC AGTATCATGA TCTGGAAACT GGTTGCTTTT CACCATCAAC TTTCCAGCCA 
TCCATTGAAG ATGAGGAGGA GATTCTGTGT GACGAGAACT CACAACCCTC ACGGCTACAT 
CCACAAGTAG CAGAAAAAAT CCGTGATCTG GTGTCCAAAG GTTTGAGTGA GGTTTACTCA 
GTTAGGAAAC AGCTTAGGAA ATTTGTGGAG CGGGATCTGT TTAAACCCGA TGACATACCT 
GAGCGCCATA ACTTATCCTA CTTTCCAACT GTGAATGATC TAAAAAATCA TATTCATGAG 
GCACAGAAAT CTTTGGCAAA CGAGGAGCTC ATTTATGATG CGGAAACAAT CCCTGAGCCA 
GTTAGAGAGA TCCAGTGGAC TGCTGACAGT GGAAACGTTC TTAATGAGAT GGTTACTGTA 
ACATTTGCAC CAATTCAACA GGAAGAACAA GAGACATTAC TGAAAGTGGA GAATCCTCCC 
TCAGAGGATG TTTGCCAAGA CACAGCTCAG CTGATCTCCT CCCTGACCTC ACTTCAGCCC 
AAAATCTTTG CTCAGCTACA GGGACTCCAG CTGCAACCGT CCTTAACATC AGTAGATGGA 
AAAACTGCTC TTATCACTGT GAATCCACCA GGTCCAACCT TTCTACCGGC TGAAGGTAAA 
ACTGCACTTA TTACTGTTAA TCCCGTGGAC CAATCATTTA CAACTTCTGA TGGGAAAACT 
GCACTTTTTA CTGTAAATCC CCCTGCTCCT TCATTTACAT CTGCTGATGG AACTGCTGCA 
CTTATCACTG TGAATTCTCC TGCTCCTTCA TTTACATCAG CGGATGGGAC AGCGGCATTG 
ATCACTGTGC ATCCCTCTGC CATAGGCAGT TCTACATGCC TGTTGGGATC AGGAGGAAAC 
AGTCAGCCTT TGGTGGTCAG TCATTTGCAG AATGACTCCC CTGTGTCCTC TGTCACTAGT 
TTACCTGCCT CTGCCCACAA CCTAGTCAGT ATGAGCCAGA TTGTAACAGT GGAAGAGGCA 
AGTGGAATGG CTGGAGAGGT GCATCAAATT CTGCTGGGAG ATATCCACCC CATTCCTATC 
AGGATAGTGG AGAACCAACA AACAATTGTT GAAATAAATG CTCCTAATGC ATTTAGCACA 
GAGGATGTTG TAGCATTAAA TAAAGAGGTG ACTGAAGTAC AGTTGAGTGC TACAGAAGAT 
AGTGCCACAG ATCCACAAAC CCCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748723.1
CDS	668..2917
Translation	MESNGLDVDVLTRPADLLITCTNTEDNADLDDGVLKNTANQISTLTEQYHFIDQSGNTIHYAIQAVPGANPQMMILTSESENGQVFHVIPSSQSGTTQFVIPQGQDIDEQLQQNESTENLQVVAGNTVTENAASFIIHSNASMAISKIPSERSPTELFQKPLLPLPTNIPVWARRLRNCEKIGDSYRGYCLSENELMSILALHKQQTQSVWGTRQSPSPAKPATRLMWKSQYVPYDGIPFVNAGSRAIVMECQYGPRRKGAQPKKSAENDNMSGGLYKATCPARIYIKKVKKFPNYRVPTDPKIDKKLIRMEQEKAFNLLKKDLDGTDGVLRWYMQLPDQEAHQYHDLETGCFSPSTFQPSIEDEEEILCDENSQPSRLHPQVAEKIRDLVSKGLSEVYSVRKQLRKFVERDLFKPDDIPERHNLSYFPTVNDLKNHIHEAQKSLANEELIYDAETIPEPVREIQWTADSGNVLNEMVTVTFAPIQQEEQETLLKVENPPSEDVCQDTAQLISSLTSLQPKIFAQLQGLQLQPSLTSVDGKTALITVNPPGPTFLPAEGKTALITVNPVDQSFTTSDGKTALFTVNPPAPSFTSADGTAALITVNSPAPSFTSADGTAALITVHPSAIGSSTCLLGSGGNSQPLVVSHLQNDSPVSSVTSLPASAHNLVSMSQIVTVEEASGMAGEVHQILLGDIHPIPIRIVENQQTIVEINAPNAFSTEDVVALNKEVTEVQLSATEDSATDPQTPV

Target ORF information:

RefSeq Version	XM_031892863.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium responsive transcription factor (carf), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892863.1

ORF Insert Sequence:

ATGGAAAGTA ATGGACTGGA CGTTGATGTA CTGACTCGGC CTGCAGACTT GTTGATCACT 
TGTACCAATA CAGAGGATAA TGCTGATCTG GATGATGGGG TTCTCAAGAA TACTGCAAAC 
CAAATATCGA CCCTCACTGA GCAGTACCAT TTTATAGATC AAAGTGGAAA TACCATCCAC 
TATGCAATTC AGGCAGTGCC TGGTGCCAAC CCCCAAATGA TGATCCTTAC CAGTGAATCA 
GAAAATGGGC AAGTGTTCCA TGTCATTCCT TCCAGTCAGT CAGGAACAAC TCAGTTTGTT 
ATTCCACAGG GGCAGGACAT AGATGAGCAG CTGCAACAGA ATGAAAGCAC TGAAAATCTG 
CAGGTAGTAG CAGGCAACAC TGTGACAGAA AATGCAGCCA GCTTTATTAT TCATTCCAAT 
GCATCAATGG CCATATCAAA GATACCATCT GAGAGGTCAC CTACAGAGTT GTTCCAAAAA 
CCTCTCCTAC CACTGCCAAC AAACATTCCT GTTTGGGCTC GTCGTCTCAG GAACTGTGAG 
AAAATAGGAG ACTCTTACCG TGGTTACTGC TTAAGTGAGA ATGAGCTAAT GAGCATTTTA 
GCACTACACA AACAGCAAAC ACAGAGTGTT TGGGGAACCC GCCAGTCTCC TAGTCCTGCT 
AAACCAGCTA CAAGACTTAT GTGGAAGTCA CAGTATGTTC CATATGACGG AATTCCTTTT 
GTTAATGCAG GGAGCAGAGC AATCGTTATG GAATGTCAGT ATGGGCCACG AAGAAAAGGA 
GCCCAACCCA AAAAGAGCGC TGAGAATGAC AACATGTCTG GGGGACTGTA CAAAGCAACT 
TGTCCTGCAA GGATATACAT CAAAAAAGTA AAGAAGTTCC CTAATTACAG AGTGCCCACA 
GATCCAAAGA TTGACAAGAA ACTTATAAGG ATGGAACAGG AGAAAGCCTT TAATTTGTTA 
AAGAAGGATC TGGATGGGAC AGATGGAGTT CTCAGGTGGT ACATGCAGTT ACCAGATCAA 
GAAGCTCATC AGTATCATGA TCTGGAAACT GGTTGCTTTT CACCATCAAC TTTCCAGCCA 
TCCATTGAAG ATGAGGAGGA GATTCTGTGT GACGAGAACT CACAACCCTC ACGGCTACAT 
CCACAAGTAG CAGAAAAAAT CCGTGATCTG GTGTCCAAAG GTTTGAGTGA GGTTTACTCA 
GTTAGGAAAC AGCTTAGGAA ATTTGTGGAG CGGGATCTGT TTAAACCCGA TGACATACCT 
GAGCGCCATA ACTTATCCTA CTTTCCAACT GTGAATGATC TAAAAAATCA TATTCATGAG 
GCACAGAAAT CTTTGGCAAA CGAGGAGCTC ATTTATGATG CGGAAACAAT CCCTGAGCCA 
GTTAGAGAGA TCCAGTGGAC TGCTGACAGT GGAAACGTTC TTAATGAGAT GGTTACTGTA 
ACATTTGCAC CAATTCAACA GGAAGAACAA GAGACATTAC TGAAAGTGGA GAATCCTCCC 
TCAGAGGATG TTTGCCAAGA CACAGCTCAG CTGATCTCCT CCCTGACCTC ACTTCAGCCC 
AAAATCTTTG CTCAGCTACA GGGACTCCAG CTGCAACCGT CCTTAACATC AGTAGATGGA 
AAAACTGCTC TTATCACTGT GAATCCACCA GGTCCAACCT TTCTACCGGC TGAAGGTAAA 
ACTGCACTTA TTACTGTTAA TCCCGTGGAC CAATCATTTA CAACTTCTGA TGGGAAAACT 
GCACTTTTTA CTGTAAATCC CCCTGCTCCT TCATTTACAT CTGCTGATGG AACTGCTGCA 
CTTATCACTG TGAATTCTCC TGCTCCTTCA TTTACATCAG CGGATGGGAC AGCGGCATTG 
ATCACTGTGC ATCCCTCTGC CATAGGCAGT TCTACATGCC TGTTGGGATC AGGAGGAAAC 
AGTCAGCCTT TGGTGGTCAG TCATTTGCAG AATGACTCCC CTGTGTCCTC TGTCACTAGT 
TTACCTGCCT CTGCCCACAA CCTAGTCAGT ATGAGCCAGA TTGTAACAGT GGAAGAGGCA 
AGTGGAATGG CTGGAGAGGT GCATCAAATT CTGCTGGGAG ATATCCACCC CATTCCTATC 
AGGATAGTGG AGAACCAACA AACAATTGTT GAAATAAATG CTCCTAATGC ATTTAGCACA 
GAGGATGTTG TAGCATTAAA TAAAGAGGTG ACTGAAGTAC AGTTGAGTGC TACAGAAGAT 
AGTGCCACAG ATCCACAAAC CCCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

carf ( XM_012971179.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826633.2 Xenopus tropicalis calcium responsive transcription factor (carf), mRNA.

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CloneID	OXa46879
Clone ID Related Accession (Same CDS sequence)	XM_012971179.2
Accession Version	XM_012971179.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LOW QUALITY PROTEIN: calcium-responsive transcription factor
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971179.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAAAGTA ATGGACTGGA CGTTGATGTA CTGACTCGGC CTGCAGACTT GTTGATCACT 
TGTACCAATA CAGAGGATAA TGCTGATCTG GATGATGGGG TTCTCAAGAA TACTGCAAAC 
CAAATATCGA CCCTCACTGA GCAGTACCAT TTTATAGATC AAAGTGGAAA TACCATCCAC 
TATGCAATTC AGGCAGTGCC TGGTGCCAAC CCCCAAATGA TGATCCTTAC CAGTGAATCA 
GAAAATGGGC AAGTGTTCCA TGTCATTCCT TCCAGTCAAT CAGGAACAAC TCAGTTTGTT 
ATTCCACAGG GGCAGGACAT AGATGAGCAG CTGCAACAGA ATGAAAGCAC TGAAAATCTG 
CAGGTAGTAG CAGGCAACAC TGTGACAGAA AATGCAGCCA GCTTTATTAT TCATTCCAAT 
GCATCAATGG CCATATCAAA GATACCATCT GAGAGGTCAC CTACAGAGTT GTTCCAAAAA 
CCTCTCCTAC CACTGCCAAC AAACATTCCT GTTTGGGCTC GTCGTCTCAG GAACTGTGAG 
AAAATAGGAG ACTCTTACCG TGGTTACTGC TTAAGTGAGA ATGAGCTAAT GAGCATTTTA 
GCACTACACA AACAGCAAAC ACAGAGTGTT TGGGGAACCC GCCAGTCTCC TAGTCCTGCT 
AAACCAGCTA CAAGACTTAT GTGGAAGTCA CAGTATGTTC CATATGACGG AATTCCTTTT 
GTTAATGCAG GGAGCAGAGC AATCGTTATG GAATGTCAGT ATGGGCCACG AAGAAAAGGA 
GCCCAACCCA AAAAGAGCGC TGAGAATGAC AACATGTCTG GGGGACTGTA CAAAGCAACT 
TGTCCTGCAA GGATATACAT CAAAAAAGTA AAGAAGTTCC CTAATTACAG AGTGCCCACA 
GATCCAAAGA TTGACAAGAA ACTTATAAGG ATGGAACAGG AGAAAGCCTT TAATTTGTTA 
AAGAAGGATC TGGATGGGAC AGATGGAGTT CTCAGGTGGT ACATGCAGTT ACCAGATCAA 
GAAGCTCATC AGTATCATGA TCTGGAAACT GGTTGCTTTT CACCATCAAC TTTCCAGCCA 
TCCATTGAAG ATGAGGAGGA GATTCTGTGT GACGAGAACT CACAACCCTC ACGGCTACAT 
CCACAAGTAG CAGAAAAAAT CCGTGATCTG GTGTCCAAAG GTTTGAGTGA GGTTTACTCA 
GTTAGGAAAC AGCTTAGGAA ATTTGTGGAG CGGGATCTGT TTAAACCCGA TGACATACCT 
GAGCGCCATA ACTTATCCTA CTTTCCAACT GTGAATGATC TAAAAAATCA TATTCATGAG 
GCACAGAAAT CTTTGGCAAA CGAGGAGCTC ATTTATGATG CGGAAACAAT CCCTGAGCCA 
GTTAGAGAGA TCCAGTGGAC TGCTGACAGT GGAAACGTTC TTAATGAGAT GGTTACTGTA 
ACATTGGACC AATTCAACAG GAAGAACAAG AGACATTTGA AAGTGGAGAA TCCTCCCTCA 
GAGGATGTTT GCCAAGACAC AGCTCAGCTG ATCTCCTCCC TGACCACACT TCAGCCCAAA 
ATCTTTGCTC AGCTACAGGG ACTCCAGCTG CAACCGTCCT TAACATCAGT AGATGGAAAA 
ACTGCTCTTA TCACTGTGAA TCCACCAGGT CCAACCTTTC TACCGGCTGA AGGTAAAACT 
GCACTTATTA CTGTTAATCC CGTGGACCAA TCATTTACAA CTTCTGATGG GAAAACTGCA 
CTTTTTACTG TAAATCCCCC TGCTCCTTCA TTTACATCTG CTGATGGAAC TGCTGCACTT 
ATCACTGTGA ATTCTCCTGC TCCTTCATTT ACATCAGCGG ATGGGACAGC GGCATTGATC 
ACTGTGCATC CCTCTGCCAT AGGCAGTTCT ACATGCCTGT TGGGATCAGG AGGAAACAGT 
CAGCCTTTGG TGGTCAGTCA TTTGCAGAAT GACTCCCCTG TGTCCTCTGT CACTAGTTTA 
CCTGCCTCTG CCCACAACCT AGTCAGTATG AGCCAGATTG TAACAGTGGA AGAGGCAAGT 
GGAATGGCTG GAGAGGTGCA TCAAATTCTG CTTGGAGATA TCCACCCCAT TCCTATCAGG 
ATAGTGGAGA ACCAACAAAC AATTGTTGAA ATAAATGCTC CTAATGCATT TAGCACAGAG 
GATGTTGTAG CATTAAATAA AGAGGTGACT GAAGTACAGT TGAGTGCTAC AGAAGATAGC 
GCCACAGATC CACAAACCCC TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826633.2
CDS	94..2340
Translation	MESNGLDVDVLTRPADLLITCTNTEDNADLDDGVLKNTANQISTLTEQYHFIDQSGNTIHYAIQAVPGANPQMMILTSESENGQVFHVIPSSQSGTTQFVIPQGQDIDEQLQQNESTENLQVVAGNTVTENAASFIIHSNASMAISKIPSERSPTELFQKPLLPLPTNIPVWARRLRNCEKIGDSYRGYCLSENELMSILALHKQQTQSVWGTRQSPSPAKPATRLMWKSQYVPYDGIPFVNAGSRAIVMECQYGPRRKGAQPKKSAENDNMSGGLYKATCPARIYIKKVKKFPNYRVPTDPKIDKKLIRMEQEKAFNLLKKDLDGTDGVLRWYMQLPDQEAHQYHDLETGCFSPSTFQPSIEDEEEILCDENSQPSRLHPQVAEKIRDLVSKGLSEVYSVRKQLRKFVERDLFKPDDIPERHNLSYFPTVNDLKNHIHEAQKSLANEELIYDAETIPEPVREIQWTADSGNVLNEMVTVTLDQFNRKNKRHLKVENPPSEDVCQDTAQLISSLTTLQPKIFAQLQGLQLQPSLTSVDGKTALITVNPPGPTFLPAEGKTALITVNPVDQSFTTSDGKTALFTVNPPAPSFTSADGTAALITVNSPAPSFTSADGTAALITVHPSAIGSSTCLLGSGGNSQPLVVSHLQNDSPVSSVTSLPASAHNLVSMSQIVTVEEASGMAGEVHQILLGDIHPIPIRIVENQQTIVEINAPNAFSTEDVVALNKEVTEVQLSATEDSATDPQTPV

Target ORF information:

RefSeq Version	XM_012971179.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis calcium responsive transcription factor (carf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971179.2

ORF Insert Sequence:

ATGGAAAGTA ATGGACTGGA CGTTGATGTA CTGACTCGGC CTGCAGACTT GTTGATCACT 
TGTACCAATA CAGAGGATAA TGCTGATCTG GATGATGGGG TTCTCAAGAA TACTGCAAAC 
CAAATATCGA CCCTCACTGA GCAGTACCAT TTTATAGATC AAAGTGGAAA TACCATCCAC 
TATGCAATTC AGGCAGTGCC TGGTGCCAAC CCCCAAATGA TGATCCTTAC CAGTGAATCA 
GAAAATGGGC AAGTGTTCCA TGTCATTCCT TCCAGTCAAT CAGGAACAAC TCAGTTTGTT 
ATTCCACAGG GGCAGGACAT AGATGAGCAG CTGCAACAGA ATGAAAGCAC TGAAAATCTG 
CAGGTAGTAG CAGGCAACAC TGTGACAGAA AATGCAGCCA GCTTTATTAT TCATTCCAAT 
GCATCAATGG CCATATCAAA GATACCATCT GAGAGGTCAC CTACAGAGTT GTTCCAAAAA 
CCTCTCCTAC CACTGCCAAC AAACATTCCT GTTTGGGCTC GTCGTCTCAG GAACTGTGAG 
AAAATAGGAG ACTCTTACCG TGGTTACTGC TTAAGTGAGA ATGAGCTAAT GAGCATTTTA 
GCACTACACA AACAGCAAAC ACAGAGTGTT TGGGGAACCC GCCAGTCTCC TAGTCCTGCT 
AAACCAGCTA CAAGACTTAT GTGGAAGTCA CAGTATGTTC CATATGACGG AATTCCTTTT 
GTTAATGCAG GGAGCAGAGC AATCGTTATG GAATGTCAGT ATGGGCCACG AAGAAAAGGA 
GCCCAACCCA AAAAGAGCGC TGAGAATGAC AACATGTCTG GGGGACTGTA CAAAGCAACT 
TGTCCTGCAA GGATATACAT CAAAAAAGTA AAGAAGTTCC CTAATTACAG AGTGCCCACA 
GATCCAAAGA TTGACAAGAA ACTTATAAGG ATGGAACAGG AGAAAGCCTT TAATTTGTTA 
AAGAAGGATC TGGATGGGAC AGATGGAGTT CTCAGGTGGT ACATGCAGTT ACCAGATCAA 
GAAGCTCATC AGTATCATGA TCTGGAAACT GGTTGCTTTT CACCATCAAC TTTCCAGCCA 
TCCATTGAAG ATGAGGAGGA GATTCTGTGT GACGAGAACT CACAACCCTC ACGGCTACAT 
CCACAAGTAG CAGAAAAAAT CCGTGATCTG GTGTCCAAAG GTTTGAGTGA GGTTTACTCA 
GTTAGGAAAC AGCTTAGGAA ATTTGTGGAG CGGGATCTGT TTAAACCCGA TGACATACCT 
GAGCGCCATA ACTTATCCTA CTTTCCAACT GTGAATGATC TAAAAAATCA TATTCATGAG 
GCACAGAAAT CTTTGGCAAA CGAGGAGCTC ATTTATGATG CGGAAACAAT CCCTGAGCCA 
GTTAGAGAGA TCCAGTGGAC TGCTGACAGT GGAAACGTTC TTAATGAGAT GGTTACTGTA 
ACATTGGACC AATTCAACAG GAAGAACAAG AGACATTTGA AAGTGGAGAA TCCTCCCTCA 
GAGGATGTTT GCCAAGACAC AGCTCAGCTG ATCTCCTCCC TGACCACACT TCAGCCCAAA 
ATCTTTGCTC AGCTACAGGG ACTCCAGCTG CAACCGTCCT TAACATCAGT AGATGGAAAA 
ACTGCTCTTA TCACTGTGAA TCCACCAGGT CCAACCTTTC TACCGGCTGA AGGTAAAACT 
GCACTTATTA CTGTTAATCC CGTGGACCAA TCATTTACAA CTTCTGATGG GAAAACTGCA 
CTTTTTACTG TAAATCCCCC TGCTCCTTCA TTTACATCTG CTGATGGAAC TGCTGCACTT 
ATCACTGTGA ATTCTCCTGC TCCTTCATTT ACATCAGCGG ATGGGACAGC GGCATTGATC 
ACTGTGCATC CCTCTGCCAT AGGCAGTTCT ACATGCCTGT TGGGATCAGG AGGAAACAGT 
CAGCCTTTGG TGGTCAGTCA TTTGCAGAAT GACTCCCCTG TGTCCTCTGT CACTAGTTTA 
CCTGCCTCTG CCCACAACCT AGTCAGTATG AGCCAGATTG TAACAGTGGA AGAGGCAAGT 
GGAATGGCTG GAGAGGTGCA TCAAATTCTG CTTGGAGATA TCCACCCCAT TCCTATCAGG 
ATAGTGGAGA ACCAACAAAC AATTGTTGAA ATAAATGCTC CTAATGCATT TAGCACAGAG 
GATGTTGTAG CATTAAATAA AGAGGTGACT GAAGTACAGT TGAGTGCTAC AGAAGATAGC 
GCCACAGATC CACAAACCCC TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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carf cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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