nphs1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nphs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nphs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa40681 XM_018089423.1
Latest version!
Xenopus tropicalis nephrosis 1, congenital, Finnish type (nephrin) (nphs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OXa62093 XM_031905692.1
Latest version!
Xenopus tropicalis NPHS1 nephrin (nphs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa40681
    Clone ID Related Accession (Same CDS sequence) XM_018089423.1
    Accession Version XM_018089423.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nephrin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683384.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGCCTG TACTGTTTGC CGCTTGTTGC AGTTGGTGGA TCTTAAGGGA AGGCGTGACG 
    GCTAAGCAGC AGGTGTTCCG AGTGCAGCCG GACAATATCA CCGTCGTGCA GGGATCGACC
    GCACTGCTGA GTTGTGAGGT GGAACATGGG ACTGGTATGG TGCAGTGGGA AAAGAATGGA
    CTCCTGCTGG GACCTGACAG GAATATCCCA GGGTTCCTGC GCTACAGCAT GACTGGAGAC
    CCACACAAAG GAGAGTACAA CCTGCGCATT GAGCGTAGCG AGCTGGATGA CGACGCCGAG
    TATCACTGTC AGGTGGGGAG ATCAGAGGTC AGCTTGGGCA TCATTTCCCG CACTGCTCTC
    ATCAACGTCC TCATTCCTCC CAAAGCACCC ACTTTTGAAG AGTATGCTGC GGGCAGCCGA
    GTGACTTGGG TTTACGGGGT GGAATATACA GTCACCTGCC AAGCAACTGA CGCCAAACCG
    GCTGCTGCCT TGACCATCAA AAAGAGTGGA CTGGAGGTGT CTGGAGATTC ATCTTCAAAC
    CCAGGATCCA GAGACAAATT GGAGAACACA GCAATGGTGG CAAAGGTCAT CCCCCAGAGC
    TCTGATAATG GGAAACTGCT GACCTGCTTG GCGCTAAATC CAGCTCTTCA GACGCCAATC
    ACCGTGAGCT TCACCATGAA TGTGCTGTTT CCGCCTAATG CCCCGATTAT AGAAGGTTAT
    ATTGGGCCAT CGGTTAAAGC TGGGGAAACA CTGAAGCTCA TCTGCATTTC TTTAAGTGGA
    AACCCCCTGG CCACATTACA ATGGTCAAAG AACGATGATG TTGTGTCAAC ACGGTGGGAG
    ACGGATGAGG TGGTCCGAGC TTCTCGTAGT CACCTGACCC TGAATATTAA GCCTGCGGAT
    AACATGGCGG TTGTGAGCTG TGAGGCTGTA AATCATGTGA CTCCAGAGAC TCTCAAAACC
    AGCATCATCC TCAATGTTGT GTTTCTGCCA ACAGAAGTAA CCATCCTTGG CTCATCAAGT
    GGCCCAGAGA AATCTAATCT AACGTTCTCC TGCTTTACGG CTGCCAGTAA CCCTCCTGTA
    CAGATCCGCT GGTGGCTGGG CCCAAAGGAA CTAGTGAACA CCATTGTCAC CATCTCTGAT
    GCTGTTCATG GTGGAAAGGT GACAATGTCC AACCTCACTA TGGAGGCTCT TCGAGAGGAG
    CATGGAATGG CGTTGACCTG TGAGGCCTTT AATGAGGCCA TTCTCTACAC CAAATCCAAC
    AGCGTTACCC TAAGTGTGCA ATACCCTCCA CAGAATGTCT GGATCGAGGC CCCTCCAGCT
    GATAAGTTCT TCCGTGCGGG GACATCAGTG AAGATGACTT GTTTCTCTAG CGGTGGAAAC
    CCGCCACCCA GGCTTACCTG GATAAAGGAT ACTAAAAGCC TGAGTGGGGG ATCGCAGATC
    CATTCAGGAA AAATTGTCAC AAAAGAAATT ACAATTATAA CTGTGCCAAG TGACAATATG
    GCCACATACC GGTGCAACGC CAGCAACATT GGGAAGACGC CGGCGCTGAC TGCCAGCACG
    AAACTAAAGG TCCAGTTTCC TGCCATAGAT GTGAATATAA TTAGCAGCGC CAAAGAGTAT
    CGCCGCGGTT CTACCATTAT ACTGACCTGT GTCACCGGCA GCAGTAACCC TGCATCTACT
    ATATCCTGGG TGAAAGATGG AGAGCAGTTA AAAGCTCAGG ATTTGGGAAG AAAACCTTCT
    CTGTATGGGG GAATTTCCAG TTCCAGTCGT GTCACACTGA AAGCAGCATC TAAGGACAAC
    GGAAGGCGCG TCTCCTGCGA GGCGTTCAGC AGCGTCCTCA ACGAGGCCGT CAACACCTTC
    TACAAACTCA ATATCCTGTT TCCCCCTGAG TTTCTGGATG ATCAGCCGGC GGTGGTACAG
    GCCGTGGAGC ATGGAGCTGC CCTTATCCCA GTCCGGGTCA GGGCCAACCC ACCTCAGATC
    AACTACACGT GGAGCCTTTG GGGCGAGAAA CTGATCCGAG ATGGTGCTTA CCGACATCAC
    CTGCGCGGTG AGGGGGCCCT CGAGATATGG AACGTGAGTC GCGCTGACTC CGGGGTCTAC
    AACGTGACTT GTGTGAATAA AGAGGGGAAG AACAGCATTG TAATAAGACT GGATGTGCAG
    TATTCTCCCA CCATTCGAAG CCTTGGGGAC ACAGAGGTGG ATCTCGGTTC AGACGCAGAG
    ATCGCTTGCA CGGCAGATGC GAATCCAGCG ACAGACTCAA TGTTCCAGTG GAGATGGCTG
    GTCTGGATCC GTTTATATTG CTCTGTGATC ATTACCATTA GACCTTATGT TGGTTTCCAG
    TTAAAGGGGC TCTTTTACCA ATGCGAGTGC AAAGCGCTAC AATGGATACA AAATGGCGCA
    TATCTGGATG TAGTTAAGCC TGATATACAC AAGGGGGTGC ACTTGAGCAA AGTGGCAGTT
    CCTGGTGATG GGAAGAGCGT GGCAGCCTTG GTGTGTAAAG CCGAGGGAAT CCCGAGTGTG
    GCGTTTAGCT GGGCTAAGAA TGGGGTATCT CTGGATCTCA AAGATCCCAG GTACTCAGAG
    AAGACATTTC ATGAGCTGTC AGTTCACACC AGCACGCTGG TCATTTCCAA TGTGAGCGCA
    GTGAAGGACT ACGCCCTCTT CACGTGTACG GCCACGAACA CCCTGGGGGT CGACAGTTTC
    ACCATTCAGC TTGTCAGCCC CAGCCGTCCT GACCCTCCTT CAGGTCTGAG AGTCATCGGT
    TTCACTCACA ACTGGGTGAC GCTACAATGG AGTGCTGGAT TTGATGGAGG GGCGGAGCAA
    AAATTTCGAG TCAGATCCTT TTTTTTTTTT TTTAAACTTT TTTACCCAGA AGCCCTTGAG
    TGGCCACCAT ACATATTAGC AGCTGTGTGT GCCGGAGGTG CCCTCCTTCT GGTCTCCAAT
    GCGGCTTTTA TTGGGTGCCT CGTGAAAAGA AGCAGAGACA AGTCCCAAGG AGGAGAAGGT
    GTGAAGAAGG ATGAGAGGAA TGTTTCTCTA TGTTTGCGCT TCTCAACCAA ACAGGGCGGC
    TATGAAGACT GGTATTCGAC TTCCGAAGGC CATGAGTATG AAGAAGTAGG AGATTACCCA
    CAATCCTCTA GAGTCCGATG GCAGAACAGA GAAACGGCCA ATCAGGGAAG AGAGGCATCG
    TGGGCAGAAT ACCAGGATCC AGGCAGATTA AGAATCTATG ACGAGGCAGA CGTGACCTTG
    TCTGACGTGT ACGAGACTCC ATATGGGGGG CGGGGGCAAC TAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017944912.1
    CDS166..3453
    Translation

    Target ORF information:

    RefSeq Version XM_018089423.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nephrosis 1, congenital, Finnish type (nephrin) (nphs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089423.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGCCTG TACTGTTTGC CGCTTGTTGC AGTTGGTGGA TCTTAAGGGA AGGCGTGACG 
    GCTAAGCAGC AGGTGTTCCG AGTGCAGCCG GACAATATCA CCGTCGTGCA GGGATCGACC
    GCACTGCTGA GTTGTGAGGT GGAACATGGG ACTGGTATGG TGCAGTGGGA AAAGAATGGA
    CTCCTGCTGG GACCTGACAG GAATATCCCA GGGTTCCTGC GCTACAGCAT GACTGGAGAC
    CCACACAAAG GAGAGTACAA CCTGCGCATT GAGCGTAGCG AGCTGGATGA CGACGCCGAG
    TATCACTGTC AGGTGGGGAG ATCAGAGGTC AGCTTGGGCA TCATTTCCCG CACTGCTCTC
    ATCAACGTCC TCATTCCTCC CAAAGCACCC ACTTTTGAAG AGTATGCTGC GGGCAGCCGA
    GTGACTTGGG TTTACGGGGT GGAATATACA GTCACCTGCC AAGCAACTGA CGCCAAACCG
    GCTGCTGCCT TGACCATCAA AAAGAGTGGA CTGGAGGTGT CTGGAGATTC ATCTTCAAAC
    CCAGGATCCA GAGACAAATT GGAGAACACA GCAATGGTGG CAAAGGTCAT CCCCCAGAGC
    TCTGATAATG GGAAACTGCT GACCTGCTTG GCGCTAAATC CAGCTCTTCA GACGCCAATC
    ACCGTGAGCT TCACCATGAA TGTGCTGTTT CCGCCTAATG CCCCGATTAT AGAAGGTTAT
    ATTGGGCCAT CGGTTAAAGC TGGGGAAACA CTGAAGCTCA TCTGCATTTC TTTAAGTGGA
    AACCCCCTGG CCACATTACA ATGGTCAAAG AACGATGATG TTGTGTCAAC ACGGTGGGAG
    ACGGATGAGG TGGTCCGAGC TTCTCGTAGT CACCTGACCC TGAATATTAA GCCTGCGGAT
    AACATGGCGG TTGTGAGCTG TGAGGCTGTA AATCATGTGA CTCCAGAGAC TCTCAAAACC
    AGCATCATCC TCAATGTTGT GTTTCTGCCA ACAGAAGTAA CCATCCTTGG CTCATCAAGT
    GGCCCAGAGA AATCTAATCT AACGTTCTCC TGCTTTACGG CTGCCAGTAA CCCTCCTGTA
    CAGATCCGCT GGTGGCTGGG CCCAAAGGAA CTAGTGAACA CCATTGTCAC CATCTCTGAT
    GCTGTTCATG GTGGAAAGGT GACAATGTCC AACCTCACTA TGGAGGCTCT TCGAGAGGAG
    CATGGAATGG CGTTGACCTG TGAGGCCTTT AATGAGGCCA TTCTCTACAC CAAATCCAAC
    AGCGTTACCC TAAGTGTGCA ATACCCTCCA CAGAATGTCT GGATCGAGGC CCCTCCAGCT
    GATAAGTTCT TCCGTGCGGG GACATCAGTG AAGATGACTT GTTTCTCTAG CGGTGGAAAC
    CCGCCACCCA GGCTTACCTG GATAAAGGAT ACTAAAAGCC TGAGTGGGGG ATCGCAGATC
    CATTCAGGAA AAATTGTCAC AAAAGAAATT ACAATTATAA CTGTGCCAAG TGACAATATG
    GCCACATACC GGTGCAACGC CAGCAACATT GGGAAGACGC CGGCGCTGAC TGCCAGCACG
    AAACTAAAGG TCCAGTTTCC TGCCATAGAT GTGAATATAA TTAGCAGCGC CAAAGAGTAT
    CGCCGCGGTT CTACCATTAT ACTGACCTGT GTCACCGGCA GCAGTAACCC TGCATCTACT
    ATATCCTGGG TGAAAGATGG AGAGCAGTTA AAAGCTCAGG ATTTGGGAAG AAAACCTTCT
    CTGTATGGGG GAATTTCCAG TTCCAGTCGT GTCACACTGA AAGCAGCATC TAAGGACAAC
    GGAAGGCGCG TCTCCTGCGA GGCGTTCAGC AGCGTCCTCA ACGAGGCCGT CAACACCTTC
    TACAAACTCA ATATCCTGTT TCCCCCTGAG TTTCTGGATG ATCAGCCGGC GGTGGTACAG
    GCCGTGGAGC ATGGAGCTGC CCTTATCCCA GTCCGGGTCA GGGCCAACCC ACCTCAGATC
    AACTACACGT GGAGCCTTTG GGGCGAGAAA CTGATCCGAG ATGGTGCTTA CCGACATCAC
    CTGCGCGGTG AGGGGGCCCT CGAGATATGG AACGTGAGTC GCGCTGACTC CGGGGTCTAC
    AACGTGACTT GTGTGAATAA AGAGGGGAAG AACAGCATTG TAATAAGACT GGATGTGCAG
    TATTCTCCCA CCATTCGAAG CCTTGGGGAC ACAGAGGTGG ATCTCGGTTC AGACGCAGAG
    ATCGCTTGCA CGGCAGATGC GAATCCAGCG ACAGACTCAA TGTTCCAGTG GAGATGGCTG
    GTCTGGATCC GTTTATATTG CTCTGTGATC ATTACCATTA GACCTTATGT TGGTTTCCAG
    TTAAAGGGGC TCTTTTACCA ATGCGAGTGC AAAGCGCTAC AATGGATACA AAATGGCGCA
    TATCTGGATG TAGTTAAGCC TGATATACAC AAGGGGGTGC ACTTGAGCAA AGTGGCAGTT
    CCTGGTGATG GGAAGAGCGT GGCAGCCTTG GTGTGTAAAG CCGAGGGAAT CCCGAGTGTG
    GCGTTTAGCT GGGCTAAGAA TGGGGTATCT CTGGATCTCA AAGATCCCAG GTACTCAGAG
    AAGACATTTC ATGAGCTGTC AGTTCACACC AGCACGCTGG TCATTTCCAA TGTGAGCGCA
    GTGAAGGACT ACGCCCTCTT CACGTGTACG GCCACGAACA CCCTGGGGGT CGACAGTTTC
    ACCATTCAGC TTGTCAGCCC CAGCCGTCCT GACCCTCCTT CAGGTCTGAG AGTCATCGGT
    TTCACTCACA ACTGGGTGAC GCTACAATGG AGTGCTGGAT TTGATGGAGG GGCGGAGCAA
    AAATTTCGAG TCAGATCCTT TTTTTTTTTT TTTAAACTTT TTTACCCAGA AGCCCTTGAG
    TGGCCACCAT ACATATTAGC AGCTGTGTGT GCCGGAGGTG CCCTCCTTCT GGTCTCCAAT
    GCGGCTTTTA TTGGGTGCCT CGTGAAAAGA AGCAGAGACA AGTCCCAAGG AGGAGAAGGT
    GTGAAGAAGG ATGAGAGGAA TGTTTCTCTA TGTTTGCGCT TCTCAACCAA ACAGGGCGGC
    TATGAAGACT GGTATTCGAC TTCCGAAGGC CATGAGTATG AAGAAGTAGG AGATTACCCA
    CAATCCTCTA GAGTCCGATG GCAGAACAGA GAAACGGCCA ATCAGGGAAG AGAGGCATCG
    TGGGCAGAAT ACCAGGATCC AGGCAGATTA AGAATCTATG ACGAGGCAGA CGTGACCTTG
    TCTGACGTGT ACGAGACTCC ATATGGGGGG CGGGGGCAAC TAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62093
    Clone ID Related Accession (Same CDS sequence) XM_031905692.1
    Accession Version XM_031905692.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nephrin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGCCTG TACTGTTTGC CGCTTGTTGC AGTTGGTGGA TCTTAAGGGA AGGCGTGACG 
    GCTAAGCAGC AGGTGTTCCG AGTGCAGCCG GACAATATCA CCGTCGTGCA GGGATCGACC
    GCACTGCTGA GTTGTGAGGT GGAACATGGG ACTGGTATGG TGCAGTGGGA AAAGAATGGA
    CTCCTGCTGG GACCTGACAG GAATATCCCA GGGTTCCTGC GCTACAGCAT GACTGGAGAC
    CCACACAAAG GAGAGTACAA CCTGCGCATT GAGCGTAGCG AGCTGGATGA CGACGCCGAG
    TATCACTGTC AGGTGGGGAG ATCAGAGGTC AGCTTGGGCA TCATTTCCCG CACTGCTCTC
    ATCAACGTCC TCATTCCTCC CAAAGCACCC ACTTTTGAAG AGTATGCTGC GGGCAGCCGA
    GTGACTTGGG TTTACGGGGT GGAATATACA GTCACCTGCC AAGCAACTGA CGCCAAACCG
    GCTGCTGCCT TGACCATCAA AAAGAGTGGA CTGGAGGTGT CTGGAGATTC ATCTTCAAAC
    CCAGGATCCA GAGACAAATT GGAGAACACA GCAATGGTGG CAAAGGTCAT CCCCCAGAGC
    TCTGATAATG GGAAACTGCT GACCTGCTTG GCGCTAAATC CAGCTCTTCA GACGCCAATC
    ACCGTGAGCT TCACCATGAA TGTGCTGTTT CCGCCTAATG CCCCGATTAT AGAAGGTTAT
    ATTGGGCCAT CGGTTAAAGC TGGGGAAACA CTGAAGCTCA TCTGCATTTC TTTAAGTGGA
    AACCCCCTGG CCACATTACA ATGGTCAAAG AACGATGATG TTGTGTCAAC ACGGTGGGAG
    ACGGATGAGG TGGTCCGAGC TTCTCGTAGT CACCTGACCC TGAATATTAA GCCTGCGGAT
    AACATGGCGG TTGTGAGCTG TGAGGCTGTA AATCATGTGA CTCCAGAGAC TCTCAAAACC
    AGCATCATCC TCAATGTTGT GTTTCTGCCA ACAGAAGTAA CCATCCTTGG CTCATCAAGT
    GGCCCAGAGA AATCTAATCT AACGTTCTCC TGCTTTACGG CTGCCAGTAA CCCTCCTGTA
    CAGATCCGCT GGTGGCTGGG CCCAAAGGAA CTAGTGAACA CCATTGTCAC CATCTCTGAT
    GCTGTTCATG GTGGAAAGGT GACAATGTCC AACCTCACTA TGGAGGCTCT TCGAGAGGAG
    CATGGAATGG CGTTGACCTG TGAGGCCTTT AATGAGGCCA TTCTCTACAC CAAATCCAAC
    AGCGTTACCC TAAGTGTGCA ATACCCTCCA CAGAATGTCT GGATCGAGGC CCCTCCAGCT
    GATAAGTTCT TCCGTGCGGG GACATCAGTG AAGATGACTT GTTTCTCTAG CGGTGGAAAC
    CCGCCACCCA GGCTTACCTG GATAAAGGAT ACTAAAAGCC TGAGTGGGGG ATCGCAGATC
    CATTCAGGAA AAATTGTCAC AAAAGAAATT ACAATTATAA CTGTGCCAAG TGACAATATG
    GCCACATACC GGTGCAACGC CAGCAACATT GGGAAGACGC CGGCGCTGAC TGCCAGCACG
    AAACTAAAGG TCCAGTTTCC TGCCATAGAT GTGAATATAA TTAGCAGCGC CAAAGAGTAT
    CGCCGCGGTT CTACCATTAT ACTGACCTGT GTCACCGGCA GCAGTAACCC TGCATCTACT
    ATATCCTGGG TGAAAGATGG AGAGCAGTTA AAAGCTCAGG ATTTGGGAAG AAAACCATCT
    CTGTATGGGG GAATTTCCAG TTCCAGTCGT GTCACACTGA AAGCAGCATC TAAGGACAAC
    GGAAGGCGCG TCTCCTGCGA GGCGTTCAGC AGCGTCCTCA ACGAGGCCGT CAACACCTTC
    TACAAACTCA ATATCCTGTT TCCCCCTGAG TTTCTGGATG ATCAGCCGGC GGTGGTGCAG
    GCCGTGGAGC ATGGAGCTGC CCTTATCCCA GTCCGGGTCA GGGCCAACCC ACCTCAGATC
    AACTACACGT GGAGCCTTTG GGGCGAGAAA CTGATCCGAG ATGGTGCTTA CCGACATCAC
    CTGCGCGGTG AGGGGGCCCT CGAGATATGG AACGTGAGTC GCGCTGACTC CGGGGTCTAC
    AATGTGACTT GTGTGAATAA AGAGGGGAAG AACAGCATTG TAATAAGACT GGATGTGCAG
    TATTCTCCCA CCATTCGAAG CCTTGGGGAC ACAGAGGTGG ATCTCGGTTC AGACGCAGAG
    ATCGCTTGCA CGGCAGATGC GAATCCAGCG ACAGACTCAA TGTTCCAGTG GAGATGGCTG
    GGTGATGAGG AGCGAGACCT CTCTGCCTTG GAACGCAAAG TGGATGGAGT CATTGGACGC
    CTGGTTATCC GGGAAGCCAA GAGAACGGAC GCCGGCCGCT ACGAGTGCAC CGTGGATAAC
    GGGATCCCCC CCTCCATCAA GGCTGATGCT CGTCTGATTG TCCGATTTAA GCCTGATATA
    CACAAGGGGG TGCACTTGAG CAAAGTGGCA GTTCCTGGTG ATGGGAAGAG CGTGGCAGCC
    TTGGTGTGTA AAGCTGAGGG AATCCCGAGT GTGGCGTTTA GCTGGGCTAA GAATGGGGTA
    TCTCTGGATC TCAAAGATCC CAGGTACTCA GAGAAGACAT TTCATGAGCT GTCAGTTCAC
    ACCAGCACGC TGGTCATTTC CAATGTGAGC GCAGTGAAGG ACTACGCCCT CTTCACGTGT
    ACGGCCACGA ACACCCTGGG GGTCGACAGT TTCACCATTC AGCTTGTCAG CACCAGCCGT
    CCTGACCCTC CTTCAGGTCT GAGAGTCATC GGTTTCACTC ACAACTCGGT GACGCTACAA
    TGGAGTGCTG GATTTGATGG AGGGGCGGAG CAAAAATTTC GAGTCAGGTA CCGCTGGCCC
    GGCACCGACA GCCACATGTA TGTAGATGTA TTCCCACCCC AGGAAACCGT ATTCACCATC
    ACAGGCCTGA AAGGAAGCAC CGCCTACAAC TTCTCAGTCA ATGCTATCAA TGCCTTGGGG
    GAAAGCGACT ACGCAGACCA GGGGGCGGTG GTTAGTGTCA CCACAAAAGA GACGACGTTG
    CTTCCGGTAC CTACTCAGCA GCCAACCCGT GCACCCACAG CAGAAGCCCT TGAGTGGCCA
    CCATACATAT TAGCAGCTGT GTGTGCCGGA GGTGCCCTCC TTCTGGTCTC CAATGCGGCT
    TTTATTGGGT GCCTCGTGAA AAGAAGCAGA GACAAGTCCC AAGGAGGAGA AGGTGTGAAG
    AAGGATGAGA GCCAGCAACG CGCACTTAAT GAATACGGAG ACGGGGAGCT CATCAACACC
    CACGCCAAGA AGACTCTACT GATTGACAGC GGTTCCGAGA CAGGTTCTAG TCTTTACCAA
    GACAGCGCTT CAGAATCTGG AATATATTAC TACCCAGTTA GAGATTACAG ACCTTCGCTT
    TCCCCTCAAT ATGAGGTTCC AGAGAGAGGA GAATCAGGGA TCCGAGGTTG GGAAGAGGGC
    GGCTATGAAG ACTGGTATTC GACTTCCGAA GGCCACGAGT ATGAAGAAGT AGGAGATTAC
    CCACAATCCT CTAGAGTCCG ATGGCAGAAC AGAGAAACGG CCAATCAGGG AAGAGAGGCA
    TCGTGGGCAG AATACCAGGA TCCAGGCAGA TTAAGAATCT ATGACGAGGC AGACGTGACC
    TTGTCTGACG TGTACGAGAC TCCGTATGGG GGGCGGGGGC AACTAGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761552.1
    CDS194..3904
    Translation

    Target ORF information:

    RefSeq Version XM_031905692.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis NPHS1 nephrin (nphs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTGCCTG TACTGTTTGC CGCTTGTTGC AGTTGGTGGA TCTTAAGGGA AGGCGTGACG 
    GCTAAGCAGC AGGTGTTCCG AGTGCAGCCG GACAATATCA CCGTCGTGCA GGGATCGACC
    GCACTGCTGA GTTGTGAGGT GGAACATGGG ACTGGTATGG TGCAGTGGGA AAAGAATGGA
    CTCCTGCTGG GACCTGACAG GAATATCCCA GGGTTCCTGC GCTACAGCAT GACTGGAGAC
    CCACACAAAG GAGAGTACAA CCTGCGCATT GAGCGTAGCG AGCTGGATGA CGACGCCGAG
    TATCACTGTC AGGTGGGGAG ATCAGAGGTC AGCTTGGGCA TCATTTCCCG CACTGCTCTC
    ATCAACGTCC TCATTCCTCC CAAAGCACCC ACTTTTGAAG AGTATGCTGC GGGCAGCCGA
    GTGACTTGGG TTTACGGGGT GGAATATACA GTCACCTGCC AAGCAACTGA CGCCAAACCG
    GCTGCTGCCT TGACCATCAA AAAGAGTGGA CTGGAGGTGT CTGGAGATTC ATCTTCAAAC
    CCAGGATCCA GAGACAAATT GGAGAACACA GCAATGGTGG CAAAGGTCAT CCCCCAGAGC
    TCTGATAATG GGAAACTGCT GACCTGCTTG GCGCTAAATC CAGCTCTTCA GACGCCAATC
    ACCGTGAGCT TCACCATGAA TGTGCTGTTT CCGCCTAATG CCCCGATTAT AGAAGGTTAT
    ATTGGGCCAT CGGTTAAAGC TGGGGAAACA CTGAAGCTCA TCTGCATTTC TTTAAGTGGA
    AACCCCCTGG CCACATTACA ATGGTCAAAG AACGATGATG TTGTGTCAAC ACGGTGGGAG
    ACGGATGAGG TGGTCCGAGC TTCTCGTAGT CACCTGACCC TGAATATTAA GCCTGCGGAT
    AACATGGCGG TTGTGAGCTG TGAGGCTGTA AATCATGTGA CTCCAGAGAC TCTCAAAACC
    AGCATCATCC TCAATGTTGT GTTTCTGCCA ACAGAAGTAA CCATCCTTGG CTCATCAAGT
    GGCCCAGAGA AATCTAATCT AACGTTCTCC TGCTTTACGG CTGCCAGTAA CCCTCCTGTA
    CAGATCCGCT GGTGGCTGGG CCCAAAGGAA CTAGTGAACA CCATTGTCAC CATCTCTGAT
    GCTGTTCATG GTGGAAAGGT GACAATGTCC AACCTCACTA TGGAGGCTCT TCGAGAGGAG
    CATGGAATGG CGTTGACCTG TGAGGCCTTT AATGAGGCCA TTCTCTACAC CAAATCCAAC
    AGCGTTACCC TAAGTGTGCA ATACCCTCCA CAGAATGTCT GGATCGAGGC CCCTCCAGCT
    GATAAGTTCT TCCGTGCGGG GACATCAGTG AAGATGACTT GTTTCTCTAG CGGTGGAAAC
    CCGCCACCCA GGCTTACCTG GATAAAGGAT ACTAAAAGCC TGAGTGGGGG ATCGCAGATC
    CATTCAGGAA AAATTGTCAC AAAAGAAATT ACAATTATAA CTGTGCCAAG TGACAATATG
    GCCACATACC GGTGCAACGC CAGCAACATT GGGAAGACGC CGGCGCTGAC TGCCAGCACG
    AAACTAAAGG TCCAGTTTCC TGCCATAGAT GTGAATATAA TTAGCAGCGC CAAAGAGTAT
    CGCCGCGGTT CTACCATTAT ACTGACCTGT GTCACCGGCA GCAGTAACCC TGCATCTACT
    ATATCCTGGG TGAAAGATGG AGAGCAGTTA AAAGCTCAGG ATTTGGGAAG AAAACCATCT
    CTGTATGGGG GAATTTCCAG TTCCAGTCGT GTCACACTGA AAGCAGCATC TAAGGACAAC
    GGAAGGCGCG TCTCCTGCGA GGCGTTCAGC AGCGTCCTCA ACGAGGCCGT CAACACCTTC
    TACAAACTCA ATATCCTGTT TCCCCCTGAG TTTCTGGATG ATCAGCCGGC GGTGGTGCAG
    GCCGTGGAGC ATGGAGCTGC CCTTATCCCA GTCCGGGTCA GGGCCAACCC ACCTCAGATC
    AACTACACGT GGAGCCTTTG GGGCGAGAAA CTGATCCGAG ATGGTGCTTA CCGACATCAC
    CTGCGCGGTG AGGGGGCCCT CGAGATATGG AACGTGAGTC GCGCTGACTC CGGGGTCTAC
    AATGTGACTT GTGTGAATAA AGAGGGGAAG AACAGCATTG TAATAAGACT GGATGTGCAG
    TATTCTCCCA CCATTCGAAG CCTTGGGGAC ACAGAGGTGG ATCTCGGTTC AGACGCAGAG
    ATCGCTTGCA CGGCAGATGC GAATCCAGCG ACAGACTCAA TGTTCCAGTG GAGATGGCTG
    GGTGATGAGG AGCGAGACCT CTCTGCCTTG GAACGCAAAG TGGATGGAGT CATTGGACGC
    CTGGTTATCC GGGAAGCCAA GAGAACGGAC GCCGGCCGCT ACGAGTGCAC CGTGGATAAC
    GGGATCCCCC CCTCCATCAA GGCTGATGCT CGTCTGATTG TCCGATTTAA GCCTGATATA
    CACAAGGGGG TGCACTTGAG CAAAGTGGCA GTTCCTGGTG ATGGGAAGAG CGTGGCAGCC
    TTGGTGTGTA AAGCTGAGGG AATCCCGAGT GTGGCGTTTA GCTGGGCTAA GAATGGGGTA
    TCTCTGGATC TCAAAGATCC CAGGTACTCA GAGAAGACAT TTCATGAGCT GTCAGTTCAC
    ACCAGCACGC TGGTCATTTC CAATGTGAGC GCAGTGAAGG ACTACGCCCT CTTCACGTGT
    ACGGCCACGA ACACCCTGGG GGTCGACAGT TTCACCATTC AGCTTGTCAG CACCAGCCGT
    CCTGACCCTC CTTCAGGTCT GAGAGTCATC GGTTTCACTC ACAACTCGGT GACGCTACAA
    TGGAGTGCTG GATTTGATGG AGGGGCGGAG CAAAAATTTC GAGTCAGGTA CCGCTGGCCC
    GGCACCGACA GCCACATGTA TGTAGATGTA TTCCCACCCC AGGAAACCGT ATTCACCATC
    ACAGGCCTGA AAGGAAGCAC CGCCTACAAC TTCTCAGTCA ATGCTATCAA TGCCTTGGGG
    GAAAGCGACT ACGCAGACCA GGGGGCGGTG GTTAGTGTCA CCACAAAAGA GACGACGTTG
    CTTCCGGTAC CTACTCAGCA GCCAACCCGT GCACCCACAG CAGAAGCCCT TGAGTGGCCA
    CCATACATAT TAGCAGCTGT GTGTGCCGGA GGTGCCCTCC TTCTGGTCTC CAATGCGGCT
    TTTATTGGGT GCCTCGTGAA AAGAAGCAGA GACAAGTCCC AAGGAGGAGA AGGTGTGAAG
    AAGGATGAGA GCCAGCAACG CGCACTTAAT GAATACGGAG ACGGGGAGCT CATCAACACC
    CACGCCAAGA AGACTCTACT GATTGACAGC GGTTCCGAGA CAGGTTCTAG TCTTTACCAA
    GACAGCGCTT CAGAATCTGG AATATATTAC TACCCAGTTA GAGATTACAG ACCTTCGCTT
    TCCCCTCAAT ATGAGGTTCC AGAGAGAGGA GAATCAGGGA TCCGAGGTTG GGAAGAGGGC
    GGCTATGAAG ACTGGTATTC GACTTCCGAA GGCCACGAGT ATGAAGAAGT AGGAGATTAC
    CCACAATCCT CTAGAGTCCG ATGGCAGAAC AGAGAAACGG CCAATCAGGG AAGAGAGGCA
    TCGTGGGCAG AATACCAGGA TCCAGGCAGA TTAAGAATCT ATGACGAGGC AGACGTGACC
    TTGTCTGACG TGTACGAGAC TCCGTATGGG GGGCGGGGGC AACTAGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.