asap2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	asap2
Entrez Gene ID	100494274
Full Name	ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Synonyms	ddef2
General protein information	Preferred Names ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 Names arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004914478.3	XP_004914535.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X2
XM_012963462.3	XP_012818916.2	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X3
XM_004914478.4	XP_004914535.2	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X2
XM_002935032.4	XP_002935078.2	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X1
XM_002935032.5	XP_002935078.3	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X1
XM_012963462.2	XP_012818916.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ASAP2	XP_515290.3
Canis lupus familiaris (dog)	ASAP2	XP_005630157.1
Mus musculus (house mouse)	Asap2	NP_001128664.1
Rattus norvegicus (Norway rat)	Asap2	XP_006240060.1
Gallus gallus (chicken)	ASAP2	XP_001231632.3
Xenopus tropicalis (tropical clawed frog)	asap2	XP_002935078.2
Homo sapiens (human)	ASAP2	NP_003878.1
	ASAP2	XP_001088227.2
Bos taurus (cattle)	ASAP2	XP_002691576.2
Danio rerio (zebrafish)	asap2a	NP_001038672.1

Gene Ontology

The following asap2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asap2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa35919	XM_012963462.2 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa59216	XM_012963462.3 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa59214	XM_002935032.5 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59215	XM_004914478.4 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa10335	XM_002935032.4 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa10336	XM_004914478.3 Latest version!	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

asap2 ( XM_012963462.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818916.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa35919
Clone ID Related Accession (Same CDS sequence)	XM_012963462.2
Accession Version	XM_012963462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGATC TGATCAGTGT GTCGGAATTT GTGTGTGAGA CCAATGAGGA TTACAAGTCC 
CCCACTGCCT CCAATTTCAC CAACAGGCTG AGCCAGTGCA GGAACACGGT CGTGGCTATA 
GATGAGGCTC TGGAGGTTGA TCACACGATT CTGAACAAAA TGAAGAAATC AGTCAAAGCC 
ATTCACACTT CTGGTCTGAC CCACGTGGAA AATGAGGAGC AGTACACTCA AGCACTGGAA 
AAATTTGGAA GCAACTGTGT TTGTCGGGAT GACCCTGATT TGGGAACAGC CTTCTTAAAA 
TTCTCCGTCT TTACCAAGGA GCTAACAGCA CTTTTCAAGA ACCTGGTACA GAACATGAAT 
AACATTATCA CATTTCCACT GGACAGCTTA CTGAAGGGAG ATCTTAAAGG GGTAAAAGGG 
GAATTGAAAA AGCCATTCGA AAAAGCCTGG AAAGACTATG AAACTAAAGT GACCAAAATT 
GAAAAGGAGA AGAAGGAGCA CGCCAAGATG CATGGTATGA TTCGCACTGA GATCAGTGGG 
GCTGAAATTG CTGAGGAAAT GGAGAAAGAA AGACGGGTTT TCCAGCTCCA AATGTGTGAG 
TACCTGCTGA AAGTGAATGA AATAAAGATT AAAAAAGGAG TAGATCTGCT GCAGAACCTG 
ATTAAGTACT TCCACGCACA GTGCAACTTT TTCCAGGATG GATTGAAAGC TGTTGAGACT 
CTGAAGCCCT CTATTGAAAA ACTTTCAACC GATCTGCACA CGATCAAGCA AGTCCAGGAT 
GAAGAGAGGA AGCAGCTTAG CCAGCTAAGG GATATCTTAA AAGCTGCCCT ACAAGTGGAG 
CAGAAGGAGG ATTCACAGAT CCGACAGAGC GTCGCTTACA GCTTGCATCA GCCACAGGGA 
AACAAGGAAC ACGGCTCTGA GAGGGACGGC TATCTCTATA AGAAGAGTGA TGGAATCAGA 
AAGGTTTGGC AGAAGAGGAA ATGCACAGTA CAAAATGGCT TTCTAACCAT ATCTCATGGC 
ACAGCAAACA TTCCGCCTGC AAAGCTGAAC CTCTTAACCT GTCAAGTGAA AACCAACCCA 
GAGGAAAAGA AGAGCTTTGA CCTTATCTCA CATGATCGGA CATATCACTT TCAAGCAGAG 
GATGAAACAG AGTGCCAAAT ATGGATGTCT GTGCTTCAGA ACAGCAAGGA AGAAGCCTTA 
AACAATGCAT TCAAGGGAGA CCACAGTGCA GGGGAGAACA ACATTGTTCA GGAACTCACT 
AAGGAAATAA TATCTGATAT CCAGAGAATG CCAGGGAACG ACGTGTGCTG TGACTGTGGT 
GCATCAGATC CAACTTGGCT TTCCACAAAC CTGGGGATCC TTACATGCAT TGAGTGCTCT 
GGGATACACA GAGAGCTGGG CGTGCATTAC TCCAGAATAC AGTCTCTTAC ACTGGATGTC 
TTGGGAACTT CTGAGCTTTT GCTTGCCAAA AACATTGGGA ACTCAGGTTT TAATGAACTA 
ATGGAAGCCT GTCTGCCTGC TGATGATTCC GTGAAGCCTT CACCTTGCAG TGACATGAAT 
GCACGGAAAG ACTATATCAC CACCAAGTAC ATAGAAAGAA AGTACGCAAG GAGAAAATAC 
CATGACAATG CCTCTAAACT GCATGGCTTG TGTGACGCCG TCAAATCCAG AGACATCTTT 
ACACTGGTAC AGTTACATGC TGAAGGGGTG GATCTCACCG AGGGCATACC ACTTGCCAAT 
GGACACGAAC AAGGTGAGAC TGCACTCCAT CTCGCTGTCA GATCAGTTGA CCGCAATTCC 
TTGCACATAG TCGACTTTCT TGTACAGAAT AGTGGCAACT TGGACAAACA GACAGCGAAA 
GGAAGTACTG CTCTTCATTA CTGCTGCTTA ACAGACAATG CCGAGTGCCT TAAACTGCTG 
CTGCGAGGCA AAGCCTCCAC AGACATAGCT AACGATGCAG GAGAAACGCC GTTGGACATA 
GCGAAAAGGC TCAAGCATGC ACAGTGCGAG GATCTGCTGA TACAAGCCAT GAATGGAAGG 
TTTAACGCTC ACGTTCATGT GGAATATGAA TGGAGGCTGC ATCACATGGA CCTAGATGAA 
AGCGATGACG ACATGGATGA AAAAATACAG CAGCCAAGCC CTATCCGCAG AGAGGAGAGG 
CCTGTCAGTT TCTTTGGAAT AAACCAGGGC CAGCCTAGTG TTGCCTGCTT AGCCAGGGAA 
GCAGCCAGTA TTGCTAAGGA CAAGCCAAGG ACTTTCCTGC CAAATATCAT TCAGAATGAA 
ACCTATGGAG CAGTCATTGG TAACAGCAAC TCCCCAGGCA ATGCTACAGG TCCTTCTACT 
ACCAATGCTC CTCCTTTGCC TCCCCGAAAC ACTATAAAAG ATTCACCGCC ACCCCCTCCG 
GTCACTAAGA CAGCCAGTGT TATTGAAGCC CTTAGCCAGC ACTCCAAGCA GAGCCCTCCA 
CCTCCAGTTC CGCTGAACAA GCCTGTCCCT CCGCCGGTCC CACCTCAGCC TGCCAACCGC 
CCCTTACACA AAAGGCCGGC TCCTGGGATT GACAAACCCC CTCCAACAGC CAAGGGGCCA 
CCTCCTGCTT TGGTGACTGC GCCACCGTCT GCAGCTGCAG AATTCCCTTC AGACACAACA 
GCCTTGCAGC CTGGATCCAA ACCCAGCAAC CGAACACAGC CACCAGCACC CATGCCACGG 
AAATCCCAGC TGCCCAAAAT GAAGCCCAAG AGGGTGAAAG CAATATACCA CTGTTCAGCT 
GACAATCCGG ATGAGCTGAC CTTCTCAGAG GGTGAAATTA TTGTGGTTGA TGGGGAGGAA 
GATAAGGAGT GGTGGATTGG ACACATAGAC GGAGAGCCAA CTCGAAGAGG TGCCTTCCCT 
GTGTCGTTTG TGCACTTTAT CGCTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818916.1
CDS	431..3340
Translation	MPDLISVSEFVCETNEDYKSPTASNFTNRLSQCRNTVVAIDEALEVDHTILNKMKKSVKAIHTSGLTHVENEEQYTQALEKFGSNCVCRDDPDLGTAFLKFSVFTKELTALFKNLVQNMNNIITFPLDSLLKGDLKGVKGELKKPFEKAWKDYETKVTKIEKEKKEHAKMHGMIRTEISGAEIAEEMEKERRVFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVETLKPSIEKLSTDLHTIKQVQDEERKQLSQLRDILKAALQVEQKEDSQIRQSVAYSLHQPQGNKEHGSERDGYLYKKSDGIRKVWQKRKCTVQNGFLTISHGTANIPPAKLNLLTCQVKTNPEEKKSFDLISHDRTYHFQAEDETECQIWMSVLQNSKEEALNNAFKGDHSAGENNIVQELTKEIISDIQRMPGNDVCCDCGASDPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLGTSELLLAKNIGNSGFNELMEACLPADDSVKPSPCSDMNARKDYITTKYIERKYARRKYHDNASKLHGLCDAVKSRDIFTLVQLHAEGVDLTEGIPLANGHEQGETALHLAVRSVDRNSLHIVDFLVQNSGNLDKQTAKGSTALHYCCLTDNAECLKLLLRGKASTDIANDAGETPLDIAKRLKHAQCEDLLIQAMNGRFNAHVHVEYEWRLHHMDLDESDDDMDEKIQQPSPIRREERPVSFFGINQGQPSVACLAREAASIAKDKPRTFLPNIIQNETYGAVIGNSNSPGNATGPSTTNAPPLPPRNTIKDSPPPPPVTKTASVIEALSQHSKQSPPPPVPLNKPVPPPVPPQPANRPLHKRPAPGIDKPPPTAKGPPPALVTAPPSAAAEFPSDTTALQPGSKPSNRTQPPAPMPRKSQLPKMKPKRVKAIYHCSADNPDELTFSEGEIIVVDGEEDKEWWIGHIDGEPTRRGAFPVSFVHFIAE

Target ORF information:

RefSeq Version	XM_012963462.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963462.2

ORF Insert Sequence:

ATGCCGGATC TGATCAGTGT GTCGGAATTT GTGTGTGAGA CCAATGAGGA TTACAAGTCC 
CCCACTGCCT CCAATTTCAC CAACAGGCTG AGCCAGTGCA GGAACACGGT CGTGGCTATA 
GATGAGGCTC TGGAGGTTGA TCACACGATT CTGAACAAAA TGAAGAAATC AGTCAAAGCC 
ATTCACACTT CTGGTCTGAC CCACGTGGAA AATGAGGAGC AGTACACTCA AGCACTGGAA 
AAATTTGGAA GCAACTGTGT TTGTCGGGAT GACCCTGATT TGGGAACAGC CTTCTTAAAA 
TTCTCCGTCT TTACCAAGGA GCTAACAGCA CTTTTCAAGA ACCTGGTACA GAACATGAAT 
AACATTATCA CATTTCCACT GGACAGCTTA CTGAAGGGAG ATCTTAAAGG GGTAAAAGGG 
GAATTGAAAA AGCCATTCGA AAAAGCCTGG AAAGACTATG AAACTAAAGT GACCAAAATT 
GAAAAGGAGA AGAAGGAGCA CGCCAAGATG CATGGTATGA TTCGCACTGA GATCAGTGGG 
GCTGAAATTG CTGAGGAAAT GGAGAAAGAA AGACGGGTTT TCCAGCTCCA AATGTGTGAG 
TACCTGCTGA AAGTGAATGA AATAAAGATT AAAAAAGGAG TAGATCTGCT GCAGAACCTG 
ATTAAGTACT TCCACGCACA GTGCAACTTT TTCCAGGATG GATTGAAAGC TGTTGAGACT 
CTGAAGCCCT CTATTGAAAA ACTTTCAACC GATCTGCACA CGATCAAGCA AGTCCAGGAT 
GAAGAGAGGA AGCAGCTTAG CCAGCTAAGG GATATCTTAA AAGCTGCCCT ACAAGTGGAG 
CAGAAGGAGG ATTCACAGAT CCGACAGAGC GTCGCTTACA GCTTGCATCA GCCACAGGGA 
AACAAGGAAC ACGGCTCTGA GAGGGACGGC TATCTCTATA AGAAGAGTGA TGGAATCAGA 
AAGGTTTGGC AGAAGAGGAA ATGCACAGTA CAAAATGGCT TTCTAACCAT ATCTCATGGC 
ACAGCAAACA TTCCGCCTGC AAAGCTGAAC CTCTTAACCT GTCAAGTGAA AACCAACCCA 
GAGGAAAAGA AGAGCTTTGA CCTTATCTCA CATGATCGGA CATATCACTT TCAAGCAGAG 
GATGAAACAG AGTGCCAAAT ATGGATGTCT GTGCTTCAGA ACAGCAAGGA AGAAGCCTTA 
AACAATGCAT TCAAGGGAGA CCACAGTGCA GGGGAGAACA ACATTGTTCA GGAACTCACT 
AAGGAAATAA TATCTGATAT CCAGAGAATG CCAGGGAACG ACGTGTGCTG TGACTGTGGT 
GCATCAGATC CAACTTGGCT TTCCACAAAC CTGGGGATCC TTACATGCAT TGAGTGCTCT 
GGGATACACA GAGAGCTGGG CGTGCATTAC TCCAGAATAC AGTCTCTTAC ACTGGATGTC 
TTGGGAACTT CTGAGCTTTT GCTTGCCAAA AACATTGGGA ACTCAGGTTT TAATGAACTA 
ATGGAAGCCT GTCTGCCTGC TGATGATTCC GTGAAGCCTT CACCTTGCAG TGACATGAAT 
GCACGGAAAG ACTATATCAC CACCAAGTAC ATAGAAAGAA AGTACGCAAG GAGAAAATAC 
CATGACAATG CCTCTAAACT GCATGGCTTG TGTGACGCCG TCAAATCCAG AGACATCTTT 
ACACTGGTAC AGTTACATGC TGAAGGGGTG GATCTCACCG AGGGCATACC ACTTGCCAAT 
GGACACGAAC AAGGTGAGAC TGCACTCCAT CTCGCTGTCA GATCAGTTGA CCGCAATTCC 
TTGCACATAG TCGACTTTCT TGTACAGAAT AGTGGCAACT TGGACAAACA GACAGCGAAA 
GGAAGTACTG CTCTTCATTA CTGCTGCTTA ACAGACAATG CCGAGTGCCT TAAACTGCTG 
CTGCGAGGCA AAGCCTCCAC AGACATAGCT AACGATGCAG GAGAAACGCC GTTGGACATA 
GCGAAAAGGC TCAAGCATGC ACAGTGCGAG GATCTGCTGA TACAAGCCAT GAATGGAAGG 
TTTAACGCTC ACGTTCATGT GGAATATGAA TGGAGGCTGC ATCACATGGA CCTAGATGAA 
AGCGATGACG ACATGGATGA AAAAATACAG CAGCCAAGCC CTATCCGCAG AGAGGAGAGG 
CCTGTCAGTT TCTTTGGAAT AAACCAGGGC CAGCCTAGTG TTGCCTGCTT AGCCAGGGAA 
GCAGCCAGTA TTGCTAAGGA CAAGCCAAGG ACTTTCCTGC CAAATATCAT TCAGAATGAA 
ACCTATGGAG CAGTCATTGG TAACAGCAAC TCCCCAGGCA ATGCTACAGG TCCTTCTACT 
ACCAATGCTC CTCCTTTGCC TCCCCGAAAC ACTATAAAAG ATTCACCGCC ACCCCCTCCG 
GTCACTAAGA CAGCCAGTGT TATTGAAGCC CTTAGCCAGC ACTCCAAGCA GAGCCCTCCA 
CCTCCAGTTC CGCTGAACAA GCCTGTCCCT CCGCCGGTCC CACCTCAGCC TGCCAACCGC 
CCCTTACACA AAAGGCCGGC TCCTGGGATT GACAAACCCC CTCCAACAGC CAAGGGGCCA 
CCTCCTGCTT TGGTGACTGC GCCACCGTCT GCAGCTGCAG AATTCCCTTC AGACACAACA 
GCCTTGCAGC CTGGATCCAA ACCCAGCAAC CGAACACAGC CACCAGCACC CATGCCACGG 
AAATCCCAGC TGCCCAAAAT GAAGCCCAAG AGGGTGAAAG CAATATACCA CTGTTCAGCT 
GACAATCCGG ATGAGCTGAC CTTCTCAGAG GGTGAAATTA TTGTGGTTGA TGGGGAGGAA 
GATAAGGAGT GGTGGATTGG ACACATAGAC GGAGAGCCAA CTCGAAGAGG TGCCTTCCCT 
GTGTCGTTTG TGCACTTTAT CGCTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2 ( XM_012963462.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818916.2 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa59216
Clone ID Related Accession (Same CDS sequence)	XM_012963462.3
Accession Version	XM_012963462.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963462.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGATC TGATCAGTGT GTCGGAATTT GTGTGTGAGA CCAATGAGGA TTACAAGTCC 
CCCACTGCCT CCAATTTCAC CAACAGGCTG AGCCAGTGCA GGAACACGGT CGTGGCTATA 
GATGAGGCTC TGGAGGTTGA TCACACGATT CTGAACAAAA TGAAGAAATC AGTCAAAGCC 
ATTCACACTT CTGGTCTGAC CCACGTGGAA AATGAGGAGC AGTACACTCA AGCACTGGAA 
AAATTTGGAA GCAACTGTGT TTGTCGGGAT GACCCTGATT TGGGAACAGC CTTCTTAAAA 
TTCTCCGTCT TTACCAAGGA GCTAACAGCA CTTTTCAAGA ACCTGGTACA GAACATGAAT 
AACATTATCA CATTTCCACT GGACAGCTTA CTGAAGGGAG ATCTTAAAGG GGTAAAAGGG 
GAATTGAAAA AGCCATTCGA AAAAGCCTGG AAAGACTATG AAACTAAAGT GACCAAAATT 
GAAAAGGAGA AGAAGGAGCA CGCCAAGATG CATGGTATGA TTCGCACTGA GATCAGTGGG 
GCTGAAATTG CTGAGGAAAT GGAGAAAGAA AGACGGGTTT TCCAGCTCCA AATGTGTGAG 
TACCTGCTGA AAGTGAATGA AATAAAGATT AAAAAAGGAG TAGATCTGCT GCAGAACCTG 
ATTAAGTACT TCCACGCACA GTGCAACTTT TTCCAGGATG GATTGAAAGC TGTTGAGACT 
CTGAAGCCCT CTATTGAAAA ACTTTCAACC GATCTGCACA CGATCAAGCA AGTCCAGGAT 
GAAGAGAGGA AGCAGCTTAG CCAGCTAAGG GATATCTTAA AAGCTGCCCT ACAAGTGGAG 
CAGAAGGAGG ATTCACAGAT CCGACAGAGC GTCGCTTACA GCTTGCATCA GCCACAGGGA 
AACAAGGAAC ACGGCTCTGA GAGGGACGGC TATCTCTATA AGAAGAGTGA TGGAATCAGA 
AAGGTTTGGC AGAAGAGGAA ATGCACAGTA CAAAATGGCT TTCTAACCAT ATCTCATGGC 
ACAGCAAACA TTCCGCCTGC AAAGCTGAAC CTCTTAACCT GTCAAGTGAA AACCAACCCA 
GAGGAAAAGA AGAGCTTTGA CCTTATCTCA CATGATCGGA CATATCACTT TCAAGCAGAG 
GATGAAACAG AGTGCCAAAT ATGGATGTCT GTGCTTCAGA ACAGCAAGGA AGAAGCCTTA 
AACAATGCAT TCAAGGGAGA CCACAGTGCA GGGGAGAACA ACATTGTTCA GGAACTCACT 
AAGGAAATAA TATCTGATAT CCAGAGAATG CCAGGGAACG ACGTGTGCTG TGACTGTGGT 
GCATCAGATC CAACTTGGCT TTCCACAAAC CTGGGGATCC TTACATGCAT TGAGTGCTCT 
GGGATACACA GAGAGCTGGG CGTGCATTAC TCCAGAATAC AGTCTCTTAC ACTGGATGTC 
TTGGGAACTT CTGAGCTTTT GCTTGCCAAA AACATTGGGA ACTCAGGTTT TAATGAACTA 
ATGGAAGCCT GTCTGCCTGC TGATGATTCC GTGAAGCCTT CACCTTGCAG TGACATGAAT 
GCACGGAAAG ACTATATCAC CACCAAGTAC ATAGAAAGAA AGTACGCAAG GAGAAAATAC 
CATGACAATG CCTCTAAACT GCATGGCTTG TGTGACGCCG TCAAATCCAG AGACATCTTT 
ACACTGGTAC AGTTACATGC TGAAGGGGTG GATCTCACCG AGGGCATACC ACTTGCCAAT 
GGACACGAAC AAGGTGAGAC TGCACTCCAT CTCGCTGTCA GATCAGTTGA CCGCAATTCC 
TTGCACATAG TCGACTTTCT TGTACAGAAT AGTGGCAACT TGGACAAACA GACAGCGAAA 
GGAAGTACTG CTCTTCATTA CTGCTGCTTA ACAGACAATG CCGAGTGCCT TAAACTGCTG 
CTGCGAGGCA AAGCCTCCAC AGACATAGCT AACGATGCAG GAGAAACGCC GTTGGACATA 
GCGAAAAGGC TCAAGCATGC ACAGTGCGAG GATCTGCTGA TACAAGCCAT GAATGGAAGG 
TTTAACGCTC ACGTTCATGT GGAATATGAA TGGAGGCTGC ATCACATGGA CCTAGATGAA 
AGCGATGACG ACATGGATGA AAAAATACAG CAGCCAAGCC CTATCCGCAG AGAGGAGAGG 
CCTGTCAGTT TCTTTGGAAT AAACCAGGGC CAGCCTAGTG TTGCCTGCTT AGCCAGGGAA 
GCAGCCAGTA TTGCTAAGGA CAAGCCAAGG ACTTTCCTGC CAAATATCAT TCAGAATGAA 
ACCTATGGAG CAGTCATTGG TAACAGCAAC TCCCCAGGCA ATGCTACAGG TCCTTCTACT 
ACCAATGCTC CTCCTTTGCC TCCCCGAAAC ACTATAAAAG ATTCACCGCC ACCCCCTCCG 
GTCACTAAGA CAGCCAGTGT TATTGAAGCC CTTAGCCAGC ACTCCAAGCA GAGCCCTCCA 
CCTCCAGTTC CGCTGAACAA GCCTGTCCCT CCGCCGGTCC CACCTCAGCC TGCCAACCGC 
CCCTTACACA AAAGGCCGGC TCCTGGGATT GACAAACCCC CTCCAACAGC CAAGGGGCCA 
CCTCCTGCTT TGGTGACTGC GCCACCGTCT GCAGCTGCAG AATTCCCTTC AGACACAACA 
GCCTTGCAGC CTGGATCCAA ACCCAGCAAC CGAACACAGC CACCAGCACC CATGCCACGG 
AAATCCCAGC TGCCCAAAAT GAAGCCCAAG AGGGTGAAAG CAATATACCA CTGTTCAGCT 
GACAATCCGG ATGAGCTGAC CTTCTCAGAG GGTGAAATTA TTGTGGTTGA TGGGGAGGAA 
GATAAGGAGT GGTGGATTGG ACACATAGAC GGAGAGCCAA ACCGAAGAGG TGCCTTCCCT 
GTGTCGTTTG TGCACTTTAT CGCTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818916.2
CDS	416..3325
Translation	MPDLISVSEFVCETNEDYKSPTASNFTNRLSQCRNTVVAIDEALEVDHTILNKMKKSVKAIHTSGLTHVENEEQYTQALEKFGSNCVCRDDPDLGTAFLKFSVFTKELTALFKNLVQNMNNIITFPLDSLLKGDLKGVKGELKKPFEKAWKDYETKVTKIEKEKKEHAKMHGMIRTEISGAEIAEEMEKERRVFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVETLKPSIEKLSTDLHTIKQVQDEERKQLSQLRDILKAALQVEQKEDSQIRQSVAYSLHQPQGNKEHGSERDGYLYKKSDGIRKVWQKRKCTVQNGFLTISHGTANIPPAKLNLLTCQVKTNPEEKKSFDLISHDRTYHFQAEDETECQIWMSVLQNSKEEALNNAFKGDHSAGENNIVQELTKEIISDIQRMPGNDVCCDCGASDPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLGTSELLLAKNIGNSGFNELMEACLPADDSVKPSPCSDMNARKDYITTKYIERKYARRKYHDNASKLHGLCDAVKSRDIFTLVQLHAEGVDLTEGIPLANGHEQGETALHLAVRSVDRNSLHIVDFLVQNSGNLDKQTAKGSTALHYCCLTDNAECLKLLLRGKASTDIANDAGETPLDIAKRLKHAQCEDLLIQAMNGRFNAHVHVEYEWRLHHMDLDESDDDMDEKIQQPSPIRREERPVSFFGINQGQPSVACLAREAASIAKDKPRTFLPNIIQNETYGAVIGNSNSPGNATGPSTTNAPPLPPRNTIKDSPPPPPVTKTASVIEALSQHSKQSPPPPVPLNKPVPPPVPPQPANRPLHKRPAPGIDKPPPTAKGPPPALVTAPPSAAAEFPSDTTALQPGSKPSNRTQPPAPMPRKSQLPKMKPKRVKAIYHCSADNPDELTFSEGEIIVVDGEEDKEWWIGHIDGEPNRRGAFPVSFVHFIAE

Target ORF information:

RefSeq Version	XM_012963462.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963462.3

ORF Insert Sequence:

ATGCCGGATC TGATCAGTGT GTCGGAATTT GTGTGTGAGA CCAATGAGGA TTACAAGTCC 
CCCACTGCCT CCAATTTCAC CAACAGGCTG AGCCAGTGCA GGAACACGGT CGTGGCTATA 
GATGAGGCTC TGGAGGTTGA TCACACGATT CTGAACAAAA TGAAGAAATC AGTCAAAGCC 
ATTCACACTT CTGGTCTGAC CCACGTGGAA AATGAGGAGC AGTACACTCA AGCACTGGAA 
AAATTTGGAA GCAACTGTGT TTGTCGGGAT GACCCTGATT TGGGAACAGC CTTCTTAAAA 
TTCTCCGTCT TTACCAAGGA GCTAACAGCA CTTTTCAAGA ACCTGGTACA GAACATGAAT 
AACATTATCA CATTTCCACT GGACAGCTTA CTGAAGGGAG ATCTTAAAGG GGTAAAAGGG 
GAATTGAAAA AGCCATTCGA AAAAGCCTGG AAAGACTATG AAACTAAAGT GACCAAAATT 
GAAAAGGAGA AGAAGGAGCA CGCCAAGATG CATGGTATGA TTCGCACTGA GATCAGTGGG 
GCTGAAATTG CTGAGGAAAT GGAGAAAGAA AGACGGGTTT TCCAGCTCCA AATGTGTGAG 
TACCTGCTGA AAGTGAATGA AATAAAGATT AAAAAAGGAG TAGATCTGCT GCAGAACCTG 
ATTAAGTACT TCCACGCACA GTGCAACTTT TTCCAGGATG GATTGAAAGC TGTTGAGACT 
CTGAAGCCCT CTATTGAAAA ACTTTCAACC GATCTGCACA CGATCAAGCA AGTCCAGGAT 
GAAGAGAGGA AGCAGCTTAG CCAGCTAAGG GATATCTTAA AAGCTGCCCT ACAAGTGGAG 
CAGAAGGAGG ATTCACAGAT CCGACAGAGC GTCGCTTACA GCTTGCATCA GCCACAGGGA 
AACAAGGAAC ACGGCTCTGA GAGGGACGGC TATCTCTATA AGAAGAGTGA TGGAATCAGA 
AAGGTTTGGC AGAAGAGGAA ATGCACAGTA CAAAATGGCT TTCTAACCAT ATCTCATGGC 
ACAGCAAACA TTCCGCCTGC AAAGCTGAAC CTCTTAACCT GTCAAGTGAA AACCAACCCA 
GAGGAAAAGA AGAGCTTTGA CCTTATCTCA CATGATCGGA CATATCACTT TCAAGCAGAG 
GATGAAACAG AGTGCCAAAT ATGGATGTCT GTGCTTCAGA ACAGCAAGGA AGAAGCCTTA 
AACAATGCAT TCAAGGGAGA CCACAGTGCA GGGGAGAACA ACATTGTTCA GGAACTCACT 
AAGGAAATAA TATCTGATAT CCAGAGAATG CCAGGGAACG ACGTGTGCTG TGACTGTGGT 
GCATCAGATC CAACTTGGCT TTCCACAAAC CTGGGGATCC TTACATGCAT TGAGTGCTCT 
GGGATACACA GAGAGCTGGG CGTGCATTAC TCCAGAATAC AGTCTCTTAC ACTGGATGTC 
TTGGGAACTT CTGAGCTTTT GCTTGCCAAA AACATTGGGA ACTCAGGTTT TAATGAACTA 
ATGGAAGCCT GTCTGCCTGC TGATGATTCC GTGAAGCCTT CACCTTGCAG TGACATGAAT 
GCACGGAAAG ACTATATCAC CACCAAGTAC ATAGAAAGAA AGTACGCAAG GAGAAAATAC 
CATGACAATG CCTCTAAACT GCATGGCTTG TGTGACGCCG TCAAATCCAG AGACATCTTT 
ACACTGGTAC AGTTACATGC TGAAGGGGTG GATCTCACCG AGGGCATACC ACTTGCCAAT 
GGACACGAAC AAGGTGAGAC TGCACTCCAT CTCGCTGTCA GATCAGTTGA CCGCAATTCC 
TTGCACATAG TCGACTTTCT TGTACAGAAT AGTGGCAACT TGGACAAACA GACAGCGAAA 
GGAAGTACTG CTCTTCATTA CTGCTGCTTA ACAGACAATG CCGAGTGCCT TAAACTGCTG 
CTGCGAGGCA AAGCCTCCAC AGACATAGCT AACGATGCAG GAGAAACGCC GTTGGACATA 
GCGAAAAGGC TCAAGCATGC ACAGTGCGAG GATCTGCTGA TACAAGCCAT GAATGGAAGG 
TTTAACGCTC ACGTTCATGT GGAATATGAA TGGAGGCTGC ATCACATGGA CCTAGATGAA 
AGCGATGACG ACATGGATGA AAAAATACAG CAGCCAAGCC CTATCCGCAG AGAGGAGAGG 
CCTGTCAGTT TCTTTGGAAT AAACCAGGGC CAGCCTAGTG TTGCCTGCTT AGCCAGGGAA 
GCAGCCAGTA TTGCTAAGGA CAAGCCAAGG ACTTTCCTGC CAAATATCAT TCAGAATGAA 
ACCTATGGAG CAGTCATTGG TAACAGCAAC TCCCCAGGCA ATGCTACAGG TCCTTCTACT 
ACCAATGCTC CTCCTTTGCC TCCCCGAAAC ACTATAAAAG ATTCACCGCC ACCCCCTCCG 
GTCACTAAGA CAGCCAGTGT TATTGAAGCC CTTAGCCAGC ACTCCAAGCA GAGCCCTCCA 
CCTCCAGTTC CGCTGAACAA GCCTGTCCCT CCGCCGGTCC CACCTCAGCC TGCCAACCGC 
CCCTTACACA AAAGGCCGGC TCCTGGGATT GACAAACCCC CTCCAACAGC CAAGGGGCCA 
CCTCCTGCTT TGGTGACTGC GCCACCGTCT GCAGCTGCAG AATTCCCTTC AGACACAACA 
GCCTTGCAGC CTGGATCCAA ACCCAGCAAC CGAACACAGC CACCAGCACC CATGCCACGG 
AAATCCCAGC TGCCCAAAAT GAAGCCCAAG AGGGTGAAAG CAATATACCA CTGTTCAGCT 
GACAATCCGG ATGAGCTGAC CTTCTCAGAG GGTGAAATTA TTGTGGTTGA TGGGGAGGAA 
GATAAGGAGT GGTGGATTGG ACACATAGAC GGAGAGCCAA ACCGAAGAGG TGCCTTCCCT 
GTGTCGTTTG TGCACTTTAT CGCTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2 ( XM_002935032.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935078.3 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa59214
Clone ID Related Accession (Same CDS sequence)	XM_002935032.5
Accession Version	XM_002935032.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935032.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGATC TGATCAGTGT GTCGGAATTT GTGTGTGAGA CCAATGAGGA TTACAAGTCC 
CCCACTGCCT CCAATTTCAC CAACAGGCTG AGCCAGTGCA GGAACACGGT CGTGGCTATA 
GATGAGGCTC TGGAGGTTGA TCACACGATT CTGAACAAAA TGAAGAAATC AGTCAAAGCC 
ATTCACACTT CTGGTCTGAC CCACGTGGAA AATGAGGAGC AGTACACTCA AGCACTGGAA 
AAATTTGGAA GCAACTGTGT TTGTCGGGAT GACCCTGATT TGGGAACAGC CTTCTTAAAA 
TTCTCCGTCT TTACCAAGGA GCTAACAGCA CTTTTCAAGA ACCTGGTACA GAACATGAAT 
AACATTATCA CATTTCCACT GGACAGCTTA CTGAAGGGAG ATCTTAAAGG GGTAAAAGGG 
GAATTGAAAA AGCCATTCGA AAAAGCCTGG AAAGACTATG AAACTAAAGT GACCAAAATT 
GAAAAGGAGA AGAAGGAGCA CGCCAAGATG CATGGTATGA TTCGCACTGA GATCAGTGGG 
GCTGAAATTG CTGAGGAAAT GGAGAAAGAA AGACGGGTTT TCCAGCTCCA AATGTGTGAG 
TACCTGCTGA AAGTGAATGA AATAAAGATT AAAAAAGGAG TAGATCTGCT GCAGAACCTG 
ATTAAGTACT TCCACGCACA GTGCAACTTT TTCCAGGATG GATTGAAAGC TGTTGAGACT 
CTGAAGCCCT CTATTGAAAA ACTTTCAACC GATCTGCACA CGATCAAGCA AGTCCAGGAT 
GAAGAGAGGA AGCAGCTTAG CCAGCTAAGG GATATCTTAA AAGCTGCCCT ACAAGTGGAG 
CAGAAGGAGG ATTCACAGAT CCGACAGAGC GTCGCTTACA GCTTGCATCA GCCACAGGGA 
AACAAGGAAC ACGGCTCTGA GAGGGACGGC TATCTCTATA AGAAGAGTGA TGGAATCAGA 
AAGGTTTGGC AGAAGAGGAA ATGCACAGTA CAAAATGGCT TTCTAACCAT ATCTCATGGC 
ACAGCAAACA TTCCGCCTGC AAAGCTGAAC CTCTTAACCT GTCAAGTGAA AACCAACCCA 
GAGGAAAAGA AGAGCTTTGA CCTTATCTCA CATGATCGGA CATATCACTT TCAAGCAGAG 
GATGAAACAG AGTGCCAAAT ATGGATGTCT GTGCTTCAGA ACAGCAAGGA AGAAGCCTTA 
AACAATGCAT TCAAGGGAGA CCACAGTGCA GGGGAGAACA ACATTGTTCA GGAACTCACT 
AAGGAAATAA TATCTGATAT CCAGAGAATG CCAGGGAACG ACGTGTGCTG TGACTGTGGT 
GCATCAGATC CAACTTGGCT TTCCACAAAC CTGGGGATCC TTACATGCAT TGAGTGCTCT 
GGGATACACA GAGAGCTGGG CGTGCATTAC TCCAGAATAC AGTCTCTTAC ACTGGATGTC 
TTGGGAACTT CTGAGCTTTT GCTTGCCAAA AACATTGGGA ACTCAGGTTT TAATGAACTA 
ATGGAAGCCT GTCTGCCTGC TGATGATTCC GTGAAGCCTT CACCTTGCAG TGACATGAAT 
GCACGGAAAG ACTATATCAC CACCAAGTAC ATAGAAAGAA AGTACGCAAG GAGAAAATAC 
CATGACAATG CCTCTAAACT GCATGGCTTG TGTGACGCCG TCAAATCCAG AGACATCTTT 
ACACTGGTAC AGTTACATGC TGAAGGGGTG GATCTCACCG AGGGCATACC ACTTGCCAAT 
GGACACGAAC AAGGTGAGAC TGCACTCCAT CTCGCTGTCA GATCAGTTGA CCGCAATTCC 
TTGCACATAG TCGACTTTCT TGTACAGAAT AGTGGCAACT TGGACAAACA GACAGCGAAA 
GGAAGTACTG CTCTTCATTA CTGCTGCTTA ACAGACAATG CCGAGTGCCT TAAACTGCTG 
CTGCGAGGCA AAGCCTCCAC AGACATAGCT AACGATGCAG GAGAAACGCC GTTGGACATA 
GCGAAAAGGC TCAAGCATGC ACAGTGCGAG GATCTGCTGA TACAAGCCAT GAATGGAAGG 
TTTAACGCTC ACGTTCATGT GGAATATGAA TGGAGGCTGC ATCACATGGA CCTAGATGAA 
AGCGATGACG ACATGGATGA AAAAATACAG CAGCCAAGCC CTATCCGCAG AGAGGAGAGG 
CCTGTCAGTT TCTTTGGAAT AAACCAGGGC CAGCCTAGTG TTGCCTGCTT AGCCAGGGAA 
GCAGCCAGTA TTGCTAAGGA CAAGCCAAGG ACTTTCCTGC CAAATATCAT TCAGAATGAA 
ACCTATGGAG CAGTCATTGG TAACAGCAAC TCCCCAGGCA ATGCTACAGG TCCTTCTACT 
ACCAATGCTC CTCCTTTGCC TCCCCGAAAC ACTATAAAAG TTCATTCAAC TATATTTGGC 
AGTAGCATAC ACCCCACACC TTCCAACACC CCAGCCTGGA AAGCTAGCTC AGTAACACCG 
GAAAGTGGCA GCAGACAGAG GTCCTCATCT GATCCGCCAT CTATACAGCC CCCTGTGCCA 
CCTGTCCGCG TAACATCCAC TGCCACAAAT TCACCGCCAC CCCCTCCGGT CACTAAGACA 
GCCAGTGTTA TTGAAGCCCT TAGCCAGCAC TCCAAGCAGA GCCCTCCACC TCCAGTTCCG 
CTGAACAAGC CTGTCCCTCC GCCGGTCCCA CCTCAGCCTG CCAACCGCCC CTTACACAAA 
AGGCCGGCTC CTGGGATTGA CAAACCCCCT CCAACAGCCA AGGGGCCACC TCCTGCTTTG 
GTGACTGCGC CACCGTCTGC AGCTGCAGAA TTCCCTTCAG ACACAACAGC CTTGCAGCCT 
GGATCCAAAC CCAGCAACCG AACACAGCCA CCAGCACCCA TGCCACGGAA ATCCCAGCTG 
CCCAAAATGA AGCCCAAGAG GGTGAAAGCA ATATACCACT GTTCAGCTGA CAATCCGGAT 
GAGCTGACCT TCTCAGAGGG TGAAATTATT GTGGTTGATG GGGAGGAAGA TAAGGAGTGG 
TGGATTGGAC ACATAGACGG AGAGCCAAAC CGAAGAGGTG CCTTCCCTGT GTCGTTTGTG 
CACTTTATCG CTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935078.3
CDS	416..3493
Translation	MPDLISVSEFVCETNEDYKSPTASNFTNRLSQCRNTVVAIDEALEVDHTILNKMKKSVKAIHTSGLTHVENEEQYTQALEKFGSNCVCRDDPDLGTAFLKFSVFTKELTALFKNLVQNMNNIITFPLDSLLKGDLKGVKGELKKPFEKAWKDYETKVTKIEKEKKEHAKMHGMIRTEISGAEIAEEMEKERRVFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVETLKPSIEKLSTDLHTIKQVQDEERKQLSQLRDILKAALQVEQKEDSQIRQSVAYSLHQPQGNKEHGSERDGYLYKKSDGIRKVWQKRKCTVQNGFLTISHGTANIPPAKLNLLTCQVKTNPEEKKSFDLISHDRTYHFQAEDETECQIWMSVLQNSKEEALNNAFKGDHSAGENNIVQELTKEIISDIQRMPGNDVCCDCGASDPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLGTSELLLAKNIGNSGFNELMEACLPADDSVKPSPCSDMNARKDYITTKYIERKYARRKYHDNASKLHGLCDAVKSRDIFTLVQLHAEGVDLTEGIPLANGHEQGETALHLAVRSVDRNSLHIVDFLVQNSGNLDKQTAKGSTALHYCCLTDNAECLKLLLRGKASTDIANDAGETPLDIAKRLKHAQCEDLLIQAMNGRFNAHVHVEYEWRLHHMDLDESDDDMDEKIQQPSPIRREERPVSFFGINQGQPSVACLAREAASIAKDKPRTFLPNIIQNETYGAVIGNSNSPGNATGPSTTNAPPLPPRNTIKVHSTIFGSSIHPTPSNTPAWKASSVTPESGSRQRSSSDPPSIQPPVPPVRVTSTATNSPPPPPVTKTASVIEALSQHSKQSPPPPVPLNKPVPPPVPPQPANRPLHKRPAPGIDKPPPTAKGPPPALVTAPPSAAAEFPSDTTALQPGSKPSNRTQPPAPMPRKSQLPKMKPKRVKAIYHCSADNPDELTFSEGEIIVVDGEEDKEWWIGHIDGEPNRRGAFPVSFVHFIAE

Target ORF information:

RefSeq Version	XM_002935032.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935032.5

ORF Insert Sequence:

ATGCCGGATC TGATCAGTGT GTCGGAATTT GTGTGTGAGA CCAATGAGGA TTACAAGTCC 
CCCACTGCCT CCAATTTCAC CAACAGGCTG AGCCAGTGCA GGAACACGGT CGTGGCTATA 
GATGAGGCTC TGGAGGTTGA TCACACGATT CTGAACAAAA TGAAGAAATC AGTCAAAGCC 
ATTCACACTT CTGGTCTGAC CCACGTGGAA AATGAGGAGC AGTACACTCA AGCACTGGAA 
AAATTTGGAA GCAACTGTGT TTGTCGGGAT GACCCTGATT TGGGAACAGC CTTCTTAAAA 
TTCTCCGTCT TTACCAAGGA GCTAACAGCA CTTTTCAAGA ACCTGGTACA GAACATGAAT 
AACATTATCA CATTTCCACT GGACAGCTTA CTGAAGGGAG ATCTTAAAGG GGTAAAAGGG 
GAATTGAAAA AGCCATTCGA AAAAGCCTGG AAAGACTATG AAACTAAAGT GACCAAAATT 
GAAAAGGAGA AGAAGGAGCA CGCCAAGATG CATGGTATGA TTCGCACTGA GATCAGTGGG 
GCTGAAATTG CTGAGGAAAT GGAGAAAGAA AGACGGGTTT TCCAGCTCCA AATGTGTGAG 
TACCTGCTGA AAGTGAATGA AATAAAGATT AAAAAAGGAG TAGATCTGCT GCAGAACCTG 
ATTAAGTACT TCCACGCACA GTGCAACTTT TTCCAGGATG GATTGAAAGC TGTTGAGACT 
CTGAAGCCCT CTATTGAAAA ACTTTCAACC GATCTGCACA CGATCAAGCA AGTCCAGGAT 
GAAGAGAGGA AGCAGCTTAG CCAGCTAAGG GATATCTTAA AAGCTGCCCT ACAAGTGGAG 
CAGAAGGAGG ATTCACAGAT CCGACAGAGC GTCGCTTACA GCTTGCATCA GCCACAGGGA 
AACAAGGAAC ACGGCTCTGA GAGGGACGGC TATCTCTATA AGAAGAGTGA TGGAATCAGA 
AAGGTTTGGC AGAAGAGGAA ATGCACAGTA CAAAATGGCT TTCTAACCAT ATCTCATGGC 
ACAGCAAACA TTCCGCCTGC AAAGCTGAAC CTCTTAACCT GTCAAGTGAA AACCAACCCA 
GAGGAAAAGA AGAGCTTTGA CCTTATCTCA CATGATCGGA CATATCACTT TCAAGCAGAG 
GATGAAACAG AGTGCCAAAT ATGGATGTCT GTGCTTCAGA ACAGCAAGGA AGAAGCCTTA 
AACAATGCAT TCAAGGGAGA CCACAGTGCA GGGGAGAACA ACATTGTTCA GGAACTCACT 
AAGGAAATAA TATCTGATAT CCAGAGAATG CCAGGGAACG ACGTGTGCTG TGACTGTGGT 
GCATCAGATC CAACTTGGCT TTCCACAAAC CTGGGGATCC TTACATGCAT TGAGTGCTCT 
GGGATACACA GAGAGCTGGG CGTGCATTAC TCCAGAATAC AGTCTCTTAC ACTGGATGTC 
TTGGGAACTT CTGAGCTTTT GCTTGCCAAA AACATTGGGA ACTCAGGTTT TAATGAACTA 
ATGGAAGCCT GTCTGCCTGC TGATGATTCC GTGAAGCCTT CACCTTGCAG TGACATGAAT 
GCACGGAAAG ACTATATCAC CACCAAGTAC ATAGAAAGAA AGTACGCAAG GAGAAAATAC 
CATGACAATG CCTCTAAACT GCATGGCTTG TGTGACGCCG TCAAATCCAG AGACATCTTT 
ACACTGGTAC AGTTACATGC TGAAGGGGTG GATCTCACCG AGGGCATACC ACTTGCCAAT 
GGACACGAAC AAGGTGAGAC TGCACTCCAT CTCGCTGTCA GATCAGTTGA CCGCAATTCC 
TTGCACATAG TCGACTTTCT TGTACAGAAT AGTGGCAACT TGGACAAACA GACAGCGAAA 
GGAAGTACTG CTCTTCATTA CTGCTGCTTA ACAGACAATG CCGAGTGCCT TAAACTGCTG 
CTGCGAGGCA AAGCCTCCAC AGACATAGCT AACGATGCAG GAGAAACGCC GTTGGACATA 
GCGAAAAGGC TCAAGCATGC ACAGTGCGAG GATCTGCTGA TACAAGCCAT GAATGGAAGG 
TTTAACGCTC ACGTTCATGT GGAATATGAA TGGAGGCTGC ATCACATGGA CCTAGATGAA 
AGCGATGACG ACATGGATGA AAAAATACAG CAGCCAAGCC CTATCCGCAG AGAGGAGAGG 
CCTGTCAGTT TCTTTGGAAT AAACCAGGGC CAGCCTAGTG TTGCCTGCTT AGCCAGGGAA 
GCAGCCAGTA TTGCTAAGGA CAAGCCAAGG ACTTTCCTGC CAAATATCAT TCAGAATGAA 
ACCTATGGAG CAGTCATTGG TAACAGCAAC TCCCCAGGCA ATGCTACAGG TCCTTCTACT 
ACCAATGCTC CTCCTTTGCC TCCCCGAAAC ACTATAAAAG TTCATTCAAC TATATTTGGC 
AGTAGCATAC ACCCCACACC TTCCAACACC CCAGCCTGGA AAGCTAGCTC AGTAACACCG 
GAAAGTGGCA GCAGACAGAG GTCCTCATCT GATCCGCCAT CTATACAGCC CCCTGTGCCA 
CCTGTCCGCG TAACATCCAC TGCCACAAAT TCACCGCCAC CCCCTCCGGT CACTAAGACA 
GCCAGTGTTA TTGAAGCCCT TAGCCAGCAC TCCAAGCAGA GCCCTCCACC TCCAGTTCCG 
CTGAACAAGC CTGTCCCTCC GCCGGTCCCA CCTCAGCCTG CCAACCGCCC CTTACACAAA 
AGGCCGGCTC CTGGGATTGA CAAACCCCCT CCAACAGCCA AGGGGCCACC TCCTGCTTTG 
GTGACTGCGC CACCGTCTGC AGCTGCAGAA TTCCCTTCAG ACACAACAGC CTTGCAGCCT 
GGATCCAAAC CCAGCAACCG AACACAGCCA CCAGCACCCA TGCCACGGAA ATCCCAGCTG 
CCCAAAATGA AGCCCAAGAG GGTGAAAGCA ATATACCACT GTTCAGCTGA CAATCCGGAT 
GAGCTGACCT TCTCAGAGGG TGAAATTATT GTGGTTGATG GGGAGGAAGA TAAGGAGTGG 
TGGATTGGAC ACATAGACGG AGAGCCAAAC CGAAGAGGTG CCTTCCCTGT GTCGTTTGTG 
CACTTTATCG CTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2 ( XM_004914478.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914535.2 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa59215
Clone ID Related Accession (Same CDS sequence)	XM_004914478.4
Accession Version	XM_004914478.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914478.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGATC TGATCAGTGT GTCGGAATTT GTGTGTGAGA CCAATGAGGA TTACAAGTCC 
CCCACTGCCT CCAATTTCAC CAACAGGCTG AGCCAGTGCA GGAACACGGT CGTGGCTATA 
GATGAGGCTC TGGAGGTTGA TCACACGATT CTGAACAAAA TGAAGAAATC AGTCAAAGCC 
ATTCACACTT CTGGTCTGAC CCACGTGGAA AATGAGGAGC AGTACACTCA AGCACTGGAA 
AAATTTGGAA GCAACTGTGT TTGTCGGGAT GACCCTGATT TGGGAACAGC CTTCTTAAAA 
TTCTCCGTCT TTACCAAGGA GCTAACAGCA CTTTTCAAGA ACCTGGTACA GAACATGAAT 
AACATTATCA CATTTCCACT GGACAGCTTA CTGAAGGGAG ATCTTAAAGG GGTAAAAGGG 
GAATTGAAAA AGCCATTCGA AAAAGCCTGG AAAGACTATG AAACTAAAGT GACCAAAATT 
GAAAAGGAGA AGAAGGAGCA CGCCAAGATG CATGGTATGA TTCGCACTGA GATCAGTGGG 
GCTGAAATTG CTGAGGAAAT GGAGAAAGAA AGACGGGTTT TCCAGCTCCA AATGTGTGAG 
TACCTGCTGA AAGTGAATGA AATAAAGATT AAAAAAGGAG TAGATCTGCT GCAGAACCTG 
ATTAAGTACT TCCACGCACA GTGCAACTTT TTCCAGGATG GATTGAAAGC TGTTGAGACT 
CTGAAGCCCT CTATTGAAAA ACTTTCAACC GATCTGCACA CGATCAAGCA AGTCCAGGAT 
GAAGAGAGGA AGCAGCTTAG CCAGCTAAGG GATATCTTAA AAGCTGCCCT ACAAGTGGAG 
CAGAAGGAGG ATTCACAGAT CCGACAGAGC GTCGCTTACA GCTTGCATCA GCCACAGGGA 
AACAAGGAAC ACGGCTCTGA GAGGGACGGC TATCTCTATA AGAAGAGTGA TGGAATCAGA 
AAGGTTTGGC AGAAGAGGAA ATGCACAGTA CAAAATGGCT TTCTAACCAT ATCTCATGGC 
ACAGCAAACA TTCCGCCTGC AAAGCTGAAC CTCTTAACCT GTCAAGTGAA AACCAACCCA 
GAGGAAAAGA AGAGCTTTGA CCTTATCTCA CATGATCGGA CATATCACTT TCAAGCAGAG 
GATGAAACAG AGTGCCAAAT ATGGATGTCT GTGCTTCAGA ACAGCAAGGA AGAAGCCTTA 
AACAATGCAT TCAAGGGAGA CCACAGTGCA GGGGAGAACA ACATTGTTCA GGAACTCACT 
AAGGAAATAA TATCTGATAT CCAGAGAATG CCAGGGAACG ACGTGTGCTG TGACTGTGGT 
GCATCAGATC CAACTTGGCT TTCCACAAAC CTGGGGATCC TTACATGCAT TGAGTGCTCT 
GGGATACACA GAGAGCTGGG CGTGCATTAC TCCAGAATAC AGTCTCTTAC ACTGGATGTC 
TTGGGAACTT CTGAGCTTTT GCTTGCCAAA AACATTGGGA ACTCAGGTTT TAATGAACTA 
ATGGAAGCCT GTCTGCCTGC TGATGATTCC GTGAAGCCTT CACCTTGCAG TGACATGAAT 
GCACGGAAAG ACTATATCAC CACCAAGTAC ATAGAAAGAA AGTACGCAAG GAGAAAATAC 
CATGACAATG CCTCTAAACT GCATGGCTTG TGTGACGCCG TCAAATCCAG AGACATCTTT 
ACACTGGTAC AGTTACATGC TGAAGGGGTG GATCTCACCG AGGGCATACC ACTTGCCAAT 
GGACACGAAC AAGGTGAGAC TGCACTCCAT CTCGCTGTCA GATCAGTTGA CCGCAATTCC 
TTGCACATAG TCGACTTTCT TGTACAGAAT AGTGGCAACT TGGACAAACA GACAGCGAAA 
GGAAGTACTG CTCTTCATTA CTGCTGCTTA ACAGACAATG CCGAGTGCCT TAAACTGCTG 
CTGCGAGGCA AAGCCTCCAC AGACATAGCT AACGATGCAG GAGAAACGCC GTTGGACATA 
GCGAAAAGGC TCAAGCATGC ACAGTGCGAG GATCTGCTGA TACAAGCCAT GAATGGAAGG 
TTTAACGCTC ACGTTCATGT GGAATATGAA TGGAGGCTGC ATCACATGGA CCTAGATGAA 
AGCGATGACG ACATGGATGA AAAAATACAG CAGCCAAGCC CTATCCGCAG AGAGGAGAGG 
CCTGTCAGTT TCTTTGGAAT AAACCAGGGC CAGCCTAGTG TTGCCTGCTT AGCCAGGGAA 
GCAGCCAGTA TTGCTAAGGA CAAGCCAAGG ACTTTCCTGC CAAATATCAT TCAGAATGAA 
ACCTATGGAG CAGTCATTGG TAACAGCAAC TCCCCAGGCA ATGCTACAGG TCCTTCTACT 
ACCAATGCTC CTCCTTTGCC TCCCCGAAAC ACTATAAAAG TTCATTCAAC TATATTTGGC 
AGTAGCATAC ACCCCACACC TTCCAACACC CCAGCCTGGA AAGCTAGCTC AGTAACACCG 
GAAAGTGGCA GCAGACAGAG GTCCTCATCT GATCCGCCAT CTATACAGCC CCCTGTGCCA 
CCTGTCCGCG TAACATCCAC TGCCACAAAT TCACCGCCAC CCCCTCCGGT CACTAAGACA 
GCCAGTGTTA TTGAAGCCCT TAGCCAGCAC TCCAAGCAGA GCCCTCCACC TCCAGTTCCG 
CTGAACAAGC CTGTCCCTCC GCCGGTCCCA CCTCAGCCTG CCAACCGCCC CTTACACAAA 
AGGCCGGCTC CTGGGATTGA CAAACCCCCT CCAACAGCCA AGGGGCCACC TCCTGCTTTG 
GTGACTGCGC CACCGTCTGC TGCAGAATTC CCTTCAGACA CAACAGCCTT GCAGCCTGGA 
TCCAAACCCA GCAACCGAAC ACAGCCACCA GCACCCATGC CACGGAAATC CCAGCTGCCC 
AAAATGAAGC CCAAGAGGGT GAAAGCAATA TACCACTGTT CAGCTGACAA TCCGGATGAG 
CTGACCTTCT CAGAGGGTGA AATTATTGTG GTTGATGGGG AGGAAGATAA GGAGTGGTGG 
ATTGGACACA TAGACGGAGA GCCAAACCGA AGAGGTGCCT TCCCTGTGTC GTTTGTGCAC 
TTTATCGCTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914535.2
CDS	416..3490
Translation	MPDLISVSEFVCETNEDYKSPTASNFTNRLSQCRNTVVAIDEALEVDHTILNKMKKSVKAIHTSGLTHVENEEQYTQALEKFGSNCVCRDDPDLGTAFLKFSVFTKELTALFKNLVQNMNNIITFPLDSLLKGDLKGVKGELKKPFEKAWKDYETKVTKIEKEKKEHAKMHGMIRTEISGAEIAEEMEKERRVFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVETLKPSIEKLSTDLHTIKQVQDEERKQLSQLRDILKAALQVEQKEDSQIRQSVAYSLHQPQGNKEHGSERDGYLYKKSDGIRKVWQKRKCTVQNGFLTISHGTANIPPAKLNLLTCQVKTNPEEKKSFDLISHDRTYHFQAEDETECQIWMSVLQNSKEEALNNAFKGDHSAGENNIVQELTKEIISDIQRMPGNDVCCDCGASDPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLGTSELLLAKNIGNSGFNELMEACLPADDSVKPSPCSDMNARKDYITTKYIERKYARRKYHDNASKLHGLCDAVKSRDIFTLVQLHAEGVDLTEGIPLANGHEQGETALHLAVRSVDRNSLHIVDFLVQNSGNLDKQTAKGSTALHYCCLTDNAECLKLLLRGKASTDIANDAGETPLDIAKRLKHAQCEDLLIQAMNGRFNAHVHVEYEWRLHHMDLDESDDDMDEKIQQPSPIRREERPVSFFGINQGQPSVACLAREAASIAKDKPRTFLPNIIQNETYGAVIGNSNSPGNATGPSTTNAPPLPPRNTIKVHSTIFGSSIHPTPSNTPAWKASSVTPESGSRQRSSSDPPSIQPPVPPVRVTSTATNSPPPPPVTKTASVIEALSQHSKQSPPPPVPLNKPVPPPVPPQPANRPLHKRPAPGIDKPPPTAKGPPPALVTAPPSAAEFPSDTTALQPGSKPSNRTQPPAPMPRKSQLPKMKPKRVKAIYHCSADNPDELTFSEGEIIVVDGEEDKEWWIGHIDGEPNRRGAFPVSFVHFIAE

Target ORF information:

RefSeq Version	XM_004914478.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914478.4

ORF Insert Sequence:

ATGCCGGATC TGATCAGTGT GTCGGAATTT GTGTGTGAGA CCAATGAGGA TTACAAGTCC 
CCCACTGCCT CCAATTTCAC CAACAGGCTG AGCCAGTGCA GGAACACGGT CGTGGCTATA 
GATGAGGCTC TGGAGGTTGA TCACACGATT CTGAACAAAA TGAAGAAATC AGTCAAAGCC 
ATTCACACTT CTGGTCTGAC CCACGTGGAA AATGAGGAGC AGTACACTCA AGCACTGGAA 
AAATTTGGAA GCAACTGTGT TTGTCGGGAT GACCCTGATT TGGGAACAGC CTTCTTAAAA 
TTCTCCGTCT TTACCAAGGA GCTAACAGCA CTTTTCAAGA ACCTGGTACA GAACATGAAT 
AACATTATCA CATTTCCACT GGACAGCTTA CTGAAGGGAG ATCTTAAAGG GGTAAAAGGG 
GAATTGAAAA AGCCATTCGA AAAAGCCTGG AAAGACTATG AAACTAAAGT GACCAAAATT 
GAAAAGGAGA AGAAGGAGCA CGCCAAGATG CATGGTATGA TTCGCACTGA GATCAGTGGG 
GCTGAAATTG CTGAGGAAAT GGAGAAAGAA AGACGGGTTT TCCAGCTCCA AATGTGTGAG 
TACCTGCTGA AAGTGAATGA AATAAAGATT AAAAAAGGAG TAGATCTGCT GCAGAACCTG 
ATTAAGTACT TCCACGCACA GTGCAACTTT TTCCAGGATG GATTGAAAGC TGTTGAGACT 
CTGAAGCCCT CTATTGAAAA ACTTTCAACC GATCTGCACA CGATCAAGCA AGTCCAGGAT 
GAAGAGAGGA AGCAGCTTAG CCAGCTAAGG GATATCTTAA AAGCTGCCCT ACAAGTGGAG 
CAGAAGGAGG ATTCACAGAT CCGACAGAGC GTCGCTTACA GCTTGCATCA GCCACAGGGA 
AACAAGGAAC ACGGCTCTGA GAGGGACGGC TATCTCTATA AGAAGAGTGA TGGAATCAGA 
AAGGTTTGGC AGAAGAGGAA ATGCACAGTA CAAAATGGCT TTCTAACCAT ATCTCATGGC 
ACAGCAAACA TTCCGCCTGC AAAGCTGAAC CTCTTAACCT GTCAAGTGAA AACCAACCCA 
GAGGAAAAGA AGAGCTTTGA CCTTATCTCA CATGATCGGA CATATCACTT TCAAGCAGAG 
GATGAAACAG AGTGCCAAAT ATGGATGTCT GTGCTTCAGA ACAGCAAGGA AGAAGCCTTA 
AACAATGCAT TCAAGGGAGA CCACAGTGCA GGGGAGAACA ACATTGTTCA GGAACTCACT 
AAGGAAATAA TATCTGATAT CCAGAGAATG CCAGGGAACG ACGTGTGCTG TGACTGTGGT 
GCATCAGATC CAACTTGGCT TTCCACAAAC CTGGGGATCC TTACATGCAT TGAGTGCTCT 
GGGATACACA GAGAGCTGGG CGTGCATTAC TCCAGAATAC AGTCTCTTAC ACTGGATGTC 
TTGGGAACTT CTGAGCTTTT GCTTGCCAAA AACATTGGGA ACTCAGGTTT TAATGAACTA 
ATGGAAGCCT GTCTGCCTGC TGATGATTCC GTGAAGCCTT CACCTTGCAG TGACATGAAT 
GCACGGAAAG ACTATATCAC CACCAAGTAC ATAGAAAGAA AGTACGCAAG GAGAAAATAC 
CATGACAATG CCTCTAAACT GCATGGCTTG TGTGACGCCG TCAAATCCAG AGACATCTTT 
ACACTGGTAC AGTTACATGC TGAAGGGGTG GATCTCACCG AGGGCATACC ACTTGCCAAT 
GGACACGAAC AAGGTGAGAC TGCACTCCAT CTCGCTGTCA GATCAGTTGA CCGCAATTCC 
TTGCACATAG TCGACTTTCT TGTACAGAAT AGTGGCAACT TGGACAAACA GACAGCGAAA 
GGAAGTACTG CTCTTCATTA CTGCTGCTTA ACAGACAATG CCGAGTGCCT TAAACTGCTG 
CTGCGAGGCA AAGCCTCCAC AGACATAGCT AACGATGCAG GAGAAACGCC GTTGGACATA 
GCGAAAAGGC TCAAGCATGC ACAGTGCGAG GATCTGCTGA TACAAGCCAT GAATGGAAGG 
TTTAACGCTC ACGTTCATGT GGAATATGAA TGGAGGCTGC ATCACATGGA CCTAGATGAA 
AGCGATGACG ACATGGATGA AAAAATACAG CAGCCAAGCC CTATCCGCAG AGAGGAGAGG 
CCTGTCAGTT TCTTTGGAAT AAACCAGGGC CAGCCTAGTG TTGCCTGCTT AGCCAGGGAA 
GCAGCCAGTA TTGCTAAGGA CAAGCCAAGG ACTTTCCTGC CAAATATCAT TCAGAATGAA 
ACCTATGGAG CAGTCATTGG TAACAGCAAC TCCCCAGGCA ATGCTACAGG TCCTTCTACT 
ACCAATGCTC CTCCTTTGCC TCCCCGAAAC ACTATAAAAG TTCATTCAAC TATATTTGGC 
AGTAGCATAC ACCCCACACC TTCCAACACC CCAGCCTGGA AAGCTAGCTC AGTAACACCG 
GAAAGTGGCA GCAGACAGAG GTCCTCATCT GATCCGCCAT CTATACAGCC CCCTGTGCCA 
CCTGTCCGCG TAACATCCAC TGCCACAAAT TCACCGCCAC CCCCTCCGGT CACTAAGACA 
GCCAGTGTTA TTGAAGCCCT TAGCCAGCAC TCCAAGCAGA GCCCTCCACC TCCAGTTCCG 
CTGAACAAGC CTGTCCCTCC GCCGGTCCCA CCTCAGCCTG CCAACCGCCC CTTACACAAA 
AGGCCGGCTC CTGGGATTGA CAAACCCCCT CCAACAGCCA AGGGGCCACC TCCTGCTTTG 
GTGACTGCGC CACCGTCTGC TGCAGAATTC CCTTCAGACA CAACAGCCTT GCAGCCTGGA 
TCCAAACCCA GCAACCGAAC ACAGCCACCA GCACCCATGC CACGGAAATC CCAGCTGCCC 
AAAATGAAGC CCAAGAGGGT GAAAGCAATA TACCACTGTT CAGCTGACAA TCCGGATGAG 
CTGACCTTCT CAGAGGGTGA AATTATTGTG GTTGATGGGG AGGAAGATAA GGAGTGGTGG 
ATTGGACACA TAGACGGAGA GCCAAACCGA AGAGGTGCCT TCCCTGTGTC GTTTGTGCAC 
TTTATCGCTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2 ( XM_002935032.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935078.2 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa10335
Clone ID Related Accession (Same CDS sequence)	XM_002935032.4
Accession Version	XM_002935032.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935032.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGATC TGATCAGTGT GTCGGAATTT GTGTGTGAGA CCAATGAGGA TTACAAGTCC 
CCCACTGCCT CCAATTTCAC CAACAGGCTG AGCCAGTGCA GGAACACGGT CGTGGCTATA 
GATGAGGCTC TGGAGGTTGA TCACACGATT CTGAACAAAA TGAAGAAATC AGTCAAAGCC 
ATTCACACTT CTGGTCTGAC CCACGTGGAA AATGAGGAGC AGTACACTCA AGCACTGGAA 
AAATTTGGAA GCAACTGTGT TTGTCGGGAT GACCCTGATT TGGGAACAGC CTTCTTAAAA 
TTCTCCGTCT TTACCAAGGA GCTAACAGCA CTTTTCAAGA ACCTGGTACA GAACATGAAT 
AACATTATCA CATTTCCACT GGACAGCTTA CTGAAGGGAG ATCTTAAAGG GGTAAAAGGG 
GAATTGAAAA AGCCATTCGA AAAAGCCTGG AAAGACTATG AAACTAAAGT GACCAAAATT 
GAAAAGGAGA AGAAGGAGCA CGCCAAGATG CATGGTATGA TTCGCACTGA GATCAGTGGG 
GCTGAAATTG CTGAGGAAAT GGAGAAAGAA AGACGGGTTT TCCAGCTCCA AATGTGTGAG 
TACCTGCTGA AAGTGAATGA AATAAAGATT AAAAAAGGAG TAGATCTGCT GCAGAACCTG 
ATTAAGTACT TCCACGCACA GTGCAACTTT TTCCAGGATG GATTGAAAGC TGTTGAGACT 
CTGAAGCCCT CTATTGAAAA ACTTTCAACC GATCTGCACA CGATCAAGCA AGTCCAGGAT 
GAAGAGAGGA AGCAGCTTAG CCAGCTAAGG GATATCTTAA AAGCTGCCCT ACAAGTGGAG 
CAGAAGGAGG ATTCACAGAT CCGACAGAGC GTCGCTTACA GCTTGCATCA GCCACAGGGA 
AACAAGGAAC ACGGCTCTGA GAGGGACGGC TATCTCTATA AGAAGAGTGA TGGAATCAGA 
AAGGTTTGGC AGAAGAGGAA ATGCACAGTA CAAAATGGCT TTCTAACCAT ATCTCATGGC 
ACAGCAAACA TTCCGCCTGC AAAGCTGAAC CTCTTAACCT GTCAAGTGAA AACCAACCCA 
GAGGAAAAGA AGAGCTTTGA CCTTATCTCA CATGATCGGA CATATCACTT TCAAGCAGAG 
GATGAAACAG AGTGCCAAAT ATGGATGTCT GTGCTTCAGA ACAGCAAGGA AGAAGCCTTA 
AACAATGCAT TCAAGGGAGA CCACAGTGCA GGGGAGAACA ACATTGTTCA GGAACTCACT 
AAGGAAATAA TATCTGATAT CCAGAGAATG CCAGGGAACG ACGTGTGCTG TGACTGTGGT 
GCATCAGATC CAACTTGGCT TTCCACAAAC CTGGGGATCC TTACATGCAT TGAGTGCTCT 
GGGATACACA GAGAGCTGGG CGTGCATTAC TCCAGAATAC AGTCTCTTAC ACTGGATGTC 
TTGGGAACTT CTGAGCTTTT GCTTGCCAAA AACATTGGGA ACTCAGGTTT TAATGAACTA 
ATGGAAGCCT GTCTGCCTGC TGATGATTCC GTGAAGCCTT CACCTTGCAG TGACATGAAT 
GCACGGAAAG ACTATATCAC CACCAAGTAC ATAGAAAGAA AGTACGCAAG GAGAAAATAC 
CATGACAATG CCTCTAAACT GCATGGCTTG TGTGACGCCG TCAAATCCAG AGACATCTTT 
ACACTGGTAC AGTTACATGC TGAAGGGGTG GATCTCACCG AGGGCATACC ACTTGCCAAT 
GGACACGAAC AAGGTGAGAC TGCACTCCAT CTCGCTGTCA GATCAGTTGA CCGCAATTCC 
TTGCACATAG TCGACTTTCT TGTACAGAAT AGTGGCAACT TGGACAAACA GACAGCGAAA 
GGAAGTACTG CTCTTCATTA CTGCTGCTTA ACAGACAATG CCGAGTGCCT TAAACTGCTG 
CTGCGAGGCA AAGCCTCCAC AGACATAGCT AACGATGCAG GAGAAACGCC GTTGGACATA 
GCGAAAAGGC TCAAGCATGC ACAGTGCGAG GATCTGCTGA TACAAGCCAT GAATGGAAGG 
TTTAACGCTC ACGTTCATGT GGAATATGAA TGGAGGCTGC ATCACATGGA CCTAGATGAA 
AGCGATGACG ACATGGATGA AAAAATACAG CAGCCAAGCC CTATCCGCAG AGAGGAGAGG 
CCTGTCAGTT TCTTTGGAAT AAACCAGGGC CAGCCTAGTG TTGCCTGCTT AGCCAGGGAA 
GCAGCCAGTA TTGCTAAGGA CAAGCCAAGG ACTTTCCTGC CAAATATCAT TCAGAATGAA 
ACCTATGGAG CAGTCATTGG TAACAGCAAC TCCCCAGGCA ATGCTACAGG TCCTTCTACT 
ACCAATGCTC CTCCTTTGCC TCCCCGAAAC ACTATAAAAG TTCATTCAAC TATATTTGGC 
AGTAGCATAC ACCCCACACC TTCCAACACC CCAGCCTGGA AAGCTAGCTC AGTAACACCG 
GAAAGTGGCA GCAGACAGAG GTCCTCATCT GATCCGCCAT CTATACAGCC CCCTGTGCCA 
CCTGTCCGCG TAACATCCAC TGCCACAAAT TCACCGCCAC CCCCTCCGGT CACTAAGACA 
GCCAGTGTTA TTGAAGCCCT TAGCCAGCAC TCCAAGCAGA GCCCTCCACC TCCAGTTCCG 
CTGAACAAGC CTGTCCCTCC GCCGGTCCCA CCTCAGCCTG CCAACCGCCC CTTACACAAA 
AGGCCGGCTC CTGGGATTGA CAAACCCCCT CCAACAGCCA AGGGGCCACC TCCTGCTTTG 
GTGACTGCGC CACCGTCTGC AGCTGCAGAA TTCCCTTCAG ACACAACAGC CTTGCAGCCT 
GGATCCAAAC CCAGCAACCG AACACAGCCA CCAGCACCCA TGCCACGGAA ATCCCAGCTG 
CCCAAAATGA AGCCCAAGAG GGTGAAAGCA ATATACCACT GTTCAGCTGA CAATCCGGAT 
GAGCTGACCT TCTCAGAGGG TGAAATTATT GTGGTTGATG GGGAGGAAGA TAAGGAGTGG 
TGGATTGGAC ACATAGACGG AGAGCCAACT CGAAGAGGTG CCTTCCCTGT GTCGTTTGTG 
CACTTTATCG CTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935078.2
CDS	431..3508
Translation	MPDLISVSEFVCETNEDYKSPTASNFTNRLSQCRNTVVAIDEALEVDHTILNKMKKSVKAIHTSGLTHVENEEQYTQALEKFGSNCVCRDDPDLGTAFLKFSVFTKELTALFKNLVQNMNNIITFPLDSLLKGDLKGVKGELKKPFEKAWKDYETKVTKIEKEKKEHAKMHGMIRTEISGAEIAEEMEKERRVFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVETLKPSIEKLSTDLHTIKQVQDEERKQLSQLRDILKAALQVEQKEDSQIRQSVAYSLHQPQGNKEHGSERDGYLYKKSDGIRKVWQKRKCTVQNGFLTISHGTANIPPAKLNLLTCQVKTNPEEKKSFDLISHDRTYHFQAEDETECQIWMSVLQNSKEEALNNAFKGDHSAGENNIVQELTKEIISDIQRMPGNDVCCDCGASDPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLGTSELLLAKNIGNSGFNELMEACLPADDSVKPSPCSDMNARKDYITTKYIERKYARRKYHDNASKLHGLCDAVKSRDIFTLVQLHAEGVDLTEGIPLANGHEQGETALHLAVRSVDRNSLHIVDFLVQNSGNLDKQTAKGSTALHYCCLTDNAECLKLLLRGKASTDIANDAGETPLDIAKRLKHAQCEDLLIQAMNGRFNAHVHVEYEWRLHHMDLDESDDDMDEKIQQPSPIRREERPVSFFGINQGQPSVACLAREAASIAKDKPRTFLPNIIQNETYGAVIGNSNSPGNATGPSTTNAPPLPPRNTIKVHSTIFGSSIHPTPSNTPAWKASSVTPESGSRQRSSSDPPSIQPPVPPVRVTSTATNSPPPPPVTKTASVIEALSQHSKQSPPPPVPLNKPVPPPVPPQPANRPLHKRPAPGIDKPPPTAKGPPPALVTAPPSAAAEFPSDTTALQPGSKPSNRTQPPAPMPRKSQLPKMKPKRVKAIYHCSADNPDELTFSEGEIIVVDGEEDKEWWIGHIDGEPTRRGAFPVSFVHFIAE

Target ORF information:

RefSeq Version	XM_002935032.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935032.4

ORF Insert Sequence:

ATGCCGGATC TGATCAGTGT GTCGGAATTT GTGTGTGAGA CCAATGAGGA TTACAAGTCC 
CCCACTGCCT CCAATTTCAC CAACAGGCTG AGCCAGTGCA GGAACACGGT CGTGGCTATA 
GATGAGGCTC TGGAGGTTGA TCACACGATT CTGAACAAAA TGAAGAAATC AGTCAAAGCC 
ATTCACACTT CTGGTCTGAC CCACGTGGAA AATGAGGAGC AGTACACTCA AGCACTGGAA 
AAATTTGGAA GCAACTGTGT TTGTCGGGAT GACCCTGATT TGGGAACAGC CTTCTTAAAA 
TTCTCCGTCT TTACCAAGGA GCTAACAGCA CTTTTCAAGA ACCTGGTACA GAACATGAAT 
AACATTATCA CATTTCCACT GGACAGCTTA CTGAAGGGAG ATCTTAAAGG GGTAAAAGGG 
GAATTGAAAA AGCCATTCGA AAAAGCCTGG AAAGACTATG AAACTAAAGT GACCAAAATT 
GAAAAGGAGA AGAAGGAGCA CGCCAAGATG CATGGTATGA TTCGCACTGA GATCAGTGGG 
GCTGAAATTG CTGAGGAAAT GGAGAAAGAA AGACGGGTTT TCCAGCTCCA AATGTGTGAG 
TACCTGCTGA AAGTGAATGA AATAAAGATT AAAAAAGGAG TAGATCTGCT GCAGAACCTG 
ATTAAGTACT TCCACGCACA GTGCAACTTT TTCCAGGATG GATTGAAAGC TGTTGAGACT 
CTGAAGCCCT CTATTGAAAA ACTTTCAACC GATCTGCACA CGATCAAGCA AGTCCAGGAT 
GAAGAGAGGA AGCAGCTTAG CCAGCTAAGG GATATCTTAA AAGCTGCCCT ACAAGTGGAG 
CAGAAGGAGG ATTCACAGAT CCGACAGAGC GTCGCTTACA GCTTGCATCA GCCACAGGGA 
AACAAGGAAC ACGGCTCTGA GAGGGACGGC TATCTCTATA AGAAGAGTGA TGGAATCAGA 
AAGGTTTGGC AGAAGAGGAA ATGCACAGTA CAAAATGGCT TTCTAACCAT ATCTCATGGC 
ACAGCAAACA TTCCGCCTGC AAAGCTGAAC CTCTTAACCT GTCAAGTGAA AACCAACCCA 
GAGGAAAAGA AGAGCTTTGA CCTTATCTCA CATGATCGGA CATATCACTT TCAAGCAGAG 
GATGAAACAG AGTGCCAAAT ATGGATGTCT GTGCTTCAGA ACAGCAAGGA AGAAGCCTTA 
AACAATGCAT TCAAGGGAGA CCACAGTGCA GGGGAGAACA ACATTGTTCA GGAACTCACT 
AAGGAAATAA TATCTGATAT CCAGAGAATG CCAGGGAACG ACGTGTGCTG TGACTGTGGT 
GCATCAGATC CAACTTGGCT TTCCACAAAC CTGGGGATCC TTACATGCAT TGAGTGCTCT 
GGGATACACA GAGAGCTGGG CGTGCATTAC TCCAGAATAC AGTCTCTTAC ACTGGATGTC 
TTGGGAACTT CTGAGCTTTT GCTTGCCAAA AACATTGGGA ACTCAGGTTT TAATGAACTA 
ATGGAAGCCT GTCTGCCTGC TGATGATTCC GTGAAGCCTT CACCTTGCAG TGACATGAAT 
GCACGGAAAG ACTATATCAC CACCAAGTAC ATAGAAAGAA AGTACGCAAG GAGAAAATAC 
CATGACAATG CCTCTAAACT GCATGGCTTG TGTGACGCCG TCAAATCCAG AGACATCTTT 
ACACTGGTAC AGTTACATGC TGAAGGGGTG GATCTCACCG AGGGCATACC ACTTGCCAAT 
GGACACGAAC AAGGTGAGAC TGCACTCCAT CTCGCTGTCA GATCAGTTGA CCGCAATTCC 
TTGCACATAG TCGACTTTCT TGTACAGAAT AGTGGCAACT TGGACAAACA GACAGCGAAA 
GGAAGTACTG CTCTTCATTA CTGCTGCTTA ACAGACAATG CCGAGTGCCT TAAACTGCTG 
CTGCGAGGCA AAGCCTCCAC AGACATAGCT AACGATGCAG GAGAAACGCC GTTGGACATA 
GCGAAAAGGC TCAAGCATGC ACAGTGCGAG GATCTGCTGA TACAAGCCAT GAATGGAAGG 
TTTAACGCTC ACGTTCATGT GGAATATGAA TGGAGGCTGC ATCACATGGA CCTAGATGAA 
AGCGATGACG ACATGGATGA AAAAATACAG CAGCCAAGCC CTATCCGCAG AGAGGAGAGG 
CCTGTCAGTT TCTTTGGAAT AAACCAGGGC CAGCCTAGTG TTGCCTGCTT AGCCAGGGAA 
GCAGCCAGTA TTGCTAAGGA CAAGCCAAGG ACTTTCCTGC CAAATATCAT TCAGAATGAA 
ACCTATGGAG CAGTCATTGG TAACAGCAAC TCCCCAGGCA ATGCTACAGG TCCTTCTACT 
ACCAATGCTC CTCCTTTGCC TCCCCGAAAC ACTATAAAAG TTCATTCAAC TATATTTGGC 
AGTAGCATAC ACCCCACACC TTCCAACACC CCAGCCTGGA AAGCTAGCTC AGTAACACCG 
GAAAGTGGCA GCAGACAGAG GTCCTCATCT GATCCGCCAT CTATACAGCC CCCTGTGCCA 
CCTGTCCGCG TAACATCCAC TGCCACAAAT TCACCGCCAC CCCCTCCGGT CACTAAGACA 
GCCAGTGTTA TTGAAGCCCT TAGCCAGCAC TCCAAGCAGA GCCCTCCACC TCCAGTTCCG 
CTGAACAAGC CTGTCCCTCC GCCGGTCCCA CCTCAGCCTG CCAACCGCCC CTTACACAAA 
AGGCCGGCTC CTGGGATTGA CAAACCCCCT CCAACAGCCA AGGGGCCACC TCCTGCTTTG 
GTGACTGCGC CACCGTCTGC AGCTGCAGAA TTCCCTTCAG ACACAACAGC CTTGCAGCCT 
GGATCCAAAC CCAGCAACCG AACACAGCCA CCAGCACCCA TGCCACGGAA ATCCCAGCTG 
CCCAAAATGA AGCCCAAGAG GGTGAAAGCA ATATACCACT GTTCAGCTGA CAATCCGGAT 
GAGCTGACCT TCTCAGAGGG TGAAATTATT GTGGTTGATG GGGAGGAAGA TAAGGAGTGG 
TGGATTGGAC ACATAGACGG AGAGCCAACT CGAAGAGGTG CCTTCCCTGT GTCGTTTGTG 
CACTTTATCG CTGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2 ( XM_004914478.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914535.1 Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa10336
Clone ID Related Accession (Same CDS sequence)	XM_004914478.3
Accession Version	XM_004914478.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914478.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGATC TGATCAGTGT GTCGGAATTT GTGTGTGAGA CCAATGAGGA TTACAAGTCC 
CCCACTGCCT CCAATTTCAC CAACAGGCTG AGCCAGTGCA GGAACACGGT CGTGGCTATA 
GATGAGGCTC TGGAGGTTGA TCACACGATT CTGAACAAAA TGAAGAAATC AGTCAAAGCC 
ATTCACACTT CTGGTCTGAC CCACGTGGAA AATGAGGAGC AGTACACTCA AGCACTGGAA 
AAATTTGGAA GCAACTGTGT TTGTCGGGAT GACCCTGATT TGGGAACAGC CTTCTTAAAA 
TTCTCCGTCT TTACCAAGGA GCTAACAGCA CTTTTCAAGA ACCTGGTACA GAACATGAAT 
AACATTATCA CATTTCCACT GGACAGCTTA CTGAAGGGAG ATCTTAAAGG GGTAAAAGGG 
GAATTGAAAA AGCCATTCGA AAAAGCCTGG AAAGACTATG AAACTAAAGT GACCAAAATT 
GAAAAGGAGA AGAAGGAGCA CGCCAAGATG CATGGTATGA TTCGCACTGA GATCAGTGGG 
GCTGAAATTG CTGAGGAAAT GGAGAAAGAA AGACGGGTTT TCCAGCTCCA AATGTGTGAG 
TACCTGCTGA AAGTGAATGA AATAAAGATT AAAAAAGGAG TAGATCTGCT GCAGAACCTG 
ATTAAGTACT TCCACGCACA GTGCAACTTT TTCCAGGATG GATTGAAAGC TGTTGAGACT 
CTGAAGCCCT CTATTGAAAA ACTTTCAACC GATCTGCACA CGATCAAGCA AGTCCAGGAT 
GAAGAGAGGA AGCAGCTTAG CCAGCTAAGG GATATCTTAA AAGCTGCCCT ACAAGTGGAG 
CAGAAGGAGG ATTCACAGAT CCGACAGAGC GTCGCTTACA GCTTGCATCA GCCACAGGGA 
AACAAGGAAC ACGGCTCTGA GAGGGACGGC TATCTCTATA AGAAGAGTGA TGGAATCAGA 
AAGGTTTGGC AGAAGAGGAA ATGCACAGTA CAAAATGGCT TTCTAACCAT ATCTCATGGC 
ACAGCAAACA TTCCGCCTGC AAAGCTGAAC CTCTTAACCT GTCAAGTGAA AACCAACCCA 
GAGGAAAAGA AGAGCTTTGA CCTTATCTCA CATGATCGGA CATATCACTT TCAAGCAGAG 
GATGAAACAG AGTGCCAAAT ATGGATGTCT GTGCTTCAGA ACAGCAAGGA AGAAGCCTTA 
AACAATGCAT TCAAGGGAGA CCACAGTGCA GGGGAGAACA ACATTGTTCA GGAACTCACT 
AAGGAAATAA TATCTGATAT CCAGAGAATG CCAGGGAACG ACGTGTGCTG TGACTGTGGT 
GCATCAGATC CAACTTGGCT TTCCACAAAC CTGGGGATCC TTACATGCAT TGAGTGCTCT 
GGGATACACA GAGAGCTGGG CGTGCATTAC TCCAGAATAC AGTCTCTTAC ACTGGATGTC 
TTGGGAACTT CTGAGCTTTT GCTTGCCAAA AACATTGGGA ACTCAGGTTT TAATGAACTA 
ATGGAAGCCT GTCTGCCTGC TGATGATTCC GTGAAGCCTT CACCTTGCAG TGACATGAAT 
GCACGGAAAG ACTATATCAC CACCAAGTAC ATAGAAAGAA AGTACGCAAG GAGAAAATAC 
CATGACAATG CCTCTAAACT GCATGGCTTG TGTGACGCCG TCAAATCCAG AGACATCTTT 
ACACTGGTAC AGTTACATGC TGAAGGGGTG GATCTCACCG AGGGCATACC ACTTGCCAAT 
GGACACGAAC AAGGTGAGAC TGCACTCCAT CTCGCTGTCA GATCAGTTGA CCGCAATTCC 
TTGCACATAG TCGACTTTCT TGTACAGAAT AGTGGCAACT TGGACAAACA GACAGCGAAA 
GGAAGTACTG CTCTTCATTA CTGCTGCTTA ACAGACAATG CCGAGTGCCT TAAACTGCTG 
CTGCGAGGCA AAGCCTCCAC AGACATAGCT AACGATGCAG GAGAAACGCC GTTGGACATA 
GCGAAAAGGC TCAAGCATGC ACAGTGCGAG GATCTGCTGA TACAAGCCAT GAATGGAAGG 
TTTAACGCTC ACGTTCATGT GGAATATGAA TGGAGGCTGC ATCACATGGA CCTAGATGAA 
AGCGATGACG ACATGGATGA AAAAATACAG CAGCCAAGCC CTATCCGCAG AGAGGAGAGG 
CCTGTCAGTT TCTTTGGAAT AAACCAGGGC CAGCCTAGTG TTGCCTGCTT AGCCAGGGAA 
GCAGCCAGTA TTGCTAAGGA CAAGCCAAGG ACTTTCCTGC CAAATATCAT TCAGAATGAA 
ACCTATGGAG CAGTCATTGG TAACAGCAAC TCCCCAGGCA ATGCTACAGG TCCTTCTACT 
ACCAATGCTC CTCCTTTGCC TCCCCGAAAC ACTATAAAAG TTCATTCAAC TATATTTGGC 
AGTAGCATAC ACCCCACACC TTCCAACACC CCAGCCTGGA AAGCTAGCTC AGTAACACCG 
GAAAGTGGCA GCAGACAGAG GTCCTCATCT GATCCGCCAT CTATACAGCC CCCTGTGCCA 
CCTGTCCGCG TAACATCCAC TGCCACAAAT TCACCGCCAC CCCCTCCGGT CACTAAGACA 
GCCAGTGTTA TTGAAGCCCT TAGCCAGCAC TCCAAGCAGA GCCCTCCACC TCCAGTTCCG 
CTGAACAAGC CTGTCCCTCC GCCGGTCCCA CCTCAGCCTG CCAACCGCCC CTTACACAAA 
AGGCCGGCTC CTGGGATTGA CAAACCCCCT CCAACAGCCA AGGGGCCACC TCCTGCTTTG 
GTGACTGCGC CACCGTCTGC TGCAGAATTC CCTTCAGACA CAACAGCCTT GCAGCCTGGA 
TCCAAACCCA GCAACCGAAC ACAGCCACCA GCACCCATGC CACGGAAATC CCAGCTGCCC 
AAAATGAAGC CCAAGAGGGT GAAAGCAATA TACCACTGTT CAGCTGACAA TCCGGATGAG 
CTGACCTTCT CAGAGGGTGA AATTATTGTG GTTGATGGGG AGGAAGATAA GGAGTGGTGG 
ATTGGACACA TAGACGGAGA GCCAACTCGA AGAGGTGCCT TCCCTGTGTC GTTTGTGCAC 
TTTATCGCTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914535.1
CDS	431..3505
Translation	MPDLISVSEFVCETNEDYKSPTASNFTNRLSQCRNTVVAIDEALEVDHTILNKMKKSVKAIHTSGLTHVENEEQYTQALEKFGSNCVCRDDPDLGTAFLKFSVFTKELTALFKNLVQNMNNIITFPLDSLLKGDLKGVKGELKKPFEKAWKDYETKVTKIEKEKKEHAKMHGMIRTEISGAEIAEEMEKERRVFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVETLKPSIEKLSTDLHTIKQVQDEERKQLSQLRDILKAALQVEQKEDSQIRQSVAYSLHQPQGNKEHGSERDGYLYKKSDGIRKVWQKRKCTVQNGFLTISHGTANIPPAKLNLLTCQVKTNPEEKKSFDLISHDRTYHFQAEDETECQIWMSVLQNSKEEALNNAFKGDHSAGENNIVQELTKEIISDIQRMPGNDVCCDCGASDPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLGTSELLLAKNIGNSGFNELMEACLPADDSVKPSPCSDMNARKDYITTKYIERKYARRKYHDNASKLHGLCDAVKSRDIFTLVQLHAEGVDLTEGIPLANGHEQGETALHLAVRSVDRNSLHIVDFLVQNSGNLDKQTAKGSTALHYCCLTDNAECLKLLLRGKASTDIANDAGETPLDIAKRLKHAQCEDLLIQAMNGRFNAHVHVEYEWRLHHMDLDESDDDMDEKIQQPSPIRREERPVSFFGINQGQPSVACLAREAASIAKDKPRTFLPNIIQNETYGAVIGNSNSPGNATGPSTTNAPPLPPRNTIKVHSTIFGSSIHPTPSNTPAWKASSVTPESGSRQRSSSDPPSIQPPVPPVRVTSTATNSPPPPPVTKTASVIEALSQHSKQSPPPPVPLNKPVPPPVPPQPANRPLHKRPAPGIDKPPPTAKGPPPALVTAPPSAAEFPSDTTALQPGSKPSNRTQPPAPMPRKSQLPKMKPKRVKAIYHCSADNPDELTFSEGEIIVVDGEEDKEWWIGHIDGEPTRRGAFPVSFVHFIAE

Target ORF information:

RefSeq Version	XM_004914478.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (asap2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914478.3

ORF Insert Sequence:

ATGCCGGATC TGATCAGTGT GTCGGAATTT GTGTGTGAGA CCAATGAGGA TTACAAGTCC 
CCCACTGCCT CCAATTTCAC CAACAGGCTG AGCCAGTGCA GGAACACGGT CGTGGCTATA 
GATGAGGCTC TGGAGGTTGA TCACACGATT CTGAACAAAA TGAAGAAATC AGTCAAAGCC 
ATTCACACTT CTGGTCTGAC CCACGTGGAA AATGAGGAGC AGTACACTCA AGCACTGGAA 
AAATTTGGAA GCAACTGTGT TTGTCGGGAT GACCCTGATT TGGGAACAGC CTTCTTAAAA 
TTCTCCGTCT TTACCAAGGA GCTAACAGCA CTTTTCAAGA ACCTGGTACA GAACATGAAT 
AACATTATCA CATTTCCACT GGACAGCTTA CTGAAGGGAG ATCTTAAAGG GGTAAAAGGG 
GAATTGAAAA AGCCATTCGA AAAAGCCTGG AAAGACTATG AAACTAAAGT GACCAAAATT 
GAAAAGGAGA AGAAGGAGCA CGCCAAGATG CATGGTATGA TTCGCACTGA GATCAGTGGG 
GCTGAAATTG CTGAGGAAAT GGAGAAAGAA AGACGGGTTT TCCAGCTCCA AATGTGTGAG 
TACCTGCTGA AAGTGAATGA AATAAAGATT AAAAAAGGAG TAGATCTGCT GCAGAACCTG 
ATTAAGTACT TCCACGCACA GTGCAACTTT TTCCAGGATG GATTGAAAGC TGTTGAGACT 
CTGAAGCCCT CTATTGAAAA ACTTTCAACC GATCTGCACA CGATCAAGCA AGTCCAGGAT 
GAAGAGAGGA AGCAGCTTAG CCAGCTAAGG GATATCTTAA AAGCTGCCCT ACAAGTGGAG 
CAGAAGGAGG ATTCACAGAT CCGACAGAGC GTCGCTTACA GCTTGCATCA GCCACAGGGA 
AACAAGGAAC ACGGCTCTGA GAGGGACGGC TATCTCTATA AGAAGAGTGA TGGAATCAGA 
AAGGTTTGGC AGAAGAGGAA ATGCACAGTA CAAAATGGCT TTCTAACCAT ATCTCATGGC 
ACAGCAAACA TTCCGCCTGC AAAGCTGAAC CTCTTAACCT GTCAAGTGAA AACCAACCCA 
GAGGAAAAGA AGAGCTTTGA CCTTATCTCA CATGATCGGA CATATCACTT TCAAGCAGAG 
GATGAAACAG AGTGCCAAAT ATGGATGTCT GTGCTTCAGA ACAGCAAGGA AGAAGCCTTA 
AACAATGCAT TCAAGGGAGA CCACAGTGCA GGGGAGAACA ACATTGTTCA GGAACTCACT 
AAGGAAATAA TATCTGATAT CCAGAGAATG CCAGGGAACG ACGTGTGCTG TGACTGTGGT 
GCATCAGATC CAACTTGGCT TTCCACAAAC CTGGGGATCC TTACATGCAT TGAGTGCTCT 
GGGATACACA GAGAGCTGGG CGTGCATTAC TCCAGAATAC AGTCTCTTAC ACTGGATGTC 
TTGGGAACTT CTGAGCTTTT GCTTGCCAAA AACATTGGGA ACTCAGGTTT TAATGAACTA 
ATGGAAGCCT GTCTGCCTGC TGATGATTCC GTGAAGCCTT CACCTTGCAG TGACATGAAT 
GCACGGAAAG ACTATATCAC CACCAAGTAC ATAGAAAGAA AGTACGCAAG GAGAAAATAC 
CATGACAATG CCTCTAAACT GCATGGCTTG TGTGACGCCG TCAAATCCAG AGACATCTTT 
ACACTGGTAC AGTTACATGC TGAAGGGGTG GATCTCACCG AGGGCATACC ACTTGCCAAT 
GGACACGAAC AAGGTGAGAC TGCACTCCAT CTCGCTGTCA GATCAGTTGA CCGCAATTCC 
TTGCACATAG TCGACTTTCT TGTACAGAAT AGTGGCAACT TGGACAAACA GACAGCGAAA 
GGAAGTACTG CTCTTCATTA CTGCTGCTTA ACAGACAATG CCGAGTGCCT TAAACTGCTG 
CTGCGAGGCA AAGCCTCCAC AGACATAGCT AACGATGCAG GAGAAACGCC GTTGGACATA 
GCGAAAAGGC TCAAGCATGC ACAGTGCGAG GATCTGCTGA TACAAGCCAT GAATGGAAGG 
TTTAACGCTC ACGTTCATGT GGAATATGAA TGGAGGCTGC ATCACATGGA CCTAGATGAA 
AGCGATGACG ACATGGATGA AAAAATACAG CAGCCAAGCC CTATCCGCAG AGAGGAGAGG 
CCTGTCAGTT TCTTTGGAAT AAACCAGGGC CAGCCTAGTG TTGCCTGCTT AGCCAGGGAA 
GCAGCCAGTA TTGCTAAGGA CAAGCCAAGG ACTTTCCTGC CAAATATCAT TCAGAATGAA 
ACCTATGGAG CAGTCATTGG TAACAGCAAC TCCCCAGGCA ATGCTACAGG TCCTTCTACT 
ACCAATGCTC CTCCTTTGCC TCCCCGAAAC ACTATAAAAG TTCATTCAAC TATATTTGGC 
AGTAGCATAC ACCCCACACC TTCCAACACC CCAGCCTGGA AAGCTAGCTC AGTAACACCG 
GAAAGTGGCA GCAGACAGAG GTCCTCATCT GATCCGCCAT CTATACAGCC CCCTGTGCCA 
CCTGTCCGCG TAACATCCAC TGCCACAAAT TCACCGCCAC CCCCTCCGGT CACTAAGACA 
GCCAGTGTTA TTGAAGCCCT TAGCCAGCAC TCCAAGCAGA GCCCTCCACC TCCAGTTCCG 
CTGAACAAGC CTGTCCCTCC GCCGGTCCCA CCTCAGCCTG CCAACCGCCC CTTACACAAA 
AGGCCGGCTC CTGGGATTGA CAAACCCCCT CCAACAGCCA AGGGGCCACC TCCTGCTTTG 
GTGACTGCGC CACCGTCTGC TGCAGAATTC CCTTCAGACA CAACAGCCTT GCAGCCTGGA 
TCCAAACCCA GCAACCGAAC ACAGCCACCA GCACCCATGC CACGGAAATC CCAGCTGCCC 
AAAATGAAGC CCAAGAGGGT GAAAGCAATA TACCACTGTT CAGCTGACAA TCCGGATGAG 
CTGACCTTCT CAGAGGGTGA AATTATTGTG GTTGATGGGG AGGAAGATAA GGAGTGGTGG 
ATTGGACACA TAGACGGAGA GCCAACTCGA AGAGGTGCCT TCCCTGTGTC GTTTGTGCAC 
TTTATCGCTG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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asap2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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