ralgapb gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012952781.2	XP_012808235.1	ral GTPase-activating protein subunit beta isoform X6
XM_002932859.4	XP_002932905.1	ral GTPase-activating protein subunit beta isoform X7
XM_004918518.3	XP_004918575.1	ral GTPase-activating protein subunit beta isoform X8
XM_012952778.3	XP_012808232.2	ral GTPase-activating protein subunit beta isoform X2
XM_012952779.3	XP_012808233.2	ral GTPase-activating protein subunit beta isoform X3
XM_012952780.3	XP_012808234.2	ral GTPase-activating protein subunit beta isoform X4
XM_012952781.3	XP_012808235.2	ral GTPase-activating protein subunit beta isoform X5
XM_002932859.5	XP_002932905.2	ral GTPase-activating protein subunit beta isoform X6
XM_012952779.2	XP_012808233.1	ral GTPase-activating protein subunit beta isoform X4
XM_012952776.3	XP_012808230.2	ral GTPase-activating protein subunit beta isoform X1
XM_012952776.2	XP_012808230.1	ral GTPase-activating protein subunit beta isoform X1
XM_012952777.2	XP_012808231.1	ral GTPase-activating protein subunit beta isoform X2
XM_012952778.2	XP_012808232.1	ral GTPase-activating protein subunit beta isoform X3
XM_012952780.2	XP_012808234.1	ral GTPase-activating protein subunit beta isoform X5
XM_004918518.4	XP_004918575.2	ral GTPase-activating protein subunit beta isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa50226	XM_012952781.3 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa50227	XM_002932859.5 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa50228	XM_004918518.4 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa50222	XM_012952776.3 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa50223	XM_012952778.3 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa50224	XM_012952779.3 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa50225	XM_012952780.3 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa17271	XM_002932859.4 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa17272	XM_004918518.3 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa35913	XM_012952776.2 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa35914	XM_012952777.2 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa35915	XM_012952778.2 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa35916	XM_012952779.2 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa35917	XM_012952780.2 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa35918	XM_012952781.2 Latest version!	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ralgapb ( XM_012952781.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808235.2 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa50226
Clone ID Related Accession (Same CDS sequence)	XM_012952781.3
Accession Version	XM_012952781.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCATCTCGC GTCCCAGGGC CGACAGTGCT 
CCGCCGACCC CAGTGAACAG GCTAAGCATG CCTCAGAACG CCTCCACCAA CACTACGCCG 
CCGCACAGCC GAAGGCACCG CGCAGTCACT GTGAATAAGA CCACCGTAAA AACCAGCACG 
TCCGTCACAG CACATGGAGC AAAGGTGCAG CACCAACCGT CTTCCACCTC TCCCCTCTCC 
AGCCCCAACC AAACGAGCTC CGAGCCCCGA CCTCTCCCAG CCCCTATGAG GCCCAAAGTC 
AATAGCATCC TAAATCTGTT CGGTTCATGG CTGTTTGATG CAGCTTTGGT TCACTGCAGA 
CTGCAGAACG GAGTCAATCG AGACAGCAGC ATGACGGCAA TGACCACCCA GGCCGCCGTG 
GAGTTTCGGC GCAAAGGCTC CCAAATGTCT ACGGACACCA TGGTTTCCAG CACCGTGTTC 
GAAGCGGCAG ACTTCCCGGA GAATTACGAG GCAGGAAGGG CCGAGGCCTG CGGGACCCTG 
TGTAGGATAT TCTGTAGCAA GAAAACGGGA GAGGAAATCC TGCCTGCCTA TCTGTCCAGG 
TTTTATATGG TCTTAGTCCA AGGTCTTCAG ATTGGAGACT TTATCTGCCA CCCCGTGCTA 
GCAAGCATCA TCCTGAACTC TCCGCCCCTT TTCTGCTGTG ACCTCAAGGG GATCAACGTT 
GTGGTTCCTT ACTTCATCTC TGCACTTGAA ACCATTTTGC CAGACAGGGA ACTTTCAAAG 
TTTAAAAACT ATGTGAATCC CATAGAACTC AGAAGATCCT CCATTCACAT TTTACTTTCC 
TTGCTGCCTC TGCCCCACCA CTTTGGCACC CTCAGGTCGG AGGTCCTACT GGAAGGAAAG 
TTTAGCAACG ATGACAGCTT GGGCAGCGAC AAACCGGTCA CCTTTCTTTC CTTAAAGCTG 
CGACTCGTCA ATATACTGAT AGGAGCGCTG CAAACTGAAA CGGATCCCAA CAACACACAA 
ATGATTCTAG GTGCCATGTT AAACATTGTC CAAGATTCAG CATTTCTGGA GGCAACTGGC 
AACCAGGTAG AAATGATTGA AAATAACCTA ATGAAAAGCC ACAGTCGGAC GAACAGCGGT 
ATCAGCACAG CGAGTGGCAA CAGCACAGAG CCGACCACTC CGGACAGCGA GAGACCGGCG 
CAGGCCTTGC TGAGAGACTA CGGATACCCA GCTCTTAATA CAGATACAGC GGCCGGCCTG 
CTCATGCGCA GCATCCACTT GGTCACCCAG AGACTCAACT CGCAGTGGAG GCCGGACATG 
AGCATCTCGC TGGCTGCCTT GGAACTGCTG TCCGGACTGG CCAAGGTAAA GGTGGGTATA 
GACTCTTCAG ACAGGAAGAG GGCAGTGAGT TCTGTGTGCA CCTACATCGT ATACCAGTGC 
TCCCGCCCGG CTCCGCATCA CTCCCGGGAT CTGCACTCCA TGATTGTAGC TGCCTTCCAG 
TGCCTGTGTG TGTGGCTTAC AGAGCATCCA GACATGTTAA ATGAAAAGGA TTGTCTAAAA 
GAGGTTCTAG AGATTGTAGA ACTGGGCATA TCCGGTAGCA AGTCAAAAAA TCCTGAAATG 
CAAGAGGTGC GTTACAAGGG GGATAAGGAG CCCAACCCAG CCTCTATGCG GGTGAAGGAC 
GCTGCAGAAG CAACACTTAC ATGTATCATG CAGCTGCTTG GGGCTTTCCC TCCGCCCAGC 
GGCCCGGCCT CGCCCTGCAG CTTGGTAAAT GAGATGACGC TCATCACATA CTCTAGGCTG 
CCCACGGTCA GCAAATACAG CTTCCGATAC TTTGTGCTGG ACAACAGTGT TATACTGGCC 
ATGCTGGAGC AGCCCCTGGG CAACGAGCAA AGTAACCCCA GTCCATCAAT CACAGTTCTG 
ATCCGAGGGA TGTCTGGCCG CCATGCATGG TCTATGCAGC TGTGTCACCT TCCAAGAGCT 
GCAAGAGCCA ATCAGAGGAT GTTTATCCCC GAGCCGAGGC CGGAGCCTAA TAACTACGTA 
GGCATCAAAT ACAACGTAAA GCATCGTCCT TTCCCAGAGG AGGTGGATAA GATTCCCTTT 
GTTAAGGCCG ATCTGAGCAT CCCTGATTTA CACGAGATTG TCAATGAAGA GTTGGAAGAA 
AGGCACGAAA AGTTGAAGCA CGGGATGGCC AAACAGATAG CTTATGAGGC CGAGCTGGAC 
CACCTGAAAG AGGAGGAGCT TAAAAAGAGA AGTTATCCTG ACCCTGTAAC GGAGTGCAAG 
CCTCCCCATC CAGCCCAGGA GTTCCAGACT GCCCGCCTGC TCCTCTCCCA CTTTGGCTTC 
CTCTCTCTTG AAGCACTTAA AGAACCTGCA AACAGCCGCC TACCTCCTCA CCTCATTGCA 
CTTGACTCAC ACATGCCAGG ATTCTTTGAA GATATTGGGT ACTTGGACCT ATTGCCCTGC 
CGCCCCTTCG ATACTGTTTT TATCTTCTAC ATGAAGGCTG GACAAAAAAC AGGTCAAGAG 
ATCTTGAAGA ATGTAGAGTC TTCCAAAAAC GTTCAGCCAC ATTTCCTGGA GTTCCTGCTG 
TCCTTGGGCT GGCCCGTGGA TGTCGGGAAG CACCCAGGCT GGACAGGCCA TGTTTCTACC 
AGTTGGTCCA TCAACTGCAG TGGTGAGAAT GAGCAACACG AGCAAGAGGA AGCACCTTCG 
GTAGATGACA CAGGAGGGAG CATATTTAAC GGGGAAAAGA AAGTTCTCTA CTATGCGGAT 
GCCCTGACTG AGATCGCCTT TGTTGTTCCG TCAGCTGTGG ATGCTCAAAG CGACTCTGTG 
GAAAATAACA CCTCGGAGCT TGACGGGGAC TCAAACATGG ATCTTCTCCC AGGGATACTG 
AAGCAGCCGC TCACCCTTGA GCTTTTCCCT ACACACGTTG AAAATGTCAG CTCAGCACAA 
AGGCATAGTC CCACGTCGCG CACTAAGAAG CTGCCCCAGG GGCGTGTAGT CCCACCTATG 
GGTCCCGAGA CCAAGGTAGC AGTTGTATGG CTGGAGCAGT ACGATGATTT AGAAAACTTC 
CCTCTACCAG ACCTCGCAGC AGAGACCAGC ACTGGTATAG AGACCACTAC AAACAGCAGC 
ACTTCTATCC GCAACAGTCT GCCTGAGAAA GAGTTCCCTG TGATTTTCAT CCACCCTTTG 
TACACCGGCC TCTTCCGAGT TAAAGTGCAC GGAGCCTCTG GGAAATTCAA CATGGTGATC 
CCTCTGGTAG ATGGAATGGT GGTGAGCAGA AGAGCACTTG GTTTCTTGGT CAGACAGACC 
GTCATCAACA TCTGCCGGCG GAAGCGACTG GAGAGCGACT CGTACAACAC CCCGCACGTG 
CGTCGGAAGC AGAAAATCAC CGACATCGTC AATAAGTACC GGAACCGGCA GCTGGAGCCG 
GAGTTTTACA CCTCTCTCTT TCATGACGTC GGGCTGAAGA GCTGCACCCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808235.2
CDS	194..4687
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFEAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYGYPALNTDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_012952781.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952781.3

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCATCTCGC GTCCCAGGGC CGACAGTGCT 
CCGCCGACCC CAGTGAACAG GCTAAGCATG CCTCAGAACG CCTCCACCAA CACTACGCCG 
CCGCACAGCC GAAGGCACCG CGCAGTCACT GTGAATAAGA CCACCGTAAA AACCAGCACG 
TCCGTCACAG CACATGGAGC AAAGGTGCAG CACCAACCGT CTTCCACCTC TCCCCTCTCC 
AGCCCCAACC AAACGAGCTC CGAGCCCCGA CCTCTCCCAG CCCCTATGAG GCCCAAAGTC 
AATAGCATCC TAAATCTGTT CGGTTCATGG CTGTTTGATG CAGCTTTGGT TCACTGCAGA 
CTGCAGAACG GAGTCAATCG AGACAGCAGC ATGACGGCAA TGACCACCCA GGCCGCCGTG 
GAGTTTCGGC GCAAAGGCTC CCAAATGTCT ACGGACACCA TGGTTTCCAG CACCGTGTTC 
GAAGCGGCAG ACTTCCCGGA GAATTACGAG GCAGGAAGGG CCGAGGCCTG CGGGACCCTG 
TGTAGGATAT TCTGTAGCAA GAAAACGGGA GAGGAAATCC TGCCTGCCTA TCTGTCCAGG 
TTTTATATGG TCTTAGTCCA AGGTCTTCAG ATTGGAGACT TTATCTGCCA CCCCGTGCTA 
GCAAGCATCA TCCTGAACTC TCCGCCCCTT TTCTGCTGTG ACCTCAAGGG GATCAACGTT 
GTGGTTCCTT ACTTCATCTC TGCACTTGAA ACCATTTTGC CAGACAGGGA ACTTTCAAAG 
TTTAAAAACT ATGTGAATCC CATAGAACTC AGAAGATCCT CCATTCACAT TTTACTTTCC 
TTGCTGCCTC TGCCCCACCA CTTTGGCACC CTCAGGTCGG AGGTCCTACT GGAAGGAAAG 
TTTAGCAACG ATGACAGCTT GGGCAGCGAC AAACCGGTCA CCTTTCTTTC CTTAAAGCTG 
CGACTCGTCA ATATACTGAT AGGAGCGCTG CAAACTGAAA CGGATCCCAA CAACACACAA 
ATGATTCTAG GTGCCATGTT AAACATTGTC CAAGATTCAG CATTTCTGGA GGCAACTGGC 
AACCAGGTAG AAATGATTGA AAATAACCTA ATGAAAAGCC ACAGTCGGAC GAACAGCGGT 
ATCAGCACAG CGAGTGGCAA CAGCACAGAG CCGACCACTC CGGACAGCGA GAGACCGGCG 
CAGGCCTTGC TGAGAGACTA CGGATACCCA GCTCTTAATA CAGATACAGC GGCCGGCCTG 
CTCATGCGCA GCATCCACTT GGTCACCCAG AGACTCAACT CGCAGTGGAG GCCGGACATG 
AGCATCTCGC TGGCTGCCTT GGAACTGCTG TCCGGACTGG CCAAGGTAAA GGTGGGTATA 
GACTCTTCAG ACAGGAAGAG GGCAGTGAGT TCTGTGTGCA CCTACATCGT ATACCAGTGC 
TCCCGCCCGG CTCCGCATCA CTCCCGGGAT CTGCACTCCA TGATTGTAGC TGCCTTCCAG 
TGCCTGTGTG TGTGGCTTAC AGAGCATCCA GACATGTTAA ATGAAAAGGA TTGTCTAAAA 
GAGGTTCTAG AGATTGTAGA ACTGGGCATA TCCGGTAGCA AGTCAAAAAA TCCTGAAATG 
CAAGAGGTGC GTTACAAGGG GGATAAGGAG CCCAACCCAG CCTCTATGCG GGTGAAGGAC 
GCTGCAGAAG CAACACTTAC ATGTATCATG CAGCTGCTTG GGGCTTTCCC TCCGCCCAGC 
GGCCCGGCCT CGCCCTGCAG CTTGGTAAAT GAGATGACGC TCATCACATA CTCTAGGCTG 
CCCACGGTCA GCAAATACAG CTTCCGATAC TTTGTGCTGG ACAACAGTGT TATACTGGCC 
ATGCTGGAGC AGCCCCTGGG CAACGAGCAA AGTAACCCCA GTCCATCAAT CACAGTTCTG 
ATCCGAGGGA TGTCTGGCCG CCATGCATGG TCTATGCAGC TGTGTCACCT TCCAAGAGCT 
GCAAGAGCCA ATCAGAGGAT GTTTATCCCC GAGCCGAGGC CGGAGCCTAA TAACTACGTA 
GGCATCAAAT ACAACGTAAA GCATCGTCCT TTCCCAGAGG AGGTGGATAA GATTCCCTTT 
GTTAAGGCCG ATCTGAGCAT CCCTGATTTA CACGAGATTG TCAATGAAGA GTTGGAAGAA 
AGGCACGAAA AGTTGAAGCA CGGGATGGCC AAACAGATAG CTTATGAGGC CGAGCTGGAC 
CACCTGAAAG AGGAGGAGCT TAAAAAGAGA AGTTATCCTG ACCCTGTAAC GGAGTGCAAG 
CCTCCCCATC CAGCCCAGGA GTTCCAGACT GCCCGCCTGC TCCTCTCCCA CTTTGGCTTC 
CTCTCTCTTG AAGCACTTAA AGAACCTGCA AACAGCCGCC TACCTCCTCA CCTCATTGCA 
CTTGACTCAC ACATGCCAGG ATTCTTTGAA GATATTGGGT ACTTGGACCT ATTGCCCTGC 
CGCCCCTTCG ATACTGTTTT TATCTTCTAC ATGAAGGCTG GACAAAAAAC AGGTCAAGAG 
ATCTTGAAGA ATGTAGAGTC TTCCAAAAAC GTTCAGCCAC ATTTCCTGGA GTTCCTGCTG 
TCCTTGGGCT GGCCCGTGGA TGTCGGGAAG CACCCAGGCT GGACAGGCCA TGTTTCTACC 
AGTTGGTCCA TCAACTGCAG TGGTGAGAAT GAGCAACACG AGCAAGAGGA AGCACCTTCG 
GTAGATGACA CAGGAGGGAG CATATTTAAC GGGGAAAAGA AAGTTCTCTA CTATGCGGAT 
GCCCTGACTG AGATCGCCTT TGTTGTTCCG TCAGCTGTGG ATGCTCAAAG CGACTCTGTG 
GAAAATAACA CCTCGGAGCT TGACGGGGAC TCAAACATGG ATCTTCTCCC AGGGATACTG 
AAGCAGCCGC TCACCCTTGA GCTTTTCCCT ACACACGTTG AAAATGTCAG CTCAGCACAA 
AGGCATAGTC CCACGTCGCG CACTAAGAAG CTGCCCCAGG GGCGTGTAGT CCCACCTATG 
GGTCCCGAGA CCAAGGTAGC AGTTGTATGG CTGGAGCAGT ACGATGATTT AGAAAACTTC 
CCTCTACCAG ACCTCGCAGC AGAGACCAGC ACTGGTATAG AGACCACTAC AAACAGCAGC 
ACTTCTATCC GCAACAGTCT GCCTGAGAAA GAGTTCCCTG TGATTTTCAT CCACCCTTTG 
TACACCGGCC TCTTCCGAGT TAAAGTGCAC GGAGCCTCTG GGAAATTCAA CATGGTGATC 
CCTCTGGTAG ATGGAATGGT GGTGAGCAGA AGAGCACTTG GTTTCTTGGT CAGACAGACC 
GTCATCAACA TCTGCCGGCG GAAGCGACTG GAGAGCGACT CGTACAACAC CCCGCACGTG 
CGTCGGAAGC AGAAAATCAC CGACATCGTC AATAAGTACC GGAACCGGCA GCTGGAGCCG 
GAGTTTTACA CCTCTCTCTT TCATGACGTC GGGCTGAAGA GCTGCACCCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_002932859.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932905.2 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa50227
Clone ID Related Accession (Same CDS sequence)	XM_002932859.5
Accession Version	XM_002932859.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932859.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCATCTCGC GTCCCAGGGC CGACAGTGCT 
CCGCCGACCC CAGTGAACAG GCTAAGCATG CCTCAGAACG CCTCCACCAA CACTACGCCG 
CCGCACAGCC GAAGGCACCG CGCAGTCACT GTGAATAAGA CCACCGTAAA AACCAGCACG 
TCCGTCACAG CACATGGAGC AAAGGTGCAG CACCAACCGT CTTCCACCTC TCCCCTCTCC 
AGCCCCAACC AAACGAGCTC CGAGCCCCGA CCTCTCCCAG CCCCTATGAG GCCCAAAGTC 
AATAGCATCC TAAATCTGTT CGGTTCATGG CTGTTTGATG CAGCTTTGGT TCACTGCAGA 
CTGCAGAACG GAGTCAATCG AGACAGCAGC ATGACGGCAA TGACCACCCA GGCCGCCGTG 
GAGTTTCGGC GCAAAGGCTC CCAAATGTCT ACGGACACCA TGGTTTCCAG CACCGTGTTC 
GAAGCGGCAG ACTTCCCGGA GAATTACGAG GCAGGAAGGG CCGAGGCCTG CGGGACCCTG 
TGTAGGATAT TCTGTAGCAA GAAAACGGGA GAGGAAATCC TGCCTGCCTA TCTGTCCAGG 
TTTTATATGG TCTTAGTCCA AGGTCTTCAG ATTGGAGACT TTATCTGCCA CCCCGTGCTA 
GCAAGCATCA TCCTGAACTC TCCGCCCCTT TTCTGCTGTG ACCTCAAGGG GATCAACGTT 
GTGGTTCCTT ACTTCATCTC TGCACTTGAA ACCATTTTGC CAGACAGGGA ACTTTCAAAG 
TTTAAAAACT ATGTGAATCC CATAGAACTC AGAAGATCCT CCATTCACAT TTTACTTTCC 
TTGCTGCCTC TGCCCCACCA CTTTGGCACC CTCAGGTCGG AGGTCCTACT GGAAGGAAAG 
TTTAGCAACG ATGACAGCTT GGGCAGCGAC AAACCGGTCA CCTTTCTTTC CTTAAAGCTG 
CGACTCGTCA ATATACTGAT AGGAGCGCTG CAAACTGAAA CGGATCCCAA CAACACACAA 
ATGATTCTAG GTGCCATGTT AAACATTGTC CAAGATTCAG CATTTCTGGA GGCAACTGGC 
AACCAGGTAG AAATGATTGA AAATAACCTA ATGAAAAGCC ACAGTCGGAC GAACAGCGGT 
ATCAGCACAG CGAGTGGCAA CAGCACAGAG CCGACCACTC CGGACAGCGA GAGACCGGCG 
CAGGCCTTGC TGAGAGACTA CGCTCTTAAT ACAGATACAG CGGCCGGCCT GCTCATGCGC 
AGCATCCACT TGGTCACCCA GAGACTCAAC TCGCAGTGGA GGCCGGACAT GAGCATCTCG 
CTGGCTGCCT TGGAACTGCT GTCCGGACTG GCCAAGGTAA AGGTGGGTAT AGACTCTTCA 
GACAGGAAGA GGGCAGTGAG TTCTGTGTGC ACCTACATCG TATACCAGTG CTCCCGCCCG 
GCTCCGCATC ACTCCCGGGA TCTGCACTCC ATGATTGTAG CTGCCTTCCA GTGCCTGTGT 
GTGTGGCTTA CAGAGCATCC AGACATGTTA AATGAAAAGG ATTGTCTAAA AGAGGTTCTA 
GAGATTGTAG AACTGGGCAT ATCCGGTAGC AAGTCAAAAA ATCCTGAAAT GCAAGAGGTG 
CGTTACAAGG GGGATAAGGA GCCCAACCCA GCCTCTATGC GGGTGAAGGA CGCTGCAGAA 
GCAACACTTA CATGTATCAT GCAGCTGCTT GGGGCTTTCC CTCCGCCCAG CGGCCCGGCC 
TCGCCCTGCA GCTTGGTAAA TGAGATGACG CTCATCACAT ACTCTAGGCT GCCCACGGTC 
AGCAAATACA GCTTCCGATA CTTTGTGCTG GACAACAGTG TTATACTGGC CATGCTGGAG 
CAGCCCCTGG GCAACGAGCA AAGTAACCCC AGTCCATCAA TCACAGTTCT GATCCGAGGG 
ATGTCTGGCC GCCATGCATG GTCTATGCAG CTGTGTCACC TTCCAAGAGC TGCAAGAGCC 
AATCAGAGGA TGTTTATCCC CGAGCCGAGG CCGGAGCCTA ATAACTACGT AGGCATCAAA 
TACAACGTAA AGCATCGTCC TTTCCCAGAG GAGGTGGATA AGATTCCCTT TGTTAAGGCC 
GATCTGAGCA TCCCTGATTT ACACGAGATT GTCAATGAAG AGTTGGAAGA AAGGCACGAA 
AAGTTGAAGC ACGGGATGGC CAAACAGATA GCTTATGAGG CCGAGCTGGA CCACCTGAAA 
GAGGAGGAGC TTAAAAAGAG AAGTTATCCT GACCCTGTAA CGGAGTGCAA GCCTCCCCAT 
CCAGCCCAGG AGTTCCAGAC TGCCCGCCTG CTCCTCTCCC ACTTTGGCTT CCTCTCTCTT 
GAAGCACTTA AAGAACCTGC AAACAGCCGC CTACCTCCTC ACCTCATTGC ACTTGACTCA 
CACATGCCAG GATTCTTTGA AGATATTGGG TACTTGGACC TATTGCCCTG CCGCCCCTTC 
GATACTGTTT TTATCTTCTA CATGAAGGCT GGACAAAAAA CAGGTCAAGA GATCTTGAAG 
AATGTAGAGT CTTCCAAAAA CGTTCAGCCA CATTTCCTGG AGTTCCTGCT GTCCTTGGGC 
TGGCCCGTGG ATGTCGGGAA GCACCCAGGC TGGACAGGCC ATGTTTCTAC CAGTTGGTCC 
ATCAACTGCA GTGGTGAGAA TGAGCAACAC GAGCAAGAGG AAGCACCTTC GGTAGATGAC 
ACAGGAGGGA GCATATTTAA CGGGGAAAAG AAAGTTCTCT ACTATGCGGA TGCCCTGACT 
GAGATCGCCT TTGTTGTTCC GTCAGCTGTG GATGCTCAAA GCGACTCTGT GGAAAATAAC 
ACCTCGGAGC TTGACGGGGA CTCAAACATG GATCTTCTCC CAGGGATACT GAAGCAGCCG 
CTCACCCTTG AGCTTTTCCC TACACACGTT GAAAATGTCA GCTCAGCACA AAGGCATAGT 
CCCACGTCGC GCACTAAGAA GCTGCCCCAG GGGCGTGTAG TCCCACCTAT GGGTCCCGAG 
ACCAAGGTAG CAGTTGTATG GCTGGAGCAG TACGATGATT TAGAAAACTT CCCTCTACCA 
GACCTCGCAG CAGAGACCAG CACTGGTATA GAGACCACTA CAAACAGCAG CACTTCTATC 
CGCAACAGTC TGCCTGAGAA AGAGTTCCCT GTGATTTTCA TCCACCCTTT GTACACCGGC 
CTCTTCCGAG TTAAAGTGCA CGGAGCCTCT GGGAAATTCA ACATGGTGAT CCCTCTGGTA 
GATGGAATGG TGGTGAGCAG AAGAGCACTT GGTTTCTTGG TCAGACAGAC CGTCATCAAC 
ATCTGCCGGC GGAAGCGACT GGAGAGCGAC TCGTACAACA CCCCGCACGT GCGTCGGAAG 
CAGAAAATCA CCGACATCGT CAATAAGTAC CGGAACCGGC AGCTGGAGCC GGAGTTTTAC 
ACCTCTCTCT TTCATGACGT CGGGCTGAAG AGCTGCACCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932905.2
CDS	194..4678
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFEAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYALNTDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_002932859.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932859.5

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCATCTCGC GTCCCAGGGC CGACAGTGCT 
CCGCCGACCC CAGTGAACAG GCTAAGCATG CCTCAGAACG CCTCCACCAA CACTACGCCG 
CCGCACAGCC GAAGGCACCG CGCAGTCACT GTGAATAAGA CCACCGTAAA AACCAGCACG 
TCCGTCACAG CACATGGAGC AAAGGTGCAG CACCAACCGT CTTCCACCTC TCCCCTCTCC 
AGCCCCAACC AAACGAGCTC CGAGCCCCGA CCTCTCCCAG CCCCTATGAG GCCCAAAGTC 
AATAGCATCC TAAATCTGTT CGGTTCATGG CTGTTTGATG CAGCTTTGGT TCACTGCAGA 
CTGCAGAACG GAGTCAATCG AGACAGCAGC ATGACGGCAA TGACCACCCA GGCCGCCGTG 
GAGTTTCGGC GCAAAGGCTC CCAAATGTCT ACGGACACCA TGGTTTCCAG CACCGTGTTC 
GAAGCGGCAG ACTTCCCGGA GAATTACGAG GCAGGAAGGG CCGAGGCCTG CGGGACCCTG 
TGTAGGATAT TCTGTAGCAA GAAAACGGGA GAGGAAATCC TGCCTGCCTA TCTGTCCAGG 
TTTTATATGG TCTTAGTCCA AGGTCTTCAG ATTGGAGACT TTATCTGCCA CCCCGTGCTA 
GCAAGCATCA TCCTGAACTC TCCGCCCCTT TTCTGCTGTG ACCTCAAGGG GATCAACGTT 
GTGGTTCCTT ACTTCATCTC TGCACTTGAA ACCATTTTGC CAGACAGGGA ACTTTCAAAG 
TTTAAAAACT ATGTGAATCC CATAGAACTC AGAAGATCCT CCATTCACAT TTTACTTTCC 
TTGCTGCCTC TGCCCCACCA CTTTGGCACC CTCAGGTCGG AGGTCCTACT GGAAGGAAAG 
TTTAGCAACG ATGACAGCTT GGGCAGCGAC AAACCGGTCA CCTTTCTTTC CTTAAAGCTG 
CGACTCGTCA ATATACTGAT AGGAGCGCTG CAAACTGAAA CGGATCCCAA CAACACACAA 
ATGATTCTAG GTGCCATGTT AAACATTGTC CAAGATTCAG CATTTCTGGA GGCAACTGGC 
AACCAGGTAG AAATGATTGA AAATAACCTA ATGAAAAGCC ACAGTCGGAC GAACAGCGGT 
ATCAGCACAG CGAGTGGCAA CAGCACAGAG CCGACCACTC CGGACAGCGA GAGACCGGCG 
CAGGCCTTGC TGAGAGACTA CGCTCTTAAT ACAGATACAG CGGCCGGCCT GCTCATGCGC 
AGCATCCACT TGGTCACCCA GAGACTCAAC TCGCAGTGGA GGCCGGACAT GAGCATCTCG 
CTGGCTGCCT TGGAACTGCT GTCCGGACTG GCCAAGGTAA AGGTGGGTAT AGACTCTTCA 
GACAGGAAGA GGGCAGTGAG TTCTGTGTGC ACCTACATCG TATACCAGTG CTCCCGCCCG 
GCTCCGCATC ACTCCCGGGA TCTGCACTCC ATGATTGTAG CTGCCTTCCA GTGCCTGTGT 
GTGTGGCTTA CAGAGCATCC AGACATGTTA AATGAAAAGG ATTGTCTAAA AGAGGTTCTA 
GAGATTGTAG AACTGGGCAT ATCCGGTAGC AAGTCAAAAA ATCCTGAAAT GCAAGAGGTG 
CGTTACAAGG GGGATAAGGA GCCCAACCCA GCCTCTATGC GGGTGAAGGA CGCTGCAGAA 
GCAACACTTA CATGTATCAT GCAGCTGCTT GGGGCTTTCC CTCCGCCCAG CGGCCCGGCC 
TCGCCCTGCA GCTTGGTAAA TGAGATGACG CTCATCACAT ACTCTAGGCT GCCCACGGTC 
AGCAAATACA GCTTCCGATA CTTTGTGCTG GACAACAGTG TTATACTGGC CATGCTGGAG 
CAGCCCCTGG GCAACGAGCA AAGTAACCCC AGTCCATCAA TCACAGTTCT GATCCGAGGG 
ATGTCTGGCC GCCATGCATG GTCTATGCAG CTGTGTCACC TTCCAAGAGC TGCAAGAGCC 
AATCAGAGGA TGTTTATCCC CGAGCCGAGG CCGGAGCCTA ATAACTACGT AGGCATCAAA 
TACAACGTAA AGCATCGTCC TTTCCCAGAG GAGGTGGATA AGATTCCCTT TGTTAAGGCC 
GATCTGAGCA TCCCTGATTT ACACGAGATT GTCAATGAAG AGTTGGAAGA AAGGCACGAA 
AAGTTGAAGC ACGGGATGGC CAAACAGATA GCTTATGAGG CCGAGCTGGA CCACCTGAAA 
GAGGAGGAGC TTAAAAAGAG AAGTTATCCT GACCCTGTAA CGGAGTGCAA GCCTCCCCAT 
CCAGCCCAGG AGTTCCAGAC TGCCCGCCTG CTCCTCTCCC ACTTTGGCTT CCTCTCTCTT 
GAAGCACTTA AAGAACCTGC AAACAGCCGC CTACCTCCTC ACCTCATTGC ACTTGACTCA 
CACATGCCAG GATTCTTTGA AGATATTGGG TACTTGGACC TATTGCCCTG CCGCCCCTTC 
GATACTGTTT TTATCTTCTA CATGAAGGCT GGACAAAAAA CAGGTCAAGA GATCTTGAAG 
AATGTAGAGT CTTCCAAAAA CGTTCAGCCA CATTTCCTGG AGTTCCTGCT GTCCTTGGGC 
TGGCCCGTGG ATGTCGGGAA GCACCCAGGC TGGACAGGCC ATGTTTCTAC CAGTTGGTCC 
ATCAACTGCA GTGGTGAGAA TGAGCAACAC GAGCAAGAGG AAGCACCTTC GGTAGATGAC 
ACAGGAGGGA GCATATTTAA CGGGGAAAAG AAAGTTCTCT ACTATGCGGA TGCCCTGACT 
GAGATCGCCT TTGTTGTTCC GTCAGCTGTG GATGCTCAAA GCGACTCTGT GGAAAATAAC 
ACCTCGGAGC TTGACGGGGA CTCAAACATG GATCTTCTCC CAGGGATACT GAAGCAGCCG 
CTCACCCTTG AGCTTTTCCC TACACACGTT GAAAATGTCA GCTCAGCACA AAGGCATAGT 
CCCACGTCGC GCACTAAGAA GCTGCCCCAG GGGCGTGTAG TCCCACCTAT GGGTCCCGAG 
ACCAAGGTAG CAGTTGTATG GCTGGAGCAG TACGATGATT TAGAAAACTT CCCTCTACCA 
GACCTCGCAG CAGAGACCAG CACTGGTATA GAGACCACTA CAAACAGCAG CACTTCTATC 
CGCAACAGTC TGCCTGAGAA AGAGTTCCCT GTGATTTTCA TCCACCCTTT GTACACCGGC 
CTCTTCCGAG TTAAAGTGCA CGGAGCCTCT GGGAAATTCA ACATGGTGAT CCCTCTGGTA 
GATGGAATGG TGGTGAGCAG AAGAGCACTT GGTTTCTTGG TCAGACAGAC CGTCATCAAC 
ATCTGCCGGC GGAAGCGACT GGAGAGCGAC TCGTACAACA CCCCGCACGT GCGTCGGAAG 
CAGAAAATCA CCGACATCGT CAATAAGTAC CGGAACCGGC AGCTGGAGCC GGAGTTTTAC 
ACCTCTCTCT TTCATGACGT CGGGCTGAAG AGCTGCACCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_004918518.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918575.2 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X7, mRNA.

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CloneID	OXa50228
Clone ID Related Accession (Same CDS sequence)	XM_004918518.4
Accession Version	XM_004918518.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004918518.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCATCTCGC GTCCCAGGGC CGACAGTGCT 
CCGCCGACCC CAGTGAACAG GCTAAGCATG CCTCAGAACG CCTCCACCAA CACTACGCCG 
CCGCACAGCC GAAGGCACCG CGCAGTCACT GTGAATAAGA CCACCGTAAA AACCAGCACG 
TCCGTCACAG CACATGGAGC AAAGGTGCAG CACCAACCGT CTTCCACCTC TCCCCTCTCC 
AGCCCCAACC AAACGAGCTC CGAGCCCCGA CCTCTCCCAG CCCCTATGAG GCCCAAAGTC 
AATAGCATCC TAAATCTGTT CGGTTCATGG CTGTTTGATG CAGCTTTGGT TCACTGCAGA 
CTGCAGAACG GAGTCAATCG AGACAGCAGC ATGACGGCAA TGACCACCCA GGCCGCCGTG 
GAGTTTCGGC GCAAAGGCTC CCAAATGTCT ACGGACACCA TGGTTTCCAG CACCGTGTTC 
GAAGCGGCAG ACTTCCCGGA GAATTACGAG GCAGGAAGGG CCGAGGCCTG CGGGACCCTG 
TGTAGGATAT TCTGTAGCAA GAAAACGGGA GAGGAAATCC TGCCTGCCTA TCTGTCCAGG 
TTTTATATGG TCTTAGTCCA AGGTCTTCAG ATTGGAGACT TTATCTGCCA CCCCGTGCTA 
GCAAGCATCA TCCTGAACTC TCCGCCCCTT TTCTGCTGTG ACCTCAAGGG GATCAACGTT 
GTGGTTCCTT ACTTCATCTC TGCACTTGAA ACCATTTTGC CAGACAGGGA ACTTTCAAAG 
TTTAAAAACT ATGTGAATCC CATAGAACTC AGAAGATCCT CCATTCACAT TTTACTTTCC 
TTGCTGCCTC TGCCCCACCA CTTTGGCACC CTCAGGTCGG AGGTCCTACT GGAAGGAAAG 
TTTAGCAACG ATGACAGCTT GGGCAGCGAC AAACCGGTCA CCTTTCTTTC CTTAAAGCTG 
CGACTCGTCA ATATACTGAT AGGAGCGCTG CAAACTGAAA CGGATCCCAA CAACACACAA 
ATGATTCTAG GTGCCATGTT AAACATTGTC CAAGATTCAG CATTTCTGGA GGCAACTGGC 
AACCAGGTAG AAATGATTGA AAATAACCTA ATGAAAAGCC ACAGTCGGAC GAACAGCGGT 
ATCAGCACAG CGAGTGGCAA CAGCACAGAG CCGACCACTC CGGACAGCGA GAGACCGGCG 
CAGGCCTTGC TGAGAGACTA CGATACAGCG GCCGGCCTGC TCATGCGCAG CATCCACTTG 
GTCACCCAGA GACTCAACTC GCAGTGGAGG CCGGACATGA GCATCTCGCT GGCTGCCTTG 
GAACTGCTGT CCGGACTGGC CAAGGTAAAG GTGGGTATAG ACTCTTCAGA CAGGAAGAGG 
GCAGTGAGTT CTGTGTGCAC CTACATCGTA TACCAGTGCT CCCGCCCGGC TCCGCATCAC 
TCCCGGGATC TGCACTCCAT GATTGTAGCT GCCTTCCAGT GCCTGTGTGT GTGGCTTACA 
GAGCATCCAG ACATGTTAAA TGAAAAGGAT TGTCTAAAAG AGGTTCTAGA GATTGTAGAA 
CTGGGCATAT CCGGTAGCAA GTCAAAAAAT CCTGAAATGC AAGAGGTGCG TTACAAGGGG 
GATAAGGAGC CCAACCCAGC CTCTATGCGG GTGAAGGACG CTGCAGAAGC AACACTTACA 
TGTATCATGC AGCTGCTTGG GGCTTTCCCT CCGCCCAGCG GCCCGGCCTC GCCCTGCAGC 
TTGGTAAATG AGATGACGCT CATCACATAC TCTAGGCTGC CCACGGTCAG CAAATACAGC 
TTCCGATACT TTGTGCTGGA CAACAGTGTT ATACTGGCCA TGCTGGAGCA GCCCCTGGGC 
AACGAGCAAA GTAACCCCAG TCCATCAATC ACAGTTCTGA TCCGAGGGAT GTCTGGCCGC 
CATGCATGGT CTATGCAGCT GTGTCACCTT CCAAGAGCTG CAAGAGCCAA TCAGAGGATG 
TTTATCCCCG AGCCGAGGCC GGAGCCTAAT AACTACGTAG GCATCAAATA CAACGTAAAG 
CATCGTCCTT TCCCAGAGGA GGTGGATAAG ATTCCCTTTG TTAAGGCCGA TCTGAGCATC 
CCTGATTTAC ACGAGATTGT CAATGAAGAG TTGGAAGAAA GGCACGAAAA GTTGAAGCAC 
GGGATGGCCA AACAGATAGC TTATGAGGCC GAGCTGGACC ACCTGAAAGA GGAGGAGCTT 
AAAAAGAGAA GTTATCCTGA CCCTGTAACG GAGTGCAAGC CTCCCCATCC AGCCCAGGAG 
TTCCAGACTG CCCGCCTGCT CCTCTCCCAC TTTGGCTTCC TCTCTCTTGA AGCACTTAAA 
GAACCTGCAA ACAGCCGCCT ACCTCCTCAC CTCATTGCAC TTGACTCACA CATGCCAGGA 
TTCTTTGAAG ATATTGGGTA CTTGGACCTA TTGCCCTGCC GCCCCTTCGA TACTGTTTTT 
ATCTTCTACA TGAAGGCTGG ACAAAAAACA GGTCAAGAGA TCTTGAAGAA TGTAGAGTCT 
TCCAAAAACG TTCAGCCACA TTTCCTGGAG TTCCTGCTGT CCTTGGGCTG GCCCGTGGAT 
GTCGGGAAGC ACCCAGGCTG GACAGGCCAT GTTTCTACCA GTTGGTCCAT CAACTGCAGT 
GGTGAGAATG AGCAACACGA GCAAGAGGAA GCACCTTCGG TAGATGACAC AGGAGGGAGC 
ATATTTAACG GGGAAAAGAA AGTTCTCTAC TATGCGGATG CCCTGACTGA GATCGCCTTT 
GTTGTTCCGT CAGCTGTGGA TGCTCAAAGC GACTCTGTGG AAAATAACAC CTCGGAGCTT 
GACGGGGACT CAAACATGGA TCTTCTCCCA GGGATACTGA AGCAGCCGCT CACCCTTGAG 
CTTTTCCCTA CACACGTTGA AAATGTCAGC TCAGCACAAA GGCATAGTCC CACGTCGCGC 
ACTAAGAAGC TGCCCCAGGG GCGTGTAGTC CCACCTATGG GTCCCGAGAC CAAGGTAGCA 
GTTGTATGGC TGGAGCAGTA CGATGATTTA GAAAACTTCC CTCTACCAGA CCTCGCAGCA 
GAGACCAGCA CTGGTATAGA GACCACTACA AACAGCAGCA CTTCTATCCG CAACAGTCTG 
CCTGAGAAAG AGTTCCCTGT GATTTTCATC CACCCTTTGT ACACCGGCCT CTTCCGAGTT 
AAAGTGCACG GAGCCTCTGG GAAATTCAAC ATGGTGATCC CTCTGGTAGA TGGAATGGTG 
GTGAGCAGAA GAGCACTTGG TTTCTTGGTC AGACAGACCG TCATCAACAT CTGCCGGCGG 
AAGCGACTGG AGAGCGACTC GTACAACACC CCGCACGTGC GTCGGAAGCA GAAAATCACC 
GACATCGTCA ATAAGTACCG GAACCGGCAG CTGGAGCCGG AGTTTTACAC CTCTCTCTTT 
CATGACGTCG GGCTGAAGAG CTGCACCCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918575.2
CDS	194..4666
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFEAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_004918518.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918518.4

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCATCTCGC GTCCCAGGGC CGACAGTGCT 
CCGCCGACCC CAGTGAACAG GCTAAGCATG CCTCAGAACG CCTCCACCAA CACTACGCCG 
CCGCACAGCC GAAGGCACCG CGCAGTCACT GTGAATAAGA CCACCGTAAA AACCAGCACG 
TCCGTCACAG CACATGGAGC AAAGGTGCAG CACCAACCGT CTTCCACCTC TCCCCTCTCC 
AGCCCCAACC AAACGAGCTC CGAGCCCCGA CCTCTCCCAG CCCCTATGAG GCCCAAAGTC 
AATAGCATCC TAAATCTGTT CGGTTCATGG CTGTTTGATG CAGCTTTGGT TCACTGCAGA 
CTGCAGAACG GAGTCAATCG AGACAGCAGC ATGACGGCAA TGACCACCCA GGCCGCCGTG 
GAGTTTCGGC GCAAAGGCTC CCAAATGTCT ACGGACACCA TGGTTTCCAG CACCGTGTTC 
GAAGCGGCAG ACTTCCCGGA GAATTACGAG GCAGGAAGGG CCGAGGCCTG CGGGACCCTG 
TGTAGGATAT TCTGTAGCAA GAAAACGGGA GAGGAAATCC TGCCTGCCTA TCTGTCCAGG 
TTTTATATGG TCTTAGTCCA AGGTCTTCAG ATTGGAGACT TTATCTGCCA CCCCGTGCTA 
GCAAGCATCA TCCTGAACTC TCCGCCCCTT TTCTGCTGTG ACCTCAAGGG GATCAACGTT 
GTGGTTCCTT ACTTCATCTC TGCACTTGAA ACCATTTTGC CAGACAGGGA ACTTTCAAAG 
TTTAAAAACT ATGTGAATCC CATAGAACTC AGAAGATCCT CCATTCACAT TTTACTTTCC 
TTGCTGCCTC TGCCCCACCA CTTTGGCACC CTCAGGTCGG AGGTCCTACT GGAAGGAAAG 
TTTAGCAACG ATGACAGCTT GGGCAGCGAC AAACCGGTCA CCTTTCTTTC CTTAAAGCTG 
CGACTCGTCA ATATACTGAT AGGAGCGCTG CAAACTGAAA CGGATCCCAA CAACACACAA 
ATGATTCTAG GTGCCATGTT AAACATTGTC CAAGATTCAG CATTTCTGGA GGCAACTGGC 
AACCAGGTAG AAATGATTGA AAATAACCTA ATGAAAAGCC ACAGTCGGAC GAACAGCGGT 
ATCAGCACAG CGAGTGGCAA CAGCACAGAG CCGACCACTC CGGACAGCGA GAGACCGGCG 
CAGGCCTTGC TGAGAGACTA CGATACAGCG GCCGGCCTGC TCATGCGCAG CATCCACTTG 
GTCACCCAGA GACTCAACTC GCAGTGGAGG CCGGACATGA GCATCTCGCT GGCTGCCTTG 
GAACTGCTGT CCGGACTGGC CAAGGTAAAG GTGGGTATAG ACTCTTCAGA CAGGAAGAGG 
GCAGTGAGTT CTGTGTGCAC CTACATCGTA TACCAGTGCT CCCGCCCGGC TCCGCATCAC 
TCCCGGGATC TGCACTCCAT GATTGTAGCT GCCTTCCAGT GCCTGTGTGT GTGGCTTACA 
GAGCATCCAG ACATGTTAAA TGAAAAGGAT TGTCTAAAAG AGGTTCTAGA GATTGTAGAA 
CTGGGCATAT CCGGTAGCAA GTCAAAAAAT CCTGAAATGC AAGAGGTGCG TTACAAGGGG 
GATAAGGAGC CCAACCCAGC CTCTATGCGG GTGAAGGACG CTGCAGAAGC AACACTTACA 
TGTATCATGC AGCTGCTTGG GGCTTTCCCT CCGCCCAGCG GCCCGGCCTC GCCCTGCAGC 
TTGGTAAATG AGATGACGCT CATCACATAC TCTAGGCTGC CCACGGTCAG CAAATACAGC 
TTCCGATACT TTGTGCTGGA CAACAGTGTT ATACTGGCCA TGCTGGAGCA GCCCCTGGGC 
AACGAGCAAA GTAACCCCAG TCCATCAATC ACAGTTCTGA TCCGAGGGAT GTCTGGCCGC 
CATGCATGGT CTATGCAGCT GTGTCACCTT CCAAGAGCTG CAAGAGCCAA TCAGAGGATG 
TTTATCCCCG AGCCGAGGCC GGAGCCTAAT AACTACGTAG GCATCAAATA CAACGTAAAG 
CATCGTCCTT TCCCAGAGGA GGTGGATAAG ATTCCCTTTG TTAAGGCCGA TCTGAGCATC 
CCTGATTTAC ACGAGATTGT CAATGAAGAG TTGGAAGAAA GGCACGAAAA GTTGAAGCAC 
GGGATGGCCA AACAGATAGC TTATGAGGCC GAGCTGGACC ACCTGAAAGA GGAGGAGCTT 
AAAAAGAGAA GTTATCCTGA CCCTGTAACG GAGTGCAAGC CTCCCCATCC AGCCCAGGAG 
TTCCAGACTG CCCGCCTGCT CCTCTCCCAC TTTGGCTTCC TCTCTCTTGA AGCACTTAAA 
GAACCTGCAA ACAGCCGCCT ACCTCCTCAC CTCATTGCAC TTGACTCACA CATGCCAGGA 
TTCTTTGAAG ATATTGGGTA CTTGGACCTA TTGCCCTGCC GCCCCTTCGA TACTGTTTTT 
ATCTTCTACA TGAAGGCTGG ACAAAAAACA GGTCAAGAGA TCTTGAAGAA TGTAGAGTCT 
TCCAAAAACG TTCAGCCACA TTTCCTGGAG TTCCTGCTGT CCTTGGGCTG GCCCGTGGAT 
GTCGGGAAGC ACCCAGGCTG GACAGGCCAT GTTTCTACCA GTTGGTCCAT CAACTGCAGT 
GGTGAGAATG AGCAACACGA GCAAGAGGAA GCACCTTCGG TAGATGACAC AGGAGGGAGC 
ATATTTAACG GGGAAAAGAA AGTTCTCTAC TATGCGGATG CCCTGACTGA GATCGCCTTT 
GTTGTTCCGT CAGCTGTGGA TGCTCAAAGC GACTCTGTGG AAAATAACAC CTCGGAGCTT 
GACGGGGACT CAAACATGGA TCTTCTCCCA GGGATACTGA AGCAGCCGCT CACCCTTGAG 
CTTTTCCCTA CACACGTTGA AAATGTCAGC TCAGCACAAA GGCATAGTCC CACGTCGCGC 
ACTAAGAAGC TGCCCCAGGG GCGTGTAGTC CCACCTATGG GTCCCGAGAC CAAGGTAGCA 
GTTGTATGGC TGGAGCAGTA CGATGATTTA GAAAACTTCC CTCTACCAGA CCTCGCAGCA 
GAGACCAGCA CTGGTATAGA GACCACTACA AACAGCAGCA CTTCTATCCG CAACAGTCTG 
CCTGAGAAAG AGTTCCCTGT GATTTTCATC CACCCTTTGT ACACCGGCCT CTTCCGAGTT 
AAAGTGCACG GAGCCTCTGG GAAATTCAAC ATGGTGATCC CTCTGGTAGA TGGAATGGTG 
GTGAGCAGAA GAGCACTTGG TTTCTTGGTC AGACAGACCG TCATCAACAT CTGCCGGCGG 
AAGCGACTGG AGAGCGACTC GTACAACACC CCGCACGTGC GTCGGAAGCA GAAAATCACC 
GACATCGTCA ATAAGTACCG GAACCGGCAG CTGGAGCCGG AGTTTTACAC CTCTCTCTTT 
CATGACGTCG GGCTGAAGAG CTGCACCCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_012952776.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808230.2 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X1, mRNA.

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CloneID	OXa50222
Clone ID Related Accession (Same CDS sequence)	XM_012952776.3
Accession Version	XM_012952776.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952776.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA AGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCT TAAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCG 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG GATACCCAGC TCTTAATACA GATACAGCGG CCGGCCTGCT CATGCGCAGC 
ATCCACTTGG TCACCCAGAG ACTCAACTCG CAGTGGAGGC CGGACATGAG CATCTCGCTG 
GCTGCCTTGG AACTGCTGTC CGGACTGGCC AAGGTAAAGG TGGGTATAGA CTCTTCAGAC 
AGGAAGAGGG CAGTGAGTTC TGTGTGCACC TACATCGTAT ACCAGTGCTC CCGCCCGGCT 
CCGCATCACT CCCGGGATCT GCACTCCATG ATTGTAGCTG CCTTCCAGTG CCTGTGTGTG 
TGGCTTACAG AGCATCCAGA CATGTTAAAT GAAAAGGATT GTCTAAAAGA GGTTCTAGAG 
ATTGTAGAAC TGGGCATATC CGGTAGCAAG TCAAAAAATC CTGAAATGCA AGAGGTGCGT 
TACAAGGGGG ATAAGGAGCC CAACCCAGCC TCTATGCGGG TGAAGGACGC TGCAGAAGCA 
ACACTTACAT GTATCATGCA GCTGCTTGGG GCTTTCCCTC CGCCCAGCGG CCCGGCCTCG 
CCCTGCAGCT TGGTAAATGA GATGACGCTC ATCACATACT CTAGGCTGCC CACGGTCAGC 
AAATACAGCT TCCGATACTT TGTGCTGGAC AACAGTGTTA TACTGGCCAT GCTGGAGCAG 
CCCCTGGGCA ACGAGCAAAG TAACCCCAGT CCATCAATCA CAGTTCTGAT CCGAGGGATG 
TCTGGCCGCC ATGCATGGTC TATGCAGCTG TGTCACCTTC CAAGAGCTGC AAGAGCCAAT 
CAGAGGATGT TTATCCCCGA GCCGAGGCCG GAGCCTAATA ACTACGTAGG CATCAAATAC 
AACGTAAAGC ATCGTCCTTT CCCAGAGGAG GTGGATAAGA TTCCCTTTGT TAAGGCCGAT 
CTGAGCATCC CTGATTTACA CGAGATTGTC AATGAAGAGT TGGAAGAAAG GCACGAAAAG 
TTGAAGCACG GGATGGCCAA ACAGATAGCT TATGAGGCCG AGCTGGACCA CCTGAAAGAG 
GAGGAGCTTA AAAAGAGAAG TTATCCTGAC CCTGTAACGG AGTGCAAGCC TCCCCATCCA 
GCCCAGGAGT TCCAGACTGC CCGCCTGCTC CTCTCCCACT TTGGCTTCCT CTCTCTTGAA 
GCACTTAAAG AACCTGCAAA CAGCCGCCTA CCTCCTCACC TCATTGCACT TGACTCACAC 
ATGCCAGGAT TCTTTGAAGA TATTGGGTAC TTGGACCTAT TGCCCTGCCG CCCCTTCGAT 
ACTGTTTTTA TCTTCTACAT GAAGGCTGGA CAAAAAACAG GTCAAGAGAT CTTGAAGAAT 
GTAGAGTCTT CCAAAAACGT TCAGCCACAT TTCCTGGAGT TCCTGCTGTC CTTGGGCTGG 
CCCGTGGATG TCGGGAAGCA CCCAGGCTGG ACAGGCCATG TTTCTACCAG TTGGTCCATC 
AACTGCAGTG GTGAGAATGA GCAACACGAG CAAGAGGAAG CACCTTCGGT AGATGACACA 
GGAGGGAGCA TATTTAACGG GGAAAAGAAA GTTCTCTACT ATGCGGATGC CCTGACTGAG 
ATCGCCTTTG TTGTTCCGTC AGCTGTGGAT GCTCAAAGCG ACTCTGTGGA AAATAACACC 
TCGGAGCTTG ACGGGGACTC AAACATGGAT CTTCTCCCAG GGATACTGAA GCAGCCGCTC 
ACCCTTGAGC TTTTCCCTAC ACACGTTGAA AATGTCAGCT CAGCACAAAG GCATAGTCCC 
ACGTCGCGCA CTAAGAAGCT GCCCCAGGGG CGTGTAGTCC CACCTATGGG TCCCGAGACC 
AAGGTAGCAG TTGTATGGCT GGAGCAGTAC GATGATTTAG AAAACTTCCC TCTACCAGAC 
CTCGCAGCAG AGACCAGCAC TGGTATAGAG ACCACTACAA ACAGCAGCAC TTCTATCCGC 
AACAGTCTGC CTGAGAAAGA GTTCCCTGTG ATTTTCATCC ACCCTTTGTA CACCGGCCTC 
TTCCGAGTTA AAGTGCACGG AGCCTCTGGG AAATTCAACA TGGTGATCCC TCTGGTAGAT 
GGAATGGTGG TGAGCAGAAG AGCACTTGGT TTCTTGGTCA GACAGACCGT CATCAACATC 
TGCCGGCGGA AGCGACTGGA GAGCGACTCG TACAACACCC CGCACGTGCG TCGGAAGCAG 
AAAATCACCG ACATCGTCAA TAAGTACCGG AACCGGCAGC TGGAGCCGGA GTTTTACACC 
TCTCTCTTTC ATGACGTCGG GCTGAAGAGC TGCACCCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808230.2
CDS	194..4735
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGVAVNKKIGLERMPKYGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFEAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYGYPALNTDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_012952776.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952776.3

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA AGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCT TAAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCG 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG GATACCCAGC TCTTAATACA GATACAGCGG CCGGCCTGCT CATGCGCAGC 
ATCCACTTGG TCACCCAGAG ACTCAACTCG CAGTGGAGGC CGGACATGAG CATCTCGCTG 
GCTGCCTTGG AACTGCTGTC CGGACTGGCC AAGGTAAAGG TGGGTATAGA CTCTTCAGAC 
AGGAAGAGGG CAGTGAGTTC TGTGTGCACC TACATCGTAT ACCAGTGCTC CCGCCCGGCT 
CCGCATCACT CCCGGGATCT GCACTCCATG ATTGTAGCTG CCTTCCAGTG CCTGTGTGTG 
TGGCTTACAG AGCATCCAGA CATGTTAAAT GAAAAGGATT GTCTAAAAGA GGTTCTAGAG 
ATTGTAGAAC TGGGCATATC CGGTAGCAAG TCAAAAAATC CTGAAATGCA AGAGGTGCGT 
TACAAGGGGG ATAAGGAGCC CAACCCAGCC TCTATGCGGG TGAAGGACGC TGCAGAAGCA 
ACACTTACAT GTATCATGCA GCTGCTTGGG GCTTTCCCTC CGCCCAGCGG CCCGGCCTCG 
CCCTGCAGCT TGGTAAATGA GATGACGCTC ATCACATACT CTAGGCTGCC CACGGTCAGC 
AAATACAGCT TCCGATACTT TGTGCTGGAC AACAGTGTTA TACTGGCCAT GCTGGAGCAG 
CCCCTGGGCA ACGAGCAAAG TAACCCCAGT CCATCAATCA CAGTTCTGAT CCGAGGGATG 
TCTGGCCGCC ATGCATGGTC TATGCAGCTG TGTCACCTTC CAAGAGCTGC AAGAGCCAAT 
CAGAGGATGT TTATCCCCGA GCCGAGGCCG GAGCCTAATA ACTACGTAGG CATCAAATAC 
AACGTAAAGC ATCGTCCTTT CCCAGAGGAG GTGGATAAGA TTCCCTTTGT TAAGGCCGAT 
CTGAGCATCC CTGATTTACA CGAGATTGTC AATGAAGAGT TGGAAGAAAG GCACGAAAAG 
TTGAAGCACG GGATGGCCAA ACAGATAGCT TATGAGGCCG AGCTGGACCA CCTGAAAGAG 
GAGGAGCTTA AAAAGAGAAG TTATCCTGAC CCTGTAACGG AGTGCAAGCC TCCCCATCCA 
GCCCAGGAGT TCCAGACTGC CCGCCTGCTC CTCTCCCACT TTGGCTTCCT CTCTCTTGAA 
GCACTTAAAG AACCTGCAAA CAGCCGCCTA CCTCCTCACC TCATTGCACT TGACTCACAC 
ATGCCAGGAT TCTTTGAAGA TATTGGGTAC TTGGACCTAT TGCCCTGCCG CCCCTTCGAT 
ACTGTTTTTA TCTTCTACAT GAAGGCTGGA CAAAAAACAG GTCAAGAGAT CTTGAAGAAT 
GTAGAGTCTT CCAAAAACGT TCAGCCACAT TTCCTGGAGT TCCTGCTGTC CTTGGGCTGG 
CCCGTGGATG TCGGGAAGCA CCCAGGCTGG ACAGGCCATG TTTCTACCAG TTGGTCCATC 
AACTGCAGTG GTGAGAATGA GCAACACGAG CAAGAGGAAG CACCTTCGGT AGATGACACA 
GGAGGGAGCA TATTTAACGG GGAAAAGAAA GTTCTCTACT ATGCGGATGC CCTGACTGAG 
ATCGCCTTTG TTGTTCCGTC AGCTGTGGAT GCTCAAAGCG ACTCTGTGGA AAATAACACC 
TCGGAGCTTG ACGGGGACTC AAACATGGAT CTTCTCCCAG GGATACTGAA GCAGCCGCTC 
ACCCTTGAGC TTTTCCCTAC ACACGTTGAA AATGTCAGCT CAGCACAAAG GCATAGTCCC 
ACGTCGCGCA CTAAGAAGCT GCCCCAGGGG CGTGTAGTCC CACCTATGGG TCCCGAGACC 
AAGGTAGCAG TTGTATGGCT GGAGCAGTAC GATGATTTAG AAAACTTCCC TCTACCAGAC 
CTCGCAGCAG AGACCAGCAC TGGTATAGAG ACCACTACAA ACAGCAGCAC TTCTATCCGC 
AACAGTCTGC CTGAGAAAGA GTTCCCTGTG ATTTTCATCC ACCCTTTGTA CACCGGCCTC 
TTCCGAGTTA AAGTGCACGG AGCCTCTGGG AAATTCAACA TGGTGATCCC TCTGGTAGAT 
GGAATGGTGG TGAGCAGAAG AGCACTTGGT TTCTTGGTCA GACAGACCGT CATCAACATC 
TGCCGGCGGA AGCGACTGGA GAGCGACTCG TACAACACCC CGCACGTGCG TCGGAAGCAG 
AAAATCACCG ACATCGTCAA TAAGTACCGG AACCGGCAGC TGGAGCCGGA GTTTTACACC 
TCTCTCTTTC ATGACGTCGG GCTGAAGAGC TGCACCCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_012952778.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808232.2 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X2, mRNA.

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CloneID	OXa50223
Clone ID Related Accession (Same CDS sequence)	XM_012952778.3
Accession Version	XM_012952778.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952778.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA AGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCT TAAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCG 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG CTCTTAATAC AGATACAGCG GCCGGCCTGC TCATGCGCAG CATCCACTTG 
GTCACCCAGA GACTCAACTC GCAGTGGAGG CCGGACATGA GCATCTCGCT GGCTGCCTTG 
GAACTGCTGT CCGGACTGGC CAAGGTAAAG GTGGGTATAG ACTCTTCAGA CAGGAAGAGG 
GCAGTGAGTT CTGTGTGCAC CTACATCGTA TACCAGTGCT CCCGCCCGGC TCCGCATCAC 
TCCCGGGATC TGCACTCCAT GATTGTAGCT GCCTTCCAGT GCCTGTGTGT GTGGCTTACA 
GAGCATCCAG ACATGTTAAA TGAAAAGGAT TGTCTAAAAG AGGTTCTAGA GATTGTAGAA 
CTGGGCATAT CCGGTAGCAA GTCAAAAAAT CCTGAAATGC AAGAGGTGCG TTACAAGGGG 
GATAAGGAGC CCAACCCAGC CTCTATGCGG GTGAAGGACG CTGCAGAAGC AACACTTACA 
TGTATCATGC AGCTGCTTGG GGCTTTCCCT CCGCCCAGCG GCCCGGCCTC GCCCTGCAGC 
TTGGTAAATG AGATGACGCT CATCACATAC TCTAGGCTGC CCACGGTCAG CAAATACAGC 
TTCCGATACT TTGTGCTGGA CAACAGTGTT ATACTGGCCA TGCTGGAGCA GCCCCTGGGC 
AACGAGCAAA GTAACCCCAG TCCATCAATC ACAGTTCTGA TCCGAGGGAT GTCTGGCCGC 
CATGCATGGT CTATGCAGCT GTGTCACCTT CCAAGAGCTG CAAGAGCCAA TCAGAGGATG 
TTTATCCCCG AGCCGAGGCC GGAGCCTAAT AACTACGTAG GCATCAAATA CAACGTAAAG 
CATCGTCCTT TCCCAGAGGA GGTGGATAAG ATTCCCTTTG TTAAGGCCGA TCTGAGCATC 
CCTGATTTAC ACGAGATTGT CAATGAAGAG TTGGAAGAAA GGCACGAAAA GTTGAAGCAC 
GGGATGGCCA AACAGATAGC TTATGAGGCC GAGCTGGACC ACCTGAAAGA GGAGGAGCTT 
AAAAAGAGAA GTTATCCTGA CCCTGTAACG GAGTGCAAGC CTCCCCATCC AGCCCAGGAG 
TTCCAGACTG CCCGCCTGCT CCTCTCCCAC TTTGGCTTCC TCTCTCTTGA AGCACTTAAA 
GAACCTGCAA ACAGCCGCCT ACCTCCTCAC CTCATTGCAC TTGACTCACA CATGCCAGGA 
TTCTTTGAAG ATATTGGGTA CTTGGACCTA TTGCCCTGCC GCCCCTTCGA TACTGTTTTT 
ATCTTCTACA TGAAGGCTGG ACAAAAAACA GGTCAAGAGA TCTTGAAGAA TGTAGAGTCT 
TCCAAAAACG TTCAGCCACA TTTCCTGGAG TTCCTGCTGT CCTTGGGCTG GCCCGTGGAT 
GTCGGGAAGC ACCCAGGCTG GACAGGCCAT GTTTCTACCA GTTGGTCCAT CAACTGCAGT 
GGTGAGAATG AGCAACACGA GCAAGAGGAA GCACCTTCGG TAGATGACAC AGGAGGGAGC 
ATATTTAACG GGGAAAAGAA AGTTCTCTAC TATGCGGATG CCCTGACTGA GATCGCCTTT 
GTTGTTCCGT CAGCTGTGGA TGCTCAAAGC GACTCTGTGG AAAATAACAC CTCGGAGCTT 
GACGGGGACT CAAACATGGA TCTTCTCCCA GGGATACTGA AGCAGCCGCT CACCCTTGAG 
CTTTTCCCTA CACACGTTGA AAATGTCAGC TCAGCACAAA GGCATAGTCC CACGTCGCGC 
ACTAAGAAGC TGCCCCAGGG GCGTGTAGTC CCACCTATGG GTCCCGAGAC CAAGGTAGCA 
GTTGTATGGC TGGAGCAGTA CGATGATTTA GAAAACTTCC CTCTACCAGA CCTCGCAGCA 
GAGACCAGCA CTGGTATAGA GACCACTACA AACAGCAGCA CTTCTATCCG CAACAGTCTG 
CCTGAGAAAG AGTTCCCTGT GATTTTCATC CACCCTTTGT ACACCGGCCT CTTCCGAGTT 
AAAGTGCACG GAGCCTCTGG GAAATTCAAC ATGGTGATCC CTCTGGTAGA TGGAATGGTG 
GTGAGCAGAA GAGCACTTGG TTTCTTGGTC AGACAGACCG TCATCAACAT CTGCCGGCGG 
AAGCGACTGG AGAGCGACTC GTACAACACC CCGCACGTGC GTCGGAAGCA GAAAATCACC 
GACATCGTCA ATAAGTACCG GAACCGGCAG CTGGAGCCGG AGTTTTACAC CTCTCTCTTT 
CATGACGTCG GGCTGAAGAG CTGCACCCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808232.2
CDS	194..4726
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGVAVNKKIGLERMPKYGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFEAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYALNTDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_012952778.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952778.3

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA AGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCT TAAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCG 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG CTCTTAATAC AGATACAGCG GCCGGCCTGC TCATGCGCAG CATCCACTTG 
GTCACCCAGA GACTCAACTC GCAGTGGAGG CCGGACATGA GCATCTCGCT GGCTGCCTTG 
GAACTGCTGT CCGGACTGGC CAAGGTAAAG GTGGGTATAG ACTCTTCAGA CAGGAAGAGG 
GCAGTGAGTT CTGTGTGCAC CTACATCGTA TACCAGTGCT CCCGCCCGGC TCCGCATCAC 
TCCCGGGATC TGCACTCCAT GATTGTAGCT GCCTTCCAGT GCCTGTGTGT GTGGCTTACA 
GAGCATCCAG ACATGTTAAA TGAAAAGGAT TGTCTAAAAG AGGTTCTAGA GATTGTAGAA 
CTGGGCATAT CCGGTAGCAA GTCAAAAAAT CCTGAAATGC AAGAGGTGCG TTACAAGGGG 
GATAAGGAGC CCAACCCAGC CTCTATGCGG GTGAAGGACG CTGCAGAAGC AACACTTACA 
TGTATCATGC AGCTGCTTGG GGCTTTCCCT CCGCCCAGCG GCCCGGCCTC GCCCTGCAGC 
TTGGTAAATG AGATGACGCT CATCACATAC TCTAGGCTGC CCACGGTCAG CAAATACAGC 
TTCCGATACT TTGTGCTGGA CAACAGTGTT ATACTGGCCA TGCTGGAGCA GCCCCTGGGC 
AACGAGCAAA GTAACCCCAG TCCATCAATC ACAGTTCTGA TCCGAGGGAT GTCTGGCCGC 
CATGCATGGT CTATGCAGCT GTGTCACCTT CCAAGAGCTG CAAGAGCCAA TCAGAGGATG 
TTTATCCCCG AGCCGAGGCC GGAGCCTAAT AACTACGTAG GCATCAAATA CAACGTAAAG 
CATCGTCCTT TCCCAGAGGA GGTGGATAAG ATTCCCTTTG TTAAGGCCGA TCTGAGCATC 
CCTGATTTAC ACGAGATTGT CAATGAAGAG TTGGAAGAAA GGCACGAAAA GTTGAAGCAC 
GGGATGGCCA AACAGATAGC TTATGAGGCC GAGCTGGACC ACCTGAAAGA GGAGGAGCTT 
AAAAAGAGAA GTTATCCTGA CCCTGTAACG GAGTGCAAGC CTCCCCATCC AGCCCAGGAG 
TTCCAGACTG CCCGCCTGCT CCTCTCCCAC TTTGGCTTCC TCTCTCTTGA AGCACTTAAA 
GAACCTGCAA ACAGCCGCCT ACCTCCTCAC CTCATTGCAC TTGACTCACA CATGCCAGGA 
TTCTTTGAAG ATATTGGGTA CTTGGACCTA TTGCCCTGCC GCCCCTTCGA TACTGTTTTT 
ATCTTCTACA TGAAGGCTGG ACAAAAAACA GGTCAAGAGA TCTTGAAGAA TGTAGAGTCT 
TCCAAAAACG TTCAGCCACA TTTCCTGGAG TTCCTGCTGT CCTTGGGCTG GCCCGTGGAT 
GTCGGGAAGC ACCCAGGCTG GACAGGCCAT GTTTCTACCA GTTGGTCCAT CAACTGCAGT 
GGTGAGAATG AGCAACACGA GCAAGAGGAA GCACCTTCGG TAGATGACAC AGGAGGGAGC 
ATATTTAACG GGGAAAAGAA AGTTCTCTAC TATGCGGATG CCCTGACTGA GATCGCCTTT 
GTTGTTCCGT CAGCTGTGGA TGCTCAAAGC GACTCTGTGG AAAATAACAC CTCGGAGCTT 
GACGGGGACT CAAACATGGA TCTTCTCCCA GGGATACTGA AGCAGCCGCT CACCCTTGAG 
CTTTTCCCTA CACACGTTGA AAATGTCAGC TCAGCACAAA GGCATAGTCC CACGTCGCGC 
ACTAAGAAGC TGCCCCAGGG GCGTGTAGTC CCACCTATGG GTCCCGAGAC CAAGGTAGCA 
GTTGTATGGC TGGAGCAGTA CGATGATTTA GAAAACTTCC CTCTACCAGA CCTCGCAGCA 
GAGACCAGCA CTGGTATAGA GACCACTACA AACAGCAGCA CTTCTATCCG CAACAGTCTG 
CCTGAGAAAG AGTTCCCTGT GATTTTCATC CACCCTTTGT ACACCGGCCT CTTCCGAGTT 
AAAGTGCACG GAGCCTCTGG GAAATTCAAC ATGGTGATCC CTCTGGTAGA TGGAATGGTG 
GTGAGCAGAA GAGCACTTGG TTTCTTGGTC AGACAGACCG TCATCAACAT CTGCCGGCGG 
AAGCGACTGG AGAGCGACTC GTACAACACC CCGCACGTGC GTCGGAAGCA GAAAATCACC 
GACATCGTCA ATAAGTACCG GAACCGGCAG CTGGAGCCGG AGTTTTACAC CTCTCTCTTT 
CATGACGTCG GGCTGAAGAG CTGCACCCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_012952779.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808233.2 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa50224
Clone ID Related Accession (Same CDS sequence)	XM_012952779.3
Accession Version	XM_012952779.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952779.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA AGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCT TAAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCG 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG GATACCCAGA TACAGCGGCC GGCCTGCTCA TGCGCAGCAT CCACTTGGTC 
ACCCAGAGAC TCAACTCGCA GTGGAGGCCG GACATGAGCA TCTCGCTGGC TGCCTTGGAA 
CTGCTGTCCG GACTGGCCAA GGTAAAGGTG GGTATAGACT CTTCAGACAG GAAGAGGGCA 
GTGAGTTCTG TGTGCACCTA CATCGTATAC CAGTGCTCCC GCCCGGCTCC GCATCACTCC 
CGGGATCTGC ACTCCATGAT TGTAGCTGCC TTCCAGTGCC TGTGTGTGTG GCTTACAGAG 
CATCCAGACA TGTTAAATGA AAAGGATTGT CTAAAAGAGG TTCTAGAGAT TGTAGAACTG 
GGCATATCCG GTAGCAAGTC AAAAAATCCT GAAATGCAAG AGGTGCGTTA CAAGGGGGAT 
AAGGAGCCCA ACCCAGCCTC TATGCGGGTG AAGGACGCTG CAGAAGCAAC ACTTACATGT 
ATCATGCAGC TGCTTGGGGC TTTCCCTCCG CCCAGCGGCC CGGCCTCGCC CTGCAGCTTG 
GTAAATGAGA TGACGCTCAT CACATACTCT AGGCTGCCCA CGGTCAGCAA ATACAGCTTC 
CGATACTTTG TGCTGGACAA CAGTGTTATA CTGGCCATGC TGGAGCAGCC CCTGGGCAAC 
GAGCAAAGTA ACCCCAGTCC ATCAATCACA GTTCTGATCC GAGGGATGTC TGGCCGCCAT 
GCATGGTCTA TGCAGCTGTG TCACCTTCCA AGAGCTGCAA GAGCCAATCA GAGGATGTTT 
ATCCCCGAGC CGAGGCCGGA GCCTAATAAC TACGTAGGCA TCAAATACAA CGTAAAGCAT 
CGTCCTTTCC CAGAGGAGGT GGATAAGATT CCCTTTGTTA AGGCCGATCT GAGCATCCCT 
GATTTACACG AGATTGTCAA TGAAGAGTTG GAAGAAAGGC ACGAAAAGTT GAAGCACGGG 
ATGGCCAAAC AGATAGCTTA TGAGGCCGAG CTGGACCACC TGAAAGAGGA GGAGCTTAAA 
AAGAGAAGTT ATCCTGACCC TGTAACGGAG TGCAAGCCTC CCCATCCAGC CCAGGAGTTC 
CAGACTGCCC GCCTGCTCCT CTCCCACTTT GGCTTCCTCT CTCTTGAAGC ACTTAAAGAA 
CCTGCAAACA GCCGCCTACC TCCTCACCTC ATTGCACTTG ACTCACACAT GCCAGGATTC 
TTTGAAGATA TTGGGTACTT GGACCTATTG CCCTGCCGCC CCTTCGATAC TGTTTTTATC 
TTCTACATGA AGGCTGGACA AAAAACAGGT CAAGAGATCT TGAAGAATGT AGAGTCTTCC 
AAAAACGTTC AGCCACATTT CCTGGAGTTC CTGCTGTCCT TGGGCTGGCC CGTGGATGTC 
GGGAAGCACC CAGGCTGGAC AGGCCATGTT TCTACCAGTT GGTCCATCAA CTGCAGTGGT 
GAGAATGAGC AACACGAGCA AGAGGAAGCA CCTTCGGTAG ATGACACAGG AGGGAGCATA 
TTTAACGGGG AAAAGAAAGT TCTCTACTAT GCGGATGCCC TGACTGAGAT CGCCTTTGTT 
GTTCCGTCAG CTGTGGATGC TCAAAGCGAC TCTGTGGAAA ATAACACCTC GGAGCTTGAC 
GGGGACTCAA ACATGGATCT TCTCCCAGGG ATACTGAAGC AGCCGCTCAC CCTTGAGCTT 
TTCCCTACAC ACGTTGAAAA TGTCAGCTCA GCACAAAGGC ATAGTCCCAC GTCGCGCACT 
AAGAAGCTGC CCCAGGGGCG TGTAGTCCCA CCTATGGGTC CCGAGACCAA GGTAGCAGTT 
GTATGGCTGG AGCAGTACGA TGATTTAGAA AACTTCCCTC TACCAGACCT CGCAGCAGAG 
ACCAGCACTG GTATAGAGAC CACTACAAAC AGCAGCACTT CTATCCGCAA CAGTCTGCCT 
GAGAAAGAGT TCCCTGTGAT TTTCATCCAC CCTTTGTACA CCGGCCTCTT CCGAGTTAAA 
GTGCACGGAG CCTCTGGGAA ATTCAACATG GTGATCCCTC TGGTAGATGG AATGGTGGTG 
AGCAGAAGAG CACTTGGTTT CTTGGTCAGA CAGACCGTCA TCAACATCTG CCGGCGGAAG 
CGACTGGAGA GCGACTCGTA CAACACCCCG CACGTGCGTC GGAAGCAGAA AATCACCGAC 
ATCGTCAATA AGTACCGGAA CCGGCAGCTG GAGCCGGAGT TTTACACCTC TCTCTTTCAT 
GACGTCGGGC TGAAGAGCTG CACCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808233.2
CDS	194..4723
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGVAVNKKIGLERMPKYGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFEAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYGYPDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_012952779.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952779.3

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA AGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCT TAAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCG 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG GATACCCAGA TACAGCGGCC GGCCTGCTCA TGCGCAGCAT CCACTTGGTC 
ACCCAGAGAC TCAACTCGCA GTGGAGGCCG GACATGAGCA TCTCGCTGGC TGCCTTGGAA 
CTGCTGTCCG GACTGGCCAA GGTAAAGGTG GGTATAGACT CTTCAGACAG GAAGAGGGCA 
GTGAGTTCTG TGTGCACCTA CATCGTATAC CAGTGCTCCC GCCCGGCTCC GCATCACTCC 
CGGGATCTGC ACTCCATGAT TGTAGCTGCC TTCCAGTGCC TGTGTGTGTG GCTTACAGAG 
CATCCAGACA TGTTAAATGA AAAGGATTGT CTAAAAGAGG TTCTAGAGAT TGTAGAACTG 
GGCATATCCG GTAGCAAGTC AAAAAATCCT GAAATGCAAG AGGTGCGTTA CAAGGGGGAT 
AAGGAGCCCA ACCCAGCCTC TATGCGGGTG AAGGACGCTG CAGAAGCAAC ACTTACATGT 
ATCATGCAGC TGCTTGGGGC TTTCCCTCCG CCCAGCGGCC CGGCCTCGCC CTGCAGCTTG 
GTAAATGAGA TGACGCTCAT CACATACTCT AGGCTGCCCA CGGTCAGCAA ATACAGCTTC 
CGATACTTTG TGCTGGACAA CAGTGTTATA CTGGCCATGC TGGAGCAGCC CCTGGGCAAC 
GAGCAAAGTA ACCCCAGTCC ATCAATCACA GTTCTGATCC GAGGGATGTC TGGCCGCCAT 
GCATGGTCTA TGCAGCTGTG TCACCTTCCA AGAGCTGCAA GAGCCAATCA GAGGATGTTT 
ATCCCCGAGC CGAGGCCGGA GCCTAATAAC TACGTAGGCA TCAAATACAA CGTAAAGCAT 
CGTCCTTTCC CAGAGGAGGT GGATAAGATT CCCTTTGTTA AGGCCGATCT GAGCATCCCT 
GATTTACACG AGATTGTCAA TGAAGAGTTG GAAGAAAGGC ACGAAAAGTT GAAGCACGGG 
ATGGCCAAAC AGATAGCTTA TGAGGCCGAG CTGGACCACC TGAAAGAGGA GGAGCTTAAA 
AAGAGAAGTT ATCCTGACCC TGTAACGGAG TGCAAGCCTC CCCATCCAGC CCAGGAGTTC 
CAGACTGCCC GCCTGCTCCT CTCCCACTTT GGCTTCCTCT CTCTTGAAGC ACTTAAAGAA 
CCTGCAAACA GCCGCCTACC TCCTCACCTC ATTGCACTTG ACTCACACAT GCCAGGATTC 
TTTGAAGATA TTGGGTACTT GGACCTATTG CCCTGCCGCC CCTTCGATAC TGTTTTTATC 
TTCTACATGA AGGCTGGACA AAAAACAGGT CAAGAGATCT TGAAGAATGT AGAGTCTTCC 
AAAAACGTTC AGCCACATTT CCTGGAGTTC CTGCTGTCCT TGGGCTGGCC CGTGGATGTC 
GGGAAGCACC CAGGCTGGAC AGGCCATGTT TCTACCAGTT GGTCCATCAA CTGCAGTGGT 
GAGAATGAGC AACACGAGCA AGAGGAAGCA CCTTCGGTAG ATGACACAGG AGGGAGCATA 
TTTAACGGGG AAAAGAAAGT TCTCTACTAT GCGGATGCCC TGACTGAGAT CGCCTTTGTT 
GTTCCGTCAG CTGTGGATGC TCAAAGCGAC TCTGTGGAAA ATAACACCTC GGAGCTTGAC 
GGGGACTCAA ACATGGATCT TCTCCCAGGG ATACTGAAGC AGCCGCTCAC CCTTGAGCTT 
TTCCCTACAC ACGTTGAAAA TGTCAGCTCA GCACAAAGGC ATAGTCCCAC GTCGCGCACT 
AAGAAGCTGC CCCAGGGGCG TGTAGTCCCA CCTATGGGTC CCGAGACCAA GGTAGCAGTT 
GTATGGCTGG AGCAGTACGA TGATTTAGAA AACTTCCCTC TACCAGACCT CGCAGCAGAG 
ACCAGCACTG GTATAGAGAC CACTACAAAC AGCAGCACTT CTATCCGCAA CAGTCTGCCT 
GAGAAAGAGT TCCCTGTGAT TTTCATCCAC CCTTTGTACA CCGGCCTCTT CCGAGTTAAA 
GTGCACGGAG CCTCTGGGAA ATTCAACATG GTGATCCCTC TGGTAGATGG AATGGTGGTG 
AGCAGAAGAG CACTTGGTTT CTTGGTCAGA CAGACCGTCA TCAACATCTG CCGGCGGAAG 
CGACTGGAGA GCGACTCGTA CAACACCCCG CACGTGCGTC GGAAGCAGAA AATCACCGAC 
ATCGTCAATA AGTACCGGAA CCGGCAGCTG GAGCCGGAGT TTTACACCTC TCTCTTTCAT 
GACGTCGGGC TGAAGAGCTG CACCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_012952780.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808234.2 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa50225
Clone ID Related Accession (Same CDS sequence)	XM_012952780.3
Accession Version	XM_012952780.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952780.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA AGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCT TAAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCG 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG ATACAGCGGC CGGCCTGCTC ATGCGCAGCA TCCACTTGGT CACCCAGAGA 
CTCAACTCGC AGTGGAGGCC GGACATGAGC ATCTCGCTGG CTGCCTTGGA ACTGCTGTCC 
GGACTGGCCA AGGTAAAGGT GGGTATAGAC TCTTCAGACA GGAAGAGGGC AGTGAGTTCT 
GTGTGCACCT ACATCGTATA CCAGTGCTCC CGCCCGGCTC CGCATCACTC CCGGGATCTG 
CACTCCATGA TTGTAGCTGC CTTCCAGTGC CTGTGTGTGT GGCTTACAGA GCATCCAGAC 
ATGTTAAATG AAAAGGATTG TCTAAAAGAG GTTCTAGAGA TTGTAGAACT GGGCATATCC 
GGTAGCAAGT CAAAAAATCC TGAAATGCAA GAGGTGCGTT ACAAGGGGGA TAAGGAGCCC 
AACCCAGCCT CTATGCGGGT GAAGGACGCT GCAGAAGCAA CACTTACATG TATCATGCAG 
CTGCTTGGGG CTTTCCCTCC GCCCAGCGGC CCGGCCTCGC CCTGCAGCTT GGTAAATGAG 
ATGACGCTCA TCACATACTC TAGGCTGCCC ACGGTCAGCA AATACAGCTT CCGATACTTT 
GTGCTGGACA ACAGTGTTAT ACTGGCCATG CTGGAGCAGC CCCTGGGCAA CGAGCAAAGT 
AACCCCAGTC CATCAATCAC AGTTCTGATC CGAGGGATGT CTGGCCGCCA TGCATGGTCT 
ATGCAGCTGT GTCACCTTCC AAGAGCTGCA AGAGCCAATC AGAGGATGTT TATCCCCGAG 
CCGAGGCCGG AGCCTAATAA CTACGTAGGC ATCAAATACA ACGTAAAGCA TCGTCCTTTC 
CCAGAGGAGG TGGATAAGAT TCCCTTTGTT AAGGCCGATC TGAGCATCCC TGATTTACAC 
GAGATTGTCA ATGAAGAGTT GGAAGAAAGG CACGAAAAGT TGAAGCACGG GATGGCCAAA 
CAGATAGCTT ATGAGGCCGA GCTGGACCAC CTGAAAGAGG AGGAGCTTAA AAAGAGAAGT 
TATCCTGACC CTGTAACGGA GTGCAAGCCT CCCCATCCAG CCCAGGAGTT CCAGACTGCC 
CGCCTGCTCC TCTCCCACTT TGGCTTCCTC TCTCTTGAAG CACTTAAAGA ACCTGCAAAC 
AGCCGCCTAC CTCCTCACCT CATTGCACTT GACTCACACA TGCCAGGATT CTTTGAAGAT 
ATTGGGTACT TGGACCTATT GCCCTGCCGC CCCTTCGATA CTGTTTTTAT CTTCTACATG 
AAGGCTGGAC AAAAAACAGG TCAAGAGATC TTGAAGAATG TAGAGTCTTC CAAAAACGTT 
CAGCCACATT TCCTGGAGTT CCTGCTGTCC TTGGGCTGGC CCGTGGATGT CGGGAAGCAC 
CCAGGCTGGA CAGGCCATGT TTCTACCAGT TGGTCCATCA ACTGCAGTGG TGAGAATGAG 
CAACACGAGC AAGAGGAAGC ACCTTCGGTA GATGACACAG GAGGGAGCAT ATTTAACGGG 
GAAAAGAAAG TTCTCTACTA TGCGGATGCC CTGACTGAGA TCGCCTTTGT TGTTCCGTCA 
GCTGTGGATG CTCAAAGCGA CTCTGTGGAA AATAACACCT CGGAGCTTGA CGGGGACTCA 
AACATGGATC TTCTCCCAGG GATACTGAAG CAGCCGCTCA CCCTTGAGCT TTTCCCTACA 
CACGTTGAAA ATGTCAGCTC AGCACAAAGG CATAGTCCCA CGTCGCGCAC TAAGAAGCTG 
CCCCAGGGGC GTGTAGTCCC ACCTATGGGT CCCGAGACCA AGGTAGCAGT TGTATGGCTG 
GAGCAGTACG ATGATTTAGA AAACTTCCCT CTACCAGACC TCGCAGCAGA GACCAGCACT 
GGTATAGAGA CCACTACAAA CAGCAGCACT TCTATCCGCA ACAGTCTGCC TGAGAAAGAG 
TTCCCTGTGA TTTTCATCCA CCCTTTGTAC ACCGGCCTCT TCCGAGTTAA AGTGCACGGA 
GCCTCTGGGA AATTCAACAT GGTGATCCCT CTGGTAGATG GAATGGTGGT GAGCAGAAGA 
GCACTTGGTT TCTTGGTCAG ACAGACCGTC ATCAACATCT GCCGGCGGAA GCGACTGGAG 
AGCGACTCGT ACAACACCCC GCACGTGCGT CGGAAGCAGA AAATCACCGA CATCGTCAAT 
AAGTACCGGA ACCGGCAGCT GGAGCCGGAG TTTTACACCT CTCTCTTTCA TGACGTCGGG 
CTGAAGAGCT GCACCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808234.2
CDS	194..4714
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGVAVNKKIGLERMPKYGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFEAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_012952780.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952780.3

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA AGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCT TAAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCG 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG ATACAGCGGC CGGCCTGCTC ATGCGCAGCA TCCACTTGGT CACCCAGAGA 
CTCAACTCGC AGTGGAGGCC GGACATGAGC ATCTCGCTGG CTGCCTTGGA ACTGCTGTCC 
GGACTGGCCA AGGTAAAGGT GGGTATAGAC TCTTCAGACA GGAAGAGGGC AGTGAGTTCT 
GTGTGCACCT ACATCGTATA CCAGTGCTCC CGCCCGGCTC CGCATCACTC CCGGGATCTG 
CACTCCATGA TTGTAGCTGC CTTCCAGTGC CTGTGTGTGT GGCTTACAGA GCATCCAGAC 
ATGTTAAATG AAAAGGATTG TCTAAAAGAG GTTCTAGAGA TTGTAGAACT GGGCATATCC 
GGTAGCAAGT CAAAAAATCC TGAAATGCAA GAGGTGCGTT ACAAGGGGGA TAAGGAGCCC 
AACCCAGCCT CTATGCGGGT GAAGGACGCT GCAGAAGCAA CACTTACATG TATCATGCAG 
CTGCTTGGGG CTTTCCCTCC GCCCAGCGGC CCGGCCTCGC CCTGCAGCTT GGTAAATGAG 
ATGACGCTCA TCACATACTC TAGGCTGCCC ACGGTCAGCA AATACAGCTT CCGATACTTT 
GTGCTGGACA ACAGTGTTAT ACTGGCCATG CTGGAGCAGC CCCTGGGCAA CGAGCAAAGT 
AACCCCAGTC CATCAATCAC AGTTCTGATC CGAGGGATGT CTGGCCGCCA TGCATGGTCT 
ATGCAGCTGT GTCACCTTCC AAGAGCTGCA AGAGCCAATC AGAGGATGTT TATCCCCGAG 
CCGAGGCCGG AGCCTAATAA CTACGTAGGC ATCAAATACA ACGTAAAGCA TCGTCCTTTC 
CCAGAGGAGG TGGATAAGAT TCCCTTTGTT AAGGCCGATC TGAGCATCCC TGATTTACAC 
GAGATTGTCA ATGAAGAGTT GGAAGAAAGG CACGAAAAGT TGAAGCACGG GATGGCCAAA 
CAGATAGCTT ATGAGGCCGA GCTGGACCAC CTGAAAGAGG AGGAGCTTAA AAAGAGAAGT 
TATCCTGACC CTGTAACGGA GTGCAAGCCT CCCCATCCAG CCCAGGAGTT CCAGACTGCC 
CGCCTGCTCC TCTCCCACTT TGGCTTCCTC TCTCTTGAAG CACTTAAAGA ACCTGCAAAC 
AGCCGCCTAC CTCCTCACCT CATTGCACTT GACTCACACA TGCCAGGATT CTTTGAAGAT 
ATTGGGTACT TGGACCTATT GCCCTGCCGC CCCTTCGATA CTGTTTTTAT CTTCTACATG 
AAGGCTGGAC AAAAAACAGG TCAAGAGATC TTGAAGAATG TAGAGTCTTC CAAAAACGTT 
CAGCCACATT TCCTGGAGTT CCTGCTGTCC TTGGGCTGGC CCGTGGATGT CGGGAAGCAC 
CCAGGCTGGA CAGGCCATGT TTCTACCAGT TGGTCCATCA ACTGCAGTGG TGAGAATGAG 
CAACACGAGC AAGAGGAAGC ACCTTCGGTA GATGACACAG GAGGGAGCAT ATTTAACGGG 
GAAAAGAAAG TTCTCTACTA TGCGGATGCC CTGACTGAGA TCGCCTTTGT TGTTCCGTCA 
GCTGTGGATG CTCAAAGCGA CTCTGTGGAA AATAACACCT CGGAGCTTGA CGGGGACTCA 
AACATGGATC TTCTCCCAGG GATACTGAAG CAGCCGCTCA CCCTTGAGCT TTTCCCTACA 
CACGTTGAAA ATGTCAGCTC AGCACAAAGG CATAGTCCCA CGTCGCGCAC TAAGAAGCTG 
CCCCAGGGGC GTGTAGTCCC ACCTATGGGT CCCGAGACCA AGGTAGCAGT TGTATGGCTG 
GAGCAGTACG ATGATTTAGA AAACTTCCCT CTACCAGACC TCGCAGCAGA GACCAGCACT 
GGTATAGAGA CCACTACAAA CAGCAGCACT TCTATCCGCA ACAGTCTGCC TGAGAAAGAG 
TTCCCTGTGA TTTTCATCCA CCCTTTGTAC ACCGGCCTCT TCCGAGTTAA AGTGCACGGA 
GCCTCTGGGA AATTCAACAT GGTGATCCCT CTGGTAGATG GAATGGTGGT GAGCAGAAGA 
GCACTTGGTT TCTTGGTCAG ACAGACCGTC ATCAACATCT GCCGGCGGAA GCGACTGGAG 
AGCGACTCGT ACAACACCCC GCACGTGCGT CGGAAGCAGA AAATCACCGA CATCGTCAAT 
AAGTACCGGA ACCGGCAGCT GGAGCCGGAG TTTTACACCT CTCTCTTTCA TGACGTCGGG 
CTGAAGAGCT GCACCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_002932859.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932905.1 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X7, mRNA.

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CloneID	OXa17271
Clone ID Related Accession (Same CDS sequence)	XM_002932859.4
Accession Version	XM_002932859.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932859.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCATCTCGC GTCCCAGGGC CGACAGTGCT 
CCGCCGACCC CAGTGAACAG GCTAAGCATG CCTCAGAACG CCTCCACCAA CACTACGCCG 
CCGCACAGCC GAAGGCACCG CGCAGTCACT GTGAATAAGA CCACCGTAAA AACCAGCACG 
TCCGTCACAG CACATGGAGC AAAGGTGCAG CACCAACCGT CTTCCACCTC TCCCCTCTCC 
AGCCCCAACC AAACGAGCTC CGAGCCCCGA CCTCTCCCAG CCCCTATGAG GCCCAAAGTC 
AATAGCATCC TAAATCTGTT CGGTTCATGG CTGTTTGATG CAGCTTTGGT TCACTGCAGA 
CTGCAGAACG GAGTCAATCG AGACAGCAGC ATGACGGCAA TGACCACCCA GGCCGCCGTG 
GAGTTTCGGC GCAAAGGCTC CCAAATGTCT ACGGACACCA TGGTTTCCAG CACCGTGTTC 
GACGCGGCAG ACTTCCCGGA GAATTACGAG GCAGGAAGGG CCGAGGCCTG CGGGACCCTG 
TGTAGGATAT TCTGTAGCAA GAAAACGGGA GAGGAAATCC TGCCTGCCTA TCTGTCCAGG 
TTTTATATGG TCTTAGTCCA AGGTCTTCAG ATTGGAGACT TTATCTGCCA CCCCGTGCTA 
GCAAGCATCA TCCTGAACTC TCCGCCCCTT TTCTGCTGTG ACCTCAAGGG GATCAACGTT 
GTGGTTCCTT ACTTCATCTC TGCACTTGAA ACCATTTTGC CAGACAGGGA ACTTTCAAAG 
TTTAAAAACT ATGTGAATCC CATAGAACTC AGAAGATCCT CCATTCACAT TTTACTTTCC 
TTGCTGCCTC TGCCCCACCA CTTTGGCACC CTCAGGTCGG AGGTCCTACT GGAAGGAAAG 
TTTAGCAACG ATGACAGCTT GGGCAGCGAC AAACCGGTCA CCTTTCTTTC CCTGAAGCTG 
CGACTCGTCA ATATACTGAT AGGAGCGCTG CAAACTGAAA CGGATCCCAA CAACACACAA 
ATGATTCTAG GTGCCATGTT AAACATTGTC CAAGATTCAG CATTTCTGGA GGCAACTGGC 
AACCAGGTAG AAATGATTGA AAATAACCTA ATGAAAAGCC ACAGTCGGAC GAACAGCGGT 
ATCAGCACAG CAAGTGGCAA CAGCACAGAG CCGACCACTC CGGACAGCGA GAGACCGGCG 
CAGGCCTTGC TGAGAGACTA CGCTCTTAAT ACAGATACAG CGGCCGGCCT GCTCATGCGC 
AGCATCCACT TGGTCACCCA GAGACTCAAC TCGCAGTGGA GGCCGGACAT GAGCATCTCG 
CTGGCTGCCT TGGAACTGCT GTCCGGACTG GCCAAGGTAA AGGTGGGTAT AGACTCTTCA 
GACAGGAAGA GGGCAGTGAG TTCTGTGTGC ACCTACATCG TATACCAGTG CTCCCGCCCG 
GCTCCGCATC ACTCCCGGGA TCTGCACTCC ATGATTGTAG CTGCCTTCCA GTGCCTGTGT 
GTGTGGCTTA CAGAGCATCC AGACATGTTA AATGAAAAGG ATTGTCTAAA AGAGGTTCTA 
GAGATTGTAG AACTGGGCAT ATCCGGTAGC AAGTCAAAAA ATCCCGAAAT GCAAGAGGTG 
CGTTACAAGG GGGATAAGGA GCCCAACCCA GCCTCTATGC GGGTGAAGGA CGCTGCAGAA 
GCAACACTTA CATGTATCAT GCAGCTGCTT GGGGCTTTCC CTCCGCCCAG CGGCCCGGCC 
TCGCCCTGCA GCTTGGTAAA TGAGATGACG CTCATCACAT ACTCTAGGCT GCCCACGGTC 
AGCAAATACA GCTTCCGATA CTTTGTGCTG GACAACAGTG TTATACTGGC CATGCTGGAG 
CAGCCCCTGG GCAACGAGCA AAGTAACCCC AGTCCATCAA TCACAGTTCT GATCCGAGGG 
ATGTCTGGCC GCCATGCATG GTCTATGCAG CTGTGTCACC TTCCAAGAGC TGCAAGAGCC 
AATCAGAGGA TGTTTATCCC CGAGCCGAGG CCGGAGCCTA ATAACTACGT AGGCATCAAA 
TACAACGTAA AGCATCGTCC TTTCCCAGAG GAGGTGGATA AGATTCCCTT TGTTAAGGCC 
GATCTGAGCA TCCCTGATTT ACACGAGATT GTCAATGAAG AGTTGGAAGA AAGGCACGAA 
AAGTTGAAGC ACGGGATGGC CAAACAGATA GCTTATGAGG CCGAGCTGGA CCACCTGAAA 
GAGGAGGAGC TTAAAAAGAG AAGTTATCCT GACCCTGTAA CGGAGTGCAA GCCTCCCCAT 
CCGGCCCAGG AGTTCCAGAC CGCCCGCCTG CTCCTCTCCC ACTTTGGCTT CCTCTCTCTT 
GAAGCACTTA AAGAACCTGC AAACAGCCGC CTACCTCCTC ACCTCATTGC ACTTGACTCA 
CACATGCCAG GATTCTTTGA AGATATTGGG TACTTGGACC TATTGCCCTG CCGCCCCTTC 
GATACTGTTT TTATCTTCTA CATGAAGGCT GGACAAAAAA CAGGTCAAGA GATCTTGAAG 
AATGTAGAGT CTTCCAAAAA CGTTCAGCCA CATTTCCTGG AGTTCCTGCT GTCCTTGGGC 
TGGCCCGTGG ATGTCGGGAA GCACCCAGGC TGGACAGGCC ATGTTTCTAC CAGTTGGTCC 
ATCAACTGCA GTGGTGAGAA TGAGCAACAC GAGCAAGAGG AAGCACCTTC GGTAGATGAC 
ACAGGAGGGA GCATATTTAA CGGGGAAAAG AAAGTTCTCT ACTATGCGGA TGCCCTGACT 
GAGATCGCCT TTGTTGTTCC GTCAGCTGTG GATGCTCAAA GCGACTCTGT GGAAAATAAC 
ACCTCGGAGC TTGACGGGGA CTCAAACATG GATCTTCTCC CAGGGATACT GAAGCAGCCG 
CTCACCCTTG AGCTTTTCCC TACACACGTT GAAAATGTCA GCTCAGCACA AAGGCATAGT 
CCCACGTCGC GCACTAAGAA GCTGCCCCAG GGGCGTGTAG TCCCACCTAT GGGTCCCGAG 
ACCAAGGTAG CAGTTGTATG GCTGGAGCAG TACGATGATT TAGAAAACTT CCCTCTACCA 
GACCTCGCAG CAGAGACCAG CACTGGTATA GAGACCACTA CAAACAGCAG CACTTCTATC 
CGCAACAGTC TGCCTGAGAA AGAGTTCCCT GTGATTTTCA TCCACCCTTT GTACACCGGC 
CTCTTCCGAG TTAAAGTGCA CGGAGCCTCT GGGAAATTCA ACATGGTGAT CCCTCTGGTA 
GATGGAATGG TGGTGAGCAG AAGAGCACTT GGTTTCTTGG TCAGACAGAC CGTCATCAAC 
ATCTGCCGGC GGAAGCGACT GGAGAGCGAC TCGTACAACA CCCCGCACGT GCGTCGGAAG 
CAGAAAATCA CCGACATCGT CAATAAGTAC CGGAACCGGC AGCTGGAGCC GGAGTTTTAC 
ACCTCTCTCT TTCATGACGT CGGGCTGAAG AGCTGCACCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932905.1
CDS	195..4679
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFDAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYALNTDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_002932859.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932859.4

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCATCTCGC GTCCCAGGGC CGACAGTGCT 
CCGCCGACCC CAGTGAACAG GCTAAGCATG CCTCAGAACG CCTCCACCAA CACTACGCCG 
CCGCACAGCC GAAGGCACCG CGCAGTCACT GTGAATAAGA CCACCGTAAA AACCAGCACG 
TCCGTCACAG CACATGGAGC AAAGGTGCAG CACCAACCGT CTTCCACCTC TCCCCTCTCC 
AGCCCCAACC AAACGAGCTC CGAGCCCCGA CCTCTCCCAG CCCCTATGAG GCCCAAAGTC 
AATAGCATCC TAAATCTGTT CGGTTCATGG CTGTTTGATG CAGCTTTGGT TCACTGCAGA 
CTGCAGAACG GAGTCAATCG AGACAGCAGC ATGACGGCAA TGACCACCCA GGCCGCCGTG 
GAGTTTCGGC GCAAAGGCTC CCAAATGTCT ACGGACACCA TGGTTTCCAG CACCGTGTTC 
GACGCGGCAG ACTTCCCGGA GAATTACGAG GCAGGAAGGG CCGAGGCCTG CGGGACCCTG 
TGTAGGATAT TCTGTAGCAA GAAAACGGGA GAGGAAATCC TGCCTGCCTA TCTGTCCAGG 
TTTTATATGG TCTTAGTCCA AGGTCTTCAG ATTGGAGACT TTATCTGCCA CCCCGTGCTA 
GCAAGCATCA TCCTGAACTC TCCGCCCCTT TTCTGCTGTG ACCTCAAGGG GATCAACGTT 
GTGGTTCCTT ACTTCATCTC TGCACTTGAA ACCATTTTGC CAGACAGGGA ACTTTCAAAG 
TTTAAAAACT ATGTGAATCC CATAGAACTC AGAAGATCCT CCATTCACAT TTTACTTTCC 
TTGCTGCCTC TGCCCCACCA CTTTGGCACC CTCAGGTCGG AGGTCCTACT GGAAGGAAAG 
TTTAGCAACG ATGACAGCTT GGGCAGCGAC AAACCGGTCA CCTTTCTTTC CCTGAAGCTG 
CGACTCGTCA ATATACTGAT AGGAGCGCTG CAAACTGAAA CGGATCCCAA CAACACACAA 
ATGATTCTAG GTGCCATGTT AAACATTGTC CAAGATTCAG CATTTCTGGA GGCAACTGGC 
AACCAGGTAG AAATGATTGA AAATAACCTA ATGAAAAGCC ACAGTCGGAC GAACAGCGGT 
ATCAGCACAG CAAGTGGCAA CAGCACAGAG CCGACCACTC CGGACAGCGA GAGACCGGCG 
CAGGCCTTGC TGAGAGACTA CGCTCTTAAT ACAGATACAG CGGCCGGCCT GCTCATGCGC 
AGCATCCACT TGGTCACCCA GAGACTCAAC TCGCAGTGGA GGCCGGACAT GAGCATCTCG 
CTGGCTGCCT TGGAACTGCT GTCCGGACTG GCCAAGGTAA AGGTGGGTAT AGACTCTTCA 
GACAGGAAGA GGGCAGTGAG TTCTGTGTGC ACCTACATCG TATACCAGTG CTCCCGCCCG 
GCTCCGCATC ACTCCCGGGA TCTGCACTCC ATGATTGTAG CTGCCTTCCA GTGCCTGTGT 
GTGTGGCTTA CAGAGCATCC AGACATGTTA AATGAAAAGG ATTGTCTAAA AGAGGTTCTA 
GAGATTGTAG AACTGGGCAT ATCCGGTAGC AAGTCAAAAA ATCCCGAAAT GCAAGAGGTG 
CGTTACAAGG GGGATAAGGA GCCCAACCCA GCCTCTATGC GGGTGAAGGA CGCTGCAGAA 
GCAACACTTA CATGTATCAT GCAGCTGCTT GGGGCTTTCC CTCCGCCCAG CGGCCCGGCC 
TCGCCCTGCA GCTTGGTAAA TGAGATGACG CTCATCACAT ACTCTAGGCT GCCCACGGTC 
AGCAAATACA GCTTCCGATA CTTTGTGCTG GACAACAGTG TTATACTGGC CATGCTGGAG 
CAGCCCCTGG GCAACGAGCA AAGTAACCCC AGTCCATCAA TCACAGTTCT GATCCGAGGG 
ATGTCTGGCC GCCATGCATG GTCTATGCAG CTGTGTCACC TTCCAAGAGC TGCAAGAGCC 
AATCAGAGGA TGTTTATCCC CGAGCCGAGG CCGGAGCCTA ATAACTACGT AGGCATCAAA 
TACAACGTAA AGCATCGTCC TTTCCCAGAG GAGGTGGATA AGATTCCCTT TGTTAAGGCC 
GATCTGAGCA TCCCTGATTT ACACGAGATT GTCAATGAAG AGTTGGAAGA AAGGCACGAA 
AAGTTGAAGC ACGGGATGGC CAAACAGATA GCTTATGAGG CCGAGCTGGA CCACCTGAAA 
GAGGAGGAGC TTAAAAAGAG AAGTTATCCT GACCCTGTAA CGGAGTGCAA GCCTCCCCAT 
CCGGCCCAGG AGTTCCAGAC CGCCCGCCTG CTCCTCTCCC ACTTTGGCTT CCTCTCTCTT 
GAAGCACTTA AAGAACCTGC AAACAGCCGC CTACCTCCTC ACCTCATTGC ACTTGACTCA 
CACATGCCAG GATTCTTTGA AGATATTGGG TACTTGGACC TATTGCCCTG CCGCCCCTTC 
GATACTGTTT TTATCTTCTA CATGAAGGCT GGACAAAAAA CAGGTCAAGA GATCTTGAAG 
AATGTAGAGT CTTCCAAAAA CGTTCAGCCA CATTTCCTGG AGTTCCTGCT GTCCTTGGGC 
TGGCCCGTGG ATGTCGGGAA GCACCCAGGC TGGACAGGCC ATGTTTCTAC CAGTTGGTCC 
ATCAACTGCA GTGGTGAGAA TGAGCAACAC GAGCAAGAGG AAGCACCTTC GGTAGATGAC 
ACAGGAGGGA GCATATTTAA CGGGGAAAAG AAAGTTCTCT ACTATGCGGA TGCCCTGACT 
GAGATCGCCT TTGTTGTTCC GTCAGCTGTG GATGCTCAAA GCGACTCTGT GGAAAATAAC 
ACCTCGGAGC TTGACGGGGA CTCAAACATG GATCTTCTCC CAGGGATACT GAAGCAGCCG 
CTCACCCTTG AGCTTTTCCC TACACACGTT GAAAATGTCA GCTCAGCACA AAGGCATAGT 
CCCACGTCGC GCACTAAGAA GCTGCCCCAG GGGCGTGTAG TCCCACCTAT GGGTCCCGAG 
ACCAAGGTAG CAGTTGTATG GCTGGAGCAG TACGATGATT TAGAAAACTT CCCTCTACCA 
GACCTCGCAG CAGAGACCAG CACTGGTATA GAGACCACTA CAAACAGCAG CACTTCTATC 
CGCAACAGTC TGCCTGAGAA AGAGTTCCCT GTGATTTTCA TCCACCCTTT GTACACCGGC 
CTCTTCCGAG TTAAAGTGCA CGGAGCCTCT GGGAAATTCA ACATGGTGAT CCCTCTGGTA 
GATGGAATGG TGGTGAGCAG AAGAGCACTT GGTTTCTTGG TCAGACAGAC CGTCATCAAC 
ATCTGCCGGC GGAAGCGACT GGAGAGCGAC TCGTACAACA CCCCGCACGT GCGTCGGAAG 
CAGAAAATCA CCGACATCGT CAATAAGTAC CGGAACCGGC AGCTGGAGCC GGAGTTTTAC 
ACCTCTCTCT TTCATGACGT CGGGCTGAAG AGCTGCACCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_004918518.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918575.1 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X8, mRNA.

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CloneID	OXa17272
Clone ID Related Accession (Same CDS sequence)	XM_004918518.3
Accession Version	XM_004918518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004918518.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCATCTCGC GTCCCAGGGC CGACAGTGCT 
CCGCCGACCC CAGTGAACAG GCTAAGCATG CCTCAGAACG CCTCCACCAA CACTACGCCG 
CCGCACAGCC GAAGGCACCG CGCAGTCACT GTGAATAAGA CCACCGTAAA AACCAGCACG 
TCCGTCACAG CACATGGAGC AAAGGTGCAG CACCAACCGT CTTCCACCTC TCCCCTCTCC 
AGCCCCAACC AAACGAGCTC CGAGCCCCGA CCTCTCCCAG CCCCTATGAG GCCCAAAGTC 
AATAGCATCC TAAATCTGTT CGGTTCATGG CTGTTTGATG CAGCTTTGGT TCACTGCAGA 
CTGCAGAACG GAGTCAATCG AGACAGCAGC ATGACGGCAA TGACCACCCA GGCCGCCGTG 
GAGTTTCGGC GCAAAGGCTC CCAAATGTCT ACGGACACCA TGGTTTCCAG CACCGTGTTC 
GACGCGGCAG ACTTCCCGGA GAATTACGAG GCAGGAAGGG CCGAGGCCTG CGGGACCCTG 
TGTAGGATAT TCTGTAGCAA GAAAACGGGA GAGGAAATCC TGCCTGCCTA TCTGTCCAGG 
TTTTATATGG TCTTAGTCCA AGGTCTTCAG ATTGGAGACT TTATCTGCCA CCCCGTGCTA 
GCAAGCATCA TCCTGAACTC TCCGCCCCTT TTCTGCTGTG ACCTCAAGGG GATCAACGTT 
GTGGTTCCTT ACTTCATCTC TGCACTTGAA ACCATTTTGC CAGACAGGGA ACTTTCAAAG 
TTTAAAAACT ATGTGAATCC CATAGAACTC AGAAGATCCT CCATTCACAT TTTACTTTCC 
TTGCTGCCTC TGCCCCACCA CTTTGGCACC CTCAGGTCGG AGGTCCTACT GGAAGGAAAG 
TTTAGCAACG ATGACAGCTT GGGCAGCGAC AAACCGGTCA CCTTTCTTTC CCTGAAGCTG 
CGACTCGTCA ATATACTGAT AGGAGCGCTG CAAACTGAAA CGGATCCCAA CAACACACAA 
ATGATTCTAG GTGCCATGTT AAACATTGTC CAAGATTCAG CATTTCTGGA GGCAACTGGC 
AACCAGGTAG AAATGATTGA AAATAACCTA ATGAAAAGCC ACAGTCGGAC GAACAGCGGT 
ATCAGCACAG CAAGTGGCAA CAGCACAGAG CCGACCACTC CGGACAGCGA GAGACCGGCG 
CAGGCCTTGC TGAGAGACTA CGATACAGCG GCCGGCCTGC TCATGCGCAG CATCCACTTG 
GTCACCCAGA GACTCAACTC GCAGTGGAGG CCGGACATGA GCATCTCGCT GGCTGCCTTG 
GAACTGCTGT CCGGACTGGC CAAGGTAAAG GTGGGTATAG ACTCTTCAGA CAGGAAGAGG 
GCAGTGAGTT CTGTGTGCAC CTACATCGTA TACCAGTGCT CCCGCCCGGC TCCGCATCAC 
TCCCGGGATC TGCACTCCAT GATTGTAGCT GCCTTCCAGT GCCTGTGTGT GTGGCTTACA 
GAGCATCCAG ACATGTTAAA TGAAAAGGAT TGTCTAAAAG AGGTTCTAGA GATTGTAGAA 
CTGGGCATAT CCGGTAGCAA GTCAAAAAAT CCCGAAATGC AAGAGGTGCG TTACAAGGGG 
GATAAGGAGC CCAACCCAGC CTCTATGCGG GTGAAGGACG CTGCAGAAGC AACACTTACA 
TGTATCATGC AGCTGCTTGG GGCTTTCCCT CCGCCCAGCG GCCCGGCCTC GCCCTGCAGC 
TTGGTAAATG AGATGACGCT CATCACATAC TCTAGGCTGC CCACGGTCAG CAAATACAGC 
TTCCGATACT TTGTGCTGGA CAACAGTGTT ATACTGGCCA TGCTGGAGCA GCCCCTGGGC 
AACGAGCAAA GTAACCCCAG TCCATCAATC ACAGTTCTGA TCCGAGGGAT GTCTGGCCGC 
CATGCATGGT CTATGCAGCT GTGTCACCTT CCAAGAGCTG CAAGAGCCAA TCAGAGGATG 
TTTATCCCCG AGCCGAGGCC GGAGCCTAAT AACTACGTAG GCATCAAATA CAACGTAAAG 
CATCGTCCTT TCCCAGAGGA GGTGGATAAG ATTCCCTTTG TTAAGGCCGA TCTGAGCATC 
CCTGATTTAC ACGAGATTGT CAATGAAGAG TTGGAAGAAA GGCACGAAAA GTTGAAGCAC 
GGGATGGCCA AACAGATAGC TTATGAGGCC GAGCTGGACC ACCTGAAAGA GGAGGAGCTT 
AAAAAGAGAA GTTATCCTGA CCCTGTAACG GAGTGCAAGC CTCCCCATCC GGCCCAGGAG 
TTCCAGACCG CCCGCCTGCT CCTCTCCCAC TTTGGCTTCC TCTCTCTTGA AGCACTTAAA 
GAACCTGCAA ACAGCCGCCT ACCTCCTCAC CTCATTGCAC TTGACTCACA CATGCCAGGA 
TTCTTTGAAG ATATTGGGTA CTTGGACCTA TTGCCCTGCC GCCCCTTCGA TACTGTTTTT 
ATCTTCTACA TGAAGGCTGG ACAAAAAACA GGTCAAGAGA TCTTGAAGAA TGTAGAGTCT 
TCCAAAAACG TTCAGCCACA TTTCCTGGAG TTCCTGCTGT CCTTGGGCTG GCCCGTGGAT 
GTCGGGAAGC ACCCAGGCTG GACAGGCCAT GTTTCTACCA GTTGGTCCAT CAACTGCAGT 
GGTGAGAATG AGCAACACGA GCAAGAGGAA GCACCTTCGG TAGATGACAC AGGAGGGAGC 
ATATTTAACG GGGAAAAGAA AGTTCTCTAC TATGCGGATG CCCTGACTGA GATCGCCTTT 
GTTGTTCCGT CAGCTGTGGA TGCTCAAAGC GACTCTGTGG AAAATAACAC CTCGGAGCTT 
GACGGGGACT CAAACATGGA TCTTCTCCCA GGGATACTGA AGCAGCCGCT CACCCTTGAG 
CTTTTCCCTA CACACGTTGA AAATGTCAGC TCAGCACAAA GGCATAGTCC CACGTCGCGC 
ACTAAGAAGC TGCCCCAGGG GCGTGTAGTC CCACCTATGG GTCCCGAGAC CAAGGTAGCA 
GTTGTATGGC TGGAGCAGTA CGATGATTTA GAAAACTTCC CTCTACCAGA CCTCGCAGCA 
GAGACCAGCA CTGGTATAGA GACCACTACA AACAGCAGCA CTTCTATCCG CAACAGTCTG 
CCTGAGAAAG AGTTCCCTGT GATTTTCATC CACCCTTTGT ACACCGGCCT CTTCCGAGTT 
AAAGTGCACG GAGCCTCTGG GAAATTCAAC ATGGTGATCC CTCTGGTAGA TGGAATGGTG 
GTGAGCAGAA GAGCACTTGG TTTCTTGGTC AGACAGACCG TCATCAACAT CTGCCGGCGG 
AAGCGACTGG AGAGCGACTC GTACAACACC CCGCACGTGC GTCGGAAGCA GAAAATCACC 
GACATCGTCA ATAAGTACCG GAACCGGCAG CTGGAGCCGG AGTTTTACAC CTCTCTCTTT 
CATGACGTCG GGCTGAAGAG CTGCACCCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918575.1
CDS	195..4667
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFDAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_004918518.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918518.3

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCATCTCGC GTCCCAGGGC CGACAGTGCT 
CCGCCGACCC CAGTGAACAG GCTAAGCATG CCTCAGAACG CCTCCACCAA CACTACGCCG 
CCGCACAGCC GAAGGCACCG CGCAGTCACT GTGAATAAGA CCACCGTAAA AACCAGCACG 
TCCGTCACAG CACATGGAGC AAAGGTGCAG CACCAACCGT CTTCCACCTC TCCCCTCTCC 
AGCCCCAACC AAACGAGCTC CGAGCCCCGA CCTCTCCCAG CCCCTATGAG GCCCAAAGTC 
AATAGCATCC TAAATCTGTT CGGTTCATGG CTGTTTGATG CAGCTTTGGT TCACTGCAGA 
CTGCAGAACG GAGTCAATCG AGACAGCAGC ATGACGGCAA TGACCACCCA GGCCGCCGTG 
GAGTTTCGGC GCAAAGGCTC CCAAATGTCT ACGGACACCA TGGTTTCCAG CACCGTGTTC 
GACGCGGCAG ACTTCCCGGA GAATTACGAG GCAGGAAGGG CCGAGGCCTG CGGGACCCTG 
TGTAGGATAT TCTGTAGCAA GAAAACGGGA GAGGAAATCC TGCCTGCCTA TCTGTCCAGG 
TTTTATATGG TCTTAGTCCA AGGTCTTCAG ATTGGAGACT TTATCTGCCA CCCCGTGCTA 
GCAAGCATCA TCCTGAACTC TCCGCCCCTT TTCTGCTGTG ACCTCAAGGG GATCAACGTT 
GTGGTTCCTT ACTTCATCTC TGCACTTGAA ACCATTTTGC CAGACAGGGA ACTTTCAAAG 
TTTAAAAACT ATGTGAATCC CATAGAACTC AGAAGATCCT CCATTCACAT TTTACTTTCC 
TTGCTGCCTC TGCCCCACCA CTTTGGCACC CTCAGGTCGG AGGTCCTACT GGAAGGAAAG 
TTTAGCAACG ATGACAGCTT GGGCAGCGAC AAACCGGTCA CCTTTCTTTC CCTGAAGCTG 
CGACTCGTCA ATATACTGAT AGGAGCGCTG CAAACTGAAA CGGATCCCAA CAACACACAA 
ATGATTCTAG GTGCCATGTT AAACATTGTC CAAGATTCAG CATTTCTGGA GGCAACTGGC 
AACCAGGTAG AAATGATTGA AAATAACCTA ATGAAAAGCC ACAGTCGGAC GAACAGCGGT 
ATCAGCACAG CAAGTGGCAA CAGCACAGAG CCGACCACTC CGGACAGCGA GAGACCGGCG 
CAGGCCTTGC TGAGAGACTA CGATACAGCG GCCGGCCTGC TCATGCGCAG CATCCACTTG 
GTCACCCAGA GACTCAACTC GCAGTGGAGG CCGGACATGA GCATCTCGCT GGCTGCCTTG 
GAACTGCTGT CCGGACTGGC CAAGGTAAAG GTGGGTATAG ACTCTTCAGA CAGGAAGAGG 
GCAGTGAGTT CTGTGTGCAC CTACATCGTA TACCAGTGCT CCCGCCCGGC TCCGCATCAC 
TCCCGGGATC TGCACTCCAT GATTGTAGCT GCCTTCCAGT GCCTGTGTGT GTGGCTTACA 
GAGCATCCAG ACATGTTAAA TGAAAAGGAT TGTCTAAAAG AGGTTCTAGA GATTGTAGAA 
CTGGGCATAT CCGGTAGCAA GTCAAAAAAT CCCGAAATGC AAGAGGTGCG TTACAAGGGG 
GATAAGGAGC CCAACCCAGC CTCTATGCGG GTGAAGGACG CTGCAGAAGC AACACTTACA 
TGTATCATGC AGCTGCTTGG GGCTTTCCCT CCGCCCAGCG GCCCGGCCTC GCCCTGCAGC 
TTGGTAAATG AGATGACGCT CATCACATAC TCTAGGCTGC CCACGGTCAG CAAATACAGC 
TTCCGATACT TTGTGCTGGA CAACAGTGTT ATACTGGCCA TGCTGGAGCA GCCCCTGGGC 
AACGAGCAAA GTAACCCCAG TCCATCAATC ACAGTTCTGA TCCGAGGGAT GTCTGGCCGC 
CATGCATGGT CTATGCAGCT GTGTCACCTT CCAAGAGCTG CAAGAGCCAA TCAGAGGATG 
TTTATCCCCG AGCCGAGGCC GGAGCCTAAT AACTACGTAG GCATCAAATA CAACGTAAAG 
CATCGTCCTT TCCCAGAGGA GGTGGATAAG ATTCCCTTTG TTAAGGCCGA TCTGAGCATC 
CCTGATTTAC ACGAGATTGT CAATGAAGAG TTGGAAGAAA GGCACGAAAA GTTGAAGCAC 
GGGATGGCCA AACAGATAGC TTATGAGGCC GAGCTGGACC ACCTGAAAGA GGAGGAGCTT 
AAAAAGAGAA GTTATCCTGA CCCTGTAACG GAGTGCAAGC CTCCCCATCC GGCCCAGGAG 
TTCCAGACCG CCCGCCTGCT CCTCTCCCAC TTTGGCTTCC TCTCTCTTGA AGCACTTAAA 
GAACCTGCAA ACAGCCGCCT ACCTCCTCAC CTCATTGCAC TTGACTCACA CATGCCAGGA 
TTCTTTGAAG ATATTGGGTA CTTGGACCTA TTGCCCTGCC GCCCCTTCGA TACTGTTTTT 
ATCTTCTACA TGAAGGCTGG ACAAAAAACA GGTCAAGAGA TCTTGAAGAA TGTAGAGTCT 
TCCAAAAACG TTCAGCCACA TTTCCTGGAG TTCCTGCTGT CCTTGGGCTG GCCCGTGGAT 
GTCGGGAAGC ACCCAGGCTG GACAGGCCAT GTTTCTACCA GTTGGTCCAT CAACTGCAGT 
GGTGAGAATG AGCAACACGA GCAAGAGGAA GCACCTTCGG TAGATGACAC AGGAGGGAGC 
ATATTTAACG GGGAAAAGAA AGTTCTCTAC TATGCGGATG CCCTGACTGA GATCGCCTTT 
GTTGTTCCGT CAGCTGTGGA TGCTCAAAGC GACTCTGTGG AAAATAACAC CTCGGAGCTT 
GACGGGGACT CAAACATGGA TCTTCTCCCA GGGATACTGA AGCAGCCGCT CACCCTTGAG 
CTTTTCCCTA CACACGTTGA AAATGTCAGC TCAGCACAAA GGCATAGTCC CACGTCGCGC 
ACTAAGAAGC TGCCCCAGGG GCGTGTAGTC CCACCTATGG GTCCCGAGAC CAAGGTAGCA 
GTTGTATGGC TGGAGCAGTA CGATGATTTA GAAAACTTCC CTCTACCAGA CCTCGCAGCA 
GAGACCAGCA CTGGTATAGA GACCACTACA AACAGCAGCA CTTCTATCCG CAACAGTCTG 
CCTGAGAAAG AGTTCCCTGT GATTTTCATC CACCCTTTGT ACACCGGCCT CTTCCGAGTT 
AAAGTGCACG GAGCCTCTGG GAAATTCAAC ATGGTGATCC CTCTGGTAGA TGGAATGGTG 
GTGAGCAGAA GAGCACTTGG TTTCTTGGTC AGACAGACCG TCATCAACAT CTGCCGGCGG 
AAGCGACTGG AGAGCGACTC GTACAACACC CCGCACGTGC GTCGGAAGCA GAAAATCACC 
GACATCGTCA ATAAGTACCG GAACCGGCAG CTGGAGCCGG AGTTTTACAC CTCTCTCTTT 
CATGACGTCG GGCTGAAGAG CTGCACCCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_012952776.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808230.1 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X1, mRNA.

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CloneID	OXa35913
Clone ID Related Accession (Same CDS sequence)	XM_012952776.2
Accession Version	XM_012952776.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA CGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCC TGAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCA 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG GATACCCAGC TCTTAATACA GATACAGCGG CCGGCCTGCT CATGCGCAGC 
ATCCACTTGG TCACCCAGAG ACTCAACTCG CAGTGGAGGC CGGACATGAG CATCTCGCTG 
GCTGCCTTGG AACTGCTGTC CGGACTGGCC AAGGTAAAGG TGGGTATAGA CTCTTCAGAC 
AGGAAGAGGG CAGTGAGTTC TGTGTGCACC TACATCGTAT ACCAGTGCTC CCGCCCGGCT 
CCGCATCACT CCCGGGATCT GCACTCCATG ATTGTAGCTG CCTTCCAGTG CCTGTGTGTG 
TGGCTTACAG AGCATCCAGA CATGTTAAAT GAAAAGGATT GTCTAAAAGA GGTTCTAGAG 
ATTGTAGAAC TGGGCATATC CGGTAGCAAG TCAAAAAATC CCGAAATGCA AGAGGTGCGT 
TACAAGGGGG ATAAGGAGCC CAACCCAGCC TCTATGCGGG TGAAGGACGC TGCAGAAGCA 
ACACTTACAT GTATCATGCA GCTGCTTGGG GCTTTCCCTC CGCCCAGCGG CCCGGCCTCG 
CCCTGCAGCT TGGTAAATGA GATGACGCTC ATCACATACT CTAGGCTGCC CACGGTCAGC 
AAATACAGCT TCCGATACTT TGTGCTGGAC AACAGTGTTA TACTGGCCAT GCTGGAGCAG 
CCCCTGGGCA ACGAGCAAAG TAACCCCAGT CCATCAATCA CAGTTCTGAT CCGAGGGATG 
TCTGGCCGCC ATGCATGGTC TATGCAGCTG TGTCACCTTC CAAGAGCTGC AAGAGCCAAT 
CAGAGGATGT TTATCCCCGA GCCGAGGCCG GAGCCTAATA ACTACGTAGG CATCAAATAC 
AACGTAAAGC ATCGTCCTTT CCCAGAGGAG GTGGATAAGA TTCCCTTTGT TAAGGCCGAT 
CTGAGCATCC CTGATTTACA CGAGATTGTC AATGAAGAGT TGGAAGAAAG GCACGAAAAG 
TTGAAGCACG GGATGGCCAA ACAGATAGCT TATGAGGCCG AGCTGGACCA CCTGAAAGAG 
GAGGAGCTTA AAAAGAGAAG TTATCCTGAC CCTGTAACGG AGTGCAAGCC TCCCCATCCG 
GCCCAGGAGT TCCAGACCGC CCGCCTGCTC CTCTCCCACT TTGGCTTCCT CTCTCTTGAA 
GCACTTAAAG AACCTGCAAA CAGCCGCCTA CCTCCTCACC TCATTGCACT TGACTCACAC 
ATGCCAGGAT TCTTTGAAGA TATTGGGTAC TTGGACCTAT TGCCCTGCCG CCCCTTCGAT 
ACTGTTTTTA TCTTCTACAT GAAGGCTGGA CAAAAAACAG GTCAAGAGAT CTTGAAGAAT 
GTAGAGTCTT CCAAAAACGT TCAGCCACAT TTCCTGGAGT TCCTGCTGTC CTTGGGCTGG 
CCCGTGGATG TCGGGAAGCA CCCAGGCTGG ACAGGCCATG TTTCTACCAG TTGGTCCATC 
AACTGCAGTG GTGAGAATGA GCAACACGAG CAAGAGGAAG CACCTTCGGT AGATGACACA 
GGAGGGAGCA TATTTAACGG GGAAAAGAAA GTTCTCTACT ATGCGGATGC CCTGACTGAG 
ATCGCCTTTG TTGTTCCGTC AGCTGTGGAT GCTCAAAGCG ACTCTGTGGA AAATAACACC 
TCGGAGCTTG ACGGGGACTC AAACATGGAT CTTCTCCCAG GGATACTGAA GCAGCCGCTC 
ACCCTTGAGC TTTTCCCTAC ACACGTTGAA AATGTCAGCT CAGCACAAAG GCATAGTCCC 
ACGTCGCGCA CTAAGAAGCT GCCCCAGGGG CGTGTAGTCC CACCTATGGG TCCCGAGACC 
AAGGTAGCAG TTGTATGGCT GGAGCAGTAC GATGATTTAG AAAACTTCCC TCTACCAGAC 
CTCGCAGCAG AGACCAGCAC TGGTATAGAG ACCACTACAA ACAGCAGCAC TTCTATCCGC 
AACAGTCTGC CTGAGAAAGA GTTCCCTGTG ATTTTCATCC ACCCTTTGTA CACCGGCCTC 
TTCCGAGTTA AAGTGCACGG AGCCTCTGGG AAATTCAACA TGGTGATCCC TCTGGTAGAT 
GGAATGGTGG TGAGCAGAAG AGCACTTGGT TTCTTGGTCA GACAGACCGT CATCAACATC 
TGCCGGCGGA AGCGACTGGA GAGCGACTCG TACAACACCC CGCACGTGCG TCGGAAGCAG 
AAAATCACCG ACATCGTCAA TAAGTACCGG AACCGGCAGC TGGAGCCGGA GTTTTACACC 
TCTCTCTTTC ATGACGTCGG GCTGAAGAGC TGCACCCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808230.1
CDS	195..4736
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGVAVNKKIGLERMPKYGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFDAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYGYPALNTDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_012952776.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952776.2

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA CGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCC TGAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCA 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG GATACCCAGC TCTTAATACA GATACAGCGG CCGGCCTGCT CATGCGCAGC 
ATCCACTTGG TCACCCAGAG ACTCAACTCG CAGTGGAGGC CGGACATGAG CATCTCGCTG 
GCTGCCTTGG AACTGCTGTC CGGACTGGCC AAGGTAAAGG TGGGTATAGA CTCTTCAGAC 
AGGAAGAGGG CAGTGAGTTC TGTGTGCACC TACATCGTAT ACCAGTGCTC CCGCCCGGCT 
CCGCATCACT CCCGGGATCT GCACTCCATG ATTGTAGCTG CCTTCCAGTG CCTGTGTGTG 
TGGCTTACAG AGCATCCAGA CATGTTAAAT GAAAAGGATT GTCTAAAAGA GGTTCTAGAG 
ATTGTAGAAC TGGGCATATC CGGTAGCAAG TCAAAAAATC CCGAAATGCA AGAGGTGCGT 
TACAAGGGGG ATAAGGAGCC CAACCCAGCC TCTATGCGGG TGAAGGACGC TGCAGAAGCA 
ACACTTACAT GTATCATGCA GCTGCTTGGG GCTTTCCCTC CGCCCAGCGG CCCGGCCTCG 
CCCTGCAGCT TGGTAAATGA GATGACGCTC ATCACATACT CTAGGCTGCC CACGGTCAGC 
AAATACAGCT TCCGATACTT TGTGCTGGAC AACAGTGTTA TACTGGCCAT GCTGGAGCAG 
CCCCTGGGCA ACGAGCAAAG TAACCCCAGT CCATCAATCA CAGTTCTGAT CCGAGGGATG 
TCTGGCCGCC ATGCATGGTC TATGCAGCTG TGTCACCTTC CAAGAGCTGC AAGAGCCAAT 
CAGAGGATGT TTATCCCCGA GCCGAGGCCG GAGCCTAATA ACTACGTAGG CATCAAATAC 
AACGTAAAGC ATCGTCCTTT CCCAGAGGAG GTGGATAAGA TTCCCTTTGT TAAGGCCGAT 
CTGAGCATCC CTGATTTACA CGAGATTGTC AATGAAGAGT TGGAAGAAAG GCACGAAAAG 
TTGAAGCACG GGATGGCCAA ACAGATAGCT TATGAGGCCG AGCTGGACCA CCTGAAAGAG 
GAGGAGCTTA AAAAGAGAAG TTATCCTGAC CCTGTAACGG AGTGCAAGCC TCCCCATCCG 
GCCCAGGAGT TCCAGACCGC CCGCCTGCTC CTCTCCCACT TTGGCTTCCT CTCTCTTGAA 
GCACTTAAAG AACCTGCAAA CAGCCGCCTA CCTCCTCACC TCATTGCACT TGACTCACAC 
ATGCCAGGAT TCTTTGAAGA TATTGGGTAC TTGGACCTAT TGCCCTGCCG CCCCTTCGAT 
ACTGTTTTTA TCTTCTACAT GAAGGCTGGA CAAAAAACAG GTCAAGAGAT CTTGAAGAAT 
GTAGAGTCTT CCAAAAACGT TCAGCCACAT TTCCTGGAGT TCCTGCTGTC CTTGGGCTGG 
CCCGTGGATG TCGGGAAGCA CCCAGGCTGG ACAGGCCATG TTTCTACCAG TTGGTCCATC 
AACTGCAGTG GTGAGAATGA GCAACACGAG CAAGAGGAAG CACCTTCGGT AGATGACACA 
GGAGGGAGCA TATTTAACGG GGAAAAGAAA GTTCTCTACT ATGCGGATGC CCTGACTGAG 
ATCGCCTTTG TTGTTCCGTC AGCTGTGGAT GCTCAAAGCG ACTCTGTGGA AAATAACACC 
TCGGAGCTTG ACGGGGACTC AAACATGGAT CTTCTCCCAG GGATACTGAA GCAGCCGCTC 
ACCCTTGAGC TTTTCCCTAC ACACGTTGAA AATGTCAGCT CAGCACAAAG GCATAGTCCC 
ACGTCGCGCA CTAAGAAGCT GCCCCAGGGG CGTGTAGTCC CACCTATGGG TCCCGAGACC 
AAGGTAGCAG TTGTATGGCT GGAGCAGTAC GATGATTTAG AAAACTTCCC TCTACCAGAC 
CTCGCAGCAG AGACCAGCAC TGGTATAGAG ACCACTACAA ACAGCAGCAC TTCTATCCGC 
AACAGTCTGC CTGAGAAAGA GTTCCCTGTG ATTTTCATCC ACCCTTTGTA CACCGGCCTC 
TTCCGAGTTA AAGTGCACGG AGCCTCTGGG AAATTCAACA TGGTGATCCC TCTGGTAGAT 
GGAATGGTGG TGAGCAGAAG AGCACTTGGT TTCTTGGTCA GACAGACCGT CATCAACATC 
TGCCGGCGGA AGCGACTGGA GAGCGACTCG TACAACACCC CGCACGTGCG TCGGAAGCAG 
AAAATCACCG ACATCGTCAA TAAGTACCGG AACCGGCAGC TGGAGCCGGA GTTTTACACC 
TCTCTCTTTC ATGACGTCGG GCTGAAGAGC TGCACCCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_012952777.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808231.1 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa35914
Clone ID Related Accession (Same CDS sequence)	XM_012952777.2
Accession Version	XM_012952777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA CGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCC TGAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCA 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG GATACCCAGC TCTTAATACA GATACAGCGG CCGGCCTGCT CATGCGCAGC 
ATCCACTTGG TCACCCAGAG ACTCAACTCG CAGTGGAGGC CGGACATGAG CATCTCGCTG 
GCTGCCTTGG AACTGCTGTC CGGACTGGCC AAGGTAAAGG TGGGTATAGA CTCTTCAGAC 
AGGAAGAGGG CAGTGAGTTC TGTGTGCACC TACATCGTAT ACCAGTGCTC CCGCCCGGCT 
CCGCATCACT CCCGGGATCT GCACTCCATG ATTGTAGCTG CCTTCCAGTG CCTGTGTGTG 
TGGCTTACAG AGCATCCAGA CATGTTAAAT GAAAAGGATT GTCTAAAAGA GGTTCTAGAG 
ATTGTAGAAC TGGGCATATC CGGTAGCAAG TCAAAAAATC CCGAAATGCA AGAGGTGCGT 
TACAAGGGGG ATAAGGAGCC CAACCCAGCC TCTATGCGGG TGAAGGACGC TGCAGAAGCA 
ACACTTACAT GTATCATGCA GCTGCTTGGG GCTTTCCCTC CGCCCAGCGG CCCGGCCTCG 
CCCTGCAGCT TGGTAAATGA GATGACGCTC ATCACATACT CTAGGCTGCC CACGGTCAGC 
AAATACAGCT TCCGATACTT TGTGCTGGAC AACAGTGTTA TACTGGCCAT GCTGGAGCAG 
CCCCTGGGCA ACGAGCAAAG TAACCCCAGT CCATCAATCA CAGTTCTGAT CCGAGGGATG 
TCTGGCCGCC ATGCATGGTC TATGCAGCTG TGTCACCTTC CAAGAGCTGC AAGAGCCAAT 
CAGAGGATGT TTATCCCCGA GCCGAGGCCG GAGCCTAATA ACTACGTAGG CATCAAATAC 
AACGTAAAGC ATCGTCCTTT CCCAGAGGAG GTGGATAAGA TTCCCTTTGT TAAGGCCGAT 
CTGAGCATCC CTGATTTACA CGAGATTGTC AATGAAGAGT TGGAAGAAAG GCACGAAAAG 
TTGAAGCACG GGATGGCCAA ACAGATAGCT TATGAGGCCG AGCTGGACCA CCTGAAAGAG 
GAGGAGCTTA AAAAGAGAAG TTATCCTGAC CCTGTAACGG AGTGCAAGCC TCCCCATCCG 
GCCCAGGAGT TCCAGACCGC CCGCCTGCTC CTCTCCCACT TTGGCTTCCT CTCTCTTGAA 
GCACTTAAAG AACCTGCAAA CAGCCGCCTA CCTCCTCACC TCATTGCACT TGACTCACAC 
ATGCCAGGAT TCTTTGAAGA TATTGGGTAC TTGGACCTAT TGCCCTGCCG CCCCTTCGAT 
ACTGTTTTTA TCTTCTACAT GAAGGCTGGA CAAAAAACAG GTCAAGAGAT CTTGAAGAAT 
GTAGAGTCTT CCAAAAACGT TCAGCCACAT TTCCTGGAGT TCCTGCTGTC CTTGGGCTGG 
CCCGTGGATG TCGGGAAGCA CCCAGGCTGG ACAGGCCATG TTTCTACCAG TTGGTCCATC 
AACTGCAGTG GTGAGAATGA GCAACACGAG CAAGAGGAAG CACCTTCGGT AGATGACACA 
GGAGGGAGCA TATTTAACGG GGAAAAGAAA GTTCTCTACT ATGCGGATGC CCTGACTGAG 
ATCGCCTTTG TTGTTCCGTC AGCTGTGGAT GCTCAAAGCG ACTCTGTGGA AAATAACACC 
TCGGAGCTTG ACGGGGACTC AAACATGGAT CTTCTCCCAG GGATACTGAA GCAGCCGCTC 
ACCCTTGAGC TTTTCCCTAC ACACGTTGAA AATGTCAGCT CAGCACAAAG GCATAGTCCC 
ACGTCGCGCA CTAAGAAGCT GCCCCAGGGG CGTGTAGTCC CACCTATGGG TCCCGAGACC 
AAGGTAGCAG TTGTATGGCT GGAGCAGTAC GATGATTTAG AAAACTTCCC TCTACCAGAC 
CTCGCAGCAG AGACCAGCAC TGGTATAGAG ACCACTACAA ACAGCAGCAC TTCTATCCGT 
CTGCCTGAGA AAGAGTTCCC TGTGATTTTC ATCCACCCTT TGTACACCGG CCTCTTCCGA 
GTTAAAGTGC ACGGAGCCTC TGGGAAATTC AACATGGTGA TCCCTCTGGT AGATGGAATG 
GTGGTGAGCA GAAGAGCACT TGGTTTCTTG GTCAGACAGA CCGTCATCAA CATCTGCCGG 
CGGAAGCGAC TGGAGAGCGA CTCGTACAAC ACCCCGCACG TGCGTCGGAA GCAGAAAATC 
ACCGACATCG TCAATAAGTA CCGGAACCGG CAGCTGGAGC CGGAGTTTTA CACCTCTCTC 
TTTCATGACG TCGGGCTGAA GAGCTGCACC CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808231.1
CDS	196..4731
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGVAVNKKIGLERMPKYGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFDAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYGYPALNTDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_012952777.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952777.2

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA CGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCC TGAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCA 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG GATACCCAGC TCTTAATACA GATACAGCGG CCGGCCTGCT CATGCGCAGC 
ATCCACTTGG TCACCCAGAG ACTCAACTCG CAGTGGAGGC CGGACATGAG CATCTCGCTG 
GCTGCCTTGG AACTGCTGTC CGGACTGGCC AAGGTAAAGG TGGGTATAGA CTCTTCAGAC 
AGGAAGAGGG CAGTGAGTTC TGTGTGCACC TACATCGTAT ACCAGTGCTC CCGCCCGGCT 
CCGCATCACT CCCGGGATCT GCACTCCATG ATTGTAGCTG CCTTCCAGTG CCTGTGTGTG 
TGGCTTACAG AGCATCCAGA CATGTTAAAT GAAAAGGATT GTCTAAAAGA GGTTCTAGAG 
ATTGTAGAAC TGGGCATATC CGGTAGCAAG TCAAAAAATC CCGAAATGCA AGAGGTGCGT 
TACAAGGGGG ATAAGGAGCC CAACCCAGCC TCTATGCGGG TGAAGGACGC TGCAGAAGCA 
ACACTTACAT GTATCATGCA GCTGCTTGGG GCTTTCCCTC CGCCCAGCGG CCCGGCCTCG 
CCCTGCAGCT TGGTAAATGA GATGACGCTC ATCACATACT CTAGGCTGCC CACGGTCAGC 
AAATACAGCT TCCGATACTT TGTGCTGGAC AACAGTGTTA TACTGGCCAT GCTGGAGCAG 
CCCCTGGGCA ACGAGCAAAG TAACCCCAGT CCATCAATCA CAGTTCTGAT CCGAGGGATG 
TCTGGCCGCC ATGCATGGTC TATGCAGCTG TGTCACCTTC CAAGAGCTGC AAGAGCCAAT 
CAGAGGATGT TTATCCCCGA GCCGAGGCCG GAGCCTAATA ACTACGTAGG CATCAAATAC 
AACGTAAAGC ATCGTCCTTT CCCAGAGGAG GTGGATAAGA TTCCCTTTGT TAAGGCCGAT 
CTGAGCATCC CTGATTTACA CGAGATTGTC AATGAAGAGT TGGAAGAAAG GCACGAAAAG 
TTGAAGCACG GGATGGCCAA ACAGATAGCT TATGAGGCCG AGCTGGACCA CCTGAAAGAG 
GAGGAGCTTA AAAAGAGAAG TTATCCTGAC CCTGTAACGG AGTGCAAGCC TCCCCATCCG 
GCCCAGGAGT TCCAGACCGC CCGCCTGCTC CTCTCCCACT TTGGCTTCCT CTCTCTTGAA 
GCACTTAAAG AACCTGCAAA CAGCCGCCTA CCTCCTCACC TCATTGCACT TGACTCACAC 
ATGCCAGGAT TCTTTGAAGA TATTGGGTAC TTGGACCTAT TGCCCTGCCG CCCCTTCGAT 
ACTGTTTTTA TCTTCTACAT GAAGGCTGGA CAAAAAACAG GTCAAGAGAT CTTGAAGAAT 
GTAGAGTCTT CCAAAAACGT TCAGCCACAT TTCCTGGAGT TCCTGCTGTC CTTGGGCTGG 
CCCGTGGATG TCGGGAAGCA CCCAGGCTGG ACAGGCCATG TTTCTACCAG TTGGTCCATC 
AACTGCAGTG GTGAGAATGA GCAACACGAG CAAGAGGAAG CACCTTCGGT AGATGACACA 
GGAGGGAGCA TATTTAACGG GGAAAAGAAA GTTCTCTACT ATGCGGATGC CCTGACTGAG 
ATCGCCTTTG TTGTTCCGTC AGCTGTGGAT GCTCAAAGCG ACTCTGTGGA AAATAACACC 
TCGGAGCTTG ACGGGGACTC AAACATGGAT CTTCTCCCAG GGATACTGAA GCAGCCGCTC 
ACCCTTGAGC TTTTCCCTAC ACACGTTGAA AATGTCAGCT CAGCACAAAG GCATAGTCCC 
ACGTCGCGCA CTAAGAAGCT GCCCCAGGGG CGTGTAGTCC CACCTATGGG TCCCGAGACC 
AAGGTAGCAG TTGTATGGCT GGAGCAGTAC GATGATTTAG AAAACTTCCC TCTACCAGAC 
CTCGCAGCAG AGACCAGCAC TGGTATAGAG ACCACTACAA ACAGCAGCAC TTCTATCCGT 
CTGCCTGAGA AAGAGTTCCC TGTGATTTTC ATCCACCCTT TGTACACCGG CCTCTTCCGA 
GTTAAAGTGC ACGGAGCCTC TGGGAAATTC AACATGGTGA TCCCTCTGGT AGATGGAATG 
GTGGTGAGCA GAAGAGCACT TGGTTTCTTG GTCAGACAGA CCGTCATCAA CATCTGCCGG 
CGGAAGCGAC TGGAGAGCGA CTCGTACAAC ACCCCGCACG TGCGTCGGAA GCAGAAAATC 
ACCGACATCG TCAATAAGTA CCGGAACCGG CAGCTGGAGC CGGAGTTTTA CACCTCTCTC 
TTTCATGACG TCGGGCTGAA GAGCTGCACC CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_012952778.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808232.1 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X3, mRNA.

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CloneID	OXa35915
Clone ID Related Accession (Same CDS sequence)	XM_012952778.2
Accession Version	XM_012952778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA CGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCC TGAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCA 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG CTCTTAATAC AGATACAGCG GCCGGCCTGC TCATGCGCAG CATCCACTTG 
GTCACCCAGA GACTCAACTC GCAGTGGAGG CCGGACATGA GCATCTCGCT GGCTGCCTTG 
GAACTGCTGT CCGGACTGGC CAAGGTAAAG GTGGGTATAG ACTCTTCAGA CAGGAAGAGG 
GCAGTGAGTT CTGTGTGCAC CTACATCGTA TACCAGTGCT CCCGCCCGGC TCCGCATCAC 
TCCCGGGATC TGCACTCCAT GATTGTAGCT GCCTTCCAGT GCCTGTGTGT GTGGCTTACA 
GAGCATCCAG ACATGTTAAA TGAAAAGGAT TGTCTAAAAG AGGTTCTAGA GATTGTAGAA 
CTGGGCATAT CCGGTAGCAA GTCAAAAAAT CCCGAAATGC AAGAGGTGCG TTACAAGGGG 
GATAAGGAGC CCAACCCAGC CTCTATGCGG GTGAAGGACG CTGCAGAAGC AACACTTACA 
TGTATCATGC AGCTGCTTGG GGCTTTCCCT CCGCCCAGCG GCCCGGCCTC GCCCTGCAGC 
TTGGTAAATG AGATGACGCT CATCACATAC TCTAGGCTGC CCACGGTCAG CAAATACAGC 
TTCCGATACT TTGTGCTGGA CAACAGTGTT ATACTGGCCA TGCTGGAGCA GCCCCTGGGC 
AACGAGCAAA GTAACCCCAG TCCATCAATC ACAGTTCTGA TCCGAGGGAT GTCTGGCCGC 
CATGCATGGT CTATGCAGCT GTGTCACCTT CCAAGAGCTG CAAGAGCCAA TCAGAGGATG 
TTTATCCCCG AGCCGAGGCC GGAGCCTAAT AACTACGTAG GCATCAAATA CAACGTAAAG 
CATCGTCCTT TCCCAGAGGA GGTGGATAAG ATTCCCTTTG TTAAGGCCGA TCTGAGCATC 
CCTGATTTAC ACGAGATTGT CAATGAAGAG TTGGAAGAAA GGCACGAAAA GTTGAAGCAC 
GGGATGGCCA AACAGATAGC TTATGAGGCC GAGCTGGACC ACCTGAAAGA GGAGGAGCTT 
AAAAAGAGAA GTTATCCTGA CCCTGTAACG GAGTGCAAGC CTCCCCATCC GGCCCAGGAG 
TTCCAGACCG CCCGCCTGCT CCTCTCCCAC TTTGGCTTCC TCTCTCTTGA AGCACTTAAA 
GAACCTGCAA ACAGCCGCCT ACCTCCTCAC CTCATTGCAC TTGACTCACA CATGCCAGGA 
TTCTTTGAAG ATATTGGGTA CTTGGACCTA TTGCCCTGCC GCCCCTTCGA TACTGTTTTT 
ATCTTCTACA TGAAGGCTGG ACAAAAAACA GGTCAAGAGA TCTTGAAGAA TGTAGAGTCT 
TCCAAAAACG TTCAGCCACA TTTCCTGGAG TTCCTGCTGT CCTTGGGCTG GCCCGTGGAT 
GTCGGGAAGC ACCCAGGCTG GACAGGCCAT GTTTCTACCA GTTGGTCCAT CAACTGCAGT 
GGTGAGAATG AGCAACACGA GCAAGAGGAA GCACCTTCGG TAGATGACAC AGGAGGGAGC 
ATATTTAACG GGGAAAAGAA AGTTCTCTAC TATGCGGATG CCCTGACTGA GATCGCCTTT 
GTTGTTCCGT CAGCTGTGGA TGCTCAAAGC GACTCTGTGG AAAATAACAC CTCGGAGCTT 
GACGGGGACT CAAACATGGA TCTTCTCCCA GGGATACTGA AGCAGCCGCT CACCCTTGAG 
CTTTTCCCTA CACACGTTGA AAATGTCAGC TCAGCACAAA GGCATAGTCC CACGTCGCGC 
ACTAAGAAGC TGCCCCAGGG GCGTGTAGTC CCACCTATGG GTCCCGAGAC CAAGGTAGCA 
GTTGTATGGC TGGAGCAGTA CGATGATTTA GAAAACTTCC CTCTACCAGA CCTCGCAGCA 
GAGACCAGCA CTGGTATAGA GACCACTACA AACAGCAGCA CTTCTATCCG CAACAGTCTG 
CCTGAGAAAG AGTTCCCTGT GATTTTCATC CACCCTTTGT ACACCGGCCT CTTCCGAGTT 
AAAGTGCACG GAGCCTCTGG GAAATTCAAC ATGGTGATCC CTCTGGTAGA TGGAATGGTG 
GTGAGCAGAA GAGCACTTGG TTTCTTGGTC AGACAGACCG TCATCAACAT CTGCCGGCGG 
AAGCGACTGG AGAGCGACTC GTACAACACC CCGCACGTGC GTCGGAAGCA GAAAATCACC 
GACATCGTCA ATAAGTACCG GAACCGGCAG CTGGAGCCGG AGTTTTACAC CTCTCTCTTT 
CATGACGTCG GGCTGAAGAG CTGCACCCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808232.1
CDS	195..4727
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGVAVNKKIGLERMPKYGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFDAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYALNTDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_012952778.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952778.2

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA CGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCC TGAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCA 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG CTCTTAATAC AGATACAGCG GCCGGCCTGC TCATGCGCAG CATCCACTTG 
GTCACCCAGA GACTCAACTC GCAGTGGAGG CCGGACATGA GCATCTCGCT GGCTGCCTTG 
GAACTGCTGT CCGGACTGGC CAAGGTAAAG GTGGGTATAG ACTCTTCAGA CAGGAAGAGG 
GCAGTGAGTT CTGTGTGCAC CTACATCGTA TACCAGTGCT CCCGCCCGGC TCCGCATCAC 
TCCCGGGATC TGCACTCCAT GATTGTAGCT GCCTTCCAGT GCCTGTGTGT GTGGCTTACA 
GAGCATCCAG ACATGTTAAA TGAAAAGGAT TGTCTAAAAG AGGTTCTAGA GATTGTAGAA 
CTGGGCATAT CCGGTAGCAA GTCAAAAAAT CCCGAAATGC AAGAGGTGCG TTACAAGGGG 
GATAAGGAGC CCAACCCAGC CTCTATGCGG GTGAAGGACG CTGCAGAAGC AACACTTACA 
TGTATCATGC AGCTGCTTGG GGCTTTCCCT CCGCCCAGCG GCCCGGCCTC GCCCTGCAGC 
TTGGTAAATG AGATGACGCT CATCACATAC TCTAGGCTGC CCACGGTCAG CAAATACAGC 
TTCCGATACT TTGTGCTGGA CAACAGTGTT ATACTGGCCA TGCTGGAGCA GCCCCTGGGC 
AACGAGCAAA GTAACCCCAG TCCATCAATC ACAGTTCTGA TCCGAGGGAT GTCTGGCCGC 
CATGCATGGT CTATGCAGCT GTGTCACCTT CCAAGAGCTG CAAGAGCCAA TCAGAGGATG 
TTTATCCCCG AGCCGAGGCC GGAGCCTAAT AACTACGTAG GCATCAAATA CAACGTAAAG 
CATCGTCCTT TCCCAGAGGA GGTGGATAAG ATTCCCTTTG TTAAGGCCGA TCTGAGCATC 
CCTGATTTAC ACGAGATTGT CAATGAAGAG TTGGAAGAAA GGCACGAAAA GTTGAAGCAC 
GGGATGGCCA AACAGATAGC TTATGAGGCC GAGCTGGACC ACCTGAAAGA GGAGGAGCTT 
AAAAAGAGAA GTTATCCTGA CCCTGTAACG GAGTGCAAGC CTCCCCATCC GGCCCAGGAG 
TTCCAGACCG CCCGCCTGCT CCTCTCCCAC TTTGGCTTCC TCTCTCTTGA AGCACTTAAA 
GAACCTGCAA ACAGCCGCCT ACCTCCTCAC CTCATTGCAC TTGACTCACA CATGCCAGGA 
TTCTTTGAAG ATATTGGGTA CTTGGACCTA TTGCCCTGCC GCCCCTTCGA TACTGTTTTT 
ATCTTCTACA TGAAGGCTGG ACAAAAAACA GGTCAAGAGA TCTTGAAGAA TGTAGAGTCT 
TCCAAAAACG TTCAGCCACA TTTCCTGGAG TTCCTGCTGT CCTTGGGCTG GCCCGTGGAT 
GTCGGGAAGC ACCCAGGCTG GACAGGCCAT GTTTCTACCA GTTGGTCCAT CAACTGCAGT 
GGTGAGAATG AGCAACACGA GCAAGAGGAA GCACCTTCGG TAGATGACAC AGGAGGGAGC 
ATATTTAACG GGGAAAAGAA AGTTCTCTAC TATGCGGATG CCCTGACTGA GATCGCCTTT 
GTTGTTCCGT CAGCTGTGGA TGCTCAAAGC GACTCTGTGG AAAATAACAC CTCGGAGCTT 
GACGGGGACT CAAACATGGA TCTTCTCCCA GGGATACTGA AGCAGCCGCT CACCCTTGAG 
CTTTTCCCTA CACACGTTGA AAATGTCAGC TCAGCACAAA GGCATAGTCC CACGTCGCGC 
ACTAAGAAGC TGCCCCAGGG GCGTGTAGTC CCACCTATGG GTCCCGAGAC CAAGGTAGCA 
GTTGTATGGC TGGAGCAGTA CGATGATTTA GAAAACTTCC CTCTACCAGA CCTCGCAGCA 
GAGACCAGCA CTGGTATAGA GACCACTACA AACAGCAGCA CTTCTATCCG CAACAGTCTG 
CCTGAGAAAG AGTTCCCTGT GATTTTCATC CACCCTTTGT ACACCGGCCT CTTCCGAGTT 
AAAGTGCACG GAGCCTCTGG GAAATTCAAC ATGGTGATCC CTCTGGTAGA TGGAATGGTG 
GTGAGCAGAA GAGCACTTGG TTTCTTGGTC AGACAGACCG TCATCAACAT CTGCCGGCGG 
AAGCGACTGG AGAGCGACTC GTACAACACC CCGCACGTGC GTCGGAAGCA GAAAATCACC 
GACATCGTCA ATAAGTACCG GAACCGGCAG CTGGAGCCGG AGTTTTACAC CTCTCTCTTT 
CATGACGTCG GGCTGAAGAG CTGCACCCCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_012952779.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808233.1 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X4, mRNA.

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CloneID	OXa35916
Clone ID Related Accession (Same CDS sequence)	XM_012952779.2
Accession Version	XM_012952779.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA CGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCC TGAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCA 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG GATACCCAGA TACAGCGGCC GGCCTGCTCA TGCGCAGCAT CCACTTGGTC 
ACCCAGAGAC TCAACTCGCA GTGGAGGCCG GACATGAGCA TCTCGCTGGC TGCCTTGGAA 
CTGCTGTCCG GACTGGCCAA GGTAAAGGTG GGTATAGACT CTTCAGACAG GAAGAGGGCA 
GTGAGTTCTG TGTGCACCTA CATCGTATAC CAGTGCTCCC GCCCGGCTCC GCATCACTCC 
CGGGATCTGC ACTCCATGAT TGTAGCTGCC TTCCAGTGCC TGTGTGTGTG GCTTACAGAG 
CATCCAGACA TGTTAAATGA AAAGGATTGT CTAAAAGAGG TTCTAGAGAT TGTAGAACTG 
GGCATATCCG GTAGCAAGTC AAAAAATCCC GAAATGCAAG AGGTGCGTTA CAAGGGGGAT 
AAGGAGCCCA ACCCAGCCTC TATGCGGGTG AAGGACGCTG CAGAAGCAAC ACTTACATGT 
ATCATGCAGC TGCTTGGGGC TTTCCCTCCG CCCAGCGGCC CGGCCTCGCC CTGCAGCTTG 
GTAAATGAGA TGACGCTCAT CACATACTCT AGGCTGCCCA CGGTCAGCAA ATACAGCTTC 
CGATACTTTG TGCTGGACAA CAGTGTTATA CTGGCCATGC TGGAGCAGCC CCTGGGCAAC 
GAGCAAAGTA ACCCCAGTCC ATCAATCACA GTTCTGATCC GAGGGATGTC TGGCCGCCAT 
GCATGGTCTA TGCAGCTGTG TCACCTTCCA AGAGCTGCAA GAGCCAATCA GAGGATGTTT 
ATCCCCGAGC CGAGGCCGGA GCCTAATAAC TACGTAGGCA TCAAATACAA CGTAAAGCAT 
CGTCCTTTCC CAGAGGAGGT GGATAAGATT CCCTTTGTTA AGGCCGATCT GAGCATCCCT 
GATTTACACG AGATTGTCAA TGAAGAGTTG GAAGAAAGGC ACGAAAAGTT GAAGCACGGG 
ATGGCCAAAC AGATAGCTTA TGAGGCCGAG CTGGACCACC TGAAAGAGGA GGAGCTTAAA 
AAGAGAAGTT ATCCTGACCC TGTAACGGAG TGCAAGCCTC CCCATCCGGC CCAGGAGTTC 
CAGACCGCCC GCCTGCTCCT CTCCCACTTT GGCTTCCTCT CTCTTGAAGC ACTTAAAGAA 
CCTGCAAACA GCCGCCTACC TCCTCACCTC ATTGCACTTG ACTCACACAT GCCAGGATTC 
TTTGAAGATA TTGGGTACTT GGACCTATTG CCCTGCCGCC CCTTCGATAC TGTTTTTATC 
TTCTACATGA AGGCTGGACA AAAAACAGGT CAAGAGATCT TGAAGAATGT AGAGTCTTCC 
AAAAACGTTC AGCCACATTT CCTGGAGTTC CTGCTGTCCT TGGGCTGGCC CGTGGATGTC 
GGGAAGCACC CAGGCTGGAC AGGCCATGTT TCTACCAGTT GGTCCATCAA CTGCAGTGGT 
GAGAATGAGC AACACGAGCA AGAGGAAGCA CCTTCGGTAG ATGACACAGG AGGGAGCATA 
TTTAACGGGG AAAAGAAAGT TCTCTACTAT GCGGATGCCC TGACTGAGAT CGCCTTTGTT 
GTTCCGTCAG CTGTGGATGC TCAAAGCGAC TCTGTGGAAA ATAACACCTC GGAGCTTGAC 
GGGGACTCAA ACATGGATCT TCTCCCAGGG ATACTGAAGC AGCCGCTCAC CCTTGAGCTT 
TTCCCTACAC ACGTTGAAAA TGTCAGCTCA GCACAAAGGC ATAGTCCCAC GTCGCGCACT 
AAGAAGCTGC CCCAGGGGCG TGTAGTCCCA CCTATGGGTC CCGAGACCAA GGTAGCAGTT 
GTATGGCTGG AGCAGTACGA TGATTTAGAA AACTTCCCTC TACCAGACCT CGCAGCAGAG 
ACCAGCACTG GTATAGAGAC CACTACAAAC AGCAGCACTT CTATCCGCAA CAGTCTGCCT 
GAGAAAGAGT TCCCTGTGAT TTTCATCCAC CCTTTGTACA CCGGCCTCTT CCGAGTTAAA 
GTGCACGGAG CCTCTGGGAA ATTCAACATG GTGATCCCTC TGGTAGATGG AATGGTGGTG 
AGCAGAAGAG CACTTGGTTT CTTGGTCAGA CAGACCGTCA TCAACATCTG CCGGCGGAAG 
CGACTGGAGA GCGACTCGTA CAACACCCCG CACGTGCGTC GGAAGCAGAA AATCACCGAC 
ATCGTCAATA AGTACCGGAA CCGGCAGCTG GAGCCGGAGT TTTACACCTC TCTCTTTCAT 
GACGTCGGGC TGAAGAGCTG CACCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808233.1
CDS	195..4724
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGVAVNKKIGLERMPKYGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFDAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYGYPDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_012952779.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952779.2

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA CGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCC TGAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCA 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG GATACCCAGA TACAGCGGCC GGCCTGCTCA TGCGCAGCAT CCACTTGGTC 
ACCCAGAGAC TCAACTCGCA GTGGAGGCCG GACATGAGCA TCTCGCTGGC TGCCTTGGAA 
CTGCTGTCCG GACTGGCCAA GGTAAAGGTG GGTATAGACT CTTCAGACAG GAAGAGGGCA 
GTGAGTTCTG TGTGCACCTA CATCGTATAC CAGTGCTCCC GCCCGGCTCC GCATCACTCC 
CGGGATCTGC ACTCCATGAT TGTAGCTGCC TTCCAGTGCC TGTGTGTGTG GCTTACAGAG 
CATCCAGACA TGTTAAATGA AAAGGATTGT CTAAAAGAGG TTCTAGAGAT TGTAGAACTG 
GGCATATCCG GTAGCAAGTC AAAAAATCCC GAAATGCAAG AGGTGCGTTA CAAGGGGGAT 
AAGGAGCCCA ACCCAGCCTC TATGCGGGTG AAGGACGCTG CAGAAGCAAC ACTTACATGT 
ATCATGCAGC TGCTTGGGGC TTTCCCTCCG CCCAGCGGCC CGGCCTCGCC CTGCAGCTTG 
GTAAATGAGA TGACGCTCAT CACATACTCT AGGCTGCCCA CGGTCAGCAA ATACAGCTTC 
CGATACTTTG TGCTGGACAA CAGTGTTATA CTGGCCATGC TGGAGCAGCC CCTGGGCAAC 
GAGCAAAGTA ACCCCAGTCC ATCAATCACA GTTCTGATCC GAGGGATGTC TGGCCGCCAT 
GCATGGTCTA TGCAGCTGTG TCACCTTCCA AGAGCTGCAA GAGCCAATCA GAGGATGTTT 
ATCCCCGAGC CGAGGCCGGA GCCTAATAAC TACGTAGGCA TCAAATACAA CGTAAAGCAT 
CGTCCTTTCC CAGAGGAGGT GGATAAGATT CCCTTTGTTA AGGCCGATCT GAGCATCCCT 
GATTTACACG AGATTGTCAA TGAAGAGTTG GAAGAAAGGC ACGAAAAGTT GAAGCACGGG 
ATGGCCAAAC AGATAGCTTA TGAGGCCGAG CTGGACCACC TGAAAGAGGA GGAGCTTAAA 
AAGAGAAGTT ATCCTGACCC TGTAACGGAG TGCAAGCCTC CCCATCCGGC CCAGGAGTTC 
CAGACCGCCC GCCTGCTCCT CTCCCACTTT GGCTTCCTCT CTCTTGAAGC ACTTAAAGAA 
CCTGCAAACA GCCGCCTACC TCCTCACCTC ATTGCACTTG ACTCACACAT GCCAGGATTC 
TTTGAAGATA TTGGGTACTT GGACCTATTG CCCTGCCGCC CCTTCGATAC TGTTTTTATC 
TTCTACATGA AGGCTGGACA AAAAACAGGT CAAGAGATCT TGAAGAATGT AGAGTCTTCC 
AAAAACGTTC AGCCACATTT CCTGGAGTTC CTGCTGTCCT TGGGCTGGCC CGTGGATGTC 
GGGAAGCACC CAGGCTGGAC AGGCCATGTT TCTACCAGTT GGTCCATCAA CTGCAGTGGT 
GAGAATGAGC AACACGAGCA AGAGGAAGCA CCTTCGGTAG ATGACACAGG AGGGAGCATA 
TTTAACGGGG AAAAGAAAGT TCTCTACTAT GCGGATGCCC TGACTGAGAT CGCCTTTGTT 
GTTCCGTCAG CTGTGGATGC TCAAAGCGAC TCTGTGGAAA ATAACACCTC GGAGCTTGAC 
GGGGACTCAA ACATGGATCT TCTCCCAGGG ATACTGAAGC AGCCGCTCAC CCTTGAGCTT 
TTCCCTACAC ACGTTGAAAA TGTCAGCTCA GCACAAAGGC ATAGTCCCAC GTCGCGCACT 
AAGAAGCTGC CCCAGGGGCG TGTAGTCCCA CCTATGGGTC CCGAGACCAA GGTAGCAGTT 
GTATGGCTGG AGCAGTACGA TGATTTAGAA AACTTCCCTC TACCAGACCT CGCAGCAGAG 
ACCAGCACTG GTATAGAGAC CACTACAAAC AGCAGCACTT CTATCCGCAA CAGTCTGCCT 
GAGAAAGAGT TCCCTGTGAT TTTCATCCAC CCTTTGTACA CCGGCCTCTT CCGAGTTAAA 
GTGCACGGAG CCTCTGGGAA ATTCAACATG GTGATCCCTC TGGTAGATGG AATGGTGGTG 
AGCAGAAGAG CACTTGGTTT CTTGGTCAGA CAGACCGTCA TCAACATCTG CCGGCGGAAG 
CGACTGGAGA GCGACTCGTA CAACACCCCG CACGTGCGTC GGAAGCAGAA AATCACCGAC 
ATCGTCAATA AGTACCGGAA CCGGCAGCTG GAGCCGGAGT TTTACACCTC TCTCTTTCAT 
GACGTCGGGC TGAAGAGCTG CACCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_012952780.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808234.1 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X5, mRNA.

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CloneID	OXa35917
Clone ID Related Accession (Same CDS sequence)	XM_012952780.2
Accession Version	XM_012952780.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA CGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCC TGAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCA 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG ATACAGCGGC CGGCCTGCTC ATGCGCAGCA TCCACTTGGT CACCCAGAGA 
CTCAACTCGC AGTGGAGGCC GGACATGAGC ATCTCGCTGG CTGCCTTGGA ACTGCTGTCC 
GGACTGGCCA AGGTAAAGGT GGGTATAGAC TCTTCAGACA GGAAGAGGGC AGTGAGTTCT 
GTGTGCACCT ACATCGTATA CCAGTGCTCC CGCCCGGCTC CGCATCACTC CCGGGATCTG 
CACTCCATGA TTGTAGCTGC CTTCCAGTGC CTGTGTGTGT GGCTTACAGA GCATCCAGAC 
ATGTTAAATG AAAAGGATTG TCTAAAAGAG GTTCTAGAGA TTGTAGAACT GGGCATATCC 
GGTAGCAAGT CAAAAAATCC CGAAATGCAA GAGGTGCGTT ACAAGGGGGA TAAGGAGCCC 
AACCCAGCCT CTATGCGGGT GAAGGACGCT GCAGAAGCAA CACTTACATG TATCATGCAG 
CTGCTTGGGG CTTTCCCTCC GCCCAGCGGC CCGGCCTCGC CCTGCAGCTT GGTAAATGAG 
ATGACGCTCA TCACATACTC TAGGCTGCCC ACGGTCAGCA AATACAGCTT CCGATACTTT 
GTGCTGGACA ACAGTGTTAT ACTGGCCATG CTGGAGCAGC CCCTGGGCAA CGAGCAAAGT 
AACCCCAGTC CATCAATCAC AGTTCTGATC CGAGGGATGT CTGGCCGCCA TGCATGGTCT 
ATGCAGCTGT GTCACCTTCC AAGAGCTGCA AGAGCCAATC AGAGGATGTT TATCCCCGAG 
CCGAGGCCGG AGCCTAATAA CTACGTAGGC ATCAAATACA ACGTAAAGCA TCGTCCTTTC 
CCAGAGGAGG TGGATAAGAT TCCCTTTGTT AAGGCCGATC TGAGCATCCC TGATTTACAC 
GAGATTGTCA ATGAAGAGTT GGAAGAAAGG CACGAAAAGT TGAAGCACGG GATGGCCAAA 
CAGATAGCTT ATGAGGCCGA GCTGGACCAC CTGAAAGAGG AGGAGCTTAA AAAGAGAAGT 
TATCCTGACC CTGTAACGGA GTGCAAGCCT CCCCATCCGG CCCAGGAGTT CCAGACCGCC 
CGCCTGCTCC TCTCCCACTT TGGCTTCCTC TCTCTTGAAG CACTTAAAGA ACCTGCAAAC 
AGCCGCCTAC CTCCTCACCT CATTGCACTT GACTCACACA TGCCAGGATT CTTTGAAGAT 
ATTGGGTACT TGGACCTATT GCCCTGCCGC CCCTTCGATA CTGTTTTTAT CTTCTACATG 
AAGGCTGGAC AAAAAACAGG TCAAGAGATC TTGAAGAATG TAGAGTCTTC CAAAAACGTT 
CAGCCACATT TCCTGGAGTT CCTGCTGTCC TTGGGCTGGC CCGTGGATGT CGGGAAGCAC 
CCAGGCTGGA CAGGCCATGT TTCTACCAGT TGGTCCATCA ACTGCAGTGG TGAGAATGAG 
CAACACGAGC AAGAGGAAGC ACCTTCGGTA GATGACACAG GAGGGAGCAT ATTTAACGGG 
GAAAAGAAAG TTCTCTACTA TGCGGATGCC CTGACTGAGA TCGCCTTTGT TGTTCCGTCA 
GCTGTGGATG CTCAAAGCGA CTCTGTGGAA AATAACACCT CGGAGCTTGA CGGGGACTCA 
AACATGGATC TTCTCCCAGG GATACTGAAG CAGCCGCTCA CCCTTGAGCT TTTCCCTACA 
CACGTTGAAA ATGTCAGCTC AGCACAAAGG CATAGTCCCA CGTCGCGCAC TAAGAAGCTG 
CCCCAGGGGC GTGTAGTCCC ACCTATGGGT CCCGAGACCA AGGTAGCAGT TGTATGGCTG 
GAGCAGTACG ATGATTTAGA AAACTTCCCT CTACCAGACC TCGCAGCAGA GACCAGCACT 
GGTATAGAGA CCACTACAAA CAGCAGCACT TCTATCCGCA ACAGTCTGCC TGAGAAAGAG 
TTCCCTGTGA TTTTCATCCA CCCTTTGTAC ACCGGCCTCT TCCGAGTTAA AGTGCACGGA 
GCCTCTGGGA AATTCAACAT GGTGATCCCT CTGGTAGATG GAATGGTGGT GAGCAGAAGA 
GCACTTGGTT TCTTGGTCAG ACAGACCGTC ATCAACATCT GCCGGCGGAA GCGACTGGAG 
AGCGACTCGT ACAACACCCC GCACGTGCGT CGGAAGCAGA AAATCACCGA CATCGTCAAT 
AAGTACCGGA ACCGGCAGCT GGAGCCGGAG TTTTACACCT CTCTCTTTCA TGACGTCGGG 
CTGAAGAGCT GCACCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808234.1
CDS	195..4715
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGVAVNKKIGLERMPKYGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFDAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_012952780.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952780.2

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCGTTGCAG TCAATAAGAA AATTGGGTTG 
GAAAGAATGC CCAAATATGG CATCTCGCGT CCCAGGGCCG ACAGTGCTCC GCCGACCCCA 
GTGAACAGGC TAAGCATGCC TCAGAACGCC TCCACCAACA CTACGCCGCC GCACAGCCGA 
AGGCACCGCG CAGTCACTGT GAATAAGACC ACCGTAAAAA CCAGCACGTC CGTCACAGCA 
CATGGAGCAA AGGTGCAGCA CCAACCGTCT TCCACCTCTC CCCTCTCCAG CCCCAACCAA 
ACGAGCTCCG AGCCCCGACC TCTCCCAGCC CCTATGAGGC CCAAAGTCAA TAGCATCCTA 
AATCTGTTCG GTTCATGGCT GTTTGATGCA GCTTTGGTTC ACTGCAGACT GCAGAACGGA 
GTCAATCGAG ACAGCAGCAT GACGGCAATG ACCACCCAGG CCGCCGTGGA GTTTCGGCGC 
AAAGGCTCCC AAATGTCTAC GGACACCATG GTTTCCAGCA CCGTGTTCGA CGCGGCAGAC 
TTCCCGGAGA ATTACGAGGC AGGAAGGGCC GAGGCCTGCG GGACCCTGTG TAGGATATTC 
TGTAGCAAGA AAACGGGAGA GGAAATCCTG CCTGCCTATC TGTCCAGGTT TTATATGGTC 
TTAGTCCAAG GTCTTCAGAT TGGAGACTTT ATCTGCCACC CCGTGCTAGC AAGCATCATC 
CTGAACTCTC CGCCCCTTTT CTGCTGTGAC CTCAAGGGGA TCAACGTTGT GGTTCCTTAC 
TTCATCTCTG CACTTGAAAC CATTTTGCCA GACAGGGAAC TTTCAAAGTT TAAAAACTAT 
GTGAATCCCA TAGAACTCAG AAGATCCTCC ATTCACATTT TACTTTCCTT GCTGCCTCTG 
CCCCACCACT TTGGCACCCT CAGGTCGGAG GTCCTACTGG AAGGAAAGTT TAGCAACGAT 
GACAGCTTGG GCAGCGACAA ACCGGTCACC TTTCTTTCCC TGAAGCTGCG ACTCGTCAAT 
ATACTGATAG GAGCGCTGCA AACTGAAACG GATCCCAACA ACACACAAAT GATTCTAGGT 
GCCATGTTAA ACATTGTCCA AGATTCAGCA TTTCTGGAGG CAACTGGCAA CCAGGTAGAA 
ATGATTGAAA ATAACCTAAT GAAAAGCCAC AGTCGGACGA ACAGCGGTAT CAGCACAGCA 
AGTGGCAACA GCACAGAGCC GACCACTCCG GACAGCGAGA GACCGGCGCA GGCCTTGCTG 
AGAGACTACG ATACAGCGGC CGGCCTGCTC ATGCGCAGCA TCCACTTGGT CACCCAGAGA 
CTCAACTCGC AGTGGAGGCC GGACATGAGC ATCTCGCTGG CTGCCTTGGA ACTGCTGTCC 
GGACTGGCCA AGGTAAAGGT GGGTATAGAC TCTTCAGACA GGAAGAGGGC AGTGAGTTCT 
GTGTGCACCT ACATCGTATA CCAGTGCTCC CGCCCGGCTC CGCATCACTC CCGGGATCTG 
CACTCCATGA TTGTAGCTGC CTTCCAGTGC CTGTGTGTGT GGCTTACAGA GCATCCAGAC 
ATGTTAAATG AAAAGGATTG TCTAAAAGAG GTTCTAGAGA TTGTAGAACT GGGCATATCC 
GGTAGCAAGT CAAAAAATCC CGAAATGCAA GAGGTGCGTT ACAAGGGGGA TAAGGAGCCC 
AACCCAGCCT CTATGCGGGT GAAGGACGCT GCAGAAGCAA CACTTACATG TATCATGCAG 
CTGCTTGGGG CTTTCCCTCC GCCCAGCGGC CCGGCCTCGC CCTGCAGCTT GGTAAATGAG 
ATGACGCTCA TCACATACTC TAGGCTGCCC ACGGTCAGCA AATACAGCTT CCGATACTTT 
GTGCTGGACA ACAGTGTTAT ACTGGCCATG CTGGAGCAGC CCCTGGGCAA CGAGCAAAGT 
AACCCCAGTC CATCAATCAC AGTTCTGATC CGAGGGATGT CTGGCCGCCA TGCATGGTCT 
ATGCAGCTGT GTCACCTTCC AAGAGCTGCA AGAGCCAATC AGAGGATGTT TATCCCCGAG 
CCGAGGCCGG AGCCTAATAA CTACGTAGGC ATCAAATACA ACGTAAAGCA TCGTCCTTTC 
CCAGAGGAGG TGGATAAGAT TCCCTTTGTT AAGGCCGATC TGAGCATCCC TGATTTACAC 
GAGATTGTCA ATGAAGAGTT GGAAGAAAGG CACGAAAAGT TGAAGCACGG GATGGCCAAA 
CAGATAGCTT ATGAGGCCGA GCTGGACCAC CTGAAAGAGG AGGAGCTTAA AAAGAGAAGT 
TATCCTGACC CTGTAACGGA GTGCAAGCCT CCCCATCCGG CCCAGGAGTT CCAGACCGCC 
CGCCTGCTCC TCTCCCACTT TGGCTTCCTC TCTCTTGAAG CACTTAAAGA ACCTGCAAAC 
AGCCGCCTAC CTCCTCACCT CATTGCACTT GACTCACACA TGCCAGGATT CTTTGAAGAT 
ATTGGGTACT TGGACCTATT GCCCTGCCGC CCCTTCGATA CTGTTTTTAT CTTCTACATG 
AAGGCTGGAC AAAAAACAGG TCAAGAGATC TTGAAGAATG TAGAGTCTTC CAAAAACGTT 
CAGCCACATT TCCTGGAGTT CCTGCTGTCC TTGGGCTGGC CCGTGGATGT CGGGAAGCAC 
CCAGGCTGGA CAGGCCATGT TTCTACCAGT TGGTCCATCA ACTGCAGTGG TGAGAATGAG 
CAACACGAGC AAGAGGAAGC ACCTTCGGTA GATGACACAG GAGGGAGCAT ATTTAACGGG 
GAAAAGAAAG TTCTCTACTA TGCGGATGCC CTGACTGAGA TCGCCTTTGT TGTTCCGTCA 
GCTGTGGATG CTCAAAGCGA CTCTGTGGAA AATAACACCT CGGAGCTTGA CGGGGACTCA 
AACATGGATC TTCTCCCAGG GATACTGAAG CAGCCGCTCA CCCTTGAGCT TTTCCCTACA 
CACGTTGAAA ATGTCAGCTC AGCACAAAGG CATAGTCCCA CGTCGCGCAC TAAGAAGCTG 
CCCCAGGGGC GTGTAGTCCC ACCTATGGGT CCCGAGACCA AGGTAGCAGT TGTATGGCTG 
GAGCAGTACG ATGATTTAGA AAACTTCCCT CTACCAGACC TCGCAGCAGA GACCAGCACT 
GGTATAGAGA CCACTACAAA CAGCAGCACT TCTATCCGCA ACAGTCTGCC TGAGAAAGAG 
TTCCCTGTGA TTTTCATCCA CCCTTTGTAC ACCGGCCTCT TCCGAGTTAA AGTGCACGGA 
GCCTCTGGGA AATTCAACAT GGTGATCCCT CTGGTAGATG GAATGGTGGT GAGCAGAAGA 
GCACTTGGTT TCTTGGTCAG ACAGACCGTC ATCAACATCT GCCGGCGGAA GCGACTGGAG 
AGCGACTCGT ACAACACCCC GCACGTGCGT CGGAAGCAGA AAATCACCGA CATCGTCAAT 
AAGTACCGGA ACCGGCAGCT GGAGCCGGAG TTTTACACCT CTCTCTTTCA TGACGTCGGG 
CTGAAGAGCT GCACCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ralgapb ( XM_012952781.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808235.1 Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X6, mRNA.

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CloneID	OXa35918
Clone ID Related Accession (Same CDS sequence)	XM_012952781.2
Accession Version	XM_012952781.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ral GTPase-activating protein subunit beta isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCATCTCGC GTCCCAGGGC CGACAGTGCT 
CCGCCGACCC CAGTGAACAG GCTAAGCATG CCTCAGAACG CCTCCACCAA CACTACGCCG 
CCGCACAGCC GAAGGCACCG CGCAGTCACT GTGAATAAGA CCACCGTAAA AACCAGCACG 
TCCGTCACAG CACATGGAGC AAAGGTGCAG CACCAACCGT CTTCCACCTC TCCCCTCTCC 
AGCCCCAACC AAACGAGCTC CGAGCCCCGA CCTCTCCCAG CCCCTATGAG GCCCAAAGTC 
AATAGCATCC TAAATCTGTT CGGTTCATGG CTGTTTGATG CAGCTTTGGT TCACTGCAGA 
CTGCAGAACG GAGTCAATCG AGACAGCAGC ATGACGGCAA TGACCACCCA GGCCGCCGTG 
GAGTTTCGGC GCAAAGGCTC CCAAATGTCT ACGGACACCA TGGTTTCCAG CACCGTGTTC 
GACGCGGCAG ACTTCCCGGA GAATTACGAG GCAGGAAGGG CCGAGGCCTG CGGGACCCTG 
TGTAGGATAT TCTGTAGCAA GAAAACGGGA GAGGAAATCC TGCCTGCCTA TCTGTCCAGG 
TTTTATATGG TCTTAGTCCA AGGTCTTCAG ATTGGAGACT TTATCTGCCA CCCCGTGCTA 
GCAAGCATCA TCCTGAACTC TCCGCCCCTT TTCTGCTGTG ACCTCAAGGG GATCAACGTT 
GTGGTTCCTT ACTTCATCTC TGCACTTGAA ACCATTTTGC CAGACAGGGA ACTTTCAAAG 
TTTAAAAACT ATGTGAATCC CATAGAACTC AGAAGATCCT CCATTCACAT TTTACTTTCC 
TTGCTGCCTC TGCCCCACCA CTTTGGCACC CTCAGGTCGG AGGTCCTACT GGAAGGAAAG 
TTTAGCAACG ATGACAGCTT GGGCAGCGAC AAACCGGTCA CCTTTCTTTC CCTGAAGCTG 
CGACTCGTCA ATATACTGAT AGGAGCGCTG CAAACTGAAA CGGATCCCAA CAACACACAA 
ATGATTCTAG GTGCCATGTT AAACATTGTC CAAGATTCAG CATTTCTGGA GGCAACTGGC 
AACCAGGTAG AAATGATTGA AAATAACCTA ATGAAAAGCC ACAGTCGGAC GAACAGCGGT 
ATCAGCACAG CAAGTGGCAA CAGCACAGAG CCGACCACTC CGGACAGCGA GAGACCGGCG 
CAGGCCTTGC TGAGAGACTA CGGATACCCA GCTCTTAATA CAGATACAGC GGCCGGCCTG 
CTCATGCGCA GCATCCACTT GGTCACCCAG AGACTCAACT CGCAGTGGAG GCCGGACATG 
AGCATCTCGC TGGCTGCCTT GGAACTGCTG TCCGGACTGG CCAAGGTAAA GGTGGGTATA 
GACTCTTCAG ACAGGAAGAG GGCAGTGAGT TCTGTGTGCA CCTACATCGT ATACCAGTGC 
TCCCGCCCGG CTCCGCATCA CTCCCGGGAT CTGCACTCCA TGATTGTAGC TGCCTTCCAG 
TGCCTGTGTG TGTGGCTTAC AGAGCATCCA GACATGTTAA ATGAAAAGGA TTGTCTAAAA 
GAGGTTCTAG AGATTGTAGA ACTGGGCATA TCCGGTAGCA AGTCAAAAAA TCCCGAAATG 
CAAGAGGTGC GTTACAAGGG GGATAAGGAG CCCAACCCAG CCTCTATGCG GGTGAAGGAC 
GCTGCAGAAG CAACACTTAC ATGTATCATG CAGCTGCTTG GGGCTTTCCC TCCGCCCAGC 
GGCCCGGCCT CGCCCTGCAG CTTGGTAAAT GAGATGACGC TCATCACATA CTCTAGGCTG 
CCCACGGTCA GCAAATACAG CTTCCGATAC TTTGTGCTGG ACAACAGTGT TATACTGGCC 
ATGCTGGAGC AGCCCCTGGG CAACGAGCAA AGTAACCCCA GTCCATCAAT CACAGTTCTG 
ATCCGAGGGA TGTCTGGCCG CCATGCATGG TCTATGCAGC TGTGTCACCT TCCAAGAGCT 
GCAAGAGCCA ATCAGAGGAT GTTTATCCCC GAGCCGAGGC CGGAGCCTAA TAACTACGTA 
GGCATCAAAT ACAACGTAAA GCATCGTCCT TTCCCAGAGG AGGTGGATAA GATTCCCTTT 
GTTAAGGCCG ATCTGAGCAT CCCTGATTTA CACGAGATTG TCAATGAAGA GTTGGAAGAA 
AGGCACGAAA AGTTGAAGCA CGGGATGGCC AAACAGATAG CTTATGAGGC CGAGCTGGAC 
CACCTGAAAG AGGAGGAGCT TAAAAAGAGA AGTTATCCTG ACCCTGTAAC GGAGTGCAAG 
CCTCCCCATC CGGCCCAGGA GTTCCAGACC GCCCGCCTGC TCCTCTCCCA CTTTGGCTTC 
CTCTCTCTTG AAGCACTTAA AGAACCTGCA AACAGCCGCC TACCTCCTCA CCTCATTGCA 
CTTGACTCAC ACATGCCAGG ATTCTTTGAA GATATTGGGT ACTTGGACCT ATTGCCCTGC 
CGCCCCTTCG ATACTGTTTT TATCTTCTAC ATGAAGGCTG GACAAAAAAC AGGTCAAGAG 
ATCTTGAAGA ATGTAGAGTC TTCCAAAAAC GTTCAGCCAC ATTTCCTGGA GTTCCTGCTG 
TCCTTGGGCT GGCCCGTGGA TGTCGGGAAG CACCCAGGCT GGACAGGCCA TGTTTCTACC 
AGTTGGTCCA TCAACTGCAG TGGTGAGAAT GAGCAACACG AGCAAGAGGA AGCACCTTCG 
GTAGATGACA CAGGAGGGAG CATATTTAAC GGGGAAAAGA AAGTTCTCTA CTATGCGGAT 
GCCCTGACTG AGATCGCCTT TGTTGTTCCG TCAGCTGTGG ATGCTCAAAG CGACTCTGTG 
GAAAATAACA CCTCGGAGCT TGACGGGGAC TCAAACATGG ATCTTCTCCC AGGGATACTG 
AAGCAGCCGC TCACCCTTGA GCTTTTCCCT ACACACGTTG AAAATGTCAG CTCAGCACAA 
AGGCATAGTC CCACGTCGCG CACTAAGAAG CTGCCCCAGG GGCGTGTAGT CCCACCTATG 
GGTCCCGAGA CCAAGGTAGC AGTTGTATGG CTGGAGCAGT ACGATGATTT AGAAAACTTC 
CCTCTACCAG ACCTCGCAGC AGAGACCAGC ACTGGTATAG AGACCACTAC AAACAGCAGC 
ACTTCTATCC GCAACAGTCT GCCTGAGAAA GAGTTCCCTG TGATTTTCAT CCACCCTTTG 
TACACCGGCC TCTTCCGAGT TAAAGTGCAC GGAGCCTCTG GGAAATTCAA CATGGTGATC 
CCTCTGGTAG ATGGAATGGT GGTGAGCAGA AGAGCACTTG GTTTCTTGGT CAGACAGACC 
GTCATCAACA TCTGCCGGCG GAAGCGACTG GAGAGCGACT CGTACAACAC CCCGCACGTG 
CGTCGGAAGC AGAAAATCAC CGACATCGTC AATAAGTACC GGAACCGGCA GCTGGAGCCG 
GAGTTTTACA CCTCTCTCTT TCATGACGTC GGGCTGAAGA GCTGCACCCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808235.1
CDS	195..4688
Translation	MYSEWRTLHLVLKNDQGHTSVLHSYPESVGRDVANAVVRPLGQALSAPSTAASESILKTDKEVKWTMEVICYGLTLPLEGDTVKYCVDVYTDWMMALVSHKDSIPQPVLREPNMYTQIILKHLQNVFVPRPDQMMSQIRLCLQVLRAVQKLARDSLLIARETWEVLLLFLLQINDTLLAAPTVAAGIADNLAEKLIGVLFEVWLLACTRCFPTPPYWKTAKEMVANWRHHPAVVEQWSKAICALTSRLLRFSYGPSFPPFKVPEEDASLIPGEMDNECVTQTWFRFLHMLSNPVDLSNPSIIGTTPKFQEQFLNGNGASQEINQYACLKQLPHIFFKAMRGVSCLVDAFLGISRPRADSAPPTPVNRLSMPQNASTNTTPPHSRRHRAVTVNKTTVKTSTSVTAHGAKVQHQPSSTSPLSSPNQTSSEPRPLPAPMRPKVNSILNLFGSWLFDAALVHCRLQNGVNRDSSMTAMTTQAAVEFRRKGSQMSTDTMVSSTVFDAADFPENYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMVLVQGLQIGDFICHPVLASIILNSPPLFCCDLKGINVVVPYFISALETILPDRELSKFKNYVNPIELRRSSIHILLSLLPLPHHFGTLRSEVLLEGKFSNDDSLGSDKPVTFLSLKLRLVNILIGALQTETDPNNTQMILGAMLNIVQDSAFLEATGNQVEMIENNLMKSHSRTNSGISTASGNSTEPTTPDSERPAQALLRDYGYPALNTDTAAGLLMRSIHLVTQRLNSQWRPDMSISLAALELLSGLAKVKVGIDSSDRKRAVSSVCTYIVYQCSRPAPHHSRDLHSMIVAAFQCLCVWLTEHPDMLNEKDCLKEVLEIVELGISGSKSKNPEMQEVRYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFPPPSGPASPCSLVNEMTLITYSRLPTVSKYSFRYFVLDNSVILAMLEQPLGNEQSNPSPSITVLIRGMSGRHAWSMQLCHLPRAARANQRMFIPEPRPEPNNYVGIKYNVKHRPFPEEVDKIPFVKADLSIPDLHEIVNEELEERHEKLKHGMAKQIAYEAELDHLKEEELKKRSYPDPVTECKPPHPAQEFQTARLLLSHFGFLSLEALKEPANSRLPPHLIALDSHMPGFFEDIGYLDLLPCRPFDTVFIFYMKAGQKTGQEILKNVESSKNVQPHFLEFLLSLGWPVDVGKHPGWTGHVSTSWSINCSGENEQHEQEEAPSVDDTGGSIFNGEKKVLYYADALTEIAFVVPSAVDAQSDSVENNTSELDGDSNMDLLPGILKQPLTLELFPTHVENVSSAQRHSPTSRTKKLPQGRVVPPMGPETKVAVVWLEQYDDLENFPLPDLAAETSTGIETTTNSSTSIRNSLPEKEFPVIFIHPLYTGLFRVKVHGASGKFNMVIPLVDGMVVSRRALGFLVRQTVINICRRKRLESDSYNTPHVRRKQKITDIVNKYRNRQLEPEFYTSLFHDVGLKSCTP

Target ORF information:

RefSeq Version	XM_012952781.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Ral GTPase activating protein, beta subunit (non-catalytic) (ralgapb), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952781.2

ORF Insert Sequence:

ATGTACTCTG AATGGCGGAC GCTGCACTTG GTCCTTAAGA ATGATCAGGG CCACACCAGC 
GTGTTGCACA GCTACCCAGA GAGCGTGGGC CGTGACGTGG CTAATGCAGT GGTGCGACCA 
CTGGGGCAGG CTCTCAGCGC CCCCTCCACA GCAGCAAGTG AAAGCATATT AAAAACAGAT 
AAAGAGGTGA AATGGACCAT GGAGGTGATT TGTTATGGGC TTACCCTGCC GCTGGAGGGG 
GACACCGTAA AATACTGTGT GGATGTTTAC ACAGATTGGA TGATGGCTCT TGTCAGCCAC 
AAGGACTCTA TTCCTCAGCC GGTTTTGAGA GAACCAAACA TGTATACCCA GATCATTCTC 
AAGCACCTCC AGAATGTCTT TGTGCCAAGG CCGGACCAGA TGATGAGCCA GATCCGCCTG 
TGTCTGCAGG TTCTGCGGGC TGTACAGAAG CTTGCCCGCG ATTCTCTCCT CATAGCCCGA 
GAGACATGGG AGGTCCTGCT TTTGTTCCTC CTCCAGATAA ACGACACCCT CCTCGCTGCT 
CCCACTGTGG CTGCGGGCAT AGCCGACAAC CTGGCTGAAA AGCTGATAGG GGTCCTTTTT 
GAGGTTTGGC TGTTGGCTTG CACTCGATGC TTTCCTACAC CTCCTTATTG GAAAACTGCC 
AAAGAAATGG TGGCTAACTG GCGTCACCAC CCGGCCGTGG TGGAACAGTG GAGTAAAGCT 
ATCTGTGCAC TGACGTCCAG GTTGCTAAGG TTTTCCTACG GCCCTTCGTT TCCTCCATTT 
AAAGTTCCAG AGGAAGATGC CAGTCTGATC CCAGGAGAGA TGGATAATGA ATGTGTCACC 
CAAACTTGGT TCCGGTTCCT TCACATGCTG AGCAATCCAG TGGATTTAAG CAACCCCAGT 
ATCATTGGCA CAACACCTAA ATTTCAGGAG CAGTTCCTGA ATGGCAATGG GGCTTCCCAG 
GAGATAAATC AGTACGCTTG CCTGAAGCAG CTGCCTCATA TCTTCTTCAA AGCGATGCGC 
GGGGTTAGTT GCCTTGTGGA TGCTTTCCTA GGCATCTCGC GTCCCAGGGC CGACAGTGCT 
CCGCCGACCC CAGTGAACAG GCTAAGCATG CCTCAGAACG CCTCCACCAA CACTACGCCG 
CCGCACAGCC GAAGGCACCG CGCAGTCACT GTGAATAAGA CCACCGTAAA AACCAGCACG 
TCCGTCACAG CACATGGAGC AAAGGTGCAG CACCAACCGT CTTCCACCTC TCCCCTCTCC 
AGCCCCAACC AAACGAGCTC CGAGCCCCGA CCTCTCCCAG CCCCTATGAG GCCCAAAGTC 
AATAGCATCC TAAATCTGTT CGGTTCATGG CTGTTTGATG CAGCTTTGGT TCACTGCAGA 
CTGCAGAACG GAGTCAATCG AGACAGCAGC ATGACGGCAA TGACCACCCA GGCCGCCGTG 
GAGTTTCGGC GCAAAGGCTC CCAAATGTCT ACGGACACCA TGGTTTCCAG CACCGTGTTC 
GACGCGGCAG ACTTCCCGGA GAATTACGAG GCAGGAAGGG CCGAGGCCTG CGGGACCCTG 
TGTAGGATAT TCTGTAGCAA GAAAACGGGA GAGGAAATCC TGCCTGCCTA TCTGTCCAGG 
TTTTATATGG TCTTAGTCCA AGGTCTTCAG ATTGGAGACT TTATCTGCCA CCCCGTGCTA 
GCAAGCATCA TCCTGAACTC TCCGCCCCTT TTCTGCTGTG ACCTCAAGGG GATCAACGTT 
GTGGTTCCTT ACTTCATCTC TGCACTTGAA ACCATTTTGC CAGACAGGGA ACTTTCAAAG 
TTTAAAAACT ATGTGAATCC CATAGAACTC AGAAGATCCT CCATTCACAT TTTACTTTCC 
TTGCTGCCTC TGCCCCACCA CTTTGGCACC CTCAGGTCGG AGGTCCTACT GGAAGGAAAG 
TTTAGCAACG ATGACAGCTT GGGCAGCGAC AAACCGGTCA CCTTTCTTTC CCTGAAGCTG 
CGACTCGTCA ATATACTGAT AGGAGCGCTG CAAACTGAAA CGGATCCCAA CAACACACAA 
ATGATTCTAG GTGCCATGTT AAACATTGTC CAAGATTCAG CATTTCTGGA GGCAACTGGC 
AACCAGGTAG AAATGATTGA AAATAACCTA ATGAAAAGCC ACAGTCGGAC GAACAGCGGT 
ATCAGCACAG CAAGTGGCAA CAGCACAGAG CCGACCACTC CGGACAGCGA GAGACCGGCG 
CAGGCCTTGC TGAGAGACTA CGGATACCCA GCTCTTAATA CAGATACAGC GGCCGGCCTG 
CTCATGCGCA GCATCCACTT GGTCACCCAG AGACTCAACT CGCAGTGGAG GCCGGACATG 
AGCATCTCGC TGGCTGCCTT GGAACTGCTG TCCGGACTGG CCAAGGTAAA GGTGGGTATA 
GACTCTTCAG ACAGGAAGAG GGCAGTGAGT TCTGTGTGCA CCTACATCGT ATACCAGTGC 
TCCCGCCCGG CTCCGCATCA CTCCCGGGAT CTGCACTCCA TGATTGTAGC TGCCTTCCAG 
TGCCTGTGTG TGTGGCTTAC AGAGCATCCA GACATGTTAA ATGAAAAGGA TTGTCTAAAA 
GAGGTTCTAG AGATTGTAGA ACTGGGCATA TCCGGTAGCA AGTCAAAAAA TCCCGAAATG 
CAAGAGGTGC GTTACAAGGG GGATAAGGAG CCCAACCCAG CCTCTATGCG GGTGAAGGAC 
GCTGCAGAAG CAACACTTAC ATGTATCATG CAGCTGCTTG GGGCTTTCCC TCCGCCCAGC 
GGCCCGGCCT CGCCCTGCAG CTTGGTAAAT GAGATGACGC TCATCACATA CTCTAGGCTG 
CCCACGGTCA GCAAATACAG CTTCCGATAC TTTGTGCTGG ACAACAGTGT TATACTGGCC 
ATGCTGGAGC AGCCCCTGGG CAACGAGCAA AGTAACCCCA GTCCATCAAT CACAGTTCTG 
ATCCGAGGGA TGTCTGGCCG CCATGCATGG TCTATGCAGC TGTGTCACCT TCCAAGAGCT 
GCAAGAGCCA ATCAGAGGAT GTTTATCCCC GAGCCGAGGC CGGAGCCTAA TAACTACGTA 
GGCATCAAAT ACAACGTAAA GCATCGTCCT TTCCCAGAGG AGGTGGATAA GATTCCCTTT 
GTTAAGGCCG ATCTGAGCAT CCCTGATTTA CACGAGATTG TCAATGAAGA GTTGGAAGAA 
AGGCACGAAA AGTTGAAGCA CGGGATGGCC AAACAGATAG CTTATGAGGC CGAGCTGGAC 
CACCTGAAAG AGGAGGAGCT TAAAAAGAGA AGTTATCCTG ACCCTGTAAC GGAGTGCAAG 
CCTCCCCATC CGGCCCAGGA GTTCCAGACC GCCCGCCTGC TCCTCTCCCA CTTTGGCTTC 
CTCTCTCTTG AAGCACTTAA AGAACCTGCA AACAGCCGCC TACCTCCTCA CCTCATTGCA 
CTTGACTCAC ACATGCCAGG ATTCTTTGAA GATATTGGGT ACTTGGACCT ATTGCCCTGC 
CGCCCCTTCG ATACTGTTTT TATCTTCTAC ATGAAGGCTG GACAAAAAAC AGGTCAAGAG 
ATCTTGAAGA ATGTAGAGTC TTCCAAAAAC GTTCAGCCAC ATTTCCTGGA GTTCCTGCTG 
TCCTTGGGCT GGCCCGTGGA TGTCGGGAAG CACCCAGGCT GGACAGGCCA TGTTTCTACC 
AGTTGGTCCA TCAACTGCAG TGGTGAGAAT GAGCAACACG AGCAAGAGGA AGCACCTTCG 
GTAGATGACA CAGGAGGGAG CATATTTAAC GGGGAAAAGA AAGTTCTCTA CTATGCGGAT 
GCCCTGACTG AGATCGCCTT TGTTGTTCCG TCAGCTGTGG ATGCTCAAAG CGACTCTGTG 
GAAAATAACA CCTCGGAGCT TGACGGGGAC TCAAACATGG ATCTTCTCCC AGGGATACTG 
AAGCAGCCGC TCACCCTTGA GCTTTTCCCT ACACACGTTG AAAATGTCAG CTCAGCACAA 
AGGCATAGTC CCACGTCGCG CACTAAGAAG CTGCCCCAGG GGCGTGTAGT CCCACCTATG 
GGTCCCGAGA CCAAGGTAGC AGTTGTATGG CTGGAGCAGT ACGATGATTT AGAAAACTTC 
CCTCTACCAG ACCTCGCAGC AGAGACCAGC ACTGGTATAG AGACCACTAC AAACAGCAGC 
ACTTCTATCC GCAACAGTCT GCCTGAGAAA GAGTTCCCTG TGATTTTCAT CCACCCTTTG 
TACACCGGCC TCTTCCGAGT TAAAGTGCAC GGAGCCTCTG GGAAATTCAA CATGGTGATC 
CCTCTGGTAG ATGGAATGGT GGTGAGCAGA AGAGCACTTG GTTTCTTGGT CAGACAGACC 
GTCATCAACA TCTGCCGGCG GAAGCGACTG GAGAGCGACT CGTACAACAC CCCGCACGTG 
CGTCGGAAGC AGAAAATCAC CGACATCGTC AATAAGTACC GGAACCGGCA GCTGGAGCCG 
GAGTTTTACA CCTCTCTCTT TCATGACGTC GGGCTGAAGA GCTGCACCCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ralgapb
Entrez Gene ID	100494268
Full Name	Ral GTPase activating protein, beta subunit (non-catalytic)
General protein information	Preferred Names Ral GTPase activating protein, beta subunit (non-catalytic) Names ral GTPase-activating protein subunit beta
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Pan troglodytes (chimpanzee)	RALGAPB	XP_003316982.1
Bos taurus (cattle)	RALGAPB	XP_003586895.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007925	XP_317557.4
	RALGAPB	XP_001094087.2
Mus musculus (house mouse)	Ralgapb	NP_808326.2
Gallus gallus (chicken)	RALGAPB	NP_001026017.1
Drosophila melanogaster (fruit fly)	CG34408	NP_572613.2
Homo sapiens (human)	RALGAPB	NP_065069.1
Canis lupus familiaris (dog)	RALGAPB	XP_005635111.1
Rattus norvegicus (Norway rat)	Ralgapb	NP_001258139.1
Danio rerio (zebrafish)	ralgapb	XP_002662611.2
Caenorhabditis elegans (roundworm)	D2085.5	NP_001022054.1
Xenopus tropicalis (tropical clawed frog)	ralgapb	XP_002932905.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ralgapb cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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