phkb gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012961646.2	XP_012817100.1	phosphorylase b kinase regulatory subunit beta isoform X1
XM_004914059.3	XP_004914116.1	phosphorylase b kinase regulatory subunit beta isoform X2
XM_012961646.3	XP_012817100.2	phosphorylase b kinase regulatory subunit beta isoform X1
XM_012961648.3	XP_012817102.2	phosphorylase b kinase regulatory subunit beta isoform X3
XM_002931669.3	XP_002931715.2	phosphorylase b kinase regulatory subunit beta isoform X1
XM_012961648.2	XP_012817102.1	phosphorylase b kinase regulatory subunit beta isoform X2
XM_002931669.4	XP_002931715.3	phosphorylase b kinase regulatory subunit beta isoform X1
XM_018093360.1	XP_017948849.1	phosphorylase b kinase regulatory subunit beta isoform X4
XM_012961645.2	XP_012817099.1	phosphorylase b kinase regulatory subunit beta isoform X1
XM_004914060.3	XP_004914117.1	phosphorylase b kinase regulatory subunit beta isoform X3
XM_012961645.3	XP_012817099.2	phosphorylase b kinase regulatory subunit beta isoform X1
XM_031900821.1	XP_031756681.1	phosphorylase b kinase regulatory subunit beta isoform X2
XM_004914059.4	XP_004914116.2	phosphorylase b kinase regulatory subunit beta isoform X3
XM_004914060.4	XP_004914117.2	phosphorylase b kinase regulatory subunit beta isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa44031	XM_018093360.1 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa56949	XM_012961645.3 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa56949	XM_012961646.3 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa56949	XM_002931669.4 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa56950	XM_031900821.1 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa56951	XM_004914059.4 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa56951	XM_012961648.3 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa56952	XM_004914060.4 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa09606	XM_012961645.2 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa09606	XM_002931669.3 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa09606	XM_012961646.2 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa09607	XM_004914059.3 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa09607	XM_012961648.2 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa09608	XM_004914060.3 Latest version!	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

phkb ( XM_018093360.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948849.1 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa44031
Clone ID Related Accession (Same CDS sequence)	XM_018093360.1
Accession Version	XM_018093360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGG TCCTGGCCGA TGTTAGCTGG AAGGTGCTCG AGTTAAGGGC CAGATCTAAA 
CGCTCAGTGA AAATCACAGT GTTGAATTAT CAGAGTCCAC AAACTGGTTT GTTCCCGACC 
AAAACTGCTG GTGACTTTAA GAACGCGAAG ATCCATGACA GTCTATACTG TGCTGCCAGC 
GCCTGGGCCC TCGCTCTTGC ATACAGGCGC ATTGATGATG ACAAGGGAAG AACCCATGAG 
CTGGAGCATT CTGCTATAAA ATGTATGAGA GGAATTCTGT ACTGTTACAT GCGGCAGGCT 
GATAAGGTTG AGCAGTTTAA ACAGGATCCA AGTCCACAAA AATGCCTTCA TTCTATATTC 
AATATAGACA ATGGAGATGA GATATTACCC TATAACGATT ATGGACATAT ACAGATAAAC 
GCAGTGTCAC TTTTCCTTTT GTATCTTGTT GAGATGATAT CATCTGGACT ACAGATAATC 
TACAATACAG ATGAGGTGTC GTTCATTCAG AACCTTGTAT TCTGTGTAGA AAGAGCATAC 
CGTGTTCCTG ACTTTGGAAT CTGGGAAAGG GGCAGCAAGT ATAACAATGG CAGCACAGAA 
TTACATTCCA GTTCTGTTGG TTTGGCTAAA GCTGCCCTTG AAGCCATTGA TGGATTCAAC 
CTTTTTGGAA ACCAGGGCTG CTCTTGGTCT GTCATCTTTG TGGATCTTGA TGCTCATAAC 
AGGAACAGGC AAACTCTGAG TTCATTGCTT CCCAGAGAGT CCCGATCCCA TAACACAGAT 
GCAGCCCTCC TTACCTGTAT CAGTTACCCT GCATTTGCTG TTGATGATGA TGCTCTGTTT 
AACCAAACAC TGGATAAAAT AGAACGTAAG CTTAAAGGAA AATATGGCTT TAAGCGATTT 
CTAAGGGATG GTTATCGGAC TGCACTAGAA GATGAAAACA GACGGTACTA CAAACCTGCT 
GAAATCAAGC TATTTGACGG AATTGAATGT GAATTCCCTT TGTTTTTTAT CTACATGATC 
ATTGATGGTG TGTTCAGAGG GAAAGCTGAG CAAGTGAAAG AATATGAAGC ACTCTTAGAT 
CCTGTGCTAT TTTATGGACT TGATGGCTGT CCCGTGGTTC CAAAATATTA CTATGTGCCT 
GCTGATTTTG TTGAAGCAGA GAAGATGAAC CCAAACAGCC AAAAGAGATT TCCTAGCAAC 
TGTGGGAGAG ATGGAAAACT CTTTCTCTGG GGACAAGCTC TGTACATTAT TGCTAAACTG 
CTGGCCGAAG AATTAATAAG TCCAAAAGAC ATTGATCCAA TGAACCGTTT TGTTCATCTT 
CGGGATCAGC GAAACATCAG AATGCGGTAC TCTAACCAGG GCCCCTTAGA AAATGATGCC 
ATGATACATG TGGCACTGAT AGCTGAAAGC CAACGATTGC AGGTTTTTCT CAACACTTAT 
GGCATCCAAA CCCAGACCCC ACAGCAAGTG GAACCTATTC AGATATGGCC TCAGCAAGAA 
CTTGTGAAGG CTTATCTTCA CTTAGGAGTA AATAACAAGT TGGGGCTTTC TGGAAGGCCT 
GACAGGCCAA TTGGATGCCT TGGCACATCA AAGATTTATC GTATTCTTGG GAAGACCGTT 
GTTTCCTACC CTATTGTCTT TGATTTAAGT GACTTTTACA TGTCACAGGA TGTGATGCTT 
CTGATCGATG ACATACAGAA TGCTTTGCAG TTCATTAGGC AGTATTGGAA AATGAATGGA 
CGTCCCTTGT TTTTGGTTCT CATTCGGGAA GATAACATCA GGGGAAGTCG ATTTAATCCA 
ATCTTAGACA TGTTGGCTTC CTTTAAGAAT GGAAATGTGG CCGGTGTGAA GGTTCATGTG 
GACAGACTGC AGACCTTAAT ATCCGGTGCA GTGGTTGAGC AGCTGGATTT CTTGAGGATC 
AATGAGACAG AGGAGGTGCC TGAATTCAAA AGTTTTGAAG AGCTTGAAGT ACCGAAACAT 
TCAAAGGTTA ACAGGCAAGC AAGCATCCCT ATTGCAGATA TGGAGCATCA GCCAGAAATA 
AACATGGGAG AATGGAAACT CAAACCAACC TATGAGGTTC TCGAGAAACT AAATGACTGC 
ACCTGCCTGG CAAGCCAGGC CATCTTACTA AGTATCTTAC TGAAGAGAGA AGGACCTCAT 
TTTATAACCA AGGAAGGTAC TGTCTCTGAT TTTATGGCAA GAATCTATAG AAAAGCTGGA 
AGCAATAAAC TGTGGTCGGT TGTACGTCTT GCTGCCAGTC TTTTAGGTAA AATAGTGGAC 
AGCCTTGCAC CCTCTATAAC TAATGTTTTA GTTCAGGGCA AACAGGTAAC ACTGGGAGTC 
TTTGGCCACG AGGAAGCGGT TATTTCCAAT CCCTTGTCTC CAGGAGTGAT CAAGGATATC 
CTCTACAAAA AGTGCAGCCC CTATGATGAG CGAGAGGCTG TGCTGCAACA AGAGCTAGTT 
ATTCACATTG GCTGGATTAT CTCCAACACA CCAGAGCTTT TCAGCGGAAT GTTAAAGATA 
CGGGTCGGAT GGATTGTGCA TGCAATGAGG TATGAACTGA AGATCCGCAG TGGAGATCAG 
CTAGAAAGGG ACATCTACCA GCTGTCTCCA GGAGAAATCA AAGAGCTTCT CCTGTACATA 
CTTCAGCCAC AGCAGCAAGG AAGATGTTGG CTTCATCGAA GGCAGTTAGA TGGATCCCTA 
AACCGAAACC CATCTGGCTT CTACGACAGA GTCTGGCAGA TCCTGGAGAG GACACCCAGT 
GGTATCATAG TTGCTGGCAA ATTTCTACCC CAACAACCAA CTCTGTCTGA CATGACAATG 
TATGAGATGA ACTTCTCCCT TCTGGTAGAA GACATGTTAC AGAACATCGC CCAGCCTGAA 
TATCGACAAA TTGTTGTTGA GCTGCTAATG GTTGTTTCTG TTATCCTGGA GAGAAACCCG 
GAGTTTGAAT TTCAAGATAA AGTTGATTTA GACAAACTAG TCAAAGAGGC ATTTAATGAT 
TTCCAGCAAG ACCAGAGCAG ACTTCAGGGA GCGGAGAAGC AGGATGACAT GACGATGTTT 
TACAATACTC CCCCACTGGG CAAGCGAGGG ACCTGCAGCT ACCTGACAAA AGCAGTGATG 
AATATGCTGC TGGAAGCTGA AGTCAAGCCA AGTACTGAGG ACCCTTGCAT TGTTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948849.1
CDS	57..3236
Translation	MAQVLADVSWKVLELRARSKRSVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILPYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_018093360.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093360.1

ORF Insert Sequence:

ATGGCTCAGG TCCTGGCCGA TGTTAGCTGG AAGGTGCTCG AGTTAAGGGC CAGATCTAAA 
CGCTCAGTGA AAATCACAGT GTTGAATTAT CAGAGTCCAC AAACTGGTTT GTTCCCGACC 
AAAACTGCTG GTGACTTTAA GAACGCGAAG ATCCATGACA GTCTATACTG TGCTGCCAGC 
GCCTGGGCCC TCGCTCTTGC ATACAGGCGC ATTGATGATG ACAAGGGAAG AACCCATGAG 
CTGGAGCATT CTGCTATAAA ATGTATGAGA GGAATTCTGT ACTGTTACAT GCGGCAGGCT 
GATAAGGTTG AGCAGTTTAA ACAGGATCCA AGTCCACAAA AATGCCTTCA TTCTATATTC 
AATATAGACA ATGGAGATGA GATATTACCC TATAACGATT ATGGACATAT ACAGATAAAC 
GCAGTGTCAC TTTTCCTTTT GTATCTTGTT GAGATGATAT CATCTGGACT ACAGATAATC 
TACAATACAG ATGAGGTGTC GTTCATTCAG AACCTTGTAT TCTGTGTAGA AAGAGCATAC 
CGTGTTCCTG ACTTTGGAAT CTGGGAAAGG GGCAGCAAGT ATAACAATGG CAGCACAGAA 
TTACATTCCA GTTCTGTTGG TTTGGCTAAA GCTGCCCTTG AAGCCATTGA TGGATTCAAC 
CTTTTTGGAA ACCAGGGCTG CTCTTGGTCT GTCATCTTTG TGGATCTTGA TGCTCATAAC 
AGGAACAGGC AAACTCTGAG TTCATTGCTT CCCAGAGAGT CCCGATCCCA TAACACAGAT 
GCAGCCCTCC TTACCTGTAT CAGTTACCCT GCATTTGCTG TTGATGATGA TGCTCTGTTT 
AACCAAACAC TGGATAAAAT AGAACGTAAG CTTAAAGGAA AATATGGCTT TAAGCGATTT 
CTAAGGGATG GTTATCGGAC TGCACTAGAA GATGAAAACA GACGGTACTA CAAACCTGCT 
GAAATCAAGC TATTTGACGG AATTGAATGT GAATTCCCTT TGTTTTTTAT CTACATGATC 
ATTGATGGTG TGTTCAGAGG GAAAGCTGAG CAAGTGAAAG AATATGAAGC ACTCTTAGAT 
CCTGTGCTAT TTTATGGACT TGATGGCTGT CCCGTGGTTC CAAAATATTA CTATGTGCCT 
GCTGATTTTG TTGAAGCAGA GAAGATGAAC CCAAACAGCC AAAAGAGATT TCCTAGCAAC 
TGTGGGAGAG ATGGAAAACT CTTTCTCTGG GGACAAGCTC TGTACATTAT TGCTAAACTG 
CTGGCCGAAG AATTAATAAG TCCAAAAGAC ATTGATCCAA TGAACCGTTT TGTTCATCTT 
CGGGATCAGC GAAACATCAG AATGCGGTAC TCTAACCAGG GCCCCTTAGA AAATGATGCC 
ATGATACATG TGGCACTGAT AGCTGAAAGC CAACGATTGC AGGTTTTTCT CAACACTTAT 
GGCATCCAAA CCCAGACCCC ACAGCAAGTG GAACCTATTC AGATATGGCC TCAGCAAGAA 
CTTGTGAAGG CTTATCTTCA CTTAGGAGTA AATAACAAGT TGGGGCTTTC TGGAAGGCCT 
GACAGGCCAA TTGGATGCCT TGGCACATCA AAGATTTATC GTATTCTTGG GAAGACCGTT 
GTTTCCTACC CTATTGTCTT TGATTTAAGT GACTTTTACA TGTCACAGGA TGTGATGCTT 
CTGATCGATG ACATACAGAA TGCTTTGCAG TTCATTAGGC AGTATTGGAA AATGAATGGA 
CGTCCCTTGT TTTTGGTTCT CATTCGGGAA GATAACATCA GGGGAAGTCG ATTTAATCCA 
ATCTTAGACA TGTTGGCTTC CTTTAAGAAT GGAAATGTGG CCGGTGTGAA GGTTCATGTG 
GACAGACTGC AGACCTTAAT ATCCGGTGCA GTGGTTGAGC AGCTGGATTT CTTGAGGATC 
AATGAGACAG AGGAGGTGCC TGAATTCAAA AGTTTTGAAG AGCTTGAAGT ACCGAAACAT 
TCAAAGGTTA ACAGGCAAGC AAGCATCCCT ATTGCAGATA TGGAGCATCA GCCAGAAATA 
AACATGGGAG AATGGAAACT CAAACCAACC TATGAGGTTC TCGAGAAACT AAATGACTGC 
ACCTGCCTGG CAAGCCAGGC CATCTTACTA AGTATCTTAC TGAAGAGAGA AGGACCTCAT 
TTTATAACCA AGGAAGGTAC TGTCTCTGAT TTTATGGCAA GAATCTATAG AAAAGCTGGA 
AGCAATAAAC TGTGGTCGGT TGTACGTCTT GCTGCCAGTC TTTTAGGTAA AATAGTGGAC 
AGCCTTGCAC CCTCTATAAC TAATGTTTTA GTTCAGGGCA AACAGGTAAC ACTGGGAGTC 
TTTGGCCACG AGGAAGCGGT TATTTCCAAT CCCTTGTCTC CAGGAGTGAT CAAGGATATC 
CTCTACAAAA AGTGCAGCCC CTATGATGAG CGAGAGGCTG TGCTGCAACA AGAGCTAGTT 
ATTCACATTG GCTGGATTAT CTCCAACACA CCAGAGCTTT TCAGCGGAAT GTTAAAGATA 
CGGGTCGGAT GGATTGTGCA TGCAATGAGG TATGAACTGA AGATCCGCAG TGGAGATCAG 
CTAGAAAGGG ACATCTACCA GCTGTCTCCA GGAGAAATCA AAGAGCTTCT CCTGTACATA 
CTTCAGCCAC AGCAGCAAGG AAGATGTTGG CTTCATCGAA GGCAGTTAGA TGGATCCCTA 
AACCGAAACC CATCTGGCTT CTACGACAGA GTCTGGCAGA TCCTGGAGAG GACACCCAGT 
GGTATCATAG TTGCTGGCAA ATTTCTACCC CAACAACCAA CTCTGTCTGA CATGACAATG 
TATGAGATGA ACTTCTCCCT TCTGGTAGAA GACATGTTAC AGAACATCGC CCAGCCTGAA 
TATCGACAAA TTGTTGTTGA GCTGCTAATG GTTGTTTCTG TTATCCTGGA GAGAAACCCG 
GAGTTTGAAT TTCAAGATAA AGTTGATTTA GACAAACTAG TCAAAGAGGC ATTTAATGAT 
TTCCAGCAAG ACCAGAGCAG ACTTCAGGGA GCGGAGAAGC AGGATGACAT GACGATGTTT 
TACAATACTC CCCCACTGGG CAAGCGAGGG ACCTGCAGCT ACCTGACAAA AGCAGTGATG 
AATATGCTGC TGGAAGCTGA AGTCAAGCCA AGTACTGAGG ACCCTTGCAT TGTTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_012961646.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817100.2 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa56949
Clone ID Related Accession (Same CDS sequence)	XM_012961646.3 , XM_002931669.4 , XM_012961645.3
Accession Version	XM_012961646.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961646.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTATCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAACT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGATGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGCGAGAT 
GGAAAACTCT TCCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTCGGTTG TACGTCTTGC TGCCAGTCTT TTAGGTAAAA TAGTGGACAG CCTTGCACCC 
TCTATAACTA ATGTTTTAGT TCAGGGCAAA CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817100.2
CDS	111..3398
Translation	MQPIEMANSVDKAVSFSPTFLRSGKESYGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILSYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_012961646.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961646.3

ORF Insert Sequence:

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTATCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAACT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGATGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGCGAGAT 
GGAAAACTCT TCCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTCGGTTG TACGTCTTGC TGCCAGTCTT TTAGGTAAAA TAGTGGACAG CCTTGCACCC 
TCTATAACTA ATGTTTTAGT TCAGGGCAAA CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_002931669.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931715.3 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X1, mRNA.

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CloneID	OXa56949
Clone ID Related Accession (Same CDS sequence)	XM_012961646.3 , XM_002931669.4 , XM_012961645.3
Accession Version	XM_002931669.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931669.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTATCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAACT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGATGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGCGAGAT 
GGAAAACTCT TCCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTCGGTTG TACGTCTTGC TGCCAGTCTT TTAGGTAAAA TAGTGGACAG CCTTGCACCC 
TCTATAACTA ATGTTTTAGT TCAGGGCAAA CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931715.3
CDS	183..3470
Translation	MQPIEMANSVDKAVSFSPTFLRSGKESYGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILSYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_002931669.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931669.4

ORF Insert Sequence:

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTATCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAACT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGATGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGCGAGAT 
GGAAAACTCT TCCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTCGGTTG TACGTCTTGC TGCCAGTCTT TTAGGTAAAA TAGTGGACAG CCTTGCACCC 
TCTATAACTA ATGTTTTAGT TCAGGGCAAA CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_012961645.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817099.2 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa56949
Clone ID Related Accession (Same CDS sequence)	XM_012961646.3 , XM_002931669.4 , XM_012961645.3
Accession Version	XM_012961645.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961645.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTATCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAACT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGATGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGCGAGAT 
GGAAAACTCT TCCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTCGGTTG TACGTCTTGC TGCCAGTCTT TTAGGTAAAA TAGTGGACAG CCTTGCACCC 
TCTATAACTA ATGTTTTAGT TCAGGGCAAA CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817099.2
CDS	109..3396
Translation	MQPIEMANSVDKAVSFSPTFLRSGKESYGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILSYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_012961645.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961645.3

ORF Insert Sequence:

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTATCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAACT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGATGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGCGAGAT 
GGAAAACTCT TCCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTCGGTTG TACGTCTTGC TGCCAGTCTT TTAGGTAAAA TAGTGGACAG CCTTGCACCC 
TCTATAACTA ATGTTTTAGT TCAGGGCAAA CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_031900821.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031756681.1 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X4, mRNA.

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CloneID	OXa56950
Clone ID Related Accession (Same CDS sequence)	XM_031900821.1
Accession Version	XM_031900821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTATCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAACT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGATGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGCGAGAT 
GGAAAACTCT TCCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTTTGTGG TTCGTTACAC TGCGGCCTTC ACACAGAAAT TAGCTTCCTC TATAGCACCA 
CAAATTACCA CCATACTGGT ACATGGGAAG CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031756681.1
CDS	183..3470
Translation	MQPIEMANSVDKAVSFSPTFLRSGKESYGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILSYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWFVVRYTAAFTQKLASSIAPQITTILVHGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_031900821.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031900821.1

ORF Insert Sequence:

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTATCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAACT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGATGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGCGAGAT 
GGAAAACTCT TCCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTTTGTGG TTCGTTACAC TGCGGCCTTC ACACAGAAAT TAGCTTCCTC TATAGCACCA 
CAAATTACCA CCATACTGGT ACATGGGAAG CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_012961648.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817102.2 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X6, mRNA.

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CloneID	OXa56951
Clone ID Related Accession (Same CDS sequence)	XM_012961648.3 , XM_004914059.4
Accession Version	XM_012961648.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012961648.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GCTCCGTGTA TGAACCAATT AAGAGCTCGG ACCTACCCAG GCCAGACCAT 
GAAAGCCTGT GGGAAAAATT AGATCGGTAT TATAGGATTG TGAAAATCAC AGTGTTGAAT 
TATCAGAGTC CACAAACTGG TTTGTTCCCG ACCAAAACTG CTGGTGACTT TAAGAACGCG 
AAGATCCATG ACAGTCTATA CTGTGCTGCC AGCGCCTGGG CCCTCGCTCT TGCATACAGG 
CGCATTGATG ATGACAAGGG AAGAACCCAT GAGCTGGAGC ATTCTGCTAT AAAATGTATG 
AGAGGAATTC TGTACTGTTA CATGCGGCAG GCTGATAAGG TTGAGCAGTT TAAACAGGAT 
CCAAGTCCAC AAAAATGCCT TCATTCTATA TTCAATATAG ACAATGGAGA TGAGATATTA 
TCCTATAACG ATTATGGACA TATACAGATA AACGCAGTGT CACTTTTCCT TTTGTATCTT 
GTTGAGATGA TATCATCTGG ACTACAGATA ATCTACAATA CAGATGAGGT GTCGTTCATT 
CAGAACCTTG TATTCTGTGT AGAAAGAGCA TACCGTGTTC CTGACTTTGG AATCTGGGAA 
AGGGGCAGCA AGTATAACAA TGGCAGCACA GAACTACATT CCAGTTCTGT TGGTTTGGCT 
AAAGCTGCCC TTGAAGCCAT TGATGGATTC AACCTTTTTG GAAACCAGGG CTGCTCTTGG 
TCTGTCATCT TTGTGGATCT TGATGCTCAT AACAGGAACA GGCAAACTCT GAGTTCATTG 
CTTCCCAGAG AGTCCCGATC CCATAACACA GATGCAGCCC TCCTTACCTG TATCAGTTAC 
CCTGCATTTG CTGTTGATGA TGATGCTCTG TTTAACCAAA CACTGGATAA AATAGAACGT 
AAGCTTAAAG GAAAATATGG CTTTAAGCGA TTTCTAAGGG ATGGTTATCG GACTGCACTA 
GAAGATGAAA ACAGACGGTA CTACAAACCT GCTGAAATCA AGCTATTTGA TGGAATTGAA 
TGTGAATTCC CTTTGTTTTT TATCTACATG ATCATTGATG GTGTGTTCAG AGGGAAAGCT 
GAGCAAGTGA AAGAATATGA AGCACTCTTA GATCCTGTGC TATTTTATGG ACTTGATGGC 
TGTCCCGTGG TTCCAAAATA TTACTATGTG CCTGCTGATT TTGTTGAAGC AGAGAAGATG 
AACCCAAACA GCCAAAAGAG ATTTCCTAGC AACTGTGGGC GAGATGGAAA ACTCTTCCTC 
TGGGGACAAG CTCTGTACAT TATTGCTAAA CTGCTGGCCG AAGAATTAAT AAGTCCAAAA 
GACATTGATC CAATGAACCG TTTTGTTCAT CTTCGGGATC AGCGAAACAT CAGAATGCGG 
TACTCTAACC AGGGCCCCTT AGAAAATGAT GCCATGATAC ATGTGGCACT GATAGCTGAA 
AGCCAACGAT TGCAGGTTTT TCTCAACACT TATGGCATCC AAACCCAGAC CCCACAGCAA 
GTGGAACCTA TTCAGATATG GCCTCAGCAA GAACTTGTGA AGGCTTATCT TCACTTAGGA 
GTAAATAACA AGTTGGGGCT TTCTGGAAGG CCTGACAGGC CAATTGGATG CCTTGGCACA 
TCAAAGATTT ATCGTATTCT TGGGAAGACC GTTGTTTCCT ACCCTATTGT CTTTGATTTA 
AGTGACTTTT ACATGTCACA GGATGTGATG CTTCTGATCG ATGACATACA GAATGCTTTG 
CAGTTCATTA GGCAGTATTG GAAAATGAAT GGACGTCCCT TGTTTTTGGT TCTCATTCGG 
GAAGATAACA TCAGGGGAAG TCGATTTAAT CCAATCTTAG ACATGTTGGC TTCCTTTAAG 
AATGGAAATG TGGCCGGTGT GAAGGTTCAT GTGGACAGAC TGCAGACCTT AATATCCGGT 
GCAGTGGTTG AGCAGCTGGA TTTCTTGAGG ATCAATGAGA CAGAGGAGGT GCCTGAATTC 
AAAAGTTTTG AAGAGCTTGA AGTACCGAAA CATTCAAAGG TTAACAGGCA AGCAAGCATC 
CCTATTGCAG ATATGGAGCA TCAGCCAGAA ATAAACATGG GAGAATGGAA ACTCAAACCA 
ACCTATGAGG TTCTCGAGAA ACTAAATGAC TGCACCTGCC TGGCAAGCCA GGCCATCTTA 
CTAAGTATCT TACTGAAGAG AGAAGGACCT CATTTTATAA CCAAGGAAGG TACTGTCTCT 
GATTTTATGG CAAGAATCTA TAGAAAAGCT GGAAGCAATA AACTGTGGTC GGTTGTACGT 
CTTGCTGCCA GTCTTTTAGG TAAAATAGTG GACAGCCTTG CACCCTCTAT AACTAATGTT 
TTAGTTCAGG GCAAACAGGT AACACTGGGA GTCTTTGGCC ACGAGGAAGC GGTTATTTCC 
AATCCCTTGT CTCCAGGAGT GATCAAGGAT ATCCTCTACA AAAAGTGCAG CCCCTATGAT 
GAGCGAGAGG CTGTGCTGCA ACAAGAGCTA GTTATTCACA TTGGCTGGAT TATCTCCAAC 
ACACCAGAGC TTTTCAGCGG AATGTTAAAG ATACGGGTCG GATGGATTGT GCATGCAATG 
AGGTATGAAC TGAAGATCCG CAGTGGAGAT CAGCTAGAAA GGGACATCTA CCAGCTGTCT 
CCAGGAGAAA TCAAAGAGCT TCTCCTGTAC ATACTTCAGC CACAGCAGCA AGGAAGATGT 
TGGCTTCATC GAAGGCAGTT AGATGGATCC CTAAACCGAA ACCCATCTGG CTTCTACGAC 
AGAGTCTGGC AGATCCTGGA GAGGACACCC AGTGGTATCA TAGTTGCTGG CAAATTTCTA 
CCCCAACAAC CAACTCTGTC TGACATGACA ATGTATGAGA TGAACTTCTC CCTTCTGGTA 
GAAGACATGT TACAGAACAT CGCCCAGCCT GAATATCGAC AAATTGTTGT TGAGCTGCTA 
ATGGTTGTTT CTGTTATCCT GGAGAGAAAC CCGGAGTTTG AATTTCAAGA TAAAGTTGAT 
TTAGACAAAC TAGTCAAAGA GGCATTTAAT GATTTCCAGC AAGACCAGAG CAGACTTCAG 
GGAGCGGAGA AGCAGGATGA CATGACGATG TTTTACAATA CTCCCCCACT GGGCAAGCGA 
GGGACCTGCA GCTACCTGAC AAAAGCAGTG ATGAATATGC TGCTGGAAGC TGAAGTCAAG 
CCAAGTACTG AGGACCCTTG CATTGTTAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817102.2
CDS	108..3380
Translation	MQPIEMANSVDKAVSFSPTFLRSGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILSYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_012961648.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961648.3

ORF Insert Sequence:

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GCTCCGTGTA TGAACCAATT AAGAGCTCGG ACCTACCCAG GCCAGACCAT 
GAAAGCCTGT GGGAAAAATT AGATCGGTAT TATAGGATTG TGAAAATCAC AGTGTTGAAT 
TATCAGAGTC CACAAACTGG TTTGTTCCCG ACCAAAACTG CTGGTGACTT TAAGAACGCG 
AAGATCCATG ACAGTCTATA CTGTGCTGCC AGCGCCTGGG CCCTCGCTCT TGCATACAGG 
CGCATTGATG ATGACAAGGG AAGAACCCAT GAGCTGGAGC ATTCTGCTAT AAAATGTATG 
AGAGGAATTC TGTACTGTTA CATGCGGCAG GCTGATAAGG TTGAGCAGTT TAAACAGGAT 
CCAAGTCCAC AAAAATGCCT TCATTCTATA TTCAATATAG ACAATGGAGA TGAGATATTA 
TCCTATAACG ATTATGGACA TATACAGATA AACGCAGTGT CACTTTTCCT TTTGTATCTT 
GTTGAGATGA TATCATCTGG ACTACAGATA ATCTACAATA CAGATGAGGT GTCGTTCATT 
CAGAACCTTG TATTCTGTGT AGAAAGAGCA TACCGTGTTC CTGACTTTGG AATCTGGGAA 
AGGGGCAGCA AGTATAACAA TGGCAGCACA GAACTACATT CCAGTTCTGT TGGTTTGGCT 
AAAGCTGCCC TTGAAGCCAT TGATGGATTC AACCTTTTTG GAAACCAGGG CTGCTCTTGG 
TCTGTCATCT TTGTGGATCT TGATGCTCAT AACAGGAACA GGCAAACTCT GAGTTCATTG 
CTTCCCAGAG AGTCCCGATC CCATAACACA GATGCAGCCC TCCTTACCTG TATCAGTTAC 
CCTGCATTTG CTGTTGATGA TGATGCTCTG TTTAACCAAA CACTGGATAA AATAGAACGT 
AAGCTTAAAG GAAAATATGG CTTTAAGCGA TTTCTAAGGG ATGGTTATCG GACTGCACTA 
GAAGATGAAA ACAGACGGTA CTACAAACCT GCTGAAATCA AGCTATTTGA TGGAATTGAA 
TGTGAATTCC CTTTGTTTTT TATCTACATG ATCATTGATG GTGTGTTCAG AGGGAAAGCT 
GAGCAAGTGA AAGAATATGA AGCACTCTTA GATCCTGTGC TATTTTATGG ACTTGATGGC 
TGTCCCGTGG TTCCAAAATA TTACTATGTG CCTGCTGATT TTGTTGAAGC AGAGAAGATG 
AACCCAAACA GCCAAAAGAG ATTTCCTAGC AACTGTGGGC GAGATGGAAA ACTCTTCCTC 
TGGGGACAAG CTCTGTACAT TATTGCTAAA CTGCTGGCCG AAGAATTAAT AAGTCCAAAA 
GACATTGATC CAATGAACCG TTTTGTTCAT CTTCGGGATC AGCGAAACAT CAGAATGCGG 
TACTCTAACC AGGGCCCCTT AGAAAATGAT GCCATGATAC ATGTGGCACT GATAGCTGAA 
AGCCAACGAT TGCAGGTTTT TCTCAACACT TATGGCATCC AAACCCAGAC CCCACAGCAA 
GTGGAACCTA TTCAGATATG GCCTCAGCAA GAACTTGTGA AGGCTTATCT TCACTTAGGA 
GTAAATAACA AGTTGGGGCT TTCTGGAAGG CCTGACAGGC CAATTGGATG CCTTGGCACA 
TCAAAGATTT ATCGTATTCT TGGGAAGACC GTTGTTTCCT ACCCTATTGT CTTTGATTTA 
AGTGACTTTT ACATGTCACA GGATGTGATG CTTCTGATCG ATGACATACA GAATGCTTTG 
CAGTTCATTA GGCAGTATTG GAAAATGAAT GGACGTCCCT TGTTTTTGGT TCTCATTCGG 
GAAGATAACA TCAGGGGAAG TCGATTTAAT CCAATCTTAG ACATGTTGGC TTCCTTTAAG 
AATGGAAATG TGGCCGGTGT GAAGGTTCAT GTGGACAGAC TGCAGACCTT AATATCCGGT 
GCAGTGGTTG AGCAGCTGGA TTTCTTGAGG ATCAATGAGA CAGAGGAGGT GCCTGAATTC 
AAAAGTTTTG AAGAGCTTGA AGTACCGAAA CATTCAAAGG TTAACAGGCA AGCAAGCATC 
CCTATTGCAG ATATGGAGCA TCAGCCAGAA ATAAACATGG GAGAATGGAA ACTCAAACCA 
ACCTATGAGG TTCTCGAGAA ACTAAATGAC TGCACCTGCC TGGCAAGCCA GGCCATCTTA 
CTAAGTATCT TACTGAAGAG AGAAGGACCT CATTTTATAA CCAAGGAAGG TACTGTCTCT 
GATTTTATGG CAAGAATCTA TAGAAAAGCT GGAAGCAATA AACTGTGGTC GGTTGTACGT 
CTTGCTGCCA GTCTTTTAGG TAAAATAGTG GACAGCCTTG CACCCTCTAT AACTAATGTT 
TTAGTTCAGG GCAAACAGGT AACACTGGGA GTCTTTGGCC ACGAGGAAGC GGTTATTTCC 
AATCCCTTGT CTCCAGGAGT GATCAAGGAT ATCCTCTACA AAAAGTGCAG CCCCTATGAT 
GAGCGAGAGG CTGTGCTGCA ACAAGAGCTA GTTATTCACA TTGGCTGGAT TATCTCCAAC 
ACACCAGAGC TTTTCAGCGG AATGTTAAAG ATACGGGTCG GATGGATTGT GCATGCAATG 
AGGTATGAAC TGAAGATCCG CAGTGGAGAT CAGCTAGAAA GGGACATCTA CCAGCTGTCT 
CCAGGAGAAA TCAAAGAGCT TCTCCTGTAC ATACTTCAGC CACAGCAGCA AGGAAGATGT 
TGGCTTCATC GAAGGCAGTT AGATGGATCC CTAAACCGAA ACCCATCTGG CTTCTACGAC 
AGAGTCTGGC AGATCCTGGA GAGGACACCC AGTGGTATCA TAGTTGCTGG CAAATTTCTA 
CCCCAACAAC CAACTCTGTC TGACATGACA ATGTATGAGA TGAACTTCTC CCTTCTGGTA 
GAAGACATGT TACAGAACAT CGCCCAGCCT GAATATCGAC AAATTGTTGT TGAGCTGCTA 
ATGGTTGTTT CTGTTATCCT GGAGAGAAAC CCGGAGTTTG AATTTCAAGA TAAAGTTGAT 
TTAGACAAAC TAGTCAAAGA GGCATTTAAT GATTTCCAGC AAGACCAGAG CAGACTTCAG 
GGAGCGGAGA AGCAGGATGA CATGACGATG TTTTACAATA CTCCCCCACT GGGCAAGCGA 
GGGACCTGCA GCTACCTGAC AAAAGCAGTG ATGAATATGC TGCTGGAAGC TGAAGTCAAG 
CCAAGTACTG AGGACCCTTG CATTGTTAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_004914059.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914116.2 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa56951
Clone ID Related Accession (Same CDS sequence)	XM_012961648.3 , XM_004914059.4
Accession Version	XM_004914059.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914059.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GCTCCGTGTA TGAACCAATT AAGAGCTCGG ACCTACCCAG GCCAGACCAT 
GAAAGCCTGT GGGAAAAATT AGATCGGTAT TATAGGATTG TGAAAATCAC AGTGTTGAAT 
TATCAGAGTC CACAAACTGG TTTGTTCCCG ACCAAAACTG CTGGTGACTT TAAGAACGCG 
AAGATCCATG ACAGTCTATA CTGTGCTGCC AGCGCCTGGG CCCTCGCTCT TGCATACAGG 
CGCATTGATG ATGACAAGGG AAGAACCCAT GAGCTGGAGC ATTCTGCTAT AAAATGTATG 
AGAGGAATTC TGTACTGTTA CATGCGGCAG GCTGATAAGG TTGAGCAGTT TAAACAGGAT 
CCAAGTCCAC AAAAATGCCT TCATTCTATA TTCAATATAG ACAATGGAGA TGAGATATTA 
TCCTATAACG ATTATGGACA TATACAGATA AACGCAGTGT CACTTTTCCT TTTGTATCTT 
GTTGAGATGA TATCATCTGG ACTACAGATA ATCTACAATA CAGATGAGGT GTCGTTCATT 
CAGAACCTTG TATTCTGTGT AGAAAGAGCA TACCGTGTTC CTGACTTTGG AATCTGGGAA 
AGGGGCAGCA AGTATAACAA TGGCAGCACA GAACTACATT CCAGTTCTGT TGGTTTGGCT 
AAAGCTGCCC TTGAAGCCAT TGATGGATTC AACCTTTTTG GAAACCAGGG CTGCTCTTGG 
TCTGTCATCT TTGTGGATCT TGATGCTCAT AACAGGAACA GGCAAACTCT GAGTTCATTG 
CTTCCCAGAG AGTCCCGATC CCATAACACA GATGCAGCCC TCCTTACCTG TATCAGTTAC 
CCTGCATTTG CTGTTGATGA TGATGCTCTG TTTAACCAAA CACTGGATAA AATAGAACGT 
AAGCTTAAAG GAAAATATGG CTTTAAGCGA TTTCTAAGGG ATGGTTATCG GACTGCACTA 
GAAGATGAAA ACAGACGGTA CTACAAACCT GCTGAAATCA AGCTATTTGA TGGAATTGAA 
TGTGAATTCC CTTTGTTTTT TATCTACATG ATCATTGATG GTGTGTTCAG AGGGAAAGCT 
GAGCAAGTGA AAGAATATGA AGCACTCTTA GATCCTGTGC TATTTTATGG ACTTGATGGC 
TGTCCCGTGG TTCCAAAATA TTACTATGTG CCTGCTGATT TTGTTGAAGC AGAGAAGATG 
AACCCAAACA GCCAAAAGAG ATTTCCTAGC AACTGTGGGC GAGATGGAAA ACTCTTCCTC 
TGGGGACAAG CTCTGTACAT TATTGCTAAA CTGCTGGCCG AAGAATTAAT AAGTCCAAAA 
GACATTGATC CAATGAACCG TTTTGTTCAT CTTCGGGATC AGCGAAACAT CAGAATGCGG 
TACTCTAACC AGGGCCCCTT AGAAAATGAT GCCATGATAC ATGTGGCACT GATAGCTGAA 
AGCCAACGAT TGCAGGTTTT TCTCAACACT TATGGCATCC AAACCCAGAC CCCACAGCAA 
GTGGAACCTA TTCAGATATG GCCTCAGCAA GAACTTGTGA AGGCTTATCT TCACTTAGGA 
GTAAATAACA AGTTGGGGCT TTCTGGAAGG CCTGACAGGC CAATTGGATG CCTTGGCACA 
TCAAAGATTT ATCGTATTCT TGGGAAGACC GTTGTTTCCT ACCCTATTGT CTTTGATTTA 
AGTGACTTTT ACATGTCACA GGATGTGATG CTTCTGATCG ATGACATACA GAATGCTTTG 
CAGTTCATTA GGCAGTATTG GAAAATGAAT GGACGTCCCT TGTTTTTGGT TCTCATTCGG 
GAAGATAACA TCAGGGGAAG TCGATTTAAT CCAATCTTAG ACATGTTGGC TTCCTTTAAG 
AATGGAAATG TGGCCGGTGT GAAGGTTCAT GTGGACAGAC TGCAGACCTT AATATCCGGT 
GCAGTGGTTG AGCAGCTGGA TTTCTTGAGG ATCAATGAGA CAGAGGAGGT GCCTGAATTC 
AAAAGTTTTG AAGAGCTTGA AGTACCGAAA CATTCAAAGG TTAACAGGCA AGCAAGCATC 
CCTATTGCAG ATATGGAGCA TCAGCCAGAA ATAAACATGG GAGAATGGAA ACTCAAACCA 
ACCTATGAGG TTCTCGAGAA ACTAAATGAC TGCACCTGCC TGGCAAGCCA GGCCATCTTA 
CTAAGTATCT TACTGAAGAG AGAAGGACCT CATTTTATAA CCAAGGAAGG TACTGTCTCT 
GATTTTATGG CAAGAATCTA TAGAAAAGCT GGAAGCAATA AACTGTGGTC GGTTGTACGT 
CTTGCTGCCA GTCTTTTAGG TAAAATAGTG GACAGCCTTG CACCCTCTAT AACTAATGTT 
TTAGTTCAGG GCAAACAGGT AACACTGGGA GTCTTTGGCC ACGAGGAAGC GGTTATTTCC 
AATCCCTTGT CTCCAGGAGT GATCAAGGAT ATCCTCTACA AAAAGTGCAG CCCCTATGAT 
GAGCGAGAGG CTGTGCTGCA ACAAGAGCTA GTTATTCACA TTGGCTGGAT TATCTCCAAC 
ACACCAGAGC TTTTCAGCGG AATGTTAAAG ATACGGGTCG GATGGATTGT GCATGCAATG 
AGGTATGAAC TGAAGATCCG CAGTGGAGAT CAGCTAGAAA GGGACATCTA CCAGCTGTCT 
CCAGGAGAAA TCAAAGAGCT TCTCCTGTAC ATACTTCAGC CACAGCAGCA AGGAAGATGT 
TGGCTTCATC GAAGGCAGTT AGATGGATCC CTAAACCGAA ACCCATCTGG CTTCTACGAC 
AGAGTCTGGC AGATCCTGGA GAGGACACCC AGTGGTATCA TAGTTGCTGG CAAATTTCTA 
CCCCAACAAC CAACTCTGTC TGACATGACA ATGTATGAGA TGAACTTCTC CCTTCTGGTA 
GAAGACATGT TACAGAACAT CGCCCAGCCT GAATATCGAC AAATTGTTGT TGAGCTGCTA 
ATGGTTGTTT CTGTTATCCT GGAGAGAAAC CCGGAGTTTG AATTTCAAGA TAAAGTTGAT 
TTAGACAAAC TAGTCAAAGA GGCATTTAAT GATTTCCAGC AAGACCAGAG CAGACTTCAG 
GGAGCGGAGA AGCAGGATGA CATGACGATG TTTTACAATA CTCCCCCACT GGGCAAGCGA 
GGGACCTGCA GCTACCTGAC AAAAGCAGTG ATGAATATGC TGCTGGAAGC TGAAGTCAAG 
CCAAGTACTG AGGACCCTTG CATTGTTAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914116.2
CDS	183..3455
Translation	MQPIEMANSVDKAVSFSPTFLRSGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILSYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_004914059.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914059.4

ORF Insert Sequence:

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GCTCCGTGTA TGAACCAATT AAGAGCTCGG ACCTACCCAG GCCAGACCAT 
GAAAGCCTGT GGGAAAAATT AGATCGGTAT TATAGGATTG TGAAAATCAC AGTGTTGAAT 
TATCAGAGTC CACAAACTGG TTTGTTCCCG ACCAAAACTG CTGGTGACTT TAAGAACGCG 
AAGATCCATG ACAGTCTATA CTGTGCTGCC AGCGCCTGGG CCCTCGCTCT TGCATACAGG 
CGCATTGATG ATGACAAGGG AAGAACCCAT GAGCTGGAGC ATTCTGCTAT AAAATGTATG 
AGAGGAATTC TGTACTGTTA CATGCGGCAG GCTGATAAGG TTGAGCAGTT TAAACAGGAT 
CCAAGTCCAC AAAAATGCCT TCATTCTATA TTCAATATAG ACAATGGAGA TGAGATATTA 
TCCTATAACG ATTATGGACA TATACAGATA AACGCAGTGT CACTTTTCCT TTTGTATCTT 
GTTGAGATGA TATCATCTGG ACTACAGATA ATCTACAATA CAGATGAGGT GTCGTTCATT 
CAGAACCTTG TATTCTGTGT AGAAAGAGCA TACCGTGTTC CTGACTTTGG AATCTGGGAA 
AGGGGCAGCA AGTATAACAA TGGCAGCACA GAACTACATT CCAGTTCTGT TGGTTTGGCT 
AAAGCTGCCC TTGAAGCCAT TGATGGATTC AACCTTTTTG GAAACCAGGG CTGCTCTTGG 
TCTGTCATCT TTGTGGATCT TGATGCTCAT AACAGGAACA GGCAAACTCT GAGTTCATTG 
CTTCCCAGAG AGTCCCGATC CCATAACACA GATGCAGCCC TCCTTACCTG TATCAGTTAC 
CCTGCATTTG CTGTTGATGA TGATGCTCTG TTTAACCAAA CACTGGATAA AATAGAACGT 
AAGCTTAAAG GAAAATATGG CTTTAAGCGA TTTCTAAGGG ATGGTTATCG GACTGCACTA 
GAAGATGAAA ACAGACGGTA CTACAAACCT GCTGAAATCA AGCTATTTGA TGGAATTGAA 
TGTGAATTCC CTTTGTTTTT TATCTACATG ATCATTGATG GTGTGTTCAG AGGGAAAGCT 
GAGCAAGTGA AAGAATATGA AGCACTCTTA GATCCTGTGC TATTTTATGG ACTTGATGGC 
TGTCCCGTGG TTCCAAAATA TTACTATGTG CCTGCTGATT TTGTTGAAGC AGAGAAGATG 
AACCCAAACA GCCAAAAGAG ATTTCCTAGC AACTGTGGGC GAGATGGAAA ACTCTTCCTC 
TGGGGACAAG CTCTGTACAT TATTGCTAAA CTGCTGGCCG AAGAATTAAT AAGTCCAAAA 
GACATTGATC CAATGAACCG TTTTGTTCAT CTTCGGGATC AGCGAAACAT CAGAATGCGG 
TACTCTAACC AGGGCCCCTT AGAAAATGAT GCCATGATAC ATGTGGCACT GATAGCTGAA 
AGCCAACGAT TGCAGGTTTT TCTCAACACT TATGGCATCC AAACCCAGAC CCCACAGCAA 
GTGGAACCTA TTCAGATATG GCCTCAGCAA GAACTTGTGA AGGCTTATCT TCACTTAGGA 
GTAAATAACA AGTTGGGGCT TTCTGGAAGG CCTGACAGGC CAATTGGATG CCTTGGCACA 
TCAAAGATTT ATCGTATTCT TGGGAAGACC GTTGTTTCCT ACCCTATTGT CTTTGATTTA 
AGTGACTTTT ACATGTCACA GGATGTGATG CTTCTGATCG ATGACATACA GAATGCTTTG 
CAGTTCATTA GGCAGTATTG GAAAATGAAT GGACGTCCCT TGTTTTTGGT TCTCATTCGG 
GAAGATAACA TCAGGGGAAG TCGATTTAAT CCAATCTTAG ACATGTTGGC TTCCTTTAAG 
AATGGAAATG TGGCCGGTGT GAAGGTTCAT GTGGACAGAC TGCAGACCTT AATATCCGGT 
GCAGTGGTTG AGCAGCTGGA TTTCTTGAGG ATCAATGAGA CAGAGGAGGT GCCTGAATTC 
AAAAGTTTTG AAGAGCTTGA AGTACCGAAA CATTCAAAGG TTAACAGGCA AGCAAGCATC 
CCTATTGCAG ATATGGAGCA TCAGCCAGAA ATAAACATGG GAGAATGGAA ACTCAAACCA 
ACCTATGAGG TTCTCGAGAA ACTAAATGAC TGCACCTGCC TGGCAAGCCA GGCCATCTTA 
CTAAGTATCT TACTGAAGAG AGAAGGACCT CATTTTATAA CCAAGGAAGG TACTGTCTCT 
GATTTTATGG CAAGAATCTA TAGAAAAGCT GGAAGCAATA AACTGTGGTC GGTTGTACGT 
CTTGCTGCCA GTCTTTTAGG TAAAATAGTG GACAGCCTTG CACCCTCTAT AACTAATGTT 
TTAGTTCAGG GCAAACAGGT AACACTGGGA GTCTTTGGCC ACGAGGAAGC GGTTATTTCC 
AATCCCTTGT CTCCAGGAGT GATCAAGGAT ATCCTCTACA AAAAGTGCAG CCCCTATGAT 
GAGCGAGAGG CTGTGCTGCA ACAAGAGCTA GTTATTCACA TTGGCTGGAT TATCTCCAAC 
ACACCAGAGC TTTTCAGCGG AATGTTAAAG ATACGGGTCG GATGGATTGT GCATGCAATG 
AGGTATGAAC TGAAGATCCG CAGTGGAGAT CAGCTAGAAA GGGACATCTA CCAGCTGTCT 
CCAGGAGAAA TCAAAGAGCT TCTCCTGTAC ATACTTCAGC CACAGCAGCA AGGAAGATGT 
TGGCTTCATC GAAGGCAGTT AGATGGATCC CTAAACCGAA ACCCATCTGG CTTCTACGAC 
AGAGTCTGGC AGATCCTGGA GAGGACACCC AGTGGTATCA TAGTTGCTGG CAAATTTCTA 
CCCCAACAAC CAACTCTGTC TGACATGACA ATGTATGAGA TGAACTTCTC CCTTCTGGTA 
GAAGACATGT TACAGAACAT CGCCCAGCCT GAATATCGAC AAATTGTTGT TGAGCTGCTA 
ATGGTTGTTT CTGTTATCCT GGAGAGAAAC CCGGAGTTTG AATTTCAAGA TAAAGTTGAT 
TTAGACAAAC TAGTCAAAGA GGCATTTAAT GATTTCCAGC AAGACCAGAG CAGACTTCAG 
GGAGCGGAGA AGCAGGATGA CATGACGATG TTTTACAATA CTCCCCCACT GGGCAAGCGA 
GGGACCTGCA GCTACCTGAC AAAAGCAGTG ATGAATATGC TGCTGGAAGC TGAAGTCAAG 
CCAAGTACTG AGGACCCTTG CATTGTTAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_004914060.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914117.2 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X7, mRNA.

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CloneID	OXa56952
Clone ID Related Accession (Same CDS sequence)	XM_004914060.4
Accession Version	XM_004914060.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914060.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGG TCCTGGCCGA TGTTAGCTGG AAGGTGCTCG AGTTAAGGGC CAGATCTAAA 
CGCTCAGGCT CCGTGTATGA ACCAATTAAG AGCTCGGACC TACCCAGGCC AGACCATGAA 
AGCCTGTGGG AAAAATTAGA TCGGTATTAT AGGATTGTGA AAATCACAGT GTTGAATTAT 
CAGAGTCCAC AAACTGGTTT GTTCCCGACC AAAACTGCTG GTGACTTTAA GAACGCGAAG 
ATCCATGACA GTCTATACTG TGCTGCCAGC GCCTGGGCCC TCGCTCTTGC ATACAGGCGC 
ATTGATGATG ACAAGGGAAG AACCCATGAG CTGGAGCATT CTGCTATAAA ATGTATGAGA 
GGAATTCTGT ACTGTTACAT GCGGCAGGCT GATAAGGTTG AGCAGTTTAA ACAGGATCCA 
AGTCCACAAA AATGCCTTCA TTCTATATTC AATATAGACA ATGGAGATGA GATATTATCC 
TATAACGATT ATGGACATAT ACAGATAAAC GCAGTGTCAC TTTTCCTTTT GTATCTTGTT 
GAGATGATAT CATCTGGACT ACAGATAATC TACAATACAG ATGAGGTGTC GTTCATTCAG 
AACCTTGTAT TCTGTGTAGA AAGAGCATAC CGTGTTCCTG ACTTTGGAAT CTGGGAAAGG 
GGCAGCAAGT ATAACAATGG CAGCACAGAA CTACATTCCA GTTCTGTTGG TTTGGCTAAA 
GCTGCCCTTG AAGCCATTGA TGGATTCAAC CTTTTTGGAA ACCAGGGCTG CTCTTGGTCT 
GTCATCTTTG TGGATCTTGA TGCTCATAAC AGGAACAGGC AAACTCTGAG TTCATTGCTT 
CCCAGAGAGT CCCGATCCCA TAACACAGAT GCAGCCCTCC TTACCTGTAT CAGTTACCCT 
GCATTTGCTG TTGATGATGA TGCTCTGTTT AACCAAACAC TGGATAAAAT AGAACGTAAG 
CTTAAAGGAA AATATGGCTT TAAGCGATTT CTAAGGGATG GTTATCGGAC TGCACTAGAA 
GATGAAAACA GACGGTACTA CAAACCTGCT GAAATCAAGC TATTTGATGG AATTGAATGT 
GAATTCCCTT TGTTTTTTAT CTACATGATC ATTGATGGTG TGTTCAGAGG GAAAGCTGAG 
CAAGTGAAAG AATATGAAGC ACTCTTAGAT CCTGTGCTAT TTTATGGACT TGATGGCTGT 
CCCGTGGTTC CAAAATATTA CTATGTGCCT GCTGATTTTG TTGAAGCAGA GAAGATGAAC 
CCAAACAGCC AAAAGAGATT TCCTAGCAAC TGTGGGCGAG ATGGAAAACT CTTCCTCTGG 
GGACAAGCTC TGTACATTAT TGCTAAACTG CTGGCCGAAG AATTAATAAG TCCAAAAGAC 
ATTGATCCAA TGAACCGTTT TGTTCATCTT CGGGATCAGC GAAACATCAG AATGCGGTAC 
TCTAACCAGG GCCCCTTAGA AAATGATGCC ATGATACATG TGGCACTGAT AGCTGAAAGC 
CAACGATTGC AGGTTTTTCT CAACACTTAT GGCATCCAAA CCCAGACCCC ACAGCAAGTG 
GAACCTATTC AGATATGGCC TCAGCAAGAA CTTGTGAAGG CTTATCTTCA CTTAGGAGTA 
AATAACAAGT TGGGGCTTTC TGGAAGGCCT GACAGGCCAA TTGGATGCCT TGGCACATCA 
AAGATTTATC GTATTCTTGG GAAGACCGTT GTTTCCTACC CTATTGTCTT TGATTTAAGT 
GACTTTTACA TGTCACAGGA TGTGATGCTT CTGATCGATG ACATACAGAA TGCTTTGCAG 
TTCATTAGGC AGTATTGGAA AATGAATGGA CGTCCCTTGT TTTTGGTTCT CATTCGGGAA 
GATAACATCA GGGGAAGTCG ATTTAATCCA ATCTTAGACA TGTTGGCTTC CTTTAAGAAT 
GGAAATGTGG CCGGTGTGAA GGTTCATGTG GACAGACTGC AGACCTTAAT ATCCGGTGCA 
GTGGTTGAGC AGCTGGATTT CTTGAGGATC AATGAGACAG AGGAGGTGCC TGAATTCAAA 
AGTTTTGAAG AGCTTGAAGT ACCGAAACAT TCAAAGGTTA ACAGGCAAGC AAGCATCCCT 
ATTGCAGATA TGGAGCATCA GCCAGAAATA AACATGGGAG AATGGAAACT CAAACCAACC 
TATGAGGTTC TCGAGAAACT AAATGACTGC ACCTGCCTGG CAAGCCAGGC CATCTTACTA 
AGTATCTTAC TGAAGAGAGA AGGACCTCAT TTTATAACCA AGGAAGGTAC TGTCTCTGAT 
TTTATGGCAA GAATCTATAG AAAAGCTGGA AGCAATAAAC TGTGGTCGGT TGTACGTCTT 
GCTGCCAGTC TTTTAGGTAA AATAGTGGAC AGCCTTGCAC CCTCTATAAC TAATGTTTTA 
GTTCAGGGCA AACAGGTAAC ACTGGGAGTC TTTGGCCACG AGGAAGCGGT TATTTCCAAT 
CCCTTGTCTC CAGGAGTGAT CAAGGATATC CTCTACAAAA AGTGCAGCCC CTATGATGAG 
CGAGAGGCTG TGCTGCAACA AGAGCTAGTT ATTCACATTG GCTGGATTAT CTCCAACACA 
CCAGAGCTTT TCAGCGGAAT GTTAAAGATA CGGGTCGGAT GGATTGTGCA TGCAATGAGG 
TATGAACTGA AGATCCGCAG TGGAGATCAG CTAGAAAGGG ACATCTACCA GCTGTCTCCA 
GGAGAAATCA AAGAGCTTCT CCTGTACATA CTTCAGCCAC AGCAGCAAGG AAGATGTTGG 
CTTCATCGAA GGCAGTTAGA TGGATCCCTA AACCGAAACC CATCTGGCTT CTACGACAGA 
GTCTGGCAGA TCCTGGAGAG GACACCCAGT GGTATCATAG TTGCTGGCAA ATTTCTACCC 
CAACAACCAA CTCTGTCTGA CATGACAATG TATGAGATGA ACTTCTCCCT TCTGGTAGAA 
GACATGTTAC AGAACATCGC CCAGCCTGAA TATCGACAAA TTGTTGTTGA GCTGCTAATG 
GTTGTTTCTG TTATCCTGGA GAGAAACCCG GAGTTTGAAT TTCAAGATAA AGTTGATTTA 
GACAAACTAG TCAAAGAGGC ATTTAATGAT TTCCAGCAAG ACCAGAGCAG ACTTCAGGGA 
GCGGAGAAGC AGGATGACAT GACGATGTTT TACAATACTC CCCCACTGGG CAAGCGAGGG 
ACCTGCAGCT ACCTGACAAA AGCAGTGATG AATATGCTGC TGGAAGCTGA AGTCAAGCCA 
AGTACTGAGG ACCCTTGCAT TGTTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914117.2
CDS	63..3332
Translation	MAQVLADVSWKVLELRARSKRSGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILSYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_004914060.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914060.4

ORF Insert Sequence:

ATGGCTCAGG TCCTGGCCGA TGTTAGCTGG AAGGTGCTCG AGTTAAGGGC CAGATCTAAA 
CGCTCAGGCT CCGTGTATGA ACCAATTAAG AGCTCGGACC TACCCAGGCC AGACCATGAA 
AGCCTGTGGG AAAAATTAGA TCGGTATTAT AGGATTGTGA AAATCACAGT GTTGAATTAT 
CAGAGTCCAC AAACTGGTTT GTTCCCGACC AAAACTGCTG GTGACTTTAA GAACGCGAAG 
ATCCATGACA GTCTATACTG TGCTGCCAGC GCCTGGGCCC TCGCTCTTGC ATACAGGCGC 
ATTGATGATG ACAAGGGAAG AACCCATGAG CTGGAGCATT CTGCTATAAA ATGTATGAGA 
GGAATTCTGT ACTGTTACAT GCGGCAGGCT GATAAGGTTG AGCAGTTTAA ACAGGATCCA 
AGTCCACAAA AATGCCTTCA TTCTATATTC AATATAGACA ATGGAGATGA GATATTATCC 
TATAACGATT ATGGACATAT ACAGATAAAC GCAGTGTCAC TTTTCCTTTT GTATCTTGTT 
GAGATGATAT CATCTGGACT ACAGATAATC TACAATACAG ATGAGGTGTC GTTCATTCAG 
AACCTTGTAT TCTGTGTAGA AAGAGCATAC CGTGTTCCTG ACTTTGGAAT CTGGGAAAGG 
GGCAGCAAGT ATAACAATGG CAGCACAGAA CTACATTCCA GTTCTGTTGG TTTGGCTAAA 
GCTGCCCTTG AAGCCATTGA TGGATTCAAC CTTTTTGGAA ACCAGGGCTG CTCTTGGTCT 
GTCATCTTTG TGGATCTTGA TGCTCATAAC AGGAACAGGC AAACTCTGAG TTCATTGCTT 
CCCAGAGAGT CCCGATCCCA TAACACAGAT GCAGCCCTCC TTACCTGTAT CAGTTACCCT 
GCATTTGCTG TTGATGATGA TGCTCTGTTT AACCAAACAC TGGATAAAAT AGAACGTAAG 
CTTAAAGGAA AATATGGCTT TAAGCGATTT CTAAGGGATG GTTATCGGAC TGCACTAGAA 
GATGAAAACA GACGGTACTA CAAACCTGCT GAAATCAAGC TATTTGATGG AATTGAATGT 
GAATTCCCTT TGTTTTTTAT CTACATGATC ATTGATGGTG TGTTCAGAGG GAAAGCTGAG 
CAAGTGAAAG AATATGAAGC ACTCTTAGAT CCTGTGCTAT TTTATGGACT TGATGGCTGT 
CCCGTGGTTC CAAAATATTA CTATGTGCCT GCTGATTTTG TTGAAGCAGA GAAGATGAAC 
CCAAACAGCC AAAAGAGATT TCCTAGCAAC TGTGGGCGAG ATGGAAAACT CTTCCTCTGG 
GGACAAGCTC TGTACATTAT TGCTAAACTG CTGGCCGAAG AATTAATAAG TCCAAAAGAC 
ATTGATCCAA TGAACCGTTT TGTTCATCTT CGGGATCAGC GAAACATCAG AATGCGGTAC 
TCTAACCAGG GCCCCTTAGA AAATGATGCC ATGATACATG TGGCACTGAT AGCTGAAAGC 
CAACGATTGC AGGTTTTTCT CAACACTTAT GGCATCCAAA CCCAGACCCC ACAGCAAGTG 
GAACCTATTC AGATATGGCC TCAGCAAGAA CTTGTGAAGG CTTATCTTCA CTTAGGAGTA 
AATAACAAGT TGGGGCTTTC TGGAAGGCCT GACAGGCCAA TTGGATGCCT TGGCACATCA 
AAGATTTATC GTATTCTTGG GAAGACCGTT GTTTCCTACC CTATTGTCTT TGATTTAAGT 
GACTTTTACA TGTCACAGGA TGTGATGCTT CTGATCGATG ACATACAGAA TGCTTTGCAG 
TTCATTAGGC AGTATTGGAA AATGAATGGA CGTCCCTTGT TTTTGGTTCT CATTCGGGAA 
GATAACATCA GGGGAAGTCG ATTTAATCCA ATCTTAGACA TGTTGGCTTC CTTTAAGAAT 
GGAAATGTGG CCGGTGTGAA GGTTCATGTG GACAGACTGC AGACCTTAAT ATCCGGTGCA 
GTGGTTGAGC AGCTGGATTT CTTGAGGATC AATGAGACAG AGGAGGTGCC TGAATTCAAA 
AGTTTTGAAG AGCTTGAAGT ACCGAAACAT TCAAAGGTTA ACAGGCAAGC AAGCATCCCT 
ATTGCAGATA TGGAGCATCA GCCAGAAATA AACATGGGAG AATGGAAACT CAAACCAACC 
TATGAGGTTC TCGAGAAACT AAATGACTGC ACCTGCCTGG CAAGCCAGGC CATCTTACTA 
AGTATCTTAC TGAAGAGAGA AGGACCTCAT TTTATAACCA AGGAAGGTAC TGTCTCTGAT 
TTTATGGCAA GAATCTATAG AAAAGCTGGA AGCAATAAAC TGTGGTCGGT TGTACGTCTT 
GCTGCCAGTC TTTTAGGTAA AATAGTGGAC AGCCTTGCAC CCTCTATAAC TAATGTTTTA 
GTTCAGGGCA AACAGGTAAC ACTGGGAGTC TTTGGCCACG AGGAAGCGGT TATTTCCAAT 
CCCTTGTCTC CAGGAGTGAT CAAGGATATC CTCTACAAAA AGTGCAGCCC CTATGATGAG 
CGAGAGGCTG TGCTGCAACA AGAGCTAGTT ATTCACATTG GCTGGATTAT CTCCAACACA 
CCAGAGCTTT TCAGCGGAAT GTTAAAGATA CGGGTCGGAT GGATTGTGCA TGCAATGAGG 
TATGAACTGA AGATCCGCAG TGGAGATCAG CTAGAAAGGG ACATCTACCA GCTGTCTCCA 
GGAGAAATCA AAGAGCTTCT CCTGTACATA CTTCAGCCAC AGCAGCAAGG AAGATGTTGG 
CTTCATCGAA GGCAGTTAGA TGGATCCCTA AACCGAAACC CATCTGGCTT CTACGACAGA 
GTCTGGCAGA TCCTGGAGAG GACACCCAGT GGTATCATAG TTGCTGGCAA ATTTCTACCC 
CAACAACCAA CTCTGTCTGA CATGACAATG TATGAGATGA ACTTCTCCCT TCTGGTAGAA 
GACATGTTAC AGAACATCGC CCAGCCTGAA TATCGACAAA TTGTTGTTGA GCTGCTAATG 
GTTGTTTCTG TTATCCTGGA GAGAAACCCG GAGTTTGAAT TTCAAGATAA AGTTGATTTA 
GACAAACTAG TCAAAGAGGC ATTTAATGAT TTCCAGCAAG ACCAGAGCAG ACTTCAGGGA 
GCGGAGAAGC AGGATGACAT GACGATGTTT TACAATACTC CCCCACTGGG CAAGCGAGGG 
ACCTGCAGCT ACCTGACAAA AGCAGTGATG AATATGCTGC TGGAAGCTGA AGTCAAGCCA 
AGTACTGAGG ACCCTTGCAT TGTTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_012961646.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817100.1 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X3, mRNA.

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CloneID	OXa09606
Clone ID Related Accession (Same CDS sequence)	XM_012961646.2 , XM_002931669.3 , XM_012961645.2
Accession Version	XM_012961646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTACCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAATT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGACGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGAGAGAT 
GGAAAACTCT TTCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTCGGTTG TACGTCTTGC TGCCAGTCTT TTAGGTAAAA TAGTGGACAG CCTTGCACCC 
TCTATAACTA ATGTTTTAGT TCAGGGCAAA CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817100.1
CDS	108..3395
Translation	MQPIEMANSVDKAVSFSPTFLRSGKESYGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILPYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_012961646.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961646.2

ORF Insert Sequence:

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTACCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAATT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGACGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGAGAGAT 
GGAAAACTCT TTCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTCGGTTG TACGTCTTGC TGCCAGTCTT TTAGGTAAAA TAGTGGACAG CCTTGCACCC 
TCTATAACTA ATGTTTTAGT TCAGGGCAAA CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_002931669.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931715.2 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X1, mRNA.

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CloneID	OXa09606
Clone ID Related Accession (Same CDS sequence)	XM_012961646.2 , XM_002931669.3 , XM_012961645.2
Accession Version	XM_002931669.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002931669.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTACCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAATT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGACGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGAGAGAT 
GGAAAACTCT TTCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTCGGTTG TACGTCTTGC TGCCAGTCTT TTAGGTAAAA TAGTGGACAG CCTTGCACCC 
TCTATAACTA ATGTTTTAGT TCAGGGCAAA CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931715.2
CDS	182..3469
Translation	MQPIEMANSVDKAVSFSPTFLRSGKESYGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILPYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_002931669.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931669.3

ORF Insert Sequence:

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTACCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAATT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGACGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGAGAGAT 
GGAAAACTCT TTCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTCGGTTG TACGTCTTGC TGCCAGTCTT TTAGGTAAAA TAGTGGACAG CCTTGCACCC 
TCTATAACTA ATGTTTTAGT TCAGGGCAAA CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_012961645.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817099.1 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X2, mRNA.

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CloneID	OXa09606
Clone ID Related Accession (Same CDS sequence)	XM_012961646.2 , XM_002931669.3 , XM_012961645.2
Accession Version	XM_012961645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTACCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAATT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGACGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGAGAGAT 
GGAAAACTCT TTCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTCGGTTG TACGTCTTGC TGCCAGTCTT TTAGGTAAAA TAGTGGACAG CCTTGCACCC 
TCTATAACTA ATGTTTTAGT TCAGGGCAAA CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817099.1
CDS	112..3399
Translation	MQPIEMANSVDKAVSFSPTFLRSGKESYGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILPYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_012961645.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961645.2

ORF Insert Sequence:

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GGAAGGAATC CTATGGCTCC GTGTATGAAC CAATTAAGAG CTCGGACCTA 
CCCAGGCCAG ACCATGAAAG CCTGTGGGAA AAATTAGATC GGTATTATAG GATTGTGAAA 
ATCACAGTGT TGAATTATCA GAGTCCACAA ACTGGTTTGT TCCCGACCAA AACTGCTGGT 
GACTTTAAGA ACGCGAAGAT CCATGACAGT CTATACTGTG CTGCCAGCGC CTGGGCCCTC 
GCTCTTGCAT ACAGGCGCAT TGATGATGAC AAGGGAAGAA CCCATGAGCT GGAGCATTCT 
GCTATAAAAT GTATGAGAGG AATTCTGTAC TGTTACATGC GGCAGGCTGA TAAGGTTGAG 
CAGTTTAAAC AGGATCCAAG TCCACAAAAA TGCCTTCATT CTATATTCAA TATAGACAAT 
GGAGATGAGA TATTACCCTA TAACGATTAT GGACATATAC AGATAAACGC AGTGTCACTT 
TTCCTTTTGT ATCTTGTTGA GATGATATCA TCTGGACTAC AGATAATCTA CAATACAGAT 
GAGGTGTCGT TCATTCAGAA CCTTGTATTC TGTGTAGAAA GAGCATACCG TGTTCCTGAC 
TTTGGAATCT GGGAAAGGGG CAGCAAGTAT AACAATGGCA GCACAGAATT ACATTCCAGT 
TCTGTTGGTT TGGCTAAAGC TGCCCTTGAA GCCATTGATG GATTCAACCT TTTTGGAAAC 
CAGGGCTGCT CTTGGTCTGT CATCTTTGTG GATCTTGATG CTCATAACAG GAACAGGCAA 
ACTCTGAGTT CATTGCTTCC CAGAGAGTCC CGATCCCATA ACACAGATGC AGCCCTCCTT 
ACCTGTATCA GTTACCCTGC ATTTGCTGTT GATGATGATG CTCTGTTTAA CCAAACACTG 
GATAAAATAG AACGTAAGCT TAAAGGAAAA TATGGCTTTA AGCGATTTCT AAGGGATGGT 
TATCGGACTG CACTAGAAGA TGAAAACAGA CGGTACTACA AACCTGCTGA AATCAAGCTA 
TTTGACGGAA TTGAATGTGA ATTCCCTTTG TTTTTTATCT ACATGATCAT TGATGGTGTG 
TTCAGAGGGA AAGCTGAGCA AGTGAAAGAA TATGAAGCAC TCTTAGATCC TGTGCTATTT 
TATGGACTTG ATGGCTGTCC CGTGGTTCCA AAATATTACT ATGTGCCTGC TGATTTTGTT 
GAAGCAGAGA AGATGAACCC AAACAGCCAA AAGAGATTTC CTAGCAACTG TGGGAGAGAT 
GGAAAACTCT TTCTCTGGGG ACAAGCTCTG TACATTATTG CTAAACTGCT GGCCGAAGAA 
TTAATAAGTC CAAAAGACAT TGATCCAATG AACCGTTTTG TTCATCTTCG GGATCAGCGA 
AACATCAGAA TGCGGTACTC TAACCAGGGC CCCTTAGAAA ATGATGCCAT GATACATGTG 
GCACTGATAG CTGAAAGCCA ACGATTGCAG GTTTTTCTCA ACACTTATGG CATCCAAACC 
CAGACCCCAC AGCAAGTGGA ACCTATTCAG ATATGGCCTC AGCAAGAACT TGTGAAGGCT 
TATCTTCACT TAGGAGTAAA TAACAAGTTG GGGCTTTCTG GAAGGCCTGA CAGGCCAATT 
GGATGCCTTG GCACATCAAA GATTTATCGT ATTCTTGGGA AGACCGTTGT TTCCTACCCT 
ATTGTCTTTG ATTTAAGTGA CTTTTACATG TCACAGGATG TGATGCTTCT GATCGATGAC 
ATACAGAATG CTTTGCAGTT CATTAGGCAG TATTGGAAAA TGAATGGACG TCCCTTGTTT 
TTGGTTCTCA TTCGGGAAGA TAACATCAGG GGAAGTCGAT TTAATCCAAT CTTAGACATG 
TTGGCTTCCT TTAAGAATGG AAATGTGGCC GGTGTGAAGG TTCATGTGGA CAGACTGCAG 
ACCTTAATAT CCGGTGCAGT GGTTGAGCAG CTGGATTTCT TGAGGATCAA TGAGACAGAG 
GAGGTGCCTG AATTCAAAAG TTTTGAAGAG CTTGAAGTAC CGAAACATTC AAAGGTTAAC 
AGGCAAGCAA GCATCCCTAT TGCAGATATG GAGCATCAGC CAGAAATAAA CATGGGAGAA 
TGGAAACTCA AACCAACCTA TGAGGTTCTC GAGAAACTAA ATGACTGCAC CTGCCTGGCA 
AGCCAGGCCA TCTTACTAAG TATCTTACTG AAGAGAGAAG GACCTCATTT TATAACCAAG 
GAAGGTACTG TCTCTGATTT TATGGCAAGA ATCTATAGAA AAGCTGGAAG CAATAAACTG 
TGGTCGGTTG TACGTCTTGC TGCCAGTCTT TTAGGTAAAA TAGTGGACAG CCTTGCACCC 
TCTATAACTA ATGTTTTAGT TCAGGGCAAA CAGGTAACAC TGGGAGTCTT TGGCCACGAG 
GAAGCGGTTA TTTCCAATCC CTTGTCTCCA GGAGTGATCA AGGATATCCT CTACAAAAAG 
TGCAGCCCCT ATGATGAGCG AGAGGCTGTG CTGCAACAAG AGCTAGTTAT TCACATTGGC 
TGGATTATCT CCAACACACC AGAGCTTTTC AGCGGAATGT TAAAGATACG GGTCGGATGG 
ATTGTGCATG CAATGAGGTA TGAACTGAAG ATCCGCAGTG GAGATCAGCT AGAAAGGGAC 
ATCTACCAGC TGTCTCCAGG AGAAATCAAA GAGCTTCTCC TGTACATACT TCAGCCACAG 
CAGCAAGGAA GATGTTGGCT TCATCGAAGG CAGTTAGATG GATCCCTAAA CCGAAACCCA 
TCTGGCTTCT ACGACAGAGT CTGGCAGATC CTGGAGAGGA CACCCAGTGG TATCATAGTT 
GCTGGCAAAT TTCTACCCCA ACAACCAACT CTGTCTGACA TGACAATGTA TGAGATGAAC 
TTCTCCCTTC TGGTAGAAGA CATGTTACAG AACATCGCCC AGCCTGAATA TCGACAAATT 
GTTGTTGAGC TGCTAATGGT TGTTTCTGTT ATCCTGGAGA GAAACCCGGA GTTTGAATTT 
CAAGATAAAG TTGATTTAGA CAAACTAGTC AAAGAGGCAT TTAATGATTT CCAGCAAGAC 
CAGAGCAGAC TTCAGGGAGC GGAGAAGCAG GATGACATGA CGATGTTTTA CAATACTCCC 
CCACTGGGCA AGCGAGGGAC CTGCAGCTAC CTGACAAAAG CAGTGATGAA TATGCTGCTG 
GAAGCTGAAG TCAAGCCAAG TACTGAGGAC CCTTGCATTG TTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_004914059.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914116.1 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa09607
Clone ID Related Accession (Same CDS sequence)	XM_004914059.3 , XM_012961648.2
Accession Version	XM_004914059.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914059.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GCTCCGTGTA TGAACCAATT AAGAGCTCGG ACCTACCCAG GCCAGACCAT 
GAAAGCCTGT GGGAAAAATT AGATCGGTAT TATAGGATTG TGAAAATCAC AGTGTTGAAT 
TATCAGAGTC CACAAACTGG TTTGTTCCCG ACCAAAACTG CTGGTGACTT TAAGAACGCG 
AAGATCCATG ACAGTCTATA CTGTGCTGCC AGCGCCTGGG CCCTCGCTCT TGCATACAGG 
CGCATTGATG ATGACAAGGG AAGAACCCAT GAGCTGGAGC ATTCTGCTAT AAAATGTATG 
AGAGGAATTC TGTACTGTTA CATGCGGCAG GCTGATAAGG TTGAGCAGTT TAAACAGGAT 
CCAAGTCCAC AAAAATGCCT TCATTCTATA TTCAATATAG ACAATGGAGA TGAGATATTA 
CCCTATAACG ATTATGGACA TATACAGATA AACGCAGTGT CACTTTTCCT TTTGTATCTT 
GTTGAGATGA TATCATCTGG ACTACAGATA ATCTACAATA CAGATGAGGT GTCGTTCATT 
CAGAACCTTG TATTCTGTGT AGAAAGAGCA TACCGTGTTC CTGACTTTGG AATCTGGGAA 
AGGGGCAGCA AGTATAACAA TGGCAGCACA GAATTACATT CCAGTTCTGT TGGTTTGGCT 
AAAGCTGCCC TTGAAGCCAT TGATGGATTC AACCTTTTTG GAAACCAGGG CTGCTCTTGG 
TCTGTCATCT TTGTGGATCT TGATGCTCAT AACAGGAACA GGCAAACTCT GAGTTCATTG 
CTTCCCAGAG AGTCCCGATC CCATAACACA GATGCAGCCC TCCTTACCTG TATCAGTTAC 
CCTGCATTTG CTGTTGATGA TGATGCTCTG TTTAACCAAA CACTGGATAA AATAGAACGT 
AAGCTTAAAG GAAAATATGG CTTTAAGCGA TTTCTAAGGG ATGGTTATCG GACTGCACTA 
GAAGATGAAA ACAGACGGTA CTACAAACCT GCTGAAATCA AGCTATTTGA CGGAATTGAA 
TGTGAATTCC CTTTGTTTTT TATCTACATG ATCATTGATG GTGTGTTCAG AGGGAAAGCT 
GAGCAAGTGA AAGAATATGA AGCACTCTTA GATCCTGTGC TATTTTATGG ACTTGATGGC 
TGTCCCGTGG TTCCAAAATA TTACTATGTG CCTGCTGATT TTGTTGAAGC AGAGAAGATG 
AACCCAAACA GCCAAAAGAG ATTTCCTAGC AACTGTGGGA GAGATGGAAA ACTCTTTCTC 
TGGGGACAAG CTCTGTACAT TATTGCTAAA CTGCTGGCCG AAGAATTAAT AAGTCCAAAA 
GACATTGATC CAATGAACCG TTTTGTTCAT CTTCGGGATC AGCGAAACAT CAGAATGCGG 
TACTCTAACC AGGGCCCCTT AGAAAATGAT GCCATGATAC ATGTGGCACT GATAGCTGAA 
AGCCAACGAT TGCAGGTTTT TCTCAACACT TATGGCATCC AAACCCAGAC CCCACAGCAA 
GTGGAACCTA TTCAGATATG GCCTCAGCAA GAACTTGTGA AGGCTTATCT TCACTTAGGA 
GTAAATAACA AGTTGGGGCT TTCTGGAAGG CCTGACAGGC CAATTGGATG CCTTGGCACA 
TCAAAGATTT ATCGTATTCT TGGGAAGACC GTTGTTTCCT ACCCTATTGT CTTTGATTTA 
AGTGACTTTT ACATGTCACA GGATGTGATG CTTCTGATCG ATGACATACA GAATGCTTTG 
CAGTTCATTA GGCAGTATTG GAAAATGAAT GGACGTCCCT TGTTTTTGGT TCTCATTCGG 
GAAGATAACA TCAGGGGAAG TCGATTTAAT CCAATCTTAG ACATGTTGGC TTCCTTTAAG 
AATGGAAATG TGGCCGGTGT GAAGGTTCAT GTGGACAGAC TGCAGACCTT AATATCCGGT 
GCAGTGGTTG AGCAGCTGGA TTTCTTGAGG ATCAATGAGA CAGAGGAGGT GCCTGAATTC 
AAAAGTTTTG AAGAGCTTGA AGTACCGAAA CATTCAAAGG TTAACAGGCA AGCAAGCATC 
CCTATTGCAG ATATGGAGCA TCAGCCAGAA ATAAACATGG GAGAATGGAA ACTCAAACCA 
ACCTATGAGG TTCTCGAGAA ACTAAATGAC TGCACCTGCC TGGCAAGCCA GGCCATCTTA 
CTAAGTATCT TACTGAAGAG AGAAGGACCT CATTTTATAA CCAAGGAAGG TACTGTCTCT 
GATTTTATGG CAAGAATCTA TAGAAAAGCT GGAAGCAATA AACTGTGGTC GGTTGTACGT 
CTTGCTGCCA GTCTTTTAGG TAAAATAGTG GACAGCCTTG CACCCTCTAT AACTAATGTT 
TTAGTTCAGG GCAAACAGGT AACACTGGGA GTCTTTGGCC ACGAGGAAGC GGTTATTTCC 
AATCCCTTGT CTCCAGGAGT GATCAAGGAT ATCCTCTACA AAAAGTGCAG CCCCTATGAT 
GAGCGAGAGG CTGTGCTGCA ACAAGAGCTA GTTATTCACA TTGGCTGGAT TATCTCCAAC 
ACACCAGAGC TTTTCAGCGG AATGTTAAAG ATACGGGTCG GATGGATTGT GCATGCAATG 
AGGTATGAAC TGAAGATCCG CAGTGGAGAT CAGCTAGAAA GGGACATCTA CCAGCTGTCT 
CCAGGAGAAA TCAAAGAGCT TCTCCTGTAC ATACTTCAGC CACAGCAGCA AGGAAGATGT 
TGGCTTCATC GAAGGCAGTT AGATGGATCC CTAAACCGAA ACCCATCTGG CTTCTACGAC 
AGAGTCTGGC AGATCCTGGA GAGGACACCC AGTGGTATCA TAGTTGCTGG CAAATTTCTA 
CCCCAACAAC CAACTCTGTC TGACATGACA ATGTATGAGA TGAACTTCTC CCTTCTGGTA 
GAAGACATGT TACAGAACAT CGCCCAGCCT GAATATCGAC AAATTGTTGT TGAGCTGCTA 
ATGGTTGTTT CTGTTATCCT GGAGAGAAAC CCGGAGTTTG AATTTCAAGA TAAAGTTGAT 
TTAGACAAAC TAGTCAAAGA GGCATTTAAT GATTTCCAGC AAGACCAGAG CAGACTTCAG 
GGAGCGGAGA AGCAGGATGA CATGACGATG TTTTACAATA CTCCCCCACT GGGCAAGCGA 
GGGACCTGCA GCTACCTGAC AAAAGCAGTG ATGAATATGC TGCTGGAAGC TGAAGTCAAG 
CCAAGTACTG AGGACCCTTG CATTGTTAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914116.1
CDS	182..3454
Translation	MQPIEMANSVDKAVSFSPTFLRSGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILPYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_004914059.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914059.3

ORF Insert Sequence:

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GCTCCGTGTA TGAACCAATT AAGAGCTCGG ACCTACCCAG GCCAGACCAT 
GAAAGCCTGT GGGAAAAATT AGATCGGTAT TATAGGATTG TGAAAATCAC AGTGTTGAAT 
TATCAGAGTC CACAAACTGG TTTGTTCCCG ACCAAAACTG CTGGTGACTT TAAGAACGCG 
AAGATCCATG ACAGTCTATA CTGTGCTGCC AGCGCCTGGG CCCTCGCTCT TGCATACAGG 
CGCATTGATG ATGACAAGGG AAGAACCCAT GAGCTGGAGC ATTCTGCTAT AAAATGTATG 
AGAGGAATTC TGTACTGTTA CATGCGGCAG GCTGATAAGG TTGAGCAGTT TAAACAGGAT 
CCAAGTCCAC AAAAATGCCT TCATTCTATA TTCAATATAG ACAATGGAGA TGAGATATTA 
CCCTATAACG ATTATGGACA TATACAGATA AACGCAGTGT CACTTTTCCT TTTGTATCTT 
GTTGAGATGA TATCATCTGG ACTACAGATA ATCTACAATA CAGATGAGGT GTCGTTCATT 
CAGAACCTTG TATTCTGTGT AGAAAGAGCA TACCGTGTTC CTGACTTTGG AATCTGGGAA 
AGGGGCAGCA AGTATAACAA TGGCAGCACA GAATTACATT CCAGTTCTGT TGGTTTGGCT 
AAAGCTGCCC TTGAAGCCAT TGATGGATTC AACCTTTTTG GAAACCAGGG CTGCTCTTGG 
TCTGTCATCT TTGTGGATCT TGATGCTCAT AACAGGAACA GGCAAACTCT GAGTTCATTG 
CTTCCCAGAG AGTCCCGATC CCATAACACA GATGCAGCCC TCCTTACCTG TATCAGTTAC 
CCTGCATTTG CTGTTGATGA TGATGCTCTG TTTAACCAAA CACTGGATAA AATAGAACGT 
AAGCTTAAAG GAAAATATGG CTTTAAGCGA TTTCTAAGGG ATGGTTATCG GACTGCACTA 
GAAGATGAAA ACAGACGGTA CTACAAACCT GCTGAAATCA AGCTATTTGA CGGAATTGAA 
TGTGAATTCC CTTTGTTTTT TATCTACATG ATCATTGATG GTGTGTTCAG AGGGAAAGCT 
GAGCAAGTGA AAGAATATGA AGCACTCTTA GATCCTGTGC TATTTTATGG ACTTGATGGC 
TGTCCCGTGG TTCCAAAATA TTACTATGTG CCTGCTGATT TTGTTGAAGC AGAGAAGATG 
AACCCAAACA GCCAAAAGAG ATTTCCTAGC AACTGTGGGA GAGATGGAAA ACTCTTTCTC 
TGGGGACAAG CTCTGTACAT TATTGCTAAA CTGCTGGCCG AAGAATTAAT AAGTCCAAAA 
GACATTGATC CAATGAACCG TTTTGTTCAT CTTCGGGATC AGCGAAACAT CAGAATGCGG 
TACTCTAACC AGGGCCCCTT AGAAAATGAT GCCATGATAC ATGTGGCACT GATAGCTGAA 
AGCCAACGAT TGCAGGTTTT TCTCAACACT TATGGCATCC AAACCCAGAC CCCACAGCAA 
GTGGAACCTA TTCAGATATG GCCTCAGCAA GAACTTGTGA AGGCTTATCT TCACTTAGGA 
GTAAATAACA AGTTGGGGCT TTCTGGAAGG CCTGACAGGC CAATTGGATG CCTTGGCACA 
TCAAAGATTT ATCGTATTCT TGGGAAGACC GTTGTTTCCT ACCCTATTGT CTTTGATTTA 
AGTGACTTTT ACATGTCACA GGATGTGATG CTTCTGATCG ATGACATACA GAATGCTTTG 
CAGTTCATTA GGCAGTATTG GAAAATGAAT GGACGTCCCT TGTTTTTGGT TCTCATTCGG 
GAAGATAACA TCAGGGGAAG TCGATTTAAT CCAATCTTAG ACATGTTGGC TTCCTTTAAG 
AATGGAAATG TGGCCGGTGT GAAGGTTCAT GTGGACAGAC TGCAGACCTT AATATCCGGT 
GCAGTGGTTG AGCAGCTGGA TTTCTTGAGG ATCAATGAGA CAGAGGAGGT GCCTGAATTC 
AAAAGTTTTG AAGAGCTTGA AGTACCGAAA CATTCAAAGG TTAACAGGCA AGCAAGCATC 
CCTATTGCAG ATATGGAGCA TCAGCCAGAA ATAAACATGG GAGAATGGAA ACTCAAACCA 
ACCTATGAGG TTCTCGAGAA ACTAAATGAC TGCACCTGCC TGGCAAGCCA GGCCATCTTA 
CTAAGTATCT TACTGAAGAG AGAAGGACCT CATTTTATAA CCAAGGAAGG TACTGTCTCT 
GATTTTATGG CAAGAATCTA TAGAAAAGCT GGAAGCAATA AACTGTGGTC GGTTGTACGT 
CTTGCTGCCA GTCTTTTAGG TAAAATAGTG GACAGCCTTG CACCCTCTAT AACTAATGTT 
TTAGTTCAGG GCAAACAGGT AACACTGGGA GTCTTTGGCC ACGAGGAAGC GGTTATTTCC 
AATCCCTTGT CTCCAGGAGT GATCAAGGAT ATCCTCTACA AAAAGTGCAG CCCCTATGAT 
GAGCGAGAGG CTGTGCTGCA ACAAGAGCTA GTTATTCACA TTGGCTGGAT TATCTCCAAC 
ACACCAGAGC TTTTCAGCGG AATGTTAAAG ATACGGGTCG GATGGATTGT GCATGCAATG 
AGGTATGAAC TGAAGATCCG CAGTGGAGAT CAGCTAGAAA GGGACATCTA CCAGCTGTCT 
CCAGGAGAAA TCAAAGAGCT TCTCCTGTAC ATACTTCAGC CACAGCAGCA AGGAAGATGT 
TGGCTTCATC GAAGGCAGTT AGATGGATCC CTAAACCGAA ACCCATCTGG CTTCTACGAC 
AGAGTCTGGC AGATCCTGGA GAGGACACCC AGTGGTATCA TAGTTGCTGG CAAATTTCTA 
CCCCAACAAC CAACTCTGTC TGACATGACA ATGTATGAGA TGAACTTCTC CCTTCTGGTA 
GAAGACATGT TACAGAACAT CGCCCAGCCT GAATATCGAC AAATTGTTGT TGAGCTGCTA 
ATGGTTGTTT CTGTTATCCT GGAGAGAAAC CCGGAGTTTG AATTTCAAGA TAAAGTTGAT 
TTAGACAAAC TAGTCAAAGA GGCATTTAAT GATTTCCAGC AAGACCAGAG CAGACTTCAG 
GGAGCGGAGA AGCAGGATGA CATGACGATG TTTTACAATA CTCCCCCACT GGGCAAGCGA 
GGGACCTGCA GCTACCTGAC AAAAGCAGTG ATGAATATGC TGCTGGAAGC TGAAGTCAAG 
CCAAGTACTG AGGACCCTTG CATTGTTAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_012961648.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817102.1 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa09607
Clone ID Related Accession (Same CDS sequence)	XM_004914059.3 , XM_012961648.2
Accession Version	XM_012961648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012961648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GCTCCGTGTA TGAACCAATT AAGAGCTCGG ACCTACCCAG GCCAGACCAT 
GAAAGCCTGT GGGAAAAATT AGATCGGTAT TATAGGATTG TGAAAATCAC AGTGTTGAAT 
TATCAGAGTC CACAAACTGG TTTGTTCCCG ACCAAAACTG CTGGTGACTT TAAGAACGCG 
AAGATCCATG ACAGTCTATA CTGTGCTGCC AGCGCCTGGG CCCTCGCTCT TGCATACAGG 
CGCATTGATG ATGACAAGGG AAGAACCCAT GAGCTGGAGC ATTCTGCTAT AAAATGTATG 
AGAGGAATTC TGTACTGTTA CATGCGGCAG GCTGATAAGG TTGAGCAGTT TAAACAGGAT 
CCAAGTCCAC AAAAATGCCT TCATTCTATA TTCAATATAG ACAATGGAGA TGAGATATTA 
CCCTATAACG ATTATGGACA TATACAGATA AACGCAGTGT CACTTTTCCT TTTGTATCTT 
GTTGAGATGA TATCATCTGG ACTACAGATA ATCTACAATA CAGATGAGGT GTCGTTCATT 
CAGAACCTTG TATTCTGTGT AGAAAGAGCA TACCGTGTTC CTGACTTTGG AATCTGGGAA 
AGGGGCAGCA AGTATAACAA TGGCAGCACA GAATTACATT CCAGTTCTGT TGGTTTGGCT 
AAAGCTGCCC TTGAAGCCAT TGATGGATTC AACCTTTTTG GAAACCAGGG CTGCTCTTGG 
TCTGTCATCT TTGTGGATCT TGATGCTCAT AACAGGAACA GGCAAACTCT GAGTTCATTG 
CTTCCCAGAG AGTCCCGATC CCATAACACA GATGCAGCCC TCCTTACCTG TATCAGTTAC 
CCTGCATTTG CTGTTGATGA TGATGCTCTG TTTAACCAAA CACTGGATAA AATAGAACGT 
AAGCTTAAAG GAAAATATGG CTTTAAGCGA TTTCTAAGGG ATGGTTATCG GACTGCACTA 
GAAGATGAAA ACAGACGGTA CTACAAACCT GCTGAAATCA AGCTATTTGA CGGAATTGAA 
TGTGAATTCC CTTTGTTTTT TATCTACATG ATCATTGATG GTGTGTTCAG AGGGAAAGCT 
GAGCAAGTGA AAGAATATGA AGCACTCTTA GATCCTGTGC TATTTTATGG ACTTGATGGC 
TGTCCCGTGG TTCCAAAATA TTACTATGTG CCTGCTGATT TTGTTGAAGC AGAGAAGATG 
AACCCAAACA GCCAAAAGAG ATTTCCTAGC AACTGTGGGA GAGATGGAAA ACTCTTTCTC 
TGGGGACAAG CTCTGTACAT TATTGCTAAA CTGCTGGCCG AAGAATTAAT AAGTCCAAAA 
GACATTGATC CAATGAACCG TTTTGTTCAT CTTCGGGATC AGCGAAACAT CAGAATGCGG 
TACTCTAACC AGGGCCCCTT AGAAAATGAT GCCATGATAC ATGTGGCACT GATAGCTGAA 
AGCCAACGAT TGCAGGTTTT TCTCAACACT TATGGCATCC AAACCCAGAC CCCACAGCAA 
GTGGAACCTA TTCAGATATG GCCTCAGCAA GAACTTGTGA AGGCTTATCT TCACTTAGGA 
GTAAATAACA AGTTGGGGCT TTCTGGAAGG CCTGACAGGC CAATTGGATG CCTTGGCACA 
TCAAAGATTT ATCGTATTCT TGGGAAGACC GTTGTTTCCT ACCCTATTGT CTTTGATTTA 
AGTGACTTTT ACATGTCACA GGATGTGATG CTTCTGATCG ATGACATACA GAATGCTTTG 
CAGTTCATTA GGCAGTATTG GAAAATGAAT GGACGTCCCT TGTTTTTGGT TCTCATTCGG 
GAAGATAACA TCAGGGGAAG TCGATTTAAT CCAATCTTAG ACATGTTGGC TTCCTTTAAG 
AATGGAAATG TGGCCGGTGT GAAGGTTCAT GTGGACAGAC TGCAGACCTT AATATCCGGT 
GCAGTGGTTG AGCAGCTGGA TTTCTTGAGG ATCAATGAGA CAGAGGAGGT GCCTGAATTC 
AAAAGTTTTG AAGAGCTTGA AGTACCGAAA CATTCAAAGG TTAACAGGCA AGCAAGCATC 
CCTATTGCAG ATATGGAGCA TCAGCCAGAA ATAAACATGG GAGAATGGAA ACTCAAACCA 
ACCTATGAGG TTCTCGAGAA ACTAAATGAC TGCACCTGCC TGGCAAGCCA GGCCATCTTA 
CTAAGTATCT TACTGAAGAG AGAAGGACCT CATTTTATAA CCAAGGAAGG TACTGTCTCT 
GATTTTATGG CAAGAATCTA TAGAAAAGCT GGAAGCAATA AACTGTGGTC GGTTGTACGT 
CTTGCTGCCA GTCTTTTAGG TAAAATAGTG GACAGCCTTG CACCCTCTAT AACTAATGTT 
TTAGTTCAGG GCAAACAGGT AACACTGGGA GTCTTTGGCC ACGAGGAAGC GGTTATTTCC 
AATCCCTTGT CTCCAGGAGT GATCAAGGAT ATCCTCTACA AAAAGTGCAG CCCCTATGAT 
GAGCGAGAGG CTGTGCTGCA ACAAGAGCTA GTTATTCACA TTGGCTGGAT TATCTCCAAC 
ACACCAGAGC TTTTCAGCGG AATGTTAAAG ATACGGGTCG GATGGATTGT GCATGCAATG 
AGGTATGAAC TGAAGATCCG CAGTGGAGAT CAGCTAGAAA GGGACATCTA CCAGCTGTCT 
CCAGGAGAAA TCAAAGAGCT TCTCCTGTAC ATACTTCAGC CACAGCAGCA AGGAAGATGT 
TGGCTTCATC GAAGGCAGTT AGATGGATCC CTAAACCGAA ACCCATCTGG CTTCTACGAC 
AGAGTCTGGC AGATCCTGGA GAGGACACCC AGTGGTATCA TAGTTGCTGG CAAATTTCTA 
CCCCAACAAC CAACTCTGTC TGACATGACA ATGTATGAGA TGAACTTCTC CCTTCTGGTA 
GAAGACATGT TACAGAACAT CGCCCAGCCT GAATATCGAC AAATTGTTGT TGAGCTGCTA 
ATGGTTGTTT CTGTTATCCT GGAGAGAAAC CCGGAGTTTG AATTTCAAGA TAAAGTTGAT 
TTAGACAAAC TAGTCAAAGA GGCATTTAAT GATTTCCAGC AAGACCAGAG CAGACTTCAG 
GGAGCGGAGA AGCAGGATGA CATGACGATG TTTTACAATA CTCCCCCACT GGGCAAGCGA 
GGGACCTGCA GCTACCTGAC AAAAGCAGTG ATGAATATGC TGCTGGAAGC TGAAGTCAAG 
CCAAGTACTG AGGACCCTTG CATTGTTAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817102.1
CDS	112..3384
Translation	MQPIEMANSVDKAVSFSPTFLRSGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILPYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_012961648.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012961648.2

ORF Insert Sequence:

ATGCAACCAA TTGAAATGGC CAATTCTGTC GACAAGGCGG TTTCCTTCTC GCCAACATTC 
TTAAGGTCTG GCTCCGTGTA TGAACCAATT AAGAGCTCGG ACCTACCCAG GCCAGACCAT 
GAAAGCCTGT GGGAAAAATT AGATCGGTAT TATAGGATTG TGAAAATCAC AGTGTTGAAT 
TATCAGAGTC CACAAACTGG TTTGTTCCCG ACCAAAACTG CTGGTGACTT TAAGAACGCG 
AAGATCCATG ACAGTCTATA CTGTGCTGCC AGCGCCTGGG CCCTCGCTCT TGCATACAGG 
CGCATTGATG ATGACAAGGG AAGAACCCAT GAGCTGGAGC ATTCTGCTAT AAAATGTATG 
AGAGGAATTC TGTACTGTTA CATGCGGCAG GCTGATAAGG TTGAGCAGTT TAAACAGGAT 
CCAAGTCCAC AAAAATGCCT TCATTCTATA TTCAATATAG ACAATGGAGA TGAGATATTA 
CCCTATAACG ATTATGGACA TATACAGATA AACGCAGTGT CACTTTTCCT TTTGTATCTT 
GTTGAGATGA TATCATCTGG ACTACAGATA ATCTACAATA CAGATGAGGT GTCGTTCATT 
CAGAACCTTG TATTCTGTGT AGAAAGAGCA TACCGTGTTC CTGACTTTGG AATCTGGGAA 
AGGGGCAGCA AGTATAACAA TGGCAGCACA GAATTACATT CCAGTTCTGT TGGTTTGGCT 
AAAGCTGCCC TTGAAGCCAT TGATGGATTC AACCTTTTTG GAAACCAGGG CTGCTCTTGG 
TCTGTCATCT TTGTGGATCT TGATGCTCAT AACAGGAACA GGCAAACTCT GAGTTCATTG 
CTTCCCAGAG AGTCCCGATC CCATAACACA GATGCAGCCC TCCTTACCTG TATCAGTTAC 
CCTGCATTTG CTGTTGATGA TGATGCTCTG TTTAACCAAA CACTGGATAA AATAGAACGT 
AAGCTTAAAG GAAAATATGG CTTTAAGCGA TTTCTAAGGG ATGGTTATCG GACTGCACTA 
GAAGATGAAA ACAGACGGTA CTACAAACCT GCTGAAATCA AGCTATTTGA CGGAATTGAA 
TGTGAATTCC CTTTGTTTTT TATCTACATG ATCATTGATG GTGTGTTCAG AGGGAAAGCT 
GAGCAAGTGA AAGAATATGA AGCACTCTTA GATCCTGTGC TATTTTATGG ACTTGATGGC 
TGTCCCGTGG TTCCAAAATA TTACTATGTG CCTGCTGATT TTGTTGAAGC AGAGAAGATG 
AACCCAAACA GCCAAAAGAG ATTTCCTAGC AACTGTGGGA GAGATGGAAA ACTCTTTCTC 
TGGGGACAAG CTCTGTACAT TATTGCTAAA CTGCTGGCCG AAGAATTAAT AAGTCCAAAA 
GACATTGATC CAATGAACCG TTTTGTTCAT CTTCGGGATC AGCGAAACAT CAGAATGCGG 
TACTCTAACC AGGGCCCCTT AGAAAATGAT GCCATGATAC ATGTGGCACT GATAGCTGAA 
AGCCAACGAT TGCAGGTTTT TCTCAACACT TATGGCATCC AAACCCAGAC CCCACAGCAA 
GTGGAACCTA TTCAGATATG GCCTCAGCAA GAACTTGTGA AGGCTTATCT TCACTTAGGA 
GTAAATAACA AGTTGGGGCT TTCTGGAAGG CCTGACAGGC CAATTGGATG CCTTGGCACA 
TCAAAGATTT ATCGTATTCT TGGGAAGACC GTTGTTTCCT ACCCTATTGT CTTTGATTTA 
AGTGACTTTT ACATGTCACA GGATGTGATG CTTCTGATCG ATGACATACA GAATGCTTTG 
CAGTTCATTA GGCAGTATTG GAAAATGAAT GGACGTCCCT TGTTTTTGGT TCTCATTCGG 
GAAGATAACA TCAGGGGAAG TCGATTTAAT CCAATCTTAG ACATGTTGGC TTCCTTTAAG 
AATGGAAATG TGGCCGGTGT GAAGGTTCAT GTGGACAGAC TGCAGACCTT AATATCCGGT 
GCAGTGGTTG AGCAGCTGGA TTTCTTGAGG ATCAATGAGA CAGAGGAGGT GCCTGAATTC 
AAAAGTTTTG AAGAGCTTGA AGTACCGAAA CATTCAAAGG TTAACAGGCA AGCAAGCATC 
CCTATTGCAG ATATGGAGCA TCAGCCAGAA ATAAACATGG GAGAATGGAA ACTCAAACCA 
ACCTATGAGG TTCTCGAGAA ACTAAATGAC TGCACCTGCC TGGCAAGCCA GGCCATCTTA 
CTAAGTATCT TACTGAAGAG AGAAGGACCT CATTTTATAA CCAAGGAAGG TACTGTCTCT 
GATTTTATGG CAAGAATCTA TAGAAAAGCT GGAAGCAATA AACTGTGGTC GGTTGTACGT 
CTTGCTGCCA GTCTTTTAGG TAAAATAGTG GACAGCCTTG CACCCTCTAT AACTAATGTT 
TTAGTTCAGG GCAAACAGGT AACACTGGGA GTCTTTGGCC ACGAGGAAGC GGTTATTTCC 
AATCCCTTGT CTCCAGGAGT GATCAAGGAT ATCCTCTACA AAAAGTGCAG CCCCTATGAT 
GAGCGAGAGG CTGTGCTGCA ACAAGAGCTA GTTATTCACA TTGGCTGGAT TATCTCCAAC 
ACACCAGAGC TTTTCAGCGG AATGTTAAAG ATACGGGTCG GATGGATTGT GCATGCAATG 
AGGTATGAAC TGAAGATCCG CAGTGGAGAT CAGCTAGAAA GGGACATCTA CCAGCTGTCT 
CCAGGAGAAA TCAAAGAGCT TCTCCTGTAC ATACTTCAGC CACAGCAGCA AGGAAGATGT 
TGGCTTCATC GAAGGCAGTT AGATGGATCC CTAAACCGAA ACCCATCTGG CTTCTACGAC 
AGAGTCTGGC AGATCCTGGA GAGGACACCC AGTGGTATCA TAGTTGCTGG CAAATTTCTA 
CCCCAACAAC CAACTCTGTC TGACATGACA ATGTATGAGA TGAACTTCTC CCTTCTGGTA 
GAAGACATGT TACAGAACAT CGCCCAGCCT GAATATCGAC AAATTGTTGT TGAGCTGCTA 
ATGGTTGTTT CTGTTATCCT GGAGAGAAAC CCGGAGTTTG AATTTCAAGA TAAAGTTGAT 
TTAGACAAAC TAGTCAAAGA GGCATTTAAT GATTTCCAGC AAGACCAGAG CAGACTTCAG 
GGAGCGGAGA AGCAGGATGA CATGACGATG TTTTACAATA CTCCCCCACT GGGCAAGCGA 
GGGACCTGCA GCTACCTGAC AAAAGCAGTG ATGAATATGC TGCTGGAAGC TGAAGTCAAG 
CCAAGTACTG AGGACCCTTG CATTGTTAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phkb ( XM_004914060.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914117.1 Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa09608
Clone ID Related Accession (Same CDS sequence)	XM_004914060.3
Accession Version	XM_004914060.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	phosphorylase b kinase regulatory subunit beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914060.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGG TCCTGGCCGA TGTTAGCTGG AAGGTGCTCG AGTTAAGGGC CAGATCTAAA 
CGCTCAGGCT CCGTGTATGA ACCAATTAAG AGCTCGGACC TACCCAGGCC AGACCATGAA 
AGCCTGTGGG AAAAATTAGA TCGGTATTAT AGGATTGTGA AAATCACAGT GTTGAATTAT 
CAGAGTCCAC AAACTGGTTT GTTCCCGACC AAAACTGCTG GTGACTTTAA GAACGCGAAG 
ATCCATGACA GTCTATACTG TGCTGCCAGC GCCTGGGCCC TCGCTCTTGC ATACAGGCGC 
ATTGATGATG ACAAGGGAAG AACCCATGAG CTGGAGCATT CTGCTATAAA ATGTATGAGA 
GGAATTCTGT ACTGTTACAT GCGGCAGGCT GATAAGGTTG AGCAGTTTAA ACAGGATCCA 
AGTCCACAAA AATGCCTTCA TTCTATATTC AATATAGACA ATGGAGATGA GATATTACCC 
TATAACGATT ATGGACATAT ACAGATAAAC GCAGTGTCAC TTTTCCTTTT GTATCTTGTT 
GAGATGATAT CATCTGGACT ACAGATAATC TACAATACAG ATGAGGTGTC GTTCATTCAG 
AACCTTGTAT TCTGTGTAGA AAGAGCATAC CGTGTTCCTG ACTTTGGAAT CTGGGAAAGG 
GGCAGCAAGT ATAACAATGG CAGCACAGAA TTACATTCCA GTTCTGTTGG TTTGGCTAAA 
GCTGCCCTTG AAGCCATTGA TGGATTCAAC CTTTTTGGAA ACCAGGGCTG CTCTTGGTCT 
GTCATCTTTG TGGATCTTGA TGCTCATAAC AGGAACAGGC AAACTCTGAG TTCATTGCTT 
CCCAGAGAGT CCCGATCCCA TAACACAGAT GCAGCCCTCC TTACCTGTAT CAGTTACCCT 
GCATTTGCTG TTGATGATGA TGCTCTGTTT AACCAAACAC TGGATAAAAT AGAACGTAAG 
CTTAAAGGAA AATATGGCTT TAAGCGATTT CTAAGGGATG GTTATCGGAC TGCACTAGAA 
GATGAAAACA GACGGTACTA CAAACCTGCT GAAATCAAGC TATTTGACGG AATTGAATGT 
GAATTCCCTT TGTTTTTTAT CTACATGATC ATTGATGGTG TGTTCAGAGG GAAAGCTGAG 
CAAGTGAAAG AATATGAAGC ACTCTTAGAT CCTGTGCTAT TTTATGGACT TGATGGCTGT 
CCCGTGGTTC CAAAATATTA CTATGTGCCT GCTGATTTTG TTGAAGCAGA GAAGATGAAC 
CCAAACAGCC AAAAGAGATT TCCTAGCAAC TGTGGGAGAG ATGGAAAACT CTTTCTCTGG 
GGACAAGCTC TGTACATTAT TGCTAAACTG CTGGCCGAAG AATTAATAAG TCCAAAAGAC 
ATTGATCCAA TGAACCGTTT TGTTCATCTT CGGGATCAGC GAAACATCAG AATGCGGTAC 
TCTAACCAGG GCCCCTTAGA AAATGATGCC ATGATACATG TGGCACTGAT AGCTGAAAGC 
CAACGATTGC AGGTTTTTCT CAACACTTAT GGCATCCAAA CCCAGACCCC ACAGCAAGTG 
GAACCTATTC AGATATGGCC TCAGCAAGAA CTTGTGAAGG CTTATCTTCA CTTAGGAGTA 
AATAACAAGT TGGGGCTTTC TGGAAGGCCT GACAGGCCAA TTGGATGCCT TGGCACATCA 
AAGATTTATC GTATTCTTGG GAAGACCGTT GTTTCCTACC CTATTGTCTT TGATTTAAGT 
GACTTTTACA TGTCACAGGA TGTGATGCTT CTGATCGATG ACATACAGAA TGCTTTGCAG 
TTCATTAGGC AGTATTGGAA AATGAATGGA CGTCCCTTGT TTTTGGTTCT CATTCGGGAA 
GATAACATCA GGGGAAGTCG ATTTAATCCA ATCTTAGACA TGTTGGCTTC CTTTAAGAAT 
GGAAATGTGG CCGGTGTGAA GGTTCATGTG GACAGACTGC AGACCTTAAT ATCCGGTGCA 
GTGGTTGAGC AGCTGGATTT CTTGAGGATC AATGAGACAG AGGAGGTGCC TGAATTCAAA 
AGTTTTGAAG AGCTTGAAGT ACCGAAACAT TCAAAGGTTA ACAGGCAAGC AAGCATCCCT 
ATTGCAGATA TGGAGCATCA GCCAGAAATA AACATGGGAG AATGGAAACT CAAACCAACC 
TATGAGGTTC TCGAGAAACT AAATGACTGC ACCTGCCTGG CAAGCCAGGC CATCTTACTA 
AGTATCTTAC TGAAGAGAGA AGGACCTCAT TTTATAACCA AGGAAGGTAC TGTCTCTGAT 
TTTATGGCAA GAATCTATAG AAAAGCTGGA AGCAATAAAC TGTGGTCGGT TGTACGTCTT 
GCTGCCAGTC TTTTAGGTAA AATAGTGGAC AGCCTTGCAC CCTCTATAAC TAATGTTTTA 
GTTCAGGGCA AACAGGTAAC ACTGGGAGTC TTTGGCCACG AGGAAGCGGT TATTTCCAAT 
CCCTTGTCTC CAGGAGTGAT CAAGGATATC CTCTACAAAA AGTGCAGCCC CTATGATGAG 
CGAGAGGCTG TGCTGCAACA AGAGCTAGTT ATTCACATTG GCTGGATTAT CTCCAACACA 
CCAGAGCTTT TCAGCGGAAT GTTAAAGATA CGGGTCGGAT GGATTGTGCA TGCAATGAGG 
TATGAACTGA AGATCCGCAG TGGAGATCAG CTAGAAAGGG ACATCTACCA GCTGTCTCCA 
GGAGAAATCA AAGAGCTTCT CCTGTACATA CTTCAGCCAC AGCAGCAAGG AAGATGTTGG 
CTTCATCGAA GGCAGTTAGA TGGATCCCTA AACCGAAACC CATCTGGCTT CTACGACAGA 
GTCTGGCAGA TCCTGGAGAG GACACCCAGT GGTATCATAG TTGCTGGCAA ATTTCTACCC 
CAACAACCAA CTCTGTCTGA CATGACAATG TATGAGATGA ACTTCTCCCT TCTGGTAGAA 
GACATGTTAC AGAACATCGC CCAGCCTGAA TATCGACAAA TTGTTGTTGA GCTGCTAATG 
GTTGTTTCTG TTATCCTGGA GAGAAACCCG GAGTTTGAAT TTCAAGATAA AGTTGATTTA 
GACAAACTAG TCAAAGAGGC ATTTAATGAT TTCCAGCAAG ACCAGAGCAG ACTTCAGGGA 
GCGGAGAAGC AGGATGACAT GACGATGTTT TACAATACTC CCCCACTGGG CAAGCGAGGG 
ACCTGCAGCT ACCTGACAAA AGCAGTGATG AATATGCTGC TGGAAGCTGA AGTCAAGCCA 
AGTACTGAGG ACCCTTGCAT TGTTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914117.1
CDS	63..3332
Translation	MAQVLADVSWKVLELRARSKRSGSVYEPIKSSDLPRPDHESLWEKLDRYYRIVKITVLNYQSPQTGLFPTKTAGDFKNAKIHDSLYCAASAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVEQFKQDPSPQKCLHSIFNIDNGDEILPYNDYGHIQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERAYRVPDFGIWERGSKYNNGSTELHSSSVGLAKAALEAIDGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLTCISYPAFAVDDDALFNQTLDKIERKLKGKYGFKRFLRDGYRTALEDENRRYYKPAEIKLFDGIECEFPLFFIYMIIDGVFRGKAEQVKEYEALLDPVLFYGLDGCPVVPKYYYVPADFVEAEKMNPNSQKRFPSNCGRDGKLFLWGQALYIIAKLLAEELISPKDIDPMNRFVHLRDQRNIRMRYSNQGPLENDAMIHVALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLHLGVNNKLGLSGRPDRPIGCLGTSKIYRILGKTVVSYPIVFDLSDFYMSQDVMLLIDDIQNALQFIRQYWKMNGRPLFLVLIREDNIRGSRFNPILDMLASFKNGNVAGVKVHVDRLQTLISGAVVEQLDFLRINETEEVPEFKSFEELEVPKHSKVNRQASIPIADMEHQPEINMGEWKLKPTYEVLEKLNDCTCLASQAILLSILLKREGPHFITKEGTVSDFMARIYRKAGSNKLWSVVRLAASLLGKIVDSLAPSITNVLVQGKQVTLGVFGHEEAVISNPLSPGVIKDILYKKCSPYDEREAVLQQELVIHIGWIISNTPELFSGMLKIRVGWIVHAMRYELKIRSGDQLERDIYQLSPGEIKELLLYILQPQQQGRCWLHRRQLDGSLNRNPSGFYDRVWQILERTPSGIIVAGKFLPQQPTLSDMTMYEMNFSLLVEDMLQNIAQPEYRQIVVELLMVVSVILERNPEFEFQDKVDLDKLVKEAFNDFQQDQSRLQGAEKQDDMTMFYNTPPLGKRGTCSYLTKAVMNMLLEAEVKPSTEDPCIVS

Target ORF information:

RefSeq Version	XM_004914060.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphorylase kinase, beta (phkb), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914060.3

ORF Insert Sequence:

ATGGCTCAGG TCCTGGCCGA TGTTAGCTGG AAGGTGCTCG AGTTAAGGGC CAGATCTAAA 
CGCTCAGGCT CCGTGTATGA ACCAATTAAG AGCTCGGACC TACCCAGGCC AGACCATGAA 
AGCCTGTGGG AAAAATTAGA TCGGTATTAT AGGATTGTGA AAATCACAGT GTTGAATTAT 
CAGAGTCCAC AAACTGGTTT GTTCCCGACC AAAACTGCTG GTGACTTTAA GAACGCGAAG 
ATCCATGACA GTCTATACTG TGCTGCCAGC GCCTGGGCCC TCGCTCTTGC ATACAGGCGC 
ATTGATGATG ACAAGGGAAG AACCCATGAG CTGGAGCATT CTGCTATAAA ATGTATGAGA 
GGAATTCTGT ACTGTTACAT GCGGCAGGCT GATAAGGTTG AGCAGTTTAA ACAGGATCCA 
AGTCCACAAA AATGCCTTCA TTCTATATTC AATATAGACA ATGGAGATGA GATATTACCC 
TATAACGATT ATGGACATAT ACAGATAAAC GCAGTGTCAC TTTTCCTTTT GTATCTTGTT 
GAGATGATAT CATCTGGACT ACAGATAATC TACAATACAG ATGAGGTGTC GTTCATTCAG 
AACCTTGTAT TCTGTGTAGA AAGAGCATAC CGTGTTCCTG ACTTTGGAAT CTGGGAAAGG 
GGCAGCAAGT ATAACAATGG CAGCACAGAA TTACATTCCA GTTCTGTTGG TTTGGCTAAA 
GCTGCCCTTG AAGCCATTGA TGGATTCAAC CTTTTTGGAA ACCAGGGCTG CTCTTGGTCT 
GTCATCTTTG TGGATCTTGA TGCTCATAAC AGGAACAGGC AAACTCTGAG TTCATTGCTT 
CCCAGAGAGT CCCGATCCCA TAACACAGAT GCAGCCCTCC TTACCTGTAT CAGTTACCCT 
GCATTTGCTG TTGATGATGA TGCTCTGTTT AACCAAACAC TGGATAAAAT AGAACGTAAG 
CTTAAAGGAA AATATGGCTT TAAGCGATTT CTAAGGGATG GTTATCGGAC TGCACTAGAA 
GATGAAAACA GACGGTACTA CAAACCTGCT GAAATCAAGC TATTTGACGG AATTGAATGT 
GAATTCCCTT TGTTTTTTAT CTACATGATC ATTGATGGTG TGTTCAGAGG GAAAGCTGAG 
CAAGTGAAAG AATATGAAGC ACTCTTAGAT CCTGTGCTAT TTTATGGACT TGATGGCTGT 
CCCGTGGTTC CAAAATATTA CTATGTGCCT GCTGATTTTG TTGAAGCAGA GAAGATGAAC 
CCAAACAGCC AAAAGAGATT TCCTAGCAAC TGTGGGAGAG ATGGAAAACT CTTTCTCTGG 
GGACAAGCTC TGTACATTAT TGCTAAACTG CTGGCCGAAG AATTAATAAG TCCAAAAGAC 
ATTGATCCAA TGAACCGTTT TGTTCATCTT CGGGATCAGC GAAACATCAG AATGCGGTAC 
TCTAACCAGG GCCCCTTAGA AAATGATGCC ATGATACATG TGGCACTGAT AGCTGAAAGC 
CAACGATTGC AGGTTTTTCT CAACACTTAT GGCATCCAAA CCCAGACCCC ACAGCAAGTG 
GAACCTATTC AGATATGGCC TCAGCAAGAA CTTGTGAAGG CTTATCTTCA CTTAGGAGTA 
AATAACAAGT TGGGGCTTTC TGGAAGGCCT GACAGGCCAA TTGGATGCCT TGGCACATCA 
AAGATTTATC GTATTCTTGG GAAGACCGTT GTTTCCTACC CTATTGTCTT TGATTTAAGT 
GACTTTTACA TGTCACAGGA TGTGATGCTT CTGATCGATG ACATACAGAA TGCTTTGCAG 
TTCATTAGGC AGTATTGGAA AATGAATGGA CGTCCCTTGT TTTTGGTTCT CATTCGGGAA 
GATAACATCA GGGGAAGTCG ATTTAATCCA ATCTTAGACA TGTTGGCTTC CTTTAAGAAT 
GGAAATGTGG CCGGTGTGAA GGTTCATGTG GACAGACTGC AGACCTTAAT ATCCGGTGCA 
GTGGTTGAGC AGCTGGATTT CTTGAGGATC AATGAGACAG AGGAGGTGCC TGAATTCAAA 
AGTTTTGAAG AGCTTGAAGT ACCGAAACAT TCAAAGGTTA ACAGGCAAGC AAGCATCCCT 
ATTGCAGATA TGGAGCATCA GCCAGAAATA AACATGGGAG AATGGAAACT CAAACCAACC 
TATGAGGTTC TCGAGAAACT AAATGACTGC ACCTGCCTGG CAAGCCAGGC CATCTTACTA 
AGTATCTTAC TGAAGAGAGA AGGACCTCAT TTTATAACCA AGGAAGGTAC TGTCTCTGAT 
TTTATGGCAA GAATCTATAG AAAAGCTGGA AGCAATAAAC TGTGGTCGGT TGTACGTCTT 
GCTGCCAGTC TTTTAGGTAA AATAGTGGAC AGCCTTGCAC CCTCTATAAC TAATGTTTTA 
GTTCAGGGCA AACAGGTAAC ACTGGGAGTC TTTGGCCACG AGGAAGCGGT TATTTCCAAT 
CCCTTGTCTC CAGGAGTGAT CAAGGATATC CTCTACAAAA AGTGCAGCCC CTATGATGAG 
CGAGAGGCTG TGCTGCAACA AGAGCTAGTT ATTCACATTG GCTGGATTAT CTCCAACACA 
CCAGAGCTTT TCAGCGGAAT GTTAAAGATA CGGGTCGGAT GGATTGTGCA TGCAATGAGG 
TATGAACTGA AGATCCGCAG TGGAGATCAG CTAGAAAGGG ACATCTACCA GCTGTCTCCA 
GGAGAAATCA AAGAGCTTCT CCTGTACATA CTTCAGCCAC AGCAGCAAGG AAGATGTTGG 
CTTCATCGAA GGCAGTTAGA TGGATCCCTA AACCGAAACC CATCTGGCTT CTACGACAGA 
GTCTGGCAGA TCCTGGAGAG GACACCCAGT GGTATCATAG TTGCTGGCAA ATTTCTACCC 
CAACAACCAA CTCTGTCTGA CATGACAATG TATGAGATGA ACTTCTCCCT TCTGGTAGAA 
GACATGTTAC AGAACATCGC CCAGCCTGAA TATCGACAAA TTGTTGTTGA GCTGCTAATG 
GTTGTTTCTG TTATCCTGGA GAGAAACCCG GAGTTTGAAT TTCAAGATAA AGTTGATTTA 
GACAAACTAG TCAAAGAGGC ATTTAATGAT TTCCAGCAAG ACCAGAGCAG ACTTCAGGGA 
GCGGAGAAGC AGGATGACAT GACGATGTTT TACAATACTC CCCCACTGGG CAAGCGAGGG 
ACCTGCAGCT ACCTGACAAA AGCAGTGATG AATATGCTGC TGGAAGCTGA AGTCAAGCCA 
AGTACTGAGG ACCCTTGCAT TGTTAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	phkb
Entrez Gene ID	100493974
Full Name	phosphorylase kinase, beta
General protein information	Preferred Names phosphorylase kinase, beta Names phosphorylase b kinase regulatory subunit beta
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Homo sapiens (human)	PHKB	NP_000284.1
Caenorhabditis elegans (roundworm)	Y67D8A.2	NP_741355.3
Xenopus tropicalis (tropical clawed frog)	phkb	XP_004914116.1
	PHKB	XP_001113858.1
Danio rerio (zebrafish)	phkb	XP_698197.5
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009278	XP_320073.4
Canis lupus familiaris (dog)	PHKB	XP_005617652.1
Mus musculus (house mouse)	Phkb	NP_955517.1
Pan troglodytes (chimpanzee)	PHKB	XP_001162360.1
Bos taurus (cattle)	PHKB	XP_002694819.1
Rattus norvegicus (Norway rat)	Phkb	NP_001014174.1
Gallus gallus (chicken)	PHKB	NP_001007832.1
Drosophila melanogaster (fruit fly)	CG8475	NP_001260230.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

phkb cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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