rock2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following rock2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rock2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa10314 XM_004914468.3
Latest version!
Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa10315 XM_004914469.3
Latest version!
Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa10317 XM_002942181.4
Latest version!
Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa44819 XM_018094480.1
Latest version!
Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
View Old Accession Versions >>
OXa10317 XM_002942181.3 Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa10315 XM_004914469.2 Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa10314 XM_004914468.2 Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa10314
    Clone ID Related Accession (Same CDS sequence) XM_004914468.2 , XM_004914468.3
    Accession Version XM_004914468.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho-associated protein kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914468.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GCAGTCAGCG CACTTGCAAA AAGTCATTAA CAAGCTGGAA
    GAGCAGCTCC ATAATGAGAT GCAAACCAAA GACGAACTGG AGCAAAAGTT CAGAGCTGTA
    AATTTACGTT TGGAGAAGAC AGTGAAGGAG CTGGATGAAG AGGCCAATGC TAGGAAGAGC
    ATAGAATCGA CCACCAGGCA GCTGGAGCGT GAGAAGGCTC TTCTGCAGCA CAAGAGCACA
    GAGTACCAAA GAAAAGCAGA GAATGATGCC GACAGGAAGC GCAGCCTGGA AAATGAAGTT
    AATAGTTTAA AGGATCAGCT TGAAGATTTG AAAAGAAGAA ACCAAAATTC CCAAATATCA
    AATGAGAAGA TGAATCAGCT CCAAAGACAG CTGGATGAAG CCAACGCACA GCTTCGCACA
    GAGTCTGATG CAGCTGCCCG GCTGAGGAAG ACTCAGACCG AGATGTCCAA ACAAATACAG
    CAGCTAGAAA CCAACAACCG TGAGCTTCAG GATAAGACTT GCATGTTGGA GAATGCCAAG
    CTGAAACTAG AAAAGGACTT TATTAACCTG CAGTCGGCTT TGGAGTCAGA ACGGAGGGAC
    CGGACTCAGG GGTCAGAAGT AATAAGTGAC CTTCAAGGAC GAATATCTGT CTTGGAAGAG
    GATCTAAAGA AAGGCAAAGA GCTGCTAGCA AGAGCAGAAG CAGAAAAGCA GCAAATGCAT
    GAGGGGTTAG CCATTTTGGA GAAGGAAAAG AGCAATCTGG AAATCGATAT GACCTACAAA
    CTAAAAGCAC TACAGCAAAG CGTTGAGAAA GAGGAGGCTG AACATAAAGC CACAAAAGCT
    CGGCTGGCTG ACAAGAACAA AATTTACCAG TCCATTGAGG AGACCAAGTC AGAAGCAATG
    AAAGACATGG AGAAGAAGCT TCATGAGGAG CGCCTGGTTA AGCAGAGGCT AGAGAACAGC
    TTGCTGGAGA CAGAGAAACA ATACTCCATG TTGGACTGTG ACCTCAAACA AGCCAAGCAA
    AAGATCAACG AGTTGGAATC ACTTAAAGAC AAACTTAGCG AGGATGTTAA AAACTTAACG
    CTCAAAGCAG AACAAGAGGC ACAGAAGCGC AACCTGACGC ATAACGATCT GAAGATGCAG
    ATACAGCAAG TGAATAGCCT GAAGATGTCT GAGAAGCAAC TGAAGCAAGA GATCAACCAT
    CTGACTGAGA TAAAACTTAA CCTGGAGAAA CAGAACAACG AGTTGCGCAA AGAAAGAGTA
    GATGCGGATG GACAGATGAA GGAGCTTCAA GACCAACTGG AGGCTGAGCA GTATTTCTCA
    ACGCTGTACA AGACGCAGGT GCGGGAGCTG AAAGAGGAGT GTGAAGACAA GAGCAAGATG
    TGCAAAGAAG TGCAGCAGAA GGTCCAAGAG CTTCAGGATG AGAGAGACTC CCTGGCGGCT
    CAACTGGAGA TCACATTGAC CAAGGCCGAC TCCGAGCAGC TGGCCCGTTC CATTGCCGAG
    GAACAGTATT CCGACCTGGA GAAAGAGAAG ATCATGAAGG AACTAGAGAT TAAGGAGATG
    ATGGCACGGC ACAAGCAGGA GCTGGCAGAA AAATATGCTA CAATTGCTTC TTTGGAAGAA
    GCTAACAAAA CACTGACTAT CGATGTAGGC AATTTGGCTA ATGAGAAGGA AGACTTAAAC
    AACAAACTGA AAGAAACCCA AGAACTAATT CAGAATCTGA AAGAGGAAGA AAGCAGCATT
    ATTACCGTCA AGGCACAGTT TGAGAAGCAG CTGTTAACTG AGAGGACTTT GAAGACACAG
    GCTGTAAACA AGTTAGCCGA AATCATGAAC CGCAAGTTAC CCACGAAGCG AGGGCCGGAC
    ACAGATGTAC GAAGAAAGGA AAAGGAGAAC CGCAAGCTGC AGTTGGACCT GAAATCGGAG
    CGTGAGAAAT TCTCTCAGCT TGTCTTCAAA TACCAGAGGG AAGTGAATGA CATGCAGGCG
    CTAATAGCAG ATGAGAACCA AGCCCGAGTC GAACTGCAGA TGGCGCTAGA TAGCAAAGAC
    AGTGACATAG AGCAGCTCCG GTCGCAACTG CAGTCTATGA ACCTCGGCCT TGACAGCACA
    AGTATCGGCA GTGGACACGG TGACACAGAT GCTGATGATG GCTTTCCAGA ATCCAGGCTG
    GAAGGATGGT TGTCGCTTCC TTTGCGCAAC GCCAAAAAGT TTGGTTGGAA TAAGAAGTAC
    GTAGTGGTGA GCAGCAGGAA GATCCTGTTT TATGACAGTG AGCAGGATAA GGAGCTGTCC
    AACCCTTCCA TGGTGCTGGA TATTGATAAG TTGTTCCATG TGCGACCAGT TACTCAGACG
    GATGTGTATC GAGCCGATGC CAAAGAAATT CCACGGATTT TCCAGATTCT ATACGCCAAT
    GAAGGAGAAA GCAAGAAAGA ACAAGAGTTC CAAGTGGACC CCTTAGAGAA ATCCAATTAC
    ATATGTCATA AAGGACATGA GTTTATCCCC ACACTCTACC ATTTCCCCAC CAGCTGTGAT
    GCTTGTATGA AGCCCCTGTG GCACATGTTC AAACCCCCTG CTGCCCTGGA GTGTCGGCGC
    TGCCATATCA AGTGCCACAA GGATCATATG GACAAGAAAG AAGAGATAAT AGCACCTTGC
    AAAGTGAACT ATGATATTTC CACCGCAAAG AATCTACTAT TACTAGCCAA CTCCACAGAA
    GAACAGCAGA AGTGGGTGAG TCGACTGGTG AAAAAGATCC CCAAGAAGCC CCCTGCTTCT
    GACCATCAAG CTCGGTCCTC CCCCCGGCCT CCTGCTAAGG CGTCGCTAAA CCAGTCCATG
    AGGCGGCCAA GCCGGCAGCT TCCTCCAAAT AAACCCAGAT TACTTGATCT GGCATTTAAA
    GACTGGGACT GGTCTCTTGA TGATGTAGAG GATGATGATG AGGATGTTTT TGATTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914525.1
    CDS976..5175
    Translation

    Target ORF information:

    RefSeq Version XM_004914468.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914468.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GCAGTCAGCG CACTTGCAAA AAGTCATTAA CAAGCTGGAA
    GAGCAGCTCC ATAATGAGAT GCAAACCAAA GACGAACTGG AGCAAAAGTT CAGAGCTGTA
    AATTTACGTT TGGAGAAGAC AGTGAAGGAG CTGGATGAAG AGGCCAATGC TAGGAAGAGC
    ATAGAATCGA CCACCAGGCA GCTGGAGCGT GAGAAGGCTC TTCTGCAGCA CAAGAGCACA
    GAGTACCAAA GAAAAGCAGA GAATGATGCC GACAGGAAGC GCAGCCTGGA AAATGAAGTT
    AATAGTTTAA AGGATCAGCT TGAAGATTTG AAAAGAAGAA ACCAAAATTC CCAAATATCA
    AATGAGAAGA TGAATCAGCT CCAAAGACAG CTGGATGAAG CCAACGCACA GCTTCGCACA
    GAGTCTGATG CAGCTGCCCG GCTGAGGAAG ACTCAGACCG AGATGTCCAA ACAAATACAG
    CAGCTAGAAA CCAACAACCG TGAGCTTCAG GATAAGACTT GCATGTTGGA GAATGCCAAG
    CTGAAACTAG AAAAGGACTT TATTAACCTG CAGTCGGCTT TGGAGTCAGA ACGGAGGGAC
    CGGACTCAGG GGTCAGAAGT AATAAGTGAC CTTCAAGGAC GAATATCTGT CTTGGAAGAG
    GATCTAAAGA AAGGCAAAGA GCTGCTAGCA AGAGCAGAAG CAGAAAAGCA GCAAATGCAT
    GAGGGGTTAG CCATTTTGGA GAAGGAAAAG AGCAATCTGG AAATCGATAT GACCTACAAA
    CTAAAAGCAC TACAGCAAAG CGTTGAGAAA GAGGAGGCTG AACATAAAGC CACAAAAGCT
    CGGCTGGCTG ACAAGAACAA AATTTACCAG TCCATTGAGG AGACCAAGTC AGAAGCAATG
    AAAGACATGG AGAAGAAGCT TCATGAGGAG CGCCTGGTTA AGCAGAGGCT AGAGAACAGC
    TTGCTGGAGA CAGAGAAACA ATACTCCATG TTGGACTGTG ACCTCAAACA AGCCAAGCAA
    AAGATCAACG AGTTGGAATC ACTTAAAGAC AAACTTAGCG AGGATGTTAA AAACTTAACG
    CTCAAAGCAG AACAAGAGGC ACAGAAGCGC AACCTGACGC ATAACGATCT GAAGATGCAG
    ATACAGCAAG TGAATAGCCT GAAGATGTCT GAGAAGCAAC TGAAGCAAGA GATCAACCAT
    CTGACTGAGA TAAAACTTAA CCTGGAGAAA CAGAACAACG AGTTGCGCAA AGAAAGAGTA
    GATGCGGATG GACAGATGAA GGAGCTTCAA GACCAACTGG AGGCTGAGCA GTATTTCTCA
    ACGCTGTACA AGACGCAGGT GCGGGAGCTG AAAGAGGAGT GTGAAGACAA GAGCAAGATG
    TGCAAAGAAG TGCAGCAGAA GGTCCAAGAG CTTCAGGATG AGAGAGACTC CCTGGCGGCT
    CAACTGGAGA TCACATTGAC CAAGGCCGAC TCCGAGCAGC TGGCCCGTTC CATTGCCGAG
    GAACAGTATT CCGACCTGGA GAAAGAGAAG ATCATGAAGG AACTAGAGAT TAAGGAGATG
    ATGGCACGGC ACAAGCAGGA GCTGGCAGAA AAATATGCTA CAATTGCTTC TTTGGAAGAA
    GCTAACAAAA CACTGACTAT CGATGTAGGC AATTTGGCTA ATGAGAAGGA AGACTTAAAC
    AACAAACTGA AAGAAACCCA AGAACTAATT CAGAATCTGA AAGAGGAAGA AAGCAGCATT
    ATTACCGTCA AGGCACAGTT TGAGAAGCAG CTGTTAACTG AGAGGACTTT GAAGACACAG
    GCTGTAAACA AGTTAGCCGA AATCATGAAC CGCAAGTTAC CCACGAAGCG AGGGCCGGAC
    ACAGATGTAC GAAGAAAGGA AAAGGAGAAC CGCAAGCTGC AGTTGGACCT GAAATCGGAG
    CGTGAGAAAT TCTCTCAGCT TGTCTTCAAA TACCAGAGGG AAGTGAATGA CATGCAGGCG
    CTAATAGCAG ATGAGAACCA AGCCCGAGTC GAACTGCAGA TGGCGCTAGA TAGCAAAGAC
    AGTGACATAG AGCAGCTCCG GTCGCAACTG CAGTCTATGA ACCTCGGCCT TGACAGCACA
    AGTATCGGCA GTGGACACGG TGACACAGAT GCTGATGATG GCTTTCCAGA ATCCAGGCTG
    GAAGGATGGT TGTCGCTTCC TTTGCGCAAC GCCAAAAAGT TTGGTTGGAA TAAGAAGTAC
    GTAGTGGTGA GCAGCAGGAA GATCCTGTTT TATGACAGTG AGCAGGATAA GGAGCTGTCC
    AACCCTTCCA TGGTGCTGGA TATTGATAAG TTGTTCCATG TGCGACCAGT TACTCAGACG
    GATGTGTATC GAGCCGATGC CAAAGAAATT CCACGGATTT TCCAGATTCT ATACGCCAAT
    GAAGGAGAAA GCAAGAAAGA ACAAGAGTTC CAAGTGGACC CCTTAGAGAA ATCCAATTAC
    ATATGTCATA AAGGACATGA GTTTATCCCC ACACTCTACC ATTTCCCCAC CAGCTGTGAT
    GCTTGTATGA AGCCCCTGTG GCACATGTTC AAACCCCCTG CTGCCCTGGA GTGTCGGCGC
    TGCCATATCA AGTGCCACAA GGATCATATG GACAAGAAAG AAGAGATAAT AGCACCTTGC
    AAAGTGAACT ATGATATTTC CACCGCAAAG AATCTACTAT TACTAGCCAA CTCCACAGAA
    GAACAGCAGA AGTGGGTGAG TCGACTGGTG AAAAAGATCC CCAAGAAGCC CCCTGCTTCT
    GACCATCAAG CTCGGTCCTC CCCCCGGCCT CCTGCTAAGG CGTCGCTAAA CCAGTCCATG
    AGGCGGCCAA GCCGGCAGCT TCCTCCAAAT AAACCCAGAT TACTTGATCT GGCATTTAAA
    GACTGGGACT GGTCTCTTGA TGATGTAGAG GATGATGATG AGGATGTTTT TGATTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10315
    Clone ID Related Accession (Same CDS sequence) XM_004914469.2 , XM_004914469.3
    Accession Version XM_004914469.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho-associated protein kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914469.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GTCAGCGCAC TTGCAAAAAG TCATTAACAA GCTGGAAGAG
    CAGCTCCATA ATGAGATGCA AACCAAAGAC GAACTGGAGC AAAAGTTCAG AGCTGTAAAT
    TTACGTTTGG AGAAGACAGT GAAGGAGCTG GATGAAGAGG CCAATGCTAG GAAGAGCATA
    GAATCGACCA CCAGGCAGCT GGAGCGTGAG AAGGCTCTTC TGCAGCACAA GAGCACAGAG
    TACCAAAGAA AAGCAGAGAA TGATGCCGAC AGGAAGCGCA GCCTGGAAAA TGAAGTTAAT
    AGTTTAAAGG ATCAGCTTGA AGATTTGAAA AGAAGAAACC AAAATTCCCA AATATCAAAT
    GAGAAGATGA ATCAGCTCCA AAGACAGCTG GATGAAGCCA ACGCACAGCT TCGCACAGAG
    TCTGATGCAG CTGCCCGGCT GAGGAAGACT CAGACCGAGA TGTCCAAACA AATACAGCAG
    CTAGAAACCA ACAACCGTGA GCTTCAGGAT AAGACTTGCA TGTTGGAGAA TGCCAAGCTG
    AAACTAGAAA AGGACTTTAT TAACCTGCAG TCGGCTTTGG AGTCAGAACG GAGGGACCGG
    ACTCAGGGGT CAGAAGTAAT AAGTGACCTT CAAGGACGAA TATCTGTCTT GGAAGAGGAT
    CTAAAGAAAG GCAAAGAGCT GCTAGCAAGA GCAGAAGCAG AAAAGCAGCA AATGCATGAG
    GGGTTAGCCA TTTTGGAGAA GGAAAAGAGC AATCTGGAAA TCGATATGAC CTACAAACTA
    AAAGCACTAC AGCAAAGCGT TGAGAAAGAG GAGGCTGAAC ATAAAGCCAC AAAAGCTCGG
    CTGGCTGACA AGAACAAAAT TTACCAGTCC ATTGAGGAGA CCAAGTCAGA AGCAATGAAA
    GACATGGAGA AGAAGCTTCA TGAGGAGCGC CTGGTTAAGC AGAGGCTAGA GAACAGCTTG
    CTGGAGACAG AGAAACAATA CTCCATGTTG GACTGTGACC TCAAACAAGC CAAGCAAAAG
    ATCAACGAGT TGGAATCACT TAAAGACAAA CTTAGCGAGG ATGTTAAAAA CTTAACGCTC
    AAAGCAGAAC AAGAGGCACA GAAGCGCAAC CTGACGCATA ACGATCTGAA GATGCAGATA
    CAGCAAGTGA ATAGCCTGAA GATGTCTGAG AAGCAACTGA AGCAAGAGAT CAACCATCTG
    ACTGAGATAA AACTTAACCT GGAGAAACAG AACAACGAGT TGCGCAAAGA AAGAGTAGAT
    GCGGATGGAC AGATGAAGGA GCTTCAAGAC CAACTGGAGG CTGAGCAGTA TTTCTCAACG
    CTGTACAAGA CGCAGGTGCG GGAGCTGAAA GAGGAGTGTG AAGACAAGAG CAAGATGTGC
    AAAGAAGTGC AGCAGAAGGT CCAAGAGCTT CAGGATGAGA GAGACTCCCT GGCGGCTCAA
    CTGGAGATCA CATTGACCAA GGCCGACTCC GAGCAGCTGG CCCGTTCCAT TGCCGAGGAA
    CAGTATTCCG ACCTGGAGAA AGAGAAGATC ATGAAGGAAC TAGAGATTAA GGAGATGATG
    GCACGGCACA AGCAGGAGCT GGCAGAAAAA TATGCTACAA TTGCTTCTTT GGAAGAAGCT
    AACAAAACAC TGACTATCGA TGTAGGCAAT TTGGCTAATG AGAAGGAAGA CTTAAACAAC
    AAACTGAAAG AAACCCAAGA ACTAATTCAG AATCTGAAAG AGGAAGAAAG CAGCATTATT
    ACCGTCAAGG CACAGTTTGA GAAGCAGCTG TTAACTGAGA GGACTTTGAA GACACAGGCT
    GTAAACAAGT TAGCCGAAAT CATGAACCGC AAGTTACCCA CGAAGCGAGG GCCGGACACA
    GATGTACGAA GAAAGGAAAA GGAGAACCGC AAGCTGCAGT TGGACCTGAA ATCGGAGCGT
    GAGAAATTCT CTCAGCTTGT CTTCAAATAC CAGAGGGAAG TGAATGACAT GCAGGCGCTA
    ATAGCAGATG AGAACCAAGC CCGAGTCGAA CTGCAGATGG CGCTAGATAG CAAAGACAGT
    GACATAGAGC AGCTCCGGTC GCAACTGCAG TCTATGAACC TCGGCCTTGA CAGCACAAGT
    ATCGGCAGTG GACACGGTGA CACAGATGCT GATGATGGCT TTCCAGAATC CAGGCTGGAA
    GGATGGTTGT CGCTTCCTTT GCGCAACGCC AAAAAGTTTG GTTGGAATAA GAAGTACGTA
    GTGGTGAGCA GCAGGAAGAT CCTGTTTTAT GACAGTGAGC AGGATAAGGA GCTGTCCAAC
    CCTTCCATGG TGCTGGATAT TGATAAGTTG TTCCATGTGC GACCAGTTAC TCAGACGGAT
    GTGTATCGAG CCGATGCCAA AGAAATTCCA CGGATTTTCC AGATTCTATA CGCCAATGAA
    GGAGAAAGCA AGAAAGAACA AGAGTTCCAA GTGGACCCCT TAGAGAAATC CAATTACATA
    TGTCATAAAG GACATGAGTT TATCCCCACA CTCTACCATT TCCCCACCAG CTGTGATGCT
    TGTATGAAGC CCCTGTGGCA CATGTTCAAA CCCCCTGCTG CCCTGGAGTG TCGGCGCTGC
    CATATCAAGT GCCACAAGGA TCATATGGAC AAGAAAGAAG AGATAATAGC ACCTTGCAAA
    GTGAACTATG ATATTTCCAC CGCAAAGAAT CTACTATTAC TAGCCAACTC CACAGAAGAA
    CAGCAGAAGT GGGTGAGTCG ACTGGTGAAA AAGATCCCCA AGAAGCCCCC TGCTTCTGAC
    CATCAAGCTC GGTCCTCCCC CCGGCCTCCT GCTAAGGCGT CGCTAAACCA GTCCATGAGG
    CGGCCAAGCC GGCAGCTTCC TCCAAATAAA CCCAGATTAC TTGATCTGGC ATTTAAAGAC
    TGGGACTGGT CTCTTGATGA TGTAGAGGAT GATGATGAGG ATGTTTTTGA TTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914526.1
    CDS976..5172
    Translation

    Target ORF information:

    RefSeq Version XM_004914469.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914469.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GTCAGCGCAC TTGCAAAAAG TCATTAACAA GCTGGAAGAG
    CAGCTCCATA ATGAGATGCA AACCAAAGAC GAACTGGAGC AAAAGTTCAG AGCTGTAAAT
    TTACGTTTGG AGAAGACAGT GAAGGAGCTG GATGAAGAGG CCAATGCTAG GAAGAGCATA
    GAATCGACCA CCAGGCAGCT GGAGCGTGAG AAGGCTCTTC TGCAGCACAA GAGCACAGAG
    TACCAAAGAA AAGCAGAGAA TGATGCCGAC AGGAAGCGCA GCCTGGAAAA TGAAGTTAAT
    AGTTTAAAGG ATCAGCTTGA AGATTTGAAA AGAAGAAACC AAAATTCCCA AATATCAAAT
    GAGAAGATGA ATCAGCTCCA AAGACAGCTG GATGAAGCCA ACGCACAGCT TCGCACAGAG
    TCTGATGCAG CTGCCCGGCT GAGGAAGACT CAGACCGAGA TGTCCAAACA AATACAGCAG
    CTAGAAACCA ACAACCGTGA GCTTCAGGAT AAGACTTGCA TGTTGGAGAA TGCCAAGCTG
    AAACTAGAAA AGGACTTTAT TAACCTGCAG TCGGCTTTGG AGTCAGAACG GAGGGACCGG
    ACTCAGGGGT CAGAAGTAAT AAGTGACCTT CAAGGACGAA TATCTGTCTT GGAAGAGGAT
    CTAAAGAAAG GCAAAGAGCT GCTAGCAAGA GCAGAAGCAG AAAAGCAGCA AATGCATGAG
    GGGTTAGCCA TTTTGGAGAA GGAAAAGAGC AATCTGGAAA TCGATATGAC CTACAAACTA
    AAAGCACTAC AGCAAAGCGT TGAGAAAGAG GAGGCTGAAC ATAAAGCCAC AAAAGCTCGG
    CTGGCTGACA AGAACAAAAT TTACCAGTCC ATTGAGGAGA CCAAGTCAGA AGCAATGAAA
    GACATGGAGA AGAAGCTTCA TGAGGAGCGC CTGGTTAAGC AGAGGCTAGA GAACAGCTTG
    CTGGAGACAG AGAAACAATA CTCCATGTTG GACTGTGACC TCAAACAAGC CAAGCAAAAG
    ATCAACGAGT TGGAATCACT TAAAGACAAA CTTAGCGAGG ATGTTAAAAA CTTAACGCTC
    AAAGCAGAAC AAGAGGCACA GAAGCGCAAC CTGACGCATA ACGATCTGAA GATGCAGATA
    CAGCAAGTGA ATAGCCTGAA GATGTCTGAG AAGCAACTGA AGCAAGAGAT CAACCATCTG
    ACTGAGATAA AACTTAACCT GGAGAAACAG AACAACGAGT TGCGCAAAGA AAGAGTAGAT
    GCGGATGGAC AGATGAAGGA GCTTCAAGAC CAACTGGAGG CTGAGCAGTA TTTCTCAACG
    CTGTACAAGA CGCAGGTGCG GGAGCTGAAA GAGGAGTGTG AAGACAAGAG CAAGATGTGC
    AAAGAAGTGC AGCAGAAGGT CCAAGAGCTT CAGGATGAGA GAGACTCCCT GGCGGCTCAA
    CTGGAGATCA CATTGACCAA GGCCGACTCC GAGCAGCTGG CCCGTTCCAT TGCCGAGGAA
    CAGTATTCCG ACCTGGAGAA AGAGAAGATC ATGAAGGAAC TAGAGATTAA GGAGATGATG
    GCACGGCACA AGCAGGAGCT GGCAGAAAAA TATGCTACAA TTGCTTCTTT GGAAGAAGCT
    AACAAAACAC TGACTATCGA TGTAGGCAAT TTGGCTAATG AGAAGGAAGA CTTAAACAAC
    AAACTGAAAG AAACCCAAGA ACTAATTCAG AATCTGAAAG AGGAAGAAAG CAGCATTATT
    ACCGTCAAGG CACAGTTTGA GAAGCAGCTG TTAACTGAGA GGACTTTGAA GACACAGGCT
    GTAAACAAGT TAGCCGAAAT CATGAACCGC AAGTTACCCA CGAAGCGAGG GCCGGACACA
    GATGTACGAA GAAAGGAAAA GGAGAACCGC AAGCTGCAGT TGGACCTGAA ATCGGAGCGT
    GAGAAATTCT CTCAGCTTGT CTTCAAATAC CAGAGGGAAG TGAATGACAT GCAGGCGCTA
    ATAGCAGATG AGAACCAAGC CCGAGTCGAA CTGCAGATGG CGCTAGATAG CAAAGACAGT
    GACATAGAGC AGCTCCGGTC GCAACTGCAG TCTATGAACC TCGGCCTTGA CAGCACAAGT
    ATCGGCAGTG GACACGGTGA CACAGATGCT GATGATGGCT TTCCAGAATC CAGGCTGGAA
    GGATGGTTGT CGCTTCCTTT GCGCAACGCC AAAAAGTTTG GTTGGAATAA GAAGTACGTA
    GTGGTGAGCA GCAGGAAGAT CCTGTTTTAT GACAGTGAGC AGGATAAGGA GCTGTCCAAC
    CCTTCCATGG TGCTGGATAT TGATAAGTTG TTCCATGTGC GACCAGTTAC TCAGACGGAT
    GTGTATCGAG CCGATGCCAA AGAAATTCCA CGGATTTTCC AGATTCTATA CGCCAATGAA
    GGAGAAAGCA AGAAAGAACA AGAGTTCCAA GTGGACCCCT TAGAGAAATC CAATTACATA
    TGTCATAAAG GACATGAGTT TATCCCCACA CTCTACCATT TCCCCACCAG CTGTGATGCT
    TGTATGAAGC CCCTGTGGCA CATGTTCAAA CCCCCTGCTG CCCTGGAGTG TCGGCGCTGC
    CATATCAAGT GCCACAAGGA TCATATGGAC AAGAAAGAAG AGATAATAGC ACCTTGCAAA
    GTGAACTATG ATATTTCCAC CGCAAAGAAT CTACTATTAC TAGCCAACTC CACAGAAGAA
    CAGCAGAAGT GGGTGAGTCG ACTGGTGAAA AAGATCCCCA AGAAGCCCCC TGCTTCTGAC
    CATCAAGCTC GGTCCTCCCC CCGGCCTCCT GCTAAGGCGT CGCTAAACCA GTCCATGAGG
    CGGCCAAGCC GGCAGCTTCC TCCAAATAAA CCCAGATTAC TTGATCTGGC ATTTAAAGAC
    TGGGACTGGT CTCTTGATGA TGTAGAGGAT GATGATGAGG ATGTTTTTGA TTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10317
    Clone ID Related Accession (Same CDS sequence) XM_002942181.3 , XM_002942181.4
    Accession Version XM_002942181.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4122bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho-associated protein kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002942181.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GCAGTCAGCG CACTTGCAAA AAGTCATTAA CAAGCTGGAA
    GAGCAGCTCC ATAATGAGAT GCAAACCAAA GACGAACTGG AGCAAAAGTT CAGAGCTGTA
    AATTTACGTT TGGAGAAGAC AGTGAAGGAG CTGGATGAAG AGGCCAATGC TAGGAAGAGC
    ATAGAATCGA CCACCAGGCA GCTGGAGCGT GAGAAGGCTC TTCTGCAGCA CAAGAGCACA
    GAGTACCAAA GAAAAGCAGA GAATGATGCC GACAGGAAGC GCAGCCTGGA AAATGAAGTT
    AATAGTTTAA AGGATCAGCT TGAAGATTTG AAAAGAAGAA ACCAAAATTC CCAAATATCA
    AATGAGAAGA TGAATCAGCT CCAAAGACAG CTGGATGAAG CCAACGCACA GCTTCGCACA
    GAGTCTGATG CAGCTGCCCG GCTGAGGAAG ACTCAGACCG AGATGTCCAA ACAAATACAG
    CAGCTAGAAA CCAACAACCG TGAGCTTCAG GATAAGACTT GCATGTTGGA GAATGCCAAG
    CTGAAACTAG AAAAGGACTT TATTAACCTG CAGTCGGCTT TGGAGTCAGA ACGGAGGGAC
    CGGACTCAGG GGTCAGAAGT AATAAGTGAC CTTCAAGGAC GAATATCTGT CTTGGAAGAG
    GATCTAAAGA AAGGCAAAGA GCTGCTAGCA AGAGCAGAAG CAGAAAAGCA GCAAATGCAT
    GAGGGGTTAG CCATTTTGGA GAAGGAAAAG AGCAATCTGG AAATCGATAT GACCTACAAA
    CTAAAAGCAC TACAGCAAAG CGTTGAGAAA GAGGAGGCTG AACATAAAGC CACAAAAGCT
    CGGCTGGCTG ACAAGAACAA AATTTACCAG TCCATTGAGG AGACCAAGTC AGAAGCAATG
    AAAGACATGG AGAAGAAGCT TCATGAGGAG CGCCTGGTTA AGCAGAGGCT AGAGAACAGC
    TTGCTGGAGA CAGAGAAACA ATACTCCATG TTGGACTGTG ACCTCAAACA AGCCAAGCAA
    AAGATCAACG AGTTGGAATC ACTTAAAGAC AAACTTAGCG AGGATGTTAA AAACTTAACG
    CTCAAAGCAG AACAAGAGGC ACAGAAGCGC AACCTGACGC ATAACGATCT GAAGATGCAG
    ATACAGCAAG TGAATAGCCT GAAGATGTCT GAGAAGCAAC TGAAGCAAGA GATCAACCAT
    CTGACTGAGA TAAAACTTAA CCTGGAGAAA CAGAACAACG AGTTGCGCAA AGAAAGAGTA
    GATGCGGATG GACAGATGAA GGAGCTTCAA GACCAACTGG AGGCTGAGCA GTATTTCTCA
    ACGCTGTACA AGACGCAGGT GCGGGAGCTG AAAGAGGAGT GTGAAGACAA GAGCAAGATG
    TGCAAAGAAG TGCAGCAGAA GGTCCAAGAG CTTCAGGATG AGAGAGACTC CCTGGCGGCT
    CAACTGGAGA TCACATTGAC CAAGGCCGAC TCCGAGCAGC TGGCCCGTTC CATTGCCGAG
    GAACAGTATT CCGACCTGGA GAAAGAGAAG ATCATGAAGG AACTAGAGAT TAAGGAGATG
    ATGGCACGGC ACAAGCAGGA GCTGGCAGAA AAATATGCTA CAATTGCTTC TTTGGAAGAA
    GCTAACAAAA CACTGACTAT CGATGTAGGC AATTTGGCTA ATGAGAAGGA AGACTTAAAC
    AACAAACTGA AAGAAACCCA AGAACTAATT CAGAATCTGA AAGAGGAAGA AAGCAGCATT
    ATTACCGTCA AGGCACAGTT TGAGAAGCAG CTGTTAACTG AGAGGACTTT GAAGACACAG
    GCTGTAAACA AGTTAGCCGA AATCATGAAC CGCAAGTTAC CCACGAAGCG AGGGCCGGAC
    ACAGATGTAC GAAGAAAGGA AAAGGAGAAC CGCAAGCTGC AGTTGGACCT GAAATCGGAG
    CGTGAGAAAT TCTCTCAGCT TGTCTTCAAA TACCAGAGGG AAGTGAATGA CATGCAGGCG
    CTAATAGCAG ATGAGAACCA AGCCCGAGTC GAACTGCAGA TGGCGCTAGA TAGCAAAGAC
    AGTGACATAG AGCAGCTCCG GTCGCAACTG CAGTCTATGA ACCTCGGCCT TGACAGCACA
    AGTATCGGCA GTGGACACGG TGACACAGAT GCTGATGATG GCTTTCCAGA ATCCAGGCTG
    GAAGGATGGT TGTCGCTTCC TTTGCGCAAC GCCAAAAAGT TTGGTTGGAA TAAGAAGTAC
    GTAGTGGTGA GCAGCAGGAA GATCCTGTTT TATGACAGTG AGCAGGATAA GGAGCTGTCC
    AACCCTTCCA TGGTGCTGGA TATTGATAAG TTGTTCCATG TGCGACCAGT TACTCAGACG
    GATGTGTATC GAGCCGATGC CAAAGAAATT CCACGGATTT TCCAGATTCT ATACGCCAAT
    GAAGGAGAAA GCAAGAAAGA ACAAGAGTTC CAAGTGGACC CCTTAGAGAA ATCCAATTAC
    ATATGTCATA AAGGACATGA GTTTATCCCC ACACTCTACC ATTTCCCCAC CAGCTGTGAT
    GCTTGTATGA AGCCCCTGTG GCACATGTTC AAACCCCCTG CTGCCCTGGA GTGTCGGCGC
    TGCCATATCA AGTGCCACAA GGATCATATG GACAAGAAAG AAGAGATAAT AGCACCTTGC
    AAAGTGAACT ATGATATTTC CACCGCAAAG AATCTACTAT TACTAGCCAA CTCCACAGAA
    GAACAGCAGA AGTGGGTGAG TCGACTGGTG AAAAAGATCC CCAAGAAGCC CCCTGCTTCT
    GACCATCAAG CTCGGTCCTC CCCCCGGCCT CCTGCTAAGG CGTCGCTAAA CCAGTCCATG
    AGGCGGCCAA GCCGGCAGCT TCCTCCAAAT AAACCCAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002942227.2
    CDS976..5097
    Translation

    Target ORF information:

    RefSeq Version XM_002942181.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002942181.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GCAGTCAGCG CACTTGCAAA AAGTCATTAA CAAGCTGGAA
    GAGCAGCTCC ATAATGAGAT GCAAACCAAA GACGAACTGG AGCAAAAGTT CAGAGCTGTA
    AATTTACGTT TGGAGAAGAC AGTGAAGGAG CTGGATGAAG AGGCCAATGC TAGGAAGAGC
    ATAGAATCGA CCACCAGGCA GCTGGAGCGT GAGAAGGCTC TTCTGCAGCA CAAGAGCACA
    GAGTACCAAA GAAAAGCAGA GAATGATGCC GACAGGAAGC GCAGCCTGGA AAATGAAGTT
    AATAGTTTAA AGGATCAGCT TGAAGATTTG AAAAGAAGAA ACCAAAATTC CCAAATATCA
    AATGAGAAGA TGAATCAGCT CCAAAGACAG CTGGATGAAG CCAACGCACA GCTTCGCACA
    GAGTCTGATG CAGCTGCCCG GCTGAGGAAG ACTCAGACCG AGATGTCCAA ACAAATACAG
    CAGCTAGAAA CCAACAACCG TGAGCTTCAG GATAAGACTT GCATGTTGGA GAATGCCAAG
    CTGAAACTAG AAAAGGACTT TATTAACCTG CAGTCGGCTT TGGAGTCAGA ACGGAGGGAC
    CGGACTCAGG GGTCAGAAGT AATAAGTGAC CTTCAAGGAC GAATATCTGT CTTGGAAGAG
    GATCTAAAGA AAGGCAAAGA GCTGCTAGCA AGAGCAGAAG CAGAAAAGCA GCAAATGCAT
    GAGGGGTTAG CCATTTTGGA GAAGGAAAAG AGCAATCTGG AAATCGATAT GACCTACAAA
    CTAAAAGCAC TACAGCAAAG CGTTGAGAAA GAGGAGGCTG AACATAAAGC CACAAAAGCT
    CGGCTGGCTG ACAAGAACAA AATTTACCAG TCCATTGAGG AGACCAAGTC AGAAGCAATG
    AAAGACATGG AGAAGAAGCT TCATGAGGAG CGCCTGGTTA AGCAGAGGCT AGAGAACAGC
    TTGCTGGAGA CAGAGAAACA ATACTCCATG TTGGACTGTG ACCTCAAACA AGCCAAGCAA
    AAGATCAACG AGTTGGAATC ACTTAAAGAC AAACTTAGCG AGGATGTTAA AAACTTAACG
    CTCAAAGCAG AACAAGAGGC ACAGAAGCGC AACCTGACGC ATAACGATCT GAAGATGCAG
    ATACAGCAAG TGAATAGCCT GAAGATGTCT GAGAAGCAAC TGAAGCAAGA GATCAACCAT
    CTGACTGAGA TAAAACTTAA CCTGGAGAAA CAGAACAACG AGTTGCGCAA AGAAAGAGTA
    GATGCGGATG GACAGATGAA GGAGCTTCAA GACCAACTGG AGGCTGAGCA GTATTTCTCA
    ACGCTGTACA AGACGCAGGT GCGGGAGCTG AAAGAGGAGT GTGAAGACAA GAGCAAGATG
    TGCAAAGAAG TGCAGCAGAA GGTCCAAGAG CTTCAGGATG AGAGAGACTC CCTGGCGGCT
    CAACTGGAGA TCACATTGAC CAAGGCCGAC TCCGAGCAGC TGGCCCGTTC CATTGCCGAG
    GAACAGTATT CCGACCTGGA GAAAGAGAAG ATCATGAAGG AACTAGAGAT TAAGGAGATG
    ATGGCACGGC ACAAGCAGGA GCTGGCAGAA AAATATGCTA CAATTGCTTC TTTGGAAGAA
    GCTAACAAAA CACTGACTAT CGATGTAGGC AATTTGGCTA ATGAGAAGGA AGACTTAAAC
    AACAAACTGA AAGAAACCCA AGAACTAATT CAGAATCTGA AAGAGGAAGA AAGCAGCATT
    ATTACCGTCA AGGCACAGTT TGAGAAGCAG CTGTTAACTG AGAGGACTTT GAAGACACAG
    GCTGTAAACA AGTTAGCCGA AATCATGAAC CGCAAGTTAC CCACGAAGCG AGGGCCGGAC
    ACAGATGTAC GAAGAAAGGA AAAGGAGAAC CGCAAGCTGC AGTTGGACCT GAAATCGGAG
    CGTGAGAAAT TCTCTCAGCT TGTCTTCAAA TACCAGAGGG AAGTGAATGA CATGCAGGCG
    CTAATAGCAG ATGAGAACCA AGCCCGAGTC GAACTGCAGA TGGCGCTAGA TAGCAAAGAC
    AGTGACATAG AGCAGCTCCG GTCGCAACTG CAGTCTATGA ACCTCGGCCT TGACAGCACA
    AGTATCGGCA GTGGACACGG TGACACAGAT GCTGATGATG GCTTTCCAGA ATCCAGGCTG
    GAAGGATGGT TGTCGCTTCC TTTGCGCAAC GCCAAAAAGT TTGGTTGGAA TAAGAAGTAC
    GTAGTGGTGA GCAGCAGGAA GATCCTGTTT TATGACAGTG AGCAGGATAA GGAGCTGTCC
    AACCCTTCCA TGGTGCTGGA TATTGATAAG TTGTTCCATG TGCGACCAGT TACTCAGACG
    GATGTGTATC GAGCCGATGC CAAAGAAATT CCACGGATTT TCCAGATTCT ATACGCCAAT
    GAAGGAGAAA GCAAGAAAGA ACAAGAGTTC CAAGTGGACC CCTTAGAGAA ATCCAATTAC
    ATATGTCATA AAGGACATGA GTTTATCCCC ACACTCTACC ATTTCCCCAC CAGCTGTGAT
    GCTTGTATGA AGCCCCTGTG GCACATGTTC AAACCCCCTG CTGCCCTGGA GTGTCGGCGC
    TGCCATATCA AGTGCCACAA GGATCATATG GACAAGAAAG AAGAGATAAT AGCACCTTGC
    AAAGTGAACT ATGATATTTC CACCGCAAAG AATCTACTAT TACTAGCCAA CTCCACAGAA
    GAACAGCAGA AGTGGGTGAG TCGACTGGTG AAAAAGATCC CCAAGAAGCC CCCTGCTTCT
    GACCATCAAG CTCGGTCCTC CCCCCGGCCT CCTGCTAAGG CGTCGCTAAA CCAGTCCATG
    AGGCGGCCAA GCCGGCAGCT TCCTCCAAAT AAACCCAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44819
    Clone ID Related Accession (Same CDS sequence) XM_018094480.1
    Accession Version XM_018094480.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho-associated protein kinase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GCAGTCAGCG CACTTGCAAA AAGTCATTAA CAAGCTGGAA
    GAGCAGCTCC ATAATGAGAT GCAAACCAAA GACGAACTGG AGCAAAAGTT CAGAGCTGTA
    AATTTACGTT TGGAGAAGAC AGTGAAGGAG CTGGATGAAG AGGCCAATGC TAGGAAGAGC
    ATAGAATCGA CCACCAGGCA GCTGGAGCGT GAGAAGGCTC TTCTGCAGCA CAAGAGCACA
    GAGTACCAAA GAAAAGCAGA GAATGATGCC GACAGGAAGC GCAGCCTGGA AAATGAAGTT
    AATAGTTTAA AGGATCAGCT TGAAGATTTG AAAAGAAGAA ACCAAAATTC CCAAATATCA
    AATGAGAAGA TGAATCAGCT CCAAAGACAG CTGGATGAAG CCAACGCACA GCTTCGCACA
    GAGTCTGATG CAGCTGCCCG GCTGAGGAAG ACTCAGACCG AGATGTCCAA ACAAATACAG
    CAGCTAGAAA CCAACAACCG TGAGCTTCAG GATAAGACTT GCATGTTGGA GAATGCCAAG
    CTGAAACTAG AAAAGGACTT TATTAACCTG CAGTCGGCTT TGGAGTCAGA ACGGAGGGAC
    CGGACTCAGG GGTCAGAAGT AATAAGTGAC CTTCAAGGAC GAATATCTGT CTTGGAAGAG
    GATCTAAAGA AAGGCAAAGA GCTGCTAGCA AGAGCAGAAG CAGAAAAGCA GCAAATGCAT
    GAGGGGTTAG CCATTTTGGA GAAGGAAAAG AGCAATCTGG AAATCGATAT GACCTACAAA
    CTAAAAGCAC TACAGCAAAG CGTTGAGAAA GAGGAGGCTG AACATAAAGC CACAAAAGCT
    CGGCTGGCTG ACAAGAACAA AATTTACCAG TCCATTGAGG AGACCAAGTC AGAAGCAATG
    AAAGACATGG AGAAGAAGCT TCATGAGGAG CGCCTGGTTA AGCAGAGGCT AGAGAACAGC
    TTGCTGGAGA CAGAGAAACA ATACTCCATG TTGGACTGTG ACCTCAAACA AGCCAAGCAA
    AAGATCAACG AGTTGGAATC ACTTAAAGAC AAACTTAGCG AGGATGTTAA AAACTTAACG
    CTCAAAGCAG AACAAGAGGC ACAGAAGCGC AACCTGACGC ATAACGATCT GAAGATGCAG
    ATACAGCAAG TGAATAGCCT GAAGATGTCT GAGAAGCAAC TGAAGCAAGA GATCAACCAT
    CTGACTGAGA TAAAACTTAA CCTGGAGAAA CAGAACAACG AGTTGCGCAA AGAAAGAGTA
    GATGCGGATG GACAGATGAA GGAGCTTCAA GACCAACTGG AGGCTGAGCA GTATTTCTCA
    ACGCTGTACA AGACGCAGGT GCGGGAGCTG AAAGAGGAGT GTGAAGACAA GAGCAAGATG
    TGCAAAGAAG TGCAGCAGAA GGTCCAAGAG CTTCAGGATG AGAGAGACTC CCTGGCGGCT
    CAACTGGAGA TCACATTGAC CAAGGCCGAC TCCGAGCAGC TGGCCCGTTC CATTGCCGAG
    GAACAGTATT CCGACCTGGA GAAAGAGAAG ATCATGAAGG AACTAGAGAT TAAGGAGATG
    ATGGCACGGC ACAAGCAGGA GCTGGCAGAA AAATATGCTA CAATTGCTTC TTTGGAAGAA
    GCTAACAAAA CACTGACTAT CGATGTAGGC AATTTGGCTA ATGAGAAGGA AGACTTAAAC
    AACAAACTGA AAGAAACCCA AGAACTAATT CAGAATCTGA AAGAGGAAGA AAGCAGCATT
    ATTACCGTCA AGGCACAGTT TGAGAAGCAG CTGTTAACTG AGAGGACTTT GAAGACACAG
    GCTGTAAACA AGTTAGCCGA AATCATGAAC CGCAAGTTAC CCACGAAGCG AGGGCCGGAC
    ACAGATGTAC GAAGAAAGGA AAAGGAGAAC CGCAAGCTGC AGTTGGACCT GAAATCGGAG
    CGTGAGAAAT TCTCTCAGCT TGTCTTCAAA TACCAGAGGG AAGTGAATGA CATGCAGGCG
    CTAATAGCAG ATGAGAACCA AGCCCGAGTC GAACTGCAGA TGGCGCTAGA TAGCAAAGAC
    AGTGACATAG AGCAGCTCCG GTCGCAACTG CAGTCTATGA ACCTCGGCCT TGACAGCACA
    AGTATCGGCA GTGGACACGG TGACACAGAT GCTGATGATG GCTTTCCAGT TCACATCACC
    CAGTCCCGTA CTACGGAGTC CATGTCATTT ACCTATCAGC GTTCTTCTAC CTCTGTCACC
    ATTGGCACTA AGCCCTTGCG TTCTCGCTTG CTTCCCGACT CTGACTCTGA CTCAGATGAG
    GAACCCTTCC CTTGTGCACC AGTCGTTACT GAACCTGATA GTGATGATAG TGAAGGTGAC
    GTCTCTGTGC ACCACTGTGT TCTGCACTGT TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949969.1
    CDS977..4612
    Translation

    Target ORF information:

    RefSeq Version XM_018094480.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094480.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GCAGTCAGCG CACTTGCAAA AAGTCATTAA CAAGCTGGAA
    GAGCAGCTCC ATAATGAGAT GCAAACCAAA GACGAACTGG AGCAAAAGTT CAGAGCTGTA
    AATTTACGTT TGGAGAAGAC AGTGAAGGAG CTGGATGAAG AGGCCAATGC TAGGAAGAGC
    ATAGAATCGA CCACCAGGCA GCTGGAGCGT GAGAAGGCTC TTCTGCAGCA CAAGAGCACA
    GAGTACCAAA GAAAAGCAGA GAATGATGCC GACAGGAAGC GCAGCCTGGA AAATGAAGTT
    AATAGTTTAA AGGATCAGCT TGAAGATTTG AAAAGAAGAA ACCAAAATTC CCAAATATCA
    AATGAGAAGA TGAATCAGCT CCAAAGACAG CTGGATGAAG CCAACGCACA GCTTCGCACA
    GAGTCTGATG CAGCTGCCCG GCTGAGGAAG ACTCAGACCG AGATGTCCAA ACAAATACAG
    CAGCTAGAAA CCAACAACCG TGAGCTTCAG GATAAGACTT GCATGTTGGA GAATGCCAAG
    CTGAAACTAG AAAAGGACTT TATTAACCTG CAGTCGGCTT TGGAGTCAGA ACGGAGGGAC
    CGGACTCAGG GGTCAGAAGT AATAAGTGAC CTTCAAGGAC GAATATCTGT CTTGGAAGAG
    GATCTAAAGA AAGGCAAAGA GCTGCTAGCA AGAGCAGAAG CAGAAAAGCA GCAAATGCAT
    GAGGGGTTAG CCATTTTGGA GAAGGAAAAG AGCAATCTGG AAATCGATAT GACCTACAAA
    CTAAAAGCAC TACAGCAAAG CGTTGAGAAA GAGGAGGCTG AACATAAAGC CACAAAAGCT
    CGGCTGGCTG ACAAGAACAA AATTTACCAG TCCATTGAGG AGACCAAGTC AGAAGCAATG
    AAAGACATGG AGAAGAAGCT TCATGAGGAG CGCCTGGTTA AGCAGAGGCT AGAGAACAGC
    TTGCTGGAGA CAGAGAAACA ATACTCCATG TTGGACTGTG ACCTCAAACA AGCCAAGCAA
    AAGATCAACG AGTTGGAATC ACTTAAAGAC AAACTTAGCG AGGATGTTAA AAACTTAACG
    CTCAAAGCAG AACAAGAGGC ACAGAAGCGC AACCTGACGC ATAACGATCT GAAGATGCAG
    ATACAGCAAG TGAATAGCCT GAAGATGTCT GAGAAGCAAC TGAAGCAAGA GATCAACCAT
    CTGACTGAGA TAAAACTTAA CCTGGAGAAA CAGAACAACG AGTTGCGCAA AGAAAGAGTA
    GATGCGGATG GACAGATGAA GGAGCTTCAA GACCAACTGG AGGCTGAGCA GTATTTCTCA
    ACGCTGTACA AGACGCAGGT GCGGGAGCTG AAAGAGGAGT GTGAAGACAA GAGCAAGATG
    TGCAAAGAAG TGCAGCAGAA GGTCCAAGAG CTTCAGGATG AGAGAGACTC CCTGGCGGCT
    CAACTGGAGA TCACATTGAC CAAGGCCGAC TCCGAGCAGC TGGCCCGTTC CATTGCCGAG
    GAACAGTATT CCGACCTGGA GAAAGAGAAG ATCATGAAGG AACTAGAGAT TAAGGAGATG
    ATGGCACGGC ACAAGCAGGA GCTGGCAGAA AAATATGCTA CAATTGCTTC TTTGGAAGAA
    GCTAACAAAA CACTGACTAT CGATGTAGGC AATTTGGCTA ATGAGAAGGA AGACTTAAAC
    AACAAACTGA AAGAAACCCA AGAACTAATT CAGAATCTGA AAGAGGAAGA AAGCAGCATT
    ATTACCGTCA AGGCACAGTT TGAGAAGCAG CTGTTAACTG AGAGGACTTT GAAGACACAG
    GCTGTAAACA AGTTAGCCGA AATCATGAAC CGCAAGTTAC CCACGAAGCG AGGGCCGGAC
    ACAGATGTAC GAAGAAAGGA AAAGGAGAAC CGCAAGCTGC AGTTGGACCT GAAATCGGAG
    CGTGAGAAAT TCTCTCAGCT TGTCTTCAAA TACCAGAGGG AAGTGAATGA CATGCAGGCG
    CTAATAGCAG ATGAGAACCA AGCCCGAGTC GAACTGCAGA TGGCGCTAGA TAGCAAAGAC
    AGTGACATAG AGCAGCTCCG GTCGCAACTG CAGTCTATGA ACCTCGGCCT TGACAGCACA
    AGTATCGGCA GTGGACACGG TGACACAGAT GCTGATGATG GCTTTCCAGT TCACATCACC
    CAGTCCCGTA CTACGGAGTC CATGTCATTT ACCTATCAGC GTTCTTCTAC CTCTGTCACC
    ATTGGCACTA AGCCCTTGCG TTCTCGCTTG CTTCCCGACT CTGACTCTGA CTCAGATGAG
    GAACCCTTCC CTTGTGCACC AGTCGTTACT GAACCTGATA GTGATGATAG TGAAGGTGAC
    GTCTCTGTGC ACCACTGTGT TCTGCACTGT TTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10314
    Clone ID Related Accession (Same CDS sequence) XM_004914468.2 , XM_004914468.3
    Accession Version XM_004914468.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho-associated protein kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004914468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GCAGTCAGCG CACTTGCAAA AAGTCATTAA CAAGCTGGAA
    GAGCAGCTCC ATAATGAGAT GCAAACCAAA GACGAACTGG AGCAAAAGTT CAGAGCTGTA
    AATTTACGTT TGGAGAAGAC AGTGAAGGAG CTGGATGAAG AGGCCAATGC TAGGAAGAGC
    ATAGAATCGA CCACCAGGCA GCTGGAGCGT GAGAAGGCTC TTCTGCAGCA CAAGAGCACA
    GAGTACCAAA GAAAAGCAGA GAATGATGCC GACAGGAAGC GCAGCCTGGA AAATGAAGTT
    AATAGTTTAA AGGATCAGCT TGAAGATTTG AAAAGAAGAA ACCAAAATTC CCAAATATCA
    AATGAGAAGA TGAATCAGCT CCAAAGACAG CTGGATGAAG CCAACGCACA GCTTCGCACA
    GAGTCTGATG CAGCTGCCCG GCTGAGGAAG ACTCAGACCG AGATGTCCAA ACAAATACAG
    CAGCTAGAAA CCAACAACCG TGAGCTTCAG GATAAGACTT GCATGTTGGA GAATGCCAAG
    CTGAAACTAG AAAAGGACTT TATTAACCTG CAGTCGGCTT TGGAGTCAGA ACGGAGGGAC
    CGGACTCAGG GGTCAGAAGT AATAAGTGAC CTTCAAGGAC GAATATCTGT CTTGGAAGAG
    GATCTAAAGA AAGGCAAAGA GCTGCTAGCA AGAGCAGAAG CAGAAAAGCA GCAAATGCAT
    GAGGGGTTAG CCATTTTGGA GAAGGAAAAG AGCAATCTGG AAATCGATAT GACCTACAAA
    CTAAAAGCAC TACAGCAAAG CGTTGAGAAA GAGGAGGCTG AACATAAAGC CACAAAAGCT
    CGGCTGGCTG ACAAGAACAA AATTTACCAG TCCATTGAGG AGACCAAGTC AGAAGCAATG
    AAAGACATGG AGAAGAAGCT TCATGAGGAG CGCCTGGTTA AGCAGAGGCT AGAGAACAGC
    TTGCTGGAGA CAGAGAAACA ATACTCCATG TTGGACTGTG ACCTCAAACA AGCCAAGCAA
    AAGATCAACG AGTTGGAATC ACTTAAAGAC AAACTTAGCG AGGATGTTAA AAACTTAACG
    CTCAAAGCAG AACAAGAGGC ACAGAAGCGC AACCTGACGC ATAACGATCT GAAGATGCAG
    ATACAGCAAG TGAATAGCCT GAAGATGTCT GAGAAGCAAC TGAAGCAAGA GATCAACCAT
    CTGACTGAGA TAAAACTTAA CCTGGAGAAA CAGAACAACG AGTTGCGCAA AGAAAGAGTA
    GATGCGGATG GACAGATGAA GGAGCTTCAA GACCAACTGG AGGCTGAGCA GTATTTCTCA
    ACGCTGTACA AGACGCAGGT GCGGGAGCTG AAAGAGGAGT GTGAAGACAA GAGCAAGATG
    TGCAAAGAAG TGCAGCAGAA GGTCCAAGAG CTTCAGGATG AGAGAGACTC CCTGGCGGCT
    CAACTGGAGA TCACATTGAC CAAGGCCGAC TCCGAGCAGC TGGCCCGTTC CATTGCCGAG
    GAACAGTATT CCGACCTGGA GAAAGAGAAG ATCATGAAGG AACTAGAGAT TAAGGAGATG
    ATGGCACGGC ACAAGCAGGA GCTGGCAGAA AAATATGCTA CAATTGCTTC TTTGGAAGAA
    GCTAACAAAA CACTGACTAT CGATGTAGGC AATTTGGCTA ATGAGAAGGA AGACTTAAAC
    AACAAACTGA AAGAAACCCA AGAACTAATT CAGAATCTGA AAGAGGAAGA AAGCAGCATT
    ATTACCGTCA AGGCACAGTT TGAGAAGCAG CTGTTAACTG AGAGGACTTT GAAGACACAG
    GCTGTAAACA AGTTAGCCGA AATCATGAAC CGCAAGTTAC CCACGAAGCG AGGGCCGGAC
    ACAGATGTAC GAAGAAAGGA AAAGGAGAAC CGCAAGCTGC AGTTGGACCT GAAATCGGAG
    CGTGAGAAAT TCTCTCAGCT TGTCTTCAAA TACCAGAGGG AAGTGAATGA CATGCAGGCG
    CTAATAGCAG ATGAGAACCA AGCCCGAGTC GAACTGCAGA TGGCGCTAGA TAGCAAAGAC
    AGTGACATAG AGCAGCTCCG GTCGCAACTG CAGTCTATGA ACCTCGGCCT TGACAGCACA
    AGTATCGGCA GTGGACACGG TGACACAGAT GCTGATGATG GCTTTCCAGA ATCCAGGCTG
    GAAGGATGGT TGTCGCTTCC TTTGCGCAAC GCCAAAAAGT TTGGTTGGAA TAAGAAGTAC
    GTAGTGGTGA GCAGCAGGAA GATCCTGTTT TATGACAGTG AGCAGGATAA GGAGCTGTCC
    AACCCTTCCA TGGTGCTGGA TATTGATAAG TTGTTCCATG TGCGACCAGT TACTCAGACG
    GATGTGTATC GAGCCGATGC CAAAGAAATT CCACGGATTT TCCAGATTCT ATACGCCAAT
    GAAGGAGAAA GCAAGAAAGA ACAAGAGTTC CAAGTGGACC CCTTAGAGAA ATCCAATTAC
    ATATGTCATA AAGGACATGA GTTTATCCCC ACACTCTACC ATTTCCCCAC CAGCTGTGAT
    GCTTGTATGA AGCCCCTGTG GCACATGTTC AAACCCCCTG CTGCCCTGGA GTGTCGGCGC
    TGCCATATCA AGTGCCACAA GGATCATATG GACAAGAAAG AAGAGATAAT AGCACCTTGC
    AAAGTGAACT ATGATATTTC CACCGCAAAG AATCTACTAT TACTAGCCAA CTCCACAGAA
    GAACAGCAGA AGTGGGTGAG TCGACTGGTG AAAAAGATCC CCAAGAAGCC CCCTGCTTCT
    GACCATCAAG CTCGGTCCTC CCCCCGGCCT CCTGCTAAGG CGTCGCTAAA CCAGTCCATG
    AGGCGGCCAA GCCGGCAGCT TCCTCCAAAT AAACCCAGAT TACTTGATCT GGCATTTAAA
    GACTGGGACT GGTCTCTTGA TGATGTAGAG GATGATGATG AGGATGTTTT TGATTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914525.1
    CDS977..5176
    Translation

    Target ORF information:

    RefSeq Version XM_004914468.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914468.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GCAGTCAGCG CACTTGCAAA AAGTCATTAA CAAGCTGGAA
    GAGCAGCTCC ATAATGAGAT GCAAACCAAA GACGAACTGG AGCAAAAGTT CAGAGCTGTA
    AATTTACGTT TGGAGAAGAC AGTGAAGGAG CTGGATGAAG AGGCCAATGC TAGGAAGAGC
    ATAGAATCGA CCACCAGGCA GCTGGAGCGT GAGAAGGCTC TTCTGCAGCA CAAGAGCACA
    GAGTACCAAA GAAAAGCAGA GAATGATGCC GACAGGAAGC GCAGCCTGGA AAATGAAGTT
    AATAGTTTAA AGGATCAGCT TGAAGATTTG AAAAGAAGAA ACCAAAATTC CCAAATATCA
    AATGAGAAGA TGAATCAGCT CCAAAGACAG CTGGATGAAG CCAACGCACA GCTTCGCACA
    GAGTCTGATG CAGCTGCCCG GCTGAGGAAG ACTCAGACCG AGATGTCCAA ACAAATACAG
    CAGCTAGAAA CCAACAACCG TGAGCTTCAG GATAAGACTT GCATGTTGGA GAATGCCAAG
    CTGAAACTAG AAAAGGACTT TATTAACCTG CAGTCGGCTT TGGAGTCAGA ACGGAGGGAC
    CGGACTCAGG GGTCAGAAGT AATAAGTGAC CTTCAAGGAC GAATATCTGT CTTGGAAGAG
    GATCTAAAGA AAGGCAAAGA GCTGCTAGCA AGAGCAGAAG CAGAAAAGCA GCAAATGCAT
    GAGGGGTTAG CCATTTTGGA GAAGGAAAAG AGCAATCTGG AAATCGATAT GACCTACAAA
    CTAAAAGCAC TACAGCAAAG CGTTGAGAAA GAGGAGGCTG AACATAAAGC CACAAAAGCT
    CGGCTGGCTG ACAAGAACAA AATTTACCAG TCCATTGAGG AGACCAAGTC AGAAGCAATG
    AAAGACATGG AGAAGAAGCT TCATGAGGAG CGCCTGGTTA AGCAGAGGCT AGAGAACAGC
    TTGCTGGAGA CAGAGAAACA ATACTCCATG TTGGACTGTG ACCTCAAACA AGCCAAGCAA
    AAGATCAACG AGTTGGAATC ACTTAAAGAC AAACTTAGCG AGGATGTTAA AAACTTAACG
    CTCAAAGCAG AACAAGAGGC ACAGAAGCGC AACCTGACGC ATAACGATCT GAAGATGCAG
    ATACAGCAAG TGAATAGCCT GAAGATGTCT GAGAAGCAAC TGAAGCAAGA GATCAACCAT
    CTGACTGAGA TAAAACTTAA CCTGGAGAAA CAGAACAACG AGTTGCGCAA AGAAAGAGTA
    GATGCGGATG GACAGATGAA GGAGCTTCAA GACCAACTGG AGGCTGAGCA GTATTTCTCA
    ACGCTGTACA AGACGCAGGT GCGGGAGCTG AAAGAGGAGT GTGAAGACAA GAGCAAGATG
    TGCAAAGAAG TGCAGCAGAA GGTCCAAGAG CTTCAGGATG AGAGAGACTC CCTGGCGGCT
    CAACTGGAGA TCACATTGAC CAAGGCCGAC TCCGAGCAGC TGGCCCGTTC CATTGCCGAG
    GAACAGTATT CCGACCTGGA GAAAGAGAAG ATCATGAAGG AACTAGAGAT TAAGGAGATG
    ATGGCACGGC ACAAGCAGGA GCTGGCAGAA AAATATGCTA CAATTGCTTC TTTGGAAGAA
    GCTAACAAAA CACTGACTAT CGATGTAGGC AATTTGGCTA ATGAGAAGGA AGACTTAAAC
    AACAAACTGA AAGAAACCCA AGAACTAATT CAGAATCTGA AAGAGGAAGA AAGCAGCATT
    ATTACCGTCA AGGCACAGTT TGAGAAGCAG CTGTTAACTG AGAGGACTTT GAAGACACAG
    GCTGTAAACA AGTTAGCCGA AATCATGAAC CGCAAGTTAC CCACGAAGCG AGGGCCGGAC
    ACAGATGTAC GAAGAAAGGA AAAGGAGAAC CGCAAGCTGC AGTTGGACCT GAAATCGGAG
    CGTGAGAAAT TCTCTCAGCT TGTCTTCAAA TACCAGAGGG AAGTGAATGA CATGCAGGCG
    CTAATAGCAG ATGAGAACCA AGCCCGAGTC GAACTGCAGA TGGCGCTAGA TAGCAAAGAC
    AGTGACATAG AGCAGCTCCG GTCGCAACTG CAGTCTATGA ACCTCGGCCT TGACAGCACA
    AGTATCGGCA GTGGACACGG TGACACAGAT GCTGATGATG GCTTTCCAGA ATCCAGGCTG
    GAAGGATGGT TGTCGCTTCC TTTGCGCAAC GCCAAAAAGT TTGGTTGGAA TAAGAAGTAC
    GTAGTGGTGA GCAGCAGGAA GATCCTGTTT TATGACAGTG AGCAGGATAA GGAGCTGTCC
    AACCCTTCCA TGGTGCTGGA TATTGATAAG TTGTTCCATG TGCGACCAGT TACTCAGACG
    GATGTGTATC GAGCCGATGC CAAAGAAATT CCACGGATTT TCCAGATTCT ATACGCCAAT
    GAAGGAGAAA GCAAGAAAGA ACAAGAGTTC CAAGTGGACC CCTTAGAGAA ATCCAATTAC
    ATATGTCATA AAGGACATGA GTTTATCCCC ACACTCTACC ATTTCCCCAC CAGCTGTGAT
    GCTTGTATGA AGCCCCTGTG GCACATGTTC AAACCCCCTG CTGCCCTGGA GTGTCGGCGC
    TGCCATATCA AGTGCCACAA GGATCATATG GACAAGAAAG AAGAGATAAT AGCACCTTGC
    AAAGTGAACT ATGATATTTC CACCGCAAAG AATCTACTAT TACTAGCCAA CTCCACAGAA
    GAACAGCAGA AGTGGGTGAG TCGACTGGTG AAAAAGATCC CCAAGAAGCC CCCTGCTTCT
    GACCATCAAG CTCGGTCCTC CCCCCGGCCT CCTGCTAAGG CGTCGCTAAA CCAGTCCATG
    AGGCGGCCAA GCCGGCAGCT TCCTCCAAAT AAACCCAGAT TACTTGATCT GGCATTTAAA
    GACTGGGACT GGTCTCTTGA TGATGTAGAG GATGATGATG AGGATGTTTT TGATTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10315
    Clone ID Related Accession (Same CDS sequence) XM_004914469.2 , XM_004914469.3
    Accession Version XM_004914469.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho-associated protein kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004914469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GTCAGCGCAC TTGCAAAAAG TCATTAACAA GCTGGAAGAG
    CAGCTCCATA ATGAGATGCA AACCAAAGAC GAACTGGAGC AAAAGTTCAG AGCTGTAAAT
    TTACGTTTGG AGAAGACAGT GAAGGAGCTG GATGAAGAGG CCAATGCTAG GAAGAGCATA
    GAATCGACCA CCAGGCAGCT GGAGCGTGAG AAGGCTCTTC TGCAGCACAA GAGCACAGAG
    TACCAAAGAA AAGCAGAGAA TGATGCCGAC AGGAAGCGCA GCCTGGAAAA TGAAGTTAAT
    AGTTTAAAGG ATCAGCTTGA AGATTTGAAA AGAAGAAACC AAAATTCCCA AATATCAAAT
    GAGAAGATGA ATCAGCTCCA AAGACAGCTG GATGAAGCCA ACGCACAGCT TCGCACAGAG
    TCTGATGCAG CTGCCCGGCT GAGGAAGACT CAGACCGAGA TGTCCAAACA AATACAGCAG
    CTAGAAACCA ACAACCGTGA GCTTCAGGAT AAGACTTGCA TGTTGGAGAA TGCCAAGCTG
    AAACTAGAAA AGGACTTTAT TAACCTGCAG TCGGCTTTGG AGTCAGAACG GAGGGACCGG
    ACTCAGGGGT CAGAAGTAAT AAGTGACCTT CAAGGACGAA TATCTGTCTT GGAAGAGGAT
    CTAAAGAAAG GCAAAGAGCT GCTAGCAAGA GCAGAAGCAG AAAAGCAGCA AATGCATGAG
    GGGTTAGCCA TTTTGGAGAA GGAAAAGAGC AATCTGGAAA TCGATATGAC CTACAAACTA
    AAAGCACTAC AGCAAAGCGT TGAGAAAGAG GAGGCTGAAC ATAAAGCCAC AAAAGCTCGG
    CTGGCTGACA AGAACAAAAT TTACCAGTCC ATTGAGGAGA CCAAGTCAGA AGCAATGAAA
    GACATGGAGA AGAAGCTTCA TGAGGAGCGC CTGGTTAAGC AGAGGCTAGA GAACAGCTTG
    CTGGAGACAG AGAAACAATA CTCCATGTTG GACTGTGACC TCAAACAAGC CAAGCAAAAG
    ATCAACGAGT TGGAATCACT TAAAGACAAA CTTAGCGAGG ATGTTAAAAA CTTAACGCTC
    AAAGCAGAAC AAGAGGCACA GAAGCGCAAC CTGACGCATA ACGATCTGAA GATGCAGATA
    CAGCAAGTGA ATAGCCTGAA GATGTCTGAG AAGCAACTGA AGCAAGAGAT CAACCATCTG
    ACTGAGATAA AACTTAACCT GGAGAAACAG AACAACGAGT TGCGCAAAGA AAGAGTAGAT
    GCGGATGGAC AGATGAAGGA GCTTCAAGAC CAACTGGAGG CTGAGCAGTA TTTCTCAACG
    CTGTACAAGA CGCAGGTGCG GGAGCTGAAA GAGGAGTGTG AAGACAAGAG CAAGATGTGC
    AAAGAAGTGC AGCAGAAGGT CCAAGAGCTT CAGGATGAGA GAGACTCCCT GGCGGCTCAA
    CTGGAGATCA CATTGACCAA GGCCGACTCC GAGCAGCTGG CCCGTTCCAT TGCCGAGGAA
    CAGTATTCCG ACCTGGAGAA AGAGAAGATC ATGAAGGAAC TAGAGATTAA GGAGATGATG
    GCACGGCACA AGCAGGAGCT GGCAGAAAAA TATGCTACAA TTGCTTCTTT GGAAGAAGCT
    AACAAAACAC TGACTATCGA TGTAGGCAAT TTGGCTAATG AGAAGGAAGA CTTAAACAAC
    AAACTGAAAG AAACCCAAGA ACTAATTCAG AATCTGAAAG AGGAAGAAAG CAGCATTATT
    ACCGTCAAGG CACAGTTTGA GAAGCAGCTG TTAACTGAGA GGACTTTGAA GACACAGGCT
    GTAAACAAGT TAGCCGAAAT CATGAACCGC AAGTTACCCA CGAAGCGAGG GCCGGACACA
    GATGTACGAA GAAAGGAAAA GGAGAACCGC AAGCTGCAGT TGGACCTGAA ATCGGAGCGT
    GAGAAATTCT CTCAGCTTGT CTTCAAATAC CAGAGGGAAG TGAATGACAT GCAGGCGCTA
    ATAGCAGATG AGAACCAAGC CCGAGTCGAA CTGCAGATGG CGCTAGATAG CAAAGACAGT
    GACATAGAGC AGCTCCGGTC GCAACTGCAG TCTATGAACC TCGGCCTTGA CAGCACAAGT
    ATCGGCAGTG GACACGGTGA CACAGATGCT GATGATGGCT TTCCAGAATC CAGGCTGGAA
    GGATGGTTGT CGCTTCCTTT GCGCAACGCC AAAAAGTTTG GTTGGAATAA GAAGTACGTA
    GTGGTGAGCA GCAGGAAGAT CCTGTTTTAT GACAGTGAGC AGGATAAGGA GCTGTCCAAC
    CCTTCCATGG TGCTGGATAT TGATAAGTTG TTCCATGTGC GACCAGTTAC TCAGACGGAT
    GTGTATCGAG CCGATGCCAA AGAAATTCCA CGGATTTTCC AGATTCTATA CGCCAATGAA
    GGAGAAAGCA AGAAAGAACA AGAGTTCCAA GTGGACCCCT TAGAGAAATC CAATTACATA
    TGTCATAAAG GACATGAGTT TATCCCCACA CTCTACCATT TCCCCACCAG CTGTGATGCT
    TGTATGAAGC CCCTGTGGCA CATGTTCAAA CCCCCTGCTG CCCTGGAGTG TCGGCGCTGC
    CATATCAAGT GCCACAAGGA TCATATGGAC AAGAAAGAAG AGATAATAGC ACCTTGCAAA
    GTGAACTATG ATATTTCCAC CGCAAAGAAT CTACTATTAC TAGCCAACTC CACAGAAGAA
    CAGCAGAAGT GGGTGAGTCG ACTGGTGAAA AAGATCCCCA AGAAGCCCCC TGCTTCTGAC
    CATCAAGCTC GGTCCTCCCC CCGGCCTCCT GCTAAGGCGT CGCTAAACCA GTCCATGAGG
    CGGCCAAGCC GGCAGCTTCC TCCAAATAAA CCCAGATTAC TTGATCTGGC ATTTAAAGAC
    TGGGACTGGT CTCTTGATGA TGTAGAGGAT GATGATGAGG ATGTTTTTGA TTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004914526.1
    CDS977..5173
    Translation

    Target ORF information:

    RefSeq Version XM_004914469.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004914469.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GTCAGCGCAC TTGCAAAAAG TCATTAACAA GCTGGAAGAG
    CAGCTCCATA ATGAGATGCA AACCAAAGAC GAACTGGAGC AAAAGTTCAG AGCTGTAAAT
    TTACGTTTGG AGAAGACAGT GAAGGAGCTG GATGAAGAGG CCAATGCTAG GAAGAGCATA
    GAATCGACCA CCAGGCAGCT GGAGCGTGAG AAGGCTCTTC TGCAGCACAA GAGCACAGAG
    TACCAAAGAA AAGCAGAGAA TGATGCCGAC AGGAAGCGCA GCCTGGAAAA TGAAGTTAAT
    AGTTTAAAGG ATCAGCTTGA AGATTTGAAA AGAAGAAACC AAAATTCCCA AATATCAAAT
    GAGAAGATGA ATCAGCTCCA AAGACAGCTG GATGAAGCCA ACGCACAGCT TCGCACAGAG
    TCTGATGCAG CTGCCCGGCT GAGGAAGACT CAGACCGAGA TGTCCAAACA AATACAGCAG
    CTAGAAACCA ACAACCGTGA GCTTCAGGAT AAGACTTGCA TGTTGGAGAA TGCCAAGCTG
    AAACTAGAAA AGGACTTTAT TAACCTGCAG TCGGCTTTGG AGTCAGAACG GAGGGACCGG
    ACTCAGGGGT CAGAAGTAAT AAGTGACCTT CAAGGACGAA TATCTGTCTT GGAAGAGGAT
    CTAAAGAAAG GCAAAGAGCT GCTAGCAAGA GCAGAAGCAG AAAAGCAGCA AATGCATGAG
    GGGTTAGCCA TTTTGGAGAA GGAAAAGAGC AATCTGGAAA TCGATATGAC CTACAAACTA
    AAAGCACTAC AGCAAAGCGT TGAGAAAGAG GAGGCTGAAC ATAAAGCCAC AAAAGCTCGG
    CTGGCTGACA AGAACAAAAT TTACCAGTCC ATTGAGGAGA CCAAGTCAGA AGCAATGAAA
    GACATGGAGA AGAAGCTTCA TGAGGAGCGC CTGGTTAAGC AGAGGCTAGA GAACAGCTTG
    CTGGAGACAG AGAAACAATA CTCCATGTTG GACTGTGACC TCAAACAAGC CAAGCAAAAG
    ATCAACGAGT TGGAATCACT TAAAGACAAA CTTAGCGAGG ATGTTAAAAA CTTAACGCTC
    AAAGCAGAAC AAGAGGCACA GAAGCGCAAC CTGACGCATA ACGATCTGAA GATGCAGATA
    CAGCAAGTGA ATAGCCTGAA GATGTCTGAG AAGCAACTGA AGCAAGAGAT CAACCATCTG
    ACTGAGATAA AACTTAACCT GGAGAAACAG AACAACGAGT TGCGCAAAGA AAGAGTAGAT
    GCGGATGGAC AGATGAAGGA GCTTCAAGAC CAACTGGAGG CTGAGCAGTA TTTCTCAACG
    CTGTACAAGA CGCAGGTGCG GGAGCTGAAA GAGGAGTGTG AAGACAAGAG CAAGATGTGC
    AAAGAAGTGC AGCAGAAGGT CCAAGAGCTT CAGGATGAGA GAGACTCCCT GGCGGCTCAA
    CTGGAGATCA CATTGACCAA GGCCGACTCC GAGCAGCTGG CCCGTTCCAT TGCCGAGGAA
    CAGTATTCCG ACCTGGAGAA AGAGAAGATC ATGAAGGAAC TAGAGATTAA GGAGATGATG
    GCACGGCACA AGCAGGAGCT GGCAGAAAAA TATGCTACAA TTGCTTCTTT GGAAGAAGCT
    AACAAAACAC TGACTATCGA TGTAGGCAAT TTGGCTAATG AGAAGGAAGA CTTAAACAAC
    AAACTGAAAG AAACCCAAGA ACTAATTCAG AATCTGAAAG AGGAAGAAAG CAGCATTATT
    ACCGTCAAGG CACAGTTTGA GAAGCAGCTG TTAACTGAGA GGACTTTGAA GACACAGGCT
    GTAAACAAGT TAGCCGAAAT CATGAACCGC AAGTTACCCA CGAAGCGAGG GCCGGACACA
    GATGTACGAA GAAAGGAAAA GGAGAACCGC AAGCTGCAGT TGGACCTGAA ATCGGAGCGT
    GAGAAATTCT CTCAGCTTGT CTTCAAATAC CAGAGGGAAG TGAATGACAT GCAGGCGCTA
    ATAGCAGATG AGAACCAAGC CCGAGTCGAA CTGCAGATGG CGCTAGATAG CAAAGACAGT
    GACATAGAGC AGCTCCGGTC GCAACTGCAG TCTATGAACC TCGGCCTTGA CAGCACAAGT
    ATCGGCAGTG GACACGGTGA CACAGATGCT GATGATGGCT TTCCAGAATC CAGGCTGGAA
    GGATGGTTGT CGCTTCCTTT GCGCAACGCC AAAAAGTTTG GTTGGAATAA GAAGTACGTA
    GTGGTGAGCA GCAGGAAGAT CCTGTTTTAT GACAGTGAGC AGGATAAGGA GCTGTCCAAC
    CCTTCCATGG TGCTGGATAT TGATAAGTTG TTCCATGTGC GACCAGTTAC TCAGACGGAT
    GTGTATCGAG CCGATGCCAA AGAAATTCCA CGGATTTTCC AGATTCTATA CGCCAATGAA
    GGAGAAAGCA AGAAAGAACA AGAGTTCCAA GTGGACCCCT TAGAGAAATC CAATTACATA
    TGTCATAAAG GACATGAGTT TATCCCCACA CTCTACCATT TCCCCACCAG CTGTGATGCT
    TGTATGAAGC CCCTGTGGCA CATGTTCAAA CCCCCTGCTG CCCTGGAGTG TCGGCGCTGC
    CATATCAAGT GCCACAAGGA TCATATGGAC AAGAAAGAAG AGATAATAGC ACCTTGCAAA
    GTGAACTATG ATATTTCCAC CGCAAAGAAT CTACTATTAC TAGCCAACTC CACAGAAGAA
    CAGCAGAAGT GGGTGAGTCG ACTGGTGAAA AAGATCCCCA AGAAGCCCCC TGCTTCTGAC
    CATCAAGCTC GGTCCTCCCC CCGGCCTCCT GCTAAGGCGT CGCTAAACCA GTCCATGAGG
    CGGCCAAGCC GGCAGCTTCC TCCAAATAAA CCCAGATTAC TTGATCTGGC ATTTAAAGAC
    TGGGACTGGT CTCTTGATGA TGTAGAGGAT GATGATGAGG ATGTTTTTGA TTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10317
    Clone ID Related Accession (Same CDS sequence) XM_002942181.3 , XM_002942181.4
    Accession Version XM_002942181.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4122bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho-associated protein kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002942181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GCAGTCAGCG CACTTGCAAA AAGTCATTAA CAAGCTGGAA
    GAGCAGCTCC ATAATGAGAT GCAAACCAAA GACGAACTGG AGCAAAAGTT CAGAGCTGTA
    AATTTACGTT TGGAGAAGAC AGTGAAGGAG CTGGATGAAG AGGCCAATGC TAGGAAGAGC
    ATAGAATCGA CCACCAGGCA GCTGGAGCGT GAGAAGGCTC TTCTGCAGCA CAAGAGCACA
    GAGTACCAAA GAAAAGCAGA GAATGATGCC GACAGGAAGC GCAGCCTGGA AAATGAAGTT
    AATAGTTTAA AGGATCAGCT TGAAGATTTG AAAAGAAGAA ACCAAAATTC CCAAATATCA
    AATGAGAAGA TGAATCAGCT CCAAAGACAG CTGGATGAAG CCAACGCACA GCTTCGCACA
    GAGTCTGATG CAGCTGCCCG GCTGAGGAAG ACTCAGACCG AGATGTCCAA ACAAATACAG
    CAGCTAGAAA CCAACAACCG TGAGCTTCAG GATAAGACTT GCATGTTGGA GAATGCCAAG
    CTGAAACTAG AAAAGGACTT TATTAACCTG CAGTCGGCTT TGGAGTCAGA ACGGAGGGAC
    CGGACTCAGG GGTCAGAAGT AATAAGTGAC CTTCAAGGAC GAATATCTGT CTTGGAAGAG
    GATCTAAAGA AAGGCAAAGA GCTGCTAGCA AGAGCAGAAG CAGAAAAGCA GCAAATGCAT
    GAGGGGTTAG CCATTTTGGA GAAGGAAAAG AGCAATCTGG AAATCGATAT GACCTACAAA
    CTAAAAGCAC TACAGCAAAG CGTTGAGAAA GAGGAGGCTG AACATAAAGC CACAAAAGCT
    CGGCTGGCTG ACAAGAACAA AATTTACCAG TCCATTGAGG AGACCAAGTC AGAAGCAATG
    AAAGACATGG AGAAGAAGCT TCATGAGGAG CGCCTGGTTA AGCAGAGGCT AGAGAACAGC
    TTGCTGGAGA CAGAGAAACA ATACTCCATG TTGGACTGTG ACCTCAAACA AGCCAAGCAA
    AAGATCAACG AGTTGGAATC ACTTAAAGAC AAACTTAGCG AGGATGTTAA AAACTTAACG
    CTCAAAGCAG AACAAGAGGC ACAGAAGCGC AACCTGACGC ATAACGATCT GAAGATGCAG
    ATACAGCAAG TGAATAGCCT GAAGATGTCT GAGAAGCAAC TGAAGCAAGA GATCAACCAT
    CTGACTGAGA TAAAACTTAA CCTGGAGAAA CAGAACAACG AGTTGCGCAA AGAAAGAGTA
    GATGCGGATG GACAGATGAA GGAGCTTCAA GACCAACTGG AGGCTGAGCA GTATTTCTCA
    ACGCTGTACA AGACGCAGGT GCGGGAGCTG AAAGAGGAGT GTGAAGACAA GAGCAAGATG
    TGCAAAGAAG TGCAGCAGAA GGTCCAAGAG CTTCAGGATG AGAGAGACTC CCTGGCGGCT
    CAACTGGAGA TCACATTGAC CAAGGCCGAC TCCGAGCAGC TGGCCCGTTC CATTGCCGAG
    GAACAGTATT CCGACCTGGA GAAAGAGAAG ATCATGAAGG AACTAGAGAT TAAGGAGATG
    ATGGCACGGC ACAAGCAGGA GCTGGCAGAA AAATATGCTA CAATTGCTTC TTTGGAAGAA
    GCTAACAAAA CACTGACTAT CGATGTAGGC AATTTGGCTA ATGAGAAGGA AGACTTAAAC
    AACAAACTGA AAGAAACCCA AGAACTAATT CAGAATCTGA AAGAGGAAGA AAGCAGCATT
    ATTACCGTCA AGGCACAGTT TGAGAAGCAG CTGTTAACTG AGAGGACTTT GAAGACACAG
    GCTGTAAACA AGTTAGCCGA AATCATGAAC CGCAAGTTAC CCACGAAGCG AGGGCCGGAC
    ACAGATGTAC GAAGAAAGGA AAAGGAGAAC CGCAAGCTGC AGTTGGACCT GAAATCGGAG
    CGTGAGAAAT TCTCTCAGCT TGTCTTCAAA TACCAGAGGG AAGTGAATGA CATGCAGGCG
    CTAATAGCAG ATGAGAACCA AGCCCGAGTC GAACTGCAGA TGGCGCTAGA TAGCAAAGAC
    AGTGACATAG AGCAGCTCCG GTCGCAACTG CAGTCTATGA ACCTCGGCCT TGACAGCACA
    AGTATCGGCA GTGGACACGG TGACACAGAT GCTGATGATG GCTTTCCAGA ATCCAGGCTG
    GAAGGATGGT TGTCGCTTCC TTTGCGCAAC GCCAAAAAGT TTGGTTGGAA TAAGAAGTAC
    GTAGTGGTGA GCAGCAGGAA GATCCTGTTT TATGACAGTG AGCAGGATAA GGAGCTGTCC
    AACCCTTCCA TGGTGCTGGA TATTGATAAG TTGTTCCATG TGCGACCAGT TACTCAGACG
    GATGTGTATC GAGCCGATGC CAAAGAAATT CCACGGATTT TCCAGATTCT ATACGCCAAT
    GAAGGAGAAA GCAAGAAAGA ACAAGAGTTC CAAGTGGACC CCTTAGAGAA ATCCAATTAC
    ATATGTCATA AAGGACATGA GTTTATCCCC ACACTCTACC ATTTCCCCAC CAGCTGTGAT
    GCTTGTATGA AGCCCCTGTG GCACATGTTC AAACCCCCTG CTGCCCTGGA GTGTCGGCGC
    TGCCATATCA AGTGCCACAA GGATCATATG GACAAGAAAG AAGAGATAAT AGCACCTTGC
    AAAGTGAACT ATGATATTTC CACCGCAAAG AATCTACTAT TACTAGCCAA CTCCACAGAA
    GAACAGCAGA AGTGGGTGAG TCGACTGGTG AAAAAGATCC CCAAGAAGCC CCCTGCTTCT
    GACCATCAAG CTCGGTCCTC CCCCCGGCCT CCTGCTAAGG CGTCGCTAAA CCAGTCCATG
    AGGCGGCCAA GCCGGCAGCT TCCTCCAAAT AAACCCAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002942227.2
    CDS977..5098
    Translation

    Target ORF information:

    RefSeq Version XM_002942181.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho-associated, coiled-coil containing protein kinase 2 (rock2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002942181.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTCCCA CCCAGGAGGA GTGCATGAAC AGCCGGCGGC AGACGCTGGA AGCCATTCTG 
    CGAGACCCCC GCTCCCCTAT TAATGTTGAG GGCTTGCTGG ATGGGCTTAA CTCAATCGTC
    CTGGATCTTG ATTTCCCTGC ACTACGAAAG AACAAGAATA TAGATAACTT TTTAAATAGA
    TATGAAAAAA TTGTCAGGGA AGTCCGCAAG CTTCAGATGA AAGCCGAGGA CTATGACGTT
    GTCAAAGTCA TTGGAAGAGG TGCCTTTGGG GAAGTCCAGT TGGTTCGACA CAAAGGTTCT
    CAGAAGGTTT ACGCTATGAA ACTCCTTAGT AAATTTGAGA TGATCAAACG ATCTGACTCT
    GCCTTTTTTT GGGAAGAAAG AGATATCATG GCCTTTGCAA ATAGCCCTTG GGTAGTTCAG
    CTTTTTTGTG CCTTTCAAGA TGAGAAGCAC CTGTATATGG TGATGGAATA CATGCCAGGG
    GGCGACCTGG TTAACCTGAT GAGTAATTAC GACGTGCCTG AGAAATGGGC TAAGTTCTAC
    ACGGCGGAGG TTGTCCTGGC CCTCAATGCC ATTCACTCCA TGGGTTTGAT ACACAGGGAT
    GTGAAGCCTG ACAACATGCT CTTGGATAAG TACGGGCACC TGAAACTGGC AGACTTTGGC
    ACATGTATGA AGATGGACCA AACAGGCATG GTGCGCTGTG ATACCGCTGT TGGGACCCCA
    GACTACATAT CTCCCGAAGT GCTGAAATCT CAAGGAGGTG ATGGATACTA TGGCAGGGAA
    TGTGACTGGT GGTCTGTGGG GGTCTTCCTG TTTGAGATGC TAGTTGGGGA TACCCCATTT
    TATGCTGATT CACTGGTTGG AACTTACAGT AAAATTATGG ACCATAAGAA CTCGTTAAAC
    TTCCCTGAAG ATGTGGAGAT CTCAGTGCAT GCCAAGAACC TCATTTGTGC TTTCTTGACT
    GACAGGGAGG TCCGTCTCGG GAGGAACGGA ATCGAAGATA TAAAGCAGCA CCCTTTCTTC
    AAGAACGACC AGTGGAACTG GGACAACATT CGTGAAACGG TTGCACCTGT TGTGCCCGAG
    TTGGCCAGTG ACATCGACAC CAGTAACTTC GATGACATCG AAGATGACAA GGGAGACGCG
    GAAACGTTTC CTAACCCGAA GGCCTTTGCA GGAAACCAAC TGCCTTTTAT AGGTTTCACC
    TACTACCGTG AAAATCTATT ACTAAGCGAG TCTTCCCGAA ACTGTAAGGA GAAAAATTCA
    TCTGCCCAAC CAATGAAAGA GCAGTCAGCG CACTTGCAAA AAGTCATTAA CAAGCTGGAA
    GAGCAGCTCC ATAATGAGAT GCAAACCAAA GACGAACTGG AGCAAAAGTT CAGAGCTGTA
    AATTTACGTT TGGAGAAGAC AGTGAAGGAG CTGGATGAAG AGGCCAATGC TAGGAAGAGC
    ATAGAATCGA CCACCAGGCA GCTGGAGCGT GAGAAGGCTC TTCTGCAGCA CAAGAGCACA
    GAGTACCAAA GAAAAGCAGA GAATGATGCC GACAGGAAGC GCAGCCTGGA AAATGAAGTT
    AATAGTTTAA AGGATCAGCT TGAAGATTTG AAAAGAAGAA ACCAAAATTC CCAAATATCA
    AATGAGAAGA TGAATCAGCT CCAAAGACAG CTGGATGAAG CCAACGCACA GCTTCGCACA
    GAGTCTGATG CAGCTGCCCG GCTGAGGAAG ACTCAGACCG AGATGTCCAA ACAAATACAG
    CAGCTAGAAA CCAACAACCG TGAGCTTCAG GATAAGACTT GCATGTTGGA GAATGCCAAG
    CTGAAACTAG AAAAGGACTT TATTAACCTG CAGTCGGCTT TGGAGTCAGA ACGGAGGGAC
    CGGACTCAGG GGTCAGAAGT AATAAGTGAC CTTCAAGGAC GAATATCTGT CTTGGAAGAG
    GATCTAAAGA AAGGCAAAGA GCTGCTAGCA AGAGCAGAAG CAGAAAAGCA GCAAATGCAT
    GAGGGGTTAG CCATTTTGGA GAAGGAAAAG AGCAATCTGG AAATCGATAT GACCTACAAA
    CTAAAAGCAC TACAGCAAAG CGTTGAGAAA GAGGAGGCTG AACATAAAGC CACAAAAGCT
    CGGCTGGCTG ACAAGAACAA AATTTACCAG TCCATTGAGG AGACCAAGTC AGAAGCAATG
    AAAGACATGG AGAAGAAGCT TCATGAGGAG CGCCTGGTTA AGCAGAGGCT AGAGAACAGC
    TTGCTGGAGA CAGAGAAACA ATACTCCATG TTGGACTGTG ACCTCAAACA AGCCAAGCAA
    AAGATCAACG AGTTGGAATC ACTTAAAGAC AAACTTAGCG AGGATGTTAA AAACTTAACG
    CTCAAAGCAG AACAAGAGGC ACAGAAGCGC AACCTGACGC ATAACGATCT GAAGATGCAG
    ATACAGCAAG TGAATAGCCT GAAGATGTCT GAGAAGCAAC TGAAGCAAGA GATCAACCAT
    CTGACTGAGA TAAAACTTAA CCTGGAGAAA CAGAACAACG AGTTGCGCAA AGAAAGAGTA
    GATGCGGATG GACAGATGAA GGAGCTTCAA GACCAACTGG AGGCTGAGCA GTATTTCTCA
    ACGCTGTACA AGACGCAGGT GCGGGAGCTG AAAGAGGAGT GTGAAGACAA GAGCAAGATG
    TGCAAAGAAG TGCAGCAGAA GGTCCAAGAG CTTCAGGATG AGAGAGACTC CCTGGCGGCT
    CAACTGGAGA TCACATTGAC CAAGGCCGAC TCCGAGCAGC TGGCCCGTTC CATTGCCGAG
    GAACAGTATT CCGACCTGGA GAAAGAGAAG ATCATGAAGG AACTAGAGAT TAAGGAGATG
    ATGGCACGGC ACAAGCAGGA GCTGGCAGAA AAATATGCTA CAATTGCTTC TTTGGAAGAA
    GCTAACAAAA CACTGACTAT CGATGTAGGC AATTTGGCTA ATGAGAAGGA AGACTTAAAC
    AACAAACTGA AAGAAACCCA AGAACTAATT CAGAATCTGA AAGAGGAAGA AAGCAGCATT
    ATTACCGTCA AGGCACAGTT TGAGAAGCAG CTGTTAACTG AGAGGACTTT GAAGACACAG
    GCTGTAAACA AGTTAGCCGA AATCATGAAC CGCAAGTTAC CCACGAAGCG AGGGCCGGAC
    ACAGATGTAC GAAGAAAGGA AAAGGAGAAC CGCAAGCTGC AGTTGGACCT GAAATCGGAG
    CGTGAGAAAT TCTCTCAGCT TGTCTTCAAA TACCAGAGGG AAGTGAATGA CATGCAGGCG
    CTAATAGCAG ATGAGAACCA AGCCCGAGTC GAACTGCAGA TGGCGCTAGA TAGCAAAGAC
    AGTGACATAG AGCAGCTCCG GTCGCAACTG CAGTCTATGA ACCTCGGCCT TGACAGCACA
    AGTATCGGCA GTGGACACGG TGACACAGAT GCTGATGATG GCTTTCCAGA ATCCAGGCTG
    GAAGGATGGT TGTCGCTTCC TTTGCGCAAC GCCAAAAAGT TTGGTTGGAA TAAGAAGTAC
    GTAGTGGTGA GCAGCAGGAA GATCCTGTTT TATGACAGTG AGCAGGATAA GGAGCTGTCC
    AACCCTTCCA TGGTGCTGGA TATTGATAAG TTGTTCCATG TGCGACCAGT TACTCAGACG
    GATGTGTATC GAGCCGATGC CAAAGAAATT CCACGGATTT TCCAGATTCT ATACGCCAAT
    GAAGGAGAAA GCAAGAAAGA ACAAGAGTTC CAAGTGGACC CCTTAGAGAA ATCCAATTAC
    ATATGTCATA AAGGACATGA GTTTATCCCC ACACTCTACC ATTTCCCCAC CAGCTGTGAT
    GCTTGTATGA AGCCCCTGTG GCACATGTTC AAACCCCCTG CTGCCCTGGA GTGTCGGCGC
    TGCCATATCA AGTGCCACAA GGATCATATG GACAAGAAAG AAGAGATAAT AGCACCTTGC
    AAAGTGAACT ATGATATTTC CACCGCAAAG AATCTACTAT TACTAGCCAA CTCCACAGAA
    GAACAGCAGA AGTGGGTGAG TCGACTGGTG AAAAAGATCC CCAAGAAGCC CCCTGCTTCT
    GACCATCAAG CTCGGTCCTC CCCCCGGCCT CCTGCTAAGG CGTCGCTAAA CCAGTCCATG
    AGGCGGCCAA GCCGGCAGCT TCCTCCAAAT AAACCCAGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.