LOC100493774 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012967418.1	XP_012822872.1	myosin-10-like isoform X1
XM_012967417.2	XP_012822871.1	myosin-10-like isoform X1
XM_004916218.4	XP_004916275.2	myosin-10 isoform X1
XM_031905588.1	XP_031761448.1	myosin-10 isoform X2
XM_012967417.3	XP_012822871.2	myosin-10 isoform X1
XM_031905587.1	XP_031761447.1	myosin-10 isoform X1
XM_004916218.3	XP_004916275.1	myosin-10-like isoform X1
XM_018095487.1	XP_017950976.1	myosin-10-like isoform X2
XM_012967418.2	XP_012822872.2	myosin-10 isoform X1
XM_018095487.2	XP_017950976.2	myosin-10 isoform X3
XM_031905589.1	XP_031761449.1	myosin-10 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45450	XM_018095487.1 Latest version!	Xenopus tropicalis myosin-10-like (LOC100493774), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa61970	XM_012967418.2 Latest version!	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61970	XM_031905587.1 Latest version!	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61970	XM_004916218.4 Latest version!	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61970	XM_012967417.3 Latest version!	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61971	XM_031905588.1 Latest version!	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61972	XM_018095487.2 Latest version!	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa61973	XM_031905589.1 Latest version!	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa13288	XM_012967418.1 Latest version!	Xenopus tropicalis myosin-10-like (LOC100493774), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa13288	XM_012967417.2 Latest version!	Xenopus tropicalis myosin-10-like (LOC100493774), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa13288	XM_004916218.3 Latest version!	Xenopus tropicalis myosin-10-like (LOC100493774), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

LOC100493774 ( XM_018095487.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950976.1 Xenopus tropicalis myosin-10-like (LOC100493774), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa45450
Clone ID Related Accession (Same CDS sequence)	XM_018095487.1
Accession Version	XM_018095487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-10-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGTT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG TTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGACGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGGCG GGCACCTATA 
CAGTTCACCA CAAGAACTAT CCGCCAGGTG TATCAACTGG AAGCAGTTTC CGACGAGGAA 
CCCGAATCCC ACAGCGGAGA ACCATCTGCC AACCATCAGC AGCAGCAGCC TCCCCAGGCA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950976.1
CDS	180..6125
Translation	MSRNSYRDDAERYLFVDRNPTSVTATQADWTAKRQVWVPSEKHGFEAASIKEERGEEVIVELAENGKRVPVAKDDIQKMNPPKFTKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYTEQIVEMYRGKKRHEIPPHIYAISETAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKEHTAPSSSNTFYGELEHQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAIRQAKDERTFHVFYQLLAGAGEHMKTDLLLEGFNQYRFLSNGNLPIPGQQDREIFQETMESMKIMGFNHEEIMSMLKMVSAVLQFGNIVFRKERNTDQASMPDNTAAQKLCHLLGLNVTEFSRAILMPRIKVGRDYVQKAQTKEQADFAVEALAKALYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLSLLDEECWFPKATDKSFVEKVIQELGNHPKFQKPRQLRDKADLCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSTDKYTAELWKDVDRIVGLDQVSGMGEMSFGSSYKTKKGMFRTVGQLYKESLSKLMSTLRNTNPNFVRCIIPNHEKKAGKLEPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACAIMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIVFFQAAARGYLARRAFYKKQQQMSALKVVQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVMQAKVVELQKVKDTQVKTESELKEMANKYQQLFEEKSILAEQLQAETELFAEAEEMRARLASKKQELEEILHDLEARVEEEEERTLQLQNEKKKMHQHIQDLEEQLEEEEGARQKLQLEKVTTESRLKKMEEDILLLEDQNAKLAKERKLLDDRIGEFTSTMAEEEEKVKSLNKLRNKYEAVIADLEDRLKKEEKGRQEMEKMKRKLDGETTDLQDQLLELQQQIEELKQQLARKEEELQAALARVDDEVGQKNNLLKQLRDLQSQLAELHEDLESEKAARAKAEKQRRDLGEELEALKTELEDTLDSTAAQQELRAKREQEVTDLKKTIEEDVKVRDAQVTEMRQRHNQVVEEISEQLEQARRFKGNLEKVKQTLESENTDLIKEVKNLQAAKQDSEQRRKKLEQQVSEFQIRTNESEKVKFELAEKLQKLQAELDGVSGALGSTEGKSIKLTKDLSTVQSQLQDTQELLQEETRQKLNFSSRVRQLEEEKNNLMENLEEEESAKAQLSRQLQALQQQLLESKKRMEDQGGMVEAMEEAKKKSYKELEFLQQRFDEKHQINDKLEKTRNRLQQELDDLMVDLDHQRQIVSNLEKKQKKFDQMLAEEKNISARYGEERDRAEAEAREKETKALSLSRALEEAIDLKDELDRQNKQLRAEMDDLVSSKDDVGKNVHELERSKRALEQQVQEMKTQIEELEDELQAIEDGKLRLEVNMQAMKAQFERDLQNRDDSNDEKKKLLFKQVREMEVELEEERKQKSQILVAKKKLEMDLQDMESQMDSANKGRDEAVKQLKKLQLQFKEVWREVEETRAARDEIFVQSRDNEKKLKSLEAELLQLQEDLAAAERAKRQAQQERDDLADELSNGVSGKSALLDEKRALEMRISQLEEELDEEQSNTELINDRYRKLTLQVETITTELSAERSFSQKAENARQQMERQNKELKVKLNEMDSTMRSKYKITIASLEAKISQLEEQMEQESKERIIANKLVRRAEKRLKEVLLQVEEERRNADQFKEQLEKANIRMKQLKRQLEEAEEEASRANSNRRRLQRELEDVTESAESMNREVTTLRSRLRRAPIQFTTRTIRQVYQLEAVSDEEPESHSGEPSANHQQQQPPQAE

Target ORF information:

RefSeq Version	XM_018095487.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-10-like (LOC100493774), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095487.1

ORF Insert Sequence:

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGTT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG TTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGACGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGGCG GGCACCTATA 
CAGTTCACCA CAAGAACTAT CCGCCAGGTG TATCAACTGG AAGCAGTTTC CGACGAGGAA 
CCCGAATCCC ACAGCGGAGA ACCATCTGCC AACCATCAGC AGCAGCAGCC TCCCCAGGCA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493774 ( XM_004916218.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916275.2 Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X2, mRNA.

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CloneID	OXa61970
Clone ID Related Accession (Same CDS sequence)	XM_004916218.4 , XM_012967417.3 , XM_031905587.1 , XM_012967418.2
Accession Version	XM_004916218.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916218.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGCT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG CTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGATGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916275.2
CDS	124..6093
Translation	MSRNSYRDDAERYLFVDRNPTSVTATQADWTAKRQVWVPSEKHGFEAASIKEERGEEVIVELAENGKRVPVAKDDIQKMNPPKFTKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYTEQIVEMYRGKKRHEIPPHIYAISETAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKEHTAPSSSNTFYGELEHQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAIRQAKDERTFHVFYQLLAGAGEHMKTDLLLEGFNQYRFLSNGNLPIPGQQDREIFQETMESMKIMGFNHEEIMSMLKMVSAVLQFGNIVFRKERNTDQASMPDNTAAQKLCHLLGLNVTEFSRAILMPRIKVGRDYVQKAQTKEQADFAVEALAKALYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLSLLDEECWFPKATDKSFVEKVIQELGNHPKFQKPRQLRDKADLCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSTDKYTAELWKDVDRIVGLDQVSGMGEMSFGSSYKTKKGMFRTVGQLYKESLSKLMSTLRNTNPNFVRCIIPNHEKKAGKLEPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACAIMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIVFFQAAARGYLARRAFYKKQQQMSALKVVQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVMQAKVVELQKVKDTQVKTESELKEMANKYQQLFEEKSILAEQLQAETELFAEAEEMRARLASKKQELEEILHDLEARVEEEEERTLQLQNEKKKMHQHIQDLEEQLEEEEGARQKLQLEKVTTESRLKKMEEDILLLEDQNAKLAKERKLLDDRIGEFTSTMAEEEEKVKSLNKLRNKYEAVIADLEDRLKKEEKGRQEMEKMKRKLDGETTDLQDQLLELQQQIEELKQQLARKEEELQAALARVDDEVGQKNNLLKQLRDLQSQLAELHEDLESEKAARAKAEKQRRDLGEELEALKTELEDTLDSTAAQQELRAKREQEVTDLKKTIEEDVKVRDAQVTEMRQRHNQVVEEISEQLEQARRFKGNLEKVKQTLESENTDLIKEVKNLQAAKQDSEQRRKKLEQQVSEFQIRTNESEKVKFELAEKLQKLQAELDGVSGALGSTEGKSIKLTKDLSTVQSQLQDTQELLQEETRQKLNFSSRVRQLEEEKNNLMENLEEEESAKAQLSRQLQALQQQLLESKKRMEDQGGMVEAMEEAKKKSYKELEFLQQRFDEKHQINDKLEKTRNRLQQELDDLMVDLDHQRQIVSNLEKKQKKFDQMLAEEKNISARYGEERDRAEAEAREKETKALSLSRALEEAIDLKDELDRQNKQLRAEMDDLVSSKDDVGKNVHELERSKRALEQQVQEMKTQIEELEDELQAIEDGKLRLEVNMQAMKAQFERDLQNRDDSNDEKKKLLFKQVREMEVELEEERKQKSQILAAKKKLEMDLQDMESQMDSANKGRDEAVKQLKKLQLQFKEVWREVEETRAARDEIFVQSRDNEKKLKSLEAELLQLQEDLAAAERAKRQAQQERDDLADELSNGVSGKSALLDEKRALEMRISQLEEELDEEQSNTELINDRYRKLTLQVETITTELSAERSFSQKAENARQQMERQNKELKVKLNEMDSTMRSKYKITIASLEAKISQLEEQMEQESKERIIANKLVRRAEKRLKEVLLQVEEERRNADQFKEQLEKANIRMKQLKRQLEEAEEEASRANSNRRRLQRELEDVTESAESMNREVTTLRSRLSKLERQQRKRAPIQFTTRTIRQVYQLEAVSDEEPESHSGEPSANHQQQQPPQAE

Target ORF information:

RefSeq Version	XM_004916218.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916218.4

ORF Insert Sequence:

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGCT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG CTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGATGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493774 ( XM_012967417.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822871.2 Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X1, mRNA.

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CloneID	OXa61970
Clone ID Related Accession (Same CDS sequence)	XM_004916218.4 , XM_012967417.3 , XM_031905587.1 , XM_012967418.2
Accession Version	XM_012967417.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967417.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGCT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG CTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGATGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822871.2
CDS	181..6150
Translation	MSRNSYRDDAERYLFVDRNPTSVTATQADWTAKRQVWVPSEKHGFEAASIKEERGEEVIVELAENGKRVPVAKDDIQKMNPPKFTKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYTEQIVEMYRGKKRHEIPPHIYAISETAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKEHTAPSSSNTFYGELEHQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAIRQAKDERTFHVFYQLLAGAGEHMKTDLLLEGFNQYRFLSNGNLPIPGQQDREIFQETMESMKIMGFNHEEIMSMLKMVSAVLQFGNIVFRKERNTDQASMPDNTAAQKLCHLLGLNVTEFSRAILMPRIKVGRDYVQKAQTKEQADFAVEALAKALYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLSLLDEECWFPKATDKSFVEKVIQELGNHPKFQKPRQLRDKADLCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSTDKYTAELWKDVDRIVGLDQVSGMGEMSFGSSYKTKKGMFRTVGQLYKESLSKLMSTLRNTNPNFVRCIIPNHEKKAGKLEPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACAIMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIVFFQAAARGYLARRAFYKKQQQMSALKVVQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVMQAKVVELQKVKDTQVKTESELKEMANKYQQLFEEKSILAEQLQAETELFAEAEEMRARLASKKQELEEILHDLEARVEEEEERTLQLQNEKKKMHQHIQDLEEQLEEEEGARQKLQLEKVTTESRLKKMEEDILLLEDQNAKLAKERKLLDDRIGEFTSTMAEEEEKVKSLNKLRNKYEAVIADLEDRLKKEEKGRQEMEKMKRKLDGETTDLQDQLLELQQQIEELKQQLARKEEELQAALARVDDEVGQKNNLLKQLRDLQSQLAELHEDLESEKAARAKAEKQRRDLGEELEALKTELEDTLDSTAAQQELRAKREQEVTDLKKTIEEDVKVRDAQVTEMRQRHNQVVEEISEQLEQARRFKGNLEKVKQTLESENTDLIKEVKNLQAAKQDSEQRRKKLEQQVSEFQIRTNESEKVKFELAEKLQKLQAELDGVSGALGSTEGKSIKLTKDLSTVQSQLQDTQELLQEETRQKLNFSSRVRQLEEEKNNLMENLEEEESAKAQLSRQLQALQQQLLESKKRMEDQGGMVEAMEEAKKKSYKELEFLQQRFDEKHQINDKLEKTRNRLQQELDDLMVDLDHQRQIVSNLEKKQKKFDQMLAEEKNISARYGEERDRAEAEAREKETKALSLSRALEEAIDLKDELDRQNKQLRAEMDDLVSSKDDVGKNVHELERSKRALEQQVQEMKTQIEELEDELQAIEDGKLRLEVNMQAMKAQFERDLQNRDDSNDEKKKLLFKQVREMEVELEEERKQKSQILAAKKKLEMDLQDMESQMDSANKGRDEAVKQLKKLQLQFKEVWREVEETRAARDEIFVQSRDNEKKLKSLEAELLQLQEDLAAAERAKRQAQQERDDLADELSNGVSGKSALLDEKRALEMRISQLEEELDEEQSNTELINDRYRKLTLQVETITTELSAERSFSQKAENARQQMERQNKELKVKLNEMDSTMRSKYKITIASLEAKISQLEEQMEQESKERIIANKLVRRAEKRLKEVLLQVEEERRNADQFKEQLEKANIRMKQLKRQLEEAEEEASRANSNRRRLQRELEDVTESAESMNREVTTLRSRLSKLERQQRKRAPIQFTTRTIRQVYQLEAVSDEEPESHSGEPSANHQQQQPPQAE

Target ORF information:

RefSeq Version	XM_012967417.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967417.3

ORF Insert Sequence:

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGCT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG CTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGATGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493774 ( XM_031905587.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761447.1 Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X4, mRNA.

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CloneID	OXa61970
Clone ID Related Accession (Same CDS sequence)	XM_004916218.4 , XM_012967417.3 , XM_031905587.1 , XM_012967418.2
Accession Version	XM_031905587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGCT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG CTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGATGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761447.1
CDS	126..6095
Translation	MSRNSYRDDAERYLFVDRNPTSVTATQADWTAKRQVWVPSEKHGFEAASIKEERGEEVIVELAENGKRVPVAKDDIQKMNPPKFTKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYTEQIVEMYRGKKRHEIPPHIYAISETAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKEHTAPSSSNTFYGELEHQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAIRQAKDERTFHVFYQLLAGAGEHMKTDLLLEGFNQYRFLSNGNLPIPGQQDREIFQETMESMKIMGFNHEEIMSMLKMVSAVLQFGNIVFRKERNTDQASMPDNTAAQKLCHLLGLNVTEFSRAILMPRIKVGRDYVQKAQTKEQADFAVEALAKALYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLSLLDEECWFPKATDKSFVEKVIQELGNHPKFQKPRQLRDKADLCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSTDKYTAELWKDVDRIVGLDQVSGMGEMSFGSSYKTKKGMFRTVGQLYKESLSKLMSTLRNTNPNFVRCIIPNHEKKAGKLEPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACAIMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIVFFQAAARGYLARRAFYKKQQQMSALKVVQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVMQAKVVELQKVKDTQVKTESELKEMANKYQQLFEEKSILAEQLQAETELFAEAEEMRARLASKKQELEEILHDLEARVEEEEERTLQLQNEKKKMHQHIQDLEEQLEEEEGARQKLQLEKVTTESRLKKMEEDILLLEDQNAKLAKERKLLDDRIGEFTSTMAEEEEKVKSLNKLRNKYEAVIADLEDRLKKEEKGRQEMEKMKRKLDGETTDLQDQLLELQQQIEELKQQLARKEEELQAALARVDDEVGQKNNLLKQLRDLQSQLAELHEDLESEKAARAKAEKQRRDLGEELEALKTELEDTLDSTAAQQELRAKREQEVTDLKKTIEEDVKVRDAQVTEMRQRHNQVVEEISEQLEQARRFKGNLEKVKQTLESENTDLIKEVKNLQAAKQDSEQRRKKLEQQVSEFQIRTNESEKVKFELAEKLQKLQAELDGVSGALGSTEGKSIKLTKDLSTVQSQLQDTQELLQEETRQKLNFSSRVRQLEEEKNNLMENLEEEESAKAQLSRQLQALQQQLLESKKRMEDQGGMVEAMEEAKKKSYKELEFLQQRFDEKHQINDKLEKTRNRLQQELDDLMVDLDHQRQIVSNLEKKQKKFDQMLAEEKNISARYGEERDRAEAEAREKETKALSLSRALEEAIDLKDELDRQNKQLRAEMDDLVSSKDDVGKNVHELERSKRALEQQVQEMKTQIEELEDELQAIEDGKLRLEVNMQAMKAQFERDLQNRDDSNDEKKKLLFKQVREMEVELEEERKQKSQILAAKKKLEMDLQDMESQMDSANKGRDEAVKQLKKLQLQFKEVWREVEETRAARDEIFVQSRDNEKKLKSLEAELLQLQEDLAAAERAKRQAQQERDDLADELSNGVSGKSALLDEKRALEMRISQLEEELDEEQSNTELINDRYRKLTLQVETITTELSAERSFSQKAENARQQMERQNKELKVKLNEMDSTMRSKYKITIASLEAKISQLEEQMEQESKERIIANKLVRRAEKRLKEVLLQVEEERRNADQFKEQLEKANIRMKQLKRQLEEAEEEASRANSNRRRLQRELEDVTESAESMNREVTTLRSRLSKLERQQRKRAPIQFTTRTIRQVYQLEAVSDEEPESHSGEPSANHQQQQPPQAE

Target ORF information:

RefSeq Version	XM_031905587.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905587.1

ORF Insert Sequence:

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGCT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG CTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGATGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493774 ( XM_012967418.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822872.2 Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X3, mRNA.

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CloneID	OXa61970
Clone ID Related Accession (Same CDS sequence)	XM_004916218.4 , XM_012967417.3 , XM_031905587.1 , XM_012967418.2
Accession Version	XM_012967418.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967418.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGCT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG CTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGATGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822872.2
CDS	342..6311
Translation	MSRNSYRDDAERYLFVDRNPTSVTATQADWTAKRQVWVPSEKHGFEAASIKEERGEEVIVELAENGKRVPVAKDDIQKMNPPKFTKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYTEQIVEMYRGKKRHEIPPHIYAISETAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKEHTAPSSSNTFYGELEHQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAIRQAKDERTFHVFYQLLAGAGEHMKTDLLLEGFNQYRFLSNGNLPIPGQQDREIFQETMESMKIMGFNHEEIMSMLKMVSAVLQFGNIVFRKERNTDQASMPDNTAAQKLCHLLGLNVTEFSRAILMPRIKVGRDYVQKAQTKEQADFAVEALAKALYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLSLLDEECWFPKATDKSFVEKVIQELGNHPKFQKPRQLRDKADLCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSTDKYTAELWKDVDRIVGLDQVSGMGEMSFGSSYKTKKGMFRTVGQLYKESLSKLMSTLRNTNPNFVRCIIPNHEKKAGKLEPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACAIMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIVFFQAAARGYLARRAFYKKQQQMSALKVVQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVMQAKVVELQKVKDTQVKTESELKEMANKYQQLFEEKSILAEQLQAETELFAEAEEMRARLASKKQELEEILHDLEARVEEEEERTLQLQNEKKKMHQHIQDLEEQLEEEEGARQKLQLEKVTTESRLKKMEEDILLLEDQNAKLAKERKLLDDRIGEFTSTMAEEEEKVKSLNKLRNKYEAVIADLEDRLKKEEKGRQEMEKMKRKLDGETTDLQDQLLELQQQIEELKQQLARKEEELQAALARVDDEVGQKNNLLKQLRDLQSQLAELHEDLESEKAARAKAEKQRRDLGEELEALKTELEDTLDSTAAQQELRAKREQEVTDLKKTIEEDVKVRDAQVTEMRQRHNQVVEEISEQLEQARRFKGNLEKVKQTLESENTDLIKEVKNLQAAKQDSEQRRKKLEQQVSEFQIRTNESEKVKFELAEKLQKLQAELDGVSGALGSTEGKSIKLTKDLSTVQSQLQDTQELLQEETRQKLNFSSRVRQLEEEKNNLMENLEEEESAKAQLSRQLQALQQQLLESKKRMEDQGGMVEAMEEAKKKSYKELEFLQQRFDEKHQINDKLEKTRNRLQQELDDLMVDLDHQRQIVSNLEKKQKKFDQMLAEEKNISARYGEERDRAEAEAREKETKALSLSRALEEAIDLKDELDRQNKQLRAEMDDLVSSKDDVGKNVHELERSKRALEQQVQEMKTQIEELEDELQAIEDGKLRLEVNMQAMKAQFERDLQNRDDSNDEKKKLLFKQVREMEVELEEERKQKSQILAAKKKLEMDLQDMESQMDSANKGRDEAVKQLKKLQLQFKEVWREVEETRAARDEIFVQSRDNEKKLKSLEAELLQLQEDLAAAERAKRQAQQERDDLADELSNGVSGKSALLDEKRALEMRISQLEEELDEEQSNTELINDRYRKLTLQVETITTELSAERSFSQKAENARQQMERQNKELKVKLNEMDSTMRSKYKITIASLEAKISQLEEQMEQESKERIIANKLVRRAEKRLKEVLLQVEEERRNADQFKEQLEKANIRMKQLKRQLEEAEEEASRANSNRRRLQRELEDVTESAESMNREVTTLRSRLSKLERQQRKRAPIQFTTRTIRQVYQLEAVSDEEPESHSGEPSANHQQQQPPQAE

Target ORF information:

RefSeq Version	XM_012967418.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967418.2

ORF Insert Sequence:

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGCT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG CTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGATGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493774 ( XM_031905588.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761448.1 Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X5, mRNA.

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CloneID	OXa61971
Clone ID Related Accession (Same CDS sequence)	XM_031905588.1
Accession Version	XM_031905588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGGGAGAGC TAGAGCACCA GCTCCTGCAG 
GCCAACCCGA TATTGGAAGC TTTTGGCAAT GCCAAGACTG TAAAAAATGA CAACTCTTCT 
CGATTTGGTA AATTTATCCG CATTAATTTT GATGTTGCTG GTTACATCGT TGGAGCCAAC 
ATTGAGACTT ATTTGTTGGA GAAGTCTCGT GCTATTCGAC AAGCTAAAGA CGAAAGAACA 
TTCCATGTTT TTTACCAGCT TCTAGCAGGA GCAGGGGAGC ACATGAAAAC CGACTTGCTT 
CTGGAGGGCT TCAATCAGTA CCGTTTCTTG TCTAATGGAA ACCTGCCCAT TCCTGGGCAG 
CAGGACAGGG AGATCTTCCA AGAGACAATG GAGTCCATGA AAATCATGGG ATTTAACCAT 
GAAGAAATCA TGTCAATGCT GAAAATGGTG TCAGCGGTTC TGCAGTTTGG CAATATAGTT 
TTCCGGAAAG AAAGGAACAC AGATCAAGCA TCAATGCCAG ATAACACAGC TGCTCAGAAA 
CTGTGCCACC TCCTGGGACT CAATGTCACA GAGTTTAGTC GTGCCATCCT GATGCCAAGG 
ATTAAAGTTG GCCGTGATTA TGTCCAGAAG GCCCAGACTA AAGAACAGGC GGACTTTGCT 
GTGGAAGCCC TGGCAAAAGC TCTATATGAA AGACTGTTCC GCTGGTTGGT TCACCGCATT 
AACAAGGCTC TGGACAGGAC CAAAAGGCAA GGCGCCTCTT TCATAGGCAT CCTGGATATC 
GCTGGCTTTG AAATCTTTGA GCTGAACTCC TTTGAACAGC TGTGCATCAA TTACACAAAT 
GAGAAGCTTC AGCAACTGTT CAACCACACA ATGTTTGTTC TGGAGCAGGA GGAATATCAG 
CGTGAGGGCA TTGAGTGGAA CTTTATTGAC TTTGGGCTTG ATCTGCAACC CTGTATTGAC 
CTCATTGAGA GACCGGCAAA CCCTCCAGGT GTCCTGTCCC TGCTGGATGA GGAGTGCTGG 
TTCCCCAAAG CCACTGATAA GAGCTTTGTT GAAAAAGTTA TCCAGGAATT GGGAAATCAC 
CCTAAATTCC AGAAACCTCG ACAATTGCGA GACAAAGCTG ATTTGTGCAT CATTCATTAT 
GCTGGGAAGG TGGATTACAA AGCAGATGAA TGGCTGATGA AGAACATGGA TCCACTGAAT 
GACAATGTGG CCACCCTGCT GCACCAAAGC ACTGACAAGT ATACCGCTGA GCTTTGGAAA 
GATGTGGATC GGATCGTGGG CCTGGACCAA GTAAGTGGAA TGGGAGAGAT GTCTTTTGGC 
TCATCATACA AAACCAAGAA GGGAATGTTT CGCACAGTGG GCCAGCTCTA CAAAGAGTCC 
CTGTCCAAGC TTATGTCTAC TCTCAGAAAC ACCAACCCCA ACTTTGTACG GTGCATTATA 
CCCAATCATG AGAAGAAGGC TGGAAAGCTG GAGCCTCACC TTGTACTTGA CCAGCTACGC 
TGTAATGGTG TCCTGGAGGG AATCCGTATT TGCAGACAGG GCTTCCCAAA TCGAATTGTA 
TTCCAAGAGT TTAGGCAAAG GTATGAGATT CTGACCCCAA ATGCCATTCC CAAAGGATTC 
ATGGATGGCA AGCAAGCTTG TGCAATAATG ATCAGAGCTT TGGAGCTTGA CCCTAACCTG 
TACCGCATAG GACAAAGTAA AATCTTCTTC CGAGCTGGAG TACTGGCACA CTTGGAAGAG 
GAAAGAGACC TAAAGATCAC TGACATTATT GTATTTTTCC AAGCTGCTGC TCGGGGTTAC 
CTGGCCAGGA GGGCATTTTA TAAGAAGCAG CAGCAGATGA GTGCTCTAAA AGTGGTGCAG 
CGGAATTGCG CCGCCTACCT CAAGCTCAGA CACTGGCAGT GGTGGAGGCT CTTCACCAAG 
GTAAAGCCAT TACTGCAAGT AACCCGTCAG GATGAGGTCA TGCAAGCAAA GGTTGTAGAA 
CTGCAAAAGG TGAAAGACAC CCAAGTAAAG ACTGAATCAG AGCTGAAGGA AATGGCTAAC 
AAGTACCAGC AGTTGTTTGA AGAGAAGAGC ATCTTGGCTG AGCAGTTACA AGCAGAAACA 
GAGCTTTTTG CCGAAGCAGA AGAAATGCGC GCCAGACTTG CATCCAAAAA GCAGGAATTG 
GAAGAGATTT TGCATGACTT GGAGGCTCGT GTGGAAGAGG AGGAAGAACG TACCTTGCAG 
CTACAAAACG AGAAGAAAAA GATGCATCAA CATATACAGG ATTTGGAAGA ACAGCTGGAG 
GAAGAAGAAG GAGCTCGACA GAAACTACAG CTAGAAAAAG TAACCACAGA GTCTAGGCTG 
AAGAAAATGG AAGAAGATAT ACTCTTACTG GAAGACCAGA ATGCCAAACT GGCCAAGGAG 
AGGAAACTGC TGGATGACCG CATTGGTGAA TTCACTTCAA CTATGGCTGA AGAAGAGGAG 
AAAGTGAAGA GCTTGAATAA ATTGCGTAAC AAATACGAAG CCGTCATTGC TGACCTGGAA 
GATCGTCTTA AAAAGGAAGA AAAGGGAAGG CAAGAGATGG AGAAGATGAA GAGGAAGCTG 
GATGGTGAAA CCACTGATTT ACAGGATCAG CTGCTAGAGC TGCAGCAGCA GATAGAGGAA 
CTAAAGCAAC AGCTGGCTCG TAAGGAAGAA GAACTCCAAG CTGCTCTTGC AAGAGTTGAT 
GATGAAGTAG GACAAAAGAA CAATTTGCTC AAACAACTTC GAGACTTGCA GTCACAGCTC 
GCTGAGTTAC ATGAAGATCT AGAGTCAGAG AAGGCTGCAC GAGCAAAGGC AGAAAAGCAG 
CGTCGGGACC TTGGTGAGGA GTTGGAGGCC CTGAAAACAG AGCTGGAAGA CACTCTCGAC 
TCCACTGCTG CACAGCAAGA GCTCAGAGCC AAAAGGGAAC AAGAGGTGAC AGACCTGAAG 
AAAACAATAG AGGAAGACGT AAAAGTTCGA GATGCCCAAG TAACAGAAAT GCGCCAGCGA 
CACAACCAAG TGGTGGAAGA GATCTCTGAA CAGCTGGAGC AGGCACGAAG GTTCAAGGGG 
AATCTGGAGA AGGTAAAGCA GACCCTGGAG AGTGAGAACA CTGACCTCAT TAAGGAAGTC 
AAGAATCTTC AAGCAGCTAA ACAGGATTCA GAACAACGCA GGAAGAAGCT GGAGCAGCAG 
GTGTCGGAAT TTCAGATCCG CACCAACGAA AGTGAAAAGG TCAAGTTCGA ACTGGCGGAG 
AAACTGCAGA AACTGCAGGC TGAATTAGAT GGTGTATCGG GCGCCCTTGG ATCAACAGAA 
GGCAAGTCAA TCAAACTAAC CAAAGACTTG TCAACAGTGC AGTCCCAACT GCAGGATACC 
CAGGAGTTGC TTCAGGAGGA AACAAGACAA AAGTTGAATT TTAGCTCTCG AGTTAGGCAG 
CTGGAGGAGG AAAAGAATAA CCTTATGGAG AACCTAGAGG AAGAGGAATC TGCTAAGGCC 
CAGCTAAGCC GCCAGTTACA GGCTTTGCAA CAACAGCTTC TCGAGTCTAA GAAGAGGATG 
GAGGACCAGG GTGGTATGGT GGAGGCTATG GAGGAAGCCA AGAAGAAATC ATATAAAGAA 
TTAGAGTTCC TCCAGCAACG CTTTGACGAG AAACATCAAA TAAACGATAA GCTAGAGAAG 
ACAAGAAACC GCTTACAGCA GGAGCTGGAT GACTTGATGG TGGATTTGGA CCATCAACGG 
CAAATAGTCT CCAATCTGGA GAAAAAGCAG AAGAAGTTTG ACCAGATGCT AGCTGAAGAG 
AAAAACATCT CTGCACGTTA TGGAGAAGAG CGAGATCGTG CTGAGGCCGA GGCTCGTGAG 
AAGGAAACAA AGGCCCTGTC TCTAAGTCGG GCCCTGGAAG AGGCCATTGA CCTAAAAGAT 
GAGCTGGACA GGCAGAACAA GCAATTAAGA GCAGAGATGG ATGATCTAGT TAGCTCCAAA 
GATGATGTTG GCAAGAATGT TCATGAGCTG GAAAGGTCTA AACGGGCCTT GGAACAGCAA 
GTTCAGGAAA TGAAGACCCA GATTGAGGAA CTGGAGGATG AGCTACAGGC CATTGAAGAT 
GGCAAACTTC GCTTGGAGGT CAATATGCAA GCAATGAAAG CTCAGTTTGA AAGAGACCTC 
CAAAACCGAG ATGATTCCAA TGATGAAAAG AAGAAGCTGC TCTTCAAACA GGTACGAGAG 
ATGGAAGTGG AGTTGGAAGA GGAAAGGAAA CAGAAATCCC AGATTCTTGC TGCCAAGAAA 
AAGCTTGAAA TGGATCTACA GGATATGGAA AGCCAGATGG ACTCTGCTAA CAAAGGTCGG 
GATGAAGCTG TTAAACAGCT GAAAAAACTA CAGCTCCAGT TCAAAGAAGT GTGGCGTGAA 
GTGGAGGAGA CCAGGGCAGC ACGAGATGAG ATCTTTGTTC AGTCTAGGGA TAATGAGAAG 
AAACTGAAGA GCCTGGAGGC TGAACTGCTT CAACTACAAG AGGACCTGGC AGCCGCCGAA 
CGTGCAAAGA GACAAGCTCA GCAGGAGAGA GACGACCTGG CAGATGAGCT GTCCAATGGA 
GTCAGTGGCA AATCGGCCCT GCTGGATGAG AAGAGAGCCC TGGAGATGCG TATCTCTCAG 
CTGGAAGAGG AGCTGGATGA AGAGCAGAGT AATACAGAGC TTATTAATGA CAGATACAGG 
AAATTAACTT TGCAGGTGGA GACAATAACC ACTGAGCTCT CAGCCGAGCG AAGCTTTTCT 
CAGAAGGCAG AGAATGCCCG TCAACAAATG GAGAGGCAGA ATAAAGAACT TAAGGTGAAA 
CTGAATGAGA TGGATTCCAC CATGAGGTCG AAGTACAAGA TCACCATCGC CTCTCTTGAG 
GCCAAGATTA GCCAGCTGGA AGAGCAGATG GAGCAGGAGT CTAAGGAACG TATCATAGCC 
AATAAGCTGG TCCGACGTGC TGAAAAGCGG CTTAAGGAGG TGCTGCTGCA GGTGGAAGAG 
GAAAGACGGA ATGCAGATCA GTTCAAGGAG CAGCTGGAGA AGGCAAATAT ACGCATGAAG 
CAGCTGAAGC GCCAATTGGA GGAGGCCGAG GAAGAGGCTT CACGCGCCAA CTCCAACCGC 
CGCCGGCTGC AGAGAGAGCT GGAAGATGTG ACTGAATCAG CAGAGTCCAT GAACCGAGAA 
GTGACCACCC TGAGAAGCAG GCTTAGCAAA TTAGAGCGTC AGCAACGCAA GCGGGCACCT 
ATACAGTTCA CCACAAGAAC TATCCGCCAG GTGTATCAAC TGGAAGCAGT TTCCGACGAG 
GAACCCGAAT CCCACAGCGG AGAACCATCT GCCAACCATC AGCAGCAGCA GCCTCCCCAG 
GCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761448.1
CDS	181..6129
Translation	MSRNSYRDDAERYLFVDRNPTSVTATQADWTAKRQVWVPSEKHGFEAASIKEERGEEVIVELAENGKRVPVAKDDIQKMNPPKFTKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYTEQIVEMYRGKKRHEIPPHIYAISETAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKEHTAPGELEHQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAIRQAKDERTFHVFYQLLAGAGEHMKTDLLLEGFNQYRFLSNGNLPIPGQQDREIFQETMESMKIMGFNHEEIMSMLKMVSAVLQFGNIVFRKERNTDQASMPDNTAAQKLCHLLGLNVTEFSRAILMPRIKVGRDYVQKAQTKEQADFAVEALAKALYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLSLLDEECWFPKATDKSFVEKVIQELGNHPKFQKPRQLRDKADLCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSTDKYTAELWKDVDRIVGLDQVSGMGEMSFGSSYKTKKGMFRTVGQLYKESLSKLMSTLRNTNPNFVRCIIPNHEKKAGKLEPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACAIMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIVFFQAAARGYLARRAFYKKQQQMSALKVVQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVMQAKVVELQKVKDTQVKTESELKEMANKYQQLFEEKSILAEQLQAETELFAEAEEMRARLASKKQELEEILHDLEARVEEEEERTLQLQNEKKKMHQHIQDLEEQLEEEEGARQKLQLEKVTTESRLKKMEEDILLLEDQNAKLAKERKLLDDRIGEFTSTMAEEEEKVKSLNKLRNKYEAVIADLEDRLKKEEKGRQEMEKMKRKLDGETTDLQDQLLELQQQIEELKQQLARKEEELQAALARVDDEVGQKNNLLKQLRDLQSQLAELHEDLESEKAARAKAEKQRRDLGEELEALKTELEDTLDSTAAQQELRAKREQEVTDLKKTIEEDVKVRDAQVTEMRQRHNQVVEEISEQLEQARRFKGNLEKVKQTLESENTDLIKEVKNLQAAKQDSEQRRKKLEQQVSEFQIRTNESEKVKFELAEKLQKLQAELDGVSGALGSTEGKSIKLTKDLSTVQSQLQDTQELLQEETRQKLNFSSRVRQLEEEKNNLMENLEEEESAKAQLSRQLQALQQQLLESKKRMEDQGGMVEAMEEAKKKSYKELEFLQQRFDEKHQINDKLEKTRNRLQQELDDLMVDLDHQRQIVSNLEKKQKKFDQMLAEEKNISARYGEERDRAEAEAREKETKALSLSRALEEAIDLKDELDRQNKQLRAEMDDLVSSKDDVGKNVHELERSKRALEQQVQEMKTQIEELEDELQAIEDGKLRLEVNMQAMKAQFERDLQNRDDSNDEKKKLLFKQVREMEVELEEERKQKSQILAAKKKLEMDLQDMESQMDSANKGRDEAVKQLKKLQLQFKEVWREVEETRAARDEIFVQSRDNEKKLKSLEAELLQLQEDLAAAERAKRQAQQERDDLADELSNGVSGKSALLDEKRALEMRISQLEEELDEEQSNTELINDRYRKLTLQVETITTELSAERSFSQKAENARQQMERQNKELKVKLNEMDSTMRSKYKITIASLEAKISQLEEQMEQESKERIIANKLVRRAEKRLKEVLLQVEEERRNADQFKEQLEKANIRMKQLKRQLEEAEEEASRANSNRRRLQRELEDVTESAESMNREVTTLRSRLSKLERQQRKRAPIQFTTRTIRQVYQLEAVSDEEPESHSGEPSANHQQQQPPQAE

Target ORF information:

RefSeq Version	XM_031905588.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905588.1

ORF Insert Sequence:

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGGGAGAGC TAGAGCACCA GCTCCTGCAG 
GCCAACCCGA TATTGGAAGC TTTTGGCAAT GCCAAGACTG TAAAAAATGA CAACTCTTCT 
CGATTTGGTA AATTTATCCG CATTAATTTT GATGTTGCTG GTTACATCGT TGGAGCCAAC 
ATTGAGACTT ATTTGTTGGA GAAGTCTCGT GCTATTCGAC AAGCTAAAGA CGAAAGAACA 
TTCCATGTTT TTTACCAGCT TCTAGCAGGA GCAGGGGAGC ACATGAAAAC CGACTTGCTT 
CTGGAGGGCT TCAATCAGTA CCGTTTCTTG TCTAATGGAA ACCTGCCCAT TCCTGGGCAG 
CAGGACAGGG AGATCTTCCA AGAGACAATG GAGTCCATGA AAATCATGGG ATTTAACCAT 
GAAGAAATCA TGTCAATGCT GAAAATGGTG TCAGCGGTTC TGCAGTTTGG CAATATAGTT 
TTCCGGAAAG AAAGGAACAC AGATCAAGCA TCAATGCCAG ATAACACAGC TGCTCAGAAA 
CTGTGCCACC TCCTGGGACT CAATGTCACA GAGTTTAGTC GTGCCATCCT GATGCCAAGG 
ATTAAAGTTG GCCGTGATTA TGTCCAGAAG GCCCAGACTA AAGAACAGGC GGACTTTGCT 
GTGGAAGCCC TGGCAAAAGC TCTATATGAA AGACTGTTCC GCTGGTTGGT TCACCGCATT 
AACAAGGCTC TGGACAGGAC CAAAAGGCAA GGCGCCTCTT TCATAGGCAT CCTGGATATC 
GCTGGCTTTG AAATCTTTGA GCTGAACTCC TTTGAACAGC TGTGCATCAA TTACACAAAT 
GAGAAGCTTC AGCAACTGTT CAACCACACA ATGTTTGTTC TGGAGCAGGA GGAATATCAG 
CGTGAGGGCA TTGAGTGGAA CTTTATTGAC TTTGGGCTTG ATCTGCAACC CTGTATTGAC 
CTCATTGAGA GACCGGCAAA CCCTCCAGGT GTCCTGTCCC TGCTGGATGA GGAGTGCTGG 
TTCCCCAAAG CCACTGATAA GAGCTTTGTT GAAAAAGTTA TCCAGGAATT GGGAAATCAC 
CCTAAATTCC AGAAACCTCG ACAATTGCGA GACAAAGCTG ATTTGTGCAT CATTCATTAT 
GCTGGGAAGG TGGATTACAA AGCAGATGAA TGGCTGATGA AGAACATGGA TCCACTGAAT 
GACAATGTGG CCACCCTGCT GCACCAAAGC ACTGACAAGT ATACCGCTGA GCTTTGGAAA 
GATGTGGATC GGATCGTGGG CCTGGACCAA GTAAGTGGAA TGGGAGAGAT GTCTTTTGGC 
TCATCATACA AAACCAAGAA GGGAATGTTT CGCACAGTGG GCCAGCTCTA CAAAGAGTCC 
CTGTCCAAGC TTATGTCTAC TCTCAGAAAC ACCAACCCCA ACTTTGTACG GTGCATTATA 
CCCAATCATG AGAAGAAGGC TGGAAAGCTG GAGCCTCACC TTGTACTTGA CCAGCTACGC 
TGTAATGGTG TCCTGGAGGG AATCCGTATT TGCAGACAGG GCTTCCCAAA TCGAATTGTA 
TTCCAAGAGT TTAGGCAAAG GTATGAGATT CTGACCCCAA ATGCCATTCC CAAAGGATTC 
ATGGATGGCA AGCAAGCTTG TGCAATAATG ATCAGAGCTT TGGAGCTTGA CCCTAACCTG 
TACCGCATAG GACAAAGTAA AATCTTCTTC CGAGCTGGAG TACTGGCACA CTTGGAAGAG 
GAAAGAGACC TAAAGATCAC TGACATTATT GTATTTTTCC AAGCTGCTGC TCGGGGTTAC 
CTGGCCAGGA GGGCATTTTA TAAGAAGCAG CAGCAGATGA GTGCTCTAAA AGTGGTGCAG 
CGGAATTGCG CCGCCTACCT CAAGCTCAGA CACTGGCAGT GGTGGAGGCT CTTCACCAAG 
GTAAAGCCAT TACTGCAAGT AACCCGTCAG GATGAGGTCA TGCAAGCAAA GGTTGTAGAA 
CTGCAAAAGG TGAAAGACAC CCAAGTAAAG ACTGAATCAG AGCTGAAGGA AATGGCTAAC 
AAGTACCAGC AGTTGTTTGA AGAGAAGAGC ATCTTGGCTG AGCAGTTACA AGCAGAAACA 
GAGCTTTTTG CCGAAGCAGA AGAAATGCGC GCCAGACTTG CATCCAAAAA GCAGGAATTG 
GAAGAGATTT TGCATGACTT GGAGGCTCGT GTGGAAGAGG AGGAAGAACG TACCTTGCAG 
CTACAAAACG AGAAGAAAAA GATGCATCAA CATATACAGG ATTTGGAAGA ACAGCTGGAG 
GAAGAAGAAG GAGCTCGACA GAAACTACAG CTAGAAAAAG TAACCACAGA GTCTAGGCTG 
AAGAAAATGG AAGAAGATAT ACTCTTACTG GAAGACCAGA ATGCCAAACT GGCCAAGGAG 
AGGAAACTGC TGGATGACCG CATTGGTGAA TTCACTTCAA CTATGGCTGA AGAAGAGGAG 
AAAGTGAAGA GCTTGAATAA ATTGCGTAAC AAATACGAAG CCGTCATTGC TGACCTGGAA 
GATCGTCTTA AAAAGGAAGA AAAGGGAAGG CAAGAGATGG AGAAGATGAA GAGGAAGCTG 
GATGGTGAAA CCACTGATTT ACAGGATCAG CTGCTAGAGC TGCAGCAGCA GATAGAGGAA 
CTAAAGCAAC AGCTGGCTCG TAAGGAAGAA GAACTCCAAG CTGCTCTTGC AAGAGTTGAT 
GATGAAGTAG GACAAAAGAA CAATTTGCTC AAACAACTTC GAGACTTGCA GTCACAGCTC 
GCTGAGTTAC ATGAAGATCT AGAGTCAGAG AAGGCTGCAC GAGCAAAGGC AGAAAAGCAG 
CGTCGGGACC TTGGTGAGGA GTTGGAGGCC CTGAAAACAG AGCTGGAAGA CACTCTCGAC 
TCCACTGCTG CACAGCAAGA GCTCAGAGCC AAAAGGGAAC AAGAGGTGAC AGACCTGAAG 
AAAACAATAG AGGAAGACGT AAAAGTTCGA GATGCCCAAG TAACAGAAAT GCGCCAGCGA 
CACAACCAAG TGGTGGAAGA GATCTCTGAA CAGCTGGAGC AGGCACGAAG GTTCAAGGGG 
AATCTGGAGA AGGTAAAGCA GACCCTGGAG AGTGAGAACA CTGACCTCAT TAAGGAAGTC 
AAGAATCTTC AAGCAGCTAA ACAGGATTCA GAACAACGCA GGAAGAAGCT GGAGCAGCAG 
GTGTCGGAAT TTCAGATCCG CACCAACGAA AGTGAAAAGG TCAAGTTCGA ACTGGCGGAG 
AAACTGCAGA AACTGCAGGC TGAATTAGAT GGTGTATCGG GCGCCCTTGG ATCAACAGAA 
GGCAAGTCAA TCAAACTAAC CAAAGACTTG TCAACAGTGC AGTCCCAACT GCAGGATACC 
CAGGAGTTGC TTCAGGAGGA AACAAGACAA AAGTTGAATT TTAGCTCTCG AGTTAGGCAG 
CTGGAGGAGG AAAAGAATAA CCTTATGGAG AACCTAGAGG AAGAGGAATC TGCTAAGGCC 
CAGCTAAGCC GCCAGTTACA GGCTTTGCAA CAACAGCTTC TCGAGTCTAA GAAGAGGATG 
GAGGACCAGG GTGGTATGGT GGAGGCTATG GAGGAAGCCA AGAAGAAATC ATATAAAGAA 
TTAGAGTTCC TCCAGCAACG CTTTGACGAG AAACATCAAA TAAACGATAA GCTAGAGAAG 
ACAAGAAACC GCTTACAGCA GGAGCTGGAT GACTTGATGG TGGATTTGGA CCATCAACGG 
CAAATAGTCT CCAATCTGGA GAAAAAGCAG AAGAAGTTTG ACCAGATGCT AGCTGAAGAG 
AAAAACATCT CTGCACGTTA TGGAGAAGAG CGAGATCGTG CTGAGGCCGA GGCTCGTGAG 
AAGGAAACAA AGGCCCTGTC TCTAAGTCGG GCCCTGGAAG AGGCCATTGA CCTAAAAGAT 
GAGCTGGACA GGCAGAACAA GCAATTAAGA GCAGAGATGG ATGATCTAGT TAGCTCCAAA 
GATGATGTTG GCAAGAATGT TCATGAGCTG GAAAGGTCTA AACGGGCCTT GGAACAGCAA 
GTTCAGGAAA TGAAGACCCA GATTGAGGAA CTGGAGGATG AGCTACAGGC CATTGAAGAT 
GGCAAACTTC GCTTGGAGGT CAATATGCAA GCAATGAAAG CTCAGTTTGA AAGAGACCTC 
CAAAACCGAG ATGATTCCAA TGATGAAAAG AAGAAGCTGC TCTTCAAACA GGTACGAGAG 
ATGGAAGTGG AGTTGGAAGA GGAAAGGAAA CAGAAATCCC AGATTCTTGC TGCCAAGAAA 
AAGCTTGAAA TGGATCTACA GGATATGGAA AGCCAGATGG ACTCTGCTAA CAAAGGTCGG 
GATGAAGCTG TTAAACAGCT GAAAAAACTA CAGCTCCAGT TCAAAGAAGT GTGGCGTGAA 
GTGGAGGAGA CCAGGGCAGC ACGAGATGAG ATCTTTGTTC AGTCTAGGGA TAATGAGAAG 
AAACTGAAGA GCCTGGAGGC TGAACTGCTT CAACTACAAG AGGACCTGGC AGCCGCCGAA 
CGTGCAAAGA GACAAGCTCA GCAGGAGAGA GACGACCTGG CAGATGAGCT GTCCAATGGA 
GTCAGTGGCA AATCGGCCCT GCTGGATGAG AAGAGAGCCC TGGAGATGCG TATCTCTCAG 
CTGGAAGAGG AGCTGGATGA AGAGCAGAGT AATACAGAGC TTATTAATGA CAGATACAGG 
AAATTAACTT TGCAGGTGGA GACAATAACC ACTGAGCTCT CAGCCGAGCG AAGCTTTTCT 
CAGAAGGCAG AGAATGCCCG TCAACAAATG GAGAGGCAGA ATAAAGAACT TAAGGTGAAA 
CTGAATGAGA TGGATTCCAC CATGAGGTCG AAGTACAAGA TCACCATCGC CTCTCTTGAG 
GCCAAGATTA GCCAGCTGGA AGAGCAGATG GAGCAGGAGT CTAAGGAACG TATCATAGCC 
AATAAGCTGG TCCGACGTGC TGAAAAGCGG CTTAAGGAGG TGCTGCTGCA GGTGGAAGAG 
GAAAGACGGA ATGCAGATCA GTTCAAGGAG CAGCTGGAGA AGGCAAATAT ACGCATGAAG 
CAGCTGAAGC GCCAATTGGA GGAGGCCGAG GAAGAGGCTT CACGCGCCAA CTCCAACCGC 
CGCCGGCTGC AGAGAGAGCT GGAAGATGTG ACTGAATCAG CAGAGTCCAT GAACCGAGAA 
GTGACCACCC TGAGAAGCAG GCTTAGCAAA TTAGAGCGTC AGCAACGCAA GCGGGCACCT 
ATACAGTTCA CCACAAGAAC TATCCGCCAG GTGTATCAAC TGGAAGCAGT TTCCGACGAG 
GAACCCGAAT CCCACAGCGG AGAACCATCT GCCAACCATC AGCAGCAGCA GCCTCCCCAG 
GCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493774 ( XM_018095487.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950976.2 Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X6, mRNA.

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CloneID	OXa61972
Clone ID Related Accession (Same CDS sequence)	XM_018095487.2
Accession Version	XM_018095487.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGCT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG CTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGATGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGGCG GGCACCTATA 
CAGTTCACCA CAAGAACTAT CCGCCAGGTG TATCAACTGG AAGCAGTTTC CGACGAGGAA 
CCCGAATCCC ACAGCGGAGA ACCATCTGCC AACCATCAGC AGCAGCAGCC TCCCCAGGCA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950976.2
CDS	181..6126
Translation	MSRNSYRDDAERYLFVDRNPTSVTATQADWTAKRQVWVPSEKHGFEAASIKEERGEEVIVELAENGKRVPVAKDDIQKMNPPKFTKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYTEQIVEMYRGKKRHEIPPHIYAISETAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKEHTAPSSSNTFYGELEHQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAIRQAKDERTFHVFYQLLAGAGEHMKTDLLLEGFNQYRFLSNGNLPIPGQQDREIFQETMESMKIMGFNHEEIMSMLKMVSAVLQFGNIVFRKERNTDQASMPDNTAAQKLCHLLGLNVTEFSRAILMPRIKVGRDYVQKAQTKEQADFAVEALAKALYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLSLLDEECWFPKATDKSFVEKVIQELGNHPKFQKPRQLRDKADLCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSTDKYTAELWKDVDRIVGLDQVSGMGEMSFGSSYKTKKGMFRTVGQLYKESLSKLMSTLRNTNPNFVRCIIPNHEKKAGKLEPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACAIMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIVFFQAAARGYLARRAFYKKQQQMSALKVVQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVMQAKVVELQKVKDTQVKTESELKEMANKYQQLFEEKSILAEQLQAETELFAEAEEMRARLASKKQELEEILHDLEARVEEEEERTLQLQNEKKKMHQHIQDLEEQLEEEEGARQKLQLEKVTTESRLKKMEEDILLLEDQNAKLAKERKLLDDRIGEFTSTMAEEEEKVKSLNKLRNKYEAVIADLEDRLKKEEKGRQEMEKMKRKLDGETTDLQDQLLELQQQIEELKQQLARKEEELQAALARVDDEVGQKNNLLKQLRDLQSQLAELHEDLESEKAARAKAEKQRRDLGEELEALKTELEDTLDSTAAQQELRAKREQEVTDLKKTIEEDVKVRDAQVTEMRQRHNQVVEEISEQLEQARRFKGNLEKVKQTLESENTDLIKEVKNLQAAKQDSEQRRKKLEQQVSEFQIRTNESEKVKFELAEKLQKLQAELDGVSGALGSTEGKSIKLTKDLSTVQSQLQDTQELLQEETRQKLNFSSRVRQLEEEKNNLMENLEEEESAKAQLSRQLQALQQQLLESKKRMEDQGGMVEAMEEAKKKSYKELEFLQQRFDEKHQINDKLEKTRNRLQQELDDLMVDLDHQRQIVSNLEKKQKKFDQMLAEEKNISARYGEERDRAEAEAREKETKALSLSRALEEAIDLKDELDRQNKQLRAEMDDLVSSKDDVGKNVHELERSKRALEQQVQEMKTQIEELEDELQAIEDGKLRLEVNMQAMKAQFERDLQNRDDSNDEKKKLLFKQVREMEVELEEERKQKSQILAAKKKLEMDLQDMESQMDSANKGRDEAVKQLKKLQLQFKEVWREVEETRAARDEIFVQSRDNEKKLKSLEAELLQLQEDLAAAERAKRQAQQERDDLADELSNGVSGKSALLDEKRALEMRISQLEEELDEEQSNTELINDRYRKLTLQVETITTELSAERSFSQKAENARQQMERQNKELKVKLNEMDSTMRSKYKITIASLEAKISQLEEQMEQESKERIIANKLVRRAEKRLKEVLLQVEEERRNADQFKEQLEKANIRMKQLKRQLEEAEEEASRANSNRRRLQRELEDVTESAESMNREVTTLRSRLRRAPIQFTTRTIRQVYQLEAVSDEEPESHSGEPSANHQQQQPPQAE

Target ORF information:

RefSeq Version	XM_018095487.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095487.2

ORF Insert Sequence:

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGCT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG CTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGATGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGGCG GGCACCTATA 
CAGTTCACCA CAAGAACTAT CCGCCAGGTG TATCAACTGG AAGCAGTTTC CGACGAGGAA 
CCCGAATCCC ACAGCGGAGA ACCATCTGCC AACCATCAGC AGCAGCAGCC TCCCCAGGCA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493774 ( XM_031905589.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761449.1 Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X7, mRNA.

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CloneID	OXa61973
Clone ID Related Accession (Same CDS sequence)	XM_031905589.1
Accession Version	XM_031905589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-10 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGGGAGAGC TAGAGCACCA GCTCCTGCAG 
GCCAACCCGA TATTGGAAGC TTTTGGCAAT GCCAAGACTG TAAAAAATGA CAACTCTTCT 
CGATTTGGTA AATTTATCCG CATTAATTTT GATGTTGCTG GTTACATCGT TGGAGCCAAC 
ATTGAGACTT ATTTGTTGGA GAAGTCTCGT GCTATTCGAC AAGCTAAAGA CGAAAGAACA 
TTCCATGTTT TTTACCAGCT TCTAGCAGGA GCAGGGGAGC ACATGAAAAC CGACTTGCTT 
CTGGAGGGCT TCAATCAGTA CCGTTTCTTG TCTAATGGAA ACCTGCCCAT TCCTGGGCAG 
CAGGACAGGG AGATCTTCCA AGAGACAATG GAGTCCATGA AAATCATGGG ATTTAACCAT 
GAAGAAATCA TGTCAATGCT GAAAATGGTG TCAGCGGTTC TGCAGTTTGG CAATATAGTT 
TTCCGGAAAG AAAGGAACAC AGATCAAGCA TCAATGCCAG ATAACACAGC TGCTCAGAAA 
CTGTGCCACC TCCTGGGACT CAATGTCACA GAGTTTAGTC GTGCCATCCT GATGCCAAGG 
ATTAAAGTTG GCCGTGATTA TGTCCAGAAG GCCCAGACTA AAGAACAGGC GGACTTTGCT 
GTGGAAGCCC TGGCAAAAGC TCTATATGAA AGACTGTTCC GCTGGTTGGT TCACCGCATT 
AACAAGGCTC TGGACAGGAC CAAAAGGCAA GGCGCCTCTT TCATAGGCAT CCTGGATATC 
GCTGGCTTTG AAATCTTTGA GCTGAACTCC TTTGAACAGC TGTGCATCAA TTACACAAAT 
GAGAAGCTTC AGCAACTGTT CAACCACACA ATGTTTGTTC TGGAGCAGGA GGAATATCAG 
CGTGAGGGCA TTGAGTGGAA CTTTATTGAC TTTGGGCTTG ATCTGCAACC CTGTATTGAC 
CTCATTGAGA GACCGGCAAA CCCTCCAGGT GTCCTGTCCC TGCTGGATGA GGAGTGCTGG 
TTCCCCAAAG CCACTGATAA GAGCTTTGTT GAAAAAGTTA TCCAGGAATT GGGAAATCAC 
CCTAAATTCC AGAAACCTCG ACAATTGCGA GACAAAGCTG ATTTGTGCAT CATTCATTAT 
GCTGGGAAGG TGGATTACAA AGCAGATGAA TGGCTGATGA AGAACATGGA TCCACTGAAT 
GACAATGTGG CCACCCTGCT GCACCAAAGC ACTGACAAGT ATACCGCTGA GCTTTGGAAA 
GATGTGGATC GGATCGTGGG CCTGGACCAA GTAAGTGGAA TGGGAGAGAT GTCTTTTGGC 
TCATCATACA AAACCAAGAA GGGAATGTTT CGCACAGTGG GCCAGCTCTA CAAAGAGTCC 
CTGTCCAAGC TTATGTCTAC TCTCAGAAAC ACCAACCCCA ACTTTGTACG GTGCATTATA 
CCCAATCATG AGAAGAAGGC TGGAAAGCTG GAGCCTCACC TTGTACTTGA CCAGCTACGC 
TGTAATGGTG TCCTGGAGGG AATCCGTATT TGCAGACAGG GCTTCCCAAA TCGAATTGTA 
TTCCAAGAGT TTAGGCAAAG GTATGAGATT CTGACCCCAA ATGCCATTCC CAAAGGATTC 
ATGGATGGCA AGCAAGCTTG TGCAATAATG ATCAGAGCTT TGGAGCTTGA CCCTAACCTG 
TACCGCATAG GACAAAGTAA AATCTTCTTC CGAGCTGGAG TACTGGCACA CTTGGAAGAG 
GAAAGAGACC TAAAGATCAC TGACATTATT GTATTTTTCC AAGCTGCTGC TCGGGGTTAC 
CTGGCCAGGA GGGCATTTTA TAAGAAGCAG CAGCAGATGA GTGCTCTAAA AGTGGTGCAG 
CGGAATTGCG CCGCCTACCT CAAGCTCAGA CACTGGCAGT GGTGGAGGCT CTTCACCAAG 
GTAAAGCCAT TACTGCAAGT AACCCGTCAG GATGAGGTCA TGCAAGCAAA GGTTGTAGAA 
CTGCAAAAGG TGAAAGACAC CCAAGTAAAG ACTGAATCAG AGCTGAAGGA AATGGCTAAC 
AAGTACCAGC AGTTGTTTGA AGAGAAGAGC ATCTTGGCTG AGCAGTTACA AGCAGAAACA 
GAGCTTTTTG CCGAAGCAGA AGAAATGCGC GCCAGACTTG CATCCAAAAA GCAGGAATTG 
GAAGAGATTT TGCATGACTT GGAGGCTCGT GTGGAAGAGG AGGAAGAACG TACCTTGCAG 
CTACAAAACG AGAAGAAAAA GATGCATCAA CATATACAGG ATTTGGAAGA ACAGCTGGAG 
GAAGAAGAAG GAGCTCGACA GAAACTACAG CTAGAAAAAG TAACCACAGA GTCTAGGCTG 
AAGAAAATGG AAGAAGATAT ACTCTTACTG GAAGACCAGA ATGCCAAACT GGCCAAGGAG 
AGGAAACTGC TGGATGACCG CATTGGTGAA TTCACTTCAA CTATGGCTGA AGAAGAGGAG 
AAAGTGAAGA GCTTGAATAA ATTGCGTAAC AAATACGAAG CCGTCATTGC TGACCTGGAA 
GATCGTCTTA AAAAGGAAGA AAAGGGAAGG CAAGAGATGG AGAAGATGAA GAGGAAGCTG 
GATGGTGAAA CCACTGATTT ACAGGATCAG CTGCTAGAGC TGCAGCAGCA GATAGAGGAA 
CTAAAGCAAC AGCTGGCTCG TAAGGAAGAA GAACTCCAAG CTGCTCTTGC AAGAGTTGAT 
GATGAAGTAG GACAAAAGAA CAATTTGCTC AAACAACTTC GAGACTTGCA GTCACAGCTC 
GCTGAGTTAC ATGAAGATCT AGAGTCAGAG AAGGCTGCAC GAGCAAAGGC AGAAAAGCAG 
CGTCGGGACC TTGGTGAGGA GTTGGAGGCC CTGAAAACAG AGCTGGAAGA CACTCTCGAC 
TCCACTGCTG CACAGCAAGA GCTCAGAGCC AAAAGGGAAC AAGAGGTGAC AGACCTGAAG 
AAAACAATAG AGGAAGACGT AAAAGTTCGA GATGCCCAAG TAACAGAAAT GCGCCAGCGA 
CACAACCAAG TGGTGGAAGA GATCTCTGAA CAGCTGGAGC AGGCACGAAG GTTCAAGGGG 
AATCTGGAGA AGGTAAAGCA GACCCTGGAG AGTGAGAACA CTGACCTCAT TAAGGAAGTC 
AAGAATCTTC AAGCAGCTAA ACAGGATTCA GAACAACGCA GGAAGAAGCT GGAGCAGCAG 
GTGTCGGAAT TTCAGATCCG CACCAACGAA AGTGAAAAGG TCAAGTTCGA ACTGGCGGAG 
AAACTGCAGA AACTGCAGGC TGAATTAGAT GGTGTATCGG GCGCCCTTGG ATCAACAGAA 
GGCAAGTCAA TCAAACTAAC CAAAGACTTG TCAACAGTGC AGTCCCAACT GCAGGATACC 
CAGGAGTTGC TTCAGGAGGA AACAAGACAA AAGTTGAATT TTAGCTCTCG AGTTAGGCAG 
CTGGAGGAGG AAAAGAATAA CCTTATGGAG AACCTAGAGG AAGAGGAATC TGCTAAGGCC 
CAGCTAAGCC GCCAGTTACA GGCTTTGCAA CAACAGCTTC TCGAGTCTAA GAAGAGGATG 
GAGGACCAGG GTGGTATGGT GGAGGCTATG GAGGAAGCCA AGAAGAAATC ATATAAAGAA 
TTAGAGTTCC TCCAGCAACG CTTTGACGAG AAACATCAAA TAAACGATAA GCTAGAGAAG 
ACAAGAAACC GCTTACAGCA GGAGCTGGAT GACTTGATGG TGGATTTGGA CCATCAACGG 
CAAATAGTCT CCAATCTGGA GAAAAAGCAG AAGAAGTTTG ACCAGATGCT AGCTGAAGAG 
AAAAACATCT CTGCACGTTA TGGAGAAGAG CGAGATCGTG CTGAGGCCGA GGCTCGTGAG 
AAGGAAACAA AGGCCCTGTC TCTAAGTCGG GCCCTGGAAG AGGCCATTGA CCTAAAAGAT 
GAGCTGGACA GGCAGAACAA GCAATTAAGA GCAGAGATGG ATGATCTAGT TAGCTCCAAA 
GATGATGTTG GCAAGAATGT TCATGAGCTG GAAAGGTCTA AACGGGCCTT GGAACAGCAA 
GTTCAGGAAA TGAAGACCCA GATTGAGGAA CTGGAGGATG AGCTACAGGC CATTGAAGAT 
GGCAAACTTC GCTTGGAGGT CAATATGCAA GCAATGAAAG CTCAGTTTGA AAGAGACCTC 
CAAAACCGAG ATGATTCCAA TGATGAAAAG AAGAAGCTGC TCTTCAAACA GGTACGAGAG 
ATGGAAGTGG AGTTGGAAGA GGAAAGGAAA CAGAAATCCC AGATTCTTGC TGCCAAGAAA 
AAGCTTGAAA TGGATCTACA GGATATGGAA AGCCAGATGG ACTCTGCTAA CAAAGGTCGG 
GATGAAGCTG TTAAACAGCT GAAAAAACTA CAGCTCCAGT TCAAAGAAGT GTGGCGTGAA 
GTGGAGGAGA CCAGGGCAGC ACGAGATGAG ATCTTTGTTC AGTCTAGGGA TAATGAGAAG 
AAACTGAAGA GCCTGGAGGC TGAACTGCTT CAACTACAAG AGGACCTGGC AGCCGCCGAA 
CGTGCAAAGA GACAAGCTCA GCAGGAGAGA GACGACCTGG CAGATGAGCT GTCCAATGGA 
GTCAGTGGCA AATCGGCCCT GCTGGATGAG AAGAGAGCCC TGGAGATGCG TATCTCTCAG 
CTGGAAGAGG AGCTGGATGA AGAGCAGAGT AATACAGAGC TTATTAATGA CAGATACAGG 
AAATTAACTT TGCAGGTGGA GACAATAACC ACTGAGCTCT CAGCCGAGCG AAGCTTTTCT 
CAGAAGGCAG AGAATGCCCG TCAACAAATG GAGAGGCAGA ATAAAGAACT TAAGGTGAAA 
CTGAATGAGA TGGATTCCAC CATGAGGTCG AAGTACAAGA TCACCATCGC CTCTCTTGAG 
GCCAAGATTA GCCAGCTGGA AGAGCAGATG GAGCAGGAGT CTAAGGAACG TATCATAGCC 
AATAAGCTGG TCCGACGTGC TGAAAAGCGG CTTAAGGAGG TGCTGCTGCA GGTGGAAGAG 
GAAAGACGGA ATGCAGATCA GTTCAAGGAG CAGCTGGAGA AGGCAAATAT ACGCATGAAG 
CAGCTGAAGC GCCAATTGGA GGAGGCCGAG GAAGAGGCTT CACGCGCCAA CTCCAACCGC 
CGCCGGCTGC AGAGAGAGCT GGAAGATGTG ACTGAATCAG CAGAGTCCAT GAACCGAGAA 
GTGACCACCC TGAGAAGCAG GCTTAGGCGG GCACCTATAC AGTTCACCAC AAGAACTATC 
CGCCAGGTGT ATCAACTGGA AGCAGTTTCC GACGAGGAAC CCGAATCCCA CAGCGGAGAA 
CCATCTGCCA ACCATCAGCA GCAGCAGCCT CCCCAGGCAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761449.1
CDS	181..6105
Translation	MSRNSYRDDAERYLFVDRNPTSVTATQADWTAKRQVWVPSEKHGFEAASIKEERGEEVIVELAENGKRVPVAKDDIQKMNPPKFTKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYTEQIVEMYRGKKRHEIPPHIYAISETAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKEHTAPGELEHQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAIRQAKDERTFHVFYQLLAGAGEHMKTDLLLEGFNQYRFLSNGNLPIPGQQDREIFQETMESMKIMGFNHEEIMSMLKMVSAVLQFGNIVFRKERNTDQASMPDNTAAQKLCHLLGLNVTEFSRAILMPRIKVGRDYVQKAQTKEQADFAVEALAKALYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLSLLDEECWFPKATDKSFVEKVIQELGNHPKFQKPRQLRDKADLCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSTDKYTAELWKDVDRIVGLDQVSGMGEMSFGSSYKTKKGMFRTVGQLYKESLSKLMSTLRNTNPNFVRCIIPNHEKKAGKLEPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACAIMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIVFFQAAARGYLARRAFYKKQQQMSALKVVQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVMQAKVVELQKVKDTQVKTESELKEMANKYQQLFEEKSILAEQLQAETELFAEAEEMRARLASKKQELEEILHDLEARVEEEEERTLQLQNEKKKMHQHIQDLEEQLEEEEGARQKLQLEKVTTESRLKKMEEDILLLEDQNAKLAKERKLLDDRIGEFTSTMAEEEEKVKSLNKLRNKYEAVIADLEDRLKKEEKGRQEMEKMKRKLDGETTDLQDQLLELQQQIEELKQQLARKEEELQAALARVDDEVGQKNNLLKQLRDLQSQLAELHEDLESEKAARAKAEKQRRDLGEELEALKTELEDTLDSTAAQQELRAKREQEVTDLKKTIEEDVKVRDAQVTEMRQRHNQVVEEISEQLEQARRFKGNLEKVKQTLESENTDLIKEVKNLQAAKQDSEQRRKKLEQQVSEFQIRTNESEKVKFELAEKLQKLQAELDGVSGALGSTEGKSIKLTKDLSTVQSQLQDTQELLQEETRQKLNFSSRVRQLEEEKNNLMENLEEEESAKAQLSRQLQALQQQLLESKKRMEDQGGMVEAMEEAKKKSYKELEFLQQRFDEKHQINDKLEKTRNRLQQELDDLMVDLDHQRQIVSNLEKKQKKFDQMLAEEKNISARYGEERDRAEAEAREKETKALSLSRALEEAIDLKDELDRQNKQLRAEMDDLVSSKDDVGKNVHELERSKRALEQQVQEMKTQIEELEDELQAIEDGKLRLEVNMQAMKAQFERDLQNRDDSNDEKKKLLFKQVREMEVELEEERKQKSQILAAKKKLEMDLQDMESQMDSANKGRDEAVKQLKKLQLQFKEVWREVEETRAARDEIFVQSRDNEKKLKSLEAELLQLQEDLAAAERAKRQAQQERDDLADELSNGVSGKSALLDEKRALEMRISQLEEELDEEQSNTELINDRYRKLTLQVETITTELSAERSFSQKAENARQQMERQNKELKVKLNEMDSTMRSKYKITIASLEAKISQLEEQMEQESKERIIANKLVRRAEKRLKEVLLQVEEERRNADQFKEQLEKANIRMKQLKRQLEEAEEEASRANSNRRRLQRELEDVTESAESMNREVTTLRSRLRRAPIQFTTRTIRQVYQLEAVSDEEPESHSGEPSANHQQQQPPQAE

Target ORF information:

RefSeq Version	XM_031905589.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-10 (LOC100493774), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905589.1

ORF Insert Sequence:

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGGGAGAGC TAGAGCACCA GCTCCTGCAG 
GCCAACCCGA TATTGGAAGC TTTTGGCAAT GCCAAGACTG TAAAAAATGA CAACTCTTCT 
CGATTTGGTA AATTTATCCG CATTAATTTT GATGTTGCTG GTTACATCGT TGGAGCCAAC 
ATTGAGACTT ATTTGTTGGA GAAGTCTCGT GCTATTCGAC AAGCTAAAGA CGAAAGAACA 
TTCCATGTTT TTTACCAGCT TCTAGCAGGA GCAGGGGAGC ACATGAAAAC CGACTTGCTT 
CTGGAGGGCT TCAATCAGTA CCGTTTCTTG TCTAATGGAA ACCTGCCCAT TCCTGGGCAG 
CAGGACAGGG AGATCTTCCA AGAGACAATG GAGTCCATGA AAATCATGGG ATTTAACCAT 
GAAGAAATCA TGTCAATGCT GAAAATGGTG TCAGCGGTTC TGCAGTTTGG CAATATAGTT 
TTCCGGAAAG AAAGGAACAC AGATCAAGCA TCAATGCCAG ATAACACAGC TGCTCAGAAA 
CTGTGCCACC TCCTGGGACT CAATGTCACA GAGTTTAGTC GTGCCATCCT GATGCCAAGG 
ATTAAAGTTG GCCGTGATTA TGTCCAGAAG GCCCAGACTA AAGAACAGGC GGACTTTGCT 
GTGGAAGCCC TGGCAAAAGC TCTATATGAA AGACTGTTCC GCTGGTTGGT TCACCGCATT 
AACAAGGCTC TGGACAGGAC CAAAAGGCAA GGCGCCTCTT TCATAGGCAT CCTGGATATC 
GCTGGCTTTG AAATCTTTGA GCTGAACTCC TTTGAACAGC TGTGCATCAA TTACACAAAT 
GAGAAGCTTC AGCAACTGTT CAACCACACA ATGTTTGTTC TGGAGCAGGA GGAATATCAG 
CGTGAGGGCA TTGAGTGGAA CTTTATTGAC TTTGGGCTTG ATCTGCAACC CTGTATTGAC 
CTCATTGAGA GACCGGCAAA CCCTCCAGGT GTCCTGTCCC TGCTGGATGA GGAGTGCTGG 
TTCCCCAAAG CCACTGATAA GAGCTTTGTT GAAAAAGTTA TCCAGGAATT GGGAAATCAC 
CCTAAATTCC AGAAACCTCG ACAATTGCGA GACAAAGCTG ATTTGTGCAT CATTCATTAT 
GCTGGGAAGG TGGATTACAA AGCAGATGAA TGGCTGATGA AGAACATGGA TCCACTGAAT 
GACAATGTGG CCACCCTGCT GCACCAAAGC ACTGACAAGT ATACCGCTGA GCTTTGGAAA 
GATGTGGATC GGATCGTGGG CCTGGACCAA GTAAGTGGAA TGGGAGAGAT GTCTTTTGGC 
TCATCATACA AAACCAAGAA GGGAATGTTT CGCACAGTGG GCCAGCTCTA CAAAGAGTCC 
CTGTCCAAGC TTATGTCTAC TCTCAGAAAC ACCAACCCCA ACTTTGTACG GTGCATTATA 
CCCAATCATG AGAAGAAGGC TGGAAAGCTG GAGCCTCACC TTGTACTTGA CCAGCTACGC 
TGTAATGGTG TCCTGGAGGG AATCCGTATT TGCAGACAGG GCTTCCCAAA TCGAATTGTA 
TTCCAAGAGT TTAGGCAAAG GTATGAGATT CTGACCCCAA ATGCCATTCC CAAAGGATTC 
ATGGATGGCA AGCAAGCTTG TGCAATAATG ATCAGAGCTT TGGAGCTTGA CCCTAACCTG 
TACCGCATAG GACAAAGTAA AATCTTCTTC CGAGCTGGAG TACTGGCACA CTTGGAAGAG 
GAAAGAGACC TAAAGATCAC TGACATTATT GTATTTTTCC AAGCTGCTGC TCGGGGTTAC 
CTGGCCAGGA GGGCATTTTA TAAGAAGCAG CAGCAGATGA GTGCTCTAAA AGTGGTGCAG 
CGGAATTGCG CCGCCTACCT CAAGCTCAGA CACTGGCAGT GGTGGAGGCT CTTCACCAAG 
GTAAAGCCAT TACTGCAAGT AACCCGTCAG GATGAGGTCA TGCAAGCAAA GGTTGTAGAA 
CTGCAAAAGG TGAAAGACAC CCAAGTAAAG ACTGAATCAG AGCTGAAGGA AATGGCTAAC 
AAGTACCAGC AGTTGTTTGA AGAGAAGAGC ATCTTGGCTG AGCAGTTACA AGCAGAAACA 
GAGCTTTTTG CCGAAGCAGA AGAAATGCGC GCCAGACTTG CATCCAAAAA GCAGGAATTG 
GAAGAGATTT TGCATGACTT GGAGGCTCGT GTGGAAGAGG AGGAAGAACG TACCTTGCAG 
CTACAAAACG AGAAGAAAAA GATGCATCAA CATATACAGG ATTTGGAAGA ACAGCTGGAG 
GAAGAAGAAG GAGCTCGACA GAAACTACAG CTAGAAAAAG TAACCACAGA GTCTAGGCTG 
AAGAAAATGG AAGAAGATAT ACTCTTACTG GAAGACCAGA ATGCCAAACT GGCCAAGGAG 
AGGAAACTGC TGGATGACCG CATTGGTGAA TTCACTTCAA CTATGGCTGA AGAAGAGGAG 
AAAGTGAAGA GCTTGAATAA ATTGCGTAAC AAATACGAAG CCGTCATTGC TGACCTGGAA 
GATCGTCTTA AAAAGGAAGA AAAGGGAAGG CAAGAGATGG AGAAGATGAA GAGGAAGCTG 
GATGGTGAAA CCACTGATTT ACAGGATCAG CTGCTAGAGC TGCAGCAGCA GATAGAGGAA 
CTAAAGCAAC AGCTGGCTCG TAAGGAAGAA GAACTCCAAG CTGCTCTTGC AAGAGTTGAT 
GATGAAGTAG GACAAAAGAA CAATTTGCTC AAACAACTTC GAGACTTGCA GTCACAGCTC 
GCTGAGTTAC ATGAAGATCT AGAGTCAGAG AAGGCTGCAC GAGCAAAGGC AGAAAAGCAG 
CGTCGGGACC TTGGTGAGGA GTTGGAGGCC CTGAAAACAG AGCTGGAAGA CACTCTCGAC 
TCCACTGCTG CACAGCAAGA GCTCAGAGCC AAAAGGGAAC AAGAGGTGAC AGACCTGAAG 
AAAACAATAG AGGAAGACGT AAAAGTTCGA GATGCCCAAG TAACAGAAAT GCGCCAGCGA 
CACAACCAAG TGGTGGAAGA GATCTCTGAA CAGCTGGAGC AGGCACGAAG GTTCAAGGGG 
AATCTGGAGA AGGTAAAGCA GACCCTGGAG AGTGAGAACA CTGACCTCAT TAAGGAAGTC 
AAGAATCTTC AAGCAGCTAA ACAGGATTCA GAACAACGCA GGAAGAAGCT GGAGCAGCAG 
GTGTCGGAAT TTCAGATCCG CACCAACGAA AGTGAAAAGG TCAAGTTCGA ACTGGCGGAG 
AAACTGCAGA AACTGCAGGC TGAATTAGAT GGTGTATCGG GCGCCCTTGG ATCAACAGAA 
GGCAAGTCAA TCAAACTAAC CAAAGACTTG TCAACAGTGC AGTCCCAACT GCAGGATACC 
CAGGAGTTGC TTCAGGAGGA AACAAGACAA AAGTTGAATT TTAGCTCTCG AGTTAGGCAG 
CTGGAGGAGG AAAAGAATAA CCTTATGGAG AACCTAGAGG AAGAGGAATC TGCTAAGGCC 
CAGCTAAGCC GCCAGTTACA GGCTTTGCAA CAACAGCTTC TCGAGTCTAA GAAGAGGATG 
GAGGACCAGG GTGGTATGGT GGAGGCTATG GAGGAAGCCA AGAAGAAATC ATATAAAGAA 
TTAGAGTTCC TCCAGCAACG CTTTGACGAG AAACATCAAA TAAACGATAA GCTAGAGAAG 
ACAAGAAACC GCTTACAGCA GGAGCTGGAT GACTTGATGG TGGATTTGGA CCATCAACGG 
CAAATAGTCT CCAATCTGGA GAAAAAGCAG AAGAAGTTTG ACCAGATGCT AGCTGAAGAG 
AAAAACATCT CTGCACGTTA TGGAGAAGAG CGAGATCGTG CTGAGGCCGA GGCTCGTGAG 
AAGGAAACAA AGGCCCTGTC TCTAAGTCGG GCCCTGGAAG AGGCCATTGA CCTAAAAGAT 
GAGCTGGACA GGCAGAACAA GCAATTAAGA GCAGAGATGG ATGATCTAGT TAGCTCCAAA 
GATGATGTTG GCAAGAATGT TCATGAGCTG GAAAGGTCTA AACGGGCCTT GGAACAGCAA 
GTTCAGGAAA TGAAGACCCA GATTGAGGAA CTGGAGGATG AGCTACAGGC CATTGAAGAT 
GGCAAACTTC GCTTGGAGGT CAATATGCAA GCAATGAAAG CTCAGTTTGA AAGAGACCTC 
CAAAACCGAG ATGATTCCAA TGATGAAAAG AAGAAGCTGC TCTTCAAACA GGTACGAGAG 
ATGGAAGTGG AGTTGGAAGA GGAAAGGAAA CAGAAATCCC AGATTCTTGC TGCCAAGAAA 
AAGCTTGAAA TGGATCTACA GGATATGGAA AGCCAGATGG ACTCTGCTAA CAAAGGTCGG 
GATGAAGCTG TTAAACAGCT GAAAAAACTA CAGCTCCAGT TCAAAGAAGT GTGGCGTGAA 
GTGGAGGAGA CCAGGGCAGC ACGAGATGAG ATCTTTGTTC AGTCTAGGGA TAATGAGAAG 
AAACTGAAGA GCCTGGAGGC TGAACTGCTT CAACTACAAG AGGACCTGGC AGCCGCCGAA 
CGTGCAAAGA GACAAGCTCA GCAGGAGAGA GACGACCTGG CAGATGAGCT GTCCAATGGA 
GTCAGTGGCA AATCGGCCCT GCTGGATGAG AAGAGAGCCC TGGAGATGCG TATCTCTCAG 
CTGGAAGAGG AGCTGGATGA AGAGCAGAGT AATACAGAGC TTATTAATGA CAGATACAGG 
AAATTAACTT TGCAGGTGGA GACAATAACC ACTGAGCTCT CAGCCGAGCG AAGCTTTTCT 
CAGAAGGCAG AGAATGCCCG TCAACAAATG GAGAGGCAGA ATAAAGAACT TAAGGTGAAA 
CTGAATGAGA TGGATTCCAC CATGAGGTCG AAGTACAAGA TCACCATCGC CTCTCTTGAG 
GCCAAGATTA GCCAGCTGGA AGAGCAGATG GAGCAGGAGT CTAAGGAACG TATCATAGCC 
AATAAGCTGG TCCGACGTGC TGAAAAGCGG CTTAAGGAGG TGCTGCTGCA GGTGGAAGAG 
GAAAGACGGA ATGCAGATCA GTTCAAGGAG CAGCTGGAGA AGGCAAATAT ACGCATGAAG 
CAGCTGAAGC GCCAATTGGA GGAGGCCGAG GAAGAGGCTT CACGCGCCAA CTCCAACCGC 
CGCCGGCTGC AGAGAGAGCT GGAAGATGTG ACTGAATCAG CAGAGTCCAT GAACCGAGAA 
GTGACCACCC TGAGAAGCAG GCTTAGGCGG GCACCTATAC AGTTCACCAC AAGAACTATC 
CGCCAGGTGT ATCAACTGGA AGCAGTTTCC GACGAGGAAC CCGAATCCCA CAGCGGAGAA 
CCATCTGCCA ACCATCAGCA GCAGCAGCCT CCCCAGGCAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493774 ( XM_012967418.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822872.1 Xenopus tropicalis myosin-10-like (LOC100493774), transcript variant X3, mRNA.

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CloneID	OXa13288
Clone ID Related Accession (Same CDS sequence)	XM_012967418.1 , XM_012967417.2 , XM_004916218.3
Accession Version	XM_012967418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-10-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGTT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG TTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGACGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822872.1
CDS	342..6311
Translation	MSRNSYRDDAERYLFVDRNPTSVTATQADWTAKRQVWVPSEKHGFEAASIKEERGEEVIVELAENGKRVPVAKDDIQKMNPPKFTKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYTEQIVEMYRGKKRHEIPPHIYAISETAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKEHTAPSSSNTFYGELEHQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAIRQAKDERTFHVFYQLLAGAGEHMKTDLLLEGFNQYRFLSNGNLPIPGQQDREIFQETMESMKIMGFNHEEIMSMLKMVSAVLQFGNIVFRKERNTDQASMPDNTAAQKLCHLLGLNVTEFSRAILMPRIKVGRDYVQKAQTKEQADFAVEALAKALYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLSLLDEECWFPKATDKSFVEKVIQELGNHPKFQKPRQLRDKADLCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSTDKYTAELWKDVDRIVGLDQVSGMGEMSFGSSYKTKKGMFRTVGQLYKESLSKLMSTLRNTNPNFVRCIIPNHEKKAGKLEPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACAIMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIVFFQAAARGYLARRAFYKKQQQMSALKVVQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVMQAKVVELQKVKDTQVKTESELKEMANKYQQLFEEKSILAEQLQAETELFAEAEEMRARLASKKQELEEILHDLEARVEEEEERTLQLQNEKKKMHQHIQDLEEQLEEEEGARQKLQLEKVTTESRLKKMEEDILLLEDQNAKLAKERKLLDDRIGEFTSTMAEEEEKVKSLNKLRNKYEAVIADLEDRLKKEEKGRQEMEKMKRKLDGETTDLQDQLLELQQQIEELKQQLARKEEELQAALARVDDEVGQKNNLLKQLRDLQSQLAELHEDLESEKAARAKAEKQRRDLGEELEALKTELEDTLDSTAAQQELRAKREQEVTDLKKTIEEDVKVRDAQVTEMRQRHNQVVEEISEQLEQARRFKGNLEKVKQTLESENTDLIKEVKNLQAAKQDSEQRRKKLEQQVSEFQIRTNESEKVKFELAEKLQKLQAELDGVSGALGSTEGKSIKLTKDLSTVQSQLQDTQELLQEETRQKLNFSSRVRQLEEEKNNLMENLEEEESAKAQLSRQLQALQQQLLESKKRMEDQGGMVEAMEEAKKKSYKELEFLQQRFDEKHQINDKLEKTRNRLQQELDDLMVDLDHQRQIVSNLEKKQKKFDQMLAEEKNISARYGEERDRAEAEAREKETKALSLSRALEEAIDLKDELDRQNKQLRAEMDDLVSSKDDVGKNVHELERSKRALEQQVQEMKTQIEELEDELQAIEDGKLRLEVNMQAMKAQFERDLQNRDDSNDEKKKLLFKQVREMEVELEEERKQKSQILVAKKKLEMDLQDMESQMDSANKGRDEAVKQLKKLQLQFKEVWREVEETRAARDEIFVQSRDNEKKLKSLEAELLQLQEDLAAAERAKRQAQQERDDLADELSNGVSGKSALLDEKRALEMRISQLEEELDEEQSNTELINDRYRKLTLQVETITTELSAERSFSQKAENARQQMERQNKELKVKLNEMDSTMRSKYKITIASLEAKISQLEEQMEQESKERIIANKLVRRAEKRLKEVLLQVEEERRNADQFKEQLEKANIRMKQLKRQLEEAEEEASRANSNRRRLQRELEDVTESAESMNREVTTLRSRLSKLERQQRKRAPIQFTTRTIRQVYQLEAVSDEEPESHSGEPSANHQQQQPPQAE

Target ORF information:

RefSeq Version	XM_012967418.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-10-like (LOC100493774), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967418.1

ORF Insert Sequence:

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGTT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG TTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGACGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493774 ( XM_012967417.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822871.1 Xenopus tropicalis myosin-10-like (LOC100493774), transcript variant X1, mRNA.

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CloneID	OXa13288
Clone ID Related Accession (Same CDS sequence)	XM_012967418.1 , XM_012967417.2 , XM_004916218.3
Accession Version	XM_012967417.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-10-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967417.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGTT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG TTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGACGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822871.1
CDS	180..6149
Translation	MSRNSYRDDAERYLFVDRNPTSVTATQADWTAKRQVWVPSEKHGFEAASIKEERGEEVIVELAENGKRVPVAKDDIQKMNPPKFTKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYTEQIVEMYRGKKRHEIPPHIYAISETAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKEHTAPSSSNTFYGELEHQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAIRQAKDERTFHVFYQLLAGAGEHMKTDLLLEGFNQYRFLSNGNLPIPGQQDREIFQETMESMKIMGFNHEEIMSMLKMVSAVLQFGNIVFRKERNTDQASMPDNTAAQKLCHLLGLNVTEFSRAILMPRIKVGRDYVQKAQTKEQADFAVEALAKALYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLSLLDEECWFPKATDKSFVEKVIQELGNHPKFQKPRQLRDKADLCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSTDKYTAELWKDVDRIVGLDQVSGMGEMSFGSSYKTKKGMFRTVGQLYKESLSKLMSTLRNTNPNFVRCIIPNHEKKAGKLEPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACAIMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIVFFQAAARGYLARRAFYKKQQQMSALKVVQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVMQAKVVELQKVKDTQVKTESELKEMANKYQQLFEEKSILAEQLQAETELFAEAEEMRARLASKKQELEEILHDLEARVEEEEERTLQLQNEKKKMHQHIQDLEEQLEEEEGARQKLQLEKVTTESRLKKMEEDILLLEDQNAKLAKERKLLDDRIGEFTSTMAEEEEKVKSLNKLRNKYEAVIADLEDRLKKEEKGRQEMEKMKRKLDGETTDLQDQLLELQQQIEELKQQLARKEEELQAALARVDDEVGQKNNLLKQLRDLQSQLAELHEDLESEKAARAKAEKQRRDLGEELEALKTELEDTLDSTAAQQELRAKREQEVTDLKKTIEEDVKVRDAQVTEMRQRHNQVVEEISEQLEQARRFKGNLEKVKQTLESENTDLIKEVKNLQAAKQDSEQRRKKLEQQVSEFQIRTNESEKVKFELAEKLQKLQAELDGVSGALGSTEGKSIKLTKDLSTVQSQLQDTQELLQEETRQKLNFSSRVRQLEEEKNNLMENLEEEESAKAQLSRQLQALQQQLLESKKRMEDQGGMVEAMEEAKKKSYKELEFLQQRFDEKHQINDKLEKTRNRLQQELDDLMVDLDHQRQIVSNLEKKQKKFDQMLAEEKNISARYGEERDRAEAEAREKETKALSLSRALEEAIDLKDELDRQNKQLRAEMDDLVSSKDDVGKNVHELERSKRALEQQVQEMKTQIEELEDELQAIEDGKLRLEVNMQAMKAQFERDLQNRDDSNDEKKKLLFKQVREMEVELEEERKQKSQILVAKKKLEMDLQDMESQMDSANKGRDEAVKQLKKLQLQFKEVWREVEETRAARDEIFVQSRDNEKKLKSLEAELLQLQEDLAAAERAKRQAQQERDDLADELSNGVSGKSALLDEKRALEMRISQLEEELDEEQSNTELINDRYRKLTLQVETITTELSAERSFSQKAENARQQMERQNKELKVKLNEMDSTMRSKYKITIASLEAKISQLEEQMEQESKERIIANKLVRRAEKRLKEVLLQVEEERRNADQFKEQLEKANIRMKQLKRQLEEAEEEASRANSNRRRLQRELEDVTESAESMNREVTTLRSRLSKLERQQRKRAPIQFTTRTIRQVYQLEAVSDEEPESHSGEPSANHQQQQPPQAE

Target ORF information:

RefSeq Version	XM_012967417.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-10-like (LOC100493774), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967417.2

ORF Insert Sequence:

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGTT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG TTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGACGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100493774 ( XM_004916218.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916275.1 Xenopus tropicalis myosin-10-like (LOC100493774), transcript variant X2, mRNA.

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CloneID	OXa13288
Clone ID Related Accession (Same CDS sequence)	XM_012967418.1 , XM_012967417.2 , XM_004916218.3
Accession Version	XM_004916218.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	myosin-10-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916218.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGTT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG TTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGACGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916275.1
CDS	247..6216
Translation	MSRNSYRDDAERYLFVDRNPTSVTATQADWTAKRQVWVPSEKHGFEAASIKEERGEEVIVELAENGKRVPVAKDDIQKMNPPKFTKVEDMAELTCLNEASVLHNLKDRYYSGLIYTYSGLFCVVINPYKNLPIYTEQIVEMYRGKKRHEIPPHIYAISETAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSHKGRKEHTAPSSSNTFYGELEHQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAIRQAKDERTFHVFYQLLAGAGEHMKTDLLLEGFNQYRFLSNGNLPIPGQQDREIFQETMESMKIMGFNHEEIMSMLKMVSAVLQFGNIVFRKERNTDQASMPDNTAAQKLCHLLGLNVTEFSRAILMPRIKVGRDYVQKAQTKEQADFAVEALAKALYERLFRWLVHRINKALDRTKRQGASFIGILDIAGFEIFELNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIEWNFIDFGLDLQPCIDLIERPANPPGVLSLLDEECWFPKATDKSFVEKVIQELGNHPKFQKPRQLRDKADLCIIHYAGKVDYKADEWLMKNMDPLNDNVATLLHQSTDKYTAELWKDVDRIVGLDQVSGMGEMSFGSSYKTKKGMFRTVGQLYKESLSKLMSTLRNTNPNFVRCIIPNHEKKAGKLEPHLVLDQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILTPNAIPKGFMDGKQACAIMIRALELDPNLYRIGQSKIFFRAGVLAHLEEERDLKITDIIVFFQAAARGYLARRAFYKKQQQMSALKVVQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVMQAKVVELQKVKDTQVKTESELKEMANKYQQLFEEKSILAEQLQAETELFAEAEEMRARLASKKQELEEILHDLEARVEEEEERTLQLQNEKKKMHQHIQDLEEQLEEEEGARQKLQLEKVTTESRLKKMEEDILLLEDQNAKLAKERKLLDDRIGEFTSTMAEEEEKVKSLNKLRNKYEAVIADLEDRLKKEEKGRQEMEKMKRKLDGETTDLQDQLLELQQQIEELKQQLARKEEELQAALARVDDEVGQKNNLLKQLRDLQSQLAELHEDLESEKAARAKAEKQRRDLGEELEALKTELEDTLDSTAAQQELRAKREQEVTDLKKTIEEDVKVRDAQVTEMRQRHNQVVEEISEQLEQARRFKGNLEKVKQTLESENTDLIKEVKNLQAAKQDSEQRRKKLEQQVSEFQIRTNESEKVKFELAEKLQKLQAELDGVSGALGSTEGKSIKLTKDLSTVQSQLQDTQELLQEETRQKLNFSSRVRQLEEEKNNLMENLEEEESAKAQLSRQLQALQQQLLESKKRMEDQGGMVEAMEEAKKKSYKELEFLQQRFDEKHQINDKLEKTRNRLQQELDDLMVDLDHQRQIVSNLEKKQKKFDQMLAEEKNISARYGEERDRAEAEAREKETKALSLSRALEEAIDLKDELDRQNKQLRAEMDDLVSSKDDVGKNVHELERSKRALEQQVQEMKTQIEELEDELQAIEDGKLRLEVNMQAMKAQFERDLQNRDDSNDEKKKLLFKQVREMEVELEEERKQKSQILVAKKKLEMDLQDMESQMDSANKGRDEAVKQLKKLQLQFKEVWREVEETRAARDEIFVQSRDNEKKLKSLEAELLQLQEDLAAAERAKRQAQQERDDLADELSNGVSGKSALLDEKRALEMRISQLEEELDEEQSNTELINDRYRKLTLQVETITTELSAERSFSQKAENARQQMERQNKELKVKLNEMDSTMRSKYKITIASLEAKISQLEEQMEQESKERIIANKLVRRAEKRLKEVLLQVEEERRNADQFKEQLEKANIRMKQLKRQLEEAEEEASRANSNRRRLQRELEDVTESAESMNREVTTLRSRLSKLERQQRKRAPIQFTTRTIRQVYQLEAVSDEEPESHSGEPSANHQQQQPPQAE

Target ORF information:

RefSeq Version	XM_004916218.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis myosin-10-like (LOC100493774), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916218.3

ORF Insert Sequence:

ATGTCTCGAA ACTCCTACCG AGATGATGCA GAGCGCTACC TCTTTGTTGA CCGGAACCCT 
ACGAGCGTCA CAGCCACACA AGCCGACTGG ACAGCAAAAC GCCAGGTTTG GGTACCCTCA 
GAGAAGCATG GGTTTGAAGC AGCCAGCATA AAGGAGGAGC GTGGGGAGGA GGTCATTGTG 
GAGCTGGCAG AGAATGGCAA GAGAGTGCCC GTGGCAAAGG ATGACATCCA GAAGATGAAC 
CCACCCAAAT TTACTAAGGT GGAAGATATG GCGGAGCTGA CCTGCCTGAA TGAAGCATCA 
GTTCTGCACA ATCTCAAAGA CCGATATTAC TCGGGGCTTA TTTATACTTA TTCTGGCCTC 
TTCTGTGTTG TGATTAACCC CTACAAGAAC TTGCCCATTT ACACGGAACA AATAGTGGAG 
ATGTATCGAG GGAAGAAACG GCACGAGATT CCCCCACACA TATATGCAAT ATCAGAGACT 
GCATATCGCA GCATGCTGCA AGATCGAGAG GATCAGTCCA TCCTTTGCAC TGGAGAATCA 
GGTGCCGGCA AGACAGAGAA CACAAAGAAA GTCATCCAAT ATTTGGCACA TGTGGCTTCT 
TCACATAAGG GGCGAAAGGA GCACACAGCC CCGAGCTCCA GTAATACGTT TTACGGAGAG 
CTAGAGCACC AGCTCCTGCA GGCCAACCCG ATATTGGAAG CTTTTGGCAA TGCCAAGACT 
GTAAAAAATG ACAACTCTTC TCGATTTGGT AAATTTATCC GCATTAATTT TGATGTTGCT 
GGTTACATCG TTGGAGCCAA CATTGAGACT TATTTGTTGG AGAAGTCTCG TGCTATTCGA 
CAAGCTAAAG ACGAAAGAAC ATTCCATGTT TTTTACCAGC TTCTAGCAGG AGCAGGGGAG 
CACATGAAAA CCGACTTGCT TCTGGAGGGC TTCAATCAGT ACCGTTTCTT GTCTAATGGA 
AACCTGCCCA TTCCTGGGCA GCAGGACAGG GAGATCTTCC AAGAGACAAT GGAGTCCATG 
AAAATCATGG GATTTAACCA TGAAGAAATC ATGTCAATGC TGAAAATGGT GTCAGCGGTT 
CTGCAGTTTG GCAATATAGT TTTCCGGAAA GAAAGGAACA CAGATCAAGC ATCAATGCCA 
GATAACACAG CTGCTCAGAA ACTGTGCCAC CTCCTGGGAC TCAATGTCAC AGAGTTTAGT 
CGTGCCATCC TGATGCCAAG GATTAAAGTT GGCCGTGATT ATGTCCAGAA GGCCCAGACT 
AAAGAACAGG CGGACTTTGC TGTGGAAGCC CTGGCAAAAG CTCTATATGA AAGACTGTTC 
CGCTGGTTGG TTCACCGCAT TAACAAGGCT CTGGACAGGA CCAAAAGGCA AGGCGCCTCT 
TTCATAGGCA TCCTGGATAT CGCTGGCTTT GAAATCTTTG AGCTGAACTC CTTTGAACAG 
CTGTGCATCA ATTACACAAA TGAGAAGCTT CAGCAACTGT TCAACCACAC AATGTTTGTT 
CTGGAGCAGG AGGAATATCA GCGTGAGGGC ATTGAGTGGA ACTTTATTGA CTTTGGGCTT 
GATCTGCAAC CCTGTATTGA CCTCATTGAG AGACCGGCAA ACCCTCCAGG TGTCCTGTCC 
CTGCTGGATG AGGAGTGCTG GTTCCCCAAA GCCACTGATA AGAGCTTTGT TGAAAAAGTT 
ATCCAGGAAT TGGGAAATCA CCCTAAATTC CAGAAACCTC GACAATTGCG AGACAAAGCT 
GATTTGTGCA TCATTCATTA TGCTGGGAAG GTGGATTACA AAGCAGATGA ATGGCTGATG 
AAGAACATGG ATCCACTGAA TGACAATGTG GCCACCCTGC TGCACCAAAG CACTGACAAG 
TATACCGCTG AGCTTTGGAA AGATGTGGAT CGGATCGTGG GCCTGGACCA AGTAAGTGGA 
ATGGGAGAGA TGTCTTTTGG CTCATCATAC AAAACCAAGA AGGGAATGTT TCGCACAGTG 
GGCCAGCTCT ACAAAGAGTC CCTGTCCAAG CTTATGTCTA CTCTCAGAAA CACCAACCCC 
AACTTTGTAC GGTGCATTAT ACCCAATCAT GAGAAGAAGG CTGGAAAGTT GGAGCCTCAC 
CTTGTACTTG ACCAGCTACG CTGTAATGGT GTCCTGGAGG GAATCCGTAT TTGCAGACAG 
GGCTTCCCAA ATCGAATTGT ATTCCAAGAG TTTAGGCAAA GGTATGAGAT TCTGACCCCA 
AATGCCATTC CCAAAGGATT CATGGATGGC AAGCAAGCTT GTGCAATAAT GATCAGAGCT 
TTGGAGCTTG ACCCTAACCT GTACCGCATA GGACAAAGTA AAATCTTCTT CCGAGCTGGA 
GTACTGGCAC ACTTGGAAGA GGAAAGAGAC CTAAAGATCA CTGACATTAT TGTATTTTTC 
CAAGCTGCTG CTCGGGGTTA CCTGGCCAGG AGGGCATTTT ATAAGAAGCA GCAGCAGATG 
AGTGCTCTAA AAGTGGTGCA GCGGAATTGC GCCGCCTACC TCAAGCTCAG ACACTGGCAG 
TGGTGGAGGC TCTTCACCAA GGTAAAGCCA TTACTGCAAG TAACCCGTCA GGATGAGGTC 
ATGCAAGCAA AGGTTGTAGA ACTGCAAAAG GTGAAAGACA CCCAAGTAAA GACTGAATCA 
GAGCTGAAGG AAATGGCTAA CAAGTACCAG CAGTTGTTTG AAGAGAAGAG CATCTTGGCT 
GAGCAGTTAC AAGCAGAAAC AGAGCTTTTT GCCGAAGCAG AAGAAATGCG CGCCAGACTT 
GCATCCAAAA AGCAGGAATT GGAAGAGATT TTGCATGACT TGGAGGCTCG TGTGGAAGAG 
GAGGAAGAAC GTACCTTGCA GCTACAAAAC GAGAAGAAAA AGATGCATCA ACATATACAG 
GATTTGGAAG AACAGCTGGA GGAAGAAGAA GGAGCTCGAC AGAAACTACA GCTAGAAAAA 
GTAACCACAG AGTCTAGGCT GAAGAAAATG GAAGAAGATA TACTCTTACT GGAAGACCAG 
AATGCCAAAC TGGCCAAGGA GAGGAAACTG CTGGATGACC GCATTGGTGA ATTCACTTCA 
ACTATGGCTG AAGAAGAGGA GAAAGTGAAG AGCTTGAATA AATTGCGTAA CAAATACGAA 
GCCGTCATTG CTGACCTGGA AGATCGTCTT AAAAAGGAAG AAAAGGGAAG GCAAGAGATG 
GAGAAGATGA AGAGGAAGCT GGATGGTGAA ACCACTGATT TACAGGATCA GCTGCTAGAG 
CTGCAGCAGC AGATAGAGGA ACTAAAGCAA CAGCTGGCTC GTAAGGAAGA AGAACTCCAA 
GCTGCTCTTG CAAGAGTTGA TGATGAAGTA GGACAAAAGA ACAATTTGCT CAAACAACTT 
CGAGACTTGC AGTCACAGCT CGCTGAGTTA CATGAAGATC TAGAGTCAGA GAAGGCTGCA 
CGAGCAAAGG CAGAAAAGCA GCGTCGGGAC CTTGGTGAGG AGTTGGAGGC CCTGAAAACA 
GAGCTGGAAG ACACTCTCGA CTCCACTGCT GCACAGCAAG AGCTCAGAGC CAAAAGGGAA 
CAAGAGGTGA CAGACCTGAA GAAAACAATA GAGGAAGACG TAAAAGTTCG AGATGCCCAA 
GTAACAGAAA TGCGCCAGCG ACACAACCAA GTGGTGGAAG AGATCTCTGA ACAGCTGGAG 
CAGGCACGAA GGTTCAAGGG GAATCTGGAG AAGGTAAAGC AGACCCTGGA GAGTGAGAAC 
ACTGACCTCA TTAAGGAAGT CAAGAATCTT CAAGCAGCTA AACAGGATTC AGAACAACGC 
AGGAAGAAGC TGGAGCAGCA GGTGTCGGAA TTTCAGATCC GCACCAACGA AAGTGAAAAG 
GTCAAGTTCG AACTGGCGGA GAAACTGCAG AAACTGCAGG CTGAATTAGA TGGTGTATCG 
GGCGCCCTTG GATCAACAGA AGGCAAGTCA ATCAAACTAA CCAAAGACTT GTCAACAGTG 
CAGTCCCAAC TGCAGGATAC CCAGGAGTTG CTTCAGGAGG AAACAAGACA AAAGTTGAAT 
TTTAGCTCTC GAGTTAGGCA GCTGGAGGAG GAAAAGAATA ACCTTATGGA GAACCTAGAG 
GAAGAGGAAT CTGCTAAGGC CCAGCTAAGC CGCCAGTTAC AGGCTTTGCA ACAACAGCTT 
CTCGAGTCTA AGAAGAGGAT GGAGGACCAG GGTGGTATGG TGGAGGCTAT GGAGGAAGCC 
AAGAAGAAAT CATATAAAGA ATTAGAGTTC CTCCAGCAAC GCTTTGACGA GAAACATCAA 
ATAAACGATA AGCTAGAGAA GACAAGAAAC CGCTTACAGC AGGAGCTGGA TGACTTGATG 
GTGGATTTGG ACCATCAACG GCAAATAGTC TCCAATCTGG AGAAAAAGCA GAAGAAGTTT 
GACCAGATGC TAGCTGAAGA GAAAAACATC TCTGCACGTT ATGGAGAAGA GCGAGATCGT 
GCTGAGGCCG AGGCTCGTGA GAAGGAAACA AAGGCCCTGT CTCTAAGTCG GGCCCTGGAA 
GAGGCCATTG ACCTAAAAGA TGAGCTGGAC AGGCAGAACA AGCAATTAAG AGCAGAGATG 
GATGATCTAG TTAGCTCCAA AGATGATGTT GGCAAGAATG TTCATGAGCT GGAAAGGTCT 
AAACGGGCCT TGGAACAGCA AGTTCAGGAA ATGAAGACCC AGATTGAGGA ACTGGAGGAT 
GAGCTACAGG CCATTGAAGA TGGCAAACTT CGCTTGGAGG TCAATATGCA AGCAATGAAA 
GCTCAGTTTG AAAGAGACCT CCAAAACCGA GATGATTCCA ATGATGAAAA GAAGAAGCTG 
CTCTTCAAAC AGGTACGAGA GATGGAAGTG GAGTTGGAAG AGGAAAGGAA ACAGAAATCC 
CAGATTCTTG TTGCCAAGAA AAAGCTTGAA ATGGATCTAC AGGATATGGA AAGCCAGATG 
GACTCTGCTA ACAAAGGTCG GGATGAAGCT GTTAAACAGC TGAAAAAACT ACAGCTCCAG 
TTCAAAGAAG TGTGGCGTGA AGTGGAGGAG ACCAGGGCAG CACGAGACGA GATCTTTGTT 
CAGTCTAGGG ATAATGAGAA GAAACTGAAG AGCCTGGAGG CTGAACTGCT TCAACTACAA 
GAGGACCTGG CAGCCGCCGA ACGTGCAAAG AGACAAGCTC AGCAGGAGAG AGACGACCTG 
GCAGATGAGC TGTCCAATGG AGTCAGTGGC AAATCGGCCC TGCTGGATGA GAAGAGAGCC 
CTGGAGATGC GTATCTCTCA GCTGGAAGAG GAGCTGGATG AAGAGCAGAG TAATACAGAG 
CTTATTAATG ACAGATACAG GAAATTAACT TTGCAGGTGG AGACAATAAC CACTGAGCTC 
TCAGCCGAGC GAAGCTTTTC TCAGAAGGCA GAGAATGCCC GTCAACAAAT GGAGAGGCAG 
AATAAAGAAC TTAAGGTGAA ACTGAATGAG ATGGATTCCA CCATGAGGTC GAAGTACAAG 
ATCACCATCG CCTCTCTTGA GGCCAAGATT AGCCAGCTGG AAGAGCAGAT GGAGCAGGAG 
TCTAAGGAAC GTATCATAGC CAATAAGCTG GTCCGACGTG CTGAAAAGCG GCTTAAGGAG 
GTGCTGCTGC AGGTGGAAGA GGAAAGACGG AATGCAGATC AGTTCAAGGA GCAGCTGGAG 
AAGGCAAATA TACGCATGAA GCAGCTGAAG CGCCAATTGG AGGAGGCCGA GGAAGAGGCT 
TCACGCGCCA ACTCCAACCG CCGCCGGCTG CAGAGAGAGC TGGAAGATGT GACTGAATCA 
GCAGAGTCCA TGAACCGAGA AGTGACCACC CTGAGAAGCA GGCTTAGCAA ATTAGAGCGT 
CAGCAACGCA AGCGGGCACC TATACAGTTC ACCACAAGAA CTATCCGCCA GGTGTATCAA 
CTGGAAGCAG TTTCCGACGA GGAACCCGAA TCCCACAGCG GAGAACCATC TGCCAACCAT 
CAGCAGCAGC AGCCTCCCCA GGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

	MYH10	XP_001118181.2
Rattus norvegicus (Norway rat)	Myh10	NP_113708.1
Gallus gallus (chicken)	MYH10	NP_990805.1
Pan troglodytes (chimpanzee)	MYH10	XP_001166541.2
Homo sapiens (human)	MYH10	NP_001242941.1
Canis lupus familiaris (dog)	MYH10	XP_005620092.1
Caenorhabditis elegans (roundworm)	nmy-1	NP_508504.2
Xenopus tropicalis (tropical clawed frog)	LOC100493774	XP_004916274.1
Mus musculus (house mouse)	Myh10	NP_780469.1
Drosophila melanogaster (fruit fly)	zip	NP_523860.2
Bos taurus (cattle)	MYH10	NP_777259.1
Danio rerio (zebrafish)	myh10	XP_005165776.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007523	XP_308355.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC100493774 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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