robo3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	robo3
Entrez Gene ID	100493553
Full Name	roundabout guidance receptor 3
Synonyms	hgpps,hgps,rbig1,rig1
General protein information	Preferred Names roundabout guidance receptor 3 Names roundabout homolog 3 roundabout, axon guidance receptor, homolog 3 roundabout-3
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002937521.4	XP_002937567.3	roundabout homolog 3 isoform X1
XM_012966981.2	XP_012822435.2	roundabout homolog 3 isoform X2
XM_002937521.3	XP_002937567.2	roundabout homolog 3 isoform X1
XM_012966982.3	XP_012822436.2	roundabout homolog 3 isoform X3
XM_012966982.2	XP_012822436.1	roundabout homolog 3 isoform X4
XM_012966981.1	XP_012822435.1	roundabout homolog 3 isoform X2
XM_018095924.1	XP_017951413.1	roundabout homolog 3 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ROBO3	XP_003313454.2
Canis lupus familiaris (dog)	ROBO3	XP_005619673.1
Danio rerio (zebrafish)	robo3	NP_571557.1
	ROBO3	XP_001106913.2
Mus musculus (house mouse)	Robo3	NP_001158239.1
Bos taurus (cattle)	ROBO3	XP_002699249.1
Rattus norvegicus (Norway rat)	Robo3	NP_001101605.1
Gallus gallus (chicken)	ROBO3	XP_003642645.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010970	XP_309724.3
Homo sapiens (human)	ROBO3	NP_071765.2
Xenopus tropicalis (tropical clawed frog)	robo3	XP_002937567.2

Gene Ontology

The following robo3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robo3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45809	XM_018095924.1 Latest version!	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa61798	XM_002937521.4 Latest version!	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61799	XM_012966981.2 Latest version!	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa61800	XM_012966982.3 Latest version!	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa13009	XM_002937521.3 Latest version!	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa35708	XM_012966981.1 Latest version!	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa35709	XM_012966982.2 Latest version!	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

robo3 ( XM_018095924.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951413.1 Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa45809
Clone ID Related Accession (Same CDS sequence)	XM_018095924.1
Accession Version	XM_018095924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGC AGAAGATGAG CTTTATGGGC AAAACCTTCA CCTGCGTTTT ATTTGTGTCC 
GTATGTTCCG TCCTAGGTTC TAGACCTCGA CTAGAAGATT TCCCGCCTCG AATAGTGGAG 
CATCCTTCTG ATCACATTGT CTCAAAGGGG GAACCAGCTA CTTTAAACTG CAAAGCAGAA 
GGCCGACCAA CACCTATTAT TGAGTGGTAC AAGGATGGGG AAAGGGTGGA GACAGACAAA 
GACGATCCTC GGTCACATCG CATGCTGTTA CCCAGTGGCT CACTTTTCTT TCTACGAATT 
GTTCATGGCC GTCGGAGTAA ACCCGATGAA GGGGTATACA TTTGTGTTGC AAGAAATTAC 
CTTGGAGAGT CTGTCAGTCG AAATGCATCT CTGGAAGTAG CAATATTAAG AGATGATTTT 
CGTCAGGCAC CCAATGATGT GGTAGTAGCA GCAGGAGAAC CAGCAGTCAT GGAATGCATT 
CCACCCAGAG GTCATCCTGA ACCCACTATT TCCTGGAAAA AGGATAACAT GCGCATAAGT 
GATAAAGATG AACGCATCAC AATTCGAGGT GGGAAACTAA TGATTTCCAA TACGAGAAAG 
AGCGATGCAG GCATGTACAT CTGTGTGGCT ACAAACATGG TGGGAGAACG AGATAGCGAA 
CCTGCTCAGC TTGTGGTCTT TGAACGCCCC ACATTTATTA GAAGGCCCAT AAACCAAGTT 
GTTCTAGCAG ATGATATGGT GGATTTTAAC TGTGAAGTTC AGGGTGATCC TGTACCTACT 
GCTCGCTGGC GTAAGGAAGA AGGAGAACTA CCACGGGGCC GGTATGAAAT CCTTAATGAT 
AATACATTGC GGATCTCTCG GGTGAGTGCT GAGGATGAAG GAACTTACAC TTGTATCACT 
GAAAATAGTG TTGGAAAATC AGAGGCTTCA GGATCTCTTG GCGTTCATGT TGGCCCATTT 
TTTCCTCCAC AACTGGTCAC CCGACCACGA GACCAAATAG TAGTTCAAGG ACGTACTGTT 
ACATTCCAGT GTGAAACCAA AGGGAATCCA CCACCAGCTG TATTTTGGCA GAAAGAAGGA 
AGTCAGATCC TTCTTTTCCC AAGCCAGCCT CCCCAAACAG TGGGACGATT TTCTGTCTCT 
CCGAGTGGAG AACTGACCAT AATCGATGTT CAGACAGGAG ACTCAGGGTA CTACATGTGT 
CAAGCCATTA GTGTGGCTGG AAGTATCTTA GCTAAGGCTC TACTTGAAGT GGAAGATGCA 
ACATCAGATC GAATTCCTCC TATAATTCGT AGAGGACCAG CCAATCAAAC CCTTGCTGTT 
GACTCTACAG TGCAGATGCA TTGCCAGGTA TCTGGTAATC CTCTTCCAAG TATACATTGG 
CTCAAAGATG GCCAAAGGAT TGTGGGAAGT GACCCTCGAA TAGGAGTGTT GGACAGTGGA 
AATTTACAGA TCACCCATTT ACAGGTGACT GATTCAGGTG TATATACATG TGTGGCTACA 
AGTTCAACTG GGGAGACTAC ATGGAGTGGA TTTGTTGAAG TTCAAGAAAA AGGATCTCCA 
ATACATGTGC AGCTTTCTGA GCCCAGCCTT GTTCCTGGAT CTCCTTCCAA ACCTCTTGTC 
ACTGACGTTA CCAAAAATAG TGTTACTTTA ACATGGCAAC CCAATCTGCA AGCAGGAAAC 
ACACAAGTTA ATTCCTATAT CATTGAAGCA TTCAGCCAAT CAGCAGGAAG CACCTGGCAA 
ACAGTTGCAG ACAACGTGAA AGTAGAAAAG CACACGGTGG TTGGGTTGAA CCCAAATACA 
ATTTATCTTT TCATCATACG AGCAGTAAAT TCATATGGAC TTAGTGACCC CAGCCCAATT 
TCAGAACCAG TGCGAACACA AGATGTCAGT CCAACGAGAC AAGGTGTTGA TCATAGGCAA 
GTGCAGCGCC AACTAGGGGA AGTGGTGGTT CAGCTTCAAC AACCGATTAT TCTTACATCC 
TCTACTGTGC AAGTTACATG GACAGTGGAT CGGCAAGCTC AGTTCATCCA AGGTTACCGG 
GTGATGTATC GTCCAAAATC TAGTGTTTGG CTTGTACAGG ATGTGAAGTC TCCCTCTGAA 
CGGAATACTA TCCTTGTGGA CCTCAAAAAA GGCACAGAAT ATGAAATCAA GATCCGTCCT 
TATTTTGATG AATTTCAAGG CATGGACAGT GATGTGCTTT TGATCCGTAC ACCTGAAGAA 
GTGCCAAGTG CCCCGCCTCA AGCAGTATCA GTTGTTACAT TAGGAACGAA TAACAGCACA 
AATATTAGTA TATCGTGGGA ACCTCCTATA GTGGAACATC AAAATGGAAT TATTCAAGAT 
TATCGGATTT GGGTCTTGGG AAATGAAACA CGCTATCATA TCAACAGAAC AGTAGATGGT 
ACTGTCCACT CCACTGTAAT AAGAGGCCTC AGTCCTGGAG TTCTTTACCA TGTGGAAGTG 
GCTGCTGCAA CAAGTGCTGG TTTTGGTGTA AAAAGCCAAC CAATCACCAT CCAGATAAAG 
CCTTCTTCAG ATCAAGTTCC AGTTGCCAGT GGAGAAAATG TCAGCTTGAC TGAACAAATC 
ACAGATGTTG TTAAACAGCC TGCATTCATA GCTGGCATTG GAGGGGCATG CTGGGTAATT 
CTAATGTGCT TTAGTGTCTG GATCTACTGC CGAAGGAAGA AGAGGAAAGA ACTGAGCCAT 
TATACTGCTT CCTTTGCATA CACACCAGCA GTATCTTTTC CAGAAAGCTC AGGGCATATT 
ACTGCAAGAC CAGGAATGCT AGCTACAAGT GCATCCAACT ACCCTTGGCT AGCAGATTCA 
TGGCCAGCAA CCAACTTACT ACGAAACGGA AAGCCAAAAG AACCTGCAGG GAATTGTTGT 
CCAGGAAATC ACAATCCTTC AGAGAGATAT TATAATGATG CTGGCATTTC AAACTATATT 
GCTCAGACTG AGAAGTTCGG AACAGGTGTT TCAGATGGTC CCATATACAG TACAATTGAT 
CCAACCACTG ATGACATGCG AACTTTTAAT GGAGCTTTCT CTCAGCATAC TACACCTTAT 
GCAACAACAC CTGTTGTACC GTATGGAGGA CAAAACATGC ATTCTCCTGA ATTAGAAGAG 
AACCAGTGGA ATGTACAGGC TGGAACATCT GGTGTACTTT ATGCACAACC AGATCGAACT 
AAAGCAGAGA ATGCTATATG CATGTTGGCC TTTTCAGGTC CCAAATCTGG AAAGCAGAAG 
GCCATTGGAA AACCAGTAAA AACACCATCA CTAAATTGGA CAGAAATTCT TCCACCACCC 
CCGCCTGCCA ATGAGCTGAG TCAATACCAA GAAGAAGATA CAGATATAGA GATGAGCTCC 
GACACAGAAG AATGGTGTCC ACCATTGCCT GAAAGAACTT ACCTCATGGA AAACATTCAA 
GAAGGCTGCC CACTTCCTCC ACCTCGGGGT GAGGCTTCTT CTCCTGCTCA TTCTTACGGT 
CAGCAGTCAA CAGCCACTCT AACACCATCT CCCCAGGATG AGATGCATGC TGCAGAAGAT 
ATTCCAAGGC TACATCAGTT TGAGATACCC CCTCATCCTA GACGACTGCC ATCTGGATCG 
GTACCAATTC CAAGAGCTCC AAGTCCTCCT CTGACACAAT CAAATCCTAG TCTTCATACA 
GCAACGGAAG GTGCAGAAGC ACAACGGCAG GGACACAGGC GGAACTTAAG CCAAAATCCT 
AATCTAAGTG CTGAAAATGT CAACTTGCCA AACAGCGGAA AGTCACAACT AGGAGAAAAT 
ATAAGCACAC CTCCAGGACA GAAATCGCGA GCAAAGAAGA AGTCAAAATC AGGTCACTAT 
CGCAGGGAAG CAAAACAAGG AGATCTTCCT CCACCACCTG AGCCCCCTCC AGCAGATGAG 
GAACCATGTT CTCCACACAC TAATGGACTA CGTATGGAAT GTTCAGCCTC GTCCTTGGAA 
AGGGATACAA GTACTTCTTC AGTTGAGCGG AGAAATGGGG TATTACAACA GCGAGGAGCA 
GCAGGACCTC AATTCCATCA GAGAGATGAT GAAGAACTAA TTCCTTACAG TAAACCTAAC 
TTCCTATCAC GGAGTCAGAT GTCAAGTAAT TGTTCCACAA CAGGAAGCAG CTCCTCCAGA 
GGGTCAGCTG GATCTAGAGG TCAGGCATCT GGAAGGAGCA AAACAGGAGA CCGGAAAGAG 
GAAAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951413.1
CDS	517..4728
Translation	MERQKMSFMGKTFTCVLFVSVCSVLGSRPRLEDFPPRIVEHPSDHIVSKGEPATLNCKAEGRPTPIIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYICVARNYLGESVSRNASLEVAILRDDFRQAPNDVVVAAGEPAVMECIPPRGHPEPTISWKKDNMRISDKDERITIRGGKLMISNTRKSDAGMYICVATNMVGERDSEPAQLVVFERPTFIRRPINQVVLADDMVDFNCEVQGDPVPTARWRKEEGELPRGRYEILNDNTLRISRVSAEDEGTYTCITENSVGKSEASGSLGVHVGPFFPPQLVTRPRDQIVVQGRTVTFQCETKGNPPPAVFWQKEGSQILLFPSQPPQTVGRFSVSPSGELTIIDVQTGDSGYYMCQAISVAGSILAKALLEVEDATSDRIPPIIRRGPANQTLAVDSTVQMHCQVSGNPLPSIHWLKDGQRIVGSDPRIGVLDSGNLQITHLQVTDSGVYTCVATSSTGETTWSGFVEVQEKGSPIHVQLSEPSLVPGSPSKPLVTDVTKNSVTLTWQPNLQAGNTQVNSYIIEAFSQSAGSTWQTVADNVKVEKHTVVGLNPNTIYLFIIRAVNSYGLSDPSPISEPVRTQDVSPTRQGVDHRQVQRQLGEVVVQLQQPIILTSSTVQVTWTVDRQAQFIQGYRVMYRPKSSVWLVQDVKSPSERNTILVDLKKGTEYEIKIRPYFDEFQGMDSDVLLIRTPEEVPSAPPQAVSVVTLGTNNSTNISISWEPPIVEHQNGIIQDYRIWVLGNETRYHINRTVDGTVHSTVIRGLSPGVLYHVEVAAATSAGFGVKSQPITIQIKPSSDQVPVASGENVSLTEQITDVVKQPAFIAGIGGACWVILMCFSVWIYCRRKKRKELSHYTASFAYTPAVSFPESSGHITARPGMLATSASNYPWLADSWPATNLLRNGKPKEPAGNCCPGNHNPSERYYNDAGISNYIAQTEKFGTGVSDGPIYSTIDPTTDDMRTFNGAFSQHTTPYATTPVVPYGGQNMHSPELEENQWNVQAGTSGVLYAQPDRTKAENAICMLAFSGPKSGKQKAIGKPVKTPSLNWTEILPPPPPANELSQYQEEDTDIEMSSDTEEWCPPLPERTYLMENIQEGCPLPPPRGEASSPAHSYGQQSTATLTPSPQDEMHAAEDIPRLHQFEIPPHPRRLPSGSVPIPRAPSPPLTQSNPSLHTATEGAEAQRQGHRRNLSQNPNLSAENVNLPNSGKSQLGENISTPPGQKSRAKKKSKSGHYRREAKQGDLPPPPEPPPADEEPCSPHTNGLRMECSASSLERDTSTSSVERRNGVLQQRGAAGPQFHQRDDEELIPYSKPNFLSRSQMSSNCSTTGSSSSRGSAGSRGQASGRSKTGDRKEEKR

Target ORF information:

RefSeq Version	XM_018095924.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095924.1

ORF Insert Sequence:

ATGGAAAGGC AGAAGATGAG CTTTATGGGC AAAACCTTCA CCTGCGTTTT ATTTGTGTCC 
GTATGTTCCG TCCTAGGTTC TAGACCTCGA CTAGAAGATT TCCCGCCTCG AATAGTGGAG 
CATCCTTCTG ATCACATTGT CTCAAAGGGG GAACCAGCTA CTTTAAACTG CAAAGCAGAA 
GGCCGACCAA CACCTATTAT TGAGTGGTAC AAGGATGGGG AAAGGGTGGA GACAGACAAA 
GACGATCCTC GGTCACATCG CATGCTGTTA CCCAGTGGCT CACTTTTCTT TCTACGAATT 
GTTCATGGCC GTCGGAGTAA ACCCGATGAA GGGGTATACA TTTGTGTTGC AAGAAATTAC 
CTTGGAGAGT CTGTCAGTCG AAATGCATCT CTGGAAGTAG CAATATTAAG AGATGATTTT 
CGTCAGGCAC CCAATGATGT GGTAGTAGCA GCAGGAGAAC CAGCAGTCAT GGAATGCATT 
CCACCCAGAG GTCATCCTGA ACCCACTATT TCCTGGAAAA AGGATAACAT GCGCATAAGT 
GATAAAGATG AACGCATCAC AATTCGAGGT GGGAAACTAA TGATTTCCAA TACGAGAAAG 
AGCGATGCAG GCATGTACAT CTGTGTGGCT ACAAACATGG TGGGAGAACG AGATAGCGAA 
CCTGCTCAGC TTGTGGTCTT TGAACGCCCC ACATTTATTA GAAGGCCCAT AAACCAAGTT 
GTTCTAGCAG ATGATATGGT GGATTTTAAC TGTGAAGTTC AGGGTGATCC TGTACCTACT 
GCTCGCTGGC GTAAGGAAGA AGGAGAACTA CCACGGGGCC GGTATGAAAT CCTTAATGAT 
AATACATTGC GGATCTCTCG GGTGAGTGCT GAGGATGAAG GAACTTACAC TTGTATCACT 
GAAAATAGTG TTGGAAAATC AGAGGCTTCA GGATCTCTTG GCGTTCATGT TGGCCCATTT 
TTTCCTCCAC AACTGGTCAC CCGACCACGA GACCAAATAG TAGTTCAAGG ACGTACTGTT 
ACATTCCAGT GTGAAACCAA AGGGAATCCA CCACCAGCTG TATTTTGGCA GAAAGAAGGA 
AGTCAGATCC TTCTTTTCCC AAGCCAGCCT CCCCAAACAG TGGGACGATT TTCTGTCTCT 
CCGAGTGGAG AACTGACCAT AATCGATGTT CAGACAGGAG ACTCAGGGTA CTACATGTGT 
CAAGCCATTA GTGTGGCTGG AAGTATCTTA GCTAAGGCTC TACTTGAAGT GGAAGATGCA 
ACATCAGATC GAATTCCTCC TATAATTCGT AGAGGACCAG CCAATCAAAC CCTTGCTGTT 
GACTCTACAG TGCAGATGCA TTGCCAGGTA TCTGGTAATC CTCTTCCAAG TATACATTGG 
CTCAAAGATG GCCAAAGGAT TGTGGGAAGT GACCCTCGAA TAGGAGTGTT GGACAGTGGA 
AATTTACAGA TCACCCATTT ACAGGTGACT GATTCAGGTG TATATACATG TGTGGCTACA 
AGTTCAACTG GGGAGACTAC ATGGAGTGGA TTTGTTGAAG TTCAAGAAAA AGGATCTCCA 
ATACATGTGC AGCTTTCTGA GCCCAGCCTT GTTCCTGGAT CTCCTTCCAA ACCTCTTGTC 
ACTGACGTTA CCAAAAATAG TGTTACTTTA ACATGGCAAC CCAATCTGCA AGCAGGAAAC 
ACACAAGTTA ATTCCTATAT CATTGAAGCA TTCAGCCAAT CAGCAGGAAG CACCTGGCAA 
ACAGTTGCAG ACAACGTGAA AGTAGAAAAG CACACGGTGG TTGGGTTGAA CCCAAATACA 
ATTTATCTTT TCATCATACG AGCAGTAAAT TCATATGGAC TTAGTGACCC CAGCCCAATT 
TCAGAACCAG TGCGAACACA AGATGTCAGT CCAACGAGAC AAGGTGTTGA TCATAGGCAA 
GTGCAGCGCC AACTAGGGGA AGTGGTGGTT CAGCTTCAAC AACCGATTAT TCTTACATCC 
TCTACTGTGC AAGTTACATG GACAGTGGAT CGGCAAGCTC AGTTCATCCA AGGTTACCGG 
GTGATGTATC GTCCAAAATC TAGTGTTTGG CTTGTACAGG ATGTGAAGTC TCCCTCTGAA 
CGGAATACTA TCCTTGTGGA CCTCAAAAAA GGCACAGAAT ATGAAATCAA GATCCGTCCT 
TATTTTGATG AATTTCAAGG CATGGACAGT GATGTGCTTT TGATCCGTAC ACCTGAAGAA 
GTGCCAAGTG CCCCGCCTCA AGCAGTATCA GTTGTTACAT TAGGAACGAA TAACAGCACA 
AATATTAGTA TATCGTGGGA ACCTCCTATA GTGGAACATC AAAATGGAAT TATTCAAGAT 
TATCGGATTT GGGTCTTGGG AAATGAAACA CGCTATCATA TCAACAGAAC AGTAGATGGT 
ACTGTCCACT CCACTGTAAT AAGAGGCCTC AGTCCTGGAG TTCTTTACCA TGTGGAAGTG 
GCTGCTGCAA CAAGTGCTGG TTTTGGTGTA AAAAGCCAAC CAATCACCAT CCAGATAAAG 
CCTTCTTCAG ATCAAGTTCC AGTTGCCAGT GGAGAAAATG TCAGCTTGAC TGAACAAATC 
ACAGATGTTG TTAAACAGCC TGCATTCATA GCTGGCATTG GAGGGGCATG CTGGGTAATT 
CTAATGTGCT TTAGTGTCTG GATCTACTGC CGAAGGAAGA AGAGGAAAGA ACTGAGCCAT 
TATACTGCTT CCTTTGCATA CACACCAGCA GTATCTTTTC CAGAAAGCTC AGGGCATATT 
ACTGCAAGAC CAGGAATGCT AGCTACAAGT GCATCCAACT ACCCTTGGCT AGCAGATTCA 
TGGCCAGCAA CCAACTTACT ACGAAACGGA AAGCCAAAAG AACCTGCAGG GAATTGTTGT 
CCAGGAAATC ACAATCCTTC AGAGAGATAT TATAATGATG CTGGCATTTC AAACTATATT 
GCTCAGACTG AGAAGTTCGG AACAGGTGTT TCAGATGGTC CCATATACAG TACAATTGAT 
CCAACCACTG ATGACATGCG AACTTTTAAT GGAGCTTTCT CTCAGCATAC TACACCTTAT 
GCAACAACAC CTGTTGTACC GTATGGAGGA CAAAACATGC ATTCTCCTGA ATTAGAAGAG 
AACCAGTGGA ATGTACAGGC TGGAACATCT GGTGTACTTT ATGCACAACC AGATCGAACT 
AAAGCAGAGA ATGCTATATG CATGTTGGCC TTTTCAGGTC CCAAATCTGG AAAGCAGAAG 
GCCATTGGAA AACCAGTAAA AACACCATCA CTAAATTGGA CAGAAATTCT TCCACCACCC 
CCGCCTGCCA ATGAGCTGAG TCAATACCAA GAAGAAGATA CAGATATAGA GATGAGCTCC 
GACACAGAAG AATGGTGTCC ACCATTGCCT GAAAGAACTT ACCTCATGGA AAACATTCAA 
GAAGGCTGCC CACTTCCTCC ACCTCGGGGT GAGGCTTCTT CTCCTGCTCA TTCTTACGGT 
CAGCAGTCAA CAGCCACTCT AACACCATCT CCCCAGGATG AGATGCATGC TGCAGAAGAT 
ATTCCAAGGC TACATCAGTT TGAGATACCC CCTCATCCTA GACGACTGCC ATCTGGATCG 
GTACCAATTC CAAGAGCTCC AAGTCCTCCT CTGACACAAT CAAATCCTAG TCTTCATACA 
GCAACGGAAG GTGCAGAAGC ACAACGGCAG GGACACAGGC GGAACTTAAG CCAAAATCCT 
AATCTAAGTG CTGAAAATGT CAACTTGCCA AACAGCGGAA AGTCACAACT AGGAGAAAAT 
ATAAGCACAC CTCCAGGACA GAAATCGCGA GCAAAGAAGA AGTCAAAATC AGGTCACTAT 
CGCAGGGAAG CAAAACAAGG AGATCTTCCT CCACCACCTG AGCCCCCTCC AGCAGATGAG 
GAACCATGTT CTCCACACAC TAATGGACTA CGTATGGAAT GTTCAGCCTC GTCCTTGGAA 
AGGGATACAA GTACTTCTTC AGTTGAGCGG AGAAATGGGG TATTACAACA GCGAGGAGCA 
GCAGGACCTC AATTCCATCA GAGAGATGAT GAAGAACTAA TTCCTTACAG TAAACCTAAC 
TTCCTATCAC GGAGTCAGAT GTCAAGTAAT TGTTCCACAA CAGGAAGCAG CTCCTCCAGA 
GGGTCAGCTG GATCTAGAGG TCAGGCATCT GGAAGGAGCA AAACAGGAGA CCGGAAAGAG 
GAAAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_002937521.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937567.3 Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa61798
Clone ID Related Accession (Same CDS sequence)	XM_002937521.4
Accession Version	XM_002937521.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937521.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ATCTGCTAAA GACTCTGCTG CAGATGAACC TCTTTGCGGA CTCCATCCCA 
GGCGAGAACT CCAATTCCAC TGATTTATTT TACCAGTTTA ACTCATCCTT TCCTGCTTTC 
AATGACAGCT TAATTGACTT CATCAACCTG ACCAACTTCA ATGGTTCTAG ACCCCGACTA 
GAAGATTTCC CGCCTCGAAT AGTGGAGCAT CCTTCTGATC ACATCGTCTC AAAGGGGGAA 
CCAGCTACTT TAAACTGCAA AGCAGAAGGC CGACCAACAC CTATTATTGA GTGGTACAAG 
GATGGGGAAA GGGTGGAGAC AGACAAAGAC GATCCTCGGT CACATCGCAT GCTGTTACCC 
AGTGGCTCAC TTTTCTTTCT ACGAATTGTT CATGGCCGTC GGAGTAAACC CGATGAAGGG 
GTATACATTT GTGTTGCAAG AAATTACCTT GGAGAGTCTG TCAGTCGAAA TGCATCTCTG 
GAAGTAGCAA TATTAAGAGA TGATTTTCGT CAGGCACCCA ATGATGTGGT AGTAGCAGCA 
GGAGAACCAG CAGTCATGGA ATGCATTCCA CCCAGAGGTC ATCCTGAACC CACTATTTCC 
TGGAAAAAGG ATAACATGCG CATAAGTGAT AAAGATGAAC GCATCACAAT TCGAGGTGGG 
AAACTAATGA TTTCCAATAC GAGAAAGAGC GATGCAGGCA TGTACATCTG TGTGGCTACA 
AACATGGTGG GAGAACGAGA TAGCGAACCT GCTCAGCTTG TGGTCTTTGA ACGCCCCACA 
TTTATTAGAA GGCCCATAAA CCAAGTTGTT CTAGCAGATG ATATGGTGGA TTTTAACTGT 
GAAGTTCAGG GTGATCCTGT ACCTACTGCT CGCTGGCGTA AGGAAGAAGG AGAACTACCA 
CGGGGCCGGT ATGAAATCCT TAATGATAAT ACATTGCGGA TCTCTCGGGT GAGTGCTGAG 
GATGAAGGAA CTTACACTTG TATCACTGAA AATAGTGTTG GAAAATCAGA GGCTTCAGGA 
TCTCTTGGCG TTCATGTTGG CCCATTTTTT CCTCCACAAC TGGTCACCCG ACCACGAGAC 
CAAATAGTAG TTCAAGGACG TACTGTTACA TTCCAGTGTG AAACCAAAGG GAATCCACCA 
CCAGCTGTAT TTTGGCAGAA AGAAGGAAGT CAGATCCTTC TTTTCCCAAG CCAGCCTCCC 
CAAACAGTGG GACGATTTTC TGTCTCTCCG AGTGGAGAAC TGACCATAAT CGATGTTCAG 
ACAGGAGACT CAGGGTACTA CATGTGTCAA GCCATTAGTG TGGCTGGAAG TATCTTAGCT 
AAGGCTCTAC TTGAAGTGGA AGATGCAACA TCAGATCGAA TTCCTCCTAT AATTCGTAGA 
GGACCAGCCA ATCAAACCCT TGCTGTTGAC TCTACAGTGC AGATGCATTG CCAGGTATCT 
GGTAATCCTC TTCCAAGTAT ACATTGGCTC AAAGATGGCC AAAGGATTGT GGGAAGTGAC 
CCTCGAATAG GAGTGTTGGA CAGTGGAAAT TTACAGATCA CCCATTTACA GGTGACTGAT 
TCAGGTGTAT ATACATGTGT GGCTACAAGT TCAACTGGGG AGACTACATG GAGTGGATTT 
GTTGAAGTTC AAGAAAAAGG ATCTCCAATA CATGTGCAGC TTTCTGAGCC CAGCCTTGTT 
CCTGGATCTC CTTCCAAACC TCTTGTCACT GACGTTACCA AAAATAGTGT TACTTTAACA 
TGGCAACCCA ATCTGCAAGC AGGAAACACA CAAGTTAATT CCTATATCAT TGAAGCATTC 
AGCCAATCAG CAGGAAGCAC CTGGCAAACA GTTGCAGACA ACGTGAAAGT AGAAAAGCAC 
ACGGTGGTTG GGTTGAACCC AAATACAATT TATCTTTTCA TCATACGAGC AGTAAATTCA 
TATGGACTTA GTGACCCCAG CCCAATTTCA GAACCAGTGC GAACACAAGA TGTCAGTCCA 
ACGAGACAAG GTGTTGATCA TAGGCAAGTG CAGCGCCAAC TAGGGGAAGT GGTGGTTCAG 
CTTCAACAAC CGATTATTCT TACATCCTCT ACTGTGCAAG TTACATGGAC AGTGGATCGG 
CAAGCTCAGT TCATCCAAGG TTACCGGGTG ATGTATCGTC CAAAATCTAG TGTTTGGCTT 
GTACAGGATG TGAAGTCTCC CTCTGAACGG AATACTATCC TTGTGGACCT CAAAAAAGGC 
ACAGAATATG AAATCAAGAT CCGTCCTTAT TTTGATGAAT TTCAAGGCAT GGACAGTGAT 
GTGCTTTTGA TCCGTACACC TGAAGAAGTG CCAAGTGCCC CGCCTCAAGC AGTATCAGTT 
GTTACATTAG GAACGAATAA CAGCACAAAT ATTAGTATAT CGTGGGAACC TCCTATAGTG 
GAACATCAAA ATGGAATTAT TCAAGATTAT CGGATTTGGG TCTTGGGAAA TGAAACACGC 
TATCATATCA ACAGAACAGT AGATGGTACT GTCCACTCCA CTGTAATAAG AGGCCTTAGT 
CCTGGAGTTC TTTACCATGT GGAAGTGGCT GCTGCAACAA GTGCTGGTTT TGGTGTAAAA 
AGCCAACCAA TCACCATCCA GATAAAGCCT TCTTCAGATC AAGTTCCAGT TGCCAGTGGA 
GAAAATGTCA GCTTGACTGA ACAAATCACA GATGTTGTTA AACAGCCTGC ATTCATAGCT 
GGCATTGGAG GGGCATGCTG GGTAATTCTA ATGTGCTTTA GTGTCTGGAT CTACTGCCGA 
AGGAAGAAGA GGAAAGAACT GAGCCATTAT ACTGCTTCCT TTGCATACAC ACCAGCAGTA 
TCTTTTCCAG AAAGCTCAGG GCATATTACT GCAAGACCAG GAATGCTAGC TACAAGTGCA 
TCCAACTACC CTTGGCTAGC AGATTCATGG CCAGCAACCA ACTTACTACG AAACGGAAAG 
CCAAAAGAAC CTGCAGGGAA TTGTTGTCCA GGAAATCACA ATCCTTCAGA GAGATATTAT 
AATGATGCTG GCATTTCAAA CTATATTGCT CAGACTGAGA AGTTCGGAAC AGGTGTTTCA 
GATGGTCCCA TATACAGTAC AATTGATCCA ACCACTGATG ACATGCGAAC TTTTAATGGA 
GCTTTCTCTC AGCATACTAC ACCTTATGCA ACAACACCTG TTGTACCGTA TGGAGGACAA 
AACATGCATT CTCCTGAATT AGAAGAGAAC CAGTGGAATG TACAGGCTGG AACATCTGGT 
GTACTTTATG CACAACCAGA TCGAACTAAA GCAGAGAATG GTCCCAAATC TGGAAAGCAG 
AAGGCCATTG GAAAACCAGT AAAAACACCA TCACTAAATT GGACAGAAAT TCTTCCACCA 
CCCCCGCCTG CCAATGAGCT GAGTCAATAC CAAGAAGAAG ATACAGATGT AGAGATGAGC 
TCCGACACAG AAGAATGGTG TCCACCATTG CCTGAAAGAA CTTACCTCAT GGAAAACATT 
CAAGAAGGCT GCCCACTTCC TCCACCTCGG GGTGAGGCTT CTTCTCCTGC TCATTCTTAC 
GGTCAGCAGT CAACAGCCAC TCTAACACCA TCTCCCCAGG ATGAGATGCA TGCTGCAGAA 
GATATTCCAA GGCTACATCA GTTTGAGATA CCCCCTCATC CTAGACGACT GCCATCTGGA 
TCGGTACCAA TTCCAAGAGC TCCAAGTCCT CCTCTGACAC AATCAAATCC TAGTCTTCAT 
ACAGCAACGG AAGGTGCAGA AGCACAACGG CAGGGACACA GGCGGAACTT AAGCCAAAAT 
CCTAATCTAA GTGCTGAAAA TGTCAACTTG CCAAACAGCG GAAAGTCACA ACTAGGAGAA 
AATATAAGCA CACCTCCAGG ACAGAAATCG CGAGCAAAGA AGAAGTCAAA ATCAGGTCAC 
TATCGCAGGG AAGCAAAACA AGGAGATCTT CCTCCACCAC CTGAGCCCCC TCCAGCAGAT 
GAGGAACCAT GTTCTCCACA CACTAATGGA CTACGTATGG AATGTTCAGC CTCGTCCTTG 
GAAAGGGATA CAAGTACTTC TTCAGTTGAG CGGAGAAATG GGGTATTACA ACAGCGAGGA 
GCAGCAGGAC CTCAATTCCA TCAGAGAGAT GATGAAGAAC TAATTCCTTA CAGTAAACCT 
AACTTCCTAT CACGGAGTCA GATGTCAAGT AATTGTTCCA CAACAGGAAG CAGCTCCTCC 
AGAGGGTCAG CTGGATCTAG AGGTCAGGCA TCTGGAAGGA GCAAAACAGG AGACCGGAAA 
GAGGAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937567.3
CDS	362..4636
Translation	MLRYLLKTLLQMNLFADSIPGENSNSTDLFYQFNSSFPAFNDSLIDFINLTNFNGSRPRLEDFPPRIVEHPSDHIVSKGEPATLNCKAEGRPTPIIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYICVARNYLGESVSRNASLEVAILRDDFRQAPNDVVVAAGEPAVMECIPPRGHPEPTISWKKDNMRISDKDERITIRGGKLMISNTRKSDAGMYICVATNMVGERDSEPAQLVVFERPTFIRRPINQVVLADDMVDFNCEVQGDPVPTARWRKEEGELPRGRYEILNDNTLRISRVSAEDEGTYTCITENSVGKSEASGSLGVHVGPFFPPQLVTRPRDQIVVQGRTVTFQCETKGNPPPAVFWQKEGSQILLFPSQPPQTVGRFSVSPSGELTIIDVQTGDSGYYMCQAISVAGSILAKALLEVEDATSDRIPPIIRRGPANQTLAVDSTVQMHCQVSGNPLPSIHWLKDGQRIVGSDPRIGVLDSGNLQITHLQVTDSGVYTCVATSSTGETTWSGFVEVQEKGSPIHVQLSEPSLVPGSPSKPLVTDVTKNSVTLTWQPNLQAGNTQVNSYIIEAFSQSAGSTWQTVADNVKVEKHTVVGLNPNTIYLFIIRAVNSYGLSDPSPISEPVRTQDVSPTRQGVDHRQVQRQLGEVVVQLQQPIILTSSTVQVTWTVDRQAQFIQGYRVMYRPKSSVWLVQDVKSPSERNTILVDLKKGTEYEIKIRPYFDEFQGMDSDVLLIRTPEEVPSAPPQAVSVVTLGTNNSTNISISWEPPIVEHQNGIIQDYRIWVLGNETRYHINRTVDGTVHSTVIRGLSPGVLYHVEVAAATSAGFGVKSQPITIQIKPSSDQVPVASGENVSLTEQITDVVKQPAFIAGIGGACWVILMCFSVWIYCRRKKRKELSHYTASFAYTPAVSFPESSGHITARPGMLATSASNYPWLADSWPATNLLRNGKPKEPAGNCCPGNHNPSERYYNDAGISNYIAQTEKFGTGVSDGPIYSTIDPTTDDMRTFNGAFSQHTTPYATTPVVPYGGQNMHSPELEENQWNVQAGTSGVLYAQPDRTKAENGPKSGKQKAIGKPVKTPSLNWTEILPPPPPANELSQYQEEDTDVEMSSDTEEWCPPLPERTYLMENIQEGCPLPPPRGEASSPAHSYGQQSTATLTPSPQDEMHAAEDIPRLHQFEIPPHPRRLPSGSVPIPRAPSPPLTQSNPSLHTATEGAEAQRQGHRRNLSQNPNLSAENVNLPNSGKSQLGENISTPPGQKSRAKKKSKSGHYRREAKQGDLPPPPEPPPADEEPCSPHTNGLRMECSASSLERDTSTSSVERRNGVLQQRGAAGPQFHQRDDEELIPYSKPNFLSRSQMSSNCSTTGSSSSRGSAGSRGQASGRSKTGDRKEEKR

Target ORF information:

RefSeq Version	XM_002937521.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937521.4

ORF Insert Sequence:

ATGCTGCGCT ATCTGCTAAA GACTCTGCTG CAGATGAACC TCTTTGCGGA CTCCATCCCA 
GGCGAGAACT CCAATTCCAC TGATTTATTT TACCAGTTTA ACTCATCCTT TCCTGCTTTC 
AATGACAGCT TAATTGACTT CATCAACCTG ACCAACTTCA ATGGTTCTAG ACCCCGACTA 
GAAGATTTCC CGCCTCGAAT AGTGGAGCAT CCTTCTGATC ACATCGTCTC AAAGGGGGAA 
CCAGCTACTT TAAACTGCAA AGCAGAAGGC CGACCAACAC CTATTATTGA GTGGTACAAG 
GATGGGGAAA GGGTGGAGAC AGACAAAGAC GATCCTCGGT CACATCGCAT GCTGTTACCC 
AGTGGCTCAC TTTTCTTTCT ACGAATTGTT CATGGCCGTC GGAGTAAACC CGATGAAGGG 
GTATACATTT GTGTTGCAAG AAATTACCTT GGAGAGTCTG TCAGTCGAAA TGCATCTCTG 
GAAGTAGCAA TATTAAGAGA TGATTTTCGT CAGGCACCCA ATGATGTGGT AGTAGCAGCA 
GGAGAACCAG CAGTCATGGA ATGCATTCCA CCCAGAGGTC ATCCTGAACC CACTATTTCC 
TGGAAAAAGG ATAACATGCG CATAAGTGAT AAAGATGAAC GCATCACAAT TCGAGGTGGG 
AAACTAATGA TTTCCAATAC GAGAAAGAGC GATGCAGGCA TGTACATCTG TGTGGCTACA 
AACATGGTGG GAGAACGAGA TAGCGAACCT GCTCAGCTTG TGGTCTTTGA ACGCCCCACA 
TTTATTAGAA GGCCCATAAA CCAAGTTGTT CTAGCAGATG ATATGGTGGA TTTTAACTGT 
GAAGTTCAGG GTGATCCTGT ACCTACTGCT CGCTGGCGTA AGGAAGAAGG AGAACTACCA 
CGGGGCCGGT ATGAAATCCT TAATGATAAT ACATTGCGGA TCTCTCGGGT GAGTGCTGAG 
GATGAAGGAA CTTACACTTG TATCACTGAA AATAGTGTTG GAAAATCAGA GGCTTCAGGA 
TCTCTTGGCG TTCATGTTGG CCCATTTTTT CCTCCACAAC TGGTCACCCG ACCACGAGAC 
CAAATAGTAG TTCAAGGACG TACTGTTACA TTCCAGTGTG AAACCAAAGG GAATCCACCA 
CCAGCTGTAT TTTGGCAGAA AGAAGGAAGT CAGATCCTTC TTTTCCCAAG CCAGCCTCCC 
CAAACAGTGG GACGATTTTC TGTCTCTCCG AGTGGAGAAC TGACCATAAT CGATGTTCAG 
ACAGGAGACT CAGGGTACTA CATGTGTCAA GCCATTAGTG TGGCTGGAAG TATCTTAGCT 
AAGGCTCTAC TTGAAGTGGA AGATGCAACA TCAGATCGAA TTCCTCCTAT AATTCGTAGA 
GGACCAGCCA ATCAAACCCT TGCTGTTGAC TCTACAGTGC AGATGCATTG CCAGGTATCT 
GGTAATCCTC TTCCAAGTAT ACATTGGCTC AAAGATGGCC AAAGGATTGT GGGAAGTGAC 
CCTCGAATAG GAGTGTTGGA CAGTGGAAAT TTACAGATCA CCCATTTACA GGTGACTGAT 
TCAGGTGTAT ATACATGTGT GGCTACAAGT TCAACTGGGG AGACTACATG GAGTGGATTT 
GTTGAAGTTC AAGAAAAAGG ATCTCCAATA CATGTGCAGC TTTCTGAGCC CAGCCTTGTT 
CCTGGATCTC CTTCCAAACC TCTTGTCACT GACGTTACCA AAAATAGTGT TACTTTAACA 
TGGCAACCCA ATCTGCAAGC AGGAAACACA CAAGTTAATT CCTATATCAT TGAAGCATTC 
AGCCAATCAG CAGGAAGCAC CTGGCAAACA GTTGCAGACA ACGTGAAAGT AGAAAAGCAC 
ACGGTGGTTG GGTTGAACCC AAATACAATT TATCTTTTCA TCATACGAGC AGTAAATTCA 
TATGGACTTA GTGACCCCAG CCCAATTTCA GAACCAGTGC GAACACAAGA TGTCAGTCCA 
ACGAGACAAG GTGTTGATCA TAGGCAAGTG CAGCGCCAAC TAGGGGAAGT GGTGGTTCAG 
CTTCAACAAC CGATTATTCT TACATCCTCT ACTGTGCAAG TTACATGGAC AGTGGATCGG 
CAAGCTCAGT TCATCCAAGG TTACCGGGTG ATGTATCGTC CAAAATCTAG TGTTTGGCTT 
GTACAGGATG TGAAGTCTCC CTCTGAACGG AATACTATCC TTGTGGACCT CAAAAAAGGC 
ACAGAATATG AAATCAAGAT CCGTCCTTAT TTTGATGAAT TTCAAGGCAT GGACAGTGAT 
GTGCTTTTGA TCCGTACACC TGAAGAAGTG CCAAGTGCCC CGCCTCAAGC AGTATCAGTT 
GTTACATTAG GAACGAATAA CAGCACAAAT ATTAGTATAT CGTGGGAACC TCCTATAGTG 
GAACATCAAA ATGGAATTAT TCAAGATTAT CGGATTTGGG TCTTGGGAAA TGAAACACGC 
TATCATATCA ACAGAACAGT AGATGGTACT GTCCACTCCA CTGTAATAAG AGGCCTTAGT 
CCTGGAGTTC TTTACCATGT GGAAGTGGCT GCTGCAACAA GTGCTGGTTT TGGTGTAAAA 
AGCCAACCAA TCACCATCCA GATAAAGCCT TCTTCAGATC AAGTTCCAGT TGCCAGTGGA 
GAAAATGTCA GCTTGACTGA ACAAATCACA GATGTTGTTA AACAGCCTGC ATTCATAGCT 
GGCATTGGAG GGGCATGCTG GGTAATTCTA ATGTGCTTTA GTGTCTGGAT CTACTGCCGA 
AGGAAGAAGA GGAAAGAACT GAGCCATTAT ACTGCTTCCT TTGCATACAC ACCAGCAGTA 
TCTTTTCCAG AAAGCTCAGG GCATATTACT GCAAGACCAG GAATGCTAGC TACAAGTGCA 
TCCAACTACC CTTGGCTAGC AGATTCATGG CCAGCAACCA ACTTACTACG AAACGGAAAG 
CCAAAAGAAC CTGCAGGGAA TTGTTGTCCA GGAAATCACA ATCCTTCAGA GAGATATTAT 
AATGATGCTG GCATTTCAAA CTATATTGCT CAGACTGAGA AGTTCGGAAC AGGTGTTTCA 
GATGGTCCCA TATACAGTAC AATTGATCCA ACCACTGATG ACATGCGAAC TTTTAATGGA 
GCTTTCTCTC AGCATACTAC ACCTTATGCA ACAACACCTG TTGTACCGTA TGGAGGACAA 
AACATGCATT CTCCTGAATT AGAAGAGAAC CAGTGGAATG TACAGGCTGG AACATCTGGT 
GTACTTTATG CACAACCAGA TCGAACTAAA GCAGAGAATG GTCCCAAATC TGGAAAGCAG 
AAGGCCATTG GAAAACCAGT AAAAACACCA TCACTAAATT GGACAGAAAT TCTTCCACCA 
CCCCCGCCTG CCAATGAGCT GAGTCAATAC CAAGAAGAAG ATACAGATGT AGAGATGAGC 
TCCGACACAG AAGAATGGTG TCCACCATTG CCTGAAAGAA CTTACCTCAT GGAAAACATT 
CAAGAAGGCT GCCCACTTCC TCCACCTCGG GGTGAGGCTT CTTCTCCTGC TCATTCTTAC 
GGTCAGCAGT CAACAGCCAC TCTAACACCA TCTCCCCAGG ATGAGATGCA TGCTGCAGAA 
GATATTCCAA GGCTACATCA GTTTGAGATA CCCCCTCATC CTAGACGACT GCCATCTGGA 
TCGGTACCAA TTCCAAGAGC TCCAAGTCCT CCTCTGACAC AATCAAATCC TAGTCTTCAT 
ACAGCAACGG AAGGTGCAGA AGCACAACGG CAGGGACACA GGCGGAACTT AAGCCAAAAT 
CCTAATCTAA GTGCTGAAAA TGTCAACTTG CCAAACAGCG GAAAGTCACA ACTAGGAGAA 
AATATAAGCA CACCTCCAGG ACAGAAATCG CGAGCAAAGA AGAAGTCAAA ATCAGGTCAC 
TATCGCAGGG AAGCAAAACA AGGAGATCTT CCTCCACCAC CTGAGCCCCC TCCAGCAGAT 
GAGGAACCAT GTTCTCCACA CACTAATGGA CTACGTATGG AATGTTCAGC CTCGTCCTTG 
GAAAGGGATA CAAGTACTTC TTCAGTTGAG CGGAGAAATG GGGTATTACA ACAGCGAGGA 
GCAGCAGGAC CTCAATTCCA TCAGAGAGAT GATGAAGAAC TAATTCCTTA CAGTAAACCT 
AACTTCCTAT CACGGAGTCA GATGTCAAGT AATTGTTCCA CAACAGGAAG CAGCTCCTCC 
AGAGGGTCAG CTGGATCTAG AGGTCAGGCA TCTGGAAGGA GCAAAACAGG AGACCGGAAA 
GAGGAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_012966981.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822435.2 Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X2, mRNA.

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CloneID	OXa61799
Clone ID Related Accession (Same CDS sequence)	XM_012966981.2
Accession Version	XM_012966981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ATCTGCTAAA GACTCTGCTG CAGATGAACC TCTTTGCGGA CTCCATCCCA 
GGCGAGAACT CCAATTCCAC TGATTTATTT TACCAGTTTA ACTCATCCTT TCCTGCTTTC 
AATGACAGCT TAATTGACTT CATCAACCTG ACCAACTTCA ATGGTTCTAG ACCCCGACTA 
GAAGATTTCC CGCCTCGAAT AGTGGAGCAT CCTTCTGATC ACATCGTCTC AAAGGGGGAA 
CCAGCTACTT TAAACTGCAA AGCAGAAGGC CGACCAACAC CTATTATTGA GTGGTACAAG 
GATGGGGAAA GGGTGGAGAC AGACAAAGAC GATCCTCGGT CACATCGCAT GCTGTTACCC 
AGTGGCTCAC TTTTCTTTCT ACGAATTGTT CATGGCCGTC GGAGTAAACC CGATGAAGGG 
GTATACATTT GTGTTGCAAG AAATTACCTT GGAGAGTCTG TCAGTCGAAA TGCATCTCTG 
GAAGTAGCAA TATTAAGAGA TGATTTTCGT CAGGCACCCA ATGATGTGGT AGTAGCAGCA 
GGAGAACCAG CAGTCATGGA ATGCATTCCA CCCAGAGGTC ATCCTGAACC CACTATTTCC 
TGGAAAAAGG ATAACATGCG CATAAGTGAT AAAGATGAAC GCATCACAAT TCGAGGTGGG 
AAACTAATGA TTTCCAATAC GAGAAAGAGC GATGCAGGCA TGTACATCTG TGTGGCTACA 
AACATGGTGG GAGAACGAGA TAGCGAACCT GCTCAGCTTG TGGTCTTTGA ACGCCCCACA 
TTTATTAGAA GGCCCATAAA CCAAGTTGTT CTAGCAGATG ATATGGTGGA TTTTAACTGT 
GAAGTTCAGG GTGATCCTGT ACCTACTGCT CGCTGGCGTA AGGAAGAAGG AGAACTACCA 
CGGGGCCGGT ATGAAATCCT TAATGATAAT ACATTGCGGA TCTCTCGGGT GAGTGCTGAG 
GATGAAGGAA CTTACACTTG TATCACTGAA AATAGTGTTG GAAAATCAGA GGCTTCAGGA 
TCTCTTGGCG TTCATGTTCC TCCACAACTG GTCACCCGAC CACGAGACCA AATAGTAGTT 
CAAGGACGTA CTGTTACATT CCAGTGTGAA ACCAAAGGGA ATCCACCACC AGCTGTATTT 
TGGCAGAAAG AAGGAAGTCA GATCCTTCTT TTCCCAAGCC AGCCTCCCCA AACAGTGGGA 
CGATTTTCTG TCTCTCCGAG TGGAGAACTG ACCATAATCG ATGTTCAGAC AGGAGACTCA 
GGGTACTACA TGTGTCAAGC CATTAGTGTG GCTGGAAGTA TCTTAGCTAA GGCTCTACTT 
GAAGTGGAAG ATGCAACATC AGATCGAATT CCTCCTATAA TTCGTAGAGG ACCAGCCAAT 
CAAACCCTTG CTGTTGACTC TACAGTGCAG ATGCATTGCC AGGTATCTGG TAATCCTCTT 
CCAAGTATAC ATTGGCTCAA AGATGGCCAA AGGATTGTGG GAAGTGACCC TCGAATAGGA 
GTGTTGGACA GTGGAAATTT ACAGATCACC CATTTACAGG TGACTGATTC AGGTGTATAT 
ACATGTGTGG CTACAAGTTC AACTGGGGAG ACTACATGGA GTGGATTTGT TGAAGTTCAA 
GAAAAAGGAT CTCCAATACA TGTGCAGCTT TCTGAGCCCA GCCTTGTTCC TGGATCTCCT 
TCCAAACCTC TTGTCACTGA CGTTACCAAA AATAGTGTTA CTTTAACATG GCAACCCAAT 
CTGCAAGCAG GAAACACACA AGTTAATTCC TATATCATTG AAGCATTCAG CCAATCAGCA 
GGAAGCACCT GGCAAACAGT TGCAGACAAC GTGAAAGTAG AAAAGCACAC GGTGGTTGGG 
TTGAACCCAA ATACAATTTA TCTTTTCATC ATACGAGCAG TAAATTCATA TGGACTTAGT 
GACCCCAGCC CAATTTCAGA ACCAGTGCGA ACACAAGATG TCAGTCCAAC GAGACAAGGT 
GTTGATCATA GGCAAGTGCA GCGCCAACTA GGGGAAGTGG TGGTTCAGCT TCAACAACCG 
ATTATTCTTA CATCCTCTAC TGTGCAAGTT ACATGGACAG TGGATCGGCA AGCTCAGTTC 
ATCCAAGGTT ACCGGGTGAT GTATCGTCCA AAATCTAGTG TTTGGCTTGT ACAGGATGTG 
AAGTCTCCCT CTGAACGGAA TACTATCCTT GTGGACCTCA AAAAAGGCAC AGAATATGAA 
ATCAAGATCC GTCCTTATTT TGATGAATTT CAAGGCATGG ACAGTGATGT GCTTTTGATC 
CGTACACCTG AAGAAGTGCC AAGTGCCCCG CCTCAAGCAG TATCAGTTGT TACATTAGGA 
ACGAATAACA GCACAAATAT TAGTATATCG TGGGAACCTC CTATAGTGGA ACATCAAAAT 
GGAATTATTC AAGATTATCG GATTTGGGTC TTGGGAAATG AAACACGCTA TCATATCAAC 
AGAACAGTAG ATGGTACTGT CCACTCCACT GTAATAAGAG GCCTTAGTCC TGGAGTTCTT 
TACCATGTGG AAGTGGCTGC TGCAACAAGT GCTGGTTTTG GTGTAAAAAG CCAACCAATC 
ACCATCCAGA TAAAGCCTTC TTCAGATCAA GTTCCAGTTG CCAGTGGAGA AAATGTCAGC 
TTGACTGAAC AAATCACAGA TGTTGTTAAA CAGCCTGCAT TCATAGCTGG CATTGGAGGG 
GCATGCTGGG TAATTCTAAT GTGCTTTAGT GTCTGGATCT ACTGCCGAAG GAAGAAGAGG 
AAAGAACTGA GCCATTATAC TGCTTCCTTT GCATACACAC CAGCAGTATC TTTTCCAGAA 
AGCTCAGGGC ATATTACTGC AAGACCAGGA ATGCTAGCTA CAAGTGCATC CAACTACCCT 
TGGCTAGCAG ATTCATGGCC AGCAACCAAC TTACTACGAA ACGGAAAGCC AAAAGAACCT 
GCAGGGAATT GTTGTCCAGG AAATCACAAT CCTTCAGAGA GATATTATAA TGATGCTGGC 
ATTTCAAACT ATATTGCTCA GACTGAGAAG TTCGGAACAG GTGTTTCAGA TGGTCCCATA 
TACAGTACAA TTGATCCAAC CACTGATGAC ATGCGAACTT TTAATGGAGC TTTCTCTCAG 
CATACTACAC CTTATGCAAC AACACCTGTT GTACCGTATG GAGGACAAAA CATGCATTCT 
CCTGAATTAG AAGAGAACCA GTGGAATGTA CAGGCTGGAA CATCTGGTGT ACTTTATGCA 
CAACCAGATC GAACTAAAGC AGAGAATGGT CCCAAATCTG GAAAGCAGAA GGCCATTGGA 
AAACCAGTAA AAACACCATC ACTAAATTGG ACAGAAATTC TTCCACCACC CCCGCCTGCC 
AATGAGCTGA GTCAATACCA AGAAGAAGAT ACAGATGTAG AGATGAGCTC CGACACAGAA 
GAATGGTGTC CACCATTGCC TGAAAGAACT TACCTCATGG AAAACATTCA AGAAGGCTGC 
CCACTTCCTC CACCTCGGGG TGAGGCTTCT TCTCCTGCTC ATTCTTACGG TCAGCAGTCA 
ACAGCCACTC TAACACCATC TCCCCAGGAT GAGATGCATG CTGCAGAAGA TATTCCAAGG 
CTACATCAGT TTGAGATACC CCCTCATCCT AGACGACTGC CATCTGGATC GGTACCAATT 
CCAAGAGCTC CAAGTCCTCC TCTGACACAA TCAAATCCTA GTCTTCATAC AGCAACGGAA 
GGTGCAGAAG CACAACGGCA GGGACACAGG CGGAACTTAA GCCAAAATCC TAATCTAAGT 
GCTGAAAATG TCAACTTGCC AAACAGCGGA AAGTCACAAC TAGGAGAAAA TATAAGCACA 
CCTCCAGGAC AGAAATCGCG AGCAAAGAAG AAGTCAAAAT CAGGTCACTA TCGCAGGGAA 
GCAAAACAAG GAGATCTTCC TCCACCACCT GAGCCCCCTC CAGCAGATGA GGAACCATGT 
TCTCCACACA CTAATGGACT ACGTATGGAA TGTTCAGCCT CGTCCTTGGA AAGGGATACA 
AGTACTTCTT CAGTTGAGCG GAGAAATGGG GTATTACAAC AGCGAGGAGC AGCAGGACCT 
CAATTCCATC AGAGAGATGA TGAAGAACTA ATTCCTTACA GTAAACCTAA CTTCCTATCA 
CGGAGTCAGA TGTCAAGTAA TTGTTCCACA ACAGGAAGCA GCTCCTCCAG AGGGTCAGCT 
GGATCTAGAG GTCAGGCATC TGGAAGGAGC AAAACAGGAG ACCGGAAAGA GGAAAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822435.2
CDS	362..4624
Translation	MLRYLLKTLLQMNLFADSIPGENSNSTDLFYQFNSSFPAFNDSLIDFINLTNFNGSRPRLEDFPPRIVEHPSDHIVSKGEPATLNCKAEGRPTPIIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYICVARNYLGESVSRNASLEVAILRDDFRQAPNDVVVAAGEPAVMECIPPRGHPEPTISWKKDNMRISDKDERITIRGGKLMISNTRKSDAGMYICVATNMVGERDSEPAQLVVFERPTFIRRPINQVVLADDMVDFNCEVQGDPVPTARWRKEEGELPRGRYEILNDNTLRISRVSAEDEGTYTCITENSVGKSEASGSLGVHVPPQLVTRPRDQIVVQGRTVTFQCETKGNPPPAVFWQKEGSQILLFPSQPPQTVGRFSVSPSGELTIIDVQTGDSGYYMCQAISVAGSILAKALLEVEDATSDRIPPIIRRGPANQTLAVDSTVQMHCQVSGNPLPSIHWLKDGQRIVGSDPRIGVLDSGNLQITHLQVTDSGVYTCVATSSTGETTWSGFVEVQEKGSPIHVQLSEPSLVPGSPSKPLVTDVTKNSVTLTWQPNLQAGNTQVNSYIIEAFSQSAGSTWQTVADNVKVEKHTVVGLNPNTIYLFIIRAVNSYGLSDPSPISEPVRTQDVSPTRQGVDHRQVQRQLGEVVVQLQQPIILTSSTVQVTWTVDRQAQFIQGYRVMYRPKSSVWLVQDVKSPSERNTILVDLKKGTEYEIKIRPYFDEFQGMDSDVLLIRTPEEVPSAPPQAVSVVTLGTNNSTNISISWEPPIVEHQNGIIQDYRIWVLGNETRYHINRTVDGTVHSTVIRGLSPGVLYHVEVAAATSAGFGVKSQPITIQIKPSSDQVPVASGENVSLTEQITDVVKQPAFIAGIGGACWVILMCFSVWIYCRRKKRKELSHYTASFAYTPAVSFPESSGHITARPGMLATSASNYPWLADSWPATNLLRNGKPKEPAGNCCPGNHNPSERYYNDAGISNYIAQTEKFGTGVSDGPIYSTIDPTTDDMRTFNGAFSQHTTPYATTPVVPYGGQNMHSPELEENQWNVQAGTSGVLYAQPDRTKAENGPKSGKQKAIGKPVKTPSLNWTEILPPPPPANELSQYQEEDTDVEMSSDTEEWCPPLPERTYLMENIQEGCPLPPPRGEASSPAHSYGQQSTATLTPSPQDEMHAAEDIPRLHQFEIPPHPRRLPSGSVPIPRAPSPPLTQSNPSLHTATEGAEAQRQGHRRNLSQNPNLSAENVNLPNSGKSQLGENISTPPGQKSRAKKKSKSGHYRREAKQGDLPPPPEPPPADEEPCSPHTNGLRMECSASSLERDTSTSSVERRNGVLQQRGAAGPQFHQRDDEELIPYSKPNFLSRSQMSSNCSTTGSSSSRGSAGSRGQASGRSKTGDRKEEKR

Target ORF information:

RefSeq Version	XM_012966981.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966981.2

ORF Insert Sequence:

ATGCTGCGCT ATCTGCTAAA GACTCTGCTG CAGATGAACC TCTTTGCGGA CTCCATCCCA 
GGCGAGAACT CCAATTCCAC TGATTTATTT TACCAGTTTA ACTCATCCTT TCCTGCTTTC 
AATGACAGCT TAATTGACTT CATCAACCTG ACCAACTTCA ATGGTTCTAG ACCCCGACTA 
GAAGATTTCC CGCCTCGAAT AGTGGAGCAT CCTTCTGATC ACATCGTCTC AAAGGGGGAA 
CCAGCTACTT TAAACTGCAA AGCAGAAGGC CGACCAACAC CTATTATTGA GTGGTACAAG 
GATGGGGAAA GGGTGGAGAC AGACAAAGAC GATCCTCGGT CACATCGCAT GCTGTTACCC 
AGTGGCTCAC TTTTCTTTCT ACGAATTGTT CATGGCCGTC GGAGTAAACC CGATGAAGGG 
GTATACATTT GTGTTGCAAG AAATTACCTT GGAGAGTCTG TCAGTCGAAA TGCATCTCTG 
GAAGTAGCAA TATTAAGAGA TGATTTTCGT CAGGCACCCA ATGATGTGGT AGTAGCAGCA 
GGAGAACCAG CAGTCATGGA ATGCATTCCA CCCAGAGGTC ATCCTGAACC CACTATTTCC 
TGGAAAAAGG ATAACATGCG CATAAGTGAT AAAGATGAAC GCATCACAAT TCGAGGTGGG 
AAACTAATGA TTTCCAATAC GAGAAAGAGC GATGCAGGCA TGTACATCTG TGTGGCTACA 
AACATGGTGG GAGAACGAGA TAGCGAACCT GCTCAGCTTG TGGTCTTTGA ACGCCCCACA 
TTTATTAGAA GGCCCATAAA CCAAGTTGTT CTAGCAGATG ATATGGTGGA TTTTAACTGT 
GAAGTTCAGG GTGATCCTGT ACCTACTGCT CGCTGGCGTA AGGAAGAAGG AGAACTACCA 
CGGGGCCGGT ATGAAATCCT TAATGATAAT ACATTGCGGA TCTCTCGGGT GAGTGCTGAG 
GATGAAGGAA CTTACACTTG TATCACTGAA AATAGTGTTG GAAAATCAGA GGCTTCAGGA 
TCTCTTGGCG TTCATGTTCC TCCACAACTG GTCACCCGAC CACGAGACCA AATAGTAGTT 
CAAGGACGTA CTGTTACATT CCAGTGTGAA ACCAAAGGGA ATCCACCACC AGCTGTATTT 
TGGCAGAAAG AAGGAAGTCA GATCCTTCTT TTCCCAAGCC AGCCTCCCCA AACAGTGGGA 
CGATTTTCTG TCTCTCCGAG TGGAGAACTG ACCATAATCG ATGTTCAGAC AGGAGACTCA 
GGGTACTACA TGTGTCAAGC CATTAGTGTG GCTGGAAGTA TCTTAGCTAA GGCTCTACTT 
GAAGTGGAAG ATGCAACATC AGATCGAATT CCTCCTATAA TTCGTAGAGG ACCAGCCAAT 
CAAACCCTTG CTGTTGACTC TACAGTGCAG ATGCATTGCC AGGTATCTGG TAATCCTCTT 
CCAAGTATAC ATTGGCTCAA AGATGGCCAA AGGATTGTGG GAAGTGACCC TCGAATAGGA 
GTGTTGGACA GTGGAAATTT ACAGATCACC CATTTACAGG TGACTGATTC AGGTGTATAT 
ACATGTGTGG CTACAAGTTC AACTGGGGAG ACTACATGGA GTGGATTTGT TGAAGTTCAA 
GAAAAAGGAT CTCCAATACA TGTGCAGCTT TCTGAGCCCA GCCTTGTTCC TGGATCTCCT 
TCCAAACCTC TTGTCACTGA CGTTACCAAA AATAGTGTTA CTTTAACATG GCAACCCAAT 
CTGCAAGCAG GAAACACACA AGTTAATTCC TATATCATTG AAGCATTCAG CCAATCAGCA 
GGAAGCACCT GGCAAACAGT TGCAGACAAC GTGAAAGTAG AAAAGCACAC GGTGGTTGGG 
TTGAACCCAA ATACAATTTA TCTTTTCATC ATACGAGCAG TAAATTCATA TGGACTTAGT 
GACCCCAGCC CAATTTCAGA ACCAGTGCGA ACACAAGATG TCAGTCCAAC GAGACAAGGT 
GTTGATCATA GGCAAGTGCA GCGCCAACTA GGGGAAGTGG TGGTTCAGCT TCAACAACCG 
ATTATTCTTA CATCCTCTAC TGTGCAAGTT ACATGGACAG TGGATCGGCA AGCTCAGTTC 
ATCCAAGGTT ACCGGGTGAT GTATCGTCCA AAATCTAGTG TTTGGCTTGT ACAGGATGTG 
AAGTCTCCCT CTGAACGGAA TACTATCCTT GTGGACCTCA AAAAAGGCAC AGAATATGAA 
ATCAAGATCC GTCCTTATTT TGATGAATTT CAAGGCATGG ACAGTGATGT GCTTTTGATC 
CGTACACCTG AAGAAGTGCC AAGTGCCCCG CCTCAAGCAG TATCAGTTGT TACATTAGGA 
ACGAATAACA GCACAAATAT TAGTATATCG TGGGAACCTC CTATAGTGGA ACATCAAAAT 
GGAATTATTC AAGATTATCG GATTTGGGTC TTGGGAAATG AAACACGCTA TCATATCAAC 
AGAACAGTAG ATGGTACTGT CCACTCCACT GTAATAAGAG GCCTTAGTCC TGGAGTTCTT 
TACCATGTGG AAGTGGCTGC TGCAACAAGT GCTGGTTTTG GTGTAAAAAG CCAACCAATC 
ACCATCCAGA TAAAGCCTTC TTCAGATCAA GTTCCAGTTG CCAGTGGAGA AAATGTCAGC 
TTGACTGAAC AAATCACAGA TGTTGTTAAA CAGCCTGCAT TCATAGCTGG CATTGGAGGG 
GCATGCTGGG TAATTCTAAT GTGCTTTAGT GTCTGGATCT ACTGCCGAAG GAAGAAGAGG 
AAAGAACTGA GCCATTATAC TGCTTCCTTT GCATACACAC CAGCAGTATC TTTTCCAGAA 
AGCTCAGGGC ATATTACTGC AAGACCAGGA ATGCTAGCTA CAAGTGCATC CAACTACCCT 
TGGCTAGCAG ATTCATGGCC AGCAACCAAC TTACTACGAA ACGGAAAGCC AAAAGAACCT 
GCAGGGAATT GTTGTCCAGG AAATCACAAT CCTTCAGAGA GATATTATAA TGATGCTGGC 
ATTTCAAACT ATATTGCTCA GACTGAGAAG TTCGGAACAG GTGTTTCAGA TGGTCCCATA 
TACAGTACAA TTGATCCAAC CACTGATGAC ATGCGAACTT TTAATGGAGC TTTCTCTCAG 
CATACTACAC CTTATGCAAC AACACCTGTT GTACCGTATG GAGGACAAAA CATGCATTCT 
CCTGAATTAG AAGAGAACCA GTGGAATGTA CAGGCTGGAA CATCTGGTGT ACTTTATGCA 
CAACCAGATC GAACTAAAGC AGAGAATGGT CCCAAATCTG GAAAGCAGAA GGCCATTGGA 
AAACCAGTAA AAACACCATC ACTAAATTGG ACAGAAATTC TTCCACCACC CCCGCCTGCC 
AATGAGCTGA GTCAATACCA AGAAGAAGAT ACAGATGTAG AGATGAGCTC CGACACAGAA 
GAATGGTGTC CACCATTGCC TGAAAGAACT TACCTCATGG AAAACATTCA AGAAGGCTGC 
CCACTTCCTC CACCTCGGGG TGAGGCTTCT TCTCCTGCTC ATTCTTACGG TCAGCAGTCA 
ACAGCCACTC TAACACCATC TCCCCAGGAT GAGATGCATG CTGCAGAAGA TATTCCAAGG 
CTACATCAGT TTGAGATACC CCCTCATCCT AGACGACTGC CATCTGGATC GGTACCAATT 
CCAAGAGCTC CAAGTCCTCC TCTGACACAA TCAAATCCTA GTCTTCATAC AGCAACGGAA 
GGTGCAGAAG CACAACGGCA GGGACACAGG CGGAACTTAA GCCAAAATCC TAATCTAAGT 
GCTGAAAATG TCAACTTGCC AAACAGCGGA AAGTCACAAC TAGGAGAAAA TATAAGCACA 
CCTCCAGGAC AGAAATCGCG AGCAAAGAAG AAGTCAAAAT CAGGTCACTA TCGCAGGGAA 
GCAAAACAAG GAGATCTTCC TCCACCACCT GAGCCCCCTC CAGCAGATGA GGAACCATGT 
TCTCCACACA CTAATGGACT ACGTATGGAA TGTTCAGCCT CGTCCTTGGA AAGGGATACA 
AGTACTTCTT CAGTTGAGCG GAGAAATGGG GTATTACAAC AGCGAGGAGC AGCAGGACCT 
CAATTCCATC AGAGAGATGA TGAAGAACTA ATTCCTTACA GTAAACCTAA CTTCCTATCA 
CGGAGTCAGA TGTCAAGTAA TTGTTCCACA ACAGGAAGCA GCTCCTCCAG AGGGTCAGCT 
GGATCTAGAG GTCAGGCATC TGGAAGGAGC AAAACAGGAG ACCGGAAAGA GGAAAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_012966982.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822436.2 Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X3, mRNA.

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CloneID	OXa61800
Clone ID Related Accession (Same CDS sequence)	XM_012966982.3
Accession Version	XM_012966982.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966982.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGC AGAAGATGAG CTTTATGGGC AAAACCTTCA CCTGCGTTTT ATTTGTGTCC 
GTATGTTCCG TCCTAGGTTC TAGACCCCGA CTAGAAGATT TCCCGCCTCG AATAGTGGAG 
CATCCTTCTG ATCACATCGT CTCAAAGGGG GAACCAGCTA CTTTAAACTG CAAAGCAGAA 
GGCCGACCAA CACCTATTAT TGAGTGGTAC AAGGATGGGG AAAGGGTGGA GACAGACAAA 
GACGATCCTC GGTCACATCG CATGCTGTTA CCCAGTGGCT CACTTTTCTT TCTACGAATT 
GTTCATGGCC GTCGGAGTAA ACCCGATGAA GGGGTATACA TTTGTGTTGC AAGAAATTAC 
CTTGGAGAGT CTGTCAGTCG AAATGCATCT CTGGAAGTAG CAATATTAAG AGATGATTTT 
CGTCAGGCAC CCAATGATGT GGTAGTAGCA GCAGGAGAAC CAGCAGTCAT GGAATGCATT 
CCACCCAGAG GTCATCCTGA ACCCACTATT TCCTGGAAAA AGGATAACAT GCGCATAAGT 
GATAAAGATG AACGCATCAC AATTCGAGGT GGGAAACTAA TGATTTCCAA TACGAGAAAG 
AGCGATGCAG GCATGTACAT CTGTGTGGCT ACAAACATGG TGGGAGAACG AGATAGCGAA 
CCTGCTCAGC TTGTGGTCTT TGAACGCCCC ACATTTATTA GAAGGCCCAT AAACCAAGTT 
GTTCTAGCAG ATGATATGGT GGATTTTAAC TGTGAAGTTC AGGGTGATCC TGTACCTACT 
GCTCGCTGGC GTAAGGAAGA AGGAGAACTA CCACGGGGCC GGTATGAAAT CCTTAATGAT 
AATACATTGC GGATCTCTCG GGTGAGTGCT GAGGATGAAG GAACTTACAC TTGTATCACT 
GAAAATAGTG TTGGAAAATC AGAGGCTTCA GGATCTCTTG GCGTTCATGT TGGCCCATTT 
TTTCCTCCAC AACTGGTCAC CCGACCACGA GACCAAATAG TAGTTCAAGG ACGTACTGTT 
ACATTCCAGT GTGAAACCAA AGGGAATCCA CCACCAGCTG TATTTTGGCA GAAAGAAGGA 
AGTCAGATCC TTCTTTTCCC AAGCCAGCCT CCCCAAACAG TGGGACGATT TTCTGTCTCT 
CCGAGTGGAG AACTGACCAT AATCGATGTT CAGACAGGAG ACTCAGGGTA CTACATGTGT 
CAAGCCATTA GTGTGGCTGG AAGTATCTTA GCTAAGGCTC TACTTGAAGT GGAAGATGCA 
ACATCAGATC GAATTCCTCC TATAATTCGT AGAGGACCAG CCAATCAAAC CCTTGCTGTT 
GACTCTACAG TGCAGATGCA TTGCCAGGTA TCTGGTAATC CTCTTCCAAG TATACATTGG 
CTCAAAGATG GCCAAAGGAT TGTGGGAAGT GACCCTCGAA TAGGAGTGTT GGACAGTGGA 
AATTTACAGA TCACCCATTT ACAGGTGACT GATTCAGGTG TATATACATG TGTGGCTACA 
AGTTCAACTG GGGAGACTAC ATGGAGTGGA TTTGTTGAAG TTCAAGAAAA AGGATCTCCA 
ATACATGTGC AGCTTTCTGA GCCCAGCCTT GTTCCTGGAT CTCCTTCCAA ACCTCTTGTC 
ACTGACGTTA CCAAAAATAG TGTTACTTTA ACATGGCAAC CCAATCTGCA AGCAGGAAAC 
ACACAAGTTA ATTCCTATAT CATTGAAGCA TTCAGCCAAT CAGCAGGAAG CACCTGGCAA 
ACAGTTGCAG ACAACGTGAA AGTAGAAAAG CACACGGTGG TTGGGTTGAA CCCAAATACA 
ATTTATCTTT TCATCATACG AGCAGTAAAT TCATATGGAC TTAGTGACCC CAGCCCAATT 
TCAGAACCAG TGCGAACACA AGATGTCAGT CCAACGAGAC AAGGTGTTGA TCATAGGCAA 
GTGCAGCGCC AACTAGGGGA AGTGGTGGTT CAGCTTCAAC AACCGATTAT TCTTACATCC 
TCTACTGTGC AAGTTACATG GACAGTGGAT CGGCAAGCTC AGTTCATCCA AGGTTACCGG 
GTGATGTATC GTCCAAAATC TAGTGTTTGG CTTGTACAGG ATGTGAAGTC TCCCTCTGAA 
CGGAATACTA TCCTTGTGGA CCTCAAAAAA GGCACAGAAT ATGAAATCAA GATCCGTCCT 
TATTTTGATG AATTTCAAGG CATGGACAGT GATGTGCTTT TGATCCGTAC ACCTGAAGAA 
GTGCCAAGTG CCCCGCCTCA AGCAGTATCA GTTGTTACAT TAGGAACGAA TAACAGCACA 
AATATTAGTA TATCGTGGGA ACCTCCTATA GTGGAACATC AAAATGGAAT TATTCAAGAT 
TATCGGATTT GGGTCTTGGG AAATGAAACA CGCTATCATA TCAACAGAAC AGTAGATGGT 
ACTGTCCACT CCACTGTAAT AAGAGGCCTT AGTCCTGGAG TTCTTTACCA TGTGGAAGTG 
GCTGCTGCAA CAAGTGCTGG TTTTGGTGTA AAAAGCCAAC CAATCACCAT CCAGATAAAG 
CCTTCTTCAG ATCAAGTTCC AGTTGCCAGT GGAGAAAATG TCAGCTTGAC TGAACAAATC 
ACAGATGTTG TTAAACAGCC TGCATTCATA GCTGGCATTG GAGGGGCATG CTGGGTAATT 
CTAATGTGCT TTAGTGTCTG GATCTACTGC CGAAGGAAGA AGAGGAAAGA ACTGAGCCAT 
TATACTGCTT CCTTTGCATA CACACCAGCA GTATCTTTTC CAGAAAGCTC AGGGCATATT 
ACTGCAAGAC CAGGAATGCT AGCTACAAGT GCATCCAACT ACCCTTGGCT AGCAGATTCA 
TGGCCAGCAA CCAACTTACT ACGAAACGGA AAGCCAAAAG AACCTGCAGG GAATTGTTGT 
CCAGGAAATC ACAATCCTTC AGAGAGATAT TATAATGATG CTGGCATTTC AAACTATATT 
GCTCAGACTG AGAAGTTCGG AACAGGTGTT TCAGATGGTC CCATATACAG TACAATTGAT 
CCAACCACTG ATGACATGCG AACTTTTAAT GGAGCTTTCT CTCAGCATAC TACACCTTAT 
GCAACAACAC CTGTTGTACC GTATGGAGGA CAAAACATGC ATTCTCCTGA ATTAGAAGAG 
AACCAGTGGA ATGTACAGGC TGGAACATCT GGTGTACTTT ATGCACAACC AGATCGAACT 
AAAGCAGAGA ATGGTCCCAA ATCTGGAAAG CAGAAGGCCA TTGGAAAACC AGTAAAAACA 
CCATCACTAA ATTGGACAGA AATTCTTCCA CCACCCCCGC CTGCCAATGA GCTGAGTCAA 
TACCAAGAAG AAGATACAGA TGTAGAGATG AGCTCCGACA CAGAAGAATG GTGTCCACCA 
TTGCCTGAAA GAACTTACCT CATGGAAAAC ATTCAAGAAG GCTGCCCACT TCCTCCACCT 
CGGGGTGAGG CTTCTTCTCC TGCTCATTCT TACGGTCAGC AGTCAACAGC CACTCTAACA 
CCATCTCCCC AGGATGAGAT GCATGCTGCA GAAGATATTC CAAGGCTACA TCAGTTTGAG 
ATACCCCCTC ATCCTAGACG ACTGCCATCT GGATCGGTAC CAATTCCAAG AGCTCCAAGT 
CCTCCTCTGA CACAATCAAA TCCTAGTCTT CATACAGCAA CGGAAGGTGC AGAAGCACAA 
CGGCAGGGAC ACAGGCGGAA CTTAAGCCAA AATCCTAATC TAAGTGCTGA AAATGTCAAC 
TTGCCAAACA GCGGAAAGTC ACAACTAGGA GAAAATATAA GCACACCTCC AGGACAGAAA 
TCGCGAGCAA AGAAGAAGTC AAAATCAGGT CACTATCGCA GGGAAGCAAA ACAAGGAGAT 
CTTCCTCCAC CACCTGAGCC CCCTCCAGCA GATGAGGAAC CATGTTCTCC ACACACTAAT 
GGACTACGTA TGGAATGTTC AGCCTCGTCC TTGGAAAGGG ATACAAGTAC TTCTTCAGTT 
GAGCGGAGAA ATGGGGTATT ACAACAGCGA GGAGCAGCAG GACCTCAATT CCATCAGAGA 
GATGATGAAG AACTAATTCC TTACAGTAAA CCTAACTTCC TATCACGGAG TCAGATGTCA 
AGTAATTGTT CCACAACAGG AAGCAGCTCC TCCAGAGGGT CAGCTGGATC TAGAGGTCAG 
GCATCTGGAA GGAGCAAAAC AGGAGACCGG AAAGAGGAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822436.2
CDS	497..4684
Translation	MERQKMSFMGKTFTCVLFVSVCSVLGSRPRLEDFPPRIVEHPSDHIVSKGEPATLNCKAEGRPTPIIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYICVARNYLGESVSRNASLEVAILRDDFRQAPNDVVVAAGEPAVMECIPPRGHPEPTISWKKDNMRISDKDERITIRGGKLMISNTRKSDAGMYICVATNMVGERDSEPAQLVVFERPTFIRRPINQVVLADDMVDFNCEVQGDPVPTARWRKEEGELPRGRYEILNDNTLRISRVSAEDEGTYTCITENSVGKSEASGSLGVHVGPFFPPQLVTRPRDQIVVQGRTVTFQCETKGNPPPAVFWQKEGSQILLFPSQPPQTVGRFSVSPSGELTIIDVQTGDSGYYMCQAISVAGSILAKALLEVEDATSDRIPPIIRRGPANQTLAVDSTVQMHCQVSGNPLPSIHWLKDGQRIVGSDPRIGVLDSGNLQITHLQVTDSGVYTCVATSSTGETTWSGFVEVQEKGSPIHVQLSEPSLVPGSPSKPLVTDVTKNSVTLTWQPNLQAGNTQVNSYIIEAFSQSAGSTWQTVADNVKVEKHTVVGLNPNTIYLFIIRAVNSYGLSDPSPISEPVRTQDVSPTRQGVDHRQVQRQLGEVVVQLQQPIILTSSTVQVTWTVDRQAQFIQGYRVMYRPKSSVWLVQDVKSPSERNTILVDLKKGTEYEIKIRPYFDEFQGMDSDVLLIRTPEEVPSAPPQAVSVVTLGTNNSTNISISWEPPIVEHQNGIIQDYRIWVLGNETRYHINRTVDGTVHSTVIRGLSPGVLYHVEVAAATSAGFGVKSQPITIQIKPSSDQVPVASGENVSLTEQITDVVKQPAFIAGIGGACWVILMCFSVWIYCRRKKRKELSHYTASFAYTPAVSFPESSGHITARPGMLATSASNYPWLADSWPATNLLRNGKPKEPAGNCCPGNHNPSERYYNDAGISNYIAQTEKFGTGVSDGPIYSTIDPTTDDMRTFNGAFSQHTTPYATTPVVPYGGQNMHSPELEENQWNVQAGTSGVLYAQPDRTKAENGPKSGKQKAIGKPVKTPSLNWTEILPPPPPANELSQYQEEDTDVEMSSDTEEWCPPLPERTYLMENIQEGCPLPPPRGEASSPAHSYGQQSTATLTPSPQDEMHAAEDIPRLHQFEIPPHPRRLPSGSVPIPRAPSPPLTQSNPSLHTATEGAEAQRQGHRRNLSQNPNLSAENVNLPNSGKSQLGENISTPPGQKSRAKKKSKSGHYRREAKQGDLPPPPEPPPADEEPCSPHTNGLRMECSASSLERDTSTSSVERRNGVLQQRGAAGPQFHQRDDEELIPYSKPNFLSRSQMSSNCSTTGSSSSRGSAGSRGQASGRSKTGDRKEEKR

Target ORF information:

RefSeq Version	XM_012966982.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966982.3

ORF Insert Sequence:

ATGGAAAGGC AGAAGATGAG CTTTATGGGC AAAACCTTCA CCTGCGTTTT ATTTGTGTCC 
GTATGTTCCG TCCTAGGTTC TAGACCCCGA CTAGAAGATT TCCCGCCTCG AATAGTGGAG 
CATCCTTCTG ATCACATCGT CTCAAAGGGG GAACCAGCTA CTTTAAACTG CAAAGCAGAA 
GGCCGACCAA CACCTATTAT TGAGTGGTAC AAGGATGGGG AAAGGGTGGA GACAGACAAA 
GACGATCCTC GGTCACATCG CATGCTGTTA CCCAGTGGCT CACTTTTCTT TCTACGAATT 
GTTCATGGCC GTCGGAGTAA ACCCGATGAA GGGGTATACA TTTGTGTTGC AAGAAATTAC 
CTTGGAGAGT CTGTCAGTCG AAATGCATCT CTGGAAGTAG CAATATTAAG AGATGATTTT 
CGTCAGGCAC CCAATGATGT GGTAGTAGCA GCAGGAGAAC CAGCAGTCAT GGAATGCATT 
CCACCCAGAG GTCATCCTGA ACCCACTATT TCCTGGAAAA AGGATAACAT GCGCATAAGT 
GATAAAGATG AACGCATCAC AATTCGAGGT GGGAAACTAA TGATTTCCAA TACGAGAAAG 
AGCGATGCAG GCATGTACAT CTGTGTGGCT ACAAACATGG TGGGAGAACG AGATAGCGAA 
CCTGCTCAGC TTGTGGTCTT TGAACGCCCC ACATTTATTA GAAGGCCCAT AAACCAAGTT 
GTTCTAGCAG ATGATATGGT GGATTTTAAC TGTGAAGTTC AGGGTGATCC TGTACCTACT 
GCTCGCTGGC GTAAGGAAGA AGGAGAACTA CCACGGGGCC GGTATGAAAT CCTTAATGAT 
AATACATTGC GGATCTCTCG GGTGAGTGCT GAGGATGAAG GAACTTACAC TTGTATCACT 
GAAAATAGTG TTGGAAAATC AGAGGCTTCA GGATCTCTTG GCGTTCATGT TGGCCCATTT 
TTTCCTCCAC AACTGGTCAC CCGACCACGA GACCAAATAG TAGTTCAAGG ACGTACTGTT 
ACATTCCAGT GTGAAACCAA AGGGAATCCA CCACCAGCTG TATTTTGGCA GAAAGAAGGA 
AGTCAGATCC TTCTTTTCCC AAGCCAGCCT CCCCAAACAG TGGGACGATT TTCTGTCTCT 
CCGAGTGGAG AACTGACCAT AATCGATGTT CAGACAGGAG ACTCAGGGTA CTACATGTGT 
CAAGCCATTA GTGTGGCTGG AAGTATCTTA GCTAAGGCTC TACTTGAAGT GGAAGATGCA 
ACATCAGATC GAATTCCTCC TATAATTCGT AGAGGACCAG CCAATCAAAC CCTTGCTGTT 
GACTCTACAG TGCAGATGCA TTGCCAGGTA TCTGGTAATC CTCTTCCAAG TATACATTGG 
CTCAAAGATG GCCAAAGGAT TGTGGGAAGT GACCCTCGAA TAGGAGTGTT GGACAGTGGA 
AATTTACAGA TCACCCATTT ACAGGTGACT GATTCAGGTG TATATACATG TGTGGCTACA 
AGTTCAACTG GGGAGACTAC ATGGAGTGGA TTTGTTGAAG TTCAAGAAAA AGGATCTCCA 
ATACATGTGC AGCTTTCTGA GCCCAGCCTT GTTCCTGGAT CTCCTTCCAA ACCTCTTGTC 
ACTGACGTTA CCAAAAATAG TGTTACTTTA ACATGGCAAC CCAATCTGCA AGCAGGAAAC 
ACACAAGTTA ATTCCTATAT CATTGAAGCA TTCAGCCAAT CAGCAGGAAG CACCTGGCAA 
ACAGTTGCAG ACAACGTGAA AGTAGAAAAG CACACGGTGG TTGGGTTGAA CCCAAATACA 
ATTTATCTTT TCATCATACG AGCAGTAAAT TCATATGGAC TTAGTGACCC CAGCCCAATT 
TCAGAACCAG TGCGAACACA AGATGTCAGT CCAACGAGAC AAGGTGTTGA TCATAGGCAA 
GTGCAGCGCC AACTAGGGGA AGTGGTGGTT CAGCTTCAAC AACCGATTAT TCTTACATCC 
TCTACTGTGC AAGTTACATG GACAGTGGAT CGGCAAGCTC AGTTCATCCA AGGTTACCGG 
GTGATGTATC GTCCAAAATC TAGTGTTTGG CTTGTACAGG ATGTGAAGTC TCCCTCTGAA 
CGGAATACTA TCCTTGTGGA CCTCAAAAAA GGCACAGAAT ATGAAATCAA GATCCGTCCT 
TATTTTGATG AATTTCAAGG CATGGACAGT GATGTGCTTT TGATCCGTAC ACCTGAAGAA 
GTGCCAAGTG CCCCGCCTCA AGCAGTATCA GTTGTTACAT TAGGAACGAA TAACAGCACA 
AATATTAGTA TATCGTGGGA ACCTCCTATA GTGGAACATC AAAATGGAAT TATTCAAGAT 
TATCGGATTT GGGTCTTGGG AAATGAAACA CGCTATCATA TCAACAGAAC AGTAGATGGT 
ACTGTCCACT CCACTGTAAT AAGAGGCCTT AGTCCTGGAG TTCTTTACCA TGTGGAAGTG 
GCTGCTGCAA CAAGTGCTGG TTTTGGTGTA AAAAGCCAAC CAATCACCAT CCAGATAAAG 
CCTTCTTCAG ATCAAGTTCC AGTTGCCAGT GGAGAAAATG TCAGCTTGAC TGAACAAATC 
ACAGATGTTG TTAAACAGCC TGCATTCATA GCTGGCATTG GAGGGGCATG CTGGGTAATT 
CTAATGTGCT TTAGTGTCTG GATCTACTGC CGAAGGAAGA AGAGGAAAGA ACTGAGCCAT 
TATACTGCTT CCTTTGCATA CACACCAGCA GTATCTTTTC CAGAAAGCTC AGGGCATATT 
ACTGCAAGAC CAGGAATGCT AGCTACAAGT GCATCCAACT ACCCTTGGCT AGCAGATTCA 
TGGCCAGCAA CCAACTTACT ACGAAACGGA AAGCCAAAAG AACCTGCAGG GAATTGTTGT 
CCAGGAAATC ACAATCCTTC AGAGAGATAT TATAATGATG CTGGCATTTC AAACTATATT 
GCTCAGACTG AGAAGTTCGG AACAGGTGTT TCAGATGGTC CCATATACAG TACAATTGAT 
CCAACCACTG ATGACATGCG AACTTTTAAT GGAGCTTTCT CTCAGCATAC TACACCTTAT 
GCAACAACAC CTGTTGTACC GTATGGAGGA CAAAACATGC ATTCTCCTGA ATTAGAAGAG 
AACCAGTGGA ATGTACAGGC TGGAACATCT GGTGTACTTT ATGCACAACC AGATCGAACT 
AAAGCAGAGA ATGGTCCCAA ATCTGGAAAG CAGAAGGCCA TTGGAAAACC AGTAAAAACA 
CCATCACTAA ATTGGACAGA AATTCTTCCA CCACCCCCGC CTGCCAATGA GCTGAGTCAA 
TACCAAGAAG AAGATACAGA TGTAGAGATG AGCTCCGACA CAGAAGAATG GTGTCCACCA 
TTGCCTGAAA GAACTTACCT CATGGAAAAC ATTCAAGAAG GCTGCCCACT TCCTCCACCT 
CGGGGTGAGG CTTCTTCTCC TGCTCATTCT TACGGTCAGC AGTCAACAGC CACTCTAACA 
CCATCTCCCC AGGATGAGAT GCATGCTGCA GAAGATATTC CAAGGCTACA TCAGTTTGAG 
ATACCCCCTC ATCCTAGACG ACTGCCATCT GGATCGGTAC CAATTCCAAG AGCTCCAAGT 
CCTCCTCTGA CACAATCAAA TCCTAGTCTT CATACAGCAA CGGAAGGTGC AGAAGCACAA 
CGGCAGGGAC ACAGGCGGAA CTTAAGCCAA AATCCTAATC TAAGTGCTGA AAATGTCAAC 
TTGCCAAACA GCGGAAAGTC ACAACTAGGA GAAAATATAA GCACACCTCC AGGACAGAAA 
TCGCGAGCAA AGAAGAAGTC AAAATCAGGT CACTATCGCA GGGAAGCAAA ACAAGGAGAT 
CTTCCTCCAC CACCTGAGCC CCCTCCAGCA GATGAGGAAC CATGTTCTCC ACACACTAAT 
GGACTACGTA TGGAATGTTC AGCCTCGTCC TTGGAAAGGG ATACAAGTAC TTCTTCAGTT 
GAGCGGAGAA ATGGGGTATT ACAACAGCGA GGAGCAGCAG GACCTCAATT CCATCAGAGA 
GATGATGAAG AACTAATTCC TTACAGTAAA CCTAACTTCC TATCACGGAG TCAGATGTCA 
AGTAATTGTT CCACAACAGG AAGCAGCTCC TCCAGAGGGT CAGCTGGATC TAGAGGTCAG 
GCATCTGGAA GGAGCAAAAC AGGAGACCGG AAAGAGGAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_002937521.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937567.2 Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X1, mRNA.

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CloneID	OXa13009
Clone ID Related Accession (Same CDS sequence)	XM_002937521.3
Accession Version	XM_002937521.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002937521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ATCTGCTAAA GACTCTGCTG CAGATGAACC TCTTTGCGGA CTCCATCCCA 
GGCGAGAACT CCAATTCCAC TGATTTATTT TACCAGTTTA ACTCATCCTT TCCTGCTTTC 
AATGACAGCT TAATTGACTT CATCAACCTG ACCAACTTCA ATGGTTCTAG ACCTCGACTA 
GAAGATTTCC CGCCTCGAAT AGTGGAGCAT CCTTCTGATC ACATTGTCTC AAAGGGGGAA 
CCAGCTACTT TAAACTGCAA AGCAGAAGGC CGACCAACAC CTATTATTGA GTGGTACAAG 
GATGGGGAAA GGGTGGAGAC AGACAAAGAC GATCCTCGGT CACATCGCAT GCTGTTACCC 
AGTGGCTCAC TTTTCTTTCT ACGAATTGTT CATGGCCGTC GGAGTAAACC CGATGAAGGG 
GTATACATTT GTGTTGCAAG AAATTACCTT GGAGAGTCTG TCAGTCGAAA TGCATCTCTG 
GAAGTAGCAA TATTAAGAGA TGATTTTCGT CAGGCACCCA ATGATGTGGT AGTAGCAGCA 
GGAGAACCAG CAGTCATGGA ATGCATTCCA CCCAGAGGTC ATCCTGAACC CACTATTTCC 
TGGAAAAAGG ATAACATGCG CATAAGTGAT AAAGATGAAC GCATCACAAT TCGAGGTGGG 
AAACTAATGA TTTCCAATAC GAGAAAGAGC GATGCAGGCA TGTACATCTG TGTGGCTACA 
AACATGGTGG GAGAACGAGA TAGCGAACCT GCTCAGCTTG TGGTCTTTGA ACGCCCCACA 
TTTATTAGAA GGCCCATAAA CCAAGTTGTT CTAGCAGATG ATATGGTGGA TTTTAACTGT 
GAAGTTCAGG GTGATCCTGT ACCTACTGCT CGCTGGCGTA AGGAAGAAGG AGAACTACCA 
CGGGGCCGGT ATGAAATCCT TAATGATAAT ACATTGCGGA TCTCTCGGGT GAGTGCTGAG 
GATGAAGGAA CTTACACTTG TATCACTGAA AATAGTGTTG GAAAATCAGA GGCTTCAGGA 
TCTCTTGGCG TTCATGTTGG CCCATTTTTT CCTCCACAAC TGGTCACCCG ACCACGAGAC 
CAAATAGTAG TTCAAGGACG TACTGTTACA TTCCAGTGTG AAACCAAAGG GAATCCACCA 
CCAGCTGTAT TTTGGCAGAA AGAAGGAAGT CAGATCCTTC TTTTCCCAAG CCAGCCTCCC 
CAAACAGTGG GACGATTTTC TGTCTCTCCG AGTGGAGAAC TGACCATAAT CGATGTTCAG 
ACAGGAGACT CAGGGTACTA CATGTGTCAA GCCATTAGTG TGGCTGGAAG TATCTTAGCT 
AAGGCTCTAC TTGAAGTGGA AGATGCAACA TCAGATCGAA TTCCTCCTAT AATTCGTAGA 
GGACCAGCCA ATCAAACCCT TGCTGTTGAC TCTACAGTGC AGATGCATTG CCAGGTATCT 
GGTAATCCTC TTCCAAGTAT ACATTGGCTC AAAGATGGCC AAAGGATTGT GGGAAGTGAC 
CCTCGAATAG GAGTGTTGGA CAGTGGAAAT TTACAGATCA CCCATTTACA GGTGACTGAT 
TCAGGTGTAT ATACATGTGT GGCTACAAGT TCAACTGGGG AGACTACATG GAGTGGATTT 
GTTGAAGTTC AAGAAAAAGG ATCTCCAATA CATGTGCAGC TTTCTGAGCC CAGCCTTGTT 
CCTGGATCTC CTTCCAAACC TCTTGTCACT GACGTTACCA AAAATAGTGT TACTTTAACA 
TGGCAACCCA ATCTGCAAGC AGGAAACACA CAAGTTAATT CCTATATCAT TGAAGCATTC 
AGCCAATCAG CAGGAAGCAC CTGGCAAACA GTTGCAGACA ACGTGAAAGT AGAAAAGCAC 
ACGGTGGTTG GGTTGAACCC AAATACAATT TATCTTTTCA TCATACGAGC AGTAAATTCA 
TATGGACTTA GTGACCCCAG CCCAATTTCA GAACCAGTGC GAACACAAGA TGTCAGTCCA 
ACGAGACAAG GTGTTGATCA TAGGCAAGTG CAGCGCCAAC TAGGGGAAGT GGTGGTTCAG 
CTTCAACAAC CGATTATTCT TACATCCTCT ACTGTGCAAG TTACATGGAC AGTGGATCGG 
CAAGCTCAGT TCATCCAAGG TTACCGGGTG ATGTATCGTC CAAAATCTAG TGTTTGGCTT 
GTACAGGATG TGAAGTCTCC CTCTGAACGG AATACTATCC TTGTGGACCT CAAAAAAGGC 
ACAGAATATG AAATCAAGAT CCGTCCTTAT TTTGATGAAT TTCAAGGCAT GGACAGTGAT 
GTGCTTTTGA TCCGTACACC TGAAGAAGTG CCAAGTGCCC CGCCTCAAGC AGTATCAGTT 
GTTACATTAG GAACGAATAA CAGCACAAAT ATTAGTATAT CGTGGGAACC TCCTATAGTG 
GAACATCAAA ATGGAATTAT TCAAGATTAT CGGATTTGGG TCTTGGGAAA TGAAACACGC 
TATCATATCA ACAGAACAGT AGATGGTACT GTCCACTCCA CTGTAATAAG AGGCCTCAGT 
CCTGGAGTTC TTTACCATGT GGAAGTGGCT GCTGCAACAA GTGCTGGTTT TGGTGTAAAA 
AGCCAACCAA TCACCATCCA GATAAAGCCT TCTTCAGATC AAGTTCCAGT TGCCAGTGGA 
GAAAATGTCA GCTTGACTGA ACAAATCACA GATGTTGTTA AACAGCCTGC ATTCATAGCT 
GGCATTGGAG GGGCATGCTG GGTAATTCTA ATGTGCTTTA GTGTCTGGAT CTACTGCCGA 
AGGAAGAAGA GGAAAGAACT GAGCCATTAT ACTGCTTCCT TTGCATACAC ACCAGCAGTA 
TCTTTTCCAG AAAGCTCAGG GCATATTACT GCAAGACCAG GAATGCTAGC TACAAGTGCA 
TCCAACTACC CTTGGCTAGC AGATTCATGG CCAGCAACCA ACTTACTACG AAACGGAAAG 
CCAAAAGAAC CTGCAGGGAA TTGTTGTCCA GGAAATCACA ATCCTTCAGA GAGATATTAT 
AATGATGCTG GCATTTCAAA CTATATTGCT CAGACTGAGA AGTTCGGAAC AGGTGTTTCA 
GATGGTCCCA TATACAGTAC AATTGATCCA ACCACTGATG ACATGCGAAC TTTTAATGGA 
GCTTTCTCTC AGCATACTAC ACCTTATGCA ACAACACCTG TTGTACCGTA TGGAGGACAA 
AACATGCATT CTCCTGAATT AGAAGAGAAC CAGTGGAATG TACAGGCTGG AACATCTGGT 
GTACTTTATG CACAACCAGA TCGAACTAAA GCAGAGAATG GTCCCAAATC TGGAAAGCAG 
AAGGCCATTG GAAAACCAGT AAAAACACCA TCACTAAATT GGACAGAAAT TCTTCCACCA 
CCCCCGCCTG CCAATGAGCT GAGTCAATAC CAAGAAGAAG ATACAGATAT AGAGATGAGC 
TCCGACACAG AAGAATGGTG TCCACCATTG CCTGAAAGAA CTTACCTCAT GGAAAACATT 
CAAGAAGGCT GCCCACTTCC TCCACCTCGG GGTGAGGCTT CTTCTCCTGC TCATTCTTAC 
GGTCAGCAGT CAACAGCCAC TCTAACACCA TCTCCCCAGG ATGAGATGCA TGCTGCAGAA 
GATATTCCAA GGCTACATCA GTTTGAGATA CCCCCTCATC CTAGACGACT GCCATCTGGA 
TCGGTACCAA TTCCAAGAGC TCCAAGTCCT CCTCTGACAC AATCAAATCC TAGTCTTCAT 
ACAGCAACGG AAGGTGCAGA AGCACAACGG CAGGGACACA GGCGGAACTT AAGCCAAAAT 
CCTAATCTAA GTGCTGAAAA TGTCAACTTG CCAAACAGCG GAAAGTCACA ACTAGGAGAA 
AATATAAGCA CACCTCCAGG ACAGAAATCG CGAGCAAAGA AGAAGTCAAA ATCAGGTCAC 
TATCGCAGGG AAGCAAAACA AGGAGATCTT CCTCCACCAC CTGAGCCCCC TCCAGCAGAT 
GAGGAACCAT GTTCTCCACA CACTAATGGA CTACGTATGG AATGTTCAGC CTCGTCCTTG 
GAAAGGGATA CAAGTACTTC TTCAGTTGAG CGGAGAAATG GGGTATTACA ACAGCGAGGA 
GCAGCAGGAC CTCAATTCCA TCAGAGAGAT GATGAAGAAC TAATTCCTTA CAGTAAACCT 
AACTTCCTAT CACGGAGTCA GATGTCAAGT AATTGTTCCA CAACAGGAAG CAGCTCCTCC 
AGAGGGTCAG CTGGATCTAG AGGTCAGGCA TCTGGAAGGA GCAAAACAGG AGACCGGAAA 
GAGGAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937567.2
CDS	362..4636
Translation	MLRYLLKTLLQMNLFADSIPGENSNSTDLFYQFNSSFPAFNDSLIDFINLTNFNGSRPRLEDFPPRIVEHPSDHIVSKGEPATLNCKAEGRPTPIIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYICVARNYLGESVSRNASLEVAILRDDFRQAPNDVVVAAGEPAVMECIPPRGHPEPTISWKKDNMRISDKDERITIRGGKLMISNTRKSDAGMYICVATNMVGERDSEPAQLVVFERPTFIRRPINQVVLADDMVDFNCEVQGDPVPTARWRKEEGELPRGRYEILNDNTLRISRVSAEDEGTYTCITENSVGKSEASGSLGVHVGPFFPPQLVTRPRDQIVVQGRTVTFQCETKGNPPPAVFWQKEGSQILLFPSQPPQTVGRFSVSPSGELTIIDVQTGDSGYYMCQAISVAGSILAKALLEVEDATSDRIPPIIRRGPANQTLAVDSTVQMHCQVSGNPLPSIHWLKDGQRIVGSDPRIGVLDSGNLQITHLQVTDSGVYTCVATSSTGETTWSGFVEVQEKGSPIHVQLSEPSLVPGSPSKPLVTDVTKNSVTLTWQPNLQAGNTQVNSYIIEAFSQSAGSTWQTVADNVKVEKHTVVGLNPNTIYLFIIRAVNSYGLSDPSPISEPVRTQDVSPTRQGVDHRQVQRQLGEVVVQLQQPIILTSSTVQVTWTVDRQAQFIQGYRVMYRPKSSVWLVQDVKSPSERNTILVDLKKGTEYEIKIRPYFDEFQGMDSDVLLIRTPEEVPSAPPQAVSVVTLGTNNSTNISISWEPPIVEHQNGIIQDYRIWVLGNETRYHINRTVDGTVHSTVIRGLSPGVLYHVEVAAATSAGFGVKSQPITIQIKPSSDQVPVASGENVSLTEQITDVVKQPAFIAGIGGACWVILMCFSVWIYCRRKKRKELSHYTASFAYTPAVSFPESSGHITARPGMLATSASNYPWLADSWPATNLLRNGKPKEPAGNCCPGNHNPSERYYNDAGISNYIAQTEKFGTGVSDGPIYSTIDPTTDDMRTFNGAFSQHTTPYATTPVVPYGGQNMHSPELEENQWNVQAGTSGVLYAQPDRTKAENGPKSGKQKAIGKPVKTPSLNWTEILPPPPPANELSQYQEEDTDIEMSSDTEEWCPPLPERTYLMENIQEGCPLPPPRGEASSPAHSYGQQSTATLTPSPQDEMHAAEDIPRLHQFEIPPHPRRLPSGSVPIPRAPSPPLTQSNPSLHTATEGAEAQRQGHRRNLSQNPNLSAENVNLPNSGKSQLGENISTPPGQKSRAKKKSKSGHYRREAKQGDLPPPPEPPPADEEPCSPHTNGLRMECSASSLERDTSTSSVERRNGVLQQRGAAGPQFHQRDDEELIPYSKPNFLSRSQMSSNCSTTGSSSSRGSAGSRGQASGRSKTGDRKEEKR

Target ORF information:

RefSeq Version	XM_002937521.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937521.3

ORF Insert Sequence:

ATGCTGCGCT ATCTGCTAAA GACTCTGCTG CAGATGAACC TCTTTGCGGA CTCCATCCCA 
GGCGAGAACT CCAATTCCAC TGATTTATTT TACCAGTTTA ACTCATCCTT TCCTGCTTTC 
AATGACAGCT TAATTGACTT CATCAACCTG ACCAACTTCA ATGGTTCTAG ACCTCGACTA 
GAAGATTTCC CGCCTCGAAT AGTGGAGCAT CCTTCTGATC ACATTGTCTC AAAGGGGGAA 
CCAGCTACTT TAAACTGCAA AGCAGAAGGC CGACCAACAC CTATTATTGA GTGGTACAAG 
GATGGGGAAA GGGTGGAGAC AGACAAAGAC GATCCTCGGT CACATCGCAT GCTGTTACCC 
AGTGGCTCAC TTTTCTTTCT ACGAATTGTT CATGGCCGTC GGAGTAAACC CGATGAAGGG 
GTATACATTT GTGTTGCAAG AAATTACCTT GGAGAGTCTG TCAGTCGAAA TGCATCTCTG 
GAAGTAGCAA TATTAAGAGA TGATTTTCGT CAGGCACCCA ATGATGTGGT AGTAGCAGCA 
GGAGAACCAG CAGTCATGGA ATGCATTCCA CCCAGAGGTC ATCCTGAACC CACTATTTCC 
TGGAAAAAGG ATAACATGCG CATAAGTGAT AAAGATGAAC GCATCACAAT TCGAGGTGGG 
AAACTAATGA TTTCCAATAC GAGAAAGAGC GATGCAGGCA TGTACATCTG TGTGGCTACA 
AACATGGTGG GAGAACGAGA TAGCGAACCT GCTCAGCTTG TGGTCTTTGA ACGCCCCACA 
TTTATTAGAA GGCCCATAAA CCAAGTTGTT CTAGCAGATG ATATGGTGGA TTTTAACTGT 
GAAGTTCAGG GTGATCCTGT ACCTACTGCT CGCTGGCGTA AGGAAGAAGG AGAACTACCA 
CGGGGCCGGT ATGAAATCCT TAATGATAAT ACATTGCGGA TCTCTCGGGT GAGTGCTGAG 
GATGAAGGAA CTTACACTTG TATCACTGAA AATAGTGTTG GAAAATCAGA GGCTTCAGGA 
TCTCTTGGCG TTCATGTTGG CCCATTTTTT CCTCCACAAC TGGTCACCCG ACCACGAGAC 
CAAATAGTAG TTCAAGGACG TACTGTTACA TTCCAGTGTG AAACCAAAGG GAATCCACCA 
CCAGCTGTAT TTTGGCAGAA AGAAGGAAGT CAGATCCTTC TTTTCCCAAG CCAGCCTCCC 
CAAACAGTGG GACGATTTTC TGTCTCTCCG AGTGGAGAAC TGACCATAAT CGATGTTCAG 
ACAGGAGACT CAGGGTACTA CATGTGTCAA GCCATTAGTG TGGCTGGAAG TATCTTAGCT 
AAGGCTCTAC TTGAAGTGGA AGATGCAACA TCAGATCGAA TTCCTCCTAT AATTCGTAGA 
GGACCAGCCA ATCAAACCCT TGCTGTTGAC TCTACAGTGC AGATGCATTG CCAGGTATCT 
GGTAATCCTC TTCCAAGTAT ACATTGGCTC AAAGATGGCC AAAGGATTGT GGGAAGTGAC 
CCTCGAATAG GAGTGTTGGA CAGTGGAAAT TTACAGATCA CCCATTTACA GGTGACTGAT 
TCAGGTGTAT ATACATGTGT GGCTACAAGT TCAACTGGGG AGACTACATG GAGTGGATTT 
GTTGAAGTTC AAGAAAAAGG ATCTCCAATA CATGTGCAGC TTTCTGAGCC CAGCCTTGTT 
CCTGGATCTC CTTCCAAACC TCTTGTCACT GACGTTACCA AAAATAGTGT TACTTTAACA 
TGGCAACCCA ATCTGCAAGC AGGAAACACA CAAGTTAATT CCTATATCAT TGAAGCATTC 
AGCCAATCAG CAGGAAGCAC CTGGCAAACA GTTGCAGACA ACGTGAAAGT AGAAAAGCAC 
ACGGTGGTTG GGTTGAACCC AAATACAATT TATCTTTTCA TCATACGAGC AGTAAATTCA 
TATGGACTTA GTGACCCCAG CCCAATTTCA GAACCAGTGC GAACACAAGA TGTCAGTCCA 
ACGAGACAAG GTGTTGATCA TAGGCAAGTG CAGCGCCAAC TAGGGGAAGT GGTGGTTCAG 
CTTCAACAAC CGATTATTCT TACATCCTCT ACTGTGCAAG TTACATGGAC AGTGGATCGG 
CAAGCTCAGT TCATCCAAGG TTACCGGGTG ATGTATCGTC CAAAATCTAG TGTTTGGCTT 
GTACAGGATG TGAAGTCTCC CTCTGAACGG AATACTATCC TTGTGGACCT CAAAAAAGGC 
ACAGAATATG AAATCAAGAT CCGTCCTTAT TTTGATGAAT TTCAAGGCAT GGACAGTGAT 
GTGCTTTTGA TCCGTACACC TGAAGAAGTG CCAAGTGCCC CGCCTCAAGC AGTATCAGTT 
GTTACATTAG GAACGAATAA CAGCACAAAT ATTAGTATAT CGTGGGAACC TCCTATAGTG 
GAACATCAAA ATGGAATTAT TCAAGATTAT CGGATTTGGG TCTTGGGAAA TGAAACACGC 
TATCATATCA ACAGAACAGT AGATGGTACT GTCCACTCCA CTGTAATAAG AGGCCTCAGT 
CCTGGAGTTC TTTACCATGT GGAAGTGGCT GCTGCAACAA GTGCTGGTTT TGGTGTAAAA 
AGCCAACCAA TCACCATCCA GATAAAGCCT TCTTCAGATC AAGTTCCAGT TGCCAGTGGA 
GAAAATGTCA GCTTGACTGA ACAAATCACA GATGTTGTTA AACAGCCTGC ATTCATAGCT 
GGCATTGGAG GGGCATGCTG GGTAATTCTA ATGTGCTTTA GTGTCTGGAT CTACTGCCGA 
AGGAAGAAGA GGAAAGAACT GAGCCATTAT ACTGCTTCCT TTGCATACAC ACCAGCAGTA 
TCTTTTCCAG AAAGCTCAGG GCATATTACT GCAAGACCAG GAATGCTAGC TACAAGTGCA 
TCCAACTACC CTTGGCTAGC AGATTCATGG CCAGCAACCA ACTTACTACG AAACGGAAAG 
CCAAAAGAAC CTGCAGGGAA TTGTTGTCCA GGAAATCACA ATCCTTCAGA GAGATATTAT 
AATGATGCTG GCATTTCAAA CTATATTGCT CAGACTGAGA AGTTCGGAAC AGGTGTTTCA 
GATGGTCCCA TATACAGTAC AATTGATCCA ACCACTGATG ACATGCGAAC TTTTAATGGA 
GCTTTCTCTC AGCATACTAC ACCTTATGCA ACAACACCTG TTGTACCGTA TGGAGGACAA 
AACATGCATT CTCCTGAATT AGAAGAGAAC CAGTGGAATG TACAGGCTGG AACATCTGGT 
GTACTTTATG CACAACCAGA TCGAACTAAA GCAGAGAATG GTCCCAAATC TGGAAAGCAG 
AAGGCCATTG GAAAACCAGT AAAAACACCA TCACTAAATT GGACAGAAAT TCTTCCACCA 
CCCCCGCCTG CCAATGAGCT GAGTCAATAC CAAGAAGAAG ATACAGATAT AGAGATGAGC 
TCCGACACAG AAGAATGGTG TCCACCATTG CCTGAAAGAA CTTACCTCAT GGAAAACATT 
CAAGAAGGCT GCCCACTTCC TCCACCTCGG GGTGAGGCTT CTTCTCCTGC TCATTCTTAC 
GGTCAGCAGT CAACAGCCAC TCTAACACCA TCTCCCCAGG ATGAGATGCA TGCTGCAGAA 
GATATTCCAA GGCTACATCA GTTTGAGATA CCCCCTCATC CTAGACGACT GCCATCTGGA 
TCGGTACCAA TTCCAAGAGC TCCAAGTCCT CCTCTGACAC AATCAAATCC TAGTCTTCAT 
ACAGCAACGG AAGGTGCAGA AGCACAACGG CAGGGACACA GGCGGAACTT AAGCCAAAAT 
CCTAATCTAA GTGCTGAAAA TGTCAACTTG CCAAACAGCG GAAAGTCACA ACTAGGAGAA 
AATATAAGCA CACCTCCAGG ACAGAAATCG CGAGCAAAGA AGAAGTCAAA ATCAGGTCAC 
TATCGCAGGG AAGCAAAACA AGGAGATCTT CCTCCACCAC CTGAGCCCCC TCCAGCAGAT 
GAGGAACCAT GTTCTCCACA CACTAATGGA CTACGTATGG AATGTTCAGC CTCGTCCTTG 
GAAAGGGATA CAAGTACTTC TTCAGTTGAG CGGAGAAATG GGGTATTACA ACAGCGAGGA 
GCAGCAGGAC CTCAATTCCA TCAGAGAGAT GATGAAGAAC TAATTCCTTA CAGTAAACCT 
AACTTCCTAT CACGGAGTCA GATGTCAAGT AATTGTTCCA CAACAGGAAG CAGCTCCTCC 
AGAGGGTCAG CTGGATCTAG AGGTCAGGCA TCTGGAAGGA GCAAAACAGG AGACCGGAAA 
GAGGAAAAGA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_012966981.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822435.1 Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa35708
Clone ID Related Accession (Same CDS sequence)	XM_012966981.1
Accession Version	XM_012966981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCT ATCTGCTAAA GACTCTGCTG CAGATGAACC TCTTTGCGGA CTCCATCCCA 
GGCGAGAACT CCAATTCCAC TGATTTATTT TACCAGTTTA ACTCATCCTT TCCTGCTTTC 
AATGACAGCT TAATTGACTT CATCAACCTG ACCAACTTCA ATGGTTCTAG ACCTCGACTA 
GAAGATTTCC CGCCTCGAAT AGTGGAGCAT CCTTCTGATC ACATTGTCTC AAAGGGGGAA 
CCAGCTACTT TAAACTGCAA AGCAGAAGGC CGACCAACAC CTATTATTGA GTGGTACAAG 
GATGGGGAAA GGGTGGAGAC AGACAAAGAC GATCCTCGGT CACATCGCAT GCTGTTACCC 
AGTGGCTCAC TTTTCTTTCT ACGAATTGTT CATGGCCGTC GGAGTAAACC CGATGAAGGG 
GTATACATTT GTGTTGCAAG AAATTACCTT GGAGAGTCTG TCAGTCGAAA TGCATCTCTG 
GAAGTAGCAA TATTAAGAGA TGATTTTCGT CAGGCACCCA ATGATGTGGT AGTAGCAGCA 
GGAGAACCAG CAGTCATGGA ATGCATTCCA CCCAGAGGTC ATCCTGAACC CACTATTTCC 
TGGAAAAAGG ATAACATGCG CATAAGTGAT AAAGATGAAC GCATCACAAT TCGAGGTGGG 
AAACTAATGA TTTCCAATAC GAGAAAGAGC GATGCAGGCA TGTACATCTG TGTGGCTACA 
AACATGGTGG GAGAACGAGA TAGCGAACCT GCTCAGCTTG TGGTCTTTGA ACGCCCCACA 
TTTATTAGAA GGCCCATAAA CCAAGTTGTT CTAGCAGATG ATATGGTGGA TTTTAACTGT 
GAAGTTCAGG GTGATCCTGT ACCTACTGCT CGCTGGCGTA AGGAAGAAGG AGAACTACCA 
CGGGGCCGGT ATGAAATCCT TAATGATAAT ACATTGCGGA TCTCTCGGGT GAGTGCTGAG 
GATGAAGGAA CTTACACTTG TATCACTGAA AATAGTGTTG GAAAATCAGA GGCTTCAGGA 
TCTCTTGGCG TTCATGTTCC TCCACAACTG GTCACCCGAC CACGAGACCA AATAGTAGTT 
CAAGGACGTA CTGTTACATT CCAGTGTGAA ACCAAAGGGA ATCCACCACC AGCTGTATTT 
TGGCAGAAAG AAGGAAGTCA GATCCTTCTT TTCCCAAGCC AGCCTCCCCA AACAGTGGGA 
CGATTTTCTG TCTCTCCGAG TGGAGAACTG ACCATAATCG ATGTTCAGAC AGGAGACTCA 
GGGTACTACA TGTGTCAAGC CATTAGTGTG GCTGGAAGTA TCTTAGCTAA GGCTCTACTT 
GAAGTGGAAG ATGCAACATC AGATCGAATT CCTCCTATAA TTCGTAGAGG ACCAGCCAAT 
CAAACCCTTG CTGTTGACTC TACAGTGCAG ATGCATTGCC AGGTATCTGG TAATCCTCTT 
CCAAGTATAC ATTGGCTCAA AGATGGCCAA AGGATTGTGG GAAGTGACCC TCGAATAGGA 
GTGTTGGACA GTGGAAATTT ACAGATCACC CATTTACAGG TGACTGATTC AGGTGTATAT 
ACATGTGTGG CTACAAGTTC AACTGGGGAG ACTACATGGA GTGGATTTGT TGAAGTTCAA 
GAAAAAGGAT CTCCAATACA TGTGCAGCTT TCTGAGCCCA GCCTTGTTCC TGGATCTCCT 
TCCAAACCTC TTGTCACTGA CGTTACCAAA AATAGTGTTA CTTTAACATG GCAACCCAAT 
CTGCAAGCAG GAAACACACA AGTTAATTCC TATATCATTG AAGCATTCAG CCAATCAGCA 
GGAAGCACCT GGCAAACAGT TGCAGACAAC GTGAAAGTAG AAAAGCACAC GGTGGTTGGG 
TTGAACCCAA ATACAATTTA TCTTTTCATC ATACGAGCAG TAAATTCATA TGGACTTAGT 
GACCCCAGCC CAATTTCAGA ACCAGTGCGA ACACAAGATG TCAGTCCAAC GAGACAAGGT 
GTTGATCATA GGCAAGTGCA GCGCCAACTA GGGGAAGTGG TGGTTCAGCT TCAACAACCG 
ATTATTCTTA CATCCTCTAC TGTGCAAGTT ACATGGACAG TGGATCGGCA AGCTCAGTTC 
ATCCAAGGTT ACCGGGTGAT GTATCGTCCA AAATCTAGTG TTTGGCTTGT ACAGGATGTG 
AAGTCTCCCT CTGAACGGAA TACTATCCTT GTGGACCTCA AAAAAGGCAC AGAATATGAA 
ATCAAGATCC GTCCTTATTT TGATGAATTT CAAGGCATGG ACAGTGATGT GCTTTTGATC 
CGTACACCTG AAGAAGTGCC AAGTGCCCCG CCTCAAGCAG TATCAGTTGT TACATTAGGA 
ACGAATAACA GCACAAATAT TAGTATATCG TGGGAACCTC CTATAGTGGA ACATCAAAAT 
GGAATTATTC AAGATTATCG GATTTGGGTC TTGGGAAATG AAACACGCTA TCATATCAAC 
AGAACAGTAG ATGGTACTGT CCACTCCACT GTAATAAGAG GCCTCAGTCC TGGAGTTCTT 
TACCATGTGG AAGTGGCTGC TGCAACAAGT GCTGGTTTTG GTGTAAAAAG CCAACCAATC 
ACCATCCAGA TAAAGCCTTC TTCAGATCAA GTTCCAGTTG CCAGTGGAGA AAATGTCAGC 
TTGACTGAAC AAATCACAGA TGTTGTTAAA CAGCCTGCAT TCATAGCTGG CATTGGAGGG 
GCATGCTGGG TAATTCTAAT GTGCTTTAGT GTCTGGATCT ACTGCCGAAG GAAGAAGAGG 
AAAGAACTGA GCCATTATAC TGCTTCCTTT GCATACACAC CAGCAGTATC TTTTCCAGAA 
AGCTCAGGGC ATATTACTGC AAGACCAGGA ATGCTAGCTA CAAGTGCATC CAACTACCCT 
TGGCTAGCAG ATTCATGGCC AGCAACCAAC TTACTACGAA ACGGAAAGCC AAAAGAACCT 
GCAGGGAATT GTTGTCCAGG AAATCACAAT CCTTCAGAGA GATATTATAA TGATGCTGGC 
ATTTCAAACT ATATTGCTCA GACTGAGAAG TTCGGAACAG GTGTTTCAGA TGGTCCCATA 
TACAGTACAA TTGATCCAAC CACTGATGAC ATGCGAACTT TTAATGGAGC TTTCTCTCAG 
CATACTACAC CTTATGCAAC AACACCTGTT GTACCGTATG GAGGACAAAA CATGCATTCT 
CCTGAATTAG AAGAGAACCA GTGGAATGTA CAGGCTGGAA CATCTGGTGT ACTTTATGCA 
CAACCAGATC GAACTAAAGC AGAGAATGGT CCCAAATCTG GAAAGCAGAA GGCCATTGGA 
AAACCAGTAA AAACACCATC ACTAAATTGG ACAGAAATTC TTCCACCACC CCCGCCTGCC 
AATGAGCTGA GTCAATACCA AGAAGAAGAT ACAGATATAG AGATGAGCTC CGACACAGAA 
GAATGGTGTC CACCATTGCC TGAAAGAACT TACCTCATGG AAAACATTCA AGAAGGCTGC 
CCACTTCCTC CACCTCGGGG TGAGGCTTCT TCTCCTGCTC ATTCTTACGG TCAGCAGTCA 
ACAGCCACTC TAACACCATC TCCCCAGGAT GAGATGCATG CTGCAGAAGA TATTCCAAGG 
CTACATCAGT TTGAGATACC CCCTCATCCT AGACGACTGC CATCTGGATC GGTACCAATT 
CCAAGAGCTC CAAGTCCTCC TCTGACACAA TCAAATCCTA GTCTTCATAC AGCAACGGAA 
GGTGCAGAAG CACAACGGCA GGGACACAGG CGGAACTTAA GCCAAAATCC TAATCTAAGT 
GCTGAAAATG TCAACTTGCC AAACAGCGGA AAGTCACAAC TAGGAGAAAA TATAAGCACA 
CCTCCAGGAC AGAAATCGCG AGCAAAGAAG AAGTCAAAAT CAGGTCACTA TCGCAGGGAA 
GCAAAACAAG GAGATCTTCC TCCACCACCT GAGCCCCCTC CAGCAGATGA GGAACCATGT 
TCTCCACACA CTAATGGACT ACGTATGGAA TGTTCAGCCT CGTCCTTGGA AAGGGATACA 
AGTACTTCTT CAGTTGAGCG GAGAAATGGG GTATTACAAC AGCGAGGAGC AGCAGGACCT 
CAATTCCATC AGAGAGATGA TGAAGAACTA ATTCCTTACA GTAAACCTAA CTTCCTATCA 
CGGAGTCAGA TGTCAAGTAA TTGTTCCACA ACAGGAAGCA GCTCCTCCAG AGGGTCAGCT 
GGATCTAGAG GTCAGGCATC TGGAAGGAGC AAAACAGGAG ACCGGAAAGA GGAAAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822435.1
CDS	362..4624
Translation	MLRYLLKTLLQMNLFADSIPGENSNSTDLFYQFNSSFPAFNDSLIDFINLTNFNGSRPRLEDFPPRIVEHPSDHIVSKGEPATLNCKAEGRPTPIIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYICVARNYLGESVSRNASLEVAILRDDFRQAPNDVVVAAGEPAVMECIPPRGHPEPTISWKKDNMRISDKDERITIRGGKLMISNTRKSDAGMYICVATNMVGERDSEPAQLVVFERPTFIRRPINQVVLADDMVDFNCEVQGDPVPTARWRKEEGELPRGRYEILNDNTLRISRVSAEDEGTYTCITENSVGKSEASGSLGVHVPPQLVTRPRDQIVVQGRTVTFQCETKGNPPPAVFWQKEGSQILLFPSQPPQTVGRFSVSPSGELTIIDVQTGDSGYYMCQAISVAGSILAKALLEVEDATSDRIPPIIRRGPANQTLAVDSTVQMHCQVSGNPLPSIHWLKDGQRIVGSDPRIGVLDSGNLQITHLQVTDSGVYTCVATSSTGETTWSGFVEVQEKGSPIHVQLSEPSLVPGSPSKPLVTDVTKNSVTLTWQPNLQAGNTQVNSYIIEAFSQSAGSTWQTVADNVKVEKHTVVGLNPNTIYLFIIRAVNSYGLSDPSPISEPVRTQDVSPTRQGVDHRQVQRQLGEVVVQLQQPIILTSSTVQVTWTVDRQAQFIQGYRVMYRPKSSVWLVQDVKSPSERNTILVDLKKGTEYEIKIRPYFDEFQGMDSDVLLIRTPEEVPSAPPQAVSVVTLGTNNSTNISISWEPPIVEHQNGIIQDYRIWVLGNETRYHINRTVDGTVHSTVIRGLSPGVLYHVEVAAATSAGFGVKSQPITIQIKPSSDQVPVASGENVSLTEQITDVVKQPAFIAGIGGACWVILMCFSVWIYCRRKKRKELSHYTASFAYTPAVSFPESSGHITARPGMLATSASNYPWLADSWPATNLLRNGKPKEPAGNCCPGNHNPSERYYNDAGISNYIAQTEKFGTGVSDGPIYSTIDPTTDDMRTFNGAFSQHTTPYATTPVVPYGGQNMHSPELEENQWNVQAGTSGVLYAQPDRTKAENGPKSGKQKAIGKPVKTPSLNWTEILPPPPPANELSQYQEEDTDIEMSSDTEEWCPPLPERTYLMENIQEGCPLPPPRGEASSPAHSYGQQSTATLTPSPQDEMHAAEDIPRLHQFEIPPHPRRLPSGSVPIPRAPSPPLTQSNPSLHTATEGAEAQRQGHRRNLSQNPNLSAENVNLPNSGKSQLGENISTPPGQKSRAKKKSKSGHYRREAKQGDLPPPPEPPPADEEPCSPHTNGLRMECSASSLERDTSTSSVERRNGVLQQRGAAGPQFHQRDDEELIPYSKPNFLSRSQMSSNCSTTGSSSSRGSAGSRGQASGRSKTGDRKEEKR

Target ORF information:

RefSeq Version	XM_012966981.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966981.1

ORF Insert Sequence:

ATGCTGCGCT ATCTGCTAAA GACTCTGCTG CAGATGAACC TCTTTGCGGA CTCCATCCCA 
GGCGAGAACT CCAATTCCAC TGATTTATTT TACCAGTTTA ACTCATCCTT TCCTGCTTTC 
AATGACAGCT TAATTGACTT CATCAACCTG ACCAACTTCA ATGGTTCTAG ACCTCGACTA 
GAAGATTTCC CGCCTCGAAT AGTGGAGCAT CCTTCTGATC ACATTGTCTC AAAGGGGGAA 
CCAGCTACTT TAAACTGCAA AGCAGAAGGC CGACCAACAC CTATTATTGA GTGGTACAAG 
GATGGGGAAA GGGTGGAGAC AGACAAAGAC GATCCTCGGT CACATCGCAT GCTGTTACCC 
AGTGGCTCAC TTTTCTTTCT ACGAATTGTT CATGGCCGTC GGAGTAAACC CGATGAAGGG 
GTATACATTT GTGTTGCAAG AAATTACCTT GGAGAGTCTG TCAGTCGAAA TGCATCTCTG 
GAAGTAGCAA TATTAAGAGA TGATTTTCGT CAGGCACCCA ATGATGTGGT AGTAGCAGCA 
GGAGAACCAG CAGTCATGGA ATGCATTCCA CCCAGAGGTC ATCCTGAACC CACTATTTCC 
TGGAAAAAGG ATAACATGCG CATAAGTGAT AAAGATGAAC GCATCACAAT TCGAGGTGGG 
AAACTAATGA TTTCCAATAC GAGAAAGAGC GATGCAGGCA TGTACATCTG TGTGGCTACA 
AACATGGTGG GAGAACGAGA TAGCGAACCT GCTCAGCTTG TGGTCTTTGA ACGCCCCACA 
TTTATTAGAA GGCCCATAAA CCAAGTTGTT CTAGCAGATG ATATGGTGGA TTTTAACTGT 
GAAGTTCAGG GTGATCCTGT ACCTACTGCT CGCTGGCGTA AGGAAGAAGG AGAACTACCA 
CGGGGCCGGT ATGAAATCCT TAATGATAAT ACATTGCGGA TCTCTCGGGT GAGTGCTGAG 
GATGAAGGAA CTTACACTTG TATCACTGAA AATAGTGTTG GAAAATCAGA GGCTTCAGGA 
TCTCTTGGCG TTCATGTTCC TCCACAACTG GTCACCCGAC CACGAGACCA AATAGTAGTT 
CAAGGACGTA CTGTTACATT CCAGTGTGAA ACCAAAGGGA ATCCACCACC AGCTGTATTT 
TGGCAGAAAG AAGGAAGTCA GATCCTTCTT TTCCCAAGCC AGCCTCCCCA AACAGTGGGA 
CGATTTTCTG TCTCTCCGAG TGGAGAACTG ACCATAATCG ATGTTCAGAC AGGAGACTCA 
GGGTACTACA TGTGTCAAGC CATTAGTGTG GCTGGAAGTA TCTTAGCTAA GGCTCTACTT 
GAAGTGGAAG ATGCAACATC AGATCGAATT CCTCCTATAA TTCGTAGAGG ACCAGCCAAT 
CAAACCCTTG CTGTTGACTC TACAGTGCAG ATGCATTGCC AGGTATCTGG TAATCCTCTT 
CCAAGTATAC ATTGGCTCAA AGATGGCCAA AGGATTGTGG GAAGTGACCC TCGAATAGGA 
GTGTTGGACA GTGGAAATTT ACAGATCACC CATTTACAGG TGACTGATTC AGGTGTATAT 
ACATGTGTGG CTACAAGTTC AACTGGGGAG ACTACATGGA GTGGATTTGT TGAAGTTCAA 
GAAAAAGGAT CTCCAATACA TGTGCAGCTT TCTGAGCCCA GCCTTGTTCC TGGATCTCCT 
TCCAAACCTC TTGTCACTGA CGTTACCAAA AATAGTGTTA CTTTAACATG GCAACCCAAT 
CTGCAAGCAG GAAACACACA AGTTAATTCC TATATCATTG AAGCATTCAG CCAATCAGCA 
GGAAGCACCT GGCAAACAGT TGCAGACAAC GTGAAAGTAG AAAAGCACAC GGTGGTTGGG 
TTGAACCCAA ATACAATTTA TCTTTTCATC ATACGAGCAG TAAATTCATA TGGACTTAGT 
GACCCCAGCC CAATTTCAGA ACCAGTGCGA ACACAAGATG TCAGTCCAAC GAGACAAGGT 
GTTGATCATA GGCAAGTGCA GCGCCAACTA GGGGAAGTGG TGGTTCAGCT TCAACAACCG 
ATTATTCTTA CATCCTCTAC TGTGCAAGTT ACATGGACAG TGGATCGGCA AGCTCAGTTC 
ATCCAAGGTT ACCGGGTGAT GTATCGTCCA AAATCTAGTG TTTGGCTTGT ACAGGATGTG 
AAGTCTCCCT CTGAACGGAA TACTATCCTT GTGGACCTCA AAAAAGGCAC AGAATATGAA 
ATCAAGATCC GTCCTTATTT TGATGAATTT CAAGGCATGG ACAGTGATGT GCTTTTGATC 
CGTACACCTG AAGAAGTGCC AAGTGCCCCG CCTCAAGCAG TATCAGTTGT TACATTAGGA 
ACGAATAACA GCACAAATAT TAGTATATCG TGGGAACCTC CTATAGTGGA ACATCAAAAT 
GGAATTATTC AAGATTATCG GATTTGGGTC TTGGGAAATG AAACACGCTA TCATATCAAC 
AGAACAGTAG ATGGTACTGT CCACTCCACT GTAATAAGAG GCCTCAGTCC TGGAGTTCTT 
TACCATGTGG AAGTGGCTGC TGCAACAAGT GCTGGTTTTG GTGTAAAAAG CCAACCAATC 
ACCATCCAGA TAAAGCCTTC TTCAGATCAA GTTCCAGTTG CCAGTGGAGA AAATGTCAGC 
TTGACTGAAC AAATCACAGA TGTTGTTAAA CAGCCTGCAT TCATAGCTGG CATTGGAGGG 
GCATGCTGGG TAATTCTAAT GTGCTTTAGT GTCTGGATCT ACTGCCGAAG GAAGAAGAGG 
AAAGAACTGA GCCATTATAC TGCTTCCTTT GCATACACAC CAGCAGTATC TTTTCCAGAA 
AGCTCAGGGC ATATTACTGC AAGACCAGGA ATGCTAGCTA CAAGTGCATC CAACTACCCT 
TGGCTAGCAG ATTCATGGCC AGCAACCAAC TTACTACGAA ACGGAAAGCC AAAAGAACCT 
GCAGGGAATT GTTGTCCAGG AAATCACAAT CCTTCAGAGA GATATTATAA TGATGCTGGC 
ATTTCAAACT ATATTGCTCA GACTGAGAAG TTCGGAACAG GTGTTTCAGA TGGTCCCATA 
TACAGTACAA TTGATCCAAC CACTGATGAC ATGCGAACTT TTAATGGAGC TTTCTCTCAG 
CATACTACAC CTTATGCAAC AACACCTGTT GTACCGTATG GAGGACAAAA CATGCATTCT 
CCTGAATTAG AAGAGAACCA GTGGAATGTA CAGGCTGGAA CATCTGGTGT ACTTTATGCA 
CAACCAGATC GAACTAAAGC AGAGAATGGT CCCAAATCTG GAAAGCAGAA GGCCATTGGA 
AAACCAGTAA AAACACCATC ACTAAATTGG ACAGAAATTC TTCCACCACC CCCGCCTGCC 
AATGAGCTGA GTCAATACCA AGAAGAAGAT ACAGATATAG AGATGAGCTC CGACACAGAA 
GAATGGTGTC CACCATTGCC TGAAAGAACT TACCTCATGG AAAACATTCA AGAAGGCTGC 
CCACTTCCTC CACCTCGGGG TGAGGCTTCT TCTCCTGCTC ATTCTTACGG TCAGCAGTCA 
ACAGCCACTC TAACACCATC TCCCCAGGAT GAGATGCATG CTGCAGAAGA TATTCCAAGG 
CTACATCAGT TTGAGATACC CCCTCATCCT AGACGACTGC CATCTGGATC GGTACCAATT 
CCAAGAGCTC CAAGTCCTCC TCTGACACAA TCAAATCCTA GTCTTCATAC AGCAACGGAA 
GGTGCAGAAG CACAACGGCA GGGACACAGG CGGAACTTAA GCCAAAATCC TAATCTAAGT 
GCTGAAAATG TCAACTTGCC AAACAGCGGA AAGTCACAAC TAGGAGAAAA TATAAGCACA 
CCTCCAGGAC AGAAATCGCG AGCAAAGAAG AAGTCAAAAT CAGGTCACTA TCGCAGGGAA 
GCAAAACAAG GAGATCTTCC TCCACCACCT GAGCCCCCTC CAGCAGATGA GGAACCATGT 
TCTCCACACA CTAATGGACT ACGTATGGAA TGTTCAGCCT CGTCCTTGGA AAGGGATACA 
AGTACTTCTT CAGTTGAGCG GAGAAATGGG GTATTACAAC AGCGAGGAGC AGCAGGACCT 
CAATTCCATC AGAGAGATGA TGAAGAACTA ATTCCTTACA GTAAACCTAA CTTCCTATCA 
CGGAGTCAGA TGTCAAGTAA TTGTTCCACA ACAGGAAGCA GCTCCTCCAG AGGGTCAGCT 
GGATCTAGAG GTCAGGCATC TGGAAGGAGC AAAACAGGAG ACCGGAAAGA GGAAAAGAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robo3 ( XM_012966982.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822436.1 Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X4, mRNA.

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CloneID	OXa35709
Clone ID Related Accession (Same CDS sequence)	XM_012966982.2
Accession Version	XM_012966982.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	roundabout homolog 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966982.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGGC AGAAGATGAG CTTTATGGGC AAAACCTTCA CCTGCGTTTT ATTTGTGTCC 
GTATGTTCCG TCCTAGGTTC TAGACCTCGA CTAGAAGATT TCCCGCCTCG AATAGTGGAG 
CATCCTTCTG ATCACATTGT CTCAAAGGGG GAACCAGCTA CTTTAAACTG CAAAGCAGAA 
GGCCGACCAA CACCTATTAT TGAGTGGTAC AAGGATGGGG AAAGGGTGGA GACAGACAAA 
GACGATCCTC GGTCACATCG CATGCTGTTA CCCAGTGGCT CACTTTTCTT TCTACGAATT 
GTTCATGGCC GTCGGAGTAA ACCCGATGAA GGGGTATACA TTTGTGTTGC AAGAAATTAC 
CTTGGAGAGT CTGTCAGTCG AAATGCATCT CTGGAAGTAG CAATATTAAG AGATGATTTT 
CGTCAGGCAC CCAATGATGT GGTAGTAGCA GCAGGAGAAC CAGCAGTCAT GGAATGCATT 
CCACCCAGAG GTCATCCTGA ACCCACTATT TCCTGGAAAA AGGATAACAT GCGCATAAGT 
GATAAAGATG AACGCATCAC AATTCGAGGT GGGAAACTAA TGATTTCCAA TACGAGAAAG 
AGCGATGCAG GCATGTACAT CTGTGTGGCT ACAAACATGG TGGGAGAACG AGATAGCGAA 
CCTGCTCAGC TTGTGGTCTT TGAACGCCCC ACATTTATTA GAAGGCCCAT AAACCAAGTT 
GTTCTAGCAG ATGATATGGT GGATTTTAAC TGTGAAGTTC AGGGTGATCC TGTACCTACT 
GCTCGCTGGC GTAAGGAAGA AGGAGAACTA CCACGGGGCC GGTATGAAAT CCTTAATGAT 
AATACATTGC GGATCTCTCG GGTGAGTGCT GAGGATGAAG GAACTTACAC TTGTATCACT 
GAAAATAGTG TTGGAAAATC AGAGGCTTCA GGATCTCTTG GCGTTCATGT TGGCCCATTT 
TTTCCTCCAC AACTGGTCAC CCGACCACGA GACCAAATAG TAGTTCAAGG ACGTACTGTT 
ACATTCCAGT GTGAAACCAA AGGGAATCCA CCACCAGCTG TATTTTGGCA GAAAGAAGGA 
AGTCAGATCC TTCTTTTCCC AAGCCAGCCT CCCCAAACAG TGGGACGATT TTCTGTCTCT 
CCGAGTGGAG AACTGACCAT AATCGATGTT CAGACAGGAG ACTCAGGGTA CTACATGTGT 
CAAGCCATTA GTGTGGCTGG AAGTATCTTA GCTAAGGCTC TACTTGAAGT GGAAGATGCA 
ACATCAGATC GAATTCCTCC TATAATTCGT AGAGGACCAG CCAATCAAAC CCTTGCTGTT 
GACTCTACAG TGCAGATGCA TTGCCAGGTA TCTGGTAATC CTCTTCCAAG TATACATTGG 
CTCAAAGATG GCCAAAGGAT TGTGGGAAGT GACCCTCGAA TAGGAGTGTT GGACAGTGGA 
AATTTACAGA TCACCCATTT ACAGGTGACT GATTCAGGTG TATATACATG TGTGGCTACA 
AGTTCAACTG GGGAGACTAC ATGGAGTGGA TTTGTTGAAG TTCAAGAAAA AGGATCTCCA 
ATACATGTGC AGCTTTCTGA GCCCAGCCTT GTTCCTGGAT CTCCTTCCAA ACCTCTTGTC 
ACTGACGTTA CCAAAAATAG TGTTACTTTA ACATGGCAAC CCAATCTGCA AGCAGGAAAC 
ACACAAGTTA ATTCCTATAT CATTGAAGCA TTCAGCCAAT CAGCAGGAAG CACCTGGCAA 
ACAGTTGCAG ACAACGTGAA AGTAGAAAAG CACACGGTGG TTGGGTTGAA CCCAAATACA 
ATTTATCTTT TCATCATACG AGCAGTAAAT TCATATGGAC TTAGTGACCC CAGCCCAATT 
TCAGAACCAG TGCGAACACA AGATGTCAGT CCAACGAGAC AAGGTGTTGA TCATAGGCAA 
GTGCAGCGCC AACTAGGGGA AGTGGTGGTT CAGCTTCAAC AACCGATTAT TCTTACATCC 
TCTACTGTGC AAGTTACATG GACAGTGGAT CGGCAAGCTC AGTTCATCCA AGGTTACCGG 
GTGATGTATC GTCCAAAATC TAGTGTTTGG CTTGTACAGG ATGTGAAGTC TCCCTCTGAA 
CGGAATACTA TCCTTGTGGA CCTCAAAAAA GGCACAGAAT ATGAAATCAA GATCCGTCCT 
TATTTTGATG AATTTCAAGG CATGGACAGT GATGTGCTTT TGATCCGTAC ACCTGAAGAA 
GTGCCAAGTG CCCCGCCTCA AGCAGTATCA GTTGTTACAT TAGGAACGAA TAACAGCACA 
AATATTAGTA TATCGTGGGA ACCTCCTATA GTGGAACATC AAAATGGAAT TATTCAAGAT 
TATCGGATTT GGGTCTTGGG AAATGAAACA CGCTATCATA TCAACAGAAC AGTAGATGGT 
ACTGTCCACT CCACTGTAAT AAGAGGCCTC AGTCCTGGAG TTCTTTACCA TGTGGAAGTG 
GCTGCTGCAA CAAGTGCTGG TTTTGGTGTA AAAAGCCAAC CAATCACCAT CCAGATAAAG 
CCTTCTTCAG ATCAAGTTCC AGTTGCCAGT GGAGAAAATG TCAGCTTGAC TGAACAAATC 
ACAGATGTTG TTAAACAGCC TGCATTCATA GCTGGCATTG GAGGGGCATG CTGGGTAATT 
CTAATGTGCT TTAGTGTCTG GATCTACTGC CGAAGGAAGA AGAGGAAAGA ACTGAGCCAT 
TATACTGCTT CCTTTGCATA CACACCAGCA GTATCTTTTC CAGAAAGCTC AGGGCATATT 
ACTGCAAGAC CAGGAATGCT AGCTACAAGT GCATCCAACT ACCCTTGGCT AGCAGATTCA 
TGGCCAGCAA CCAACTTACT ACGAAACGGA AAGCCAAAAG AACCTGCAGG GAATTGTTGT 
CCAGGAAATC ACAATCCTTC AGAGAGATAT TATAATGATG CTGGCATTTC AAACTATATT 
GCTCAGACTG AGAAGTTCGG AACAGGTGTT TCAGATGGTC CCATATACAG TACAATTGAT 
CCAACCACTG ATGACATGCG AACTTTTAAT GGAGCTTTCT CTCAGCATAC TACACCTTAT 
GCAACAACAC CTGTTGTACC GTATGGAGGA CAAAACATGC ATTCTCCTGA ATTAGAAGAG 
AACCAGTGGA ATGTACAGGC TGGAACATCT GGTGTACTTT ATGCACAACC AGATCGAACT 
AAAGCAGAGA ATGGTCCCAA ATCTGGAAAG CAGAAGGCCA TTGGAAAACC AGTAAAAACA 
CCATCACTAA ATTGGACAGA AATTCTTCCA CCACCCCCGC CTGCCAATGA GCTGAGTCAA 
TACCAAGAAG AAGATACAGA TATAGAGATG AGCTCCGACA CAGAAGAATG GTGTCCACCA 
TTGCCTGAAA GAACTTACCT CATGGAAAAC ATTCAAGAAG GCTGCCCACT TCCTCCACCT 
CGGGGTGAGG CTTCTTCTCC TGCTCATTCT TACGGTCAGC AGTCAACAGC CACTCTAACA 
CCATCTCCCC AGGATGAGAT GCATGCTGCA GAAGATATTC CAAGGCTACA TCAGTTTGAG 
ATACCCCCTC ATCCTAGACG ACTGCCATCT GGATCGGTAC CAATTCCAAG AGCTCCAAGT 
CCTCCTCTGA CACAATCAAA TCCTAGTCTT CATACAGCAA CGGAAGGTGC AGAAGCACAA 
CGGCAGGGAC ACAGGCGGAA CTTAAGCCAA AATCCTAATC TAAGTGCTGA AAATGTCAAC 
TTGCCAAACA GCGGAAAGTC ACAACTAGGA GAAAATATAA GCACACCTCC AGGACAGAAA 
TCGCGAGCAA AGAAGAAGTC AAAATCAGGT CACTATCGCA GGGAAGCAAA ACAAGGAGAT 
CTTCCTCCAC CACCTGAGCC CCCTCCAGCA GATGAGGAAC CATGTTCTCC ACACACTAAT 
GGACTACGTA TGGAATGTTC AGCCTCGTCC TTGGAAAGGG ATACAAGTAC TTCTTCAGTT 
GAGCGGAGAA ATGGGGTATT ACAACAGCGA GGAGCAGCAG GACCTCAATT CCATCAGAGA 
GATGATGAAG AACTAATTCC TTACAGTAAA CCTAACTTCC TATCACGGAG TCAGATGTCA 
AGTAATTGTT CCACAACAGG AAGCAGCTCC TCCAGAGGGT CAGCTGGATC TAGAGGTCAG 
GCATCTGGAA GGAGCAAAAC AGGAGACCGG AAAGAGGAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822436.1
CDS	516..4703
Translation	MERQKMSFMGKTFTCVLFVSVCSVLGSRPRLEDFPPRIVEHPSDHIVSKGEPATLNCKAEGRPTPIIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGVYICVARNYLGESVSRNASLEVAILRDDFRQAPNDVVVAAGEPAVMECIPPRGHPEPTISWKKDNMRISDKDERITIRGGKLMISNTRKSDAGMYICVATNMVGERDSEPAQLVVFERPTFIRRPINQVVLADDMVDFNCEVQGDPVPTARWRKEEGELPRGRYEILNDNTLRISRVSAEDEGTYTCITENSVGKSEASGSLGVHVGPFFPPQLVTRPRDQIVVQGRTVTFQCETKGNPPPAVFWQKEGSQILLFPSQPPQTVGRFSVSPSGELTIIDVQTGDSGYYMCQAISVAGSILAKALLEVEDATSDRIPPIIRRGPANQTLAVDSTVQMHCQVSGNPLPSIHWLKDGQRIVGSDPRIGVLDSGNLQITHLQVTDSGVYTCVATSSTGETTWSGFVEVQEKGSPIHVQLSEPSLVPGSPSKPLVTDVTKNSVTLTWQPNLQAGNTQVNSYIIEAFSQSAGSTWQTVADNVKVEKHTVVGLNPNTIYLFIIRAVNSYGLSDPSPISEPVRTQDVSPTRQGVDHRQVQRQLGEVVVQLQQPIILTSSTVQVTWTVDRQAQFIQGYRVMYRPKSSVWLVQDVKSPSERNTILVDLKKGTEYEIKIRPYFDEFQGMDSDVLLIRTPEEVPSAPPQAVSVVTLGTNNSTNISISWEPPIVEHQNGIIQDYRIWVLGNETRYHINRTVDGTVHSTVIRGLSPGVLYHVEVAAATSAGFGVKSQPITIQIKPSSDQVPVASGENVSLTEQITDVVKQPAFIAGIGGACWVILMCFSVWIYCRRKKRKELSHYTASFAYTPAVSFPESSGHITARPGMLATSASNYPWLADSWPATNLLRNGKPKEPAGNCCPGNHNPSERYYNDAGISNYIAQTEKFGTGVSDGPIYSTIDPTTDDMRTFNGAFSQHTTPYATTPVVPYGGQNMHSPELEENQWNVQAGTSGVLYAQPDRTKAENGPKSGKQKAIGKPVKTPSLNWTEILPPPPPANELSQYQEEDTDIEMSSDTEEWCPPLPERTYLMENIQEGCPLPPPRGEASSPAHSYGQQSTATLTPSPQDEMHAAEDIPRLHQFEIPPHPRRLPSGSVPIPRAPSPPLTQSNPSLHTATEGAEAQRQGHRRNLSQNPNLSAENVNLPNSGKSQLGENISTPPGQKSRAKKKSKSGHYRREAKQGDLPPPPEPPPADEEPCSPHTNGLRMECSASSLERDTSTSSVERRNGVLQQRGAAGPQFHQRDDEELIPYSKPNFLSRSQMSSNCSTTGSSSSRGSAGSRGQASGRSKTGDRKEEKR

Target ORF information:

RefSeq Version	XM_012966982.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis roundabout guidance receptor 3 (robo3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966982.2

ORF Insert Sequence:

ATGGAAAGGC AGAAGATGAG CTTTATGGGC AAAACCTTCA CCTGCGTTTT ATTTGTGTCC 
GTATGTTCCG TCCTAGGTTC TAGACCTCGA CTAGAAGATT TCCCGCCTCG AATAGTGGAG 
CATCCTTCTG ATCACATTGT CTCAAAGGGG GAACCAGCTA CTTTAAACTG CAAAGCAGAA 
GGCCGACCAA CACCTATTAT TGAGTGGTAC AAGGATGGGG AAAGGGTGGA GACAGACAAA 
GACGATCCTC GGTCACATCG CATGCTGTTA CCCAGTGGCT CACTTTTCTT TCTACGAATT 
GTTCATGGCC GTCGGAGTAA ACCCGATGAA GGGGTATACA TTTGTGTTGC AAGAAATTAC 
CTTGGAGAGT CTGTCAGTCG AAATGCATCT CTGGAAGTAG CAATATTAAG AGATGATTTT 
CGTCAGGCAC CCAATGATGT GGTAGTAGCA GCAGGAGAAC CAGCAGTCAT GGAATGCATT 
CCACCCAGAG GTCATCCTGA ACCCACTATT TCCTGGAAAA AGGATAACAT GCGCATAAGT 
GATAAAGATG AACGCATCAC AATTCGAGGT GGGAAACTAA TGATTTCCAA TACGAGAAAG 
AGCGATGCAG GCATGTACAT CTGTGTGGCT ACAAACATGG TGGGAGAACG AGATAGCGAA 
CCTGCTCAGC TTGTGGTCTT TGAACGCCCC ACATTTATTA GAAGGCCCAT AAACCAAGTT 
GTTCTAGCAG ATGATATGGT GGATTTTAAC TGTGAAGTTC AGGGTGATCC TGTACCTACT 
GCTCGCTGGC GTAAGGAAGA AGGAGAACTA CCACGGGGCC GGTATGAAAT CCTTAATGAT 
AATACATTGC GGATCTCTCG GGTGAGTGCT GAGGATGAAG GAACTTACAC TTGTATCACT 
GAAAATAGTG TTGGAAAATC AGAGGCTTCA GGATCTCTTG GCGTTCATGT TGGCCCATTT 
TTTCCTCCAC AACTGGTCAC CCGACCACGA GACCAAATAG TAGTTCAAGG ACGTACTGTT 
ACATTCCAGT GTGAAACCAA AGGGAATCCA CCACCAGCTG TATTTTGGCA GAAAGAAGGA 
AGTCAGATCC TTCTTTTCCC AAGCCAGCCT CCCCAAACAG TGGGACGATT TTCTGTCTCT 
CCGAGTGGAG AACTGACCAT AATCGATGTT CAGACAGGAG ACTCAGGGTA CTACATGTGT 
CAAGCCATTA GTGTGGCTGG AAGTATCTTA GCTAAGGCTC TACTTGAAGT GGAAGATGCA 
ACATCAGATC GAATTCCTCC TATAATTCGT AGAGGACCAG CCAATCAAAC CCTTGCTGTT 
GACTCTACAG TGCAGATGCA TTGCCAGGTA TCTGGTAATC CTCTTCCAAG TATACATTGG 
CTCAAAGATG GCCAAAGGAT TGTGGGAAGT GACCCTCGAA TAGGAGTGTT GGACAGTGGA 
AATTTACAGA TCACCCATTT ACAGGTGACT GATTCAGGTG TATATACATG TGTGGCTACA 
AGTTCAACTG GGGAGACTAC ATGGAGTGGA TTTGTTGAAG TTCAAGAAAA AGGATCTCCA 
ATACATGTGC AGCTTTCTGA GCCCAGCCTT GTTCCTGGAT CTCCTTCCAA ACCTCTTGTC 
ACTGACGTTA CCAAAAATAG TGTTACTTTA ACATGGCAAC CCAATCTGCA AGCAGGAAAC 
ACACAAGTTA ATTCCTATAT CATTGAAGCA TTCAGCCAAT CAGCAGGAAG CACCTGGCAA 
ACAGTTGCAG ACAACGTGAA AGTAGAAAAG CACACGGTGG TTGGGTTGAA CCCAAATACA 
ATTTATCTTT TCATCATACG AGCAGTAAAT TCATATGGAC TTAGTGACCC CAGCCCAATT 
TCAGAACCAG TGCGAACACA AGATGTCAGT CCAACGAGAC AAGGTGTTGA TCATAGGCAA 
GTGCAGCGCC AACTAGGGGA AGTGGTGGTT CAGCTTCAAC AACCGATTAT TCTTACATCC 
TCTACTGTGC AAGTTACATG GACAGTGGAT CGGCAAGCTC AGTTCATCCA AGGTTACCGG 
GTGATGTATC GTCCAAAATC TAGTGTTTGG CTTGTACAGG ATGTGAAGTC TCCCTCTGAA 
CGGAATACTA TCCTTGTGGA CCTCAAAAAA GGCACAGAAT ATGAAATCAA GATCCGTCCT 
TATTTTGATG AATTTCAAGG CATGGACAGT GATGTGCTTT TGATCCGTAC ACCTGAAGAA 
GTGCCAAGTG CCCCGCCTCA AGCAGTATCA GTTGTTACAT TAGGAACGAA TAACAGCACA 
AATATTAGTA TATCGTGGGA ACCTCCTATA GTGGAACATC AAAATGGAAT TATTCAAGAT 
TATCGGATTT GGGTCTTGGG AAATGAAACA CGCTATCATA TCAACAGAAC AGTAGATGGT 
ACTGTCCACT CCACTGTAAT AAGAGGCCTC AGTCCTGGAG TTCTTTACCA TGTGGAAGTG 
GCTGCTGCAA CAAGTGCTGG TTTTGGTGTA AAAAGCCAAC CAATCACCAT CCAGATAAAG 
CCTTCTTCAG ATCAAGTTCC AGTTGCCAGT GGAGAAAATG TCAGCTTGAC TGAACAAATC 
ACAGATGTTG TTAAACAGCC TGCATTCATA GCTGGCATTG GAGGGGCATG CTGGGTAATT 
CTAATGTGCT TTAGTGTCTG GATCTACTGC CGAAGGAAGA AGAGGAAAGA ACTGAGCCAT 
TATACTGCTT CCTTTGCATA CACACCAGCA GTATCTTTTC CAGAAAGCTC AGGGCATATT 
ACTGCAAGAC CAGGAATGCT AGCTACAAGT GCATCCAACT ACCCTTGGCT AGCAGATTCA 
TGGCCAGCAA CCAACTTACT ACGAAACGGA AAGCCAAAAG AACCTGCAGG GAATTGTTGT 
CCAGGAAATC ACAATCCTTC AGAGAGATAT TATAATGATG CTGGCATTTC AAACTATATT 
GCTCAGACTG AGAAGTTCGG AACAGGTGTT TCAGATGGTC CCATATACAG TACAATTGAT 
CCAACCACTG ATGACATGCG AACTTTTAAT GGAGCTTTCT CTCAGCATAC TACACCTTAT 
GCAACAACAC CTGTTGTACC GTATGGAGGA CAAAACATGC ATTCTCCTGA ATTAGAAGAG 
AACCAGTGGA ATGTACAGGC TGGAACATCT GGTGTACTTT ATGCACAACC AGATCGAACT 
AAAGCAGAGA ATGGTCCCAA ATCTGGAAAG CAGAAGGCCA TTGGAAAACC AGTAAAAACA 
CCATCACTAA ATTGGACAGA AATTCTTCCA CCACCCCCGC CTGCCAATGA GCTGAGTCAA 
TACCAAGAAG AAGATACAGA TATAGAGATG AGCTCCGACA CAGAAGAATG GTGTCCACCA 
TTGCCTGAAA GAACTTACCT CATGGAAAAC ATTCAAGAAG GCTGCCCACT TCCTCCACCT 
CGGGGTGAGG CTTCTTCTCC TGCTCATTCT TACGGTCAGC AGTCAACAGC CACTCTAACA 
CCATCTCCCC AGGATGAGAT GCATGCTGCA GAAGATATTC CAAGGCTACA TCAGTTTGAG 
ATACCCCCTC ATCCTAGACG ACTGCCATCT GGATCGGTAC CAATTCCAAG AGCTCCAAGT 
CCTCCTCTGA CACAATCAAA TCCTAGTCTT CATACAGCAA CGGAAGGTGC AGAAGCACAA 
CGGCAGGGAC ACAGGCGGAA CTTAAGCCAA AATCCTAATC TAAGTGCTGA AAATGTCAAC 
TTGCCAAACA GCGGAAAGTC ACAACTAGGA GAAAATATAA GCACACCTCC AGGACAGAAA 
TCGCGAGCAA AGAAGAAGTC AAAATCAGGT CACTATCGCA GGGAAGCAAA ACAAGGAGAT 
CTTCCTCCAC CACCTGAGCC CCCTCCAGCA GATGAGGAAC CATGTTCTCC ACACACTAAT 
GGACTACGTA TGGAATGTTC AGCCTCGTCC TTGGAAAGGG ATACAAGTAC TTCTTCAGTT 
GAGCGGAGAA ATGGGGTATT ACAACAGCGA GGAGCAGCAG GACCTCAATT CCATCAGAGA 
GATGATGAAG AACTAATTCC TTACAGTAAA CCTAACTTCC TATCACGGAG TCAGATGTCA 
AGTAATTGTT CCACAACAGG AAGCAGCTCC TCCAGAGGGT CAGCTGGATC TAGAGGTCAG 
GCATCTGGAA GGAGCAAAAC AGGAGACCGG AAAGAGGAAA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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robo3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

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