slc12a4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc12a4
Entrez Gene ID	100493471
Full Name	solute carrier family 12 (potassium/chloride transporter), member 4
Synonyms	kcc1
General protein information	Preferred Names solute carrier family 12 (potassium/chloride transporter), member 4 Names solute carrier family 12 member 4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002942670.4	XP_002942716.1	solute carrier family 12 member 4
XM_002942670.5	XP_002942716.1	solute carrier family 12 member 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC12A4	NP_005063.1
Gallus gallus (chicken)	SLC12A4	XP_003641964.1
Danio rerio (zebrafish)	slc12a4	XP_691291.2
Drosophila melanogaster (fruit fly)	kcc	NP_726377.1
Canis lupus familiaris (dog)	SLC12A4	NP_001010952.1
Rattus norvegicus (Norway rat)	Slc12a4	NP_062102.1
Caenorhabditis elegans (roundworm)	kcc-2	NP_001255280.1
Pan troglodytes (chimpanzee)	SLC12A4	XP_003315222.2
	SLC12A4	XP_001096326.2
Bos taurus (cattle)	SLC12A4	NP_001167562.1
Mus musculus (house mouse)	Slc12a4	NP_001240733.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011498	XP_317803.3
Xenopus tropicalis (tropical clawed frog)	LOC100493471	XP_002942716.1

Gene Ontology

The following slc12a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa56770	XM_002942670.5 Latest version!	Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa18989	XM_002942670.4 Latest version!	Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc12a4 ( XM_002942670.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002942716.1 Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.

Price & Availability↑

CloneID	OXa56770
Clone ID Related Accession (Same CDS sequence)	XM_002942670.5
Accession Version	XM_002942670.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002942670.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCACT TCACAGTTGT TCCAGTTGAA GAGCAAGCTA GATTGGGATG TGATTACTTG 
GAAGGCCTTA ACGGAGTGGA TTATAGGGAC ACAGGGCACA GTTCTCGGGA TCCCTACGAC 
AGCGTTAGTT CCGACGGTCA TGGAAATTAC AAAGAGAATA GTCCATTTCT TACTGGTTCA 
GAGGCTAATA AAGGAAATCA GTTTTATGAC AAAAATCTGG CCTTATTTGA GGAAGAGCTT 
GATATTCGAC CTAAAGTTTC ATCATTTCTC AGTCGTCTTG TTAACTACAC AAACCTTACC 
CAAGGTGCCA AAGAACATGA AGAGGCTGAG GAAGCTCGAA GAAAAGTTAA CAAATCTCCT 
TGCATGGGAA CAGTAATGGG TGTCTACCTT CCTTGCATGC AAAACATCCT TGGTGTAATC 
TTGTTTCTTC GCCTAACCTG GATTGTCGGC ACTGGTGGAG TACTACAGAC CCTTTTGATT 
ACTCTAATCT GCTGCTGCTG TACTCTGCTT ACAGCCATTT CAATGAGTGC AATAGCTACC 
AATGGAGTTG TGCCAGCTGG TGGATCATAC TTTATGATTT CTCGTTCCCT TGGCCCAGAA 
TTTGGAGGTG CTGTGGGTCT GTGCTTCTAT TTGGGGACTA CATTTGCAGG AGCTATGTAT 
ATTTTGGGAG CTATTGAGAT ACTTCTGATG TATATTGTTC CTGAGGCTGC CATCTTTCAC 
TCTCCAAACA CTCACGAAGA CTCTAGTGCA AAGCTAAACA ATATGCGTGT TTATGGCACA 
GTCTTTCTCA CTTTGATGGC AATCATTGTG TTTGTTGGCG TTAAGTATGT GAACAAATTT 
GCATCTCTCT TCTTGGCTTG TGTGATTATT TCCATCCTGT CAATATATGC TGGATCCATC 
AAATCTATCT TTGATCCTCC TGTTTTTCCG ATTTGCATGC TGGGCAATAG GACTCTTTCC 
AGAGATCGGT TTGAAATTTG TGCCAAAACT ATCATTCTAG ACAATGTAAC AAAGCCTACA 
ACATTATGGG AGCAGTTCTG TGGGTCCTCC AATTTTTCCA CCAGTAGCTG TGATCAATAT 
TTCCTTAAAA ACAATGTAAC TGAGATTGCT GGGATTCCAG GGGCTGCAAG TGGTATTTTA 
AAAGATAATC TGTGGAGTAA TTATTTACAG AAGGGGGAAA TTCTGGAAAA ATCTCAACTT 
TCTTCAGTTG ATGAACTAGG ATCCAAAAGT AATCTCCACC TGTATGTTCT GACAGACATC 
TCTGCTACAT TCACTGTACT TGTGGGCATT TTTTTTCCTT CTGTCACTGG TATCATGGCT 
GGAAGTAATC GGTCAGGGGA TCTGCGGGAT GCCCAGAAAT CCATTCCTGT AGGAACCATA 
CTTGCTATTA TCACCACCTC ATTTATTTAT TTTAGCACTG TTGTTCTGTT TGGCGCTTGC 
ATTGAAGGAG TGGTTTTGCG TGACAAATAT GGAGATGCAG TGAACAAAAA TTTGGTTGTG 
GGGACACTGT CTTGGCCTTC TCCATGGGTT ATTGTCATCG GTTCATTTTT TTCAACTTGT 
GGGGCTGGAT TGCAGAGCTT AACAGGAGCT CCACGGTTAC TACAAGCAAT TGCAAATGAC 
AATATTATTC CATTCCTGAG GGTATTTGGA CAGGGAAAAG TAAATGGTGA GCCAACCTGG 
GCCCTTCTAC TGACTGCTTT CATAGCAGAG TTAGGAATTC TGATTGCTTC ACTGGACATG 
GTGGCACCAA TCTTATCAAT GTTCTTTCTG ATGTGTTACT TATTTGTTAA TTTGGCATGT 
GCAGTGCAGA CACTCCTGAG AACGCCAAAC TGGAGACCAA GGTTTAAATA TTATCACTGG 
GCACTCTCTT TCTTTGGAAT GAGTATCTGC TTGGCCTTGA TGTTTGTCTG TTCCTGGTAC 
TATGCTTTGA TAGCAATGCT AATCGCTGGA ATGATTTACA AATATATAGA ATACCAAGGA 
GCAGAGAAGG AATGGGGAGA TGGCATCAGA GGTCTGTCTC TAAGTGCAGC CAGATATGCT 
TTACTAAGAC TGGAAGAGGG ACCACCACAC ACCAAAAACT GGAGACCTCA ACTGTTGGTT 
CTTTTAAAGC TAGATGAAGA CCTTCATGTT AAATATCCAC GTCTACTTAC ATTTTCCTCC 
CAGCTAAAAG CTGGCAAAGG TTTAACAATA ATTGGCACCA CCCTTCAAGG AAACTTTTTG 
GAGAGCTATG GAGAGGTTCA GGCTGCTGAG CAGACCATTA AAAACATGAT GGAGATTGAG 
AAAGTCAAAG GATTTTCCCA GGTTGTGGTT TCCAGCGAAG TTTGTGATGG AATTTCACAT 
CTAATTCAGT CATGTGGACT TGGAGGAATG AAGCATAATA CAGTACTGCT GGGATGGCCA 
TATGGTTGGA GGCAGAGTGA AGACCCTCGC TCTTGGAAGA CGTTTATTGA TGCTGTACGC 
TGCACAACTG CAGCTCGCTT GGCACTGATG GTGCCAAAAA ATGTTTCCTT TTACCCCAGC 
AACCATGAAC GCTATCTGGA GGGAAATATT GATGTCTGGT GGATTGTACA TGATGGCGGT 
ATGCTAATGC TCCTGCCTTT TCTACTGAAA CAACACAAGG TCTGGAAAAA GTGCAAAATG 
CGCATTTTTA CTGTGGCTCA GTTGGATGAC AATAGTATCC AAATGAAGAA AGACCTGGCA 
ACATTCCTTT ATCACTTGCG TCTAGAAGCA GAAGTTGAAG TAGTGGAAAT GCACAATAGT 
GACATCTCTG CATACACATA TGAGCGGACA CTGATGATGG AGCAGCGATC ACAGATGCTT 
AGACAGATGC GTCTCACAAA AACAGAAAGG GATAGGGAGG CTCAATTGGT GAAGGATAGA 
AATTCCATCA TTCCTGTTGA GAGTTACTAT TCTGATGAGG AGGATGAAGA GCCAGAATTG 
GAGCTAGCTA AGATACATAT GACTTGGACA AAAGATAAAT ATAATGCTCA ATGGAAAAAC 
AGAAACAATG AAACAATGCA TTTTAGGGAG CTTGCAGATA TAAAGCCGGA CCAGTCTAAT 
GTTCGACGGA TGCACACTGC AGTTAAACTA AATGAGGTCA TAGTGACAAG GTCACATGAT 
GCCAAATTAG TTCTTCTCAA CATGCCAGGT CCCCCCAAAA ATACAGATGG GGATGAAAAC 
TACATGGAAT TTCTTGAAGT ACTCACTGAA GGTTTGGAGA GGGTCCTGTT GGTACGTGGG 
GGAGGACGAG AAGTAATCAC CATCTATTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002942716.1
CDS	132..3404
Translation	MPHFTVVPVEEQARLGCDYLEGLNGVDYRDTGHSSRDPYDSVSSDGHGNYKENSPFLTGSEANKGNQFYDKNLALFEEELDIRPKVSSFLSRLVNYTNLTQGAKEHEEAEEARRKVNKSPCMGTVMGVYLPCMQNILGVILFLRLTWIVGTGGVLQTLLITLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGAIEILLMYIVPEAAIFHSPNTHEDSSAKLNNMRVYGTVFLTLMAIIVFVGVKYVNKFASLFLACVIISILSIYAGSIKSIFDPPVFPICMLGNRTLSRDRFEICAKTIILDNVTKPTTLWEQFCGSSNFSTSSCDQYFLKNNVTEIAGIPGAASGILKDNLWSNYLQKGEILEKSQLSSVDELGSKSNLHLYVLTDISATFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIITTSFIYFSTVVLFGACIEGVVLRDKYGDAVNKNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIANDNIIPFLRVFGQGKVNGEPTWALLLTAFIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFFGMSICLALMFVCSWYYALIAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFSSQLKAGKGLTIIGTTLQGNFLESYGEVQAAEQTIKNMMEIEKVKGFSQVVVSSEVCDGISHLIQSCGLGGMKHNTVLLGWPYGWRQSEDPRSWKTFIDAVRCTTAARLALMVPKNVSFYPSNHERYLEGNIDVWWIVHDGGMLMLLPFLLKQHKVWKKCKMRIFTVAQLDDNSIQMKKDLATFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTERDREAQLVKDRNSIIPVESYYSDEEDEEPELELAKIHMTWTKDKYNAQWKNRNNETMHFRELADIKPDQSNVRRMHTAVKLNEVIVTRSHDAKLVLLNMPGPPKNTDGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_002942670.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002942670.5

ORF Insert Sequence:

ATGCCTCACT TCACAGTTGT TCCAGTTGAA GAGCAAGCTA GATTGGGATG TGATTACTTG 
GAAGGCCTTA ACGGAGTGGA TTATAGGGAC ACAGGGCACA GTTCTCGGGA TCCCTACGAC 
AGCGTTAGTT CCGACGGTCA TGGAAATTAC AAAGAGAATA GTCCATTTCT TACTGGTTCA 
GAGGCTAATA AAGGAAATCA GTTTTATGAC AAAAATCTGG CCTTATTTGA GGAAGAGCTT 
GATATTCGAC CTAAAGTTTC ATCATTTCTC AGTCGTCTTG TTAACTACAC AAACCTTACC 
CAAGGTGCCA AAGAACATGA AGAGGCTGAG GAAGCTCGAA GAAAAGTTAA CAAATCTCCT 
TGCATGGGAA CAGTAATGGG TGTCTACCTT CCTTGCATGC AAAACATCCT TGGTGTAATC 
TTGTTTCTTC GCCTAACCTG GATTGTCGGC ACTGGTGGAG TACTACAGAC CCTTTTGATT 
ACTCTAATCT GCTGCTGCTG TACTCTGCTT ACAGCCATTT CAATGAGTGC AATAGCTACC 
AATGGAGTTG TGCCAGCTGG TGGATCATAC TTTATGATTT CTCGTTCCCT TGGCCCAGAA 
TTTGGAGGTG CTGTGGGTCT GTGCTTCTAT TTGGGGACTA CATTTGCAGG AGCTATGTAT 
ATTTTGGGAG CTATTGAGAT ACTTCTGATG TATATTGTTC CTGAGGCTGC CATCTTTCAC 
TCTCCAAACA CTCACGAAGA CTCTAGTGCA AAGCTAAACA ATATGCGTGT TTATGGCACA 
GTCTTTCTCA CTTTGATGGC AATCATTGTG TTTGTTGGCG TTAAGTATGT GAACAAATTT 
GCATCTCTCT TCTTGGCTTG TGTGATTATT TCCATCCTGT CAATATATGC TGGATCCATC 
AAATCTATCT TTGATCCTCC TGTTTTTCCG ATTTGCATGC TGGGCAATAG GACTCTTTCC 
AGAGATCGGT TTGAAATTTG TGCCAAAACT ATCATTCTAG ACAATGTAAC AAAGCCTACA 
ACATTATGGG AGCAGTTCTG TGGGTCCTCC AATTTTTCCA CCAGTAGCTG TGATCAATAT 
TTCCTTAAAA ACAATGTAAC TGAGATTGCT GGGATTCCAG GGGCTGCAAG TGGTATTTTA 
AAAGATAATC TGTGGAGTAA TTATTTACAG AAGGGGGAAA TTCTGGAAAA ATCTCAACTT 
TCTTCAGTTG ATGAACTAGG ATCCAAAAGT AATCTCCACC TGTATGTTCT GACAGACATC 
TCTGCTACAT TCACTGTACT TGTGGGCATT TTTTTTCCTT CTGTCACTGG TATCATGGCT 
GGAAGTAATC GGTCAGGGGA TCTGCGGGAT GCCCAGAAAT CCATTCCTGT AGGAACCATA 
CTTGCTATTA TCACCACCTC ATTTATTTAT TTTAGCACTG TTGTTCTGTT TGGCGCTTGC 
ATTGAAGGAG TGGTTTTGCG TGACAAATAT GGAGATGCAG TGAACAAAAA TTTGGTTGTG 
GGGACACTGT CTTGGCCTTC TCCATGGGTT ATTGTCATCG GTTCATTTTT TTCAACTTGT 
GGGGCTGGAT TGCAGAGCTT AACAGGAGCT CCACGGTTAC TACAAGCAAT TGCAAATGAC 
AATATTATTC CATTCCTGAG GGTATTTGGA CAGGGAAAAG TAAATGGTGA GCCAACCTGG 
GCCCTTCTAC TGACTGCTTT CATAGCAGAG TTAGGAATTC TGATTGCTTC ACTGGACATG 
GTGGCACCAA TCTTATCAAT GTTCTTTCTG ATGTGTTACT TATTTGTTAA TTTGGCATGT 
GCAGTGCAGA CACTCCTGAG AACGCCAAAC TGGAGACCAA GGTTTAAATA TTATCACTGG 
GCACTCTCTT TCTTTGGAAT GAGTATCTGC TTGGCCTTGA TGTTTGTCTG TTCCTGGTAC 
TATGCTTTGA TAGCAATGCT AATCGCTGGA ATGATTTACA AATATATAGA ATACCAAGGA 
GCAGAGAAGG AATGGGGAGA TGGCATCAGA GGTCTGTCTC TAAGTGCAGC CAGATATGCT 
TTACTAAGAC TGGAAGAGGG ACCACCACAC ACCAAAAACT GGAGACCTCA ACTGTTGGTT 
CTTTTAAAGC TAGATGAAGA CCTTCATGTT AAATATCCAC GTCTACTTAC ATTTTCCTCC 
CAGCTAAAAG CTGGCAAAGG TTTAACAATA ATTGGCACCA CCCTTCAAGG AAACTTTTTG 
GAGAGCTATG GAGAGGTTCA GGCTGCTGAG CAGACCATTA AAAACATGAT GGAGATTGAG 
AAAGTCAAAG GATTTTCCCA GGTTGTGGTT TCCAGCGAAG TTTGTGATGG AATTTCACAT 
CTAATTCAGT CATGTGGACT TGGAGGAATG AAGCATAATA CAGTACTGCT GGGATGGCCA 
TATGGTTGGA GGCAGAGTGA AGACCCTCGC TCTTGGAAGA CGTTTATTGA TGCTGTACGC 
TGCACAACTG CAGCTCGCTT GGCACTGATG GTGCCAAAAA ATGTTTCCTT TTACCCCAGC 
AACCATGAAC GCTATCTGGA GGGAAATATT GATGTCTGGT GGATTGTACA TGATGGCGGT 
ATGCTAATGC TCCTGCCTTT TCTACTGAAA CAACACAAGG TCTGGAAAAA GTGCAAAATG 
CGCATTTTTA CTGTGGCTCA GTTGGATGAC AATAGTATCC AAATGAAGAA AGACCTGGCA 
ACATTCCTTT ATCACTTGCG TCTAGAAGCA GAAGTTGAAG TAGTGGAAAT GCACAATAGT 
GACATCTCTG CATACACATA TGAGCGGACA CTGATGATGG AGCAGCGATC ACAGATGCTT 
AGACAGATGC GTCTCACAAA AACAGAAAGG GATAGGGAGG CTCAATTGGT GAAGGATAGA 
AATTCCATCA TTCCTGTTGA GAGTTACTAT TCTGATGAGG AGGATGAAGA GCCAGAATTG 
GAGCTAGCTA AGATACATAT GACTTGGACA AAAGATAAAT ATAATGCTCA ATGGAAAAAC 
AGAAACAATG AAACAATGCA TTTTAGGGAG CTTGCAGATA TAAAGCCGGA CCAGTCTAAT 
GTTCGACGGA TGCACACTGC AGTTAAACTA AATGAGGTCA TAGTGACAAG GTCACATGAT 
GCCAAATTAG TTCTTCTCAA CATGCCAGGT CCCCCCAAAA ATACAGATGG GGATGAAAAC 
TACATGGAAT TTCTTGAAGT ACTCACTGAA GGTTTGGAGA GGGTCCTGTT GGTACGTGGG 
GGAGGACGAG AAGTAATCAC CATCTATTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc12a4 ( XM_002942670.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002942716.1 Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.

Price & Availability↑

CloneID	OXa18989
Clone ID Related Accession (Same CDS sequence)	XM_002942670.4
Accession Version	XM_002942670.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	solute carrier family 12 member 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002942670.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCACT TCACAGTTGT TCCAGTTGAA GAGCAAGCTA GATTGGGATG TGATTACTTG 
GAAGGCCTTA ACGGAGTGGA TTATAGGGAC ACAGGGCACA GTTCTCGGGA TCCCTACGAC 
AGCGTTAGTT CCGACGGTCA TGGAAATTAC AAAGAGAATA GTCCATTTCT TACTGGTTCA 
GAGGCTAATA AAGGAAATCA GTTTTATGAC AAAAATCTGG CCTTATTTGA GGAAGAGCTT 
GATATTCGAC CTAAAGTTTC ATCATTTCTC AGTCGTCTTG TTAACTACAC AAACCTTACC 
CAAGGTGCCA AAGAACATGA AGAGGCTGAG GAAGCTCGAA GAAAAGTTAA CAAATCTCCT 
TGCATGGGAA CAGTAATGGG TGTCTACCTT CCTTGCATGC AAAACATCCT TGGTGTAATC 
TTGTTTCTTC GCCTAACCTG GATTGTCGGC ACTGGTGGAG TACTACAGAC CCTTTTGATT 
ACTCTAATCT GCTGCTGCTG TACTCTGCTT ACAGCCATTT CAATGAGTGC AATAGCTACC 
AATGGAGTTG TGCCAGCTGG TGGATCATAC TTTATGATTT CTCGTTCCCT TGGCCCAGAA 
TTTGGAGGTG CTGTGGGTCT GTGCTTCTAT TTGGGGACTA CATTTGCAGG AGCTATGTAT 
ATTTTGGGAG CTATTGAGAT ACTTCTGATG TATATTGTTC CTGAGGCTGC CATCTTTCAC 
TCTCCAAACA CTCACGAAGA CTCTAGTGCA AAGCTAAACA ATATGCGTGT TTATGGCACA 
GTCTTTCTCA CTTTGATGGC AATCATTGTG TTTGTTGGCG TTAAGTATGT GAACAAATTT 
GCATCTCTCT TCTTGGCTTG TGTGATTATT TCCATCCTGT CAATATATGC TGGATCCATC 
AAATCTATCT TTGATCCTCC TGTTTTTCCG ATTTGCATGC TGGGCAATAG GACTCTTTCC 
AGAGATCGGT TTGAAATTTG TGCCAAAACT ATCATTCTAG ACAATGTAAC AAAGCCTACA 
ACATTATGGG AGCAGTTCTG TGGGTCCTCC AATTTTTCCA CCAGTAGCTG TGATCAATAT 
TTCCTTAAAA ACAATGTAAC TGAGATTGCT GGGATTCCAG GGGCTGCAAG TGGTATTTTA 
AAAGATAATC TGTGGAGTAA TTATTTACAG AAGGGGGAAA TTCTGGAAAA ATCTCAACTT 
TCTTCAGTTG ATGAACTAGG ATCCAAAAGT AATCTCCACC TGTATGTTCT GACAGACATC 
TCTGCTACAT TCACTGTACT TGTGGGCATT TTTTTTCCTT CTGTCACTGG TATCATGGCT 
GGAAGTAATC GGTCAGGGGA TCTGCGGGAT GCCCAGAAAT CCATTCCTGT AGGAACCATA 
CTTGCTATTA TCACCACCTC ATTTATTTAT TTTAGCACTG TTGTTCTGTT TGGCGCTTGC 
ATTGAAGGAG TGGTTTTGCG TGACAAATAT GGAGATGCAG TGAACAAAAA TTTGGTTGTG 
GGGACACTGT CTTGGCCTTC TCCATGGGTT ATTGTCATCG GTTCATTTTT TTCAACTTGT 
GGGGCTGGAT TGCAGAGCTT AACAGGAGCT CCACGGTTAC TACAAGCAAT TGCAAATGAC 
AATATTATTC CATTCCTGAG GGTATTTGGA CAGGGAAAAG TAAATGGTGA GCCAACCTGG 
GCCCTTCTAC TGACTGCTTT CATAGCAGAG TTAGGAATTC TGATTGCTTC ACTGGACATG 
GTGGCACCAA TCTTATCAAT GTTCTTTCTG ATGTGTTACT TATTTGTTAA TTTGGCATGT 
GCAGTGCAGA CACTCCTGAG AACGCCAAAC TGGAGACCAA GGTTTAAATA TTATCACTGG 
GCACTCTCTT TCTTTGGAAT GAGTATCTGC TTGGCCTTGA TGTTTGTCTG TTCCTGGTAC 
TATGCTTTGA TAGCAATGCT AATCGCTGGA ATGATTTACA AATATATAGA ATACCAAGGA 
GCAGAGAAGG AATGGGGAGA TGGCATCAGA GGTCTGTCTC TAAGTGCAGC CAGATATGCT 
TTACTAAGAC TGGAAGAGGG ACCACCACAC ACCAAAAACT GGAGACCTCA ACTGTTGGTT 
CTTTTAAAGC TAGATGAAGA CCTTCATGTT AAATATCCAC GTCTACTTAC ATTTTCCTCC 
CAGCTAAAAG CTGGCAAAGG TTTAACAATA ATTGGCACCA CCCTTCAAGG AAACTTTTTG 
GAGAGCTATG GAGAGGTTCA GGCTGCTGAG CAGACCATTA AAAACATGAT GGAGATTGAG 
AAAGTCAAAG GATTTTCCCA GGTTGTGGTT TCCAGCGAAG TTTGTGATGG AATTTCACAT 
CTAATTCAGT CATGTGGACT TGGAGGAATG AAGCATAATA CAGTACTGCT GGGATGGCCA 
TATGGTTGGA GGCAGAGTGA AGACCCTCGC TCTTGGAAGA CGTTTATTGA TGCTGTACGC 
TGCACAACTG CAGCTCGCTT GGCACTGATG GTGCCAAAAA ATGTTTCCTT TTACCCCAGC 
AACCATGAAC GCTATCTGGA GGGAAATATT GATGTCTGGT GGATTGTACA TGATGGCGGT 
ATGCTAATGC TCCTGCCTTT TCTACTGAAA CAACACAAGG TCTGGAAAAA GTGCAAAATG 
CGCATTTTTA CTGTGGCTCA GTTGGATGAC AATAGTATCC AAATGAAGAA AGACCTGGCA 
ACATTCCTTT ATCACTTGCG TCTAGAAGCA GAAGTTGAAG TAGTGGAAAT GCACAATAGT 
GACATCTCTG CATACACATA TGAGCGGACA CTGATGATGG AGCAGCGATC ACAGATGCTT 
AGACAGATGC GTCTCACAAA AACAGAAAGG GATAGGGAGG CTCAATTGGT GAAGGATAGA 
AATTCCATCA TTCCTGTTGA GAGTTACTAT TCTGATGAGG AGGATGAAGA GCCAGAATTG 
GAGCTAGCTA AGATACATAT GACTTGGACA AAAGATAAAT ATAATGCTCA ATGGAAAAAC 
AGAAACAATG AAACAATGCA TTTTAGGGAG CTTGCAGATA TAAAGCCGGA CCAGTCTAAT 
GTTCGACGGA TGCACACTGC AGTTAAACTA AATGAGGTCA TAGTGACAAG GTCACATGAT 
GCCAAATTAG TTCTTCTCAA CATGCCAGGT CCCCCCAAAA ATACAGATGG GGATGAAAAC 
TACATGGAAT TTCTTGAAGT ACTCACTGAA GGTTTGGAGA GGGTCCTGTT GGTACGTGGG 
GGAGGACGAG AAGTAATCAC CATCTATTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002942716.1
CDS	149..3421
Translation	MPHFTVVPVEEQARLGCDYLEGLNGVDYRDTGHSSRDPYDSVSSDGHGNYKENSPFLTGSEANKGNQFYDKNLALFEEELDIRPKVSSFLSRLVNYTNLTQGAKEHEEAEEARRKVNKSPCMGTVMGVYLPCMQNILGVILFLRLTWIVGTGGVLQTLLITLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGAIEILLMYIVPEAAIFHSPNTHEDSSAKLNNMRVYGTVFLTLMAIIVFVGVKYVNKFASLFLACVIISILSIYAGSIKSIFDPPVFPICMLGNRTLSRDRFEICAKTIILDNVTKPTTLWEQFCGSSNFSTSSCDQYFLKNNVTEIAGIPGAASGILKDNLWSNYLQKGEILEKSQLSSVDELGSKSNLHLYVLTDISATFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIITTSFIYFSTVVLFGACIEGVVLRDKYGDAVNKNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIANDNIIPFLRVFGQGKVNGEPTWALLLTAFIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFFGMSICLALMFVCSWYYALIAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFSSQLKAGKGLTIIGTTLQGNFLESYGEVQAAEQTIKNMMEIEKVKGFSQVVVSSEVCDGISHLIQSCGLGGMKHNTVLLGWPYGWRQSEDPRSWKTFIDAVRCTTAARLALMVPKNVSFYPSNHERYLEGNIDVWWIVHDGGMLMLLPFLLKQHKVWKKCKMRIFTVAQLDDNSIQMKKDLATFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTERDREAQLVKDRNSIIPVESYYSDEEDEEPELELAKIHMTWTKDKYNAQWKNRNNETMHFRELADIKPDQSNVRRMHTAVKLNEVIVTRSHDAKLVLLNMPGPPKNTDGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_002942670.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 4 (slc12a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002942670.4

ORF Insert Sequence:

ATGCCCCACT TCACAGTTGT TCCAGTTGAA GAGCAAGCTA GATTGGGATG TGATTACTTG 
GAAGGCCTTA ACGGAGTGGA TTATAGGGAC ACAGGGCACA GTTCTCGGGA TCCCTACGAC 
AGCGTTAGTT CCGACGGTCA TGGAAATTAC AAAGAGAATA GTCCATTTCT TACTGGTTCA 
GAGGCTAATA AAGGAAATCA GTTTTATGAC AAAAATCTGG CCTTATTTGA GGAAGAGCTT 
GATATTCGAC CTAAAGTTTC ATCATTTCTC AGTCGTCTTG TTAACTACAC AAACCTTACC 
CAAGGTGCCA AAGAACATGA AGAGGCTGAG GAAGCTCGAA GAAAAGTTAA CAAATCTCCT 
TGCATGGGAA CAGTAATGGG TGTCTACCTT CCTTGCATGC AAAACATCCT TGGTGTAATC 
TTGTTTCTTC GCCTAACCTG GATTGTCGGC ACTGGTGGAG TACTACAGAC CCTTTTGATT 
ACTCTAATCT GCTGCTGCTG TACTCTGCTT ACAGCCATTT CAATGAGTGC AATAGCTACC 
AATGGAGTTG TGCCAGCTGG TGGATCATAC TTTATGATTT CTCGTTCCCT TGGCCCAGAA 
TTTGGAGGTG CTGTGGGTCT GTGCTTCTAT TTGGGGACTA CATTTGCAGG AGCTATGTAT 
ATTTTGGGAG CTATTGAGAT ACTTCTGATG TATATTGTTC CTGAGGCTGC CATCTTTCAC 
TCTCCAAACA CTCACGAAGA CTCTAGTGCA AAGCTAAACA ATATGCGTGT TTATGGCACA 
GTCTTTCTCA CTTTGATGGC AATCATTGTG TTTGTTGGCG TTAAGTATGT GAACAAATTT 
GCATCTCTCT TCTTGGCTTG TGTGATTATT TCCATCCTGT CAATATATGC TGGATCCATC 
AAATCTATCT TTGATCCTCC TGTTTTTCCG ATTTGCATGC TGGGCAATAG GACTCTTTCC 
AGAGATCGGT TTGAAATTTG TGCCAAAACT ATCATTCTAG ACAATGTAAC AAAGCCTACA 
ACATTATGGG AGCAGTTCTG TGGGTCCTCC AATTTTTCCA CCAGTAGCTG TGATCAATAT 
TTCCTTAAAA ACAATGTAAC TGAGATTGCT GGGATTCCAG GGGCTGCAAG TGGTATTTTA 
AAAGATAATC TGTGGAGTAA TTATTTACAG AAGGGGGAAA TTCTGGAAAA ATCTCAACTT 
TCTTCAGTTG ATGAACTAGG ATCCAAAAGT AATCTCCACC TGTATGTTCT GACAGACATC 
TCTGCTACAT TCACTGTACT TGTGGGCATT TTTTTTCCTT CTGTCACTGG TATCATGGCT 
GGAAGTAATC GGTCAGGGGA TCTGCGGGAT GCCCAGAAAT CCATTCCTGT AGGAACCATA 
CTTGCTATTA TCACCACCTC ATTTATTTAT TTTAGCACTG TTGTTCTGTT TGGCGCTTGC 
ATTGAAGGAG TGGTTTTGCG TGACAAATAT GGAGATGCAG TGAACAAAAA TTTGGTTGTG 
GGGACACTGT CTTGGCCTTC TCCATGGGTT ATTGTCATCG GTTCATTTTT TTCAACTTGT 
GGGGCTGGAT TGCAGAGCTT AACAGGAGCT CCACGGTTAC TACAAGCAAT TGCAAATGAC 
AATATTATTC CATTCCTGAG GGTATTTGGA CAGGGAAAAG TAAATGGTGA GCCAACCTGG 
GCCCTTCTAC TGACTGCTTT CATAGCAGAG TTAGGAATTC TGATTGCTTC ACTGGACATG 
GTGGCACCAA TCTTATCAAT GTTCTTTCTG ATGTGTTACT TATTTGTTAA TTTGGCATGT 
GCAGTGCAGA CACTCCTGAG AACGCCAAAC TGGAGACCAA GGTTTAAATA TTATCACTGG 
GCACTCTCTT TCTTTGGAAT GAGTATCTGC TTGGCCTTGA TGTTTGTCTG TTCCTGGTAC 
TATGCTTTGA TAGCAATGCT AATCGCTGGA ATGATTTACA AATATATAGA ATACCAAGGA 
GCAGAGAAGG AATGGGGAGA TGGCATCAGA GGTCTGTCTC TAAGTGCAGC CAGATATGCT 
TTACTAAGAC TGGAAGAGGG ACCACCACAC ACCAAAAACT GGAGACCTCA ACTGTTGGTT 
CTTTTAAAGC TAGATGAAGA CCTTCATGTT AAATATCCAC GTCTACTTAC ATTTTCCTCC 
CAGCTAAAAG CTGGCAAAGG TTTAACAATA ATTGGCACCA CCCTTCAAGG AAACTTTTTG 
GAGAGCTATG GAGAGGTTCA GGCTGCTGAG CAGACCATTA AAAACATGAT GGAGATTGAG 
AAAGTCAAAG GATTTTCCCA GGTTGTGGTT TCCAGCGAAG TTTGTGATGG AATTTCACAT 
CTAATTCAGT CATGTGGACT TGGAGGAATG AAGCATAATA CAGTACTGCT GGGATGGCCA 
TATGGTTGGA GGCAGAGTGA AGACCCTCGC TCTTGGAAGA CGTTTATTGA TGCTGTACGC 
TGCACAACTG CAGCTCGCTT GGCACTGATG GTGCCAAAAA ATGTTTCCTT TTACCCCAGC 
AACCATGAAC GCTATCTGGA GGGAAATATT GATGTCTGGT GGATTGTACA TGATGGCGGT 
ATGCTAATGC TCCTGCCTTT TCTACTGAAA CAACACAAGG TCTGGAAAAA GTGCAAAATG 
CGCATTTTTA CTGTGGCTCA GTTGGATGAC AATAGTATCC AAATGAAGAA AGACCTGGCA 
ACATTCCTTT ATCACTTGCG TCTAGAAGCA GAAGTTGAAG TAGTGGAAAT GCACAATAGT 
GACATCTCTG CATACACATA TGAGCGGACA CTGATGATGG AGCAGCGATC ACAGATGCTT 
AGACAGATGC GTCTCACAAA AACAGAAAGG GATAGGGAGG CTCAATTGGT GAAGGATAGA 
AATTCCATCA TTCCTGTTGA GAGTTACTAT TCTGATGAGG AGGATGAAGA GCCAGAATTG 
GAGCTAGCTA AGATACATAT GACTTGGACA AAAGATAAAT ATAATGCTCA ATGGAAAAAC 
AGAAACAATG AAACAATGCA TTTTAGGGAG CTTGCAGATA TAAAGCCGGA CCAGTCTAAT 
GTTCGACGGA TGCACACTGC AGTTAAACTA AATGAGGTCA TAGTGACAAG GTCACATGAT 
GCCAAATTAG TTCTTCTCAA CATGCCAGGT CCCCCCAAAA ATACAGATGG GGATGAAAAC 
TACATGGAAT TTCTTGAAGT ACTCACTGAA GGTTTGGAGA GGGTCCTGTT GGTACGTGGG 
GGAGGACGAG AAGTAATCAC CATCTATTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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slc12a4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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