sema5b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	sema5b
Entrez Gene ID	100493363
Full Name	semaphorin 5B
Synonyms	semag,semg
General protein information	Preferred Names semaphorin 5B Names semaphorin-5B sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012971237.2	XP_012826691.1	semaphorin-5B
XM_031892879.1	XP_031748739.1	LOW QUALITY PROTEIN: semaphorin-5B

Pathways from BioSystems
Gene Ontology

The following sema5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sema5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa35648	XM_012971237.2 Latest version!	Xenopus tropicalis sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (sema5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa66268	XM_031892879.1 Latest version!	Xenopus tropicalis semaphorin 5B (sema5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sema5b ( XM_012971237.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012826691.1 Xenopus tropicalis sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (sema5b), mRNA.

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CloneID	OXa35648
Clone ID Related Accession (Same CDS sequence)	XM_012971237.2
Accession Version	XM_012971237.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	semaphorin-5B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683573.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012971237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCCCC GTTTTACTTG TTGGTGGGGC CCTCTGCCCA AGGGGCCCAA GTGGCCAATC 
CCAGTGAGGC AGCCTGACCC GGGGCCTGAC CCCATCACTT TGCTTTTGCA GATCAGGAAC 
CTGACCGTTC ATGGGGGGCT GGGCCCTTGG TCGTCCTGGC AACCGTGCCA ACACTCCGAC 
GGGGACTCTT CGTGCCTGTG CCGTTCCAGG GTCTGCGACA ACCCCCTGCC GCGCTGCGGG 
GGGCGGGACT GCGACGGGAC GCGGATAGAG ATCGCCAACT GTTCCCGGAA TGGCGGTTGG 
ACTCCATGGT CATCGTGGGC TCAGTGCAGC ACTTCCTGTG GGATTGGGTT CCAAGTTCGG 
CAGAGGACGT GCAGTAACCC CGCCCCTCGC TTTGGGGGAA GAGTCTGCGT GGGGCAGAGC 
CGGGAGGAAC GGTTCTGCAA TGAGAACAGT CCGTGTCCGG TTCCGATCTT CTGGACCTCC 
TGGGGCTCTT GGACTAAGTG CAGCTCAGAC TGCGGGGGGG GGACCCACTC ACGGCAACGA 
TCCTGCGAGA ACGGAAACAC CTGCCCCGGC TGTGCCATGG AATTCCGGCC GTGTAACTCC 
GAGCCCTGCC CCGAAGTGCG CAGAAATACC CCCTGGACGC CGTGGACGCC GGTCAATATC 
ACCCAGGGCG GCGCCCGCCA AGAGCAGCGG TTCCGTTACA CCTGCCGCGC GCAGCTCGCC 
GACCCCCACA ACCTGCAGTT TGGAAGGAAA AAGACGGAGA CGCGGTTCTG CCCCAATGAC 
GGTTCTGCGG CCTGCGAGAC GGACACTCTG GTGGATGATT TGATGCGGAA CAGTAAGACC 
CCCGGCCGTA CGGTCAGCGG GGGGTGGAGC CCATGGAGCG GTTGGTCTTC CTGCTCCCGG 
GAGTGCGAGC TGGGGTTCCG CACCAGGAAA AGAACCTGCA GCAACCCGGA ACCCCGGAAT 
GGAGGCCTTC CCTGTGCCGG GTCGGCCATG GAGTATCAGG ACTGCAATCC CCAGCCCTGC 
CCAGTGAAGG GCTCGTGGTC CTGTTGGTCA CCATGGACCC AGTGCTCAGC CACGTGCGGG 
GGGGGCCATT ACCAACGGAC TCGCACCTGC ACCAACCCCC CACCCTCCAA TGGGGGCGAC 
ATCTGTATTG GGCTCCACAC CGAGGAGGCG CTGTGTAACA CTTACCCCTG TGAAGGAGGT 
TGGGCCGAGT GGTCGGAATG GAGCATGTGT CACGAGGACG GGTCTCAGTA TCGGAACCGA 
TATTGTGAGA TACACAACCC GGGGCCCACC CAGTGCCTGG GGAACAGCAC CCAGTTCCGT 
GACTGTATCT ACAATGAAAT CCCAGTGATC CTGCCGGCTT CCAGCATCGA CGAGAACAGC 
GAGTGCGGAG GATTCAGCCT GATTCACCTT ATTGCCACCG GAGTCTCCTG TTTCTTTGGC 
TCCAGTCTCT TGACCTTTGT GATCTACGTC TACTGCCAGC GGTGCCAGCG CCAGTCCCAG 
GAATCCACCG TCATCCACCC GGCGACCCCC AACCACCTGC ATTATAAGGG GAGCTCCACC 
CCCAAAAACG AGAAGTACAC TCCTATGGAG TTCAAGACGC TCAATAAGAA CAACCTGCTC 
CCAGACGACC GGACCAACTT CTACCCGTTG CAGCAGACCA ACGTTTACAC CACAACTTAC 
TACCCCAGCA CGCTGAGCAA ATACGATTTC AGACCGGACT CCTCTCCGGG CAGAACTTAC 
ACCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012826691.1
CDS	16..1767
Translation	MGPRFTCWWGPLPKGPKWPIPVRQPDPGPDPITLLLQIRNLTVHGGLGPWSSWQPCQHSDGDSSCLCRSRVCDNPLPRCGGRDCDGTRIEIANCSRNGGWTPWSSWAQCSTSCGIGFQVRQRTCSNPAPRFGGRVCVGQSREERFCNENSPCPVPIFWTSWGSWTKCSSDCGGGTHSRQRSCENGNTCPGCAMEFRPCNSEPCPEVRRNTPWTPWTPVNITQGGARQEQRFRYTCRAQLADPHNLQFGRKKTETRFCPNDGSAACETDTLVDDLMRNSKTPGRTVSGGWSPWSGWSSCSRECELGFRTRKRTCSNPEPRNGGLPCAGSAMEYQDCNPQPCPVKGSWSCWSPWTQCSATCGGGHYQRTRTCTNPPPSNGGDICIGLHTEEALCNTYPCEGGWAEWSEWSMCHEDGSQYRNRYCEIHNPGPTQCLGNSTQFRDCIYNEIPVILPASSIDENSECGGFSLIHLIATGVSCFFGSSLLTFVIYVYCQRCQRQSQESTVIHPATPNHLHYKGSSTPKNEKYTPMEFKTLNKNNLLPDDRTNFYPLQQTNVYTTTYYPSTLSKYDFRPDSSPGRTYTNS

Target ORF information:

RefSeq Version	XM_012971237.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B (sema5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012971237.2

ORF Insert Sequence:

ATGGGTCCCC GTTTTACTTG TTGGTGGGGC CCTCTGCCCA AGGGGCCCAA GTGGCCAATC 
CCAGTGAGGC AGCCTGACCC GGGGCCTGAC CCCATCACTT TGCTTTTGCA GATCAGGAAC 
CTGACCGTTC ATGGGGGGCT GGGCCCTTGG TCGTCCTGGC AACCGTGCCA ACACTCCGAC 
GGGGACTCTT CGTGCCTGTG CCGTTCCAGG GTCTGCGACA ACCCCCTGCC GCGCTGCGGG 
GGGCGGGACT GCGACGGGAC GCGGATAGAG ATCGCCAACT GTTCCCGGAA TGGCGGTTGG 
ACTCCATGGT CATCGTGGGC TCAGTGCAGC ACTTCCTGTG GGATTGGGTT CCAAGTTCGG 
CAGAGGACGT GCAGTAACCC CGCCCCTCGC TTTGGGGGAA GAGTCTGCGT GGGGCAGAGC 
CGGGAGGAAC GGTTCTGCAA TGAGAACAGT CCGTGTCCGG TTCCGATCTT CTGGACCTCC 
TGGGGCTCTT GGACTAAGTG CAGCTCAGAC TGCGGGGGGG GGACCCACTC ACGGCAACGA 
TCCTGCGAGA ACGGAAACAC CTGCCCCGGC TGTGCCATGG AATTCCGGCC GTGTAACTCC 
GAGCCCTGCC CCGAAGTGCG CAGAAATACC CCCTGGACGC CGTGGACGCC GGTCAATATC 
ACCCAGGGCG GCGCCCGCCA AGAGCAGCGG TTCCGTTACA CCTGCCGCGC GCAGCTCGCC 
GACCCCCACA ACCTGCAGTT TGGAAGGAAA AAGACGGAGA CGCGGTTCTG CCCCAATGAC 
GGTTCTGCGG CCTGCGAGAC GGACACTCTG GTGGATGATT TGATGCGGAA CAGTAAGACC 
CCCGGCCGTA CGGTCAGCGG GGGGTGGAGC CCATGGAGCG GTTGGTCTTC CTGCTCCCGG 
GAGTGCGAGC TGGGGTTCCG CACCAGGAAA AGAACCTGCA GCAACCCGGA ACCCCGGAAT 
GGAGGCCTTC CCTGTGCCGG GTCGGCCATG GAGTATCAGG ACTGCAATCC CCAGCCCTGC 
CCAGTGAAGG GCTCGTGGTC CTGTTGGTCA CCATGGACCC AGTGCTCAGC CACGTGCGGG 
GGGGGCCATT ACCAACGGAC TCGCACCTGC ACCAACCCCC CACCCTCCAA TGGGGGCGAC 
ATCTGTATTG GGCTCCACAC CGAGGAGGCG CTGTGTAACA CTTACCCCTG TGAAGGAGGT 
TGGGCCGAGT GGTCGGAATG GAGCATGTGT CACGAGGACG GGTCTCAGTA TCGGAACCGA 
TATTGTGAGA TACACAACCC GGGGCCCACC CAGTGCCTGG GGAACAGCAC CCAGTTCCGT 
GACTGTATCT ACAATGAAAT CCCAGTGATC CTGCCGGCTT CCAGCATCGA CGAGAACAGC 
GAGTGCGGAG GATTCAGCCT GATTCACCTT ATTGCCACCG GAGTCTCCTG TTTCTTTGGC 
TCCAGTCTCT TGACCTTTGT GATCTACGTC TACTGCCAGC GGTGCCAGCG CCAGTCCCAG 
GAATCCACCG TCATCCACCC GGCGACCCCC AACCACCTGC ATTATAAGGG GAGCTCCACC 
CCCAAAAACG AGAAGTACAC TCCTATGGAG TTCAAGACGC TCAATAAGAA CAACCTGCTC 
CCAGACGACC GGACCAACTT CTACCCGTTG CAGCAGACCA ACGTTTACAC CACAACTTAC 
TACCCCAGCA CGCTGAGCAA ATACGATTTC AGACCGGACT CCTCTCCGGG CAGAACTTAC 
ACCAACAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sema5b ( XM_031892879.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031748739.1 Xenopus tropicalis semaphorin 5B (sema5b), mRNA.

Price & Availability↑

CloneID	OXa66268
Clone ID Related Accession (Same CDS sequence)	XM_031892879.1
Accession Version	XM_031892879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	LOW QUALITY PROTEIN: semaphorin-5B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels internal stop codons :: corrected 1 genomic stop codons ##RefSeq-Attributes-END##

ATGCTGAGTC GGTGCTGCCG CAGTAGGACC CGTGGCTTCA CCATGTGGCT TCCCCGGCCG 
AGGGCCCTGA CGGCGCTTTC CTTATTCATC TTCCACCTTT CCGCGTCCCA AGTCGCCGTA 
GAGACCCCCG AGAAGGATTA CTGCTCGCGG AGGGAGCACC CCATCCTTTC CTTTCAAGAA 
CTTAAGCCCT GGTTGTCCAA CTTCACCTTC AGCGACGTAC GGGATTTCTC TCAGCTGGCG 
CTGGATCTCA ACCGCAATCA GCTCATCGTA GGCGCCAGGA ATTACCTCTT CCGACTGAGC 
CTCCACAATG TCTCCCTCAT CCAGGCAGCA GAATGGAGTT CGGATGAGGA CACTCAGAGA 
TCTTGTCAGA GCAAAGGCAA AACTGAGGAC GAGTGTCAGA ACTACGTGCG AGTCCTGATC 
GTGAGCGGGA AGCGAGTTTT CACCTGCGGA ACCAACGCCT TCTCCCCGGT TTGCACCAGT 
CGCCAGGTGG GGAACCTGAG CAAGGTGATA GAGAAGATAA ATGGGGTGGC TCGCTGCCCG 
TACGACCCCC GGCACAACTC CACGGCGGTG ATGACCACGC GGGGGGAGCT TTACGCGGCC 
ACGGTTATCG ATTTTTCTGG CCGGGACCCT GTAATCTATC GGAGCTTGGG GAGCGTCCCC 
CCACTGCGCA CCGCCCAGTA CAACTCCAAA TGGCTGAATG AGCCCAACTT TGTGGCCGCC 
TACGACATCG GGATCTTCAC GTATTTCTTC TTCCGGGAGA ACGCGGTGGA GCACGACTGC 
GGGAAGACGG TTTACTCCCG GGTGGCGAGG GTCTGTAAGA ACGACTACGG GGGGCGTTTT 
TTGCTGGAGG ACACCTGGAC CACCTTCATG AAGGCGCGGC TGAACTGCTC CCGCCCCGGC 
GAGATCCCCT TTTACTACAA CGAGTTGCAG AGCACTTTCC TCCTCCCCGA GCAGGACCTC 
ATCTACGGGG TCTTCACCAC CAACGTGAAC AGCATAGCAG CCTCCGCGGT CTGTGCCTTC 
AACCTCAGCG CCATCACCCA GGCCTTCAAT GGGCCCTTCC GGTACCAGGA GAACCCCCGG 
TCCGCTTGGC TGCCAACCAT TAATCCAATA CCCAACTTTC AGTGCGGGAT TCTGAATGAC 
GACAGCCCCA ACGAGAACCT GACGGAGAGG AGCCTGCAGG ACGCCCAGCG CCTCTTCCTC 
ATGAACGACG TGGTGCAGCC GGTGTCGGTG GAGCCGCTGG TGACCCAGGA CAGTGTCCGA 
TTCTCCAAGT TGGTGGTGGA TATCGTCCAG GGCAAAGACA CTTTGTATCA CGTCATGTAC 
ATCGGCACCG AACACGGCAC CATCCTCAAG GCTCTCTCCA CCGCCAACCG GAGCCTCCGC 
AGCTGTTACC TGGAGGAGAT GCACATCCTG CCCATTGGCC AACAGGAGCC AATCAGGAGC 
CTCCAGATCC TGCACAGCAA CAGGTCCCTG TTTGTGGGGC TGAACAATGG GGTCCTGAAG 
GTGCCGCTGG AAAGGTGCTC CATCTACAGG ACGGAAGGCG ACCGCCTGGG GGCCCGGGAC 
CCCTACTGCG GCTGGGACAG TAAGAAGAAG CGCTGCACAC CTTAAGCTTA CCCGCACTAT 
ATCACTGGGC CCAGAAACAC TCTGAGTTGT CCGGTGAGTG GCCTAACTGT ACATGGGGGG 
CGGGGGGCTT GGTGGGTCCG TGCCAAACAC TCCCCGACGG GGCTCTTCGG CCTGTGCCCG 
TTCCCACGGG TCTGCGACAA CCCCCTGCCG CGCTGCGGGG GGCGGGACTG CGACGGGACG 
CGGATAGAGA TCGCCAACTG TTCCCGGAAT GGCGGTTGGA CTCCATGGTC ATCGTGGGCT 
CAGTGCAGCA CTTCCTGTGG GATTGGGTTC CAAGTTCGGC AGCGGACGTG CAGTAACCCC 
GCCCCTCGCT GTNNTGGAAG AGTCTGCGTG GGGCAGAGCC GGGAGGAACG GGTTAAAATG 
ATGCTTGATG GCTTGTTCTG CAATGAGAAC AGTCCGTGTC CGGTTCCGAT CTTCTGGACC 
TCCTGGGGCT CTTGGACTAA GTGCAGCTCA GACTGCGGGG GGGGGACCCA CTCACGGCAA 
CGATCCTGCG AGAACGGAAA CACCTGCCCC GGCTGTGCCA TGGAATTCCG GCCGTGTAAC 
TCCGAGCCCT GCCCCGAAGT GCGCAGAAAT ACCCCCTGGA CGCCGTGGAC GCCGGTCAAT 
ATCACCCAGG GCGGCGCCCG CCAAGAGCAG CGGTTCCGTT ACACCTGCCG CGCGCAGCTC 
GCCGACCCCC ACAACCTGCA GTTTGGAAGG AAAAAGACGG AGACGCGGTT CTGCCCCAAC 
GACGGTTCTG CGGCCTGCGA GACGGACACT CTGGTGGATG ATTTGATGCG GAACAGTAAG 
ACCCCCGGCC GTACGGTCAG CGGGGGGTGG AGCCCATGGA GCGGTTGGTC TTCCTGCTCC 
CGGGAGTGCG AGCTGGGGTT CCGCACCAGG AAAAGAACCT GCAGCAACCC GGAACCCCGG 
AATGGAGGCC TTCCCTGTGC CGGGTCGGCC ATGGAGTATC AGGACTGCAA TCCCCAGCCC 
TGCCCAGTGA AGGGCTCGTG GTCCTGTTGG TCACCGTGGA CCCAGTGCTC AGCCACGTGC 
GGGGGGGGCC ATTACCAACG GACTCGGACC TGCACCAACC CCCCACCCTC CAATGGGGGC 
GACATCTGTA TTGGGCTCCA CACCGAGGAG GCGCTGTGTA ACACTTACCC CTGTGAAGGA 
GGTTGGGCCG AGTGGTCGGA ATGGAGCATG TGTCACGAGG ACGGGTCTCA GTATCGGAAC 
CGATATTGTG AGATACACAA CCCGGGGCCC ACCCAGTGCC TGGGGAACAG CACCCAGTTC 
CGTGACTGTA TCTACAATGA AATCCCAGTG ATCCTGCCGG CTTCCAGCAT TGACGAGAAC 
AGCGAGTGCG GAGGATTCAG CCTGATTCAC CTTATTGCCA CCGGAGTCTC CTGTTTCTTT 
GGCTCCAGTC TCCTGACCTT TGTGATCTAC GTCTACTGCC AGCGGTGCCA GCGCCAGTCC 
CAGGAATCCA CCGTCATCCA CCCGGCGACC CCCAACCACC TGCATTATAA GGGGAGCTCC 
ACCCCCAAAA ACGAGAAGTA CACTCCTATG GAGTTCAAGA CGCTCAATAA GAACAACCTG 
CTCCCAGACG ACCGGACCAA CTTCTACCCG TTGCAGCAGA CCAACGTTTA CACCACAACT 
TACTACCCCA GCACGCTGAG CAAATACGAT TTCAGACCGG ACTCCTCTCC GGGCAGAACT 
TACACCAACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031748739.1
CDS	284..3598
Translation	MLSRCCRSRTRGFTMWLPRPRALTALSLFIFHLSASQVAVETPEKDYCSRREHPILSFQELKPWLSNFTFSDVRDFSQLALDLNRNQLIVGARNYLFRLSLHNVSLIQAAEWSSDEDTQRSCQSKGKTEDECQNYVRVLIVSGKRVFTCGTNAFSPVCTSRQVGNLSKVIEKINGVARCPYDPRHNSTAVMTTRGELYAATVIDFSGRDPVIYRSLGSVPPLRTAQYNSKWLNEPNFVAAYDIGIFTYFFFRENAVEHDCGKTVYSRVARVCKNDYGGRFLLEDTWTTFMKARLNCSRPGEIPFYYNELQSTFLLPEQDLIYGVFTTNVNSIAASAVCAFNLSAITQAFNGPFRYQENPRSAWLPTINPIPNFQCGILNDDSPNENLTERSLQDAQRLFLMNDVVQPVSVEPLVTQDSVRFSKLVVDIVQGKDTLYHVMYIGTEHGTILKALSTANRSLRSCYLEEMHILPIGQQEPIRSLQILHSNRSLFVGLNNGVLKVPLERCSIYRTEGDRLGARDPYCGWDSKKKRCTP*AYPHYITGPRNTLSCPVSGLTVHGGRGAWWVRAKHSPTGLFGLCPFPRVCDNPLPRCGGRDCDGTRIEIANCSRNGGWTPWSSWAQCSTSCGIGFQVRQRTCSNPAPRCnullGRVCVGQSREERVKMMLDGLFCNENSPCPVPIFWTSWGSWTKCSSDCGGGTHSRQRSCENGNTCPGCAMEFRPCNSEPCPEVRRNTPWTPWTPVNITQGGARQEQRFRYTCRAQLADPHNLQFGRKKTETRFCPNDGSAACETDTLVDDLMRNSKTPGRTVSGGWSPWSGWSSCSRECELGFRTRKRTCSNPEPRNGGLPCAGSAMEYQDCNPQPCPVKGSWSCWSPWTQCSATCGGGHYQRTRTCTNPPPSNGGDICIGLHTEEALCNTYPCEGGWAEWSEWSMCHEDGSQYRNRYCEIHNPGPTQCLGNSTQFRDCIYNEIPVILPASSIDENSECGGFSLIHLIATGVSCFFGSSLLTFVIYVYCQRCQRQSQESTVIHPATPNHLHYKGSSTPKNEKYTPMEFKTLNKNNLLPDDRTNFYPLQQTNVYTTTYYPSTLSKYDFRPDSSPGRTYTNS

Target ORF information:

RefSeq Version	XM_031892879.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis semaphorin 5B (sema5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031892879.1

ORF Insert Sequence:

ATGCTGAGTC GGTGCTGCCG CAGTAGGACC CGTGGCTTCA CCATGTGGCT TCCCCGGCCG 
AGGGCCCTGA CGGCGCTTTC CTTATTCATC TTCCACCTTT CCGCGTCCCA AGTCGCCGTA 
GAGACCCCCG AGAAGGATTA CTGCTCGCGG AGGGAGCACC CCATCCTTTC CTTTCAAGAA 
CTTAAGCCCT GGTTGTCCAA CTTCACCTTC AGCGACGTAC GGGATTTCTC TCAGCTGGCG 
CTGGATCTCA ACCGCAATCA GCTCATCGTA GGCGCCAGGA ATTACCTCTT CCGACTGAGC 
CTCCACAATG TCTCCCTCAT CCAGGCAGCA GAATGGAGTT CGGATGAGGA CACTCAGAGA 
TCTTGTCAGA GCAAAGGCAA AACTGAGGAC GAGTGTCAGA ACTACGTGCG AGTCCTGATC 
GTGAGCGGGA AGCGAGTTTT CACCTGCGGA ACCAACGCCT TCTCCCCGGT TTGCACCAGT 
CGCCAGGTGG GGAACCTGAG CAAGGTGATA GAGAAGATAA ATGGGGTGGC TCGCTGCCCG 
TACGACCCCC GGCACAACTC CACGGCGGTG ATGACCACGC GGGGGGAGCT TTACGCGGCC 
ACGGTTATCG ATTTTTCTGG CCGGGACCCT GTAATCTATC GGAGCTTGGG GAGCGTCCCC 
CCACTGCGCA CCGCCCAGTA CAACTCCAAA TGGCTGAATG AGCCCAACTT TGTGGCCGCC 
TACGACATCG GGATCTTCAC GTATTTCTTC TTCCGGGAGA ACGCGGTGGA GCACGACTGC 
GGGAAGACGG TTTACTCCCG GGTGGCGAGG GTCTGTAAGA ACGACTACGG GGGGCGTTTT 
TTGCTGGAGG ACACCTGGAC CACCTTCATG AAGGCGCGGC TGAACTGCTC CCGCCCCGGC 
GAGATCCCCT TTTACTACAA CGAGTTGCAG AGCACTTTCC TCCTCCCCGA GCAGGACCTC 
ATCTACGGGG TCTTCACCAC CAACGTGAAC AGCATAGCAG CCTCCGCGGT CTGTGCCTTC 
AACCTCAGCG CCATCACCCA GGCCTTCAAT GGGCCCTTCC GGTACCAGGA GAACCCCCGG 
TCCGCTTGGC TGCCAACCAT TAATCCAATA CCCAACTTTC AGTGCGGGAT TCTGAATGAC 
GACAGCCCCA ACGAGAACCT GACGGAGAGG AGCCTGCAGG ACGCCCAGCG CCTCTTCCTC 
ATGAACGACG TGGTGCAGCC GGTGTCGGTG GAGCCGCTGG TGACCCAGGA CAGTGTCCGA 
TTCTCCAAGT TGGTGGTGGA TATCGTCCAG GGCAAAGACA CTTTGTATCA CGTCATGTAC 
ATCGGCACCG AACACGGCAC CATCCTCAAG GCTCTCTCCA CCGCCAACCG GAGCCTCCGC 
AGCTGTTACC TGGAGGAGAT GCACATCCTG CCCATTGGCC AACAGGAGCC AATCAGGAGC 
CTCCAGATCC TGCACAGCAA CAGGTCCCTG TTTGTGGGGC TGAACAATGG GGTCCTGAAG 
GTGCCGCTGG AAAGGTGCTC CATCTACAGG ACGGAAGGCG ACCGCCTGGG GGCCCGGGAC 
CCCTACTGCG GCTGGGACAG TAAGAAGAAG CGCTGCACAC CTTAAGCTTA CCCGCACTAT 
ATCACTGGGC CCAGAAACAC TCTGAGTTGT CCGGTGAGTG GCCTAACTGT ACATGGGGGG 
CGGGGGGCTT GGTGGGTCCG TGCCAAACAC TCCCCGACGG GGCTCTTCGG CCTGTGCCCG 
TTCCCACGGG TCTGCGACAA CCCCCTGCCG CGCTGCGGGG GGCGGGACTG CGACGGGACG 
CGGATAGAGA TCGCCAACTG TTCCCGGAAT GGCGGTTGGA CTCCATGGTC ATCGTGGGCT 
CAGTGCAGCA CTTCCTGTGG GATTGGGTTC CAAGTTCGGC AGCGGACGTG CAGTAACCCC 
GCCCCTCGCT GTNNTGGAAG AGTCTGCGTG GGGCAGAGCC GGGAGGAACG GGTTAAAATG 
ATGCTTGATG GCTTGTTCTG CAATGAGAAC AGTCCGTGTC CGGTTCCGAT CTTCTGGACC 
TCCTGGGGCT CTTGGACTAA GTGCAGCTCA GACTGCGGGG GGGGGACCCA CTCACGGCAA 
CGATCCTGCG AGAACGGAAA CACCTGCCCC GGCTGTGCCA TGGAATTCCG GCCGTGTAAC 
TCCGAGCCCT GCCCCGAAGT GCGCAGAAAT ACCCCCTGGA CGCCGTGGAC GCCGGTCAAT 
ATCACCCAGG GCGGCGCCCG CCAAGAGCAG CGGTTCCGTT ACACCTGCCG CGCGCAGCTC 
GCCGACCCCC ACAACCTGCA GTTTGGAAGG AAAAAGACGG AGACGCGGTT CTGCCCCAAC 
GACGGTTCTG CGGCCTGCGA GACGGACACT CTGGTGGATG ATTTGATGCG GAACAGTAAG 
ACCCCCGGCC GTACGGTCAG CGGGGGGTGG AGCCCATGGA GCGGTTGGTC TTCCTGCTCC 
CGGGAGTGCG AGCTGGGGTT CCGCACCAGG AAAAGAACCT GCAGCAACCC GGAACCCCGG 
AATGGAGGCC TTCCCTGTGC CGGGTCGGCC ATGGAGTATC AGGACTGCAA TCCCCAGCCC 
TGCCCAGTGA AGGGCTCGTG GTCCTGTTGG TCACCGTGGA CCCAGTGCTC AGCCACGTGC 
GGGGGGGGCC ATTACCAACG GACTCGGACC TGCACCAACC CCCCACCCTC CAATGGGGGC 
GACATCTGTA TTGGGCTCCA CACCGAGGAG GCGCTGTGTA ACACTTACCC CTGTGAAGGA 
GGTTGGGCCG AGTGGTCGGA ATGGAGCATG TGTCACGAGG ACGGGTCTCA GTATCGGAAC 
CGATATTGTG AGATACACAA CCCGGGGCCC ACCCAGTGCC TGGGGAACAG CACCCAGTTC 
CGTGACTGTA TCTACAATGA AATCCCAGTG ATCCTGCCGG CTTCCAGCAT TGACGAGAAC 
AGCGAGTGCG GAGGATTCAG CCTGATTCAC CTTATTGCCA CCGGAGTCTC CTGTTTCTTT 
GGCTCCAGTC TCCTGACCTT TGTGATCTAC GTCTACTGCC AGCGGTGCCA GCGCCAGTCC 
CAGGAATCCA CCGTCATCCA CCCGGCGACC CCCAACCACC TGCATTATAA GGGGAGCTCC 
ACCCCCAAAA ACGAGAAGTA CACTCCTATG GAGTTCAAGA CGCTCAATAA GAACAACCTG 
CTCCCAGACG ACCGGACCAA CTTCTACCCG TTGCAGCAGA CCAACGTTTA CACCACAACT 
TACTACCCCA GCACGCTGAG CAAATACGAT TTCAGACCGG ACTCCTCTCC GGGCAGAACT 
TACACCAACA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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sema5b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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