efl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following efl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the efl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa55147 XM_004912621.4
Latest version!
Xenopus tropicalis elongation factor like GTPase 1 (efl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa55147 XM_002940100.5
Latest version!
Xenopus tropicalis elongation factor like GTPase 1 (efl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa55147 XM_012959520.3
Latest version!
Xenopus tropicalis elongation factor like GTPase 1 (efl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa07240 XM_004912621.3
Latest version!
Xenopus tropicalis elongation factor like GTPase 1 (efl1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa07240 XM_012959520.2
Latest version!
Xenopus tropicalis elongation factor like GTPase 1 (efl1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa07240 XM_002940100.4
Latest version!
Xenopus tropicalis elongation factor like GTPase 1 (efl1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa55147
    Clone ID Related Accession (Same CDS sequence) XM_002940100.5 , XM_012959520.3 , XM_004912621.4
    Accession Version XM_002940100.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product elongation factor-like GTPase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940100.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGACTTG TCAGTTTAGA GAAAATCGTT ACCCTTCAAA AGAGGGCTGC TAACATTCGG 
    AATATATGTA TATTGGCACA TGTGGACCAT GGTAAAACAA CATTGGCTGA CTGCCTCATA
    TCTAGCAATG GTATAATTTC CAACAGACTG GCAGGAAAGT TGCGATATTT GGACAGCCGT
    GAAGATGAAC AAATCCGAGG AATCACAATG AAATCCAGTG CAATTTCTCT GCATTACAAA
    GATGATGAAG AAGAATATCT TATCAATCTC ATTGACTCAC CTGGGCATGT TGACTTTTCT
    TCAGAGGTCT CAACGGCTGT CCGTCTTTGT GATGGATGCA TTATTGTGGT TGATTCTGTA
    GAAGGAGTCT GTCCTCAGAC ACAGGCAGTA TTACGGCAAG CTTGGCTTGA GAATATCCGT
    CCGGTCCTGG TGATCAATAA AATAGATAGG TTGATAACTG AATTGAAACT TTCCTGTTTA
    GAAGCTTATT CCCATCTCCA AAAGATACTG GAGCAGGTCA ACGCAGTTAC TGGAAGTCTA
    TTCACCTCTA AGGTCCTAGA AGAGAGAGCA GAGAAGGACA TGGCAAGTGA TGTTCCATCT
    GAGACTGTGG ATGGAGATCA GGTGTATGAT TGGAGTGCTG GTCTGGAAGA GACAGATGAT
    TCCCACCTTT ATTTTTCACC AGACCAGGGC AATGTTGTAT TTGCCAGTGC CATAGATGGC
    TGGGGATTTA CCATTGATCA CTTTGCCCAA CTGTACAGTC AGAAAGTTGG TATTAAGGCT
    GCTGTACTTC TGAAGACACT CTGGGGAGAT TACTATCTGA ATACCAAGGC CAAGAAGATC
    ATGAAAGGCG CTCAGAGCAA AGGGAAGAAA CCTTTGTTTG TACAACTCAT CTTGGATAAT
    CTTTGGAGTC TGTATGATGC TGTTATTAAA AGGGAGAAGG AAAAGATAGA TAAAATAGTT
    TTATCCTTGG GGTTAAAAAT CAGCCCTCGG GAATCCCGAC ACTCTGACCC AAAAGTACAT
    GTGAATGCTA TCTGCAGCCA GTGGCTTCCA ATATCACAGG CAGTTCTCTC CATGGTATGC
    AGGAAACTGC CAAGCCCCTT AGATATTCCT GCCGAAAGGG TGGAAAAACT GCTCTGCGTT
    GGGTCCCGAA GGTTTGATTC TCTGCCGCAG GAGACACAGC AACTGAAAGA GACGTTTCTG
    AAGTGTCGGA GTGATGAGAC GGCACCGGTC ATTATTTTTG TTTCCAAAAT GTTTGCCGTT
    GATGCAAAAG CTTTACCTCA GAACAAGCAG AGGCCATTAA CTCAGGAAGA AATTGCACTG
    AGAAGGGAAC TTGCACGGCA GAGGCATGCT GAAAAAATGG CAATCAATCA AGAGCAACTG
    CCGGCAGAGC AGGTTCCCTC TAGCAATGAA TGCGAAATGC CTGCAAACAA AGAGAACACA
    AAAGGGGATA AACAGACATC ACACAGAGAT CCTGAGGTCG TTAAGCCTGA GAAACAAGAA
    GCTGAAAACA AGAGTTATTT TATTGCATTT GCTCGAGTCT TTAGTGGAAT TGTTCGTAGA
    GGCCAAAAGA TCTTTGTTTT GGGTCCAAAA TATGATCCTG CAGAAACATT ATTAAAGTTA
    CCACTAAACT GCACTCCTGC ATGTGACCTG CCTGGCATCC CCCACATGGC GTGCTGCTCT
    CTGGACAATC TTTATCTTCT CATGGGCCGT GAACTGGAAA ATTTAGAAGA AGTTCCTGTA
    GGCAATGTTT TAGGTATTGG AGGTTTGGAA GAATTTGTTC TAAAGTCAGC AACACTGTCC
    ACTTCACCAG CTTGTCCACC CTTTATTCCT CTTAATTTTG AAGCAACTCC AATTGTCCGG
    GTAGCTGTTG AACCAAAGCA TCCAAGTGAA ATGCCCCAGC TGGTAAAAGG AATGAAGCTT
    TTAAACCAGG CTGACCCTTG CGTGGAGGTT TTAATACAAG AAACTGGAGA GCATGTCCTG
    ATCACTGCTG GAGAAGTTCA CCTACAGCGC TGCTTGGATG ATTTAAGAGA GAGGTTTGCT
    AAGATTCAGG TCAGTTCGTC TGCTCCTATA ATACCGTTTA GAGAAACTAT CATAAGGCCA
    CCTAAAGTTG ATATGGTGAA TGAAGAGATT GGAAAGCAAC AGAAGATTGC GGTCATACAT
    CAAGTCAAAG AAGAGCAGAG CAAGTGTCCT GAAGGAGTCC AGGTTGACCC TGATGGCTTA
    GTCACCTTGA CTACACCAAA TAAGCTAGCT ACACTGAGTG TCAGGGCCAT GCCCCTTCCA
    GAAGAAGTGA CGCAGCTTCT GGAAAAAAAC AGTGATTTAA TAAGAACAAT GGAGCAAATA
    AATATGGCTT TAAATGAAGG TAGCTACACC ATTCATTTTA ATGAGTCAGC CATTGAAAGG
    ATAACCGCAT TCAAAAGTAA TCTCCAGCAA CTCCTGCAAG GAAGAAGGTG GAGGAATGCT
    GTGGACCAGA TATGGTCCTT TGGCCCAAGA CGTTATGGCC CAAATATCTT ATTGAACCGA
    ATTGAAGGTT ATGACAGGCC ATCAGTTTGG CAGTGCCTTG AAAAAAGTAT TAGAGAGGGG
    AAATATAGAA ATTTTGACAA CAGCATTGTT AGTGGATTTC AGCTAGCAAC TCTTGCTGGC
    CCCATGTGCG AGGAACCACT AATGGGTGTG TGCTTTATTG TGGAAAAGCT GGATCTGAGC
    AGGCCTTTGC TTGCTAAAGA TAGTGCTAGC CAAGATTCAG AAGTGGATAC CAGAACAGAG
    CCAGAACATA GTGAAACTGG GACAACTGGG ACAACTGGGG CCTTAATCAA TAATATCTCA
    ACATCAGCCA CTGAGGAAAC AGAGTTAATT CCTGAAAATG GTGCAAGACC TTTCAGCAAA
    CGAAAAGGTG ACTTATTGAC AGATTGCTAT GGGCCTTTTT CTGGACAACT TATAGCCACT
    ATGAAGGAAG CTTGTCGTTA TGCATTCCAA GTTAAACCCC AGAGACTCAT GGCTGCCATG
    TACACGTGTG AAATAATGGC AACTGCTGAA GTGTTAGGTC GAGTCTATGG TGTCCTGTCA
    AAAAGAGAAG GCCGTGTTCT GTTGGAAGAG ATGAAAGAAG GAACAGACAT GTTCATTATC
    AAGGCAGTGC TGCCGGTGGC TGAAAGCTTT GGCTTTGCTG ATGAAATAAG AAAGAGAACA
    AGTGGCCTGG CCAGCCCACA GCTGGTGTTC AGCCACTGGG AGGTGATTTC TAGTGATCCA
    TTCTGGGTAC CAACCACAGA GGAAGAATAC TTGCACTTTG GAGAAAAGGC AGACTCTGAA
    AACCAGGCCA GAAAATACAT GAACTCTGTG AGGAAGAGGA AAGGACTTTA TGTAGAAGAA
    AAGATTGTGG AACATGCCGA GAAACAGAGA ACTCTTGGCA AGAACAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002940146.3
    CDS101..3511
    Translation

    Target ORF information:

    RefSeq Version XM_002940100.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis elongation factor like GTPase 1 (efl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002940100.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGACTTG TCAGTTTAGA GAAAATCGTT ACCCTTCAAA AGAGGGCTGC TAACATTCGG 
    AATATATGTA TATTGGCACA TGTGGACCAT GGTAAAACAA CATTGGCTGA CTGCCTCATA
    TCTAGCAATG GTATAATTTC CAACAGACTG GCAGGAAAGT TGCGATATTT GGACAGCCGT
    GAAGATGAAC AAATCCGAGG AATCACAATG AAATCCAGTG CAATTTCTCT GCATTACAAA
    GATGATGAAG AAGAATATCT TATCAATCTC ATTGACTCAC CTGGGCATGT TGACTTTTCT
    TCAGAGGTCT CAACGGCTGT CCGTCTTTGT GATGGATGCA TTATTGTGGT TGATTCTGTA
    GAAGGAGTCT GTCCTCAGAC ACAGGCAGTA TTACGGCAAG CTTGGCTTGA GAATATCCGT
    CCGGTCCTGG TGATCAATAA AATAGATAGG TTGATAACTG AATTGAAACT TTCCTGTTTA
    GAAGCTTATT CCCATCTCCA AAAGATACTG GAGCAGGTCA ACGCAGTTAC TGGAAGTCTA
    TTCACCTCTA AGGTCCTAGA AGAGAGAGCA GAGAAGGACA TGGCAAGTGA TGTTCCATCT
    GAGACTGTGG ATGGAGATCA GGTGTATGAT TGGAGTGCTG GTCTGGAAGA GACAGATGAT
    TCCCACCTTT ATTTTTCACC AGACCAGGGC AATGTTGTAT TTGCCAGTGC CATAGATGGC
    TGGGGATTTA CCATTGATCA CTTTGCCCAA CTGTACAGTC AGAAAGTTGG TATTAAGGCT
    GCTGTACTTC TGAAGACACT CTGGGGAGAT TACTATCTGA ATACCAAGGC CAAGAAGATC
    ATGAAAGGCG CTCAGAGCAA AGGGAAGAAA CCTTTGTTTG TACAACTCAT CTTGGATAAT
    CTTTGGAGTC TGTATGATGC TGTTATTAAA AGGGAGAAGG AAAAGATAGA TAAAATAGTT
    TTATCCTTGG GGTTAAAAAT CAGCCCTCGG GAATCCCGAC ACTCTGACCC AAAAGTACAT
    GTGAATGCTA TCTGCAGCCA GTGGCTTCCA ATATCACAGG CAGTTCTCTC CATGGTATGC
    AGGAAACTGC CAAGCCCCTT AGATATTCCT GCCGAAAGGG TGGAAAAACT GCTCTGCGTT
    GGGTCCCGAA GGTTTGATTC TCTGCCGCAG GAGACACAGC AACTGAAAGA GACGTTTCTG
    AAGTGTCGGA GTGATGAGAC GGCACCGGTC ATTATTTTTG TTTCCAAAAT GTTTGCCGTT
    GATGCAAAAG CTTTACCTCA GAACAAGCAG AGGCCATTAA CTCAGGAAGA AATTGCACTG
    AGAAGGGAAC TTGCACGGCA GAGGCATGCT GAAAAAATGG CAATCAATCA AGAGCAACTG
    CCGGCAGAGC AGGTTCCCTC TAGCAATGAA TGCGAAATGC CTGCAAACAA AGAGAACACA
    AAAGGGGATA AACAGACATC ACACAGAGAT CCTGAGGTCG TTAAGCCTGA GAAACAAGAA
    GCTGAAAACA AGAGTTATTT TATTGCATTT GCTCGAGTCT TTAGTGGAAT TGTTCGTAGA
    GGCCAAAAGA TCTTTGTTTT GGGTCCAAAA TATGATCCTG CAGAAACATT ATTAAAGTTA
    CCACTAAACT GCACTCCTGC ATGTGACCTG CCTGGCATCC CCCACATGGC GTGCTGCTCT
    CTGGACAATC TTTATCTTCT CATGGGCCGT GAACTGGAAA ATTTAGAAGA AGTTCCTGTA
    GGCAATGTTT TAGGTATTGG AGGTTTGGAA GAATTTGTTC TAAAGTCAGC AACACTGTCC
    ACTTCACCAG CTTGTCCACC CTTTATTCCT CTTAATTTTG AAGCAACTCC AATTGTCCGG
    GTAGCTGTTG AACCAAAGCA TCCAAGTGAA ATGCCCCAGC TGGTAAAAGG AATGAAGCTT
    TTAAACCAGG CTGACCCTTG CGTGGAGGTT TTAATACAAG AAACTGGAGA GCATGTCCTG
    ATCACTGCTG GAGAAGTTCA CCTACAGCGC TGCTTGGATG ATTTAAGAGA GAGGTTTGCT
    AAGATTCAGG TCAGTTCGTC TGCTCCTATA ATACCGTTTA GAGAAACTAT CATAAGGCCA
    CCTAAAGTTG ATATGGTGAA TGAAGAGATT GGAAAGCAAC AGAAGATTGC GGTCATACAT
    CAAGTCAAAG AAGAGCAGAG CAAGTGTCCT GAAGGAGTCC AGGTTGACCC TGATGGCTTA
    GTCACCTTGA CTACACCAAA TAAGCTAGCT ACACTGAGTG TCAGGGCCAT GCCCCTTCCA
    GAAGAAGTGA CGCAGCTTCT GGAAAAAAAC AGTGATTTAA TAAGAACAAT GGAGCAAATA
    AATATGGCTT TAAATGAAGG TAGCTACACC ATTCATTTTA ATGAGTCAGC CATTGAAAGG
    ATAACCGCAT TCAAAAGTAA TCTCCAGCAA CTCCTGCAAG GAAGAAGGTG GAGGAATGCT
    GTGGACCAGA TATGGTCCTT TGGCCCAAGA CGTTATGGCC CAAATATCTT ATTGAACCGA
    ATTGAAGGTT ATGACAGGCC ATCAGTTTGG CAGTGCCTTG AAAAAAGTAT TAGAGAGGGG
    AAATATAGAA ATTTTGACAA CAGCATTGTT AGTGGATTTC AGCTAGCAAC TCTTGCTGGC
    CCCATGTGCG AGGAACCACT AATGGGTGTG TGCTTTATTG TGGAAAAGCT GGATCTGAGC
    AGGCCTTTGC TTGCTAAAGA TAGTGCTAGC CAAGATTCAG AAGTGGATAC CAGAACAGAG
    CCAGAACATA GTGAAACTGG GACAACTGGG ACAACTGGGG CCTTAATCAA TAATATCTCA
    ACATCAGCCA CTGAGGAAAC AGAGTTAATT CCTGAAAATG GTGCAAGACC TTTCAGCAAA
    CGAAAAGGTG ACTTATTGAC AGATTGCTAT GGGCCTTTTT CTGGACAACT TATAGCCACT
    ATGAAGGAAG CTTGTCGTTA TGCATTCCAA GTTAAACCCC AGAGACTCAT GGCTGCCATG
    TACACGTGTG AAATAATGGC AACTGCTGAA GTGTTAGGTC GAGTCTATGG TGTCCTGTCA
    AAAAGAGAAG GCCGTGTTCT GTTGGAAGAG ATGAAAGAAG GAACAGACAT GTTCATTATC
    AAGGCAGTGC TGCCGGTGGC TGAAAGCTTT GGCTTTGCTG ATGAAATAAG AAAGAGAACA
    AGTGGCCTGG CCAGCCCACA GCTGGTGTTC AGCCACTGGG AGGTGATTTC TAGTGATCCA
    TTCTGGGTAC CAACCACAGA GGAAGAATAC TTGCACTTTG GAGAAAAGGC AGACTCTGAA
    AACCAGGCCA GAAAATACAT GAACTCTGTG AGGAAGAGGA AAGGACTTTA TGTAGAAGAA
    AAGATTGTGG AACATGCCGA GAAACAGAGA ACTCTTGGCA AGAACAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55147
    Clone ID Related Accession (Same CDS sequence) XM_002940100.5 , XM_012959520.3 , XM_004912621.4
    Accession Version XM_012959520.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product elongation factor-like GTPase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959520.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGACTTG TCAGTTTAGA GAAAATCGTT ACCCTTCAAA AGAGGGCTGC TAACATTCGG 
    AATATATGTA TATTGGCACA TGTGGACCAT GGTAAAACAA CATTGGCTGA CTGCCTCATA
    TCTAGCAATG GTATAATTTC CAACAGACTG GCAGGAAAGT TGCGATATTT GGACAGCCGT
    GAAGATGAAC AAATCCGAGG AATCACAATG AAATCCAGTG CAATTTCTCT GCATTACAAA
    GATGATGAAG AAGAATATCT TATCAATCTC ATTGACTCAC CTGGGCATGT TGACTTTTCT
    TCAGAGGTCT CAACGGCTGT CCGTCTTTGT GATGGATGCA TTATTGTGGT TGATTCTGTA
    GAAGGAGTCT GTCCTCAGAC ACAGGCAGTA TTACGGCAAG CTTGGCTTGA GAATATCCGT
    CCGGTCCTGG TGATCAATAA AATAGATAGG TTGATAACTG AATTGAAACT TTCCTGTTTA
    GAAGCTTATT CCCATCTCCA AAAGATACTG GAGCAGGTCA ACGCAGTTAC TGGAAGTCTA
    TTCACCTCTA AGGTCCTAGA AGAGAGAGCA GAGAAGGACA TGGCAAGTGA TGTTCCATCT
    GAGACTGTGG ATGGAGATCA GGTGTATGAT TGGAGTGCTG GTCTGGAAGA GACAGATGAT
    TCCCACCTTT ATTTTTCACC AGACCAGGGC AATGTTGTAT TTGCCAGTGC CATAGATGGC
    TGGGGATTTA CCATTGATCA CTTTGCCCAA CTGTACAGTC AGAAAGTTGG TATTAAGGCT
    GCTGTACTTC TGAAGACACT CTGGGGAGAT TACTATCTGA ATACCAAGGC CAAGAAGATC
    ATGAAAGGCG CTCAGAGCAA AGGGAAGAAA CCTTTGTTTG TACAACTCAT CTTGGATAAT
    CTTTGGAGTC TGTATGATGC TGTTATTAAA AGGGAGAAGG AAAAGATAGA TAAAATAGTT
    TTATCCTTGG GGTTAAAAAT CAGCCCTCGG GAATCCCGAC ACTCTGACCC AAAAGTACAT
    GTGAATGCTA TCTGCAGCCA GTGGCTTCCA ATATCACAGG CAGTTCTCTC CATGGTATGC
    AGGAAACTGC CAAGCCCCTT AGATATTCCT GCCGAAAGGG TGGAAAAACT GCTCTGCGTT
    GGGTCCCGAA GGTTTGATTC TCTGCCGCAG GAGACACAGC AACTGAAAGA GACGTTTCTG
    AAGTGTCGGA GTGATGAGAC GGCACCGGTC ATTATTTTTG TTTCCAAAAT GTTTGCCGTT
    GATGCAAAAG CTTTACCTCA GAACAAGCAG AGGCCATTAA CTCAGGAAGA AATTGCACTG
    AGAAGGGAAC TTGCACGGCA GAGGCATGCT GAAAAAATGG CAATCAATCA AGAGCAACTG
    CCGGCAGAGC AGGTTCCCTC TAGCAATGAA TGCGAAATGC CTGCAAACAA AGAGAACACA
    AAAGGGGATA AACAGACATC ACACAGAGAT CCTGAGGTCG TTAAGCCTGA GAAACAAGAA
    GCTGAAAACA AGAGTTATTT TATTGCATTT GCTCGAGTCT TTAGTGGAAT TGTTCGTAGA
    GGCCAAAAGA TCTTTGTTTT GGGTCCAAAA TATGATCCTG CAGAAACATT ATTAAAGTTA
    CCACTAAACT GCACTCCTGC ATGTGACCTG CCTGGCATCC CCCACATGGC GTGCTGCTCT
    CTGGACAATC TTTATCTTCT CATGGGCCGT GAACTGGAAA ATTTAGAAGA AGTTCCTGTA
    GGCAATGTTT TAGGTATTGG AGGTTTGGAA GAATTTGTTC TAAAGTCAGC AACACTGTCC
    ACTTCACCAG CTTGTCCACC CTTTATTCCT CTTAATTTTG AAGCAACTCC AATTGTCCGG
    GTAGCTGTTG AACCAAAGCA TCCAAGTGAA ATGCCCCAGC TGGTAAAAGG AATGAAGCTT
    TTAAACCAGG CTGACCCTTG CGTGGAGGTT TTAATACAAG AAACTGGAGA GCATGTCCTG
    ATCACTGCTG GAGAAGTTCA CCTACAGCGC TGCTTGGATG ATTTAAGAGA GAGGTTTGCT
    AAGATTCAGG TCAGTTCGTC TGCTCCTATA ATACCGTTTA GAGAAACTAT CATAAGGCCA
    CCTAAAGTTG ATATGGTGAA TGAAGAGATT GGAAAGCAAC AGAAGATTGC GGTCATACAT
    CAAGTCAAAG AAGAGCAGAG CAAGTGTCCT GAAGGAGTCC AGGTTGACCC TGATGGCTTA
    GTCACCTTGA CTACACCAAA TAAGCTAGCT ACACTGAGTG TCAGGGCCAT GCCCCTTCCA
    GAAGAAGTGA CGCAGCTTCT GGAAAAAAAC AGTGATTTAA TAAGAACAAT GGAGCAAATA
    AATATGGCTT TAAATGAAGG TAGCTACACC ATTCATTTTA ATGAGTCAGC CATTGAAAGG
    ATAACCGCAT TCAAAAGTAA TCTCCAGCAA CTCCTGCAAG GAAGAAGGTG GAGGAATGCT
    GTGGACCAGA TATGGTCCTT TGGCCCAAGA CGTTATGGCC CAAATATCTT ATTGAACCGA
    ATTGAAGGTT ATGACAGGCC ATCAGTTTGG CAGTGCCTTG AAAAAAGTAT TAGAGAGGGG
    AAATATAGAA ATTTTGACAA CAGCATTGTT AGTGGATTTC AGCTAGCAAC TCTTGCTGGC
    CCCATGTGCG AGGAACCACT AATGGGTGTG TGCTTTATTG TGGAAAAGCT GGATCTGAGC
    AGGCCTTTGC TTGCTAAAGA TAGTGCTAGC CAAGATTCAG AAGTGGATAC CAGAACAGAG
    CCAGAACATA GTGAAACTGG GACAACTGGG ACAACTGGGG CCTTAATCAA TAATATCTCA
    ACATCAGCCA CTGAGGAAAC AGAGTTAATT CCTGAAAATG GTGCAAGACC TTTCAGCAAA
    CGAAAAGGTG ACTTATTGAC AGATTGCTAT GGGCCTTTTT CTGGACAACT TATAGCCACT
    ATGAAGGAAG CTTGTCGTTA TGCATTCCAA GTTAAACCCC AGAGACTCAT GGCTGCCATG
    TACACGTGTG AAATAATGGC AACTGCTGAA GTGTTAGGTC GAGTCTATGG TGTCCTGTCA
    AAAAGAGAAG GCCGTGTTCT GTTGGAAGAG ATGAAAGAAG GAACAGACAT GTTCATTATC
    AAGGCAGTGC TGCCGGTGGC TGAAAGCTTT GGCTTTGCTG ATGAAATAAG AAAGAGAACA
    AGTGGCCTGG CCAGCCCACA GCTGGTGTTC AGCCACTGGG AGGTGATTTC TAGTGATCCA
    TTCTGGGTAC CAACCACAGA GGAAGAATAC TTGCACTTTG GAGAAAAGGC AGACTCTGAA
    AACCAGGCCA GAAAATACAT GAACTCTGTG AGGAAGAGGA AAGGACTTTA TGTAGAAGAA
    AAGATTGTGG AACATGCCGA GAAACAGAGA ACTCTTGGCA AGAACAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814974.2
    CDS29..3439
    Translation

    Target ORF information:

    RefSeq Version XM_012959520.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis elongation factor like GTPase 1 (efl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959520.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGACTTG TCAGTTTAGA GAAAATCGTT ACCCTTCAAA AGAGGGCTGC TAACATTCGG 
    AATATATGTA TATTGGCACA TGTGGACCAT GGTAAAACAA CATTGGCTGA CTGCCTCATA
    TCTAGCAATG GTATAATTTC CAACAGACTG GCAGGAAAGT TGCGATATTT GGACAGCCGT
    GAAGATGAAC AAATCCGAGG AATCACAATG AAATCCAGTG CAATTTCTCT GCATTACAAA
    GATGATGAAG AAGAATATCT TATCAATCTC ATTGACTCAC CTGGGCATGT TGACTTTTCT
    TCAGAGGTCT CAACGGCTGT CCGTCTTTGT GATGGATGCA TTATTGTGGT TGATTCTGTA
    GAAGGAGTCT GTCCTCAGAC ACAGGCAGTA TTACGGCAAG CTTGGCTTGA GAATATCCGT
    CCGGTCCTGG TGATCAATAA AATAGATAGG TTGATAACTG AATTGAAACT TTCCTGTTTA
    GAAGCTTATT CCCATCTCCA AAAGATACTG GAGCAGGTCA ACGCAGTTAC TGGAAGTCTA
    TTCACCTCTA AGGTCCTAGA AGAGAGAGCA GAGAAGGACA TGGCAAGTGA TGTTCCATCT
    GAGACTGTGG ATGGAGATCA GGTGTATGAT TGGAGTGCTG GTCTGGAAGA GACAGATGAT
    TCCCACCTTT ATTTTTCACC AGACCAGGGC AATGTTGTAT TTGCCAGTGC CATAGATGGC
    TGGGGATTTA CCATTGATCA CTTTGCCCAA CTGTACAGTC AGAAAGTTGG TATTAAGGCT
    GCTGTACTTC TGAAGACACT CTGGGGAGAT TACTATCTGA ATACCAAGGC CAAGAAGATC
    ATGAAAGGCG CTCAGAGCAA AGGGAAGAAA CCTTTGTTTG TACAACTCAT CTTGGATAAT
    CTTTGGAGTC TGTATGATGC TGTTATTAAA AGGGAGAAGG AAAAGATAGA TAAAATAGTT
    TTATCCTTGG GGTTAAAAAT CAGCCCTCGG GAATCCCGAC ACTCTGACCC AAAAGTACAT
    GTGAATGCTA TCTGCAGCCA GTGGCTTCCA ATATCACAGG CAGTTCTCTC CATGGTATGC
    AGGAAACTGC CAAGCCCCTT AGATATTCCT GCCGAAAGGG TGGAAAAACT GCTCTGCGTT
    GGGTCCCGAA GGTTTGATTC TCTGCCGCAG GAGACACAGC AACTGAAAGA GACGTTTCTG
    AAGTGTCGGA GTGATGAGAC GGCACCGGTC ATTATTTTTG TTTCCAAAAT GTTTGCCGTT
    GATGCAAAAG CTTTACCTCA GAACAAGCAG AGGCCATTAA CTCAGGAAGA AATTGCACTG
    AGAAGGGAAC TTGCACGGCA GAGGCATGCT GAAAAAATGG CAATCAATCA AGAGCAACTG
    CCGGCAGAGC AGGTTCCCTC TAGCAATGAA TGCGAAATGC CTGCAAACAA AGAGAACACA
    AAAGGGGATA AACAGACATC ACACAGAGAT CCTGAGGTCG TTAAGCCTGA GAAACAAGAA
    GCTGAAAACA AGAGTTATTT TATTGCATTT GCTCGAGTCT TTAGTGGAAT TGTTCGTAGA
    GGCCAAAAGA TCTTTGTTTT GGGTCCAAAA TATGATCCTG CAGAAACATT ATTAAAGTTA
    CCACTAAACT GCACTCCTGC ATGTGACCTG CCTGGCATCC CCCACATGGC GTGCTGCTCT
    CTGGACAATC TTTATCTTCT CATGGGCCGT GAACTGGAAA ATTTAGAAGA AGTTCCTGTA
    GGCAATGTTT TAGGTATTGG AGGTTTGGAA GAATTTGTTC TAAAGTCAGC AACACTGTCC
    ACTTCACCAG CTTGTCCACC CTTTATTCCT CTTAATTTTG AAGCAACTCC AATTGTCCGG
    GTAGCTGTTG AACCAAAGCA TCCAAGTGAA ATGCCCCAGC TGGTAAAAGG AATGAAGCTT
    TTAAACCAGG CTGACCCTTG CGTGGAGGTT TTAATACAAG AAACTGGAGA GCATGTCCTG
    ATCACTGCTG GAGAAGTTCA CCTACAGCGC TGCTTGGATG ATTTAAGAGA GAGGTTTGCT
    AAGATTCAGG TCAGTTCGTC TGCTCCTATA ATACCGTTTA GAGAAACTAT CATAAGGCCA
    CCTAAAGTTG ATATGGTGAA TGAAGAGATT GGAAAGCAAC AGAAGATTGC GGTCATACAT
    CAAGTCAAAG AAGAGCAGAG CAAGTGTCCT GAAGGAGTCC AGGTTGACCC TGATGGCTTA
    GTCACCTTGA CTACACCAAA TAAGCTAGCT ACACTGAGTG TCAGGGCCAT GCCCCTTCCA
    GAAGAAGTGA CGCAGCTTCT GGAAAAAAAC AGTGATTTAA TAAGAACAAT GGAGCAAATA
    AATATGGCTT TAAATGAAGG TAGCTACACC ATTCATTTTA ATGAGTCAGC CATTGAAAGG
    ATAACCGCAT TCAAAAGTAA TCTCCAGCAA CTCCTGCAAG GAAGAAGGTG GAGGAATGCT
    GTGGACCAGA TATGGTCCTT TGGCCCAAGA CGTTATGGCC CAAATATCTT ATTGAACCGA
    ATTGAAGGTT ATGACAGGCC ATCAGTTTGG CAGTGCCTTG AAAAAAGTAT TAGAGAGGGG
    AAATATAGAA ATTTTGACAA CAGCATTGTT AGTGGATTTC AGCTAGCAAC TCTTGCTGGC
    CCCATGTGCG AGGAACCACT AATGGGTGTG TGCTTTATTG TGGAAAAGCT GGATCTGAGC
    AGGCCTTTGC TTGCTAAAGA TAGTGCTAGC CAAGATTCAG AAGTGGATAC CAGAACAGAG
    CCAGAACATA GTGAAACTGG GACAACTGGG ACAACTGGGG CCTTAATCAA TAATATCTCA
    ACATCAGCCA CTGAGGAAAC AGAGTTAATT CCTGAAAATG GTGCAAGACC TTTCAGCAAA
    CGAAAAGGTG ACTTATTGAC AGATTGCTAT GGGCCTTTTT CTGGACAACT TATAGCCACT
    ATGAAGGAAG CTTGTCGTTA TGCATTCCAA GTTAAACCCC AGAGACTCAT GGCTGCCATG
    TACACGTGTG AAATAATGGC AACTGCTGAA GTGTTAGGTC GAGTCTATGG TGTCCTGTCA
    AAAAGAGAAG GCCGTGTTCT GTTGGAAGAG ATGAAAGAAG GAACAGACAT GTTCATTATC
    AAGGCAGTGC TGCCGGTGGC TGAAAGCTTT GGCTTTGCTG ATGAAATAAG AAAGAGAACA
    AGTGGCCTGG CCAGCCCACA GCTGGTGTTC AGCCACTGGG AGGTGATTTC TAGTGATCCA
    TTCTGGGTAC CAACCACAGA GGAAGAATAC TTGCACTTTG GAGAAAAGGC AGACTCTGAA
    AACCAGGCCA GAAAATACAT GAACTCTGTG AGGAAGAGGA AAGGACTTTA TGTAGAAGAA
    AAGATTGTGG AACATGCCGA GAAACAGAGA ACTCTTGGCA AGAACAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa55147
    Clone ID Related Accession (Same CDS sequence) XM_002940100.5 , XM_012959520.3 , XM_004912621.4
    Accession Version XM_004912621.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product elongation factor-like GTPase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004912621.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGACTTG TCAGTTTAGA GAAAATCGTT ACCCTTCAAA AGAGGGCTGC TAACATTCGG 
    AATATATGTA TATTGGCACA TGTGGACCAT GGTAAAACAA CATTGGCTGA CTGCCTCATA
    TCTAGCAATG GTATAATTTC CAACAGACTG GCAGGAAAGT TGCGATATTT GGACAGCCGT
    GAAGATGAAC AAATCCGAGG AATCACAATG AAATCCAGTG CAATTTCTCT GCATTACAAA
    GATGATGAAG AAGAATATCT TATCAATCTC ATTGACTCAC CTGGGCATGT TGACTTTTCT
    TCAGAGGTCT CAACGGCTGT CCGTCTTTGT GATGGATGCA TTATTGTGGT TGATTCTGTA
    GAAGGAGTCT GTCCTCAGAC ACAGGCAGTA TTACGGCAAG CTTGGCTTGA GAATATCCGT
    CCGGTCCTGG TGATCAATAA AATAGATAGG TTGATAACTG AATTGAAACT TTCCTGTTTA
    GAAGCTTATT CCCATCTCCA AAAGATACTG GAGCAGGTCA ACGCAGTTAC TGGAAGTCTA
    TTCACCTCTA AGGTCCTAGA AGAGAGAGCA GAGAAGGACA TGGCAAGTGA TGTTCCATCT
    GAGACTGTGG ATGGAGATCA GGTGTATGAT TGGAGTGCTG GTCTGGAAGA GACAGATGAT
    TCCCACCTTT ATTTTTCACC AGACCAGGGC AATGTTGTAT TTGCCAGTGC CATAGATGGC
    TGGGGATTTA CCATTGATCA CTTTGCCCAA CTGTACAGTC AGAAAGTTGG TATTAAGGCT
    GCTGTACTTC TGAAGACACT CTGGGGAGAT TACTATCTGA ATACCAAGGC CAAGAAGATC
    ATGAAAGGCG CTCAGAGCAA AGGGAAGAAA CCTTTGTTTG TACAACTCAT CTTGGATAAT
    CTTTGGAGTC TGTATGATGC TGTTATTAAA AGGGAGAAGG AAAAGATAGA TAAAATAGTT
    TTATCCTTGG GGTTAAAAAT CAGCCCTCGG GAATCCCGAC ACTCTGACCC AAAAGTACAT
    GTGAATGCTA TCTGCAGCCA GTGGCTTCCA ATATCACAGG CAGTTCTCTC CATGGTATGC
    AGGAAACTGC CAAGCCCCTT AGATATTCCT GCCGAAAGGG TGGAAAAACT GCTCTGCGTT
    GGGTCCCGAA GGTTTGATTC TCTGCCGCAG GAGACACAGC AACTGAAAGA GACGTTTCTG
    AAGTGTCGGA GTGATGAGAC GGCACCGGTC ATTATTTTTG TTTCCAAAAT GTTTGCCGTT
    GATGCAAAAG CTTTACCTCA GAACAAGCAG AGGCCATTAA CTCAGGAAGA AATTGCACTG
    AGAAGGGAAC TTGCACGGCA GAGGCATGCT GAAAAAATGG CAATCAATCA AGAGCAACTG
    CCGGCAGAGC AGGTTCCCTC TAGCAATGAA TGCGAAATGC CTGCAAACAA AGAGAACACA
    AAAGGGGATA AACAGACATC ACACAGAGAT CCTGAGGTCG TTAAGCCTGA GAAACAAGAA
    GCTGAAAACA AGAGTTATTT TATTGCATTT GCTCGAGTCT TTAGTGGAAT TGTTCGTAGA
    GGCCAAAAGA TCTTTGTTTT GGGTCCAAAA TATGATCCTG CAGAAACATT ATTAAAGTTA
    CCACTAAACT GCACTCCTGC ATGTGACCTG CCTGGCATCC CCCACATGGC GTGCTGCTCT
    CTGGACAATC TTTATCTTCT CATGGGCCGT GAACTGGAAA ATTTAGAAGA AGTTCCTGTA
    GGCAATGTTT TAGGTATTGG AGGTTTGGAA GAATTTGTTC TAAAGTCAGC AACACTGTCC
    ACTTCACCAG CTTGTCCACC CTTTATTCCT CTTAATTTTG AAGCAACTCC AATTGTCCGG
    GTAGCTGTTG AACCAAAGCA TCCAAGTGAA ATGCCCCAGC TGGTAAAAGG AATGAAGCTT
    TTAAACCAGG CTGACCCTTG CGTGGAGGTT TTAATACAAG AAACTGGAGA GCATGTCCTG
    ATCACTGCTG GAGAAGTTCA CCTACAGCGC TGCTTGGATG ATTTAAGAGA GAGGTTTGCT
    AAGATTCAGG TCAGTTCGTC TGCTCCTATA ATACCGTTTA GAGAAACTAT CATAAGGCCA
    CCTAAAGTTG ATATGGTGAA TGAAGAGATT GGAAAGCAAC AGAAGATTGC GGTCATACAT
    CAAGTCAAAG AAGAGCAGAG CAAGTGTCCT GAAGGAGTCC AGGTTGACCC TGATGGCTTA
    GTCACCTTGA CTACACCAAA TAAGCTAGCT ACACTGAGTG TCAGGGCCAT GCCCCTTCCA
    GAAGAAGTGA CGCAGCTTCT GGAAAAAAAC AGTGATTTAA TAAGAACAAT GGAGCAAATA
    AATATGGCTT TAAATGAAGG TAGCTACACC ATTCATTTTA ATGAGTCAGC CATTGAAAGG
    ATAACCGCAT TCAAAAGTAA TCTCCAGCAA CTCCTGCAAG GAAGAAGGTG GAGGAATGCT
    GTGGACCAGA TATGGTCCTT TGGCCCAAGA CGTTATGGCC CAAATATCTT ATTGAACCGA
    ATTGAAGGTT ATGACAGGCC ATCAGTTTGG CAGTGCCTTG AAAAAAGTAT TAGAGAGGGG
    AAATATAGAA ATTTTGACAA CAGCATTGTT AGTGGATTTC AGCTAGCAAC TCTTGCTGGC
    CCCATGTGCG AGGAACCACT AATGGGTGTG TGCTTTATTG TGGAAAAGCT GGATCTGAGC
    AGGCCTTTGC TTGCTAAAGA TAGTGCTAGC CAAGATTCAG AAGTGGATAC CAGAACAGAG
    CCAGAACATA GTGAAACTGG GACAACTGGG ACAACTGGGG CCTTAATCAA TAATATCTCA
    ACATCAGCCA CTGAGGAAAC AGAGTTAATT CCTGAAAATG GTGCAAGACC TTTCAGCAAA
    CGAAAAGGTG ACTTATTGAC AGATTGCTAT GGGCCTTTTT CTGGACAACT TATAGCCACT
    ATGAAGGAAG CTTGTCGTTA TGCATTCCAA GTTAAACCCC AGAGACTCAT GGCTGCCATG
    TACACGTGTG AAATAATGGC AACTGCTGAA GTGTTAGGTC GAGTCTATGG TGTCCTGTCA
    AAAAGAGAAG GCCGTGTTCT GTTGGAAGAG ATGAAAGAAG GAACAGACAT GTTCATTATC
    AAGGCAGTGC TGCCGGTGGC TGAAAGCTTT GGCTTTGCTG ATGAAATAAG AAAGAGAACA
    AGTGGCCTGG CCAGCCCACA GCTGGTGTTC AGCCACTGGG AGGTGATTTC TAGTGATCCA
    TTCTGGGTAC CAACCACAGA GGAAGAATAC TTGCACTTTG GAGAAAAGGC AGACTCTGAA
    AACCAGGCCA GAAAATACAT GAACTCTGTG AGGAAGAGGA AAGGACTTTA TGTAGAAGAA
    AAGATTGTGG AACATGCCGA GAAACAGAGA ACTCTTGGCA AGAACAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912678.2
    CDS103..3513
    Translation

    Target ORF information:

    RefSeq Version XM_004912621.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis elongation factor like GTPase 1 (efl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912621.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGACTTG TCAGTTTAGA GAAAATCGTT ACCCTTCAAA AGAGGGCTGC TAACATTCGG 
    AATATATGTA TATTGGCACA TGTGGACCAT GGTAAAACAA CATTGGCTGA CTGCCTCATA
    TCTAGCAATG GTATAATTTC CAACAGACTG GCAGGAAAGT TGCGATATTT GGACAGCCGT
    GAAGATGAAC AAATCCGAGG AATCACAATG AAATCCAGTG CAATTTCTCT GCATTACAAA
    GATGATGAAG AAGAATATCT TATCAATCTC ATTGACTCAC CTGGGCATGT TGACTTTTCT
    TCAGAGGTCT CAACGGCTGT CCGTCTTTGT GATGGATGCA TTATTGTGGT TGATTCTGTA
    GAAGGAGTCT GTCCTCAGAC ACAGGCAGTA TTACGGCAAG CTTGGCTTGA GAATATCCGT
    CCGGTCCTGG TGATCAATAA AATAGATAGG TTGATAACTG AATTGAAACT TTCCTGTTTA
    GAAGCTTATT CCCATCTCCA AAAGATACTG GAGCAGGTCA ACGCAGTTAC TGGAAGTCTA
    TTCACCTCTA AGGTCCTAGA AGAGAGAGCA GAGAAGGACA TGGCAAGTGA TGTTCCATCT
    GAGACTGTGG ATGGAGATCA GGTGTATGAT TGGAGTGCTG GTCTGGAAGA GACAGATGAT
    TCCCACCTTT ATTTTTCACC AGACCAGGGC AATGTTGTAT TTGCCAGTGC CATAGATGGC
    TGGGGATTTA CCATTGATCA CTTTGCCCAA CTGTACAGTC AGAAAGTTGG TATTAAGGCT
    GCTGTACTTC TGAAGACACT CTGGGGAGAT TACTATCTGA ATACCAAGGC CAAGAAGATC
    ATGAAAGGCG CTCAGAGCAA AGGGAAGAAA CCTTTGTTTG TACAACTCAT CTTGGATAAT
    CTTTGGAGTC TGTATGATGC TGTTATTAAA AGGGAGAAGG AAAAGATAGA TAAAATAGTT
    TTATCCTTGG GGTTAAAAAT CAGCCCTCGG GAATCCCGAC ACTCTGACCC AAAAGTACAT
    GTGAATGCTA TCTGCAGCCA GTGGCTTCCA ATATCACAGG CAGTTCTCTC CATGGTATGC
    AGGAAACTGC CAAGCCCCTT AGATATTCCT GCCGAAAGGG TGGAAAAACT GCTCTGCGTT
    GGGTCCCGAA GGTTTGATTC TCTGCCGCAG GAGACACAGC AACTGAAAGA GACGTTTCTG
    AAGTGTCGGA GTGATGAGAC GGCACCGGTC ATTATTTTTG TTTCCAAAAT GTTTGCCGTT
    GATGCAAAAG CTTTACCTCA GAACAAGCAG AGGCCATTAA CTCAGGAAGA AATTGCACTG
    AGAAGGGAAC TTGCACGGCA GAGGCATGCT GAAAAAATGG CAATCAATCA AGAGCAACTG
    CCGGCAGAGC AGGTTCCCTC TAGCAATGAA TGCGAAATGC CTGCAAACAA AGAGAACACA
    AAAGGGGATA AACAGACATC ACACAGAGAT CCTGAGGTCG TTAAGCCTGA GAAACAAGAA
    GCTGAAAACA AGAGTTATTT TATTGCATTT GCTCGAGTCT TTAGTGGAAT TGTTCGTAGA
    GGCCAAAAGA TCTTTGTTTT GGGTCCAAAA TATGATCCTG CAGAAACATT ATTAAAGTTA
    CCACTAAACT GCACTCCTGC ATGTGACCTG CCTGGCATCC CCCACATGGC GTGCTGCTCT
    CTGGACAATC TTTATCTTCT CATGGGCCGT GAACTGGAAA ATTTAGAAGA AGTTCCTGTA
    GGCAATGTTT TAGGTATTGG AGGTTTGGAA GAATTTGTTC TAAAGTCAGC AACACTGTCC
    ACTTCACCAG CTTGTCCACC CTTTATTCCT CTTAATTTTG AAGCAACTCC AATTGTCCGG
    GTAGCTGTTG AACCAAAGCA TCCAAGTGAA ATGCCCCAGC TGGTAAAAGG AATGAAGCTT
    TTAAACCAGG CTGACCCTTG CGTGGAGGTT TTAATACAAG AAACTGGAGA GCATGTCCTG
    ATCACTGCTG GAGAAGTTCA CCTACAGCGC TGCTTGGATG ATTTAAGAGA GAGGTTTGCT
    AAGATTCAGG TCAGTTCGTC TGCTCCTATA ATACCGTTTA GAGAAACTAT CATAAGGCCA
    CCTAAAGTTG ATATGGTGAA TGAAGAGATT GGAAAGCAAC AGAAGATTGC GGTCATACAT
    CAAGTCAAAG AAGAGCAGAG CAAGTGTCCT GAAGGAGTCC AGGTTGACCC TGATGGCTTA
    GTCACCTTGA CTACACCAAA TAAGCTAGCT ACACTGAGTG TCAGGGCCAT GCCCCTTCCA
    GAAGAAGTGA CGCAGCTTCT GGAAAAAAAC AGTGATTTAA TAAGAACAAT GGAGCAAATA
    AATATGGCTT TAAATGAAGG TAGCTACACC ATTCATTTTA ATGAGTCAGC CATTGAAAGG
    ATAACCGCAT TCAAAAGTAA TCTCCAGCAA CTCCTGCAAG GAAGAAGGTG GAGGAATGCT
    GTGGACCAGA TATGGTCCTT TGGCCCAAGA CGTTATGGCC CAAATATCTT ATTGAACCGA
    ATTGAAGGTT ATGACAGGCC ATCAGTTTGG CAGTGCCTTG AAAAAAGTAT TAGAGAGGGG
    AAATATAGAA ATTTTGACAA CAGCATTGTT AGTGGATTTC AGCTAGCAAC TCTTGCTGGC
    CCCATGTGCG AGGAACCACT AATGGGTGTG TGCTTTATTG TGGAAAAGCT GGATCTGAGC
    AGGCCTTTGC TTGCTAAAGA TAGTGCTAGC CAAGATTCAG AAGTGGATAC CAGAACAGAG
    CCAGAACATA GTGAAACTGG GACAACTGGG ACAACTGGGG CCTTAATCAA TAATATCTCA
    ACATCAGCCA CTGAGGAAAC AGAGTTAATT CCTGAAAATG GTGCAAGACC TTTCAGCAAA
    CGAAAAGGTG ACTTATTGAC AGATTGCTAT GGGCCTTTTT CTGGACAACT TATAGCCACT
    ATGAAGGAAG CTTGTCGTTA TGCATTCCAA GTTAAACCCC AGAGACTCAT GGCTGCCATG
    TACACGTGTG AAATAATGGC AACTGCTGAA GTGTTAGGTC GAGTCTATGG TGTCCTGTCA
    AAAAGAGAAG GCCGTGTTCT GTTGGAAGAG ATGAAAGAAG GAACAGACAT GTTCATTATC
    AAGGCAGTGC TGCCGGTGGC TGAAAGCTTT GGCTTTGCTG ATGAAATAAG AAAGAGAACA
    AGTGGCCTGG CCAGCCCACA GCTGGTGTTC AGCCACTGGG AGGTGATTTC TAGTGATCCA
    TTCTGGGTAC CAACCACAGA GGAAGAATAC TTGCACTTTG GAGAAAAGGC AGACTCTGAA
    AACCAGGCCA GAAAATACAT GAACTCTGTG AGGAAGAGGA AAGGACTTTA TGTAGAAGAA
    AAGATTGTGG AACATGCCGA GAAACAGAGA ACTCTTGGCA AGAACAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07240
    Clone ID Related Accession (Same CDS sequence) XM_004912621.3 , XM_012959520.2 , XM_002940100.4
    Accession Version XM_004912621.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product elongation factor-like GTPase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004912621.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGACTTG TCAGTTTAGA GAAAATCGTT ACCCTTCAAA AGAGGGCTGC TAACATTCGG 
    AATATATGTA TATTGGCACA TGTGGACCAT GGTAAAACAA CATTGGCTGA CTGCCTCATA
    TCTAGCAATG GTATAATTTC CAACAGACTG GCAGGAAAGT TGCGATATTT GGACAGCCGT
    GAAGATGAAC AAATCCGAGG AATCACAATG AAATCCAGTG CAATTTCTCT GCATTACAAA
    GATGATGAAG AAGAATATCT TATCAATCTC ATTGACTCAC CTGGGCATGT TGACTTTTCT
    TCAGAGGTCT CAACGGCTGT CCGTCTTTGT GATGGATGCA TTATTGTGGT TGATTCTGTA
    GAAGGAGTCT GTCCTCAGAC ACAGGCAGTA TTACGGCAAG CTTGGCTTGA GAATATCCGT
    CCGGTCCTGG TGATCAATAA AATAGATAGG TTGATAACTG AATTGAAACT TTCCTGTTTA
    GAAGCTTATT CCCATCTCCA AAAGATACTG GAGCAGGTCA ACGCAGTTAC TGGAAGTCTA
    TTCACCTCTA AGGTCCTAGA AGAGAGAGCA GAGAAGGACG TGGCAAGTGA TGTTCCATCT
    GAGACTGTGG ATGGAGATCA GGTGTATGAT TGGAGTGCTG GTCTGGAAGA GACAGATGAT
    TCCCACCTTT ATTTTTCACC AGACCAGGGC AATGTTGTAT TTGCCAGTGC CATAGATGGC
    TGGGGATTTA CCATTGATCA CTTTGCCCAA CTGTACAGTC AGAAAGTTGG TATTAAGGCT
    GCTGTACTTC TGAAGACACT CTGGGGAGAT TACTATCTGA ATACCAAGGC CAAGAAGATC
    ATGAAAGGCG CTCAGAGCAA AGGGAAGAAA CCTTTGTTTG TACAACTCAT CTTGGATAAT
    CTTTGGAGTC TGTATGATGC TGTTATTAAA AGGGAGAAGG AAAAGATAGA TAAAATAGTT
    TTATCCTTGG GGTTAAAAAT CAGCCCTCGG GAATCCCGAC ACTCTGACCC AAAAGTACAT
    GTGAATGCTA TCTGCAGCCA GTGGCTTCCA ATATCACAGG CAGTTCTCTC CATGGTATGC
    AGGAAACTGC CAAGCCCCTT AGATATTCCT GCCGAAAGGG TGGAAAAACT GCTCTGCGTT
    GGGTCCCGAA GGTTTGATTC TCTGCCGCAG GAGACACAGC AACTGAAAGA GACGTTTCTG
    AAGTGTCGGA GTGATGAGAC GGCACCGGTC ATTATTTTTG TTTCCAAAAT GTTTGCCGTT
    GATGCAAAAG CTTTACCTCA GAACAAGCAG AGGCCATTAA CTCAGGAAGA AATTGCACTG
    AGAAGGGAAC TTGCACGACA GAGGCATGCT GAAAAAATGG CAATCAATCA AGAGCAACTG
    CCAGCAGAGC AGGTTCCCTC TAGCAATGAA TGCGAAATGC CTGCAAACAA AGAGAACACA
    AAAGGGGATA AACAGACATC ACACAGAGAT CCTGAGGTCG TTAAGCCTGA GAAACAAGAA
    GCTGAAAACA AGAGTTATTT TATTGCATTT GCTCGAGTCT TTAGTGGAAT TGTTCGTAGA
    GGCCAAAAGA TCTTTGTTTT GGGTCCAAAA TATGATCCTG CAGAAACATT ATTAAAGTTA
    CCACTAAACT GCACTCCTGC ATGTGACCTG CCTGGCATCC CCCACATGGC GTGCTGCTCT
    CTGGACAATC TTTATCTTCT CATGGGCCGT GAACTGGAAA ATTTAGAAGA AGTTCCTGTA
    GGCAATGTTT TAGGTATTGG AGGTTTGGAA GAATTTGTTC TAAAGTCAGC AACACTGTCC
    ACTTCACCAG CTTGTCCACC CTTTATTCCT CTTAATTTTG AAGCAACTCC AATTGTCCGG
    GTAGCTGTTG AACCAAAGCA TCCAAGTGAA ATGCCCCAGC TGGTAAAAGG AATGAAGCTT
    TTAAACCAGG CTGACCCTTG CGTGGAGGTT TTAATACAAG AAACTGGAGA GCATGTCCTG
    ATCACTGCTG GAGAAGTTCA CCTACAGCGC TGCTTGGATG ATTTAAGAGA GAGGTTTGCT
    AAGATTCAGG TCAGTTCGTC TGCTCCTATA ATACCGTTTA GAGAAACTAT CATAAGGCCA
    CCTAAAGTTG ATATGGTGAA TGAAGAGATT GGAAAGCAAC AGAAGATTGC GGTCATACAT
    CAAGTCAAAG AAGAGCAGAG CAAGTGTCCT GAAGGAGTCC AGGTTGACCC TGATGGCTTA
    GTCACCTTGA CTACACCAAA TAAGCTAGCT ACACTGAGTG TCAGGGCCAT GCCCCTTCCA
    GAAGAAGTGA CGCAGCTTCT GGAAAAAAAC AGTGATTTAA TAAGAACAAT GGAGCAAATA
    AATATGGCTT TAAATGAAGG TAGCTACACC ATTCATTTTA ATGAGTCAGC CATTGAAAGG
    ATAACCGCAT TCAAAAGTAA TCTCCAGCAA CTCCTGCAAG GAAGAAGGTG GAGGAATGCT
    GTGGACCAGA TATGGTCCTT TGGCCCAAGA CGTTATGGCC CAAATATCTT ATTGAACCGA
    ATTGAAGGTT ATGACAGGCC ATCAGTTTGG CAGTGCCTTG AAAAAAGTAT TAGAGAGGGG
    AAATATAGAA ATTTTGACAA CAGCATTGTT AGTGGATTTC AGCTAGCAAC TCTTGCTGGC
    CCCATGTGCG AGGAACCACT AATGGGTGTG TGCTTTATTG TGGAAAAGCT GGATCTGAGC
    AGGCCTTTGC TTGCTAAAGA TAGTGCTAGC CAAGATTCAG AAGTGGATAC CAGAACAGAG
    CCAGAACATA GTGAAACTGG GACAACTGGG ACAACTGGGG CCTTAATCAA TAATATCTCA
    ACATCAGCCA CTGAGGAAAC AGAGTTAATT CCTGAAAATG GTGCAAGACC TTTCAGCAAA
    CGAAAAGGTG ACTTATTGAC AGATTGCTAT GGGCCTTTTT CTGGACAACT TATAGCCACT
    ATGAAGGAAG CTTGTCGTTA TGCATTCCAA GTTAAACCCC AGAGACTCAT GGCTGCCATG
    TACACGTGTG AAATAATGGC AACTGCTGAA GTGTTAGGTC GAGTCTATGG TGTCCTGTCA
    AAAAGAGAAG GCCGTGTTCT GTTGGAAGAG ATGAAAGAAG GAACAGACAT GTTCATTATC
    AAGGCAGTGC TGCCGGTGGC TGAAAGCTTT GGCTTTGCTG ATGAAATAAG AAAGAGAACA
    AGTGGCCTGG CCAGCCCACA GCTGGTGTTC AGCCACTGGG AGGTGATTTC TAGTGATCCA
    TTCTGGGTAC CAACCACAGA GGAAGAATAC TTGCACTTTG GAGAAAAGGC AGACTCTGAA
    AACCAGGCCA GAAAATACAT GAACTCTGTG AGGAAGAGGA AAGGACTTTA TGTAGAAGAA
    AAGATTGTGG AACATGCCGA GAAACAGAGA ACTCTTGGCA AGAACAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004912678.1
    CDS94..3504
    Translation

    Target ORF information:

    RefSeq Version XM_004912621.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis elongation factor like GTPase 1 (efl1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004912621.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGACTTG TCAGTTTAGA GAAAATCGTT ACCCTTCAAA AGAGGGCTGC TAACATTCGG 
    AATATATGTA TATTGGCACA TGTGGACCAT GGTAAAACAA CATTGGCTGA CTGCCTCATA
    TCTAGCAATG GTATAATTTC CAACAGACTG GCAGGAAAGT TGCGATATTT GGACAGCCGT
    GAAGATGAAC AAATCCGAGG AATCACAATG AAATCCAGTG CAATTTCTCT GCATTACAAA
    GATGATGAAG AAGAATATCT TATCAATCTC ATTGACTCAC CTGGGCATGT TGACTTTTCT
    TCAGAGGTCT CAACGGCTGT CCGTCTTTGT GATGGATGCA TTATTGTGGT TGATTCTGTA
    GAAGGAGTCT GTCCTCAGAC ACAGGCAGTA TTACGGCAAG CTTGGCTTGA GAATATCCGT
    CCGGTCCTGG TGATCAATAA AATAGATAGG TTGATAACTG AATTGAAACT TTCCTGTTTA
    GAAGCTTATT CCCATCTCCA AAAGATACTG GAGCAGGTCA ACGCAGTTAC TGGAAGTCTA
    TTCACCTCTA AGGTCCTAGA AGAGAGAGCA GAGAAGGACG TGGCAAGTGA TGTTCCATCT
    GAGACTGTGG ATGGAGATCA GGTGTATGAT TGGAGTGCTG GTCTGGAAGA GACAGATGAT
    TCCCACCTTT ATTTTTCACC AGACCAGGGC AATGTTGTAT TTGCCAGTGC CATAGATGGC
    TGGGGATTTA CCATTGATCA CTTTGCCCAA CTGTACAGTC AGAAAGTTGG TATTAAGGCT
    GCTGTACTTC TGAAGACACT CTGGGGAGAT TACTATCTGA ATACCAAGGC CAAGAAGATC
    ATGAAAGGCG CTCAGAGCAA AGGGAAGAAA CCTTTGTTTG TACAACTCAT CTTGGATAAT
    CTTTGGAGTC TGTATGATGC TGTTATTAAA AGGGAGAAGG AAAAGATAGA TAAAATAGTT
    TTATCCTTGG GGTTAAAAAT CAGCCCTCGG GAATCCCGAC ACTCTGACCC AAAAGTACAT
    GTGAATGCTA TCTGCAGCCA GTGGCTTCCA ATATCACAGG CAGTTCTCTC CATGGTATGC
    AGGAAACTGC CAAGCCCCTT AGATATTCCT GCCGAAAGGG TGGAAAAACT GCTCTGCGTT
    GGGTCCCGAA GGTTTGATTC TCTGCCGCAG GAGACACAGC AACTGAAAGA GACGTTTCTG
    AAGTGTCGGA GTGATGAGAC GGCACCGGTC ATTATTTTTG TTTCCAAAAT GTTTGCCGTT
    GATGCAAAAG CTTTACCTCA GAACAAGCAG AGGCCATTAA CTCAGGAAGA AATTGCACTG
    AGAAGGGAAC TTGCACGACA GAGGCATGCT GAAAAAATGG CAATCAATCA AGAGCAACTG
    CCAGCAGAGC AGGTTCCCTC TAGCAATGAA TGCGAAATGC CTGCAAACAA AGAGAACACA
    AAAGGGGATA AACAGACATC ACACAGAGAT CCTGAGGTCG TTAAGCCTGA GAAACAAGAA
    GCTGAAAACA AGAGTTATTT TATTGCATTT GCTCGAGTCT TTAGTGGAAT TGTTCGTAGA
    GGCCAAAAGA TCTTTGTTTT GGGTCCAAAA TATGATCCTG CAGAAACATT ATTAAAGTTA
    CCACTAAACT GCACTCCTGC ATGTGACCTG CCTGGCATCC CCCACATGGC GTGCTGCTCT
    CTGGACAATC TTTATCTTCT CATGGGCCGT GAACTGGAAA ATTTAGAAGA AGTTCCTGTA
    GGCAATGTTT TAGGTATTGG AGGTTTGGAA GAATTTGTTC TAAAGTCAGC AACACTGTCC
    ACTTCACCAG CTTGTCCACC CTTTATTCCT CTTAATTTTG AAGCAACTCC AATTGTCCGG
    GTAGCTGTTG AACCAAAGCA TCCAAGTGAA ATGCCCCAGC TGGTAAAAGG AATGAAGCTT
    TTAAACCAGG CTGACCCTTG CGTGGAGGTT TTAATACAAG AAACTGGAGA GCATGTCCTG
    ATCACTGCTG GAGAAGTTCA CCTACAGCGC TGCTTGGATG ATTTAAGAGA GAGGTTTGCT
    AAGATTCAGG TCAGTTCGTC TGCTCCTATA ATACCGTTTA GAGAAACTAT CATAAGGCCA
    CCTAAAGTTG ATATGGTGAA TGAAGAGATT GGAAAGCAAC AGAAGATTGC GGTCATACAT
    CAAGTCAAAG AAGAGCAGAG CAAGTGTCCT GAAGGAGTCC AGGTTGACCC TGATGGCTTA
    GTCACCTTGA CTACACCAAA TAAGCTAGCT ACACTGAGTG TCAGGGCCAT GCCCCTTCCA
    GAAGAAGTGA CGCAGCTTCT GGAAAAAAAC AGTGATTTAA TAAGAACAAT GGAGCAAATA
    AATATGGCTT TAAATGAAGG TAGCTACACC ATTCATTTTA ATGAGTCAGC CATTGAAAGG
    ATAACCGCAT TCAAAAGTAA TCTCCAGCAA CTCCTGCAAG GAAGAAGGTG GAGGAATGCT
    GTGGACCAGA TATGGTCCTT TGGCCCAAGA CGTTATGGCC CAAATATCTT ATTGAACCGA
    ATTGAAGGTT ATGACAGGCC ATCAGTTTGG CAGTGCCTTG AAAAAAGTAT TAGAGAGGGG
    AAATATAGAA ATTTTGACAA CAGCATTGTT AGTGGATTTC AGCTAGCAAC TCTTGCTGGC
    CCCATGTGCG AGGAACCACT AATGGGTGTG TGCTTTATTG TGGAAAAGCT GGATCTGAGC
    AGGCCTTTGC TTGCTAAAGA TAGTGCTAGC CAAGATTCAG AAGTGGATAC CAGAACAGAG
    CCAGAACATA GTGAAACTGG GACAACTGGG ACAACTGGGG CCTTAATCAA TAATATCTCA
    ACATCAGCCA CTGAGGAAAC AGAGTTAATT CCTGAAAATG GTGCAAGACC TTTCAGCAAA
    CGAAAAGGTG ACTTATTGAC AGATTGCTAT GGGCCTTTTT CTGGACAACT TATAGCCACT
    ATGAAGGAAG CTTGTCGTTA TGCATTCCAA GTTAAACCCC AGAGACTCAT GGCTGCCATG
    TACACGTGTG AAATAATGGC AACTGCTGAA GTGTTAGGTC GAGTCTATGG TGTCCTGTCA
    AAAAGAGAAG GCCGTGTTCT GTTGGAAGAG ATGAAAGAAG GAACAGACAT GTTCATTATC
    AAGGCAGTGC TGCCGGTGGC TGAAAGCTTT GGCTTTGCTG ATGAAATAAG AAAGAGAACA
    AGTGGCCTGG CCAGCCCACA GCTGGTGTTC AGCCACTGGG AGGTGATTTC TAGTGATCCA
    TTCTGGGTAC CAACCACAGA GGAAGAATAC TTGCACTTTG GAGAAAAGGC AGACTCTGAA
    AACCAGGCCA GAAAATACAT GAACTCTGTG AGGAAGAGGA AAGGACTTTA TGTAGAAGAA
    AAGATTGTGG AACATGCCGA GAAACAGAGA ACTCTTGGCA AGAACAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07240
    Clone ID Related Accession (Same CDS sequence) XM_004912621.3 , XM_012959520.2 , XM_002940100.4
    Accession Version XM_012959520.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product elongation factor-like GTPase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGACTTG TCAGTTTAGA GAAAATCGTT ACCCTTCAAA AGAGGGCTGC TAACATTCGG 
    AATATATGTA TATTGGCACA TGTGGACCAT GGTAAAACAA CATTGGCTGA CTGCCTCATA
    TCTAGCAATG GTATAATTTC CAACAGACTG GCAGGAAAGT TGCGATATTT GGACAGCCGT
    GAAGATGAAC AAATCCGAGG AATCACAATG AAATCCAGTG CAATTTCTCT GCATTACAAA
    GATGATGAAG AAGAATATCT TATCAATCTC ATTGACTCAC CTGGGCATGT TGACTTTTCT
    TCAGAGGTCT CAACGGCTGT CCGTCTTTGT GATGGATGCA TTATTGTGGT TGATTCTGTA
    GAAGGAGTCT GTCCTCAGAC ACAGGCAGTA TTACGGCAAG CTTGGCTTGA GAATATCCGT
    CCGGTCCTGG TGATCAATAA AATAGATAGG TTGATAACTG AATTGAAACT TTCCTGTTTA
    GAAGCTTATT CCCATCTCCA AAAGATACTG GAGCAGGTCA ACGCAGTTAC TGGAAGTCTA
    TTCACCTCTA AGGTCCTAGA AGAGAGAGCA GAGAAGGACG TGGCAAGTGA TGTTCCATCT
    GAGACTGTGG ATGGAGATCA GGTGTATGAT TGGAGTGCTG GTCTGGAAGA GACAGATGAT
    TCCCACCTTT ATTTTTCACC AGACCAGGGC AATGTTGTAT TTGCCAGTGC CATAGATGGC
    TGGGGATTTA CCATTGATCA CTTTGCCCAA CTGTACAGTC AGAAAGTTGG TATTAAGGCT
    GCTGTACTTC TGAAGACACT CTGGGGAGAT TACTATCTGA ATACCAAGGC CAAGAAGATC
    ATGAAAGGCG CTCAGAGCAA AGGGAAGAAA CCTTTGTTTG TACAACTCAT CTTGGATAAT
    CTTTGGAGTC TGTATGATGC TGTTATTAAA AGGGAGAAGG AAAAGATAGA TAAAATAGTT
    TTATCCTTGG GGTTAAAAAT CAGCCCTCGG GAATCCCGAC ACTCTGACCC AAAAGTACAT
    GTGAATGCTA TCTGCAGCCA GTGGCTTCCA ATATCACAGG CAGTTCTCTC CATGGTATGC
    AGGAAACTGC CAAGCCCCTT AGATATTCCT GCCGAAAGGG TGGAAAAACT GCTCTGCGTT
    GGGTCCCGAA GGTTTGATTC TCTGCCGCAG GAGACACAGC AACTGAAAGA GACGTTTCTG
    AAGTGTCGGA GTGATGAGAC GGCACCGGTC ATTATTTTTG TTTCCAAAAT GTTTGCCGTT
    GATGCAAAAG CTTTACCTCA GAACAAGCAG AGGCCATTAA CTCAGGAAGA AATTGCACTG
    AGAAGGGAAC TTGCACGACA GAGGCATGCT GAAAAAATGG CAATCAATCA AGAGCAACTG
    CCAGCAGAGC AGGTTCCCTC TAGCAATGAA TGCGAAATGC CTGCAAACAA AGAGAACACA
    AAAGGGGATA AACAGACATC ACACAGAGAT CCTGAGGTCG TTAAGCCTGA GAAACAAGAA
    GCTGAAAACA AGAGTTATTT TATTGCATTT GCTCGAGTCT TTAGTGGAAT TGTTCGTAGA
    GGCCAAAAGA TCTTTGTTTT GGGTCCAAAA TATGATCCTG CAGAAACATT ATTAAAGTTA
    CCACTAAACT GCACTCCTGC ATGTGACCTG CCTGGCATCC CCCACATGGC GTGCTGCTCT
    CTGGACAATC TTTATCTTCT CATGGGCCGT GAACTGGAAA ATTTAGAAGA AGTTCCTGTA
    GGCAATGTTT TAGGTATTGG AGGTTTGGAA GAATTTGTTC TAAAGTCAGC AACACTGTCC
    ACTTCACCAG CTTGTCCACC CTTTATTCCT CTTAATTTTG AAGCAACTCC AATTGTCCGG
    GTAGCTGTTG AACCAAAGCA TCCAAGTGAA ATGCCCCAGC TGGTAAAAGG AATGAAGCTT
    TTAAACCAGG CTGACCCTTG CGTGGAGGTT TTAATACAAG AAACTGGAGA GCATGTCCTG
    ATCACTGCTG GAGAAGTTCA CCTACAGCGC TGCTTGGATG ATTTAAGAGA GAGGTTTGCT
    AAGATTCAGG TCAGTTCGTC TGCTCCTATA ATACCGTTTA GAGAAACTAT CATAAGGCCA
    CCTAAAGTTG ATATGGTGAA TGAAGAGATT GGAAAGCAAC AGAAGATTGC GGTCATACAT
    CAAGTCAAAG AAGAGCAGAG CAAGTGTCCT GAAGGAGTCC AGGTTGACCC TGATGGCTTA
    GTCACCTTGA CTACACCAAA TAAGCTAGCT ACACTGAGTG TCAGGGCCAT GCCCCTTCCA
    GAAGAAGTGA CGCAGCTTCT GGAAAAAAAC AGTGATTTAA TAAGAACAAT GGAGCAAATA
    AATATGGCTT TAAATGAAGG TAGCTACACC ATTCATTTTA ATGAGTCAGC CATTGAAAGG
    ATAACCGCAT TCAAAAGTAA TCTCCAGCAA CTCCTGCAAG GAAGAAGGTG GAGGAATGCT
    GTGGACCAGA TATGGTCCTT TGGCCCAAGA CGTTATGGCC CAAATATCTT ATTGAACCGA
    ATTGAAGGTT ATGACAGGCC ATCAGTTTGG CAGTGCCTTG AAAAAAGTAT TAGAGAGGGG
    AAATATAGAA ATTTTGACAA CAGCATTGTT AGTGGATTTC AGCTAGCAAC TCTTGCTGGC
    CCCATGTGCG AGGAACCACT AATGGGTGTG TGCTTTATTG TGGAAAAGCT GGATCTGAGC
    AGGCCTTTGC TTGCTAAAGA TAGTGCTAGC CAAGATTCAG AAGTGGATAC CAGAACAGAG
    CCAGAACATA GTGAAACTGG GACAACTGGG ACAACTGGGG CCTTAATCAA TAATATCTCA
    ACATCAGCCA CTGAGGAAAC AGAGTTAATT CCTGAAAATG GTGCAAGACC TTTCAGCAAA
    CGAAAAGGTG ACTTATTGAC AGATTGCTAT GGGCCTTTTT CTGGACAACT TATAGCCACT
    ATGAAGGAAG CTTGTCGTTA TGCATTCCAA GTTAAACCCC AGAGACTCAT GGCTGCCATG
    TACACGTGTG AAATAATGGC AACTGCTGAA GTGTTAGGTC GAGTCTATGG TGTCCTGTCA
    AAAAGAGAAG GCCGTGTTCT GTTGGAAGAG ATGAAAGAAG GAACAGACAT GTTCATTATC
    AAGGCAGTGC TGCCGGTGGC TGAAAGCTTT GGCTTTGCTG ATGAAATAAG AAAGAGAACA
    AGTGGCCTGG CCAGCCCACA GCTGGTGTTC AGCCACTGGG AGGTGATTTC TAGTGATCCA
    TTCTGGGTAC CAACCACAGA GGAAGAATAC TTGCACTTTG GAGAAAAGGC AGACTCTGAA
    AACCAGGCCA GAAAATACAT GAACTCTGTG AGGAAGAGGA AAGGACTTTA TGTAGAAGAA
    AAGATTGTGG AACATGCCGA GAAACAGAGA ACTCTTGGCA AGAACAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012814974.1
    CDS28..3438
    Translation

    Target ORF information:

    RefSeq Version XM_012959520.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis elongation factor like GTPase 1 (efl1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959520.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGACTTG TCAGTTTAGA GAAAATCGTT ACCCTTCAAA AGAGGGCTGC TAACATTCGG 
    AATATATGTA TATTGGCACA TGTGGACCAT GGTAAAACAA CATTGGCTGA CTGCCTCATA
    TCTAGCAATG GTATAATTTC CAACAGACTG GCAGGAAAGT TGCGATATTT GGACAGCCGT
    GAAGATGAAC AAATCCGAGG AATCACAATG AAATCCAGTG CAATTTCTCT GCATTACAAA
    GATGATGAAG AAGAATATCT TATCAATCTC ATTGACTCAC CTGGGCATGT TGACTTTTCT
    TCAGAGGTCT CAACGGCTGT CCGTCTTTGT GATGGATGCA TTATTGTGGT TGATTCTGTA
    GAAGGAGTCT GTCCTCAGAC ACAGGCAGTA TTACGGCAAG CTTGGCTTGA GAATATCCGT
    CCGGTCCTGG TGATCAATAA AATAGATAGG TTGATAACTG AATTGAAACT TTCCTGTTTA
    GAAGCTTATT CCCATCTCCA AAAGATACTG GAGCAGGTCA ACGCAGTTAC TGGAAGTCTA
    TTCACCTCTA AGGTCCTAGA AGAGAGAGCA GAGAAGGACG TGGCAAGTGA TGTTCCATCT
    GAGACTGTGG ATGGAGATCA GGTGTATGAT TGGAGTGCTG GTCTGGAAGA GACAGATGAT
    TCCCACCTTT ATTTTTCACC AGACCAGGGC AATGTTGTAT TTGCCAGTGC CATAGATGGC
    TGGGGATTTA CCATTGATCA CTTTGCCCAA CTGTACAGTC AGAAAGTTGG TATTAAGGCT
    GCTGTACTTC TGAAGACACT CTGGGGAGAT TACTATCTGA ATACCAAGGC CAAGAAGATC
    ATGAAAGGCG CTCAGAGCAA AGGGAAGAAA CCTTTGTTTG TACAACTCAT CTTGGATAAT
    CTTTGGAGTC TGTATGATGC TGTTATTAAA AGGGAGAAGG AAAAGATAGA TAAAATAGTT
    TTATCCTTGG GGTTAAAAAT CAGCCCTCGG GAATCCCGAC ACTCTGACCC AAAAGTACAT
    GTGAATGCTA TCTGCAGCCA GTGGCTTCCA ATATCACAGG CAGTTCTCTC CATGGTATGC
    AGGAAACTGC CAAGCCCCTT AGATATTCCT GCCGAAAGGG TGGAAAAACT GCTCTGCGTT
    GGGTCCCGAA GGTTTGATTC TCTGCCGCAG GAGACACAGC AACTGAAAGA GACGTTTCTG
    AAGTGTCGGA GTGATGAGAC GGCACCGGTC ATTATTTTTG TTTCCAAAAT GTTTGCCGTT
    GATGCAAAAG CTTTACCTCA GAACAAGCAG AGGCCATTAA CTCAGGAAGA AATTGCACTG
    AGAAGGGAAC TTGCACGACA GAGGCATGCT GAAAAAATGG CAATCAATCA AGAGCAACTG
    CCAGCAGAGC AGGTTCCCTC TAGCAATGAA TGCGAAATGC CTGCAAACAA AGAGAACACA
    AAAGGGGATA AACAGACATC ACACAGAGAT CCTGAGGTCG TTAAGCCTGA GAAACAAGAA
    GCTGAAAACA AGAGTTATTT TATTGCATTT GCTCGAGTCT TTAGTGGAAT TGTTCGTAGA
    GGCCAAAAGA TCTTTGTTTT GGGTCCAAAA TATGATCCTG CAGAAACATT ATTAAAGTTA
    CCACTAAACT GCACTCCTGC ATGTGACCTG CCTGGCATCC CCCACATGGC GTGCTGCTCT
    CTGGACAATC TTTATCTTCT CATGGGCCGT GAACTGGAAA ATTTAGAAGA AGTTCCTGTA
    GGCAATGTTT TAGGTATTGG AGGTTTGGAA GAATTTGTTC TAAAGTCAGC AACACTGTCC
    ACTTCACCAG CTTGTCCACC CTTTATTCCT CTTAATTTTG AAGCAACTCC AATTGTCCGG
    GTAGCTGTTG AACCAAAGCA TCCAAGTGAA ATGCCCCAGC TGGTAAAAGG AATGAAGCTT
    TTAAACCAGG CTGACCCTTG CGTGGAGGTT TTAATACAAG AAACTGGAGA GCATGTCCTG
    ATCACTGCTG GAGAAGTTCA CCTACAGCGC TGCTTGGATG ATTTAAGAGA GAGGTTTGCT
    AAGATTCAGG TCAGTTCGTC TGCTCCTATA ATACCGTTTA GAGAAACTAT CATAAGGCCA
    CCTAAAGTTG ATATGGTGAA TGAAGAGATT GGAAAGCAAC AGAAGATTGC GGTCATACAT
    CAAGTCAAAG AAGAGCAGAG CAAGTGTCCT GAAGGAGTCC AGGTTGACCC TGATGGCTTA
    GTCACCTTGA CTACACCAAA TAAGCTAGCT ACACTGAGTG TCAGGGCCAT GCCCCTTCCA
    GAAGAAGTGA CGCAGCTTCT GGAAAAAAAC AGTGATTTAA TAAGAACAAT GGAGCAAATA
    AATATGGCTT TAAATGAAGG TAGCTACACC ATTCATTTTA ATGAGTCAGC CATTGAAAGG
    ATAACCGCAT TCAAAAGTAA TCTCCAGCAA CTCCTGCAAG GAAGAAGGTG GAGGAATGCT
    GTGGACCAGA TATGGTCCTT TGGCCCAAGA CGTTATGGCC CAAATATCTT ATTGAACCGA
    ATTGAAGGTT ATGACAGGCC ATCAGTTTGG CAGTGCCTTG AAAAAAGTAT TAGAGAGGGG
    AAATATAGAA ATTTTGACAA CAGCATTGTT AGTGGATTTC AGCTAGCAAC TCTTGCTGGC
    CCCATGTGCG AGGAACCACT AATGGGTGTG TGCTTTATTG TGGAAAAGCT GGATCTGAGC
    AGGCCTTTGC TTGCTAAAGA TAGTGCTAGC CAAGATTCAG AAGTGGATAC CAGAACAGAG
    CCAGAACATA GTGAAACTGG GACAACTGGG ACAACTGGGG CCTTAATCAA TAATATCTCA
    ACATCAGCCA CTGAGGAAAC AGAGTTAATT CCTGAAAATG GTGCAAGACC TTTCAGCAAA
    CGAAAAGGTG ACTTATTGAC AGATTGCTAT GGGCCTTTTT CTGGACAACT TATAGCCACT
    ATGAAGGAAG CTTGTCGTTA TGCATTCCAA GTTAAACCCC AGAGACTCAT GGCTGCCATG
    TACACGTGTG AAATAATGGC AACTGCTGAA GTGTTAGGTC GAGTCTATGG TGTCCTGTCA
    AAAAGAGAAG GCCGTGTTCT GTTGGAAGAG ATGAAAGAAG GAACAGACAT GTTCATTATC
    AAGGCAGTGC TGCCGGTGGC TGAAAGCTTT GGCTTTGCTG ATGAAATAAG AAAGAGAACA
    AGTGGCCTGG CCAGCCCACA GCTGGTGTTC AGCCACTGGG AGGTGATTTC TAGTGATCCA
    TTCTGGGTAC CAACCACAGA GGAAGAATAC TTGCACTTTG GAGAAAAGGC AGACTCTGAA
    AACCAGGCCA GAAAATACAT GAACTCTGTG AGGAAGAGGA AAGGACTTTA TGTAGAAGAA
    AAGATTGTGG AACATGCCGA GAAACAGAGA ACTCTTGGCA AGAACAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa07240
    Clone ID Related Accession (Same CDS sequence) XM_004912621.3 , XM_012959520.2 , XM_002940100.4
    Accession Version XM_002940100.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product elongation factor-like GTPase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940100.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGACTTG TCAGTTTAGA GAAAATCGTT ACCCTTCAAA AGAGGGCTGC TAACATTCGG 
    AATATATGTA TATTGGCACA TGTGGACCAT GGTAAAACAA CATTGGCTGA CTGCCTCATA
    TCTAGCAATG GTATAATTTC CAACAGACTG GCAGGAAAGT TGCGATATTT GGACAGCCGT
    GAAGATGAAC AAATCCGAGG AATCACAATG AAATCCAGTG CAATTTCTCT GCATTACAAA
    GATGATGAAG AAGAATATCT TATCAATCTC ATTGACTCAC CTGGGCATGT TGACTTTTCT
    TCAGAGGTCT CAACGGCTGT CCGTCTTTGT GATGGATGCA TTATTGTGGT TGATTCTGTA
    GAAGGAGTCT GTCCTCAGAC ACAGGCAGTA TTACGGCAAG CTTGGCTTGA GAATATCCGT
    CCGGTCCTGG TGATCAATAA AATAGATAGG TTGATAACTG AATTGAAACT TTCCTGTTTA
    GAAGCTTATT CCCATCTCCA AAAGATACTG GAGCAGGTCA ACGCAGTTAC TGGAAGTCTA
    TTCACCTCTA AGGTCCTAGA AGAGAGAGCA GAGAAGGACG TGGCAAGTGA TGTTCCATCT
    GAGACTGTGG ATGGAGATCA GGTGTATGAT TGGAGTGCTG GTCTGGAAGA GACAGATGAT
    TCCCACCTTT ATTTTTCACC AGACCAGGGC AATGTTGTAT TTGCCAGTGC CATAGATGGC
    TGGGGATTTA CCATTGATCA CTTTGCCCAA CTGTACAGTC AGAAAGTTGG TATTAAGGCT
    GCTGTACTTC TGAAGACACT CTGGGGAGAT TACTATCTGA ATACCAAGGC CAAGAAGATC
    ATGAAAGGCG CTCAGAGCAA AGGGAAGAAA CCTTTGTTTG TACAACTCAT CTTGGATAAT
    CTTTGGAGTC TGTATGATGC TGTTATTAAA AGGGAGAAGG AAAAGATAGA TAAAATAGTT
    TTATCCTTGG GGTTAAAAAT CAGCCCTCGG GAATCCCGAC ACTCTGACCC AAAAGTACAT
    GTGAATGCTA TCTGCAGCCA GTGGCTTCCA ATATCACAGG CAGTTCTCTC CATGGTATGC
    AGGAAACTGC CAAGCCCCTT AGATATTCCT GCCGAAAGGG TGGAAAAACT GCTCTGCGTT
    GGGTCCCGAA GGTTTGATTC TCTGCCGCAG GAGACACAGC AACTGAAAGA GACGTTTCTG
    AAGTGTCGGA GTGATGAGAC GGCACCGGTC ATTATTTTTG TTTCCAAAAT GTTTGCCGTT
    GATGCAAAAG CTTTACCTCA GAACAAGCAG AGGCCATTAA CTCAGGAAGA AATTGCACTG
    AGAAGGGAAC TTGCACGACA GAGGCATGCT GAAAAAATGG CAATCAATCA AGAGCAACTG
    CCAGCAGAGC AGGTTCCCTC TAGCAATGAA TGCGAAATGC CTGCAAACAA AGAGAACACA
    AAAGGGGATA AACAGACATC ACACAGAGAT CCTGAGGTCG TTAAGCCTGA GAAACAAGAA
    GCTGAAAACA AGAGTTATTT TATTGCATTT GCTCGAGTCT TTAGTGGAAT TGTTCGTAGA
    GGCCAAAAGA TCTTTGTTTT GGGTCCAAAA TATGATCCTG CAGAAACATT ATTAAAGTTA
    CCACTAAACT GCACTCCTGC ATGTGACCTG CCTGGCATCC CCCACATGGC GTGCTGCTCT
    CTGGACAATC TTTATCTTCT CATGGGCCGT GAACTGGAAA ATTTAGAAGA AGTTCCTGTA
    GGCAATGTTT TAGGTATTGG AGGTTTGGAA GAATTTGTTC TAAAGTCAGC AACACTGTCC
    ACTTCACCAG CTTGTCCACC CTTTATTCCT CTTAATTTTG AAGCAACTCC AATTGTCCGG
    GTAGCTGTTG AACCAAAGCA TCCAAGTGAA ATGCCCCAGC TGGTAAAAGG AATGAAGCTT
    TTAAACCAGG CTGACCCTTG CGTGGAGGTT TTAATACAAG AAACTGGAGA GCATGTCCTG
    ATCACTGCTG GAGAAGTTCA CCTACAGCGC TGCTTGGATG ATTTAAGAGA GAGGTTTGCT
    AAGATTCAGG TCAGTTCGTC TGCTCCTATA ATACCGTTTA GAGAAACTAT CATAAGGCCA
    CCTAAAGTTG ATATGGTGAA TGAAGAGATT GGAAAGCAAC AGAAGATTGC GGTCATACAT
    CAAGTCAAAG AAGAGCAGAG CAAGTGTCCT GAAGGAGTCC AGGTTGACCC TGATGGCTTA
    GTCACCTTGA CTACACCAAA TAAGCTAGCT ACACTGAGTG TCAGGGCCAT GCCCCTTCCA
    GAAGAAGTGA CGCAGCTTCT GGAAAAAAAC AGTGATTTAA TAAGAACAAT GGAGCAAATA
    AATATGGCTT TAAATGAAGG TAGCTACACC ATTCATTTTA ATGAGTCAGC CATTGAAAGG
    ATAACCGCAT TCAAAAGTAA TCTCCAGCAA CTCCTGCAAG GAAGAAGGTG GAGGAATGCT
    GTGGACCAGA TATGGTCCTT TGGCCCAAGA CGTTATGGCC CAAATATCTT ATTGAACCGA
    ATTGAAGGTT ATGACAGGCC ATCAGTTTGG CAGTGCCTTG AAAAAAGTAT TAGAGAGGGG
    AAATATAGAA ATTTTGACAA CAGCATTGTT AGTGGATTTC AGCTAGCAAC TCTTGCTGGC
    CCCATGTGCG AGGAACCACT AATGGGTGTG TGCTTTATTG TGGAAAAGCT GGATCTGAGC
    AGGCCTTTGC TTGCTAAAGA TAGTGCTAGC CAAGATTCAG AAGTGGATAC CAGAACAGAG
    CCAGAACATA GTGAAACTGG GACAACTGGG ACAACTGGGG CCTTAATCAA TAATATCTCA
    ACATCAGCCA CTGAGGAAAC AGAGTTAATT CCTGAAAATG GTGCAAGACC TTTCAGCAAA
    CGAAAAGGTG ACTTATTGAC AGATTGCTAT GGGCCTTTTT CTGGACAACT TATAGCCACT
    ATGAAGGAAG CTTGTCGTTA TGCATTCCAA GTTAAACCCC AGAGACTCAT GGCTGCCATG
    TACACGTGTG AAATAATGGC AACTGCTGAA GTGTTAGGTC GAGTCTATGG TGTCCTGTCA
    AAAAGAGAAG GCCGTGTTCT GTTGGAAGAG ATGAAAGAAG GAACAGACAT GTTCATTATC
    AAGGCAGTGC TGCCGGTGGC TGAAAGCTTT GGCTTTGCTG ATGAAATAAG AAAGAGAACA
    AGTGGCCTGG CCAGCCCACA GCTGGTGTTC AGCCACTGGG AGGTGATTTC TAGTGATCCA
    TTCTGGGTAC CAACCACAGA GGAAGAATAC TTGCACTTTG GAGAAAAGGC AGACTCTGAA
    AACCAGGCCA GAAAATACAT GAACTCTGTG AGGAAGAGGA AAGGACTTTA TGTAGAAGAA
    AAGATTGTGG AACATGCCGA GAAACAGAGA ACTCTTGGCA AGAACAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002940146.2
    CDS103..3513
    Translation

    Target ORF information:

    RefSeq Version XM_002940100.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis elongation factor like GTPase 1 (efl1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002940100.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGGACTTG TCAGTTTAGA GAAAATCGTT ACCCTTCAAA AGAGGGCTGC TAACATTCGG 
    AATATATGTA TATTGGCACA TGTGGACCAT GGTAAAACAA CATTGGCTGA CTGCCTCATA
    TCTAGCAATG GTATAATTTC CAACAGACTG GCAGGAAAGT TGCGATATTT GGACAGCCGT
    GAAGATGAAC AAATCCGAGG AATCACAATG AAATCCAGTG CAATTTCTCT GCATTACAAA
    GATGATGAAG AAGAATATCT TATCAATCTC ATTGACTCAC CTGGGCATGT TGACTTTTCT
    TCAGAGGTCT CAACGGCTGT CCGTCTTTGT GATGGATGCA TTATTGTGGT TGATTCTGTA
    GAAGGAGTCT GTCCTCAGAC ACAGGCAGTA TTACGGCAAG CTTGGCTTGA GAATATCCGT
    CCGGTCCTGG TGATCAATAA AATAGATAGG TTGATAACTG AATTGAAACT TTCCTGTTTA
    GAAGCTTATT CCCATCTCCA AAAGATACTG GAGCAGGTCA ACGCAGTTAC TGGAAGTCTA
    TTCACCTCTA AGGTCCTAGA AGAGAGAGCA GAGAAGGACG TGGCAAGTGA TGTTCCATCT
    GAGACTGTGG ATGGAGATCA GGTGTATGAT TGGAGTGCTG GTCTGGAAGA GACAGATGAT
    TCCCACCTTT ATTTTTCACC AGACCAGGGC AATGTTGTAT TTGCCAGTGC CATAGATGGC
    TGGGGATTTA CCATTGATCA CTTTGCCCAA CTGTACAGTC AGAAAGTTGG TATTAAGGCT
    GCTGTACTTC TGAAGACACT CTGGGGAGAT TACTATCTGA ATACCAAGGC CAAGAAGATC
    ATGAAAGGCG CTCAGAGCAA AGGGAAGAAA CCTTTGTTTG TACAACTCAT CTTGGATAAT
    CTTTGGAGTC TGTATGATGC TGTTATTAAA AGGGAGAAGG AAAAGATAGA TAAAATAGTT
    TTATCCTTGG GGTTAAAAAT CAGCCCTCGG GAATCCCGAC ACTCTGACCC AAAAGTACAT
    GTGAATGCTA TCTGCAGCCA GTGGCTTCCA ATATCACAGG CAGTTCTCTC CATGGTATGC
    AGGAAACTGC CAAGCCCCTT AGATATTCCT GCCGAAAGGG TGGAAAAACT GCTCTGCGTT
    GGGTCCCGAA GGTTTGATTC TCTGCCGCAG GAGACACAGC AACTGAAAGA GACGTTTCTG
    AAGTGTCGGA GTGATGAGAC GGCACCGGTC ATTATTTTTG TTTCCAAAAT GTTTGCCGTT
    GATGCAAAAG CTTTACCTCA GAACAAGCAG AGGCCATTAA CTCAGGAAGA AATTGCACTG
    AGAAGGGAAC TTGCACGACA GAGGCATGCT GAAAAAATGG CAATCAATCA AGAGCAACTG
    CCAGCAGAGC AGGTTCCCTC TAGCAATGAA TGCGAAATGC CTGCAAACAA AGAGAACACA
    AAAGGGGATA AACAGACATC ACACAGAGAT CCTGAGGTCG TTAAGCCTGA GAAACAAGAA
    GCTGAAAACA AGAGTTATTT TATTGCATTT GCTCGAGTCT TTAGTGGAAT TGTTCGTAGA
    GGCCAAAAGA TCTTTGTTTT GGGTCCAAAA TATGATCCTG CAGAAACATT ATTAAAGTTA
    CCACTAAACT GCACTCCTGC ATGTGACCTG CCTGGCATCC CCCACATGGC GTGCTGCTCT
    CTGGACAATC TTTATCTTCT CATGGGCCGT GAACTGGAAA ATTTAGAAGA AGTTCCTGTA
    GGCAATGTTT TAGGTATTGG AGGTTTGGAA GAATTTGTTC TAAAGTCAGC AACACTGTCC
    ACTTCACCAG CTTGTCCACC CTTTATTCCT CTTAATTTTG AAGCAACTCC AATTGTCCGG
    GTAGCTGTTG AACCAAAGCA TCCAAGTGAA ATGCCCCAGC TGGTAAAAGG AATGAAGCTT
    TTAAACCAGG CTGACCCTTG CGTGGAGGTT TTAATACAAG AAACTGGAGA GCATGTCCTG
    ATCACTGCTG GAGAAGTTCA CCTACAGCGC TGCTTGGATG ATTTAAGAGA GAGGTTTGCT
    AAGATTCAGG TCAGTTCGTC TGCTCCTATA ATACCGTTTA GAGAAACTAT CATAAGGCCA
    CCTAAAGTTG ATATGGTGAA TGAAGAGATT GGAAAGCAAC AGAAGATTGC GGTCATACAT
    CAAGTCAAAG AAGAGCAGAG CAAGTGTCCT GAAGGAGTCC AGGTTGACCC TGATGGCTTA
    GTCACCTTGA CTACACCAAA TAAGCTAGCT ACACTGAGTG TCAGGGCCAT GCCCCTTCCA
    GAAGAAGTGA CGCAGCTTCT GGAAAAAAAC AGTGATTTAA TAAGAACAAT GGAGCAAATA
    AATATGGCTT TAAATGAAGG TAGCTACACC ATTCATTTTA ATGAGTCAGC CATTGAAAGG
    ATAACCGCAT TCAAAAGTAA TCTCCAGCAA CTCCTGCAAG GAAGAAGGTG GAGGAATGCT
    GTGGACCAGA TATGGTCCTT TGGCCCAAGA CGTTATGGCC CAAATATCTT ATTGAACCGA
    ATTGAAGGTT ATGACAGGCC ATCAGTTTGG CAGTGCCTTG AAAAAAGTAT TAGAGAGGGG
    AAATATAGAA ATTTTGACAA CAGCATTGTT AGTGGATTTC AGCTAGCAAC TCTTGCTGGC
    CCCATGTGCG AGGAACCACT AATGGGTGTG TGCTTTATTG TGGAAAAGCT GGATCTGAGC
    AGGCCTTTGC TTGCTAAAGA TAGTGCTAGC CAAGATTCAG AAGTGGATAC CAGAACAGAG
    CCAGAACATA GTGAAACTGG GACAACTGGG ACAACTGGGG CCTTAATCAA TAATATCTCA
    ACATCAGCCA CTGAGGAAAC AGAGTTAATT CCTGAAAATG GTGCAAGACC TTTCAGCAAA
    CGAAAAGGTG ACTTATTGAC AGATTGCTAT GGGCCTTTTT CTGGACAACT TATAGCCACT
    ATGAAGGAAG CTTGTCGTTA TGCATTCCAA GTTAAACCCC AGAGACTCAT GGCTGCCATG
    TACACGTGTG AAATAATGGC AACTGCTGAA GTGTTAGGTC GAGTCTATGG TGTCCTGTCA
    AAAAGAGAAG GCCGTGTTCT GTTGGAAGAG ATGAAAGAAG GAACAGACAT GTTCATTATC
    AAGGCAGTGC TGCCGGTGGC TGAAAGCTTT GGCTTTGCTG ATGAAATAAG AAAGAGAACA
    AGTGGCCTGG CCAGCCCACA GCTGGTGTTC AGCCACTGGG AGGTGATTTC TAGTGATCCA
    TTCTGGGTAC CAACCACAGA GGAAGAATAC TTGCACTTTG GAGAAAAGGC AGACTCTGAA
    AACCAGGCCA GAAAATACAT GAACTCTGTG AGGAAGAGGA AAGGACTTTA TGTAGAAGAA
    AAGATTGTGG AACATGCCGA GAAACAGAGA ACTCTTGGCA AGAACAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.