insr cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following insr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the insr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa04156 XM_002934379.5
Latest version!
Xenopus tropicalis insulin receptor (insr), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa35450 XM_012953588.3
Latest version!
Xenopus tropicalis insulin receptor (insr), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OXa35450 XM_012953588.2 Xenopus tropicalis insulin receptor (insr), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa04156 XM_002934379.4 Xenopus tropicalis insulin receptor (insr), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa04156
    Clone ID Related Accession (Same CDS sequence) XM_002934379.5 , XM_002934379.4
    Accession Version XM_002934379.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product insulin receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934379.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGACAGG GTGTATTGTG GGGTGAGGGG CACCCCAATA ATAACCCCAA GAGCAAGGTG 
    GGCTGGAAGT CCATTGCGGG AATATTCATC ATTTTTATGC TCATTCTGTG CGACCAAAAC
    GACGGGAAAA TTTGCCCCAG TATGGATATC CGGAATAACA TTAGTCAGTT CAGTATGCTT
    GAGGACTGTA CAGTAATTGA AGGTCACCTT CAGATCTTGC TTATGTTCAC CAGCAAACCA
    GAGAACTTCC GAGGGTTGAG ATTTCCCAAG CTCACCATGA TCACAGATTA CCTCCTGCTT
    TTCCGGGTCT ATGGCTTAGA AAGTCTAAAG GACCTTTTTC CCAACCTCAC AGTTATCCGT
    GGTACTCACC TCTTTTTCAA CTATGCCCTA GTCATCTTTG AGATGGTCCA CCTGAAGGAG
    ATTGGGCTCT ACAACCTAAT GAACATTACT AGGGGGTCTG TTAGAATTGA GAAAAATAAT
    GAACTTTGTT ATCTTTCTAC GATTGATTGG TCCATAATTC TTGACTCTGT GGAGGATAAC
    TACATTGAAC TCAACAGGGA CAATAAAGAG GAGTGTGGAG ATGTTTGTCC TGGTGCAGGA
    AAAGGCAAGA GCAAATGCAA ACAGATAGTA GTCAATGGAG CATTCATTGA GCGCTGCTGG
    ACACAGGACC ACTGTCAAAC AGTGTGCCCT TCAGAATGCA AGGGAAGTGG TTGTTTGCCA
    GATGGACAGT GCTGCCACTC AGAGTGCCTT GGAAGCTGCA GAAAGCCTAA TGATCCAACT
    GAGTGTACCG CCTGCCGGCA TTACCAGTAT CAGGGTGTGT GTATCAGTGC ATGTCCCGAG
    GGGTCTTACC GGTTCCAGGG TTGGCGGTGC ATTGACTTCA ATACCTGTCA AGAAATGCAC
    AACCGGTGCC CAAATAAGGA AAATTCCTGT CCTCCTTATG TCATCCACAA GGGAGAGTGC
    ATGCCAGATT GCCCATCAGG CTATATTGCC AACTCAACTA CCCGTACTTG TACACCATGC
    GCAGGACCTT GCCCCAAAGT TTGCACCATC TACCAAAGTG TCAAGACCAT AGACTCTGTG
    ACATCTGCTC AAGAATTAAG AGGGTGCACA GTGGTCAACG GTAGTCTTAT CATCAACATC
    CGTGGAGGCA ATAATATTGC AACAGAATTA GAAGCTAACC TGGGCCTCAT TGAGGAGATT
    TCTGGATATC TTAAGATCAG AAGATCATAT GCATTGGTGT CTTTGTCATT CTTCCGCAAG
    CTGCGTTTAA TTAGGGGAGA AGTGTTGGAA GCAGGTAACT ATTCCTTCTA TGCTCTGGAT
    AATCCCAGTC TACGCCAGCT GTGGGACTGG CATAAGCACA ACCTGACCAT CATACATGGC
    AAGCTCTTCT TCCATCACAA CCCACGACTA TGTCTAGCGG AGATTCACCA AATGGAGGAG
    GTTACAGGAA CAAAGGGCCG GCAAGACAAG AATGATATAT CTACAAAGAC CAATGGAGAT
    CAGGCATCTT GTGAGGACAA TTTACTGAAC TTTAACTTTA TAAGGACATC GCATGATATG
    GTGCTGTTAA AGTGGGATCC TTACTGGCCC CCTGATTACA GAGACCTCTT GGGCTTCATG
    GTGCATTATA AGGAAGCACC CTACCAGAAT GTCACAGAAT TTGATGGTCA GGATGCTTGT
    GGTTCCAATA GTTGGACAGT GGTAGATATG GATGCCCCAG AACGAAGTGC TGATGGCAAG
    ACTCTATCTC CGGGATGTTT GCTCCGTAAC CTGAAACCAT GGACTCAGTA TGCAGTGTTT
    GTTAAAACAC TGGTATCCGG ATCAGATGAA GGAAGAACTT ATGGTGCAAA AAGCAAAATT
    ATCTATATTC GTACCAATGA AACCATCCCC TCTGTTCCTC TGGATCCTTT CTCTGTGTCC
    AACTCAACCT CTCAGATTAT TCTAAAATGG AAACCACCTT CTGAGCCCAA TGGAAATGTC
    ACTCACTACC TTGTGTACTG GCAAGAACAA CCTGAAGACA GTGAACTTTA TGAGCTGGAT
    TACTGCAATA AAGGTCTTAA GTTGCCTTCC AGAACTTGGA CTCCTCCCTC GGATATTGAG
    GAAAATGGAA ATATAAATCA GACAGACCAA CCTTCATCCA AGTGCTGTCC CTGTCCCAAA
    ACTGATTTCC AAATCCAGAA AGAACAGGAT GAATCAGCAT TCAGAAAAAC ATTTGAAAAT
    TATCTACATA ATGAAGTTTT TATACCTAGA CCTGTTCGAA AACGGCGTGA ACTATTTGGA
    GTAGCCAATG GAACTCTGCC AGACCCAGTG ACAGCCCCTT CTCTCTTCAA TGCCTCCTCC
    ACCCGTGCTC CGGATGAAAC AGAGCCAAAG ATATATTCAC AGAAAGTTTG GTTTAAGGAG
    TCTCTAATGG TCACTGGCTT AAAGCATTTT ACTGGTTACC GCATTGAGAT TCATGCCTGC
    AACCACGAAC TCTCCATGGG ATGTAGCGTG GCAGCATATG TTAGTGCAAG GACAATGCCA
    GAGGCCACTG CCGACAATGT TGTGGGACCC ATCACCTATG AGTATATTGA ACCTAATATA
    ATCCATTTAA AATGGCAAGA ACCAAAAGAT CCAAATGGGC TAATAGTTTT GTATGAGGTG
    CATTACTCCC GAGTTGGGGG CACAGAGGAA GTGATTAGCT GCGTGTCACA GAAGCAGTAT
    AGCACAGACA AAGGGGCAAA ACTCAGGGTA TTAACCCCAG GGAATTACAG TGTAAAGATC
    CGTGCAACCT CCCTGGCAGG CAATGGATCA TGGACAGAAC CAGCCTACTT CCAAGTGCCA
    GACCACCCTC ACAGCAGCAT AGTGAAAATC GTCACTGGTC CAGTAATTGC AGTCATTCTT
    CTGCTTTTTG TCCTGGTTTA CTGTGTTATT CAGAAGAAAA AAGATGCAGA AGGCCCAGTT
    GGACCACTCT ACGCCTCCTC TAATCCTGAA TATCTTAGTG CAAGTGAAGT CTACATCCCT
    GACGAATGGG AGGTACCACG TGATAAAATC AGCTTACTGC GAGAATTGGG CCAGGGCTCA
    TTTGGAATGG TGTACGAGGG TATTGCCAAA GATATTATCA AGGGAGAGCC AGAAGTTCGT
    GTTGCTGTGA AAACTGTGAA TGAGTCTGCA AGCCTTCGAG AGAGGATAGA ATTCCTAAAT
    GAGGCTTCTG TCATGAAGGC ATTTAGCTGC CACCATGTGG TCCGTCTGCT TGGTGTGGTG
    TCCAAAGGTC AGCCAACACT TGTCATCATG GAGTTAATGA CGCATGGGGA TCTCAAAAGT
    TACCTGCGCT CACTGCGACC TGATGCAGAG AATAACCCAG GACGTCCTGC TCCTACCCTT
    AAAGAAATGA TCCAGATGGC AGCGGAAATT TCAGATGGCA TGGCATATCT CAATGCTAAA
    AAGTTTGTAC ACAGGGATCT GGCTGCTAGG AACTGCATGG TTGCTGAAGA TTATACTGTG
    AAAATTGGAG ATTTTGGAAT GACACGAGAT ATCTATGAGA CAGATTATTA CCGAAAAGGG
    GGAAAGGGGC TGCTGCCTGT TAGATGGATG TCCCCTGAAT CACTGAAAGA TGGAGTGTTC
    ACAACTTTCT CAGATGTCTG GTCCTTTGGA GTTGTTTTAT GGGAAATAAC GAGTTTGGCA
    GAACAGCCAT ATCAGGGACT TTCTAATGAG CAGGTCCTGA AGTTTGTCAT GGATGGAGGT
    TCACTTGACC AACCGGAGAA TTGCCCTCCA AGGCTGCATA GTCTGATGCA GATGTGCTGG
    CAGTATAACC CCAAGATGCG GCCTACCTTC TTGGAGATTA TCGACATGTT AAAAGATGAC
    CTGCATCTTA GTTTTCAGGA AGTCTCATTC TACTACAGTG ACGAGAACAA GCCACCTGAA
    ACAGATGATT TGGAAATTGA TTTTGAAAAT ATGGAGAGCA CACCCCTGGA CCCTTCTTCC
    TGTTCTTTAC GGGATCAGTC GAATCGGGGG AACATTTATG AAGAACACAT CCCCTACACC
    CATATGAATG GTGGCAGAAA AAATGGACGT ATACTGTCTT TGCCACGGTC CAGCCCATCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934425.2
    CDS1017..5099
    Translation

    Target ORF information:

    RefSeq Version XM_002934379.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis insulin receptor (insr), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934379.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGACAGG GTGTATTGTG GGGTGAGGGG CACCCCAATA ATAACCCCAA GAGCAAGGTG 
    GGCTGGAAGT CCATTGCGGG AATATTCATC ATTTTTATGC TCATTCTGTG CGACCAAAAC
    GACGGGAAAA TTTGCCCCAG TATGGATATC CGGAATAACA TTAGTCAGTT CAGTATGCTT
    GAGGACTGTA CAGTAATTGA AGGTCACCTT CAGATCTTGC TTATGTTCAC CAGCAAACCA
    GAGAACTTCC GAGGGTTGAG ATTTCCCAAG CTCACCATGA TCACAGATTA CCTCCTGCTT
    TTCCGGGTCT ATGGCTTAGA AAGTCTAAAG GACCTTTTTC CCAACCTCAC AGTTATCCGT
    GGTACTCACC TCTTTTTCAA CTATGCCCTA GTCATCTTTG AGATGGTCCA CCTGAAGGAG
    ATTGGGCTCT ACAACCTAAT GAACATTACT AGGGGGTCTG TTAGAATTGA GAAAAATAAT
    GAACTTTGTT ATCTTTCTAC GATTGATTGG TCCATAATTC TTGACTCTGT GGAGGATAAC
    TACATTGAAC TCAACAGGGA CAATAAAGAG GAGTGTGGAG ATGTTTGTCC TGGTGCAGGA
    AAAGGCAAGA GCAAATGCAA ACAGATAGTA GTCAATGGAG CATTCATTGA GCGCTGCTGG
    ACACAGGACC ACTGTCAAAC AGTGTGCCCT TCAGAATGCA AGGGAAGTGG TTGTTTGCCA
    GATGGACAGT GCTGCCACTC AGAGTGCCTT GGAAGCTGCA GAAAGCCTAA TGATCCAACT
    GAGTGTACCG CCTGCCGGCA TTACCAGTAT CAGGGTGTGT GTATCAGTGC ATGTCCCGAG
    GGGTCTTACC GGTTCCAGGG TTGGCGGTGC ATTGACTTCA ATACCTGTCA AGAAATGCAC
    AACCGGTGCC CAAATAAGGA AAATTCCTGT CCTCCTTATG TCATCCACAA GGGAGAGTGC
    ATGCCAGATT GCCCATCAGG CTATATTGCC AACTCAACTA CCCGTACTTG TACACCATGC
    GCAGGACCTT GCCCCAAAGT TTGCACCATC TACCAAAGTG TCAAGACCAT AGACTCTGTG
    ACATCTGCTC AAGAATTAAG AGGGTGCACA GTGGTCAACG GTAGTCTTAT CATCAACATC
    CGTGGAGGCA ATAATATTGC AACAGAATTA GAAGCTAACC TGGGCCTCAT TGAGGAGATT
    TCTGGATATC TTAAGATCAG AAGATCATAT GCATTGGTGT CTTTGTCATT CTTCCGCAAG
    CTGCGTTTAA TTAGGGGAGA AGTGTTGGAA GCAGGTAACT ATTCCTTCTA TGCTCTGGAT
    AATCCCAGTC TACGCCAGCT GTGGGACTGG CATAAGCACA ACCTGACCAT CATACATGGC
    AAGCTCTTCT TCCATCACAA CCCACGACTA TGTCTAGCGG AGATTCACCA AATGGAGGAG
    GTTACAGGAA CAAAGGGCCG GCAAGACAAG AATGATATAT CTACAAAGAC CAATGGAGAT
    CAGGCATCTT GTGAGGACAA TTTACTGAAC TTTAACTTTA TAAGGACATC GCATGATATG
    GTGCTGTTAA AGTGGGATCC TTACTGGCCC CCTGATTACA GAGACCTCTT GGGCTTCATG
    GTGCATTATA AGGAAGCACC CTACCAGAAT GTCACAGAAT TTGATGGTCA GGATGCTTGT
    GGTTCCAATA GTTGGACAGT GGTAGATATG GATGCCCCAG AACGAAGTGC TGATGGCAAG
    ACTCTATCTC CGGGATGTTT GCTCCGTAAC CTGAAACCAT GGACTCAGTA TGCAGTGTTT
    GTTAAAACAC TGGTATCCGG ATCAGATGAA GGAAGAACTT ATGGTGCAAA AAGCAAAATT
    ATCTATATTC GTACCAATGA AACCATCCCC TCTGTTCCTC TGGATCCTTT CTCTGTGTCC
    AACTCAACCT CTCAGATTAT TCTAAAATGG AAACCACCTT CTGAGCCCAA TGGAAATGTC
    ACTCACTACC TTGTGTACTG GCAAGAACAA CCTGAAGACA GTGAACTTTA TGAGCTGGAT
    TACTGCAATA AAGGTCTTAA GTTGCCTTCC AGAACTTGGA CTCCTCCCTC GGATATTGAG
    GAAAATGGAA ATATAAATCA GACAGACCAA CCTTCATCCA AGTGCTGTCC CTGTCCCAAA
    ACTGATTTCC AAATCCAGAA AGAACAGGAT GAATCAGCAT TCAGAAAAAC ATTTGAAAAT
    TATCTACATA ATGAAGTTTT TATACCTAGA CCTGTTCGAA AACGGCGTGA ACTATTTGGA
    GTAGCCAATG GAACTCTGCC AGACCCAGTG ACAGCCCCTT CTCTCTTCAA TGCCTCCTCC
    ACCCGTGCTC CGGATGAAAC AGAGCCAAAG ATATATTCAC AGAAAGTTTG GTTTAAGGAG
    TCTCTAATGG TCACTGGCTT AAAGCATTTT ACTGGTTACC GCATTGAGAT TCATGCCTGC
    AACCACGAAC TCTCCATGGG ATGTAGCGTG GCAGCATATG TTAGTGCAAG GACAATGCCA
    GAGGCCACTG CCGACAATGT TGTGGGACCC ATCACCTATG AGTATATTGA ACCTAATATA
    ATCCATTTAA AATGGCAAGA ACCAAAAGAT CCAAATGGGC TAATAGTTTT GTATGAGGTG
    CATTACTCCC GAGTTGGGGG CACAGAGGAA GTGATTAGCT GCGTGTCACA GAAGCAGTAT
    AGCACAGACA AAGGGGCAAA ACTCAGGGTA TTAACCCCAG GGAATTACAG TGTAAAGATC
    CGTGCAACCT CCCTGGCAGG CAATGGATCA TGGACAGAAC CAGCCTACTT CCAAGTGCCA
    GACCACCCTC ACAGCAGCAT AGTGAAAATC GTCACTGGTC CAGTAATTGC AGTCATTCTT
    CTGCTTTTTG TCCTGGTTTA CTGTGTTATT CAGAAGAAAA AAGATGCAGA AGGCCCAGTT
    GGACCACTCT ACGCCTCCTC TAATCCTGAA TATCTTAGTG CAAGTGAAGT CTACATCCCT
    GACGAATGGG AGGTACCACG TGATAAAATC AGCTTACTGC GAGAATTGGG CCAGGGCTCA
    TTTGGAATGG TGTACGAGGG TATTGCCAAA GATATTATCA AGGGAGAGCC AGAAGTTCGT
    GTTGCTGTGA AAACTGTGAA TGAGTCTGCA AGCCTTCGAG AGAGGATAGA ATTCCTAAAT
    GAGGCTTCTG TCATGAAGGC ATTTAGCTGC CACCATGTGG TCCGTCTGCT TGGTGTGGTG
    TCCAAAGGTC AGCCAACACT TGTCATCATG GAGTTAATGA CGCATGGGGA TCTCAAAAGT
    TACCTGCGCT CACTGCGACC TGATGCAGAG AATAACCCAG GACGTCCTGC TCCTACCCTT
    AAAGAAATGA TCCAGATGGC AGCGGAAATT TCAGATGGCA TGGCATATCT CAATGCTAAA
    AAGTTTGTAC ACAGGGATCT GGCTGCTAGG AACTGCATGG TTGCTGAAGA TTATACTGTG
    AAAATTGGAG ATTTTGGAAT GACACGAGAT ATCTATGAGA CAGATTATTA CCGAAAAGGG
    GGAAAGGGGC TGCTGCCTGT TAGATGGATG TCCCCTGAAT CACTGAAAGA TGGAGTGTTC
    ACAACTTTCT CAGATGTCTG GTCCTTTGGA GTTGTTTTAT GGGAAATAAC GAGTTTGGCA
    GAACAGCCAT ATCAGGGACT TTCTAATGAG CAGGTCCTGA AGTTTGTCAT GGATGGAGGT
    TCACTTGACC AACCGGAGAA TTGCCCTCCA AGGCTGCATA GTCTGATGCA GATGTGCTGG
    CAGTATAACC CCAAGATGCG GCCTACCTTC TTGGAGATTA TCGACATGTT AAAAGATGAC
    CTGCATCTTA GTTTTCAGGA AGTCTCATTC TACTACAGTG ACGAGAACAA GCCACCTGAA
    ACAGATGATT TGGAAATTGA TTTTGAAAAT ATGGAGAGCA CACCCCTGGA CCCTTCTTCC
    TGTTCTTTAC GGGATCAGTC GAATCGGGGG AACATTTATG AAGAACACAT CCCCTACACC
    CATATGAATG GTGGCAGAAA AAATGGACGT ATACTGTCTT TGCCACGGTC CAGCCCATCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35450
    Clone ID Related Accession (Same CDS sequence) XM_012953588.2 , XM_012953588.3
    Accession Version XM_012953588.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product insulin receptor isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012953588.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATATCC GGAATAACAT TAGTCAGTTC AGTATGCTTG AGGACTGTAC AGTAATTGAA 
    GGTCACCTTC AGATCTTGCT TATGTTCACC AGCAAACCAG AGAACTTCCG AGGGTTGAGA
    TTTCCCAAGC TCACCATGAT CACAGATTAC CTCCTGCTTT TCCGGGTCTA TGGCTTAGAA
    AGTCTAAAGG ACCTTTTTCC CAACCTCACA GTTATCCGTG GTACTCACCT CTTTTTCAAC
    TATGCCCTAG TCATCTTTGA GATGGTCCAC CTGAAGGAGA TTGGGCTCTA CAACCTAATG
    AACATTACTA GGGGGTCTGT TAGAATTGAG AAAAATAATG AACTTTGTTA TCTTTCTACG
    ATTGATTGGT CCATAATTCT TGACTCTGTG GAGGATAACT ACATTGAACT CAACAGGGAC
    AATAAAGAGG AGTGTGGAGA TGTTTGTCCT GGTGCAGGAA AAGGCAAGAG CAAATGCAAA
    CAGATAGTAG TCAATGGAGC ATTCATTGAG CGCTGCTGGA CACAGGACCA CTGTCAAACA
    GTGTGCCCTT CAGAATGCAA GGGAAGTGGT TGTTTGCCAG ATGGACAGTG CTGCCACTCA
    GAGTGCCTTG GAAGCTGCAG AAAGCCTAAT GATCCAACTG AGTGTACCGC CTGCCGGCAT
    TACCAGTATC AGGGTGTGTG TATCAGTGCA TGTCCCGAGG GGTCTTACCG GTTCCAGGGT
    TGGCGGTGCA TTGACTTCAA TACCTGTCAA GAAATGCACA ACCGGTGCCC AAATAAGGAA
    AATTCCTGTC CTCCTTATGT CATCCACAAG GGAGAGTGCA TGCCAGATTG CCCATCAGGC
    TATATTGCCA ACTCAACTAC CCGTACTTGT ACACCATGCG CAGGACCTTG CCCCAAAGTT
    TGCACCATCT ACCAAAGTGT CAAGACCATA GACTCTGTGA CATCTGCTCA AGAATTAAGA
    GGGTGCACAG TGGTCAACGG TAGTCTTATC ATCAACATCC GTGGAGGCAA TAATATTGCA
    ACAGAATTAG AAGCTAACCT GGGCCTCATT GAGGAGATTT CTGGATATCT TAAGATCAGA
    AGATCATATG CATTGGTGTC TTTGTCATTC TTCCGCAAGC TGCGTTTAAT TAGGGGAGAA
    GTGTTGGAAG CAGGTAACTA TTCCTTCTAT GCTCTGGATA ATCCCAGTCT ACGCCAGCTG
    TGGGACTGGC ATAAGCACAA CCTGACCATC ATACATGGCA AGCTCTTCTT CCATCACAAC
    CCACGACTAT GTCTAGCGGA GATTCACCAA ATGGAGGAGG TTACAGGAAC AAAGGGCCGG
    CAAGACAAGA ATGATATATC TACAAAGACC AATGGAGATC AGGCATCTTG TGAGGACAAT
    TTACTGAACT TTAACTTTAT AAGGACATCG CATGATATGG TGCTGTTAAA GTGGGATCCT
    TACTGGCCCC CTGATTACAG AGACCTCTTG GGCTTCATGG TGCATTATAA GGAAGCACCC
    TACCAGAATG TCACAGAATT TGATGGTCAG GATGCTTGTG GTTCCAATAG TTGGACAGTG
    GTAGATATGG ATGCCCCAGA ACGAAGTGCT GATGGCAAGA CTCTATCTCC GGGATGTTTG
    CTCCGTAACC TGAAACCATG GACTCAGTAT GCAGTGTTTG TTAAAACACT GGTATCCGGA
    TCAGATGAAG GAAGAACTTA TGGTGCAAAA AGCAAAATTA TCTATATTCG TACCAATGAA
    ACCATCCCCT CTGTTCCTCT GGATCCTTTC TCTGTGTCCA ACTCAACCTC TCAGATTATT
    CTAAAATGGA AACCACCTTC TGAGCCCAAT GGAAATGTCA CTCACTACCT TGTGTACTGG
    CAAGAACAAC CTGAAGACAG TGAACTTTAT GAGCTGGATT ACTGCAATAA AGGTCTTAAG
    TTGCCTTCCA GAACTTGGAC TCCTCCCTCG GATATTGAGG AAAATGGAAA TATAAATCAG
    ACAGACCAAC CTTCATCCAA GTGCTGTCCC TGTCCCAAAA CTGATTTCCA AATCCAGAAA
    GAACAGGATG AATCAGCATT CAGAAAAACA TTTGAAAATT ATCTACATAA TGAAGTTTTT
    ATACCTAGAC CTGTTCGAAA ACGGCGTGAA CTATTTGGAG TAGCCAATGG AACTCTGCCA
    GACCCAGTGA CAGCCCCTTC TCTCTTCAAT GCCTCCTCCA CCCGTGCTCC GGATGAAACA
    GAGCCAAAGA TATATTCACA GAAAGTTTGG TTTAAGGAGT CTCTAATGGT CACTGGCTTA
    AAGCATTTTA CTGGTTACCG CATTGAGATT CATGCCTGCA ACCACGAACT CTCCATGGGA
    TGTAGCGTGG CAGCATATGT TAGTGCAAGG ACAATGCCAG AGGCCACTGC CGACAATGTT
    GTGGGACCCA TCACCTATGA GTATATTGAA CCTAATATAA TCCATTTAAA ATGGCAAGAA
    CCAAAAGATC CAAATGGGCT AATAGTTTTG TATGAGGTGC ATTACTCCCG AGTTGGGGGC
    ACAGAGGAAG TGATTAGCTG CGTGTCACAG AAGCAGTATA GCACAGACAA AGGGGCAAAA
    CTCAGGGTAT TAACCCCAGG GAATTACAGT GTAAAGATCC GTGCAACCTC CCTGGCAGGC
    AATGGATCAT GGACAGAACC AGCCTACTTC CAAGTGCCAG ACCACCCTCA CAGCAGCATA
    GTGAAAATCG TCACTGGTCC AGTAATTGCA GTCATTCTTC TGCTTTTTGT CCTGGTTTAC
    TGTGTTATTC AGAAGAAAAA AGATGCAGAA GGCCCAGTTG GACCACTCTA CGCCTCCTCT
    AATCCTGAAT ATCTTAGTGC AAGTGAAGTC TACATCCCTG ACGAATGGGA GGTACCACGT
    GATAAAATCA GCTTACTGCG AGAATTGGGC CAGGGCTCAT TTGGAATGGT GTACGAGGGT
    ATTGCCAAAG ATATTATCAA GGGAGAGCCA GAAGTTCGTG TTGCTGTGAA AACTGTGAAT
    GAGTCTGCAA GCCTTCGAGA GAGGATAGAA TTCCTAAATG AGGCTTCTGT CATGAAGGCA
    TTTAGCTGCC ACCATGTGGT CCGTCTGCTT GGTGTGGTGT CCAAAGGTCA GCCAACACTT
    GTCATCATGG AGTTAATGAC GCATGGGGAT CTCAAAAGTT ACCTGCGCTC ACTGCGACCT
    GATGCAGAGA ATAACCCAGG ACGTCCTGCT CCTACCCTTA AAGAAATGAT CCAGATGGCA
    GCGGAAATTT CAGATGGCAT GGCATATCTC AATGCTAAAA AGTTTGTACA CAGGGATCTG
    GCTGCTAGGA ACTGCATGGT TGCTGAAGAT TATACTGTGA AAATTGGAGA TTTTGGAATG
    ACACGAGATA TCTATGAGAC AGATTATTAC CGAAAAGGGG GAAAGGGGCT GCTGCCTGTT
    AGATGGATGT CCCCTGAATC ACTGAAAGAT GGAGTGTTCA CAACTTTCTC AGATGTCTGG
    TCCTTTGGAG TTGTTTTATG GGAAATAACG AGTTTGGCAG AACAGCCATA TCAGGGACTT
    TCTAATGAGC AGGTCCTGAA GTTTGTCATG GATGGAGGTT CACTTGACCA ACCGGAGAAT
    TGCCCTCCAA GGCTGCATAG TCTGATGCAG ATGTGCTGGC AGTATAACCC CAAGATGCGG
    CCTACCTTCT TGGAGATTAT CGACATGTTA AAAGATGACC TGCATCTTAG TTTTCAGGAA
    GTCTCATTCT ACTACAGTGA CGAGAACAAG CCACCTGAAA CAGATGATTT GGAAATTGAT
    TTTGAAAATA TGGAGAGCAC ACCCCTGGAC CCTTCTTCCT GTTCTTTACG GGATCAGTCG
    AATCGGGGGA ACATTTATGA AGAACACATC CCCTACACCC ATATGAATGG TGGCAGAAAA
    AATGGACGTA TACTGTCTTT GCCACGGTCC AGCCCATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012809042.1
    CDS1517..5458
    Translation

    Target ORF information:

    RefSeq Version XM_012953588.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis insulin receptor (insr), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012953588.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATATCC GGAATAACAT TAGTCAGTTC AGTATGCTTG AGGACTGTAC AGTAATTGAA 
    GGTCACCTTC AGATCTTGCT TATGTTCACC AGCAAACCAG AGAACTTCCG AGGGTTGAGA
    TTTCCCAAGC TCACCATGAT CACAGATTAC CTCCTGCTTT TCCGGGTCTA TGGCTTAGAA
    AGTCTAAAGG ACCTTTTTCC CAACCTCACA GTTATCCGTG GTACTCACCT CTTTTTCAAC
    TATGCCCTAG TCATCTTTGA GATGGTCCAC CTGAAGGAGA TTGGGCTCTA CAACCTAATG
    AACATTACTA GGGGGTCTGT TAGAATTGAG AAAAATAATG AACTTTGTTA TCTTTCTACG
    ATTGATTGGT CCATAATTCT TGACTCTGTG GAGGATAACT ACATTGAACT CAACAGGGAC
    AATAAAGAGG AGTGTGGAGA TGTTTGTCCT GGTGCAGGAA AAGGCAAGAG CAAATGCAAA
    CAGATAGTAG TCAATGGAGC ATTCATTGAG CGCTGCTGGA CACAGGACCA CTGTCAAACA
    GTGTGCCCTT CAGAATGCAA GGGAAGTGGT TGTTTGCCAG ATGGACAGTG CTGCCACTCA
    GAGTGCCTTG GAAGCTGCAG AAAGCCTAAT GATCCAACTG AGTGTACCGC CTGCCGGCAT
    TACCAGTATC AGGGTGTGTG TATCAGTGCA TGTCCCGAGG GGTCTTACCG GTTCCAGGGT
    TGGCGGTGCA TTGACTTCAA TACCTGTCAA GAAATGCACA ACCGGTGCCC AAATAAGGAA
    AATTCCTGTC CTCCTTATGT CATCCACAAG GGAGAGTGCA TGCCAGATTG CCCATCAGGC
    TATATTGCCA ACTCAACTAC CCGTACTTGT ACACCATGCG CAGGACCTTG CCCCAAAGTT
    TGCACCATCT ACCAAAGTGT CAAGACCATA GACTCTGTGA CATCTGCTCA AGAATTAAGA
    GGGTGCACAG TGGTCAACGG TAGTCTTATC ATCAACATCC GTGGAGGCAA TAATATTGCA
    ACAGAATTAG AAGCTAACCT GGGCCTCATT GAGGAGATTT CTGGATATCT TAAGATCAGA
    AGATCATATG CATTGGTGTC TTTGTCATTC TTCCGCAAGC TGCGTTTAAT TAGGGGAGAA
    GTGTTGGAAG CAGGTAACTA TTCCTTCTAT GCTCTGGATA ATCCCAGTCT ACGCCAGCTG
    TGGGACTGGC ATAAGCACAA CCTGACCATC ATACATGGCA AGCTCTTCTT CCATCACAAC
    CCACGACTAT GTCTAGCGGA GATTCACCAA ATGGAGGAGG TTACAGGAAC AAAGGGCCGG
    CAAGACAAGA ATGATATATC TACAAAGACC AATGGAGATC AGGCATCTTG TGAGGACAAT
    TTACTGAACT TTAACTTTAT AAGGACATCG CATGATATGG TGCTGTTAAA GTGGGATCCT
    TACTGGCCCC CTGATTACAG AGACCTCTTG GGCTTCATGG TGCATTATAA GGAAGCACCC
    TACCAGAATG TCACAGAATT TGATGGTCAG GATGCTTGTG GTTCCAATAG TTGGACAGTG
    GTAGATATGG ATGCCCCAGA ACGAAGTGCT GATGGCAAGA CTCTATCTCC GGGATGTTTG
    CTCCGTAACC TGAAACCATG GACTCAGTAT GCAGTGTTTG TTAAAACACT GGTATCCGGA
    TCAGATGAAG GAAGAACTTA TGGTGCAAAA AGCAAAATTA TCTATATTCG TACCAATGAA
    ACCATCCCCT CTGTTCCTCT GGATCCTTTC TCTGTGTCCA ACTCAACCTC TCAGATTATT
    CTAAAATGGA AACCACCTTC TGAGCCCAAT GGAAATGTCA CTCACTACCT TGTGTACTGG
    CAAGAACAAC CTGAAGACAG TGAACTTTAT GAGCTGGATT ACTGCAATAA AGGTCTTAAG
    TTGCCTTCCA GAACTTGGAC TCCTCCCTCG GATATTGAGG AAAATGGAAA TATAAATCAG
    ACAGACCAAC CTTCATCCAA GTGCTGTCCC TGTCCCAAAA CTGATTTCCA AATCCAGAAA
    GAACAGGATG AATCAGCATT CAGAAAAACA TTTGAAAATT ATCTACATAA TGAAGTTTTT
    ATACCTAGAC CTGTTCGAAA ACGGCGTGAA CTATTTGGAG TAGCCAATGG AACTCTGCCA
    GACCCAGTGA CAGCCCCTTC TCTCTTCAAT GCCTCCTCCA CCCGTGCTCC GGATGAAACA
    GAGCCAAAGA TATATTCACA GAAAGTTTGG TTTAAGGAGT CTCTAATGGT CACTGGCTTA
    AAGCATTTTA CTGGTTACCG CATTGAGATT CATGCCTGCA ACCACGAACT CTCCATGGGA
    TGTAGCGTGG CAGCATATGT TAGTGCAAGG ACAATGCCAG AGGCCACTGC CGACAATGTT
    GTGGGACCCA TCACCTATGA GTATATTGAA CCTAATATAA TCCATTTAAA ATGGCAAGAA
    CCAAAAGATC CAAATGGGCT AATAGTTTTG TATGAGGTGC ATTACTCCCG AGTTGGGGGC
    ACAGAGGAAG TGATTAGCTG CGTGTCACAG AAGCAGTATA GCACAGACAA AGGGGCAAAA
    CTCAGGGTAT TAACCCCAGG GAATTACAGT GTAAAGATCC GTGCAACCTC CCTGGCAGGC
    AATGGATCAT GGACAGAACC AGCCTACTTC CAAGTGCCAG ACCACCCTCA CAGCAGCATA
    GTGAAAATCG TCACTGGTCC AGTAATTGCA GTCATTCTTC TGCTTTTTGT CCTGGTTTAC
    TGTGTTATTC AGAAGAAAAA AGATGCAGAA GGCCCAGTTG GACCACTCTA CGCCTCCTCT
    AATCCTGAAT ATCTTAGTGC AAGTGAAGTC TACATCCCTG ACGAATGGGA GGTACCACGT
    GATAAAATCA GCTTACTGCG AGAATTGGGC CAGGGCTCAT TTGGAATGGT GTACGAGGGT
    ATTGCCAAAG ATATTATCAA GGGAGAGCCA GAAGTTCGTG TTGCTGTGAA AACTGTGAAT
    GAGTCTGCAA GCCTTCGAGA GAGGATAGAA TTCCTAAATG AGGCTTCTGT CATGAAGGCA
    TTTAGCTGCC ACCATGTGGT CCGTCTGCTT GGTGTGGTGT CCAAAGGTCA GCCAACACTT
    GTCATCATGG AGTTAATGAC GCATGGGGAT CTCAAAAGTT ACCTGCGCTC ACTGCGACCT
    GATGCAGAGA ATAACCCAGG ACGTCCTGCT CCTACCCTTA AAGAAATGAT CCAGATGGCA
    GCGGAAATTT CAGATGGCAT GGCATATCTC AATGCTAAAA AGTTTGTACA CAGGGATCTG
    GCTGCTAGGA ACTGCATGGT TGCTGAAGAT TATACTGTGA AAATTGGAGA TTTTGGAATG
    ACACGAGATA TCTATGAGAC AGATTATTAC CGAAAAGGGG GAAAGGGGCT GCTGCCTGTT
    AGATGGATGT CCCCTGAATC ACTGAAAGAT GGAGTGTTCA CAACTTTCTC AGATGTCTGG
    TCCTTTGGAG TTGTTTTATG GGAAATAACG AGTTTGGCAG AACAGCCATA TCAGGGACTT
    TCTAATGAGC AGGTCCTGAA GTTTGTCATG GATGGAGGTT CACTTGACCA ACCGGAGAAT
    TGCCCTCCAA GGCTGCATAG TCTGATGCAG ATGTGCTGGC AGTATAACCC CAAGATGCGG
    CCTACCTTCT TGGAGATTAT CGACATGTTA AAAGATGACC TGCATCTTAG TTTTCAGGAA
    GTCTCATTCT ACTACAGTGA CGAGAACAAG CCACCTGAAA CAGATGATTT GGAAATTGAT
    TTTGAAAATA TGGAGAGCAC ACCCCTGGAC CCTTCTTCCT GTTCTTTACG GGATCAGTCG
    AATCGGGGGA ACATTTATGA AGAACACATC CCCTACACCC ATATGAATGG TGGCAGAAAA
    AATGGACGTA TACTGTCTTT GCCACGGTCC AGCCCATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35450
    Clone ID Related Accession (Same CDS sequence) XM_012953588.2 , XM_012953588.3
    Accession Version XM_012953588.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product insulin receptor isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATATCC GGAATAACAT TAGTCAGTTC AGTATGCTTG AGGACTGTAC AGTAATTGAA 
    GGTCACCTTC AGATCTTGCT TATGTTCACC AGCAAACCAG AGAACTTCCG AGGGTTGAGA
    TTTCCCAAGC TCACCATGAT CACAGATTAC CTCCTGCTTT TCCGGGTCTA TGGCTTAGAA
    AGTCTAAAGG ACCTTTTTCC CAACCTCACA GTTATCCGTG GTACTCACCT CTTTTTCAAC
    TATGCCCTAG TCATCTTTGA GATGGTCCAC CTGAAGGAGA TTGGGCTCTA CAACCTAATG
    AACATTACTA GGGGGTCTGT TAGAATTGAG AAAAATAATG AACTTTGTTA TCTTTCTACG
    ATTGATTGGT CCATAATTCT TGACTCTGTG GAGGATAACT ACATTGAACT CAACAGGGAC
    AATAAAGAGG AGTGTGGAGA TGTTTGTCCT GGTGCAGGAA AAGGCAAGAG CAAATGCAAA
    CAGATAGTAG TCAATGGAGC ATTCATTGAG CGCTGCTGGA CACAGGACCA CTGTCAAACA
    GTGTGCCCTT CAGAATGCAA GGGAAGTGGT TGTTTGCCAG ATGGACAGTG CTGCCACTCA
    GAGTGCCTTG GAAGCTGCAG AAAGCCTAAT GATCCAACTG AGTGTACCGC CTGCCGGCAT
    TACCAGTATC AGGGTGTGTG TATCAGTGCA TGTCCCGAGG GGTCTTACCG GTTCCAGGGT
    TGGCGGTGCA TTGACTTCAA TACCTGTCAA GAAATGCACA ACCGGTGCCC AAATAAGGAA
    AATTCCTGTC CTCCTTATGT CATCCACAAG GGAGAGTGCA TGCCAGATTG CCCATCAGGC
    TATATTGCCA ACTCAACTAC CCGTACTTGT ACACCATGCG CAGGACCTTG CCCCAAAGTT
    TGCACCATCT ACCAAAGTGT CAAGACCATA GACTCTGTGA CATCTGCTCA AGAATTAAGA
    GGGTGCACAG TGGTCAACGG TAGTCTTATC ATCAACATCC GTGGAGGCAA TAATATTGCA
    ACAGAATTAG AAGCTAACCT GGGCCTCATT GAGGAGATTT CTGGATATCT TAAGATCAGA
    AGATCATATG CATTGGTGTC TTTGTCATTC TTCCGCAAGC TGCGTTTAAT TAGGGGAGAA
    GTGTTGGAAG CAGGTAACTA TTCCTTCTAT GCTCTGGATA ATCCCAGTCT ACGCCAGCTG
    TGGGACTGGC ATAAGCACAA CCTGACCATC ATACATGGCA AGCTCTTCTT CCATCACAAC
    CCACGACTAT GTCTAGCGGA GATTCACCAA ATGGAGGAGG TTACAGGAAC AAAGGGCCGG
    CAAGACAAGA ATGATATATC TACAAAGACC AATGGAGATC AGGCATCTTG TGAGGACAAT
    TTACTGAACT TTAACTTTAT AAGGACATCG CATGATATGG TGCTGTTAAA GTGGGATCCT
    TACTGGCCCC CTGATTACAG AGACCTCTTG GGCTTCATGG TGCATTATAA GGAAGCACCC
    TACCAGAATG TCACAGAATT TGATGGTCAG GATGCTTGTG GTTCCAATAG TTGGACAGTG
    GTAGATATGG ATGCCCCAGA ACGAAGTGCT GATGGCAAGA CTCTATCTCC GGGATGTTTG
    CTCCGTAACC TGAAACCATG GACTCAGTAT GCAGTGTTTG TTAAAACACT GGTATCCGGA
    TCAGATGAAG GAAGAACTTA TGGTGCAAAA AGCAAAATTA TCTATATTCG TACCAATGAA
    ACCATCCCCT CTGTTCCTCT GGATCCTTTC TCTGTGTCCA ACTCAACCTC TCAGATTATT
    CTAAAATGGA AACCACCTTC TGAGCCCAAT GGAAATGTCA CTCACTACCT TGTGTACTGG
    CAAGAACAAC CTGAAGACAG TGAACTTTAT GAGCTGGATT ACTGCAATAA AGGTCTTAAG
    TTGCCTTCCA GAACTTGGAC TCCTCCCTCG GATATTGAGG AAAATGGAAA TATAAATCAG
    ACAGACCAAC CTTCATCCAA GTGCTGTCCC TGTCCCAAAA CTGATTTCCA AATCCAGAAA
    GAACAGGATG AATCAGCATT CAGAAAAACA TTTGAAAATT ATCTACATAA TGAAGTTTTT
    ATACCTAGAC CTGTTCGAAA ACGGCGTGAA CTATTTGGAG TAGCCAATGG AACTCTGCCA
    GACCCAGTGA CAGCCCCTTC TCTCTTCAAT GCCTCCTCCA CCCGTGCTCC GGATGAAACA
    GAGCCAAAGA TATATTCACA GAAAGTTTGG TTTAAGGAGT CTCTAATGGT CACTGGCTTA
    AAGCATTTTA CTGGTTACCG CATTGAGATT CATGCCTGCA ACCACGAACT CTCCATGGGA
    TGTAGCGTGG CAGCATATGT TAGTGCAAGG ACAATGCCAG AGGCCACTGC CGACAATGTT
    GTGGGACCCA TCACCTATGA GTATATTGAA CCTAATATAA TCCATTTAAA ATGGCAAGAA
    CCAAAAGATC CAAATGGGCT AATAGTTTTG TATGAGGTGC ATTACTCCCG AGTTGGGGGC
    ACAGAGGAAG TGATTAGCTG CGTGTCACAG AAGCAGTATA GCACAGACAA AGGGGCAAAA
    CTCAGGGTAT TAACCCCAGG GAATTACAGT GTAAAGATCC GTGCAACCTC CCTGGCAGGC
    AATGGATCAT GGACAGAACC AGCCTACTTC CAAGTGCCAG ACCACCCTCA CAGCAGCATA
    GTGAAAATCG TCACTGGTCC AGTAATTGCA GTCATTCTTC TGCTTTTTGT CCTGGTTTAC
    TGTGTTATTC AGAAGAAAAA AGATGCAGAA GGCCCAGTTG GACCACTCTA CGCCTCCTCT
    AATCCTGAAT ATCTTAGTGC AAGTGAAGTC TACATCCCTG ACGAATGGGA GGTACCACGT
    GATAAAATCA GCTTACTGCG AGAATTGGGC CAGGGCTCAT TTGGAATGGT GTACGAGGGT
    ATTGCCAAAG ATATTATCAA GGGAGAGCCA GAAGTTCGTG TTGCTGTGAA AACTGTGAAT
    GAGTCTGCAA GCCTTCGAGA GAGGATAGAA TTCCTAAATG AGGCTTCTGT CATGAAGGCA
    TTTAGCTGCC ACCATGTGGT CCGTCTGCTT GGTGTGGTGT CCAAAGGTCA GCCAACACTT
    GTCATCATGG AGTTAATGAC GCATGGGGAT CTCAAAAGTT ACCTGCGCTC ACTGCGACCT
    GATGCAGAGA ATAACCCAGG ACGTCCTGCT CCTACCCTTA AAGAAATGAT CCAGATGGCA
    GCGGAAATTT CAGATGGCAT GGCATATCTC AATGCTAAAA AGTTTGTACA CAGGGATCTG
    GCTGCTAGGA ACTGCATGGT TGCTGAAGAT TATACTGTGA AAATTGGAGA TTTTGGAATG
    ACACGAGATA TCTATGAGAC AGATTATTAC CGAAAAGGGG GAAAGGGGCT GCTGCCTGTT
    AGATGGATGT CCCCTGAATC ACTGAAAGAT GGAGTGTTCA CAACTTTCTC AGATGTCTGG
    TCCTTTGGAG TTGTTTTATG GGAAATAACG AGTTTGGCAG AACAGCCATA TCAGGGACTT
    TCTAATGAGC AGGTCCTGAA GTTTGTCATG GATGGAGGTT CACTTGACCA ACCGGAGAAT
    TGCCCTCCAA GGCTGCATAG TCTGATGCAG ATGTGCTGGC AGTATAACCC CAAGATGCGG
    CCTACCTTCT TGGAGATTAT CGACATGTTA AAAGATGACC TGCATCTTAG TTTTCAGGAA
    GTCTCATTCT ACTACAGTGA CGAGAACAAG CCACCTGAAA CAGATGATTT GGAAATTGAT
    TTTGAAAATA TGGAGAGCAC ACCCCTGGAC CCTTCTTCCT GTTCTTTACG GGATCAGTCG
    AATCGGGGGA ACATTTATGA AGAACACATC CCCTACACCC ATATGAATGG TGGCAGAAAA
    AATGGACGTA TACTGTCTTT GCCACGGTCC AGCCCATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012809042.1
    CDS1805..5746
    Translation

    Target ORF information:

    RefSeq Version XM_012953588.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis insulin receptor (insr), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012953588.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGATATCC GGAATAACAT TAGTCAGTTC AGTATGCTTG AGGACTGTAC AGTAATTGAA 
    GGTCACCTTC AGATCTTGCT TATGTTCACC AGCAAACCAG AGAACTTCCG AGGGTTGAGA
    TTTCCCAAGC TCACCATGAT CACAGATTAC CTCCTGCTTT TCCGGGTCTA TGGCTTAGAA
    AGTCTAAAGG ACCTTTTTCC CAACCTCACA GTTATCCGTG GTACTCACCT CTTTTTCAAC
    TATGCCCTAG TCATCTTTGA GATGGTCCAC CTGAAGGAGA TTGGGCTCTA CAACCTAATG
    AACATTACTA GGGGGTCTGT TAGAATTGAG AAAAATAATG AACTTTGTTA TCTTTCTACG
    ATTGATTGGT CCATAATTCT TGACTCTGTG GAGGATAACT ACATTGAACT CAACAGGGAC
    AATAAAGAGG AGTGTGGAGA TGTTTGTCCT GGTGCAGGAA AAGGCAAGAG CAAATGCAAA
    CAGATAGTAG TCAATGGAGC ATTCATTGAG CGCTGCTGGA CACAGGACCA CTGTCAAACA
    GTGTGCCCTT CAGAATGCAA GGGAAGTGGT TGTTTGCCAG ATGGACAGTG CTGCCACTCA
    GAGTGCCTTG GAAGCTGCAG AAAGCCTAAT GATCCAACTG AGTGTACCGC CTGCCGGCAT
    TACCAGTATC AGGGTGTGTG TATCAGTGCA TGTCCCGAGG GGTCTTACCG GTTCCAGGGT
    TGGCGGTGCA TTGACTTCAA TACCTGTCAA GAAATGCACA ACCGGTGCCC AAATAAGGAA
    AATTCCTGTC CTCCTTATGT CATCCACAAG GGAGAGTGCA TGCCAGATTG CCCATCAGGC
    TATATTGCCA ACTCAACTAC CCGTACTTGT ACACCATGCG CAGGACCTTG CCCCAAAGTT
    TGCACCATCT ACCAAAGTGT CAAGACCATA GACTCTGTGA CATCTGCTCA AGAATTAAGA
    GGGTGCACAG TGGTCAACGG TAGTCTTATC ATCAACATCC GTGGAGGCAA TAATATTGCA
    ACAGAATTAG AAGCTAACCT GGGCCTCATT GAGGAGATTT CTGGATATCT TAAGATCAGA
    AGATCATATG CATTGGTGTC TTTGTCATTC TTCCGCAAGC TGCGTTTAAT TAGGGGAGAA
    GTGTTGGAAG CAGGTAACTA TTCCTTCTAT GCTCTGGATA ATCCCAGTCT ACGCCAGCTG
    TGGGACTGGC ATAAGCACAA CCTGACCATC ATACATGGCA AGCTCTTCTT CCATCACAAC
    CCACGACTAT GTCTAGCGGA GATTCACCAA ATGGAGGAGG TTACAGGAAC AAAGGGCCGG
    CAAGACAAGA ATGATATATC TACAAAGACC AATGGAGATC AGGCATCTTG TGAGGACAAT
    TTACTGAACT TTAACTTTAT AAGGACATCG CATGATATGG TGCTGTTAAA GTGGGATCCT
    TACTGGCCCC CTGATTACAG AGACCTCTTG GGCTTCATGG TGCATTATAA GGAAGCACCC
    TACCAGAATG TCACAGAATT TGATGGTCAG GATGCTTGTG GTTCCAATAG TTGGACAGTG
    GTAGATATGG ATGCCCCAGA ACGAAGTGCT GATGGCAAGA CTCTATCTCC GGGATGTTTG
    CTCCGTAACC TGAAACCATG GACTCAGTAT GCAGTGTTTG TTAAAACACT GGTATCCGGA
    TCAGATGAAG GAAGAACTTA TGGTGCAAAA AGCAAAATTA TCTATATTCG TACCAATGAA
    ACCATCCCCT CTGTTCCTCT GGATCCTTTC TCTGTGTCCA ACTCAACCTC TCAGATTATT
    CTAAAATGGA AACCACCTTC TGAGCCCAAT GGAAATGTCA CTCACTACCT TGTGTACTGG
    CAAGAACAAC CTGAAGACAG TGAACTTTAT GAGCTGGATT ACTGCAATAA AGGTCTTAAG
    TTGCCTTCCA GAACTTGGAC TCCTCCCTCG GATATTGAGG AAAATGGAAA TATAAATCAG
    ACAGACCAAC CTTCATCCAA GTGCTGTCCC TGTCCCAAAA CTGATTTCCA AATCCAGAAA
    GAACAGGATG AATCAGCATT CAGAAAAACA TTTGAAAATT ATCTACATAA TGAAGTTTTT
    ATACCTAGAC CTGTTCGAAA ACGGCGTGAA CTATTTGGAG TAGCCAATGG AACTCTGCCA
    GACCCAGTGA CAGCCCCTTC TCTCTTCAAT GCCTCCTCCA CCCGTGCTCC GGATGAAACA
    GAGCCAAAGA TATATTCACA GAAAGTTTGG TTTAAGGAGT CTCTAATGGT CACTGGCTTA
    AAGCATTTTA CTGGTTACCG CATTGAGATT CATGCCTGCA ACCACGAACT CTCCATGGGA
    TGTAGCGTGG CAGCATATGT TAGTGCAAGG ACAATGCCAG AGGCCACTGC CGACAATGTT
    GTGGGACCCA TCACCTATGA GTATATTGAA CCTAATATAA TCCATTTAAA ATGGCAAGAA
    CCAAAAGATC CAAATGGGCT AATAGTTTTG TATGAGGTGC ATTACTCCCG AGTTGGGGGC
    ACAGAGGAAG TGATTAGCTG CGTGTCACAG AAGCAGTATA GCACAGACAA AGGGGCAAAA
    CTCAGGGTAT TAACCCCAGG GAATTACAGT GTAAAGATCC GTGCAACCTC CCTGGCAGGC
    AATGGATCAT GGACAGAACC AGCCTACTTC CAAGTGCCAG ACCACCCTCA CAGCAGCATA
    GTGAAAATCG TCACTGGTCC AGTAATTGCA GTCATTCTTC TGCTTTTTGT CCTGGTTTAC
    TGTGTTATTC AGAAGAAAAA AGATGCAGAA GGCCCAGTTG GACCACTCTA CGCCTCCTCT
    AATCCTGAAT ATCTTAGTGC AAGTGAAGTC TACATCCCTG ACGAATGGGA GGTACCACGT
    GATAAAATCA GCTTACTGCG AGAATTGGGC CAGGGCTCAT TTGGAATGGT GTACGAGGGT
    ATTGCCAAAG ATATTATCAA GGGAGAGCCA GAAGTTCGTG TTGCTGTGAA AACTGTGAAT
    GAGTCTGCAA GCCTTCGAGA GAGGATAGAA TTCCTAAATG AGGCTTCTGT CATGAAGGCA
    TTTAGCTGCC ACCATGTGGT CCGTCTGCTT GGTGTGGTGT CCAAAGGTCA GCCAACACTT
    GTCATCATGG AGTTAATGAC GCATGGGGAT CTCAAAAGTT ACCTGCGCTC ACTGCGACCT
    GATGCAGAGA ATAACCCAGG ACGTCCTGCT CCTACCCTTA AAGAAATGAT CCAGATGGCA
    GCGGAAATTT CAGATGGCAT GGCATATCTC AATGCTAAAA AGTTTGTACA CAGGGATCTG
    GCTGCTAGGA ACTGCATGGT TGCTGAAGAT TATACTGTGA AAATTGGAGA TTTTGGAATG
    ACACGAGATA TCTATGAGAC AGATTATTAC CGAAAAGGGG GAAAGGGGCT GCTGCCTGTT
    AGATGGATGT CCCCTGAATC ACTGAAAGAT GGAGTGTTCA CAACTTTCTC AGATGTCTGG
    TCCTTTGGAG TTGTTTTATG GGAAATAACG AGTTTGGCAG AACAGCCATA TCAGGGACTT
    TCTAATGAGC AGGTCCTGAA GTTTGTCATG GATGGAGGTT CACTTGACCA ACCGGAGAAT
    TGCCCTCCAA GGCTGCATAG TCTGATGCAG ATGTGCTGGC AGTATAACCC CAAGATGCGG
    CCTACCTTCT TGGAGATTAT CGACATGTTA AAAGATGACC TGCATCTTAG TTTTCAGGAA
    GTCTCATTCT ACTACAGTGA CGAGAACAAG CCACCTGAAA CAGATGATTT GGAAATTGAT
    TTTGAAAATA TGGAGAGCAC ACCCCTGGAC CCTTCTTCCT GTTCTTTACG GGATCAGTCG
    AATCGGGGGA ACATTTATGA AGAACACATC CCCTACACCC ATATGAATGG TGGCAGAAAA
    AATGGACGTA TACTGTCTTT GCCACGGTCC AGCCCATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04156
    Clone ID Related Accession (Same CDS sequence) XM_002934379.5 , XM_002934379.4
    Accession Version XM_002934379.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4083bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product insulin receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934379.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGACAGG GTGTATTGTG GGGTGAGGGG CACCCCAATA ATAACCCCAA GAGCAAGGTG 
    GGCTGGAAGT CCATTGCGGG AATATTCATC ATTTTTATGC TCATTCTGTG CGACCAAAAC
    GACGGGAAAA TTTGCCCCAG TATGGATATC CGGAATAACA TTAGTCAGTT CAGTATGCTT
    GAGGACTGTA CAGTAATTGA AGGTCACCTT CAGATCTTGC TTATGTTCAC CAGCAAACCA
    GAGAACTTCC GAGGGTTGAG ATTTCCCAAG CTCACCATGA TCACAGATTA CCTCCTGCTT
    TTCCGGGTCT ATGGCTTAGA AAGTCTAAAG GACCTTTTTC CCAACCTCAC AGTTATCCGT
    GGTACTCACC TCTTTTTCAA CTATGCCCTA GTCATCTTTG AGATGGTCCA CCTGAAGGAG
    ATTGGGCTCT ACAACCTAAT GAACATTACT AGGGGGTCTG TTAGAATTGA GAAAAATAAT
    GAACTTTGTT ATCTTTCTAC GATTGATTGG TCCATAATTC TTGACTCTGT GGAGGATAAC
    TACATTGAAC TCAACAGGGA CAATAAAGAG GAGTGTGGAG ATGTTTGTCC TGGTGCAGGA
    AAAGGCAAGA GCAAATGCAA ACAGATAGTA GTCAATGGAG CATTCATTGA GCGCTGCTGG
    ACACAGGACC ACTGTCAAAC AGTGTGCCCT TCAGAATGCA AGGGAAGTGG TTGTTTGCCA
    GATGGACAGT GCTGCCACTC AGAGTGCCTT GGAAGCTGCA GAAAGCCTAA TGATCCAACT
    GAGTGTACCG CCTGCCGGCA TTACCAGTAT CAGGGTGTGT GTATCAGTGC ATGTCCCGAG
    GGGTCTTACC GGTTCCAGGG TTGGCGGTGC ATTGACTTCA ATACCTGTCA AGAAATGCAC
    AACCGGTGCC CAAATAAGGA AAATTCCTGT CCTCCTTATG TCATCCACAA GGGAGAGTGC
    ATGCCAGATT GCCCATCAGG CTATATTGCC AACTCAACTA CCCGTACTTG TACACCATGC
    GCAGGACCTT GCCCCAAAGT TTGCACCATC TACCAAAGTG TCAAGACCAT AGACTCTGTG
    ACATCTGCTC AAGAATTAAG AGGGTGCACA GTGGTCAACG GTAGTCTTAT CATCAACATC
    CGTGGAGGCA ATAATATTGC AACAGAATTA GAAGCTAACC TGGGCCTCAT TGAGGAGATT
    TCTGGATATC TTAAGATCAG AAGATCATAT GCATTGGTGT CTTTGTCATT CTTCCGCAAG
    CTGCGTTTAA TTAGGGGAGA AGTGTTGGAA GCAGGTAACT ATTCCTTCTA TGCTCTGGAT
    AATCCCAGTC TACGCCAGCT GTGGGACTGG CATAAGCACA ACCTGACCAT CATACATGGC
    AAGCTCTTCT TCCATCACAA CCCACGACTA TGTCTAGCGG AGATTCACCA AATGGAGGAG
    GTTACAGGAA CAAAGGGCCG GCAAGACAAG AATGATATAT CTACAAAGAC CAATGGAGAT
    CAGGCATCTT GTGAGGACAA TTTACTGAAC TTTAACTTTA TAAGGACATC GCATGATATG
    GTGCTGTTAA AGTGGGATCC TTACTGGCCC CCTGATTACA GAGACCTCTT GGGCTTCATG
    GTGCATTATA AGGAAGCACC CTACCAGAAT GTCACAGAAT TTGATGGTCA GGATGCTTGT
    GGTTCCAATA GTTGGACAGT GGTAGATATG GATGCCCCAG AACGAAGTGC TGATGGCAAG
    ACTCTATCTC CGGGATGTTT GCTCCGTAAC CTGAAACCAT GGACTCAGTA TGCAGTGTTT
    GTTAAAACAC TGGTATCCGG ATCAGATGAA GGAAGAACTT ATGGTGCAAA AAGCAAAATT
    ATCTATATTC GTACCAATGA AACCATCCCC TCTGTTCCTC TGGATCCTTT CTCTGTGTCC
    AACTCAACCT CTCAGATTAT TCTAAAATGG AAACCACCTT CTGAGCCCAA TGGAAATGTC
    ACTCACTACC TTGTGTACTG GCAAGAACAA CCTGAAGACA GTGAACTTTA TGAGCTGGAT
    TACTGCAATA AAGGTCTTAA GTTGCCTTCC AGAACTTGGA CTCCTCCCTC GGATATTGAG
    GAAAATGGAA ATATAAATCA GACAGACCAA CCTTCATCCA AGTGCTGTCC CTGTCCCAAA
    ACTGATTTCC AAATCCAGAA AGAACAGGAT GAATCAGCAT TCAGAAAAAC ATTTGAAAAT
    TATCTACATA ATGAAGTTTT TATACCTAGA CCTGTTCGAA AACGGCGTGA ACTATTTGGA
    GTAGCCAATG GAACTCTGCC AGACCCAGTG ACAGCCCCTT CTCTCTTCAA TGCCTCCTCC
    ACCCGTGCTC CGGATGAAAC AGAGCCAAAG ATATATTCAC AGAAAGTTTG GTTTAAGGAG
    TCTCTAATGG TCACTGGCTT AAAGCATTTT ACTGGTTACC GCATTGAGAT TCATGCCTGC
    AACCACGAAC TCTCCATGGG ATGTAGCGTG GCAGCATATG TTAGTGCAAG GACAATGCCA
    GAGGCCACTG CCGACAATGT TGTGGGACCC ATCACCTATG AGTATATTGA ACCTAATATA
    ATCCATTTAA AATGGCAAGA ACCAAAAGAT CCAAATGGGC TAATAGTTTT GTATGAGGTG
    CATTACTCCC GAGTTGGGGG CACAGAGGAA GTGATTAGCT GCGTGTCACA GAAGCAGTAT
    AGCACAGACA AAGGGGCAAA ACTCAGGGTA TTAACCCCAG GGAATTACAG TGTAAAGATC
    CGTGCAACCT CCCTGGCAGG CAATGGATCA TGGACAGAAC CAGCCTACTT CCAAGTGCCA
    GACCACCCTC ACAGCAGCAT AGTGAAAATC GTCACTGGTC CAGTAATTGC AGTCATTCTT
    CTGCTTTTTG TCCTGGTTTA CTGTGTTATT CAGAAGAAAA AAGATGCAGA AGGCCCAGTT
    GGACCACTCT ACGCCTCCTC TAATCCTGAA TATCTTAGTG CAAGTGAAGT CTACATCCCT
    GACGAATGGG AGGTACCACG TGATAAAATC AGCTTACTGC GAGAATTGGG CCAGGGCTCA
    TTTGGAATGG TGTACGAGGG TATTGCCAAA GATATTATCA AGGGAGAGCC AGAAGTTCGT
    GTTGCTGTGA AAACTGTGAA TGAGTCTGCA AGCCTTCGAG AGAGGATAGA ATTCCTAAAT
    GAGGCTTCTG TCATGAAGGC ATTTAGCTGC CACCATGTGG TCCGTCTGCT TGGTGTGGTG
    TCCAAAGGTC AGCCAACACT TGTCATCATG GAGTTAATGA CGCATGGGGA TCTCAAAAGT
    TACCTGCGCT CACTGCGACC TGATGCAGAG AATAACCCAG GACGTCCTGC TCCTACCCTT
    AAAGAAATGA TCCAGATGGC AGCGGAAATT TCAGATGGCA TGGCATATCT CAATGCTAAA
    AAGTTTGTAC ACAGGGATCT GGCTGCTAGG AACTGCATGG TTGCTGAAGA TTATACTGTG
    AAAATTGGAG ATTTTGGAAT GACACGAGAT ATCTATGAGA CAGATTATTA CCGAAAAGGG
    GGAAAGGGGC TGCTGCCTGT TAGATGGATG TCCCCTGAAT CACTGAAAGA TGGAGTGTTC
    ACAACTTTCT CAGATGTCTG GTCCTTTGGA GTTGTTTTAT GGGAAATAAC GAGTTTGGCA
    GAACAGCCAT ATCAGGGACT TTCTAATGAG CAGGTCCTGA AGTTTGTCAT GGATGGAGGT
    TCACTTGACC AACCGGAGAA TTGCCCTCCA AGGCTGCATA GTCTGATGCA GATGTGCTGG
    CAGTATAACC CCAAGATGCG GCCTACCTTC TTGGAGATTA TCGACATGTT AAAAGATGAC
    CTGCATCTTA GTTTTCAGGA AGTCTCATTC TACTACAGTG ACGAGAACAA GCCACCTGAA
    ACAGATGATT TGGAAATTGA TTTTGAAAAT ATGGAGAGCA CACCCCTGGA CCCTTCTTCC
    TGTTCTTTAC GGGATCAGTC GAATCGGGGG AACATTTATG AAGAACACAT CCCCTACACC
    CATATGAATG GTGGCAGAAA AAATGGACGT ATACTGTCTT TGCCACGGTC CAGCCCATCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934425.2
    CDS256..4338
    Translation

    Target ORF information:

    RefSeq Version XM_002934379.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis insulin receptor (insr), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934379.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGACAGG GTGTATTGTG GGGTGAGGGG CACCCCAATA ATAACCCCAA GAGCAAGGTG 
    GGCTGGAAGT CCATTGCGGG AATATTCATC ATTTTTATGC TCATTCTGTG CGACCAAAAC
    GACGGGAAAA TTTGCCCCAG TATGGATATC CGGAATAACA TTAGTCAGTT CAGTATGCTT
    GAGGACTGTA CAGTAATTGA AGGTCACCTT CAGATCTTGC TTATGTTCAC CAGCAAACCA
    GAGAACTTCC GAGGGTTGAG ATTTCCCAAG CTCACCATGA TCACAGATTA CCTCCTGCTT
    TTCCGGGTCT ATGGCTTAGA AAGTCTAAAG GACCTTTTTC CCAACCTCAC AGTTATCCGT
    GGTACTCACC TCTTTTTCAA CTATGCCCTA GTCATCTTTG AGATGGTCCA CCTGAAGGAG
    ATTGGGCTCT ACAACCTAAT GAACATTACT AGGGGGTCTG TTAGAATTGA GAAAAATAAT
    GAACTTTGTT ATCTTTCTAC GATTGATTGG TCCATAATTC TTGACTCTGT GGAGGATAAC
    TACATTGAAC TCAACAGGGA CAATAAAGAG GAGTGTGGAG ATGTTTGTCC TGGTGCAGGA
    AAAGGCAAGA GCAAATGCAA ACAGATAGTA GTCAATGGAG CATTCATTGA GCGCTGCTGG
    ACACAGGACC ACTGTCAAAC AGTGTGCCCT TCAGAATGCA AGGGAAGTGG TTGTTTGCCA
    GATGGACAGT GCTGCCACTC AGAGTGCCTT GGAAGCTGCA GAAAGCCTAA TGATCCAACT
    GAGTGTACCG CCTGCCGGCA TTACCAGTAT CAGGGTGTGT GTATCAGTGC ATGTCCCGAG
    GGGTCTTACC GGTTCCAGGG TTGGCGGTGC ATTGACTTCA ATACCTGTCA AGAAATGCAC
    AACCGGTGCC CAAATAAGGA AAATTCCTGT CCTCCTTATG TCATCCACAA GGGAGAGTGC
    ATGCCAGATT GCCCATCAGG CTATATTGCC AACTCAACTA CCCGTACTTG TACACCATGC
    GCAGGACCTT GCCCCAAAGT TTGCACCATC TACCAAAGTG TCAAGACCAT AGACTCTGTG
    ACATCTGCTC AAGAATTAAG AGGGTGCACA GTGGTCAACG GTAGTCTTAT CATCAACATC
    CGTGGAGGCA ATAATATTGC AACAGAATTA GAAGCTAACC TGGGCCTCAT TGAGGAGATT
    TCTGGATATC TTAAGATCAG AAGATCATAT GCATTGGTGT CTTTGTCATT CTTCCGCAAG
    CTGCGTTTAA TTAGGGGAGA AGTGTTGGAA GCAGGTAACT ATTCCTTCTA TGCTCTGGAT
    AATCCCAGTC TACGCCAGCT GTGGGACTGG CATAAGCACA ACCTGACCAT CATACATGGC
    AAGCTCTTCT TCCATCACAA CCCACGACTA TGTCTAGCGG AGATTCACCA AATGGAGGAG
    GTTACAGGAA CAAAGGGCCG GCAAGACAAG AATGATATAT CTACAAAGAC CAATGGAGAT
    CAGGCATCTT GTGAGGACAA TTTACTGAAC TTTAACTTTA TAAGGACATC GCATGATATG
    GTGCTGTTAA AGTGGGATCC TTACTGGCCC CCTGATTACA GAGACCTCTT GGGCTTCATG
    GTGCATTATA AGGAAGCACC CTACCAGAAT GTCACAGAAT TTGATGGTCA GGATGCTTGT
    GGTTCCAATA GTTGGACAGT GGTAGATATG GATGCCCCAG AACGAAGTGC TGATGGCAAG
    ACTCTATCTC CGGGATGTTT GCTCCGTAAC CTGAAACCAT GGACTCAGTA TGCAGTGTTT
    GTTAAAACAC TGGTATCCGG ATCAGATGAA GGAAGAACTT ATGGTGCAAA AAGCAAAATT
    ATCTATATTC GTACCAATGA AACCATCCCC TCTGTTCCTC TGGATCCTTT CTCTGTGTCC
    AACTCAACCT CTCAGATTAT TCTAAAATGG AAACCACCTT CTGAGCCCAA TGGAAATGTC
    ACTCACTACC TTGTGTACTG GCAAGAACAA CCTGAAGACA GTGAACTTTA TGAGCTGGAT
    TACTGCAATA AAGGTCTTAA GTTGCCTTCC AGAACTTGGA CTCCTCCCTC GGATATTGAG
    GAAAATGGAA ATATAAATCA GACAGACCAA CCTTCATCCA AGTGCTGTCC CTGTCCCAAA
    ACTGATTTCC AAATCCAGAA AGAACAGGAT GAATCAGCAT TCAGAAAAAC ATTTGAAAAT
    TATCTACATA ATGAAGTTTT TATACCTAGA CCTGTTCGAA AACGGCGTGA ACTATTTGGA
    GTAGCCAATG GAACTCTGCC AGACCCAGTG ACAGCCCCTT CTCTCTTCAA TGCCTCCTCC
    ACCCGTGCTC CGGATGAAAC AGAGCCAAAG ATATATTCAC AGAAAGTTTG GTTTAAGGAG
    TCTCTAATGG TCACTGGCTT AAAGCATTTT ACTGGTTACC GCATTGAGAT TCATGCCTGC
    AACCACGAAC TCTCCATGGG ATGTAGCGTG GCAGCATATG TTAGTGCAAG GACAATGCCA
    GAGGCCACTG CCGACAATGT TGTGGGACCC ATCACCTATG AGTATATTGA ACCTAATATA
    ATCCATTTAA AATGGCAAGA ACCAAAAGAT CCAAATGGGC TAATAGTTTT GTATGAGGTG
    CATTACTCCC GAGTTGGGGG CACAGAGGAA GTGATTAGCT GCGTGTCACA GAAGCAGTAT
    AGCACAGACA AAGGGGCAAA ACTCAGGGTA TTAACCCCAG GGAATTACAG TGTAAAGATC
    CGTGCAACCT CCCTGGCAGG CAATGGATCA TGGACAGAAC CAGCCTACTT CCAAGTGCCA
    GACCACCCTC ACAGCAGCAT AGTGAAAATC GTCACTGGTC CAGTAATTGC AGTCATTCTT
    CTGCTTTTTG TCCTGGTTTA CTGTGTTATT CAGAAGAAAA AAGATGCAGA AGGCCCAGTT
    GGACCACTCT ACGCCTCCTC TAATCCTGAA TATCTTAGTG CAAGTGAAGT CTACATCCCT
    GACGAATGGG AGGTACCACG TGATAAAATC AGCTTACTGC GAGAATTGGG CCAGGGCTCA
    TTTGGAATGG TGTACGAGGG TATTGCCAAA GATATTATCA AGGGAGAGCC AGAAGTTCGT
    GTTGCTGTGA AAACTGTGAA TGAGTCTGCA AGCCTTCGAG AGAGGATAGA ATTCCTAAAT
    GAGGCTTCTG TCATGAAGGC ATTTAGCTGC CACCATGTGG TCCGTCTGCT TGGTGTGGTG
    TCCAAAGGTC AGCCAACACT TGTCATCATG GAGTTAATGA CGCATGGGGA TCTCAAAAGT
    TACCTGCGCT CACTGCGACC TGATGCAGAG AATAACCCAG GACGTCCTGC TCCTACCCTT
    AAAGAAATGA TCCAGATGGC AGCGGAAATT TCAGATGGCA TGGCATATCT CAATGCTAAA
    AAGTTTGTAC ACAGGGATCT GGCTGCTAGG AACTGCATGG TTGCTGAAGA TTATACTGTG
    AAAATTGGAG ATTTTGGAAT GACACGAGAT ATCTATGAGA CAGATTATTA CCGAAAAGGG
    GGAAAGGGGC TGCTGCCTGT TAGATGGATG TCCCCTGAAT CACTGAAAGA TGGAGTGTTC
    ACAACTTTCT CAGATGTCTG GTCCTTTGGA GTTGTTTTAT GGGAAATAAC GAGTTTGGCA
    GAACAGCCAT ATCAGGGACT TTCTAATGAG CAGGTCCTGA AGTTTGTCAT GGATGGAGGT
    TCACTTGACC AACCGGAGAA TTGCCCTCCA AGGCTGCATA GTCTGATGCA GATGTGCTGG
    CAGTATAACC CCAAGATGCG GCCTACCTTC TTGGAGATTA TCGACATGTT AAAAGATGAC
    CTGCATCTTA GTTTTCAGGA AGTCTCATTC TACTACAGTG ACGAGAACAA GCCACCTGAA
    ACAGATGATT TGGAAATTGA TTTTGAAAAT ATGGAGAGCA CACCCCTGGA CCCTTCTTCC
    TGTTCTTTAC GGGATCAGTC GAATCGGGGG AACATTTATG AAGAACACAT CCCCTACACC
    CATATGAATG GTGGCAGAAA AAATGGACGT ATACTGTCTT TGCCACGGTC CAGCCCATCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.