rgs22 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following rgs22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rgs22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa11471 XM_002934418.5
Latest version!
Xenopus tropicalis regulator of G-protein signaling 22 (rgs22), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa11471 XM_018095160.2
Latest version!
Xenopus tropicalis regulator of G-protein signaling 22 (rgs22), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OXa11471 XM_018095160.1 Xenopus tropicalis regulator of G-protein signaling 22 (rgs22), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa11471 XM_002934418.4 Xenopus tropicalis regulator of G-protein signaling 22 (rgs22), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa11471
    Clone ID Related Accession (Same CDS sequence) XM_018095160.1 , XM_018095160.2 , XM_002934418.5 , XM_002934418.4
    Accession Version XM_018095160.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulator of G-protein signaling 22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095160.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGTGCA AAAGGCTAGC AACTGTGCCC CCTGATATAA AAGAAGATGA TTTTGAGGAC 
    TTCTTAGCAA CAGATGACTT GCTTGTAGAT TACTTCAATG AATTTCTAAG CCTGCCGACC
    TTTCCAGAAC CAATCAAGTT CAACAAAACT TTTGGCATCT TCGAAGTTGC AAATAATGCA
    GGGGAACACC TAAAACAGCA GATAAAACAA GTGTTACAGG ACCTAGAATC TCCAAATCCC
    ATTTATGATG CTACCAGAAG AGTAACTGGC TTGAAACCTA GGGATCCTCC TCCTCCTGAC
    CTAAACATTG ATATTAATTA CAGTATTATG TGCTTGAATC GAGAGCAAGG GGTTCAGTGG
    ATAAAGAGAG AGAGACTACC GGCTTTCCTA AAAAGTGATT GCTATTTTGA GTACAGACTT
    GCTAAGCTGC TGGCACAGGT AAGAGCGAGC AACTCTGGAG TGACGGTTCT TGCTGACCCA
    AAGTACCAAC CATGGCAACT TAGGAAAGAG AGTGCTCCTT TGCCAAAAAC AGAAAACGAA
    GACCACAGCA TCATGCAGAA ATTCTTTGTC ACACTTGGCC AGGCTTCGTT TACTCAGACA
    AAGGAATGGT TTTCAGTAGC AAAGGACAGT GTTCAAACCA CTACAACTGA TTCATTAAAT
    GGCACAGTCG AGTGCAGAAC TGAAATTACC AGTACTAGAA TGAATTCACA GATTAGTAAA
    GCCTCAGAAG GTAGTCATAA TCAGGGAGTC CCTTGCAGGC CACTTTCTGC AAAATATCAT
    AATGGAAGTG AGATTGCTGT AGATGAAGAA GATGAGTATG CTGTATCTAT CACAGATGCA
    CCATGTCAAA CCCCTGCGAG GATTTATATT AATCAGGGGT CAGATAATTA TGAAGCACAA
    AACCAGAAAG CAAATGACTC TCAAGGCCTG CAGTCTGAAA ATTCACCAGT ATTTCCCACA
    GTGGAAGATT TTGCTCGTTC CTATGTTGAG CATGTTATGA AATCTGCAGT TTTAGAGCTA
    AGTGGGCAAC AAGTCCAGCA AAGTAACAAG GACCTTGATA TTCATGCTTT ATCTGCAGTG
    ACTATCCGTA GCTTGCAGAG TTCCGATACT TTATTTAGCT CCAAGAGCTC GGAAACGTCC
    ATTCAAGAGG AGATCATTAT TTCAGAGGCT GACAGTGATG AACTTGACAC CGCTGACTCC
    TTCTGTCTCA GAGACACTGA CTATCACATC AAGACCCGAC GGGAGTTTGA AATGTTTAAA
    TCATTTCTTA AAGGAACTTC AGGGGAGAAG TTATGGTGCC TCTGGATAGA CATTGAAAGG
    CTTCAAGCCA TAAAAGACCA CAAAAGGCAA CAGGGTCATC TGAATAAGAT GAAGAAACGC
    TATCTTGTGA CGAGCGGGGA CCTCTTTTTT ACAAGAGAAT TACTGTTTAA ACTGGAACTT
    TTGAATACAA ATCACTGGAA CAAAAATCAT TTGCAAAATG TGCAGAGTGA GGTGGTTAAA
    CCATTGCTTC TGTATTGGGG GCCTAGATTC TGTATGTCTG ATTCTGCTTT GGCTTATAAT
    GTTCACGCGG AGCTGAAAGC CTGGTCTGAT CGTCAGCTGA GACCCAAAAG AGATGCTGAC
    CCTTTTGCAA AGACGGTAAC GCTGTTACCA TTGAGACCCA AGTCTTGCGC CCCAAGAATT
    AGTTCATCAG TTCATCGTTT ACTTGAACAG AGCCCACCTC TAAAATCTTC AAAAAGAAGT
    AAGACGTGTT CTCCAAGTCG ACAATCCGGC AGCTTGATTT TTGCAAACAT AAAACCAAAA
    CAAACCAGCA GGAGTTTCCC TTGCAGTGCG GCTTATAATT CTCCATCTAG CATGAAACGA
    AAACATCAAA GAACAAACAG CTATCTGTCT GTGTCCCTGG GTTTGCCTAG AGAGAGTTTA
    AATATAGGAT CAGTGTATGA TCAAGATGTT CCCAAGGATG ATTTGAGTGA GGATGGTAGT
    ACCACCAGCG TATTAAGGGG CTCCAATATG GCAGAGATGT TAAATGCTCT GAATCTGGAC
    TCTCGAGCTG GCTATTTCTT TGCTACGTTC TGTGAGAAGT CAGGGAACAA GTTGTGGGAG
    AATGGGATCT ATTTCTGGTT TGATCTACAG TCCTATCATC ACAAGTTTTA CCAGGAGACA
    CTCCAACCAT TTCAGCTATG CAGACATTCC CAATTTCTGT TTGGCACGTA CCTTGCGCCT
    TCTGCTTCCA TGGATATCGG AGCCGAGCAG AACATTAAAA AAGAGATTTA CCAGAAACTT
    GATCCACCTT TTGAGGATTT ATTTGACTCG GCTGAAGAAT ACATTCTTAC ACTCCTGCTC
    TCTGCCTGGA GTCAGATGAC AGAGTCAGAC AGACAGGTCT ACAGCAAGGT GGAGCTGGTG
    GAGGAATCTC GGCAGCTGGA ATCACCACTC TACAAAAAGC TGCAAGCTTT GCAGCAGGAG
    AGAATGGCCA AGAAAAATCA AGATCAGATA TACAAATCCA TTTTCCTACC ACCTCCTGAC
    ATTGCCAAAG AGCCTGACCT CTGGGAACTG GTCCCTGAGG AATACAGGAA TTATAACCTC
    ACCACCTTAA TTCGACACCG GATGGAGCTT GAGCATTTCC GCAATTTTCT GGAAGATCAT
    TTTGCGAGCA TGGATCTCAT GTGCTGGATA GACCTGGAGC AGCTGAGGAG AATGCCACAC
    AAAGAAAAGG AGAAACGCCA GGAAAAATCC AAGGACATTA AAGACAAATA TTTAAACAAG
    AAGTACTTTT TTGGTCCAAA CAGTCCAGCC ACCAGAGATC AGCAGGAACA GGTAATGAAA
    CTAGCAGGAG GCTGGGGCAA GATTCTACAT GATCGACTGT CGGCGGCGGT CCTTATAGAA
    GTACAGAAAT ACGTCAGAAT GAGGATAGAG AGAAAGTGGC TTCCAATGTT TTTAGCAACA
    GAGGAGTACA GAGAGCGCCA GAGAGTGCAA GGACAAATGA AAGACGTTGC AGAAGATGTA
    ATATTTCAAA CAAACAAAAA GAAAATGGGA GTGTGGAAGC ATCTTGACAG TAAATGGGTC
    TCATCCTCCA AAGAGATAAT TGCCTTTCGT AAAGCCTTAA TGAATCCAGT AACTTCCAGT
    CAGTTTCAGC GCTTCATTTC ACTCAAAGGA GACTTTTTGG AGAATGGACT GCTTTTTTGG
    ATTGAAGTAC AGAAATACAA GGATCTCTGC CACTCCCACT GTGATGATTT CACAATTCAG
    AATAAAATTA CTGCTATCAT TAATTGCTTC ATCAACTCCT CCATCCCACC AGCCTTGCAG
    ATTGACATTC CCCAAGAGCA AGCAGAGAAA ATCTTAGAGC GCCGCCGGGA TTTAGGCCCA
    TATGTATTCC GTGAGGCTCA GATGACCGTC TTCAGCATCA TGTTCAAATT CTGGCCGGAG
    TTTTGTGATT TCCGTAGAAA TTTGACAGAA GAAAAGATTT TACCTTTTCT GGATAGAAAG
    AAATTAAAGC AGACAGAGGC AGTGAAAAGA AGAATGAGAG AAGAAGAGAG ACTTGCAAAG
    CAACAGCAAG AGGAGGCTAA GAGGAAAGGA ACATTATCCG ACTTGTATGG TGATAACGAA
    AGCACTTACA GCGGGGACAG GTTATCTGGA TACGGGGGAC ATGCACGGGA TGGATTTGCA
    CATGATGGAT ATGTGGCAGA TGGGTTTGGA CAAAGCAGGA ATGTATCTTG GAGTTATTCT
    AAGTATGTTG AAGCTTTGGA GAAGGAAAGG ATGCTTCTGA AAATTCAGGA AGATCTTGAA
    AAAAAGTCGC GGAGCTCCCT TCTGTCTGAT ATTTCGTCTC TTTACAGTGT AAGAACAGAA
    GCCTCAAAAA GAACCAACAG ATCTTCATCC GCACCCACTG ACAGGCCTTC CTCTGTTACA
    TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950649.1
    CDS376..4281
    Translation

    Target ORF information:

    RefSeq Version XM_018095160.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulator of G-protein signaling 22 (rgs22), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095160.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGTGCA AAAGGCTAGC AACTGTGCCC CCTGATATAA AAGAAGATGA TTTTGAGGAC 
    TTCTTAGCAA CAGATGACTT GCTTGTAGAT TACTTCAATG AATTTCTAAG CCTGCCGACC
    TTTCCAGAAC CAATCAAGTT CAACAAAACT TTTGGCATCT TCGAAGTTGC AAATAATGCA
    GGGGAACACC TAAAACAGCA GATAAAACAA GTGTTACAGG ACCTAGAATC TCCAAATCCC
    ATTTATGATG CTACCAGAAG AGTAACTGGC TTGAAACCTA GGGATCCTCC TCCTCCTGAC
    CTAAACATTG ATATTAATTA CAGTATTATG TGCTTGAATC GAGAGCAAGG GGTTCAGTGG
    ATAAAGAGAG AGAGACTACC GGCTTTCCTA AAAAGTGATT GCTATTTTGA GTACAGACTT
    GCTAAGCTGC TGGCACAGGT AAGAGCGAGC AACTCTGGAG TGACGGTTCT TGCTGACCCA
    AAGTACCAAC CATGGCAACT TAGGAAAGAG AGTGCTCCTT TGCCAAAAAC AGAAAACGAA
    GACCACAGCA TCATGCAGAA ATTCTTTGTC ACACTTGGCC AGGCTTCGTT TACTCAGACA
    AAGGAATGGT TTTCAGTAGC AAAGGACAGT GTTCAAACCA CTACAACTGA TTCATTAAAT
    GGCACAGTCG AGTGCAGAAC TGAAATTACC AGTACTAGAA TGAATTCACA GATTAGTAAA
    GCCTCAGAAG GTAGTCATAA TCAGGGAGTC CCTTGCAGGC CACTTTCTGC AAAATATCAT
    AATGGAAGTG AGATTGCTGT AGATGAAGAA GATGAGTATG CTGTATCTAT CACAGATGCA
    CCATGTCAAA CCCCTGCGAG GATTTATATT AATCAGGGGT CAGATAATTA TGAAGCACAA
    AACCAGAAAG CAAATGACTC TCAAGGCCTG CAGTCTGAAA ATTCACCAGT ATTTCCCACA
    GTGGAAGATT TTGCTCGTTC CTATGTTGAG CATGTTATGA AATCTGCAGT TTTAGAGCTA
    AGTGGGCAAC AAGTCCAGCA AAGTAACAAG GACCTTGATA TTCATGCTTT ATCTGCAGTG
    ACTATCCGTA GCTTGCAGAG TTCCGATACT TTATTTAGCT CCAAGAGCTC GGAAACGTCC
    ATTCAAGAGG AGATCATTAT TTCAGAGGCT GACAGTGATG AACTTGACAC CGCTGACTCC
    TTCTGTCTCA GAGACACTGA CTATCACATC AAGACCCGAC GGGAGTTTGA AATGTTTAAA
    TCATTTCTTA AAGGAACTTC AGGGGAGAAG TTATGGTGCC TCTGGATAGA CATTGAAAGG
    CTTCAAGCCA TAAAAGACCA CAAAAGGCAA CAGGGTCATC TGAATAAGAT GAAGAAACGC
    TATCTTGTGA CGAGCGGGGA CCTCTTTTTT ACAAGAGAAT TACTGTTTAA ACTGGAACTT
    TTGAATACAA ATCACTGGAA CAAAAATCAT TTGCAAAATG TGCAGAGTGA GGTGGTTAAA
    CCATTGCTTC TGTATTGGGG GCCTAGATTC TGTATGTCTG ATTCTGCTTT GGCTTATAAT
    GTTCACGCGG AGCTGAAAGC CTGGTCTGAT CGTCAGCTGA GACCCAAAAG AGATGCTGAC
    CCTTTTGCAA AGACGGTAAC GCTGTTACCA TTGAGACCCA AGTCTTGCGC CCCAAGAATT
    AGTTCATCAG TTCATCGTTT ACTTGAACAG AGCCCACCTC TAAAATCTTC AAAAAGAAGT
    AAGACGTGTT CTCCAAGTCG ACAATCCGGC AGCTTGATTT TTGCAAACAT AAAACCAAAA
    CAAACCAGCA GGAGTTTCCC TTGCAGTGCG GCTTATAATT CTCCATCTAG CATGAAACGA
    AAACATCAAA GAACAAACAG CTATCTGTCT GTGTCCCTGG GTTTGCCTAG AGAGAGTTTA
    AATATAGGAT CAGTGTATGA TCAAGATGTT CCCAAGGATG ATTTGAGTGA GGATGGTAGT
    ACCACCAGCG TATTAAGGGG CTCCAATATG GCAGAGATGT TAAATGCTCT GAATCTGGAC
    TCTCGAGCTG GCTATTTCTT TGCTACGTTC TGTGAGAAGT CAGGGAACAA GTTGTGGGAG
    AATGGGATCT ATTTCTGGTT TGATCTACAG TCCTATCATC ACAAGTTTTA CCAGGAGACA
    CTCCAACCAT TTCAGCTATG CAGACATTCC CAATTTCTGT TTGGCACGTA CCTTGCGCCT
    TCTGCTTCCA TGGATATCGG AGCCGAGCAG AACATTAAAA AAGAGATTTA CCAGAAACTT
    GATCCACCTT TTGAGGATTT ATTTGACTCG GCTGAAGAAT ACATTCTTAC ACTCCTGCTC
    TCTGCCTGGA GTCAGATGAC AGAGTCAGAC AGACAGGTCT ACAGCAAGGT GGAGCTGGTG
    GAGGAATCTC GGCAGCTGGA ATCACCACTC TACAAAAAGC TGCAAGCTTT GCAGCAGGAG
    AGAATGGCCA AGAAAAATCA AGATCAGATA TACAAATCCA TTTTCCTACC ACCTCCTGAC
    ATTGCCAAAG AGCCTGACCT CTGGGAACTG GTCCCTGAGG AATACAGGAA TTATAACCTC
    ACCACCTTAA TTCGACACCG GATGGAGCTT GAGCATTTCC GCAATTTTCT GGAAGATCAT
    TTTGCGAGCA TGGATCTCAT GTGCTGGATA GACCTGGAGC AGCTGAGGAG AATGCCACAC
    AAAGAAAAGG AGAAACGCCA GGAAAAATCC AAGGACATTA AAGACAAATA TTTAAACAAG
    AAGTACTTTT TTGGTCCAAA CAGTCCAGCC ACCAGAGATC AGCAGGAACA GGTAATGAAA
    CTAGCAGGAG GCTGGGGCAA GATTCTACAT GATCGACTGT CGGCGGCGGT CCTTATAGAA
    GTACAGAAAT ACGTCAGAAT GAGGATAGAG AGAAAGTGGC TTCCAATGTT TTTAGCAACA
    GAGGAGTACA GAGAGCGCCA GAGAGTGCAA GGACAAATGA AAGACGTTGC AGAAGATGTA
    ATATTTCAAA CAAACAAAAA GAAAATGGGA GTGTGGAAGC ATCTTGACAG TAAATGGGTC
    TCATCCTCCA AAGAGATAAT TGCCTTTCGT AAAGCCTTAA TGAATCCAGT AACTTCCAGT
    CAGTTTCAGC GCTTCATTTC ACTCAAAGGA GACTTTTTGG AGAATGGACT GCTTTTTTGG
    ATTGAAGTAC AGAAATACAA GGATCTCTGC CACTCCCACT GTGATGATTT CACAATTCAG
    AATAAAATTA CTGCTATCAT TAATTGCTTC ATCAACTCCT CCATCCCACC AGCCTTGCAG
    ATTGACATTC CCCAAGAGCA AGCAGAGAAA ATCTTAGAGC GCCGCCGGGA TTTAGGCCCA
    TATGTATTCC GTGAGGCTCA GATGACCGTC TTCAGCATCA TGTTCAAATT CTGGCCGGAG
    TTTTGTGATT TCCGTAGAAA TTTGACAGAA GAAAAGATTT TACCTTTTCT GGATAGAAAG
    AAATTAAAGC AGACAGAGGC AGTGAAAAGA AGAATGAGAG AAGAAGAGAG ACTTGCAAAG
    CAACAGCAAG AGGAGGCTAA GAGGAAAGGA ACATTATCCG ACTTGTATGG TGATAACGAA
    AGCACTTACA GCGGGGACAG GTTATCTGGA TACGGGGGAC ATGCACGGGA TGGATTTGCA
    CATGATGGAT ATGTGGCAGA TGGGTTTGGA CAAAGCAGGA ATGTATCTTG GAGTTATTCT
    AAGTATGTTG AAGCTTTGGA GAAGGAAAGG ATGCTTCTGA AAATTCAGGA AGATCTTGAA
    AAAAAGTCGC GGAGCTCCCT TCTGTCTGAT ATTTCGTCTC TTTACAGTGT AAGAACAGAA
    GCCTCAAAAA GAACCAACAG ATCTTCATCC GCACCCACTG ACAGGCCTTC CTCTGTTACA
    TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11471
    Clone ID Related Accession (Same CDS sequence) XM_018095160.1 , XM_018095160.2 , XM_002934418.5 , XM_002934418.4
    Accession Version XM_002934418.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulator of G-protein signaling 22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934418.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGTGCA AAAGGCTAGC AACTGTGCCC CCTGATATAA AAGAAGATGA TTTTGAGGAC 
    TTCTTAGCAA CAGATGACTT GCTTGTAGAT TACTTCAATG AATTTCTAAG CCTGCCGACC
    TTTCCAGAAC CAATCAAGTT CAACAAAACT TTTGGCATCT TCGAAGTTGC AAATAATGCA
    GGGGAACACC TAAAACAGCA GATAAAACAA GTGTTACAGG ACCTAGAATC TCCAAATCCC
    ATTTATGATG CTACCAGAAG AGTAACTGGC TTGAAACCTA GGGATCCTCC TCCTCCTGAC
    CTAAACATTG ATATTAATTA CAGTATTATG TGCTTGAATC GAGAGCAAGG GGTTCAGTGG
    ATAAAGAGAG AGAGACTACC GGCTTTCCTA AAAAGTGATT GCTATTTTGA GTACAGACTT
    GCTAAGCTGC TGGCACAGGT AAGAGCGAGC AACTCTGGAG TGACGGTTCT TGCTGACCCA
    AAGTACCAAC CATGGCAACT TAGGAAAGAG AGTGCTCCTT TGCCAAAAAC AGAAAACGAA
    GACCACAGCA TCATGCAGAA ATTCTTTGTC ACACTTGGCC AGGCTTCGTT TACTCAGACA
    AAGGAATGGT TTTCAGTAGC AAAGGACAGT GTTCAAACCA CTACAACTGA TTCATTAAAT
    GGCACAGTCG AGTGCAGAAC TGAAATTACC AGTACTAGAA TGAATTCACA GATTAGTAAA
    GCCTCAGAAG GTAGTCATAA TCAGGGAGTC CCTTGCAGGC CACTTTCTGC AAAATATCAT
    AATGGAAGTG AGATTGCTGT AGATGAAGAA GATGAGTATG CTGTATCTAT CACAGATGCA
    CCATGTCAAA CCCCTGCGAG GATTTATATT AATCAGGGGT CAGATAATTA TGAAGCACAA
    AACCAGAAAG CAAATGACTC TCAAGGCCTG CAGTCTGAAA ATTCACCAGT ATTTCCCACA
    GTGGAAGATT TTGCTCGTTC CTATGTTGAG CATGTTATGA AATCTGCAGT TTTAGAGCTA
    AGTGGGCAAC AAGTCCAGCA AAGTAACAAG GACCTTGATA TTCATGCTTT ATCTGCAGTG
    ACTATCCGTA GCTTGCAGAG TTCCGATACT TTATTTAGCT CCAAGAGCTC GGAAACGTCC
    ATTCAAGAGG AGATCATTAT TTCAGAGGCT GACAGTGATG AACTTGACAC CGCTGACTCC
    TTCTGTCTCA GAGACACTGA CTATCACATC AAGACCCGAC GGGAGTTTGA AATGTTTAAA
    TCATTTCTTA AAGGAACTTC AGGGGAGAAG TTATGGTGCC TCTGGATAGA CATTGAAAGG
    CTTCAAGCCA TAAAAGACCA CAAAAGGCAA CAGGGTCATC TGAATAAGAT GAAGAAACGC
    TATCTTGTGA CGAGCGGGGA CCTCTTTTTT ACAAGAGAAT TACTGTTTAA ACTGGAACTT
    TTGAATACAA ATCACTGGAA CAAAAATCAT TTGCAAAATG TGCAGAGTGA GGTGGTTAAA
    CCATTGCTTC TGTATTGGGG GCCTAGATTC TGTATGTCTG ATTCTGCTTT GGCTTATAAT
    GTTCACGCGG AGCTGAAAGC CTGGTCTGAT CGTCAGCTGA GACCCAAAAG AGATGCTGAC
    CCTTTTGCAA AGACGGTAAC GCTGTTACCA TTGAGACCCA AGTCTTGCGC CCCAAGAATT
    AGTTCATCAG TTCATCGTTT ACTTGAACAG AGCCCACCTC TAAAATCTTC AAAAAGAAGT
    AAGACGTGTT CTCCAAGTCG ACAATCCGGC AGCTTGATTT TTGCAAACAT AAAACCAAAA
    CAAACCAGCA GGAGTTTCCC TTGCAGTGCG GCTTATAATT CTCCATCTAG CATGAAACGA
    AAACATCAAA GAACAAACAG CTATCTGTCT GTGTCCCTGG GTTTGCCTAG AGAGAGTTTA
    AATATAGGAT CAGTGTATGA TCAAGATGTT CCCAAGGATG ATTTGAGTGA GGATGGTAGT
    ACCACCAGCG TATTAAGGGG CTCCAATATG GCAGAGATGT TAAATGCTCT GAATCTGGAC
    TCTCGAGCTG GCTATTTCTT TGCTACGTTC TGTGAGAAGT CAGGGAACAA GTTGTGGGAG
    AATGGGATCT ATTTCTGGTT TGATCTACAG TCCTATCATC ACAAGTTTTA CCAGGAGACA
    CTCCAACCAT TTCAGCTATG CAGACATTCC CAATTTCTGT TTGGCACGTA CCTTGCGCCT
    TCTGCTTCCA TGGATATCGG AGCCGAGCAG AACATTAAAA AAGAGATTTA CCAGAAACTT
    GATCCACCTT TTGAGGATTT ATTTGACTCG GCTGAAGAAT ACATTCTTAC ACTCCTGCTC
    TCTGCCTGGA GTCAGATGAC AGAGTCAGAC AGACAGGTCT ACAGCAAGGT GGAGCTGGTG
    GAGGAATCTC GGCAGCTGGA ATCACCACTC TACAAAAAGC TGCAAGCTTT GCAGCAGGAG
    AGAATGGCCA AGAAAAATCA AGATCAGATA TACAAATCCA TTTTCCTACC ACCTCCTGAC
    ATTGCCAAAG AGCCTGACCT CTGGGAACTG GTCCCTGAGG AATACAGGAA TTATAACCTC
    ACCACCTTAA TTCGACACCG GATGGAGCTT GAGCATTTCC GCAATTTTCT GGAAGATCAT
    TTTGCGAGCA TGGATCTCAT GTGCTGGATA GACCTGGAGC AGCTGAGGAG AATGCCACAC
    AAAGAAAAGG AGAAACGCCA GGAAAAATCC AAGGACATTA AAGACAAATA TTTAAACAAG
    AAGTACTTTT TTGGTCCAAA CAGTCCAGCC ACCAGAGATC AGCAGGAACA GGTAATGAAA
    CTAGCAGGAG GCTGGGGCAA GATTCTACAT GATCGACTGT CGGCGGCGGT CCTTATAGAA
    GTACAGAAAT ACGTCAGAAT GAGGATAGAG AGAAAGTGGC TTCCAATGTT TTTAGCAACA
    GAGGAGTACA GAGAGCGCCA GAGAGTGCAA GGACAAATGA AAGACGTTGC AGAAGATGTA
    ATATTTCAAA CAAACAAAAA GAAAATGGGA GTGTGGAAGC ATCTTGACAG TAAATGGGTC
    TCATCCTCCA AAGAGATAAT TGCCTTTCGT AAAGCCTTAA TGAATCCAGT AACTTCCAGT
    CAGTTTCAGC GCTTCATTTC ACTCAAAGGA GACTTTTTGG AGAATGGACT GCTTTTTTGG
    ATTGAAGTAC AGAAATACAA GGATCTCTGC CACTCCCACT GTGATGATTT CACAATTCAG
    AATAAAATTA CTGCTATCAT TAATTGCTTC ATCAACTCCT CCATCCCACC AGCCTTGCAG
    ATTGACATTC CCCAAGAGCA AGCAGAGAAA ATCTTAGAGC GCCGCCGGGA TTTAGGCCCA
    TATGTATTCC GTGAGGCTCA GATGACCGTC TTCAGCATCA TGTTCAAATT CTGGCCGGAG
    TTTTGTGATT TCCGTAGAAA TTTGACAGAA GAAAAGATTT TACCTTTTCT GGATAGAAAG
    AAATTAAAGC AGACAGAGGC AGTGAAAAGA AGAATGAGAG AAGAAGAGAG ACTTGCAAAG
    CAACAGCAAG AGGAGGCTAA GAGGAAAGGA ACATTATCCG ACTTGTATGG TGATAACGAA
    AGCACTTACA GCGGGGACAG GTTATCTGGA TACGGGGGAC ATGCACGGGA TGGATTTGCA
    CATGATGGAT ATGTGGCAGA TGGGTTTGGA CAAAGCAGGA ATGTATCTTG GAGTTATTCT
    AAGTATGTTG AAGCTTTGGA GAAGGAAAGG ATGCTTCTGA AAATTCAGGA AGATCTTGAA
    AAAAAGTCGC GGAGCTCCCT TCTGTCTGAT ATTTCGTCTC TTTACAGTGT AAGAACAGAA
    GCCTCAAAAA GAACCAACAG ATCTTCATCC GCACCCACTG ACAGGCCTTC CTCTGTTACA
    TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934464.1
    CDS376..4281
    Translation

    Target ORF information:

    RefSeq Version XM_002934418.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulator of G-protein signaling 22 (rgs22), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934418.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGTGCA AAAGGCTAGC AACTGTGCCC CCTGATATAA AAGAAGATGA TTTTGAGGAC 
    TTCTTAGCAA CAGATGACTT GCTTGTAGAT TACTTCAATG AATTTCTAAG CCTGCCGACC
    TTTCCAGAAC CAATCAAGTT CAACAAAACT TTTGGCATCT TCGAAGTTGC AAATAATGCA
    GGGGAACACC TAAAACAGCA GATAAAACAA GTGTTACAGG ACCTAGAATC TCCAAATCCC
    ATTTATGATG CTACCAGAAG AGTAACTGGC TTGAAACCTA GGGATCCTCC TCCTCCTGAC
    CTAAACATTG ATATTAATTA CAGTATTATG TGCTTGAATC GAGAGCAAGG GGTTCAGTGG
    ATAAAGAGAG AGAGACTACC GGCTTTCCTA AAAAGTGATT GCTATTTTGA GTACAGACTT
    GCTAAGCTGC TGGCACAGGT AAGAGCGAGC AACTCTGGAG TGACGGTTCT TGCTGACCCA
    AAGTACCAAC CATGGCAACT TAGGAAAGAG AGTGCTCCTT TGCCAAAAAC AGAAAACGAA
    GACCACAGCA TCATGCAGAA ATTCTTTGTC ACACTTGGCC AGGCTTCGTT TACTCAGACA
    AAGGAATGGT TTTCAGTAGC AAAGGACAGT GTTCAAACCA CTACAACTGA TTCATTAAAT
    GGCACAGTCG AGTGCAGAAC TGAAATTACC AGTACTAGAA TGAATTCACA GATTAGTAAA
    GCCTCAGAAG GTAGTCATAA TCAGGGAGTC CCTTGCAGGC CACTTTCTGC AAAATATCAT
    AATGGAAGTG AGATTGCTGT AGATGAAGAA GATGAGTATG CTGTATCTAT CACAGATGCA
    CCATGTCAAA CCCCTGCGAG GATTTATATT AATCAGGGGT CAGATAATTA TGAAGCACAA
    AACCAGAAAG CAAATGACTC TCAAGGCCTG CAGTCTGAAA ATTCACCAGT ATTTCCCACA
    GTGGAAGATT TTGCTCGTTC CTATGTTGAG CATGTTATGA AATCTGCAGT TTTAGAGCTA
    AGTGGGCAAC AAGTCCAGCA AAGTAACAAG GACCTTGATA TTCATGCTTT ATCTGCAGTG
    ACTATCCGTA GCTTGCAGAG TTCCGATACT TTATTTAGCT CCAAGAGCTC GGAAACGTCC
    ATTCAAGAGG AGATCATTAT TTCAGAGGCT GACAGTGATG AACTTGACAC CGCTGACTCC
    TTCTGTCTCA GAGACACTGA CTATCACATC AAGACCCGAC GGGAGTTTGA AATGTTTAAA
    TCATTTCTTA AAGGAACTTC AGGGGAGAAG TTATGGTGCC TCTGGATAGA CATTGAAAGG
    CTTCAAGCCA TAAAAGACCA CAAAAGGCAA CAGGGTCATC TGAATAAGAT GAAGAAACGC
    TATCTTGTGA CGAGCGGGGA CCTCTTTTTT ACAAGAGAAT TACTGTTTAA ACTGGAACTT
    TTGAATACAA ATCACTGGAA CAAAAATCAT TTGCAAAATG TGCAGAGTGA GGTGGTTAAA
    CCATTGCTTC TGTATTGGGG GCCTAGATTC TGTATGTCTG ATTCTGCTTT GGCTTATAAT
    GTTCACGCGG AGCTGAAAGC CTGGTCTGAT CGTCAGCTGA GACCCAAAAG AGATGCTGAC
    CCTTTTGCAA AGACGGTAAC GCTGTTACCA TTGAGACCCA AGTCTTGCGC CCCAAGAATT
    AGTTCATCAG TTCATCGTTT ACTTGAACAG AGCCCACCTC TAAAATCTTC AAAAAGAAGT
    AAGACGTGTT CTCCAAGTCG ACAATCCGGC AGCTTGATTT TTGCAAACAT AAAACCAAAA
    CAAACCAGCA GGAGTTTCCC TTGCAGTGCG GCTTATAATT CTCCATCTAG CATGAAACGA
    AAACATCAAA GAACAAACAG CTATCTGTCT GTGTCCCTGG GTTTGCCTAG AGAGAGTTTA
    AATATAGGAT CAGTGTATGA TCAAGATGTT CCCAAGGATG ATTTGAGTGA GGATGGTAGT
    ACCACCAGCG TATTAAGGGG CTCCAATATG GCAGAGATGT TAAATGCTCT GAATCTGGAC
    TCTCGAGCTG GCTATTTCTT TGCTACGTTC TGTGAGAAGT CAGGGAACAA GTTGTGGGAG
    AATGGGATCT ATTTCTGGTT TGATCTACAG TCCTATCATC ACAAGTTTTA CCAGGAGACA
    CTCCAACCAT TTCAGCTATG CAGACATTCC CAATTTCTGT TTGGCACGTA CCTTGCGCCT
    TCTGCTTCCA TGGATATCGG AGCCGAGCAG AACATTAAAA AAGAGATTTA CCAGAAACTT
    GATCCACCTT TTGAGGATTT ATTTGACTCG GCTGAAGAAT ACATTCTTAC ACTCCTGCTC
    TCTGCCTGGA GTCAGATGAC AGAGTCAGAC AGACAGGTCT ACAGCAAGGT GGAGCTGGTG
    GAGGAATCTC GGCAGCTGGA ATCACCACTC TACAAAAAGC TGCAAGCTTT GCAGCAGGAG
    AGAATGGCCA AGAAAAATCA AGATCAGATA TACAAATCCA TTTTCCTACC ACCTCCTGAC
    ATTGCCAAAG AGCCTGACCT CTGGGAACTG GTCCCTGAGG AATACAGGAA TTATAACCTC
    ACCACCTTAA TTCGACACCG GATGGAGCTT GAGCATTTCC GCAATTTTCT GGAAGATCAT
    TTTGCGAGCA TGGATCTCAT GTGCTGGATA GACCTGGAGC AGCTGAGGAG AATGCCACAC
    AAAGAAAAGG AGAAACGCCA GGAAAAATCC AAGGACATTA AAGACAAATA TTTAAACAAG
    AAGTACTTTT TTGGTCCAAA CAGTCCAGCC ACCAGAGATC AGCAGGAACA GGTAATGAAA
    CTAGCAGGAG GCTGGGGCAA GATTCTACAT GATCGACTGT CGGCGGCGGT CCTTATAGAA
    GTACAGAAAT ACGTCAGAAT GAGGATAGAG AGAAAGTGGC TTCCAATGTT TTTAGCAACA
    GAGGAGTACA GAGAGCGCCA GAGAGTGCAA GGACAAATGA AAGACGTTGC AGAAGATGTA
    ATATTTCAAA CAAACAAAAA GAAAATGGGA GTGTGGAAGC ATCTTGACAG TAAATGGGTC
    TCATCCTCCA AAGAGATAAT TGCCTTTCGT AAAGCCTTAA TGAATCCAGT AACTTCCAGT
    CAGTTTCAGC GCTTCATTTC ACTCAAAGGA GACTTTTTGG AGAATGGACT GCTTTTTTGG
    ATTGAAGTAC AGAAATACAA GGATCTCTGC CACTCCCACT GTGATGATTT CACAATTCAG
    AATAAAATTA CTGCTATCAT TAATTGCTTC ATCAACTCCT CCATCCCACC AGCCTTGCAG
    ATTGACATTC CCCAAGAGCA AGCAGAGAAA ATCTTAGAGC GCCGCCGGGA TTTAGGCCCA
    TATGTATTCC GTGAGGCTCA GATGACCGTC TTCAGCATCA TGTTCAAATT CTGGCCGGAG
    TTTTGTGATT TCCGTAGAAA TTTGACAGAA GAAAAGATTT TACCTTTTCT GGATAGAAAG
    AAATTAAAGC AGACAGAGGC AGTGAAAAGA AGAATGAGAG AAGAAGAGAG ACTTGCAAAG
    CAACAGCAAG AGGAGGCTAA GAGGAAAGGA ACATTATCCG ACTTGTATGG TGATAACGAA
    AGCACTTACA GCGGGGACAG GTTATCTGGA TACGGGGGAC ATGCACGGGA TGGATTTGCA
    CATGATGGAT ATGTGGCAGA TGGGTTTGGA CAAAGCAGGA ATGTATCTTG GAGTTATTCT
    AAGTATGTTG AAGCTTTGGA GAAGGAAAGG ATGCTTCTGA AAATTCAGGA AGATCTTGAA
    AAAAAGTCGC GGAGCTCCCT TCTGTCTGAT ATTTCGTCTC TTTACAGTGT AAGAACAGAA
    GCCTCAAAAA GAACCAACAG ATCTTCATCC GCACCCACTG ACAGGCCTTC CTCTGTTACA
    TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11471
    Clone ID Related Accession (Same CDS sequence) XM_018095160.1 , XM_018095160.2 , XM_002934418.5 , XM_002934418.4
    Accession Version XM_018095160.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulator of G-protein signaling 22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGTGCA AAAGGCTAGC AACTGTGCCC CCTGATATAA AAGAAGATGA TTTTGAGGAC 
    TTCTTAGCAA CAGATGACTT GCTTGTAGAT TACTTCAATG AATTTCTAAG CCTGCCGACC
    TTTCCAGAAC CAATCAAGTT CAACAAAACT TTTGGCATCT TCGAAGTTGC AAATAATGCA
    GGGGAACACC TAAAACAGCA GATAAAACAA GTGTTACAGG ACCTAGAATC TCCAAATCCC
    ATTTATGATG CTACCAGAAG AGTAACTGGC TTGAAACCTA GGGATCCTCC TCCTCCTGAC
    CTAAACATTG ATATTAATTA CAGTATTATG TGCTTGAATC GAGAGCAAGG GGTTCAGTGG
    ATAAAGAGAG AGAGACTACC GGCTTTCCTA AAAAGTGATT GCTATTTTGA GTACAGACTT
    GCTAAGCTGC TGGCACAGGT AAGAGCGAGC AACTCTGGAG TGACGGTTCT TGCTGACCCA
    AAGTACCAAC CATGGCAACT TAGGAAAGAG AGTGCTCCTT TGCCAAAAAC AGAAAACGAA
    GACCACAGCA TCATGCAGAA ATTCTTTGTC ACACTTGGCC AGGCTTCGTT TACTCAGACA
    AAGGAATGGT TTTCAGTAGC AAAGGACAGT GTTCAAACCA CTACAACTGA TTCATTAAAT
    GGCACAGTCG AGTGCAGAAC TGAAATTACC AGTACTAGAA TGAATTCACA GATTAGTAAA
    GCCTCAGAAG GTAGTCATAA TCAGGGAGTC CCTTGCAGGC CACTTTCTGC AAAATATCAT
    AATGGAAGTG AGATTGCTGT AGATGAAGAA GATGAGTATG CTGTATCTAT CACAGATGCA
    CCATGTCAAA CCCCTGCGAG GATTTATATT AATCAGGGGT CAGATAATTA TGAAGCACAA
    AACCAGAAAG CAAATGACTC TCAAGGCCTG CAGTCTGAAA ATTCACCAGT ATTTCCCACA
    GTGGAAGATT TTGCTCGTTC CTATGTTGAG CATGTTATGA AATCTGCAGT TTTAGAGCTA
    AGTGGGCAAC AAGTCCAGCA AAGTAACAAG GACCTTGATA TTCATGCTTT ATCTGCAGTG
    ACTATCCGTA GCTTGCAGAG TTCCGATACT TTATTTAGCT CCAAGAGCTC GGAAACGTCC
    ATTCAAGAGG AGATCATTAT TTCAGAGGCT GACAGTGATG AACTTGACAC CGCTGACTCC
    TTCTGTCTCA GAGACACTGA CTATCACATC AAGACCCGAC GGGAGTTTGA AATGTTTAAA
    TCATTTCTTA AAGGAACTTC AGGGGAGAAG TTATGGTGCC TCTGGATAGA CATTGAAAGG
    CTTCAAGCCA TAAAAGACCA CAAAAGGCAA CAGGGTCATC TGAATAAGAT GAAGAAACGC
    TATCTTGTGA CGAGCGGGGA CCTCTTTTTT ACAAGAGAAT TACTGTTTAA ACTGGAACTT
    TTGAATACAA ATCACTGGAA CAAAAATCAT TTGCAAAATG TGCAGAGTGA GGTGGTTAAA
    CCATTGCTTC TGTATTGGGG GCCTAGATTC TGTATGTCTG ATTCTGCTTT GGCTTATAAT
    GTTCACGCGG AGCTGAAAGC CTGGTCTGAT CGTCAGCTGA GACCCAAAAG AGATGCTGAC
    CCTTTTGCAA AGACGGTAAC GCTGTTACCA TTGAGACCCA AGTCTTGCGC CCCAAGAATT
    AGTTCATCAG TTCATCGTTT ACTTGAACAG AGCCCACCTC TAAAATCTTC AAAAAGAAGT
    AAGACGTGTT CTCCAAGTCG ACAATCCGGC AGCTTGATTT TTGCAAACAT AAAACCAAAA
    CAAACCAGCA GGAGTTTCCC TTGCAGTGCG GCTTATAATT CTCCATCTAG CATGAAACGA
    AAACATCAAA GAACAAACAG CTATCTGTCT GTGTCCCTGG GTTTGCCTAG AGAGAGTTTA
    AATATAGGAT CAGTGTATGA TCAAGATGTT CCCAAGGATG ATTTGAGTGA GGATGGTAGT
    ACCACCAGCG TATTAAGGGG CTCCAATATG GCAGAGATGT TAAATGCTCT GAATCTGGAC
    TCTCGAGCTG GCTATTTCTT TGCTACGTTC TGTGAGAAGT CAGGGAACAA GTTGTGGGAG
    AATGGGATCT ATTTCTGGTT TGATCTACAG TCCTATCATC ACAAGTTTTA CCAGGAGACA
    CTCCAACCAT TTCAGCTATG CAGACATTCC CAATTTCTGT TTGGCACGTA CCTTGCGCCT
    TCTGCTTCCA TGGATATCGG AGCCGAGCAG AACATTAAAA AAGAGATTTA CCAGAAACTT
    GATCCACCTT TTGAGGATTT ATTTGACTCG GCTGAAGAAT ACATTCTTAC ACTCCTGCTC
    TCTGCCTGGA GTCAGATGAC AGAGTCAGAC AGACAGGTCT ACAGCAAGGT GGAGCTGGTG
    GAGGAATCTC GGCAGCTGGA ATCACCACTC TACAAAAAGC TGCAAGCTTT GCAGCAGGAG
    AGAATGGCCA AGAAAAATCA AGATCAGATA TACAAATCCA TTTTCCTACC ACCTCCTGAC
    ATTGCCAAAG AGCCTGACCT CTGGGAACTG GTCCCTGAGG AATACAGGAA TTATAACCTC
    ACCACCTTAA TTCGACACCG GATGGAGCTT GAGCATTTCC GCAATTTTCT GGAAGATCAT
    TTTGCGAGCA TGGATCTCAT GTGCTGGATA GACCTGGAGC AGCTGAGGAG AATGCCACAC
    AAAGAAAAGG AGAAACGCCA GGAAAAATCC AAGGACATTA AAGACAAATA TTTAAACAAG
    AAGTACTTTT TTGGTCCAAA CAGTCCAGCC ACCAGAGATC AGCAGGAACA GGTAATGAAA
    CTAGCAGGAG GCTGGGGCAA GATTCTACAT GATCGACTGT CGGCGGCGGT CCTTATAGAA
    GTACAGAAAT ACGTCAGAAT GAGGATAGAG AGAAAGTGGC TTCCAATGTT TTTAGCAACA
    GAGGAGTACA GAGAGCGCCA GAGAGTGCAA GGACAAATGA AAGACGTTGC AGAAGATGTA
    ATATTTCAAA CAAACAAAAA GAAAATGGGA GTGTGGAAGC ATCTTGACAG TAAATGGGTC
    TCATCCTCCA AAGAGATAAT TGCCTTTCGT AAAGCCTTAA TGAATCCAGT AACTTCCAGT
    CAGTTTCAGC GCTTCATTTC ACTCAAAGGA GACTTTTTGG AGAATGGACT GCTTTTTTGG
    ATTGAAGTAC AGAAATACAA GGATCTCTGC CACTCCCACT GTGATGATTT CACAATTCAG
    AATAAAATTA CTGCTATCAT TAATTGCTTC ATCAACTCCT CCATCCCACC AGCCTTGCAG
    ATTGACATTC CCCAAGAGCA AGCAGAGAAA ATCTTAGAGC GCCGCCGGGA TTTAGGCCCA
    TATGTATTCC GTGAGGCTCA GATGACCGTC TTCAGCATCA TGTTCAAATT CTGGCCGGAG
    TTTTGTGATT TCCGTAGAAA TTTGACAGAA GAAAAGATTT TACCTTTTCT GGATAGAAAG
    AAATTAAAGC AGACAGAGGC AGTGAAAAGA AGAATGAGAG AAGAAGAGAG ACTTGCAAAG
    CAACAGCAAG AGGAGGCTAA GAGGAAAGGA ACATTATCCG ACTTGTATGG TGATAACGAA
    AGCACTTACA GCGGGGACAG GTTATCTGGA TACGGGGGAC ATGCACGGGA TGGATTTGCA
    CATGATGGAT ATGTGGCAGA TGGGTTTGGA CAAAGCAGGA ATGTATCTTG GAGTTATTCT
    AAGTATGTTG AAGCTTTGGA GAAGGAAAGG ATGCTTCTGA AAATTCAGGA AGATCTTGAA
    AAAAAGTCGC GGAGCTCCCT TCTGTCTGAT ATTTCGTCTC TTTACAGTGT AAGAACAGAA
    GCCTCAAAAA GAACCAACAG ATCTTCATCC GCACCCACTG ACAGGCCTTC CTCTGTTACA
    TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950649.1
    CDS279..4184
    Translation

    Target ORF information:

    RefSeq Version XM_018095160.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulator of G-protein signaling 22 (rgs22), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095160.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGTGCA AAAGGCTAGC AACTGTGCCC CCTGATATAA AAGAAGATGA TTTTGAGGAC 
    TTCTTAGCAA CAGATGACTT GCTTGTAGAT TACTTCAATG AATTTCTAAG CCTGCCGACC
    TTTCCAGAAC CAATCAAGTT CAACAAAACT TTTGGCATCT TCGAAGTTGC AAATAATGCA
    GGGGAACACC TAAAACAGCA GATAAAACAA GTGTTACAGG ACCTAGAATC TCCAAATCCC
    ATTTATGATG CTACCAGAAG AGTAACTGGC TTGAAACCTA GGGATCCTCC TCCTCCTGAC
    CTAAACATTG ATATTAATTA CAGTATTATG TGCTTGAATC GAGAGCAAGG GGTTCAGTGG
    ATAAAGAGAG AGAGACTACC GGCTTTCCTA AAAAGTGATT GCTATTTTGA GTACAGACTT
    GCTAAGCTGC TGGCACAGGT AAGAGCGAGC AACTCTGGAG TGACGGTTCT TGCTGACCCA
    AAGTACCAAC CATGGCAACT TAGGAAAGAG AGTGCTCCTT TGCCAAAAAC AGAAAACGAA
    GACCACAGCA TCATGCAGAA ATTCTTTGTC ACACTTGGCC AGGCTTCGTT TACTCAGACA
    AAGGAATGGT TTTCAGTAGC AAAGGACAGT GTTCAAACCA CTACAACTGA TTCATTAAAT
    GGCACAGTCG AGTGCAGAAC TGAAATTACC AGTACTAGAA TGAATTCACA GATTAGTAAA
    GCCTCAGAAG GTAGTCATAA TCAGGGAGTC CCTTGCAGGC CACTTTCTGC AAAATATCAT
    AATGGAAGTG AGATTGCTGT AGATGAAGAA GATGAGTATG CTGTATCTAT CACAGATGCA
    CCATGTCAAA CCCCTGCGAG GATTTATATT AATCAGGGGT CAGATAATTA TGAAGCACAA
    AACCAGAAAG CAAATGACTC TCAAGGCCTG CAGTCTGAAA ATTCACCAGT ATTTCCCACA
    GTGGAAGATT TTGCTCGTTC CTATGTTGAG CATGTTATGA AATCTGCAGT TTTAGAGCTA
    AGTGGGCAAC AAGTCCAGCA AAGTAACAAG GACCTTGATA TTCATGCTTT ATCTGCAGTG
    ACTATCCGTA GCTTGCAGAG TTCCGATACT TTATTTAGCT CCAAGAGCTC GGAAACGTCC
    ATTCAAGAGG AGATCATTAT TTCAGAGGCT GACAGTGATG AACTTGACAC CGCTGACTCC
    TTCTGTCTCA GAGACACTGA CTATCACATC AAGACCCGAC GGGAGTTTGA AATGTTTAAA
    TCATTTCTTA AAGGAACTTC AGGGGAGAAG TTATGGTGCC TCTGGATAGA CATTGAAAGG
    CTTCAAGCCA TAAAAGACCA CAAAAGGCAA CAGGGTCATC TGAATAAGAT GAAGAAACGC
    TATCTTGTGA CGAGCGGGGA CCTCTTTTTT ACAAGAGAAT TACTGTTTAA ACTGGAACTT
    TTGAATACAA ATCACTGGAA CAAAAATCAT TTGCAAAATG TGCAGAGTGA GGTGGTTAAA
    CCATTGCTTC TGTATTGGGG GCCTAGATTC TGTATGTCTG ATTCTGCTTT GGCTTATAAT
    GTTCACGCGG AGCTGAAAGC CTGGTCTGAT CGTCAGCTGA GACCCAAAAG AGATGCTGAC
    CCTTTTGCAA AGACGGTAAC GCTGTTACCA TTGAGACCCA AGTCTTGCGC CCCAAGAATT
    AGTTCATCAG TTCATCGTTT ACTTGAACAG AGCCCACCTC TAAAATCTTC AAAAAGAAGT
    AAGACGTGTT CTCCAAGTCG ACAATCCGGC AGCTTGATTT TTGCAAACAT AAAACCAAAA
    CAAACCAGCA GGAGTTTCCC TTGCAGTGCG GCTTATAATT CTCCATCTAG CATGAAACGA
    AAACATCAAA GAACAAACAG CTATCTGTCT GTGTCCCTGG GTTTGCCTAG AGAGAGTTTA
    AATATAGGAT CAGTGTATGA TCAAGATGTT CCCAAGGATG ATTTGAGTGA GGATGGTAGT
    ACCACCAGCG TATTAAGGGG CTCCAATATG GCAGAGATGT TAAATGCTCT GAATCTGGAC
    TCTCGAGCTG GCTATTTCTT TGCTACGTTC TGTGAGAAGT CAGGGAACAA GTTGTGGGAG
    AATGGGATCT ATTTCTGGTT TGATCTACAG TCCTATCATC ACAAGTTTTA CCAGGAGACA
    CTCCAACCAT TTCAGCTATG CAGACATTCC CAATTTCTGT TTGGCACGTA CCTTGCGCCT
    TCTGCTTCCA TGGATATCGG AGCCGAGCAG AACATTAAAA AAGAGATTTA CCAGAAACTT
    GATCCACCTT TTGAGGATTT ATTTGACTCG GCTGAAGAAT ACATTCTTAC ACTCCTGCTC
    TCTGCCTGGA GTCAGATGAC AGAGTCAGAC AGACAGGTCT ACAGCAAGGT GGAGCTGGTG
    GAGGAATCTC GGCAGCTGGA ATCACCACTC TACAAAAAGC TGCAAGCTTT GCAGCAGGAG
    AGAATGGCCA AGAAAAATCA AGATCAGATA TACAAATCCA TTTTCCTACC ACCTCCTGAC
    ATTGCCAAAG AGCCTGACCT CTGGGAACTG GTCCCTGAGG AATACAGGAA TTATAACCTC
    ACCACCTTAA TTCGACACCG GATGGAGCTT GAGCATTTCC GCAATTTTCT GGAAGATCAT
    TTTGCGAGCA TGGATCTCAT GTGCTGGATA GACCTGGAGC AGCTGAGGAG AATGCCACAC
    AAAGAAAAGG AGAAACGCCA GGAAAAATCC AAGGACATTA AAGACAAATA TTTAAACAAG
    AAGTACTTTT TTGGTCCAAA CAGTCCAGCC ACCAGAGATC AGCAGGAACA GGTAATGAAA
    CTAGCAGGAG GCTGGGGCAA GATTCTACAT GATCGACTGT CGGCGGCGGT CCTTATAGAA
    GTACAGAAAT ACGTCAGAAT GAGGATAGAG AGAAAGTGGC TTCCAATGTT TTTAGCAACA
    GAGGAGTACA GAGAGCGCCA GAGAGTGCAA GGACAAATGA AAGACGTTGC AGAAGATGTA
    ATATTTCAAA CAAACAAAAA GAAAATGGGA GTGTGGAAGC ATCTTGACAG TAAATGGGTC
    TCATCCTCCA AAGAGATAAT TGCCTTTCGT AAAGCCTTAA TGAATCCAGT AACTTCCAGT
    CAGTTTCAGC GCTTCATTTC ACTCAAAGGA GACTTTTTGG AGAATGGACT GCTTTTTTGG
    ATTGAAGTAC AGAAATACAA GGATCTCTGC CACTCCCACT GTGATGATTT CACAATTCAG
    AATAAAATTA CTGCTATCAT TAATTGCTTC ATCAACTCCT CCATCCCACC AGCCTTGCAG
    ATTGACATTC CCCAAGAGCA AGCAGAGAAA ATCTTAGAGC GCCGCCGGGA TTTAGGCCCA
    TATGTATTCC GTGAGGCTCA GATGACCGTC TTCAGCATCA TGTTCAAATT CTGGCCGGAG
    TTTTGTGATT TCCGTAGAAA TTTGACAGAA GAAAAGATTT TACCTTTTCT GGATAGAAAG
    AAATTAAAGC AGACAGAGGC AGTGAAAAGA AGAATGAGAG AAGAAGAGAG ACTTGCAAAG
    CAACAGCAAG AGGAGGCTAA GAGGAAAGGA ACATTATCCG ACTTGTATGG TGATAACGAA
    AGCACTTACA GCGGGGACAG GTTATCTGGA TACGGGGGAC ATGCACGGGA TGGATTTGCA
    CATGATGGAT ATGTGGCAGA TGGGTTTGGA CAAAGCAGGA ATGTATCTTG GAGTTATTCT
    AAGTATGTTG AAGCTTTGGA GAAGGAAAGG ATGCTTCTGA AAATTCAGGA AGATCTTGAA
    AAAAAGTCGC GGAGCTCCCT TCTGTCTGAT ATTTCGTCTC TTTACAGTGT AAGAACAGAA
    GCCTCAAAAA GAACCAACAG ATCTTCATCC GCACCCACTG ACAGGCCTTC CTCTGTTACA
    TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11471
    Clone ID Related Accession (Same CDS sequence) XM_018095160.1 , XM_018095160.2 , XM_002934418.5 , XM_002934418.4
    Accession Version XM_002934418.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product regulator of G-protein signaling 22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934418.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGTGCA AAAGGCTAGC AACTGTGCCC CCTGATATAA AAGAAGATGA TTTTGAGGAC 
    TTCTTAGCAA CAGATGACTT GCTTGTAGAT TACTTCAATG AATTTCTAAG CCTGCCGACC
    TTTCCAGAAC CAATCAAGTT CAACAAAACT TTTGGCATCT TCGAAGTTGC AAATAATGCA
    GGGGAACACC TAAAACAGCA GATAAAACAA GTGTTACAGG ACCTAGAATC TCCAAATCCC
    ATTTATGATG CTACCAGAAG AGTAACTGGC TTGAAACCTA GGGATCCTCC TCCTCCTGAC
    CTAAACATTG ATATTAATTA CAGTATTATG TGCTTGAATC GAGAGCAAGG GGTTCAGTGG
    ATAAAGAGAG AGAGACTACC GGCTTTCCTA AAAAGTGATT GCTATTTTGA GTACAGACTT
    GCTAAGCTGC TGGCACAGGT AAGAGCGAGC AACTCTGGAG TGACGGTTCT TGCTGACCCA
    AAGTACCAAC CATGGCAACT TAGGAAAGAG AGTGCTCCTT TGCCAAAAAC AGAAAACGAA
    GACCACAGCA TCATGCAGAA ATTCTTTGTC ACACTTGGCC AGGCTTCGTT TACTCAGACA
    AAGGAATGGT TTTCAGTAGC AAAGGACAGT GTTCAAACCA CTACAACTGA TTCATTAAAT
    GGCACAGTCG AGTGCAGAAC TGAAATTACC AGTACTAGAA TGAATTCACA GATTAGTAAA
    GCCTCAGAAG GTAGTCATAA TCAGGGAGTC CCTTGCAGGC CACTTTCTGC AAAATATCAT
    AATGGAAGTG AGATTGCTGT AGATGAAGAA GATGAGTATG CTGTATCTAT CACAGATGCA
    CCATGTCAAA CCCCTGCGAG GATTTATATT AATCAGGGGT CAGATAATTA TGAAGCACAA
    AACCAGAAAG CAAATGACTC TCAAGGCCTG CAGTCTGAAA ATTCACCAGT ATTTCCCACA
    GTGGAAGATT TTGCTCGTTC CTATGTTGAG CATGTTATGA AATCTGCAGT TTTAGAGCTA
    AGTGGGCAAC AAGTCCAGCA AAGTAACAAG GACCTTGATA TTCATGCTTT ATCTGCAGTG
    ACTATCCGTA GCTTGCAGAG TTCCGATACT TTATTTAGCT CCAAGAGCTC GGAAACGTCC
    ATTCAAGAGG AGATCATTAT TTCAGAGGCT GACAGTGATG AACTTGACAC CGCTGACTCC
    TTCTGTCTCA GAGACACTGA CTATCACATC AAGACCCGAC GGGAGTTTGA AATGTTTAAA
    TCATTTCTTA AAGGAACTTC AGGGGAGAAG TTATGGTGCC TCTGGATAGA CATTGAAAGG
    CTTCAAGCCA TAAAAGACCA CAAAAGGCAA CAGGGTCATC TGAATAAGAT GAAGAAACGC
    TATCTTGTGA CGAGCGGGGA CCTCTTTTTT ACAAGAGAAT TACTGTTTAA ACTGGAACTT
    TTGAATACAA ATCACTGGAA CAAAAATCAT TTGCAAAATG TGCAGAGTGA GGTGGTTAAA
    CCATTGCTTC TGTATTGGGG GCCTAGATTC TGTATGTCTG ATTCTGCTTT GGCTTATAAT
    GTTCACGCGG AGCTGAAAGC CTGGTCTGAT CGTCAGCTGA GACCCAAAAG AGATGCTGAC
    CCTTTTGCAA AGACGGTAAC GCTGTTACCA TTGAGACCCA AGTCTTGCGC CCCAAGAATT
    AGTTCATCAG TTCATCGTTT ACTTGAACAG AGCCCACCTC TAAAATCTTC AAAAAGAAGT
    AAGACGTGTT CTCCAAGTCG ACAATCCGGC AGCTTGATTT TTGCAAACAT AAAACCAAAA
    CAAACCAGCA GGAGTTTCCC TTGCAGTGCG GCTTATAATT CTCCATCTAG CATGAAACGA
    AAACATCAAA GAACAAACAG CTATCTGTCT GTGTCCCTGG GTTTGCCTAG AGAGAGTTTA
    AATATAGGAT CAGTGTATGA TCAAGATGTT CCCAAGGATG ATTTGAGTGA GGATGGTAGT
    ACCACCAGCG TATTAAGGGG CTCCAATATG GCAGAGATGT TAAATGCTCT GAATCTGGAC
    TCTCGAGCTG GCTATTTCTT TGCTACGTTC TGTGAGAAGT CAGGGAACAA GTTGTGGGAG
    AATGGGATCT ATTTCTGGTT TGATCTACAG TCCTATCATC ACAAGTTTTA CCAGGAGACA
    CTCCAACCAT TTCAGCTATG CAGACATTCC CAATTTCTGT TTGGCACGTA CCTTGCGCCT
    TCTGCTTCCA TGGATATCGG AGCCGAGCAG AACATTAAAA AAGAGATTTA CCAGAAACTT
    GATCCACCTT TTGAGGATTT ATTTGACTCG GCTGAAGAAT ACATTCTTAC ACTCCTGCTC
    TCTGCCTGGA GTCAGATGAC AGAGTCAGAC AGACAGGTCT ACAGCAAGGT GGAGCTGGTG
    GAGGAATCTC GGCAGCTGGA ATCACCACTC TACAAAAAGC TGCAAGCTTT GCAGCAGGAG
    AGAATGGCCA AGAAAAATCA AGATCAGATA TACAAATCCA TTTTCCTACC ACCTCCTGAC
    ATTGCCAAAG AGCCTGACCT CTGGGAACTG GTCCCTGAGG AATACAGGAA TTATAACCTC
    ACCACCTTAA TTCGACACCG GATGGAGCTT GAGCATTTCC GCAATTTTCT GGAAGATCAT
    TTTGCGAGCA TGGATCTCAT GTGCTGGATA GACCTGGAGC AGCTGAGGAG AATGCCACAC
    AAAGAAAAGG AGAAACGCCA GGAAAAATCC AAGGACATTA AAGACAAATA TTTAAACAAG
    AAGTACTTTT TTGGTCCAAA CAGTCCAGCC ACCAGAGATC AGCAGGAACA GGTAATGAAA
    CTAGCAGGAG GCTGGGGCAA GATTCTACAT GATCGACTGT CGGCGGCGGT CCTTATAGAA
    GTACAGAAAT ACGTCAGAAT GAGGATAGAG AGAAAGTGGC TTCCAATGTT TTTAGCAACA
    GAGGAGTACA GAGAGCGCCA GAGAGTGCAA GGACAAATGA AAGACGTTGC AGAAGATGTA
    ATATTTCAAA CAAACAAAAA GAAAATGGGA GTGTGGAAGC ATCTTGACAG TAAATGGGTC
    TCATCCTCCA AAGAGATAAT TGCCTTTCGT AAAGCCTTAA TGAATCCAGT AACTTCCAGT
    CAGTTTCAGC GCTTCATTTC ACTCAAAGGA GACTTTTTGG AGAATGGACT GCTTTTTTGG
    ATTGAAGTAC AGAAATACAA GGATCTCTGC CACTCCCACT GTGATGATTT CACAATTCAG
    AATAAAATTA CTGCTATCAT TAATTGCTTC ATCAACTCCT CCATCCCACC AGCCTTGCAG
    ATTGACATTC CCCAAGAGCA AGCAGAGAAA ATCTTAGAGC GCCGCCGGGA TTTAGGCCCA
    TATGTATTCC GTGAGGCTCA GATGACCGTC TTCAGCATCA TGTTCAAATT CTGGCCGGAG
    TTTTGTGATT TCCGTAGAAA TTTGACAGAA GAAAAGATTT TACCTTTTCT GGATAGAAAG
    AAATTAAAGC AGACAGAGGC AGTGAAAAGA AGAATGAGAG AAGAAGAGAG ACTTGCAAAG
    CAACAGCAAG AGGAGGCTAA GAGGAAAGGA ACATTATCCG ACTTGTATGG TGATAACGAA
    AGCACTTACA GCGGGGACAG GTTATCTGGA TACGGGGGAC ATGCACGGGA TGGATTTGCA
    CATGATGGAT ATGTGGCAGA TGGGTTTGGA CAAAGCAGGA ATGTATCTTG GAGTTATTCT
    AAGTATGTTG AAGCTTTGGA GAAGGAAAGG ATGCTTCTGA AAATTCAGGA AGATCTTGAA
    AAAAAGTCGC GGAGCTCCCT TCTGTCTGAT ATTTCGTCTC TTTACAGTGT AAGAACAGAA
    GCCTCAAAAA GAACCAACAG ATCTTCATCC GCACCCACTG ACAGGCCTTC CTCTGTTACA
    TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002934464.1
    CDS279..4184
    Translation

    Target ORF information:

    RefSeq Version XM_002934418.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis regulator of G-protein signaling 22 (rgs22), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002934418.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGTGCA AAAGGCTAGC AACTGTGCCC CCTGATATAA AAGAAGATGA TTTTGAGGAC 
    TTCTTAGCAA CAGATGACTT GCTTGTAGAT TACTTCAATG AATTTCTAAG CCTGCCGACC
    TTTCCAGAAC CAATCAAGTT CAACAAAACT TTTGGCATCT TCGAAGTTGC AAATAATGCA
    GGGGAACACC TAAAACAGCA GATAAAACAA GTGTTACAGG ACCTAGAATC TCCAAATCCC
    ATTTATGATG CTACCAGAAG AGTAACTGGC TTGAAACCTA GGGATCCTCC TCCTCCTGAC
    CTAAACATTG ATATTAATTA CAGTATTATG TGCTTGAATC GAGAGCAAGG GGTTCAGTGG
    ATAAAGAGAG AGAGACTACC GGCTTTCCTA AAAAGTGATT GCTATTTTGA GTACAGACTT
    GCTAAGCTGC TGGCACAGGT AAGAGCGAGC AACTCTGGAG TGACGGTTCT TGCTGACCCA
    AAGTACCAAC CATGGCAACT TAGGAAAGAG AGTGCTCCTT TGCCAAAAAC AGAAAACGAA
    GACCACAGCA TCATGCAGAA ATTCTTTGTC ACACTTGGCC AGGCTTCGTT TACTCAGACA
    AAGGAATGGT TTTCAGTAGC AAAGGACAGT GTTCAAACCA CTACAACTGA TTCATTAAAT
    GGCACAGTCG AGTGCAGAAC TGAAATTACC AGTACTAGAA TGAATTCACA GATTAGTAAA
    GCCTCAGAAG GTAGTCATAA TCAGGGAGTC CCTTGCAGGC CACTTTCTGC AAAATATCAT
    AATGGAAGTG AGATTGCTGT AGATGAAGAA GATGAGTATG CTGTATCTAT CACAGATGCA
    CCATGTCAAA CCCCTGCGAG GATTTATATT AATCAGGGGT CAGATAATTA TGAAGCACAA
    AACCAGAAAG CAAATGACTC TCAAGGCCTG CAGTCTGAAA ATTCACCAGT ATTTCCCACA
    GTGGAAGATT TTGCTCGTTC CTATGTTGAG CATGTTATGA AATCTGCAGT TTTAGAGCTA
    AGTGGGCAAC AAGTCCAGCA AAGTAACAAG GACCTTGATA TTCATGCTTT ATCTGCAGTG
    ACTATCCGTA GCTTGCAGAG TTCCGATACT TTATTTAGCT CCAAGAGCTC GGAAACGTCC
    ATTCAAGAGG AGATCATTAT TTCAGAGGCT GACAGTGATG AACTTGACAC CGCTGACTCC
    TTCTGTCTCA GAGACACTGA CTATCACATC AAGACCCGAC GGGAGTTTGA AATGTTTAAA
    TCATTTCTTA AAGGAACTTC AGGGGAGAAG TTATGGTGCC TCTGGATAGA CATTGAAAGG
    CTTCAAGCCA TAAAAGACCA CAAAAGGCAA CAGGGTCATC TGAATAAGAT GAAGAAACGC
    TATCTTGTGA CGAGCGGGGA CCTCTTTTTT ACAAGAGAAT TACTGTTTAA ACTGGAACTT
    TTGAATACAA ATCACTGGAA CAAAAATCAT TTGCAAAATG TGCAGAGTGA GGTGGTTAAA
    CCATTGCTTC TGTATTGGGG GCCTAGATTC TGTATGTCTG ATTCTGCTTT GGCTTATAAT
    GTTCACGCGG AGCTGAAAGC CTGGTCTGAT CGTCAGCTGA GACCCAAAAG AGATGCTGAC
    CCTTTTGCAA AGACGGTAAC GCTGTTACCA TTGAGACCCA AGTCTTGCGC CCCAAGAATT
    AGTTCATCAG TTCATCGTTT ACTTGAACAG AGCCCACCTC TAAAATCTTC AAAAAGAAGT
    AAGACGTGTT CTCCAAGTCG ACAATCCGGC AGCTTGATTT TTGCAAACAT AAAACCAAAA
    CAAACCAGCA GGAGTTTCCC TTGCAGTGCG GCTTATAATT CTCCATCTAG CATGAAACGA
    AAACATCAAA GAACAAACAG CTATCTGTCT GTGTCCCTGG GTTTGCCTAG AGAGAGTTTA
    AATATAGGAT CAGTGTATGA TCAAGATGTT CCCAAGGATG ATTTGAGTGA GGATGGTAGT
    ACCACCAGCG TATTAAGGGG CTCCAATATG GCAGAGATGT TAAATGCTCT GAATCTGGAC
    TCTCGAGCTG GCTATTTCTT TGCTACGTTC TGTGAGAAGT CAGGGAACAA GTTGTGGGAG
    AATGGGATCT ATTTCTGGTT TGATCTACAG TCCTATCATC ACAAGTTTTA CCAGGAGACA
    CTCCAACCAT TTCAGCTATG CAGACATTCC CAATTTCTGT TTGGCACGTA CCTTGCGCCT
    TCTGCTTCCA TGGATATCGG AGCCGAGCAG AACATTAAAA AAGAGATTTA CCAGAAACTT
    GATCCACCTT TTGAGGATTT ATTTGACTCG GCTGAAGAAT ACATTCTTAC ACTCCTGCTC
    TCTGCCTGGA GTCAGATGAC AGAGTCAGAC AGACAGGTCT ACAGCAAGGT GGAGCTGGTG
    GAGGAATCTC GGCAGCTGGA ATCACCACTC TACAAAAAGC TGCAAGCTTT GCAGCAGGAG
    AGAATGGCCA AGAAAAATCA AGATCAGATA TACAAATCCA TTTTCCTACC ACCTCCTGAC
    ATTGCCAAAG AGCCTGACCT CTGGGAACTG GTCCCTGAGG AATACAGGAA TTATAACCTC
    ACCACCTTAA TTCGACACCG GATGGAGCTT GAGCATTTCC GCAATTTTCT GGAAGATCAT
    TTTGCGAGCA TGGATCTCAT GTGCTGGATA GACCTGGAGC AGCTGAGGAG AATGCCACAC
    AAAGAAAAGG AGAAACGCCA GGAAAAATCC AAGGACATTA AAGACAAATA TTTAAACAAG
    AAGTACTTTT TTGGTCCAAA CAGTCCAGCC ACCAGAGATC AGCAGGAACA GGTAATGAAA
    CTAGCAGGAG GCTGGGGCAA GATTCTACAT GATCGACTGT CGGCGGCGGT CCTTATAGAA
    GTACAGAAAT ACGTCAGAAT GAGGATAGAG AGAAAGTGGC TTCCAATGTT TTTAGCAACA
    GAGGAGTACA GAGAGCGCCA GAGAGTGCAA GGACAAATGA AAGACGTTGC AGAAGATGTA
    ATATTTCAAA CAAACAAAAA GAAAATGGGA GTGTGGAAGC ATCTTGACAG TAAATGGGTC
    TCATCCTCCA AAGAGATAAT TGCCTTTCGT AAAGCCTTAA TGAATCCAGT AACTTCCAGT
    CAGTTTCAGC GCTTCATTTC ACTCAAAGGA GACTTTTTGG AGAATGGACT GCTTTTTTGG
    ATTGAAGTAC AGAAATACAA GGATCTCTGC CACTCCCACT GTGATGATTT CACAATTCAG
    AATAAAATTA CTGCTATCAT TAATTGCTTC ATCAACTCCT CCATCCCACC AGCCTTGCAG
    ATTGACATTC CCCAAGAGCA AGCAGAGAAA ATCTTAGAGC GCCGCCGGGA TTTAGGCCCA
    TATGTATTCC GTGAGGCTCA GATGACCGTC TTCAGCATCA TGTTCAAATT CTGGCCGGAG
    TTTTGTGATT TCCGTAGAAA TTTGACAGAA GAAAAGATTT TACCTTTTCT GGATAGAAAG
    AAATTAAAGC AGACAGAGGC AGTGAAAAGA AGAATGAGAG AAGAAGAGAG ACTTGCAAAG
    CAACAGCAAG AGGAGGCTAA GAGGAAAGGA ACATTATCCG ACTTGTATGG TGATAACGAA
    AGCACTTACA GCGGGGACAG GTTATCTGGA TACGGGGGAC ATGCACGGGA TGGATTTGCA
    CATGATGGAT ATGTGGCAGA TGGGTTTGGA CAAAGCAGGA ATGTATCTTG GAGTTATTCT
    AAGTATGTTG AAGCTTTGGA GAAGGAAAGG ATGCTTCTGA AAATTCAGGA AGATCTTGAA
    AAAAAGTCGC GGAGCTCCCT TCTGTCTGAT ATTTCGTCTC TTTACAGTGT AAGAACAGAA
    GCCTCAAAAA GAACCAACAG ATCTTCATCC GCACCCACTG ACAGGCCTTC CTCTGTTACA
    TACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.