spire1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	spire1
Entrez Gene ID	100492330
Full Name	spire-type actin nucleation factor 1
Synonyms	pEg6,spire
General protein information	Preferred Names spire-type actin nucleation factor 1 Names protein spire homolog 1 spire homolog 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031903692.1	XP_031759552.1	protein spire homolog 1 isoform X3
XM_031903693.1	XP_031759553.1	protein spire homolog 1 isoform X4
XM_031903690.1	XP_031759550.1	protein spire homolog 1 isoform X1
XM_031903691.1	XP_031759551.1	protein spire homolog 1 isoform X2
XM_012965027.2	XP_012820481.1	protein spire homolog 1
XM_031903694.1	XP_031759554.1	protein spire homolog 1 isoform X5

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPIRE1	XP_547684.4
Gallus gallus (chicken)	SPIRE1	XP_004939777.1
Mus musculus (house mouse)	Spire1	NP_919336.1
Xenopus tropicalis (tropical clawed frog)	spire1	XP_002936125.2
Pan troglodytes (chimpanzee)	SPIRE1	XP_003315842.1
Rattus norvegicus (Norway rat)	Spire1	NP_001100851.1
Danio rerio (zebrafish)	spire1a	NP_001038312.2
Homo sapiens (human)	SPIRE1	NP_001122098.1
Bos taurus (cattle)	SPIRE1	XP_005224371.1

Gene Ontology

The following spire1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spire1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa35360	XM_012965027.2 Latest version!	Xenopus tropicalis spire type actin nucleation factor 1 (spire1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa60054	XM_031903690.1 Latest version!	Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa60055	XM_031903691.1 Latest version!	Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa60056	XM_031903692.1 Latest version!	Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa60057	XM_031903693.1 Latest version!	Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa60058	XM_031903694.1 Latest version!	Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spire1 ( XM_012965027.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012820481.1 Xenopus tropicalis spire type actin nucleation factor 1 (spire1), mRNA.

Price & Availability↑

CloneID	OXa35360
Clone ID Related Accession (Same CDS sequence)	XM_012965027.2
Accession Version	XM_012965027.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein spire homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965027.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCCGAGT CTCAAGCGGC GCCTCGGAAA CGTAGTGAGG CTCAGGGCAG CGGGACTGGC 
GCGGCGGGAG ATGGGGAGTG TTTCTCGCTG GAGGAGATAT TGGATCTATA CAAGCAGCCG 
ATCAATGAGG AGCAAGCTTG GGCTGTGTGC TACCAGTGCT GCGGGGCCCT GCTGGAGGGG 
AGGGGGGGTG GCATTAAGGG AAGGAAGCTA ACTGGAGCCG CCGAGATTTG TATTCGTAAG 
GATGGAGCTG TCATCACCAG GCAGGAGTCG GCGGCAGCAG AGTCGATTGA TTGCAGGTTT 
ACAGAAACAG AGGTTATTGT GTCTCTTGGG ATCATCATTT ACAAAGCGTT GGATTATGGC 
TTGAAGGAGA ATGAGGAAAG AGAGCTTAGC CCTCCTTTGG AGCTACTGAT TGACAACATG 
ACTAACACAG ACATAGCTGG AAATAATGAT GAAGGCTATG ATGCACTGGA TGAAGATGAG 
ACCGACAGTG AAAGAGAATC AGCAGGATTC CGACACTCTT ACAGAAATGT AATGAAGCTT 
TGTACTACTC ATTTGCCAAC CCCATCAGAA GCACCCAATC ACTACCAAGC AGTATGCCGG 
GCCTTGTATG CAGAGACTAT GGAGTTACAC ATGTTCCTGA CCAAAATTAA AAGTGCAAAA 
GAGAGTTTAA AGAAGATTCA AGAAATGGAA AGAGATACAA CAGACAGAGC TTGCTCTGAT 
TTAGATGAGC TGAAAAACGC AGACTGGGCT CGGTTTTGGG TGCAGGTCAT GCGGGATCTA 
AGGAACGGTG TCAAGCTAAA GAAAGTGCAA GAGCGGCAGT ACGACCTGTT ACCAATAGAG 
TTTGAGCTGA CACCATATGA GATGCTTATG GATGATATTC GATCTAAACG ATACACCTTA 
AGGAAAGTCA TGGTTCAGGG TGACATCCCA CCCCGATTAA AGAAAAGTGC TCATGAAATA 
ATCCTGGATT TTATACGATC TAGACCTCCA CTTAATCCTG TAGCTGCTCG AAAGCTGAAA 
CCCACCCCAC CCCGTCCACG AAGCCTCCAT GAACAGATCC TGGCAGAGAT CAAGTCAGAG 
CGAAAGCTGC GTCCTGTTTC CCCTGATGAG ATTAGGCGCA GCAGATTAGA TGTATCTACT 
CCTGAAGCAA CAAGAAAAGT GTCTGACAAA ACAGTTTTAA ATGGTGGGGT GACGCCTAAA 
GCCAAGGTCA ATGGGCACCC GATGCAGGTG GCAGTACAGA GGAAGAAGCT CCTGAAGGCT 
CCAGCCTTGG CCGAGTTTGA GAGTTCAGAA TCAGAGGAGG ACACTGTGCA CAAATCAACG 
AGCAGCAGTA GTGTGTCTAC TTCCCAGCCA GATGATGTCT CCCCGGAATC TGCATCCAGC 
AGGAAATGTG GCTGGTTGAT GGATGCTGAT CTTTCAGGGC CCATGGGCAG TATGTTTGTA 
TTCATGGAAG CTATTGACTT ACAGGAGGAG TTCTGCTACC CGGTGGAATG CCTGGCCCTC 
ACAGTGGAGG AGGTCATGCA CATACGGCAG GTGCTGGTTA AAGCTGAGTT GGAGAAGTTC 
CAGCAGTATA AAGATATCTA TAATGCCCTG AAGAAAGGAA AGCTTTGTTT GTGCTGTCGA 
ACAAAAAGGT TTTCATTATT TACATGGTCA TACACTTGCC AGTTTTGCAA AAGACCAGTG 
TGCTCACAAT GTTGCAAAAA GATGAGATTA CCTTCTAAGC CATATTCAAC ACTTCCCATC 
TTCTCCCTGG GTCCTTCAAC CTTGCAGCGA GGGGACAGTT TCCTAAAGCC AGAGAAGCCA 
TCCACCAGTC ACCAGCGGCC ACTGAGAAGC TTCCCCAAGA TTCCTTCCAA GTCTAAGTCA 
GTGGAGAGAC CAGATGAGGA ATTTCAGTTT CCTAAAGAGC TCATGGAAGA CTGGAGCACC 
ATGGAGGTGT GTGTGGACTG TAAAAAGTTT ATCTCGGAAA TTATCAGTTC TAGTCGGCGC 
AGTTTAACAC TCGCTAACAA GAGGGCTCGC TTAAAACGGA AGACGCAATC GTTCTACTTG 
TCCTCGACAG CAGCCTCGGA GTTTCGGCCC ACTGAGAGAA CAATCAATGA GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012820481.1
CDS	208..2304
Translation	MAESQAAPRKRSEAQGSGTGAAGDGECFSLEEILDLYKQPINEEQAWAVCYQCCGALLEGRGGGIKGRKLTGAAEICIRKDGAVITRQESAAAESIDCRFTETEVIVSLGIIIYKALDYGLKENEERELSPPLELLIDNMTNTDIAGNNDEGYDALDEDETDSERESAGFRHSYRNVMKLCTTHLPTPSEAPNHYQAVCRALYAETMELHMFLTKIKSAKESLKKIQEMERDTTDRACSDLDELKNADWARFWVQVMRDLRNGVKLKKVQERQYDLLPIEFELTPYEMLMDDIRSKRYTLRKVMVQGDIPPRLKKSAHEIILDFIRSRPPLNPVAARKLKPTPPRPRSLHEQILAEIKSERKLRPVSPDEIRRSRLDVSTPEATRKVSDKTVLNGGVTPKAKVNGHPMQVAVQRKKLLKAPALAEFESSESEEDTVHKSTSSSSVSTSQPDDVSPESASSRKCGWLMDADLSGPMGSMFVFMEAIDLQEEFCYPVECLALTVEEVMHIRQVLVKAELEKFQQYKDIYNALKKGKLCLCCRTKRFSLFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSTLQRGDSFLKPEKPSTSHQRPLRSFPKIPSKSKSVERPDEEFQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLTLANKRARLKRKTQSFYLSSTAASEFRPTERTINEI

Target ORF information:

RefSeq Version	XM_012965027.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spire type actin nucleation factor 1 (spire1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012965027.2

ORF Insert Sequence:

ATGGCCGAGT CTCAAGCGGC GCCTCGGAAA CGTAGTGAGG CTCAGGGCAG CGGGACTGGC 
GCGGCGGGAG ATGGGGAGTG TTTCTCGCTG GAGGAGATAT TGGATCTATA CAAGCAGCCG 
ATCAATGAGG AGCAAGCTTG GGCTGTGTGC TACCAGTGCT GCGGGGCCCT GCTGGAGGGG 
AGGGGGGGTG GCATTAAGGG AAGGAAGCTA ACTGGAGCCG CCGAGATTTG TATTCGTAAG 
GATGGAGCTG TCATCACCAG GCAGGAGTCG GCGGCAGCAG AGTCGATTGA TTGCAGGTTT 
ACAGAAACAG AGGTTATTGT GTCTCTTGGG ATCATCATTT ACAAAGCGTT GGATTATGGC 
TTGAAGGAGA ATGAGGAAAG AGAGCTTAGC CCTCCTTTGG AGCTACTGAT TGACAACATG 
ACTAACACAG ACATAGCTGG AAATAATGAT GAAGGCTATG ATGCACTGGA TGAAGATGAG 
ACCGACAGTG AAAGAGAATC AGCAGGATTC CGACACTCTT ACAGAAATGT AATGAAGCTT 
TGTACTACTC ATTTGCCAAC CCCATCAGAA GCACCCAATC ACTACCAAGC AGTATGCCGG 
GCCTTGTATG CAGAGACTAT GGAGTTACAC ATGTTCCTGA CCAAAATTAA AAGTGCAAAA 
GAGAGTTTAA AGAAGATTCA AGAAATGGAA AGAGATACAA CAGACAGAGC TTGCTCTGAT 
TTAGATGAGC TGAAAAACGC AGACTGGGCT CGGTTTTGGG TGCAGGTCAT GCGGGATCTA 
AGGAACGGTG TCAAGCTAAA GAAAGTGCAA GAGCGGCAGT ACGACCTGTT ACCAATAGAG 
TTTGAGCTGA CACCATATGA GATGCTTATG GATGATATTC GATCTAAACG ATACACCTTA 
AGGAAAGTCA TGGTTCAGGG TGACATCCCA CCCCGATTAA AGAAAAGTGC TCATGAAATA 
ATCCTGGATT TTATACGATC TAGACCTCCA CTTAATCCTG TAGCTGCTCG AAAGCTGAAA 
CCCACCCCAC CCCGTCCACG AAGCCTCCAT GAACAGATCC TGGCAGAGAT CAAGTCAGAG 
CGAAAGCTGC GTCCTGTTTC CCCTGATGAG ATTAGGCGCA GCAGATTAGA TGTATCTACT 
CCTGAAGCAA CAAGAAAAGT GTCTGACAAA ACAGTTTTAA ATGGTGGGGT GACGCCTAAA 
GCCAAGGTCA ATGGGCACCC GATGCAGGTG GCAGTACAGA GGAAGAAGCT CCTGAAGGCT 
CCAGCCTTGG CCGAGTTTGA GAGTTCAGAA TCAGAGGAGG ACACTGTGCA CAAATCAACG 
AGCAGCAGTA GTGTGTCTAC TTCCCAGCCA GATGATGTCT CCCCGGAATC TGCATCCAGC 
AGGAAATGTG GCTGGTTGAT GGATGCTGAT CTTTCAGGGC CCATGGGCAG TATGTTTGTA 
TTCATGGAAG CTATTGACTT ACAGGAGGAG TTCTGCTACC CGGTGGAATG CCTGGCCCTC 
ACAGTGGAGG AGGTCATGCA CATACGGCAG GTGCTGGTTA AAGCTGAGTT GGAGAAGTTC 
CAGCAGTATA AAGATATCTA TAATGCCCTG AAGAAAGGAA AGCTTTGTTT GTGCTGTCGA 
ACAAAAAGGT TTTCATTATT TACATGGTCA TACACTTGCC AGTTTTGCAA AAGACCAGTG 
TGCTCACAAT GTTGCAAAAA GATGAGATTA CCTTCTAAGC CATATTCAAC ACTTCCCATC 
TTCTCCCTGG GTCCTTCAAC CTTGCAGCGA GGGGACAGTT TCCTAAAGCC AGAGAAGCCA 
TCCACCAGTC ACCAGCGGCC ACTGAGAAGC TTCCCCAAGA TTCCTTCCAA GTCTAAGTCA 
GTGGAGAGAC CAGATGAGGA ATTTCAGTTT CCTAAAGAGC TCATGGAAGA CTGGAGCACC 
ATGGAGGTGT GTGTGGACTG TAAAAAGTTT ATCTCGGAAA TTATCAGTTC TAGTCGGCGC 
AGTTTAACAC TCGCTAACAA GAGGGCTCGC TTAAAACGGA AGACGCAATC GTTCTACTTG 
TCCTCGACAG CAGCCTCGGA GTTTCGGCCC ACTGAGAGAA CAATCAATGA GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_031903690.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759550.1 Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa60054
Clone ID Related Accession (Same CDS sequence)	XM_031903690.1
Accession Version	XM_031903690.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein spire homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGT CTCAAGCGGC GCCTCGGAAA CGTAGTGAGG CTCAGGGCAG CGGGACTGGC 
GCGGCGGGAG ATGGGGAGTG TTTCTCGCTG GAGGAGATAT TGGATCTATA CAAGCAGCCG 
ATCAATGAGG AGCAAGCTTG GGCTGTGTGC TACCAGTGCT GCGGGGCCCT GCTGGAGGGG 
AGGGGGGGTG GCATTAAGGG AAGGAAGCTA ACTGGAGCCG CCGAGATTTG TATTCGTAAG 
GATGGAGCTG TCATCACCAG GCAGGAGTCG GCGGCAGCAG AGTCGATTGA TTGCAGGTTT 
ACAGAAACAG AGGTTATTGT GTCTCTTGGG ATCATCATTT ACAAAGCGTT GGATTATGGC 
TTGAAGGAGA ATGAGGAAAG AGAGCTTAGC CCTCCTTTGG AGCTACTGAT TGACAACATG 
ACTAACACAG ACATAGCTGG AAATAATGAT GAAGGCTATG ATGCACTGGA TGAAGATGAG 
ACCGACAGTG AAAGAGAATC AGCAGGATTC CGACACTCTT ACAGAAATGT AATGAAGCTT 
TGTACTACTC ATTTGCCAAC CCCATCAGAA GCACCCAATC ACTACCAAGC AGTATGCCGG 
GCCTTGTATG CAGAGACTAT GGAGTTACAC ATGTTCCTGA CCAAAATTAA AAGTGCAAAA 
GAGAGTTTAA AGAAGATTCA AGAAATGGAA AGAGATACAA CAGACAGAGC TTGCTCTGAT 
TTAGATGAGC TGAAAAACGC AGACTGGGCT CGGTTTTGGG TGCAGGTCAT GCGGGATCTA 
AGGAACGGTG TCAAGCTAAA GAAAGTGCAA GAGCGGCAGT ACGACCTGTT ACCAATAGAG 
TTTGAGCTGA CACCATATGA GATGCTTATG GATGATATTC GATCTAAACG ATACACCTTA 
AGGAAAGTCA TGGTTCAGGG TGACATCCCA CCCCGATTAA AGAAAAGTGC TCATGAAATA 
ATCCTGGATT TTATACGATC TAGACCTCCA CTTAATCCTG TAGCTGCTCG AAAGCTGAAA 
CCCACCCCAC CCCGTCCACG AAGCCTCCAT GAACAGATCC TGGCAGAGAT CAAGTCAGAG 
CGAAAGCTGC GTCCTGTTTC CCCTGATGAG ATTAGGCGCA GCAGATTAGT AACGCGGCCT 
CTTAGCATGT CTCACAGTTT TGACTTGTCA GATGTATCTA CTCCTGAAGC AACAAGAAAA 
GTGTCTGACA AAACAGTTTT AAATGGTGGG GTGACGCCTA AAGCCAAGGT CAATGGGCAC 
CCGATGCAGG TGGCAGTACA GAGGAAGAAG CTCCTGAAGG CTCCAGCCTT GGCCGAGTTT 
GAGAGTTCAG AATCAGAGGA GGACACAGTG CACAAATCAA CGAGCAGCAG TAGTGTGTCT 
ACTTCCCAGC CAGATGATGT CTCCCCGGAA TCTGCATCCA GCAGGAAATC TCCCCCGAAA 
TTCCTACCCA TATCTTCTAC CCCTCAACCT GAGAGACGTC AAGCTCCTCA AAGACGCCAC 
TCTATTGAGA AGGAGACACC AACCAACGTA CGTCAGTTCA TTCCTCCATC ACGACAGAGC 
TCGAGATCCC TGGTCCCTAG GATAACCCGT TTTTTTCAAA GGATGCCATT TCGACCATTT 
TCCTCCATAC ACACAGCTTC CCTGCTGGGC TCTCATCTTT TTGAAGCAGC TGTATTTGGG 
GCAGCAGGGG CTGTGTTTTA TCTGTTTGGG AAAGCTTATT CCAGCAGGTG GAGACACCCC 
CAGGAGGAGT TCTGCTACCC GGTGGAATGC CTGGCCCTCA CAGTGGAGGA GGTCATGCAC 
ATACGGCAGG TGCTGGTTAA AGCTGAGTTG GAGAAGTTCC AGCAGTATAA AGATATCTAT 
AATGCCCTGA AGAAAGGAAA GCTTTGTTTG TGCTGTCGAA CAAAAAGGTT TTCATTATTT 
ACATGGTCAT ACACTTGCCA GTTTTGCAAA AGACCAGTGT GCTCACAATG TTGCAAAAAG 
ATGAGATTAC CTTCTAAGCC ATATTCAACA CTTCCCATCT TCTCCCTGGG TCCTTCAACC 
TTGCAGCGAG GGGACAGTTT CCTAAAGCCA GAGAAGCCAT CCACCAGTCA CCAGCGGCCA 
CTGAGAAGCT TCCCCAAGAT TCCTTCCAAG TCTAAGTCAG TGGAGAGACC AGATGAGGAA 
TTTCAGTTTC CTAAAGAGCT CATGGAAGAC TGGAGCACCA TGGAGGTGTG TGTGGACTGT 
AAAAAGTTTA TCTCGGAAAT TATCAGTTCT AGTCGGCGCA GTTTAACACT CGCTAACAAG 
AGGGCTCGCT TAAAACGGAA GACGCAATCG TTCTACTTGT CCTCGACAGC AGCCTCGGAG 
TTTCGGCCCA CTGAGAGAAC AATCAATGAG ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759550.1
CDS	93..2468
Translation	MAESQAAPRKRSEAQGSGTGAAGDGECFSLEEILDLYKQPINEEQAWAVCYQCCGALLEGRGGGIKGRKLTGAAEICIRKDGAVITRQESAAAESIDCRFTETEVIVSLGIIIYKALDYGLKENEERELSPPLELLIDNMTNTDIAGNNDEGYDALDEDETDSERESAGFRHSYRNVMKLCTTHLPTPSEAPNHYQAVCRALYAETMELHMFLTKIKSAKESLKKIQEMERDTTDRACSDLDELKNADWARFWVQVMRDLRNGVKLKKVQERQYDLLPIEFELTPYEMLMDDIRSKRYTLRKVMVQGDIPPRLKKSAHEIILDFIRSRPPLNPVAARKLKPTPPRPRSLHEQILAEIKSERKLRPVSPDEIRRSRLVTRPLSMSHSFDLSDVSTPEATRKVSDKTVLNGGVTPKAKVNGHPMQVAVQRKKLLKAPALAEFESSESEEDTVHKSTSSSSVSTSQPDDVSPESASSRKSPPKFLPISSTPQPERRQAPQRRHSIEKETPTNVRQFIPPSRQSSRSLVPRITRFFQRMPFRPFSSIHTASLLGSHLFEAAVFGAAGAVFYLFGKAYSSRWRHPQEEFCYPVECLALTVEEVMHIRQVLVKAELEKFQQYKDIYNALKKGKLCLCCRTKRFSLFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSTLQRGDSFLKPEKPSTSHQRPLRSFPKIPSKSKSVERPDEEFQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLTLANKRARLKRKTQSFYLSSTAASEFRPTERTINEI

Target ORF information:

RefSeq Version	XM_031903690.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903690.1

ORF Insert Sequence:

ATGGCCGAGT CTCAAGCGGC GCCTCGGAAA CGTAGTGAGG CTCAGGGCAG CGGGACTGGC 
GCGGCGGGAG ATGGGGAGTG TTTCTCGCTG GAGGAGATAT TGGATCTATA CAAGCAGCCG 
ATCAATGAGG AGCAAGCTTG GGCTGTGTGC TACCAGTGCT GCGGGGCCCT GCTGGAGGGG 
AGGGGGGGTG GCATTAAGGG AAGGAAGCTA ACTGGAGCCG CCGAGATTTG TATTCGTAAG 
GATGGAGCTG TCATCACCAG GCAGGAGTCG GCGGCAGCAG AGTCGATTGA TTGCAGGTTT 
ACAGAAACAG AGGTTATTGT GTCTCTTGGG ATCATCATTT ACAAAGCGTT GGATTATGGC 
TTGAAGGAGA ATGAGGAAAG AGAGCTTAGC CCTCCTTTGG AGCTACTGAT TGACAACATG 
ACTAACACAG ACATAGCTGG AAATAATGAT GAAGGCTATG ATGCACTGGA TGAAGATGAG 
ACCGACAGTG AAAGAGAATC AGCAGGATTC CGACACTCTT ACAGAAATGT AATGAAGCTT 
TGTACTACTC ATTTGCCAAC CCCATCAGAA GCACCCAATC ACTACCAAGC AGTATGCCGG 
GCCTTGTATG CAGAGACTAT GGAGTTACAC ATGTTCCTGA CCAAAATTAA AAGTGCAAAA 
GAGAGTTTAA AGAAGATTCA AGAAATGGAA AGAGATACAA CAGACAGAGC TTGCTCTGAT 
TTAGATGAGC TGAAAAACGC AGACTGGGCT CGGTTTTGGG TGCAGGTCAT GCGGGATCTA 
AGGAACGGTG TCAAGCTAAA GAAAGTGCAA GAGCGGCAGT ACGACCTGTT ACCAATAGAG 
TTTGAGCTGA CACCATATGA GATGCTTATG GATGATATTC GATCTAAACG ATACACCTTA 
AGGAAAGTCA TGGTTCAGGG TGACATCCCA CCCCGATTAA AGAAAAGTGC TCATGAAATA 
ATCCTGGATT TTATACGATC TAGACCTCCA CTTAATCCTG TAGCTGCTCG AAAGCTGAAA 
CCCACCCCAC CCCGTCCACG AAGCCTCCAT GAACAGATCC TGGCAGAGAT CAAGTCAGAG 
CGAAAGCTGC GTCCTGTTTC CCCTGATGAG ATTAGGCGCA GCAGATTAGT AACGCGGCCT 
CTTAGCATGT CTCACAGTTT TGACTTGTCA GATGTATCTA CTCCTGAAGC AACAAGAAAA 
GTGTCTGACA AAACAGTTTT AAATGGTGGG GTGACGCCTA AAGCCAAGGT CAATGGGCAC 
CCGATGCAGG TGGCAGTACA GAGGAAGAAG CTCCTGAAGG CTCCAGCCTT GGCCGAGTTT 
GAGAGTTCAG AATCAGAGGA GGACACAGTG CACAAATCAA CGAGCAGCAG TAGTGTGTCT 
ACTTCCCAGC CAGATGATGT CTCCCCGGAA TCTGCATCCA GCAGGAAATC TCCCCCGAAA 
TTCCTACCCA TATCTTCTAC CCCTCAACCT GAGAGACGTC AAGCTCCTCA AAGACGCCAC 
TCTATTGAGA AGGAGACACC AACCAACGTA CGTCAGTTCA TTCCTCCATC ACGACAGAGC 
TCGAGATCCC TGGTCCCTAG GATAACCCGT TTTTTTCAAA GGATGCCATT TCGACCATTT 
TCCTCCATAC ACACAGCTTC CCTGCTGGGC TCTCATCTTT TTGAAGCAGC TGTATTTGGG 
GCAGCAGGGG CTGTGTTTTA TCTGTTTGGG AAAGCTTATT CCAGCAGGTG GAGACACCCC 
CAGGAGGAGT TCTGCTACCC GGTGGAATGC CTGGCCCTCA CAGTGGAGGA GGTCATGCAC 
ATACGGCAGG TGCTGGTTAA AGCTGAGTTG GAGAAGTTCC AGCAGTATAA AGATATCTAT 
AATGCCCTGA AGAAAGGAAA GCTTTGTTTG TGCTGTCGAA CAAAAAGGTT TTCATTATTT 
ACATGGTCAT ACACTTGCCA GTTTTGCAAA AGACCAGTGT GCTCACAATG TTGCAAAAAG 
ATGAGATTAC CTTCTAAGCC ATATTCAACA CTTCCCATCT TCTCCCTGGG TCCTTCAACC 
TTGCAGCGAG GGGACAGTTT CCTAAAGCCA GAGAAGCCAT CCACCAGTCA CCAGCGGCCA 
CTGAGAAGCT TCCCCAAGAT TCCTTCCAAG TCTAAGTCAG TGGAGAGACC AGATGAGGAA 
TTTCAGTTTC CTAAAGAGCT CATGGAAGAC TGGAGCACCA TGGAGGTGTG TGTGGACTGT 
AAAAAGTTTA TCTCGGAAAT TATCAGTTCT AGTCGGCGCA GTTTAACACT CGCTAACAAG 
AGGGCTCGCT TAAAACGGAA GACGCAATCG TTCTACTTGT CCTCGACAGC AGCCTCGGAG 
TTTCGGCCCA CTGAGAGAAC AATCAATGAG ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_031903691.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759551.1 Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa60055
Clone ID Related Accession (Same CDS sequence)	XM_031903691.1
Accession Version	XM_031903691.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein spire homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGT CTCAAGCGGC GCCTCGGAAA CGTAGTGAGG CTCAGGGCAG CGGGACTGGC 
GCGGCGGGAG ATGGGGAGTG TTTCTCGCTG GAGGAGATAT TGGATCTATA CAAGCAGCCG 
ATCAATGAGG AGCAAGCTTG GGCTGTGTGC TACCAGTGCT GCGGGGCCCT GCTGGAGGGG 
AGGGGGGGTG GCATTAAGGG AAGGAAGCTA ACTGGAGCCG CCGAGATTTG TATTCGTAAG 
GATGGAGCTG TCATCACCAG GCAGGAGTCG GCGGCAGCAG AGTCGATTGA TTGCAGGTTT 
ACAGAAACAG AGGTTATTGT GTCTCTTGGG ATCATCATTT ACAAAGCGTT GGATTATGGC 
TTGAAGGAGA ATGAGGAAAG AGAGCTTAGC CCTCCTTTGG AGCTACTGAT TGACAACATG 
ACTAACACAG ACATAGCTGG AAATAATGAT GAAGGCTATG ATGCACTGGA TGAAGATGAG 
ACCGACAGTG AAAGAGAATC AGCAGGATTC CGACACTCTT ACAGAAATGT AATGAAGCTT 
TGTACTACTC ATTTGCCAAC CCCATCAGAA GCACCCAATC ACTACCAAGC AGTATGCCGG 
GCCTTGTATG CAGAGACTAT GGAGTTACAC ATGTTCCTGA CCAAAATTAA AAGTGCAAAA 
GAGAGTTTAA AGAAGATTCA AGAAATGGAA AGAGATACAA CAGACAGAGC TTGCTCTGAT 
TTAGATGAGC TGAAAAACGC AGACTGGGCT CGGTTTTGGG TGCAGGTCAT GCGGGATCTA 
AGGAACGGTG TCAAGCTAAA GAAAGTGCAA GAGCGGCAGT ACGACCTGTT ACCAATAGAG 
TTTGAGCTGA CACCATATGA GATGCTTATG GATGATATTC GATCTAAACG ATACACCTTA 
AGGAAAGTCA TGGTTCAGGG TGACATCCCA CCCCGATTAA AGAAAAGTGC TCATGAAATA 
ATCCTGGATT TTATACGATC TAGACCTCCA CTTAATCCTG TAGCTGCTCG AAAGCTGAAA 
CCCACCCCAC CCCGTCCACG AAGCCTCCAT GAACAGATCC TGGCAGAGAT CAAGTCAGAG 
CGAAAGCTGC GTCCTGTTTC CCCTGATGAG ATTAGGCGCA GCAGATTAGA TGTATCTACT 
CCTGAAGCAA CAAGAAAAGT GTCTGACAAA ACAGTTTTAA ATGGTGGGGT GACGCCTAAA 
GCCAAGGTCA ATGGGCACCC GATGCAGGTG GCAGTACAGA GGAAGAAGCT CCTGAAGGCT 
CCAGCCTTGG CCGAGTTTGA GAGTTCAGAA TCAGAGGAGG ACACAGTGCA CAAATCAACG 
AGCAGCAGTA GTGTGTCTAC TTCCCAGCCA GATGATGTCT CCCCGGAATC TGCATCCAGC 
AGGAAATCTC CCCCGAAATT CCTACCCATA TCTTCTACCC CTCAACCTGA GAGACGTCAA 
GCTCCTCAAA GACGCCACTC TATTGAGAAG GAGACACCAA CCAACGTACG TCAGTTCATT 
CCTCCATCAC GACAGAGCTC GAGATCCCTG GTCCCTAGGA TAACCCGTTT TTTTCAAAGG 
ATGCCATTTC GACCATTTTC CTCCATACAC ACAGCTTCCC TGCTGGGCTC TCATCTTTTT 
GAAGCAGCTG TATTTGGGGC AGCAGGGGCT GTGTTTTATC TGTTTGGGAA AGCTTATTCC 
AGCAGGTGGA GACACCCCCA GGAGGAGTTC TGCTACCCGG TGGAATGCCT GGCCCTCACA 
GTGGAGGAGG TCATGCACAT ACGGCAGGTG CTGGTTAAAG CTGAGTTGGA GAAGTTCCAG 
CAGTATAAAG ATATCTATAA TGCCCTGAAG AAAGGAAAGC TTTGTTTGTG CTGTCGAACA 
AAAAGGTTTT CATTATTTAC ATGGTCATAC ACTTGCCAGT TTTGCAAAAG ACCAGTGTGC 
TCACAATGTT GCAAAAAGAT GAGATTACCT TCTAAGCCAT ATTCAACACT TCCCATCTTC 
TCCCTGGGTC CTTCAACCTT GCAGCGAGGG GACAGTTTCC TAAAGCCAGA GAAGCCATCC 
ACCAGTCACC AGCGGCCACT GAGAAGCTTC CCCAAGATTC CTTCCAAGTC TAAGTCAGTG 
GAGAGACCAG ATGAGGAATT TCAGTTTCCT AAAGAGCTCA TGGAAGACTG GAGCACCATG 
GAGGTGTGTG TGGACTGTAA AAAGTTTATC TCGGAAATTA TCAGTTCTAG TCGGCGCAGT 
TTAACACTCG CTAACAAGAG GGCTCGCTTA AAACGGAAGA CGCAATCGTT CTACTTGTCC 
TCGACAGCAG CCTCGGAGTT TCGGCCCACT GAGAGAACAA TCAATGAGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759551.1
CDS	93..2426
Translation	MAESQAAPRKRSEAQGSGTGAAGDGECFSLEEILDLYKQPINEEQAWAVCYQCCGALLEGRGGGIKGRKLTGAAEICIRKDGAVITRQESAAAESIDCRFTETEVIVSLGIIIYKALDYGLKENEERELSPPLELLIDNMTNTDIAGNNDEGYDALDEDETDSERESAGFRHSYRNVMKLCTTHLPTPSEAPNHYQAVCRALYAETMELHMFLTKIKSAKESLKKIQEMERDTTDRACSDLDELKNADWARFWVQVMRDLRNGVKLKKVQERQYDLLPIEFELTPYEMLMDDIRSKRYTLRKVMVQGDIPPRLKKSAHEIILDFIRSRPPLNPVAARKLKPTPPRPRSLHEQILAEIKSERKLRPVSPDEIRRSRLDVSTPEATRKVSDKTVLNGGVTPKAKVNGHPMQVAVQRKKLLKAPALAEFESSESEEDTVHKSTSSSSVSTSQPDDVSPESASSRKSPPKFLPISSTPQPERRQAPQRRHSIEKETPTNVRQFIPPSRQSSRSLVPRITRFFQRMPFRPFSSIHTASLLGSHLFEAAVFGAAGAVFYLFGKAYSSRWRHPQEEFCYPVECLALTVEEVMHIRQVLVKAELEKFQQYKDIYNALKKGKLCLCCRTKRFSLFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSTLQRGDSFLKPEKPSTSHQRPLRSFPKIPSKSKSVERPDEEFQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLTLANKRARLKRKTQSFYLSSTAASEFRPTERTINEI

Target ORF information:

RefSeq Version	XM_031903691.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903691.1

ORF Insert Sequence:

ATGGCCGAGT CTCAAGCGGC GCCTCGGAAA CGTAGTGAGG CTCAGGGCAG CGGGACTGGC 
GCGGCGGGAG ATGGGGAGTG TTTCTCGCTG GAGGAGATAT TGGATCTATA CAAGCAGCCG 
ATCAATGAGG AGCAAGCTTG GGCTGTGTGC TACCAGTGCT GCGGGGCCCT GCTGGAGGGG 
AGGGGGGGTG GCATTAAGGG AAGGAAGCTA ACTGGAGCCG CCGAGATTTG TATTCGTAAG 
GATGGAGCTG TCATCACCAG GCAGGAGTCG GCGGCAGCAG AGTCGATTGA TTGCAGGTTT 
ACAGAAACAG AGGTTATTGT GTCTCTTGGG ATCATCATTT ACAAAGCGTT GGATTATGGC 
TTGAAGGAGA ATGAGGAAAG AGAGCTTAGC CCTCCTTTGG AGCTACTGAT TGACAACATG 
ACTAACACAG ACATAGCTGG AAATAATGAT GAAGGCTATG ATGCACTGGA TGAAGATGAG 
ACCGACAGTG AAAGAGAATC AGCAGGATTC CGACACTCTT ACAGAAATGT AATGAAGCTT 
TGTACTACTC ATTTGCCAAC CCCATCAGAA GCACCCAATC ACTACCAAGC AGTATGCCGG 
GCCTTGTATG CAGAGACTAT GGAGTTACAC ATGTTCCTGA CCAAAATTAA AAGTGCAAAA 
GAGAGTTTAA AGAAGATTCA AGAAATGGAA AGAGATACAA CAGACAGAGC TTGCTCTGAT 
TTAGATGAGC TGAAAAACGC AGACTGGGCT CGGTTTTGGG TGCAGGTCAT GCGGGATCTA 
AGGAACGGTG TCAAGCTAAA GAAAGTGCAA GAGCGGCAGT ACGACCTGTT ACCAATAGAG 
TTTGAGCTGA CACCATATGA GATGCTTATG GATGATATTC GATCTAAACG ATACACCTTA 
AGGAAAGTCA TGGTTCAGGG TGACATCCCA CCCCGATTAA AGAAAAGTGC TCATGAAATA 
ATCCTGGATT TTATACGATC TAGACCTCCA CTTAATCCTG TAGCTGCTCG AAAGCTGAAA 
CCCACCCCAC CCCGTCCACG AAGCCTCCAT GAACAGATCC TGGCAGAGAT CAAGTCAGAG 
CGAAAGCTGC GTCCTGTTTC CCCTGATGAG ATTAGGCGCA GCAGATTAGA TGTATCTACT 
CCTGAAGCAA CAAGAAAAGT GTCTGACAAA ACAGTTTTAA ATGGTGGGGT GACGCCTAAA 
GCCAAGGTCA ATGGGCACCC GATGCAGGTG GCAGTACAGA GGAAGAAGCT CCTGAAGGCT 
CCAGCCTTGG CCGAGTTTGA GAGTTCAGAA TCAGAGGAGG ACACAGTGCA CAAATCAACG 
AGCAGCAGTA GTGTGTCTAC TTCCCAGCCA GATGATGTCT CCCCGGAATC TGCATCCAGC 
AGGAAATCTC CCCCGAAATT CCTACCCATA TCTTCTACCC CTCAACCTGA GAGACGTCAA 
GCTCCTCAAA GACGCCACTC TATTGAGAAG GAGACACCAA CCAACGTACG TCAGTTCATT 
CCTCCATCAC GACAGAGCTC GAGATCCCTG GTCCCTAGGA TAACCCGTTT TTTTCAAAGG 
ATGCCATTTC GACCATTTTC CTCCATACAC ACAGCTTCCC TGCTGGGCTC TCATCTTTTT 
GAAGCAGCTG TATTTGGGGC AGCAGGGGCT GTGTTTTATC TGTTTGGGAA AGCTTATTCC 
AGCAGGTGGA GACACCCCCA GGAGGAGTTC TGCTACCCGG TGGAATGCCT GGCCCTCACA 
GTGGAGGAGG TCATGCACAT ACGGCAGGTG CTGGTTAAAG CTGAGTTGGA GAAGTTCCAG 
CAGTATAAAG ATATCTATAA TGCCCTGAAG AAAGGAAAGC TTTGTTTGTG CTGTCGAACA 
AAAAGGTTTT CATTATTTAC ATGGTCATAC ACTTGCCAGT TTTGCAAAAG ACCAGTGTGC 
TCACAATGTT GCAAAAAGAT GAGATTACCT TCTAAGCCAT ATTCAACACT TCCCATCTTC 
TCCCTGGGTC CTTCAACCTT GCAGCGAGGG GACAGTTTCC TAAAGCCAGA GAAGCCATCC 
ACCAGTCACC AGCGGCCACT GAGAAGCTTC CCCAAGATTC CTTCCAAGTC TAAGTCAGTG 
GAGAGACCAG ATGAGGAATT TCAGTTTCCT AAAGAGCTCA TGGAAGACTG GAGCACCATG 
GAGGTGTGTG TGGACTGTAA AAAGTTTATC TCGGAAATTA TCAGTTCTAG TCGGCGCAGT 
TTAACACTCG CTAACAAGAG GGCTCGCTTA AAACGGAAGA CGCAATCGTT CTACTTGTCC 
TCGACAGCAG CCTCGGAGTT TCGGCCCACT GAGAGAACAA TCAATGAGAT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_031903692.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759552.1 Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa60056
Clone ID Related Accession (Same CDS sequence)	XM_031903692.1
Accession Version	XM_031903692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein spire homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGT CTCAAGCGGC GCCTCGGAAA CGTAGTGAGG CTCAGGGCAG CGGGACTGGC 
GCGGCGGGAG ATGGGGAGTG TTTCTCGCTG GAGGAGATAT TGGATCTATA CAAGCAGCCG 
ATCAATGAGG AGCAAGCTTG GGCTGTGTGC TACCAGTGCT GCGGGGCCCT GCTGGAGGGG 
AGGGGGGGTG GCATTAAGGG AAGGAAGCTA ACTGGAGCCG CCGAGATTTG TATTCGTAAG 
GATGGAGCTG TCATCACCAG GCAGGAGTCG GCGGCAGCAG AGTCGATTGA TTGCAGGTTT 
ACAGAAACAG AGGTTATTGT GTCTCTTGGG ATCATCATTT ACAAAGCGTT GGATTATGGC 
TTGAAGGAGA ATGAGGAAAG AGAGCTTAGC CCTCCTTTGG AGCTACTGAT TGACAACATG 
ACTAACACAG ACATAGCTGG AAATAATGAT GAAGGCTATG ATGCACTGGA TGAAGATGAG 
ACCGACAGTG AAAGAGAATC AGCAGGATTC CGACACTCTT ACAGAAATGT AATGAAGCTT 
TGTACTACTC ATTTGCCAAC CCCATCAGAA GCACCCAATC ACTACCAAGC AGTATGCCGG 
GCCTTGTATG CAGAGACTAT GGAGTTACAC ATGTTCCTGA CCAAAATTAA AAGTGCAAAA 
GAGAGTTTAA AGAAGATTCA AGAAATGGAA AGAGATACAA CAGACAGAGC TTGCTCTGAT 
TTAGATGAGC TGAAAAACGC AGACTGGGCT CGGTTTTGGG TGCAGGTCAT GCGGGATCTA 
AGGAACGGTG TCAAGCTAAA GAAAGTGCAA GAGCGGCAGT ACGACCTGTT ACCAATAGAG 
TTTGAGCTGA CACCATATGA GATGCTTATG GATGATATTC GATCTAAACG ATACACCTTA 
AGGAAAGTCA TGGTTCAGGG TGACATCCCA CCCCGATTAA AGAAAAGTGC TCATGAAATA 
ATCCTGGATT TTATACGATC TAGACCTCCA CTTAATCCTG TAGCTGCTCG AAAGCTGAAA 
CCCACCCCAC CCCGTCCACG AAGCCTCCAT GAACAGATCC TGGCAGAGAT CAAGTCAGAG 
CGAAAGCTGC GTCCTGTTTC CCCTGATGAG ATTAGGCGCA GCAGATTAGT AACGCGGCCT 
CTTAGCATGT CTCACAGTTT TGACTTGTCA GATGTATCTA CTCCTGAAGC AACAAGAAAA 
GTGTCTGACA AAACAGTTTT AAATGGTGGG GTGACGCCTA AAGCCAAGGT CAATGGGCAC 
CCGATGCAGG TGGCAGTACA GAGGAAGAAG CTCCTGAAGG CTCCAGCCTT GGCCGAGTTT 
GAGAGTTCAG AATCAGAGGA GGACACAGTG CACAAATCAA CGAGCAGCAG TAGTGTGTCT 
ACTTCCCAGC CAGATGATGT CTCCCCGGAA TCTGCATCCA GCAGGAAATC TCCCCCGAAA 
TTCCTACCCA TATCTTCTAC CCCTCAACCT GAGAGACGTC AAGCTCCTCA AAGACGCCAC 
TCTATTGAGA AGGAGACACC AACCAACGTA CGTCAGTTCA TTCCTCCATC ACGACAGAGC 
TCGAGATCCC TGGGTCTCCA GGTCACAGAA TTACCCAGTG GGGAGGAGTT CTGCTACCCG 
GTGGAATGCC TGGCCCTCAC AGTGGAGGAG GTCATGCACA TACGGCAGGT GCTGGTTAAA 
GCTGAGTTGG AGAAGTTCCA GCAGTATAAA GATATCTATA ATGCCCTGAA GAAAGGAAAG 
CTTTGTTTGT GCTGTCGAAC AAAAAGGTTT TCATTATTTA CATGGTCATA CACTTGCCAG 
TTTTGCAAAA GACCAGTGTG CTCACAATGT TGCAAAAAGA TGAGATTACC TTCTAAGCCA 
TATTCAACAC TTCCCATCTT CTCCCTGGGT CCTTCAACCT TGCAGCGAGG GGACAGTTTC 
CTAAAGCCAG AGAAGCCATC CACCAGTCAC CAGCGGCCAC TGAGAAGCTT CCCCAAGATT 
CCTTCCAAGT CTAAGTCAGT GGAGAGACCA GATGAGGAAT TTCAGTTTCC TAAAGAGCTC 
ATGGAAGACT GGAGCACCAT GGAGGTGTGT GTGGACTGTA AAAAGTTTAT CTCGGAAATT 
ATCAGTTCTA GTCGGCGCAG TTTAACACTC GCTAACAAGA GGGCTCGCTT AAAACGGAAG 
ACGCAATCGT TCTACTTGTC CTCGACAGCA GCCTCGGAGT TTCGGCCCAC TGAGAGAACA 
ATCAATGAGA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759552.1
CDS	93..2327
Translation	MAESQAAPRKRSEAQGSGTGAAGDGECFSLEEILDLYKQPINEEQAWAVCYQCCGALLEGRGGGIKGRKLTGAAEICIRKDGAVITRQESAAAESIDCRFTETEVIVSLGIIIYKALDYGLKENEERELSPPLELLIDNMTNTDIAGNNDEGYDALDEDETDSERESAGFRHSYRNVMKLCTTHLPTPSEAPNHYQAVCRALYAETMELHMFLTKIKSAKESLKKIQEMERDTTDRACSDLDELKNADWARFWVQVMRDLRNGVKLKKVQERQYDLLPIEFELTPYEMLMDDIRSKRYTLRKVMVQGDIPPRLKKSAHEIILDFIRSRPPLNPVAARKLKPTPPRPRSLHEQILAEIKSERKLRPVSPDEIRRSRLVTRPLSMSHSFDLSDVSTPEATRKVSDKTVLNGGVTPKAKVNGHPMQVAVQRKKLLKAPALAEFESSESEEDTVHKSTSSSSVSTSQPDDVSPESASSRKSPPKFLPISSTPQPERRQAPQRRHSIEKETPTNVRQFIPPSRQSSRSLGLQVTELPSGEEFCYPVECLALTVEEVMHIRQVLVKAELEKFQQYKDIYNALKKGKLCLCCRTKRFSLFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSTLQRGDSFLKPEKPSTSHQRPLRSFPKIPSKSKSVERPDEEFQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLTLANKRARLKRKTQSFYLSSTAASEFRPTERTINEI

Target ORF information:

RefSeq Version	XM_031903692.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903692.1

ORF Insert Sequence:

ATGGCCGAGT CTCAAGCGGC GCCTCGGAAA CGTAGTGAGG CTCAGGGCAG CGGGACTGGC 
GCGGCGGGAG ATGGGGAGTG TTTCTCGCTG GAGGAGATAT TGGATCTATA CAAGCAGCCG 
ATCAATGAGG AGCAAGCTTG GGCTGTGTGC TACCAGTGCT GCGGGGCCCT GCTGGAGGGG 
AGGGGGGGTG GCATTAAGGG AAGGAAGCTA ACTGGAGCCG CCGAGATTTG TATTCGTAAG 
GATGGAGCTG TCATCACCAG GCAGGAGTCG GCGGCAGCAG AGTCGATTGA TTGCAGGTTT 
ACAGAAACAG AGGTTATTGT GTCTCTTGGG ATCATCATTT ACAAAGCGTT GGATTATGGC 
TTGAAGGAGA ATGAGGAAAG AGAGCTTAGC CCTCCTTTGG AGCTACTGAT TGACAACATG 
ACTAACACAG ACATAGCTGG AAATAATGAT GAAGGCTATG ATGCACTGGA TGAAGATGAG 
ACCGACAGTG AAAGAGAATC AGCAGGATTC CGACACTCTT ACAGAAATGT AATGAAGCTT 
TGTACTACTC ATTTGCCAAC CCCATCAGAA GCACCCAATC ACTACCAAGC AGTATGCCGG 
GCCTTGTATG CAGAGACTAT GGAGTTACAC ATGTTCCTGA CCAAAATTAA AAGTGCAAAA 
GAGAGTTTAA AGAAGATTCA AGAAATGGAA AGAGATACAA CAGACAGAGC TTGCTCTGAT 
TTAGATGAGC TGAAAAACGC AGACTGGGCT CGGTTTTGGG TGCAGGTCAT GCGGGATCTA 
AGGAACGGTG TCAAGCTAAA GAAAGTGCAA GAGCGGCAGT ACGACCTGTT ACCAATAGAG 
TTTGAGCTGA CACCATATGA GATGCTTATG GATGATATTC GATCTAAACG ATACACCTTA 
AGGAAAGTCA TGGTTCAGGG TGACATCCCA CCCCGATTAA AGAAAAGTGC TCATGAAATA 
ATCCTGGATT TTATACGATC TAGACCTCCA CTTAATCCTG TAGCTGCTCG AAAGCTGAAA 
CCCACCCCAC CCCGTCCACG AAGCCTCCAT GAACAGATCC TGGCAGAGAT CAAGTCAGAG 
CGAAAGCTGC GTCCTGTTTC CCCTGATGAG ATTAGGCGCA GCAGATTAGT AACGCGGCCT 
CTTAGCATGT CTCACAGTTT TGACTTGTCA GATGTATCTA CTCCTGAAGC AACAAGAAAA 
GTGTCTGACA AAACAGTTTT AAATGGTGGG GTGACGCCTA AAGCCAAGGT CAATGGGCAC 
CCGATGCAGG TGGCAGTACA GAGGAAGAAG CTCCTGAAGG CTCCAGCCTT GGCCGAGTTT 
GAGAGTTCAG AATCAGAGGA GGACACAGTG CACAAATCAA CGAGCAGCAG TAGTGTGTCT 
ACTTCCCAGC CAGATGATGT CTCCCCGGAA TCTGCATCCA GCAGGAAATC TCCCCCGAAA 
TTCCTACCCA TATCTTCTAC CCCTCAACCT GAGAGACGTC AAGCTCCTCA AAGACGCCAC 
TCTATTGAGA AGGAGACACC AACCAACGTA CGTCAGTTCA TTCCTCCATC ACGACAGAGC 
TCGAGATCCC TGGGTCTCCA GGTCACAGAA TTACCCAGTG GGGAGGAGTT CTGCTACCCG 
GTGGAATGCC TGGCCCTCAC AGTGGAGGAG GTCATGCACA TACGGCAGGT GCTGGTTAAA 
GCTGAGTTGG AGAAGTTCCA GCAGTATAAA GATATCTATA ATGCCCTGAA GAAAGGAAAG 
CTTTGTTTGT GCTGTCGAAC AAAAAGGTTT TCATTATTTA CATGGTCATA CACTTGCCAG 
TTTTGCAAAA GACCAGTGTG CTCACAATGT TGCAAAAAGA TGAGATTACC TTCTAAGCCA 
TATTCAACAC TTCCCATCTT CTCCCTGGGT CCTTCAACCT TGCAGCGAGG GGACAGTTTC 
CTAAAGCCAG AGAAGCCATC CACCAGTCAC CAGCGGCCAC TGAGAAGCTT CCCCAAGATT 
CCTTCCAAGT CTAAGTCAGT GGAGAGACCA GATGAGGAAT TTCAGTTTCC TAAAGAGCTC 
ATGGAAGACT GGAGCACCAT GGAGGTGTGT GTGGACTGTA AAAAGTTTAT CTCGGAAATT 
ATCAGTTCTA GTCGGCGCAG TTTAACACTC GCTAACAAGA GGGCTCGCTT AAAACGGAAG 
ACGCAATCGT TCTACTTGTC CTCGACAGCA GCCTCGGAGT TTCGGCCCAC TGAGAGAACA 
ATCAATGAGA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_031903693.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759553.1 Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa60057
Clone ID Related Accession (Same CDS sequence)	XM_031903693.1
Accession Version	XM_031903693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein spire homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGT CTCAAGCGGC GCCTCGGAAA CGTAGTGAGG CTCAGGGCAG CGGGACTGGC 
GCGGCGGGAG ATGGGGAGTG TTTCTCGCTG GAGGAGATAT TGGATCTATA CAAGCAGCCG 
ATCAATGAGG AGCAAGCTTG GGCTGTGTGC TACCAGTGCT GCGGGGCCCT GCTGGAGGGG 
AGGGGGGGTG GCATTAAGGG AAGGAAGCTA ACTGGAGCCG CCGAGATTTG TATTCGTAAG 
GATGGAGCTG TCATCACCAG GCAGGAGTCG GCGGCAGCAG AGTCGATTGA TTGCAGGTTT 
ACAGAAACAG AGGTTATTGT GTCTCTTGGG ATCATCATTT ACAAAGCGTT GGATTATGGC 
TTGAAGGAGA ATGAGGAAAG AGAGCTTAGC CCTCCTTTGG AGCTACTGAT TGACAACATG 
ACTAACACAG ACATAGCTGG AAATAATGAT GAAGGCTATG ATGCACTGGA TGAAGATGAG 
ACCGACAGTG AAAGAGAATC AGCAGGATTC CGACACTCTT ACAGAAATGT AATGAAGCTT 
TGTACTACTC ATTTGCCAAC CCCATCAGAA GCACCCAATC ACTACCAAGC AGTATGCCGG 
GCCTTGTATG CAGAGACTAT GGAGTTACAC ATGTTCCTGA CCAAAATTAA AAGTGCAAAA 
GAGAGTTTAA AGAAGATTCA AGAAATGGAA AGAGATACAA CAGACAGAGC TTGCTCTGAT 
TTAGATGAGC TGAAAAACGC AGACTGGGCT CGGTTTTGGG TGCAGGTCAT GCGGGATCTA 
AGGAACGGTG TCAAGCTAAA GAAAGTGCAA GAGCGGCAGT ACGACCTGTT ACCAATAGAG 
TTTGAGCTGA CACCATATGA GATGCTTATG GATGATATTC GATCTAAACG ATACACCTTA 
AGGAAAGTCA TGGTTCAGGG TGACATCCCA CCCCGATTAA AGAAAAGTGC TCATGAAATA 
ATCCTGGATT TTATACGATC TAGACCTCCA CTTAATCCTG TAGCTGCTCG AAAGCTGAAA 
CCCACCCCAC CCCGTCCACG AAGCCTCCAT GAACAGATCC TGGCAGAGAT CAAGTCAGAG 
CGAAAGCTGC GTCCTGTTTC CCCTGATGAG ATTAGGCGCA GCAGATTAGT AACGCGGCCT 
CTTAGCATGT CTCACAGTTT TGACTTGTCA GATGTATCTA CTCCTGAAGC AACAAGAAAA 
GTGTCTGACA AAACAGTTTT AAATGGTGGG GTGACGCCTA AAGCCAAGGT CAATGGGCAC 
CCGATGCAGG TGGCAGTACA GAGGAAGAAG CTCCTGAAGG CTCCAGCCTT GGCCGAGTTT 
GAGAGTTCAG AATCAGAGGA GGACACAGTG CACAAATCAA CGAGCAGCAG TAGTGTGTCT 
ACTTCCCAGC CAGATGATGT CTCCCCGGAA TCTGCATCCA GCAGGAAATC TCCCCCGAAA 
TTCCTACCCA TATCTTCTAC CCCTCAACCT GAGAGACGTC AAGCTCCTCA AAGACGCCAC 
TCTATTGAGA AGGAGACACC AACCAACGTA CGTCAGTTCA TTCCTCCATC ACGACAGAGC 
TCGAGATCCC TGGAGGAGTT CTGCTACCCG GTGGAATGCC TGGCCCTCAC AGTGGAGGAG 
GTCATGCACA TACGGCAGGT GCTGGTTAAA GCTGAGTTGG AGAAGTTCCA GCAGTATAAA 
GATATCTATA ATGCCCTGAA GAAAGGAAAG CTTTGTTTGT GCTGTCGAAC AAAAAGGTTT 
TCATTATTTA CATGGTCATA CACTTGCCAG TTTTGCAAAA GACCAGTGTG CTCACAATGT 
TGCAAAAAGA TGAGATTACC TTCTAAGCCA TATTCAACAC TTCCCATCTT CTCCCTGGGT 
CCTTCAACCT TGCAGCGAGG GGACAGTTTC CTAAAGCCAG AGAAGCCATC CACCAGTCAC 
CAGCGGCCAC TGAGAAGCTT CCCCAAGATT CCTTCCAAGT CTAAGTCAGT GGAGAGACCA 
GATGAGGAAT TTCAGTTTCC TAAAGAGCTC ATGGAAGACT GGAGCACCAT GGAGGTGTGT 
GTGGACTGTA AAAAGTTTAT CTCGGAAATT ATCAGTTCTA GTCGGCGCAG TTTAACACTC 
GCTAACAAGA GGGCTCGCTT AAAACGGAAG ACGCAATCGT TCTACTTGTC CTCGACAGCA 
GCCTCGGAGT TTCGGCCCAC TGAGAGAACA ATCAATGAGA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759553.1
CDS	93..2297
Translation	MAESQAAPRKRSEAQGSGTGAAGDGECFSLEEILDLYKQPINEEQAWAVCYQCCGALLEGRGGGIKGRKLTGAAEICIRKDGAVITRQESAAAESIDCRFTETEVIVSLGIIIYKALDYGLKENEERELSPPLELLIDNMTNTDIAGNNDEGYDALDEDETDSERESAGFRHSYRNVMKLCTTHLPTPSEAPNHYQAVCRALYAETMELHMFLTKIKSAKESLKKIQEMERDTTDRACSDLDELKNADWARFWVQVMRDLRNGVKLKKVQERQYDLLPIEFELTPYEMLMDDIRSKRYTLRKVMVQGDIPPRLKKSAHEIILDFIRSRPPLNPVAARKLKPTPPRPRSLHEQILAEIKSERKLRPVSPDEIRRSRLVTRPLSMSHSFDLSDVSTPEATRKVSDKTVLNGGVTPKAKVNGHPMQVAVQRKKLLKAPALAEFESSESEEDTVHKSTSSSSVSTSQPDDVSPESASSRKSPPKFLPISSTPQPERRQAPQRRHSIEKETPTNVRQFIPPSRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKFQQYKDIYNALKKGKLCLCCRTKRFSLFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSTLQRGDSFLKPEKPSTSHQRPLRSFPKIPSKSKSVERPDEEFQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLTLANKRARLKRKTQSFYLSSTAASEFRPTERTINEI

Target ORF information:

RefSeq Version	XM_031903693.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903693.1

ORF Insert Sequence:

ATGGCCGAGT CTCAAGCGGC GCCTCGGAAA CGTAGTGAGG CTCAGGGCAG CGGGACTGGC 
GCGGCGGGAG ATGGGGAGTG TTTCTCGCTG GAGGAGATAT TGGATCTATA CAAGCAGCCG 
ATCAATGAGG AGCAAGCTTG GGCTGTGTGC TACCAGTGCT GCGGGGCCCT GCTGGAGGGG 
AGGGGGGGTG GCATTAAGGG AAGGAAGCTA ACTGGAGCCG CCGAGATTTG TATTCGTAAG 
GATGGAGCTG TCATCACCAG GCAGGAGTCG GCGGCAGCAG AGTCGATTGA TTGCAGGTTT 
ACAGAAACAG AGGTTATTGT GTCTCTTGGG ATCATCATTT ACAAAGCGTT GGATTATGGC 
TTGAAGGAGA ATGAGGAAAG AGAGCTTAGC CCTCCTTTGG AGCTACTGAT TGACAACATG 
ACTAACACAG ACATAGCTGG AAATAATGAT GAAGGCTATG ATGCACTGGA TGAAGATGAG 
ACCGACAGTG AAAGAGAATC AGCAGGATTC CGACACTCTT ACAGAAATGT AATGAAGCTT 
TGTACTACTC ATTTGCCAAC CCCATCAGAA GCACCCAATC ACTACCAAGC AGTATGCCGG 
GCCTTGTATG CAGAGACTAT GGAGTTACAC ATGTTCCTGA CCAAAATTAA AAGTGCAAAA 
GAGAGTTTAA AGAAGATTCA AGAAATGGAA AGAGATACAA CAGACAGAGC TTGCTCTGAT 
TTAGATGAGC TGAAAAACGC AGACTGGGCT CGGTTTTGGG TGCAGGTCAT GCGGGATCTA 
AGGAACGGTG TCAAGCTAAA GAAAGTGCAA GAGCGGCAGT ACGACCTGTT ACCAATAGAG 
TTTGAGCTGA CACCATATGA GATGCTTATG GATGATATTC GATCTAAACG ATACACCTTA 
AGGAAAGTCA TGGTTCAGGG TGACATCCCA CCCCGATTAA AGAAAAGTGC TCATGAAATA 
ATCCTGGATT TTATACGATC TAGACCTCCA CTTAATCCTG TAGCTGCTCG AAAGCTGAAA 
CCCACCCCAC CCCGTCCACG AAGCCTCCAT GAACAGATCC TGGCAGAGAT CAAGTCAGAG 
CGAAAGCTGC GTCCTGTTTC CCCTGATGAG ATTAGGCGCA GCAGATTAGT AACGCGGCCT 
CTTAGCATGT CTCACAGTTT TGACTTGTCA GATGTATCTA CTCCTGAAGC AACAAGAAAA 
GTGTCTGACA AAACAGTTTT AAATGGTGGG GTGACGCCTA AAGCCAAGGT CAATGGGCAC 
CCGATGCAGG TGGCAGTACA GAGGAAGAAG CTCCTGAAGG CTCCAGCCTT GGCCGAGTTT 
GAGAGTTCAG AATCAGAGGA GGACACAGTG CACAAATCAA CGAGCAGCAG TAGTGTGTCT 
ACTTCCCAGC CAGATGATGT CTCCCCGGAA TCTGCATCCA GCAGGAAATC TCCCCCGAAA 
TTCCTACCCA TATCTTCTAC CCCTCAACCT GAGAGACGTC AAGCTCCTCA AAGACGCCAC 
TCTATTGAGA AGGAGACACC AACCAACGTA CGTCAGTTCA TTCCTCCATC ACGACAGAGC 
TCGAGATCCC TGGAGGAGTT CTGCTACCCG GTGGAATGCC TGGCCCTCAC AGTGGAGGAG 
GTCATGCACA TACGGCAGGT GCTGGTTAAA GCTGAGTTGG AGAAGTTCCA GCAGTATAAA 
GATATCTATA ATGCCCTGAA GAAAGGAAAG CTTTGTTTGT GCTGTCGAAC AAAAAGGTTT 
TCATTATTTA CATGGTCATA CACTTGCCAG TTTTGCAAAA GACCAGTGTG CTCACAATGT 
TGCAAAAAGA TGAGATTACC TTCTAAGCCA TATTCAACAC TTCCCATCTT CTCCCTGGGT 
CCTTCAACCT TGCAGCGAGG GGACAGTTTC CTAAAGCCAG AGAAGCCATC CACCAGTCAC 
CAGCGGCCAC TGAGAAGCTT CCCCAAGATT CCTTCCAAGT CTAAGTCAGT GGAGAGACCA 
GATGAGGAAT TTCAGTTTCC TAAAGAGCTC ATGGAAGACT GGAGCACCAT GGAGGTGTGT 
GTGGACTGTA AAAAGTTTAT CTCGGAAATT ATCAGTTCTA GTCGGCGCAG TTTAACACTC 
GCTAACAAGA GGGCTCGCTT AAAACGGAAG ACGCAATCGT TCTACTTGTC CTCGACAGCA 
GCCTCGGAGT TTCGGCCCAC TGAGAGAACA ATCAATGAGA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_031903694.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759554.1 Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X5, mRNA.

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CloneID	OXa60058
Clone ID Related Accession (Same CDS sequence)	XM_031903694.1
Accession Version	XM_031903694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein spire homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGT CTCAAGCGGC GCCTCGGAAA CGTAGTGAGG CTCAGGGCAG CGGGACTGGC 
GCGGCGGGAG ATGGGGAGTG TTTCTCGCTG GAGGAGATAT TGGATCTATA CAAGCAGCCG 
ATCAATGAGG AGCAAGCTTG GGCTGTGTGC TACCAGTGCT GCGGGGCCCT GCTGGAGGGG 
AGGGGGGGTG GCATTAAGGG AAGGAAGCTA ACTGGAGCCG CCGAGATTTG TATTCGTAAG 
GATGGAGCTG TCATCACCAG GCAGGAGTCG GCGGCAGCAG AGTCGATTGA TTGCAGGTTT 
ACAGAAACAG AGGTTATTGT GTCTCTTGGG ATCATCATTT ACAAAGCGTT GGATTATGGC 
TTGAAGGAGA ATGAGGAAAG AGAGCTTAGC CCTCCTTTGG AGCTACTGAT TGACAACATG 
ACTAACACAG ACATAGCTGG AAATAATGAT GAAGGCTATG ATGCACTGGA TGAAGATGAG 
ACCGACAGTG AAAGAGAATC AGCAGGATTC CGACACTCTT ACAGAAATGT AATGAAGCTT 
TGTACTACTC ATTTGCCAAC CCCATCAGAA GCACCCAATC ACTACCAAGC AGTATGCCGG 
GCCTTGTATG CAGAGACTAT GGAGTTACAC ATGTTCCTGA CCAAAATTAA AAGTGCAAAA 
GAGAGTTTAA AGAAGATTCA AGAAATGGAA AGAGATACAA CAGACAGAGC TTGCTCTGAT 
TTAGATGAGC TGAAAAACGC AGACTGGGCT CGGTTTTGGG TGCAGGTCAT GCGGGATCTA 
AGGAACGGTG TCAAGCTAAA GAAAGTGCAA GAGCGGCAGT ACGACCTGTT ACCAATAGAG 
TTTGAGCTGA CACCATATGA GATGCTTATG GATGATATTC GATCTAAACG ATACACCTTA 
AGGAAAGTCA TGGTTCAGGG TGACATCCCA CCCCGATTAA AGAAAAGTGC TCATGAAATA 
ATCCTGGATT TTATACGATC TAGACCTCCA CTTAATCCTG TAGCTGCTCG AAAGCTGAAA 
CCCACCCCAC CCCGTCCACG AAGCCTCCAT GAACAGATCC TGGCAGAGAT CAAGTCAGAG 
CGAAAGCTGC GTCCTGTTTC CCCTGATGAG ATTAGGCGCA GCAGATTAGA TGTATCTACT 
CCTGAAGCAA CAAGAAAAGT GTCTGACAAA ACAGTTTTAA ATGGTGGGGT GACGCCTAAA 
GCCAAGGTCA ATGGGCACCC GATGCAGGTG GCAGTACAGA GGAAGAAGCT CCTGAAGGCT 
CCAGCCTTGG CCGAGTTTGA GAGTTCAGAA TCAGAGGAGG ACACAGTGCA CAAATCAACG 
AGCAGCAGTA GTGTGTCTAC TTCCCAGCCA GATGATGTCT CCCCGGAATC TGCATCCAGC 
AGGAAATCTC CCCCGAAATT CCTACCCATA TCTTCTACCC CTCAACCTGA GAGACGTCAA 
GCTCCTCAAA GACGCCACTC TATTGAGAAG GAGACACCAA CCAACGTACG TCAGTTCATT 
CCTCCATCAC GACAGAGCTC GAGATCCCTG GAGGAGTTCT GCTACCCGGT GGAATGCCTG 
GCCCTCACAG TGGAGGAGGT CATGCACATA CGGCAGGTGC TGGTTAAAGC TGAGTTGGAG 
AAGTTCCAGC AGTATAAAGA TATCTATAAT GCCCTGAAGA AAGGAAAGCT TTGTTTGTGC 
TGTCGAACAA AAAGGTTTTC ATTATTTACA TGGTCATACA CTTGCCAGTT TTGCAAAAGA 
CCAGTGTGCT CACAATGTTG CAAAAAGATG AGATTACCTT CTAAGCCATA TTCAACACTT 
CCCATCTTCT CCCTGGGTCC TTCAACCTTG CAGCGAGGGG ACAGTTTCCT AAAGCCAGAG 
AAGCCATCCA CCAGTCACCA GCGGCCACTG AGAAGCTTCC CCAAGATTCC TTCCAAGTCT 
AAGTCAGTGG AGAGACCAGA TGAGGAATTT CAGTTTCCTA AAGAGCTCAT GGAAGACTGG 
AGCACCATGG AGGTGTGTGT GGACTGTAAA AAGTTTATCT CGGAAATTAT CAGTTCTAGT 
CGGCGCAGTT TAACACTCGC TAACAAGAGG GCTCGCTTAA AACGGAAGAC GCAATCGTTC 
TACTTGTCCT CGACAGCAGC CTCGGAGTTT CGGCCCACTG AGAGAACAAT CAATGAGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759554.1
CDS	93..2255
Translation	MAESQAAPRKRSEAQGSGTGAAGDGECFSLEEILDLYKQPINEEQAWAVCYQCCGALLEGRGGGIKGRKLTGAAEICIRKDGAVITRQESAAAESIDCRFTETEVIVSLGIIIYKALDYGLKENEERELSPPLELLIDNMTNTDIAGNNDEGYDALDEDETDSERESAGFRHSYRNVMKLCTTHLPTPSEAPNHYQAVCRALYAETMELHMFLTKIKSAKESLKKIQEMERDTTDRACSDLDELKNADWARFWVQVMRDLRNGVKLKKVQERQYDLLPIEFELTPYEMLMDDIRSKRYTLRKVMVQGDIPPRLKKSAHEIILDFIRSRPPLNPVAARKLKPTPPRPRSLHEQILAEIKSERKLRPVSPDEIRRSRLDVSTPEATRKVSDKTVLNGGVTPKAKVNGHPMQVAVQRKKLLKAPALAEFESSESEEDTVHKSTSSSSVSTSQPDDVSPESASSRKSPPKFLPISSTPQPERRQAPQRRHSIEKETPTNVRQFIPPSRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKFQQYKDIYNALKKGKLCLCCRTKRFSLFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSTLQRGDSFLKPEKPSTSHQRPLRSFPKIPSKSKSVERPDEEFQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLTLANKRARLKRKTQSFYLSSTAASEFRPTERTINEI

Target ORF information:

RefSeq Version	XM_031903694.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis spire-type actin nucleation factor 1 (spire1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903694.1

ORF Insert Sequence:

ATGGCCGAGT CTCAAGCGGC GCCTCGGAAA CGTAGTGAGG CTCAGGGCAG CGGGACTGGC 
GCGGCGGGAG ATGGGGAGTG TTTCTCGCTG GAGGAGATAT TGGATCTATA CAAGCAGCCG 
ATCAATGAGG AGCAAGCTTG GGCTGTGTGC TACCAGTGCT GCGGGGCCCT GCTGGAGGGG 
AGGGGGGGTG GCATTAAGGG AAGGAAGCTA ACTGGAGCCG CCGAGATTTG TATTCGTAAG 
GATGGAGCTG TCATCACCAG GCAGGAGTCG GCGGCAGCAG AGTCGATTGA TTGCAGGTTT 
ACAGAAACAG AGGTTATTGT GTCTCTTGGG ATCATCATTT ACAAAGCGTT GGATTATGGC 
TTGAAGGAGA ATGAGGAAAG AGAGCTTAGC CCTCCTTTGG AGCTACTGAT TGACAACATG 
ACTAACACAG ACATAGCTGG AAATAATGAT GAAGGCTATG ATGCACTGGA TGAAGATGAG 
ACCGACAGTG AAAGAGAATC AGCAGGATTC CGACACTCTT ACAGAAATGT AATGAAGCTT 
TGTACTACTC ATTTGCCAAC CCCATCAGAA GCACCCAATC ACTACCAAGC AGTATGCCGG 
GCCTTGTATG CAGAGACTAT GGAGTTACAC ATGTTCCTGA CCAAAATTAA AAGTGCAAAA 
GAGAGTTTAA AGAAGATTCA AGAAATGGAA AGAGATACAA CAGACAGAGC TTGCTCTGAT 
TTAGATGAGC TGAAAAACGC AGACTGGGCT CGGTTTTGGG TGCAGGTCAT GCGGGATCTA 
AGGAACGGTG TCAAGCTAAA GAAAGTGCAA GAGCGGCAGT ACGACCTGTT ACCAATAGAG 
TTTGAGCTGA CACCATATGA GATGCTTATG GATGATATTC GATCTAAACG ATACACCTTA 
AGGAAAGTCA TGGTTCAGGG TGACATCCCA CCCCGATTAA AGAAAAGTGC TCATGAAATA 
ATCCTGGATT TTATACGATC TAGACCTCCA CTTAATCCTG TAGCTGCTCG AAAGCTGAAA 
CCCACCCCAC CCCGTCCACG AAGCCTCCAT GAACAGATCC TGGCAGAGAT CAAGTCAGAG 
CGAAAGCTGC GTCCTGTTTC CCCTGATGAG ATTAGGCGCA GCAGATTAGA TGTATCTACT 
CCTGAAGCAA CAAGAAAAGT GTCTGACAAA ACAGTTTTAA ATGGTGGGGT GACGCCTAAA 
GCCAAGGTCA ATGGGCACCC GATGCAGGTG GCAGTACAGA GGAAGAAGCT CCTGAAGGCT 
CCAGCCTTGG CCGAGTTTGA GAGTTCAGAA TCAGAGGAGG ACACAGTGCA CAAATCAACG 
AGCAGCAGTA GTGTGTCTAC TTCCCAGCCA GATGATGTCT CCCCGGAATC TGCATCCAGC 
AGGAAATCTC CCCCGAAATT CCTACCCATA TCTTCTACCC CTCAACCTGA GAGACGTCAA 
GCTCCTCAAA GACGCCACTC TATTGAGAAG GAGACACCAA CCAACGTACG TCAGTTCATT 
CCTCCATCAC GACAGAGCTC GAGATCCCTG GAGGAGTTCT GCTACCCGGT GGAATGCCTG 
GCCCTCACAG TGGAGGAGGT CATGCACATA CGGCAGGTGC TGGTTAAAGC TGAGTTGGAG 
AAGTTCCAGC AGTATAAAGA TATCTATAAT GCCCTGAAGA AAGGAAAGCT TTGTTTGTGC 
TGTCGAACAA AAAGGTTTTC ATTATTTACA TGGTCATACA CTTGCCAGTT TTGCAAAAGA 
CCAGTGTGCT CACAATGTTG CAAAAAGATG AGATTACCTT CTAAGCCATA TTCAACACTT 
CCCATCTTCT CCCTGGGTCC TTCAACCTTG CAGCGAGGGG ACAGTTTCCT AAAGCCAGAG 
AAGCCATCCA CCAGTCACCA GCGGCCACTG AGAAGCTTCC CCAAGATTCC TTCCAAGTCT 
AAGTCAGTGG AGAGACCAGA TGAGGAATTT CAGTTTCCTA AAGAGCTCAT GGAAGACTGG 
AGCACCATGG AGGTGTGTGT GGACTGTAAA AAGTTTATCT CGGAAATTAT CAGTTCTAGT 
CGGCGCAGTT TAACACTCGC TAACAAGAGG GCTCGCTTAA AACGGAAGAC GCAATCGTTC 
TACTTGTCCT CGACAGCAGC CTCGGAGTTT CGGCCCACTG AGAGAACAAT CAATGAGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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spire1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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