gfm2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	gfm2
Entrez Gene ID	100492326
Full Name	G elongation factor, mitochondrial 2
General protein information	Preferred Names G elongation factor, mitochondrial 2 Names ribosome-releasing factor 2, mitochondrial
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002934840.4	XP_002934886.2	ribosome-releasing factor 2, mitochondrial isoform X2
XM_004910397.3	XP_004910454.1	ribosome-releasing factor 2, mitochondrial isoform X1
XM_004910397.2	XP_004910454.1	ribosome-releasing factor 2, mitochondrial isoform X1
XM_002934840.3	XP_002934886.2	ribosome-releasing factor 2, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GFM2	XP_005617535.1
Gallus gallus (chicken)	GFM2	XP_004937415.1
Caenorhabditis elegans (roundworm)	Y119D3B.14	NP_497345.2
Kluyveromyces lactis	KLLA0F13992g	XP_455710.1
Schizosaccharomyces pombe (fission yeast)	SPBC660.10	NP_595089.1
Xenopus tropicalis (tropical clawed frog)	gfm2	XP_004910454.1
Bos taurus (cattle)	GFM2	NP_001095579.1
Saccharomyces cerevisiae (baker's yeast)	MEF2	NP_012433.1
Magnaporthe oryzae (rice blast fungus)	MGG_01433	XP_003714382.1
Homo sapiens (human)	GFM2	NP_001268231.1
Mus musculus (house mouse)	Gfm2	NP_796240.3
Rattus norvegicus (Norway rat)	Gfm2	NP_001094135.1
Drosophila melanogaster (fruit fly)	EF-G2	NP_732771.2
Eremothecium gossypii	AGOS_AFR085W	NP_985632.1
	GFM2	XP_001102249.2
Danio rerio (zebrafish)	gfm2	NP_001071013.1
Anopheles gambiae (African malaria mosquito)	RRF2M_ANOGA	XP_317594.4
Neurospora crassa	NCU07021	XP_962160.2
Pan troglodytes (chimpanzee)	GFM2	XP_001150063.1

Gene Ontology

The following gfm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gfm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03356	XM_004910397.3 Latest version!	Xenopus tropicalis G elongation factor, mitochondrial 2 (gfm2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa03357	XM_002934840.4 Latest version!	Xenopus tropicalis G elongation factor, mitochondrial 2 (gfm2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa03356	XM_004910397.2	Xenopus tropicalis G elongation factor, mitochondrial 2 (gfm2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa03357	XM_002934840.3	Xenopus tropicalis G elongation factor, mitochondrial 2 (gfm2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gfm2 ( XM_004910397.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910454.1 Xenopus tropicalis G elongation factor, mitochondrial 2 (gfm2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa03356
Clone ID Related Accession (Same CDS sequence)	XM_004910397.3 , XM_004910397.2
Accession Version	XM_004910397.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ribosome-releasing factor 2, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004910397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAAAG TACTAAACCT ATTACCTGGG AAGCTTTCCA CCATAAGCTG CCTACATACA 
GAGTGCCTGC AGTGCTGTAG AAGAAGAGCT TTTCAGAATA GAAAAATAAA ATATTATATT 
CACCATCACC GACAGTTTAA CTTATCAGCA GGAGATGTCA AATCACTGCG GACCATCATA 
AACCCTCCAG TATCCAAGAT CAGAAATATT GGTATCATGG CACATATCGA TGCAGGCAAG 
ACAACTACCA CTGAAAGAAT GCTTTACTAC TCTGGATATA TCAGGGCACT GGGAGATGTT 
GATGATGGGG ATACAGTGAC TGATTATATG GCGCAAGAAA GGGAGCGGGG AATCACCATT 
CAGTCAGCTG CTGTTACATT TGACTGGAAC GGTCACAGGA TAAACCTGAT TGACACGCCA 
GGGCATGTGG ATTTCACTGT TGAGGTAGAA CGATCCCTGA GGGTGCTTGA TGGGGCAGTT 
GCTGTATTTG ATGCTTCAGC TGGAGTGGAG GCTCAGACAC TGACAGTGTG GAGACAAGCG 
GACAGACACA CCATCCCACG CCTGTGTTTC ATAAACAAGA TGGACAAAAC TGGAGCCAGT 
TTTAATTATG CAGTGGAAAG CATCAAGGAA AAGTTAAAAG TAAAGCCGTT GGTGTTACAG 
TTACCAGTGG GCGAGGGCAA ATCATTCAAA GGACTGGTGG ACCTGGTCAG CATGGAAACA 
ATTCTGTGGA ATCCGAGAGC CCAGGAGGAT GATGGGAGAC ACTTTGAGAA GAAGGCTGTA 
AAGGAAATAA GTGATGTAGA AATACTGGAC ATTGCCACTG ATGGAAGGAA TGCTTTAATA 
GAACAAGTTG CAGATCTGGA TGATGAATTT GCTGCCTTGA TTCTAGGTGA ATACAGTGAC 
CATTTTGATT TAGTGCCGCT TGAAAAGTTA CAGATGGCAA TACGGCGCAT CACTCTAGCA 
CAGTTGGCGG TCCCCATCCT GTGTGGAAGT GCTTTAAAGA ATAAAGGTGT TCAGCCTCTG 
CTGGATGCAA TTACATTATA CTTGCCTTCT CCAGAAGATT TTTCTCATGA TTTTGTAAAG 
TGGTATCAAG GGGACTTGTG TGCATTGGCA TTTAAAGTTA TGCATGACAA GCAGCGGGGG 
CCCATGGTGT TTGTGCGGAT ATATTCGGGA AACATGAAAC CACAATCTGC ACTATATAAT 
GTGAACCAAA ACTGCACAGA GAGGATGAGC CGTTTGCTGC TGCCATTTGC TGACCAACAT 
GTTGAAATTC CTTCACTGTC ATCAGGCAAC ATTGCTCTAA CTGTTGGCCT GAAGCAGGCA 
GCTACAGGGG ATACCATTGT CTCCTCTAAG GCGGCGGCAG CGGCTGCTGC ACGCAGAGCA 
GGCCGGGAGG GGGACAAGAA GCACAGAGAT GGGAAGACAG AGGGAAGCCT TGTTCTGGCT 
GGTGTGGAGA TACCAGAACC TGTCTTTTTT TGTACCATTG AGCCTCCCTC TGCAGCCAGA 
CAGCCAGATT TGGAAAATGC TTTAAGGTGT CTCCAGCGGG AAGATCCAAG CCTGAAAGTA 
AAGGTGGATG CTGACTCTGG CCAGATGATC CTGTGTGGGA TGGGCGAGTT GCACATCGAG 
ATTATCCATG AGCGCATCAG GCAGGAGTAT GGACTAGAGA CCTATCTTGG ACCATTACAG 
ATTGCATACA GGGAAACCAT CCTGAGTGCT TCTAGTGCTT CAGATACCCT GGACAGAACA 
GTGGGGGATA TGCGGCACTT GGTTACAGTA GAACTGGAAG TGAAACCCTG TGCCACTGAC 
GGCCATGGAC CAAACCCAGT TATTGAATAT GCTAAGGAGA TTTCTGAGCC GCTACTGAAA 
GACTTTCAAG AAGCAGTGGA AAATGGCATC AGCAGCTCAT ATCTGCAAGG TCCCCTGCTG 
GGTTCCCCCT TGCAAGATGT TGGGGTTACT GTAAATTCAG TCAGTGTGCA TCCGGGTACC 
TCTGTTACAA TGGTGTCTGC CTGTGTTTCC CGGTGTATGA GTAAGGCACT GAAGAAAGCA 
GAGAAACAAG TGCTGGAGCC TCTGATGAAG CTGGAGATCA CTGTGAGCGA GGAGCATCTG 
AGCTGTGCGC TGGGGGACCT TGCACAAAGG AGGGGCAACA TTCAGGAAAT ACTGAGTCGC 
TCAGATAACA AAGTTGTGGT TGCGTATGTT CCTTTAGCAG AAATGATGGG TTACTCTACA 
GATCTGCGCT CCTTGACATC AGGCTCAGCG ACATTTACCC TCGAGCTGTC TAATTATGAA 
GCAATGAACA TGCAAGATCA GAACGCTTTA CTCAAGAAAA AGGGCTTTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910454.1
CDS	231..2564
Translation	MLKVLNLLPGKLSTISCLHTECLQCCRRRAFQNRKIKYYIHHHRQFNLSAGDVKSLRTIINPPVSKIRNIGIMAHIDAGKTTTTERMLYYSGYIRALGDVDDGDTVTDYMAQERERGITIQSAAVTFDWNGHRINLIDTPGHVDFTVEVERSLRVLDGAVAVFDASAGVEAQTLTVWRQADRHTIPRLCFINKMDKTGASFNYAVESIKEKLKVKPLVLQLPVGEGKSFKGLVDLVSMETILWNPRAQEDDGRHFEKKAVKEISDVEILDIATDGRNALIEQVADLDDEFAALILGEYSDHFDLVPLEKLQMAIRRITLAQLAVPILCGSALKNKGVQPLLDAITLYLPSPEDFSHDFVKWYQGDLCALAFKVMHDKQRGPMVFVRIYSGNMKPQSALYNVNQNCTERMSRLLLPFADQHVEIPSLSSGNIALTVGLKQAATGDTIVSSKAAAAAAARRAGREGDKKHRDGKTEGSLVLAGVEIPEPVFFCTIEPPSAARQPDLENALRCLQREDPSLKVKVDADSGQMILCGMGELHIEIIHERIRQEYGLETYLGPLQIAYRETILSASSASDTLDRTVGDMRHLVTVELEVKPCATDGHGPNPVIEYAKEISEPLLKDFQEAVENGISSSYLQGPLLGSPLQDVGVTVNSVSVHPGTSVTMVSACVSRCMSKALKKAEKQVLEPLMKLEITVSEEHLSCALGDLAQRRGNIQEILSRSDNKVVVAYVPLAEMMGYSTDLRSLTSGSATFTLELSNYEAMNMQDQNALLKKKGFA

Target ORF information:

RefSeq Version	XM_004910397.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis G elongation factor, mitochondrial 2 (gfm2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910397.2

ORF Insert Sequence:

ATGTTAAAAG TACTAAACCT ATTACCTGGG AAGCTTTCCA CCATAAGCTG CCTACATACA 
GAGTGCCTGC AGTGCTGTAG AAGAAGAGCT TTTCAGAATA GAAAAATAAA ATATTATATT 
CACCATCACC GACAGTTTAA CTTATCAGCA GGAGATGTCA AATCACTGCG GACCATCATA 
AACCCTCCAG TATCCAAGAT CAGAAATATT GGTATCATGG CACATATCGA TGCAGGCAAG 
ACAACTACCA CTGAAAGAAT GCTTTACTAC TCTGGATATA TCAGGGCACT GGGAGATGTT 
GATGATGGGG ATACAGTGAC TGATTATATG GCGCAAGAAA GGGAGCGGGG AATCACCATT 
CAGTCAGCTG CTGTTACATT TGACTGGAAC GGTCACAGGA TAAACCTGAT TGACACGCCA 
GGGCATGTGG ATTTCACTGT TGAGGTAGAA CGATCCCTGA GGGTGCTTGA TGGGGCAGTT 
GCTGTATTTG ATGCTTCAGC TGGAGTGGAG GCTCAGACAC TGACAGTGTG GAGACAAGCG 
GACAGACACA CCATCCCACG CCTGTGTTTC ATAAACAAGA TGGACAAAAC TGGAGCCAGT 
TTTAATTATG CAGTGGAAAG CATCAAGGAA AAGTTAAAAG TAAAGCCGTT GGTGTTACAG 
TTACCAGTGG GCGAGGGCAA ATCATTCAAA GGACTGGTGG ACCTGGTCAG CATGGAAACA 
ATTCTGTGGA ATCCGAGAGC CCAGGAGGAT GATGGGAGAC ACTTTGAGAA GAAGGCTGTA 
AAGGAAATAA GTGATGTAGA AATACTGGAC ATTGCCACTG ATGGAAGGAA TGCTTTAATA 
GAACAAGTTG CAGATCTGGA TGATGAATTT GCTGCCTTGA TTCTAGGTGA ATACAGTGAC 
CATTTTGATT TAGTGCCGCT TGAAAAGTTA CAGATGGCAA TACGGCGCAT CACTCTAGCA 
CAGTTGGCGG TCCCCATCCT GTGTGGAAGT GCTTTAAAGA ATAAAGGTGT TCAGCCTCTG 
CTGGATGCAA TTACATTATA CTTGCCTTCT CCAGAAGATT TTTCTCATGA TTTTGTAAAG 
TGGTATCAAG GGGACTTGTG TGCATTGGCA TTTAAAGTTA TGCATGACAA GCAGCGGGGG 
CCCATGGTGT TTGTGCGGAT ATATTCGGGA AACATGAAAC CACAATCTGC ACTATATAAT 
GTGAACCAAA ACTGCACAGA GAGGATGAGC CGTTTGCTGC TGCCATTTGC TGACCAACAT 
GTTGAAATTC CTTCACTGTC ATCAGGCAAC ATTGCTCTAA CTGTTGGCCT GAAGCAGGCA 
GCTACAGGGG ATACCATTGT CTCCTCTAAG GCGGCGGCAG CGGCTGCTGC ACGCAGAGCA 
GGCCGGGAGG GGGACAAGAA GCACAGAGAT GGGAAGACAG AGGGAAGCCT TGTTCTGGCT 
GGTGTGGAGA TACCAGAACC TGTCTTTTTT TGTACCATTG AGCCTCCCTC TGCAGCCAGA 
CAGCCAGATT TGGAAAATGC TTTAAGGTGT CTCCAGCGGG AAGATCCAAG CCTGAAAGTA 
AAGGTGGATG CTGACTCTGG CCAGATGATC CTGTGTGGGA TGGGCGAGTT GCACATCGAG 
ATTATCCATG AGCGCATCAG GCAGGAGTAT GGACTAGAGA CCTATCTTGG ACCATTACAG 
ATTGCATACA GGGAAACCAT CCTGAGTGCT TCTAGTGCTT CAGATACCCT GGACAGAACA 
GTGGGGGATA TGCGGCACTT GGTTACAGTA GAACTGGAAG TGAAACCCTG TGCCACTGAC 
GGCCATGGAC CAAACCCAGT TATTGAATAT GCTAAGGAGA TTTCTGAGCC GCTACTGAAA 
GACTTTCAAG AAGCAGTGGA AAATGGCATC AGCAGCTCAT ATCTGCAAGG TCCCCTGCTG 
GGTTCCCCCT TGCAAGATGT TGGGGTTACT GTAAATTCAG TCAGTGTGCA TCCGGGTACC 
TCTGTTACAA TGGTGTCTGC CTGTGTTTCC CGGTGTATGA GTAAGGCACT GAAGAAAGCA 
GAGAAACAAG TGCTGGAGCC TCTGATGAAG CTGGAGATCA CTGTGAGCGA GGAGCATCTG 
AGCTGTGCGC TGGGGGACCT TGCACAAAGG AGGGGCAACA TTCAGGAAAT ACTGAGTCGC 
TCAGATAACA AAGTTGTGGT TGCGTATGTT CCTTTAGCAG AAATGATGGG TTACTCTACA 
GATCTGCGCT CCTTGACATC AGGCTCAGCG ACATTTACCC TCGAGCTGTC TAATTATGAA 
GCAATGAACA TGCAAGATCA GAACGCTTTA CTCAAGAAAA AGGGCTTTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gfm2 ( XM_002934840.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934886.2 Xenopus tropicalis G elongation factor, mitochondrial 2 (gfm2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa03357
Clone ID Related Accession (Same CDS sequence)	XM_002934840.4 , XM_002934840.3
Accession Version	XM_002934840.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ribosome-releasing factor 2, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002934840.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGAA ATATTGGTAT CATGGCACAT ATCGATGCAG GCAAGACAAC TACCACTGAA 
AGAATGCTTT ACTACTCTGG ATATATCAGG GCACTGGGAG ATGTTGATGA TGGGGATACA 
GTGACTGATT ATATGGCGCA AGAAAGGGAG CGGGGAATCA CCATTCAGTC AGCTGCTGTT 
ACATTTGACT GGAACGGTCA CAGGATAAAC CTGATTGACA CGCCAGGGCA TGTGGATTTC 
ACTGTTGAGG TAGAACGATC CCTGAGGGTG CTTGATGGGG CAGTTGCTGT ATTTGATGCT 
TCAGCTGGAG TGGAGGCTCA GACACTGACA GTGTGGAGAC AAGCGGACAG ACACACCATC 
CCACGCCTGT GTTTCATAAA CAAGATGGAC AAAACTGGAG CCAGTTTTAA TTATGCAGTG 
GAAAGCATCA AGGAAAAGTT AAAAGTAAAG CCGTTGGTGT TACAGTTACC AGTGGGCGAG 
GGCAAATCAT TCAAAGGACT GGTGGACCTG GTCAGCATGG AAACAATTCT GTGGAATCCG 
AGAGCCCAGG AGGATGATGG GAGACACTTT GAGAAGAAGG CTGTAAAGGA AATAAGTGAT 
GTAGAAATAC TGGACATTGC CACTGATGGA AGGAATGCTT TAATAGAACA AGTTGCAGAT 
CTGGATGATG AATTTGCTGC CTTGATTCTA GGTGAATACA GTGACCATTT TGATTTAGTG 
CCGCTTGAAA AGTTACAGAT GGCAATACGG CGCATCACTC TAGCACAGTT GGCGGTCCCC 
ATCCTGTGTG GAAGTGCTTT AAAGAATAAA GGTGTTCAGC CTCTGCTGGA TGCAATTACA 
TTATACTTGC CTTCTCCAGA AGATTTTTCT CATGATTTTG TAAAGTGGTA TCAAGGGGAC 
TTGTGTGCAT TGGCATTTAA AGTTATGCAT GACAAGCAGC GGGGGCCCAT GGTGTTTGTG 
CGGATATATT CGGGAAACAT GAAACCACAA TCTGCACTAT ATAATGTGAA CCAAAACTGC 
ACAGAGAGGA TGAGCCGTTT GCTGCTGCCA TTTGCTGACC AACATGTTGA AATTCCTTCA 
CTGTCATCAG GCAACATTGC TCTAACTGTT GGCCTGAAGC AGGCAGCTAC AGGGGATACC 
ATTGTCTCCT CTAAGGCGGC GGCAGCGGCT GCTGCACGCA GAGCAGGCCG GGAGGGGGAC 
AAGAAGCACA GAGATGGGAA GACAGAGGGA AGCCTTGTTC TGGCTGGTGT GGAGATACCA 
GAACCTGTCT TTTTTTGTAC CATTGAGCCT CCCTCTGCAG CCAGACAGCC AGATTTGGAA 
AATGCTTTAA GGTGTCTCCA GCGGGAAGAT CCAAGCCTGA AAGTAAAGGT GGATGCTGAC 
TCTGGCCAGA TGATCCTGTG TGGGATGGGC GAGTTGCACA TCGAGATTAT CCATGAGCGC 
ATCAGGCAGG AGTATGGACT AGAGACCTAT CTTGGACCAT TACAGATTGC ATACAGGGAA 
ACCATCCTGA GTGCTTCTAG TGCTTCAGAT ACCCTGGACA GAACAGTGGG GGATATGCGG 
CACTTGGTTA CAGTAGAACT GGAAGTGAAA CCCTGTGCCA CTGACGGCCA TGGACCAAAC 
CCAGTTATTG AATATGCTAA GGAGATTTCT GAGCCGCTAC TGAAAGACTT TCAAGAAGCA 
GTGGAAAATG GCATCAGCAG CTCATATCTG CAAGGTCCCC TGCTGGGTTC CCCCTTGCAA 
GATGTTGGGG TTACTGTAAA TTCAGTCAGT GTGCATCCGG GTACCTCTGT TACAATGGTG 
TCTGCCTGTG TTTCCCGGTG TATGAGTAAG GCACTGAAGA AAGCAGAGAA ACAAGTGCTG 
GAGCCTCTGA TGAAGCTGGA GATCACTGTG AGCGAGGAGC ATCTGAGCTG TGCGCTGGGG 
GACCTTGCAC AAAGGAGGGG CAACATTCAG GAAATACTGA GTCGCTCAGA TAACAAAGTT 
GTGGTTGCGT ATGTTCCTTT AGCAGAAATG ATGGGTTACT CTACAGATCT GCGCTCCTTG 
ACATCAGGCT CAGCGACATT TACCCTCGAG CTGTCTAATT ATGAAGCAAT GAACATGCAA 
GATCAGAACG CTTTACTCAA GAAAAAGGGC TTTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934886.2
CDS	51..2189
Translation	MIRNIGIMAHIDAGKTTTTERMLYYSGYIRALGDVDDGDTVTDYMAQERERGITIQSAAVTFDWNGHRINLIDTPGHVDFTVEVERSLRVLDGAVAVFDASAGVEAQTLTVWRQADRHTIPRLCFINKMDKTGASFNYAVESIKEKLKVKPLVLQLPVGEGKSFKGLVDLVSMETILWNPRAQEDDGRHFEKKAVKEISDVEILDIATDGRNALIEQVADLDDEFAALILGEYSDHFDLVPLEKLQMAIRRITLAQLAVPILCGSALKNKGVQPLLDAITLYLPSPEDFSHDFVKWYQGDLCALAFKVMHDKQRGPMVFVRIYSGNMKPQSALYNVNQNCTERMSRLLLPFADQHVEIPSLSSGNIALTVGLKQAATGDTIVSSKAAAAAAARRAGREGDKKHRDGKTEGSLVLAGVEIPEPVFFCTIEPPSAARQPDLENALRCLQREDPSLKVKVDADSGQMILCGMGELHIEIIHERIRQEYGLETYLGPLQIAYRETILSASSASDTLDRTVGDMRHLVTVELEVKPCATDGHGPNPVIEYAKEISEPLLKDFQEAVENGISSSYLQGPLLGSPLQDVGVTVNSVSVHPGTSVTMVSACVSRCMSKALKKAEKQVLEPLMKLEITVSEEHLSCALGDLAQRRGNIQEILSRSDNKVVVAYVPLAEMMGYSTDLRSLTSGSATFTLELSNYEAMNMQDQNALLKKKGFA

Target ORF information:

RefSeq Version	XM_002934840.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis G elongation factor, mitochondrial 2 (gfm2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934840.3

ORF Insert Sequence:

ATGATCAGAA ATATTGGTAT CATGGCACAT ATCGATGCAG GCAAGACAAC TACCACTGAA 
AGAATGCTTT ACTACTCTGG ATATATCAGG GCACTGGGAG ATGTTGATGA TGGGGATACA 
GTGACTGATT ATATGGCGCA AGAAAGGGAG CGGGGAATCA CCATTCAGTC AGCTGCTGTT 
ACATTTGACT GGAACGGTCA CAGGATAAAC CTGATTGACA CGCCAGGGCA TGTGGATTTC 
ACTGTTGAGG TAGAACGATC CCTGAGGGTG CTTGATGGGG CAGTTGCTGT ATTTGATGCT 
TCAGCTGGAG TGGAGGCTCA GACACTGACA GTGTGGAGAC AAGCGGACAG ACACACCATC 
CCACGCCTGT GTTTCATAAA CAAGATGGAC AAAACTGGAG CCAGTTTTAA TTATGCAGTG 
GAAAGCATCA AGGAAAAGTT AAAAGTAAAG CCGTTGGTGT TACAGTTACC AGTGGGCGAG 
GGCAAATCAT TCAAAGGACT GGTGGACCTG GTCAGCATGG AAACAATTCT GTGGAATCCG 
AGAGCCCAGG AGGATGATGG GAGACACTTT GAGAAGAAGG CTGTAAAGGA AATAAGTGAT 
GTAGAAATAC TGGACATTGC CACTGATGGA AGGAATGCTT TAATAGAACA AGTTGCAGAT 
CTGGATGATG AATTTGCTGC CTTGATTCTA GGTGAATACA GTGACCATTT TGATTTAGTG 
CCGCTTGAAA AGTTACAGAT GGCAATACGG CGCATCACTC TAGCACAGTT GGCGGTCCCC 
ATCCTGTGTG GAAGTGCTTT AAAGAATAAA GGTGTTCAGC CTCTGCTGGA TGCAATTACA 
TTATACTTGC CTTCTCCAGA AGATTTTTCT CATGATTTTG TAAAGTGGTA TCAAGGGGAC 
TTGTGTGCAT TGGCATTTAA AGTTATGCAT GACAAGCAGC GGGGGCCCAT GGTGTTTGTG 
CGGATATATT CGGGAAACAT GAAACCACAA TCTGCACTAT ATAATGTGAA CCAAAACTGC 
ACAGAGAGGA TGAGCCGTTT GCTGCTGCCA TTTGCTGACC AACATGTTGA AATTCCTTCA 
CTGTCATCAG GCAACATTGC TCTAACTGTT GGCCTGAAGC AGGCAGCTAC AGGGGATACC 
ATTGTCTCCT CTAAGGCGGC GGCAGCGGCT GCTGCACGCA GAGCAGGCCG GGAGGGGGAC 
AAGAAGCACA GAGATGGGAA GACAGAGGGA AGCCTTGTTC TGGCTGGTGT GGAGATACCA 
GAACCTGTCT TTTTTTGTAC CATTGAGCCT CCCTCTGCAG CCAGACAGCC AGATTTGGAA 
AATGCTTTAA GGTGTCTCCA GCGGGAAGAT CCAAGCCTGA AAGTAAAGGT GGATGCTGAC 
TCTGGCCAGA TGATCCTGTG TGGGATGGGC GAGTTGCACA TCGAGATTAT CCATGAGCGC 
ATCAGGCAGG AGTATGGACT AGAGACCTAT CTTGGACCAT TACAGATTGC ATACAGGGAA 
ACCATCCTGA GTGCTTCTAG TGCTTCAGAT ACCCTGGACA GAACAGTGGG GGATATGCGG 
CACTTGGTTA CAGTAGAACT GGAAGTGAAA CCCTGTGCCA CTGACGGCCA TGGACCAAAC 
CCAGTTATTG AATATGCTAA GGAGATTTCT GAGCCGCTAC TGAAAGACTT TCAAGAAGCA 
GTGGAAAATG GCATCAGCAG CTCATATCTG CAAGGTCCCC TGCTGGGTTC CCCCTTGCAA 
GATGTTGGGG TTACTGTAAA TTCAGTCAGT GTGCATCCGG GTACCTCTGT TACAATGGTG 
TCTGCCTGTG TTTCCCGGTG TATGAGTAAG GCACTGAAGA AAGCAGAGAA ACAAGTGCTG 
GAGCCTCTGA TGAAGCTGGA GATCACTGTG AGCGAGGAGC ATCTGAGCTG TGCGCTGGGG 
GACCTTGCAC AAAGGAGGGG CAACATTCAG GAAATACTGA GTCGCTCAGA TAACAAAGTT 
GTGGTTGCGT ATGTTCCTTT AGCAGAAATG ATGGGTTACT CTACAGATCT GCGCTCCTTG 
ACATCAGGCT CAGCGACATT TACCCTCGAG CTGTCTAATT ATGAAGCAAT GAACATGCAA 
GATCAGAACG CTTTACTCAA GAAAAAGGGC TTTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gfm2 ( XM_004910397.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910454.1 Xenopus tropicalis G elongation factor, mitochondrial 2 (gfm2), transcript variant X1, mRNA.

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CloneID	OXa03356
Clone ID Related Accession (Same CDS sequence)	XM_004910397.3 , XM_004910397.2
Accession Version	XM_004910397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ribosome-releasing factor 2, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAAAG TACTAAACCT ATTACCTGGG AAGCTTTCCA CCATAAGCTG CCTACATACA 
GAGTGCCTGC AGTGCTGTAG AAGAAGAGCT TTTCAGAATA GAAAAATAAA ATATTATATT 
CACCATCACC GACAGTTTAA CTTATCAGCA GGAGATGTCA AATCACTGCG GACCATCATA 
AACCCTCCAG TATCCAAGAT CAGAAATATT GGTATCATGG CACATATCGA TGCAGGCAAG 
ACAACTACCA CTGAAAGAAT GCTTTACTAC TCTGGATATA TCAGGGCACT GGGAGATGTT 
GATGATGGGG ATACAGTGAC TGATTATATG GCGCAAGAAA GGGAGCGGGG AATCACCATT 
CAGTCAGCTG CTGTTACATT TGACTGGAAC GGTCACAGGA TAAACCTGAT TGACACGCCA 
GGGCATGTGG ATTTCACTGT TGAGGTAGAA CGATCCCTGA GGGTGCTTGA TGGGGCAGTT 
GCTGTATTTG ATGCTTCAGC TGGAGTGGAG GCTCAGACAC TGACAGTGTG GAGACAAGCG 
GACAGACACA CCATCCCACG CCTGTGTTTC ATAAACAAGA TGGACAAAAC TGGAGCCAGT 
TTTAATTATG CAGTGGAAAG CATCAAGGAA AAGTTAAAAG TAAAGCCGTT GGTGTTACAG 
TTACCAGTGG GCGAGGGCAA ATCATTCAAA GGACTGGTGG ACCTGGTCAG CATGGAAACA 
ATTCTGTGGA ATCCGAGAGC CCAGGAGGAT GATGGGAGAC ACTTTGAGAA GAAGGCTGTA 
AAGGAAATAA GTGATGTAGA AATACTGGAC ATTGCCACTG ATGGAAGGAA TGCTTTAATA 
GAACAAGTTG CAGATCTGGA TGATGAATTT GCTGCCTTGA TTCTAGGTGA ATACAGTGAC 
CATTTTGATT TAGTGCCGCT TGAAAAGTTA CAGATGGCAA TACGGCGCAT CACTCTAGCA 
CAGTTGGCGG TCCCCATCCT GTGTGGAAGT GCTTTAAAGA ATAAAGGTGT TCAGCCTCTG 
CTGGATGCAA TTACATTATA CTTGCCTTCT CCAGAAGATT TTTCTCATGA TTTTGTAAAG 
TGGTATCAAG GGGACTTGTG TGCATTGGCA TTTAAAGTTA TGCATGACAA GCAGCGGGGG 
CCCATGGTGT TTGTGCGGAT ATATTCGGGA AACATGAAAC CACAATCTGC ACTATATAAT 
GTGAACCAAA ACTGCACAGA GAGGATGAGC CGTTTGCTGC TGCCATTTGC TGACCAACAT 
GTTGAAATTC CTTCACTGTC ATCAGGCAAC ATTGCTCTAA CTGTTGGCCT GAAGCAGGCA 
GCTACAGGGG ATACCATTGT CTCCTCTAAG GCGGCGGCAG CGGCTGCTGC ACGCAGAGCA 
GGCCGGGAGG GGGACAAGAA GCACAGAGAT GGGAAGACAG AGGGAAGCCT TGTTCTGGCT 
GGTGTGGAGA TACCAGAACC TGTCTTTTTT TGTACCATTG AGCCTCCCTC TGCAGCCAGA 
CAGCCAGATT TGGAAAATGC TTTAAGGTGT CTCCAGCGGG AAGATCCAAG CCTGAAAGTA 
AAGGTGGATG CTGACTCTGG CCAGATGATC CTGTGTGGGA TGGGCGAGTT GCACATCGAG 
ATTATCCATG AGCGCATCAG GCAGGAGTAT GGACTAGAGA CCTATCTTGG ACCATTACAG 
ATTGCATACA GGGAAACCAT CCTGAGTGCT TCTAGTGCTT CAGATACCCT GGACAGAACA 
GTGGGGGATA TGCGGCACTT GGTTACAGTA GAACTGGAAG TGAAACCCTG TGCCACTGAC 
GGCCATGGAC CAAACCCAGT TATTGAATAT GCTAAGGAGA TTTCTGAGCC GCTACTGAAA 
GACTTTCAAG AAGCAGTGGA AAATGGCATC AGCAGCTCAT ATCTGCAAGG TCCCCTGCTG 
GGTTCCCCCT TGCAAGATGT TGGGGTTACT GTAAATTCAG TCAGTGTGCA TCCGGGTACC 
TCTGTTACAA TGGTGTCTGC CTGTGTTTCC CGGTGTATGA GTAAGGCACT GAAGAAAGCA 
GAGAAACAAG TGCTGGAGCC TCTGATGAAG CTGGAGATCA CTGTGAGCGA GGAGCATCTG 
AGCTGTGCGC TGGGGGACCT TGCACAAAGG AGGGGCAACA TTCAGGAAAT ACTGAGTCGC 
TCAGATAACA AAGTTGTGGT TGCGTATGTT CCTTTAGCAG AAATGATGGG TTACTCTACA 
GATCTGCGCT CCTTGACATC AGGCTCAGCG ACATTTACCC TCGAGCTGTC TAATTATGAA 
GCAATGAACA TGCAAGATCA GAACGCTTTA CTCAAGAAAA AGGGCTTTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910454.1
CDS	215..2548
Translation	MLKVLNLLPGKLSTISCLHTECLQCCRRRAFQNRKIKYYIHHHRQFNLSAGDVKSLRTIINPPVSKIRNIGIMAHIDAGKTTTTERMLYYSGYIRALGDVDDGDTVTDYMAQERERGITIQSAAVTFDWNGHRINLIDTPGHVDFTVEVERSLRVLDGAVAVFDASAGVEAQTLTVWRQADRHTIPRLCFINKMDKTGASFNYAVESIKEKLKVKPLVLQLPVGEGKSFKGLVDLVSMETILWNPRAQEDDGRHFEKKAVKEISDVEILDIATDGRNALIEQVADLDDEFAALILGEYSDHFDLVPLEKLQMAIRRITLAQLAVPILCGSALKNKGVQPLLDAITLYLPSPEDFSHDFVKWYQGDLCALAFKVMHDKQRGPMVFVRIYSGNMKPQSALYNVNQNCTERMSRLLLPFADQHVEIPSLSSGNIALTVGLKQAATGDTIVSSKAAAAAAARRAGREGDKKHRDGKTEGSLVLAGVEIPEPVFFCTIEPPSAARQPDLENALRCLQREDPSLKVKVDADSGQMILCGMGELHIEIIHERIRQEYGLETYLGPLQIAYRETILSASSASDTLDRTVGDMRHLVTVELEVKPCATDGHGPNPVIEYAKEISEPLLKDFQEAVENGISSSYLQGPLLGSPLQDVGVTVNSVSVHPGTSVTMVSACVSRCMSKALKKAEKQVLEPLMKLEITVSEEHLSCALGDLAQRRGNIQEILSRSDNKVVVAYVPLAEMMGYSTDLRSLTSGSATFTLELSNYEAMNMQDQNALLKKKGFA

Target ORF information:

RefSeq Version	XM_004910397.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis G elongation factor, mitochondrial 2 (gfm2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910397.3

ORF Insert Sequence:

ATGTTAAAAG TACTAAACCT ATTACCTGGG AAGCTTTCCA CCATAAGCTG CCTACATACA 
GAGTGCCTGC AGTGCTGTAG AAGAAGAGCT TTTCAGAATA GAAAAATAAA ATATTATATT 
CACCATCACC GACAGTTTAA CTTATCAGCA GGAGATGTCA AATCACTGCG GACCATCATA 
AACCCTCCAG TATCCAAGAT CAGAAATATT GGTATCATGG CACATATCGA TGCAGGCAAG 
ACAACTACCA CTGAAAGAAT GCTTTACTAC TCTGGATATA TCAGGGCACT GGGAGATGTT 
GATGATGGGG ATACAGTGAC TGATTATATG GCGCAAGAAA GGGAGCGGGG AATCACCATT 
CAGTCAGCTG CTGTTACATT TGACTGGAAC GGTCACAGGA TAAACCTGAT TGACACGCCA 
GGGCATGTGG ATTTCACTGT TGAGGTAGAA CGATCCCTGA GGGTGCTTGA TGGGGCAGTT 
GCTGTATTTG ATGCTTCAGC TGGAGTGGAG GCTCAGACAC TGACAGTGTG GAGACAAGCG 
GACAGACACA CCATCCCACG CCTGTGTTTC ATAAACAAGA TGGACAAAAC TGGAGCCAGT 
TTTAATTATG CAGTGGAAAG CATCAAGGAA AAGTTAAAAG TAAAGCCGTT GGTGTTACAG 
TTACCAGTGG GCGAGGGCAA ATCATTCAAA GGACTGGTGG ACCTGGTCAG CATGGAAACA 
ATTCTGTGGA ATCCGAGAGC CCAGGAGGAT GATGGGAGAC ACTTTGAGAA GAAGGCTGTA 
AAGGAAATAA GTGATGTAGA AATACTGGAC ATTGCCACTG ATGGAAGGAA TGCTTTAATA 
GAACAAGTTG CAGATCTGGA TGATGAATTT GCTGCCTTGA TTCTAGGTGA ATACAGTGAC 
CATTTTGATT TAGTGCCGCT TGAAAAGTTA CAGATGGCAA TACGGCGCAT CACTCTAGCA 
CAGTTGGCGG TCCCCATCCT GTGTGGAAGT GCTTTAAAGA ATAAAGGTGT TCAGCCTCTG 
CTGGATGCAA TTACATTATA CTTGCCTTCT CCAGAAGATT TTTCTCATGA TTTTGTAAAG 
TGGTATCAAG GGGACTTGTG TGCATTGGCA TTTAAAGTTA TGCATGACAA GCAGCGGGGG 
CCCATGGTGT TTGTGCGGAT ATATTCGGGA AACATGAAAC CACAATCTGC ACTATATAAT 
GTGAACCAAA ACTGCACAGA GAGGATGAGC CGTTTGCTGC TGCCATTTGC TGACCAACAT 
GTTGAAATTC CTTCACTGTC ATCAGGCAAC ATTGCTCTAA CTGTTGGCCT GAAGCAGGCA 
GCTACAGGGG ATACCATTGT CTCCTCTAAG GCGGCGGCAG CGGCTGCTGC ACGCAGAGCA 
GGCCGGGAGG GGGACAAGAA GCACAGAGAT GGGAAGACAG AGGGAAGCCT TGTTCTGGCT 
GGTGTGGAGA TACCAGAACC TGTCTTTTTT TGTACCATTG AGCCTCCCTC TGCAGCCAGA 
CAGCCAGATT TGGAAAATGC TTTAAGGTGT CTCCAGCGGG AAGATCCAAG CCTGAAAGTA 
AAGGTGGATG CTGACTCTGG CCAGATGATC CTGTGTGGGA TGGGCGAGTT GCACATCGAG 
ATTATCCATG AGCGCATCAG GCAGGAGTAT GGACTAGAGA CCTATCTTGG ACCATTACAG 
ATTGCATACA GGGAAACCAT CCTGAGTGCT TCTAGTGCTT CAGATACCCT GGACAGAACA 
GTGGGGGATA TGCGGCACTT GGTTACAGTA GAACTGGAAG TGAAACCCTG TGCCACTGAC 
GGCCATGGAC CAAACCCAGT TATTGAATAT GCTAAGGAGA TTTCTGAGCC GCTACTGAAA 
GACTTTCAAG AAGCAGTGGA AAATGGCATC AGCAGCTCAT ATCTGCAAGG TCCCCTGCTG 
GGTTCCCCCT TGCAAGATGT TGGGGTTACT GTAAATTCAG TCAGTGTGCA TCCGGGTACC 
TCTGTTACAA TGGTGTCTGC CTGTGTTTCC CGGTGTATGA GTAAGGCACT GAAGAAAGCA 
GAGAAACAAG TGCTGGAGCC TCTGATGAAG CTGGAGATCA CTGTGAGCGA GGAGCATCTG 
AGCTGTGCGC TGGGGGACCT TGCACAAAGG AGGGGCAACA TTCAGGAAAT ACTGAGTCGC 
TCAGATAACA AAGTTGTGGT TGCGTATGTT CCTTTAGCAG AAATGATGGG TTACTCTACA 
GATCTGCGCT CCTTGACATC AGGCTCAGCG ACATTTACCC TCGAGCTGTC TAATTATGAA 
GCAATGAACA TGCAAGATCA GAACGCTTTA CTCAAGAAAA AGGGCTTTGC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gfm2 ( XM_002934840.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934886.2 Xenopus tropicalis G elongation factor, mitochondrial 2 (gfm2), transcript variant X2, mRNA.

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CloneID	OXa03357
Clone ID Related Accession (Same CDS sequence)	XM_002934840.4 , XM_002934840.3
Accession Version	XM_002934840.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ribosome-releasing factor 2, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934840.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGAA ATATTGGTAT CATGGCACAT ATCGATGCAG GCAAGACAAC TACCACTGAA 
AGAATGCTTT ACTACTCTGG ATATATCAGG GCACTGGGAG ATGTTGATGA TGGGGATACA 
GTGACTGATT ATATGGCGCA AGAAAGGGAG CGGGGAATCA CCATTCAGTC AGCTGCTGTT 
ACATTTGACT GGAACGGTCA CAGGATAAAC CTGATTGACA CGCCAGGGCA TGTGGATTTC 
ACTGTTGAGG TAGAACGATC CCTGAGGGTG CTTGATGGGG CAGTTGCTGT ATTTGATGCT 
TCAGCTGGAG TGGAGGCTCA GACACTGACA GTGTGGAGAC AAGCGGACAG ACACACCATC 
CCACGCCTGT GTTTCATAAA CAAGATGGAC AAAACTGGAG CCAGTTTTAA TTATGCAGTG 
GAAAGCATCA AGGAAAAGTT AAAAGTAAAG CCGTTGGTGT TACAGTTACC AGTGGGCGAG 
GGCAAATCAT TCAAAGGACT GGTGGACCTG GTCAGCATGG AAACAATTCT GTGGAATCCG 
AGAGCCCAGG AGGATGATGG GAGACACTTT GAGAAGAAGG CTGTAAAGGA AATAAGTGAT 
GTAGAAATAC TGGACATTGC CACTGATGGA AGGAATGCTT TAATAGAACA AGTTGCAGAT 
CTGGATGATG AATTTGCTGC CTTGATTCTA GGTGAATACA GTGACCATTT TGATTTAGTG 
CCGCTTGAAA AGTTACAGAT GGCAATACGG CGCATCACTC TAGCACAGTT GGCGGTCCCC 
ATCCTGTGTG GAAGTGCTTT AAAGAATAAA GGTGTTCAGC CTCTGCTGGA TGCAATTACA 
TTATACTTGC CTTCTCCAGA AGATTTTTCT CATGATTTTG TAAAGTGGTA TCAAGGGGAC 
TTGTGTGCAT TGGCATTTAA AGTTATGCAT GACAAGCAGC GGGGGCCCAT GGTGTTTGTG 
CGGATATATT CGGGAAACAT GAAACCACAA TCTGCACTAT ATAATGTGAA CCAAAACTGC 
ACAGAGAGGA TGAGCCGTTT GCTGCTGCCA TTTGCTGACC AACATGTTGA AATTCCTTCA 
CTGTCATCAG GCAACATTGC TCTAACTGTT GGCCTGAAGC AGGCAGCTAC AGGGGATACC 
ATTGTCTCCT CTAAGGCGGC GGCAGCGGCT GCTGCACGCA GAGCAGGCCG GGAGGGGGAC 
AAGAAGCACA GAGATGGGAA GACAGAGGGA AGCCTTGTTC TGGCTGGTGT GGAGATACCA 
GAACCTGTCT TTTTTTGTAC CATTGAGCCT CCCTCTGCAG CCAGACAGCC AGATTTGGAA 
AATGCTTTAA GGTGTCTCCA GCGGGAAGAT CCAAGCCTGA AAGTAAAGGT GGATGCTGAC 
TCTGGCCAGA TGATCCTGTG TGGGATGGGC GAGTTGCACA TCGAGATTAT CCATGAGCGC 
ATCAGGCAGG AGTATGGACT AGAGACCTAT CTTGGACCAT TACAGATTGC ATACAGGGAA 
ACCATCCTGA GTGCTTCTAG TGCTTCAGAT ACCCTGGACA GAACAGTGGG GGATATGCGG 
CACTTGGTTA CAGTAGAACT GGAAGTGAAA CCCTGTGCCA CTGACGGCCA TGGACCAAAC 
CCAGTTATTG AATATGCTAA GGAGATTTCT GAGCCGCTAC TGAAAGACTT TCAAGAAGCA 
GTGGAAAATG GCATCAGCAG CTCATATCTG CAAGGTCCCC TGCTGGGTTC CCCCTTGCAA 
GATGTTGGGG TTACTGTAAA TTCAGTCAGT GTGCATCCGG GTACCTCTGT TACAATGGTG 
TCTGCCTGTG TTTCCCGGTG TATGAGTAAG GCACTGAAGA AAGCAGAGAA ACAAGTGCTG 
GAGCCTCTGA TGAAGCTGGA GATCACTGTG AGCGAGGAGC ATCTGAGCTG TGCGCTGGGG 
GACCTTGCAC AAAGGAGGGG CAACATTCAG GAAATACTGA GTCGCTCAGA TAACAAAGTT 
GTGGTTGCGT ATGTTCCTTT AGCAGAAATG ATGGGTTACT CTACAGATCT GCGCTCCTTG 
ACATCAGGCT CAGCGACATT TACCCTCGAG CTGTCTAATT ATGAAGCAAT GAACATGCAA 
GATCAGAACG CTTTACTCAA GAAAAAGGGC TTTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934886.2
CDS	76..2214
Translation	MIRNIGIMAHIDAGKTTTTERMLYYSGYIRALGDVDDGDTVTDYMAQERERGITIQSAAVTFDWNGHRINLIDTPGHVDFTVEVERSLRVLDGAVAVFDASAGVEAQTLTVWRQADRHTIPRLCFINKMDKTGASFNYAVESIKEKLKVKPLVLQLPVGEGKSFKGLVDLVSMETILWNPRAQEDDGRHFEKKAVKEISDVEILDIATDGRNALIEQVADLDDEFAALILGEYSDHFDLVPLEKLQMAIRRITLAQLAVPILCGSALKNKGVQPLLDAITLYLPSPEDFSHDFVKWYQGDLCALAFKVMHDKQRGPMVFVRIYSGNMKPQSALYNVNQNCTERMSRLLLPFADQHVEIPSLSSGNIALTVGLKQAATGDTIVSSKAAAAAAARRAGREGDKKHRDGKTEGSLVLAGVEIPEPVFFCTIEPPSAARQPDLENALRCLQREDPSLKVKVDADSGQMILCGMGELHIEIIHERIRQEYGLETYLGPLQIAYRETILSASSASDTLDRTVGDMRHLVTVELEVKPCATDGHGPNPVIEYAKEISEPLLKDFQEAVENGISSSYLQGPLLGSPLQDVGVTVNSVSVHPGTSVTMVSACVSRCMSKALKKAEKQVLEPLMKLEITVSEEHLSCALGDLAQRRGNIQEILSRSDNKVVVAYVPLAEMMGYSTDLRSLTSGSATFTLELSNYEAMNMQDQNALLKKKGFA

Target ORF information:

RefSeq Version	XM_002934840.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis G elongation factor, mitochondrial 2 (gfm2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934840.4

ORF Insert Sequence:

ATGATCAGAA ATATTGGTAT CATGGCACAT ATCGATGCAG GCAAGACAAC TACCACTGAA 
AGAATGCTTT ACTACTCTGG ATATATCAGG GCACTGGGAG ATGTTGATGA TGGGGATACA 
GTGACTGATT ATATGGCGCA AGAAAGGGAG CGGGGAATCA CCATTCAGTC AGCTGCTGTT 
ACATTTGACT GGAACGGTCA CAGGATAAAC CTGATTGACA CGCCAGGGCA TGTGGATTTC 
ACTGTTGAGG TAGAACGATC CCTGAGGGTG CTTGATGGGG CAGTTGCTGT ATTTGATGCT 
TCAGCTGGAG TGGAGGCTCA GACACTGACA GTGTGGAGAC AAGCGGACAG ACACACCATC 
CCACGCCTGT GTTTCATAAA CAAGATGGAC AAAACTGGAG CCAGTTTTAA TTATGCAGTG 
GAAAGCATCA AGGAAAAGTT AAAAGTAAAG CCGTTGGTGT TACAGTTACC AGTGGGCGAG 
GGCAAATCAT TCAAAGGACT GGTGGACCTG GTCAGCATGG AAACAATTCT GTGGAATCCG 
AGAGCCCAGG AGGATGATGG GAGACACTTT GAGAAGAAGG CTGTAAAGGA AATAAGTGAT 
GTAGAAATAC TGGACATTGC CACTGATGGA AGGAATGCTT TAATAGAACA AGTTGCAGAT 
CTGGATGATG AATTTGCTGC CTTGATTCTA GGTGAATACA GTGACCATTT TGATTTAGTG 
CCGCTTGAAA AGTTACAGAT GGCAATACGG CGCATCACTC TAGCACAGTT GGCGGTCCCC 
ATCCTGTGTG GAAGTGCTTT AAAGAATAAA GGTGTTCAGC CTCTGCTGGA TGCAATTACA 
TTATACTTGC CTTCTCCAGA AGATTTTTCT CATGATTTTG TAAAGTGGTA TCAAGGGGAC 
TTGTGTGCAT TGGCATTTAA AGTTATGCAT GACAAGCAGC GGGGGCCCAT GGTGTTTGTG 
CGGATATATT CGGGAAACAT GAAACCACAA TCTGCACTAT ATAATGTGAA CCAAAACTGC 
ACAGAGAGGA TGAGCCGTTT GCTGCTGCCA TTTGCTGACC AACATGTTGA AATTCCTTCA 
CTGTCATCAG GCAACATTGC TCTAACTGTT GGCCTGAAGC AGGCAGCTAC AGGGGATACC 
ATTGTCTCCT CTAAGGCGGC GGCAGCGGCT GCTGCACGCA GAGCAGGCCG GGAGGGGGAC 
AAGAAGCACA GAGATGGGAA GACAGAGGGA AGCCTTGTTC TGGCTGGTGT GGAGATACCA 
GAACCTGTCT TTTTTTGTAC CATTGAGCCT CCCTCTGCAG CCAGACAGCC AGATTTGGAA 
AATGCTTTAA GGTGTCTCCA GCGGGAAGAT CCAAGCCTGA AAGTAAAGGT GGATGCTGAC 
TCTGGCCAGA TGATCCTGTG TGGGATGGGC GAGTTGCACA TCGAGATTAT CCATGAGCGC 
ATCAGGCAGG AGTATGGACT AGAGACCTAT CTTGGACCAT TACAGATTGC ATACAGGGAA 
ACCATCCTGA GTGCTTCTAG TGCTTCAGAT ACCCTGGACA GAACAGTGGG GGATATGCGG 
CACTTGGTTA CAGTAGAACT GGAAGTGAAA CCCTGTGCCA CTGACGGCCA TGGACCAAAC 
CCAGTTATTG AATATGCTAA GGAGATTTCT GAGCCGCTAC TGAAAGACTT TCAAGAAGCA 
GTGGAAAATG GCATCAGCAG CTCATATCTG CAAGGTCCCC TGCTGGGTTC CCCCTTGCAA 
GATGTTGGGG TTACTGTAAA TTCAGTCAGT GTGCATCCGG GTACCTCTGT TACAATGGTG 
TCTGCCTGTG TTTCCCGGTG TATGAGTAAG GCACTGAAGA AAGCAGAGAA ACAAGTGCTG 
GAGCCTCTGA TGAAGCTGGA GATCACTGTG AGCGAGGAGC ATCTGAGCTG TGCGCTGGGG 
GACCTTGCAC AAAGGAGGGG CAACATTCAG GAAATACTGA GTCGCTCAGA TAACAAAGTT 
GTGGTTGCGT ATGTTCCTTT AGCAGAAATG ATGGGTTACT CTACAGATCT GCGCTCCTTG 
ACATCAGGCT CAGCGACATT TACCCTCGAG CTGTCTAATT ATGAAGCAAT GAACATGCAA 
GATCAGAACG CTTTACTCAA GAAAAAGGGC TTTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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gfm2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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