aars cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	aars
Entrez Gene ID	100492308
Full Name	alanyl-tRNA synthetase
General protein information	Preferred Names alanyl-tRNA synthetase Names alanine--tRNA ligase, cytoplasmic
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004914051.4	XP_004914108.2	alanine--tRNA ligase, cytoplasmic isoform X2
XM_018093363.2	XP_017948852.2	alanine--tRNA ligase, cytoplasmic isoform X1
XM_002931661.5	XP_002931707.2	alanine--tRNA ligase, cytoplasmic isoform X2
XM_012962061.2	XP_012817515.1	alanine--tRNA ligase, cytoplasmic isoform X2
XM_004914051.3	XP_004914108.1	alanine--tRNA ligase, cytoplasmic isoform X3
XM_018093363.1	XP_017948852.1	alanine--tRNA ligase, cytoplasmic isoform X1
XM_002931661.4	XP_002931707.1	alanine--tRNA ligase, cytoplasmic isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AARS	XP_001169520.2
Caenorhabditis elegans (roundworm)	aars-2	NP_491281.1
Neurospora crassa	NCU02566	XP_965697.2
Xenopus tropicalis (tropical clawed frog)	aars	XP_002931707.1
	AARS	XP_002808351.1
Mus musculus (house mouse)	Aars	NP_666329.2
Danio rerio (zebrafish)	aars	NP_001037775.1
Oryza sativa (rice)	Os10g0182000	NP_001064254.1
Canis lupus familiaris (dog)	AARS	XP_536788.2
Gallus gallus (chicken)	AARS	NP_001005836.1
Drosophila melanogaster (fruit fly)	Aats-ala	NP_523511.2
Kluyveromyces lactis	KLLA0F02431g	XP_455190.1
Schizosaccharomyces pombe (fission yeast)	ala1	NP_593640.1
Homo sapiens (human)	AARS	NP_001596.2
Bos taurus (cattle)	AARS	NP_001094545.1
Rattus norvegicus (Norway rat)	Aars	NP_001093987.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009701	XP_318757.4
Saccharomyces cerevisiae (baker's yeast)	ALA1	NP_014980.3
Eremothecium gossypii	AGOS_ADR363C	NP_984459.1
Magnaporthe oryzae (rice blast fungus)	MGG_03607	XP_003716291.1
Arabidopsis thaliana (thale cress)	ALATS	NP_001185186.1

Gene Ontology

The following aars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa09591	XM_002931661.4 Latest version!	Xenopus tropicalis alanyl-tRNA synthetase (aars), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa09591	XM_004914051.3 Latest version!	Xenopus tropicalis alanyl-tRNA synthetase (aars), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa35355	XM_012962061.2 Latest version!	Xenopus tropicalis alanyl-tRNA synthetase (aars), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa44032	XM_018093363.1 Latest version!	Xenopus tropicalis alanyl-tRNA synthetase (aars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa56966	XM_018093363.2 Latest version!	Xenopus tropicalis alanyl-tRNA synthetase 1 (aars1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa56967	XM_004914051.4 Latest version!	Xenopus tropicalis alanyl-tRNA synthetase 1 (aars1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa56967	XM_002931661.5 Latest version!	Xenopus tropicalis alanyl-tRNA synthetase 1 (aars1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aars ( XM_004914051.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914108.1 Xenopus tropicalis alanyl-tRNA synthetase (aars), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa09591
Clone ID Related Accession (Same CDS sequence)	XM_004914051.3 , XM_002931661.4
Accession Version	XM_004914051.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alanine--tRNA ligase, cytoplasmic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914051.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGT GTATGACAGC CTCTGAAATC CGGCAGAAGT TTATTGACTT CTTTAAAGAA 
AATGCCCACA CATATGTCCA TTCTTCTGCC ACAGTCCCCC TGGATGACCC AACACTGCTC 
TTCGCCAATG CTGGAATGAA CCAGTTTAAG CCAATCTTCC TGAATACCAT TGATCCTTCT 
CATCCAATGG CAAAGCTAAA CCGAGCAGCT AATACACAGA AGTGCATCCG TGCTGGAGGC 
AAGCACAATG ATCTCGATGA TGTGGGCAAA GATGTCTATC ACCACACATT TTTTGAGATG 
CTTGGATCAT GGTCCTTTGG GGATTATTTC AAGGAGCTCG CCTGTAAACT GGCACTGGAC 
CTGCTAACCA AGGAGTTTGG CATTGACATA GAGCGCTTGT ATGTCACTTA TTTTGGCGGT 
AATGAGGCTG CTGGTCTAGA GCCTGACTTG GAATGTAAAC AAATATGGCT GAACTTGGGC 
CTGCCAGAGA GTCGTATCCT TCCAGGAAGC ATGAAGGACA ACTTTTGGGA AATGGGTGAT 
ACGGGTCCTT GTGGTCCCTG TAGTGAGATT CATTTTGACC GAATTGGGGG CCGTGATGCT 
GCCCATCTTG TGAATATGGA TGATCCAAAT GTCCTTGAGA TCTGGAACCT GGTGTTCATC 
CAGTTTAATA GGGAAACTGA TGGAACCTTA AAGCCACTGC CTAAGAAGAG CATTGATACT 
GGAATGGGAC TGGAAAGACT TGTGTCTGTG TTGCAAAATA AGATGTCCAA CTATGATACA 
GATCTGTTTG TTCCATACTT TGAAGCTATC GAAAAGGGCA CAGGTGCAAG ACCCTACACT 
GGGAAAGTGG GAGCTGAGGA TGTTGATGGC ATAGACATGG CATACAGAGT GTTGGCTGAT 
CATGCTAGAA CAATTACTGT GGCACTTGCT GATGGAGGAA GACCAGATAA CACTGGACGT 
GGATATGTTT TACGCCGCAT CCTTCGCCGT GCTGTGAGAT ACGCCCATGA GAAACTTAAT 
GCATCTAAAG GATTTTTTGC CACTCTGGTT GATGTGGTTG TCCAGTCACT GGGAAATGCT 
TTTCCAGAGC TTAAAAAAGA TCCTGATATG GTCAAAGACA TCATTAATGA AGAGGAAACA 
CAGTTCCTGA AAACACTTAA CAGGGGTCGT CGCATCTTGG ACCGAAAGAT AAACAGTCTG 
GGAGAACACA AAGTTATCCC TGGTGACACT GCCTGGCTGC TGTACGACAC CTACGGCTTC 
CCAGTGGATT TGACTAGTTT GATTGCTGAG GAACGAGGAC TGACATTGGA CTTGGAGAGC 
TTTGAAGAGG AGCGTAAAGC TGCTCAGCTT AAATCTCAAG GAAAGGGTAC AGGTGACGAA 
GACCTTCTCA TGCTGGACAT CTATGCAATT GAAGAACTGC GTTCCCAGGG ACTTGAACCC 
ACTGATGATC TGCCAAAATA CAAATATACA TCTAATGCTG ATGGTGTTTA CGAATTTGAG 
AGTTTGATAG CAACAGTGAA AGCAATCCGC AGAGATAAGA TGTTTGTGGA AGAGGCGAGC 
ACTGGACAGG AGTGTGGGCT GGTGCTTGAC AAGACCTGTT TTTATGCAGA ACAGGGAGGG 
CAGACCTATG ATGAGGGCTA CATGGTTAAA GAGGAGGAGA GCAGTGAAGA TAAAACAGAG 
TTTACAGTGA GAAACACTCA GGTTCGGGGA GGTTACGTGC TGCACATGGG TACATTGTAC 
GGGCACTTGA AAGTTGGAGA TCAAGTGTGT CTATACGTTG ATGAGCCCAA GCGTCGTCCT 
ATTATGAGTA ACCACACAGC CACTCACATC CTGAACTTTG CCTTGCGCTC TGTTCTTGGA 
GAAGCAGATC AACGGGGTTC ATTGGTTGCC CCAGACAGGC TAAGATTTGA CTTCACAGCT 
AAAGGTGCAA TGTCCACCCA GGAGATCCGC AAAACCGAAG AGATTGCAAA TGACATTATC 
AAGGAAAATA AGGTTGTGTA TGCACTAGAT TGCTCTCTCG CAGAAGCAAA AGCTATCCAG 
GGTCTTCGTG CAGTATTTGA TGAAACATAC CCAGATCCTG TGCGTGTTGT GTCTATAGGA 
ATTCCTGTGG AAGATCTGCT GGCTGACCCA TCTAGTCCTG CTGGGTCTAT AACTTCTGTG 
GAGTTCTGTG GAGGAACGCA TCTCCAAAAT TCTGGCCATG CTGGTCCTTT CATTATTGTT 
ACAGAGGAAG CCATTGCCAA AGGAATTCGC AGAATTGTTG CTGTCACAGG TGCAGAGGCA 
CAAAAGGCCT TAAGGAGACA GGATGCCCTG AAAGTTAATC TTTCTGATCT GGAGAAGAAA 
GTGAAGAAAC AGAATTCTCC TAACAAGGAT GTACAAAAGG AAATAGCTGA CCTTAGTGAG 
AATCTCGCAA CAGCATCCAT TGCACAGTGG CAGAAGGATG TACTAAGGGA GCAGCTGAAA 
ACCTTGAAGA AAACTATGGA TGACCTGGAT CGTGCCAGCA AAGCAGATGT TCAGAAACGG 
GTATTGGAAA TAACCAAACA ACTCATAGAG GAACATCCTA ATCAGCCACT ACTTGTACTT 
GAGATGGAGA ATGGAGCATC TGCAAAGGCT TTGAACGAGT CCCTGAAACT CCTCAAGTCT 
CAGTCTCCCC ATACTGCTGC AATGATCTTC TCTGTAGATA ACGATGCAGG AAAGATCACG 
TGTTTATGCC AGGTGCCCCA GGACACTGCA GACAAAGGTC TGAAGGCCAA TGAGTGGGTG 
CAGCAAGTAT CTGATTTGAT GGATGGCAGG GGAGGAGGAA AAGACACCTC TGCCCAAGCA 
ACTGGAAAGA ATGTGGGGTG CCTGTCCCAA GTCCTAAAGC TAGCTGAAGA TTTTGCCAAA 
ATGAAACTGG AGTCCATGAA AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914108.1
CDS	108..3014
Translation	MKTCMTASEIRQKFIDFFKENAHTYVHSSATVPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLNRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKLALDLLTKEFGIDIERLYVTYFGGNEAAGLEPDLECKQIWLNLGLPESRILPGSMKDNFWEMGDTGPCGPCSEIHFDRIGGRDAAHLVNMDDPNVLEIWNLVFIQFNRETDGTLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIEKGTGARPYTGKVGAEDVDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASKGFFATLVDVVVQSLGNAFPELKKDPDMVKDIINEEETQFLKTLNRGRRILDRKINSLGEHKVIPGDTAWLLYDTYGFPVDLTSLIAEERGLTLDLESFEEERKAAQLKSQGKGTGDEDLLMLDIYAIEELRSQGLEPTDDLPKYKYTSNADGVYEFESLIATVKAIRRDKMFVEEASTGQECGLVLDKTCFYAEQGGQTYDEGYMVKEEESSEDKTEFTVRNTQVRGGYVLHMGTLYGHLKVGDQVCLYVDEPKRRPIMSNHTATHILNFALRSVLGEADQRGSLVAPDRLRFDFTAKGAMSTQEIRKTEEIANDIIKENKVVYALDCSLAEAKAIQGLRAVFDETYPDPVRVVSIGIPVEDLLADPSSPAGSITSVEFCGGTHLQNSGHAGPFIIVTEEAIAKGIRRIVAVTGAEAQKALRRQDALKVNLSDLEKKVKKQNSPNKDVQKEIADLSENLATASIAQWQKDVLREQLKTLKKTMDDLDRASKADVQKRVLEITKQLIEEHPNQPLLVLEMENGASAKALNESLKLLKSQSPHTAAMIFSVDNDAGKITCLCQVPQDTADKGLKANEWVQQVSDLMDGRGGGKDTSAQATGKNVGCLSQVLKLAEDFAKMKLESMKN

Target ORF information:

RefSeq Version	XM_004914051.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis alanyl-tRNA synthetase (aars), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914051.3

ORF Insert Sequence:

ATGAAGACGT GTATGACAGC CTCTGAAATC CGGCAGAAGT TTATTGACTT CTTTAAAGAA 
AATGCCCACA CATATGTCCA TTCTTCTGCC ACAGTCCCCC TGGATGACCC AACACTGCTC 
TTCGCCAATG CTGGAATGAA CCAGTTTAAG CCAATCTTCC TGAATACCAT TGATCCTTCT 
CATCCAATGG CAAAGCTAAA CCGAGCAGCT AATACACAGA AGTGCATCCG TGCTGGAGGC 
AAGCACAATG ATCTCGATGA TGTGGGCAAA GATGTCTATC ACCACACATT TTTTGAGATG 
CTTGGATCAT GGTCCTTTGG GGATTATTTC AAGGAGCTCG CCTGTAAACT GGCACTGGAC 
CTGCTAACCA AGGAGTTTGG CATTGACATA GAGCGCTTGT ATGTCACTTA TTTTGGCGGT 
AATGAGGCTG CTGGTCTAGA GCCTGACTTG GAATGTAAAC AAATATGGCT GAACTTGGGC 
CTGCCAGAGA GTCGTATCCT TCCAGGAAGC ATGAAGGACA ACTTTTGGGA AATGGGTGAT 
ACGGGTCCTT GTGGTCCCTG TAGTGAGATT CATTTTGACC GAATTGGGGG CCGTGATGCT 
GCCCATCTTG TGAATATGGA TGATCCAAAT GTCCTTGAGA TCTGGAACCT GGTGTTCATC 
CAGTTTAATA GGGAAACTGA TGGAACCTTA AAGCCACTGC CTAAGAAGAG CATTGATACT 
GGAATGGGAC TGGAAAGACT TGTGTCTGTG TTGCAAAATA AGATGTCCAA CTATGATACA 
GATCTGTTTG TTCCATACTT TGAAGCTATC GAAAAGGGCA CAGGTGCAAG ACCCTACACT 
GGGAAAGTGG GAGCTGAGGA TGTTGATGGC ATAGACATGG CATACAGAGT GTTGGCTGAT 
CATGCTAGAA CAATTACTGT GGCACTTGCT GATGGAGGAA GACCAGATAA CACTGGACGT 
GGATATGTTT TACGCCGCAT CCTTCGCCGT GCTGTGAGAT ACGCCCATGA GAAACTTAAT 
GCATCTAAAG GATTTTTTGC CACTCTGGTT GATGTGGTTG TCCAGTCACT GGGAAATGCT 
TTTCCAGAGC TTAAAAAAGA TCCTGATATG GTCAAAGACA TCATTAATGA AGAGGAAACA 
CAGTTCCTGA AAACACTTAA CAGGGGTCGT CGCATCTTGG ACCGAAAGAT AAACAGTCTG 
GGAGAACACA AAGTTATCCC TGGTGACACT GCCTGGCTGC TGTACGACAC CTACGGCTTC 
CCAGTGGATT TGACTAGTTT GATTGCTGAG GAACGAGGAC TGACATTGGA CTTGGAGAGC 
TTTGAAGAGG AGCGTAAAGC TGCTCAGCTT AAATCTCAAG GAAAGGGTAC AGGTGACGAA 
GACCTTCTCA TGCTGGACAT CTATGCAATT GAAGAACTGC GTTCCCAGGG ACTTGAACCC 
ACTGATGATC TGCCAAAATA CAAATATACA TCTAATGCTG ATGGTGTTTA CGAATTTGAG 
AGTTTGATAG CAACAGTGAA AGCAATCCGC AGAGATAAGA TGTTTGTGGA AGAGGCGAGC 
ACTGGACAGG AGTGTGGGCT GGTGCTTGAC AAGACCTGTT TTTATGCAGA ACAGGGAGGG 
CAGACCTATG ATGAGGGCTA CATGGTTAAA GAGGAGGAGA GCAGTGAAGA TAAAACAGAG 
TTTACAGTGA GAAACACTCA GGTTCGGGGA GGTTACGTGC TGCACATGGG TACATTGTAC 
GGGCACTTGA AAGTTGGAGA TCAAGTGTGT CTATACGTTG ATGAGCCCAA GCGTCGTCCT 
ATTATGAGTA ACCACACAGC CACTCACATC CTGAACTTTG CCTTGCGCTC TGTTCTTGGA 
GAAGCAGATC AACGGGGTTC ATTGGTTGCC CCAGACAGGC TAAGATTTGA CTTCACAGCT 
AAAGGTGCAA TGTCCACCCA GGAGATCCGC AAAACCGAAG AGATTGCAAA TGACATTATC 
AAGGAAAATA AGGTTGTGTA TGCACTAGAT TGCTCTCTCG CAGAAGCAAA AGCTATCCAG 
GGTCTTCGTG CAGTATTTGA TGAAACATAC CCAGATCCTG TGCGTGTTGT GTCTATAGGA 
ATTCCTGTGG AAGATCTGCT GGCTGACCCA TCTAGTCCTG CTGGGTCTAT AACTTCTGTG 
GAGTTCTGTG GAGGAACGCA TCTCCAAAAT TCTGGCCATG CTGGTCCTTT CATTATTGTT 
ACAGAGGAAG CCATTGCCAA AGGAATTCGC AGAATTGTTG CTGTCACAGG TGCAGAGGCA 
CAAAAGGCCT TAAGGAGACA GGATGCCCTG AAAGTTAATC TTTCTGATCT GGAGAAGAAA 
GTGAAGAAAC AGAATTCTCC TAACAAGGAT GTACAAAAGG AAATAGCTGA CCTTAGTGAG 
AATCTCGCAA CAGCATCCAT TGCACAGTGG CAGAAGGATG TACTAAGGGA GCAGCTGAAA 
ACCTTGAAGA AAACTATGGA TGACCTGGAT CGTGCCAGCA AAGCAGATGT TCAGAAACGG 
GTATTGGAAA TAACCAAACA ACTCATAGAG GAACATCCTA ATCAGCCACT ACTTGTACTT 
GAGATGGAGA ATGGAGCATC TGCAAAGGCT TTGAACGAGT CCCTGAAACT CCTCAAGTCT 
CAGTCTCCCC ATACTGCTGC AATGATCTTC TCTGTAGATA ACGATGCAGG AAAGATCACG 
TGTTTATGCC AGGTGCCCCA GGACACTGCA GACAAAGGTC TGAAGGCCAA TGAGTGGGTG 
CAGCAAGTAT CTGATTTGAT GGATGGCAGG GGAGGAGGAA AAGACACCTC TGCCCAAGCA 
ACTGGAAAGA ATGTGGGGTG CCTGTCCCAA GTCCTAAAGC TAGCTGAAGA TTTTGCCAAA 
ATGAAACTGG AGTCCATGAA AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aars ( XM_002931661.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931707.1 Xenopus tropicalis alanyl-tRNA synthetase (aars), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa09591
Clone ID Related Accession (Same CDS sequence)	XM_004914051.3 , XM_002931661.4
Accession Version	XM_002931661.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alanine--tRNA ligase, cytoplasmic isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931661.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGT GTATGACAGC CTCTGAAATC CGGCAGAAGT TTATTGACTT CTTTAAAGAA 
AATGCCCACA CATATGTCCA TTCTTCTGCC ACAGTCCCCC TGGATGACCC AACACTGCTC 
TTCGCCAATG CTGGAATGAA CCAGTTTAAG CCAATCTTCC TGAATACCAT TGATCCTTCT 
CATCCAATGG CAAAGCTAAA CCGAGCAGCT AATACACAGA AGTGCATCCG TGCTGGAGGC 
AAGCACAATG ATCTCGATGA TGTGGGCAAA GATGTCTATC ACCACACATT TTTTGAGATG 
CTTGGATCAT GGTCCTTTGG GGATTATTTC AAGGAGCTCG CCTGTAAACT GGCACTGGAC 
CTGCTAACCA AGGAGTTTGG CATTGACATA GAGCGCTTGT ATGTCACTTA TTTTGGCGGT 
AATGAGGCTG CTGGTCTAGA GCCTGACTTG GAATGTAAAC AAATATGGCT GAACTTGGGC 
CTGCCAGAGA GTCGTATCCT TCCAGGAAGC ATGAAGGACA ACTTTTGGGA AATGGGTGAT 
ACGGGTCCTT GTGGTCCCTG TAGTGAGATT CATTTTGACC GAATTGGGGG CCGTGATGCT 
GCCCATCTTG TGAATATGGA TGATCCAAAT GTCCTTGAGA TCTGGAACCT GGTGTTCATC 
CAGTTTAATA GGGAAACTGA TGGAACCTTA AAGCCACTGC CTAAGAAGAG CATTGATACT 
GGAATGGGAC TGGAAAGACT TGTGTCTGTG TTGCAAAATA AGATGTCCAA CTATGATACA 
GATCTGTTTG TTCCATACTT TGAAGCTATC GAAAAGGGCA CAGGTGCAAG ACCCTACACT 
GGGAAAGTGG GAGCTGAGGA TGTTGATGGC ATAGACATGG CATACAGAGT GTTGGCTGAT 
CATGCTAGAA CAATTACTGT GGCACTTGCT GATGGAGGAA GACCAGATAA CACTGGACGT 
GGATATGTTT TACGCCGCAT CCTTCGCCGT GCTGTGAGAT ACGCCCATGA GAAACTTAAT 
GCATCTAAAG GATTTTTTGC CACTCTGGTT GATGTGGTTG TCCAGTCACT GGGAAATGCT 
TTTCCAGAGC TTAAAAAAGA TCCTGATATG GTCAAAGACA TCATTAATGA AGAGGAAACA 
CAGTTCCTGA AAACACTTAA CAGGGGTCGT CGCATCTTGG ACCGAAAGAT AAACAGTCTG 
GGAGAACACA AAGTTATCCC TGGTGACACT GCCTGGCTGC TGTACGACAC CTACGGCTTC 
CCAGTGGATT TGACTAGTTT GATTGCTGAG GAACGAGGAC TGACATTGGA CTTGGAGAGC 
TTTGAAGAGG AGCGTAAAGC TGCTCAGCTT AAATCTCAAG GAAAGGGTAC AGGTGACGAA 
GACCTTCTCA TGCTGGACAT CTATGCAATT GAAGAACTGC GTTCCCAGGG ACTTGAACCC 
ACTGATGATC TGCCAAAATA CAAATATACA TCTAATGCTG ATGGTGTTTA CGAATTTGAG 
AGTTTGATAG CAACAGTGAA AGCAATCCGC AGAGATAAGA TGTTTGTGGA AGAGGCGAGC 
ACTGGACAGG AGTGTGGGCT GGTGCTTGAC AAGACCTGTT TTTATGCAGA ACAGGGAGGG 
CAGACCTATG ATGAGGGCTA CATGGTTAAA GAGGAGGAGA GCAGTGAAGA TAAAACAGAG 
TTTACAGTGA GAAACACTCA GGTTCGGGGA GGTTACGTGC TGCACATGGG TACATTGTAC 
GGGCACTTGA AAGTTGGAGA TCAAGTGTGT CTATACGTTG ATGAGCCCAA GCGTCGTCCT 
ATTATGAGTA ACCACACAGC CACTCACATC CTGAACTTTG CCTTGCGCTC TGTTCTTGGA 
GAAGCAGATC AACGGGGTTC ATTGGTTGCC CCAGACAGGC TAAGATTTGA CTTCACAGCT 
AAAGGTGCAA TGTCCACCCA GGAGATCCGC AAAACCGAAG AGATTGCAAA TGACATTATC 
AAGGAAAATA AGGTTGTGTA TGCACTAGAT TGCTCTCTCG CAGAAGCAAA AGCTATCCAG 
GGTCTTCGTG CAGTATTTGA TGAAACATAC CCAGATCCTG TGCGTGTTGT GTCTATAGGA 
ATTCCTGTGG AAGATCTGCT GGCTGACCCA TCTAGTCCTG CTGGGTCTAT AACTTCTGTG 
GAGTTCTGTG GAGGAACGCA TCTCCAAAAT TCTGGCCATG CTGGTCCTTT CATTATTGTT 
ACAGAGGAAG CCATTGCCAA AGGAATTCGC AGAATTGTTG CTGTCACAGG TGCAGAGGCA 
CAAAAGGCCT TAAGGAGACA GGATGCCCTG AAAGTTAATC TTTCTGATCT GGAGAAGAAA 
GTGAAGAAAC AGAATTCTCC TAACAAGGAT GTACAAAAGG AAATAGCTGA CCTTAGTGAG 
AATCTCGCAA CAGCATCCAT TGCACAGTGG CAGAAGGATG TACTAAGGGA GCAGCTGAAA 
ACCTTGAAGA AAACTATGGA TGACCTGGAT CGTGCCAGCA AAGCAGATGT TCAGAAACGG 
GTATTGGAAA TAACCAAACA ACTCATAGAG GAACATCCTA ATCAGCCACT ACTTGTACTT 
GAGATGGAGA ATGGAGCATC TGCAAAGGCT TTGAACGAGT CCCTGAAACT CCTCAAGTCT 
CAGTCTCCCC ATACTGCTGC AATGATCTTC TCTGTAGATA ACGATGCAGG AAAGATCACG 
TGTTTATGCC AGGTGCCCCA GGACACTGCA GACAAAGGTC TGAAGGCCAA TGAGTGGGTG 
CAGCAAGTAT CTGATTTGAT GGATGGCAGG GGAGGAGGAA AAGACACCTC TGCCCAAGCA 
ACTGGAAAGA ATGTGGGGTG CCTGTCCCAA GTCCTAAAGC TAGCTGAAGA TTTTGCCAAA 
ATGAAACTGG AGTCCATGAA AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931707.1
CDS	134..3040
Translation	MKTCMTASEIRQKFIDFFKENAHTYVHSSATVPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLNRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKLALDLLTKEFGIDIERLYVTYFGGNEAAGLEPDLECKQIWLNLGLPESRILPGSMKDNFWEMGDTGPCGPCSEIHFDRIGGRDAAHLVNMDDPNVLEIWNLVFIQFNRETDGTLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIEKGTGARPYTGKVGAEDVDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASKGFFATLVDVVVQSLGNAFPELKKDPDMVKDIINEEETQFLKTLNRGRRILDRKINSLGEHKVIPGDTAWLLYDTYGFPVDLTSLIAEERGLTLDLESFEEERKAAQLKSQGKGTGDEDLLMLDIYAIEELRSQGLEPTDDLPKYKYTSNADGVYEFESLIATVKAIRRDKMFVEEASTGQECGLVLDKTCFYAEQGGQTYDEGYMVKEEESSEDKTEFTVRNTQVRGGYVLHMGTLYGHLKVGDQVCLYVDEPKRRPIMSNHTATHILNFALRSVLGEADQRGSLVAPDRLRFDFTAKGAMSTQEIRKTEEIANDIIKENKVVYALDCSLAEAKAIQGLRAVFDETYPDPVRVVSIGIPVEDLLADPSSPAGSITSVEFCGGTHLQNSGHAGPFIIVTEEAIAKGIRRIVAVTGAEAQKALRRQDALKVNLSDLEKKVKKQNSPNKDVQKEIADLSENLATASIAQWQKDVLREQLKTLKKTMDDLDRASKADVQKRVLEITKQLIEEHPNQPLLVLEMENGASAKALNESLKLLKSQSPHTAAMIFSVDNDAGKITCLCQVPQDTADKGLKANEWVQQVSDLMDGRGGGKDTSAQATGKNVGCLSQVLKLAEDFAKMKLESMKN

Target ORF information:

RefSeq Version	XM_002931661.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis alanyl-tRNA synthetase (aars), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931661.4

ORF Insert Sequence:

ATGAAGACGT GTATGACAGC CTCTGAAATC CGGCAGAAGT TTATTGACTT CTTTAAAGAA 
AATGCCCACA CATATGTCCA TTCTTCTGCC ACAGTCCCCC TGGATGACCC AACACTGCTC 
TTCGCCAATG CTGGAATGAA CCAGTTTAAG CCAATCTTCC TGAATACCAT TGATCCTTCT 
CATCCAATGG CAAAGCTAAA CCGAGCAGCT AATACACAGA AGTGCATCCG TGCTGGAGGC 
AAGCACAATG ATCTCGATGA TGTGGGCAAA GATGTCTATC ACCACACATT TTTTGAGATG 
CTTGGATCAT GGTCCTTTGG GGATTATTTC AAGGAGCTCG CCTGTAAACT GGCACTGGAC 
CTGCTAACCA AGGAGTTTGG CATTGACATA GAGCGCTTGT ATGTCACTTA TTTTGGCGGT 
AATGAGGCTG CTGGTCTAGA GCCTGACTTG GAATGTAAAC AAATATGGCT GAACTTGGGC 
CTGCCAGAGA GTCGTATCCT TCCAGGAAGC ATGAAGGACA ACTTTTGGGA AATGGGTGAT 
ACGGGTCCTT GTGGTCCCTG TAGTGAGATT CATTTTGACC GAATTGGGGG CCGTGATGCT 
GCCCATCTTG TGAATATGGA TGATCCAAAT GTCCTTGAGA TCTGGAACCT GGTGTTCATC 
CAGTTTAATA GGGAAACTGA TGGAACCTTA AAGCCACTGC CTAAGAAGAG CATTGATACT 
GGAATGGGAC TGGAAAGACT TGTGTCTGTG TTGCAAAATA AGATGTCCAA CTATGATACA 
GATCTGTTTG TTCCATACTT TGAAGCTATC GAAAAGGGCA CAGGTGCAAG ACCCTACACT 
GGGAAAGTGG GAGCTGAGGA TGTTGATGGC ATAGACATGG CATACAGAGT GTTGGCTGAT 
CATGCTAGAA CAATTACTGT GGCACTTGCT GATGGAGGAA GACCAGATAA CACTGGACGT 
GGATATGTTT TACGCCGCAT CCTTCGCCGT GCTGTGAGAT ACGCCCATGA GAAACTTAAT 
GCATCTAAAG GATTTTTTGC CACTCTGGTT GATGTGGTTG TCCAGTCACT GGGAAATGCT 
TTTCCAGAGC TTAAAAAAGA TCCTGATATG GTCAAAGACA TCATTAATGA AGAGGAAACA 
CAGTTCCTGA AAACACTTAA CAGGGGTCGT CGCATCTTGG ACCGAAAGAT AAACAGTCTG 
GGAGAACACA AAGTTATCCC TGGTGACACT GCCTGGCTGC TGTACGACAC CTACGGCTTC 
CCAGTGGATT TGACTAGTTT GATTGCTGAG GAACGAGGAC TGACATTGGA CTTGGAGAGC 
TTTGAAGAGG AGCGTAAAGC TGCTCAGCTT AAATCTCAAG GAAAGGGTAC AGGTGACGAA 
GACCTTCTCA TGCTGGACAT CTATGCAATT GAAGAACTGC GTTCCCAGGG ACTTGAACCC 
ACTGATGATC TGCCAAAATA CAAATATACA TCTAATGCTG ATGGTGTTTA CGAATTTGAG 
AGTTTGATAG CAACAGTGAA AGCAATCCGC AGAGATAAGA TGTTTGTGGA AGAGGCGAGC 
ACTGGACAGG AGTGTGGGCT GGTGCTTGAC AAGACCTGTT TTTATGCAGA ACAGGGAGGG 
CAGACCTATG ATGAGGGCTA CATGGTTAAA GAGGAGGAGA GCAGTGAAGA TAAAACAGAG 
TTTACAGTGA GAAACACTCA GGTTCGGGGA GGTTACGTGC TGCACATGGG TACATTGTAC 
GGGCACTTGA AAGTTGGAGA TCAAGTGTGT CTATACGTTG ATGAGCCCAA GCGTCGTCCT 
ATTATGAGTA ACCACACAGC CACTCACATC CTGAACTTTG CCTTGCGCTC TGTTCTTGGA 
GAAGCAGATC AACGGGGTTC ATTGGTTGCC CCAGACAGGC TAAGATTTGA CTTCACAGCT 
AAAGGTGCAA TGTCCACCCA GGAGATCCGC AAAACCGAAG AGATTGCAAA TGACATTATC 
AAGGAAAATA AGGTTGTGTA TGCACTAGAT TGCTCTCTCG CAGAAGCAAA AGCTATCCAG 
GGTCTTCGTG CAGTATTTGA TGAAACATAC CCAGATCCTG TGCGTGTTGT GTCTATAGGA 
ATTCCTGTGG AAGATCTGCT GGCTGACCCA TCTAGTCCTG CTGGGTCTAT AACTTCTGTG 
GAGTTCTGTG GAGGAACGCA TCTCCAAAAT TCTGGCCATG CTGGTCCTTT CATTATTGTT 
ACAGAGGAAG CCATTGCCAA AGGAATTCGC AGAATTGTTG CTGTCACAGG TGCAGAGGCA 
CAAAAGGCCT TAAGGAGACA GGATGCCCTG AAAGTTAATC TTTCTGATCT GGAGAAGAAA 
GTGAAGAAAC AGAATTCTCC TAACAAGGAT GTACAAAAGG AAATAGCTGA CCTTAGTGAG 
AATCTCGCAA CAGCATCCAT TGCACAGTGG CAGAAGGATG TACTAAGGGA GCAGCTGAAA 
ACCTTGAAGA AAACTATGGA TGACCTGGAT CGTGCCAGCA AAGCAGATGT TCAGAAACGG 
GTATTGGAAA TAACCAAACA ACTCATAGAG GAACATCCTA ATCAGCCACT ACTTGTACTT 
GAGATGGAGA ATGGAGCATC TGCAAAGGCT TTGAACGAGT CCCTGAAACT CCTCAAGTCT 
CAGTCTCCCC ATACTGCTGC AATGATCTTC TCTGTAGATA ACGATGCAGG AAAGATCACG 
TGTTTATGCC AGGTGCCCCA GGACACTGCA GACAAAGGTC TGAAGGCCAA TGAGTGGGTG 
CAGCAAGTAT CTGATTTGAT GGATGGCAGG GGAGGAGGAA AAGACACCTC TGCCCAAGCA 
ACTGGAAAGA ATGTGGGGTG CCTGTCCCAA GTCCTAAAGC TAGCTGAAGA TTTTGCCAAA 
ATGAAACTGG AGTCCATGAA AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aars ( XM_012962061.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012817515.1 Xenopus tropicalis alanyl-tRNA synthetase (aars), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa35355
Clone ID Related Accession (Same CDS sequence)	XM_012962061.2
Accession Version	XM_012962061.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alanine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962061.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAACTC TCACCATGAA GACGTGTATG ACAGCCTCTG AAATCCGGCA GAAGTTTATT 
GACTTCTTTA AAGAAAATGC CCACACATAT GTCCATTCTT CTGCCACAGT CCCCCTGGAT 
GACCCAACAC TGCTCTTCGC CAATGCTGGA ATGAACCAGT TTAAGCCAAT CTTCCTGAAT 
ACCATTGATC CTTCTCATCC AATGGCAAAG CTAAACCGAG CAGCTAATAC ACAGAAGTGC 
ATCCGTGCTG GAGGCAAGCA CAATGATCTC GATGATGTGG GCAAAGATGT CTATCACCAC 
ACATTTTTTG AGATGCTTGG ATCATGGTCC TTTGGGGATT ATTTCAAGGA GCTCGCCTGT 
AAACTGGCAC TGGACCTGCT AACCAAGGAG TTTGGCATTG ACATAGAGCG CTTGTATGTC 
ACTTATTTTG GCGGTAATGA GGCTGCTGGT CTAGAGCCTG ACTTGGAATG TAAACAAATA 
TGGCTGAACT TGGGCCTGCC AGAGAGTCGT ATCCTTCCAG GAAGCATGAA GGACAACTTT 
TGGGAAATGG GTGATACGGG TCCTTGTGGT CCCTGTAGTG AGATTCATTT TGACCGAATT 
GGGGGCCGTG ATGCTGCCCA TCTTGTGAAT ATGGATGATC CAAATGTCCT TGAGATCTGG 
AACCTGGTGT TCATCCAGTT TAATAGGGAA ACTGATGGAA CCTTAAAGCC ACTGCCTAAG 
AAGAGCATTG ATACTGGAAT GGGACTGGAA AGACTTGTGT CTGTGTTGCA AAATAAGATG 
TCCAACTATG ATACAGATCT GTTTGTTCCA TACTTTGAAG CTATCGAAAA GGGCACAGGT 
GCAAGACCCT ACACTGGGAA AGTGGGAGCT GAGGATGTTG ATGGCATAGA CATGGCATAC 
AGAGTGTTGG CTGATCATGC TAGAACAATT ACTGTGGCAC TTGCTGATGG AGGAAGACCA 
GATAACACTG GACGTGGATA TGTTTTACGC CGCATCCTTC GCCGTGCTGT GAGATACGCC 
CATGAGAAAC TTAATGCATC TAAAGGATTT TTTGCCACTC TGGTTGATGT GGTTGTCCAG 
TCACTGGGAA ATGCTTTTCC AGAGCTTAAA AAAGATCCTG ATATGGTCAA AGACATCATT 
AATGAAGAGG AAACACAGTT CCTGAAAACA CTTAACAGGG GTCGTCGCAT CTTGGACCGA 
AAGATAAACA GTCTGGGAGA ACACAAAGTT ATCCCTGGTG ACACTGCCTG GCTGCTGTAC 
GACACCTACG GCTTCCCAGT GGATTTGACT AGTTTGATTG CTGAGGAACG AGGACTGACA 
TTGGACTTGG AGAGCTTTGA AGAGGAGCGT AAAGCTGCTC AGCTTAAATC TCAAGGAAAG 
GGTACAGGTG ACGAAGACCT TCTCATGCTG GACATCTATG CAATTGAAGA ACTGCGTTCC 
CAGGGACTTG AACCCACTGA TGATCTGCCA AAATACAAAT ATACATCTAA TGCTGATGGT 
GTTTACGAAT TTGAGAGTTT GATAGCAACA GTGAAAGCAA TCCGCAGAGA TAAGATGTTT 
GTGGAAGAGG CGAGCACTGG ACAGGAGTGT GGGCTGGTGC TTGACAAGAC CTGTTTTTAT 
GCAGAACAGG GAGGGCAGAC CTATGATGAG GGCTACATGG TTAAAGAGGA GGAGAGCAGT 
GAAGATAAAA CAGAGTTTAC AGTGAGAAAC ACTCAGGTTC GGGGAGGTTA CGTGCTGCAC 
ATGGGTACAT TGTACGGGCA CTTGAAAGTT GGAGATCAAG TGTGTCTATA CGTTGATGAG 
CCCAAGCGTC GTCCTATTAT GAGTAACCAC ACAGCCACTC ACATCCTGAA CTTTGCCTTG 
CGCTCTGTTC TTGGAGAAGC AGATCAACGG GGTTCATTGG TTGCCCCAGA CAGGCTAAGA 
TTTGACTTCA CAGCTAAAGG TGCAATGTCC ACCCAGGAGA TCCGCAAAAC CGAAGAGATT 
GCAAATGACA TTATCAAGGA AAATAAGGTT GTGTATGCAC TAGATTGCTC TCTCGCAGAA 
GCAAAAGCTA TCCAGGGTCT TCGTGCAGTA TTTGATGAAA CATACCCAGA TCCTGTGCGT 
GTTGTGTCTA TAGGAATTCC TGTGGAAGAT CTGCTGGCTG ACCCATCTAG TCCTGCTGGG 
TCTATAACTT CTGTGGAGTT CTGTGGAGGA ACGCATCTCC AAAATTCTGG CCATGCTGGT 
CCTTTCATTA TTGTTACAGA GGAAGCCATT GCCAAAGGAA TTCGCAGAAT TGTTGCTGTC 
ACAGGTGCAG AGGCACAAAA GGCCTTAAGG AGACAGGATG CCCTGAAAGT TAATCTTTCT 
GATCTGGAGA AGAAAGTGAA GAAACAGAAT TCTCCTAACA AGGATGTACA AAAGGAAATA 
GCTGACCTTA GTGAGAATCT CGCAACAGCA TCCATTGCAC AGTGGCAGAA GGATGTACTA 
AGGGAGCAGC TGAAAACCTT GAAGAAAACT ATGGATGACC TGGATCGTGC CAGCAAAGCA 
GATGTTCAGA AACGGGTATT GGAAATAACC AAACAACTCA TAGAGGAACA TCCTAATCAG 
CCACTACTTG TACTTGAGAT GGAGAATGGA GCATCTGCAA AGGCTTTGAA CGAGTCCCTG 
AAACTCCTCA AGTCTCAGTC TCCCCATACT GCTGCAATGA TCTTCTCTGT AGATAACGAT 
GCAGGAAAGA TCACGTGTTT ATGCCAGGTG CCCCAGGACA CTGCAGACAA AGGTCTGAAG 
GCCAATGAGT GGGTGCAGCA AGTATCTGAT TTGATGGATG GCAGGGGAGG AGGAAAAGAC 
ACCTCTGCCC AAGCAACTGG AAAGAATGTG GGGTGCCTGT CCCAAGTCCT AAAGCTAGCT 
GAAGATTTTG CCAAAATGAA ACTGGAGTCC ATGAAAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012817515.1
CDS	13..2934
Translation	MRTLTMKTCMTASEIRQKFIDFFKENAHTYVHSSATVPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLNRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKLALDLLTKEFGIDIERLYVTYFGGNEAAGLEPDLECKQIWLNLGLPESRILPGSMKDNFWEMGDTGPCGPCSEIHFDRIGGRDAAHLVNMDDPNVLEIWNLVFIQFNRETDGTLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIEKGTGARPYTGKVGAEDVDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASKGFFATLVDVVVQSLGNAFPELKKDPDMVKDIINEEETQFLKTLNRGRRILDRKINSLGEHKVIPGDTAWLLYDTYGFPVDLTSLIAEERGLTLDLESFEEERKAAQLKSQGKGTGDEDLLMLDIYAIEELRSQGLEPTDDLPKYKYTSNADGVYEFESLIATVKAIRRDKMFVEEASTGQECGLVLDKTCFYAEQGGQTYDEGYMVKEEESSEDKTEFTVRNTQVRGGYVLHMGTLYGHLKVGDQVCLYVDEPKRRPIMSNHTATHILNFALRSVLGEADQRGSLVAPDRLRFDFTAKGAMSTQEIRKTEEIANDIIKENKVVYALDCSLAEAKAIQGLRAVFDETYPDPVRVVSIGIPVEDLLADPSSPAGSITSVEFCGGTHLQNSGHAGPFIIVTEEAIAKGIRRIVAVTGAEAQKALRRQDALKVNLSDLEKKVKKQNSPNKDVQKEIADLSENLATASIAQWQKDVLREQLKTLKKTMDDLDRASKADVQKRVLEITKQLIEEHPNQPLLVLEMENGASAKALNESLKLLKSQSPHTAAMIFSVDNDAGKITCLCQVPQDTADKGLKANEWVQQVSDLMDGRGGGKDTSAQATGKNVGCLSQVLKLAEDFAKMKLESMKN

Target ORF information:

RefSeq Version	XM_012962061.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis alanyl-tRNA synthetase (aars), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012962061.2

ORF Insert Sequence:

ATGAGAACTC TCACCATGAA GACGTGTATG ACAGCCTCTG AAATCCGGCA GAAGTTTATT 
GACTTCTTTA AAGAAAATGC CCACACATAT GTCCATTCTT CTGCCACAGT CCCCCTGGAT 
GACCCAACAC TGCTCTTCGC CAATGCTGGA ATGAACCAGT TTAAGCCAAT CTTCCTGAAT 
ACCATTGATC CTTCTCATCC AATGGCAAAG CTAAACCGAG CAGCTAATAC ACAGAAGTGC 
ATCCGTGCTG GAGGCAAGCA CAATGATCTC GATGATGTGG GCAAAGATGT CTATCACCAC 
ACATTTTTTG AGATGCTTGG ATCATGGTCC TTTGGGGATT ATTTCAAGGA GCTCGCCTGT 
AAACTGGCAC TGGACCTGCT AACCAAGGAG TTTGGCATTG ACATAGAGCG CTTGTATGTC 
ACTTATTTTG GCGGTAATGA GGCTGCTGGT CTAGAGCCTG ACTTGGAATG TAAACAAATA 
TGGCTGAACT TGGGCCTGCC AGAGAGTCGT ATCCTTCCAG GAAGCATGAA GGACAACTTT 
TGGGAAATGG GTGATACGGG TCCTTGTGGT CCCTGTAGTG AGATTCATTT TGACCGAATT 
GGGGGCCGTG ATGCTGCCCA TCTTGTGAAT ATGGATGATC CAAATGTCCT TGAGATCTGG 
AACCTGGTGT TCATCCAGTT TAATAGGGAA ACTGATGGAA CCTTAAAGCC ACTGCCTAAG 
AAGAGCATTG ATACTGGAAT GGGACTGGAA AGACTTGTGT CTGTGTTGCA AAATAAGATG 
TCCAACTATG ATACAGATCT GTTTGTTCCA TACTTTGAAG CTATCGAAAA GGGCACAGGT 
GCAAGACCCT ACACTGGGAA AGTGGGAGCT GAGGATGTTG ATGGCATAGA CATGGCATAC 
AGAGTGTTGG CTGATCATGC TAGAACAATT ACTGTGGCAC TTGCTGATGG AGGAAGACCA 
GATAACACTG GACGTGGATA TGTTTTACGC CGCATCCTTC GCCGTGCTGT GAGATACGCC 
CATGAGAAAC TTAATGCATC TAAAGGATTT TTTGCCACTC TGGTTGATGT GGTTGTCCAG 
TCACTGGGAA ATGCTTTTCC AGAGCTTAAA AAAGATCCTG ATATGGTCAA AGACATCATT 
AATGAAGAGG AAACACAGTT CCTGAAAACA CTTAACAGGG GTCGTCGCAT CTTGGACCGA 
AAGATAAACA GTCTGGGAGA ACACAAAGTT ATCCCTGGTG ACACTGCCTG GCTGCTGTAC 
GACACCTACG GCTTCCCAGT GGATTTGACT AGTTTGATTG CTGAGGAACG AGGACTGACA 
TTGGACTTGG AGAGCTTTGA AGAGGAGCGT AAAGCTGCTC AGCTTAAATC TCAAGGAAAG 
GGTACAGGTG ACGAAGACCT TCTCATGCTG GACATCTATG CAATTGAAGA ACTGCGTTCC 
CAGGGACTTG AACCCACTGA TGATCTGCCA AAATACAAAT ATACATCTAA TGCTGATGGT 
GTTTACGAAT TTGAGAGTTT GATAGCAACA GTGAAAGCAA TCCGCAGAGA TAAGATGTTT 
GTGGAAGAGG CGAGCACTGG ACAGGAGTGT GGGCTGGTGC TTGACAAGAC CTGTTTTTAT 
GCAGAACAGG GAGGGCAGAC CTATGATGAG GGCTACATGG TTAAAGAGGA GGAGAGCAGT 
GAAGATAAAA CAGAGTTTAC AGTGAGAAAC ACTCAGGTTC GGGGAGGTTA CGTGCTGCAC 
ATGGGTACAT TGTACGGGCA CTTGAAAGTT GGAGATCAAG TGTGTCTATA CGTTGATGAG 
CCCAAGCGTC GTCCTATTAT GAGTAACCAC ACAGCCACTC ACATCCTGAA CTTTGCCTTG 
CGCTCTGTTC TTGGAGAAGC AGATCAACGG GGTTCATTGG TTGCCCCAGA CAGGCTAAGA 
TTTGACTTCA CAGCTAAAGG TGCAATGTCC ACCCAGGAGA TCCGCAAAAC CGAAGAGATT 
GCAAATGACA TTATCAAGGA AAATAAGGTT GTGTATGCAC TAGATTGCTC TCTCGCAGAA 
GCAAAAGCTA TCCAGGGTCT TCGTGCAGTA TTTGATGAAA CATACCCAGA TCCTGTGCGT 
GTTGTGTCTA TAGGAATTCC TGTGGAAGAT CTGCTGGCTG ACCCATCTAG TCCTGCTGGG 
TCTATAACTT CTGTGGAGTT CTGTGGAGGA ACGCATCTCC AAAATTCTGG CCATGCTGGT 
CCTTTCATTA TTGTTACAGA GGAAGCCATT GCCAAAGGAA TTCGCAGAAT TGTTGCTGTC 
ACAGGTGCAG AGGCACAAAA GGCCTTAAGG AGACAGGATG CCCTGAAAGT TAATCTTTCT 
GATCTGGAGA AGAAAGTGAA GAAACAGAAT TCTCCTAACA AGGATGTACA AAAGGAAATA 
GCTGACCTTA GTGAGAATCT CGCAACAGCA TCCATTGCAC AGTGGCAGAA GGATGTACTA 
AGGGAGCAGC TGAAAACCTT GAAGAAAACT ATGGATGACC TGGATCGTGC CAGCAAAGCA 
GATGTTCAGA AACGGGTATT GGAAATAACC AAACAACTCA TAGAGGAACA TCCTAATCAG 
CCACTACTTG TACTTGAGAT GGAGAATGGA GCATCTGCAA AGGCTTTGAA CGAGTCCCTG 
AAACTCCTCA AGTCTCAGTC TCCCCATACT GCTGCAATGA TCTTCTCTGT AGATAACGAT 
GCAGGAAAGA TCACGTGTTT ATGCCAGGTG CCCCAGGACA CTGCAGACAA AGGTCTGAAG 
GCCAATGAGT GGGTGCAGCA AGTATCTGAT TTGATGGATG GCAGGGGAGG AGGAAAAGAC 
ACCTCTGCCC AAGCAACTGG AAAGAATGTG GGGTGCCTGT CCCAAGTCCT AAAGCTAGCT 
GAAGATTTTG CCAAAATGAA ACTGGAGTCC ATGAAAAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aars ( XM_018093363.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948852.1 Xenopus tropicalis alanyl-tRNA synthetase (aars), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa44032
Clone ID Related Accession (Same CDS sequence)	XM_018093363.1
Accession Version	XM_018093363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alanine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTTA TCCTCTGTGC CTGGAACGGG CCTTTACTAG CACAGGGGTG CCCAGACACT 
TTGTCACCCT GTGATCTACT ATTGGCTCTC ACCATGAAGA CGTGTATGAC AGCCTCTGAA 
ATCCGGCAGA AGTTTATTGA CTTCTTTAAA GAAAATGCCC ACACATATGT CCATTCTTCT 
GCCACAGTCC CCCTGGATGA CCCAACACTG CTCTTCGCCA ATGCTGGAAT GAACCAGTTT 
AAGCCAATCT TCCTGAATAC CATTGATCCT TCTCATCCAA TGGCAAAGCT AAACCGAGCA 
GCTAATACAC AGAAGTGCAT CCGTGCTGGA GGCAAGCACA ATGATCTCGA TGATGTGGGC 
AAAGATGTCT ATCACCACAC ATTTTTTGAG ATGCTTGGAT CATGGTCCTT TGGGGATTAT 
TTCAAGGAGC TCGCCTGTAA ACTGGCACTG GACCTGCTAA CCAAGGAGTT TGGCATTGAC 
ATAGAGCGCT TGTATGTCAC TTATTTTGGC GGTAATGAGG CTGCTGGTCT AGAGCCTGAC 
TTGGAATGTA AACAAATATG GCTGAACTTG GGCCTGCCAG AGAGTCGTAT CCTTCCAGGA 
AGCATGAAGG ACAACTTTTG GGAAATGGGT GATACGGGTC CTTGTGGTCC CTGTAGTGAG 
ATTCATTTTG ACCGAATTGG GGGCCGTGAT GCTGCCCATC TTGTGAATAT GGATGATCCA 
AATGTCCTTG AGATCTGGAA CCTGGTGTTC ATCCAGTTTA ATAGGGAAAC TGATGGAACC 
TTAAAGCCAC TGCCTAAGAA GAGCATTGAT ACTGGAATGG GACTGGAAAG ACTTGTGTCT 
GTGTTGCAAA ATAAGATGTC CAACTATGAT ACAGATCTGT TTGTTCCATA CTTTGAAGCT 
ATCGAAAAGG GCACAGGTGC AAGACCCTAC ACTGGGAAAG TGGGAGCTGA GGATGTTGAT 
GGCATAGACA TGGCATACAG AGTGTTGGCT GATCATGCTA GAACAATTAC TGTGGCACTT 
GCTGATGGAG GAAGACCAGA TAACACTGGA CGTGGATATG TTTTACGCCG CATCCTTCGC 
CGTGCTGTGA GATACGCCCA TGAGAAACTT AATGCATCTA AAGGATTTTT TGCCACTCTG 
GTTGATGTGG TTGTCCAGTC ACTGGGAAAT GCTTTTCCAG AGCTTAAAAA AGATCCTGAT 
ATGGTCAAAG ACATCATTAA TGAAGAGGAA ACACAGTTCC TGAAAACACT TAACAGGGGT 
CGTCGCATCT TGGACCGAAA GATAAACAGT CTGGGAGAAC ACAAAGTTAT CCCTGGTGAC 
ACTGCCTGGC TGCTGTACGA CACCTACGGC TTCCCAGTGG ATTTGACTAG TTTGATTGCT 
GAGGAACGAG GACTGACATT GGACTTGGAG AGCTTTGAAG AGGAGCGTAA AGCTGCTCAG 
CTTAAATCTC AAGGAAAGGG TACAGGTGAC GAAGACCTTC TCATGCTGGA CATCTATGCA 
ATTGAAGAAC TGCGTTCCCA GGGACTTGAA CCCACTGATG ATCTGCCAAA ATACAAATAT 
ACATCTAATG CTGATGGTGT TTACGAATTT GAGAGTTTGA TAGCAACAGT GAAAGCAATC 
CGCAGAGATA AGATGTTTGT GGAAGAGGCG AGCACTGGAC AGGAGTGTGG GCTGGTGCTT 
GACAAGACCT GTTTTTATGC AGAACAGGGA GGGCAGACCT ATGATGAGGG CTACATGGTT 
AAAGAGGAGG AGAGCAGTGA AGATAAAACA GAGTTTACAG TGAGAAACAC TCAGGTTCGG 
GGAGGTTACG TGCTGCACAT GGGTACATTG TACGGGCACT TGAAAGTTGG AGATCAAGTG 
TGTCTATACG TTGATGAGCC CAAGCGTCGT CCTATTATGA GTAACCACAC AGCCACTCAC 
ATCCTGAACT TTGCCTTGCG CTCTGTTCTT GGAGAAGCAG ATCAACGGGG TTCATTGGTT 
GCCCCAGACA GGCTAAGATT TGACTTCACA GCTAAAGGTG CAATGTCCAC CCAGGAGATC 
CGCAAAACCG AAGAGATTGC AAATGACATT ATCAAGGAAA ATAAGGTTGT GTATGCACTA 
GATTGCTCTC TCGCAGAAGC AAAAGCTATC CAGGGTCTTC GTGCAGTATT TGATGAAACA 
TACCCAGATC CTGTGCGTGT TGTGTCTATA GGAATTCCTG TGGAAGATCT GCTGGCTGAC 
CCATCTAGTC CTGCTGGGTC TATAACTTCT GTGGAGTTCT GTGGAGGAAC GCATCTCCAA 
AATTCTGGCC ATGCTGGTCC TTTCATTATT GTTACAGAGG AAGCCATTGC CAAAGGAATT 
CGCAGAATTG TTGCTGTCAC AGGTGCAGAG GCACAAAAGG CCTTAAGGAG ACAGGATGCC 
CTGAAAGTTA ATCTTTCTGA TCTGGAGAAG AAAGTGAAGA AACAGAATTC TCCTAACAAG 
GATGTACAAA AGGAAATAGC TGACCTTAGT GAGAATCTCG CAACAGCATC CATTGCACAG 
TGGCAGAAGG ATGTACTAAG GGAGCAGCTG AAAACCTTGA AGAAAACTAT GGATGACCTG 
GATCGTGCCA GCAAAGCAGA TGTTCAGAAA CGGGTATTGG AAATAACCAA ACAACTCATA 
GAGGAACATC CTAATCAGCC ACTACTTGTA CTTGAGATGG AGAATGGAGC ATCTGCAAAG 
GCTTTGAACG AGTCCCTGAA ACTCCTCAAG TCTCAGTCTC CCCATACTGC TGCAATGATC 
TTCTCTGTAG ATAACGATGC AGGAAAGATC ACGTGTTTAT GCCAGGTGCC CCAGGACACT 
GCAGACAAAG GTCTGAAGGC CAATGAGTGG GTGCAGCAAG TATCTGATTT GATGGATGGC 
AGGGGAGGAG GAAAAGACAC CTCTGCCCAA GCAACTGGAA AGAATGTGGG GTGCCTGTCC 
CAAGTCCTAA AGCTAGCTGA AGATTTTGCC AAAATGAAAC TGGAGTCCAT GAAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948852.1
CDS	16..3015
Translation	MNFILCAWNGPLLAQGCPDTLSPCDLLLALTMKTCMTASEIRQKFIDFFKENAHTYVHSSATVPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLNRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKLALDLLTKEFGIDIERLYVTYFGGNEAAGLEPDLECKQIWLNLGLPESRILPGSMKDNFWEMGDTGPCGPCSEIHFDRIGGRDAAHLVNMDDPNVLEIWNLVFIQFNRETDGTLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIEKGTGARPYTGKVGAEDVDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASKGFFATLVDVVVQSLGNAFPELKKDPDMVKDIINEEETQFLKTLNRGRRILDRKINSLGEHKVIPGDTAWLLYDTYGFPVDLTSLIAEERGLTLDLESFEEERKAAQLKSQGKGTGDEDLLMLDIYAIEELRSQGLEPTDDLPKYKYTSNADGVYEFESLIATVKAIRRDKMFVEEASTGQECGLVLDKTCFYAEQGGQTYDEGYMVKEEESSEDKTEFTVRNTQVRGGYVLHMGTLYGHLKVGDQVCLYVDEPKRRPIMSNHTATHILNFALRSVLGEADQRGSLVAPDRLRFDFTAKGAMSTQEIRKTEEIANDIIKENKVVYALDCSLAEAKAIQGLRAVFDETYPDPVRVVSIGIPVEDLLADPSSPAGSITSVEFCGGTHLQNSGHAGPFIIVTEEAIAKGIRRIVAVTGAEAQKALRRQDALKVNLSDLEKKVKKQNSPNKDVQKEIADLSENLATASIAQWQKDVLREQLKTLKKTMDDLDRASKADVQKRVLEITKQLIEEHPNQPLLVLEMENGASAKALNESLKLLKSQSPHTAAMIFSVDNDAGKITCLCQVPQDTADKGLKANEWVQQVSDLMDGRGGGKDTSAQATGKNVGCLSQVLKLAEDFAKMKLESMKN

Target ORF information:

RefSeq Version	XM_018093363.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis alanyl-tRNA synthetase (aars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093363.1

ORF Insert Sequence:

ATGAATTTTA TCCTCTGTGC CTGGAACGGG CCTTTACTAG CACAGGGGTG CCCAGACACT 
TTGTCACCCT GTGATCTACT ATTGGCTCTC ACCATGAAGA CGTGTATGAC AGCCTCTGAA 
ATCCGGCAGA AGTTTATTGA CTTCTTTAAA GAAAATGCCC ACACATATGT CCATTCTTCT 
GCCACAGTCC CCCTGGATGA CCCAACACTG CTCTTCGCCA ATGCTGGAAT GAACCAGTTT 
AAGCCAATCT TCCTGAATAC CATTGATCCT TCTCATCCAA TGGCAAAGCT AAACCGAGCA 
GCTAATACAC AGAAGTGCAT CCGTGCTGGA GGCAAGCACA ATGATCTCGA TGATGTGGGC 
AAAGATGTCT ATCACCACAC ATTTTTTGAG ATGCTTGGAT CATGGTCCTT TGGGGATTAT 
TTCAAGGAGC TCGCCTGTAA ACTGGCACTG GACCTGCTAA CCAAGGAGTT TGGCATTGAC 
ATAGAGCGCT TGTATGTCAC TTATTTTGGC GGTAATGAGG CTGCTGGTCT AGAGCCTGAC 
TTGGAATGTA AACAAATATG GCTGAACTTG GGCCTGCCAG AGAGTCGTAT CCTTCCAGGA 
AGCATGAAGG ACAACTTTTG GGAAATGGGT GATACGGGTC CTTGTGGTCC CTGTAGTGAG 
ATTCATTTTG ACCGAATTGG GGGCCGTGAT GCTGCCCATC TTGTGAATAT GGATGATCCA 
AATGTCCTTG AGATCTGGAA CCTGGTGTTC ATCCAGTTTA ATAGGGAAAC TGATGGAACC 
TTAAAGCCAC TGCCTAAGAA GAGCATTGAT ACTGGAATGG GACTGGAAAG ACTTGTGTCT 
GTGTTGCAAA ATAAGATGTC CAACTATGAT ACAGATCTGT TTGTTCCATA CTTTGAAGCT 
ATCGAAAAGG GCACAGGTGC AAGACCCTAC ACTGGGAAAG TGGGAGCTGA GGATGTTGAT 
GGCATAGACA TGGCATACAG AGTGTTGGCT GATCATGCTA GAACAATTAC TGTGGCACTT 
GCTGATGGAG GAAGACCAGA TAACACTGGA CGTGGATATG TTTTACGCCG CATCCTTCGC 
CGTGCTGTGA GATACGCCCA TGAGAAACTT AATGCATCTA AAGGATTTTT TGCCACTCTG 
GTTGATGTGG TTGTCCAGTC ACTGGGAAAT GCTTTTCCAG AGCTTAAAAA AGATCCTGAT 
ATGGTCAAAG ACATCATTAA TGAAGAGGAA ACACAGTTCC TGAAAACACT TAACAGGGGT 
CGTCGCATCT TGGACCGAAA GATAAACAGT CTGGGAGAAC ACAAAGTTAT CCCTGGTGAC 
ACTGCCTGGC TGCTGTACGA CACCTACGGC TTCCCAGTGG ATTTGACTAG TTTGATTGCT 
GAGGAACGAG GACTGACATT GGACTTGGAG AGCTTTGAAG AGGAGCGTAA AGCTGCTCAG 
CTTAAATCTC AAGGAAAGGG TACAGGTGAC GAAGACCTTC TCATGCTGGA CATCTATGCA 
ATTGAAGAAC TGCGTTCCCA GGGACTTGAA CCCACTGATG ATCTGCCAAA ATACAAATAT 
ACATCTAATG CTGATGGTGT TTACGAATTT GAGAGTTTGA TAGCAACAGT GAAAGCAATC 
CGCAGAGATA AGATGTTTGT GGAAGAGGCG AGCACTGGAC AGGAGTGTGG GCTGGTGCTT 
GACAAGACCT GTTTTTATGC AGAACAGGGA GGGCAGACCT ATGATGAGGG CTACATGGTT 
AAAGAGGAGG AGAGCAGTGA AGATAAAACA GAGTTTACAG TGAGAAACAC TCAGGTTCGG 
GGAGGTTACG TGCTGCACAT GGGTACATTG TACGGGCACT TGAAAGTTGG AGATCAAGTG 
TGTCTATACG TTGATGAGCC CAAGCGTCGT CCTATTATGA GTAACCACAC AGCCACTCAC 
ATCCTGAACT TTGCCTTGCG CTCTGTTCTT GGAGAAGCAG ATCAACGGGG TTCATTGGTT 
GCCCCAGACA GGCTAAGATT TGACTTCACA GCTAAAGGTG CAATGTCCAC CCAGGAGATC 
CGCAAAACCG AAGAGATTGC AAATGACATT ATCAAGGAAA ATAAGGTTGT GTATGCACTA 
GATTGCTCTC TCGCAGAAGC AAAAGCTATC CAGGGTCTTC GTGCAGTATT TGATGAAACA 
TACCCAGATC CTGTGCGTGT TGTGTCTATA GGAATTCCTG TGGAAGATCT GCTGGCTGAC 
CCATCTAGTC CTGCTGGGTC TATAACTTCT GTGGAGTTCT GTGGAGGAAC GCATCTCCAA 
AATTCTGGCC ATGCTGGTCC TTTCATTATT GTTACAGAGG AAGCCATTGC CAAAGGAATT 
CGCAGAATTG TTGCTGTCAC AGGTGCAGAG GCACAAAAGG CCTTAAGGAG ACAGGATGCC 
CTGAAAGTTA ATCTTTCTGA TCTGGAGAAG AAAGTGAAGA AACAGAATTC TCCTAACAAG 
GATGTACAAA AGGAAATAGC TGACCTTAGT GAGAATCTCG CAACAGCATC CATTGCACAG 
TGGCAGAAGG ATGTACTAAG GGAGCAGCTG AAAACCTTGA AGAAAACTAT GGATGACCTG 
GATCGTGCCA GCAAAGCAGA TGTTCAGAAA CGGGTATTGG AAATAACCAA ACAACTCATA 
GAGGAACATC CTAATCAGCC ACTACTTGTA CTTGAGATGG AGAATGGAGC ATCTGCAAAG 
GCTTTGAACG AGTCCCTGAA ACTCCTCAAG TCTCAGTCTC CCCATACTGC TGCAATGATC 
TTCTCTGTAG ATAACGATGC AGGAAAGATC ACGTGTTTAT GCCAGGTGCC CCAGGACACT 
GCAGACAAAG GTCTGAAGGC CAATGAGTGG GTGCAGCAAG TATCTGATTT GATGGATGGC 
AGGGGAGGAG GAAAAGACAC CTCTGCCCAA GCAACTGGAA AGAATGTGGG GTGCCTGTCC 
CAAGTCCTAA AGCTAGCTGA AGATTTTGCC AAAATGAAAC TGGAGTCCAT GAAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aars ( XM_018093363.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017948852.2 Xenopus tropicalis alanyl-tRNA synthetase 1 (aars1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa56966
Clone ID Related Accession (Same CDS sequence)	XM_018093363.2
Accession Version	XM_018093363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alanine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTTTA TCCTCTGTGC CTGGAACGGG CCTTTACTAG CACAGGGGTG CCCAGACACT 
TTGTCACCCT GTGATCTACT ATTGGCTCTC ACCATGAAGA CGTGTATGAC AGCCTCTGAA 
ATCCGGCAGA AGTTTATTGA CTTCTTTAAA GAAAATGCCC ACACATATGT CCATTCTTCT 
GCCACAGTCC CCCTGGATGA CCCAACACTG CTCTTCGCCA ATGCTGGAAT GAACCAGTTT 
AAGCCAATCT TCCTGAATAC CATTGATCCT TCTCATCCAA TGGCAAAGCT AAACCGAGCA 
GCTAATACAC AGAAGTGCAT CCGTGCTGGA GGCAAGCACA ATGATCTCGA TGATGTGGGC 
AAAGATGTCT ATCACCACAC ATTTTTTGAG ATGCTTGGAT CATGGTCCTT TGGGGATTAT 
TTCAAGGAGC TCGCCTGTAA ACTGGCACTG GACCTGCTAA CCAAGGAGTT TGGCATTGAC 
ATAGAGCGCT TGTATGTCAC TTATTTTGGC GGTAATGAGG CTGCTGGTCT AGAGCCTGAC 
TTGGAATGTA AACAAATATG GCTGAACTTG GGCCTGCCAG AGAGTCGTAT CCTTCCAGGA 
AGCATGAAGG ACAACTTTTG GGAAATGGGT GATACGGGTC CTTGTGGTCC CTGTAGTGAG 
ATTCATTTTG ACCGAATTGG GGGCCGTGAT GCTGCCCATC TTGTGAATAT GGATGATCCA 
AATGTCCTTG AGATCTGGAA CCTGGTGTTC ATCCAGTTTA ATAGGGAAAC TGATGGAACC 
TTAAAGCCAC TGCCTAAGAA GAGCATTGAT ACTGGAATGG GACTGGAAAG ACTTGTGTCT 
GTGTTGCAAA ATAAGATGTC CAACTATGAT ACAGATCTGT TTGTTCCATA CTTTGAAGCT 
ATCGAAAAGG GCACAGGTGC AAGACCCTAC ACTGGGAAAG TGGGAGCTGA GGATGTTGAT 
GGCATAGACA TGGCATACAG AGTGTTGGCT GATCATGCTA GAACAATTAC TGTGGCACTT 
GCTGATGGAG GAAGACCAGA TAACACTGGA CGTGGATATG TTTTACGCCG CATCCTTCGC 
CGTGCTGTGA GATACGCCCA TGAGAAACTT AATGCATCTA AAGGATTTTT TGCCACTCTG 
GTTGATGTGG TTGTCCAGTC ACTGGGAAAT GCTTTTCCAG AGCTTAAAAA AGATCCTGAT 
ATGGTCAAAG ACATCATTAA TGAAGAGGAA ACACAGTTCC TGAAAACACT TAACAGGGGT 
CGTCGCATCT TGGACCGAAA GATAAACAGT CTGGGAGAAC ACAAAGTTAT CCCTGGTGAC 
ACTGCCTGGC TGCTGTACGA CACCTACGGC TTCCCAGTGG ATTTGACTAG TTTGATTGCT 
GAGGAACGAG GACTGACATT GGACTTGGAG AGCTTTGAAG AGGAGCGTAA AGCTGCTCAG 
CTTAAATCTC AAGGAAAGGG TACAGGTGAC GAAGACCTTC TCATGCTGGA CATCTATGCA 
ATTGAAGAAC TGCGTTCCCA GGGACTTGAA CCCACTGATG ATCTGCCAAA ATACAAATAT 
ACATCTAATG CTGATGGTGT TTACGAATTT GAGAGTTTGA TAGCAACAGT GAAAGCAATC 
CGCAGAGATA AGATGTTTGT GGAAGAGGCG AGCACTGGAC AGGAGTGTGG GCTGGTGCTT 
GACAAGACCT GTTTTTATGC AGAACAGGGA GGGCAGACCT ATGATGAGGG CTACATGGTT 
AAAGAGGAGG AGAGCAGTGA AGATAAAACA GAGTTTACAG TGAGAAACAC TCAGGTTCGG 
GGAGGTTACG TGCTGCACAT GGGTACATTG TACGGGCACT TGAAAGTTGG AGATCAAGTG 
CGTCTATACG TTGATGAGCC CAAGCGTCGT CCTATTATGA GTAACCACAC AGCCACTCAC 
ATCCTGAACT TTGCCTTGCG CTCTGTTCTT GGAGAAGCAG ATCAACGGGG TTCATTGGTT 
GCCCCAGACA GGCTAAGATT TGACTTCACA GCTAAAGGTG CAATGTCCAC CCAGGAGATC 
CGCAAAACCG AAGAGATTGC AAATGACATT ATCAAGGAAA ATAAGGTTGT GTATGCACTA 
GATTGCTCTC TCGCAGAAGC AAAAGCTATC CAGGGTCTTC GTGCAGTATT TGATGAAACA 
TACCCAGATC CTGTGCGTGT TGTGTCTATA GGAATTCCTG TGGAAGATCT GCTGGCTGAC 
CCATCTAGTC CTGCTGGGTC TATAACTTCT GTGGAGTTCT GTGGAGGAAC GCATCTCCAA 
AATTCTGGCC ATGCTGGTCC TTTCATTATT GTTACAGAGG AAGCCATTGC CAAAGGAATT 
CGCAGAATTG TTGCTGTCAC AGGTGCAGAG GCACAAAAGG CCTTAAGGAG ACAGGATGCC 
CTGAAAGTTA ATCTTTCTGA TCTGGAGAAG AAAGTGAAGA AACAGAATTC TCCTAACAAG 
GATGTACAAA AGGAAATAGC TGACCTTAGT GAGAATCTCG CAACAGCATC CATTGCACAG 
TGGCAGAAGG ATGTACTAAG GGAGCAGCTG AAAACCTTGA AGAAAACTAT GGATGACCTG 
GATCGTGCCA GCAAAGCAGA TGTTCAGAAA CGGGTATTGG AAATAACCAA ACAACTCATA 
GAGGAACATC CTAATCAGCC ACTACTTGTA CTTGAGATGG AGAATGGAGC ATCTGCAAAG 
GCTTTGAACG AGTCCCTGAA ACTTCTCAAG TCTCAGTCTC CCCATACTGC TGCAATGATC 
TTCTCTGTAG ATAACGATGC AGGAAAGATC ACGTGTTTAT GCCAGGTGCC CCAGGACACT 
GCAGACAAAG GTCTGAAGGC CAATGAGTGG GTGCAGCAAG TATCTGATTT GATGGATGGC 
AGGGGAGGAG GAAAAGACAC CTCTGCCCAA GCAACTGGAA AGAATGTGGG GTGCCTGTCC 
CAAGTCCTAA AGCTAGCTGA AGATTTTGCC AAAATGAAAC TGGAGTCCAT GAAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017948852.2
CDS	18..3017
Translation	MNFILCAWNGPLLAQGCPDTLSPCDLLLALTMKTCMTASEIRQKFIDFFKENAHTYVHSSATVPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLNRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKLALDLLTKEFGIDIERLYVTYFGGNEAAGLEPDLECKQIWLNLGLPESRILPGSMKDNFWEMGDTGPCGPCSEIHFDRIGGRDAAHLVNMDDPNVLEIWNLVFIQFNRETDGTLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIEKGTGARPYTGKVGAEDVDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASKGFFATLVDVVVQSLGNAFPELKKDPDMVKDIINEEETQFLKTLNRGRRILDRKINSLGEHKVIPGDTAWLLYDTYGFPVDLTSLIAEERGLTLDLESFEEERKAAQLKSQGKGTGDEDLLMLDIYAIEELRSQGLEPTDDLPKYKYTSNADGVYEFESLIATVKAIRRDKMFVEEASTGQECGLVLDKTCFYAEQGGQTYDEGYMVKEEESSEDKTEFTVRNTQVRGGYVLHMGTLYGHLKVGDQVRLYVDEPKRRPIMSNHTATHILNFALRSVLGEADQRGSLVAPDRLRFDFTAKGAMSTQEIRKTEEIANDIIKENKVVYALDCSLAEAKAIQGLRAVFDETYPDPVRVVSIGIPVEDLLADPSSPAGSITSVEFCGGTHLQNSGHAGPFIIVTEEAIAKGIRRIVAVTGAEAQKALRRQDALKVNLSDLEKKVKKQNSPNKDVQKEIADLSENLATASIAQWQKDVLREQLKTLKKTMDDLDRASKADVQKRVLEITKQLIEEHPNQPLLVLEMENGASAKALNESLKLLKSQSPHTAAMIFSVDNDAGKITCLCQVPQDTADKGLKANEWVQQVSDLMDGRGGGKDTSAQATGKNVGCLSQVLKLAEDFAKMKLESMKN

Target ORF information:

RefSeq Version	XM_018093363.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis alanyl-tRNA synthetase 1 (aars1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093363.2

ORF Insert Sequence:

ATGAATTTTA TCCTCTGTGC CTGGAACGGG CCTTTACTAG CACAGGGGTG CCCAGACACT 
TTGTCACCCT GTGATCTACT ATTGGCTCTC ACCATGAAGA CGTGTATGAC AGCCTCTGAA 
ATCCGGCAGA AGTTTATTGA CTTCTTTAAA GAAAATGCCC ACACATATGT CCATTCTTCT 
GCCACAGTCC CCCTGGATGA CCCAACACTG CTCTTCGCCA ATGCTGGAAT GAACCAGTTT 
AAGCCAATCT TCCTGAATAC CATTGATCCT TCTCATCCAA TGGCAAAGCT AAACCGAGCA 
GCTAATACAC AGAAGTGCAT CCGTGCTGGA GGCAAGCACA ATGATCTCGA TGATGTGGGC 
AAAGATGTCT ATCACCACAC ATTTTTTGAG ATGCTTGGAT CATGGTCCTT TGGGGATTAT 
TTCAAGGAGC TCGCCTGTAA ACTGGCACTG GACCTGCTAA CCAAGGAGTT TGGCATTGAC 
ATAGAGCGCT TGTATGTCAC TTATTTTGGC GGTAATGAGG CTGCTGGTCT AGAGCCTGAC 
TTGGAATGTA AACAAATATG GCTGAACTTG GGCCTGCCAG AGAGTCGTAT CCTTCCAGGA 
AGCATGAAGG ACAACTTTTG GGAAATGGGT GATACGGGTC CTTGTGGTCC CTGTAGTGAG 
ATTCATTTTG ACCGAATTGG GGGCCGTGAT GCTGCCCATC TTGTGAATAT GGATGATCCA 
AATGTCCTTG AGATCTGGAA CCTGGTGTTC ATCCAGTTTA ATAGGGAAAC TGATGGAACC 
TTAAAGCCAC TGCCTAAGAA GAGCATTGAT ACTGGAATGG GACTGGAAAG ACTTGTGTCT 
GTGTTGCAAA ATAAGATGTC CAACTATGAT ACAGATCTGT TTGTTCCATA CTTTGAAGCT 
ATCGAAAAGG GCACAGGTGC AAGACCCTAC ACTGGGAAAG TGGGAGCTGA GGATGTTGAT 
GGCATAGACA TGGCATACAG AGTGTTGGCT GATCATGCTA GAACAATTAC TGTGGCACTT 
GCTGATGGAG GAAGACCAGA TAACACTGGA CGTGGATATG TTTTACGCCG CATCCTTCGC 
CGTGCTGTGA GATACGCCCA TGAGAAACTT AATGCATCTA AAGGATTTTT TGCCACTCTG 
GTTGATGTGG TTGTCCAGTC ACTGGGAAAT GCTTTTCCAG AGCTTAAAAA AGATCCTGAT 
ATGGTCAAAG ACATCATTAA TGAAGAGGAA ACACAGTTCC TGAAAACACT TAACAGGGGT 
CGTCGCATCT TGGACCGAAA GATAAACAGT CTGGGAGAAC ACAAAGTTAT CCCTGGTGAC 
ACTGCCTGGC TGCTGTACGA CACCTACGGC TTCCCAGTGG ATTTGACTAG TTTGATTGCT 
GAGGAACGAG GACTGACATT GGACTTGGAG AGCTTTGAAG AGGAGCGTAA AGCTGCTCAG 
CTTAAATCTC AAGGAAAGGG TACAGGTGAC GAAGACCTTC TCATGCTGGA CATCTATGCA 
ATTGAAGAAC TGCGTTCCCA GGGACTTGAA CCCACTGATG ATCTGCCAAA ATACAAATAT 
ACATCTAATG CTGATGGTGT TTACGAATTT GAGAGTTTGA TAGCAACAGT GAAAGCAATC 
CGCAGAGATA AGATGTTTGT GGAAGAGGCG AGCACTGGAC AGGAGTGTGG GCTGGTGCTT 
GACAAGACCT GTTTTTATGC AGAACAGGGA GGGCAGACCT ATGATGAGGG CTACATGGTT 
AAAGAGGAGG AGAGCAGTGA AGATAAAACA GAGTTTACAG TGAGAAACAC TCAGGTTCGG 
GGAGGTTACG TGCTGCACAT GGGTACATTG TACGGGCACT TGAAAGTTGG AGATCAAGTG 
CGTCTATACG TTGATGAGCC CAAGCGTCGT CCTATTATGA GTAACCACAC AGCCACTCAC 
ATCCTGAACT TTGCCTTGCG CTCTGTTCTT GGAGAAGCAG ATCAACGGGG TTCATTGGTT 
GCCCCAGACA GGCTAAGATT TGACTTCACA GCTAAAGGTG CAATGTCCAC CCAGGAGATC 
CGCAAAACCG AAGAGATTGC AAATGACATT ATCAAGGAAA ATAAGGTTGT GTATGCACTA 
GATTGCTCTC TCGCAGAAGC AAAAGCTATC CAGGGTCTTC GTGCAGTATT TGATGAAACA 
TACCCAGATC CTGTGCGTGT TGTGTCTATA GGAATTCCTG TGGAAGATCT GCTGGCTGAC 
CCATCTAGTC CTGCTGGGTC TATAACTTCT GTGGAGTTCT GTGGAGGAAC GCATCTCCAA 
AATTCTGGCC ATGCTGGTCC TTTCATTATT GTTACAGAGG AAGCCATTGC CAAAGGAATT 
CGCAGAATTG TTGCTGTCAC AGGTGCAGAG GCACAAAAGG CCTTAAGGAG ACAGGATGCC 
CTGAAAGTTA ATCTTTCTGA TCTGGAGAAG AAAGTGAAGA AACAGAATTC TCCTAACAAG 
GATGTACAAA AGGAAATAGC TGACCTTAGT GAGAATCTCG CAACAGCATC CATTGCACAG 
TGGCAGAAGG ATGTACTAAG GGAGCAGCTG AAAACCTTGA AGAAAACTAT GGATGACCTG 
GATCGTGCCA GCAAAGCAGA TGTTCAGAAA CGGGTATTGG AAATAACCAA ACAACTCATA 
GAGGAACATC CTAATCAGCC ACTACTTGTA CTTGAGATGG AGAATGGAGC ATCTGCAAAG 
GCTTTGAACG AGTCCCTGAA ACTTCTCAAG TCTCAGTCTC CCCATACTGC TGCAATGATC 
TTCTCTGTAG ATAACGATGC AGGAAAGATC ACGTGTTTAT GCCAGGTGCC CCAGGACACT 
GCAGACAAAG GTCTGAAGGC CAATGAGTGG GTGCAGCAAG TATCTGATTT GATGGATGGC 
AGGGGAGGAG GAAAAGACAC CTCTGCCCAA GCAACTGGAA AGAATGTGGG GTGCCTGTCC 
CAAGTCCTAA AGCTAGCTGA AGATTTTGCC AAAATGAAAC TGGAGTCCAT GAAAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aars ( XM_004914051.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914108.2 Xenopus tropicalis alanyl-tRNA synthetase 1 (aars1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa56967
Clone ID Related Accession (Same CDS sequence)	XM_004914051.4 , XM_002931661.5
Accession Version	XM_004914051.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alanine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914051.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGT GTATGACAGC CTCTGAAATC CGGCAGAAGT TTATTGACTT CTTTAAAGAA 
AATGCCCACA CATATGTCCA TTCTTCTGCC ACAGTCCCCC TGGATGACCC AACACTGCTC 
TTCGCCAATG CTGGAATGAA CCAGTTTAAG CCAATCTTCC TGAATACCAT TGATCCTTCT 
CATCCAATGG CAAAGCTAAA CCGAGCAGCT AATACACAGA AGTGCATCCG TGCTGGAGGC 
AAGCACAATG ATCTCGATGA TGTGGGCAAA GATGTCTATC ACCACACATT TTTTGAGATG 
CTTGGATCAT GGTCCTTTGG GGATTATTTC AAGGAGCTCG CCTGTAAACT GGCACTGGAC 
CTGCTAACCA AGGAGTTTGG CATTGACATA GAGCGCTTGT ATGTCACTTA TTTTGGCGGT 
AATGAGGCTG CTGGTCTAGA GCCTGACTTG GAATGTAAAC AAATATGGCT GAACTTGGGC 
CTGCCAGAGA GTCGTATCCT TCCAGGAAGC ATGAAGGACA ACTTTTGGGA AATGGGTGAT 
ACGGGTCCTT GTGGTCCCTG TAGTGAGATT CATTTTGACC GAATTGGGGG CCGTGATGCT 
GCCCATCTTG TGAATATGGA TGATCCAAAT GTCCTTGAGA TCTGGAACCT GGTGTTCATC 
CAGTTTAATA GGGAAACTGA TGGAACCTTA AAGCCACTGC CTAAGAAGAG CATTGATACT 
GGAATGGGAC TGGAAAGACT TGTGTCTGTG TTGCAAAATA AGATGTCCAA CTATGATACA 
GATCTGTTTG TTCCATACTT TGAAGCTATC GAAAAGGGCA CAGGTGCAAG ACCCTACACT 
GGGAAAGTGG GAGCTGAGGA TGTTGATGGC ATAGACATGG CATACAGAGT GTTGGCTGAT 
CATGCTAGAA CAATTACTGT GGCACTTGCT GATGGAGGAA GACCAGATAA CACTGGACGT 
GGATATGTTT TACGCCGCAT CCTTCGCCGT GCTGTGAGAT ACGCCCATGA GAAACTTAAT 
GCATCTAAAG GATTTTTTGC CACTCTGGTT GATGTGGTTG TCCAGTCACT GGGAAATGCT 
TTTCCAGAGC TTAAAAAAGA TCCTGATATG GTCAAAGACA TCATTAATGA AGAGGAAACA 
CAGTTCCTGA AAACACTTAA CAGGGGTCGT CGCATCTTGG ACCGAAAGAT AAACAGTCTG 
GGAGAACACA AAGTTATCCC TGGTGACACT GCCTGGCTGC TGTACGACAC CTACGGCTTC 
CCAGTGGATT TGACTAGTTT GATTGCTGAG GAACGAGGAC TGACATTGGA CTTGGAGAGC 
TTTGAAGAGG AGCGTAAAGC TGCTCAGCTT AAATCTCAAG GAAAGGGTAC AGGTGACGAA 
GACCTTCTCA TGCTGGACAT CTATGCAATT GAAGAACTGC GTTCCCAGGG ACTTGAACCC 
ACTGATGATC TGCCAAAATA CAAATATACA TCTAATGCTG ATGGTGTTTA CGAATTTGAG 
AGTTTGATAG CAACAGTGAA AGCAATCCGC AGAGATAAGA TGTTTGTGGA AGAGGCGAGC 
ACTGGACAGG AGTGTGGGCT GGTGCTTGAC AAGACCTGTT TTTATGCAGA ACAGGGAGGG 
CAGACCTATG ATGAGGGCTA CATGGTTAAA GAGGAGGAGA GCAGTGAAGA TAAAACAGAG 
TTTACAGTGA GAAACACTCA GGTTCGGGGA GGTTACGTGC TGCACATGGG TACATTGTAC 
GGGCACTTGA AAGTTGGAGA TCAAGTGCGT CTATACGTTG ATGAGCCCAA GCGTCGTCCT 
ATTATGAGTA ACCACACAGC CACTCACATC CTGAACTTTG CCTTGCGCTC TGTTCTTGGA 
GAAGCAGATC AACGGGGTTC ATTGGTTGCC CCAGACAGGC TAAGATTTGA CTTCACAGCT 
AAAGGTGCAA TGTCCACCCA GGAGATCCGC AAAACCGAAG AGATTGCAAA TGACATTATC 
AAGGAAAATA AGGTTGTGTA TGCACTAGAT TGCTCTCTCG CAGAAGCAAA AGCTATCCAG 
GGTCTTCGTG CAGTATTTGA TGAAACATAC CCAGATCCTG TGCGTGTTGT GTCTATAGGA 
ATTCCTGTGG AAGATCTGCT GGCTGACCCA TCTAGTCCTG CTGGGTCTAT AACTTCTGTG 
GAGTTCTGTG GAGGAACGCA TCTCCAAAAT TCTGGCCATG CTGGTCCTTT CATTATTGTT 
ACAGAGGAAG CCATTGCCAA AGGAATTCGC AGAATTGTTG CTGTCACAGG TGCAGAGGCA 
CAAAAGGCCT TAAGGAGACA GGATGCCCTG AAAGTTAATC TTTCTGATCT GGAGAAGAAA 
GTGAAGAAAC AGAATTCTCC TAACAAGGAT GTACAAAAGG AAATAGCTGA CCTTAGTGAG 
AATCTCGCAA CAGCATCCAT TGCACAGTGG CAGAAGGATG TACTAAGGGA GCAGCTGAAA 
ACCTTGAAGA AAACTATGGA TGACCTGGAT CGTGCCAGCA AAGCAGATGT TCAGAAACGG 
GTATTGGAAA TAACCAAACA ACTCATAGAG GAACATCCTA ATCAGCCACT ACTTGTACTT 
GAGATGGAGA ATGGAGCATC TGCAAAGGCT TTGAACGAGT CCCTGAAACT TCTCAAGTCT 
CAGTCTCCCC ATACTGCTGC AATGATCTTC TCTGTAGATA ACGATGCAGG AAAGATCACG 
TGTTTATGCC AGGTGCCCCA GGACACTGCA GACAAAGGTC TGAAGGCCAA TGAGTGGGTG 
CAGCAAGTAT CTGATTTGAT GGATGGCAGG GGAGGAGGAA AAGACACCTC TGCCCAAGCA 
ACTGGAAAGA ATGTGGGGTG CCTGTCCCAA GTCCTAAAGC TAGCTGAAGA TTTTGCCAAA 
ATGAAACTGG AGTCCATGAA AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914108.2
CDS	113..3019
Translation	MKTCMTASEIRQKFIDFFKENAHTYVHSSATVPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLNRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKLALDLLTKEFGIDIERLYVTYFGGNEAAGLEPDLECKQIWLNLGLPESRILPGSMKDNFWEMGDTGPCGPCSEIHFDRIGGRDAAHLVNMDDPNVLEIWNLVFIQFNRETDGTLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIEKGTGARPYTGKVGAEDVDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASKGFFATLVDVVVQSLGNAFPELKKDPDMVKDIINEEETQFLKTLNRGRRILDRKINSLGEHKVIPGDTAWLLYDTYGFPVDLTSLIAEERGLTLDLESFEEERKAAQLKSQGKGTGDEDLLMLDIYAIEELRSQGLEPTDDLPKYKYTSNADGVYEFESLIATVKAIRRDKMFVEEASTGQECGLVLDKTCFYAEQGGQTYDEGYMVKEEESSEDKTEFTVRNTQVRGGYVLHMGTLYGHLKVGDQVRLYVDEPKRRPIMSNHTATHILNFALRSVLGEADQRGSLVAPDRLRFDFTAKGAMSTQEIRKTEEIANDIIKENKVVYALDCSLAEAKAIQGLRAVFDETYPDPVRVVSIGIPVEDLLADPSSPAGSITSVEFCGGTHLQNSGHAGPFIIVTEEAIAKGIRRIVAVTGAEAQKALRRQDALKVNLSDLEKKVKKQNSPNKDVQKEIADLSENLATASIAQWQKDVLREQLKTLKKTMDDLDRASKADVQKRVLEITKQLIEEHPNQPLLVLEMENGASAKALNESLKLLKSQSPHTAAMIFSVDNDAGKITCLCQVPQDTADKGLKANEWVQQVSDLMDGRGGGKDTSAQATGKNVGCLSQVLKLAEDFAKMKLESMKN

Target ORF information:

RefSeq Version	XM_004914051.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis alanyl-tRNA synthetase 1 (aars1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914051.4

ORF Insert Sequence:

ATGAAGACGT GTATGACAGC CTCTGAAATC CGGCAGAAGT TTATTGACTT CTTTAAAGAA 
AATGCCCACA CATATGTCCA TTCTTCTGCC ACAGTCCCCC TGGATGACCC AACACTGCTC 
TTCGCCAATG CTGGAATGAA CCAGTTTAAG CCAATCTTCC TGAATACCAT TGATCCTTCT 
CATCCAATGG CAAAGCTAAA CCGAGCAGCT AATACACAGA AGTGCATCCG TGCTGGAGGC 
AAGCACAATG ATCTCGATGA TGTGGGCAAA GATGTCTATC ACCACACATT TTTTGAGATG 
CTTGGATCAT GGTCCTTTGG GGATTATTTC AAGGAGCTCG CCTGTAAACT GGCACTGGAC 
CTGCTAACCA AGGAGTTTGG CATTGACATA GAGCGCTTGT ATGTCACTTA TTTTGGCGGT 
AATGAGGCTG CTGGTCTAGA GCCTGACTTG GAATGTAAAC AAATATGGCT GAACTTGGGC 
CTGCCAGAGA GTCGTATCCT TCCAGGAAGC ATGAAGGACA ACTTTTGGGA AATGGGTGAT 
ACGGGTCCTT GTGGTCCCTG TAGTGAGATT CATTTTGACC GAATTGGGGG CCGTGATGCT 
GCCCATCTTG TGAATATGGA TGATCCAAAT GTCCTTGAGA TCTGGAACCT GGTGTTCATC 
CAGTTTAATA GGGAAACTGA TGGAACCTTA AAGCCACTGC CTAAGAAGAG CATTGATACT 
GGAATGGGAC TGGAAAGACT TGTGTCTGTG TTGCAAAATA AGATGTCCAA CTATGATACA 
GATCTGTTTG TTCCATACTT TGAAGCTATC GAAAAGGGCA CAGGTGCAAG ACCCTACACT 
GGGAAAGTGG GAGCTGAGGA TGTTGATGGC ATAGACATGG CATACAGAGT GTTGGCTGAT 
CATGCTAGAA CAATTACTGT GGCACTTGCT GATGGAGGAA GACCAGATAA CACTGGACGT 
GGATATGTTT TACGCCGCAT CCTTCGCCGT GCTGTGAGAT ACGCCCATGA GAAACTTAAT 
GCATCTAAAG GATTTTTTGC CACTCTGGTT GATGTGGTTG TCCAGTCACT GGGAAATGCT 
TTTCCAGAGC TTAAAAAAGA TCCTGATATG GTCAAAGACA TCATTAATGA AGAGGAAACA 
CAGTTCCTGA AAACACTTAA CAGGGGTCGT CGCATCTTGG ACCGAAAGAT AAACAGTCTG 
GGAGAACACA AAGTTATCCC TGGTGACACT GCCTGGCTGC TGTACGACAC CTACGGCTTC 
CCAGTGGATT TGACTAGTTT GATTGCTGAG GAACGAGGAC TGACATTGGA CTTGGAGAGC 
TTTGAAGAGG AGCGTAAAGC TGCTCAGCTT AAATCTCAAG GAAAGGGTAC AGGTGACGAA 
GACCTTCTCA TGCTGGACAT CTATGCAATT GAAGAACTGC GTTCCCAGGG ACTTGAACCC 
ACTGATGATC TGCCAAAATA CAAATATACA TCTAATGCTG ATGGTGTTTA CGAATTTGAG 
AGTTTGATAG CAACAGTGAA AGCAATCCGC AGAGATAAGA TGTTTGTGGA AGAGGCGAGC 
ACTGGACAGG AGTGTGGGCT GGTGCTTGAC AAGACCTGTT TTTATGCAGA ACAGGGAGGG 
CAGACCTATG ATGAGGGCTA CATGGTTAAA GAGGAGGAGA GCAGTGAAGA TAAAACAGAG 
TTTACAGTGA GAAACACTCA GGTTCGGGGA GGTTACGTGC TGCACATGGG TACATTGTAC 
GGGCACTTGA AAGTTGGAGA TCAAGTGCGT CTATACGTTG ATGAGCCCAA GCGTCGTCCT 
ATTATGAGTA ACCACACAGC CACTCACATC CTGAACTTTG CCTTGCGCTC TGTTCTTGGA 
GAAGCAGATC AACGGGGTTC ATTGGTTGCC CCAGACAGGC TAAGATTTGA CTTCACAGCT 
AAAGGTGCAA TGTCCACCCA GGAGATCCGC AAAACCGAAG AGATTGCAAA TGACATTATC 
AAGGAAAATA AGGTTGTGTA TGCACTAGAT TGCTCTCTCG CAGAAGCAAA AGCTATCCAG 
GGTCTTCGTG CAGTATTTGA TGAAACATAC CCAGATCCTG TGCGTGTTGT GTCTATAGGA 
ATTCCTGTGG AAGATCTGCT GGCTGACCCA TCTAGTCCTG CTGGGTCTAT AACTTCTGTG 
GAGTTCTGTG GAGGAACGCA TCTCCAAAAT TCTGGCCATG CTGGTCCTTT CATTATTGTT 
ACAGAGGAAG CCATTGCCAA AGGAATTCGC AGAATTGTTG CTGTCACAGG TGCAGAGGCA 
CAAAAGGCCT TAAGGAGACA GGATGCCCTG AAAGTTAATC TTTCTGATCT GGAGAAGAAA 
GTGAAGAAAC AGAATTCTCC TAACAAGGAT GTACAAAAGG AAATAGCTGA CCTTAGTGAG 
AATCTCGCAA CAGCATCCAT TGCACAGTGG CAGAAGGATG TACTAAGGGA GCAGCTGAAA 
ACCTTGAAGA AAACTATGGA TGACCTGGAT CGTGCCAGCA AAGCAGATGT TCAGAAACGG 
GTATTGGAAA TAACCAAACA ACTCATAGAG GAACATCCTA ATCAGCCACT ACTTGTACTT 
GAGATGGAGA ATGGAGCATC TGCAAAGGCT TTGAACGAGT CCCTGAAACT TCTCAAGTCT 
CAGTCTCCCC ATACTGCTGC AATGATCTTC TCTGTAGATA ACGATGCAGG AAAGATCACG 
TGTTTATGCC AGGTGCCCCA GGACACTGCA GACAAAGGTC TGAAGGCCAA TGAGTGGGTG 
CAGCAAGTAT CTGATTTGAT GGATGGCAGG GGAGGAGGAA AAGACACCTC TGCCCAAGCA 
ACTGGAAAGA ATGTGGGGTG CCTGTCCCAA GTCCTAAAGC TAGCTGAAGA TTTTGCCAAA 
ATGAAACTGG AGTCCATGAA AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

aars ( XM_002931661.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002931707.2 Xenopus tropicalis alanyl-tRNA synthetase 1 (aars1), transcript variant X2, mRNA.

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CloneID	OXa56967
Clone ID Related Accession (Same CDS sequence)	XM_004914051.4 , XM_002931661.5
Accession Version	XM_002931661.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alanine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931661.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACGT GTATGACAGC CTCTGAAATC CGGCAGAAGT TTATTGACTT CTTTAAAGAA 
AATGCCCACA CATATGTCCA TTCTTCTGCC ACAGTCCCCC TGGATGACCC AACACTGCTC 
TTCGCCAATG CTGGAATGAA CCAGTTTAAG CCAATCTTCC TGAATACCAT TGATCCTTCT 
CATCCAATGG CAAAGCTAAA CCGAGCAGCT AATACACAGA AGTGCATCCG TGCTGGAGGC 
AAGCACAATG ATCTCGATGA TGTGGGCAAA GATGTCTATC ACCACACATT TTTTGAGATG 
CTTGGATCAT GGTCCTTTGG GGATTATTTC AAGGAGCTCG CCTGTAAACT GGCACTGGAC 
CTGCTAACCA AGGAGTTTGG CATTGACATA GAGCGCTTGT ATGTCACTTA TTTTGGCGGT 
AATGAGGCTG CTGGTCTAGA GCCTGACTTG GAATGTAAAC AAATATGGCT GAACTTGGGC 
CTGCCAGAGA GTCGTATCCT TCCAGGAAGC ATGAAGGACA ACTTTTGGGA AATGGGTGAT 
ACGGGTCCTT GTGGTCCCTG TAGTGAGATT CATTTTGACC GAATTGGGGG CCGTGATGCT 
GCCCATCTTG TGAATATGGA TGATCCAAAT GTCCTTGAGA TCTGGAACCT GGTGTTCATC 
CAGTTTAATA GGGAAACTGA TGGAACCTTA AAGCCACTGC CTAAGAAGAG CATTGATACT 
GGAATGGGAC TGGAAAGACT TGTGTCTGTG TTGCAAAATA AGATGTCCAA CTATGATACA 
GATCTGTTTG TTCCATACTT TGAAGCTATC GAAAAGGGCA CAGGTGCAAG ACCCTACACT 
GGGAAAGTGG GAGCTGAGGA TGTTGATGGC ATAGACATGG CATACAGAGT GTTGGCTGAT 
CATGCTAGAA CAATTACTGT GGCACTTGCT GATGGAGGAA GACCAGATAA CACTGGACGT 
GGATATGTTT TACGCCGCAT CCTTCGCCGT GCTGTGAGAT ACGCCCATGA GAAACTTAAT 
GCATCTAAAG GATTTTTTGC CACTCTGGTT GATGTGGTTG TCCAGTCACT GGGAAATGCT 
TTTCCAGAGC TTAAAAAAGA TCCTGATATG GTCAAAGACA TCATTAATGA AGAGGAAACA 
CAGTTCCTGA AAACACTTAA CAGGGGTCGT CGCATCTTGG ACCGAAAGAT AAACAGTCTG 
GGAGAACACA AAGTTATCCC TGGTGACACT GCCTGGCTGC TGTACGACAC CTACGGCTTC 
CCAGTGGATT TGACTAGTTT GATTGCTGAG GAACGAGGAC TGACATTGGA CTTGGAGAGC 
TTTGAAGAGG AGCGTAAAGC TGCTCAGCTT AAATCTCAAG GAAAGGGTAC AGGTGACGAA 
GACCTTCTCA TGCTGGACAT CTATGCAATT GAAGAACTGC GTTCCCAGGG ACTTGAACCC 
ACTGATGATC TGCCAAAATA CAAATATACA TCTAATGCTG ATGGTGTTTA CGAATTTGAG 
AGTTTGATAG CAACAGTGAA AGCAATCCGC AGAGATAAGA TGTTTGTGGA AGAGGCGAGC 
ACTGGACAGG AGTGTGGGCT GGTGCTTGAC AAGACCTGTT TTTATGCAGA ACAGGGAGGG 
CAGACCTATG ATGAGGGCTA CATGGTTAAA GAGGAGGAGA GCAGTGAAGA TAAAACAGAG 
TTTACAGTGA GAAACACTCA GGTTCGGGGA GGTTACGTGC TGCACATGGG TACATTGTAC 
GGGCACTTGA AAGTTGGAGA TCAAGTGCGT CTATACGTTG ATGAGCCCAA GCGTCGTCCT 
ATTATGAGTA ACCACACAGC CACTCACATC CTGAACTTTG CCTTGCGCTC TGTTCTTGGA 
GAAGCAGATC AACGGGGTTC ATTGGTTGCC CCAGACAGGC TAAGATTTGA CTTCACAGCT 
AAAGGTGCAA TGTCCACCCA GGAGATCCGC AAAACCGAAG AGATTGCAAA TGACATTATC 
AAGGAAAATA AGGTTGTGTA TGCACTAGAT TGCTCTCTCG CAGAAGCAAA AGCTATCCAG 
GGTCTTCGTG CAGTATTTGA TGAAACATAC CCAGATCCTG TGCGTGTTGT GTCTATAGGA 
ATTCCTGTGG AAGATCTGCT GGCTGACCCA TCTAGTCCTG CTGGGTCTAT AACTTCTGTG 
GAGTTCTGTG GAGGAACGCA TCTCCAAAAT TCTGGCCATG CTGGTCCTTT CATTATTGTT 
ACAGAGGAAG CCATTGCCAA AGGAATTCGC AGAATTGTTG CTGTCACAGG TGCAGAGGCA 
CAAAAGGCCT TAAGGAGACA GGATGCCCTG AAAGTTAATC TTTCTGATCT GGAGAAGAAA 
GTGAAGAAAC AGAATTCTCC TAACAAGGAT GTACAAAAGG AAATAGCTGA CCTTAGTGAG 
AATCTCGCAA CAGCATCCAT TGCACAGTGG CAGAAGGATG TACTAAGGGA GCAGCTGAAA 
ACCTTGAAGA AAACTATGGA TGACCTGGAT CGTGCCAGCA AAGCAGATGT TCAGAAACGG 
GTATTGGAAA TAACCAAACA ACTCATAGAG GAACATCCTA ATCAGCCACT ACTTGTACTT 
GAGATGGAGA ATGGAGCATC TGCAAAGGCT TTGAACGAGT CCCTGAAACT TCTCAAGTCT 
CAGTCTCCCC ATACTGCTGC AATGATCTTC TCTGTAGATA ACGATGCAGG AAAGATCACG 
TGTTTATGCC AGGTGCCCCA GGACACTGCA GACAAAGGTC TGAAGGCCAA TGAGTGGGTG 
CAGCAAGTAT CTGATTTGAT GGATGGCAGG GGAGGAGGAA AAGACACCTC TGCCCAAGCA 
ACTGGAAAGA ATGTGGGGTG CCTGTCCCAA GTCCTAAAGC TAGCTGAAGA TTTTGCCAAA 
ATGAAACTGG AGTCCATGAA AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002931707.2
CDS	131..3037
Translation	MKTCMTASEIRQKFIDFFKENAHTYVHSSATVPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLNRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKLALDLLTKEFGIDIERLYVTYFGGNEAAGLEPDLECKQIWLNLGLPESRILPGSMKDNFWEMGDTGPCGPCSEIHFDRIGGRDAAHLVNMDDPNVLEIWNLVFIQFNRETDGTLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIEKGTGARPYTGKVGAEDVDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASKGFFATLVDVVVQSLGNAFPELKKDPDMVKDIINEEETQFLKTLNRGRRILDRKINSLGEHKVIPGDTAWLLYDTYGFPVDLTSLIAEERGLTLDLESFEEERKAAQLKSQGKGTGDEDLLMLDIYAIEELRSQGLEPTDDLPKYKYTSNADGVYEFESLIATVKAIRRDKMFVEEASTGQECGLVLDKTCFYAEQGGQTYDEGYMVKEEESSEDKTEFTVRNTQVRGGYVLHMGTLYGHLKVGDQVRLYVDEPKRRPIMSNHTATHILNFALRSVLGEADQRGSLVAPDRLRFDFTAKGAMSTQEIRKTEEIANDIIKENKVVYALDCSLAEAKAIQGLRAVFDETYPDPVRVVSIGIPVEDLLADPSSPAGSITSVEFCGGTHLQNSGHAGPFIIVTEEAIAKGIRRIVAVTGAEAQKALRRQDALKVNLSDLEKKVKKQNSPNKDVQKEIADLSENLATASIAQWQKDVLREQLKTLKKTMDDLDRASKADVQKRVLEITKQLIEEHPNQPLLVLEMENGASAKALNESLKLLKSQSPHTAAMIFSVDNDAGKITCLCQVPQDTADKGLKANEWVQQVSDLMDGRGGGKDTSAQATGKNVGCLSQVLKLAEDFAKMKLESMKN

Target ORF information:

RefSeq Version	XM_002931661.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis alanyl-tRNA synthetase 1 (aars1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002931661.5

ORF Insert Sequence:

ATGAAGACGT GTATGACAGC CTCTGAAATC CGGCAGAAGT TTATTGACTT CTTTAAAGAA 
AATGCCCACA CATATGTCCA TTCTTCTGCC ACAGTCCCCC TGGATGACCC AACACTGCTC 
TTCGCCAATG CTGGAATGAA CCAGTTTAAG CCAATCTTCC TGAATACCAT TGATCCTTCT 
CATCCAATGG CAAAGCTAAA CCGAGCAGCT AATACACAGA AGTGCATCCG TGCTGGAGGC 
AAGCACAATG ATCTCGATGA TGTGGGCAAA GATGTCTATC ACCACACATT TTTTGAGATG 
CTTGGATCAT GGTCCTTTGG GGATTATTTC AAGGAGCTCG CCTGTAAACT GGCACTGGAC 
CTGCTAACCA AGGAGTTTGG CATTGACATA GAGCGCTTGT ATGTCACTTA TTTTGGCGGT 
AATGAGGCTG CTGGTCTAGA GCCTGACTTG GAATGTAAAC AAATATGGCT GAACTTGGGC 
CTGCCAGAGA GTCGTATCCT TCCAGGAAGC ATGAAGGACA ACTTTTGGGA AATGGGTGAT 
ACGGGTCCTT GTGGTCCCTG TAGTGAGATT CATTTTGACC GAATTGGGGG CCGTGATGCT 
GCCCATCTTG TGAATATGGA TGATCCAAAT GTCCTTGAGA TCTGGAACCT GGTGTTCATC 
CAGTTTAATA GGGAAACTGA TGGAACCTTA AAGCCACTGC CTAAGAAGAG CATTGATACT 
GGAATGGGAC TGGAAAGACT TGTGTCTGTG TTGCAAAATA AGATGTCCAA CTATGATACA 
GATCTGTTTG TTCCATACTT TGAAGCTATC GAAAAGGGCA CAGGTGCAAG ACCCTACACT 
GGGAAAGTGG GAGCTGAGGA TGTTGATGGC ATAGACATGG CATACAGAGT GTTGGCTGAT 
CATGCTAGAA CAATTACTGT GGCACTTGCT GATGGAGGAA GACCAGATAA CACTGGACGT 
GGATATGTTT TACGCCGCAT CCTTCGCCGT GCTGTGAGAT ACGCCCATGA GAAACTTAAT 
GCATCTAAAG GATTTTTTGC CACTCTGGTT GATGTGGTTG TCCAGTCACT GGGAAATGCT 
TTTCCAGAGC TTAAAAAAGA TCCTGATATG GTCAAAGACA TCATTAATGA AGAGGAAACA 
CAGTTCCTGA AAACACTTAA CAGGGGTCGT CGCATCTTGG ACCGAAAGAT AAACAGTCTG 
GGAGAACACA AAGTTATCCC TGGTGACACT GCCTGGCTGC TGTACGACAC CTACGGCTTC 
CCAGTGGATT TGACTAGTTT GATTGCTGAG GAACGAGGAC TGACATTGGA CTTGGAGAGC 
TTTGAAGAGG AGCGTAAAGC TGCTCAGCTT AAATCTCAAG GAAAGGGTAC AGGTGACGAA 
GACCTTCTCA TGCTGGACAT CTATGCAATT GAAGAACTGC GTTCCCAGGG ACTTGAACCC 
ACTGATGATC TGCCAAAATA CAAATATACA TCTAATGCTG ATGGTGTTTA CGAATTTGAG 
AGTTTGATAG CAACAGTGAA AGCAATCCGC AGAGATAAGA TGTTTGTGGA AGAGGCGAGC 
ACTGGACAGG AGTGTGGGCT GGTGCTTGAC AAGACCTGTT TTTATGCAGA ACAGGGAGGG 
CAGACCTATG ATGAGGGCTA CATGGTTAAA GAGGAGGAGA GCAGTGAAGA TAAAACAGAG 
TTTACAGTGA GAAACACTCA GGTTCGGGGA GGTTACGTGC TGCACATGGG TACATTGTAC 
GGGCACTTGA AAGTTGGAGA TCAAGTGCGT CTATACGTTG ATGAGCCCAA GCGTCGTCCT 
ATTATGAGTA ACCACACAGC CACTCACATC CTGAACTTTG CCTTGCGCTC TGTTCTTGGA 
GAAGCAGATC AACGGGGTTC ATTGGTTGCC CCAGACAGGC TAAGATTTGA CTTCACAGCT 
AAAGGTGCAA TGTCCACCCA GGAGATCCGC AAAACCGAAG AGATTGCAAA TGACATTATC 
AAGGAAAATA AGGTTGTGTA TGCACTAGAT TGCTCTCTCG CAGAAGCAAA AGCTATCCAG 
GGTCTTCGTG CAGTATTTGA TGAAACATAC CCAGATCCTG TGCGTGTTGT GTCTATAGGA 
ATTCCTGTGG AAGATCTGCT GGCTGACCCA TCTAGTCCTG CTGGGTCTAT AACTTCTGTG 
GAGTTCTGTG GAGGAACGCA TCTCCAAAAT TCTGGCCATG CTGGTCCTTT CATTATTGTT 
ACAGAGGAAG CCATTGCCAA AGGAATTCGC AGAATTGTTG CTGTCACAGG TGCAGAGGCA 
CAAAAGGCCT TAAGGAGACA GGATGCCCTG AAAGTTAATC TTTCTGATCT GGAGAAGAAA 
GTGAAGAAAC AGAATTCTCC TAACAAGGAT GTACAAAAGG AAATAGCTGA CCTTAGTGAG 
AATCTCGCAA CAGCATCCAT TGCACAGTGG CAGAAGGATG TACTAAGGGA GCAGCTGAAA 
ACCTTGAAGA AAACTATGGA TGACCTGGAT CGTGCCAGCA AAGCAGATGT TCAGAAACGG 
GTATTGGAAA TAACCAAACA ACTCATAGAG GAACATCCTA ATCAGCCACT ACTTGTACTT 
GAGATGGAGA ATGGAGCATC TGCAAAGGCT TTGAACGAGT CCCTGAAACT TCTCAAGTCT 
CAGTCTCCCC ATACTGCTGC AATGATCTTC TCTGTAGATA ACGATGCAGG AAAGATCACG 
TGTTTATGCC AGGTGCCCCA GGACACTGCA GACAAAGGTC TGAAGGCCAA TGAGTGGGTG 
CAGCAAGTAT CTGATTTGAT GGATGGCAGG GGAGGAGGAA AAGACACCTC TGCCCAAGCA 
ACTGGAAAGA ATGTGGGGTG CCTGTCCCAA GTCCTAAAGC TAGCTGAAGA TTTTGCCAAA 
ATGAAACTGG AGTCCATGAA AAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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aars cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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