LOC100492280 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100492280
Entrez Gene ID	100492280
Full Name	prolyl 4-hydroxylase subunit alpha-1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004915787.3	XP_004915844.1	prolyl 4-hydroxylase subunit alpha-1 isoform X1
XM_004915788.4	XP_004915845.1	prolyl 4-hydroxylase subunit alpha-1 isoform X2
XM_004915788.3	XP_004915845.1	prolyl 4-hydroxylase subunit alpha-1 isoform X2
XM_002935967.5	XP_002936013.2	prolyl 4-hydroxylase subunit alpha-1 isoform X1
XM_002935967.4	XP_002936013.2	prolyl 4-hydroxylase subunit alpha-1 isoform X1
XM_004915787.4	XP_004915844.1	prolyl 4-hydroxylase subunit alpha-1 isoform X1
XM_031905487.1	XP_031761347.1	prolyl 4-hydroxylase subunit alpha-1 isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	P4HA1	XP_005619064.1
Bos taurus (cattle)	P4HA1	NP_001069238.1
Danio rerio (zebrafish)	p4ha1a	NP_001038463.1
Drosophila melanogaster (fruit fly)	PH4alphaEFB	NP_733371.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000971	XP_309164.4
Rattus norvegicus (Norway rat)	P4ha1	NP_742059.2
Caenorhabditis elegans (roundworm)	dpy-18	NP_499464.1
Xenopus tropicalis (tropical clawed frog)	LOC100490484	XP_002936004.1
	P4HA1	XP_001103971.2
Xenopus tropicalis (tropical clawed frog)	p4ha1	XP_004915844.1
Homo sapiens (human)	P4HA1	NP_001136067.1
Mus musculus (house mouse)	P4ha1	NP_035160.1
Danio rerio (zebrafish)	LOC556879	XP_005156147.1
Caenorhabditis elegans (roundworm)	phy-2	NP_502317.1
Pan troglodytes (chimpanzee)	P4HA1	XP_001140652.1
Gallus gallus (chicken)	P4HA1	NP_001185667.1

Gene Ontology

The following LOC100492280 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100492280 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa12571	XM_004915787.4 Latest version!	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa12571	XM_002935967.5 Latest version!	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa12572	XM_004915788.4 Latest version!	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OXa61841	XM_031905487.1 Latest version!	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OXa12572	XM_004915788.3	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa12571	XM_002935967.4	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa12571	XM_004915787.3	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100492280 ( XM_004915787.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915844.1 Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa12571
Clone ID Related Accession (Same CDS sequence)	XM_004915787.3 , XM_002935967.5 , XM_002935967.4 , XM_004915787.4
Accession Version	XM_004915787.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915787.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG GCGAGCCACC 
ATTTCAAACC CTATCACAGG AGTCCTGGAG ACAGCTCAAT ACAGAATTAG CAAAAGTGCC 
TGGCTGTCTG CGTATGAAGA TCCAGTGGTC GCACGTCTCA ACCGAAGAAT CGAAGGAGTA 
ACAGGACTGG ACATGTCCAC AGCAGAAGAA TTACAGGTTG CAAATTATGG AATGGGAGGA 
CAATATGAAC CACACTTTGA CTTTGCACGG AAAGATGAGC CAGATGCCTT TAAAGAACTT 
GGAACAGGCA ACAGAGTTGC TACTTGGCTA TTTTATATGA GCGATGTGGA AGCAGGTGGC 
GCTACAGTAT TTCCAGAAGT AGGCGCAGCT GTATATCCCA AAAAGGGGAC AGCGGTCTTC 
TGGTACAATC TTTTTGAGAG CGGTGAAGGT GATTACAGCA CAAGGCATGC AGCCTGCCCT 
GTATTAGTTG GAAATAAATG GGTGTCCAAT AAATGGATAC ATGAGCGGGG TCAGGAGTTC 
CGAAGGCCGT GTAATTTATC AGAGTTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915844.1
CDS	469..2061
Translation	MKIHLVLPLLGLFSCAHADFFTSIGHMTDLLNTEKDLVTSLKDYIKAEEQKLAQIKKWADKLDHLTDTATKDPEGFLGHPVNAFKLMKRLNTEWVKLENLILKDISDGFISNLTIQRQYFPNDEDQTGAAKALLRLQDTYNLDTDTISKGNLPGVKHKTSLTAEDCFDLGKTAYTDADYYHTELWMEQALSQLDAGEESSLDKVMVLDYLSYAVYQQGDLDKALTLTKLLLELDPEHQRGNGNLRYFEYIMSKESNKSSSSPSEGAELGTRKGRPKDHLPERQKYEKLCRGEGVKMTSRRQKRLFCRYFDGNKDPLLILSPTKQEDEWDKPRIVRYHDIISDEEISKVKELAKPRLRRATISNPITGVLETAQYRISKSAWLSAYEDPVVARLNRRIEGVTGLDMSTAEELQVANYGMGGQYEPHFDFARKDEPDAFKELGTGNRVATWLFYMSDVEAGGATVFPEVGAAVYPKKGTAVFWYNLFESGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEFRRPCNLSELE

Target ORF information:

RefSeq Version	XM_004915787.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915787.3

ORF Insert Sequence:

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG GCGAGCCACC 
ATTTCAAACC CTATCACAGG AGTCCTGGAG ACAGCTCAAT ACAGAATTAG CAAAAGTGCC 
TGGCTGTCTG CGTATGAAGA TCCAGTGGTC GCACGTCTCA ACCGAAGAAT CGAAGGAGTA 
ACAGGACTGG ACATGTCCAC AGCAGAAGAA TTACAGGTTG CAAATTATGG AATGGGAGGA 
CAATATGAAC CACACTTTGA CTTTGCACGG AAAGATGAGC CAGATGCCTT TAAAGAACTT 
GGAACAGGCA ACAGAGTTGC TACTTGGCTA TTTTATATGA GCGATGTGGA AGCAGGTGGC 
GCTACAGTAT TTCCAGAAGT AGGCGCAGCT GTATATCCCA AAAAGGGGAC AGCGGTCTTC 
TGGTACAATC TTTTTGAGAG CGGTGAAGGT GATTACAGCA CAAGGCATGC AGCCTGCCCT 
GTATTAGTTG GAAATAAATG GGTGTCCAAT AAATGGATAC ATGAGCGGGG TCAGGAGTTC 
CGAAGGCCGT GTAATTTATC AGAGTTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492280 ( XM_002935967.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936013.2 Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa12571
Clone ID Related Accession (Same CDS sequence)	XM_004915787.3 , XM_002935967.5 , XM_002935967.4 , XM_004915787.4
Accession Version	XM_002935967.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935967.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG GCGAGCCACC 
ATTTCAAACC CTATCACAGG AGTCCTGGAG ACAGCTCAAT ACAGAATTAG CAAAAGTGCC 
TGGCTGTCTG CGTATGAAGA TCCAGTGGTC GCACGTCTCA ACCGAAGAAT CGAAGGAGTA 
ACAGGACTGG ACATGTCCAC AGCAGAAGAA TTACAGGTTG CAAATTATGG AATGGGAGGA 
CAATATGAAC CACACTTTGA CTTTGCACGG AAAGATGAGC CAGATGCCTT TAAAGAACTT 
GGAACAGGCA ACAGAGTTGC TACTTGGCTA TTTTATATGA GCGATGTGGA AGCAGGTGGC 
GCTACAGTAT TTCCAGAAGT AGGCGCAGCT GTATATCCCA AAAAGGGGAC AGCGGTCTTC 
TGGTACAATC TTTTTGAGAG CGGTGAAGGT GATTACAGCA CAAGGCATGC AGCCTGCCCT 
GTATTAGTTG GAAATAAATG GGTGTCCAAT AAATGGATAC ATGAGCGGGG TCAGGAGTTC 
CGAAGGCCGT GTAATTTATC AGAGTTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936013.2
CDS	478..2070
Translation	MKIHLVLPLLGLFSCAHADFFTSIGHMTDLLNTEKDLVTSLKDYIKAEEQKLAQIKKWADKLDHLTDTATKDPEGFLGHPVNAFKLMKRLNTEWVKLENLILKDISDGFISNLTIQRQYFPNDEDQTGAAKALLRLQDTYNLDTDTISKGNLPGVKHKTSLTAEDCFDLGKTAYTDADYYHTELWMEQALSQLDAGEESSLDKVMVLDYLSYAVYQQGDLDKALTLTKLLLELDPEHQRGNGNLRYFEYIMSKESNKSSSSPSEGAELGTRKGRPKDHLPERQKYEKLCRGEGVKMTSRRQKRLFCRYFDGNKDPLLILSPTKQEDEWDKPRIVRYHDIISDEEISKVKELAKPRLRRATISNPITGVLETAQYRISKSAWLSAYEDPVVARLNRRIEGVTGLDMSTAEELQVANYGMGGQYEPHFDFARKDEPDAFKELGTGNRVATWLFYMSDVEAGGATVFPEVGAAVYPKKGTAVFWYNLFESGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEFRRPCNLSELE

Target ORF information:

RefSeq Version	XM_002935967.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935967.4

ORF Insert Sequence:

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG GCGAGCCACC 
ATTTCAAACC CTATCACAGG AGTCCTGGAG ACAGCTCAAT ACAGAATTAG CAAAAGTGCC 
TGGCTGTCTG CGTATGAAGA TCCAGTGGTC GCACGTCTCA ACCGAAGAAT CGAAGGAGTA 
ACAGGACTGG ACATGTCCAC AGCAGAAGAA TTACAGGTTG CAAATTATGG AATGGGAGGA 
CAATATGAAC CACACTTTGA CTTTGCACGG AAAGATGAGC CAGATGCCTT TAAAGAACTT 
GGAACAGGCA ACAGAGTTGC TACTTGGCTA TTTTATATGA GCGATGTGGA AGCAGGTGGC 
GCTACAGTAT TTCCAGAAGT AGGCGCAGCT GTATATCCCA AAAAGGGGAC AGCGGTCTTC 
TGGTACAATC TTTTTGAGAG CGGTGAAGGT GATTACAGCA CAAGGCATGC AGCCTGCCCT 
GTATTAGTTG GAAATAAATG GGTGTCCAAT AAATGGATAC ATGAGCGGGG TCAGGAGTTC 
CGAAGGCCGT GTAATTTATC AGAGTTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492280 ( XM_004915788.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915845.1 Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa12572
Clone ID Related Accession (Same CDS sequence)	XM_004915788.4 , XM_004915788.3
Accession Version	XM_004915788.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004915788.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG ACGGGCTACA 
GTGCATGACC CTCAAACAGG AAAGTTGACC ACTGCTCAAT ACAGAGTCTC TAAGAGTGCC 
TGGCTGTCTG CGTATGAAGA TCCAGTGGTC GCACGTCTCA ACCGAAGAAT CGAAGGAGTA 
ACAGGACTGG ACATGTCCAC AGCAGAAGAA TTACAGGTTG CAAATTATGG AATGGGAGGA 
CAATATGAAC CACACTTTGA CTTTGCACGG AAAGATGAGC CAGATGCCTT TAAAGAACTT 
GGAACAGGCA ACAGAGTTGC TACTTGGCTA TTTTATATGA GCGATGTGGA AGCAGGTGGC 
GCTACAGTAT TTCCAGAAGT AGGCGCAGCT GTATATCCCA AAAAGGGGAC AGCGGTCTTC 
TGGTACAATC TTTTTGAGAG CGGTGAAGGT GATTACAGCA CAAGGCATGC AGCCTGCCCT 
GTATTAGTTG GAAATAAATG GGTGTCCAAT AAATGGATAC ATGAGCGGGG TCAGGAGTTC 
CGAAGGCCGT GTAATTTATC AGAGTTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915845.1
CDS	479..2071
Translation	MKIHLVLPLLGLFSCAHADFFTSIGHMTDLLNTEKDLVTSLKDYIKAEEQKLAQIKKWADKLDHLTDTATKDPEGFLGHPVNAFKLMKRLNTEWVKLENLILKDISDGFISNLTIQRQYFPNDEDQTGAAKALLRLQDTYNLDTDTISKGNLPGVKHKTSLTAEDCFDLGKTAYTDADYYHTELWMEQALSQLDAGEESSLDKVMVLDYLSYAVYQQGDLDKALTLTKLLLELDPEHQRGNGNLRYFEYIMSKESNKSSSSPSEGAELGTRKGRPKDHLPERQKYEKLCRGEGVKMTSRRQKRLFCRYFDGNKDPLLILSPTKQEDEWDKPRIVRYHDIISDEEISKVKELAKPRLRRATVHDPQTGKLTTAQYRVSKSAWLSAYEDPVVARLNRRIEGVTGLDMSTAEELQVANYGMGGQYEPHFDFARKDEPDAFKELGTGNRVATWLFYMSDVEAGGATVFPEVGAAVYPKKGTAVFWYNLFESGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEFRRPCNLSELE

Target ORF information:

RefSeq Version	XM_004915788.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915788.3

ORF Insert Sequence:

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG ACGGGCTACA 
GTGCATGACC CTCAAACAGG AAAGTTGACC ACTGCTCAAT ACAGAGTCTC TAAGAGTGCC 
TGGCTGTCTG CGTATGAAGA TCCAGTGGTC GCACGTCTCA ACCGAAGAAT CGAAGGAGTA 
ACAGGACTGG ACATGTCCAC AGCAGAAGAA TTACAGGTTG CAAATTATGG AATGGGAGGA 
CAATATGAAC CACACTTTGA CTTTGCACGG AAAGATGAGC CAGATGCCTT TAAAGAACTT 
GGAACAGGCA ACAGAGTTGC TACTTGGCTA TTTTATATGA GCGATGTGGA AGCAGGTGGC 
GCTACAGTAT TTCCAGAAGT AGGCGCAGCT GTATATCCCA AAAAGGGGAC AGCGGTCTTC 
TGGTACAATC TTTTTGAGAG CGGTGAAGGT GATTACAGCA CAAGGCATGC AGCCTGCCCT 
GTATTAGTTG GAAATAAATG GGTGTCCAAT AAATGGATAC ATGAGCGGGG TCAGGAGTTC 
CGAAGGCCGT GTAATTTATC AGAGTTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492280 ( XM_002935967.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936013.2 Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X1, mRNA.

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CloneID	OXa12571
Clone ID Related Accession (Same CDS sequence)	XM_004915787.3 , XM_002935967.5 , XM_002935967.4 , XM_004915787.4
Accession Version	XM_002935967.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935967.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG GCGAGCCACC 
ATTTCAAACC CTATCACAGG AGTCCTGGAG ACAGCTCAAT ACAGAATTAG CAAAAGTGCC 
TGGCTGTCTG CGTATGAAGA TCCAGTGGTC GCACGTCTCA ACCGAAGAAT CGAAGGAGTA 
ACAGGACTGG ACATGTCCAC AGCAGAAGAA TTACAGGTTG CAAATTATGG AATGGGAGGA 
CAATATGAAC CACACTTTGA CTTTGCACGG AAAGATGAGC CAGATGCCTT TAAAGAACTT 
GGAACAGGCA ACAGAGTTGC TACTTGGCTA TTTTATATGA GCGATGTGGA AGCAGGTGGC 
GCTACAGTAT TTCCAGAAGT AGGCGCAGCT GTATATCCCA AAAAGGGGAC AGCGGTCTTC 
TGGTACAATC TTTTTGAGAG CGGTGAAGGT GATTACAGCA CAAGGCATGC AGCCTGCCCT 
GTATTAGTTG GAAATAAATG GGTGTCCAAT AAATGGATAC ATGAGCGGGG TCAGGAGTTC 
CGAAGGCCGT GTAATTTATC AGAGTTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936013.2
CDS	402..1994
Translation	MKIHLVLPLLGLFSCAHADFFTSIGHMTDLLNTEKDLVTSLKDYIKAEEQKLAQIKKWADKLDHLTDTATKDPEGFLGHPVNAFKLMKRLNTEWVKLENLILKDISDGFISNLTIQRQYFPNDEDQTGAAKALLRLQDTYNLDTDTISKGNLPGVKHKTSLTAEDCFDLGKTAYTDADYYHTELWMEQALSQLDAGEESSLDKVMVLDYLSYAVYQQGDLDKALTLTKLLLELDPEHQRGNGNLRYFEYIMSKESNKSSSSPSEGAELGTRKGRPKDHLPERQKYEKLCRGEGVKMTSRRQKRLFCRYFDGNKDPLLILSPTKQEDEWDKPRIVRYHDIISDEEISKVKELAKPRLRRATISNPITGVLETAQYRISKSAWLSAYEDPVVARLNRRIEGVTGLDMSTAEELQVANYGMGGQYEPHFDFARKDEPDAFKELGTGNRVATWLFYMSDVEAGGATVFPEVGAAVYPKKGTAVFWYNLFESGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEFRRPCNLSELE

Target ORF information:

RefSeq Version	XM_002935967.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935967.5

ORF Insert Sequence:

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG GCGAGCCACC 
ATTTCAAACC CTATCACAGG AGTCCTGGAG ACAGCTCAAT ACAGAATTAG CAAAAGTGCC 
TGGCTGTCTG CGTATGAAGA TCCAGTGGTC GCACGTCTCA ACCGAAGAAT CGAAGGAGTA 
ACAGGACTGG ACATGTCCAC AGCAGAAGAA TTACAGGTTG CAAATTATGG AATGGGAGGA 
CAATATGAAC CACACTTTGA CTTTGCACGG AAAGATGAGC CAGATGCCTT TAAAGAACTT 
GGAACAGGCA ACAGAGTTGC TACTTGGCTA TTTTATATGA GCGATGTGGA AGCAGGTGGC 
GCTACAGTAT TTCCAGAAGT AGGCGCAGCT GTATATCCCA AAAAGGGGAC AGCGGTCTTC 
TGGTACAATC TTTTTGAGAG CGGTGAAGGT GATTACAGCA CAAGGCATGC AGCCTGCCCT 
GTATTAGTTG GAAATAAATG GGTGTCCAAT AAATGGATAC ATGAGCGGGG TCAGGAGTTC 
CGAAGGCCGT GTAATTTATC AGAGTTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492280 ( XM_004915787.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915844.1 Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X3, mRNA.

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CloneID	OXa12571
Clone ID Related Accession (Same CDS sequence)	XM_004915787.3 , XM_002935967.5 , XM_002935967.4 , XM_004915787.4
Accession Version	XM_004915787.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915787.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG GCGAGCCACC 
ATTTCAAACC CTATCACAGG AGTCCTGGAG ACAGCTCAAT ACAGAATTAG CAAAAGTGCC 
TGGCTGTCTG CGTATGAAGA TCCAGTGGTC GCACGTCTCA ACCGAAGAAT CGAAGGAGTA 
ACAGGACTGG ACATGTCCAC AGCAGAAGAA TTACAGGTTG CAAATTATGG AATGGGAGGA 
CAATATGAAC CACACTTTGA CTTTGCACGG AAAGATGAGC CAGATGCCTT TAAAGAACTT 
GGAACAGGCA ACAGAGTTGC TACTTGGCTA TTTTATATGA GCGATGTGGA AGCAGGTGGC 
GCTACAGTAT TTCCAGAAGT AGGCGCAGCT GTATATCCCA AAAAGGGGAC AGCGGTCTTC 
TGGTACAATC TTTTTGAGAG CGGTGAAGGT GATTACAGCA CAAGGCATGC AGCCTGCCCT 
GTATTAGTTG GAAATAAATG GGTGTCCAAT AAATGGATAC ATGAGCGGGG TCAGGAGTTC 
CGAAGGCCGT GTAATTTATC AGAGTTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915844.1
CDS	392..1984
Translation	MKIHLVLPLLGLFSCAHADFFTSIGHMTDLLNTEKDLVTSLKDYIKAEEQKLAQIKKWADKLDHLTDTATKDPEGFLGHPVNAFKLMKRLNTEWVKLENLILKDISDGFISNLTIQRQYFPNDEDQTGAAKALLRLQDTYNLDTDTISKGNLPGVKHKTSLTAEDCFDLGKTAYTDADYYHTELWMEQALSQLDAGEESSLDKVMVLDYLSYAVYQQGDLDKALTLTKLLLELDPEHQRGNGNLRYFEYIMSKESNKSSSSPSEGAELGTRKGRPKDHLPERQKYEKLCRGEGVKMTSRRQKRLFCRYFDGNKDPLLILSPTKQEDEWDKPRIVRYHDIISDEEISKVKELAKPRLRRATISNPITGVLETAQYRISKSAWLSAYEDPVVARLNRRIEGVTGLDMSTAEELQVANYGMGGQYEPHFDFARKDEPDAFKELGTGNRVATWLFYMSDVEAGGATVFPEVGAAVYPKKGTAVFWYNLFESGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEFRRPCNLSELE

Target ORF information:

RefSeq Version	XM_004915787.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915787.4

ORF Insert Sequence:

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG GCGAGCCACC 
ATTTCAAACC CTATCACAGG AGTCCTGGAG ACAGCTCAAT ACAGAATTAG CAAAAGTGCC 
TGGCTGTCTG CGTATGAAGA TCCAGTGGTC GCACGTCTCA ACCGAAGAAT CGAAGGAGTA 
ACAGGACTGG ACATGTCCAC AGCAGAAGAA TTACAGGTTG CAAATTATGG AATGGGAGGA 
CAATATGAAC CACACTTTGA CTTTGCACGG AAAGATGAGC CAGATGCCTT TAAAGAACTT 
GGAACAGGCA ACAGAGTTGC TACTTGGCTA TTTTATATGA GCGATGTGGA AGCAGGTGGC 
GCTACAGTAT TTCCAGAAGT AGGCGCAGCT GTATATCCCA AAAAGGGGAC AGCGGTCTTC 
TGGTACAATC TTTTTGAGAG CGGTGAAGGT GATTACAGCA CAAGGCATGC AGCCTGCCCT 
GTATTAGTTG GAAATAAATG GGTGTCCAAT AAATGGATAC ATGAGCGGGG TCAGGAGTTC 
CGAAGGCCGT GTAATTTATC AGAGTTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492280 ( XM_004915788.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004915845.1 Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa12572
Clone ID Related Accession (Same CDS sequence)	XM_004915788.4 , XM_004915788.3
Accession Version	XM_004915788.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004915788.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG ACGGGCTACA 
GTGCATGACC CTCAAACAGG AAAGTTGACC ACTGCTCAAT ACAGAGTCTC TAAGAGTGCC 
TGGCTGTCTG CGTATGAAGA TCCAGTGGTC GCACGTCTCA ACCGAAGAAT CGAAGGAGTA 
ACAGGACTGG ACATGTCCAC AGCAGAAGAA TTACAGGTTG CAAATTATGG AATGGGAGGA 
CAATATGAAC CACACTTTGA CTTTGCACGG AAAGATGAGC CAGATGCCTT TAAAGAACTT 
GGAACAGGCA ACAGAGTTGC TACTTGGCTA TTTTATATGA GCGATGTGGA AGCAGGTGGC 
GCTACAGTAT TTCCAGAAGT AGGCGCAGCT GTATATCCCA AAAAGGGGAC AGCGGTCTTC 
TGGTACAATC TTTTTGAGAG CGGTGAAGGT GATTACAGCA CAAGGCATGC AGCCTGCCCT 
GTATTAGTTG GAAATAAATG GGTGTCCAAT AAATGGATAC ATGAGCGGGG TCAGGAGTTC 
CGAAGGCCGT GTAATTTATC AGAGTTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004915845.1
CDS	403..1995
Translation	MKIHLVLPLLGLFSCAHADFFTSIGHMTDLLNTEKDLVTSLKDYIKAEEQKLAQIKKWADKLDHLTDTATKDPEGFLGHPVNAFKLMKRLNTEWVKLENLILKDISDGFISNLTIQRQYFPNDEDQTGAAKALLRLQDTYNLDTDTISKGNLPGVKHKTSLTAEDCFDLGKTAYTDADYYHTELWMEQALSQLDAGEESSLDKVMVLDYLSYAVYQQGDLDKALTLTKLLLELDPEHQRGNGNLRYFEYIMSKESNKSSSSPSEGAELGTRKGRPKDHLPERQKYEKLCRGEGVKMTSRRQKRLFCRYFDGNKDPLLILSPTKQEDEWDKPRIVRYHDIISDEEISKVKELAKPRLRRATVHDPQTGKLTTAQYRVSKSAWLSAYEDPVVARLNRRIEGVTGLDMSTAEELQVANYGMGGQYEPHFDFARKDEPDAFKELGTGNRVATWLFYMSDVEAGGATVFPEVGAAVYPKKGTAVFWYNLFESGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEFRRPCNLSELE

Target ORF information:

RefSeq Version	XM_004915788.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004915788.4

ORF Insert Sequence:

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG ACGGGCTACA 
GTGCATGACC CTCAAACAGG AAAGTTGACC ACTGCTCAAT ACAGAGTCTC TAAGAGTGCC 
TGGCTGTCTG CGTATGAAGA TCCAGTGGTC GCACGTCTCA ACCGAAGAAT CGAAGGAGTA 
ACAGGACTGG ACATGTCCAC AGCAGAAGAA TTACAGGTTG CAAATTATGG AATGGGAGGA 
CAATATGAAC CACACTTTGA CTTTGCACGG AAAGATGAGC CAGATGCCTT TAAAGAACTT 
GGAACAGGCA ACAGAGTTGC TACTTGGCTA TTTTATATGA GCGATGTGGA AGCAGGTGGC 
GCTACAGTAT TTCCAGAAGT AGGCGCAGCT GTATATCCCA AAAAGGGGAC AGCGGTCTTC 
TGGTACAATC TTTTTGAGAG CGGTGAAGGT GATTACAGCA CAAGGCATGC AGCCTGCCCT 
GTATTAGTTG GAAATAAATG GGTGTCCAAT AAATGGATAC ATGAGCGGGG TCAGGAGTTC 
CGAAGGCCGT GTAATTTATC AGAGTTGGAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492280 ( XM_031905487.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031761347.1 Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X4, mRNA.

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CloneID	OXa61841
Clone ID Related Accession (Same CDS sequence)	XM_031905487.1
Accession Version	XM_031905487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	prolyl 4-hydroxylase subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG GCGAGCCACC 
ATTTCAAACC CTATCACAGG AGTCCTGGAG ACAGCTCAAT ACAGAATTAG CAAAAGACGG 
GCTACAGTGC ATGACCCTCA AACAGGAAAG TTGACCACTG CTCAATACAG AGTCTCTAAG 
AGTGCCTGGC TGTCTGCGTA TGAAGATCCA GTGGTCGCAC GTCTCAACCG AAGAATCGAA 
GGAGTAACAG GACTGGACAT GTCCACAGCA GAAGAATTAC AGGTTGCAAA TTATGGAATG 
GGAGGACAAT ATGAACCACA CTTTGACTTT GCACGGAAAG ATGAGCCAGA TGCCTTTAAA 
GAACTTGGAA CAGGCAACAG AGTTGCTACT TGGCTATTTT ATATGAGCGA TGTGGAAGCA 
GGTGGCGCTA CAGTATTTCC AGAAGTAGGC GCAGCTGTAT ATCCCAAAAA GGGGACAGCG 
GTCTTCTGGT ACAATCTTTT TGAGAGCGGT GAAGGTGATT ACAGCACAAG GCATGCAGCC 
TGCCCTGTAT TAGTTGGAAA TAAATGGGTG TCCAATAAAT GGATACATGA GCGGGGTCAG 
GAGTTCCGAA GGCCGTGTAA TTTATCAGAG TTGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031761347.1
CDS	404..2062
Translation	MKIHLVLPLLGLFSCAHADFFTSIGHMTDLLNTEKDLVTSLKDYIKAEEQKLAQIKKWADKLDHLTDTATKDPEGFLGHPVNAFKLMKRLNTEWVKLENLILKDISDGFISNLTIQRQYFPNDEDQTGAAKALLRLQDTYNLDTDTISKGNLPGVKHKTSLTAEDCFDLGKTAYTDADYYHTELWMEQALSQLDAGEESSLDKVMVLDYLSYAVYQQGDLDKALTLTKLLLELDPEHQRGNGNLRYFEYIMSKESNKSSSSPSEGAELGTRKGRPKDHLPERQKYEKLCRGEGVKMTSRRQKRLFCRYFDGNKDPLLILSPTKQEDEWDKPRIVRYHDIISDEEISKVKELAKPRLRRATISNPITGVLETAQYRISKRRATVHDPQTGKLTTAQYRVSKSAWLSAYEDPVVARLNRRIEGVTGLDMSTAEELQVANYGMGGQYEPHFDFARKDEPDAFKELGTGNRVATWLFYMSDVEAGGATVFPEVGAAVYPKKGTAVFWYNLFESGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEFRRPCNLSELE

Target ORF information:

RefSeq Version	XM_031905487.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prolyl 4-hydroxylase subunit alpha-1 (LOC100492280), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031905487.1

ORF Insert Sequence:

ATGAAAATTC ACCTAGTATT GCCCCTCTTG GGGCTTTTCT CTTGTGCACA CGCAGACTTC 
TTCACCTCAA TTGGTCACAT GACAGATTTG CTAAACACAG AAAAGGACTT GGTTACGTCA 
CTGAAAGATT ATATTAAGGC AGAGGAGCAG AAATTGGCAC AGATCAAAAA ATGGGCAGAT 
AAATTAGATC ATCTGACAGA CACTGCAACA AAGGACCCAG AGGGATTCTT GGGACATCCA 
GTGAATGCCT TCAAGCTGAT GAAGAGGCTC AACACAGAAT GGGTTAAATT AGAGAATTTG 
ATACTTAAAG ATATATCTGA TGGATTTATC TCTAACTTAA CTATTCAGCG CCAGTATTTC 
CCGAATGATG AGGACCAGAC TGGTGCTGCC AAAGCTCTAC TTCGTTTGCA GGACACTTAT 
AACCTTGACA CAGATACTAT CTCTAAGGGA AACCTTCCAG GTGTTAAGCA CAAAACATCA 
TTAACAGCTG AAGACTGTTT TGATTTGGGT AAAACTGCTT ACACAGATGC TGACTACTAC 
CACACTGAGC TATGGATGGA GCAAGCTTTG AGCCAGTTGG ATGCAGGAGA AGAGTCTTCT 
CTTGACAAGG TCATGGTCCT AGATTATCTT AGTTATGCTG TGTACCAACA AGGGGACTTG 
GACAAAGCGC TGACCCTAAC CAAACTGTTA CTAGAGCTGG ATCCTGAGCA TCAAAGAGGG 
AATGGGAATC TGAGATACTT TGAATATATC ATGTCAAAAG AAAGTAATAA ATCTAGCTCT 
AGCCCTTCCG AGGGTGCAGA ACTCGGAACA AGGAAAGGTC GGCCCAAAGA TCACCTTCCT 
GAGAGACAGA AATATGAGAA GCTGTGTCGT GGAGAGGGTG TCAAAATGAC TTCCCGCAGA 
CAAAAGAGAC TTTTCTGCCG GTATTTTGAT GGTAACAAAG ATCCATTGCT CATCCTGTCT 
CCCACAAAGC AAGAAGATGA ATGGGACAAG CCACGCATTG TGCGCTACCA TGATATTATT 
TCTGATGAGG AGATTTCAAA GGTTAAGGAG TTAGCAAAGC CAAGACTGAG GCGAGCCACC 
ATTTCAAACC CTATCACAGG AGTCCTGGAG ACAGCTCAAT ACAGAATTAG CAAAAGACGG 
GCTACAGTGC ATGACCCTCA AACAGGAAAG TTGACCACTG CTCAATACAG AGTCTCTAAG 
AGTGCCTGGC TGTCTGCGTA TGAAGATCCA GTGGTCGCAC GTCTCAACCG AAGAATCGAA 
GGAGTAACAG GACTGGACAT GTCCACAGCA GAAGAATTAC AGGTTGCAAA TTATGGAATG 
GGAGGACAAT ATGAACCACA CTTTGACTTT GCACGGAAAG ATGAGCCAGA TGCCTTTAAA 
GAACTTGGAA CAGGCAACAG AGTTGCTACT TGGCTATTTT ATATGAGCGA TGTGGAAGCA 
GGTGGCGCTA CAGTATTTCC AGAAGTAGGC GCAGCTGTAT ATCCCAAAAA GGGGACAGCG 
GTCTTCTGGT ACAATCTTTT TGAGAGCGGT GAAGGTGATT ACAGCACAAG GCATGCAGCC 
TGCCCTGTAT TAGTTGGAAA TAAATGGGTG TCCAATAAAT GGATACATGA GCGGGGTCAG 
GAGTTCCGAA GGCCGTGTAA TTTATCAGAG TTGGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100492280 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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