LOC100492173 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004911963.3	XP_004912020.1	fibronectin type-III domain-containing protein 3A isoform X1
XM_018091725.1	XP_017947214.1	fibronectin type-III domain-containing protein 3A isoform X1
XM_004911963.4	XP_004912020.2	fibronectin type-III domain-containing protein 3A isoform X1
XM_018091725.2	XP_017947214.2	fibronectin type-III domain-containing protein 3A isoform X1
XM_031897219.1	XP_031753079.1	fibronectin type-III domain-containing protein 3A isoform X2
XM_002935746.5	XP_002935792.3	fibronectin type-III domain-containing protein 3A isoform X3
XM_004911964.3	XP_004912021.1	fibronectin type-III domain-containing protein 3A isoform X1
XM_002935746.4	XP_002935792.2	fibronectin type-III domain-containing protein 3A isoform X2
XM_018091724.2	XP_017947213.2	fibronectin type-III domain-containing protein 3A isoform X1
XM_018091724.1	XP_017947213.1	fibronectin type-III domain-containing protein 3A isoform X1
XM_004911964.4	XP_004912021.2	fibronectin type-III domain-containing protein 3A isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa52761	XM_004911963.4 Latest version!	Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52761	XM_018091725.2 Latest version!	Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52761	XM_004911964.4 Latest version!	Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52761	XM_018091724.2 Latest version!	Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52762	XM_031897219.1 Latest version!	Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa52763	XM_002935746.5 Latest version!	Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa06143	XM_018091724.1 Latest version!	Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa06143	XM_018091725.1 Latest version!	Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa06143	XM_004911963.3 Latest version!	Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa06143	XM_004911964.3 Latest version!	Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa06144	XM_002935746.4 Latest version!	Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

LOC100492173 ( XM_004911963.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912020.2 Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa52761
Clone ID Related Accession (Same CDS sequence)	XM_004911963.4 , XM_018091725.2 , XM_018091724.2 , XM_004911964.4
Accession Version	XM_004911963.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	fibronectin type-III domain-containing protein 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911963.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGACAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912020.2
CDS	722..4330
Translation	MYVLSVMADHPPPPLDSTPLLTTEIPLLPAPIVNGEGGQQVILVQVNPGEAFTIRREDGQFQCITGPAQVPMMSPNGSVPPIYVPPGYAQQVIEDNGVRRVVVVPQPPEFHTGSHAVIHRPPPPIPGFIPLPAMMPPPPRHIYSPVTGAGDVASQYTPQYHAAQVYGDLDTLSTHGRSNLRDERSNKIHERLQKKLKDRHGVQKEKLNSPPFSPKKSTSPINESNGLVKGQNSGLNTGQTKNKHKGKGGSPGETEIQEKDDETKALEALLSTVSQPVVSDIQARSVTLSWSSPSSLISEQNDGTCSPEPYAYEILLSSSGKDGKYKVAYSGEKATVTMDDLRPATDYHARVQAESNCVKGNLSEAVSFTTLCCEPDTPNNPRITNRTKNSLTLQWKASCDNGCKIENYLLEWDEGKGSKEFCQCYYGPQKQFRLTKLSPAMGYTFRLAAKNDMGMSGFSEEVFFYTSGSAPTMPASPTLNKAGISWLSMQWTKPSGTPSDEGISYILEMEDETSGYGFKPIYNGDDLTFTVRNLRRSTKYKFRVIAYNTEGKSSPSEVVEFVTCPDRPGAPSKPSVKGKVHAHNFRIVWDPPKDNGGAAITKYVMEMSEDSNGNKWDVVYSGTAREYLCDHLSPGSSYRLQVHCISDGGKSQVSDTLLVQTPAVFPGPCQPPRLQGRPRAREVQMKWAPPQLDGGSPVTCYSLEMSSSDASEHREVYQGPELECTVASLLPGKAYSFRVRAANKTGFGPFSEKCEMTTAPGPPDQCRPPQLTCRSATCVLIHWEAPNNNGAEIMEYRVEWGGMEGCMQICYCGPGFNHEVKGLSPAIVYYCRVQAVNIAGAGHFSDVVACLTPASVPAAVTSLWGVKEDEVNSSNYCPSTCLAIQWEKPSDHGSEITTYSIDYGDKQPITVYKATSYILDNLQPDTTYRIRVQALNSLGAGPFSHSVKLKTKPLPPEPPRLECAVYSHQNLKLKWGDGASRALPTDSIQYHLQMEDKNERFVSLYRGPCQTYKVQRLNESTSYTFRIQACNEAGEGPFSSEYVFTTPKSLPAALKAPRIDRINEHTCDVTWESLQPMKGDPIIYILQLMVGKDSDFKQVYKGPSTSFRHSALQLNCEYRFRVCAIRQCQDSAGHQDLIGPYSATVLFISHRSETQTSTNKDTVETTRTTRTLSDEQCAAVILVLFATFSILIAFIIQYFVIK

Target ORF information:

RefSeq Version	XM_004911963.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911963.4

ORF Insert Sequence:

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGACAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492173 ( XM_018091725.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947214.2 Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa52761
Clone ID Related Accession (Same CDS sequence)	XM_004911963.4 , XM_018091725.2 , XM_018091724.2 , XM_004911964.4
Accession Version	XM_018091725.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	fibronectin type-III domain-containing protein 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGACAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947214.2
CDS	100..3708
Translation	MYVLSVMADHPPPPLDSTPLLTTEIPLLPAPIVNGEGGQQVILVQVNPGEAFTIRREDGQFQCITGPAQVPMMSPNGSVPPIYVPPGYAQQVIEDNGVRRVVVVPQPPEFHTGSHAVIHRPPPPIPGFIPLPAMMPPPPRHIYSPVTGAGDVASQYTPQYHAAQVYGDLDTLSTHGRSNLRDERSNKIHERLQKKLKDRHGVQKEKLNSPPFSPKKSTSPINESNGLVKGQNSGLNTGQTKNKHKGKGGSPGETEIQEKDDETKALEALLSTVSQPVVSDIQARSVTLSWSSPSSLISEQNDGTCSPEPYAYEILLSSSGKDGKYKVAYSGEKATVTMDDLRPATDYHARVQAESNCVKGNLSEAVSFTTLCCEPDTPNNPRITNRTKNSLTLQWKASCDNGCKIENYLLEWDEGKGSKEFCQCYYGPQKQFRLTKLSPAMGYTFRLAAKNDMGMSGFSEEVFFYTSGSAPTMPASPTLNKAGISWLSMQWTKPSGTPSDEGISYILEMEDETSGYGFKPIYNGDDLTFTVRNLRRSTKYKFRVIAYNTEGKSSPSEVVEFVTCPDRPGAPSKPSVKGKVHAHNFRIVWDPPKDNGGAAITKYVMEMSEDSNGNKWDVVYSGTAREYLCDHLSPGSSYRLQVHCISDGGKSQVSDTLLVQTPAVFPGPCQPPRLQGRPRAREVQMKWAPPQLDGGSPVTCYSLEMSSSDASEHREVYQGPELECTVASLLPGKAYSFRVRAANKTGFGPFSEKCEMTTAPGPPDQCRPPQLTCRSATCVLIHWEAPNNNGAEIMEYRVEWGGMEGCMQICYCGPGFNHEVKGLSPAIVYYCRVQAVNIAGAGHFSDVVACLTPASVPAAVTSLWGVKEDEVNSSNYCPSTCLAIQWEKPSDHGSEITTYSIDYGDKQPITVYKATSYILDNLQPDTTYRIRVQALNSLGAGPFSHSVKLKTKPLPPEPPRLECAVYSHQNLKLKWGDGASRALPTDSIQYHLQMEDKNERFVSLYRGPCQTYKVQRLNESTSYTFRIQACNEAGEGPFSSEYVFTTPKSLPAALKAPRIDRINEHTCDVTWESLQPMKGDPIIYILQLMVGKDSDFKQVYKGPSTSFRHSALQLNCEYRFRVCAIRQCQDSAGHQDLIGPYSATVLFISHRSETQTSTNKDTVETTRTTRTLSDEQCAAVILVLFATFSILIAFIIQYFVIK

Target ORF information:

RefSeq Version	XM_018091725.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091725.2

ORF Insert Sequence:

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGACAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492173 ( XM_018091724.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947213.2 Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X3, mRNA.

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CloneID	OXa52761
Clone ID Related Accession (Same CDS sequence)	XM_004911963.4 , XM_018091725.2 , XM_018091724.2 , XM_004911964.4
Accession Version	XM_018091724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	fibronectin type-III domain-containing protein 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGACAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947213.2
CDS	194..3802
Translation	MYVLSVMADHPPPPLDSTPLLTTEIPLLPAPIVNGEGGQQVILVQVNPGEAFTIRREDGQFQCITGPAQVPMMSPNGSVPPIYVPPGYAQQVIEDNGVRRVVVVPQPPEFHTGSHAVIHRPPPPIPGFIPLPAMMPPPPRHIYSPVTGAGDVASQYTPQYHAAQVYGDLDTLSTHGRSNLRDERSNKIHERLQKKLKDRHGVQKEKLNSPPFSPKKSTSPINESNGLVKGQNSGLNTGQTKNKHKGKGGSPGETEIQEKDDETKALEALLSTVSQPVVSDIQARSVTLSWSSPSSLISEQNDGTCSPEPYAYEILLSSSGKDGKYKVAYSGEKATVTMDDLRPATDYHARVQAESNCVKGNLSEAVSFTTLCCEPDTPNNPRITNRTKNSLTLQWKASCDNGCKIENYLLEWDEGKGSKEFCQCYYGPQKQFRLTKLSPAMGYTFRLAAKNDMGMSGFSEEVFFYTSGSAPTMPASPTLNKAGISWLSMQWTKPSGTPSDEGISYILEMEDETSGYGFKPIYNGDDLTFTVRNLRRSTKYKFRVIAYNTEGKSSPSEVVEFVTCPDRPGAPSKPSVKGKVHAHNFRIVWDPPKDNGGAAITKYVMEMSEDSNGNKWDVVYSGTAREYLCDHLSPGSSYRLQVHCISDGGKSQVSDTLLVQTPAVFPGPCQPPRLQGRPRAREVQMKWAPPQLDGGSPVTCYSLEMSSSDASEHREVYQGPELECTVASLLPGKAYSFRVRAANKTGFGPFSEKCEMTTAPGPPDQCRPPQLTCRSATCVLIHWEAPNNNGAEIMEYRVEWGGMEGCMQICYCGPGFNHEVKGLSPAIVYYCRVQAVNIAGAGHFSDVVACLTPASVPAAVTSLWGVKEDEVNSSNYCPSTCLAIQWEKPSDHGSEITTYSIDYGDKQPITVYKATSYILDNLQPDTTYRIRVQALNSLGAGPFSHSVKLKTKPLPPEPPRLECAVYSHQNLKLKWGDGASRALPTDSIQYHLQMEDKNERFVSLYRGPCQTYKVQRLNESTSYTFRIQACNEAGEGPFSSEYVFTTPKSLPAALKAPRIDRINEHTCDVTWESLQPMKGDPIIYILQLMVGKDSDFKQVYKGPSTSFRHSALQLNCEYRFRVCAIRQCQDSAGHQDLIGPYSATVLFISHRSETQTSTNKDTVETTRTTRTLSDEQCAAVILVLFATFSILIAFIIQYFVIK

Target ORF information:

RefSeq Version	XM_018091724.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091724.2

ORF Insert Sequence:

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGACAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492173 ( XM_004911964.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912021.2 Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X2, mRNA.

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CloneID	OXa52761
Clone ID Related Accession (Same CDS sequence)	XM_004911963.4 , XM_018091725.2 , XM_018091724.2 , XM_004911964.4
Accession Version	XM_004911964.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	fibronectin type-III domain-containing protein 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911964.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGACAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912021.2
CDS	630..4238
Translation	MYVLSVMADHPPPPLDSTPLLTTEIPLLPAPIVNGEGGQQVILVQVNPGEAFTIRREDGQFQCITGPAQVPMMSPNGSVPPIYVPPGYAQQVIEDNGVRRVVVVPQPPEFHTGSHAVIHRPPPPIPGFIPLPAMMPPPPRHIYSPVTGAGDVASQYTPQYHAAQVYGDLDTLSTHGRSNLRDERSNKIHERLQKKLKDRHGVQKEKLNSPPFSPKKSTSPINESNGLVKGQNSGLNTGQTKNKHKGKGGSPGETEIQEKDDETKALEALLSTVSQPVVSDIQARSVTLSWSSPSSLISEQNDGTCSPEPYAYEILLSSSGKDGKYKVAYSGEKATVTMDDLRPATDYHARVQAESNCVKGNLSEAVSFTTLCCEPDTPNNPRITNRTKNSLTLQWKASCDNGCKIENYLLEWDEGKGSKEFCQCYYGPQKQFRLTKLSPAMGYTFRLAAKNDMGMSGFSEEVFFYTSGSAPTMPASPTLNKAGISWLSMQWTKPSGTPSDEGISYILEMEDETSGYGFKPIYNGDDLTFTVRNLRRSTKYKFRVIAYNTEGKSSPSEVVEFVTCPDRPGAPSKPSVKGKVHAHNFRIVWDPPKDNGGAAITKYVMEMSEDSNGNKWDVVYSGTAREYLCDHLSPGSSYRLQVHCISDGGKSQVSDTLLVQTPAVFPGPCQPPRLQGRPRAREVQMKWAPPQLDGGSPVTCYSLEMSSSDASEHREVYQGPELECTVASLLPGKAYSFRVRAANKTGFGPFSEKCEMTTAPGPPDQCRPPQLTCRSATCVLIHWEAPNNNGAEIMEYRVEWGGMEGCMQICYCGPGFNHEVKGLSPAIVYYCRVQAVNIAGAGHFSDVVACLTPASVPAAVTSLWGVKEDEVNSSNYCPSTCLAIQWEKPSDHGSEITTYSIDYGDKQPITVYKATSYILDNLQPDTTYRIRVQALNSLGAGPFSHSVKLKTKPLPPEPPRLECAVYSHQNLKLKWGDGASRALPTDSIQYHLQMEDKNERFVSLYRGPCQTYKVQRLNESTSYTFRIQACNEAGEGPFSSEYVFTTPKSLPAALKAPRIDRINEHTCDVTWESLQPMKGDPIIYILQLMVGKDSDFKQVYKGPSTSFRHSALQLNCEYRFRVCAIRQCQDSAGHQDLIGPYSATVLFISHRSETQTSTNKDTVETTRTTRTLSDEQCAAVILVLFATFSILIAFIIQYFVIK

Target ORF information:

RefSeq Version	XM_004911964.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911964.4

ORF Insert Sequence:

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGACAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492173 ( XM_031897219.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031753079.1 Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa52762
Clone ID Related Accession (Same CDS sequence)	XM_031897219.1
Accession Version	XM_031897219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	fibronectin type-III domain-containing protein 3A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCAA CAGTTATTCT GGTGCAAGTT AATCCAGGGG AGGCATTTAC AATAAGAAGA 
GAAGATGGGC AGTTTCAGTG CATCACAGGC CCTGCTCAGG TTCCAATGAT GTCACCAAAT 
GGCTCTGTGC CCCCAATATA TGTGCCTCCA GGATACGCCC AGCAGGTAAT TGAGGATAAT 
GGTGTGCGTA GAGTTGTAGT GGTTCCACAA CCCCCTGAGT TCCATACAGG CAGCCATGCG 
GTCATACATC GACCCCCACC TCCAATACCA GGATTCATTC CTCTTCCAGC TATGATGCCT 
CCCCCACCTC GACACATCTA CTCACCAGTG ACTGGAGCAG GAGATGTGGC CTCCCAGTAT 
ACCCCACAGT ATCATGCCGC ACAGGTATAT GGAGATCTCG ATACTCTTTC TACGCATGGA 
CGATCTAACC TCAGAGATGA AAGATCCAAC AAAATACATG AGAGGTTGCA GAAAAAGCTC 
AAAGACAGAC ATGGAGTACA GAAAGAGAAG CTTAACAGCC CCCCATTCTC ACCCAAGAAA 
AGCACTTCTC CAATAAATGA ATCAAATGGA CTTGTAAAAG GCCAGAATTC AGGCTTAAAT 
ACAGGGCAGA CAAAGAACAA GCACAAAGGG AAAGGTGGCT CTCCAGGGGA GACAGAAATA 
CAAGAAAAAG ATGATGAAAC AAAGGCACTC GAAGCACTGC TCTCAACGGT TTCCCAACCT 
GTGGTATCCG ATATCCAGGC CAGGTCTGTA ACATTATCAT GGTCATCACC TTCCAGCCTT 
ATCAGTGAGC AGAATGATGG GACATGTAGC CCTGAACCAT ACGCTTATGA AATCCTGCTG 
TCGAGCAGTG GGAAAGATGG AAAGTACAAG GTTGCTTATA GTGGAGAAAA GGCCACTGTT 
ACTATGGATG ACTTGAGACC TGCAACTGAT TATCATGCAC GAGTTCAAGC GGAGTCGAAC 
TGTGTAAAGG GAAACTTGTC AGAAGCGGTC AGTTTCACTA CACTTTGCTG TGAACCAGAC 
ACACCAAATA ATCCACGAAT AACTAATCGG ACCAAAAATT CACTCACTCT CCAGTGGAAG 
GCATCATGTG ACAATGGCTG TAAAATAGAG AACTATCTTC TGGAATGGGA TGAAGGGAAA 
GGGAGCAAAG AGTTCTGCCA GTGTTATTAT GGACCACAGA AGCAGTTTAG GTTAACAAAG 
TTGTCACCAG CTATGGGATA CACATTCAGA CTTGCAGCAA AAAATGATAT GGGCATGAGT 
GGTTTCAGTG AAGAAGTTTT CTTTTACACC TCAGGCAGTG CTCCTACCAT GCCAGCAAGT 
CCGACACTAA ATAAAGCAGG AATTAGTTGG TTATCCATGC AGTGGACTAA ACCCTCAGGT 
ACACCATCTG ATGAAGGAAT TTCTTATATT TTGGAAATGG AAGATGAAAC TTCGGGATAT 
GGATTTAAGC CAATATACAA TGGTGATGAC CTTACATTTA CAGTGAGAAA TCTGAGAAGA 
AGTACAAAGT ATAAGTTTAG GGTCATTGCA TATAACACTG AAGGCAAGAG CAGCCCAAGT 
GAGGTGGTGG AGTTTGTGAC TTGTCCAGAC AGACCAGGAG CACCCTCAAA GCCTTCTGTC 
AAGGGAAAGG TCCATGCACA TAACTTCAGG ATAGTATGGG ATCCACCTAA GGACAATGGA 
GGAGCAGCAA TCACAAAATA CGTTATGGAG ATGTCTGAAG ACAGCAATGG AAACAAGTGG 
GATGTCGTAT ACAGTGGAAC AGCTCGAGAA TACCTGTGTG ACCATCTGAG TCCAGGCTCT 
TCTTATCGCT TACAAGTCCA TTGCATCAGT GATGGAGGGA AGAGTCAGGT ATCAGATACA 
CTGTTAGTTC AGACTCCAGC GGTGTTCCCT GGGCCATGCC AGCCCCCCAG GCTCCAAGGC 
AGACCAAGAG CAAGAGAAGT GCAAATGAAA TGGGCACCAC CGCAGCTTGA TGGTGGTTCC 
CCAGTCACGT GCTACAGCCT GGAGATGTCG TCTTCTGATG CCAGTGAGCA TAGAGAAGTC 
TACCAAGGAC CTGAACTGGA ATGCACAGTG GCCAGTCTTC TTCCAGGGAA GGCGTACAGC 
TTTCGAGTGC GCGCTGCAAA CAAAACTGGG TTTGGTCCAT TCTCTGAAAA GTGTGAAATG 
ACAACAGCTC CAGGACCACC AGATCAATGT AGACCTCCTC AATTAACATG CAGATCTGCA 
ACCTGTGTCC TTATACACTG GGAGGCCCCC AACAACAATG GAGCTGAGAT TATGGAGTAC 
CGGGTTGAAT GGGGAGGCAT GGAAGGGTGC ATGCAGATCT GCTACTGCGG GCCTGGTTTT 
AATCATGAAG TTAAAGGCCT TTCGCCAGCA ATTGTTTATT ACTGCAGGGT TCAGGCTGTA 
AATATTGCTG GTGCGGGCCA TTTTAGTGAT GTGGTTGCAT GTTTAACGCC GGCATCGGTC 
CCCGCTGCTG TGACAAGTTT GTGGGGAGTA AAAGAAGATG AAGTAAACAG TTCAAATTAC 
TGTCCTTCCA CTTGCCTTGC AATACAGTGG GAAAAACCAT CTGATCATGG TTCAGAGATT 
ACCACTTACA GCATAGACTA TGGGGACAAG CAGCCGATTA CTGTTTACAA AGCAACAAGC 
TACATTCTAG ATAACTTACA GCCAGACACA ACATACAGAA TACGGGTTCA AGCCCTAAAC 
AGCTTGGGAG CTGGTCCTTT CAGCCATTCT GTGAAACTGA AAACCAAGCC ACTACCCCCA 
GAACCACCTC GACTGGAATG TGCGGTGTAC AGTCACCAGA ACCTTAAGCT GAAGTGGGGA 
GACGGTGCAT CCAGGGCATT ACCCACAGAT TCCATTCAGT ATCACCTTCA AATGGAGGAT 
AAGAATGAAA GATTTGTATC CTTATATAGA GGACCATGTC AAACATACAA AGTACAAAGG 
CTCAATGAGT CCACGTCATA CACATTTCGT ATTCAGGCAT GCAATGAGGC TGGTGAGGGG 
CCTTTTTCCT CGGAATATGT TTTTACAACT CCAAAATCAC TTCCAGCTGC CTTGAAAGCT 
CCAAGAATTG ACCGAATTAA TGAACATACC TGTGATGTCA CGTGGGAGTC CTTACAGCCA 
ATGAAAGGAG ATCCGATTAT CTACATTTTG CAATTAATGG TTGGAAAAGA CTCTGATTTT 
AAACAGGTTT ATAAGGGACC TAGTACATCA TTCCGCCACT CTGCCCTCCA GCTAAACTGT 
GAATATCGTT TCCGTGTATG TGCCATTCGC CAGTGCCAAG ATTCTGCAGG ACATCAGGAT 
CTCATTGGTC CATACAGTGC CACCGTCCTC TTCATTTCTC ACCGAAGTGA AACCCAAACC 
AGCACCAACA AAGATACTGT GGAAACCACA AGGACAACAC GGACGCTTAG TGACGAGCAG 
TGCGCTGCTG TCATCCTTGT GCTCTTTGCT ACGTTCTCCA TTCTGATTGC CTTTATCATC 
CAGTACTTTG TTATCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031753079.1
CDS	79..3579
Translation	MRATVILVQVNPGEAFTIRREDGQFQCITGPAQVPMMSPNGSVPPIYVPPGYAQQVIEDNGVRRVVVVPQPPEFHTGSHAVIHRPPPPIPGFIPLPAMMPPPPRHIYSPVTGAGDVASQYTPQYHAAQVYGDLDTLSTHGRSNLRDERSNKIHERLQKKLKDRHGVQKEKLNSPPFSPKKSTSPINESNGLVKGQNSGLNTGQTKNKHKGKGGSPGETEIQEKDDETKALEALLSTVSQPVVSDIQARSVTLSWSSPSSLISEQNDGTCSPEPYAYEILLSSSGKDGKYKVAYSGEKATVTMDDLRPATDYHARVQAESNCVKGNLSEAVSFTTLCCEPDTPNNPRITNRTKNSLTLQWKASCDNGCKIENYLLEWDEGKGSKEFCQCYYGPQKQFRLTKLSPAMGYTFRLAAKNDMGMSGFSEEVFFYTSGSAPTMPASPTLNKAGISWLSMQWTKPSGTPSDEGISYILEMEDETSGYGFKPIYNGDDLTFTVRNLRRSTKYKFRVIAYNTEGKSSPSEVVEFVTCPDRPGAPSKPSVKGKVHAHNFRIVWDPPKDNGGAAITKYVMEMSEDSNGNKWDVVYSGTAREYLCDHLSPGSSYRLQVHCISDGGKSQVSDTLLVQTPAVFPGPCQPPRLQGRPRAREVQMKWAPPQLDGGSPVTCYSLEMSSSDASEHREVYQGPELECTVASLLPGKAYSFRVRAANKTGFGPFSEKCEMTTAPGPPDQCRPPQLTCRSATCVLIHWEAPNNNGAEIMEYRVEWGGMEGCMQICYCGPGFNHEVKGLSPAIVYYCRVQAVNIAGAGHFSDVVACLTPASVPAAVTSLWGVKEDEVNSSNYCPSTCLAIQWEKPSDHGSEITTYSIDYGDKQPITVYKATSYILDNLQPDTTYRIRVQALNSLGAGPFSHSVKLKTKPLPPEPPRLECAVYSHQNLKLKWGDGASRALPTDSIQYHLQMEDKNERFVSLYRGPCQTYKVQRLNESTSYTFRIQACNEAGEGPFSSEYVFTTPKSLPAALKAPRIDRINEHTCDVTWESLQPMKGDPIIYILQLMVGKDSDFKQVYKGPSTSFRHSALQLNCEYRFRVCAIRQCQDSAGHQDLIGPYSATVLFISHRSETQTSTNKDTVETTRTTRTLSDEQCAAVILVLFATFSILIAFIIQYFVIK

Target ORF information:

RefSeq Version	XM_031897219.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031897219.1

ORF Insert Sequence:

ATGCGAGCAA CAGTTATTCT GGTGCAAGTT AATCCAGGGG AGGCATTTAC AATAAGAAGA 
GAAGATGGGC AGTTTCAGTG CATCACAGGC CCTGCTCAGG TTCCAATGAT GTCACCAAAT 
GGCTCTGTGC CCCCAATATA TGTGCCTCCA GGATACGCCC AGCAGGTAAT TGAGGATAAT 
GGTGTGCGTA GAGTTGTAGT GGTTCCACAA CCCCCTGAGT TCCATACAGG CAGCCATGCG 
GTCATACATC GACCCCCACC TCCAATACCA GGATTCATTC CTCTTCCAGC TATGATGCCT 
CCCCCACCTC GACACATCTA CTCACCAGTG ACTGGAGCAG GAGATGTGGC CTCCCAGTAT 
ACCCCACAGT ATCATGCCGC ACAGGTATAT GGAGATCTCG ATACTCTTTC TACGCATGGA 
CGATCTAACC TCAGAGATGA AAGATCCAAC AAAATACATG AGAGGTTGCA GAAAAAGCTC 
AAAGACAGAC ATGGAGTACA GAAAGAGAAG CTTAACAGCC CCCCATTCTC ACCCAAGAAA 
AGCACTTCTC CAATAAATGA ATCAAATGGA CTTGTAAAAG GCCAGAATTC AGGCTTAAAT 
ACAGGGCAGA CAAAGAACAA GCACAAAGGG AAAGGTGGCT CTCCAGGGGA GACAGAAATA 
CAAGAAAAAG ATGATGAAAC AAAGGCACTC GAAGCACTGC TCTCAACGGT TTCCCAACCT 
GTGGTATCCG ATATCCAGGC CAGGTCTGTA ACATTATCAT GGTCATCACC TTCCAGCCTT 
ATCAGTGAGC AGAATGATGG GACATGTAGC CCTGAACCAT ACGCTTATGA AATCCTGCTG 
TCGAGCAGTG GGAAAGATGG AAAGTACAAG GTTGCTTATA GTGGAGAAAA GGCCACTGTT 
ACTATGGATG ACTTGAGACC TGCAACTGAT TATCATGCAC GAGTTCAAGC GGAGTCGAAC 
TGTGTAAAGG GAAACTTGTC AGAAGCGGTC AGTTTCACTA CACTTTGCTG TGAACCAGAC 
ACACCAAATA ATCCACGAAT AACTAATCGG ACCAAAAATT CACTCACTCT CCAGTGGAAG 
GCATCATGTG ACAATGGCTG TAAAATAGAG AACTATCTTC TGGAATGGGA TGAAGGGAAA 
GGGAGCAAAG AGTTCTGCCA GTGTTATTAT GGACCACAGA AGCAGTTTAG GTTAACAAAG 
TTGTCACCAG CTATGGGATA CACATTCAGA CTTGCAGCAA AAAATGATAT GGGCATGAGT 
GGTTTCAGTG AAGAAGTTTT CTTTTACACC TCAGGCAGTG CTCCTACCAT GCCAGCAAGT 
CCGACACTAA ATAAAGCAGG AATTAGTTGG TTATCCATGC AGTGGACTAA ACCCTCAGGT 
ACACCATCTG ATGAAGGAAT TTCTTATATT TTGGAAATGG AAGATGAAAC TTCGGGATAT 
GGATTTAAGC CAATATACAA TGGTGATGAC CTTACATTTA CAGTGAGAAA TCTGAGAAGA 
AGTACAAAGT ATAAGTTTAG GGTCATTGCA TATAACACTG AAGGCAAGAG CAGCCCAAGT 
GAGGTGGTGG AGTTTGTGAC TTGTCCAGAC AGACCAGGAG CACCCTCAAA GCCTTCTGTC 
AAGGGAAAGG TCCATGCACA TAACTTCAGG ATAGTATGGG ATCCACCTAA GGACAATGGA 
GGAGCAGCAA TCACAAAATA CGTTATGGAG ATGTCTGAAG ACAGCAATGG AAACAAGTGG 
GATGTCGTAT ACAGTGGAAC AGCTCGAGAA TACCTGTGTG ACCATCTGAG TCCAGGCTCT 
TCTTATCGCT TACAAGTCCA TTGCATCAGT GATGGAGGGA AGAGTCAGGT ATCAGATACA 
CTGTTAGTTC AGACTCCAGC GGTGTTCCCT GGGCCATGCC AGCCCCCCAG GCTCCAAGGC 
AGACCAAGAG CAAGAGAAGT GCAAATGAAA TGGGCACCAC CGCAGCTTGA TGGTGGTTCC 
CCAGTCACGT GCTACAGCCT GGAGATGTCG TCTTCTGATG CCAGTGAGCA TAGAGAAGTC 
TACCAAGGAC CTGAACTGGA ATGCACAGTG GCCAGTCTTC TTCCAGGGAA GGCGTACAGC 
TTTCGAGTGC GCGCTGCAAA CAAAACTGGG TTTGGTCCAT TCTCTGAAAA GTGTGAAATG 
ACAACAGCTC CAGGACCACC AGATCAATGT AGACCTCCTC AATTAACATG CAGATCTGCA 
ACCTGTGTCC TTATACACTG GGAGGCCCCC AACAACAATG GAGCTGAGAT TATGGAGTAC 
CGGGTTGAAT GGGGAGGCAT GGAAGGGTGC ATGCAGATCT GCTACTGCGG GCCTGGTTTT 
AATCATGAAG TTAAAGGCCT TTCGCCAGCA ATTGTTTATT ACTGCAGGGT TCAGGCTGTA 
AATATTGCTG GTGCGGGCCA TTTTAGTGAT GTGGTTGCAT GTTTAACGCC GGCATCGGTC 
CCCGCTGCTG TGACAAGTTT GTGGGGAGTA AAAGAAGATG AAGTAAACAG TTCAAATTAC 
TGTCCTTCCA CTTGCCTTGC AATACAGTGG GAAAAACCAT CTGATCATGG TTCAGAGATT 
ACCACTTACA GCATAGACTA TGGGGACAAG CAGCCGATTA CTGTTTACAA AGCAACAAGC 
TACATTCTAG ATAACTTACA GCCAGACACA ACATACAGAA TACGGGTTCA AGCCCTAAAC 
AGCTTGGGAG CTGGTCCTTT CAGCCATTCT GTGAAACTGA AAACCAAGCC ACTACCCCCA 
GAACCACCTC GACTGGAATG TGCGGTGTAC AGTCACCAGA ACCTTAAGCT GAAGTGGGGA 
GACGGTGCAT CCAGGGCATT ACCCACAGAT TCCATTCAGT ATCACCTTCA AATGGAGGAT 
AAGAATGAAA GATTTGTATC CTTATATAGA GGACCATGTC AAACATACAA AGTACAAAGG 
CTCAATGAGT CCACGTCATA CACATTTCGT ATTCAGGCAT GCAATGAGGC TGGTGAGGGG 
CCTTTTTCCT CGGAATATGT TTTTACAACT CCAAAATCAC TTCCAGCTGC CTTGAAAGCT 
CCAAGAATTG ACCGAATTAA TGAACATACC TGTGATGTCA CGTGGGAGTC CTTACAGCCA 
ATGAAAGGAG ATCCGATTAT CTACATTTTG CAATTAATGG TTGGAAAAGA CTCTGATTTT 
AAACAGGTTT ATAAGGGACC TAGTACATCA TTCCGCCACT CTGCCCTCCA GCTAAACTGT 
GAATATCGTT TCCGTGTATG TGCCATTCGC CAGTGCCAAG ATTCTGCAGG ACATCAGGAT 
CTCATTGGTC CATACAGTGC CACCGTCCTC TTCATTTCTC ACCGAAGTGA AACCCAAACC 
AGCACCAACA AAGATACTGT GGAAACCACA AGGACAACAC GGACGCTTAG TGACGAGCAG 
TGCGCTGCTG TCATCCTTGT GCTCTTTGCT ACGTTCTCCA TTCTGATTGC CTTTATCATC 
CAGTACTTTG TTATCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492173 ( XM_002935746.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935792.3 Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X6, mRNA.

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CloneID	OXa52763
Clone ID Related Accession (Same CDS sequence)	XM_002935746.5
Accession Version	XM_002935746.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	fibronectin type-III domain-containing protein 3A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935746.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCC CTGCTCAGGT TCCAATGATG TCACCAAATG GCTCTGTGCC CCCAATATAT 
GTGCCTCCAG GATACGCCCA GCAGGTAATT GAGGATAATG GTGTGCGTAG AGTTGTAGTG 
GTTCCACAAC CCCCTGAGTT CCATACAGGC AGCCATGCGG TCATACATCG ACCCCCACCT 
CCAATACCAG GATTCATTCC TCTTCCAGCT ATGATGCCTC CCCCACCTCG ACACATCTAC 
TCACCAGTGA CTGGAGCAGG AGATGTGGCC TCCCAGTATA CCCCACAGTA TCATGCCGCA 
CAGGTATATG GAGATCTCGA TACTCTTTCT ACGCATGGAC GATCTAACCT CAGAGATGAA 
AGATCCAACA AAATACATGA GAGGTTGCAG AAAAAGCTCA AAGACAGACA TGGAGTACAG 
AAAGAGAAGC TTAACAGCCC CCCATTCTCA CCCAAGAAAA GCACTTCTCC AATAAATGAA 
TCAAATGGAC TTGTAAAAGG CCAGAATTCA GGCTTAAATA CAGGGCAGAC AAAGAACAAG 
CACAAAGGGA AAGGTGGCTC TCCAGGGGAG ACAGAAATAC AAGAAAAAGA TGATGAAACA 
AAGGCACTCG AAGCACTGCT CTCAACGGTT TCCCAACCTG TGGTATCCGA TATCCAGGCC 
AGGTCTGTAA CATTATCATG GTCATCACCT TCCAGCCTTA TCAGTGAGCA GAATGATGGG 
ACATGTAGCC CTGAACCATA CGCTTATGAA ATCCTGCTGT CGAGCAGTGG GAAAGATGGA 
AAGTACAAGG TTGCTTATAG TGGAGAAAAG GCCACTGTTA CTATGGATGA CTTGAGACCT 
GCAACTGATT ATCATGCACG AGTTCAAGCG GAGTCGAACT GTGTAAAGGG AAACTTGTCA 
GAAGCGGTCA GTTTCACTAC ACTTTGCTGT GAACCAGACA CACCAAATAA TCCACGAATA 
ACTAATCGGA CCAAAAATTC ACTCACTCTC CAGTGGAAGG CATCATGTGA CAATGGCTGT 
AAAATAGAGA ACTATCTTCT GGAATGGGAT GAAGGGAAAG GGAGCAAAGA GTTCTGCCAG 
TGTTATTATG GACCACAGAA GCAGTTTAGG TTAACAAAGT TGTCACCAGC TATGGGATAC 
ACATTCAGAC TTGCAGCAAA AAATGATATG GGCATGAGTG GTTTCAGTGA AGAAGTTTTC 
TTTTACACCT CAGGCAGTGC TCCTACCATG CCAGCAAGTC CGACACTAAA TAAAGCAGGA 
ATTAGTTGGT TATCCATGCA GTGGACTAAA CCCTCAGGTA CACCATCTGA TGAAGGAATT 
TCTTATATTT TGGAAATGGA AGATGAAACT TCGGGATATG GATTTAAGCC AATATACAAT 
GGTGATGACC TTACATTTAC AGTGAGAAAT CTGAGAAGAA GTACAAAGTA TAAGTTTAGG 
GTCATTGCAT ATAACACTGA AGGCAAGAGC AGCCCAAGTG AGGTGGTGGA GTTTGTGACT 
TGTCCAGACA GACCAGGAGC ACCCTCAAAG CCTTCTGTCA AGGGAAAGGT CCATGCACAT 
AACTTCAGGA TAGTATGGGA TCCACCTAAG GACAATGGAG GAGCAGCAAT CACAAAATAC 
GTTATGGAGA TGTCTGAAGA CAGCAATGGA AACAAGTGGG ATGTCGTATA CAGTGGAACA 
GCTCGAGAAT ACCTGTGTGA CCATCTGAGT CCAGGCTCTT CTTATCGCTT ACAAGTCCAT 
TGCATCAGTG ATGGAGGGAA GAGTCAGGTA TCAGATACAC TGTTAGTTCA GACTCCAGCG 
GTGTTCCCTG GGCCATGCCA GCCCCCCAGG CTCCAAGGCA GACCAAGAGC AAGAGAAGTG 
CAAATGAAAT GGGCACCACC GCAGCTTGAT GGTGGTTCCC CAGTCACGTG CTACAGCCTG 
GAGATGTCGT CTTCTGATGC CAGTGAGCAT AGAGAAGTCT ACCAAGGACC TGAACTGGAA 
TGCACAGTGG CCAGTCTTCT TCCAGGGAAG GCGTACAGCT TTCGAGTGCG CGCTGCAAAC 
AAAACTGGGT TTGGTCCATT CTCTGAAAAG TGTGAAATGA CAACAGCTCC AGGACCACCA 
GATCAATGTA GACCTCCTCA ATTAACATGC AGATCTGCAA CCTGTGTCCT TATACACTGG 
GAGGCCCCCA ACAACAATGG AGCTGAGATT ATGGAGTACC GGGTTGAATG GGGAGGCATG 
GAAGGGTGCA TGCAGATCTG CTACTGCGGG CCTGGTTTTA ATCATGAAGT TAAAGGCCTT 
TCGCCAGCAA TTGTTTATTA CTGCAGGGTT CAGGCTGTAA ATATTGCTGG TGCGGGCCAT 
TTTAGTGATG TGGTTGCATG TTTAACGCCG GCATCGGTCC CCGCTGCTGT GACAAGTTTG 
TGGGGAGTAA AAGAAGATGA AGTAAACAGT TCAAATTACT GTCCTTCCAC TTGCCTTGCA 
ATACAGTGGG AAAAACCATC TGATCATGGT TCAGAGATTA CCACTTACAG CATAGACTAT 
GGGGACAAGC AGCCGATTAC TGTTTACAAA GCAACAAGCT ACATTCTAGA TAACTTACAG 
CCAGACACAA CATACAGAAT ACGGGTTCAA GCCCTAAACA GCTTGGGAGC TGGTCCTTTC 
AGCCATTCTG TGAAACTGAA AACCAAGCCA CTACCCCCAG AACCACCTCG ACTGGAATGT 
GCGGTGTACA GTCACCAGAA CCTTAAGCTG AAGTGGGGAG ACGGTGCATC CAGGGCATTA 
CCCACAGATT CCATTCAGTA TCACCTTCAA ATGGAGGATA AGAATGAAAG ATTTGTATCC 
TTATATAGAG GACCATGTCA AACATACAAA GTACAAAGGC TCAATGAGTC CACGTCATAC 
ACATTTCGTA TTCAGGCATG CAATGAGGCT GGTGAGGGGC CTTTTTCCTC GGAATATGTT 
TTTACAACTC CAAAATCACT TCCAGCTGCC TTGAAAGCTC CAAGAATTGA CCGAATTAAT 
GAACATACCT GTGATGTCAC GTGGGAGTCC TTACAGCCAA TGAAAGGAGA TCCGATTATC 
TACATTTTGC AATTAATGGT TGGAAAAGAC TCTGATTTTA AACAGGTTTA TAAGGGACCT 
AGTACATCAT TCCGCCACTC TGCCCTCCAG CTAAACTGTG AATATCGTTT CCGTGTATGT 
GCCATTCGCC AGTGCCAAGA TTCTGCAGGA CATCAGGATC TCATTGGTCC ATACAGTGCC 
ACCGTCCTCT TCATTTCTCA CCGAAGTGAA ACCCAAACCA GCACCAACAA AGATACTGTG 
GAAACCACAA GGACAACACG GACGCTTAGT GACGAGCAGT GCGCTGCTGT CATCCTTGTG 
CTCTTTGCTA CGTTCTCCAT TCTGATTGCC TTTATCATCC AGTACTTTGT TATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935792.3
CDS	77..3496
Translation	MSGPAQVPMMSPNGSVPPIYVPPGYAQQVIEDNGVRRVVVVPQPPEFHTGSHAVIHRPPPPIPGFIPLPAMMPPPPRHIYSPVTGAGDVASQYTPQYHAAQVYGDLDTLSTHGRSNLRDERSNKIHERLQKKLKDRHGVQKEKLNSPPFSPKKSTSPINESNGLVKGQNSGLNTGQTKNKHKGKGGSPGETEIQEKDDETKALEALLSTVSQPVVSDIQARSVTLSWSSPSSLISEQNDGTCSPEPYAYEILLSSSGKDGKYKVAYSGEKATVTMDDLRPATDYHARVQAESNCVKGNLSEAVSFTTLCCEPDTPNNPRITNRTKNSLTLQWKASCDNGCKIENYLLEWDEGKGSKEFCQCYYGPQKQFRLTKLSPAMGYTFRLAAKNDMGMSGFSEEVFFYTSGSAPTMPASPTLNKAGISWLSMQWTKPSGTPSDEGISYILEMEDETSGYGFKPIYNGDDLTFTVRNLRRSTKYKFRVIAYNTEGKSSPSEVVEFVTCPDRPGAPSKPSVKGKVHAHNFRIVWDPPKDNGGAAITKYVMEMSEDSNGNKWDVVYSGTAREYLCDHLSPGSSYRLQVHCISDGGKSQVSDTLLVQTPAVFPGPCQPPRLQGRPRAREVQMKWAPPQLDGGSPVTCYSLEMSSSDASEHREVYQGPELECTVASLLPGKAYSFRVRAANKTGFGPFSEKCEMTTAPGPPDQCRPPQLTCRSATCVLIHWEAPNNNGAEIMEYRVEWGGMEGCMQICYCGPGFNHEVKGLSPAIVYYCRVQAVNIAGAGHFSDVVACLTPASVPAAVTSLWGVKEDEVNSSNYCPSTCLAIQWEKPSDHGSEITTYSIDYGDKQPITVYKATSYILDNLQPDTTYRIRVQALNSLGAGPFSHSVKLKTKPLPPEPPRLECAVYSHQNLKLKWGDGASRALPTDSIQYHLQMEDKNERFVSLYRGPCQTYKVQRLNESTSYTFRIQACNEAGEGPFSSEYVFTTPKSLPAALKAPRIDRINEHTCDVTWESLQPMKGDPIIYILQLMVGKDSDFKQVYKGPSTSFRHSALQLNCEYRFRVCAIRQCQDSAGHQDLIGPYSATVLFISHRSETQTSTNKDTVETTRTTRTLSDEQCAAVILVLFATFSILIAFIIQYFVIK

Target ORF information:

RefSeq Version	XM_002935746.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis fibronectin type III domain containing 3A (LOC100492173), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935746.5

ORF Insert Sequence:

ATGAGTGGCC CTGCTCAGGT TCCAATGATG TCACCAAATG GCTCTGTGCC CCCAATATAT 
GTGCCTCCAG GATACGCCCA GCAGGTAATT GAGGATAATG GTGTGCGTAG AGTTGTAGTG 
GTTCCACAAC CCCCTGAGTT CCATACAGGC AGCCATGCGG TCATACATCG ACCCCCACCT 
CCAATACCAG GATTCATTCC TCTTCCAGCT ATGATGCCTC CCCCACCTCG ACACATCTAC 
TCACCAGTGA CTGGAGCAGG AGATGTGGCC TCCCAGTATA CCCCACAGTA TCATGCCGCA 
CAGGTATATG GAGATCTCGA TACTCTTTCT ACGCATGGAC GATCTAACCT CAGAGATGAA 
AGATCCAACA AAATACATGA GAGGTTGCAG AAAAAGCTCA AAGACAGACA TGGAGTACAG 
AAAGAGAAGC TTAACAGCCC CCCATTCTCA CCCAAGAAAA GCACTTCTCC AATAAATGAA 
TCAAATGGAC TTGTAAAAGG CCAGAATTCA GGCTTAAATA CAGGGCAGAC AAAGAACAAG 
CACAAAGGGA AAGGTGGCTC TCCAGGGGAG ACAGAAATAC AAGAAAAAGA TGATGAAACA 
AAGGCACTCG AAGCACTGCT CTCAACGGTT TCCCAACCTG TGGTATCCGA TATCCAGGCC 
AGGTCTGTAA CATTATCATG GTCATCACCT TCCAGCCTTA TCAGTGAGCA GAATGATGGG 
ACATGTAGCC CTGAACCATA CGCTTATGAA ATCCTGCTGT CGAGCAGTGG GAAAGATGGA 
AAGTACAAGG TTGCTTATAG TGGAGAAAAG GCCACTGTTA CTATGGATGA CTTGAGACCT 
GCAACTGATT ATCATGCACG AGTTCAAGCG GAGTCGAACT GTGTAAAGGG AAACTTGTCA 
GAAGCGGTCA GTTTCACTAC ACTTTGCTGT GAACCAGACA CACCAAATAA TCCACGAATA 
ACTAATCGGA CCAAAAATTC ACTCACTCTC CAGTGGAAGG CATCATGTGA CAATGGCTGT 
AAAATAGAGA ACTATCTTCT GGAATGGGAT GAAGGGAAAG GGAGCAAAGA GTTCTGCCAG 
TGTTATTATG GACCACAGAA GCAGTTTAGG TTAACAAAGT TGTCACCAGC TATGGGATAC 
ACATTCAGAC TTGCAGCAAA AAATGATATG GGCATGAGTG GTTTCAGTGA AGAAGTTTTC 
TTTTACACCT CAGGCAGTGC TCCTACCATG CCAGCAAGTC CGACACTAAA TAAAGCAGGA 
ATTAGTTGGT TATCCATGCA GTGGACTAAA CCCTCAGGTA CACCATCTGA TGAAGGAATT 
TCTTATATTT TGGAAATGGA AGATGAAACT TCGGGATATG GATTTAAGCC AATATACAAT 
GGTGATGACC TTACATTTAC AGTGAGAAAT CTGAGAAGAA GTACAAAGTA TAAGTTTAGG 
GTCATTGCAT ATAACACTGA AGGCAAGAGC AGCCCAAGTG AGGTGGTGGA GTTTGTGACT 
TGTCCAGACA GACCAGGAGC ACCCTCAAAG CCTTCTGTCA AGGGAAAGGT CCATGCACAT 
AACTTCAGGA TAGTATGGGA TCCACCTAAG GACAATGGAG GAGCAGCAAT CACAAAATAC 
GTTATGGAGA TGTCTGAAGA CAGCAATGGA AACAAGTGGG ATGTCGTATA CAGTGGAACA 
GCTCGAGAAT ACCTGTGTGA CCATCTGAGT CCAGGCTCTT CTTATCGCTT ACAAGTCCAT 
TGCATCAGTG ATGGAGGGAA GAGTCAGGTA TCAGATACAC TGTTAGTTCA GACTCCAGCG 
GTGTTCCCTG GGCCATGCCA GCCCCCCAGG CTCCAAGGCA GACCAAGAGC AAGAGAAGTG 
CAAATGAAAT GGGCACCACC GCAGCTTGAT GGTGGTTCCC CAGTCACGTG CTACAGCCTG 
GAGATGTCGT CTTCTGATGC CAGTGAGCAT AGAGAAGTCT ACCAAGGACC TGAACTGGAA 
TGCACAGTGG CCAGTCTTCT TCCAGGGAAG GCGTACAGCT TTCGAGTGCG CGCTGCAAAC 
AAAACTGGGT TTGGTCCATT CTCTGAAAAG TGTGAAATGA CAACAGCTCC AGGACCACCA 
GATCAATGTA GACCTCCTCA ATTAACATGC AGATCTGCAA CCTGTGTCCT TATACACTGG 
GAGGCCCCCA ACAACAATGG AGCTGAGATT ATGGAGTACC GGGTTGAATG GGGAGGCATG 
GAAGGGTGCA TGCAGATCTG CTACTGCGGG CCTGGTTTTA ATCATGAAGT TAAAGGCCTT 
TCGCCAGCAA TTGTTTATTA CTGCAGGGTT CAGGCTGTAA ATATTGCTGG TGCGGGCCAT 
TTTAGTGATG TGGTTGCATG TTTAACGCCG GCATCGGTCC CCGCTGCTGT GACAAGTTTG 
TGGGGAGTAA AAGAAGATGA AGTAAACAGT TCAAATTACT GTCCTTCCAC TTGCCTTGCA 
ATACAGTGGG AAAAACCATC TGATCATGGT TCAGAGATTA CCACTTACAG CATAGACTAT 
GGGGACAAGC AGCCGATTAC TGTTTACAAA GCAACAAGCT ACATTCTAGA TAACTTACAG 
CCAGACACAA CATACAGAAT ACGGGTTCAA GCCCTAAACA GCTTGGGAGC TGGTCCTTTC 
AGCCATTCTG TGAAACTGAA AACCAAGCCA CTACCCCCAG AACCACCTCG ACTGGAATGT 
GCGGTGTACA GTCACCAGAA CCTTAAGCTG AAGTGGGGAG ACGGTGCATC CAGGGCATTA 
CCCACAGATT CCATTCAGTA TCACCTTCAA ATGGAGGATA AGAATGAAAG ATTTGTATCC 
TTATATAGAG GACCATGTCA AACATACAAA GTACAAAGGC TCAATGAGTC CACGTCATAC 
ACATTTCGTA TTCAGGCATG CAATGAGGCT GGTGAGGGGC CTTTTTCCTC GGAATATGTT 
TTTACAACTC CAAAATCACT TCCAGCTGCC TTGAAAGCTC CAAGAATTGA CCGAATTAAT 
GAACATACCT GTGATGTCAC GTGGGAGTCC TTACAGCCAA TGAAAGGAGA TCCGATTATC 
TACATTTTGC AATTAATGGT TGGAAAAGAC TCTGATTTTA AACAGGTTTA TAAGGGACCT 
AGTACATCAT TCCGCCACTC TGCCCTCCAG CTAAACTGTG AATATCGTTT CCGTGTATGT 
GCCATTCGCC AGTGCCAAGA TTCTGCAGGA CATCAGGATC TCATTGGTCC ATACAGTGCC 
ACCGTCCTCT TCATTTCTCA CCGAAGTGAA ACCCAAACCA GCACCAACAA AGATACTGTG 
GAAACCACAA GGACAACACG GACGCTTAGT GACGAGCAGT GCGCTGCTGT CATCCTTGTG 
CTCTTTGCTA CGTTCTCCAT TCTGATTGCC TTTATCATCC AGTACTTTGT TATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492173 ( XM_004911963.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912020.1 Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X1, mRNA.

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CloneID	OXa06143
Clone ID Related Accession (Same CDS sequence)	XM_004911963.3 , XM_018091725.1 , XM_004911964.3 , XM_018091724.1
Accession Version	XM_004911963.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	fibronectin type-III domain-containing protein 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911963.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGAAAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912020.1
CDS	733..4341
Translation	MYVLSVMADHPPPPLDSTPLLTTEIPLLPAPIVNGEGGQQVILVQVNPGEAFTIRREDGQFQCITGPAQVPMMSPNGSVPPIYVPPGYAQQVIEDNGVRRVVVVPQPPEFHTGSHAVIHRPPPPIPGFIPLPAMMPPPPRHIYSPVTGAGDVASQYTPQYHAAQVYGDLDTLSTHGRSNLRDERSNKIHERLQKKLKDRHGVQKEKLNSPPFSPKKSTSPINESNGLVKGQNSGLNTGQTKNKHKGKGGSPGETEIQEKDDETKALEALLSTVSQPVVSDIQARSVTLSWSSPSSLISEQNDGTCSPEPYAYEILLSSSGKDGKYKVAYSGEKATVTMDDLRPATDYHARVQAESNCVKGNLSEAVSFTTLCCEPETPNNPRITNRTKNSLTLQWKASCDNGCKIENYLLEWDEGKGSKEFCQCYYGPQKQFRLTKLSPAMGYTFRLAAKNDMGMSGFSEEVFFYTSGSAPTMPASPTLNKAGISWLSMQWTKPSGTPSDEGISYILEMEDETSGYGFKPIYNGDDLTFTVRNLRRSTKYKFRVIAYNTEGKSSPSEVVEFVTCPDRPGAPSKPSVKGKVHAHNFRIVWDPPKDNGGAAITKYVMEMSEDSNGNKWDVVYSGTAREYLCDHLSPGSSYRLQVHCISDGGKSQVSDTLLVQTPAVFPGPCQPPRLQGRPRAREVQMKWAPPQLDGGSPVTCYSLEMSSSDASEHREVYQGPELECTVASLLPGKAYSFRVRAANKTGFGPFSEKCEMTTAPGPPDQCRPPQLTCRSATCVLIHWEAPNNNGAEIMEYRVEWGGMEGCMQICYCGPGFNHEVKGLSPAIVYYCRVQAVNIAGAGHFSDVVACLTPASVPAAVTSLWGVKEDEVNSSNYCPSTCLAIQWEKPSDHGSEITTYSIDYGDKQPITVYKATSYILDNLQPDTTYRIRVQALNSLGAGPFSHSVKLKTKPLPPEPPRLECAVYSHQNLKLKWGDGASRALPTDSIQYHLQMEDKNERFVSLYRGPCQTYKVQRLNESTSYTFRIQACNEAGEGPFSSEYVFTTPKSLPAALKAPRIDRINEHTCDVTWESLQPMKGDPIIYILQLMVGKDSDFKQVYKGPSTSFRHSALQLNCEYRFRVCAIRQCQDSAGHQDLIGPYSATVLFISHRSETQTSTNKDTVETTRTTRTLSDEQCAAVILVLFATFSILIAFIIQYFVIK

Target ORF information:

RefSeq Version	XM_004911963.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911963.3

ORF Insert Sequence:

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGAAAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492173 ( XM_018091725.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947214.1 Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X4, mRNA.

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CloneID	OXa06143
Clone ID Related Accession (Same CDS sequence)	XM_004911963.3 , XM_018091725.1 , XM_004911964.3 , XM_018091724.1
Accession Version	XM_018091725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	fibronectin type-III domain-containing protein 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGAAAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947214.1
CDS	131..3739
Translation	MYVLSVMADHPPPPLDSTPLLTTEIPLLPAPIVNGEGGQQVILVQVNPGEAFTIRREDGQFQCITGPAQVPMMSPNGSVPPIYVPPGYAQQVIEDNGVRRVVVVPQPPEFHTGSHAVIHRPPPPIPGFIPLPAMMPPPPRHIYSPVTGAGDVASQYTPQYHAAQVYGDLDTLSTHGRSNLRDERSNKIHERLQKKLKDRHGVQKEKLNSPPFSPKKSTSPINESNGLVKGQNSGLNTGQTKNKHKGKGGSPGETEIQEKDDETKALEALLSTVSQPVVSDIQARSVTLSWSSPSSLISEQNDGTCSPEPYAYEILLSSSGKDGKYKVAYSGEKATVTMDDLRPATDYHARVQAESNCVKGNLSEAVSFTTLCCEPETPNNPRITNRTKNSLTLQWKASCDNGCKIENYLLEWDEGKGSKEFCQCYYGPQKQFRLTKLSPAMGYTFRLAAKNDMGMSGFSEEVFFYTSGSAPTMPASPTLNKAGISWLSMQWTKPSGTPSDEGISYILEMEDETSGYGFKPIYNGDDLTFTVRNLRRSTKYKFRVIAYNTEGKSSPSEVVEFVTCPDRPGAPSKPSVKGKVHAHNFRIVWDPPKDNGGAAITKYVMEMSEDSNGNKWDVVYSGTAREYLCDHLSPGSSYRLQVHCISDGGKSQVSDTLLVQTPAVFPGPCQPPRLQGRPRAREVQMKWAPPQLDGGSPVTCYSLEMSSSDASEHREVYQGPELECTVASLLPGKAYSFRVRAANKTGFGPFSEKCEMTTAPGPPDQCRPPQLTCRSATCVLIHWEAPNNNGAEIMEYRVEWGGMEGCMQICYCGPGFNHEVKGLSPAIVYYCRVQAVNIAGAGHFSDVVACLTPASVPAAVTSLWGVKEDEVNSSNYCPSTCLAIQWEKPSDHGSEITTYSIDYGDKQPITVYKATSYILDNLQPDTTYRIRVQALNSLGAGPFSHSVKLKTKPLPPEPPRLECAVYSHQNLKLKWGDGASRALPTDSIQYHLQMEDKNERFVSLYRGPCQTYKVQRLNESTSYTFRIQACNEAGEGPFSSEYVFTTPKSLPAALKAPRIDRINEHTCDVTWESLQPMKGDPIIYILQLMVGKDSDFKQVYKGPSTSFRHSALQLNCEYRFRVCAIRQCQDSAGHQDLIGPYSATVLFISHRSETQTSTNKDTVETTRTTRTLSDEQCAAVILVLFATFSILIAFIIQYFVIK

Target ORF information:

RefSeq Version	XM_018091725.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091725.1

ORF Insert Sequence:

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGAAAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492173 ( XM_004911964.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004912021.1 Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X2, mRNA.

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CloneID	OXa06143
Clone ID Related Accession (Same CDS sequence)	XM_004911963.3 , XM_018091725.1 , XM_004911964.3 , XM_018091724.1
Accession Version	XM_004911964.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	fibronectin type-III domain-containing protein 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911964.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGAAAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004912021.1
CDS	641..4249
Translation	MYVLSVMADHPPPPLDSTPLLTTEIPLLPAPIVNGEGGQQVILVQVNPGEAFTIRREDGQFQCITGPAQVPMMSPNGSVPPIYVPPGYAQQVIEDNGVRRVVVVPQPPEFHTGSHAVIHRPPPPIPGFIPLPAMMPPPPRHIYSPVTGAGDVASQYTPQYHAAQVYGDLDTLSTHGRSNLRDERSNKIHERLQKKLKDRHGVQKEKLNSPPFSPKKSTSPINESNGLVKGQNSGLNTGQTKNKHKGKGGSPGETEIQEKDDETKALEALLSTVSQPVVSDIQARSVTLSWSSPSSLISEQNDGTCSPEPYAYEILLSSSGKDGKYKVAYSGEKATVTMDDLRPATDYHARVQAESNCVKGNLSEAVSFTTLCCEPETPNNPRITNRTKNSLTLQWKASCDNGCKIENYLLEWDEGKGSKEFCQCYYGPQKQFRLTKLSPAMGYTFRLAAKNDMGMSGFSEEVFFYTSGSAPTMPASPTLNKAGISWLSMQWTKPSGTPSDEGISYILEMEDETSGYGFKPIYNGDDLTFTVRNLRRSTKYKFRVIAYNTEGKSSPSEVVEFVTCPDRPGAPSKPSVKGKVHAHNFRIVWDPPKDNGGAAITKYVMEMSEDSNGNKWDVVYSGTAREYLCDHLSPGSSYRLQVHCISDGGKSQVSDTLLVQTPAVFPGPCQPPRLQGRPRAREVQMKWAPPQLDGGSPVTCYSLEMSSSDASEHREVYQGPELECTVASLLPGKAYSFRVRAANKTGFGPFSEKCEMTTAPGPPDQCRPPQLTCRSATCVLIHWEAPNNNGAEIMEYRVEWGGMEGCMQICYCGPGFNHEVKGLSPAIVYYCRVQAVNIAGAGHFSDVVACLTPASVPAAVTSLWGVKEDEVNSSNYCPSTCLAIQWEKPSDHGSEITTYSIDYGDKQPITVYKATSYILDNLQPDTTYRIRVQALNSLGAGPFSHSVKLKTKPLPPEPPRLECAVYSHQNLKLKWGDGASRALPTDSIQYHLQMEDKNERFVSLYRGPCQTYKVQRLNESTSYTFRIQACNEAGEGPFSSEYVFTTPKSLPAALKAPRIDRINEHTCDVTWESLQPMKGDPIIYILQLMVGKDSDFKQVYKGPSTSFRHSALQLNCEYRFRVCAIRQCQDSAGHQDLIGPYSATVLFISHRSETQTSTNKDTVETTRTTRTLSDEQCAAVILVLFATFSILIAFIIQYFVIK

Target ORF information:

RefSeq Version	XM_004911964.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911964.3

ORF Insert Sequence:

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGAAAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492173 ( XM_018091724.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947213.1 Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa06143
Clone ID Related Accession (Same CDS sequence)	XM_004911963.3 , XM_018091725.1 , XM_004911964.3 , XM_018091724.1
Accession Version	XM_018091724.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	fibronectin type-III domain-containing protein 3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGAAAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947213.1
CDS	181..3789
Translation	MYVLSVMADHPPPPLDSTPLLTTEIPLLPAPIVNGEGGQQVILVQVNPGEAFTIRREDGQFQCITGPAQVPMMSPNGSVPPIYVPPGYAQQVIEDNGVRRVVVVPQPPEFHTGSHAVIHRPPPPIPGFIPLPAMMPPPPRHIYSPVTGAGDVASQYTPQYHAAQVYGDLDTLSTHGRSNLRDERSNKIHERLQKKLKDRHGVQKEKLNSPPFSPKKSTSPINESNGLVKGQNSGLNTGQTKNKHKGKGGSPGETEIQEKDDETKALEALLSTVSQPVVSDIQARSVTLSWSSPSSLISEQNDGTCSPEPYAYEILLSSSGKDGKYKVAYSGEKATVTMDDLRPATDYHARVQAESNCVKGNLSEAVSFTTLCCEPETPNNPRITNRTKNSLTLQWKASCDNGCKIENYLLEWDEGKGSKEFCQCYYGPQKQFRLTKLSPAMGYTFRLAAKNDMGMSGFSEEVFFYTSGSAPTMPASPTLNKAGISWLSMQWTKPSGTPSDEGISYILEMEDETSGYGFKPIYNGDDLTFTVRNLRRSTKYKFRVIAYNTEGKSSPSEVVEFVTCPDRPGAPSKPSVKGKVHAHNFRIVWDPPKDNGGAAITKYVMEMSEDSNGNKWDVVYSGTAREYLCDHLSPGSSYRLQVHCISDGGKSQVSDTLLVQTPAVFPGPCQPPRLQGRPRAREVQMKWAPPQLDGGSPVTCYSLEMSSSDASEHREVYQGPELECTVASLLPGKAYSFRVRAANKTGFGPFSEKCEMTTAPGPPDQCRPPQLTCRSATCVLIHWEAPNNNGAEIMEYRVEWGGMEGCMQICYCGPGFNHEVKGLSPAIVYYCRVQAVNIAGAGHFSDVVACLTPASVPAAVTSLWGVKEDEVNSSNYCPSTCLAIQWEKPSDHGSEITTYSIDYGDKQPITVYKATSYILDNLQPDTTYRIRVQALNSLGAGPFSHSVKLKTKPLPPEPPRLECAVYSHQNLKLKWGDGASRALPTDSIQYHLQMEDKNERFVSLYRGPCQTYKVQRLNESTSYTFRIQACNEAGEGPFSSEYVFTTPKSLPAALKAPRIDRINEHTCDVTWESLQPMKGDPIIYILQLMVGKDSDFKQVYKGPSTSFRHSALQLNCEYRFRVCAIRQCQDSAGHQDLIGPYSATVLFISHRSETQTSTNKDTVETTRTTRTLSDEQCAAVILVLFATFSILIAFIIQYFVIK

Target ORF information:

RefSeq Version	XM_018091724.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091724.1

ORF Insert Sequence:

ATGTATGTCT TATCCGTAAT GGCAGATCAT CCGCCTCCTC CACTGGACAG CACACCGCTC 
CTAACCACAG AGATACCTCT ACTCCCTGCA CCCATTGTTA ATGGGGAAGG AGGCCAACAG 
GTTATTCTGG TGCAAGTTAA TCCAGGGGAG GCATTTACAA TAAGAAGAGA AGATGGGCAG 
TTTCAGTGCA TCACAGGCCC TGCTCAGGTT CCAATGATGT CACCAAATGG CTCTGTGCCC 
CCAATATATG TGCCTCCAGG ATACGCCCAG CAGGTAATTG AGGATAATGG TGTGCGTAGA 
GTTGTAGTGG TTCCACAACC CCCTGAGTTC CATACAGGCA GCCATGCGGT CATACATCGA 
CCCCCACCTC CAATACCAGG ATTCATTCCT CTTCCAGCTA TGATGCCTCC CCCACCTCGA 
CACATCTACT CACCAGTGAC TGGAGCAGGA GATGTGGCCT CCCAGTATAC CCCACAGTAT 
CATGCCGCAC AGGTATATGG AGATCTCGAT ACTCTTTCTA CGCATGGACG ATCTAACCTC 
AGAGATGAAA GATCCAACAA AATACATGAG AGGTTGCAGA AAAAGCTCAA AGACAGACAT 
GGAGTACAGA AAGAGAAGCT TAACAGCCCC CCATTCTCAC CCAAGAAAAG CACTTCTCCA 
ATAAATGAAT CAAATGGACT TGTAAAAGGC CAGAATTCAG GCTTAAATAC AGGGCAGACA 
AAGAACAAGC ACAAAGGGAA AGGTGGCTCT CCAGGGGAGA CAGAAATACA AGAAAAAGAT 
GATGAAACAA AGGCACTCGA AGCACTGCTC TCAACGGTTT CCCAACCTGT GGTATCCGAT 
ATCCAGGCCA GGTCTGTAAC ATTATCATGG TCATCACCTT CCAGCCTTAT CAGTGAGCAG 
AATGATGGGA CATGTAGCCC TGAACCATAC GCTTATGAAA TCCTGCTGTC GAGCAGTGGG 
AAAGATGGAA AGTACAAGGT TGCTTATAGT GGAGAAAAGG CCACTGTTAC TATGGATGAC 
TTGAGACCTG CAACTGATTA TCATGCACGA GTTCAAGCGG AGTCGAACTG TGTAAAGGGA 
AACTTGTCAG AAGCGGTCAG TTTCACTACA CTTTGCTGTG AACCAGAAAC ACCAAATAAT 
CCACGAATAA CTAATCGGAC CAAAAATTCA CTCACTCTCC AGTGGAAGGC ATCATGTGAC 
AATGGCTGTA AAATAGAGAA CTATCTTCTG GAATGGGATG AAGGGAAAGG GAGCAAAGAG 
TTCTGCCAGT GTTATTATGG ACCACAGAAG CAGTTTAGGT TAACAAAGTT GTCACCAGCT 
ATGGGATACA CATTCAGACT TGCAGCAAAA AATGATATGG GCATGAGTGG TTTCAGTGAA 
GAAGTTTTCT TTTACACCTC AGGCAGTGCT CCTACCATGC CAGCAAGTCC GACACTAAAT 
AAAGCAGGAA TTAGTTGGTT ATCCATGCAG TGGACTAAAC CCTCAGGTAC ACCATCTGAT 
GAAGGAATTT CTTATATTTT GGAAATGGAA GATGAAACTT CGGGATATGG ATTTAAGCCA 
ATATACAATG GTGATGACCT TACATTTACA GTGAGAAATC TGAGAAGAAG TACAAAGTAT 
AAGTTTAGGG TCATTGCATA TAACACTGAA GGCAAGAGCA GCCCAAGTGA GGTGGTGGAG 
TTTGTGACTT GTCCAGACAG ACCAGGAGCA CCCTCAAAGC CTTCTGTCAA GGGAAAGGTC 
CATGCACATA ACTTCAGGAT AGTATGGGAT CCACCTAAGG ACAATGGAGG AGCAGCAATC 
ACAAAATACG TTATGGAGAT GTCTGAAGAC AGCAATGGAA ACAAGTGGGA TGTCGTATAC 
AGTGGAACAG CTCGAGAATA CCTGTGTGAC CATCTGAGTC CAGGCTCTTC TTATCGCTTA 
CAAGTCCATT GCATCAGTGA TGGAGGGAAG AGTCAGGTAT CAGATACACT GTTAGTTCAG 
ACTCCAGCGG TGTTCCCTGG GCCATGCCAG CCCCCCAGGC TCCAAGGCAG ACCAAGAGCA 
AGAGAAGTGC AAATGAAATG GGCACCACCG CAGCTTGATG GTGGTTCCCC AGTCACGTGC 
TACAGCCTGG AGATGTCGTC TTCTGATGCC AGTGAGCATA GAGAAGTCTA CCAAGGACCT 
GAACTGGAAT GCACAGTGGC CAGTCTTCTT CCAGGGAAGG CGTACAGCTT TCGAGTGCGC 
GCTGCAAACA AAACTGGGTT TGGTCCATTC TCTGAAAAGT GTGAAATGAC AACAGCTCCA 
GGACCACCAG ATCAATGTAG ACCTCCTCAA TTAACATGCA GATCTGCAAC CTGTGTCCTT 
ATACACTGGG AGGCCCCCAA CAACAATGGA GCTGAGATTA TGGAGTACCG GGTTGAATGG 
GGAGGCATGG AAGGGTGCAT GCAGATCTGC TACTGCGGGC CTGGTTTTAA TCATGAAGTT 
AAAGGCCTTT CGCCAGCAAT TGTTTATTAC TGCAGGGTTC AGGCTGTAAA TATTGCTGGT 
GCGGGCCATT TTAGTGATGT GGTTGCATGT TTAACGCCGG CATCGGTCCC CGCTGCTGTG 
ACAAGTTTGT GGGGAGTAAA AGAAGATGAA GTAAACAGTT CAAATTACTG TCCTTCCACT 
TGCCTTGCAA TACAGTGGGA AAAACCATCT GATCATGGTT CAGAGATTAC CACTTACAGC 
ATAGACTATG GGGACAAGCA GCCGATTACT GTTTACAAAG CAACAAGCTA CATTCTAGAT 
AACTTACAGC CAGACACAAC ATACAGAATA CGGGTTCAAG CCCTAAACAG CTTGGGAGCT 
GGTCCTTTCA GCCATTCTGT GAAACTGAAA ACCAAGCCAC TACCCCCAGA ACCACCTCGA 
CTGGAATGTG CGGTGTACAG TCACCAGAAC CTTAAGCTGA AGTGGGGAGA CGGTGCATCC 
AGGGCATTAC CCACAGATTC CATTCAGTAT CACCTTCAAA TGGAGGATAA GAATGAAAGA 
TTTGTATCCT TATATAGAGG ACCATGTCAA ACATACAAAG TACAAAGGCT CAATGAGTCC 
ACGTCATACA CATTTCGTAT TCAGGCATGC AATGAGGCTG GTGAGGGGCC TTTTTCCTCG 
GAATATGTTT TTACAACTCC AAAATCACTT CCAGCTGCCT TGAAAGCTCC AAGAATTGAC 
CGAATTAATG AACATACCTG TGATGTCACG TGGGAGTCCT TACAGCCAAT GAAAGGAGAT 
CCGATTATCT ACATTTTGCA ATTAATGGTT GGAAAAGACT CTGATTTTAA ACAGGTTTAT 
AAGGGACCTA GTACATCATT CCGCCACTCT GCCCTCCAGC TAAACTGTGA ATATCGTTTC 
CGTGTATGTG CCATTCGCCA GTGCCAAGAT TCTGCAGGAC ATCAGGATCT CATTGGTCCA 
TACAGTGCCA CCGTCCTCTT CATTTCTCAC CGAAGTGAAA CCCAAACCAG CACCAACAAA 
GATACTGTGG AAACCACAAG GACAACACGG ACGCTTAGTG ACGAGCAGTG CGCTGCTGTC 
ATCCTTGTGC TCTTTGCTAC GTTCTCCATT CTGATTGCCT TTATCATCCA GTACTTTGTT 
ATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100492173 ( XM_002935746.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935792.2 Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X5, mRNA.

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CloneID	OXa06144
Clone ID Related Accession (Same CDS sequence)	XM_002935746.4
Accession Version	XM_002935746.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	fibronectin type-III domain-containing protein 3A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935746.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCC CTGCTCAGGT TCCAATGATG TCACCAAATG GCTCTGTGCC CCCAATATAT 
GTGCCTCCAG GATACGCCCA GCAGGTAATT GAGGATAATG GTGTGCGTAG AGTTGTAGTG 
GTTCCACAAC CCCCTGAGTT CCATACAGGC AGCCATGCGG TCATACATCG ACCCCCACCT 
CCAATACCAG GATTCATTCC TCTTCCAGCT ATGATGCCTC CCCCACCTCG ACACATCTAC 
TCACCAGTGA CTGGAGCAGG AGATGTGGCC TCCCAGTATA CCCCACAGTA TCATGCCGCA 
CAGGTATATG GAGATCTCGA TACTCTTTCT ACGCATGGAC GATCTAACCT CAGAGATGAA 
AGATCCAACA AAATACATGA GAGGTTGCAG AAAAAGCTCA AAGACAGACA TGGAGTACAG 
AAAGAGAAGC TTAACAGCCC CCCATTCTCA CCCAAGAAAA GCACTTCTCC AATAAATGAA 
TCAAATGGAC TTGTAAAAGG CCAGAATTCA GGCTTAAATA CAGGGCAGAC AAAGAACAAG 
CACAAAGGGA AAGGTGGCTC TCCAGGGGAG ACAGAAATAC AAGAAAAAGA TGATGAAACA 
AAGGCACTCG AAGCACTGCT CTCAACGGTT TCCCAACCTG TGGTATCCGA TATCCAGGCC 
AGGTCTGTAA CATTATCATG GTCATCACCT TCCAGCCTTA TCAGTGAGCA GAATGATGGG 
ACATGTAGCC CTGAACCATA CGCTTATGAA ATCCTGCTGT CGAGCAGTGG GAAAGATGGA 
AAGTACAAGG TTGCTTATAG TGGAGAAAAG GCCACTGTTA CTATGGATGA CTTGAGACCT 
GCAACTGATT ATCATGCACG AGTTCAAGCG GAGTCGAACT GTGTAAAGGG AAACTTGTCA 
GAAGCGGTCA GTTTCACTAC ACTTTGCTGT GAACCAGAAA CACCAAATAA TCCACGAATA 
ACTAATCGGA CCAAAAATTC ACTCACTCTC CAGTGGAAGG CATCATGTGA CAATGGCTGT 
AAAATAGAGA ACTATCTTCT GGAATGGGAT GAAGGGAAAG GGAGCAAAGA GTTCTGCCAG 
TGTTATTATG GACCACAGAA GCAGTTTAGG TTAACAAAGT TGTCACCAGC TATGGGATAC 
ACATTCAGAC TTGCAGCAAA AAATGATATG GGCATGAGTG GTTTCAGTGA AGAAGTTTTC 
TTTTACACCT CAGGCAGTGC TCCTACCATG CCAGCAAGTC CGACACTAAA TAAAGCAGGA 
ATTAGTTGGT TATCCATGCA GTGGACTAAA CCCTCAGGTA CACCATCTGA TGAAGGAATT 
TCTTATATTT TGGAAATGGA AGATGAAACT TCGGGATATG GATTTAAGCC AATATACAAT 
GGTGATGACC TTACATTTAC AGTGAGAAAT CTGAGAAGAA GTACAAAGTA TAAGTTTAGG 
GTCATTGCAT ATAACACTGA AGGCAAGAGC AGCCCAAGTG AGGTGGTGGA GTTTGTGACT 
TGTCCAGACA GACCAGGAGC ACCCTCAAAG CCTTCTGTCA AGGGAAAGGT CCATGCACAT 
AACTTCAGGA TAGTATGGGA TCCACCTAAG GACAATGGAG GAGCAGCAAT CACAAAATAC 
GTTATGGAGA TGTCTGAAGA CAGCAATGGA AACAAGTGGG ATGTCGTATA CAGTGGAACA 
GCTCGAGAAT ACCTGTGTGA CCATCTGAGT CCAGGCTCTT CTTATCGCTT ACAAGTCCAT 
TGCATCAGTG ATGGAGGGAA GAGTCAGGTA TCAGATACAC TGTTAGTTCA GACTCCAGCG 
GTGTTCCCTG GGCCATGCCA GCCCCCCAGG CTCCAAGGCA GACCAAGAGC AAGAGAAGTG 
CAAATGAAAT GGGCACCACC GCAGCTTGAT GGTGGTTCCC CAGTCACGTG CTACAGCCTG 
GAGATGTCGT CTTCTGATGC CAGTGAGCAT AGAGAAGTCT ACCAAGGACC TGAACTGGAA 
TGCACAGTGG CCAGTCTTCT TCCAGGGAAG GCGTACAGCT TTCGAGTGCG CGCTGCAAAC 
AAAACTGGGT TTGGTCCATT CTCTGAAAAG TGTGAAATGA CAACAGCTCC AGGACCACCA 
GATCAATGTA GACCTCCTCA ATTAACATGC AGATCTGCAA CCTGTGTCCT TATACACTGG 
GAGGCCCCCA ACAACAATGG AGCTGAGATT ATGGAGTACC GGGTTGAATG GGGAGGCATG 
GAAGGGTGCA TGCAGATCTG CTACTGCGGG CCTGGTTTTA ATCATGAAGT TAAAGGCCTT 
TCGCCAGCAA TTGTTTATTA CTGCAGGGTT CAGGCTGTAA ATATTGCTGG TGCGGGCCAT 
TTTAGTGATG TGGTTGCATG TTTAACGCCG GCATCGGTCC CCGCTGCTGT GACAAGTTTG 
TGGGGAGTAA AAGAAGATGA AGTAAACAGT TCAAATTACT GTCCTTCCAC TTGCCTTGCA 
ATACAGTGGG AAAAACCATC TGATCATGGT TCAGAGATTA CCACTTACAG CATAGACTAT 
GGGGACAAGC AGCCGATTAC TGTTTACAAA GCAACAAGCT ACATTCTAGA TAACTTACAG 
CCAGACACAA CATACAGAAT ACGGGTTCAA GCCCTAAACA GCTTGGGAGC TGGTCCTTTC 
AGCCATTCTG TGAAACTGAA AACCAAGCCA CTACCCCCAG AACCACCTCG ACTGGAATGT 
GCGGTGTACA GTCACCAGAA CCTTAAGCTG AAGTGGGGAG ACGGTGCATC CAGGGCATTA 
CCCACAGATT CCATTCAGTA TCACCTTCAA ATGGAGGATA AGAATGAAAG ATTTGTATCC 
TTATATAGAG GACCATGTCA AACATACAAA GTACAAAGGC TCAATGAGTC CACGTCATAC 
ACATTTCGTA TTCAGGCATG CAATGAGGCT GGTGAGGGGC CTTTTTCCTC GGAATATGTT 
TTTACAACTC CAAAATCACT TCCAGCTGCC TTGAAAGCTC CAAGAATTGA CCGAATTAAT 
GAACATACCT GTGATGTCAC GTGGGAGTCC TTACAGCCAA TGAAAGGAGA TCCGATTATC 
TACATTTTGC AATTAATGGT TGGAAAAGAC TCTGATTTTA AACAGGTTTA TAAGGGACCT 
AGTACATCAT TCCGCCACTC TGCCCTCCAG CTAAACTGTG AATATCGTTT CCGTGTATGT 
GCCATTCGCC AGTGCCAAGA TTCTGCAGGA CATCAGGATC TCATTGGTCC ATACAGTGCC 
ACCGTCCTCT TCATTTCTCA CCGAAGTGAA ACCCAAACCA GCACCAACAA AGATACTGTG 
GAAACCACAA GGACAACACG GACGCTTAGT GACGAGCAGT GCGCTGCTGT CATCCTTGTG 
CTCTTTGCTA CGTTCTCCAT TCTGATTGCC TTTATCATCC AGTACTTTGT TATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935792.2
CDS	79..3498
Translation	MSGPAQVPMMSPNGSVPPIYVPPGYAQQVIEDNGVRRVVVVPQPPEFHTGSHAVIHRPPPPIPGFIPLPAMMPPPPRHIYSPVTGAGDVASQYTPQYHAAQVYGDLDTLSTHGRSNLRDERSNKIHERLQKKLKDRHGVQKEKLNSPPFSPKKSTSPINESNGLVKGQNSGLNTGQTKNKHKGKGGSPGETEIQEKDDETKALEALLSTVSQPVVSDIQARSVTLSWSSPSSLISEQNDGTCSPEPYAYEILLSSSGKDGKYKVAYSGEKATVTMDDLRPATDYHARVQAESNCVKGNLSEAVSFTTLCCEPETPNNPRITNRTKNSLTLQWKASCDNGCKIENYLLEWDEGKGSKEFCQCYYGPQKQFRLTKLSPAMGYTFRLAAKNDMGMSGFSEEVFFYTSGSAPTMPASPTLNKAGISWLSMQWTKPSGTPSDEGISYILEMEDETSGYGFKPIYNGDDLTFTVRNLRRSTKYKFRVIAYNTEGKSSPSEVVEFVTCPDRPGAPSKPSVKGKVHAHNFRIVWDPPKDNGGAAITKYVMEMSEDSNGNKWDVVYSGTAREYLCDHLSPGSSYRLQVHCISDGGKSQVSDTLLVQTPAVFPGPCQPPRLQGRPRAREVQMKWAPPQLDGGSPVTCYSLEMSSSDASEHREVYQGPELECTVASLLPGKAYSFRVRAANKTGFGPFSEKCEMTTAPGPPDQCRPPQLTCRSATCVLIHWEAPNNNGAEIMEYRVEWGGMEGCMQICYCGPGFNHEVKGLSPAIVYYCRVQAVNIAGAGHFSDVVACLTPASVPAAVTSLWGVKEDEVNSSNYCPSTCLAIQWEKPSDHGSEITTYSIDYGDKQPITVYKATSYILDNLQPDTTYRIRVQALNSLGAGPFSHSVKLKTKPLPPEPPRLECAVYSHQNLKLKWGDGASRALPTDSIQYHLQMEDKNERFVSLYRGPCQTYKVQRLNESTSYTFRIQACNEAGEGPFSSEYVFTTPKSLPAALKAPRIDRINEHTCDVTWESLQPMKGDPIIYILQLMVGKDSDFKQVYKGPSTSFRHSALQLNCEYRFRVCAIRQCQDSAGHQDLIGPYSATVLFISHRSETQTSTNKDTVETTRTTRTLSDEQCAAVILVLFATFSILIAFIIQYFVIK

Target ORF information:

RefSeq Version	XM_002935746.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis fibronectin type III domain containing 3A (fndc3a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935746.4

ORF Insert Sequence:

ATGAGTGGCC CTGCTCAGGT TCCAATGATG TCACCAAATG GCTCTGTGCC CCCAATATAT 
GTGCCTCCAG GATACGCCCA GCAGGTAATT GAGGATAATG GTGTGCGTAG AGTTGTAGTG 
GTTCCACAAC CCCCTGAGTT CCATACAGGC AGCCATGCGG TCATACATCG ACCCCCACCT 
CCAATACCAG GATTCATTCC TCTTCCAGCT ATGATGCCTC CCCCACCTCG ACACATCTAC 
TCACCAGTGA CTGGAGCAGG AGATGTGGCC TCCCAGTATA CCCCACAGTA TCATGCCGCA 
CAGGTATATG GAGATCTCGA TACTCTTTCT ACGCATGGAC GATCTAACCT CAGAGATGAA 
AGATCCAACA AAATACATGA GAGGTTGCAG AAAAAGCTCA AAGACAGACA TGGAGTACAG 
AAAGAGAAGC TTAACAGCCC CCCATTCTCA CCCAAGAAAA GCACTTCTCC AATAAATGAA 
TCAAATGGAC TTGTAAAAGG CCAGAATTCA GGCTTAAATA CAGGGCAGAC AAAGAACAAG 
CACAAAGGGA AAGGTGGCTC TCCAGGGGAG ACAGAAATAC AAGAAAAAGA TGATGAAACA 
AAGGCACTCG AAGCACTGCT CTCAACGGTT TCCCAACCTG TGGTATCCGA TATCCAGGCC 
AGGTCTGTAA CATTATCATG GTCATCACCT TCCAGCCTTA TCAGTGAGCA GAATGATGGG 
ACATGTAGCC CTGAACCATA CGCTTATGAA ATCCTGCTGT CGAGCAGTGG GAAAGATGGA 
AAGTACAAGG TTGCTTATAG TGGAGAAAAG GCCACTGTTA CTATGGATGA CTTGAGACCT 
GCAACTGATT ATCATGCACG AGTTCAAGCG GAGTCGAACT GTGTAAAGGG AAACTTGTCA 
GAAGCGGTCA GTTTCACTAC ACTTTGCTGT GAACCAGAAA CACCAAATAA TCCACGAATA 
ACTAATCGGA CCAAAAATTC ACTCACTCTC CAGTGGAAGG CATCATGTGA CAATGGCTGT 
AAAATAGAGA ACTATCTTCT GGAATGGGAT GAAGGGAAAG GGAGCAAAGA GTTCTGCCAG 
TGTTATTATG GACCACAGAA GCAGTTTAGG TTAACAAAGT TGTCACCAGC TATGGGATAC 
ACATTCAGAC TTGCAGCAAA AAATGATATG GGCATGAGTG GTTTCAGTGA AGAAGTTTTC 
TTTTACACCT CAGGCAGTGC TCCTACCATG CCAGCAAGTC CGACACTAAA TAAAGCAGGA 
ATTAGTTGGT TATCCATGCA GTGGACTAAA CCCTCAGGTA CACCATCTGA TGAAGGAATT 
TCTTATATTT TGGAAATGGA AGATGAAACT TCGGGATATG GATTTAAGCC AATATACAAT 
GGTGATGACC TTACATTTAC AGTGAGAAAT CTGAGAAGAA GTACAAAGTA TAAGTTTAGG 
GTCATTGCAT ATAACACTGA AGGCAAGAGC AGCCCAAGTG AGGTGGTGGA GTTTGTGACT 
TGTCCAGACA GACCAGGAGC ACCCTCAAAG CCTTCTGTCA AGGGAAAGGT CCATGCACAT 
AACTTCAGGA TAGTATGGGA TCCACCTAAG GACAATGGAG GAGCAGCAAT CACAAAATAC 
GTTATGGAGA TGTCTGAAGA CAGCAATGGA AACAAGTGGG ATGTCGTATA CAGTGGAACA 
GCTCGAGAAT ACCTGTGTGA CCATCTGAGT CCAGGCTCTT CTTATCGCTT ACAAGTCCAT 
TGCATCAGTG ATGGAGGGAA GAGTCAGGTA TCAGATACAC TGTTAGTTCA GACTCCAGCG 
GTGTTCCCTG GGCCATGCCA GCCCCCCAGG CTCCAAGGCA GACCAAGAGC AAGAGAAGTG 
CAAATGAAAT GGGCACCACC GCAGCTTGAT GGTGGTTCCC CAGTCACGTG CTACAGCCTG 
GAGATGTCGT CTTCTGATGC CAGTGAGCAT AGAGAAGTCT ACCAAGGACC TGAACTGGAA 
TGCACAGTGG CCAGTCTTCT TCCAGGGAAG GCGTACAGCT TTCGAGTGCG CGCTGCAAAC 
AAAACTGGGT TTGGTCCATT CTCTGAAAAG TGTGAAATGA CAACAGCTCC AGGACCACCA 
GATCAATGTA GACCTCCTCA ATTAACATGC AGATCTGCAA CCTGTGTCCT TATACACTGG 
GAGGCCCCCA ACAACAATGG AGCTGAGATT ATGGAGTACC GGGTTGAATG GGGAGGCATG 
GAAGGGTGCA TGCAGATCTG CTACTGCGGG CCTGGTTTTA ATCATGAAGT TAAAGGCCTT 
TCGCCAGCAA TTGTTTATTA CTGCAGGGTT CAGGCTGTAA ATATTGCTGG TGCGGGCCAT 
TTTAGTGATG TGGTTGCATG TTTAACGCCG GCATCGGTCC CCGCTGCTGT GACAAGTTTG 
TGGGGAGTAA AAGAAGATGA AGTAAACAGT TCAAATTACT GTCCTTCCAC TTGCCTTGCA 
ATACAGTGGG AAAAACCATC TGATCATGGT TCAGAGATTA CCACTTACAG CATAGACTAT 
GGGGACAAGC AGCCGATTAC TGTTTACAAA GCAACAAGCT ACATTCTAGA TAACTTACAG 
CCAGACACAA CATACAGAAT ACGGGTTCAA GCCCTAAACA GCTTGGGAGC TGGTCCTTTC 
AGCCATTCTG TGAAACTGAA AACCAAGCCA CTACCCCCAG AACCACCTCG ACTGGAATGT 
GCGGTGTACA GTCACCAGAA CCTTAAGCTG AAGTGGGGAG ACGGTGCATC CAGGGCATTA 
CCCACAGATT CCATTCAGTA TCACCTTCAA ATGGAGGATA AGAATGAAAG ATTTGTATCC 
TTATATAGAG GACCATGTCA AACATACAAA GTACAAAGGC TCAATGAGTC CACGTCATAC 
ACATTTCGTA TTCAGGCATG CAATGAGGCT GGTGAGGGGC CTTTTTCCTC GGAATATGTT 
TTTACAACTC CAAAATCACT TCCAGCTGCC TTGAAAGCTC CAAGAATTGA CCGAATTAAT 
GAACATACCT GTGATGTCAC GTGGGAGTCC TTACAGCCAA TGAAAGGAGA TCCGATTATC 
TACATTTTGC AATTAATGGT TGGAAAAGAC TCTGATTTTA AACAGGTTTA TAAGGGACCT 
AGTACATCAT TCCGCCACTC TGCCCTCCAG CTAAACTGTG AATATCGTTT CCGTGTATGT 
GCCATTCGCC AGTGCCAAGA TTCTGCAGGA CATCAGGATC TCATTGGTCC ATACAGTGCC 
ACCGTCCTCT TCATTTCTCA CCGAAGTGAA ACCCAAACCA GCACCAACAA AGATACTGTG 
GAAACCACAA GGACAACACG GACGCTTAGT GACGAGCAGT GCGCTGCTGT CATCCTTGTG 
CTCTTTGCTA CGTTCTCCAT TCTGATTGCC TTTATCATCC AGTACTTTGT TATCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pan troglodytes (chimpanzee)	FNDC3A	XP_522753.2
Canis lupus familiaris (dog)	FNDC3A	XP_005633930.1
Xenopus tropicalis (tropical clawed frog)	LOC100492173	XP_004912021.1
Homo sapiens (human)	FNDC3A	NP_001265367.1
	LOC705570	XP_002808191.1
Bos taurus (cattle)	FNDC3A	NP_001192575.1
Rattus norvegicus (Norway rat)	Fndc3a	NP_001100748.1
Mus musculus (house mouse)	Fndc3a	NP_997519.2
Gallus gallus (chicken)	FNDC3A	NP_001012844.1
Drosophila melanogaster (fruit fly)	CG42389	NP_723981.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009522	XP_310164.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC100492173 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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