kcnma1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following kcnma1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kcnma1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa12981 XM_004916014.4
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa12984 XM_031906047.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa35192 XM_012966937.3
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa35194 XM_012966939.3
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa35195 XM_012966940.3
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa35197 XM_012966942.3
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa35200 XM_012966946.3
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa45786 XM_018095895.1
Latest version!
Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa45787 XM_018095896.1
Latest version!
Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa62475 XM_031906033.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa62476 XM_031906034.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa62477 XM_031906035.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62478 XM_031906036.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62479 XM_031906037.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62480 XM_031906038.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62481 XM_031906039.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62482 XM_031906040.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62483 XM_031906041.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62484 XM_031906042.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62485 XM_031906043.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62486 XM_031906044.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62487 XM_031906045.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62488 XM_031906046.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62489 XM_031906048.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa62490 XM_031906049.1
Latest version!
Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa35188 XM_012966933.2
Latest version!
Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa35193 XM_012966938.2
Latest version!
Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OXa12981 XM_004916014.3 Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa35197 XM_012966942.2 Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa35200 XM_012966946.2 Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa35195 XM_012966940.2 Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa35192 XM_012966937.2 Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa35194 XM_012966939.2 Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa12981
    Clone ID Related Accession (Same CDS sequence) XM_004916014.4 , XM_004916014.3
    Accession Version XM_004916014.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916014.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCT ACAAGGGAAG AAAATAGATT AAGTATCCAC
    AGGCTTTCTA TTCATCCCCA GACAGCCAAA GCTAGCTATT CTGTGGCCAG TTCCAAACTC
    TGCACAGAAC AGCAGGAGCC TGTGCCTCTT GTTAATAACC GCAAAGGAAG CCTGTTCTTG
    CCATGTGATT CCTCTCTCCT GCACCTGCAG TTACTGGGTT CCTCTGGCAC TGAACACCGT
    ACCAGTATTA AATTGCAGCG TGCACTGAGC TTGCCAGGCA AATACCGCTA CCACCCCAAT
    CAGCCAGTAC TCATCCAAAA GCAGTTTGAA GATGAGCAAC CATCAGCCTT GTCACCAAAA
    AAAAAGCAAC GAAATGGAGG AATGAGGCAA TCTCCAAACT CTTCACCTAA CATGATGAGG
    CATGATCCCC TTCTTATGAC TGGAAATGAT CAAATTGATA ATATGGATTC TAGCAGTGTT
    AAAAGATATG ATTCTACTGG TATGTTCCAC TGGTGTCCAG CCAAGGAATT GGATAAAGTG
    CTTCTGACAC GGAGTGAAGC TGCCATGACA GTCCTCAGTG GGCATGTGGT TGTCTGCATT
    TTTGGAGACA TGACATCGGC ACTGATTGGA GTTCGGAATT TGGTGATGCC ACTGAGAGCC
    AGCAATTTTC ATTACCATGA GTTGAAACAT ATAGTCTTTG TTGGGTCCCT TGATTACCTT
    AAAAGAGAAT GGGAAACACT ACACAACTTC CCAAAGGTTT CCATCTTGCC TGGCACACCA
    TTAAGTCGAG CAGATCTGAG AGCTGTCAAC ATTAACCTGT GTGACATGTG CGTTATTCTG
    TCAGCCAATC AGAATAATAT TGATGATACA TCACTGCAGG ACAAAGAATG CATTTTGGCA
    TCTCTCAACA TCAAATCCAT GCAGTTTGAT GACAGCATAG GGCTCTTGCA AGCTAACTCT
    CAAGGGTTTA CGCCCCCAGG AATGGAGAGA TCCTCACCTG ATAATAGCCC AGTGCATGGT
    GTTGCAAGAC AGGCATCCAT AACTACAGGG GCCAACATTC CCATAATTAC AGAACTTGCT
    AAGCCTGGCA AACTGCTGCC TTTGATTTCA TTCAGTCAGG AAAAAAACAG TGGGACACAC
    ATACCAATTA TTACAGAGTT GGTGAACGAC TCAAATGTTC AGTTCTTGGA CCAGGATGAT
    GATGATGACC CAGATACAGA GTTATACTTG ACTCAGCCCT TCGCCTGTGG GACCGCCTTT
    GCTGTCAGTG TGTTAGACTC CCTTATGAGT GCAACATACT TCAATGATAA CATTCTGACT
    CTAATCAGAA CTCTGGTGAC TGGGGGAGCA ACACCAGAGC TGGAAGCCCT CATTGCAGAA
    GAGAACGCTC TGCGTGGGGG TTATAGCACC CCACAAACTC TAGCAAACAG AGACCGCTGT
    CGAGTAGCCC AATTAGCCTT GTATGATGGA CCATTTGCAG ATTTGGGGGA TGGTGGCTGT
    TATGGAGACC TTTACTGCAA AGCATTAAAA ACTTACAACA TGCTGTGCTT TGGTATATAC
    CGACTCCGAG ATGCCCACCT CAGCACACCC AGCCAGTGTA CCAAAAGGTA CGTTATCACC
    AACCCTCCAT ATGAGTTTGA ACTGGTTCCC ACAGATCTCA TCTTTTGCCT GATGCAGTTC
    GACCATAATG CCAGCCAGTC CCGAGCTAGC CTGTCTCACT CTTCACACTC CTCGCACTCA
    TCTAGCAAAA AAAGTTCATC TGTTACCTCC ATTCTACACA CAGCCTCAGC CAACCGTCAG
    AACAGAGTCA AGGCTCGAGA TTCCCGTGAC AAACAAAAGA CGAATAGAAT GGGCCAAGCA
    GAAAAGAAAT GGTATACAGA TGAAACGGAA AACACTTATC CCAGAAACAT TCAGATTAAG
    CCAATGAGCA CACATATGGC AAATCAGATT AATCAATACA AATCAACAAG CAGCTTGATA
    CCGCCAATAC GAGAGGTCGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916071.1
    CDS1061..5053
    Translation

    Target ORF information:

    RefSeq Version XM_004916014.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916014.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCT ACAAGGGAAG AAAATAGATT AAGTATCCAC
    AGGCTTTCTA TTCATCCCCA GACAGCCAAA GCTAGCTATT CTGTGGCCAG TTCCAAACTC
    TGCACAGAAC AGCAGGAGCC TGTGCCTCTT GTTAATAACC GCAAAGGAAG CCTGTTCTTG
    CCATGTGATT CCTCTCTCCT GCACCTGCAG TTACTGGGTT CCTCTGGCAC TGAACACCGT
    ACCAGTATTA AATTGCAGCG TGCACTGAGC TTGCCAGGCA AATACCGCTA CCACCCCAAT
    CAGCCAGTAC TCATCCAAAA GCAGTTTGAA GATGAGCAAC CATCAGCCTT GTCACCAAAA
    AAAAAGCAAC GAAATGGAGG AATGAGGCAA TCTCCAAACT CTTCACCTAA CATGATGAGG
    CATGATCCCC TTCTTATGAC TGGAAATGAT CAAATTGATA ATATGGATTC TAGCAGTGTT
    AAAAGATATG ATTCTACTGG TATGTTCCAC TGGTGTCCAG CCAAGGAATT GGATAAAGTG
    CTTCTGACAC GGAGTGAAGC TGCCATGACA GTCCTCAGTG GGCATGTGGT TGTCTGCATT
    TTTGGAGACA TGACATCGGC ACTGATTGGA GTTCGGAATT TGGTGATGCC ACTGAGAGCC
    AGCAATTTTC ATTACCATGA GTTGAAACAT ATAGTCTTTG TTGGGTCCCT TGATTACCTT
    AAAAGAGAAT GGGAAACACT ACACAACTTC CCAAAGGTTT CCATCTTGCC TGGCACACCA
    TTAAGTCGAG CAGATCTGAG AGCTGTCAAC ATTAACCTGT GTGACATGTG CGTTATTCTG
    TCAGCCAATC AGAATAATAT TGATGATACA TCACTGCAGG ACAAAGAATG CATTTTGGCA
    TCTCTCAACA TCAAATCCAT GCAGTTTGAT GACAGCATAG GGCTCTTGCA AGCTAACTCT
    CAAGGGTTTA CGCCCCCAGG AATGGAGAGA TCCTCACCTG ATAATAGCCC AGTGCATGGT
    GTTGCAAGAC AGGCATCCAT AACTACAGGG GCCAACATTC CCATAATTAC AGAACTTGCT
    AAGCCTGGCA AACTGCTGCC TTTGATTTCA TTCAGTCAGG AAAAAAACAG TGGGACACAC
    ATACCAATTA TTACAGAGTT GGTGAACGAC TCAAATGTTC AGTTCTTGGA CCAGGATGAT
    GATGATGACC CAGATACAGA GTTATACTTG ACTCAGCCCT TCGCCTGTGG GACCGCCTTT
    GCTGTCAGTG TGTTAGACTC CCTTATGAGT GCAACATACT TCAATGATAA CATTCTGACT
    CTAATCAGAA CTCTGGTGAC TGGGGGAGCA ACACCAGAGC TGGAAGCCCT CATTGCAGAA
    GAGAACGCTC TGCGTGGGGG TTATAGCACC CCACAAACTC TAGCAAACAG AGACCGCTGT
    CGAGTAGCCC AATTAGCCTT GTATGATGGA CCATTTGCAG ATTTGGGGGA TGGTGGCTGT
    TATGGAGACC TTTACTGCAA AGCATTAAAA ACTTACAACA TGCTGTGCTT TGGTATATAC
    CGACTCCGAG ATGCCCACCT CAGCACACCC AGCCAGTGTA CCAAAAGGTA CGTTATCACC
    AACCCTCCAT ATGAGTTTGA ACTGGTTCCC ACAGATCTCA TCTTTTGCCT GATGCAGTTC
    GACCATAATG CCAGCCAGTC CCGAGCTAGC CTGTCTCACT CTTCACACTC CTCGCACTCA
    TCTAGCAAAA AAAGTTCATC TGTTACCTCC ATTCTACACA CAGCCTCAGC CAACCGTCAG
    AACAGAGTCA AGGCTCGAGA TTCCCGTGAC AAACAAAAGA CGAATAGAAT GGGCCAAGCA
    GAAAAGAAAT GGTATACAGA TGAAACGGAA AACACTTATC CCAGAAACAT TCAGATTAAG
    CCAATGAGCA CACATATGGC AAATCAGATT AATCAATACA AATCAACAAG CAGCTTGATA
    CCGCCAATAC GAGAGGTCGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12984
    Clone ID Related Accession (Same CDS sequence) XM_031906047.1
    Accession Version XM_031906047.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT ATTTATTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG CTAAGCCTGG CAAACTGCTG CCTTTGATTT CATTCAGTCA GGAAAAAAAC
    AGTGGGACAC ACATACCAAT TATTACAGAG TTGGTGAACG ACTCAAATGT TCAGTTCTTG
    GACCAGGATG ATGATGATGA CCCAGATACA GAGTTATACT TGACTCAGCC CTTCGCCTGT
    GGGACCGCCT TTGCTGTCAG TGTGTTAGAC TCCCTTATGA GTGCAACATA CTTCAATGAT
    AACATTCTGA CTCTAATCAG AACTCTGGTG ACTGGGGGAG CAACACCAGA GCTGGAAGCC
    CTCATTGCAG AAGAGAACGC TCTGCGTGGG GGTTATAGCA CCCCACAAAC TCTAGCAAAC
    AGAGACCGCT GTCGAGTAGC CCAATTAGCC TTGTATGATG GACCATTTGC AGATTTGGGG
    GATGGTGGCT GTTATGGAGA CCTTTACTGC AAAGCATTAA AAACTTACAA CATGCTGTGC
    TTTGGTATAT ACCGACTCCG AGATGCCCAC CTCAGCACAC CCAGCCAGTG TACCAAAAGG
    TACGTTATCA CCAACCCTCC ATATGAGTTT GAACTGGTTC CCACAGATCT CATCTTTTGC
    CTGATGCAGT TCGACCATAA TGCCAGCCAG TCCCGAGCTA GCCTGTCTCA CTCTTCACAC
    TCCTCGCACT CATCTAGCAA AAAAAGTTCA TCTGTTACCT CCATTCTACA CACAGCCTCA
    GCCAACCGTC AGAACAGAGT CAAGGCTCGA GATTCCCGTG ACAAACAAAA GTATGTTCTG
    GATGACGCAA TGCTGGGGAG AGGGGTGAGG AGGACAGGGG GAGCGATGTC TATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761907.1
    CDS1052..4648
    Translation

    Target ORF information:

    RefSeq Version XM_031906047.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT ATTTATTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG CTAAGCCTGG CAAACTGCTG CCTTTGATTT CATTCAGTCA GGAAAAAAAC
    AGTGGGACAC ACATACCAAT TATTACAGAG TTGGTGAACG ACTCAAATGT TCAGTTCTTG
    GACCAGGATG ATGATGATGA CCCAGATACA GAGTTATACT TGACTCAGCC CTTCGCCTGT
    GGGACCGCCT TTGCTGTCAG TGTGTTAGAC TCCCTTATGA GTGCAACATA CTTCAATGAT
    AACATTCTGA CTCTAATCAG AACTCTGGTG ACTGGGGGAG CAACACCAGA GCTGGAAGCC
    CTCATTGCAG AAGAGAACGC TCTGCGTGGG GGTTATAGCA CCCCACAAAC TCTAGCAAAC
    AGAGACCGCT GTCGAGTAGC CCAATTAGCC TTGTATGATG GACCATTTGC AGATTTGGGG
    GATGGTGGCT GTTATGGAGA CCTTTACTGC AAAGCATTAA AAACTTACAA CATGCTGTGC
    TTTGGTATAT ACCGACTCCG AGATGCCCAC CTCAGCACAC CCAGCCAGTG TACCAAAAGG
    TACGTTATCA CCAACCCTCC ATATGAGTTT GAACTGGTTC CCACAGATCT CATCTTTTGC
    CTGATGCAGT TCGACCATAA TGCCAGCCAG TCCCGAGCTA GCCTGTCTCA CTCTTCACAC
    TCCTCGCACT CATCTAGCAA AAAAAGTTCA TCTGTTACCT CCATTCTACA CACAGCCTCA
    GCCAACCGTC AGAACAGAGT CAAGGCTCGA GATTCCCGTG ACAAACAAAA GTATGTTCTG
    GATGACGCAA TGCTGGGGAG AGGGGTGAGG AGGACAGGGG GAGCGATGTC TATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35192
    Clone ID Related Accession (Same CDS sequence) XM_012966937.2 , XM_012966937.3
    Accession Version XM_012966937.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCC AAGATGTCCA CCCGCAAGAG GCTGATACAG
    GCATGTTGTA TGGGGTGCTC TGAGATAGAC TGCTCTTGCA TGTCAGGCAC TTTGCGTAGT
    AACATGGGAA CCCTTGAACA AGCCTTCCCA ATTTCTCCTG TCTCTGTTAA TGATTTCTCC
    ACCAGTTTAC GTGGCTTTGA AGATGAGCAA CCATCAGCCT TGTCACCAAA AAAAAAGCAA
    CGAAATGGAG GAATGAGGCA ATCTCCAAAC TCTTCACCTA ACATGATGAG GCATGATCCC
    CTTCTTATGA CTGGAAATGA TCAAATTGAT AATATGGATT CTAGCAGTGT TAAAAGATAT
    GATTCTACTG GTATGTTCCA CTGGTGTCCA GCCAAGGAAT TGGATAAAGT GCTTCTGACA
    CGGAGTGAAG CTGCCATGAC AGTCCTCAGT GGGCATGTGG TTGTCTGCAT TTTTGGAGAC
    ATGACATCGG CACTGATTGG AGTTCGGAAT TTGGTGATGC CACTGAGAGC CAGCAATTTT
    CATTACCATG AGTTGAAACA TATAGTCTTT GTTGGGTCCC TTGATTACCT TAAAAGAGAA
    TGGGAAACAC TACACAACTT CCCAAAGGTT TCCATCTTGC CTGGCACACC ATTAAGTCGA
    GCAGATCTGA GAGCTGTCAA CATTAACCTG TGTGACATGT GCGTTATTCT GTCAGCCAAT
    CAGAATAATA TTGATGATAC ATCACTGCAG GACAAAGAAT GCATTTTGGC ATCTCTCAAC
    ATCAAATCCA TGCAGTTTGA TGACAGCATA GGGCTCTTGC AAGCTAACTC TCAAGGGTTT
    ACGCCCCCAG GAATGGAGAG ATCCTCACCT GATAATAGCC CAGTGCATGG TGTTGCAAGA
    CAGGCATCCA TAACTACAGG GGCCAACATT CCCATAATTA CAGAACTTGT GAACGACTCA
    AATGTTCAGT TCTTGGACCA GGATGATGAT GATGACCCAG ATACAGAGTT ATACTTGACT
    CAGCCCTTCG CCTGTGGGAC CGCCTTTGCT GTCAGTGTGT TAGACTCCCT TATGAGTGCA
    ACATACTTCA ATGATAACAT TCTGACTCTA ATCAGAACTC TGGTGACTGG GGGAGCAACA
    CCAGAGCTGG AAGCCCTCAT TGCAGAAGAG AACGCTCTGC GTGGGGGTTA TAGCACCCCA
    CAAACTCTAG CAAACAGAGA CCGCTGTCGA GTAGCCCAAT TAGCCTTGTA TGATGGACCA
    TTTGCAGATT TGGGGGATGG TGGCTGTTAT GGAGACCTTT ACTGCAAAGC ATTAAAAACT
    TACAACATGC TGTGCTTTGG TATATACCGA CTCCGAGATG CCCACCTCAG CACACCCAGC
    CAGTGTACCA AAAGGTACGT TATCACCAAC CCTCCATATG AGTTTGAACT GGTTCCCACA
    GATCTCATCT TTTGCCTGAT GCAGTTCGAC CATAATGCCA GCCAGTCCCG AGCTAGCCTG
    TCTCACTCTT CACACTCCTC GCACTCATCT AGCAAAAAAA GTTCATCTGT TACCTCCATT
    CTACACACAG CCTCAGCCAA CCGTCAGAAC AGAGTCAAGG CTCGAGATTC CCGTGACAAA
    CAAAAGACGA ATAGAATGGG CCAAGCAGAA AAGAAATGGT ATACAGATGA AACGGAAAAC
    ACTTATCCCA GAAACATTCA GATTAAGCCA ATGAGCACAC ATATGGCAAA TCAGATTAAT
    CAATACAAAT CAACAAGCAG CTTGATACCG CCAATACGAG AGGTCGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822391.1
    CDS1051..4830
    Translation

    Target ORF information:

    RefSeq Version XM_012966937.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966937.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCC AAGATGTCCA CCCGCAAGAG GCTGATACAG
    GCATGTTGTA TGGGGTGCTC TGAGATAGAC TGCTCTTGCA TGTCAGGCAC TTTGCGTAGT
    AACATGGGAA CCCTTGAACA AGCCTTCCCA ATTTCTCCTG TCTCTGTTAA TGATTTCTCC
    ACCAGTTTAC GTGGCTTTGA AGATGAGCAA CCATCAGCCT TGTCACCAAA AAAAAAGCAA
    CGAAATGGAG GAATGAGGCA ATCTCCAAAC TCTTCACCTA ACATGATGAG GCATGATCCC
    CTTCTTATGA CTGGAAATGA TCAAATTGAT AATATGGATT CTAGCAGTGT TAAAAGATAT
    GATTCTACTG GTATGTTCCA CTGGTGTCCA GCCAAGGAAT TGGATAAAGT GCTTCTGACA
    CGGAGTGAAG CTGCCATGAC AGTCCTCAGT GGGCATGTGG TTGTCTGCAT TTTTGGAGAC
    ATGACATCGG CACTGATTGG AGTTCGGAAT TTGGTGATGC CACTGAGAGC CAGCAATTTT
    CATTACCATG AGTTGAAACA TATAGTCTTT GTTGGGTCCC TTGATTACCT TAAAAGAGAA
    TGGGAAACAC TACACAACTT CCCAAAGGTT TCCATCTTGC CTGGCACACC ATTAAGTCGA
    GCAGATCTGA GAGCTGTCAA CATTAACCTG TGTGACATGT GCGTTATTCT GTCAGCCAAT
    CAGAATAATA TTGATGATAC ATCACTGCAG GACAAAGAAT GCATTTTGGC ATCTCTCAAC
    ATCAAATCCA TGCAGTTTGA TGACAGCATA GGGCTCTTGC AAGCTAACTC TCAAGGGTTT
    ACGCCCCCAG GAATGGAGAG ATCCTCACCT GATAATAGCC CAGTGCATGG TGTTGCAAGA
    CAGGCATCCA TAACTACAGG GGCCAACATT CCCATAATTA CAGAACTTGT GAACGACTCA
    AATGTTCAGT TCTTGGACCA GGATGATGAT GATGACCCAG ATACAGAGTT ATACTTGACT
    CAGCCCTTCG CCTGTGGGAC CGCCTTTGCT GTCAGTGTGT TAGACTCCCT TATGAGTGCA
    ACATACTTCA ATGATAACAT TCTGACTCTA ATCAGAACTC TGGTGACTGG GGGAGCAACA
    CCAGAGCTGG AAGCCCTCAT TGCAGAAGAG AACGCTCTGC GTGGGGGTTA TAGCACCCCA
    CAAACTCTAG CAAACAGAGA CCGCTGTCGA GTAGCCCAAT TAGCCTTGTA TGATGGACCA
    TTTGCAGATT TGGGGGATGG TGGCTGTTAT GGAGACCTTT ACTGCAAAGC ATTAAAAACT
    TACAACATGC TGTGCTTTGG TATATACCGA CTCCGAGATG CCCACCTCAG CACACCCAGC
    CAGTGTACCA AAAGGTACGT TATCACCAAC CCTCCATATG AGTTTGAACT GGTTCCCACA
    GATCTCATCT TTTGCCTGAT GCAGTTCGAC CATAATGCCA GCCAGTCCCG AGCTAGCCTG
    TCTCACTCTT CACACTCCTC GCACTCATCT AGCAAAAAAA GTTCATCTGT TACCTCCATT
    CTACACACAG CCTCAGCCAA CCGTCAGAAC AGAGTCAAGG CTCGAGATTC CCGTGACAAA
    CAAAAGACGA ATAGAATGGG CCAAGCAGAA AAGAAATGGT ATACAGATGA AACGGAAAAC
    ACTTATCCCA GAAACATTCA GATTAAGCCA ATGAGCACAC ATATGGCAAA TCAGATTAAT
    CAATACAAAT CAACAAGCAG CTTGATACCG CCAATACGAG AGGTCGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35194
    Clone ID Related Accession (Same CDS sequence) XM_012966939.2 , XM_012966939.3
    Accession Version XM_012966939.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966939.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GCTAAGCCTG GCAAACTGCT GCCTTTGATT TCATTCAGTC AGGAAAAAAA CAGTGGGACA
    CACATACCAA TTATTACAGA GTTGGTGAAC GACTCAAATG TTCAGTTCTT GGACCAGGAT
    GATGATGATG ACCCAGATAC AGAGTTATAC TTGACTCAGC CCTTCGCCTG TGGGACCGCC
    TTTGCTGTCA GTGTGTTAGA CTCCCTTATG AGTGCAACAT ACTTCAATGA TAACATTCTG
    ACTCTAATCA GAACTCTGGT GACTGGGGGA GCAACACCAG AGCTGGAAGC CCTCATTGCA
    GAAGAGAACG CTCTGCGTGG GGGTTATAGC ACCCCACAAA CTCTAGCAAA CAGAGACCGC
    TGTCGAGTAG CCCAATTAGC CTTGTATGAT GGACCATTTG CAGATTTGGG GGATGGTGGC
    TGTTATGGAG ACCTTTACTG CAAAGCATTA AAAACTTACA ACATGCTGTG CTTTGGTATA
    TACCGACTCC GAGATGCCCA CCTCAGCACA CCCAGCCAGT GTACCAAAAG GTACGTTATC
    ACCAACCCTC CATATGAGTT TGAACTGGTT CCCACAGATC TCATCTTTTG CCTGATGCAG
    TTCGACCATA ATGCCAGCCA GTCCCGAGCT AGCCTGTCTC ACTCTTCACA CTCCTCGCAC
    TCATCTAGCA AAAAAAGTTC ATCTGTTACC TCCATTCTAC ACACAGCCTC AGCCAACCGT
    CAGAACAGAG TCAAGGCTCG AGATTCCCGT GACAAACAAA AGACGAATAG AATGGGCCAA
    GCAGAAAAGA AATGGTATAC AGATGAAACG GAAAACACTT ATCCCAGAAA CATTCAGATT
    AAGCCAATGA GCACACATAT GGCAAATCAG ATTAATCAAT ACAAATCAAC AAGCAGCTTG
    ATACCGCCAA TACGAGAGGT CGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822393.1
    CDS1051..4746
    Translation

    Target ORF information:

    RefSeq Version XM_012966939.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966939.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GCTAAGCCTG GCAAACTGCT GCCTTTGATT TCATTCAGTC AGGAAAAAAA CAGTGGGACA
    CACATACCAA TTATTACAGA GTTGGTGAAC GACTCAAATG TTCAGTTCTT GGACCAGGAT
    GATGATGATG ACCCAGATAC AGAGTTATAC TTGACTCAGC CCTTCGCCTG TGGGACCGCC
    TTTGCTGTCA GTGTGTTAGA CTCCCTTATG AGTGCAACAT ACTTCAATGA TAACATTCTG
    ACTCTAATCA GAACTCTGGT GACTGGGGGA GCAACACCAG AGCTGGAAGC CCTCATTGCA
    GAAGAGAACG CTCTGCGTGG GGGTTATAGC ACCCCACAAA CTCTAGCAAA CAGAGACCGC
    TGTCGAGTAG CCCAATTAGC CTTGTATGAT GGACCATTTG CAGATTTGGG GGATGGTGGC
    TGTTATGGAG ACCTTTACTG CAAAGCATTA AAAACTTACA ACATGCTGTG CTTTGGTATA
    TACCGACTCC GAGATGCCCA CCTCAGCACA CCCAGCCAGT GTACCAAAAG GTACGTTATC
    ACCAACCCTC CATATGAGTT TGAACTGGTT CCCACAGATC TCATCTTTTG CCTGATGCAG
    TTCGACCATA ATGCCAGCCA GTCCCGAGCT AGCCTGTCTC ACTCTTCACA CTCCTCGCAC
    TCATCTAGCA AAAAAAGTTC ATCTGTTACC TCCATTCTAC ACACAGCCTC AGCCAACCGT
    CAGAACAGAG TCAAGGCTCG AGATTCCCGT GACAAACAAA AGACGAATAG AATGGGCCAA
    GCAGAAAAGA AATGGTATAC AGATGAAACG GAAAACACTT ATCCCAGAAA CATTCAGATT
    AAGCCAATGA GCACACATAT GGCAAATCAG ATTAATCAAT ACAAATCAAC AAGCAGCTTG
    ATACCGCCAA TACGAGAGGT CGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35195
    Clone ID Related Accession (Same CDS sequence) XM_012966940.3 , XM_012966940.2
    Accession Version XM_012966940.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966940.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GTGAACGACT CAAATGTTCA GTTCTTGGAC CAGGATGATG ATGATGACCC AGATACAGAG
    TTATACTTGA CTCAGCCCTT CGCCTGTGGG ACCGCCTTTG CTGTCAGTGT GTTAGACTCC
    CTTATGAGTG CAACATACTT CAATGATAAC ATTCTGACTC TAATCAGAAC TCTGGTGACT
    GGGGGAGCAA CACCAGAGCT GGAAGCCCTC ATTGCAGAAG AGAACGCTCT GCGTGGGGGT
    TATAGCACCC CACAAACTCT AGCAAACAGA GACCGCTGTC GAGTAGCCCA ATTAGCCTTG
    TATGATGGAC CATTTGCAGA TTTGGGGGAT GGTGGCTGTT ATGGAGACCT TTACTGCAAA
    GCATTAAAAA CTTACAACAT GCTGTGCTTT GGTATATACC GACTCCGAGA TGCCCACCTC
    AGCACACCCA GCCAGTGTAC CAAAAGGTAC GTTATCACCA ACCCTCCATA TGAGTTTGAA
    CTGGTTCCCA CAGATCTCAT CTTTTGCCTG ATGCAGTTCG ACCATAATGC CAGCCAGTCC
    CGAGCTAGCC TGTCTCACTC TTCACACTCC TCGCACTCAT CTAGCAAAAA AAGTTCATCT
    GTTACCTCCA TTCTACACAC AGCCTCAGCC AACCGTCAGA ACAGAGTCAA GGCTCGAGAT
    TCCCGTGACA AACAAAAGAC GAATAGAATG GGCCAAGCAG AAAAGAAATG GTATACAGAT
    GAAACGGAAA ACACTTATCC CAGAAACATT CAGATTAAGC CAATGAGCAC ACATATGGCA
    AATCAGATTA ATCAATACAA ATCAACAAGC AGCTTGATAC CGCCAATACG AGAGGTCGAA
    GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822394.1
    CDS1046..4657
    Translation

    Target ORF information:

    RefSeq Version XM_012966940.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966940.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GTGAACGACT CAAATGTTCA GTTCTTGGAC CAGGATGATG ATGATGACCC AGATACAGAG
    TTATACTTGA CTCAGCCCTT CGCCTGTGGG ACCGCCTTTG CTGTCAGTGT GTTAGACTCC
    CTTATGAGTG CAACATACTT CAATGATAAC ATTCTGACTC TAATCAGAAC TCTGGTGACT
    GGGGGAGCAA CACCAGAGCT GGAAGCCCTC ATTGCAGAAG AGAACGCTCT GCGTGGGGGT
    TATAGCACCC CACAAACTCT AGCAAACAGA GACCGCTGTC GAGTAGCCCA ATTAGCCTTG
    TATGATGGAC CATTTGCAGA TTTGGGGGAT GGTGGCTGTT ATGGAGACCT TTACTGCAAA
    GCATTAAAAA CTTACAACAT GCTGTGCTTT GGTATATACC GACTCCGAGA TGCCCACCTC
    AGCACACCCA GCCAGTGTAC CAAAAGGTAC GTTATCACCA ACCCTCCATA TGAGTTTGAA
    CTGGTTCCCA CAGATCTCAT CTTTTGCCTG ATGCAGTTCG ACCATAATGC CAGCCAGTCC
    CGAGCTAGCC TGTCTCACTC TTCACACTCC TCGCACTCAT CTAGCAAAAA AAGTTCATCT
    GTTACCTCCA TTCTACACAC AGCCTCAGCC AACCGTCAGA ACAGAGTCAA GGCTCGAGAT
    TCCCGTGACA AACAAAAGAC GAATAGAATG GGCCAAGCAG AAAAGAAATG GTATACAGAT
    GAAACGGAAA ACACTTATCC CAGAAACATT CAGATTAAGC CAATGAGCAC ACATATGGCA
    AATCAGATTA ATCAATACAA ATCAACAAGC AGCTTGATAC CGCCAATACG AGAGGTCGAA
    GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35197
    Clone ID Related Accession (Same CDS sequence) XM_012966942.2 , XM_012966942.3
    Accession Version XM_012966942.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3777bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966942.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCC AAGATGTCCA CCCGCAAGAG GCTGATACAG
    GCATGTTGTA TGGGGTGCTC TGAGATAGAC TGCTCTTGCA TGTCAGGCAC TTTGCGTAGT
    AACATGGGAA CCCTTGAACA AGCCTTCCCA ATTTCTCCTG TCTCTGTTAA TGATTTCTCC
    ACCAGTTTAC GTGGCTTTGA AGATGAGCAA CCATCAGCCT TGTCACCAAA AAAAAAGCAA
    CGAAATGGAG GAATGAGGCA ATCTCCAAAC TCTTCACCTA ACATGATGAG GCATGATCCC
    CTTCTTATGA CTGGAAATGA TCAAATTGAT AATATGGATT CTAGCAGTGT TAAAAGATAT
    GATTCTACTG GTATGTTCCA CTGGTGTCCA GCCAAGGAAT TGGATAAAGT GCTTCTGACA
    CGGAGTGAAG CTGCCATGAC AGTCCTCAGT GGGCATGTGG TTGTCTGCAT TTTTGGAGAC
    ATGACATCGG CACTGATTGG AGTTCGGAAT TTGGTGATGC CACTGAGAGC CAGCAATTTT
    CATTACCATG AGTTGAAACA TATAGTCTTT GTTGGGTCCC TTGATTACCT TAAAAGAGAA
    TGGGAAACAC TACACAACTT CCCAAAGGTT TCCATCTTGC CTGGCACACC ATTAAGTCGA
    GCAGATCTGA GAGCTGTCAA CATTAACCTG TGTGACATGT GCGTTATTCT GTCAGCCAAT
    CAGAATAATA TTGATGATAC ATCACTGCAG GACAAAGAAT GCATTTTGGC ATCTCTCAAC
    ATCAAATCCA TGCAGTTTGA TGACAGCATA GGGCTCTTGC AAGCTAACTC TCAAGGGTTT
    ACGCCCCCAG GAATGGAGAG ATCCTCACCT GATAATAGCC CAGTGCATGG TGTTGCAAGA
    CAGGCATCCA TAACTACAGG GGCCAACATT CCCATAATTA CAGAACTTGT GAACGACTCA
    AATGTTCAGT TCTTGGACCA GGATGATGAT GATGACCCAG ATACAGAGTT ATACTTGACT
    CAGCCCTTCG CCTGTGGGAC CGCCTTTGCT GTCAGTGTGT TAGACTCCCT TATGAGTGCA
    ACATACTTCA ATGATAACAT TCTGACTCTA ATCAGAACTC TGGTGACTGG GGGAGCAACA
    CCAGAGCTGG AAGCCCTCAT TGCAGAAGAG AACGCTCTGC GTGGGGGTTA TAGCACCCCA
    CAAACTCTAG CAAACAGAGA CCGCTGTCGA GTAGCCCAAT TAGCCTTGTA TGATGGACCA
    TTTGCAGATT TGGGGGATGG TGGCTGTTAT GGAGACCTTT ACTGCAAAGC ATTAAAAACT
    TACAACATGC TGTGCTTTGG TATATACCGA CTCCGAGATG CCCACCTCAG CACACCCAGC
    CAGTGTACCA AAAGGTACGT TATCACCAAC CCTCCATATG AGTTTGAACT GGTTCCCACA
    GATCTCATCT TTTGCCTGAT GCAGTTCGAC CATAATGCCA GCCAGTCCCG AGCTAGCCTG
    TCTCACTCTT CACACTCCTC GCACTCATCT AGCAAAAAAA GTTCATCTGT TACCTCCATT
    CTACACACAG CCTCAGCCAA CCGTCAGAAC AGAGTCAAGG CTCGAGATTC CCGTGACAAA
    CAAAAGACGA ATAGAATGGG CCAAGAAAAG AAATGGTATA CAGATGAAAC GGAAAACACT
    TATCCCAGAA ACATTCAGAT TAAGCCAATG AGCACACATA TGGCAAATCA GATTAATCAA
    TACAAATCAA CAAGCAGCTT GATACCGCCA ATACGAGAGG TCGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822396.1
    CDS1051..4827
    Translation

    Target ORF information:

    RefSeq Version XM_012966942.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966942.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCC AAGATGTCCA CCCGCAAGAG GCTGATACAG
    GCATGTTGTA TGGGGTGCTC TGAGATAGAC TGCTCTTGCA TGTCAGGCAC TTTGCGTAGT
    AACATGGGAA CCCTTGAACA AGCCTTCCCA ATTTCTCCTG TCTCTGTTAA TGATTTCTCC
    ACCAGTTTAC GTGGCTTTGA AGATGAGCAA CCATCAGCCT TGTCACCAAA AAAAAAGCAA
    CGAAATGGAG GAATGAGGCA ATCTCCAAAC TCTTCACCTA ACATGATGAG GCATGATCCC
    CTTCTTATGA CTGGAAATGA TCAAATTGAT AATATGGATT CTAGCAGTGT TAAAAGATAT
    GATTCTACTG GTATGTTCCA CTGGTGTCCA GCCAAGGAAT TGGATAAAGT GCTTCTGACA
    CGGAGTGAAG CTGCCATGAC AGTCCTCAGT GGGCATGTGG TTGTCTGCAT TTTTGGAGAC
    ATGACATCGG CACTGATTGG AGTTCGGAAT TTGGTGATGC CACTGAGAGC CAGCAATTTT
    CATTACCATG AGTTGAAACA TATAGTCTTT GTTGGGTCCC TTGATTACCT TAAAAGAGAA
    TGGGAAACAC TACACAACTT CCCAAAGGTT TCCATCTTGC CTGGCACACC ATTAAGTCGA
    GCAGATCTGA GAGCTGTCAA CATTAACCTG TGTGACATGT GCGTTATTCT GTCAGCCAAT
    CAGAATAATA TTGATGATAC ATCACTGCAG GACAAAGAAT GCATTTTGGC ATCTCTCAAC
    ATCAAATCCA TGCAGTTTGA TGACAGCATA GGGCTCTTGC AAGCTAACTC TCAAGGGTTT
    ACGCCCCCAG GAATGGAGAG ATCCTCACCT GATAATAGCC CAGTGCATGG TGTTGCAAGA
    CAGGCATCCA TAACTACAGG GGCCAACATT CCCATAATTA CAGAACTTGT GAACGACTCA
    AATGTTCAGT TCTTGGACCA GGATGATGAT GATGACCCAG ATACAGAGTT ATACTTGACT
    CAGCCCTTCG CCTGTGGGAC CGCCTTTGCT GTCAGTGTGT TAGACTCCCT TATGAGTGCA
    ACATACTTCA ATGATAACAT TCTGACTCTA ATCAGAACTC TGGTGACTGG GGGAGCAACA
    CCAGAGCTGG AAGCCCTCAT TGCAGAAGAG AACGCTCTGC GTGGGGGTTA TAGCACCCCA
    CAAACTCTAG CAAACAGAGA CCGCTGTCGA GTAGCCCAAT TAGCCTTGTA TGATGGACCA
    TTTGCAGATT TGGGGGATGG TGGCTGTTAT GGAGACCTTT ACTGCAAAGC ATTAAAAACT
    TACAACATGC TGTGCTTTGG TATATACCGA CTCCGAGATG CCCACCTCAG CACACCCAGC
    CAGTGTACCA AAAGGTACGT TATCACCAAC CCTCCATATG AGTTTGAACT GGTTCCCACA
    GATCTCATCT TTTGCCTGAT GCAGTTCGAC CATAATGCCA GCCAGTCCCG AGCTAGCCTG
    TCTCACTCTT CACACTCCTC GCACTCATCT AGCAAAAAAA GTTCATCTGT TACCTCCATT
    CTACACACAG CCTCAGCCAA CCGTCAGAAC AGAGTCAAGG CTCGAGATTC CCGTGACAAA
    CAAAAGACGA ATAGAATGGG CCAAGAAAAG AAATGGTATA CAGATGAAAC GGAAAACACT
    TATCCCAGAA ACATTCAGAT TAAGCCAATG AGCACACATA TGGCAAATCA GATTAATCAA
    TACAAATCAA CAAGCAGCTT GATACCGCCA ATACGAGAGG TCGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35200
    Clone ID Related Accession (Same CDS sequence) XM_012966946.2 , XM_012966946.3
    Accession Version XM_012966946.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966946.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GTGAACGACT CAAATGTTCA GTTCTTGGAC CAGGATGATG ATGATGACCC AGATACAGAG
    TTATACTTGA CTCAGCCCTT CGCCTGTGGG ACCGCCTTTG CTGTCAGTGT GTTAGACTCC
    CTTATGAGTG CAACATACTT CAATGATAAC ATTCTGACTC TAATCAGAAC TCTGGTGACT
    GGGGGAGCAA CACCAGAGCT GGAAGCCCTC ATTGCAGAAG AGAACGCTCT GCGTGGGGGT
    TATAGCACCC CACAAACTCT AGCAAACAGA GACCGCTGTC GAGTAGCCCA ATTAGCCTTG
    TATGATGGAC CATTTGCAGA TTTGGGGGAT GGTGGCTGTT ATGGAGACCT TTACTGCAAA
    GCATTAAAAA CTTACAACAT GCTGTGCTTT GGTATATACC GACTCCGAGA TGCCCACCTC
    AGCACACCCA GCCAGTGTAC CAAAAGGTAC GTTATCACCA ACCCTCCATA TGAGTTTGAA
    CTGGTTCCCA CAGATCTCAT CTTTTGCCTG ATGCAGTTCG ACCATAATGC CAGCCAGTCC
    CGAGCTAGCC TGTCTCACTC TTCACACTCC TCGCACTCAT CTAGCAAAAA AAGTTCATCT
    GTTACCTCCA TTCTACACAC AGCCTCAGCC AACCGTCAGA ACAGAGTCAA GGCTCGAGAT
    TCCCGTGACA AACAAAAGAC GAATAGAATG GGCCAAGAAA AGAAATGGTA TACAGATGAA
    ACGGAAAACA CTTATCCCAG AAACATTCAG ATTAAGCCAA TGAGCACACA TATGGCAAAT
    CAGATTAATC AATACAAATC AACAAGCAGC TTGATACCGC CAATACGAGA GGTCGAAGAT
    GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822400.1
    CDS1046..4654
    Translation

    Target ORF information:

    RefSeq Version XM_012966946.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966946.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GTGAACGACT CAAATGTTCA GTTCTTGGAC CAGGATGATG ATGATGACCC AGATACAGAG
    TTATACTTGA CTCAGCCCTT CGCCTGTGGG ACCGCCTTTG CTGTCAGTGT GTTAGACTCC
    CTTATGAGTG CAACATACTT CAATGATAAC ATTCTGACTC TAATCAGAAC TCTGGTGACT
    GGGGGAGCAA CACCAGAGCT GGAAGCCCTC ATTGCAGAAG AGAACGCTCT GCGTGGGGGT
    TATAGCACCC CACAAACTCT AGCAAACAGA GACCGCTGTC GAGTAGCCCA ATTAGCCTTG
    TATGATGGAC CATTTGCAGA TTTGGGGGAT GGTGGCTGTT ATGGAGACCT TTACTGCAAA
    GCATTAAAAA CTTACAACAT GCTGTGCTTT GGTATATACC GACTCCGAGA TGCCCACCTC
    AGCACACCCA GCCAGTGTAC CAAAAGGTAC GTTATCACCA ACCCTCCATA TGAGTTTGAA
    CTGGTTCCCA CAGATCTCAT CTTTTGCCTG ATGCAGTTCG ACCATAATGC CAGCCAGTCC
    CGAGCTAGCC TGTCTCACTC TTCACACTCC TCGCACTCAT CTAGCAAAAA AAGTTCATCT
    GTTACCTCCA TTCTACACAC AGCCTCAGCC AACCGTCAGA ACAGAGTCAA GGCTCGAGAT
    TCCCGTGACA AACAAAAGAC GAATAGAATG GGCCAAGAAA AGAAATGGTA TACAGATGAA
    ACGGAAAACA CTTATCCCAG AAACATTCAG ATTAAGCCAA TGAGCACACA TATGGCAAAT
    CAGATTAATC AATACAAATC AACAAGCAGC TTGATACCGC CAATACGAGA GGTCGAAGAT
    GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45786
    Clone ID Related Accession (Same CDS sequence) XM_018095895.1
    Accession Version XM_018095895.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCC AAGATGTCCA CCCGCAAGAG GCTGATACAG
    GCATGTTGTA TGGGGTGCTC TGAGATAGAC TGCTCTTGCA TGTCAGGCAC TTTGCGTAGT
    AACATGGGAA CCCTTGAACA AGCCTTCCCA ATTTCTCCTG TCTCTGTTAA TGATTTCTCC
    ACCAGTTTAC GTGGCTTTGA AGATGAGCAA CCATCAGCCT TGTCACCAAA AAAAAAGCAA
    CGAAATGGAG GAATGAGGCA ATCTCCAAAC TCTTCACCTA ACATGATGAG GCATGATCCC
    CTTCTTATGA CTGGAAATGA TCAAATTGAT AATATGGATT CTAGCAGTGT TAAAAGATAT
    GATTCTACTG GTATGTTCCA CTGGTGTCCA GCCAAGGAAT TGGATAAAGT GCTTCTGACA
    CGGAGTGAAG CTGCCATGAC AGTCCTCAGT GGGCATGTGG TTGTCTGCAT TTTTGGAGAC
    ATGACATCGG CACTGATTGG AGTTCGGAAT TTGGTGATGC CACTGAGAGC CAGCAATTTT
    CATTACCATG AGTTGAAACA TATAGTCTTT GTTGGGTCCC TTGATTACCT TAAAAGAGAA
    TGGGAAACAC TACACAACTT CCCAAAGGTT TCCATCTTGC CTGGCACACC ATTAAGTCGA
    GCAGATCTGA GAGCTGTCAA CATTAACCTG TGTGACATGT GCGTTATTCT GTCAGCCAAT
    CAGAATAATA TTGATGATAC ATCACTGCAG GACAAAGAAT GCATTTTGGC ATCTCTCAAC
    ATCAAATCCA TGCAGTTTGA TGACAGCATA GGGCTCTTGC AAGCTAACTC TCAAGGGTTT
    ACGCCCCCAG GAATGGAGAG ATCCTCACCT GATAATAGCC CAGTGCATGG TGTTGCAAGA
    CAGGCATCCA TAACTACAGG GGCCAACATT CCCATAATTA CAGAACTTGC TAAGCCTGGC
    AAACTGCTGC CTTTGATTTC ATTCAGTCAG GAAAAAAACA GTGGGACACA CATACCAATT
    ATTACAGAGT TGGTGAACGA CTCAAATGTT CAGTTCTTGG ACCAGGATGA TGATGATGAC
    CCAGATACAG AGTTATACTT GACTCAGCCC TTCGCCTGTG GGACCGCCTT TGCTGTCAGT
    GTGTTAGACT CCCTTATGAG TGCAACATAC TTCAATGATA ACATTCTGAC TCTAATCAGA
    ACTCTGGTGA CTGGGGGAGC AACACCAGAG CTGGAAGCCC TCATTGCAGA AGAGAACGCT
    CTGCGTGGGG GTTATAGCAC CCCACAAACT CTAGCAAACA GAGACCGCTG TCGAGTAGCC
    CAATTAGCCT TGTATGATGG ACCATTTGCA GATTTGGGGG ATGGTGGCTG TTATGGAGAC
    CTTTACTGCA AAGCATTAAA AACTTACAAC ATGCTGTGCT TTGGTATATA CCGACTCCGA
    GATGCCCACC TCAGCACACC CAGCCAGTGT ACCAAAAGGT ACGTTATCAC CAACCCTCCA
    TATGAGTTTG AACTGGTTCC CACAGATCTC ATCTTTTGCC TGATGCAGTT CGACCATAAT
    GCCAGCCAGT CCCGAGCTAG CCTGTCTCAC TCTTCACACT CCTCGCACTC ATCTAGCAAA
    AAAAGTTCAT CTGTTACCTC CATTCTACAC ACAGCCTCAG CCAACCGTCA GAACAGAGTC
    AAGGCTCGAG ATTCCCGTGA CAAACAAAAG ACGAATAGAA TGGGCCAAGC AGAAAAGAAA
    TGGTATACAG ATGAAACGGA AAACACTTAT CCCAGAAACA TTCAGATTAA GCCAATGAGC
    ACACATATGG CAAATCAGAT TAATCAATAC AAATCAACAA GCAGCTTGAT ACCGCCAATA
    CGAGAGGTCG AAGATGAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951384.1
    CDS1501..5364
    Translation

    Target ORF information:

    RefSeq Version XM_018095895.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095895.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCC AAGATGTCCA CCCGCAAGAG GCTGATACAG
    GCATGTTGTA TGGGGTGCTC TGAGATAGAC TGCTCTTGCA TGTCAGGCAC TTTGCGTAGT
    AACATGGGAA CCCTTGAACA AGCCTTCCCA ATTTCTCCTG TCTCTGTTAA TGATTTCTCC
    ACCAGTTTAC GTGGCTTTGA AGATGAGCAA CCATCAGCCT TGTCACCAAA AAAAAAGCAA
    CGAAATGGAG GAATGAGGCA ATCTCCAAAC TCTTCACCTA ACATGATGAG GCATGATCCC
    CTTCTTATGA CTGGAAATGA TCAAATTGAT AATATGGATT CTAGCAGTGT TAAAAGATAT
    GATTCTACTG GTATGTTCCA CTGGTGTCCA GCCAAGGAAT TGGATAAAGT GCTTCTGACA
    CGGAGTGAAG CTGCCATGAC AGTCCTCAGT GGGCATGTGG TTGTCTGCAT TTTTGGAGAC
    ATGACATCGG CACTGATTGG AGTTCGGAAT TTGGTGATGC CACTGAGAGC CAGCAATTTT
    CATTACCATG AGTTGAAACA TATAGTCTTT GTTGGGTCCC TTGATTACCT TAAAAGAGAA
    TGGGAAACAC TACACAACTT CCCAAAGGTT TCCATCTTGC CTGGCACACC ATTAAGTCGA
    GCAGATCTGA GAGCTGTCAA CATTAACCTG TGTGACATGT GCGTTATTCT GTCAGCCAAT
    CAGAATAATA TTGATGATAC ATCACTGCAG GACAAAGAAT GCATTTTGGC ATCTCTCAAC
    ATCAAATCCA TGCAGTTTGA TGACAGCATA GGGCTCTTGC AAGCTAACTC TCAAGGGTTT
    ACGCCCCCAG GAATGGAGAG ATCCTCACCT GATAATAGCC CAGTGCATGG TGTTGCAAGA
    CAGGCATCCA TAACTACAGG GGCCAACATT CCCATAATTA CAGAACTTGC TAAGCCTGGC
    AAACTGCTGC CTTTGATTTC ATTCAGTCAG GAAAAAAACA GTGGGACACA CATACCAATT
    ATTACAGAGT TGGTGAACGA CTCAAATGTT CAGTTCTTGG ACCAGGATGA TGATGATGAC
    CCAGATACAG AGTTATACTT GACTCAGCCC TTCGCCTGTG GGACCGCCTT TGCTGTCAGT
    GTGTTAGACT CCCTTATGAG TGCAACATAC TTCAATGATA ACATTCTGAC TCTAATCAGA
    ACTCTGGTGA CTGGGGGAGC AACACCAGAG CTGGAAGCCC TCATTGCAGA AGAGAACGCT
    CTGCGTGGGG GTTATAGCAC CCCACAAACT CTAGCAAACA GAGACCGCTG TCGAGTAGCC
    CAATTAGCCT TGTATGATGG ACCATTTGCA GATTTGGGGG ATGGTGGCTG TTATGGAGAC
    CTTTACTGCA AAGCATTAAA AACTTACAAC ATGCTGTGCT TTGGTATATA CCGACTCCGA
    GATGCCCACC TCAGCACACC CAGCCAGTGT ACCAAAAGGT ACGTTATCAC CAACCCTCCA
    TATGAGTTTG AACTGGTTCC CACAGATCTC ATCTTTTGCC TGATGCAGTT CGACCATAAT
    GCCAGCCAGT CCCGAGCTAG CCTGTCTCAC TCTTCACACT CCTCGCACTC ATCTAGCAAA
    AAAAGTTCAT CTGTTACCTC CATTCTACAC ACAGCCTCAG CCAACCGTCA GAACAGAGTC
    AAGGCTCGAG ATTCCCGTGA CAAACAAAAG ACGAATAGAA TGGGCCAAGC AGAAAAGAAA
    TGGTATACAG ATGAAACGGA AAACACTTAT CCCAGAAACA TTCAGATTAA GCCAATGAGC
    ACACATATGG CAAATCAGAT TAATCAATAC AAATCAACAA GCAGCTTGAT ACCGCCAATA
    CGAGAGGTCG AAGATGAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45787
    Clone ID Related Accession (Same CDS sequence) XM_018095896.1
    Accession Version XM_018095896.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GCTAAGCCTG GCAAACTGCT GCCTTTGATT TCATTCAGTC AGGAAAAAAA CAGTGGGACA
    CACATACCAA TTATTACAGA GTTGGTGAAC GACTCAAATG TTCAGTTCTT GGACCAGGAT
    GATGATGATG ACCCAGATAC AGAGTTATAC TTGACTCAGC CCTTCGCCTG TGGGACCGCC
    TTTGCTGTCA GTGTGTTAGA CTCCCTTATG AGTGCAACAT ACTTCAATGA TAACATTCTG
    ACTCTAATCA GAACTCTGGT GACTGGGGGA GCAACACCAG AGCTGGAAGC CCTCATTGCA
    GAAGAGAACG CTCTGCGTGG GGGTTATAGC ACCCCACAAA CTCTAGCAAA CAGAGACCGC
    TGTCGAGTAG CCCAATTAGC CTTGTATGAT GGACCATTTG CAGATTTGGG GGATGGTGGC
    TGTTATGGAG ACCTTTACTG CAAAGCATTA AAAACTTACA ACATGCTGTG CTTTGGTATA
    TACCGACTCC GAGATGCCCA CCTCAGCACA CCCAGCCAGT GTACCAAAAG GTACGTTATC
    ACCAACCCTC CATATGAGTT TGAACTGGTT CCCACAGATC TCATCTTTTG CCTGATGCAG
    TTCGACCATA ATGCCAGCCA GTCCCGAGCT AGCCTGTCTC ACTCTTCACA CTCCTCGCAC
    TCATCTAGCA AAAAAAGTTC ATCTGTTACC TCCATTCTAC ACACAGCCTC AGCCAACCGT
    CAGAACAGAG TCAAGGCTCG AGATTCCCGT GACAAACAAA AGACGAATAG AATGGGCCAA
    GAAAAGAAAT GGTATACAGA TGAAACGGAA AACACTTATC CCAGAAACAT TCAGATTAAG
    CCAATGAGCA CACATATGGC AAATCAGATT AATCAATACA AATCAACAAG CAGCTTGATA
    CCGCCAATAC GAGAGGTCGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951385.1
    CDS1499..5191
    Translation

    Target ORF information:

    RefSeq Version XM_018095896.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095896.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GCTAAGCCTG GCAAACTGCT GCCTTTGATT TCATTCAGTC AGGAAAAAAA CAGTGGGACA
    CACATACCAA TTATTACAGA GTTGGTGAAC GACTCAAATG TTCAGTTCTT GGACCAGGAT
    GATGATGATG ACCCAGATAC AGAGTTATAC TTGACTCAGC CCTTCGCCTG TGGGACCGCC
    TTTGCTGTCA GTGTGTTAGA CTCCCTTATG AGTGCAACAT ACTTCAATGA TAACATTCTG
    ACTCTAATCA GAACTCTGGT GACTGGGGGA GCAACACCAG AGCTGGAAGC CCTCATTGCA
    GAAGAGAACG CTCTGCGTGG GGGTTATAGC ACCCCACAAA CTCTAGCAAA CAGAGACCGC
    TGTCGAGTAG CCCAATTAGC CTTGTATGAT GGACCATTTG CAGATTTGGG GGATGGTGGC
    TGTTATGGAG ACCTTTACTG CAAAGCATTA AAAACTTACA ACATGCTGTG CTTTGGTATA
    TACCGACTCC GAGATGCCCA CCTCAGCACA CCCAGCCAGT GTACCAAAAG GTACGTTATC
    ACCAACCCTC CATATGAGTT TGAACTGGTT CCCACAGATC TCATCTTTTG CCTGATGCAG
    TTCGACCATA ATGCCAGCCA GTCCCGAGCT AGCCTGTCTC ACTCTTCACA CTCCTCGCAC
    TCATCTAGCA AAAAAAGTTC ATCTGTTACC TCCATTCTAC ACACAGCCTC AGCCAACCGT
    CAGAACAGAG TCAAGGCTCG AGATTCCCGT GACAAACAAA AGACGAATAG AATGGGCCAA
    GAAAAGAAAT GGTATACAGA TGAAACGGAA AACACTTATC CCAGAAACAT TCAGATTAAG
    CCAATGAGCA CACATATGGC AAATCAGATT AATCAATACA AATCAACAAG CAGCTTGATA
    CCGCCAATAC GAGAGGTCGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62475
    Clone ID Related Accession (Same CDS sequence) XM_031906033.1
    Accession Version XM_031906033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CTACAAGGGA AGAAAATAGA
    TTAAGTATCC ACAGGCTTTC TATTCATCCC CAGACAGCCA AAGCTAGCTA TTCTGTGGCC
    AGTTCCAAAC TCTGCACAGA ACAGCAGGAG CCTGTGCCTC TTGTTAATAA CCGCAAAGGA
    AGCCTGTTCT TGCCATGTGA TTCCTCTCTC CTGCACCTGC AGTTACTGGG TTCCTCTGGC
    ACTGAACACC GTACCAGTAT TAAATTGCAG CGTGCACTGA GCTTGCCAGG CAAATACCGC
    TACCACCCCA ATCAGCCAGT ACTCATCCAA AAGCAGTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG CTAAGCCTGG CAAACTGCTG CCTTTGATTT CATTCAGTCA GGAAAAAAAC
    AGTGGGACAC ACATACCAAT TATTACAGAG TTGGTGAACG ACTCAAATGT TCAGTTCTTG
    GACCAGGATG ATGATGATGA CCCAGATACA GAGTTATACT TGACTCAGCC CTTCGCCTGT
    GGGACCGCCT TTGCTGTCAG TGTGTTAGAC TCCCTTATGA GTGCAACATA CTTCAATGAT
    AACATTCTGA CTCTAATCAG AACTCTGGTG ACTGGGGGAG CAACACCAGA GCTGGAAGCC
    CTCATTGCAG AAGAGAACGC TCTGCGTGGG GGTTATAGCA CCCCACAAAC TCTAGCAAAC
    AGAGACCGCT GTCGAGTAGC CCAATTAGCC TTGTATGATG GACCATTTGC AGATTTGGGG
    GATGGTGGCT GTTATGGAGA CCTTTACTGC AAAGCATTAA AAACTTACAA CATGCTGTGC
    TTTGGTATAT ACCGACTCCG AGATGCCCAC CTCAGCACAC CCAGCCAGTG TACCAAAAGG
    TACGTTATCA CCAACCCTCC ATATGAGTTT GAACTGGTTC CCACAGATCT CATCTTTTGC
    CTGATGCAGT TCGACCATAA TGCCAGCCAG TCCCGAGCTA GCCTGTCTCA CTCTTCACAC
    TCCTCGCACT CATCTAGCAA AAAAAGTTCA TCTGTTACCT CCATTCTACA CACAGCCTCA
    GCCAACCGTC AGAACAGAGT CAAGGCTCGA GATTCCCGTG ACAAACAAAA GACGAATAGA
    ATGGGCCAAG CAGAAAAGAA ATGGTATACA GATGAAACGG AAAACACTTA TCCCAGAAAC
    ATTCAGATTA AGCCAATGAG CACACATATG GCAAATCAGA TTAATCAATA CAAATCAACA
    AGCAGCTTGA TACCGCCAAT ACGAGAGGTC GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761893.1
    CDS1064..5068
    Translation

    Target ORF information:

    RefSeq Version XM_031906033.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CTACAAGGGA AGAAAATAGA
    TTAAGTATCC ACAGGCTTTC TATTCATCCC CAGACAGCCA AAGCTAGCTA TTCTGTGGCC
    AGTTCCAAAC TCTGCACAGA ACAGCAGGAG CCTGTGCCTC TTGTTAATAA CCGCAAAGGA
    AGCCTGTTCT TGCCATGTGA TTCCTCTCTC CTGCACCTGC AGTTACTGGG TTCCTCTGGC
    ACTGAACACC GTACCAGTAT TAAATTGCAG CGTGCACTGA GCTTGCCAGG CAAATACCGC
    TACCACCCCA ATCAGCCAGT ACTCATCCAA AAGCAGTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG CTAAGCCTGG CAAACTGCTG CCTTTGATTT CATTCAGTCA GGAAAAAAAC
    AGTGGGACAC ACATACCAAT TATTACAGAG TTGGTGAACG ACTCAAATGT TCAGTTCTTG
    GACCAGGATG ATGATGATGA CCCAGATACA GAGTTATACT TGACTCAGCC CTTCGCCTGT
    GGGACCGCCT TTGCTGTCAG TGTGTTAGAC TCCCTTATGA GTGCAACATA CTTCAATGAT
    AACATTCTGA CTCTAATCAG AACTCTGGTG ACTGGGGGAG CAACACCAGA GCTGGAAGCC
    CTCATTGCAG AAGAGAACGC TCTGCGTGGG GGTTATAGCA CCCCACAAAC TCTAGCAAAC
    AGAGACCGCT GTCGAGTAGC CCAATTAGCC TTGTATGATG GACCATTTGC AGATTTGGGG
    GATGGTGGCT GTTATGGAGA CCTTTACTGC AAAGCATTAA AAACTTACAA CATGCTGTGC
    TTTGGTATAT ACCGACTCCG AGATGCCCAC CTCAGCACAC CCAGCCAGTG TACCAAAAGG
    TACGTTATCA CCAACCCTCC ATATGAGTTT GAACTGGTTC CCACAGATCT CATCTTTTGC
    CTGATGCAGT TCGACCATAA TGCCAGCCAG TCCCGAGCTA GCCTGTCTCA CTCTTCACAC
    TCCTCGCACT CATCTAGCAA AAAAAGTTCA TCTGTTACCT CCATTCTACA CACAGCCTCA
    GCCAACCGTC AGAACAGAGT CAAGGCTCGA GATTCCCGTG ACAAACAAAA GACGAATAGA
    ATGGGCCAAG CAGAAAAGAA ATGGTATACA GATGAAACGG AAAACACTTA TCCCAGAAAC
    ATTCAGATTA AGCCAATGAG CACACATATG GCAAATCAGA TTAATCAATA CAAATCAACA
    AGCAGCTTGA TACCGCCAAT ACGAGAGGTC GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62476
    Clone ID Related Accession (Same CDS sequence) XM_031906034.1
    Accession Version XM_031906034.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CTACAAGGGA AGAAAATAGA
    TTAAGTATCC ACAGGCTTTC TATTCATCCC CAGACAGCCA AAGCTAGCTA TTCTGTGGCC
    AGTTCCAAAC TCTGCACAGA ACAGCAGGAG CCTGTGCCTC TTGTTAATAA CCGCAAAGGA
    AGCCTGTTCT TGCCATGTGA TTCCTCTCTC CTGCACCTGC AGTTACTGGG TTCCTCTGGC
    ACTGAACACC GTACCAGTAT TAAATTGCAG CGTGCACTGA GCTTGCCAGG CAAATACCGC
    TACCACCCCA ATCAGCCAGT ACTCATCCAA AAGCAGTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG CTAAGCCTGG CAAACTGCTG CCTTTGATTT CATTCAGTCA GGAAAAAAAC
    AGTGGGACAC ACATACCAAT TATTACAGAG TTGGTGAACG ACTCAAATGT TCAGTTCTTG
    GACCAGGATG ATGATGATGA CCCAGATACA GAGTTATACT TGACTCAGCC CTTCGCCTGT
    GGGACCGCCT TTGCTGTCAG TGTGTTAGAC TCCCTTATGA GTGCAACATA CTTCAATGAT
    AACATTCTGA CTCTAATCAG AACTCTGGTG ACTGGGGGAG CAACACCAGA GCTGGAAGCC
    CTCATTGCAG AAGAGAACGC TCTGCGTGGG GGTTATAGCA CCCCACAAAC TCTAGCAAAC
    AGAGACCGCT GTCGAGTAGC CCAATTAGCC TTGTATGATG GACCATTTGC AGATTTGGGG
    GATGGTGGCT GTTATGGAGA CCTTTACTGC AAAGCATTAA AAACTTACAA CATGCTGTGC
    TTTGGTATAT ACCGACTCCG AGATGCCCAC CTCAGCACAC CCAGCCAGTG TACCAAAAGG
    TACGTTATCA CCAACCCTCC ATATGAGTTT GAACTGGTTC CCACAGATCT CATCTTTTGC
    CTGATGCAGT TCGACCATAA TGCCAGCCAG TCCCGAGCTA GCCTGTCTCA CTCTTCACAC
    TCCTCGCACT CATCTAGCAA AAAAAGTTCA TCTGTTACCT CCATTCTACA CACAGCCTCA
    GCCAACCGTC AGAACAGAGT CAAGGCTCGA GATTCCCGTG ACAAACAAAA GACGAATAGA
    ATGGGCCAAG AAAAGAAATG GTATACAGAT GAAACGGAAA ACACTTATCC CAGAAACATT
    CAGATTAAGC CAATGAGCAC ACATATGGCA AATCAGATTA ATCAATACAA ATCAACAAGC
    AGCTTGATAC CGCCAATACG AGAGGTCGAA GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761894.1
    CDS1064..5065
    Translation

    Target ORF information:

    RefSeq Version XM_031906034.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CTACAAGGGA AGAAAATAGA
    TTAAGTATCC ACAGGCTTTC TATTCATCCC CAGACAGCCA AAGCTAGCTA TTCTGTGGCC
    AGTTCCAAAC TCTGCACAGA ACAGCAGGAG CCTGTGCCTC TTGTTAATAA CCGCAAAGGA
    AGCCTGTTCT TGCCATGTGA TTCCTCTCTC CTGCACCTGC AGTTACTGGG TTCCTCTGGC
    ACTGAACACC GTACCAGTAT TAAATTGCAG CGTGCACTGA GCTTGCCAGG CAAATACCGC
    TACCACCCCA ATCAGCCAGT ACTCATCCAA AAGCAGTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG CTAAGCCTGG CAAACTGCTG CCTTTGATTT CATTCAGTCA GGAAAAAAAC
    AGTGGGACAC ACATACCAAT TATTACAGAG TTGGTGAACG ACTCAAATGT TCAGTTCTTG
    GACCAGGATG ATGATGATGA CCCAGATACA GAGTTATACT TGACTCAGCC CTTCGCCTGT
    GGGACCGCCT TTGCTGTCAG TGTGTTAGAC TCCCTTATGA GTGCAACATA CTTCAATGAT
    AACATTCTGA CTCTAATCAG AACTCTGGTG ACTGGGGGAG CAACACCAGA GCTGGAAGCC
    CTCATTGCAG AAGAGAACGC TCTGCGTGGG GGTTATAGCA CCCCACAAAC TCTAGCAAAC
    AGAGACCGCT GTCGAGTAGC CCAATTAGCC TTGTATGATG GACCATTTGC AGATTTGGGG
    GATGGTGGCT GTTATGGAGA CCTTTACTGC AAAGCATTAA AAACTTACAA CATGCTGTGC
    TTTGGTATAT ACCGACTCCG AGATGCCCAC CTCAGCACAC CCAGCCAGTG TACCAAAAGG
    TACGTTATCA CCAACCCTCC ATATGAGTTT GAACTGGTTC CCACAGATCT CATCTTTTGC
    CTGATGCAGT TCGACCATAA TGCCAGCCAG TCCCGAGCTA GCCTGTCTCA CTCTTCACAC
    TCCTCGCACT CATCTAGCAA AAAAAGTTCA TCTGTTACCT CCATTCTACA CACAGCCTCA
    GCCAACCGTC AGAACAGAGT CAAGGCTCGA GATTCCCGTG ACAAACAAAA GACGAATAGA
    ATGGGCCAAG AAAAGAAATG GTATACAGAT GAAACGGAAA ACACTTATCC CAGAAACATT
    CAGATTAAGC CAATGAGCAC ACATATGGCA AATCAGATTA ATCAATACAA ATCAACAAGC
    AGCTTGATAC CGCCAATACG AGAGGTCGAA GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62477
    Clone ID Related Accession (Same CDS sequence) XM_031906035.1
    Accession Version XM_031906035.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CTACAAGGGA AGAAAATAGA
    TTAAGTATCC ACAGGCTTTC TATTCATCCC CAGACAGCCA AAGCTAGCTA TTCTGTGGCC
    AGTTCCAAAC TCTGCACAGA ACAGCAGGAG CCTGTGCCTC TTGTTAATAA CCGCAAAGGA
    AGCCTGTTCT TGCCATGTGA TTCCTCTCTC CTGCACCTGC AGTTACTGGG TTCCTCTGGC
    ACTGAACACC GTACCAGTAT TAAATTGCAG CGTGCACTGA GCTTGCCAGG CAAATACCGC
    TACCACCCCA ATCAGCCAGT ACTCATCCAA AAGCAGTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG TGAACGACTC AAATGTTCAG TTCTTGGACC AGGATGATGA TGATGACCCA
    GATACAGAGT TATACTTGAC TCAGCCCTTC GCCTGTGGGA CCGCCTTTGC TGTCAGTGTG
    TTAGACTCCC TTATGAGTGC AACATACTTC AATGATAACA TTCTGACTCT AATCAGAACT
    CTGGTGACTG GGGGAGCAAC ACCAGAGCTG GAAGCCCTCA TTGCAGAAGA GAACGCTCTG
    CGTGGGGGTT ATAGCACCCC ACAAACTCTA GCAAACAGAG ACCGCTGTCG AGTAGCCCAA
    TTAGCCTTGT ATGATGGACC ATTTGCAGAT TTGGGGGATG GTGGCTGTTA TGGAGACCTT
    TACTGCAAAG CATTAAAAAC TTACAACATG CTGTGCTTTG GTATATACCG ACTCCGAGAT
    GCCCACCTCA GCACACCCAG CCAGTGTACC AAAAGGTACG TTATCACCAA CCCTCCATAT
    GAGTTTGAAC TGGTTCCCAC AGATCTCATC TTTTGCCTGA TGCAGTTCGA CCATAATGCC
    AGCCAGTCCC GAGCTAGCCT GTCTCACTCT TCACACTCCT CGCACTCATC TAGCAAAAAA
    AGTTCATCTG TTACCTCCAT TCTACACACA GCCTCAGCCA ACCGTCAGAA CAGAGTCAAG
    GCTCGAGATT CCCGTGACAA ACAAAAGACG AATAGAATGG GCCAAGCAGA AAAGAAATGG
    TATACAGATG AAACGGAAAA CACTTATCCC AGAAACATTC AGATTAAGCC AATGAGCACA
    CATATGGCAA ATCAGATTAA TCAATACAAA TCAACAAGCA GCTTGATACC GCCAATACGA
    GAGGTCGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761895.1
    CDS1057..4977
    Translation

    Target ORF information:

    RefSeq Version XM_031906035.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906035.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CTACAAGGGA AGAAAATAGA
    TTAAGTATCC ACAGGCTTTC TATTCATCCC CAGACAGCCA AAGCTAGCTA TTCTGTGGCC
    AGTTCCAAAC TCTGCACAGA ACAGCAGGAG CCTGTGCCTC TTGTTAATAA CCGCAAAGGA
    AGCCTGTTCT TGCCATGTGA TTCCTCTCTC CTGCACCTGC AGTTACTGGG TTCCTCTGGC
    ACTGAACACC GTACCAGTAT TAAATTGCAG CGTGCACTGA GCTTGCCAGG CAAATACCGC
    TACCACCCCA ATCAGCCAGT ACTCATCCAA AAGCAGTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG TGAACGACTC AAATGTTCAG TTCTTGGACC AGGATGATGA TGATGACCCA
    GATACAGAGT TATACTTGAC TCAGCCCTTC GCCTGTGGGA CCGCCTTTGC TGTCAGTGTG
    TTAGACTCCC TTATGAGTGC AACATACTTC AATGATAACA TTCTGACTCT AATCAGAACT
    CTGGTGACTG GGGGAGCAAC ACCAGAGCTG GAAGCCCTCA TTGCAGAAGA GAACGCTCTG
    CGTGGGGGTT ATAGCACCCC ACAAACTCTA GCAAACAGAG ACCGCTGTCG AGTAGCCCAA
    TTAGCCTTGT ATGATGGACC ATTTGCAGAT TTGGGGGATG GTGGCTGTTA TGGAGACCTT
    TACTGCAAAG CATTAAAAAC TTACAACATG CTGTGCTTTG GTATATACCG ACTCCGAGAT
    GCCCACCTCA GCACACCCAG CCAGTGTACC AAAAGGTACG TTATCACCAA CCCTCCATAT
    GAGTTTGAAC TGGTTCCCAC AGATCTCATC TTTTGCCTGA TGCAGTTCGA CCATAATGCC
    AGCCAGTCCC GAGCTAGCCT GTCTCACTCT TCACACTCCT CGCACTCATC TAGCAAAAAA
    AGTTCATCTG TTACCTCCAT TCTACACACA GCCTCAGCCA ACCGTCAGAA CAGAGTCAAG
    GCTCGAGATT CCCGTGACAA ACAAAAGACG AATAGAATGG GCCAAGCAGA AAAGAAATGG
    TATACAGATG AAACGGAAAA CACTTATCCC AGAAACATTC AGATTAAGCC AATGAGCACA
    CATATGGCAA ATCAGATTAA TCAATACAAA TCAACAAGCA GCTTGATACC GCCAATACGA
    GAGGTCGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62478
    Clone ID Related Accession (Same CDS sequence) XM_031906036.1
    Accession Version XM_031906036.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TTATTTATTC CAAGATGTCC
    ACCCGCAAGA GGCTGATACA GGCATGTTGT ATGGGGTGCT CTGAGATAGA CTGCTCTTGC
    ATGTCAGGCA CTTTGCGTAG TAACATGGGA ACCCTTGAAC AAGCCTTCCC AATTTCTCCT
    GTCTCTGTTA ATGATTTCTC CACCAGTTTA CGTGGCTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG CTAAGCCTGG CAAACTGCTG CCTTTGATTT CATTCAGTCA GGAAAAAAAC
    AGTGGGACAC ACATACCAAT TATTACAGAG TTGGTGAACG ACTCAAATGT TCAGTTCTTG
    GACCAGGATG ATGATGATGA CCCAGATACA GAGTTATACT TGACTCAGCC CTTCGCCTGT
    GGGACCGCCT TTGCTGTCAG TGTGTTAGAC TCCCTTATGA GTGCAACATA CTTCAATGAT
    AACATTCTGA CTCTAATCAG AACTCTGGTG ACTGGGGGAG CAACACCAGA GCTGGAAGCC
    CTCATTGCAG AAGAGAACGC TCTGCGTGGG GGTTATAGCA CCCCACAAAC TCTAGCAAAC
    AGAGACCGCT GTCGAGTAGC CCAATTAGCC TTGTATGATG GACCATTTGC AGATTTGGGG
    GATGGTGGCT GTTATGGAGA CCTTTACTGC AAAGCATTAA AAACTTACAA CATGCTGTGC
    TTTGGTATAT ACCGACTCCG AGATGCCCAC CTCAGCACAC CCAGCCAGTG TACCAAAAGG
    TACGTTATCA CCAACCCTCC ATATGAGTTT GAACTGGTTC CCACAGATCT CATCTTTTGC
    CTGATGCAGT TCGACCATAA TGCCAGCCAG TCCCGAGCTA GCCTGTCTCA CTCTTCACAC
    TCCTCGCACT CATCTAGCAA AAAAAGTTCA TCTGTTACCT CCATTCTACA CACAGCCTCA
    GCCAACCGTC AGAACAGAGT CAAGGCTCGA GATTCCCGTG ACAAACAAAA GACGAATAGA
    ATGGGCCAAG CAGAAAAGAA ATGGTATACA GATGAAACGG AAAACACTTA TCCCAGAAAC
    ATTCAGATTA AGCCAATGAG CACACATATG GCAAATCAGA TTAATCAATA CAAATCAACA
    AGCAGCTTGA TACCGCCAAT ACGAGAGGTC GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761896.1
    CDS1059..4943
    Translation

    Target ORF information:

    RefSeq Version XM_031906036.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906036.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TTATTTATTC CAAGATGTCC
    ACCCGCAAGA GGCTGATACA GGCATGTTGT ATGGGGTGCT CTGAGATAGA CTGCTCTTGC
    ATGTCAGGCA CTTTGCGTAG TAACATGGGA ACCCTTGAAC AAGCCTTCCC AATTTCTCCT
    GTCTCTGTTA ATGATTTCTC CACCAGTTTA CGTGGCTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG CTAAGCCTGG CAAACTGCTG CCTTTGATTT CATTCAGTCA GGAAAAAAAC
    AGTGGGACAC ACATACCAAT TATTACAGAG TTGGTGAACG ACTCAAATGT TCAGTTCTTG
    GACCAGGATG ATGATGATGA CCCAGATACA GAGTTATACT TGACTCAGCC CTTCGCCTGT
    GGGACCGCCT TTGCTGTCAG TGTGTTAGAC TCCCTTATGA GTGCAACATA CTTCAATGAT
    AACATTCTGA CTCTAATCAG AACTCTGGTG ACTGGGGGAG CAACACCAGA GCTGGAAGCC
    CTCATTGCAG AAGAGAACGC TCTGCGTGGG GGTTATAGCA CCCCACAAAC TCTAGCAAAC
    AGAGACCGCT GTCGAGTAGC CCAATTAGCC TTGTATGATG GACCATTTGC AGATTTGGGG
    GATGGTGGCT GTTATGGAGA CCTTTACTGC AAAGCATTAA AAACTTACAA CATGCTGTGC
    TTTGGTATAT ACCGACTCCG AGATGCCCAC CTCAGCACAC CCAGCCAGTG TACCAAAAGG
    TACGTTATCA CCAACCCTCC ATATGAGTTT GAACTGGTTC CCACAGATCT CATCTTTTGC
    CTGATGCAGT TCGACCATAA TGCCAGCCAG TCCCGAGCTA GCCTGTCTCA CTCTTCACAC
    TCCTCGCACT CATCTAGCAA AAAAAGTTCA TCTGTTACCT CCATTCTACA CACAGCCTCA
    GCCAACCGTC AGAACAGAGT CAAGGCTCGA GATTCCCGTG ACAAACAAAA GACGAATAGA
    ATGGGCCAAG CAGAAAAGAA ATGGTATACA GATGAAACGG AAAACACTTA TCCCAGAAAC
    ATTCAGATTA AGCCAATGAG CACACATATG GCAAATCAGA TTAATCAATA CAAATCAACA
    AGCAGCTTGA TACCGCCAAT ACGAGAGGTC GAAGATGAAT GTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62479
    Clone ID Related Accession (Same CDS sequence) XM_031906037.1
    Accession Version XM_031906037.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CCAAGATGTC CACCCGCAAG
    AGGCTGATAC AGGCATGTTG TATGGGGTGC TCTGAGATAG ACTGCTCTTG CATGTCAGGC
    ACTTTGCGTA GTAACATGGG AACCCTTGAA CAAGCCTTCC CAATTTCTCC TGTCTCTGTT
    AATGATTTCT CCACCAGTTT ACGTGGCTTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GCTAAGCCTG GCAAACTGCT GCCTTTGATT TCATTCAGTC AGGAAAAAAA CAGTGGGACA
    CACATACCAA TTATTACAGA GTTGGTGAAC GACTCAAATG TTCAGTTCTT GGACCAGGAT
    GATGATGATG ACCCAGATAC AGAGTTATAC TTGACTCAGC CCTTCGCCTG TGGGACCGCC
    TTTGCTGTCA GTGTGTTAGA CTCCCTTATG AGTGCAACAT ACTTCAATGA TAACATTCTG
    ACTCTAATCA GAACTCTGGT GACTGGGGGA GCAACACCAG AGCTGGAAGC CCTCATTGCA
    GAAGAGAACG CTCTGCGTGG GGGTTATAGC ACCCCACAAA CTCTAGCAAA CAGAGACCGC
    TGTCGAGTAG CCCAATTAGC CTTGTATGAT GGACCATTTG CAGATTTGGG GGATGGTGGC
    TGTTATGGAG ACCTTTACTG CAAAGCATTA AAAACTTACA ACATGCTGTG CTTTGGTATA
    TACCGACTCC GAGATGCCCA CCTCAGCACA CCCAGCCAGT GTACCAAAAG GTACGTTATC
    ACCAACCCTC CATATGAGTT TGAACTGGTT CCCACAGATC TCATCTTTTG CCTGATGCAG
    TTCGACCATA ATGCCAGCCA GTCCCGAGCT AGCCTGTCTC ACTCTTCACA CTCCTCGCAC
    TCATCTAGCA AAAAAAGTTC ATCTGTTACC TCCATTCTAC ACACAGCCTC AGCCAACCGT
    CAGAACAGAG TCAAGGCTCG AGATTCCCGT GACAAACAAA AGACGAATAG AATGGGCCAA
    GCAGAAAAGA AATGGTATAC AGATGAAACG GAAAACACTT ATCCCAGAAA CATTCAGATT
    AAGCCAATGA GCACACATAT GGCAAATCAG ATTAATCAAT ACAAATCAAC AAGCAGCTTG
    ATACCGCCAA TACGAGAGGT CGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761897.1
    CDS1059..4934
    Translation

    Target ORF information:

    RefSeq Version XM_031906037.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906037.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CCAAGATGTC CACCCGCAAG
    AGGCTGATAC AGGCATGTTG TATGGGGTGC TCTGAGATAG ACTGCTCTTG CATGTCAGGC
    ACTTTGCGTA GTAACATGGG AACCCTTGAA CAAGCCTTCC CAATTTCTCC TGTCTCTGTT
    AATGATTTCT CCACCAGTTT ACGTGGCTTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GCTAAGCCTG GCAAACTGCT GCCTTTGATT TCATTCAGTC AGGAAAAAAA CAGTGGGACA
    CACATACCAA TTATTACAGA GTTGGTGAAC GACTCAAATG TTCAGTTCTT GGACCAGGAT
    GATGATGATG ACCCAGATAC AGAGTTATAC TTGACTCAGC CCTTCGCCTG TGGGACCGCC
    TTTGCTGTCA GTGTGTTAGA CTCCCTTATG AGTGCAACAT ACTTCAATGA TAACATTCTG
    ACTCTAATCA GAACTCTGGT GACTGGGGGA GCAACACCAG AGCTGGAAGC CCTCATTGCA
    GAAGAGAACG CTCTGCGTGG GGGTTATAGC ACCCCACAAA CTCTAGCAAA CAGAGACCGC
    TGTCGAGTAG CCCAATTAGC CTTGTATGAT GGACCATTTG CAGATTTGGG GGATGGTGGC
    TGTTATGGAG ACCTTTACTG CAAAGCATTA AAAACTTACA ACATGCTGTG CTTTGGTATA
    TACCGACTCC GAGATGCCCA CCTCAGCACA CCCAGCCAGT GTACCAAAAG GTACGTTATC
    ACCAACCCTC CATATGAGTT TGAACTGGTT CCCACAGATC TCATCTTTTG CCTGATGCAG
    TTCGACCATA ATGCCAGCCA GTCCCGAGCT AGCCTGTCTC ACTCTTCACA CTCCTCGCAC
    TCATCTAGCA AAAAAAGTTC ATCTGTTACC TCCATTCTAC ACACAGCCTC AGCCAACCGT
    CAGAACAGAG TCAAGGCTCG AGATTCCCGT GACAAACAAA AGACGAATAG AATGGGCCAA
    GCAGAAAAGA AATGGTATAC AGATGAAACG GAAAACACTT ATCCCAGAAA CATTCAGATT
    AAGCCAATGA GCACACATAT GGCAAATCAG ATTAATCAAT ACAAATCAAC AAGCAGCTTG
    ATACCGCCAA TACGAGAGGT CGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62480
    Clone ID Related Accession (Same CDS sequence) XM_031906038.1
    Accession Version XM_031906038.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CTACAAGGGA AGAAAATAGA
    TTAAGTATCC ACAGGCTTTC TATTCATCCC CAGACAGCCA AAGCTAGCTA TTCTGTGGCC
    AGTTCCAAAC TCTGCACAGA ACAGCAGGAG CCTGTGCCTC TTGTTAATAA CCGCAAAGGA
    AGCCTGTTCT TGCCATGTGA TTCCTCTCTC CTGCACCTGC AGTTACTGGG TTCCTCTGGC
    ACTGAACACC GTACCAGTAT TAAATTGCAG CGTGCACTGA GCTTGCCAGG CAAATACCGC
    TACCACCCCA ATCAGCCAGT ACTCATCCAA AAGCAGTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG CTAAGCCTGG CAAACTGCTG CCTTTGATTT CATTCAGTCA GGAAAAAAAC
    AGTGGGACAC ACATACCAAT TATTACAGAG TTGGTGAACG ACTCAAATGT TCAGTTCTTG
    GACCAGGATG ATGATGATGA CCCAGATACA GAGTTATACT TGACTCAGCC CTTCGCCTGT
    GGGACCGCCT TTGCTGTCAG TGTGTTAGAC TCCCTTATGA GTGCAACATA CTTCAATGAT
    AACATTCTGA CTCTAATCAG AACTCTGGTG ACTGGGGGAG CAACACCAGA GCTGGAAGCC
    CTCATTGCAG AAGAGAACGC TCTGCGTGGG GGTTATAGCA CCCCACAAAC TCTAGCAAAC
    AGAGACCGCT GTCGAGTAGC CCAATTAGCC TTGTATGATG GACCATTTGC AGATTTGGGG
    GATGGTGGCT GTTATGGAGA CCTTTACTGC AAAGCATTAA AAACTTACAA CATGCTGTGC
    TTTGGTATAT ACCGACTCCG AGATGCCCAC CTCAGCACAC CCAGCCAGTG TACCAAAAGG
    TACGTTATCA CCAACCCTCC ATATGAGTTT GAACTGGTTC CCACAGATCT CATCTTTTGC
    CTGATGCAGT TCGACCATAA TGCCAGCCAG TCCCGAGCTA GCCTGTCTCA CTCTTCACAC
    TCCTCGCACT CATCTAGCAA AAAAAGTTCA TCTGTTACCT CCATTCTACA CACAGCCTCA
    GCCAACCGTC AGAACAGAGT CAAGGCTCGA GATTCCCGTG ACAAACAAAA CAGAAAAGAA
    ATGGTATACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761898.1
    CDS1062..4916
    Translation

    Target ORF information:

    RefSeq Version XM_031906038.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906038.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CTACAAGGGA AGAAAATAGA
    TTAAGTATCC ACAGGCTTTC TATTCATCCC CAGACAGCCA AAGCTAGCTA TTCTGTGGCC
    AGTTCCAAAC TCTGCACAGA ACAGCAGGAG CCTGTGCCTC TTGTTAATAA CCGCAAAGGA
    AGCCTGTTCT TGCCATGTGA TTCCTCTCTC CTGCACCTGC AGTTACTGGG TTCCTCTGGC
    ACTGAACACC GTACCAGTAT TAAATTGCAG CGTGCACTGA GCTTGCCAGG CAAATACCGC
    TACCACCCCA ATCAGCCAGT ACTCATCCAA AAGCAGTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG CTAAGCCTGG CAAACTGCTG CCTTTGATTT CATTCAGTCA GGAAAAAAAC
    AGTGGGACAC ACATACCAAT TATTACAGAG TTGGTGAACG ACTCAAATGT TCAGTTCTTG
    GACCAGGATG ATGATGATGA CCCAGATACA GAGTTATACT TGACTCAGCC CTTCGCCTGT
    GGGACCGCCT TTGCTGTCAG TGTGTTAGAC TCCCTTATGA GTGCAACATA CTTCAATGAT
    AACATTCTGA CTCTAATCAG AACTCTGGTG ACTGGGGGAG CAACACCAGA GCTGGAAGCC
    CTCATTGCAG AAGAGAACGC TCTGCGTGGG GGTTATAGCA CCCCACAAAC TCTAGCAAAC
    AGAGACCGCT GTCGAGTAGC CCAATTAGCC TTGTATGATG GACCATTTGC AGATTTGGGG
    GATGGTGGCT GTTATGGAGA CCTTTACTGC AAAGCATTAA AAACTTACAA CATGCTGTGC
    TTTGGTATAT ACCGACTCCG AGATGCCCAC CTCAGCACAC CCAGCCAGTG TACCAAAAGG
    TACGTTATCA CCAACCCTCC ATATGAGTTT GAACTGGTTC CCACAGATCT CATCTTTTGC
    CTGATGCAGT TCGACCATAA TGCCAGCCAG TCCCGAGCTA GCCTGTCTCA CTCTTCACAC
    TCCTCGCACT CATCTAGCAA AAAAAGTTCA TCTGTTACCT CCATTCTACA CACAGCCTCA
    GCCAACCGTC AGAACAGAGT CAAGGCTCGA GATTCCCGTG ACAAACAAAA CAGAAAAGAA
    ATGGTATACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62481
    Clone ID Related Accession (Same CDS sequence) XM_031906039.1
    Accession Version XM_031906039.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CTACAAGGGA AGAAAATAGA
    TTAAGTATCC ACAGGCTTTC TATTCATCCC CAGACAGCCA AAGCTAGCTA TTCTGTGGCC
    AGTTCCAAAC TCTGCACAGA ACAGCAGGAG CCTGTGCCTC TTGTTAATAA CCGCAAAGGA
    AGCCTGTTCT TGCCATGTGA TTCCTCTCTC CTGCACCTGC AGTTACTGGG TTCCTCTGGC
    ACTGAACACC GTACCAGTAT TAAATTGCAG CGTGCACTGA GCTTGCCAGG CAAATACCGC
    TACCACCCCA ATCAGCCAGT ACTCATCCAA AAGCAGTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG CTAAGCCTGG CAAACTGCTG CCTTTGATTT CATTCAGTCA GGAAAAAAAC
    AGTGGGACAC ACATACCAAT TATTACAGAG TTGGTGAACG ACTCAAATGT TCAGTTCTTG
    GACCAGGATG ATGATGATGA CCCAGATACA GAGTTATACT TGACTCAGCC CTTCGCCTGT
    GGGACCGCCT TTGCTGTCAG TGTGTTAGAC TCCCTTATGA GTGCAACATA CTTCAATGAT
    AACATTCTGA CTCTAATCAG AACTCTGGTG ACTGGGGGAG CAACACCAGA GCTGGAAGCC
    CTCATTGCAG AAGAGAACGC TCTGCGTGGG GGTTATAGCA CCCCACAAAC TCTAGCAAAC
    AGAGACCGCT GTCGAGTAGC CCAATTAGCC TTGTATGATG GACCATTTGC AGATTTGGGG
    GATGGTGGCT GTTATGGAGA CCTTTACTGC AAAGCATTAA AAACTTACAA CATGCTGTGC
    TTTGGTATAT ACCGACTCCG AGATGCCCAC CTCAGCACAC CCAGCCAGTG TACCAAAAGG
    TACGTTATCA CCAACCCTCC ATATGAGTTT GAACTGGTTC CCACAGATCT CATCTTTTGC
    CTGATGCAGT TCGACCATAA TGCCAGCCAG TCCCGAGCTA GCCTGTCTCA CTCTTCACAC
    TCCTCGCACT CATCTAGCAA AAAAAGTTCA TCTGTTACCT CCATTCTACA CACAGCCTCA
    GCCAACCGTC AGAACAGAGT CAAGGCTCGA GATTCCCGTG ACAAACAAAA AAAAGAAATG
    GTATACAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761899.1
    CDS1062..4913
    Translation

    Target ORF information:

    RefSeq Version XM_031906039.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906039.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CTACAAGGGA AGAAAATAGA
    TTAAGTATCC ACAGGCTTTC TATTCATCCC CAGACAGCCA AAGCTAGCTA TTCTGTGGCC
    AGTTCCAAAC TCTGCACAGA ACAGCAGGAG CCTGTGCCTC TTGTTAATAA CCGCAAAGGA
    AGCCTGTTCT TGCCATGTGA TTCCTCTCTC CTGCACCTGC AGTTACTGGG TTCCTCTGGC
    ACTGAACACC GTACCAGTAT TAAATTGCAG CGTGCACTGA GCTTGCCAGG CAAATACCGC
    TACCACCCCA ATCAGCCAGT ACTCATCCAA AAGCAGTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG CTAAGCCTGG CAAACTGCTG CCTTTGATTT CATTCAGTCA GGAAAAAAAC
    AGTGGGACAC ACATACCAAT TATTACAGAG TTGGTGAACG ACTCAAATGT TCAGTTCTTG
    GACCAGGATG ATGATGATGA CCCAGATACA GAGTTATACT TGACTCAGCC CTTCGCCTGT
    GGGACCGCCT TTGCTGTCAG TGTGTTAGAC TCCCTTATGA GTGCAACATA CTTCAATGAT
    AACATTCTGA CTCTAATCAG AACTCTGGTG ACTGGGGGAG CAACACCAGA GCTGGAAGCC
    CTCATTGCAG AAGAGAACGC TCTGCGTGGG GGTTATAGCA CCCCACAAAC TCTAGCAAAC
    AGAGACCGCT GTCGAGTAGC CCAATTAGCC TTGTATGATG GACCATTTGC AGATTTGGGG
    GATGGTGGCT GTTATGGAGA CCTTTACTGC AAAGCATTAA AAACTTACAA CATGCTGTGC
    TTTGGTATAT ACCGACTCCG AGATGCCCAC CTCAGCACAC CCAGCCAGTG TACCAAAAGG
    TACGTTATCA CCAACCCTCC ATATGAGTTT GAACTGGTTC CCACAGATCT CATCTTTTGC
    CTGATGCAGT TCGACCATAA TGCCAGCCAG TCCCGAGCTA GCCTGTCTCA CTCTTCACAC
    TCCTCGCACT CATCTAGCAA AAAAAGTTCA TCTGTTACCT CCATTCTACA CACAGCCTCA
    GCCAACCGTC AGAACAGAGT CAAGGCTCGA GATTCCCGTG ACAAACAAAA AAAAGAAATG
    GTATACAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62482
    Clone ID Related Accession (Same CDS sequence) XM_031906040.1
    Accession Version XM_031906040.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TTATTTATTC CAAGATGTCC
    ACCCGCAAGA GGCTGATACA GGCATGTTGT ATGGGGTGCT CTGAGATAGA CTGCTCTTGC
    ATGTCAGGCA CTTTGCGTAG TAACATGGGA ACCCTTGAAC AAGCCTTCCC AATTTCTCCT
    GTCTCTGTTA ATGATTTCTC CACCAGTTTA CGTGGCTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG TGAACGACTC AAATGTTCAG TTCTTGGACC AGGATGATGA TGATGACCCA
    GATACAGAGT TATACTTGAC TCAGCCCTTC GCCTGTGGGA CCGCCTTTGC TGTCAGTGTG
    TTAGACTCCC TTATGAGTGC AACATACTTC AATGATAACA TTCTGACTCT AATCAGAACT
    CTGGTGACTG GGGGAGCAAC ACCAGAGCTG GAAGCCCTCA TTGCAGAAGA GAACGCTCTG
    CGTGGGGGTT ATAGCACCCC ACAAACTCTA GCAAACAGAG ACCGCTGTCG AGTAGCCCAA
    TTAGCCTTGT ATGATGGACC ATTTGCAGAT TTGGGGGATG GTGGCTGTTA TGGAGACCTT
    TACTGCAAAG CATTAAAAAC TTACAACATG CTGTGCTTTG GTATATACCG ACTCCGAGAT
    GCCCACCTCA GCACACCCAG CCAGTGTACC AAAAGGTACG TTATCACCAA CCCTCCATAT
    GAGTTTGAAC TGGTTCCCAC AGATCTCATC TTTTGCCTGA TGCAGTTCGA CCATAATGCC
    AGCCAGTCCC GAGCTAGCCT GTCTCACTCT TCACACTCCT CGCACTCATC TAGCAAAAAA
    AGTTCATCTG TTACCTCCAT TCTACACACA GCCTCAGCCA ACCGTCAGAA CAGAGTCAAG
    GCTCGAGATT CCCGTGACAA ACAAAAGACG AATAGAATGG GCCAAGCAGA AAAGAAATGG
    TATACAGATG AAACGGAAAA CACTTATCCC AGAAACATTC AGATTAAGCC AATGAGCACA
    CATATGGCAA ATCAGATTAA TCAATACAAA TCAACAAGCA GCTTGATACC GCCAATACGA
    GAGGTCGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761900.1
    CDS1054..4854
    Translation

    Target ORF information:

    RefSeq Version XM_031906040.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906040.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TTATTTATTC CAAGATGTCC
    ACCCGCAAGA GGCTGATACA GGCATGTTGT ATGGGGTGCT CTGAGATAGA CTGCTCTTGC
    ATGTCAGGCA CTTTGCGTAG TAACATGGGA ACCCTTGAAC AAGCCTTCCC AATTTCTCCT
    GTCTCTGTTA ATGATTTCTC CACCAGTTTA CGTGGCTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG TGAACGACTC AAATGTTCAG TTCTTGGACC AGGATGATGA TGATGACCCA
    GATACAGAGT TATACTTGAC TCAGCCCTTC GCCTGTGGGA CCGCCTTTGC TGTCAGTGTG
    TTAGACTCCC TTATGAGTGC AACATACTTC AATGATAACA TTCTGACTCT AATCAGAACT
    CTGGTGACTG GGGGAGCAAC ACCAGAGCTG GAAGCCCTCA TTGCAGAAGA GAACGCTCTG
    CGTGGGGGTT ATAGCACCCC ACAAACTCTA GCAAACAGAG ACCGCTGTCG AGTAGCCCAA
    TTAGCCTTGT ATGATGGACC ATTTGCAGAT TTGGGGGATG GTGGCTGTTA TGGAGACCTT
    TACTGCAAAG CATTAAAAAC TTACAACATG CTGTGCTTTG GTATATACCG ACTCCGAGAT
    GCCCACCTCA GCACACCCAG CCAGTGTACC AAAAGGTACG TTATCACCAA CCCTCCATAT
    GAGTTTGAAC TGGTTCCCAC AGATCTCATC TTTTGCCTGA TGCAGTTCGA CCATAATGCC
    AGCCAGTCCC GAGCTAGCCT GTCTCACTCT TCACACTCCT CGCACTCATC TAGCAAAAAA
    AGTTCATCTG TTACCTCCAT TCTACACACA GCCTCAGCCA ACCGTCAGAA CAGAGTCAAG
    GCTCGAGATT CCCGTGACAA ACAAAAGACG AATAGAATGG GCCAAGCAGA AAAGAAATGG
    TATACAGATG AAACGGAAAA CACTTATCCC AGAAACATTC AGATTAAGCC AATGAGCACA
    CATATGGCAA ATCAGATTAA TCAATACAAA TCAACAAGCA GCTTGATACC GCCAATACGA
    GAGGTCGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62483
    Clone ID Related Accession (Same CDS sequence) XM_031906041.1
    Accession Version XM_031906041.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CCAAGATGTC CACCCGCAAG
    AGGCTGATAC AGGCATGTTG TATGGGGTGC TCTGAGATAG ACTGCTCTTG CATGTCAGGC
    ACTTTGCGTA GTAACATGGG AACCCTTGAA CAAGCCTTCC CAATTTCTCC TGTCTCTGTT
    AATGATTTCT CCACCAGTTT ACGTGGCTTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GTGAACGACT CAAATGTTCA GTTCTTGGAC CAGGATGATG ATGATGACCC AGATACAGAG
    TTATACTTGA CTCAGCCCTT CGCCTGTGGG ACCGCCTTTG CTGTCAGTGT GTTAGACTCC
    CTTATGAGTG CAACATACTT CAATGATAAC ATTCTGACTC TAATCAGAAC TCTGGTGACT
    GGGGGAGCAA CACCAGAGCT GGAAGCCCTC ATTGCAGAAG AGAACGCTCT GCGTGGGGGT
    TATAGCACCC CACAAACTCT AGCAAACAGA GACCGCTGTC GAGTAGCCCA ATTAGCCTTG
    TATGATGGAC CATTTGCAGA TTTGGGGGAT GGTGGCTGTT ATGGAGACCT TTACTGCAAA
    GCATTAAAAA CTTACAACAT GCTGTGCTTT GGTATATACC GACTCCGAGA TGCCCACCTC
    AGCACACCCA GCCAGTGTAC CAAAAGGTAC GTTATCACCA ACCCTCCATA TGAGTTTGAA
    CTGGTTCCCA CAGATCTCAT CTTTTGCCTG ATGCAGTTCG ACCATAATGC CAGCCAGTCC
    CGAGCTAGCC TGTCTCACTC TTCACACTCC TCGCACTCAT CTAGCAAAAA AAGTTCATCT
    GTTACCTCCA TTCTACACAC AGCCTCAGCC AACCGTCAGA ACAGAGTCAA GGCTCGAGAT
    TCCCGTGACA AACAAAAGAC GAATAGAATG GGCCAAGCAG AAAAGAAATG GTATACAGAT
    GAAACGGAAA ACACTTATCC CAGAAACATT CAGATTAAGC CAATGAGCAC ACATATGGCA
    AATCAGATTA ATCAATACAA ATCAACAAGC AGCTTGATAC CGCCAATACG AGAGGTCGAA
    GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761901.1
    CDS1053..4844
    Translation

    Target ORF information:

    RefSeq Version XM_031906041.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC CCAAGATGTC CACCCGCAAG
    AGGCTGATAC AGGCATGTTG TATGGGGTGC TCTGAGATAG ACTGCTCTTG CATGTCAGGC
    ACTTTGCGTA GTAACATGGG AACCCTTGAA CAAGCCTTCC CAATTTCTCC TGTCTCTGTT
    AATGATTTCT CCACCAGTTT ACGTGGCTTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GTGAACGACT CAAATGTTCA GTTCTTGGAC CAGGATGATG ATGATGACCC AGATACAGAG
    TTATACTTGA CTCAGCCCTT CGCCTGTGGG ACCGCCTTTG CTGTCAGTGT GTTAGACTCC
    CTTATGAGTG CAACATACTT CAATGATAAC ATTCTGACTC TAATCAGAAC TCTGGTGACT
    GGGGGAGCAA CACCAGAGCT GGAAGCCCTC ATTGCAGAAG AGAACGCTCT GCGTGGGGGT
    TATAGCACCC CACAAACTCT AGCAAACAGA GACCGCTGTC GAGTAGCCCA ATTAGCCTTG
    TATGATGGAC CATTTGCAGA TTTGGGGGAT GGTGGCTGTT ATGGAGACCT TTACTGCAAA
    GCATTAAAAA CTTACAACAT GCTGTGCTTT GGTATATACC GACTCCGAGA TGCCCACCTC
    AGCACACCCA GCCAGTGTAC CAAAAGGTAC GTTATCACCA ACCCTCCATA TGAGTTTGAA
    CTGGTTCCCA CAGATCTCAT CTTTTGCCTG ATGCAGTTCG ACCATAATGC CAGCCAGTCC
    CGAGCTAGCC TGTCTCACTC TTCACACTCC TCGCACTCAT CTAGCAAAAA AAGTTCATCT
    GTTACCTCCA TTCTACACAC AGCCTCAGCC AACCGTCAGA ACAGAGTCAA GGCTCGAGAT
    TCCCGTGACA AACAAAAGAC GAATAGAATG GGCCAAGCAG AAAAGAAATG GTATACAGAT
    GAAACGGAAA ACACTTATCC CAGAAACATT CAGATTAAGC CAATGAGCAC ACATATGGCA
    AATCAGATTA ATCAATACAA ATCAACAAGC AGCTTGATAC CGCCAATACG AGAGGTCGAA
    GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62484
    Clone ID Related Accession (Same CDS sequence) XM_031906042.1
    Accession Version XM_031906042.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TCCTCCTCAC TCTTCCTTGC
    CTGACCGCTT TGCCTGTGTT TGCAGTTGAA GATGAGCAAC CATCAGCCTT GTCACCAAAA
    AAAAAGCAAC GAAATGGAGG AATGAGGCAA TCTCCAAACT CTTCACCTAA CATGATGAGG
    CATGATCCCC TTCTTATGAC TGGAAATGAT CAAATTGATA ATATGGATTC TAGCAGTGTT
    AAAAGATATG ATTCTACTGG TATGTTCCAC TGGTGTCCAG CCAAGGAATT GGATAAAGTG
    CTTCTGACAC GGAGTGAAGC TGCCATGACA GTCCTCAGTG GGCATGTGGT TGTCTGCATT
    TTTGGAGACA TGACATCGGC ACTGATTGGA GTTCGGAATT TGGTGATGCC ACTGAGAGCC
    AGCAATTTTC ATTACCATGA GTTGAAACAT ATAGTCTTTG TTGGGTCCCT TGATTACCTT
    AAAAGAGAAT GGGAAACACT ACACAACTTC CCAAAGGTTT CCATCTTGCC TGGCACACCA
    TTAAGTCGAG CAGATCTGAG AGCTGTCAAC ATTAACCTGT GTGACATGTG CGTTATTCTG
    TCAGCCAATC AGAATAATAT TGATGATACA TCACTGCAGG ACAAAGAATG CATTTTGGCA
    TCTCTCAACA TCAAATCCAT GCAGTTTGAT GACAGCATAG GGCTCTTGCA AGCTAACTCT
    CAAGGGTTTA CGCCCCCAGG AATGGAGAGA TCCTCACCTG ATAATAGCCC AGTGCATGGT
    GTTGCAAGAC AGGCATCCAT AACTACAGGG GCCAACATTC CCATAATTAC AGAACTTGCT
    AAGCCTGGCA AACTGCTGCC TTTGATTTCA TTCAGTCAGG AAAAAAACAG TGGGACACAC
    ATACCAATTA TTACAGAGTT GGTGAACGAC TCAAATGTTC AGTTCTTGGA CCAGGATGAT
    GATGATGACC CAGATACAGA GTTATACTTG ACTCAGCCCT TCGCCTGTGG GACCGCCTTT
    GCTGTCAGTG TGTTAGACTC CCTTATGAGT GCAACATACT TCAATGATAA CATTCTGACT
    CTAATCAGAA CTCTGGTGAC TGGGGGAGCA ACACCAGAGC TGGAAGCCCT CATTGCAGAA
    GAGAACGCTC TGCGTGGGGG TTATAGCACC CCACAAACTC TAGCAAACAG AGACCGCTGT
    CGAGTAGCCC AATTAGCCTT GTATGATGGA CCATTTGCAG ATTTGGGGGA TGGTGGCTGT
    TATGGAGACC TTTACTGCAA AGCATTAAAA ACTTACAACA TGCTGTGCTT TGGTATATAC
    CGACTCCGAG ATGCCCACCT CAGCACACCC AGCCAGTGTA CCAAAAGGTA CGTTATCACC
    AACCCTCCAT ATGAGTTTGA ACTGGTTCCC ACAGATCTCA TCTTTTGCCT GATGCAGTTC
    GACCATAATG CCAGCCAGTC CCGAGCTAGC CTGTCTCACT CTTCACACTC CTCGCACTCA
    TCTAGCAAAA AAAGTTCATC TGTTACCTCC ATTCTACACA CAGCCTCAGC CAACCGTCAG
    AACAGAGTCA AGGCTCGAGA TTCCCGTGAC AAACAAAAGA CGAATAGAAT GGGCCAAGCA
    GAAAAGAAAT GGTATACAGA TGAAACGGAA AACACTTATC CCAGAAACAT TCAGATTAAG
    CCAATGAGCA CACATATGGC AAATCAGATT AATCAATACA AATCAACAAG CAGCTTGATA
    CCGCCAATAC GAGAGGTCGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761902.1
    CDS1055..4807
    Translation

    Target ORF information:

    RefSeq Version XM_031906042.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906042.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TCCTCCTCAC TCTTCCTTGC
    CTGACCGCTT TGCCTGTGTT TGCAGTTGAA GATGAGCAAC CATCAGCCTT GTCACCAAAA
    AAAAAGCAAC GAAATGGAGG AATGAGGCAA TCTCCAAACT CTTCACCTAA CATGATGAGG
    CATGATCCCC TTCTTATGAC TGGAAATGAT CAAATTGATA ATATGGATTC TAGCAGTGTT
    AAAAGATATG ATTCTACTGG TATGTTCCAC TGGTGTCCAG CCAAGGAATT GGATAAAGTG
    CTTCTGACAC GGAGTGAAGC TGCCATGACA GTCCTCAGTG GGCATGTGGT TGTCTGCATT
    TTTGGAGACA TGACATCGGC ACTGATTGGA GTTCGGAATT TGGTGATGCC ACTGAGAGCC
    AGCAATTTTC ATTACCATGA GTTGAAACAT ATAGTCTTTG TTGGGTCCCT TGATTACCTT
    AAAAGAGAAT GGGAAACACT ACACAACTTC CCAAAGGTTT CCATCTTGCC TGGCACACCA
    TTAAGTCGAG CAGATCTGAG AGCTGTCAAC ATTAACCTGT GTGACATGTG CGTTATTCTG
    TCAGCCAATC AGAATAATAT TGATGATACA TCACTGCAGG ACAAAGAATG CATTTTGGCA
    TCTCTCAACA TCAAATCCAT GCAGTTTGAT GACAGCATAG GGCTCTTGCA AGCTAACTCT
    CAAGGGTTTA CGCCCCCAGG AATGGAGAGA TCCTCACCTG ATAATAGCCC AGTGCATGGT
    GTTGCAAGAC AGGCATCCAT AACTACAGGG GCCAACATTC CCATAATTAC AGAACTTGCT
    AAGCCTGGCA AACTGCTGCC TTTGATTTCA TTCAGTCAGG AAAAAAACAG TGGGACACAC
    ATACCAATTA TTACAGAGTT GGTGAACGAC TCAAATGTTC AGTTCTTGGA CCAGGATGAT
    GATGATGACC CAGATACAGA GTTATACTTG ACTCAGCCCT TCGCCTGTGG GACCGCCTTT
    GCTGTCAGTG TGTTAGACTC CCTTATGAGT GCAACATACT TCAATGATAA CATTCTGACT
    CTAATCAGAA CTCTGGTGAC TGGGGGAGCA ACACCAGAGC TGGAAGCCCT CATTGCAGAA
    GAGAACGCTC TGCGTGGGGG TTATAGCACC CCACAAACTC TAGCAAACAG AGACCGCTGT
    CGAGTAGCCC AATTAGCCTT GTATGATGGA CCATTTGCAG ATTTGGGGGA TGGTGGCTGT
    TATGGAGACC TTTACTGCAA AGCATTAAAA ACTTACAACA TGCTGTGCTT TGGTATATAC
    CGACTCCGAG ATGCCCACCT CAGCACACCC AGCCAGTGTA CCAAAAGGTA CGTTATCACC
    AACCCTCCAT ATGAGTTTGA ACTGGTTCCC ACAGATCTCA TCTTTTGCCT GATGCAGTTC
    GACCATAATG CCAGCCAGTC CCGAGCTAGC CTGTCTCACT CTTCACACTC CTCGCACTCA
    TCTAGCAAAA AAAGTTCATC TGTTACCTCC ATTCTACACA CAGCCTCAGC CAACCGTCAG
    AACAGAGTCA AGGCTCGAGA TTCCCGTGAC AAACAAAAGA CGAATAGAAT GGGCCAAGCA
    GAAAAGAAAT GGTATACAGA TGAAACGGAA AACACTTATC CCAGAAACAT TCAGATTAAG
    CCAATGAGCA CACATATGGC AAATCAGATT AATCAATACA AATCAACAAG CAGCTTGATA
    CCGCCAATAC GAGAGGTCGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62485
    Clone ID Related Accession (Same CDS sequence) XM_031906043.1
    Accession Version XM_031906043.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TTATTTATTT TGAAGATGAG
    CAACCATCAG CCTTGTCACC AAAAAAAAAG CAACGAAATG GAGGAATGAG GCAATCTCCA
    AACTCTTCAC CTAACATGAT GAGGCATGAT CCCCTTCTTA TGACTGGAAA TGATCAAATT
    GATAATATGG ATTCTAGCAG TGTTAAAAGA TATGATTCTA CTGGTATGTT CCACTGGTGT
    CCAGCCAAGG AATTGGATAA AGTGCTTCTG ACACGGAGTG AAGCTGCCAT GACAGTCCTC
    AGTGGGCATG TGGTTGTCTG CATTTTTGGA GACATGACAT CGGCACTGAT TGGAGTTCGG
    AATTTGGTGA TGCCACTGAG AGCCAGCAAT TTTCATTACC ATGAGTTGAA ACATATAGTC
    TTTGTTGGGT CCCTTGATTA CCTTAAAAGA GAATGGGAAA CACTACACAA CTTCCCAAAG
    GTTTCCATCT TGCCTGGCAC ACCATTAAGT CGAGCAGATC TGAGAGCTGT CAACATTAAC
    CTGTGTGACA TGTGCGTTAT TCTGTCAGCC AATCAGAATA ATATTGATGA TACATCACTG
    CAGGACAAAG AATGCATTTT GGCATCTCTC AACATCAAAT CCATGCAGTT TGATGACAGC
    ATAGGGCTCT TGCAAGCTAA CTCTCAAGGG TTTACGCCCC CAGGAATGGA GAGATCCTCA
    CCTGATAATA GCCCAGTGCA TGGTGTTGCA AGACAGGCAT CCATAACTAC AGGGGCCAAC
    ATTCCCATAA TTACAGAACT TGCTAAGCCT GGCAAACTGC TGCCTTTGAT TTCATTCAGT
    CAGGAAAAAA ACAGTGGGAC ACACATACCA ATTATTACAG AGTTGGTGAA CGACTCAAAT
    GTTCAGTTCT TGGACCAGGA TGATGATGAT GACCCAGATA CAGAGTTATA CTTGACTCAG
    CCCTTCGCCT GTGGGACCGC CTTTGCTGTC AGTGTGTTAG ACTCCCTTAT GAGTGCAACA
    TACTTCAATG ATAACATTCT GACTCTAATC AGAACTCTGG TGACTGGGGG AGCAACACCA
    GAGCTGGAAG CCCTCATTGC AGAAGAGAAC GCTCTGCGTG GGGGTTATAG CACCCCACAA
    ACTCTAGCAA ACAGAGACCG CTGTCGAGTA GCCCAATTAG CCTTGTATGA TGGACCATTT
    GCAGATTTGG GGGATGGTGG CTGTTATGGA GACCTTTACT GCAAAGCATT AAAAACTTAC
    AACATGCTGT GCTTTGGTAT ATACCGACTC CGAGATGCCC ACCTCAGCAC ACCCAGCCAG
    TGTACCAAAA GGTACGTTAT CACCAACCCT CCATATGAGT TTGAACTGGT TCCCACAGAT
    CTCATCTTTT GCCTGATGCA GTTCGACCAT AATGCCAGCC AGTCCCGAGC TAGCCTGTCT
    CACTCTTCAC ACTCCTCGCA CTCATCTAGC AAAAAAAGTT CATCTGTTAC CTCCATTCTA
    CACACAGCCT CAGCCAACCG TCAGAACAGA GTCAAGGCTC GAGATTCCCG TGACAAACAA
    AAGACGAATA GAATGGGCCA AGCAGAAAAG AAATGGTATA CAGATGAAAC GGAAAACACT
    TATCCCAGAA ACATTCAGAT TAAGCCAATG AGCACACATA TGGCAAATCA GATTAATCAA
    TACAAATCAA CAAGCAGCTT GATACCGCCA ATACGAGAGG TCGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761903.1
    CDS1054..4770
    Translation

    Target ORF information:

    RefSeq Version XM_031906043.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906043.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TTATTTATTT TGAAGATGAG
    CAACCATCAG CCTTGTCACC AAAAAAAAAG CAACGAAATG GAGGAATGAG GCAATCTCCA
    AACTCTTCAC CTAACATGAT GAGGCATGAT CCCCTTCTTA TGACTGGAAA TGATCAAATT
    GATAATATGG ATTCTAGCAG TGTTAAAAGA TATGATTCTA CTGGTATGTT CCACTGGTGT
    CCAGCCAAGG AATTGGATAA AGTGCTTCTG ACACGGAGTG AAGCTGCCAT GACAGTCCTC
    AGTGGGCATG TGGTTGTCTG CATTTTTGGA GACATGACAT CGGCACTGAT TGGAGTTCGG
    AATTTGGTGA TGCCACTGAG AGCCAGCAAT TTTCATTACC ATGAGTTGAA ACATATAGTC
    TTTGTTGGGT CCCTTGATTA CCTTAAAAGA GAATGGGAAA CACTACACAA CTTCCCAAAG
    GTTTCCATCT TGCCTGGCAC ACCATTAAGT CGAGCAGATC TGAGAGCTGT CAACATTAAC
    CTGTGTGACA TGTGCGTTAT TCTGTCAGCC AATCAGAATA ATATTGATGA TACATCACTG
    CAGGACAAAG AATGCATTTT GGCATCTCTC AACATCAAAT CCATGCAGTT TGATGACAGC
    ATAGGGCTCT TGCAAGCTAA CTCTCAAGGG TTTACGCCCC CAGGAATGGA GAGATCCTCA
    CCTGATAATA GCCCAGTGCA TGGTGTTGCA AGACAGGCAT CCATAACTAC AGGGGCCAAC
    ATTCCCATAA TTACAGAACT TGCTAAGCCT GGCAAACTGC TGCCTTTGAT TTCATTCAGT
    CAGGAAAAAA ACAGTGGGAC ACACATACCA ATTATTACAG AGTTGGTGAA CGACTCAAAT
    GTTCAGTTCT TGGACCAGGA TGATGATGAT GACCCAGATA CAGAGTTATA CTTGACTCAG
    CCCTTCGCCT GTGGGACCGC CTTTGCTGTC AGTGTGTTAG ACTCCCTTAT GAGTGCAACA
    TACTTCAATG ATAACATTCT GACTCTAATC AGAACTCTGG TGACTGGGGG AGCAACACCA
    GAGCTGGAAG CCCTCATTGC AGAAGAGAAC GCTCTGCGTG GGGGTTATAG CACCCCACAA
    ACTCTAGCAA ACAGAGACCG CTGTCGAGTA GCCCAATTAG CCTTGTATGA TGGACCATTT
    GCAGATTTGG GGGATGGTGG CTGTTATGGA GACCTTTACT GCAAAGCATT AAAAACTTAC
    AACATGCTGT GCTTTGGTAT ATACCGACTC CGAGATGCCC ACCTCAGCAC ACCCAGCCAG
    TGTACCAAAA GGTACGTTAT CACCAACCCT CCATATGAGT TTGAACTGGT TCCCACAGAT
    CTCATCTTTT GCCTGATGCA GTTCGACCAT AATGCCAGCC AGTCCCGAGC TAGCCTGTCT
    CACTCTTCAC ACTCCTCGCA CTCATCTAGC AAAAAAAGTT CATCTGTTAC CTCCATTCTA
    CACACAGCCT CAGCCAACCG TCAGAACAGA GTCAAGGCTC GAGATTCCCG TGACAAACAA
    AAGACGAATA GAATGGGCCA AGCAGAAAAG AAATGGTATA CAGATGAAAC GGAAAACACT
    TATCCCAGAA ACATTCAGAT TAAGCCAATG AGCACACATA TGGCAAATCA GATTAATCAA
    TACAAATCAA CAAGCAGCTT GATACCGCCA ATACGAGAGG TCGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62486
    Clone ID Related Accession (Same CDS sequence) XM_031906044.1
    Accession Version XM_031906044.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TTGAAGATGA GCAACCATCA
    GCCTTGTCAC CAAAAAAAAA GCAACGAAAT GGAGGAATGA GGCAATCTCC AAACTCTTCA
    CCTAACATGA TGAGGCATGA TCCCCTTCTT ATGACTGGAA ATGATCAAAT TGATAATATG
    GATTCTAGCA GTGTTAAAAG ATATGATTCT ACTGGTATGT TCCACTGGTG TCCAGCCAAG
    GAATTGGATA AAGTGCTTCT GACACGGAGT GAAGCTGCCA TGACAGTCCT CAGTGGGCAT
    GTGGTTGTCT GCATTTTTGG AGACATGACA TCGGCACTGA TTGGAGTTCG GAATTTGGTG
    ATGCCACTGA GAGCCAGCAA TTTTCATTAC CATGAGTTGA AACATATAGT CTTTGTTGGG
    TCCCTTGATT ACCTTAAAAG AGAATGGGAA ACACTACACA ACTTCCCAAA GGTTTCCATC
    TTGCCTGGCA CACCATTAAG TCGAGCAGAT CTGAGAGCTG TCAACATTAA CCTGTGTGAC
    ATGTGCGTTA TTCTGTCAGC CAATCAGAAT AATATTGATG ATACATCACT GCAGGACAAA
    GAATGCATTT TGGCATCTCT CAACATCAAA TCCATGCAGT TTGATGACAG CATAGGGCTC
    TTGCAAGCTA ACTCTCAAGG GTTTACGCCC CCAGGAATGG AGAGATCCTC ACCTGATAAT
    AGCCCAGTGC ATGGTGTTGC AAGACAGGCA TCCATAACTA CAGGGGCCAA CATTCCCATA
    ATTACAGAAC TTGCTAAGCC TGGCAAACTG CTGCCTTTGA TTTCATTCAG TCAGGAAAAA
    AACAGTGGGA CACACATACC AATTATTACA GAGTTGGTGA ACGACTCAAA TGTTCAGTTC
    TTGGACCAGG ATGATGATGA TGACCCAGAT ACAGAGTTAT ACTTGACTCA GCCCTTCGCC
    TGTGGGACCG CCTTTGCTGT CAGTGTGTTA GACTCCCTTA TGAGTGCAAC ATACTTCAAT
    GATAACATTC TGACTCTAAT CAGAACTCTG GTGACTGGGG GAGCAACACC AGAGCTGGAA
    GCCCTCATTG CAGAAGAGAA CGCTCTGCGT GGGGGTTATA GCACCCCACA AACTCTAGCA
    AACAGAGACC GCTGTCGAGT AGCCCAATTA GCCTTGTATG ATGGACCATT TGCAGATTTG
    GGGGATGGTG GCTGTTATGG AGACCTTTAC TGCAAAGCAT TAAAAACTTA CAACATGCTG
    TGCTTTGGTA TATACCGACT CCGAGATGCC CACCTCAGCA CACCCAGCCA GTGTACCAAA
    AGGTACGTTA TCACCAACCC TCCATATGAG TTTGAACTGG TTCCCACAGA TCTCATCTTT
    TGCCTGATGC AGTTCGACCA TAATGCCAGC CAGTCCCGAG CTAGCCTGTC TCACTCTTCA
    CACTCCTCGC ACTCATCTAG CAAAAAAAGT TCATCTGTTA CCTCCATTCT ACACACAGCC
    TCAGCCAACC GTCAGAACAG AGTCAAGGCT CGAGATTCCC GTGACAAACA AAAGACGAAT
    AGAATGGGCC AAGCAGAAAA GAAATGGTAT ACAGATGAAA CGGAAAACAC TTATCCCAGA
    AACATTCAGA TTAAGCCAAT GAGCACACAT ATGGCAAATC AGATTAATCA ATACAAATCA
    ACAAGCAGCT TGATACCGCC AATACGAGAG GTCGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761904.1
    CDS1053..4760
    Translation

    Target ORF information:

    RefSeq Version XM_031906044.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906044.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TTGAAGATGA GCAACCATCA
    GCCTTGTCAC CAAAAAAAAA GCAACGAAAT GGAGGAATGA GGCAATCTCC AAACTCTTCA
    CCTAACATGA TGAGGCATGA TCCCCTTCTT ATGACTGGAA ATGATCAAAT TGATAATATG
    GATTCTAGCA GTGTTAAAAG ATATGATTCT ACTGGTATGT TCCACTGGTG TCCAGCCAAG
    GAATTGGATA AAGTGCTTCT GACACGGAGT GAAGCTGCCA TGACAGTCCT CAGTGGGCAT
    GTGGTTGTCT GCATTTTTGG AGACATGACA TCGGCACTGA TTGGAGTTCG GAATTTGGTG
    ATGCCACTGA GAGCCAGCAA TTTTCATTAC CATGAGTTGA AACATATAGT CTTTGTTGGG
    TCCCTTGATT ACCTTAAAAG AGAATGGGAA ACACTACACA ACTTCCCAAA GGTTTCCATC
    TTGCCTGGCA CACCATTAAG TCGAGCAGAT CTGAGAGCTG TCAACATTAA CCTGTGTGAC
    ATGTGCGTTA TTCTGTCAGC CAATCAGAAT AATATTGATG ATACATCACT GCAGGACAAA
    GAATGCATTT TGGCATCTCT CAACATCAAA TCCATGCAGT TTGATGACAG CATAGGGCTC
    TTGCAAGCTA ACTCTCAAGG GTTTACGCCC CCAGGAATGG AGAGATCCTC ACCTGATAAT
    AGCCCAGTGC ATGGTGTTGC AAGACAGGCA TCCATAACTA CAGGGGCCAA CATTCCCATA
    ATTACAGAAC TTGCTAAGCC TGGCAAACTG CTGCCTTTGA TTTCATTCAG TCAGGAAAAA
    AACAGTGGGA CACACATACC AATTATTACA GAGTTGGTGA ACGACTCAAA TGTTCAGTTC
    TTGGACCAGG ATGATGATGA TGACCCAGAT ACAGAGTTAT ACTTGACTCA GCCCTTCGCC
    TGTGGGACCG CCTTTGCTGT CAGTGTGTTA GACTCCCTTA TGAGTGCAAC ATACTTCAAT
    GATAACATTC TGACTCTAAT CAGAACTCTG GTGACTGGGG GAGCAACACC AGAGCTGGAA
    GCCCTCATTG CAGAAGAGAA CGCTCTGCGT GGGGGTTATA GCACCCCACA AACTCTAGCA
    AACAGAGACC GCTGTCGAGT AGCCCAATTA GCCTTGTATG ATGGACCATT TGCAGATTTG
    GGGGATGGTG GCTGTTATGG AGACCTTTAC TGCAAAGCAT TAAAAACTTA CAACATGCTG
    TGCTTTGGTA TATACCGACT CCGAGATGCC CACCTCAGCA CACCCAGCCA GTGTACCAAA
    AGGTACGTTA TCACCAACCC TCCATATGAG TTTGAACTGG TTCCCACAGA TCTCATCTTT
    TGCCTGATGC AGTTCGACCA TAATGCCAGC CAGTCCCGAG CTAGCCTGTC TCACTCTTCA
    CACTCCTCGC ACTCATCTAG CAAAAAAAGT TCATCTGTTA CCTCCATTCT ACACACAGCC
    TCAGCCAACC GTCAGAACAG AGTCAAGGCT CGAGATTCCC GTGACAAACA AAAGACGAAT
    AGAATGGGCC AAGCAGAAAA GAAATGGTAT ACAGATGAAA CGGAAAACAC TTATCCCAGA
    AACATTCAGA TTAAGCCAAT GAGCACACAT ATGGCAAATC AGATTAATCA ATACAAATCA
    ACAAGCAGCT TGATACCGCC AATACGAGAG GTCGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62487
    Clone ID Related Accession (Same CDS sequence) XM_031906045.1
    Accession Version XM_031906045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TTGAAGATGA GCAACCATCA
    GCCTTGTCAC CAAAAAAAAA GCAACGAAAT GGAGGAATGA GGCAATCTCC AAACTCTTCA
    CCTAACATGA TGAGGCATGA TCCCCTTCTT ATGACTGGAA ATGATCAAAT TGATAATATG
    GATTCTAGCA GTGTTAAAAG ATATGATTCT ACTGGTATGT TCCACTGGTG TCCAGCCAAG
    GAATTGGATA AAGTGCTTCT GACACGGAGT GAAGCTGCCA TGACAGTCCT CAGTGGGCAT
    GTGGTTGTCT GCATTTTTGG AGACATGACA TCGGCACTGA TTGGAGTTCG GAATTTGGTG
    ATGCCACTGA GAGCCAGCAA TTTTCATTAC CATGAGTTGA AACATATAGT CTTTGTTGGG
    TCCCTTGATT ACCTTAAAAG AGAATGGGAA ACACTACACA ACTTCCCAAA GGTTTCCATC
    TTGCCTGGCA CACCATTAAG TCGAGCAGAT CTGAGAGCTG TCAACATTAA CCTGTGTGAC
    ATGTGCGTTA TTCTGTCAGC CAATCAGAAT AATATTGATG ATACATCACT GCAGGACAAA
    GAATGCATTT TGGCATCTCT CAACATCAAA TCCATGCAGT TTGATGACAG CATAGGGCTC
    TTGCAAGCTA ACTCTCAAGG GTTTACGCCC CCAGGAATGG AGAGATCCTC ACCTGATAAT
    AGCCCAGTGC ATGGTGTTGC AAGACAGGCA TCCATAACTA CAGGGGCCAA CATTCCCATA
    ATTACAGAAC TTGTGAACGA CTCAAATGTT CAGTTCTTGG ACCAGGATGA TGATGATGAC
    CCAGATACAG AGTTATACTT GACTCAGCCC TTCGCCTGTG GGACCGCCTT TGCTGTCAGT
    GTGTTAGACT CCCTTATGAG TGCAACATAC TTCAATGATA ACATTCTGAC TCTAATCAGA
    ACTCTGGTGA CTGGGGGAGC AACACCAGAG CTGGAAGCCC TCATTGCAGA AGAGAACGCT
    CTGCGTGGGG GTTATAGCAC CCCACAAACT CTAGCAAACA GAGACCGCTG TCGAGTAGCC
    CAATTAGCCT TGTATGATGG ACCATTTGCA GATTTGGGGG ATGGTGGCTG TTATGGAGAC
    CTTTACTGCA AAGCATTAAA AACTTACAAC ATGCTGTGCT TTGGTATATA CCGACTCCGA
    GATGCCCACC TCAGCACACC CAGCCAGTGT ACCAAAAGGT ACGTTATCAC CAACCCTCCA
    TATGAGTTTG AACTGGTTCC CACAGATCTC ATCTTTTGCC TGATGCAGTT CGACCATAAT
    GCCAGCCAGT CCCGAGCTAG CCTGTCTCAC TCTTCACACT CCTCGCACTC ATCTAGCAAA
    AAAAGTTCAT CTGTTACCTC CATTCTACAC ACAGCCTCAG CCAACCGTCA GAACAGAGTC
    AAGGCTCGAG ATTCCCGTGA CAAACAAAAG ACGAATAGAA TGGGCCAAGC AGAAAAGAAA
    TGGTATACAG ATGAAACGGA AAACACTTAT CCCAGAAACA TTCAGATTAA GCCAATGAGC
    ACACATATGG CAAATCAGAT TAATCAATAC AAATCAACAA GCAGCTTGAT ACCGCCAATA
    CGAGAGGTCG AAGATGAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761905.1
    CDS1048..4671
    Translation

    Target ORF information:

    RefSeq Version XM_031906045.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TTGAAGATGA GCAACCATCA
    GCCTTGTCAC CAAAAAAAAA GCAACGAAAT GGAGGAATGA GGCAATCTCC AAACTCTTCA
    CCTAACATGA TGAGGCATGA TCCCCTTCTT ATGACTGGAA ATGATCAAAT TGATAATATG
    GATTCTAGCA GTGTTAAAAG ATATGATTCT ACTGGTATGT TCCACTGGTG TCCAGCCAAG
    GAATTGGATA AAGTGCTTCT GACACGGAGT GAAGCTGCCA TGACAGTCCT CAGTGGGCAT
    GTGGTTGTCT GCATTTTTGG AGACATGACA TCGGCACTGA TTGGAGTTCG GAATTTGGTG
    ATGCCACTGA GAGCCAGCAA TTTTCATTAC CATGAGTTGA AACATATAGT CTTTGTTGGG
    TCCCTTGATT ACCTTAAAAG AGAATGGGAA ACACTACACA ACTTCCCAAA GGTTTCCATC
    TTGCCTGGCA CACCATTAAG TCGAGCAGAT CTGAGAGCTG TCAACATTAA CCTGTGTGAC
    ATGTGCGTTA TTCTGTCAGC CAATCAGAAT AATATTGATG ATACATCACT GCAGGACAAA
    GAATGCATTT TGGCATCTCT CAACATCAAA TCCATGCAGT TTGATGACAG CATAGGGCTC
    TTGCAAGCTA ACTCTCAAGG GTTTACGCCC CCAGGAATGG AGAGATCCTC ACCTGATAAT
    AGCCCAGTGC ATGGTGTTGC AAGACAGGCA TCCATAACTA CAGGGGCCAA CATTCCCATA
    ATTACAGAAC TTGTGAACGA CTCAAATGTT CAGTTCTTGG ACCAGGATGA TGATGATGAC
    CCAGATACAG AGTTATACTT GACTCAGCCC TTCGCCTGTG GGACCGCCTT TGCTGTCAGT
    GTGTTAGACT CCCTTATGAG TGCAACATAC TTCAATGATA ACATTCTGAC TCTAATCAGA
    ACTCTGGTGA CTGGGGGAGC AACACCAGAG CTGGAAGCCC TCATTGCAGA AGAGAACGCT
    CTGCGTGGGG GTTATAGCAC CCCACAAACT CTAGCAAACA GAGACCGCTG TCGAGTAGCC
    CAATTAGCCT TGTATGATGG ACCATTTGCA GATTTGGGGG ATGGTGGCTG TTATGGAGAC
    CTTTACTGCA AAGCATTAAA AACTTACAAC ATGCTGTGCT TTGGTATATA CCGACTCCGA
    GATGCCCACC TCAGCACACC CAGCCAGTGT ACCAAAAGGT ACGTTATCAC CAACCCTCCA
    TATGAGTTTG AACTGGTTCC CACAGATCTC ATCTTTTGCC TGATGCAGTT CGACCATAAT
    GCCAGCCAGT CCCGAGCTAG CCTGTCTCAC TCTTCACACT CCTCGCACTC ATCTAGCAAA
    AAAAGTTCAT CTGTTACCTC CATTCTACAC ACAGCCTCAG CCAACCGTCA GAACAGAGTC
    AAGGCTCGAG ATTCCCGTGA CAAACAAAAG ACGAATAGAA TGGGCCAAGC AGAAAAGAAA
    TGGTATACAG ATGAAACGGA AAACACTTAT CCCAGAAACA TTCAGATTAA GCCAATGAGC
    ACACATATGG CAAATCAGAT TAATCAATAC AAATCAACAA GCAGCTTGAT ACCGCCAATA
    CGAGAGGTCG AAGATGAATG TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62488
    Clone ID Related Accession (Same CDS sequence) XM_031906046.1
    Accession Version XM_031906046.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT ATTTATTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG TGAACGACTC AAATGTTCAG TTCTTGGACC AGGATGATGA TGATGACCCA
    GATACAGAGT TATACTTGAC TCAGCCCTTC GCCTGTGGGA CCGCCTTTGC TGTCAGTGTG
    TTAGACTCCC TTATGAGTGC AACATACTTC AATGATAACA TTCTGACTCT AATCAGAACT
    CTGGTGACTG GGGGAGCAAC ACCAGAGCTG GAAGCCCTCA TTGCAGAAGA GAACGCTCTG
    CGTGGGGGTT ATAGCACCCC ACAAACTCTA GCAAACAGAG ACCGCTGTCG AGTAGCCCAA
    TTAGCCTTGT ATGATGGACC ATTTGCAGAT TTGGGGGATG GTGGCTGTTA TGGAGACCTT
    TACTGCAAAG CATTAAAAAC TTACAACATG CTGTGCTTTG GTATATACCG ACTCCGAGAT
    GCCCACCTCA GCACACCCAG CCAGTGTACC AAAAGGTACG TTATCACCAA CCCTCCATAT
    GAGTTTGAAC TGGTTCCCAC AGATCTCATC TTTTGCCTGA TGCAGTTCGA CCATAATGCC
    AGCCAGTCCC GAGCTAGCCT GTCTCACTCT TCACACTCCT CGCACTCATC TAGCAAAAAA
    AGTTCATCTG TTACCTCCAT TCTACACACA GCCTCAGCCA ACCGTCAGAA CAGAGTCAAG
    GCTCGAGATT CCCGTGACAA ACAAAAGACG AATAGAATGG GCCAAGCAGA AAAGAAATGG
    TATACAGATG AAACGGAAAA CACTTATCCC AGAAACATTC AGATTAAGCC AATGAGCACA
    CATATGGCAA ATCAGATTAA TCAATACAAA TCAACAAGCA GCTTGATACC GCCAATACGA
    GAGGTCGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761906.1
    CDS1047..4667
    Translation

    Target ORF information:

    RefSeq Version XM_031906046.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906046.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT ATTTATTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG TGAACGACTC AAATGTTCAG TTCTTGGACC AGGATGATGA TGATGACCCA
    GATACAGAGT TATACTTGAC TCAGCCCTTC GCCTGTGGGA CCGCCTTTGC TGTCAGTGTG
    TTAGACTCCC TTATGAGTGC AACATACTTC AATGATAACA TTCTGACTCT AATCAGAACT
    CTGGTGACTG GGGGAGCAAC ACCAGAGCTG GAAGCCCTCA TTGCAGAAGA GAACGCTCTG
    CGTGGGGGTT ATAGCACCCC ACAAACTCTA GCAAACAGAG ACCGCTGTCG AGTAGCCCAA
    TTAGCCTTGT ATGATGGACC ATTTGCAGAT TTGGGGGATG GTGGCTGTTA TGGAGACCTT
    TACTGCAAAG CATTAAAAAC TTACAACATG CTGTGCTTTG GTATATACCG ACTCCGAGAT
    GCCCACCTCA GCACACCCAG CCAGTGTACC AAAAGGTACG TTATCACCAA CCCTCCATAT
    GAGTTTGAAC TGGTTCCCAC AGATCTCATC TTTTGCCTGA TGCAGTTCGA CCATAATGCC
    AGCCAGTCCC GAGCTAGCCT GTCTCACTCT TCACACTCCT CGCACTCATC TAGCAAAAAA
    AGTTCATCTG TTACCTCCAT TCTACACACA GCCTCAGCCA ACCGTCAGAA CAGAGTCAAG
    GCTCGAGATT CCCGTGACAA ACAAAAGACG AATAGAATGG GCCAAGCAGA AAAGAAATGG
    TATACAGATG AAACGGAAAA CACTTATCCC AGAAACATTC AGATTAAGCC AATGAGCACA
    CATATGGCAA ATCAGATTAA TCAATACAAA TCAACAAGCA GCTTGATACC GCCAATACGA
    GAGGTCGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62489
    Clone ID Related Accession (Same CDS sequence) XM_031906048.1
    Accession Version XM_031906048.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GTGAACGACT CAAATGTTCA GTTCTTGGAC CAGGATGATG ATGATGACCC AGATACAGAG
    TTATACTTGA CTCAGCCCTT CGCCTGTGGG ACCGCCTTTG CTGTCAGTGT GTTAGACTCC
    CTTATGAGTG CAACATACTT CAATGATAAC ATTCTGACTC TAATCAGAAC TCTGGTGACT
    GGGGGAGCAA CACCAGAGCT GGAAGCCCTC ATTGCAGAAG AGAACGCTCT GCGTGGGGGT
    TATAGCACCC CACAAACTCT AGCAAACAGA GACCGCTGTC GAGTAGCCCA ATTAGCCTTG
    TATGATGGAC CATTTGCAGA TTTGGGGGAT GGTGGCTGTT ATGGAGACCT TTACTGCAAA
    GCATTAAAAA CTTACAACAT GCTGTGCTTT GGTATATACC GACTCCGAGA TGCCCACCTC
    AGCACACCCA GCCAGTGTAC CAAAAGGTAC GTTATCACCA ACCCTCCATA TGAGTTTGAA
    CTGGTTCCCA CAGATCTCAT CTTTTGCCTG ATGCAGTTCG ACCATAATGC CAGCCAGTCC
    CGAGCTAGCC TGTCTCACTC TTCACACTCC TCGCACTCAT CTAGCAAAAA AAGTTCATCT
    GTTACCTCCA TTCTACACAC AGCCTCAGCC AACCGTCAGA ACAGAGTCAA GGCTCGAGAT
    TCCCGTGACA AACAAAAGTA TGTTCTGGAT GACGCAATGC TGGGGAGAGG GGTGAGGAGG
    ACAGGGGGAG CGATGTCTAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761908.1
    CDS1046..4549
    Translation

    Target ORF information:

    RefSeq Version XM_031906048.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906048.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GTGAACGACT CAAATGTTCA GTTCTTGGAC CAGGATGATG ATGATGACCC AGATACAGAG
    TTATACTTGA CTCAGCCCTT CGCCTGTGGG ACCGCCTTTG CTGTCAGTGT GTTAGACTCC
    CTTATGAGTG CAACATACTT CAATGATAAC ATTCTGACTC TAATCAGAAC TCTGGTGACT
    GGGGGAGCAA CACCAGAGCT GGAAGCCCTC ATTGCAGAAG AGAACGCTCT GCGTGGGGGT
    TATAGCACCC CACAAACTCT AGCAAACAGA GACCGCTGTC GAGTAGCCCA ATTAGCCTTG
    TATGATGGAC CATTTGCAGA TTTGGGGGAT GGTGGCTGTT ATGGAGACCT TTACTGCAAA
    GCATTAAAAA CTTACAACAT GCTGTGCTTT GGTATATACC GACTCCGAGA TGCCCACCTC
    AGCACACCCA GCCAGTGTAC CAAAAGGTAC GTTATCACCA ACCCTCCATA TGAGTTTGAA
    CTGGTTCCCA CAGATCTCAT CTTTTGCCTG ATGCAGTTCG ACCATAATGC CAGCCAGTCC
    CGAGCTAGCC TGTCTCACTC TTCACACTCC TCGCACTCAT CTAGCAAAAA AAGTTCATCT
    GTTACCTCCA TTCTACACAC AGCCTCAGCC AACCGTCAGA ACAGAGTCAA GGCTCGAGAT
    TCCCGTGACA AACAAAAGTA TGTTCTGGAT GACGCAATGC TGGGGAGAGG GGTGAGGAGG
    ACAGGGGGAG CGATGTCTAT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa62490
    Clone ID Related Accession (Same CDS sequence) XM_031906049.1
    Accession Version XM_031906049.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TTATTTATTC CAAGATGTCC
    ACCCGCAAGA GGCTGATACA GGCATGTTGT ATGGGGTGCT CTGAGATAGA CTGCTCTTGC
    ATGTCAGGCA CTTTGCGTAG TAACATGGGA ACCCTTGAAC AAGCCTTCCC AATTTCTCCT
    GTCTCTGTTA ATGATTTCTC CACCAGTTTA CGTGGCTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG TGAACGACTC AAATGTTCAG TTCTTGGACC AGGATGATGA TGATGACCCA
    GATACAGAGT TATACTTGAC TCAGCCCTTC GCCTGTGGGA CCGCCTTTGC TGTCAGTGTG
    TTAGACTCCC TTATGAGTGC AACATACTTC AATGATAACA TTCTGACTCT AATCAGAACT
    CTGGTGACTG GGGGAGCAAC ACCAGAGCTG GAAGCCCTCA TTGCAGAAGA GAACGCTCTG
    CGTGGGGGTT ATAGCACCCC ACAAACTCTA GCAAACAGAG ACCGCTGTCG AGTAGCCCAA
    TTAGCCTTGT ATGATGGACC ATTTGCAGAT TTGGGGGATG GTGGCTGTTA TGGAGACCTT
    TACTGCAAAG CATTAAAAAC TTACAACATG CTGTGCTTTG GTATATACCG ACTCCGAGAT
    GCCCACCTCA GCACACCCAG CCAGTGTACC AAAAGGTACG TTATCACCAA CCCTCCATAT
    GAGTTTGAAC TGGTTCCCAC AGATCTCATC TTTTGCCTGA TGCAGTTCGA CCATAATGCC
    AGCCAGTCCC GAGCTAGCCT GTCTCACTCT TCACACTCCT CGCACTCATC TAGCAAAAAA
    AGTTCATCTG TTACCTCCAT TCTACACACA GCCTCAGCCA ACCGTCAGAA CAGAGTCAAG
    GCTCGAGATT CCCGTGACAA ACAAAAGACG AATAGAATGG GCCAAGAAAA GAAATGGTAT
    ACAGATGAAA CGGAAAACAC TTATCCCAGA AACATTCAGA TTAAGCCAAT GAGCACACAT
    ATGGCAAATC AGATTAATCA ATACAAATCA ACAAGCAGCT TGATACCGCC AATACGAGAG
    GTCGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761909.1
    CDS1054..4851
    Translation

    Target ORF information:

    RefSeq Version XM_031906049.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium calcium-activated channel subfamily M alpha 1 (kcnma1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031906049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGAAGCAG AAAGCGGATC
    ATAATAAATC CGGGTAACCA TGTGAAAATA AAAGAAGGTA CCCTGGGATT TTTTATTGCC
    AGTGATGCCA AAGAAGTAAA ACGGGCCTTT TTTTACTGTA AAGCATGCCA CGATGACATC
    ACAGACCCAA AGAGGATAAA GAAATGCGCC TGCAAGCGAC TTATTTATTC CAAGATGTCC
    ACCCGCAAGA GGCTGATACA GGCATGTTGT ATGGGGTGCT CTGAGATAGA CTGCTCTTGC
    ATGTCAGGCA CTTTGCGTAG TAACATGGGA ACCCTTGAAC AAGCCTTCCC AATTTCTCCT
    GTCTCTGTTA ATGATTTCTC CACCAGTTTA CGTGGCTTTG AAGATGAGCA ACCATCAGCC
    TTGTCACCAA AAAAAAAGCA ACGAAATGGA GGAATGAGGC AATCTCCAAA CTCTTCACCT
    AACATGATGA GGCATGATCC CCTTCTTATG ACTGGAAATG ATCAAATTGA TAATATGGAT
    TCTAGCAGTG TTAAAAGATA TGATTCTACT GGTATGTTCC ACTGGTGTCC AGCCAAGGAA
    TTGGATAAAG TGCTTCTGAC ACGGAGTGAA GCTGCCATGA CAGTCCTCAG TGGGCATGTG
    GTTGTCTGCA TTTTTGGAGA CATGACATCG GCACTGATTG GAGTTCGGAA TTTGGTGATG
    CCACTGAGAG CCAGCAATTT TCATTACCAT GAGTTGAAAC ATATAGTCTT TGTTGGGTCC
    CTTGATTACC TTAAAAGAGA ATGGGAAACA CTACACAACT TCCCAAAGGT TTCCATCTTG
    CCTGGCACAC CATTAAGTCG AGCAGATCTG AGAGCTGTCA ACATTAACCT GTGTGACATG
    TGCGTTATTC TGTCAGCCAA TCAGAATAAT ATTGATGATA CATCACTGCA GGACAAAGAA
    TGCATTTTGG CATCTCTCAA CATCAAATCC ATGCAGTTTG ATGACAGCAT AGGGCTCTTG
    CAAGCTAACT CTCAAGGGTT TACGCCCCCA GGAATGGAGA GATCCTCACC TGATAATAGC
    CCAGTGCATG GTGTTGCAAG ACAGGCATCC ATAACTACAG GGGCCAACAT TCCCATAATT
    ACAGAACTTG TGAACGACTC AAATGTTCAG TTCTTGGACC AGGATGATGA TGATGACCCA
    GATACAGAGT TATACTTGAC TCAGCCCTTC GCCTGTGGGA CCGCCTTTGC TGTCAGTGTG
    TTAGACTCCC TTATGAGTGC AACATACTTC AATGATAACA TTCTGACTCT AATCAGAACT
    CTGGTGACTG GGGGAGCAAC ACCAGAGCTG GAAGCCCTCA TTGCAGAAGA GAACGCTCTG
    CGTGGGGGTT ATAGCACCCC ACAAACTCTA GCAAACAGAG ACCGCTGTCG AGTAGCCCAA
    TTAGCCTTGT ATGATGGACC ATTTGCAGAT TTGGGGGATG GTGGCTGTTA TGGAGACCTT
    TACTGCAAAG CATTAAAAAC TTACAACATG CTGTGCTTTG GTATATACCG ACTCCGAGAT
    GCCCACCTCA GCACACCCAG CCAGTGTACC AAAAGGTACG TTATCACCAA CCCTCCATAT
    GAGTTTGAAC TGGTTCCCAC AGATCTCATC TTTTGCCTGA TGCAGTTCGA CCATAATGCC
    AGCCAGTCCC GAGCTAGCCT GTCTCACTCT TCACACTCCT CGCACTCATC TAGCAAAAAA
    AGTTCATCTG TTACCTCCAT TCTACACACA GCCTCAGCCA ACCGTCAGAA CAGAGTCAAG
    GCTCGAGATT CCCGTGACAA ACAAAAGACG AATAGAATGG GCCAAGAAAA GAAATGGTAT
    ACAGATGAAA CGGAAAACAC TTATCCCAGA AACATTCAGA TTAAGCCAAT GAGCACACAT
    ATGGCAAATC AGATTAATCA ATACAAATCA ACAAGCAGCT TGATACCGCC AATACGAGAG
    GTCGAAGATG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa12981
    Clone ID Related Accession (Same CDS sequence) XM_004916014.4 , XM_004916014.3
    Accession Version XM_004916014.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916014.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCT ACAAGGGAAG AAAATAGATT AAGTATCCAC
    AGGCTTTCTA TTCATCCCCA GACAGCCAAA GCTAGCTATT CTGTGGCCAG TTCCAAACTC
    TGCACAGAAC AGCAGGAGCC TGTGCCTCTT GTTAATAACC GCAAAGGAAG CCTGTTCTTG
    CCATGTGATT CCTCTCTCCT GCACCTGCAG TTACTGGGTT CCTCTGGCAC TGAACACCGT
    ACCAGTATTA AATTGCAGCG TGCACTGAGC TTGCCAGGCA AATACCGCTA CCACCCCAAT
    CAGCCAGTAC TCATCCAAAA GCAGTTTGAA GATGAGCAAC CATCAGCCTT GTCACCAAAA
    AAAAAGCAAC GAAATGGAGG AATGAGGCAA TCTCCAAACT CTTCACCTAA CATGATGAGG
    CATGATCCCC TTCTTATGAC TGGAAATGAT CAAATTGATA ATATGGATTC TAGCAGTGTT
    AAAAGATATG ATTCTACTGG TATGTTCCAC TGGTGTCCAG CCAAGGAATT GGATAAAGTG
    CTTCTGACAC GGAGTGAAGC TGCCATGACA GTCCTCAGTG GGCATGTGGT TGTCTGCATT
    TTTGGAGACA TGACATCGGC ACTGATTGGA GTTCGGAATT TGGTGATGCC ACTGAGAGCC
    AGCAATTTTC ATTACCATGA GTTGAAACAT ATAGTCTTTG TTGGGTCCCT TGATTACCTT
    AAAAGAGAAT GGGAAACACT ACACAACTTC CCAAAGGTTT CCATCTTGCC TGGCACACCA
    TTAAGTCGAG CAGATCTGAG AGCTGTCAAC ATTAACCTGT GTGACATGTG CGTTATTCTG
    TCAGCCAATC AGAATAATAT TGATGATACA TCACTGCAGG ACAAAGAATG CATTTTGGCA
    TCTCTCAACA TCAAATCCAT GCAGTTTGAT GACAGCATAG GGCTCTTGCA AGCTAACTCT
    CAAGGGTTTA CGCCCCCAGG AATGGAGAGA TCCTCACCTG ATAATAGCCC AGTGCATGGT
    GTTGCAAGAC AGGCATCCAT AACTACAGGG GCCAACATTC CCATAATTAC AGAACTTGCT
    AAGCCTGGCA AACTGCTGCC TTTGATTTCA TTCAGTCAGG AAAAAAACAG TGGGACACAC
    ATACCAATTA TTACAGAGTT GGTGAACGAC TCAAATGTTC AGTTCTTGGA CCAGGATGAT
    GATGATGACC CAGATACAGA GTTATACTTG ACTCAGCCCT TCGCCTGTGG GACCGCCTTT
    GCTGTCAGTG TGTTAGACTC CCTTATGAGT GCAACATACT TCAATGATAA CATTCTGACT
    CTAATCAGAA CTCTGGTGAC TGGGGGAGCA ACACCAGAGC TGGAAGCCCT CATTGCAGAA
    GAGAACGCTC TGCGTGGGGG TTATAGCACC CCACAAACTC TAGCAAACAG AGACCGCTGT
    CGAGTAGCCC AATTAGCCTT GTATGATGGA CCATTTGCAG ATTTGGGGGA TGGTGGCTGT
    TATGGAGACC TTTACTGCAA AGCATTAAAA ACTTACAACA TGCTGTGCTT TGGTATATAC
    CGACTCCGAG ATGCCCACCT CAGCACACCC AGCCAGTGTA CCAAAAGGTA CGTTATCACC
    AACCCTCCAT ATGAGTTTGA ACTGGTTCCC ACAGATCTCA TCTTTTGCCT GATGCAGTTC
    GACCATAATG CCAGCCAGTC CCGAGCTAGC CTGTCTCACT CTTCACACTC CTCGCACTCA
    TCTAGCAAAA AAAGTTCATC TGTTACCTCC ATTCTACACA CAGCCTCAGC CAACCGTCAG
    AACAGAGTCA AGGCTCGAGA TTCCCGTGAC AAACAAAAGA CGAATAGAAT GGGCCAAGCA
    GAAAAGAAAT GGTATACAGA TGAAACGGAA AACACTTATC CCAGAAACAT TCAGATTAAG
    CCAATGAGCA CACATATGGC AAATCAGATT AATCAATACA AATCAACAAG CAGCTTGATA
    CCGCCAATAC GAGAGGTCGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916071.1
    CDS1503..5495
    Translation

    Target ORF information:

    RefSeq Version XM_004916014.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916014.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCT ACAAGGGAAG AAAATAGATT AAGTATCCAC
    AGGCTTTCTA TTCATCCCCA GACAGCCAAA GCTAGCTATT CTGTGGCCAG TTCCAAACTC
    TGCACAGAAC AGCAGGAGCC TGTGCCTCTT GTTAATAACC GCAAAGGAAG CCTGTTCTTG
    CCATGTGATT CCTCTCTCCT GCACCTGCAG TTACTGGGTT CCTCTGGCAC TGAACACCGT
    ACCAGTATTA AATTGCAGCG TGCACTGAGC TTGCCAGGCA AATACCGCTA CCACCCCAAT
    CAGCCAGTAC TCATCCAAAA GCAGTTTGAA GATGAGCAAC CATCAGCCTT GTCACCAAAA
    AAAAAGCAAC GAAATGGAGG AATGAGGCAA TCTCCAAACT CTTCACCTAA CATGATGAGG
    CATGATCCCC TTCTTATGAC TGGAAATGAT CAAATTGATA ATATGGATTC TAGCAGTGTT
    AAAAGATATG ATTCTACTGG TATGTTCCAC TGGTGTCCAG CCAAGGAATT GGATAAAGTG
    CTTCTGACAC GGAGTGAAGC TGCCATGACA GTCCTCAGTG GGCATGTGGT TGTCTGCATT
    TTTGGAGACA TGACATCGGC ACTGATTGGA GTTCGGAATT TGGTGATGCC ACTGAGAGCC
    AGCAATTTTC ATTACCATGA GTTGAAACAT ATAGTCTTTG TTGGGTCCCT TGATTACCTT
    AAAAGAGAAT GGGAAACACT ACACAACTTC CCAAAGGTTT CCATCTTGCC TGGCACACCA
    TTAAGTCGAG CAGATCTGAG AGCTGTCAAC ATTAACCTGT GTGACATGTG CGTTATTCTG
    TCAGCCAATC AGAATAATAT TGATGATACA TCACTGCAGG ACAAAGAATG CATTTTGGCA
    TCTCTCAACA TCAAATCCAT GCAGTTTGAT GACAGCATAG GGCTCTTGCA AGCTAACTCT
    CAAGGGTTTA CGCCCCCAGG AATGGAGAGA TCCTCACCTG ATAATAGCCC AGTGCATGGT
    GTTGCAAGAC AGGCATCCAT AACTACAGGG GCCAACATTC CCATAATTAC AGAACTTGCT
    AAGCCTGGCA AACTGCTGCC TTTGATTTCA TTCAGTCAGG AAAAAAACAG TGGGACACAC
    ATACCAATTA TTACAGAGTT GGTGAACGAC TCAAATGTTC AGTTCTTGGA CCAGGATGAT
    GATGATGACC CAGATACAGA GTTATACTTG ACTCAGCCCT TCGCCTGTGG GACCGCCTTT
    GCTGTCAGTG TGTTAGACTC CCTTATGAGT GCAACATACT TCAATGATAA CATTCTGACT
    CTAATCAGAA CTCTGGTGAC TGGGGGAGCA ACACCAGAGC TGGAAGCCCT CATTGCAGAA
    GAGAACGCTC TGCGTGGGGG TTATAGCACC CCACAAACTC TAGCAAACAG AGACCGCTGT
    CGAGTAGCCC AATTAGCCTT GTATGATGGA CCATTTGCAG ATTTGGGGGA TGGTGGCTGT
    TATGGAGACC TTTACTGCAA AGCATTAAAA ACTTACAACA TGCTGTGCTT TGGTATATAC
    CGACTCCGAG ATGCCCACCT CAGCACACCC AGCCAGTGTA CCAAAAGGTA CGTTATCACC
    AACCCTCCAT ATGAGTTTGA ACTGGTTCCC ACAGATCTCA TCTTTTGCCT GATGCAGTTC
    GACCATAATG CCAGCCAGTC CCGAGCTAGC CTGTCTCACT CTTCACACTC CTCGCACTCA
    TCTAGCAAAA AAAGTTCATC TGTTACCTCC ATTCTACACA CAGCCTCAGC CAACCGTCAG
    AACAGAGTCA AGGCTCGAGA TTCCCGTGAC AAACAAAAGA CGAATAGAAT GGGCCAAGCA
    GAAAAGAAAT GGTATACAGA TGAAACGGAA AACACTTATC CCAGAAACAT TCAGATTAAG
    CCAATGAGCA CACATATGGC AAATCAGATT AATCAATACA AATCAACAAG CAGCTTGATA
    CCGCCAATAC GAGAGGTCGA AGATGAATGT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35188
    Clone ID Related Accession (Same CDS sequence) XM_012966933.2
    Accession Version XM_012966933.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCT ACAAGGGAAG AAAATAGATT AAGTATCCAC
    AGGCTTTCTA TTCATCCCCA GACAGCCAAA GCTAGCTATT CTGTGGCCAG TTCCAAACTC
    TGCACAGAAC AGCAGGAGCC TGTGCCTCTT GTTAATAACC GCAAAGGAAG CCTGTTCTTG
    CCATGTGATT CCTCTCTCCT GCACCTGCAG TTACTGGGTT CCTCTGGCAC TGAACACCGT
    ACCAGTATTA AATTGCAGCG TGCACTGAGC TTGCCAGGCA AATACCGCTA CCACCCCAAT
    CAGCCAGTAC TCATCCAAAA GCAGTTTGAA GATGAGCAAC CATCAGCCTT GTCACCAAAA
    AAAAAGCAAC GAAATGGAGG AATGAGGCAA TCTCCAAACT CTTCACCTAA CATGATGAGG
    CATGATCCCC TTCTTATGAC TGGAAATGAT CAAATTGATA ATATGGATTC TAGCAGTGTT
    AAAAGATATG ATTCTACTGG TATGTTCCAC TGGTGTCCAG CCAAGGAATT GGATAAAGTG
    CTTCTGACAC GGAGTGAAGC TGCCATGACA GTCCTCAGTG GGCATGTGGT TGTCTGCATT
    TTTGGAGACA TGACATCGGC ACTGATTGGA GTTCGGAATT TGGTGATGCC ACTGAGAGCC
    AGCAATTTTC ATTACCATGA GTTGAAACAT ATAGTCTTTG TTGGGTCCCT TGATTACCTT
    AAAAGAGAAT GGGAAACACT ACACAACTTC CCAAAGGTTT CCATCTTGCC TGGCACACCA
    TTAAGTCGAG CAGATCTGAG AGCTGTCAAC ATTAACCTGT GTGACATGTG CGTTATTCTG
    TCAGCCAATC AGAATAATAT TGATGATACA TCACTGCAGG ACAAAGAATG CATTTTGGCA
    TCTCTCAACA TCAAATCCAT GCAGTTTGAT GACAGCATAG GGCTCTTGCA AGCTAACTCT
    CAAGGGTTTA CGCCCCCAGG AATGGAGAGA TCCTCACCTG ATAATAGCCC AGTGCATGGT
    GTTGCAAGAC AGGCATCCAT AACTACAGGG GCCAACATTC CCATAATTAC AGAACTTGTG
    AACGACTCAA ATGTTCAGTT CTTGGACCAG GATGATGATG ATGACCCAGA TACAGAGTTA
    TACTTGACTC AGCCCTTCGC CTGTGGGACC GCCTTTGCTG TCAGTGTGTT AGACTCCCTT
    ATGAGTGCAA CATACTTCAA TGATAACATT CTGACTCTAA TCAGAACTCT GGTGACTGGG
    GGAGCAACAC CAGAGCTGGA AGCCCTCATT GCAGAAGAGA ACGCTCTGCG TGGGGGTTAT
    AGCACCCCAC AAACTCTAGC AAACAGAGAC CGCTGTCGAG TAGCCCAATT AGCCTTGTAT
    GATGGACCAT TTGCAGATTT GGGGGATGGT GGCTGTTATG GAGACCTTTA CTGCAAAGCA
    TTAAAAACTT ACAACATGCT GTGCTTTGGT ATATACCGAC TCCGAGATGC CCACCTCAGC
    ACACCCAGCC AGTGTACCAA AAGGTACGTT ATCACCAACC CTCCATATGA GTTTGAACTG
    GTTCCCACAG ATCTCATCTT TTGCCTGATG CAGTTCGACC ATAATGCCAG CCAGTCCCGA
    GCTAGCCTGT CTCACTCTTC ACACTCCTCG CACTCATCTA GCAAAAAAAG TTCATCTGTT
    ACCTCCATTC TACACACAGC CTCAGCCAAC CGTCAGAACA GAGTCAAGGC TCGAGATTCC
    CGTGACAAAC AAAAGACGAA TAGAATGGGC CAAGCAGAAA AGAAATGGTA TACAGATGAA
    ACGGAAAACA CTTATCCCAG AAACATTCAG ATTAAGCCAA TGAGCACACA TATGGCAAAT
    CAGATTAATC AATACAAATC AACAAGCAGC TTGATACCGC CAATACGAGA GGTCGAAGAT
    GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822387.1
    CDS1500..5408
    Translation

    Target ORF information:

    RefSeq Version XM_012966933.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966933.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCT ACAAGGGAAG AAAATAGATT AAGTATCCAC
    AGGCTTTCTA TTCATCCCCA GACAGCCAAA GCTAGCTATT CTGTGGCCAG TTCCAAACTC
    TGCACAGAAC AGCAGGAGCC TGTGCCTCTT GTTAATAACC GCAAAGGAAG CCTGTTCTTG
    CCATGTGATT CCTCTCTCCT GCACCTGCAG TTACTGGGTT CCTCTGGCAC TGAACACCGT
    ACCAGTATTA AATTGCAGCG TGCACTGAGC TTGCCAGGCA AATACCGCTA CCACCCCAAT
    CAGCCAGTAC TCATCCAAAA GCAGTTTGAA GATGAGCAAC CATCAGCCTT GTCACCAAAA
    AAAAAGCAAC GAAATGGAGG AATGAGGCAA TCTCCAAACT CTTCACCTAA CATGATGAGG
    CATGATCCCC TTCTTATGAC TGGAAATGAT CAAATTGATA ATATGGATTC TAGCAGTGTT
    AAAAGATATG ATTCTACTGG TATGTTCCAC TGGTGTCCAG CCAAGGAATT GGATAAAGTG
    CTTCTGACAC GGAGTGAAGC TGCCATGACA GTCCTCAGTG GGCATGTGGT TGTCTGCATT
    TTTGGAGACA TGACATCGGC ACTGATTGGA GTTCGGAATT TGGTGATGCC ACTGAGAGCC
    AGCAATTTTC ATTACCATGA GTTGAAACAT ATAGTCTTTG TTGGGTCCCT TGATTACCTT
    AAAAGAGAAT GGGAAACACT ACACAACTTC CCAAAGGTTT CCATCTTGCC TGGCACACCA
    TTAAGTCGAG CAGATCTGAG AGCTGTCAAC ATTAACCTGT GTGACATGTG CGTTATTCTG
    TCAGCCAATC AGAATAATAT TGATGATACA TCACTGCAGG ACAAAGAATG CATTTTGGCA
    TCTCTCAACA TCAAATCCAT GCAGTTTGAT GACAGCATAG GGCTCTTGCA AGCTAACTCT
    CAAGGGTTTA CGCCCCCAGG AATGGAGAGA TCCTCACCTG ATAATAGCCC AGTGCATGGT
    GTTGCAAGAC AGGCATCCAT AACTACAGGG GCCAACATTC CCATAATTAC AGAACTTGTG
    AACGACTCAA ATGTTCAGTT CTTGGACCAG GATGATGATG ATGACCCAGA TACAGAGTTA
    TACTTGACTC AGCCCTTCGC CTGTGGGACC GCCTTTGCTG TCAGTGTGTT AGACTCCCTT
    ATGAGTGCAA CATACTTCAA TGATAACATT CTGACTCTAA TCAGAACTCT GGTGACTGGG
    GGAGCAACAC CAGAGCTGGA AGCCCTCATT GCAGAAGAGA ACGCTCTGCG TGGGGGTTAT
    AGCACCCCAC AAACTCTAGC AAACAGAGAC CGCTGTCGAG TAGCCCAATT AGCCTTGTAT
    GATGGACCAT TTGCAGATTT GGGGGATGGT GGCTGTTATG GAGACCTTTA CTGCAAAGCA
    TTAAAAACTT ACAACATGCT GTGCTTTGGT ATATACCGAC TCCGAGATGC CCACCTCAGC
    ACACCCAGCC AGTGTACCAA AAGGTACGTT ATCACCAACC CTCCATATGA GTTTGAACTG
    GTTCCCACAG ATCTCATCTT TTGCCTGATG CAGTTCGACC ATAATGCCAG CCAGTCCCGA
    GCTAGCCTGT CTCACTCTTC ACACTCCTCG CACTCATCTA GCAAAAAAAG TTCATCTGTT
    ACCTCCATTC TACACACAGC CTCAGCCAAC CGTCAGAACA GAGTCAAGGC TCGAGATTCC
    CGTGACAAAC AAAAGACGAA TAGAATGGGC CAAGCAGAAA AGAAATGGTA TACAGATGAA
    ACGGAAAACA CTTATCCCAG AAACATTCAG ATTAAGCCAA TGAGCACACA TATGGCAAAT
    CAGATTAATC AATACAAATC AACAAGCAGC TTGATACCGC CAATACGAGA GGTCGAAGAT
    GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35192
    Clone ID Related Accession (Same CDS sequence) XM_012966937.2 , XM_012966937.3
    Accession Version XM_012966937.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCC AAGATGTCCA CCCGCAAGAG GCTGATACAG
    GCATGTTGTA TGGGGTGCTC TGAGATAGAC TGCTCTTGCA TGTCAGGCAC TTTGCGTAGT
    AACATGGGAA CCCTTGAACA AGCCTTCCCA ATTTCTCCTG TCTCTGTTAA TGATTTCTCC
    ACCAGTTTAC GTGGCTTTGA AGATGAGCAA CCATCAGCCT TGTCACCAAA AAAAAAGCAA
    CGAAATGGAG GAATGAGGCA ATCTCCAAAC TCTTCACCTA ACATGATGAG GCATGATCCC
    CTTCTTATGA CTGGAAATGA TCAAATTGAT AATATGGATT CTAGCAGTGT TAAAAGATAT
    GATTCTACTG GTATGTTCCA CTGGTGTCCA GCCAAGGAAT TGGATAAAGT GCTTCTGACA
    CGGAGTGAAG CTGCCATGAC AGTCCTCAGT GGGCATGTGG TTGTCTGCAT TTTTGGAGAC
    ATGACATCGG CACTGATTGG AGTTCGGAAT TTGGTGATGC CACTGAGAGC CAGCAATTTT
    CATTACCATG AGTTGAAACA TATAGTCTTT GTTGGGTCCC TTGATTACCT TAAAAGAGAA
    TGGGAAACAC TACACAACTT CCCAAAGGTT TCCATCTTGC CTGGCACACC ATTAAGTCGA
    GCAGATCTGA GAGCTGTCAA CATTAACCTG TGTGACATGT GCGTTATTCT GTCAGCCAAT
    CAGAATAATA TTGATGATAC ATCACTGCAG GACAAAGAAT GCATTTTGGC ATCTCTCAAC
    ATCAAATCCA TGCAGTTTGA TGACAGCATA GGGCTCTTGC AAGCTAACTC TCAAGGGTTT
    ACGCCCCCAG GAATGGAGAG ATCCTCACCT GATAATAGCC CAGTGCATGG TGTTGCAAGA
    CAGGCATCCA TAACTACAGG GGCCAACATT CCCATAATTA CAGAACTTGT GAACGACTCA
    AATGTTCAGT TCTTGGACCA GGATGATGAT GATGACCCAG ATACAGAGTT ATACTTGACT
    CAGCCCTTCG CCTGTGGGAC CGCCTTTGCT GTCAGTGTGT TAGACTCCCT TATGAGTGCA
    ACATACTTCA ATGATAACAT TCTGACTCTA ATCAGAACTC TGGTGACTGG GGGAGCAACA
    CCAGAGCTGG AAGCCCTCAT TGCAGAAGAG AACGCTCTGC GTGGGGGTTA TAGCACCCCA
    CAAACTCTAG CAAACAGAGA CCGCTGTCGA GTAGCCCAAT TAGCCTTGTA TGATGGACCA
    TTTGCAGATT TGGGGGATGG TGGCTGTTAT GGAGACCTTT ACTGCAAAGC ATTAAAAACT
    TACAACATGC TGTGCTTTGG TATATACCGA CTCCGAGATG CCCACCTCAG CACACCCAGC
    CAGTGTACCA AAAGGTACGT TATCACCAAC CCTCCATATG AGTTTGAACT GGTTCCCACA
    GATCTCATCT TTTGCCTGAT GCAGTTCGAC CATAATGCCA GCCAGTCCCG AGCTAGCCTG
    TCTCACTCTT CACACTCCTC GCACTCATCT AGCAAAAAAA GTTCATCTGT TACCTCCATT
    CTACACACAG CCTCAGCCAA CCGTCAGAAC AGAGTCAAGG CTCGAGATTC CCGTGACAAA
    CAAAAGACGA ATAGAATGGG CCAAGCAGAA AAGAAATGGT ATACAGATGA AACGGAAAAC
    ACTTATCCCA GAAACATTCA GATTAAGCCA ATGAGCACAC ATATGGCAAA TCAGATTAAT
    CAATACAAAT CAACAAGCAG CTTGATACCG CCAATACGAG AGGTCGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822391.1
    CDS1498..5277
    Translation

    Target ORF information:

    RefSeq Version XM_012966937.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966937.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCC AAGATGTCCA CCCGCAAGAG GCTGATACAG
    GCATGTTGTA TGGGGTGCTC TGAGATAGAC TGCTCTTGCA TGTCAGGCAC TTTGCGTAGT
    AACATGGGAA CCCTTGAACA AGCCTTCCCA ATTTCTCCTG TCTCTGTTAA TGATTTCTCC
    ACCAGTTTAC GTGGCTTTGA AGATGAGCAA CCATCAGCCT TGTCACCAAA AAAAAAGCAA
    CGAAATGGAG GAATGAGGCA ATCTCCAAAC TCTTCACCTA ACATGATGAG GCATGATCCC
    CTTCTTATGA CTGGAAATGA TCAAATTGAT AATATGGATT CTAGCAGTGT TAAAAGATAT
    GATTCTACTG GTATGTTCCA CTGGTGTCCA GCCAAGGAAT TGGATAAAGT GCTTCTGACA
    CGGAGTGAAG CTGCCATGAC AGTCCTCAGT GGGCATGTGG TTGTCTGCAT TTTTGGAGAC
    ATGACATCGG CACTGATTGG AGTTCGGAAT TTGGTGATGC CACTGAGAGC CAGCAATTTT
    CATTACCATG AGTTGAAACA TATAGTCTTT GTTGGGTCCC TTGATTACCT TAAAAGAGAA
    TGGGAAACAC TACACAACTT CCCAAAGGTT TCCATCTTGC CTGGCACACC ATTAAGTCGA
    GCAGATCTGA GAGCTGTCAA CATTAACCTG TGTGACATGT GCGTTATTCT GTCAGCCAAT
    CAGAATAATA TTGATGATAC ATCACTGCAG GACAAAGAAT GCATTTTGGC ATCTCTCAAC
    ATCAAATCCA TGCAGTTTGA TGACAGCATA GGGCTCTTGC AAGCTAACTC TCAAGGGTTT
    ACGCCCCCAG GAATGGAGAG ATCCTCACCT GATAATAGCC CAGTGCATGG TGTTGCAAGA
    CAGGCATCCA TAACTACAGG GGCCAACATT CCCATAATTA CAGAACTTGT GAACGACTCA
    AATGTTCAGT TCTTGGACCA GGATGATGAT GATGACCCAG ATACAGAGTT ATACTTGACT
    CAGCCCTTCG CCTGTGGGAC CGCCTTTGCT GTCAGTGTGT TAGACTCCCT TATGAGTGCA
    ACATACTTCA ATGATAACAT TCTGACTCTA ATCAGAACTC TGGTGACTGG GGGAGCAACA
    CCAGAGCTGG AAGCCCTCAT TGCAGAAGAG AACGCTCTGC GTGGGGGTTA TAGCACCCCA
    CAAACTCTAG CAAACAGAGA CCGCTGTCGA GTAGCCCAAT TAGCCTTGTA TGATGGACCA
    TTTGCAGATT TGGGGGATGG TGGCTGTTAT GGAGACCTTT ACTGCAAAGC ATTAAAAACT
    TACAACATGC TGTGCTTTGG TATATACCGA CTCCGAGATG CCCACCTCAG CACACCCAGC
    CAGTGTACCA AAAGGTACGT TATCACCAAC CCTCCATATG AGTTTGAACT GGTTCCCACA
    GATCTCATCT TTTGCCTGAT GCAGTTCGAC CATAATGCCA GCCAGTCCCG AGCTAGCCTG
    TCTCACTCTT CACACTCCTC GCACTCATCT AGCAAAAAAA GTTCATCTGT TACCTCCATT
    CTACACACAG CCTCAGCCAA CCGTCAGAAC AGAGTCAAGG CTCGAGATTC CCGTGACAAA
    CAAAAGACGA ATAGAATGGG CCAAGCAGAA AAGAAATGGT ATACAGATGA AACGGAAAAC
    ACTTATCCCA GAAACATTCA GATTAAGCCA ATGAGCACAC ATATGGCAAA TCAGATTAAT
    CAATACAAAT CAACAAGCAG CTTGATACCG CCAATACGAG AGGTCGAAGA TGAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35193
    Clone ID Related Accession (Same CDS sequence) XM_012966938.2
    Accession Version XM_012966938.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTC CTCCTCACTC TTCCTTGCCT GACCGCTTTG
    CCTGTGTTTG CAGTTGAAGA TGAGCAACCA TCAGCCTTGT CACCAAAAAA AAAGCAACGA
    AATGGAGGAA TGAGGCAATC TCCAAACTCT TCACCTAACA TGATGAGGCA TGATCCCCTT
    CTTATGACTG GAAATGATCA AATTGATAAT ATGGATTCTA GCAGTGTTAA AAGATATGAT
    TCTACTGGTA TGTTCCACTG GTGTCCAGCC AAGGAATTGG ATAAAGTGCT TCTGACACGG
    AGTGAAGCTG CCATGACAGT CCTCAGTGGG CATGTGGTTG TCTGCATTTT TGGAGACATG
    ACATCGGCAC TGATTGGAGT TCGGAATTTG GTGATGCCAC TGAGAGCCAG CAATTTTCAT
    TACCATGAGT TGAAACATAT AGTCTTTGTT GGGTCCCTTG ATTACCTTAA AAGAGAATGG
    GAAACACTAC ACAACTTCCC AAAGGTTTCC ATCTTGCCTG GCACACCATT AAGTCGAGCA
    GATCTGAGAG CTGTCAACAT TAACCTGTGT GACATGTGCG TTATTCTGTC AGCCAATCAG
    AATAATATTG ATGATACATC ACTGCAGGAC AAAGAATGCA TTTTGGCATC TCTCAACATC
    AAATCCATGC AGTTTGATGA CAGCATAGGG CTCTTGCAAG CTAACTCTCA AGGGTTTACG
    CCCCCAGGAA TGGAGAGATC CTCACCTGAT AATAGCCCAG TGCATGGTGT TGCAAGACAG
    GCATCCATAA CTACAGGGGC CAACATTCCC ATAATTACAG AACTTGCTAA GCCTGGCAAA
    CTGCTGCCTT TGATTTCATT CAGTCAGGAA AAAAACAGTG GGACACACAT ACCAATTATT
    ACAGAGTTGG TGAACGACTC AAATGTTCAG TTCTTGGACC AGGATGATGA TGATGACCCA
    GATACAGAGT TATACTTGAC TCAGCCCTTC GCCTGTGGGA CCGCCTTTGC TGTCAGTGTG
    TTAGACTCCC TTATGAGTGC AACATACTTC AATGATAACA TTCTGACTCT AATCAGAACT
    CTGGTGACTG GGGGAGCAAC ACCAGAGCTG GAAGCCCTCA TTGCAGAAGA GAACGCTCTG
    CGTGGGGGTT ATAGCACCCC ACAAACTCTA GCAAACAGAG ACCGCTGTCG AGTAGCCCAA
    TTAGCCTTGT ATGATGGACC ATTTGCAGAT TTGGGGGATG GTGGCTGTTA TGGAGACCTT
    TACTGCAAAG CATTAAAAAC TTACAACATG CTGTGCTTTG GTATATACCG ACTCCGAGAT
    GCCCACCTCA GCACACCCAG CCAGTGTACC AAAAGGTACG TTATCACCAA CCCTCCATAT
    GAGTTTGAAC TGGTTCCCAC AGATCTCATC TTTTGCCTGA TGCAGTTCGA CCATAATGCC
    AGCCAGTCCC GAGCTAGCCT GTCTCACTCT TCACACTCCT CGCACTCATC TAGCAAAAAA
    AGTTCATCTG TTACCTCCAT TCTACACACA GCCTCAGCCA ACCGTCAGAA CAGAGTCAAG
    GCTCGAGATT CCCGTGACAA ACAAAAGACG AATAGAATGG GCCAAGCAGA AAAGAAATGG
    TATACAGATG AAACGGAAAA CACTTATCCC AGAAACATTC AGATTAAGCC AATGAGCACA
    CATATGGCAA ATCAGATTAA TCAATACAAA TCAACAAGCA GCTTGATACC GCCAATACGA
    GAGGTCGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822392.1
    CDS1500..5240
    Translation

    Target ORF information:

    RefSeq Version XM_012966938.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966938.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTC CTCCTCACTC TTCCTTGCCT GACCGCTTTG
    CCTGTGTTTG CAGTTGAAGA TGAGCAACCA TCAGCCTTGT CACCAAAAAA AAAGCAACGA
    AATGGAGGAA TGAGGCAATC TCCAAACTCT TCACCTAACA TGATGAGGCA TGATCCCCTT
    CTTATGACTG GAAATGATCA AATTGATAAT ATGGATTCTA GCAGTGTTAA AAGATATGAT
    TCTACTGGTA TGTTCCACTG GTGTCCAGCC AAGGAATTGG ATAAAGTGCT TCTGACACGG
    AGTGAAGCTG CCATGACAGT CCTCAGTGGG CATGTGGTTG TCTGCATTTT TGGAGACATG
    ACATCGGCAC TGATTGGAGT TCGGAATTTG GTGATGCCAC TGAGAGCCAG CAATTTTCAT
    TACCATGAGT TGAAACATAT AGTCTTTGTT GGGTCCCTTG ATTACCTTAA AAGAGAATGG
    GAAACACTAC ACAACTTCCC AAAGGTTTCC ATCTTGCCTG GCACACCATT AAGTCGAGCA
    GATCTGAGAG CTGTCAACAT TAACCTGTGT GACATGTGCG TTATTCTGTC AGCCAATCAG
    AATAATATTG ATGATACATC ACTGCAGGAC AAAGAATGCA TTTTGGCATC TCTCAACATC
    AAATCCATGC AGTTTGATGA CAGCATAGGG CTCTTGCAAG CTAACTCTCA AGGGTTTACG
    CCCCCAGGAA TGGAGAGATC CTCACCTGAT AATAGCCCAG TGCATGGTGT TGCAAGACAG
    GCATCCATAA CTACAGGGGC CAACATTCCC ATAATTACAG AACTTGCTAA GCCTGGCAAA
    CTGCTGCCTT TGATTTCATT CAGTCAGGAA AAAAACAGTG GGACACACAT ACCAATTATT
    ACAGAGTTGG TGAACGACTC AAATGTTCAG TTCTTGGACC AGGATGATGA TGATGACCCA
    GATACAGAGT TATACTTGAC TCAGCCCTTC GCCTGTGGGA CCGCCTTTGC TGTCAGTGTG
    TTAGACTCCC TTATGAGTGC AACATACTTC AATGATAACA TTCTGACTCT AATCAGAACT
    CTGGTGACTG GGGGAGCAAC ACCAGAGCTG GAAGCCCTCA TTGCAGAAGA GAACGCTCTG
    CGTGGGGGTT ATAGCACCCC ACAAACTCTA GCAAACAGAG ACCGCTGTCG AGTAGCCCAA
    TTAGCCTTGT ATGATGGACC ATTTGCAGAT TTGGGGGATG GTGGCTGTTA TGGAGACCTT
    TACTGCAAAG CATTAAAAAC TTACAACATG CTGTGCTTTG GTATATACCG ACTCCGAGAT
    GCCCACCTCA GCACACCCAG CCAGTGTACC AAAAGGTACG TTATCACCAA CCCTCCATAT
    GAGTTTGAAC TGGTTCCCAC AGATCTCATC TTTTGCCTGA TGCAGTTCGA CCATAATGCC
    AGCCAGTCCC GAGCTAGCCT GTCTCACTCT TCACACTCCT CGCACTCATC TAGCAAAAAA
    AGTTCATCTG TTACCTCCAT TCTACACACA GCCTCAGCCA ACCGTCAGAA CAGAGTCAAG
    GCTCGAGATT CCCGTGACAA ACAAAAGACG AATAGAATGG GCCAAGCAGA AAAGAAATGG
    TATACAGATG AAACGGAAAA CACTTATCCC AGAAACATTC AGATTAAGCC AATGAGCACA
    CATATGGCAA ATCAGATTAA TCAATACAAA TCAACAAGCA GCTTGATACC GCCAATACGA
    GAGGTCGAAG ATGAATGTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35194
    Clone ID Related Accession (Same CDS sequence) XM_012966939.2 , XM_012966939.3
    Accession Version XM_012966939.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GCTAAGCCTG GCAAACTGCT GCCTTTGATT TCATTCAGTC AGGAAAAAAA CAGTGGGACA
    CACATACCAA TTATTACAGA GTTGGTGAAC GACTCAAATG TTCAGTTCTT GGACCAGGAT
    GATGATGATG ACCCAGATAC AGAGTTATAC TTGACTCAGC CCTTCGCCTG TGGGACCGCC
    TTTGCTGTCA GTGTGTTAGA CTCCCTTATG AGTGCAACAT ACTTCAATGA TAACATTCTG
    ACTCTAATCA GAACTCTGGT GACTGGGGGA GCAACACCAG AGCTGGAAGC CCTCATTGCA
    GAAGAGAACG CTCTGCGTGG GGGTTATAGC ACCCCACAAA CTCTAGCAAA CAGAGACCGC
    TGTCGAGTAG CCCAATTAGC CTTGTATGAT GGACCATTTG CAGATTTGGG GGATGGTGGC
    TGTTATGGAG ACCTTTACTG CAAAGCATTA AAAACTTACA ACATGCTGTG CTTTGGTATA
    TACCGACTCC GAGATGCCCA CCTCAGCACA CCCAGCCAGT GTACCAAAAG GTACGTTATC
    ACCAACCCTC CATATGAGTT TGAACTGGTT CCCACAGATC TCATCTTTTG CCTGATGCAG
    TTCGACCATA ATGCCAGCCA GTCCCGAGCT AGCCTGTCTC ACTCTTCACA CTCCTCGCAC
    TCATCTAGCA AAAAAAGTTC ATCTGTTACC TCCATTCTAC ACACAGCCTC AGCCAACCGT
    CAGAACAGAG TCAAGGCTCG AGATTCCCGT GACAAACAAA AGACGAATAG AATGGGCCAA
    GCAGAAAAGA AATGGTATAC AGATGAAACG GAAAACACTT ATCCCAGAAA CATTCAGATT
    AAGCCAATGA GCACACATAT GGCAAATCAG ATTAATCAAT ACAAATCAAC AAGCAGCTTG
    ATACCGCCAA TACGAGAGGT CGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822393.1
    CDS1499..5194
    Translation

    Target ORF information:

    RefSeq Version XM_012966939.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966939.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GCTAAGCCTG GCAAACTGCT GCCTTTGATT TCATTCAGTC AGGAAAAAAA CAGTGGGACA
    CACATACCAA TTATTACAGA GTTGGTGAAC GACTCAAATG TTCAGTTCTT GGACCAGGAT
    GATGATGATG ACCCAGATAC AGAGTTATAC TTGACTCAGC CCTTCGCCTG TGGGACCGCC
    TTTGCTGTCA GTGTGTTAGA CTCCCTTATG AGTGCAACAT ACTTCAATGA TAACATTCTG
    ACTCTAATCA GAACTCTGGT GACTGGGGGA GCAACACCAG AGCTGGAAGC CCTCATTGCA
    GAAGAGAACG CTCTGCGTGG GGGTTATAGC ACCCCACAAA CTCTAGCAAA CAGAGACCGC
    TGTCGAGTAG CCCAATTAGC CTTGTATGAT GGACCATTTG CAGATTTGGG GGATGGTGGC
    TGTTATGGAG ACCTTTACTG CAAAGCATTA AAAACTTACA ACATGCTGTG CTTTGGTATA
    TACCGACTCC GAGATGCCCA CCTCAGCACA CCCAGCCAGT GTACCAAAAG GTACGTTATC
    ACCAACCCTC CATATGAGTT TGAACTGGTT CCCACAGATC TCATCTTTTG CCTGATGCAG
    TTCGACCATA ATGCCAGCCA GTCCCGAGCT AGCCTGTCTC ACTCTTCACA CTCCTCGCAC
    TCATCTAGCA AAAAAAGTTC ATCTGTTACC TCCATTCTAC ACACAGCCTC AGCCAACCGT
    CAGAACAGAG TCAAGGCTCG AGATTCCCGT GACAAACAAA AGACGAATAG AATGGGCCAA
    GCAGAAAAGA AATGGTATAC AGATGAAACG GAAAACACTT ATCCCAGAAA CATTCAGATT
    AAGCCAATGA GCACACATAT GGCAAATCAG ATTAATCAAT ACAAATCAAC AAGCAGCTTG
    ATACCGCCAA TACGAGAGGT CGAAGATGAA TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35195
    Clone ID Related Accession (Same CDS sequence) XM_012966940.3 , XM_012966940.2
    Accession Version XM_012966940.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966940.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GTGAACGACT CAAATGTTCA GTTCTTGGAC CAGGATGATG ATGATGACCC AGATACAGAG
    TTATACTTGA CTCAGCCCTT CGCCTGTGGG ACCGCCTTTG CTGTCAGTGT GTTAGACTCC
    CTTATGAGTG CAACATACTT CAATGATAAC ATTCTGACTC TAATCAGAAC TCTGGTGACT
    GGGGGAGCAA CACCAGAGCT GGAAGCCCTC ATTGCAGAAG AGAACGCTCT GCGTGGGGGT
    TATAGCACCC CACAAACTCT AGCAAACAGA GACCGCTGTC GAGTAGCCCA ATTAGCCTTG
    TATGATGGAC CATTTGCAGA TTTGGGGGAT GGTGGCTGTT ATGGAGACCT TTACTGCAAA
    GCATTAAAAA CTTACAACAT GCTGTGCTTT GGTATATACC GACTCCGAGA TGCCCACCTC
    AGCACACCCA GCCAGTGTAC CAAAAGGTAC GTTATCACCA ACCCTCCATA TGAGTTTGAA
    CTGGTTCCCA CAGATCTCAT CTTTTGCCTG ATGCAGTTCG ACCATAATGC CAGCCAGTCC
    CGAGCTAGCC TGTCTCACTC TTCACACTCC TCGCACTCAT CTAGCAAAAA AAGTTCATCT
    GTTACCTCCA TTCTACACAC AGCCTCAGCC AACCGTCAGA ACAGAGTCAA GGCTCGAGAT
    TCCCGTGACA AACAAAAGAC GAATAGAATG GGCCAAGCAG AAAAGAAATG GTATACAGAT
    GAAACGGAAA ACACTTATCC CAGAAACATT CAGATTAAGC CAATGAGCAC ACATATGGCA
    AATCAGATTA ATCAATACAA ATCAACAAGC AGCTTGATAC CGCCAATACG AGAGGTCGAA
    GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822394.1
    CDS1495..5106
    Translation

    Target ORF information:

    RefSeq Version XM_012966940.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966940.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GTGAACGACT CAAATGTTCA GTTCTTGGAC CAGGATGATG ATGATGACCC AGATACAGAG
    TTATACTTGA CTCAGCCCTT CGCCTGTGGG ACCGCCTTTG CTGTCAGTGT GTTAGACTCC
    CTTATGAGTG CAACATACTT CAATGATAAC ATTCTGACTC TAATCAGAAC TCTGGTGACT
    GGGGGAGCAA CACCAGAGCT GGAAGCCCTC ATTGCAGAAG AGAACGCTCT GCGTGGGGGT
    TATAGCACCC CACAAACTCT AGCAAACAGA GACCGCTGTC GAGTAGCCCA ATTAGCCTTG
    TATGATGGAC CATTTGCAGA TTTGGGGGAT GGTGGCTGTT ATGGAGACCT TTACTGCAAA
    GCATTAAAAA CTTACAACAT GCTGTGCTTT GGTATATACC GACTCCGAGA TGCCCACCTC
    AGCACACCCA GCCAGTGTAC CAAAAGGTAC GTTATCACCA ACCCTCCATA TGAGTTTGAA
    CTGGTTCCCA CAGATCTCAT CTTTTGCCTG ATGCAGTTCG ACCATAATGC CAGCCAGTCC
    CGAGCTAGCC TGTCTCACTC TTCACACTCC TCGCACTCAT CTAGCAAAAA AAGTTCATCT
    GTTACCTCCA TTCTACACAC AGCCTCAGCC AACCGTCAGA ACAGAGTCAA GGCTCGAGAT
    TCCCGTGACA AACAAAAGAC GAATAGAATG GGCCAAGCAG AAAAGAAATG GTATACAGAT
    GAAACGGAAA ACACTTATCC CAGAAACATT CAGATTAAGC CAATGAGCAC ACATATGGCA
    AATCAGATTA ATCAATACAA ATCAACAAGC AGCTTGATAC CGCCAATACG AGAGGTCGAA
    GATGAATGTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35197
    Clone ID Related Accession (Same CDS sequence) XM_012966942.2 , XM_012966942.3
    Accession Version XM_012966942.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3777bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966942.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCC AAGATGTCCA CCCGCAAGAG GCTGATACAG
    GCATGTTGTA TGGGGTGCTC TGAGATAGAC TGCTCTTGCA TGTCAGGCAC TTTGCGTAGT
    AACATGGGAA CCCTTGAACA AGCCTTCCCA ATTTCTCCTG TCTCTGTTAA TGATTTCTCC
    ACCAGTTTAC GTGGCTTTGA AGATGAGCAA CCATCAGCCT TGTCACCAAA AAAAAAGCAA
    CGAAATGGAG GAATGAGGCA ATCTCCAAAC TCTTCACCTA ACATGATGAG GCATGATCCC
    CTTCTTATGA CTGGAAATGA TCAAATTGAT AATATGGATT CTAGCAGTGT TAAAAGATAT
    GATTCTACTG GTATGTTCCA CTGGTGTCCA GCCAAGGAAT TGGATAAAGT GCTTCTGACA
    CGGAGTGAAG CTGCCATGAC AGTCCTCAGT GGGCATGTGG TTGTCTGCAT TTTTGGAGAC
    ATGACATCGG CACTGATTGG AGTTCGGAAT TTGGTGATGC CACTGAGAGC CAGCAATTTT
    CATTACCATG AGTTGAAACA TATAGTCTTT GTTGGGTCCC TTGATTACCT TAAAAGAGAA
    TGGGAAACAC TACACAACTT CCCAAAGGTT TCCATCTTGC CTGGCACACC ATTAAGTCGA
    GCAGATCTGA GAGCTGTCAA CATTAACCTG TGTGACATGT GCGTTATTCT GTCAGCCAAT
    CAGAATAATA TTGATGATAC ATCACTGCAG GACAAAGAAT GCATTTTGGC ATCTCTCAAC
    ATCAAATCCA TGCAGTTTGA TGACAGCATA GGGCTCTTGC AAGCTAACTC TCAAGGGTTT
    ACGCCCCCAG GAATGGAGAG ATCCTCACCT GATAATAGCC CAGTGCATGG TGTTGCAAGA
    CAGGCATCCA TAACTACAGG GGCCAACATT CCCATAATTA CAGAACTTGT GAACGACTCA
    AATGTTCAGT TCTTGGACCA GGATGATGAT GATGACCCAG ATACAGAGTT ATACTTGACT
    CAGCCCTTCG CCTGTGGGAC CGCCTTTGCT GTCAGTGTGT TAGACTCCCT TATGAGTGCA
    ACATACTTCA ATGATAACAT TCTGACTCTA ATCAGAACTC TGGTGACTGG GGGAGCAACA
    CCAGAGCTGG AAGCCCTCAT TGCAGAAGAG AACGCTCTGC GTGGGGGTTA TAGCACCCCA
    CAAACTCTAG CAAACAGAGA CCGCTGTCGA GTAGCCCAAT TAGCCTTGTA TGATGGACCA
    TTTGCAGATT TGGGGGATGG TGGCTGTTAT GGAGACCTTT ACTGCAAAGC ATTAAAAACT
    TACAACATGC TGTGCTTTGG TATATACCGA CTCCGAGATG CCCACCTCAG CACACCCAGC
    CAGTGTACCA AAAGGTACGT TATCACCAAC CCTCCATATG AGTTTGAACT GGTTCCCACA
    GATCTCATCT TTTGCCTGAT GCAGTTCGAC CATAATGCCA GCCAGTCCCG AGCTAGCCTG
    TCTCACTCTT CACACTCCTC GCACTCATCT AGCAAAAAAA GTTCATCTGT TACCTCCATT
    CTACACACAG CCTCAGCCAA CCGTCAGAAC AGAGTCAAGG CTCGAGATTC CCGTGACAAA
    CAAAAGACGA ATAGAATGGG CCAAGAAAAG AAATGGTATA CAGATGAAAC GGAAAACACT
    TATCCCAGAA ACATTCAGAT TAAGCCAATG AGCACACATA TGGCAAATCA GATTAATCAA
    TACAAATCAA CAAGCAGCTT GATACCGCCA ATACGAGAGG TCGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822396.1
    CDS1498..5274
    Translation

    Target ORF information:

    RefSeq Version XM_012966942.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966942.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACCC AAGATGTCCA CCCGCAAGAG GCTGATACAG
    GCATGTTGTA TGGGGTGCTC TGAGATAGAC TGCTCTTGCA TGTCAGGCAC TTTGCGTAGT
    AACATGGGAA CCCTTGAACA AGCCTTCCCA ATTTCTCCTG TCTCTGTTAA TGATTTCTCC
    ACCAGTTTAC GTGGCTTTGA AGATGAGCAA CCATCAGCCT TGTCACCAAA AAAAAAGCAA
    CGAAATGGAG GAATGAGGCA ATCTCCAAAC TCTTCACCTA ACATGATGAG GCATGATCCC
    CTTCTTATGA CTGGAAATGA TCAAATTGAT AATATGGATT CTAGCAGTGT TAAAAGATAT
    GATTCTACTG GTATGTTCCA CTGGTGTCCA GCCAAGGAAT TGGATAAAGT GCTTCTGACA
    CGGAGTGAAG CTGCCATGAC AGTCCTCAGT GGGCATGTGG TTGTCTGCAT TTTTGGAGAC
    ATGACATCGG CACTGATTGG AGTTCGGAAT TTGGTGATGC CACTGAGAGC CAGCAATTTT
    CATTACCATG AGTTGAAACA TATAGTCTTT GTTGGGTCCC TTGATTACCT TAAAAGAGAA
    TGGGAAACAC TACACAACTT CCCAAAGGTT TCCATCTTGC CTGGCACACC ATTAAGTCGA
    GCAGATCTGA GAGCTGTCAA CATTAACCTG TGTGACATGT GCGTTATTCT GTCAGCCAAT
    CAGAATAATA TTGATGATAC ATCACTGCAG GACAAAGAAT GCATTTTGGC ATCTCTCAAC
    ATCAAATCCA TGCAGTTTGA TGACAGCATA GGGCTCTTGC AAGCTAACTC TCAAGGGTTT
    ACGCCCCCAG GAATGGAGAG ATCCTCACCT GATAATAGCC CAGTGCATGG TGTTGCAAGA
    CAGGCATCCA TAACTACAGG GGCCAACATT CCCATAATTA CAGAACTTGT GAACGACTCA
    AATGTTCAGT TCTTGGACCA GGATGATGAT GATGACCCAG ATACAGAGTT ATACTTGACT
    CAGCCCTTCG CCTGTGGGAC CGCCTTTGCT GTCAGTGTGT TAGACTCCCT TATGAGTGCA
    ACATACTTCA ATGATAACAT TCTGACTCTA ATCAGAACTC TGGTGACTGG GGGAGCAACA
    CCAGAGCTGG AAGCCCTCAT TGCAGAAGAG AACGCTCTGC GTGGGGGTTA TAGCACCCCA
    CAAACTCTAG CAAACAGAGA CCGCTGTCGA GTAGCCCAAT TAGCCTTGTA TGATGGACCA
    TTTGCAGATT TGGGGGATGG TGGCTGTTAT GGAGACCTTT ACTGCAAAGC ATTAAAAACT
    TACAACATGC TGTGCTTTGG TATATACCGA CTCCGAGATG CCCACCTCAG CACACCCAGC
    CAGTGTACCA AAAGGTACGT TATCACCAAC CCTCCATATG AGTTTGAACT GGTTCCCACA
    GATCTCATCT TTTGCCTGAT GCAGTTCGAC CATAATGCCA GCCAGTCCCG AGCTAGCCTG
    TCTCACTCTT CACACTCCTC GCACTCATCT AGCAAAAAAA GTTCATCTGT TACCTCCATT
    CTACACACAG CCTCAGCCAA CCGTCAGAAC AGAGTCAAGG CTCGAGATTC CCGTGACAAA
    CAAAAGACGA ATAGAATGGG CCAAGAAAAG AAATGGTATA CAGATGAAAC GGAAAACACT
    TATCCCAGAA ACATTCAGAT TAAGCCAATG AGCACACATA TGGCAAATCA GATTAATCAA
    TACAAATCAA CAAGCAGCTT GATACCGCCA ATACGAGAGG TCGAAGATGA ATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa35200
    Clone ID Related Accession (Same CDS sequence) XM_012966946.2 , XM_012966946.3
    Accession Version XM_012966946.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product calcium-activated potassium channel subunit alpha-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966946.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GTGAACGACT CAAATGTTCA GTTCTTGGAC CAGGATGATG ATGATGACCC AGATACAGAG
    TTATACTTGA CTCAGCCCTT CGCCTGTGGG ACCGCCTTTG CTGTCAGTGT GTTAGACTCC
    CTTATGAGTG CAACATACTT CAATGATAAC ATTCTGACTC TAATCAGAAC TCTGGTGACT
    GGGGGAGCAA CACCAGAGCT GGAAGCCCTC ATTGCAGAAG AGAACGCTCT GCGTGGGGGT
    TATAGCACCC CACAAACTCT AGCAAACAGA GACCGCTGTC GAGTAGCCCA ATTAGCCTTG
    TATGATGGAC CATTTGCAGA TTTGGGGGAT GGTGGCTGTT ATGGAGACCT TTACTGCAAA
    GCATTAAAAA CTTACAACAT GCTGTGCTTT GGTATATACC GACTCCGAGA TGCCCACCTC
    AGCACACCCA GCCAGTGTAC CAAAAGGTAC GTTATCACCA ACCCTCCATA TGAGTTTGAA
    CTGGTTCCCA CAGATCTCAT CTTTTGCCTG ATGCAGTTCG ACCATAATGC CAGCCAGTCC
    CGAGCTAGCC TGTCTCACTC TTCACACTCC TCGCACTCAT CTAGCAAAAA AAGTTCATCT
    GTTACCTCCA TTCTACACAC AGCCTCAGCC AACCGTCAGA ACAGAGTCAA GGCTCGAGAT
    TCCCGTGACA AACAAAAGAC GAATAGAATG GGCCAAGAAA AGAAATGGTA TACAGATGAA
    ACGGAAAACA CTTATCCCAG AAACATTCAG ATTAAGCCAA TGAGCACACA TATGGCAAAT
    CAGATTAATC AATACAAATC AACAAGCAGC TTGATACCGC CAATACGAGA GGTCGAAGAT
    GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012822400.1
    CDS1495..5103
    Translation

    Target ORF information:

    RefSeq Version XM_012966946.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis potassium channel, calcium activated large conductance subfamily M alpha, member 1 (kcnma1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966946.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCAACCT GGAACGCGTC TCAAAGCATC TTTAATAGCA TGAGCAACAT CATTGAGAAC 
    CCACACTCCA AATCTCACCC CGTGATGGCG TCCAATGGGG AGTCCTTATT TATTCCAGTC
    ACTATGGAGG TGCCTTGTGA CAACCAGGGG ACACGTATGT GGTGGGCATT CCTGGCCTCT
    TCTATGGTGA CGTTTTTCGG AGGTCTTTTC ATTATCCTAG TGTGGAGGAC CTTCAAATAC
    CTGTGGACTG TATGTTGTCA CTGTGGGGGC AAAAACAAGG ATGCACAGAA AGTTGTAAAT
    GCAGCAAGCA GCCAGGTCAC GGATGGGGAC TACAAGCAAA CTGATGATAA AGAAGAAACA
    GCAGCACCAG GAGTGGTAGA AGTTGGCTGG ATGACATCAG TTAAAGACTG GGCAGGGGTT
    ATGATTTCTG CCCAGACCCT GACAGGTCGA GTGTTGGTTG TGACAGTCTT TGCTCTGAGC
    ATTGGAGCAC TAATGATATA CTTTATCGAC TCATCAAACC CTATTGAAAC TTGTCAAAAC
    TTTTACAAGG ACTTCACTCT TCAAATAGAC ATGGCCTTCA ACATCTTCTT TCTGCTATAT
    TTTGGCTTAC GGTTTATAGC TGCCAATGAC AAGCTTTGGT TTTGGCTGGA AGTGAATTCA
    GTGGTGGATT TCTTCACAGT ACCTCCTGTG TTTGTGTCAG TTTATTTAAA CAGGAGTTGG
    CTTGGGCTGA GGTTCCTCCG TGCTTTACGG CTGATACAGT TTTCAGAAAT CCTGCAATTT
    TTAAATATTT TAAAAACAAG TAATTCCATT AAGCTGGTGA ACCTGTGCTC CATATTTATC
    AGTACTTGGC TGACAGCTGC TGGCTTCATC CATTTGGTGG AGAACTCAGG AGATCCTTGG
    AGAAATTTTG AAAACTCCCA GGACCTTTCT TACTGGGAAT GTGTGTACCT GCTCATGGTT
    ACTATGTCCA CAGTGGGATA TGGAGATGTT TATGCAAAAA CAACCCTTGG TCGTCTCTTC
    ATGGTCTTCT TCATTCTTGG CGGTTTGGCC ATGTTTGCCA GCTACGTCCC CGAAATCATA
    GAGTTAATAG GAAACCGAAA GAAATATGGT GGTTCATATA GTGCAGTTAG TGGACGGAAG
    CATATTGTGG TGTGTGGTCA CATAACTTTA GAAAGCGTGT CCAACTTCTT GAAAGACTTT
    CTGCACAAGG ACCGAGATGA CGTGAATGTG GAGATCGTAT TTTTGCACAA CATCTCTCCA
    AATTTGGAGT TGGAAGCTTT ATTCAAGAGA CACTTTACTC AGGTAGAATT TTACCAGGGA
    TCTGTTCTCA ATCCACATGA CCTGGCAAGA GTTAAGATTG AGTCTGCAGA TGCCTGTCTG
    ATCCTTGCTA ACAAGTACTG TGCTGACCCT GATGCTGAGG ATGCATCTAA CATTATGAGA
    GTCATCTCCA TTAAAAATTA TCACCCTAAG ATAAGGATCA TCACTCAGAT GTTGCAGTAC
    CACAATAAGG CTCACCTACT AAATATACCT AGCTGGAATT GGAAAGAAGG AGATGATGCC
    ATCTGTCTTG CTGAGCTGAA ACTTGGGTTT ATTGCTCAAA GTTGCTTAGC TCAAGGTCTA
    TCAACCATGC TAGCAAACCT CTTTTCCATG CGTTCCTTTA TCAAGATCGA GGAAGACACG
    TGGCAAAAGT ATTATCTGGA AGGAGTGTCC AATGAAATGT ATACAGAATA TTTATCCAGT
    GCTTTTGTGG GCCTATCATT CCCTGCGGTT TGTGAGTTGT GCTTTGTGAA GTTGAAGCTA
    CTAATGATAG CTATCGAGTT CAAGTCAGAG AAAGGAGAGA GCAGGATCAT AATAAATCCG
    GGTAACCATG TGAAAATAAA AGAAGGTACC CTGGGATTTT TTATTGCCAG TGATGCCAAA
    GAAGTAAAAC GGGCCTTTTT TTACTGTAAA GCATGCCACG ATGACATCAC AGACCCAAAG
    AGGATAAAGA AATGCGCCTG CAAGCGACTT GAAGATGAGC AACCATCAGC CTTGTCACCA
    AAAAAAAAGC AACGAAATGG AGGAATGAGG CAATCTCCAA ACTCTTCACC TAACATGATG
    AGGCATGATC CCCTTCTTAT GACTGGAAAT GATCAAATTG ATAATATGGA TTCTAGCAGT
    GTTAAAAGAT ATGATTCTAC TGGTATGTTC CACTGGTGTC CAGCCAAGGA ATTGGATAAA
    GTGCTTCTGA CACGGAGTGA AGCTGCCATG ACAGTCCTCA GTGGGCATGT GGTTGTCTGC
    ATTTTTGGAG ACATGACATC GGCACTGATT GGAGTTCGGA ATTTGGTGAT GCCACTGAGA
    GCCAGCAATT TTCATTACCA TGAGTTGAAA CATATAGTCT TTGTTGGGTC CCTTGATTAC
    CTTAAAAGAG AATGGGAAAC ACTACACAAC TTCCCAAAGG TTTCCATCTT GCCTGGCACA
    CCATTAAGTC GAGCAGATCT GAGAGCTGTC AACATTAACC TGTGTGACAT GTGCGTTATT
    CTGTCAGCCA ATCAGAATAA TATTGATGAT ACATCACTGC AGGACAAAGA ATGCATTTTG
    GCATCTCTCA ACATCAAATC CATGCAGTTT GATGACAGCA TAGGGCTCTT GCAAGCTAAC
    TCTCAAGGGT TTACGCCCCC AGGAATGGAG AGATCCTCAC CTGATAATAG CCCAGTGCAT
    GGTGTTGCAA GACAGGCATC CATAACTACA GGGGCCAACA TTCCCATAAT TACAGAACTT
    GTGAACGACT CAAATGTTCA GTTCTTGGAC CAGGATGATG ATGATGACCC AGATACAGAG
    TTATACTTGA CTCAGCCCTT CGCCTGTGGG ACCGCCTTTG CTGTCAGTGT GTTAGACTCC
    CTTATGAGTG CAACATACTT CAATGATAAC ATTCTGACTC TAATCAGAAC TCTGGTGACT
    GGGGGAGCAA CACCAGAGCT GGAAGCCCTC ATTGCAGAAG AGAACGCTCT GCGTGGGGGT
    TATAGCACCC CACAAACTCT AGCAAACAGA GACCGCTGTC GAGTAGCCCA ATTAGCCTTG
    TATGATGGAC CATTTGCAGA TTTGGGGGAT GGTGGCTGTT ATGGAGACCT TTACTGCAAA
    GCATTAAAAA CTTACAACAT GCTGTGCTTT GGTATATACC GACTCCGAGA TGCCCACCTC
    AGCACACCCA GCCAGTGTAC CAAAAGGTAC GTTATCACCA ACCCTCCATA TGAGTTTGAA
    CTGGTTCCCA CAGATCTCAT CTTTTGCCTG ATGCAGTTCG ACCATAATGC CAGCCAGTCC
    CGAGCTAGCC TGTCTCACTC TTCACACTCC TCGCACTCAT CTAGCAAAAA AAGTTCATCT
    GTTACCTCCA TTCTACACAC AGCCTCAGCC AACCGTCAGA ACAGAGTCAA GGCTCGAGAT
    TCCCGTGACA AACAAAAGAC GAATAGAATG GGCCAAGAAA AGAAATGGTA TACAGATGAA
    ACGGAAAACA CTTATCCCAG AAACATTCAG ATTAAGCCAA TGAGCACACA TATGGCAAAT
    CAGATTAATC AATACAAATC AACAAGCAGC TTGATACCGC CAATACGAGA GGTCGAAGAT
    GAATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.