nckap1l cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nckap1l
Entrez Gene ID	100491829
Full Name	NCK-associated protein 1-like
General protein information	Preferred Names NCK-associated protein 1-like Names nck-associated protein 1-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002935658.4	XP_002935704.2	nck-associated protein 1-like
XM_002935658.5	XP_002935704.2	nck-associated protein 1-like

Pathways from BioSystems

Homologies

Homo sapiens (human)	NCKAP1L	NP_005328.2
Pan troglodytes (chimpanzee)	NCKAP1L	XP_509114.3
	NCKAP1L	XP_002798677.1
Bos taurus (cattle)	NCKAP1L	NP_001137348.1
Mus musculus (house mouse)	Nckap1l	NP_705725.1
Rattus norvegicus (Norway rat)	Nckap1l	NP_001101589.1
Danio rerio (zebrafish)	zgc:172352	NP_001107884.1
Gallus gallus (chicken)	NCKAP1L	NP_001026510.1
Xenopus tropicalis (tropical clawed frog)	nckap1l	XP_002935704.2
Canis lupus familiaris (dog)	NCKAP1L	XP_005636795.1

Gene Ontology

The following nckap1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nckap1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa52726	XM_002935658.5 Latest version!	Xenopus tropicalis NCK-associated protein 1-like (nckap1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa06084	XM_002935658.4 Latest version!	Xenopus tropicalis NCK-associated protein 1-like (nckap1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nckap1l ( XM_002935658.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935704.2 Xenopus tropicalis NCK-associated protein 1-like (nckap1l), mRNA.

Price & Availability↑

CloneID	OXa52726
Clone ID Related Accession (Same CDS sequence)	XM_002935658.5
Accession Version	XM_002935658.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935658.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG GGTCTGTATA TCAACACAAA CTGGCAGAGA AGCTTATGAT ACTGAATGAC 
AGAGGCAAAG GGGTCCTGAT CCGTATTTAC AACATCAAAA AGACATGTTC AGAAACAAAG 
ACAAAGCCAC CTTTTTTCAC TGAGAAGTCA ATGGAATCAT CTATTAAATA CATTAACAAG 
AAATTCCCCA ATATTGACGT GAGAAGCAGC ACGCAACACC TTGGCCCAGT TCACAAAGAG 
AAATCAGAGA TTGTGAAATG CCTATCAAGC TACTATTTGT CATTTGTGGA TGTCATGGAA 
TTTCGGGATC ATGTCTATGA GTTGCTGAAT ACCATTGATG CCTGTCAGTG CTACTTTGAC 
ATTAACATTA ACTACAGCTT TACCAAAAGT TACCTGGATT TGATAGTGAC ATACTTCTCT 
GTCATTTTGT TGATGTCCCG TATCGAAGAC AGAAAATCAC TAGTAGCTCT TTTTAATTGT 
GCATCTGAAA TGCTGCAAGG GCACAGTGAC CCAACCTATG CCAGGTTGGC CCAAATGCTA 
CTGGAATATG ACAACCCACT AAAAAAGCTA AGTGAGGAAT TTGGACCCCA TACAAAGACA 
GTGACCGAGG CTCTGATGTC TCTACGCTTT CTCTTCTGCC GGAGGACACA ACCAGCAGAC 
CAGTGGAGAG GGGCCCAGCT CTTGAGCCTT CTTAGCAATC CAGCTTCTAT GATTGACCCA 
TCTAGTTCTG ACACAATGCC ATGCGAATAT CTCTCCGTTG AGACAATGGA AAGATGGATT 
GTGATTGGAT TCTTGCTGTC TCATGGCTTT CTGAACACAG TCCCGGCCAG CATGGAGCTT 
TGGAAATTGG CTCTTCAGGG TTCCCATTGT ATCACACTCA TCCGGGATGA GATTCTCTTC 
ATACATCGTG TTACAGAGGA CTTCTTTGGC AGCATAAAAG GGTATGGGAA ACGTGTGGCA 
GATGTGAAAG AGTGCAAAGA ACACTTTATG ACACACTGTG GCCAGTTTCA TCGTGCCAGA 
CGCTTCTTTC TAAGAAGTGC TGTCAAGGAG TTGGAAGCCA TTTTGTCTGA TGAACCTGGA 
CTTTTGGGAC CCAAGGCACT TTTTGCATTT ATGGCTCTGT CCTTCTGCCG GGATGAGATT 
AATTGGCTGG TACGTCATGC TGAAAACATC ACCAAAACAA AGACACCAGA AGATTACGTG 
GATGTACAAA TTGCAGAACT GATCTTTTAC GTGGAGGCGA TTCGGGCAAT AGTAAAGAAG 
CATCTGTCTG TCATACAGAA ATACCACATT CAGTATCTGG CTCGGTTTGA TGCTTTGGTT 
CTGAGTGATG TCATTCAGAA TCTGACTGTC TGCCCAGAAG AAGAATCTGT GATCATGTCA 
TCATTTGTTA GCACCTTGTC AGCACTCAGT GTAAAACAAG TTGAAAACAA AGAAACATTT 
GATTTTTGCG CTCTGAGACT TGACTGGTTC CGGCTGCAGG CTTATACAAG CATAGCAAAA 
GCCCCACTTT CCCTGCGGGA TAACCCCGAC TTAGGCAAAG TGATGAATCT AATCATGTTT 
CACAGCTGGA TGTTGGACTC AGTGGAAGAG CTTCTTGTGG AGACATCTGA CCTCTCAGCC 
TTTTGCTTTC ATGTACGCAC ATTTGAGAAG ATGTTCTTGA TGACAATGGA AGAGCCTTCT 
GCTTTGCGTT ATTCGATTGC CTTTCCTCTG ATCTGCTCCC ACTTCTCCAG TTCCATTCAT 
GAGATGTGCC CAGAGGAGTA CCCACATCTT AAGAACTGCA GCCTAGGATT CTGCAACAAC 
TTCTTGGATG AGATGAGCAA GCACGCCAGT AACTGTGCGA TGGACATCTG TGCTGAGCAG 
TGCAACCTCA GTGAGAAGCT GCTTCCAAAG CACTGTGCTG CCACAATCAG CAAAGCCAGA 
AACAAGAGGC TAAAAAAGCC ACCTCCAAAG AGGGCAGAGC CAGAAAGGGA AAAGCCAGGC 
GCAGAAAGCC AAAGACGCGA CCGCAGTGTT ACTACAAACT TGGACAAATT GCATCTGACT 
CTGTGTGAAC TCTCCATGGC ACTGAACCAT GTGCGAAGTA TTCGTGTGTT TGAGCATGTG 
GTCACCCCAG CAGAATACCT GAGTTCCCAC CTTGAGAACC GATTTGGGAG GGCTTTAGTT 
AATATGGTGA ACTTCAAACA GACAACGAGA GAAATTCTGC GCCCATCTGA GGTCCTGCAT 
GGGATGCGTT CTTACCTAAG TACCCTTAAG TCCATTGGTC ACTTGGTAAA CATAGATGTG 
ACGCGTATTA TACGGAGCAG CTTGTTACAG CAGACACAGC CAACTGATGC CAATGGGGAA 
CCAACCATCA CTGCTTTGTA TACTAACTGG TACCTGGAGT GTCTTCTCCG CCAAGCCAGC 
GCAGGGTTTA TTATCTATTC ACCAGCAAGG AGAACCTTCA TGGACCTACC GACTGAGATG 
AGTGGTGAAC AAGGCCTTAA TGCCGAGGAA TACTCCGACG TGTCAGAAAT GAAAGCTTTA 
AGTGAGCTGA TTGGCCCTTA TGGAATGAAG TTTTTGAGCG AAAATCTGAT GTGGCATGTA 
ACCTCCCAAA TTGTGGAACT TAAGAAACTG GTTATAGAGA ACATGGATGT GCTGGTACAA 
ATCCGCTCGA ACTTCAGCAA TTCAGAACTT ATGCCGTCCC TTCTAGCCAA GCTCTCCTCT 
CCTGAGAATT TGCTAAAAAG AATGACAATT ATTGGTGTTA TCTTATCTTT TCGGGCTTTG 
ACACAGGAAG GTCTCAGAGA GGTGTTTGCT CATCATTGCC CATTTATAAT GAGTCCCATT 
GAATCGCTGA AGGACCTGGT GAACCCTGAT ATTGATATTC AGGTGACTCT GAGCATTTTT 
GAACTGGCAA CTGCGGCGGG TGTGCCCTGT GATGTAGACC CAAGCCTGGT TGCCGCTCTC 
TCAAACATTA AAACAGACAG TGCTTCCCTA GAGGAAGAGT ACAAGGTAGC TTGCCTGCTG 
CAGGTCTTCC TAGCGGTATC TCTTCCCCTG CTGGCCTCAG ATCCAATGTC ATTTTACAGC 
ATTGAAAATG ATGGTTACAA TAACAACATT CACTGCTTGG CCAAAGCTAT TATCCAGGTG 
TCTGCTGCCC TCTTTACTCT CTACAATAAG AACATTGAGA CCCACCTAAA GGAATTCCTG 
TTGATGGCAT CAGGGAGCCT GCTACAACTT GGGGAGGAAA CTGATAAATC AAAAGCCAGA 
AATCGAGAGT CGATACTGAT TCTGCTGCAT CTTATTGTAG AAGAGTCTTC ATTCCTGACA 
GTGGACATGC TGGAATCCTG CTTCCCTTAT GTTCTGCTCC GCAATGCCTA CCATGAAGTC 
CACAGAGCTT TTCCTACTGT TACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935704.2
CDS	185..3571
Translation	MSLGSVYQHKLAEKLMILNDRGKGVLIRIYNIKKTCSETKTKPPFFTEKSMESSIKYINKKFPNIDVRSSTQHLGPVHKEKSEIVKCLSSYYLSFVDVMEFRDHVYELLNTIDACQCYFDININYSFTKSYLDLIVTYFSVILLMSRIEDRKSLVALFNCASEMLQGHSDPTYARLAQMLLEYDNPLKKLSEEFGPHTKTVTEALMSLRFLFCRRTQPADQWRGAQLLSLLSNPASMIDPSSSDTMPCEYLSVETMERWIVIGFLLSHGFLNTVPASMELWKLALQGSHCITLIRDEILFIHRVTEDFFGSIKGYGKRVADVKECKEHFMTHCGQFHRARRFFLRSAVKELEAILSDEPGLLGPKALFAFMALSFCRDEINWLVRHAENITKTKTPEDYVDVQIAELIFYVEAIRAIVKKHLSVIQKYHIQYLARFDALVLSDVIQNLTVCPEEESVIMSSFVSTLSALSVKQVENKETFDFCALRLDWFRLQAYTSIAKAPLSLRDNPDLGKVMNLIMFHSWMLDSVEELLVETSDLSAFCFHVRTFEKMFLMTMEEPSALRYSIAFPLICSHFSSSIHEMCPEEYPHLKNCSLGFCNNFLDEMSKHASNCAMDICAEQCNLSEKLLPKHCAATISKARNKRLKKPPPKRAEPEREKPGAESQRRDRSVTTNLDKLHLTLCELSMALNHVRSIRVFEHVVTPAEYLSSHLENRFGRALVNMVNFKQTTREILRPSEVLHGMRSYLSTLKSIGHLVNIDVTRIIRSSLLQQTQPTDANGEPTITALYTNWYLECLLRQASAGFIIYSPARRTFMDLPTEMSGEQGLNAEEYSDVSEMKALSELIGPYGMKFLSENLMWHVTSQIVELKKLVIENMDVLVQIRSNFSNSELMPSLLAKLSSPENLLKRMTIIGVILSFRALTQEGLREVFAHHCPFIMSPIESLKDLVNPDIDIQVTLSIFELATAAGVPCDVDPSLVAALSNIKTDSASLEEEYKVACLLQVFLAVSLPLLASDPMSFYSIENDGYNNNIHCLAKAIIQVSAALFTLYNKNIETHLKEFLLMASGSLLQLGEETDKSKARNRESILILLHLIVEESSFLTVDMLESCFPYVLLRNAYHEVHRAFPTVT

Target ORF information:

RefSeq Version	XM_002935658.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 1-like (nckap1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935658.5

ORF Insert Sequence:

ATGTCTCTGG GGTCTGTATA TCAACACAAA CTGGCAGAGA AGCTTATGAT ACTGAATGAC 
AGAGGCAAAG GGGTCCTGAT CCGTATTTAC AACATCAAAA AGACATGTTC AGAAACAAAG 
ACAAAGCCAC CTTTTTTCAC TGAGAAGTCA ATGGAATCAT CTATTAAATA CATTAACAAG 
AAATTCCCCA ATATTGACGT GAGAAGCAGC ACGCAACACC TTGGCCCAGT TCACAAAGAG 
AAATCAGAGA TTGTGAAATG CCTATCAAGC TACTATTTGT CATTTGTGGA TGTCATGGAA 
TTTCGGGATC ATGTCTATGA GTTGCTGAAT ACCATTGATG CCTGTCAGTG CTACTTTGAC 
ATTAACATTA ACTACAGCTT TACCAAAAGT TACCTGGATT TGATAGTGAC ATACTTCTCT 
GTCATTTTGT TGATGTCCCG TATCGAAGAC AGAAAATCAC TAGTAGCTCT TTTTAATTGT 
GCATCTGAAA TGCTGCAAGG GCACAGTGAC CCAACCTATG CCAGGTTGGC CCAAATGCTA 
CTGGAATATG ACAACCCACT AAAAAAGCTA AGTGAGGAAT TTGGACCCCA TACAAAGACA 
GTGACCGAGG CTCTGATGTC TCTACGCTTT CTCTTCTGCC GGAGGACACA ACCAGCAGAC 
CAGTGGAGAG GGGCCCAGCT CTTGAGCCTT CTTAGCAATC CAGCTTCTAT GATTGACCCA 
TCTAGTTCTG ACACAATGCC ATGCGAATAT CTCTCCGTTG AGACAATGGA AAGATGGATT 
GTGATTGGAT TCTTGCTGTC TCATGGCTTT CTGAACACAG TCCCGGCCAG CATGGAGCTT 
TGGAAATTGG CTCTTCAGGG TTCCCATTGT ATCACACTCA TCCGGGATGA GATTCTCTTC 
ATACATCGTG TTACAGAGGA CTTCTTTGGC AGCATAAAAG GGTATGGGAA ACGTGTGGCA 
GATGTGAAAG AGTGCAAAGA ACACTTTATG ACACACTGTG GCCAGTTTCA TCGTGCCAGA 
CGCTTCTTTC TAAGAAGTGC TGTCAAGGAG TTGGAAGCCA TTTTGTCTGA TGAACCTGGA 
CTTTTGGGAC CCAAGGCACT TTTTGCATTT ATGGCTCTGT CCTTCTGCCG GGATGAGATT 
AATTGGCTGG TACGTCATGC TGAAAACATC ACCAAAACAA AGACACCAGA AGATTACGTG 
GATGTACAAA TTGCAGAACT GATCTTTTAC GTGGAGGCGA TTCGGGCAAT AGTAAAGAAG 
CATCTGTCTG TCATACAGAA ATACCACATT CAGTATCTGG CTCGGTTTGA TGCTTTGGTT 
CTGAGTGATG TCATTCAGAA TCTGACTGTC TGCCCAGAAG AAGAATCTGT GATCATGTCA 
TCATTTGTTA GCACCTTGTC AGCACTCAGT GTAAAACAAG TTGAAAACAA AGAAACATTT 
GATTTTTGCG CTCTGAGACT TGACTGGTTC CGGCTGCAGG CTTATACAAG CATAGCAAAA 
GCCCCACTTT CCCTGCGGGA TAACCCCGAC TTAGGCAAAG TGATGAATCT AATCATGTTT 
CACAGCTGGA TGTTGGACTC AGTGGAAGAG CTTCTTGTGG AGACATCTGA CCTCTCAGCC 
TTTTGCTTTC ATGTACGCAC ATTTGAGAAG ATGTTCTTGA TGACAATGGA AGAGCCTTCT 
GCTTTGCGTT ATTCGATTGC CTTTCCTCTG ATCTGCTCCC ACTTCTCCAG TTCCATTCAT 
GAGATGTGCC CAGAGGAGTA CCCACATCTT AAGAACTGCA GCCTAGGATT CTGCAACAAC 
TTCTTGGATG AGATGAGCAA GCACGCCAGT AACTGTGCGA TGGACATCTG TGCTGAGCAG 
TGCAACCTCA GTGAGAAGCT GCTTCCAAAG CACTGTGCTG CCACAATCAG CAAAGCCAGA 
AACAAGAGGC TAAAAAAGCC ACCTCCAAAG AGGGCAGAGC CAGAAAGGGA AAAGCCAGGC 
GCAGAAAGCC AAAGACGCGA CCGCAGTGTT ACTACAAACT TGGACAAATT GCATCTGACT 
CTGTGTGAAC TCTCCATGGC ACTGAACCAT GTGCGAAGTA TTCGTGTGTT TGAGCATGTG 
GTCACCCCAG CAGAATACCT GAGTTCCCAC CTTGAGAACC GATTTGGGAG GGCTTTAGTT 
AATATGGTGA ACTTCAAACA GACAACGAGA GAAATTCTGC GCCCATCTGA GGTCCTGCAT 
GGGATGCGTT CTTACCTAAG TACCCTTAAG TCCATTGGTC ACTTGGTAAA CATAGATGTG 
ACGCGTATTA TACGGAGCAG CTTGTTACAG CAGACACAGC CAACTGATGC CAATGGGGAA 
CCAACCATCA CTGCTTTGTA TACTAACTGG TACCTGGAGT GTCTTCTCCG CCAAGCCAGC 
GCAGGGTTTA TTATCTATTC ACCAGCAAGG AGAACCTTCA TGGACCTACC GACTGAGATG 
AGTGGTGAAC AAGGCCTTAA TGCCGAGGAA TACTCCGACG TGTCAGAAAT GAAAGCTTTA 
AGTGAGCTGA TTGGCCCTTA TGGAATGAAG TTTTTGAGCG AAAATCTGAT GTGGCATGTA 
ACCTCCCAAA TTGTGGAACT TAAGAAACTG GTTATAGAGA ACATGGATGT GCTGGTACAA 
ATCCGCTCGA ACTTCAGCAA TTCAGAACTT ATGCCGTCCC TTCTAGCCAA GCTCTCCTCT 
CCTGAGAATT TGCTAAAAAG AATGACAATT ATTGGTGTTA TCTTATCTTT TCGGGCTTTG 
ACACAGGAAG GTCTCAGAGA GGTGTTTGCT CATCATTGCC CATTTATAAT GAGTCCCATT 
GAATCGCTGA AGGACCTGGT GAACCCTGAT ATTGATATTC AGGTGACTCT GAGCATTTTT 
GAACTGGCAA CTGCGGCGGG TGTGCCCTGT GATGTAGACC CAAGCCTGGT TGCCGCTCTC 
TCAAACATTA AAACAGACAG TGCTTCCCTA GAGGAAGAGT ACAAGGTAGC TTGCCTGCTG 
CAGGTCTTCC TAGCGGTATC TCTTCCCCTG CTGGCCTCAG ATCCAATGTC ATTTTACAGC 
ATTGAAAATG ATGGTTACAA TAACAACATT CACTGCTTGG CCAAAGCTAT TATCCAGGTG 
TCTGCTGCCC TCTTTACTCT CTACAATAAG AACATTGAGA CCCACCTAAA GGAATTCCTG 
TTGATGGCAT CAGGGAGCCT GCTACAACTT GGGGAGGAAA CTGATAAATC AAAAGCCAGA 
AATCGAGAGT CGATACTGAT TCTGCTGCAT CTTATTGTAG AAGAGTCTTC ATTCCTGACA 
GTGGACATGC TGGAATCCTG CTTCCCTTAT GTTCTGCTCC GCAATGCCTA CCATGAAGTC 
CACAGAGCTT TTCCTACTGT TACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap1l ( XM_002935658.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935704.2 Xenopus tropicalis NCK-associated protein 1-like (nckap1l), mRNA.

Price & Availability↑

CloneID	OXa06084
Clone ID Related Accession (Same CDS sequence)	XM_002935658.4
Accession Version	XM_002935658.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935658.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG GGTCTGTATA TCAACACAAA CTGGCAGAGA AGCTTATGAT ACTGAATGAC 
AGAGGCAAAG GGGTCCTGAT CCGTATTTAC AACATCAAAA AGACATGTTC AGAAACAAAG 
ACAAAGCCAC CTTTTTTCAC TGAGAAGTCA ATGGAATCAT CTATTAAATA CATTAACAAG 
AAATTCCCCA ATATTGACGT GAGAAGCAGC ACGCAACACC TTGGCCCAGT TCACAAAGAG 
AAATCAGAGA TTGTGAAATG CCTATCAAGC TACTATTTGT CATTTGTGGA TGTCATGGAA 
TTTCGGGATC ATGTCTATGA GTTGCTGAAT ACCATTGATG CCTGTCAGTG CTACTTTGAC 
ATTAACATTA ACTACAGCTT TACCAAAAGT TACCTGGATT TGATAGTGAC ATACTTCTCT 
GTCATTTTGT TGATGTCCCG TATCGAAGAC AGAAAATCAC TAGTAGCTCT TTTTAATTGT 
GCATCTGAAA TGCTGCAAGG GCACAGTGAC CCAACCTATG CCAGGTTGGC CCAAATGCTA 
CTGGAATATG ACAACCCACT AAAAAAGCTA AGTGAGGAAT TTGGACCCCA TACAAAGACA 
GTGACCGAGG CTCTGATGTC TCTACGCTTT CTCTTCTGCC GGAGGACACA ACCAGCAGAC 
CAGTGGAGAG GGGCCCAGCT CTTGAGCCTT CTTAGCAATC CAGCTTCTAT GATTGACCCA 
TCTAGTTCTG ACACAATGCC ATGCGAATAT CTCTCCGTTG AGACAATGGA AAGATGGATT 
GTGATTGGAT TCTTGCTGTC TCATGGCTTT CTGAACACAG TCCCGGCCAG CATGGAGCTT 
TGGAAATTGG CTCTTCAGGG TTCCCATTGT ATCACACTCA TCCGGGATGA GATTCTCTTC 
ATACATCGTG TTACAGAGGA CTTCTTTGGC AGCATAAAAG GGTATGGGAA ACGTGTGGCA 
GATGTGAAAG AGTGCAAAGA ACACTTTATG ACACACTGTG GCCAGTTTCA TCGTGCCAGA 
CGCTTCTTTC TAAGAAGTGC TGTCAAGGAG TTGGAAGCCA TTTTGTCTGA TGAACCTGGA 
CTTTTGGGAC CCAAGGCACT TTTTGCATTT ATGGCTCTGT CCTTCTGCCG GGATGAGATT 
AATTGGCTGG TACGTCATGC TGAAAACATC ACCAAAACAA AGACACCAGA AGATTACGTG 
GATGTACAAA TTGCAGAACT GATCTTTTAC GTGGAGGCGA TTCGGGCAAT AGTAAAGAAG 
CATCTGTCTG TCATACAGAA ATACCACATT CAGTATCTGG CTCGGTTTGA TGCTTTGGTT 
CTGAGTGATG TCATTCAGAA TCTGACTGTC TGCCCAGAAG AAGAATCTGT GATCATGTCA 
TCATTTGTTA GCACCTTGTC AGCACTCAGT GTAAAACAAG TTGAAAACAA AGAAACATTT 
GATTTTTGCG CTCTGAGACT TGACTGGTTC CGGCTGCAGG CTTATACAAG CATAGCAAAA 
GCCCCACTTT CCCTGCGGGA TAACCCCGAC TTAGGCAAAG TGATGAATCT AATCATGTTT 
CACAGCTGGA TGTTGGACTC AGTGGAAGAG CTTCTTGTGG AGACATCTGA CCTCTCAGCC 
TTTTGCTTTC ATGTACGCAC ATTTGAGAAG ATGTTCTTGA TGACAATGGA AGAGCCTTCT 
GCTTTGCGTT ATTCGATTGC CTTTCCTCTG ATCTGCTCCC ACTTCTCCAG TTCCATTCAT 
GAGATGTGCC CAGAGGAGTA CCCACATCTT AAGAACTGCA GCCTAGGATT CTGCAACAAC 
TTCTTGGATG AGATGAGCAA GCACGCCAGT AACTGTGCGA TGGACATCTG TGCTGAGCAG 
TGCAACCTCA GTGAGAAGCT GCTTCCAAAG CACTGTGCTG CCACAATCAG CAAAGCCAGA 
AACAAGAGGC TAAAAAAGCC ACCTCCAAAG AGGGCAGAGC CAGAAAGGGA AAAGCCAGGC 
GCAGAAAGCC AAAGACGCGA CCGCAGTGTT ACTACAAACT TGGACAAATT GCATCTGACT 
CTGTGTGAAC TCTCCATGGC ACTGAACCAT GTGCGAAGTA TTCGTGTGTT TGAGCATGTG 
GTCACCCCAG CAGAATACCT GAGTTCCCAC CTTGAGAACC GATTTGGGAG GGCTTTAGTT 
AATATGGTGA ACTTCAAACA GACAACGAGA GAAATTCTGC GCCCATCTGA GGTCCTGCAT 
GGGATGCGTT CTTACCTAAG TACCCTTAAG TCCATTGGTC ACTTGGTAAA CATAGATGTG 
ACGCGTATTA TACGGAGCAG CTTGTTACAG CAGACACAGC CAACTGATGC CAATGGGGAA 
CCAACCATCA CTGCTTTGTA TACTAACTGG TACCTGGAGT GTCTTCTCCG CCAAGCCAGC 
GCAGGGTTTA TTATCTATTC ACCAGCAAGG AGAACCTTCA TGGACCTACC GACTGAGATG 
AGTGGTGAAC AAGGCCTTAA TGCCGAGGAA TACTCCGACG TGTCAGAAAT GAAAGCTTTA 
AGTGAGCTGA TTGGCCCTTA TGGAATGAAG TTTTTGAGCG AAAATCTGAT GTGGCATGTA 
ACCTCCCAAA TTGTGGAACT TAAGAAACTG GTTATAGAGA ACATGGATGT GCTGGTACAA 
ATCCGCTCGA ACTTCAGCAA TTCAGAACTT ATGCCGTCCC TTCTAGCCAA GCTCTCCTCT 
CCTGAGAATT TGCTAAAAAG AATGACAATT ATTGGTGTTA TCTTATCTTT TCGGGCTTTG 
ACACAGGAAG GTCTCAGAGA GGTGTTTGCT CATCATTGCC CATTTATAAT GAGTCCCATT 
GAATCGCTGA AGGACCTGGT GAACCCTGAT ATTGATATTC AGGTGACTCT GAGCATTTTT 
GAACTGGCAA CTGCGGCGGG TGTGCCCTGT GATGTAGACC CAAGCCTGGT TGCTGCTCTC 
TCAAACATTA AAACAGACAG TGCTTCCCTA GAGGAAGAGT ACAAGGTAGC TTGCCTGCTG 
CAGGTCTTCC TAGCGGTATC TCTTCCCCTG CTGGCCTCAG ATCCAATGTC ATTTTACAGC 
ATTGAAAATG ATGGTTACAA TAACAACATT CACTGCTTGG CCAAAGCGAT TATCCAGGTG 
TCTGCTGCCC TCTTTACTCT CTACAATAAG AACATTGAGA CCCACCTAAA GGAATTCCTG 
TTGATGGCAT CAGGGAGCCT GCTACAACTT GGGGAGGAAA CTGATAAATC AAAAGCCAGA 
AATCGAGAGT CGATACTGAT TCTGCTGCAT CTTATTGTAG AAGAGTCTTC ATTCCTGACA 
GTGGACATGC TGGAATCCTG CTTCCCTTAT GTTCTGCTCC GCAATGCCTA CCATGAAGTC 
CACAGAGCTT TTCCTACTGT TACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935704.2
CDS	190..3576
Translation	MSLGSVYQHKLAEKLMILNDRGKGVLIRIYNIKKTCSETKTKPPFFTEKSMESSIKYINKKFPNIDVRSSTQHLGPVHKEKSEIVKCLSSYYLSFVDVMEFRDHVYELLNTIDACQCYFDININYSFTKSYLDLIVTYFSVILLMSRIEDRKSLVALFNCASEMLQGHSDPTYARLAQMLLEYDNPLKKLSEEFGPHTKTVTEALMSLRFLFCRRTQPADQWRGAQLLSLLSNPASMIDPSSSDTMPCEYLSVETMERWIVIGFLLSHGFLNTVPASMELWKLALQGSHCITLIRDEILFIHRVTEDFFGSIKGYGKRVADVKECKEHFMTHCGQFHRARRFFLRSAVKELEAILSDEPGLLGPKALFAFMALSFCRDEINWLVRHAENITKTKTPEDYVDVQIAELIFYVEAIRAIVKKHLSVIQKYHIQYLARFDALVLSDVIQNLTVCPEEESVIMSSFVSTLSALSVKQVENKETFDFCALRLDWFRLQAYTSIAKAPLSLRDNPDLGKVMNLIMFHSWMLDSVEELLVETSDLSAFCFHVRTFEKMFLMTMEEPSALRYSIAFPLICSHFSSSIHEMCPEEYPHLKNCSLGFCNNFLDEMSKHASNCAMDICAEQCNLSEKLLPKHCAATISKARNKRLKKPPPKRAEPEREKPGAESQRRDRSVTTNLDKLHLTLCELSMALNHVRSIRVFEHVVTPAEYLSSHLENRFGRALVNMVNFKQTTREILRPSEVLHGMRSYLSTLKSIGHLVNIDVTRIIRSSLLQQTQPTDANGEPTITALYTNWYLECLLRQASAGFIIYSPARRTFMDLPTEMSGEQGLNAEEYSDVSEMKALSELIGPYGMKFLSENLMWHVTSQIVELKKLVIENMDVLVQIRSNFSNSELMPSLLAKLSSPENLLKRMTIIGVILSFRALTQEGLREVFAHHCPFIMSPIESLKDLVNPDIDIQVTLSIFELATAAGVPCDVDPSLVAALSNIKTDSASLEEEYKVACLLQVFLAVSLPLLASDPMSFYSIENDGYNNNIHCLAKAIIQVSAALFTLYNKNIETHLKEFLLMASGSLLQLGEETDKSKARNRESILILLHLIVEESSFLTVDMLESCFPYVLLRNAYHEVHRAFPTVT

Target ORF information:

RefSeq Version	XM_002935658.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 1-like (nckap1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935658.4

ORF Insert Sequence:

ATGTCTCTGG GGTCTGTATA TCAACACAAA CTGGCAGAGA AGCTTATGAT ACTGAATGAC 
AGAGGCAAAG GGGTCCTGAT CCGTATTTAC AACATCAAAA AGACATGTTC AGAAACAAAG 
ACAAAGCCAC CTTTTTTCAC TGAGAAGTCA ATGGAATCAT CTATTAAATA CATTAACAAG 
AAATTCCCCA ATATTGACGT GAGAAGCAGC ACGCAACACC TTGGCCCAGT TCACAAAGAG 
AAATCAGAGA TTGTGAAATG CCTATCAAGC TACTATTTGT CATTTGTGGA TGTCATGGAA 
TTTCGGGATC ATGTCTATGA GTTGCTGAAT ACCATTGATG CCTGTCAGTG CTACTTTGAC 
ATTAACATTA ACTACAGCTT TACCAAAAGT TACCTGGATT TGATAGTGAC ATACTTCTCT 
GTCATTTTGT TGATGTCCCG TATCGAAGAC AGAAAATCAC TAGTAGCTCT TTTTAATTGT 
GCATCTGAAA TGCTGCAAGG GCACAGTGAC CCAACCTATG CCAGGTTGGC CCAAATGCTA 
CTGGAATATG ACAACCCACT AAAAAAGCTA AGTGAGGAAT TTGGACCCCA TACAAAGACA 
GTGACCGAGG CTCTGATGTC TCTACGCTTT CTCTTCTGCC GGAGGACACA ACCAGCAGAC 
CAGTGGAGAG GGGCCCAGCT CTTGAGCCTT CTTAGCAATC CAGCTTCTAT GATTGACCCA 
TCTAGTTCTG ACACAATGCC ATGCGAATAT CTCTCCGTTG AGACAATGGA AAGATGGATT 
GTGATTGGAT TCTTGCTGTC TCATGGCTTT CTGAACACAG TCCCGGCCAG CATGGAGCTT 
TGGAAATTGG CTCTTCAGGG TTCCCATTGT ATCACACTCA TCCGGGATGA GATTCTCTTC 
ATACATCGTG TTACAGAGGA CTTCTTTGGC AGCATAAAAG GGTATGGGAA ACGTGTGGCA 
GATGTGAAAG AGTGCAAAGA ACACTTTATG ACACACTGTG GCCAGTTTCA TCGTGCCAGA 
CGCTTCTTTC TAAGAAGTGC TGTCAAGGAG TTGGAAGCCA TTTTGTCTGA TGAACCTGGA 
CTTTTGGGAC CCAAGGCACT TTTTGCATTT ATGGCTCTGT CCTTCTGCCG GGATGAGATT 
AATTGGCTGG TACGTCATGC TGAAAACATC ACCAAAACAA AGACACCAGA AGATTACGTG 
GATGTACAAA TTGCAGAACT GATCTTTTAC GTGGAGGCGA TTCGGGCAAT AGTAAAGAAG 
CATCTGTCTG TCATACAGAA ATACCACATT CAGTATCTGG CTCGGTTTGA TGCTTTGGTT 
CTGAGTGATG TCATTCAGAA TCTGACTGTC TGCCCAGAAG AAGAATCTGT GATCATGTCA 
TCATTTGTTA GCACCTTGTC AGCACTCAGT GTAAAACAAG TTGAAAACAA AGAAACATTT 
GATTTTTGCG CTCTGAGACT TGACTGGTTC CGGCTGCAGG CTTATACAAG CATAGCAAAA 
GCCCCACTTT CCCTGCGGGA TAACCCCGAC TTAGGCAAAG TGATGAATCT AATCATGTTT 
CACAGCTGGA TGTTGGACTC AGTGGAAGAG CTTCTTGTGG AGACATCTGA CCTCTCAGCC 
TTTTGCTTTC ATGTACGCAC ATTTGAGAAG ATGTTCTTGA TGACAATGGA AGAGCCTTCT 
GCTTTGCGTT ATTCGATTGC CTTTCCTCTG ATCTGCTCCC ACTTCTCCAG TTCCATTCAT 
GAGATGTGCC CAGAGGAGTA CCCACATCTT AAGAACTGCA GCCTAGGATT CTGCAACAAC 
TTCTTGGATG AGATGAGCAA GCACGCCAGT AACTGTGCGA TGGACATCTG TGCTGAGCAG 
TGCAACCTCA GTGAGAAGCT GCTTCCAAAG CACTGTGCTG CCACAATCAG CAAAGCCAGA 
AACAAGAGGC TAAAAAAGCC ACCTCCAAAG AGGGCAGAGC CAGAAAGGGA AAAGCCAGGC 
GCAGAAAGCC AAAGACGCGA CCGCAGTGTT ACTACAAACT TGGACAAATT GCATCTGACT 
CTGTGTGAAC TCTCCATGGC ACTGAACCAT GTGCGAAGTA TTCGTGTGTT TGAGCATGTG 
GTCACCCCAG CAGAATACCT GAGTTCCCAC CTTGAGAACC GATTTGGGAG GGCTTTAGTT 
AATATGGTGA ACTTCAAACA GACAACGAGA GAAATTCTGC GCCCATCTGA GGTCCTGCAT 
GGGATGCGTT CTTACCTAAG TACCCTTAAG TCCATTGGTC ACTTGGTAAA CATAGATGTG 
ACGCGTATTA TACGGAGCAG CTTGTTACAG CAGACACAGC CAACTGATGC CAATGGGGAA 
CCAACCATCA CTGCTTTGTA TACTAACTGG TACCTGGAGT GTCTTCTCCG CCAAGCCAGC 
GCAGGGTTTA TTATCTATTC ACCAGCAAGG AGAACCTTCA TGGACCTACC GACTGAGATG 
AGTGGTGAAC AAGGCCTTAA TGCCGAGGAA TACTCCGACG TGTCAGAAAT GAAAGCTTTA 
AGTGAGCTGA TTGGCCCTTA TGGAATGAAG TTTTTGAGCG AAAATCTGAT GTGGCATGTA 
ACCTCCCAAA TTGTGGAACT TAAGAAACTG GTTATAGAGA ACATGGATGT GCTGGTACAA 
ATCCGCTCGA ACTTCAGCAA TTCAGAACTT ATGCCGTCCC TTCTAGCCAA GCTCTCCTCT 
CCTGAGAATT TGCTAAAAAG AATGACAATT ATTGGTGTTA TCTTATCTTT TCGGGCTTTG 
ACACAGGAAG GTCTCAGAGA GGTGTTTGCT CATCATTGCC CATTTATAAT GAGTCCCATT 
GAATCGCTGA AGGACCTGGT GAACCCTGAT ATTGATATTC AGGTGACTCT GAGCATTTTT 
GAACTGGCAA CTGCGGCGGG TGTGCCCTGT GATGTAGACC CAAGCCTGGT TGCTGCTCTC 
TCAAACATTA AAACAGACAG TGCTTCCCTA GAGGAAGAGT ACAAGGTAGC TTGCCTGCTG 
CAGGTCTTCC TAGCGGTATC TCTTCCCCTG CTGGCCTCAG ATCCAATGTC ATTTTACAGC 
ATTGAAAATG ATGGTTACAA TAACAACATT CACTGCTTGG CCAAAGCGAT TATCCAGGTG 
TCTGCTGCCC TCTTTACTCT CTACAATAAG AACATTGAGA CCCACCTAAA GGAATTCCTG 
TTGATGGCAT CAGGGAGCCT GCTACAACTT GGGGAGGAAA CTGATAAATC AAAAGCCAGA 
AATCGAGAGT CGATACTGAT TCTGCTGCAT CTTATTGTAG AAGAGTCTTC ATTCCTGACA 
GTGGACATGC TGGAATCCTG CTTCCCTTAT GTTCTGCTCC GCAATGCCTA CCATGAAGTC 
CACAGAGCTT TTCCTACTGT TACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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nckap1l cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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