ube4b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ube4b
Entrez Gene ID	100491174
Full Name	ubiquitination factor E4B
General protein information	Preferred Names ubiquitination factor E4B Names ubiquitin conjugation factor E4 B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018095832.1	XP_017951321.1	ubiquitin conjugation factor E4 B isoform X1
XM_031906349.1	XP_031762209.1	ubiquitin conjugation factor E4 B isoform X1
XM_018095832.2	XP_017951321.1	ubiquitin conjugation factor E4 B isoform X2
XM_004916027.4	XP_004916084.1	ubiquitin conjugation factor E4 B isoform X4
XM_018095833.1	XP_017951322.1	ubiquitin conjugation factor E4 B isoform X2
XM_004916027.3	XP_004916084.1	ubiquitin conjugation factor E4 B isoform X3
XM_018095833.2	XP_017951322.1	ubiquitin conjugation factor E4 B isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE4B	XP_001161561.1
Kluyveromyces lactis	KLLA0F05269g	XP_455319.1
Eremothecium gossypii	AGOS_ADR096C	NP_984192.1
Neurospora crassa	NCU03357	XP_956466.1
Caenorhabditis elegans (roundworm)	ufd-2	NP_495691.1
Schizosaccharomyces pombe (fission yeast)	ufd2	NP_593630.1
Rattus norvegicus (Norway rat)	Ube4b	NP_001258127.1
Drosophila melanogaster (fruit fly)	CG9934	NP_609597.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007870	XP_317622.4
Magnaporthe oryzae (rice blast fungus)	MGG_03255	XP_003716719.1
Arabidopsis thaliana (thale cress)	AT5G15400	NP_568313.2
Mus musculus (house mouse)	Ube4b	NP_071305.2
Danio rerio (zebrafish)	ube4b	NP_919343.1
Xenopus tropicalis (tropical clawed frog)	ube4b	XP_004916084.1
Homo sapiens (human)	UBE4B	NP_001099032.1
	UBE4B	XP_001101836.1
Canis lupus familiaris (dog)	UBE4B	XP_005620583.1
Bos taurus (cattle)	UBE4B	NP_001095974.1
Gallus gallus (chicken)	UBE4B	XP_004947510.1
Saccharomyces cerevisiae (baker's yeast)	UFD2	NP_010091.2

Gene Ontology

The following ube4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ube4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45744	XM_018095832.1 Latest version!	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa45745	XM_018095833.1 Latest version!	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa62834	XM_031906349.1 Latest version!	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa62835	XM_018095832.2 Latest version!	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa62836	XM_018095833.2 Latest version!	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa62837	XM_004916027.4 Latest version!	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa12996	XM_004916027.3 Latest version!	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ube4b ( XM_018095832.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951321.1 Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa45744
Clone ID Related Accession (Same CDS sequence)	XM_018095832.1
Accession Version	XM_018095832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin conjugation factor E4 B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTCTC TATGGTAGTA GTCCAGCTTC CTCTTACAGT 
CTGGCTTCTG GTATTCCTTT GTGTTGTTCA CCTACCTTTC CACTTCCTAA TACTCCTCAA 
ACTACTGTGA GGGAATCTGT TCCTGGCAGC CCTCGCCTTG TGCATTCAAG CCCATCAGGA 
CCACGTTATC ACCCCTACAT GATGACACAT TCAAGACCTT TGCCCATTCC TTCCAACTTA 
AGGTCTTCGA CTTCTTTAAT GTATGTTTCA TCAAGCCCTC AAGTTCTTTC CAGCAGCCCT 
CGAGCAGTGC TCTCCAGCTC ACCTAGACAG AGACCAACCT CAGCTGCAAA TTTTGTTGCA 
TTTTCTCCCA GAATGTCCCC AAGAGAAATC CAGAATCCTA CTAGTGCTAC TAGCAGTACA 
GAATCCTGTG CTGATCACTT CACTATTGAA ACTTGCAAAG AAACAGAGAT GCTCAACTAC 
CTCATTGAAT GCTTCGATAG AGTGGGAATT GAAGAGAGGA AAGCACCAAA GATGTGCAGC 
CAGCCAGATG TGAGTCAACT CCTGAGCAAT ATTCGATTAC AGTGTATTTG CCATGCTGCT 
CTTGTGTTAC AGGGGGCCCT CACACAGCCA AGGTCTTTGC AACAGCCATC TTTGGTGGTA 
CCATACATGC TCTGCAGAAA CCTACCTTTT GGGTTTATTC AAGAGTTAGT AAGGACTACA 
TACCAAGATG AAGAAGTGTT TAAGCATATC TTTGTTCCCA TTCTTCAAGG CTTATCTCAA 
GCTGCTACAG AGTGTTCTCT GGACAGTGAT AATTTTAAAT ACCCTCTAAT GGCATTGGGT 
GAGCTTTGTG AAATTAAGTT TGGGAAGACT CATCCTGTCT GCTGTTTGAT AACCTCTTTG 
CCCTTATGGT TGCCGAAGCC ACTGAGCCCT GCAAGTGGAC GTGAACTTCA ACGACTTTCA 
TTTCTTGGTT CATATTTTAG TCTCTCCGTC TTTGCTGAAG ATGATAATAA AGTAGTCGAA 
AAATATTTTT CTGGACCAGC TATAACTTTG GAAAATACCC GCGTAGTCAG CCAATCTTTG 
CAACATTACT TGGAGTCTGC CAGACAAGAA TTGTTCAAGA TTATACACAG TATATTGCTA 
AATGGAGAAA CCAGAGAAGC TGCCCTTAAC TACATGGCTG CTCTTGTCAA TGCCAACATC 
AAAAAAGCAC AGATTCAGAC AGATGATAGG CTGGTTTCTA CAGATGGCTT CATGCTTAAT 
TTCCTTTGGG TGCTGCAGCA GCTCAGCTCT AAGATTAAAC TGGAGACTGT TGATCCTATG 
TATATTTTCC ACCCAAAGTG CCGCATCCTC TTACCCAGTG ATGAAACTCG CATCAAAGCT 
TCAACTGAGG AGGTGACTAG CTGGATGTCT GATATAAGTA ATGATCCATC TTTATTCTCA 
GAACCCAAGT TTCCAACAGA GTGCTTTTTC CTGACTCTTC ACGCCCATCA TCTAGCCATC 
CTACCTAGCT GCCGTCGCTA CATCCGCCGC TTACGGGCCA TCAGAGAGCT TAACAGGACT 
GTTGAAGAGC TTAAGAATAA TGAAAGTCAG TGGAAAGATT CCCCATTAGC TGCAAGACAC 
AGAGAAATGT TAAAGCGCTG CAAAACTCAA CTCAAGAAGC TGGTGCGCTG CAAAGCCTGT 
GCGGATACTG GTTTATTGGA TGAAAGTTTT CTCCGGAGAT GTTTAAATTT TTATGGAATG 
GTGATCCAGT TCCTACTACG TATCATGGAT CCTTCATACC CAAACATAAC AATGCCTTTA 
AATCTGGATA TACCCAAAAT GTTTGCAACG CTGCCTGAGT TTTATGTAGA AGATGTTGCT 
GAATTTTTGT TTTTTATTGT GCAATATGCT CCTCAGGTTC TTTTTGAACC CTGCACGCAA 
GATGTTGCAA CTTTCTTAGT CGTGCTGCTG TGCAATCAGA ACTATATACG AAATCCTTAC 
CTTGTGGCCA AGCTAGTAGA AGTCATGTTT GTGACCAATC CTGCCATCCA GCACAGAACT 
CAAAAGTTCT TTGAAATGAT TGAAAGTCAC CCTTTGTCAA CTAAACTTTT AGTCCCCTCA 
CTGATGAAAT TTTACACAGA TGTGGAGCAT ACAGGTTCCA CAAGTGAGTT CTATGATAAG 
TTCACCATTC GTTACCACAT CAGCACTATT TTTAAAAGCC TGTGGCAGAA TATGGCTCAT 
CACAGCACTT TCATGGAGGA GTTCAACTCT GGCAAGCAGT TTGTACGTTA TATAAACATG 
CTCATAAATG ATACTACATT TCTCCTGGAT GAGAGTCTAG AATCGCTTAA ACGCATCCAT 
GAAATGCAGG AGGAGATGAG AAACAAAGAA CATTGGGAAC AGTTGCCAAG GGATCAGCAG 
CAAGCACGGC AGTCACAGCT TGCACAGGAT GAGCGTGTTT CTCGTTCATA TCTTGCTCTA 
GCAACTGAGA CTGTAGATAT GTTTCACATC CTTACTAAAC AAGTGCAGAA ACCTTTCTTA 
AGACCTGAAC TAGGGCCCAG GCTAGCAGCA ATGTTAAATT TTAACCTCCA GCAATTGTGT 
GGACCAAAAT GTCGAGACCT GAAGGTCGAG AACCCAGAGA AATATGGTTT TGAACCAAAG 
AAACTTCTGG ACCAGCTCAC AGATATCTAT TTGCATTTAG ATTGTGCTCG GTTTGCAAAA 
GCTATTGCTG ATGATCAGAG ATCATACAGC AAGGAACTTT TTGAAGAAGT TATTTCAAAA 
ATGAAGAAAG CTGGAATTAA GTCTACAATT GCCATAGAAA AATTTAAGCT GCTTGCAGAG 
AAAGTTGAAG AGATTGTGGC CAGAAATGCT CGTGCAGAGA TAGATTACAG TGATGCGCCA 
GATGAATTTA GAGATCCTCT CATGGACACA CTGATGACAG AACCAGTAAG ACTTCCATCT 
GGGACCATCA TGGATCGATC TATCATTTTA AGGCATCTTC TGAATTCTTC CACAGACCCA 
TTTAACAGAC AGACACTTAC AGAAAATATG CTTGAACCAG TGCCAGAACT GAAGGAAAAA 
ATCCAGGCTT GGATGCGTGA CAAGCAAAAC GCAGATCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951321.1
CDS	329..4210
Translation	MEELSADEIRRRRLARLAGGPSSQPTTPLTSPQRENPPGPPAGAPTPGVSHQLGLNVQGITPATSPTGATGVAHRSHSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDSAAGEKSMSQVDVDSGIENMEVDENDRREKRSLPDKEPNLGSEVSEEQTLQLVCKIFRVSWKDRDRDVIFLQSLSSQFSQNPKKVYSNYKDLISQILMEVLMMSTHSKDDNPFASLTATSQPITVAQIPNRSLMLNSASKPDTSPMFCNSGSFSASSLSSLYGSSPASSYSLASGIPLCCSPTFPLPNTPQTTVRESVPGSPRLVHSSPSGPRYHPYMMTHSRPLPIPSNLRSSTSLMYVSSSPQVLSSSPRAVLSSSPRQRPTSAANFVAFSPRMSPREIQNPTSATSSTESCADHFTIETCKETEMLNYLIECFDRVGIEERKAPKMCSQPDVSQLLSNIRLQCICHAALVLQGALTQPRSLQQPSLVVPYMLCRNLPFGFIQELVRTTYQDEEVFKHIFVPILQGLSQAATECSLDSDNFKYPLMALGELCEIKFGKTHPVCCLITSLPLWLPKPLSPASGRELQRLSFLGSYFSLSVFAEDDNKVVEKYFSGPAITLENTRVVSQSLQHYLESARQELFKIIHSILLNGETREAALNYMAALVNANIKKAQIQTDDRLVSTDGFMLNFLWVLQQLSSKIKLETVDPMYIFHPKCRILLPSDETRIKASTEEVTSWMSDISNDPSLFSEPKFPTECFFLTLHAHHLAILPSCRRYIRRLRAIRELNRTVEELKNNESQWKDSPLAARHREMLKRCKTQLKKLVRCKACADTGLLDESFLRRCLNFYGMVIQFLLRIMDPSYPNITMPLNLDIPKMFATLPEFYVEDVAEFLFFIVQYAPQVLFEPCTQDVATFLVVLLCNQNYIRNPYLVAKLVEVMFVTNPAIQHRTQKFFEMIESHPLSTKLLVPSLMKFYTDVEHTGSTSEFYDKFTIRYHISTIFKSLWQNMAHHSTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEMQEEMRNKEHWEQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLHLDCARFAKAIADDQRSYSKELFEEVISKMKKAGIKSTIAIEKFKLLAEKVEEIVARNARAEIDYSDAPDEFRDPLMDTLMTEPVRLPSGTIMDRSIILRHLLNSSTDPFNRQTLTENMLEPVPELKEKIQAWMRDKQNADH

Target ORF information:

RefSeq Version	XM_018095832.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095832.1

ORF Insert Sequence:

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTCTC TATGGTAGTA GTCCAGCTTC CTCTTACAGT 
CTGGCTTCTG GTATTCCTTT GTGTTGTTCA CCTACCTTTC CACTTCCTAA TACTCCTCAA 
ACTACTGTGA GGGAATCTGT TCCTGGCAGC CCTCGCCTTG TGCATTCAAG CCCATCAGGA 
CCACGTTATC ACCCCTACAT GATGACACAT TCAAGACCTT TGCCCATTCC TTCCAACTTA 
AGGTCTTCGA CTTCTTTAAT GTATGTTTCA TCAAGCCCTC AAGTTCTTTC CAGCAGCCCT 
CGAGCAGTGC TCTCCAGCTC ACCTAGACAG AGACCAACCT CAGCTGCAAA TTTTGTTGCA 
TTTTCTCCCA GAATGTCCCC AAGAGAAATC CAGAATCCTA CTAGTGCTAC TAGCAGTACA 
GAATCCTGTG CTGATCACTT CACTATTGAA ACTTGCAAAG AAACAGAGAT GCTCAACTAC 
CTCATTGAAT GCTTCGATAG AGTGGGAATT GAAGAGAGGA AAGCACCAAA GATGTGCAGC 
CAGCCAGATG TGAGTCAACT CCTGAGCAAT ATTCGATTAC AGTGTATTTG CCATGCTGCT 
CTTGTGTTAC AGGGGGCCCT CACACAGCCA AGGTCTTTGC AACAGCCATC TTTGGTGGTA 
CCATACATGC TCTGCAGAAA CCTACCTTTT GGGTTTATTC AAGAGTTAGT AAGGACTACA 
TACCAAGATG AAGAAGTGTT TAAGCATATC TTTGTTCCCA TTCTTCAAGG CTTATCTCAA 
GCTGCTACAG AGTGTTCTCT GGACAGTGAT AATTTTAAAT ACCCTCTAAT GGCATTGGGT 
GAGCTTTGTG AAATTAAGTT TGGGAAGACT CATCCTGTCT GCTGTTTGAT AACCTCTTTG 
CCCTTATGGT TGCCGAAGCC ACTGAGCCCT GCAAGTGGAC GTGAACTTCA ACGACTTTCA 
TTTCTTGGTT CATATTTTAG TCTCTCCGTC TTTGCTGAAG ATGATAATAA AGTAGTCGAA 
AAATATTTTT CTGGACCAGC TATAACTTTG GAAAATACCC GCGTAGTCAG CCAATCTTTG 
CAACATTACT TGGAGTCTGC CAGACAAGAA TTGTTCAAGA TTATACACAG TATATTGCTA 
AATGGAGAAA CCAGAGAAGC TGCCCTTAAC TACATGGCTG CTCTTGTCAA TGCCAACATC 
AAAAAAGCAC AGATTCAGAC AGATGATAGG CTGGTTTCTA CAGATGGCTT CATGCTTAAT 
TTCCTTTGGG TGCTGCAGCA GCTCAGCTCT AAGATTAAAC TGGAGACTGT TGATCCTATG 
TATATTTTCC ACCCAAAGTG CCGCATCCTC TTACCCAGTG ATGAAACTCG CATCAAAGCT 
TCAACTGAGG AGGTGACTAG CTGGATGTCT GATATAAGTA ATGATCCATC TTTATTCTCA 
GAACCCAAGT TTCCAACAGA GTGCTTTTTC CTGACTCTTC ACGCCCATCA TCTAGCCATC 
CTACCTAGCT GCCGTCGCTA CATCCGCCGC TTACGGGCCA TCAGAGAGCT TAACAGGACT 
GTTGAAGAGC TTAAGAATAA TGAAAGTCAG TGGAAAGATT CCCCATTAGC TGCAAGACAC 
AGAGAAATGT TAAAGCGCTG CAAAACTCAA CTCAAGAAGC TGGTGCGCTG CAAAGCCTGT 
GCGGATACTG GTTTATTGGA TGAAAGTTTT CTCCGGAGAT GTTTAAATTT TTATGGAATG 
GTGATCCAGT TCCTACTACG TATCATGGAT CCTTCATACC CAAACATAAC AATGCCTTTA 
AATCTGGATA TACCCAAAAT GTTTGCAACG CTGCCTGAGT TTTATGTAGA AGATGTTGCT 
GAATTTTTGT TTTTTATTGT GCAATATGCT CCTCAGGTTC TTTTTGAACC CTGCACGCAA 
GATGTTGCAA CTTTCTTAGT CGTGCTGCTG TGCAATCAGA ACTATATACG AAATCCTTAC 
CTTGTGGCCA AGCTAGTAGA AGTCATGTTT GTGACCAATC CTGCCATCCA GCACAGAACT 
CAAAAGTTCT TTGAAATGAT TGAAAGTCAC CCTTTGTCAA CTAAACTTTT AGTCCCCTCA 
CTGATGAAAT TTTACACAGA TGTGGAGCAT ACAGGTTCCA CAAGTGAGTT CTATGATAAG 
TTCACCATTC GTTACCACAT CAGCACTATT TTTAAAAGCC TGTGGCAGAA TATGGCTCAT 
CACAGCACTT TCATGGAGGA GTTCAACTCT GGCAAGCAGT TTGTACGTTA TATAAACATG 
CTCATAAATG ATACTACATT TCTCCTGGAT GAGAGTCTAG AATCGCTTAA ACGCATCCAT 
GAAATGCAGG AGGAGATGAG AAACAAAGAA CATTGGGAAC AGTTGCCAAG GGATCAGCAG 
CAAGCACGGC AGTCACAGCT TGCACAGGAT GAGCGTGTTT CTCGTTCATA TCTTGCTCTA 
GCAACTGAGA CTGTAGATAT GTTTCACATC CTTACTAAAC AAGTGCAGAA ACCTTTCTTA 
AGACCTGAAC TAGGGCCCAG GCTAGCAGCA ATGTTAAATT TTAACCTCCA GCAATTGTGT 
GGACCAAAAT GTCGAGACCT GAAGGTCGAG AACCCAGAGA AATATGGTTT TGAACCAAAG 
AAACTTCTGG ACCAGCTCAC AGATATCTAT TTGCATTTAG ATTGTGCTCG GTTTGCAAAA 
GCTATTGCTG ATGATCAGAG ATCATACAGC AAGGAACTTT TTGAAGAAGT TATTTCAAAA 
ATGAAGAAAG CTGGAATTAA GTCTACAATT GCCATAGAAA AATTTAAGCT GCTTGCAGAG 
AAAGTTGAAG AGATTGTGGC CAGAAATGCT CGTGCAGAGA TAGATTACAG TGATGCGCCA 
GATGAATTTA GAGATCCTCT CATGGACACA CTGATGACAG AACCAGTAAG ACTTCCATCT 
GGGACCATCA TGGATCGATC TATCATTTTA AGGCATCTTC TGAATTCTTC CACAGACCCA 
TTTAACAGAC AGACACTTAC AGAAAATATG CTTGAACCAG TGCCAGAACT GAAGGAAAAA 
ATCCAGGCTT GGATGCGTGA CAAGCAAAAC GCAGATCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4b ( XM_018095833.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951322.1 Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa45745
Clone ID Related Accession (Same CDS sequence)	XM_018095833.1
Accession Version	XM_018095833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin conjugation factor E4 B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTACA TACGACAGCC ATATGTCTCT TCTTTCCCTC 
ACTTGTCCCG ATGAGGCTGA GGGCAGCAGT GATGAAGAGA ATCATGAAGA GGAATTTTCT 
TGTGTCCAGT TTGGTTCCAG TGCTACTAGC AGTACAGAAT CCTGTGCTGA TCACTTCACT 
ATTGAAACTT GCAAAGAAAC AGAGATGCTC AACTACCTCA TTGAATGCTT CGATAGAGTG 
GGAATTGAAG AGAGGAAAGC ACCAAAGATG TGCAGCCAGC CAGATGTGAG TCAACTCCTG 
AGCAATATTC GATTACAGTG TATTTGCCAT GCTGCTCTTG TGTTACAGGG GGCCCTCACA 
CAGCCAAGGT CTTTGCAACA GCCATCTTTG GTGGTACCAT ACATGCTCTG CAGAAACCTA 
CCTTTTGGGT TTATTCAAGA GTTAGTAAGG ACTACATACC AAGATGAAGA AGTGTTTAAG 
CATATCTTTG TTCCCATTCT TCAAGGCTTA TCTCAAGCTG CTACAGAGTG TTCTCTGGAC 
AGTGATAATT TTAAATACCC TCTAATGGCA TTGGGTGAGC TTTGTGAAAT TAAGTTTGGG 
AAGACTCATC CTGTCTGCTG TTTGATAACC TCTTTGCCCT TATGGTTGCC GAAGCCACTG 
AGCCCTGCAA GTGGACGTGA ACTTCAACGA CTTTCATTTC TTGGTTCATA TTTTAGTCTC 
TCCGTCTTTG CTGAAGATGA TAATAAAGTA GTCGAAAAAT ATTTTTCTGG ACCAGCTATA 
ACTTTGGAAA ATACCCGCGT AGTCAGCCAA TCTTTGCAAC ATTACTTGGA GTCTGCCAGA 
CAAGAATTGT TCAAGATTAT ACACAGTATA TTGCTAAATG GAGAAACCAG AGAAGCTGCC 
CTTAACTACA TGGCTGCTCT TGTCAATGCC AACATCAAAA AAGCACAGAT TCAGACAGAT 
GATAGGCTGG TTTCTACAGA TGGCTTCATG CTTAATTTCC TTTGGGTGCT GCAGCAGCTC 
AGCTCTAAGA TTAAACTGGA GACTGTTGAT CCTATGTATA TTTTCCACCC AAAGTGCCGC 
ATCCTCTTAC CCAGTGATGA AACTCGCATC AAAGCTTCAA CTGAGGAGGT GACTAGCTGG 
ATGTCTGATA TAAGTAATGA TCCATCTTTA TTCTCAGAAC CCAAGTTTCC AACAGAGTGC 
TTTTTCCTGA CTCTTCACGC CCATCATCTA GCCATCCTAC CTAGCTGCCG TCGCTACATC 
CGCCGCTTAC GGGCCATCAG AGAGCTTAAC AGGACTGTTG AAGAGCTTAA GAATAATGAA 
AGTCAGTGGA AAGATTCCCC ATTAGCTGCA AGACACAGAG AAATGTTAAA GCGCTGCAAA 
ACTCAACTCA AGAAGCTGGT GCGCTGCAAA GCCTGTGCGG ATACTGGTTT ATTGGATGAA 
AGTTTTCTCC GGAGATGTTT AAATTTTTAT GGAATGGTGA TCCAGTTCCT ACTACGTATC 
ATGGATCCTT CATACCCAAA CATAACAATG CCTTTAAATC TGGATATACC CAAAATGTTT 
GCAACGCTGC CTGAGTTTTA TGTAGAAGAT GTTGCTGAAT TTTTGTTTTT TATTGTGCAA 
TATGCTCCTC AGGTTCTTTT TGAACCCTGC ACGCAAGATG TTGCAACTTT CTTAGTCGTG 
CTGCTGTGCA ATCAGAACTA TATACGAAAT CCTTACCTTG TGGCCAAGCT AGTAGAAGTC 
ATGTTTGTGA CCAATCCTGC CATCCAGCAC AGAACTCAAA AGTTCTTTGA AATGATTGAA 
AGTCACCCTT TGTCAACTAA ACTTTTAGTC CCCTCACTGA TGAAATTTTA CACAGATGTG 
GAGCATACAG GTTCCACAAG TGAGTTCTAT GATAAGTTCA CCATTCGTTA CCACATCAGC 
ACTATTTTTA AAAGCCTGTG GCAGAATATG GCTCATCACA GCACTTTCAT GGAGGAGTTC 
AACTCTGGCA AGCAGTTTGT ACGTTATATA AACATGCTCA TAAATGATAC TACATTTCTC 
CTGGATGAGA GTCTAGAATC GCTTAAACGC ATCCATGAAA TGCAGGAGGA GATGAGAAAC 
AAAGAACATT GGGAACAGTT GCCAAGGGAT CAGCAGCAAG CACGGCAGTC ACAGCTTGCA 
CAGGATGAGC GTGTTTCTCG TTCATATCTT GCTCTAGCAA CTGAGACTGT AGATATGTTT 
CACATCCTTA CTAAACAAGT GCAGAAACCT TTCTTAAGAC CTGAACTAGG GCCCAGGCTA 
GCAGCAATGT TAAATTTTAA CCTCCAGCAA TTGTGTGGAC CAAAATGTCG AGACCTGAAG 
GTCGAGAACC CAGAGAAATA TGGTTTTGAA CCAAAGAAAC TTCTGGACCA GCTCACAGAT 
ATCTATTTGC ATTTAGATTG TGCTCGGTTT GCAAAAGCTA TTGCTGATGA TCAGAGATCA 
TACAGCAAGG AACTTTTTGA AGAAGTTATT TCAAAAATGA AGAAAGCTGG AATTAAGTCT 
ACAATTGCCA TAGAAAAATT TAAGCTGCTT GCAGAGAAAG TTGAAGAGAT TGTGGCCAGA 
AATGCTCGTG CAGAGATAGA TTACAGTGAT GCGCCAGATG AATTTAGAGA TCCTCTCATG 
GACACACTGA TGACAGAACC AGTAAGACTT CCATCTGGGA CCATCATGGA TCGATCTATC 
ATTTTAAGGC ATCTTCTGAA TTCTTCCACA GACCCATTTA ACAGACAGAC ACTTACAGAA 
AATATGCTTG AACCAGTGCC AGAACTGAAG GAAAAAATCC AGGCTTGGAT GCGTGACAAG 
CAAAACGCAG ATCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951322.1
CDS	327..3944
Translation	MEELSADEIRRRRLARLAGGPSSQPTTPLTSPQRENPPGPPAGAPTPGVSHQLGLNVQGITPATSPTGATGVAHRSHSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDSAAGEKSMSQVDVDSGIENMEVDENDRREKRSLPDKEPNLGSEVSEEQTLQLVCKIFRVSWKDRDRDVIFLQSLSSQFSQNPKKVYSNYKDLISQILMEVLMMSTHSKDDNPFASLTATSQPITVAQIPNRSLMLNSASKPDTSPMFCNSGSFSASSLSSTYDSHMSLLSLTCPDEAEGSSDEENHEEEFSCVQFGSSATSSTESCADHFTIETCKETEMLNYLIECFDRVGIEERKAPKMCSQPDVSQLLSNIRLQCICHAALVLQGALTQPRSLQQPSLVVPYMLCRNLPFGFIQELVRTTYQDEEVFKHIFVPILQGLSQAATECSLDSDNFKYPLMALGELCEIKFGKTHPVCCLITSLPLWLPKPLSPASGRELQRLSFLGSYFSLSVFAEDDNKVVEKYFSGPAITLENTRVVSQSLQHYLESARQELFKIIHSILLNGETREAALNYMAALVNANIKKAQIQTDDRLVSTDGFMLNFLWVLQQLSSKIKLETVDPMYIFHPKCRILLPSDETRIKASTEEVTSWMSDISNDPSLFSEPKFPTECFFLTLHAHHLAILPSCRRYIRRLRAIRELNRTVEELKNNESQWKDSPLAARHREMLKRCKTQLKKLVRCKACADTGLLDESFLRRCLNFYGMVIQFLLRIMDPSYPNITMPLNLDIPKMFATLPEFYVEDVAEFLFFIVQYAPQVLFEPCTQDVATFLVVLLCNQNYIRNPYLVAKLVEVMFVTNPAIQHRTQKFFEMIESHPLSTKLLVPSLMKFYTDVEHTGSTSEFYDKFTIRYHISTIFKSLWQNMAHHSTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEMQEEMRNKEHWEQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLHLDCARFAKAIADDQRSYSKELFEEVISKMKKAGIKSTIAIEKFKLLAEKVEEIVARNARAEIDYSDAPDEFRDPLMDTLMTEPVRLPSGTIMDRSIILRHLLNSSTDPFNRQTLTENMLEPVPELKEKIQAWMRDKQNADH

Target ORF information:

RefSeq Version	XM_018095833.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095833.1

ORF Insert Sequence:

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTACA TACGACAGCC ATATGTCTCT TCTTTCCCTC 
ACTTGTCCCG ATGAGGCTGA GGGCAGCAGT GATGAAGAGA ATCATGAAGA GGAATTTTCT 
TGTGTCCAGT TTGGTTCCAG TGCTACTAGC AGTACAGAAT CCTGTGCTGA TCACTTCACT 
ATTGAAACTT GCAAAGAAAC AGAGATGCTC AACTACCTCA TTGAATGCTT CGATAGAGTG 
GGAATTGAAG AGAGGAAAGC ACCAAAGATG TGCAGCCAGC CAGATGTGAG TCAACTCCTG 
AGCAATATTC GATTACAGTG TATTTGCCAT GCTGCTCTTG TGTTACAGGG GGCCCTCACA 
CAGCCAAGGT CTTTGCAACA GCCATCTTTG GTGGTACCAT ACATGCTCTG CAGAAACCTA 
CCTTTTGGGT TTATTCAAGA GTTAGTAAGG ACTACATACC AAGATGAAGA AGTGTTTAAG 
CATATCTTTG TTCCCATTCT TCAAGGCTTA TCTCAAGCTG CTACAGAGTG TTCTCTGGAC 
AGTGATAATT TTAAATACCC TCTAATGGCA TTGGGTGAGC TTTGTGAAAT TAAGTTTGGG 
AAGACTCATC CTGTCTGCTG TTTGATAACC TCTTTGCCCT TATGGTTGCC GAAGCCACTG 
AGCCCTGCAA GTGGACGTGA ACTTCAACGA CTTTCATTTC TTGGTTCATA TTTTAGTCTC 
TCCGTCTTTG CTGAAGATGA TAATAAAGTA GTCGAAAAAT ATTTTTCTGG ACCAGCTATA 
ACTTTGGAAA ATACCCGCGT AGTCAGCCAA TCTTTGCAAC ATTACTTGGA GTCTGCCAGA 
CAAGAATTGT TCAAGATTAT ACACAGTATA TTGCTAAATG GAGAAACCAG AGAAGCTGCC 
CTTAACTACA TGGCTGCTCT TGTCAATGCC AACATCAAAA AAGCACAGAT TCAGACAGAT 
GATAGGCTGG TTTCTACAGA TGGCTTCATG CTTAATTTCC TTTGGGTGCT GCAGCAGCTC 
AGCTCTAAGA TTAAACTGGA GACTGTTGAT CCTATGTATA TTTTCCACCC AAAGTGCCGC 
ATCCTCTTAC CCAGTGATGA AACTCGCATC AAAGCTTCAA CTGAGGAGGT GACTAGCTGG 
ATGTCTGATA TAAGTAATGA TCCATCTTTA TTCTCAGAAC CCAAGTTTCC AACAGAGTGC 
TTTTTCCTGA CTCTTCACGC CCATCATCTA GCCATCCTAC CTAGCTGCCG TCGCTACATC 
CGCCGCTTAC GGGCCATCAG AGAGCTTAAC AGGACTGTTG AAGAGCTTAA GAATAATGAA 
AGTCAGTGGA AAGATTCCCC ATTAGCTGCA AGACACAGAG AAATGTTAAA GCGCTGCAAA 
ACTCAACTCA AGAAGCTGGT GCGCTGCAAA GCCTGTGCGG ATACTGGTTT ATTGGATGAA 
AGTTTTCTCC GGAGATGTTT AAATTTTTAT GGAATGGTGA TCCAGTTCCT ACTACGTATC 
ATGGATCCTT CATACCCAAA CATAACAATG CCTTTAAATC TGGATATACC CAAAATGTTT 
GCAACGCTGC CTGAGTTTTA TGTAGAAGAT GTTGCTGAAT TTTTGTTTTT TATTGTGCAA 
TATGCTCCTC AGGTTCTTTT TGAACCCTGC ACGCAAGATG TTGCAACTTT CTTAGTCGTG 
CTGCTGTGCA ATCAGAACTA TATACGAAAT CCTTACCTTG TGGCCAAGCT AGTAGAAGTC 
ATGTTTGTGA CCAATCCTGC CATCCAGCAC AGAACTCAAA AGTTCTTTGA AATGATTGAA 
AGTCACCCTT TGTCAACTAA ACTTTTAGTC CCCTCACTGA TGAAATTTTA CACAGATGTG 
GAGCATACAG GTTCCACAAG TGAGTTCTAT GATAAGTTCA CCATTCGTTA CCACATCAGC 
ACTATTTTTA AAAGCCTGTG GCAGAATATG GCTCATCACA GCACTTTCAT GGAGGAGTTC 
AACTCTGGCA AGCAGTTTGT ACGTTATATA AACATGCTCA TAAATGATAC TACATTTCTC 
CTGGATGAGA GTCTAGAATC GCTTAAACGC ATCCATGAAA TGCAGGAGGA GATGAGAAAC 
AAAGAACATT GGGAACAGTT GCCAAGGGAT CAGCAGCAAG CACGGCAGTC ACAGCTTGCA 
CAGGATGAGC GTGTTTCTCG TTCATATCTT GCTCTAGCAA CTGAGACTGT AGATATGTTT 
CACATCCTTA CTAAACAAGT GCAGAAACCT TTCTTAAGAC CTGAACTAGG GCCCAGGCTA 
GCAGCAATGT TAAATTTTAA CCTCCAGCAA TTGTGTGGAC CAAAATGTCG AGACCTGAAG 
GTCGAGAACC CAGAGAAATA TGGTTTTGAA CCAAAGAAAC TTCTGGACCA GCTCACAGAT 
ATCTATTTGC ATTTAGATTG TGCTCGGTTT GCAAAAGCTA TTGCTGATGA TCAGAGATCA 
TACAGCAAGG AACTTTTTGA AGAAGTTATT TCAAAAATGA AGAAAGCTGG AATTAAGTCT 
ACAATTGCCA TAGAAAAATT TAAGCTGCTT GCAGAGAAAG TTGAAGAGAT TGTGGCCAGA 
AATGCTCGTG CAGAGATAGA TTACAGTGAT GCGCCAGATG AATTTAGAGA TCCTCTCATG 
GACACACTGA TGACAGAACC AGTAAGACTT CCATCTGGGA CCATCATGGA TCGATCTATC 
ATTTTAAGGC ATCTTCTGAA TTCTTCCACA GACCCATTTA ACAGACAGAC ACTTACAGAA 
AATATGCTTG AACCAGTGCC AGAACTGAAG GAAAAAATCC AGGCTTGGAT GCGTGACAAG 
CAAAACGCAG ATCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4b ( XM_031906349.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031762209.1 Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X1, mRNA.

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CloneID	OXa62834
Clone ID Related Accession (Same CDS sequence)	XM_031906349.1
Accession Version	XM_031906349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin conjugation factor E4 B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTCTC TATGGTAGTA GTCCAGCTTC CTCTTACAGT 
CTGGCTTCTG GTATTCCTTT GTGTTGTTCA CCTACCTTTC CACTTCCTAA TACTCCTCAA 
ACTACTGTGA GGGAATCTGT TCCTGGCAGC CCTCGCCTTG TGCATTCAAG CCCATCAGGA 
CCACGTTATC ACCCCTACAT GATGACACAT TCAAGACCTT TGCCCATTCC TTCCAACTTA 
AGGTCTTCGA CTTCTTTAAT GTATGTTTCA TCAAGCCCTC AAGTTCTTTC CAGCAGCCCT 
CGAGCAGTGC TCTCCAGCTC ACCTAGACAG AGACCAACCT CAGCTGCAAA TTTTGTTGCA 
TTTTCTCCCA GAATGTCCCC AAGAGAAATC CAGAATCCTA CTAGTACATA CGACAGCCAT 
ATGTCTCTTC TTTCCCTCAC TTGTCCCGAT GAGGCTGAGG GCAGCAGTGA TGAAGAGAAT 
CATGAAGAGG AATTTTCTTG TGTCCAGTTT GGTTCCAGTG CTACTAGCAG TACAGAATCC 
TGTGCTGATC ACTTCACTAT TGAAACTTGC AAAGAAACAG AGATGCTCAA CTACCTCATT 
GAATGCTTCG ATAGAGTGGG AATTGAAGAG AGGAAAGCAC CAAAGATGTG CAGCCAGCCA 
GATGTGAGTC AACTCCTGAG CAATATTCGA TTACAGTGTA TTTGCCATGC TGCTCTTGTG 
TTACAGGGGG CCCTCACACA GCCAAGGTCT TTGCAACAGC CATCTTTGGT GGTACCATAC 
ATGCTCTGCA GAAACCTACC TTTTGGGTTT ATTCAAGAGT TAGTAAGGAC TACATACCAA 
GATGAAGAAG TGTTTAAGCA TATCTTTGTT CCCATTCTTC AAGGCTTATC TCAAGCTGCT 
ACAGAGTGTT CTCTGGACAG TGATAATTTT AAATACCCTC TAATGGCATT GGGTGAGCTT 
TGTGAAATTA AGTTTGGGAA GACTCATCCT GTCTGCTGTT TGATAACCTC TTTGCCCTTA 
TGGTTGCCGA AGCCACTGAG CCCTGCAAGT GGACGTGAAC TTCAACGACT TTCATTTCTT 
GGTTCATATT TTAGTCTCTC CGTCTTTGCT GAAGATGATA ATAAAGTAGT CGAAAAATAT 
TTTTCTGGAC CAGCTATAAC TTTGGAAAAT ACCCGCGTAG TCAGCCAATC TTTGCAACAT 
TACTTGGAGT CTGCCAGACA AGAATTGTTC AAGATTATAC ACAGTATATT GCTAAATGGA 
GAAACCAGAG AAGCTGCCCT TAACTACATG GCTGCTCTTG TCAATGCCAA CATCAAAAAA 
GCACAGATTC AGACAGATGA TAGGCTGGTT TCTACAGATG GCTTCATGCT TAATTTCCTT 
TGGGTGCTGC AGCAGCTCAG CTCTAAGATT AAACTGGAGA CTGTTGATCC TATGTATATT 
TTCCACCCAA AGTGCCGCAT CCTCTTACCC AGTGATGAAA CTCGCATCAA AGCTTCAACT 
GAGGAGGTGA CTAGCTGGAT GTCTGATATA AGTAATGATC CATCTTTATT CTCAGAACCC 
AAGTTTCCAA CAGAGTGCTT TTTCCTGACT CTACACGCCC ATCATCTAGC CATCCTACCT 
AGCTGCCGTC GCTACATCCG CCGCTTACGG GCCATCAGAG AGCTTAACAG GACTGTTGAA 
GAGCTTAAGA ATAATGAAAG TCAGTGGAAA GATTCCCCAT TAGCTGCAAG ACACAGAGAA 
ATGTTAAAGC GCTGCAAAAC TCAACTCAAG AAGCTGGTGC GCTGCAAAGC CTGTGCGGAT 
ACTGGTTTAT TGGATGAAAG TTTTCTCCGG AGATGTTTAA ATTTTTATGG AATGGTGATC 
CAGTTCCTAC TACGTATCAT GGATCCTTCA TACCCAAACA TAACAATGCC TTTAAATCTG 
GATATACCCA AAATGTTTGC AACGCTGCCT GAGTTTTATG TAGAAGATGT TGCTGAATTT 
TTGTTTTTTA TTGTGCAATA TGCTCCTCAG GTTCTTTTTG AACCCTGCAC GCAAGATGTT 
GCAACTTTCT TAGTCGTGCT GCTGTGCAAT CAGAACTATA TACGAAATCC TTACCTTGTG 
GCCAAGCTAG TAGAAGTCAT GTTTGTGACC AATCCTGCCA TCCAGCACAG AACTCAAAAG 
TTCTTTGAAA TGATTGAAAG TCACCCTTTG TCAACTAAAC TTTTAGTCCC CTCACTGATG 
AAATTTTACA CAGATGTGGA GCATACAGGT TCCACAAGTG AGTTCTATGA TAAGTTCACC 
ATTCGTTACC ACATCAGCAC TATTTTTAAA AGCCTGTGGC AGAATATGGC TCATCACAGC 
ACTTTCATGG AGGAGTTCAA CTCTGGCAAG CAGTTTGTAC GTTATATAAA CATGCTCATA 
AATGATACTA CATTTCTCCT GGATGAGAGT CTAGAATCGC TTAAACGCAT CCATGAAATG 
CAGGAGGAGA TGAGAAACAA AGAACATTGG GAACAGTTGC CAAGGGATCA GCAGCAAGCA 
CGGCAGTCAC AGCTTGCACA GGATGAGCGT GTTTCTCGTT CATATCTTGC TCTAGCAACT 
GAGACTGTAG ATATGTTTCA CATCCTTACT AAACAAGTGC AGAAACCTTT CTTAAGACCT 
GAACTAGGGC CCAGGCTAGC AGCAATGTTA AATTTTAACC TCCAGCAATT GTGTGGACCA 
AAATGTCGAG ACCTGAAGGT CGAGAACCCA GAGAAATATG GTTTTGAACC AAAGAAACTT 
CTGGACCAGC TCACAGATAT CTATTTGCAT TTAGATTGTG CTCGGTTTGC AAAAGCTATT 
GCTGATGATC AGAGATCATA CAGCAAGGAA CTTTTTGAAG AAGTTATTTC AAAAATGAAG 
AAAGCTGGAA TTAAGTCTAC AATTGCCATA GAAAAATTTA AGCTGCTTGC AGAGAAAGTT 
GAAGAGATTG TGGCCAGAAA TGCTCGTGCA GAGATAGATT ACAGTGATGC GCCAGATGAA 
TTTAGAGATC CTCTCATGGA CACACTGATG ACAGAACCAG TAAGACTTCC ATCTGGGACC 
ATCATGGATC GATCTATCAT TTTAAGGCAT CTTCTGAATT CTTCCACAGA CCCATTTAAC 
AGACAGACAC TTACAGAAAA TATGCTTGAA CCAGTGCCAG AACTGAAGGA AAAAATCCAG 
GCTTGGATGC GTGACAAGCA AAACGCAGAT CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031762209.1
CDS	322..4317
Translation	MEELSADEIRRRRLARLAGGPSSQPTTPLTSPQRENPPGPPAGAPTPGVSHQLGLNVQGITPATSPTGATGVAHRSHSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDSAAGEKSMSQVDVDSGIENMEVDENDRREKRSLPDKEPNLGSEVSEEQTLQLVCKIFRVSWKDRDRDVIFLQSLSSQFSQNPKKVYSNYKDLISQILMEVLMMSTHSKDDNPFASLTATSQPITVAQIPNRSLMLNSASKPDTSPMFCNSGSFSASSLSSLYGSSPASSYSLASGIPLCCSPTFPLPNTPQTTVRESVPGSPRLVHSSPSGPRYHPYMMTHSRPLPIPSNLRSSTSLMYVSSSPQVLSSSPRAVLSSSPRQRPTSAANFVAFSPRMSPREIQNPTSTYDSHMSLLSLTCPDEAEGSSDEENHEEEFSCVQFGSSATSSTESCADHFTIETCKETEMLNYLIECFDRVGIEERKAPKMCSQPDVSQLLSNIRLQCICHAALVLQGALTQPRSLQQPSLVVPYMLCRNLPFGFIQELVRTTYQDEEVFKHIFVPILQGLSQAATECSLDSDNFKYPLMALGELCEIKFGKTHPVCCLITSLPLWLPKPLSPASGRELQRLSFLGSYFSLSVFAEDDNKVVEKYFSGPAITLENTRVVSQSLQHYLESARQELFKIIHSILLNGETREAALNYMAALVNANIKKAQIQTDDRLVSTDGFMLNFLWVLQQLSSKIKLETVDPMYIFHPKCRILLPSDETRIKASTEEVTSWMSDISNDPSLFSEPKFPTECFFLTLHAHHLAILPSCRRYIRRLRAIRELNRTVEELKNNESQWKDSPLAARHREMLKRCKTQLKKLVRCKACADTGLLDESFLRRCLNFYGMVIQFLLRIMDPSYPNITMPLNLDIPKMFATLPEFYVEDVAEFLFFIVQYAPQVLFEPCTQDVATFLVVLLCNQNYIRNPYLVAKLVEVMFVTNPAIQHRTQKFFEMIESHPLSTKLLVPSLMKFYTDVEHTGSTSEFYDKFTIRYHISTIFKSLWQNMAHHSTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEMQEEMRNKEHWEQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLHLDCARFAKAIADDQRSYSKELFEEVISKMKKAGIKSTIAIEKFKLLAEKVEEIVARNARAEIDYSDAPDEFRDPLMDTLMTEPVRLPSGTIMDRSIILRHLLNSSTDPFNRQTLTENMLEPVPELKEKIQAWMRDKQNADH

Target ORF information:

RefSeq Version	XM_031906349.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031906349.1

ORF Insert Sequence:

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTCTC TATGGTAGTA GTCCAGCTTC CTCTTACAGT 
CTGGCTTCTG GTATTCCTTT GTGTTGTTCA CCTACCTTTC CACTTCCTAA TACTCCTCAA 
ACTACTGTGA GGGAATCTGT TCCTGGCAGC CCTCGCCTTG TGCATTCAAG CCCATCAGGA 
CCACGTTATC ACCCCTACAT GATGACACAT TCAAGACCTT TGCCCATTCC TTCCAACTTA 
AGGTCTTCGA CTTCTTTAAT GTATGTTTCA TCAAGCCCTC AAGTTCTTTC CAGCAGCCCT 
CGAGCAGTGC TCTCCAGCTC ACCTAGACAG AGACCAACCT CAGCTGCAAA TTTTGTTGCA 
TTTTCTCCCA GAATGTCCCC AAGAGAAATC CAGAATCCTA CTAGTACATA CGACAGCCAT 
ATGTCTCTTC TTTCCCTCAC TTGTCCCGAT GAGGCTGAGG GCAGCAGTGA TGAAGAGAAT 
CATGAAGAGG AATTTTCTTG TGTCCAGTTT GGTTCCAGTG CTACTAGCAG TACAGAATCC 
TGTGCTGATC ACTTCACTAT TGAAACTTGC AAAGAAACAG AGATGCTCAA CTACCTCATT 
GAATGCTTCG ATAGAGTGGG AATTGAAGAG AGGAAAGCAC CAAAGATGTG CAGCCAGCCA 
GATGTGAGTC AACTCCTGAG CAATATTCGA TTACAGTGTA TTTGCCATGC TGCTCTTGTG 
TTACAGGGGG CCCTCACACA GCCAAGGTCT TTGCAACAGC CATCTTTGGT GGTACCATAC 
ATGCTCTGCA GAAACCTACC TTTTGGGTTT ATTCAAGAGT TAGTAAGGAC TACATACCAA 
GATGAAGAAG TGTTTAAGCA TATCTTTGTT CCCATTCTTC AAGGCTTATC TCAAGCTGCT 
ACAGAGTGTT CTCTGGACAG TGATAATTTT AAATACCCTC TAATGGCATT GGGTGAGCTT 
TGTGAAATTA AGTTTGGGAA GACTCATCCT GTCTGCTGTT TGATAACCTC TTTGCCCTTA 
TGGTTGCCGA AGCCACTGAG CCCTGCAAGT GGACGTGAAC TTCAACGACT TTCATTTCTT 
GGTTCATATT TTAGTCTCTC CGTCTTTGCT GAAGATGATA ATAAAGTAGT CGAAAAATAT 
TTTTCTGGAC CAGCTATAAC TTTGGAAAAT ACCCGCGTAG TCAGCCAATC TTTGCAACAT 
TACTTGGAGT CTGCCAGACA AGAATTGTTC AAGATTATAC ACAGTATATT GCTAAATGGA 
GAAACCAGAG AAGCTGCCCT TAACTACATG GCTGCTCTTG TCAATGCCAA CATCAAAAAA 
GCACAGATTC AGACAGATGA TAGGCTGGTT TCTACAGATG GCTTCATGCT TAATTTCCTT 
TGGGTGCTGC AGCAGCTCAG CTCTAAGATT AAACTGGAGA CTGTTGATCC TATGTATATT 
TTCCACCCAA AGTGCCGCAT CCTCTTACCC AGTGATGAAA CTCGCATCAA AGCTTCAACT 
GAGGAGGTGA CTAGCTGGAT GTCTGATATA AGTAATGATC CATCTTTATT CTCAGAACCC 
AAGTTTCCAA CAGAGTGCTT TTTCCTGACT CTACACGCCC ATCATCTAGC CATCCTACCT 
AGCTGCCGTC GCTACATCCG CCGCTTACGG GCCATCAGAG AGCTTAACAG GACTGTTGAA 
GAGCTTAAGA ATAATGAAAG TCAGTGGAAA GATTCCCCAT TAGCTGCAAG ACACAGAGAA 
ATGTTAAAGC GCTGCAAAAC TCAACTCAAG AAGCTGGTGC GCTGCAAAGC CTGTGCGGAT 
ACTGGTTTAT TGGATGAAAG TTTTCTCCGG AGATGTTTAA ATTTTTATGG AATGGTGATC 
CAGTTCCTAC TACGTATCAT GGATCCTTCA TACCCAAACA TAACAATGCC TTTAAATCTG 
GATATACCCA AAATGTTTGC AACGCTGCCT GAGTTTTATG TAGAAGATGT TGCTGAATTT 
TTGTTTTTTA TTGTGCAATA TGCTCCTCAG GTTCTTTTTG AACCCTGCAC GCAAGATGTT 
GCAACTTTCT TAGTCGTGCT GCTGTGCAAT CAGAACTATA TACGAAATCC TTACCTTGTG 
GCCAAGCTAG TAGAAGTCAT GTTTGTGACC AATCCTGCCA TCCAGCACAG AACTCAAAAG 
TTCTTTGAAA TGATTGAAAG TCACCCTTTG TCAACTAAAC TTTTAGTCCC CTCACTGATG 
AAATTTTACA CAGATGTGGA GCATACAGGT TCCACAAGTG AGTTCTATGA TAAGTTCACC 
ATTCGTTACC ACATCAGCAC TATTTTTAAA AGCCTGTGGC AGAATATGGC TCATCACAGC 
ACTTTCATGG AGGAGTTCAA CTCTGGCAAG CAGTTTGTAC GTTATATAAA CATGCTCATA 
AATGATACTA CATTTCTCCT GGATGAGAGT CTAGAATCGC TTAAACGCAT CCATGAAATG 
CAGGAGGAGA TGAGAAACAA AGAACATTGG GAACAGTTGC CAAGGGATCA GCAGCAAGCA 
CGGCAGTCAC AGCTTGCACA GGATGAGCGT GTTTCTCGTT CATATCTTGC TCTAGCAACT 
GAGACTGTAG ATATGTTTCA CATCCTTACT AAACAAGTGC AGAAACCTTT CTTAAGACCT 
GAACTAGGGC CCAGGCTAGC AGCAATGTTA AATTTTAACC TCCAGCAATT GTGTGGACCA 
AAATGTCGAG ACCTGAAGGT CGAGAACCCA GAGAAATATG GTTTTGAACC AAAGAAACTT 
CTGGACCAGC TCACAGATAT CTATTTGCAT TTAGATTGTG CTCGGTTTGC AAAAGCTATT 
GCTGATGATC AGAGATCATA CAGCAAGGAA CTTTTTGAAG AAGTTATTTC AAAAATGAAG 
AAAGCTGGAA TTAAGTCTAC AATTGCCATA GAAAAATTTA AGCTGCTTGC AGAGAAAGTT 
GAAGAGATTG TGGCCAGAAA TGCTCGTGCA GAGATAGATT ACAGTGATGC GCCAGATGAA 
TTTAGAGATC CTCTCATGGA CACACTGATG ACAGAACCAG TAAGACTTCC ATCTGGGACC 
ATCATGGATC GATCTATCAT TTTAAGGCAT CTTCTGAATT CTTCCACAGA CCCATTTAAC 
AGACAGACAC TTACAGAAAA TATGCTTGAA CCAGTGCCAG AACTGAAGGA AAAAATCCAG 
GCTTGGATGC GTGACAAGCA AAACGCAGAT CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4b ( XM_018095832.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951321.1 Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa62835
Clone ID Related Accession (Same CDS sequence)	XM_018095832.2
Accession Version	XM_018095832.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin conjugation factor E4 B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTCTC TATGGTAGTA GTCCAGCTTC CTCTTACAGT 
CTGGCTTCTG GTATTCCTTT GTGTTGTTCA CCTACCTTTC CACTTCCTAA TACTCCTCAA 
ACTACTGTGA GGGAATCTGT TCCTGGCAGC CCTCGCCTTG TGCATTCAAG CCCATCAGGA 
CCACGTTATC ACCCCTACAT GATGACACAT TCAAGACCTT TGCCCATTCC TTCCAACTTA 
AGGTCTTCGA CTTCTTTAAT GTATGTTTCA TCAAGCCCTC AAGTTCTTTC CAGCAGCCCT 
CGAGCAGTGC TCTCCAGCTC ACCTAGACAG AGACCAACCT CAGCTGCAAA TTTTGTTGCA 
TTTTCTCCCA GAATGTCCCC AAGAGAAATC CAGAATCCTA CTAGTGCTAC TAGCAGTACA 
GAATCCTGTG CTGATCACTT CACTATTGAA ACTTGCAAAG AAACAGAGAT GCTCAACTAC 
CTCATTGAAT GCTTCGATAG AGTGGGAATT GAAGAGAGGA AAGCACCAAA GATGTGCAGC 
CAGCCAGATG TGAGTCAACT CCTGAGCAAT ATTCGATTAC AGTGTATTTG CCATGCTGCT 
CTTGTGTTAC AGGGGGCCCT CACACAGCCA AGGTCTTTGC AACAGCCATC TTTGGTGGTA 
CCATACATGC TCTGCAGAAA CCTACCTTTT GGGTTTATTC AAGAGTTAGT AAGGACTACA 
TACCAAGATG AAGAAGTGTT TAAGCATATC TTTGTTCCCA TTCTTCAAGG CTTATCTCAA 
GCTGCTACAG AGTGTTCTCT GGACAGTGAT AATTTTAAAT ACCCTCTAAT GGCATTGGGT 
GAGCTTTGTG AAATTAAGTT TGGGAAGACT CATCCTGTCT GCTGTTTGAT AACCTCTTTG 
CCCTTATGGT TGCCGAAGCC ACTGAGCCCT GCAAGTGGAC GTGAACTTCA ACGACTTTCA 
TTTCTTGGTT CATATTTTAG TCTCTCCGTC TTTGCTGAAG ATGATAATAA AGTAGTCGAA 
AAATATTTTT CTGGACCAGC TATAACTTTG GAAAATACCC GCGTAGTCAG CCAATCTTTG 
CAACATTACT TGGAGTCTGC CAGACAAGAA TTGTTCAAGA TTATACACAG TATATTGCTA 
AATGGAGAAA CCAGAGAAGC TGCCCTTAAC TACATGGCTG CTCTTGTCAA TGCCAACATC 
AAAAAAGCAC AGATTCAGAC AGATGATAGG CTGGTTTCTA CAGATGGCTT CATGCTTAAT 
TTCCTTTGGG TGCTGCAGCA GCTCAGCTCT AAGATTAAAC TGGAGACTGT TGATCCTATG 
TATATTTTCC ACCCAAAGTG CCGCATCCTC TTACCCAGTG ATGAAACTCG CATCAAAGCT 
TCAACTGAGG AGGTGACTAG CTGGATGTCT GATATAAGTA ATGATCCATC TTTATTCTCA 
GAACCCAAGT TTCCAACAGA GTGCTTTTTC CTGACTCTAC ACGCCCATCA TCTAGCCATC 
CTACCTAGCT GCCGTCGCTA CATCCGCCGC TTACGGGCCA TCAGAGAGCT TAACAGGACT 
GTTGAAGAGC TTAAGAATAA TGAAAGTCAG TGGAAAGATT CCCCATTAGC TGCAAGACAC 
AGAGAAATGT TAAAGCGCTG CAAAACTCAA CTCAAGAAGC TGGTGCGCTG CAAAGCCTGT 
GCGGATACTG GTTTATTGGA TGAAAGTTTT CTCCGGAGAT GTTTAAATTT TTATGGAATG 
GTGATCCAGT TCCTACTACG TATCATGGAT CCTTCATACC CAAACATAAC AATGCCTTTA 
AATCTGGATA TACCCAAAAT GTTTGCAACG CTGCCTGAGT TTTATGTAGA AGATGTTGCT 
GAATTTTTGT TTTTTATTGT GCAATATGCT CCTCAGGTTC TTTTTGAACC CTGCACGCAA 
GATGTTGCAA CTTTCTTAGT CGTGCTGCTG TGCAATCAGA ACTATATACG AAATCCTTAC 
CTTGTGGCCA AGCTAGTAGA AGTCATGTTT GTGACCAATC CTGCCATCCA GCACAGAACT 
CAAAAGTTCT TTGAAATGAT TGAAAGTCAC CCTTTGTCAA CTAAACTTTT AGTCCCCTCA 
CTGATGAAAT TTTACACAGA TGTGGAGCAT ACAGGTTCCA CAAGTGAGTT CTATGATAAG 
TTCACCATTC GTTACCACAT CAGCACTATT TTTAAAAGCC TGTGGCAGAA TATGGCTCAT 
CACAGCACTT TCATGGAGGA GTTCAACTCT GGCAAGCAGT TTGTACGTTA TATAAACATG 
CTCATAAATG ATACTACATT TCTCCTGGAT GAGAGTCTAG AATCGCTTAA ACGCATCCAT 
GAAATGCAGG AGGAGATGAG AAACAAAGAA CATTGGGAAC AGTTGCCAAG GGATCAGCAG 
CAAGCACGGC AGTCACAGCT TGCACAGGAT GAGCGTGTTT CTCGTTCATA TCTTGCTCTA 
GCAACTGAGA CTGTAGATAT GTTTCACATC CTTACTAAAC AAGTGCAGAA ACCTTTCTTA 
AGACCTGAAC TAGGGCCCAG GCTAGCAGCA ATGTTAAATT TTAACCTCCA GCAATTGTGT 
GGACCAAAAT GTCGAGACCT GAAGGTCGAG AACCCAGAGA AATATGGTTT TGAACCAAAG 
AAACTTCTGG ACCAGCTCAC AGATATCTAT TTGCATTTAG ATTGTGCTCG GTTTGCAAAA 
GCTATTGCTG ATGATCAGAG ATCATACAGC AAGGAACTTT TTGAAGAAGT TATTTCAAAA 
ATGAAGAAAG CTGGAATTAA GTCTACAATT GCCATAGAAA AATTTAAGCT GCTTGCAGAG 
AAAGTTGAAG AGATTGTGGC CAGAAATGCT CGTGCAGAGA TAGATTACAG TGATGCGCCA 
GATGAATTTA GAGATCCTCT CATGGACACA CTGATGACAG AACCAGTAAG ACTTCCATCT 
GGGACCATCA TGGATCGATC TATCATTTTA AGGCATCTTC TGAATTCTTC CACAGACCCA 
TTTAACAGAC AGACACTTAC AGAAAATATG CTTGAACCAG TGCCAGAACT GAAGGAAAAA 
ATCCAGGCTT GGATGCGTGA CAAGCAAAAC GCAGATCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951321.1
CDS	322..4203
Translation	MEELSADEIRRRRLARLAGGPSSQPTTPLTSPQRENPPGPPAGAPTPGVSHQLGLNVQGITPATSPTGATGVAHRSHSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDSAAGEKSMSQVDVDSGIENMEVDENDRREKRSLPDKEPNLGSEVSEEQTLQLVCKIFRVSWKDRDRDVIFLQSLSSQFSQNPKKVYSNYKDLISQILMEVLMMSTHSKDDNPFASLTATSQPITVAQIPNRSLMLNSASKPDTSPMFCNSGSFSASSLSSLYGSSPASSYSLASGIPLCCSPTFPLPNTPQTTVRESVPGSPRLVHSSPSGPRYHPYMMTHSRPLPIPSNLRSSTSLMYVSSSPQVLSSSPRAVLSSSPRQRPTSAANFVAFSPRMSPREIQNPTSATSSTESCADHFTIETCKETEMLNYLIECFDRVGIEERKAPKMCSQPDVSQLLSNIRLQCICHAALVLQGALTQPRSLQQPSLVVPYMLCRNLPFGFIQELVRTTYQDEEVFKHIFVPILQGLSQAATECSLDSDNFKYPLMALGELCEIKFGKTHPVCCLITSLPLWLPKPLSPASGRELQRLSFLGSYFSLSVFAEDDNKVVEKYFSGPAITLENTRVVSQSLQHYLESARQELFKIIHSILLNGETREAALNYMAALVNANIKKAQIQTDDRLVSTDGFMLNFLWVLQQLSSKIKLETVDPMYIFHPKCRILLPSDETRIKASTEEVTSWMSDISNDPSLFSEPKFPTECFFLTLHAHHLAILPSCRRYIRRLRAIRELNRTVEELKNNESQWKDSPLAARHREMLKRCKTQLKKLVRCKACADTGLLDESFLRRCLNFYGMVIQFLLRIMDPSYPNITMPLNLDIPKMFATLPEFYVEDVAEFLFFIVQYAPQVLFEPCTQDVATFLVVLLCNQNYIRNPYLVAKLVEVMFVTNPAIQHRTQKFFEMIESHPLSTKLLVPSLMKFYTDVEHTGSTSEFYDKFTIRYHISTIFKSLWQNMAHHSTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEMQEEMRNKEHWEQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLHLDCARFAKAIADDQRSYSKELFEEVISKMKKAGIKSTIAIEKFKLLAEKVEEIVARNARAEIDYSDAPDEFRDPLMDTLMTEPVRLPSGTIMDRSIILRHLLNSSTDPFNRQTLTENMLEPVPELKEKIQAWMRDKQNADH

Target ORF information:

RefSeq Version	XM_018095832.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095832.2

ORF Insert Sequence:

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTCTC TATGGTAGTA GTCCAGCTTC CTCTTACAGT 
CTGGCTTCTG GTATTCCTTT GTGTTGTTCA CCTACCTTTC CACTTCCTAA TACTCCTCAA 
ACTACTGTGA GGGAATCTGT TCCTGGCAGC CCTCGCCTTG TGCATTCAAG CCCATCAGGA 
CCACGTTATC ACCCCTACAT GATGACACAT TCAAGACCTT TGCCCATTCC TTCCAACTTA 
AGGTCTTCGA CTTCTTTAAT GTATGTTTCA TCAAGCCCTC AAGTTCTTTC CAGCAGCCCT 
CGAGCAGTGC TCTCCAGCTC ACCTAGACAG AGACCAACCT CAGCTGCAAA TTTTGTTGCA 
TTTTCTCCCA GAATGTCCCC AAGAGAAATC CAGAATCCTA CTAGTGCTAC TAGCAGTACA 
GAATCCTGTG CTGATCACTT CACTATTGAA ACTTGCAAAG AAACAGAGAT GCTCAACTAC 
CTCATTGAAT GCTTCGATAG AGTGGGAATT GAAGAGAGGA AAGCACCAAA GATGTGCAGC 
CAGCCAGATG TGAGTCAACT CCTGAGCAAT ATTCGATTAC AGTGTATTTG CCATGCTGCT 
CTTGTGTTAC AGGGGGCCCT CACACAGCCA AGGTCTTTGC AACAGCCATC TTTGGTGGTA 
CCATACATGC TCTGCAGAAA CCTACCTTTT GGGTTTATTC AAGAGTTAGT AAGGACTACA 
TACCAAGATG AAGAAGTGTT TAAGCATATC TTTGTTCCCA TTCTTCAAGG CTTATCTCAA 
GCTGCTACAG AGTGTTCTCT GGACAGTGAT AATTTTAAAT ACCCTCTAAT GGCATTGGGT 
GAGCTTTGTG AAATTAAGTT TGGGAAGACT CATCCTGTCT GCTGTTTGAT AACCTCTTTG 
CCCTTATGGT TGCCGAAGCC ACTGAGCCCT GCAAGTGGAC GTGAACTTCA ACGACTTTCA 
TTTCTTGGTT CATATTTTAG TCTCTCCGTC TTTGCTGAAG ATGATAATAA AGTAGTCGAA 
AAATATTTTT CTGGACCAGC TATAACTTTG GAAAATACCC GCGTAGTCAG CCAATCTTTG 
CAACATTACT TGGAGTCTGC CAGACAAGAA TTGTTCAAGA TTATACACAG TATATTGCTA 
AATGGAGAAA CCAGAGAAGC TGCCCTTAAC TACATGGCTG CTCTTGTCAA TGCCAACATC 
AAAAAAGCAC AGATTCAGAC AGATGATAGG CTGGTTTCTA CAGATGGCTT CATGCTTAAT 
TTCCTTTGGG TGCTGCAGCA GCTCAGCTCT AAGATTAAAC TGGAGACTGT TGATCCTATG 
TATATTTTCC ACCCAAAGTG CCGCATCCTC TTACCCAGTG ATGAAACTCG CATCAAAGCT 
TCAACTGAGG AGGTGACTAG CTGGATGTCT GATATAAGTA ATGATCCATC TTTATTCTCA 
GAACCCAAGT TTCCAACAGA GTGCTTTTTC CTGACTCTAC ACGCCCATCA TCTAGCCATC 
CTACCTAGCT GCCGTCGCTA CATCCGCCGC TTACGGGCCA TCAGAGAGCT TAACAGGACT 
GTTGAAGAGC TTAAGAATAA TGAAAGTCAG TGGAAAGATT CCCCATTAGC TGCAAGACAC 
AGAGAAATGT TAAAGCGCTG CAAAACTCAA CTCAAGAAGC TGGTGCGCTG CAAAGCCTGT 
GCGGATACTG GTTTATTGGA TGAAAGTTTT CTCCGGAGAT GTTTAAATTT TTATGGAATG 
GTGATCCAGT TCCTACTACG TATCATGGAT CCTTCATACC CAAACATAAC AATGCCTTTA 
AATCTGGATA TACCCAAAAT GTTTGCAACG CTGCCTGAGT TTTATGTAGA AGATGTTGCT 
GAATTTTTGT TTTTTATTGT GCAATATGCT CCTCAGGTTC TTTTTGAACC CTGCACGCAA 
GATGTTGCAA CTTTCTTAGT CGTGCTGCTG TGCAATCAGA ACTATATACG AAATCCTTAC 
CTTGTGGCCA AGCTAGTAGA AGTCATGTTT GTGACCAATC CTGCCATCCA GCACAGAACT 
CAAAAGTTCT TTGAAATGAT TGAAAGTCAC CCTTTGTCAA CTAAACTTTT AGTCCCCTCA 
CTGATGAAAT TTTACACAGA TGTGGAGCAT ACAGGTTCCA CAAGTGAGTT CTATGATAAG 
TTCACCATTC GTTACCACAT CAGCACTATT TTTAAAAGCC TGTGGCAGAA TATGGCTCAT 
CACAGCACTT TCATGGAGGA GTTCAACTCT GGCAAGCAGT TTGTACGTTA TATAAACATG 
CTCATAAATG ATACTACATT TCTCCTGGAT GAGAGTCTAG AATCGCTTAA ACGCATCCAT 
GAAATGCAGG AGGAGATGAG AAACAAAGAA CATTGGGAAC AGTTGCCAAG GGATCAGCAG 
CAAGCACGGC AGTCACAGCT TGCACAGGAT GAGCGTGTTT CTCGTTCATA TCTTGCTCTA 
GCAACTGAGA CTGTAGATAT GTTTCACATC CTTACTAAAC AAGTGCAGAA ACCTTTCTTA 
AGACCTGAAC TAGGGCCCAG GCTAGCAGCA ATGTTAAATT TTAACCTCCA GCAATTGTGT 
GGACCAAAAT GTCGAGACCT GAAGGTCGAG AACCCAGAGA AATATGGTTT TGAACCAAAG 
AAACTTCTGG ACCAGCTCAC AGATATCTAT TTGCATTTAG ATTGTGCTCG GTTTGCAAAA 
GCTATTGCTG ATGATCAGAG ATCATACAGC AAGGAACTTT TTGAAGAAGT TATTTCAAAA 
ATGAAGAAAG CTGGAATTAA GTCTACAATT GCCATAGAAA AATTTAAGCT GCTTGCAGAG 
AAAGTTGAAG AGATTGTGGC CAGAAATGCT CGTGCAGAGA TAGATTACAG TGATGCGCCA 
GATGAATTTA GAGATCCTCT CATGGACACA CTGATGACAG AACCAGTAAG ACTTCCATCT 
GGGACCATCA TGGATCGATC TATCATTTTA AGGCATCTTC TGAATTCTTC CACAGACCCA 
TTTAACAGAC AGACACTTAC AGAAAATATG CTTGAACCAG TGCCAGAACT GAAGGAAAAA 
ATCCAGGCTT GGATGCGTGA CAAGCAAAAC GCAGATCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4b ( XM_018095833.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951322.1 Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa62836
Clone ID Related Accession (Same CDS sequence)	XM_018095833.2
Accession Version	XM_018095833.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin conjugation factor E4 B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095833.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTACA TACGACAGCC ATATGTCTCT TCTTTCCCTC 
ACTTGTCCCG ATGAGGCTGA GGGCAGCAGT GATGAAGAGA ATCATGAAGA GGAATTTTCT 
TGTGTCCAGT TTGGTTCCAG TGCTACTAGC AGTACAGAAT CCTGTGCTGA TCACTTCACT 
ATTGAAACTT GCAAAGAAAC AGAGATGCTC AACTACCTCA TTGAATGCTT CGATAGAGTG 
GGAATTGAAG AGAGGAAAGC ACCAAAGATG TGCAGCCAGC CAGATGTGAG TCAACTCCTG 
AGCAATATTC GATTACAGTG TATTTGCCAT GCTGCTCTTG TGTTACAGGG GGCCCTCACA 
CAGCCAAGGT CTTTGCAACA GCCATCTTTG GTGGTACCAT ACATGCTCTG CAGAAACCTA 
CCTTTTGGGT TTATTCAAGA GTTAGTAAGG ACTACATACC AAGATGAAGA AGTGTTTAAG 
CATATCTTTG TTCCCATTCT TCAAGGCTTA TCTCAAGCTG CTACAGAGTG TTCTCTGGAC 
AGTGATAATT TTAAATACCC TCTAATGGCA TTGGGTGAGC TTTGTGAAAT TAAGTTTGGG 
AAGACTCATC CTGTCTGCTG TTTGATAACC TCTTTGCCCT TATGGTTGCC GAAGCCACTG 
AGCCCTGCAA GTGGACGTGA ACTTCAACGA CTTTCATTTC TTGGTTCATA TTTTAGTCTC 
TCCGTCTTTG CTGAAGATGA TAATAAAGTA GTCGAAAAAT ATTTTTCTGG ACCAGCTATA 
ACTTTGGAAA ATACCCGCGT AGTCAGCCAA TCTTTGCAAC ATTACTTGGA GTCTGCCAGA 
CAAGAATTGT TCAAGATTAT ACACAGTATA TTGCTAAATG GAGAAACCAG AGAAGCTGCC 
CTTAACTACA TGGCTGCTCT TGTCAATGCC AACATCAAAA AAGCACAGAT TCAGACAGAT 
GATAGGCTGG TTTCTACAGA TGGCTTCATG CTTAATTTCC TTTGGGTGCT GCAGCAGCTC 
AGCTCTAAGA TTAAACTGGA GACTGTTGAT CCTATGTATA TTTTCCACCC AAAGTGCCGC 
ATCCTCTTAC CCAGTGATGA AACTCGCATC AAAGCTTCAA CTGAGGAGGT GACTAGCTGG 
ATGTCTGATA TAAGTAATGA TCCATCTTTA TTCTCAGAAC CCAAGTTTCC AACAGAGTGC 
TTTTTCCTGA CTCTACACGC CCATCATCTA GCCATCCTAC CTAGCTGCCG TCGCTACATC 
CGCCGCTTAC GGGCCATCAG AGAGCTTAAC AGGACTGTTG AAGAGCTTAA GAATAATGAA 
AGTCAGTGGA AAGATTCCCC ATTAGCTGCA AGACACAGAG AAATGTTAAA GCGCTGCAAA 
ACTCAACTCA AGAAGCTGGT GCGCTGCAAA GCCTGTGCGG ATACTGGTTT ATTGGATGAA 
AGTTTTCTCC GGAGATGTTT AAATTTTTAT GGAATGGTGA TCCAGTTCCT ACTACGTATC 
ATGGATCCTT CATACCCAAA CATAACAATG CCTTTAAATC TGGATATACC CAAAATGTTT 
GCAACGCTGC CTGAGTTTTA TGTAGAAGAT GTTGCTGAAT TTTTGTTTTT TATTGTGCAA 
TATGCTCCTC AGGTTCTTTT TGAACCCTGC ACGCAAGATG TTGCAACTTT CTTAGTCGTG 
CTGCTGTGCA ATCAGAACTA TATACGAAAT CCTTACCTTG TGGCCAAGCT AGTAGAAGTC 
ATGTTTGTGA CCAATCCTGC CATCCAGCAC AGAACTCAAA AGTTCTTTGA AATGATTGAA 
AGTCACCCTT TGTCAACTAA ACTTTTAGTC CCCTCACTGA TGAAATTTTA CACAGATGTG 
GAGCATACAG GTTCCACAAG TGAGTTCTAT GATAAGTTCA CCATTCGTTA CCACATCAGC 
ACTATTTTTA AAAGCCTGTG GCAGAATATG GCTCATCACA GCACTTTCAT GGAGGAGTTC 
AACTCTGGCA AGCAGTTTGT ACGTTATATA AACATGCTCA TAAATGATAC TACATTTCTC 
CTGGATGAGA GTCTAGAATC GCTTAAACGC ATCCATGAAA TGCAGGAGGA GATGAGAAAC 
AAAGAACATT GGGAACAGTT GCCAAGGGAT CAGCAGCAAG CACGGCAGTC ACAGCTTGCA 
CAGGATGAGC GTGTTTCTCG TTCATATCTT GCTCTAGCAA CTGAGACTGT AGATATGTTT 
CACATCCTTA CTAAACAAGT GCAGAAACCT TTCTTAAGAC CTGAACTAGG GCCCAGGCTA 
GCAGCAATGT TAAATTTTAA CCTCCAGCAA TTGTGTGGAC CAAAATGTCG AGACCTGAAG 
GTCGAGAACC CAGAGAAATA TGGTTTTGAA CCAAAGAAAC TTCTGGACCA GCTCACAGAT 
ATCTATTTGC ATTTAGATTG TGCTCGGTTT GCAAAAGCTA TTGCTGATGA TCAGAGATCA 
TACAGCAAGG AACTTTTTGA AGAAGTTATT TCAAAAATGA AGAAAGCTGG AATTAAGTCT 
ACAATTGCCA TAGAAAAATT TAAGCTGCTT GCAGAGAAAG TTGAAGAGAT TGTGGCCAGA 
AATGCTCGTG CAGAGATAGA TTACAGTGAT GCGCCAGATG AATTTAGAGA TCCTCTCATG 
GACACACTGA TGACAGAACC AGTAAGACTT CCATCTGGGA CCATCATGGA TCGATCTATC 
ATTTTAAGGC ATCTTCTGAA TTCTTCCACA GACCCATTTA ACAGACAGAC ACTTACAGAA 
AATATGCTTG AACCAGTGCC AGAACTGAAG GAAAAAATCC AGGCTTGGAT GCGTGACAAG 
CAAAACGCAG ATCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951322.1
CDS	321..3938
Translation	MEELSADEIRRRRLARLAGGPSSQPTTPLTSPQRENPPGPPAGAPTPGVSHQLGLNVQGITPATSPTGATGVAHRSHSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDSAAGEKSMSQVDVDSGIENMEVDENDRREKRSLPDKEPNLGSEVSEEQTLQLVCKIFRVSWKDRDRDVIFLQSLSSQFSQNPKKVYSNYKDLISQILMEVLMMSTHSKDDNPFASLTATSQPITVAQIPNRSLMLNSASKPDTSPMFCNSGSFSASSLSSTYDSHMSLLSLTCPDEAEGSSDEENHEEEFSCVQFGSSATSSTESCADHFTIETCKETEMLNYLIECFDRVGIEERKAPKMCSQPDVSQLLSNIRLQCICHAALVLQGALTQPRSLQQPSLVVPYMLCRNLPFGFIQELVRTTYQDEEVFKHIFVPILQGLSQAATECSLDSDNFKYPLMALGELCEIKFGKTHPVCCLITSLPLWLPKPLSPASGRELQRLSFLGSYFSLSVFAEDDNKVVEKYFSGPAITLENTRVVSQSLQHYLESARQELFKIIHSILLNGETREAALNYMAALVNANIKKAQIQTDDRLVSTDGFMLNFLWVLQQLSSKIKLETVDPMYIFHPKCRILLPSDETRIKASTEEVTSWMSDISNDPSLFSEPKFPTECFFLTLHAHHLAILPSCRRYIRRLRAIRELNRTVEELKNNESQWKDSPLAARHREMLKRCKTQLKKLVRCKACADTGLLDESFLRRCLNFYGMVIQFLLRIMDPSYPNITMPLNLDIPKMFATLPEFYVEDVAEFLFFIVQYAPQVLFEPCTQDVATFLVVLLCNQNYIRNPYLVAKLVEVMFVTNPAIQHRTQKFFEMIESHPLSTKLLVPSLMKFYTDVEHTGSTSEFYDKFTIRYHISTIFKSLWQNMAHHSTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEMQEEMRNKEHWEQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLHLDCARFAKAIADDQRSYSKELFEEVISKMKKAGIKSTIAIEKFKLLAEKVEEIVARNARAEIDYSDAPDEFRDPLMDTLMTEPVRLPSGTIMDRSIILRHLLNSSTDPFNRQTLTENMLEPVPELKEKIQAWMRDKQNADH

Target ORF information:

RefSeq Version	XM_018095833.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018095833.2

ORF Insert Sequence:

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTACA TACGACAGCC ATATGTCTCT TCTTTCCCTC 
ACTTGTCCCG ATGAGGCTGA GGGCAGCAGT GATGAAGAGA ATCATGAAGA GGAATTTTCT 
TGTGTCCAGT TTGGTTCCAG TGCTACTAGC AGTACAGAAT CCTGTGCTGA TCACTTCACT 
ATTGAAACTT GCAAAGAAAC AGAGATGCTC AACTACCTCA TTGAATGCTT CGATAGAGTG 
GGAATTGAAG AGAGGAAAGC ACCAAAGATG TGCAGCCAGC CAGATGTGAG TCAACTCCTG 
AGCAATATTC GATTACAGTG TATTTGCCAT GCTGCTCTTG TGTTACAGGG GGCCCTCACA 
CAGCCAAGGT CTTTGCAACA GCCATCTTTG GTGGTACCAT ACATGCTCTG CAGAAACCTA 
CCTTTTGGGT TTATTCAAGA GTTAGTAAGG ACTACATACC AAGATGAAGA AGTGTTTAAG 
CATATCTTTG TTCCCATTCT TCAAGGCTTA TCTCAAGCTG CTACAGAGTG TTCTCTGGAC 
AGTGATAATT TTAAATACCC TCTAATGGCA TTGGGTGAGC TTTGTGAAAT TAAGTTTGGG 
AAGACTCATC CTGTCTGCTG TTTGATAACC TCTTTGCCCT TATGGTTGCC GAAGCCACTG 
AGCCCTGCAA GTGGACGTGA ACTTCAACGA CTTTCATTTC TTGGTTCATA TTTTAGTCTC 
TCCGTCTTTG CTGAAGATGA TAATAAAGTA GTCGAAAAAT ATTTTTCTGG ACCAGCTATA 
ACTTTGGAAA ATACCCGCGT AGTCAGCCAA TCTTTGCAAC ATTACTTGGA GTCTGCCAGA 
CAAGAATTGT TCAAGATTAT ACACAGTATA TTGCTAAATG GAGAAACCAG AGAAGCTGCC 
CTTAACTACA TGGCTGCTCT TGTCAATGCC AACATCAAAA AAGCACAGAT TCAGACAGAT 
GATAGGCTGG TTTCTACAGA TGGCTTCATG CTTAATTTCC TTTGGGTGCT GCAGCAGCTC 
AGCTCTAAGA TTAAACTGGA GACTGTTGAT CCTATGTATA TTTTCCACCC AAAGTGCCGC 
ATCCTCTTAC CCAGTGATGA AACTCGCATC AAAGCTTCAA CTGAGGAGGT GACTAGCTGG 
ATGTCTGATA TAAGTAATGA TCCATCTTTA TTCTCAGAAC CCAAGTTTCC AACAGAGTGC 
TTTTTCCTGA CTCTACACGC CCATCATCTA GCCATCCTAC CTAGCTGCCG TCGCTACATC 
CGCCGCTTAC GGGCCATCAG AGAGCTTAAC AGGACTGTTG AAGAGCTTAA GAATAATGAA 
AGTCAGTGGA AAGATTCCCC ATTAGCTGCA AGACACAGAG AAATGTTAAA GCGCTGCAAA 
ACTCAACTCA AGAAGCTGGT GCGCTGCAAA GCCTGTGCGG ATACTGGTTT ATTGGATGAA 
AGTTTTCTCC GGAGATGTTT AAATTTTTAT GGAATGGTGA TCCAGTTCCT ACTACGTATC 
ATGGATCCTT CATACCCAAA CATAACAATG CCTTTAAATC TGGATATACC CAAAATGTTT 
GCAACGCTGC CTGAGTTTTA TGTAGAAGAT GTTGCTGAAT TTTTGTTTTT TATTGTGCAA 
TATGCTCCTC AGGTTCTTTT TGAACCCTGC ACGCAAGATG TTGCAACTTT CTTAGTCGTG 
CTGCTGTGCA ATCAGAACTA TATACGAAAT CCTTACCTTG TGGCCAAGCT AGTAGAAGTC 
ATGTTTGTGA CCAATCCTGC CATCCAGCAC AGAACTCAAA AGTTCTTTGA AATGATTGAA 
AGTCACCCTT TGTCAACTAA ACTTTTAGTC CCCTCACTGA TGAAATTTTA CACAGATGTG 
GAGCATACAG GTTCCACAAG TGAGTTCTAT GATAAGTTCA CCATTCGTTA CCACATCAGC 
ACTATTTTTA AAAGCCTGTG GCAGAATATG GCTCATCACA GCACTTTCAT GGAGGAGTTC 
AACTCTGGCA AGCAGTTTGT ACGTTATATA AACATGCTCA TAAATGATAC TACATTTCTC 
CTGGATGAGA GTCTAGAATC GCTTAAACGC ATCCATGAAA TGCAGGAGGA GATGAGAAAC 
AAAGAACATT GGGAACAGTT GCCAAGGGAT CAGCAGCAAG CACGGCAGTC ACAGCTTGCA 
CAGGATGAGC GTGTTTCTCG TTCATATCTT GCTCTAGCAA CTGAGACTGT AGATATGTTT 
CACATCCTTA CTAAACAAGT GCAGAAACCT TTCTTAAGAC CTGAACTAGG GCCCAGGCTA 
GCAGCAATGT TAAATTTTAA CCTCCAGCAA TTGTGTGGAC CAAAATGTCG AGACCTGAAG 
GTCGAGAACC CAGAGAAATA TGGTTTTGAA CCAAAGAAAC TTCTGGACCA GCTCACAGAT 
ATCTATTTGC ATTTAGATTG TGCTCGGTTT GCAAAAGCTA TTGCTGATGA TCAGAGATCA 
TACAGCAAGG AACTTTTTGA AGAAGTTATT TCAAAAATGA AGAAAGCTGG AATTAAGTCT 
ACAATTGCCA TAGAAAAATT TAAGCTGCTT GCAGAGAAAG TTGAAGAGAT TGTGGCCAGA 
AATGCTCGTG CAGAGATAGA TTACAGTGAT GCGCCAGATG AATTTAGAGA TCCTCTCATG 
GACACACTGA TGACAGAACC AGTAAGACTT CCATCTGGGA CCATCATGGA TCGATCTATC 
ATTTTAAGGC ATCTTCTGAA TTCTTCCACA GACCCATTTA ACAGACAGAC ACTTACAGAA 
AATATGCTTG AACCAGTGCC AGAACTGAAG GAAAAAATCC AGGCTTGGAT GCGTGACAAG 
CAAAACGCAG ATCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4b ( XM_004916027.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916084.1 Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X4, mRNA.

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CloneID	OXa62837
Clone ID Related Accession (Same CDS sequence)	XM_004916027.4
Accession Version	XM_004916027.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin conjugation factor E4 B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916027.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTGCT ACTAGCAGTA CAGAATCCTG TGCTGATCAC 
TTCACTATTG AAACTTGCAA AGAAACAGAG ATGCTCAACT ACCTCATTGA ATGCTTCGAT 
AGAGTGGGAA TTGAAGAGAG GAAAGCACCA AAGATGTGCA GCCAGCCAGA TGTGAGTCAA 
CTCCTGAGCA ATATTCGATT ACAGTGTATT TGCCATGCTG CTCTTGTGTT ACAGGGGGCC 
CTCACACAGC CAAGGTCTTT GCAACAGCCA TCTTTGGTGG TACCATACAT GCTCTGCAGA 
AACCTACCTT TTGGGTTTAT TCAAGAGTTA GTAAGGACTA CATACCAAGA TGAAGAAGTG 
TTTAAGCATA TCTTTGTTCC CATTCTTCAA GGCTTATCTC AAGCTGCTAC AGAGTGTTCT 
CTGGACAGTG ATAATTTTAA ATACCCTCTA ATGGCATTGG GTGAGCTTTG TGAAATTAAG 
TTTGGGAAGA CTCATCCTGT CTGCTGTTTG ATAACCTCTT TGCCCTTATG GTTGCCGAAG 
CCACTGAGCC CTGCAAGTGG ACGTGAACTT CAACGACTTT CATTTCTTGG TTCATATTTT 
AGTCTCTCCG TCTTTGCTGA AGATGATAAT AAAGTAGTCG AAAAATATTT TTCTGGACCA 
GCTATAACTT TGGAAAATAC CCGCGTAGTC AGCCAATCTT TGCAACATTA CTTGGAGTCT 
GCCAGACAAG AATTGTTCAA GATTATACAC AGTATATTGC TAAATGGAGA AACCAGAGAA 
GCTGCCCTTA ACTACATGGC TGCTCTTGTC AATGCCAACA TCAAAAAAGC ACAGATTCAG 
ACAGATGATA GGCTGGTTTC TACAGATGGC TTCATGCTTA ATTTCCTTTG GGTGCTGCAG 
CAGCTCAGCT CTAAGATTAA ACTGGAGACT GTTGATCCTA TGTATATTTT CCACCCAAAG 
TGCCGCATCC TCTTACCCAG TGATGAAACT CGCATCAAAG CTTCAACTGA GGAGGTGACT 
AGCTGGATGT CTGATATAAG TAATGATCCA TCTTTATTCT CAGAACCCAA GTTTCCAACA 
GAGTGCTTTT TCCTGACTCT ACACGCCCAT CATCTAGCCA TCCTACCTAG CTGCCGTCGC 
TACATCCGCC GCTTACGGGC CATCAGAGAG CTTAACAGGA CTGTTGAAGA GCTTAAGAAT 
AATGAAAGTC AGTGGAAAGA TTCCCCATTA GCTGCAAGAC ACAGAGAAAT GTTAAAGCGC 
TGCAAAACTC AACTCAAGAA GCTGGTGCGC TGCAAAGCCT GTGCGGATAC TGGTTTATTG 
GATGAAAGTT TTCTCCGGAG ATGTTTAAAT TTTTATGGAA TGGTGATCCA GTTCCTACTA 
CGTATCATGG ATCCTTCATA CCCAAACATA ACAATGCCTT TAAATCTGGA TATACCCAAA 
ATGTTTGCAA CGCTGCCTGA GTTTTATGTA GAAGATGTTG CTGAATTTTT GTTTTTTATT 
GTGCAATATG CTCCTCAGGT TCTTTTTGAA CCCTGCACGC AAGATGTTGC AACTTTCTTA 
GTCGTGCTGC TGTGCAATCA GAACTATATA CGAAATCCTT ACCTTGTGGC CAAGCTAGTA 
GAAGTCATGT TTGTGACCAA TCCTGCCATC CAGCACAGAA CTCAAAAGTT CTTTGAAATG 
ATTGAAAGTC ACCCTTTGTC AACTAAACTT TTAGTCCCCT CACTGATGAA ATTTTACACA 
GATGTGGAGC ATACAGGTTC CACAAGTGAG TTCTATGATA AGTTCACCAT TCGTTACCAC 
ATCAGCACTA TTTTTAAAAG CCTGTGGCAG AATATGGCTC ATCACAGCAC TTTCATGGAG 
GAGTTCAACT CTGGCAAGCA GTTTGTACGT TATATAAACA TGCTCATAAA TGATACTACA 
TTTCTCCTGG ATGAGAGTCT AGAATCGCTT AAACGCATCC ATGAAATGCA GGAGGAGATG 
AGAAACAAAG AACATTGGGA ACAGTTGCCA AGGGATCAGC AGCAAGCACG GCAGTCACAG 
CTTGCACAGG ATGAGCGTGT TTCTCGTTCA TATCTTGCTC TAGCAACTGA GACTGTAGAT 
ATGTTTCACA TCCTTACTAA ACAAGTGCAG AAACCTTTCT TAAGACCTGA ACTAGGGCCC 
AGGCTAGCAG CAATGTTAAA TTTTAACCTC CAGCAATTGT GTGGACCAAA ATGTCGAGAC 
CTGAAGGTCG AGAACCCAGA GAAATATGGT TTTGAACCAA AGAAACTTCT GGACCAGCTC 
ACAGATATCT ATTTGCATTT AGATTGTGCT CGGTTTGCAA AAGCTATTGC TGATGATCAG 
AGATCATACA GCAAGGAACT TTTTGAAGAA GTTATTTCAA AAATGAAGAA AGCTGGAATT 
AAGTCTACAA TTGCCATAGA AAAATTTAAG CTGCTTGCAG AGAAAGTTGA AGAGATTGTG 
GCCAGAAATG CTCGTGCAGA GATAGATTAC AGTGATGCGC CAGATGAATT TAGAGATCCT 
CTCATGGACA CACTGATGAC AGAACCAGTA AGACTTCCAT CTGGGACCAT CATGGATCGA 
TCTATCATTT TAAGGCATCT TCTGAATTCT TCCACAGACC CATTTAACAG ACAGACACTT 
ACAGAAAATA TGCTTGAACC AGTGCCAGAA CTGAAGGAAA AAATCCAGGC TTGGATGCGT 
GACAAGCAAA ACGCAGATCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916084.1
CDS	320..3823
Translation	MEELSADEIRRRRLARLAGGPSSQPTTPLTSPQRENPPGPPAGAPTPGVSHQLGLNVQGITPATSPTGATGVAHRSHSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDSAAGEKSMSQVDVDSGIENMEVDENDRREKRSLPDKEPNLGSEVSEEQTLQLVCKIFRVSWKDRDRDVIFLQSLSSQFSQNPKKVYSNYKDLISQILMEVLMMSTHSKDDNPFASLTATSQPITVAQIPNRSLMLNSASKPDTSPMFCNSGSFSASSLSSATSSTESCADHFTIETCKETEMLNYLIECFDRVGIEERKAPKMCSQPDVSQLLSNIRLQCICHAALVLQGALTQPRSLQQPSLVVPYMLCRNLPFGFIQELVRTTYQDEEVFKHIFVPILQGLSQAATECSLDSDNFKYPLMALGELCEIKFGKTHPVCCLITSLPLWLPKPLSPASGRELQRLSFLGSYFSLSVFAEDDNKVVEKYFSGPAITLENTRVVSQSLQHYLESARQELFKIIHSILLNGETREAALNYMAALVNANIKKAQIQTDDRLVSTDGFMLNFLWVLQQLSSKIKLETVDPMYIFHPKCRILLPSDETRIKASTEEVTSWMSDISNDPSLFSEPKFPTECFFLTLHAHHLAILPSCRRYIRRLRAIRELNRTVEELKNNESQWKDSPLAARHREMLKRCKTQLKKLVRCKACADTGLLDESFLRRCLNFYGMVIQFLLRIMDPSYPNITMPLNLDIPKMFATLPEFYVEDVAEFLFFIVQYAPQVLFEPCTQDVATFLVVLLCNQNYIRNPYLVAKLVEVMFVTNPAIQHRTQKFFEMIESHPLSTKLLVPSLMKFYTDVEHTGSTSEFYDKFTIRYHISTIFKSLWQNMAHHSTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEMQEEMRNKEHWEQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLHLDCARFAKAIADDQRSYSKELFEEVISKMKKAGIKSTIAIEKFKLLAEKVEEIVARNARAEIDYSDAPDEFRDPLMDTLMTEPVRLPSGTIMDRSIILRHLLNSSTDPFNRQTLTENMLEPVPELKEKIQAWMRDKQNADH

Target ORF information:

RefSeq Version	XM_004916027.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916027.4

ORF Insert Sequence:

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTGCT ACTAGCAGTA CAGAATCCTG TGCTGATCAC 
TTCACTATTG AAACTTGCAA AGAAACAGAG ATGCTCAACT ACCTCATTGA ATGCTTCGAT 
AGAGTGGGAA TTGAAGAGAG GAAAGCACCA AAGATGTGCA GCCAGCCAGA TGTGAGTCAA 
CTCCTGAGCA ATATTCGATT ACAGTGTATT TGCCATGCTG CTCTTGTGTT ACAGGGGGCC 
CTCACACAGC CAAGGTCTTT GCAACAGCCA TCTTTGGTGG TACCATACAT GCTCTGCAGA 
AACCTACCTT TTGGGTTTAT TCAAGAGTTA GTAAGGACTA CATACCAAGA TGAAGAAGTG 
TTTAAGCATA TCTTTGTTCC CATTCTTCAA GGCTTATCTC AAGCTGCTAC AGAGTGTTCT 
CTGGACAGTG ATAATTTTAA ATACCCTCTA ATGGCATTGG GTGAGCTTTG TGAAATTAAG 
TTTGGGAAGA CTCATCCTGT CTGCTGTTTG ATAACCTCTT TGCCCTTATG GTTGCCGAAG 
CCACTGAGCC CTGCAAGTGG ACGTGAACTT CAACGACTTT CATTTCTTGG TTCATATTTT 
AGTCTCTCCG TCTTTGCTGA AGATGATAAT AAAGTAGTCG AAAAATATTT TTCTGGACCA 
GCTATAACTT TGGAAAATAC CCGCGTAGTC AGCCAATCTT TGCAACATTA CTTGGAGTCT 
GCCAGACAAG AATTGTTCAA GATTATACAC AGTATATTGC TAAATGGAGA AACCAGAGAA 
GCTGCCCTTA ACTACATGGC TGCTCTTGTC AATGCCAACA TCAAAAAAGC ACAGATTCAG 
ACAGATGATA GGCTGGTTTC TACAGATGGC TTCATGCTTA ATTTCCTTTG GGTGCTGCAG 
CAGCTCAGCT CTAAGATTAA ACTGGAGACT GTTGATCCTA TGTATATTTT CCACCCAAAG 
TGCCGCATCC TCTTACCCAG TGATGAAACT CGCATCAAAG CTTCAACTGA GGAGGTGACT 
AGCTGGATGT CTGATATAAG TAATGATCCA TCTTTATTCT CAGAACCCAA GTTTCCAACA 
GAGTGCTTTT TCCTGACTCT ACACGCCCAT CATCTAGCCA TCCTACCTAG CTGCCGTCGC 
TACATCCGCC GCTTACGGGC CATCAGAGAG CTTAACAGGA CTGTTGAAGA GCTTAAGAAT 
AATGAAAGTC AGTGGAAAGA TTCCCCATTA GCTGCAAGAC ACAGAGAAAT GTTAAAGCGC 
TGCAAAACTC AACTCAAGAA GCTGGTGCGC TGCAAAGCCT GTGCGGATAC TGGTTTATTG 
GATGAAAGTT TTCTCCGGAG ATGTTTAAAT TTTTATGGAA TGGTGATCCA GTTCCTACTA 
CGTATCATGG ATCCTTCATA CCCAAACATA ACAATGCCTT TAAATCTGGA TATACCCAAA 
ATGTTTGCAA CGCTGCCTGA GTTTTATGTA GAAGATGTTG CTGAATTTTT GTTTTTTATT 
GTGCAATATG CTCCTCAGGT TCTTTTTGAA CCCTGCACGC AAGATGTTGC AACTTTCTTA 
GTCGTGCTGC TGTGCAATCA GAACTATATA CGAAATCCTT ACCTTGTGGC CAAGCTAGTA 
GAAGTCATGT TTGTGACCAA TCCTGCCATC CAGCACAGAA CTCAAAAGTT CTTTGAAATG 
ATTGAAAGTC ACCCTTTGTC AACTAAACTT TTAGTCCCCT CACTGATGAA ATTTTACACA 
GATGTGGAGC ATACAGGTTC CACAAGTGAG TTCTATGATA AGTTCACCAT TCGTTACCAC 
ATCAGCACTA TTTTTAAAAG CCTGTGGCAG AATATGGCTC ATCACAGCAC TTTCATGGAG 
GAGTTCAACT CTGGCAAGCA GTTTGTACGT TATATAAACA TGCTCATAAA TGATACTACA 
TTTCTCCTGG ATGAGAGTCT AGAATCGCTT AAACGCATCC ATGAAATGCA GGAGGAGATG 
AGAAACAAAG AACATTGGGA ACAGTTGCCA AGGGATCAGC AGCAAGCACG GCAGTCACAG 
CTTGCACAGG ATGAGCGTGT TTCTCGTTCA TATCTTGCTC TAGCAACTGA GACTGTAGAT 
ATGTTTCACA TCCTTACTAA ACAAGTGCAG AAACCTTTCT TAAGACCTGA ACTAGGGCCC 
AGGCTAGCAG CAATGTTAAA TTTTAACCTC CAGCAATTGT GTGGACCAAA ATGTCGAGAC 
CTGAAGGTCG AGAACCCAGA GAAATATGGT TTTGAACCAA AGAAACTTCT GGACCAGCTC 
ACAGATATCT ATTTGCATTT AGATTGTGCT CGGTTTGCAA AAGCTATTGC TGATGATCAG 
AGATCATACA GCAAGGAACT TTTTGAAGAA GTTATTTCAA AAATGAAGAA AGCTGGAATT 
AAGTCTACAA TTGCCATAGA AAAATTTAAG CTGCTTGCAG AGAAAGTTGA AGAGATTGTG 
GCCAGAAATG CTCGTGCAGA GATAGATTAC AGTGATGCGC CAGATGAATT TAGAGATCCT 
CTCATGGACA CACTGATGAC AGAACCAGTA AGACTTCCAT CTGGGACCAT CATGGATCGA 
TCTATCATTT TAAGGCATCT TCTGAATTCT TCCACAGACC CATTTAACAG ACAGACACTT 
ACAGAAAATA TGCTTGAACC AGTGCCAGAA CTGAAGGAAA AAATCCAGGC TTGGATGCGT 
GACAAGCAAA ACGCAGATCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ube4b ( XM_004916027.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916084.1 Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X3, mRNA.

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CloneID	OXa12996
Clone ID Related Accession (Same CDS sequence)	XM_004916027.3
Accession Version	XM_004916027.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin conjugation factor E4 B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTGCT ACTAGCAGTA CAGAATCCTG TGCTGATCAC 
TTCACTATTG AAACTTGCAA AGAAACAGAG ATGCTCAACT ACCTCATTGA ATGCTTCGAT 
AGAGTGGGAA TTGAAGAGAG GAAAGCACCA AAGATGTGCA GCCAGCCAGA TGTGAGTCAA 
CTCCTGAGCA ATATTCGATT ACAGTGTATT TGCCATGCTG CTCTTGTGTT ACAGGGGGCC 
CTCACACAGC CAAGGTCTTT GCAACAGCCA TCTTTGGTGG TACCATACAT GCTCTGCAGA 
AACCTACCTT TTGGGTTTAT TCAAGAGTTA GTAAGGACTA CATACCAAGA TGAAGAAGTG 
TTTAAGCATA TCTTTGTTCC CATTCTTCAA GGCTTATCTC AAGCTGCTAC AGAGTGTTCT 
CTGGACAGTG ATAATTTTAA ATACCCTCTA ATGGCATTGG GTGAGCTTTG TGAAATTAAG 
TTTGGGAAGA CTCATCCTGT CTGCTGTTTG ATAACCTCTT TGCCCTTATG GTTGCCGAAG 
CCACTGAGCC CTGCAAGTGG ACGTGAACTT CAACGACTTT CATTTCTTGG TTCATATTTT 
AGTCTCTCCG TCTTTGCTGA AGATGATAAT AAAGTAGTCG AAAAATATTT TTCTGGACCA 
GCTATAACTT TGGAAAATAC CCGCGTAGTC AGCCAATCTT TGCAACATTA CTTGGAGTCT 
GCCAGACAAG AATTGTTCAA GATTATACAC AGTATATTGC TAAATGGAGA AACCAGAGAA 
GCTGCCCTTA ACTACATGGC TGCTCTTGTC AATGCCAACA TCAAAAAAGC ACAGATTCAG 
ACAGATGATA GGCTGGTTTC TACAGATGGC TTCATGCTTA ATTTCCTTTG GGTGCTGCAG 
CAGCTCAGCT CTAAGATTAA ACTGGAGACT GTTGATCCTA TGTATATTTT CCACCCAAAG 
TGCCGCATCC TCTTACCCAG TGATGAAACT CGCATCAAAG CTTCAACTGA GGAGGTGACT 
AGCTGGATGT CTGATATAAG TAATGATCCA TCTTTATTCT CAGAACCCAA GTTTCCAACA 
GAGTGCTTTT TCCTGACTCT TCACGCCCAT CATCTAGCCA TCCTACCTAG CTGCCGTCGC 
TACATCCGCC GCTTACGGGC CATCAGAGAG CTTAACAGGA CTGTTGAAGA GCTTAAGAAT 
AATGAAAGTC AGTGGAAAGA TTCCCCATTA GCTGCAAGAC ACAGAGAAAT GTTAAAGCGC 
TGCAAAACTC AACTCAAGAA GCTGGTGCGC TGCAAAGCCT GTGCGGATAC TGGTTTATTG 
GATGAAAGTT TTCTCCGGAG ATGTTTAAAT TTTTATGGAA TGGTGATCCA GTTCCTACTA 
CGTATCATGG ATCCTTCATA CCCAAACATA ACAATGCCTT TAAATCTGGA TATACCCAAA 
ATGTTTGCAA CGCTGCCTGA GTTTTATGTA GAAGATGTTG CTGAATTTTT GTTTTTTATT 
GTGCAATATG CTCCTCAGGT TCTTTTTGAA CCCTGCACGC AAGATGTTGC AACTTTCTTA 
GTCGTGCTGC TGTGCAATCA GAACTATATA CGAAATCCTT ACCTTGTGGC CAAGCTAGTA 
GAAGTCATGT TTGTGACCAA TCCTGCCATC CAGCACAGAA CTCAAAAGTT CTTTGAAATG 
ATTGAAAGTC ACCCTTTGTC AACTAAACTT TTAGTCCCCT CACTGATGAA ATTTTACACA 
GATGTGGAGC ATACAGGTTC CACAAGTGAG TTCTATGATA AGTTCACCAT TCGTTACCAC 
ATCAGCACTA TTTTTAAAAG CCTGTGGCAG AATATGGCTC ATCACAGCAC TTTCATGGAG 
GAGTTCAACT CTGGCAAGCA GTTTGTACGT TATATAAACA TGCTCATAAA TGATACTACA 
TTTCTCCTGG ATGAGAGTCT AGAATCGCTT AAACGCATCC ATGAAATGCA GGAGGAGATG 
AGAAACAAAG AACATTGGGA ACAGTTGCCA AGGGATCAGC AGCAAGCACG GCAGTCACAG 
CTTGCACAGG ATGAGCGTGT TTCTCGTTCA TATCTTGCTC TAGCAACTGA GACTGTAGAT 
ATGTTTCACA TCCTTACTAA ACAAGTGCAG AAACCTTTCT TAAGACCTGA ACTAGGGCCC 
AGGCTAGCAG CAATGTTAAA TTTTAACCTC CAGCAATTGT GTGGACCAAA ATGTCGAGAC 
CTGAAGGTCG AGAACCCAGA GAAATATGGT TTTGAACCAA AGAAACTTCT GGACCAGCTC 
ACAGATATCT ATTTGCATTT AGATTGTGCT CGGTTTGCAA AAGCTATTGC TGATGATCAG 
AGATCATACA GCAAGGAACT TTTTGAAGAA GTTATTTCAA AAATGAAGAA AGCTGGAATT 
AAGTCTACAA TTGCCATAGA AAAATTTAAG CTGCTTGCAG AGAAAGTTGA AGAGATTGTG 
GCCAGAAATG CTCGTGCAGA GATAGATTAC AGTGATGCGC CAGATGAATT TAGAGATCCT 
CTCATGGACA CACTGATGAC AGAACCAGTA AGACTTCCAT CTGGGACCAT CATGGATCGA 
TCTATCATTT TAAGGCATCT TCTGAATTCT TCCACAGACC CATTTAACAG ACAGACACTT 
ACAGAAAATA TGCTTGAACC AGTGCCAGAA CTGAAGGAAA AAATCCAGGC TTGGATGCGT 
GACAAGCAAA ACGCAGATCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916084.1
CDS	327..3830
Translation	MEELSADEIRRRRLARLAGGPSSQPTTPLTSPQRENPPGPPAGAPTPGVSHQLGLNVQGITPATSPTGATGVAHRSHSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDSAAGEKSMSQVDVDSGIENMEVDENDRREKRSLPDKEPNLGSEVSEEQTLQLVCKIFRVSWKDRDRDVIFLQSLSSQFSQNPKKVYSNYKDLISQILMEVLMMSTHSKDDNPFASLTATSQPITVAQIPNRSLMLNSASKPDTSPMFCNSGSFSASSLSSATSSTESCADHFTIETCKETEMLNYLIECFDRVGIEERKAPKMCSQPDVSQLLSNIRLQCICHAALVLQGALTQPRSLQQPSLVVPYMLCRNLPFGFIQELVRTTYQDEEVFKHIFVPILQGLSQAATECSLDSDNFKYPLMALGELCEIKFGKTHPVCCLITSLPLWLPKPLSPASGRELQRLSFLGSYFSLSVFAEDDNKVVEKYFSGPAITLENTRVVSQSLQHYLESARQELFKIIHSILLNGETREAALNYMAALVNANIKKAQIQTDDRLVSTDGFMLNFLWVLQQLSSKIKLETVDPMYIFHPKCRILLPSDETRIKASTEEVTSWMSDISNDPSLFSEPKFPTECFFLTLHAHHLAILPSCRRYIRRLRAIRELNRTVEELKNNESQWKDSPLAARHREMLKRCKTQLKKLVRCKACADTGLLDESFLRRCLNFYGMVIQFLLRIMDPSYPNITMPLNLDIPKMFATLPEFYVEDVAEFLFFIVQYAPQVLFEPCTQDVATFLVVLLCNQNYIRNPYLVAKLVEVMFVTNPAIQHRTQKFFEMIESHPLSTKLLVPSLMKFYTDVEHTGSTSEFYDKFTIRYHISTIFKSLWQNMAHHSTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEMQEEMRNKEHWEQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLHLDCARFAKAIADDQRSYSKELFEEVISKMKKAGIKSTIAIEKFKLLAEKVEEIVARNARAEIDYSDAPDEFRDPLMDTLMTEPVRLPSGTIMDRSIILRHLLNSSTDPFNRQTLTENMLEPVPELKEKIQAWMRDKQNADH

Target ORF information:

RefSeq Version	XM_004916027.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitination factor E4B (ube4b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916027.3

ORF Insert Sequence:

ATGGAGGAGC TAAGCGCTGA CGAGATTAGA CGAAGGCGCC TTGCTCGACT TGCTGGTGGG 
CCATCTTCCC AGCCCACAAC TCCTCTTACT TCTCCACAAA GAGAGAATCC TCCAGGACCA 
CCAGCAGGAG CACCAACACC TGGTGTGTCC CATCAGCTTG GTCTAAATGT ACAGGGCATA 
ACCCCAGCTA CCTCACCTAC AGGAGCAACA GGGGTTGCCC ATAGAAGTCA TAGCAGTGAA 
GGAGTCAGTT CATTAAGCAG TTCACCATCA AATAGCCTTG AAACTCAGTC ACAGTCTCTT 
TCTCGGTCAC AAAGCATGGA CATTGACAGT GCAGCAGGTG AAAAAAGCAT GTCCCAGGTG 
GATGTGGACT CAGGAATTGA GAACATGGAG GTTGATGAAA ATGACCGCAG GGAGAAAAGA 
AGCCTTCCAG ATAAGGAGCC CAATTTAGGG TCTGAGGTAT CAGAGGAACA AACTTTGCAG 
CTGGTCTGTA AGATCTTCAG AGTTTCCTGG AAGGACAGAG ATAGAGATGT AATCTTTCTG 
CAGTCTCTCT CATCTCAGTT CAGTCAGAAT CCAAAAAAAG TGTACTCAAA CTATAAGGAT 
CTAATTAGTC AGATTTTAAT GGAAGTCTTG ATGATGTCCA CTCATTCTAA AGATGACAAC 
CCCTTTGCCA GCTTAACTGC CACATCGCAG CCAATTACAG TTGCACAGAT ACCTAACAGG 
AGTTTGATGT TAAACTCTGC ATCCAAACCC GACACTAGTC CTATGTTCTG TAACTCTGGA 
TCATTCAGTG CCAGCTCTCT ATCTAGTGCT ACTAGCAGTA CAGAATCCTG TGCTGATCAC 
TTCACTATTG AAACTTGCAA AGAAACAGAG ATGCTCAACT ACCTCATTGA ATGCTTCGAT 
AGAGTGGGAA TTGAAGAGAG GAAAGCACCA AAGATGTGCA GCCAGCCAGA TGTGAGTCAA 
CTCCTGAGCA ATATTCGATT ACAGTGTATT TGCCATGCTG CTCTTGTGTT ACAGGGGGCC 
CTCACACAGC CAAGGTCTTT GCAACAGCCA TCTTTGGTGG TACCATACAT GCTCTGCAGA 
AACCTACCTT TTGGGTTTAT TCAAGAGTTA GTAAGGACTA CATACCAAGA TGAAGAAGTG 
TTTAAGCATA TCTTTGTTCC CATTCTTCAA GGCTTATCTC AAGCTGCTAC AGAGTGTTCT 
CTGGACAGTG ATAATTTTAA ATACCCTCTA ATGGCATTGG GTGAGCTTTG TGAAATTAAG 
TTTGGGAAGA CTCATCCTGT CTGCTGTTTG ATAACCTCTT TGCCCTTATG GTTGCCGAAG 
CCACTGAGCC CTGCAAGTGG ACGTGAACTT CAACGACTTT CATTTCTTGG TTCATATTTT 
AGTCTCTCCG TCTTTGCTGA AGATGATAAT AAAGTAGTCG AAAAATATTT TTCTGGACCA 
GCTATAACTT TGGAAAATAC CCGCGTAGTC AGCCAATCTT TGCAACATTA CTTGGAGTCT 
GCCAGACAAG AATTGTTCAA GATTATACAC AGTATATTGC TAAATGGAGA AACCAGAGAA 
GCTGCCCTTA ACTACATGGC TGCTCTTGTC AATGCCAACA TCAAAAAAGC ACAGATTCAG 
ACAGATGATA GGCTGGTTTC TACAGATGGC TTCATGCTTA ATTTCCTTTG GGTGCTGCAG 
CAGCTCAGCT CTAAGATTAA ACTGGAGACT GTTGATCCTA TGTATATTTT CCACCCAAAG 
TGCCGCATCC TCTTACCCAG TGATGAAACT CGCATCAAAG CTTCAACTGA GGAGGTGACT 
AGCTGGATGT CTGATATAAG TAATGATCCA TCTTTATTCT CAGAACCCAA GTTTCCAACA 
GAGTGCTTTT TCCTGACTCT TCACGCCCAT CATCTAGCCA TCCTACCTAG CTGCCGTCGC 
TACATCCGCC GCTTACGGGC CATCAGAGAG CTTAACAGGA CTGTTGAAGA GCTTAAGAAT 
AATGAAAGTC AGTGGAAAGA TTCCCCATTA GCTGCAAGAC ACAGAGAAAT GTTAAAGCGC 
TGCAAAACTC AACTCAAGAA GCTGGTGCGC TGCAAAGCCT GTGCGGATAC TGGTTTATTG 
GATGAAAGTT TTCTCCGGAG ATGTTTAAAT TTTTATGGAA TGGTGATCCA GTTCCTACTA 
CGTATCATGG ATCCTTCATA CCCAAACATA ACAATGCCTT TAAATCTGGA TATACCCAAA 
ATGTTTGCAA CGCTGCCTGA GTTTTATGTA GAAGATGTTG CTGAATTTTT GTTTTTTATT 
GTGCAATATG CTCCTCAGGT TCTTTTTGAA CCCTGCACGC AAGATGTTGC AACTTTCTTA 
GTCGTGCTGC TGTGCAATCA GAACTATATA CGAAATCCTT ACCTTGTGGC CAAGCTAGTA 
GAAGTCATGT TTGTGACCAA TCCTGCCATC CAGCACAGAA CTCAAAAGTT CTTTGAAATG 
ATTGAAAGTC ACCCTTTGTC AACTAAACTT TTAGTCCCCT CACTGATGAA ATTTTACACA 
GATGTGGAGC ATACAGGTTC CACAAGTGAG TTCTATGATA AGTTCACCAT TCGTTACCAC 
ATCAGCACTA TTTTTAAAAG CCTGTGGCAG AATATGGCTC ATCACAGCAC TTTCATGGAG 
GAGTTCAACT CTGGCAAGCA GTTTGTACGT TATATAAACA TGCTCATAAA TGATACTACA 
TTTCTCCTGG ATGAGAGTCT AGAATCGCTT AAACGCATCC ATGAAATGCA GGAGGAGATG 
AGAAACAAAG AACATTGGGA ACAGTTGCCA AGGGATCAGC AGCAAGCACG GCAGTCACAG 
CTTGCACAGG ATGAGCGTGT TTCTCGTTCA TATCTTGCTC TAGCAACTGA GACTGTAGAT 
ATGTTTCACA TCCTTACTAA ACAAGTGCAG AAACCTTTCT TAAGACCTGA ACTAGGGCCC 
AGGCTAGCAG CAATGTTAAA TTTTAACCTC CAGCAATTGT GTGGACCAAA ATGTCGAGAC 
CTGAAGGTCG AGAACCCAGA GAAATATGGT TTTGAACCAA AGAAACTTCT GGACCAGCTC 
ACAGATATCT ATTTGCATTT AGATTGTGCT CGGTTTGCAA AAGCTATTGC TGATGATCAG 
AGATCATACA GCAAGGAACT TTTTGAAGAA GTTATTTCAA AAATGAAGAA AGCTGGAATT 
AAGTCTACAA TTGCCATAGA AAAATTTAAG CTGCTTGCAG AGAAAGTTGA AGAGATTGTG 
GCCAGAAATG CTCGTGCAGA GATAGATTAC AGTGATGCGC CAGATGAATT TAGAGATCCT 
CTCATGGACA CACTGATGAC AGAACCAGTA AGACTTCCAT CTGGGACCAT CATGGATCGA 
TCTATCATTT TAAGGCATCT TCTGAATTCT TCCACAGACC CATTTAACAG ACAGACACTT 
ACAGAAAATA TGCTTGAACC AGTGCCAGAA CTGAAGGAAA AAATCCAGGC TTGGATGCGT 
GACAAGCAAA ACGCAGATCA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ube4b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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