arhgef10 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following arhgef10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa44777 XM_018094426.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa58472 XM_002935157.5
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa58472 XM_031902163.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa58472 XM_018094424.2
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa58472 XM_012963478.3
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa58472 XM_012963476.3
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa58473 XM_012963479.3
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa10351 XM_002935157.4
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa10351 XM_012963478.2
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa10351 XM_018094424.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa10351 XM_012963476.2
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa10351 XM_018094425.1
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa34836 XM_012963479.2
Latest version!
Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa44777
    Clone ID Related Accession (Same CDS sequence) XM_018094426.1
    Accession Version XM_018094426.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TATGCAAAGG CTGGTAAAGG CTGCTAAAGA GGGGACAAAA
    GATGGACTTG AAAAAACCAA GGCTGCTGTG AAGAAAGGTC GTTCCTACAT AAGGACCAAA
    TCATTTGTCT TGCATGATCG CAAATCCACC AGTCTTGAAG ATGACGAGTT GAATTTATTT
    ATTGATGTGG ACTGCATGCA CATAGAGGCG ATCATGTCTC CAATGCCAGA AGGACTATCA
    CAACAGCAGG TTGTCAGACG CTACATTCTA GGATCAATTG TGGAAAGTGA GAAGAACTAT
    GTAGACTCAT TAAAAAGAAT TCTCGAGCAA TATGAGAAGC CATTGTGGGA GACAGAGCCA
    AGGTTATTAA GTGAAAGAAA GCTGAAAATA GTCTTCTATC GCATAAAGGA AATTCTCCAG
    TGTCACTCAC TGTTCCAGAT TGCTCTCGCC AGCCGAGTGG CAGAGTGGGA TACTGCCGAA
    ATGATTGGGG ATGTGTTTGT AGCTTCATTT TCAAAATCCA TGGTCCTGGA TGCATACAGT
    GAGTATGTGA ATAATTTCAG TACAGCCGTC GGAGTTCTCA GGAAAGCCTG TGCCACAAAA
    CCATCTTTTT TGGAGTTTTT AAAACAGTGC CAAGAATCCA GTCCTGACCG AATAACTCTC
    TATGGTCTGA TGATGAAACC TATTCAGCGC TTCCCACAGT TTATACTTCT GCTTCAGGAC
    ATGCTAAAAA ATACAAACAA GGGGCACCCA GACCGGCTCC CTCTACAGAT GGCTCTAACA
    GAGCTGGAAA CCTTAGCGGA CAAACTGAAC GAAAGGAAAC GAGATGCAGA TCAGCGCTGT
    GAAATTAAGC AGATTGCAAA GGCAATGAAT GAAAGATATC TGAATAAGCT CCTAAGTAAT
    GGTAGCAGAT ACCTTGTTCG ATCAGATGAT ATGGTTGAAA CCGTTTTCAA TGAAAGAGGG
    GAGATAATTA AAACAAAGCA GCGCCGGCTT TTCATGTTAA ATGATGTTTT GATGTGTGCC
    ACGGCAAATG CTAGGTCTTC TGTGGAAGGC AGTAGCGCAG TAACCACAAA CCAAAAGTAC
    CTACTAAAAT GGAGTGTACC ACTAAGCCAT GCAGAGGTAA TTGAATATGG AGAAAGTGAG
    GACCAAGGAG ACCAAACTCT GTATTCTTCT TTGCATGTTG CTGAAAAGGT TGTTGTTAGC
    AGTAAACCAA ATAAACTGTA CATGGGGCCA GGGCAGCTGT ATAATGACTT GCAGAACCTA
    CTTCATGATT TGGATGTAGT CAACCAGATA TCTCAGCTTG TTGGACAGCT GAAGGGAAAC
    TATCAGAACC TCAACCATGC AGTTGCCCAA GACTGGTCTG TAGGGTTACA AAAGTTGATC
    TTGAAGAAAG AAAATGAAAT CCGAGCTGCA GACCGCTGCA GAATTCAGCT CCAACTCCCT
    GGCAAACAGG ACAAGTCTGG ACGCCCCACT TACTTTACTG CAGTATTTAA CACATTCTCT
    CCTGCTGCCA AACAGTCATG GCTCAGTAAC TTACAGATGG CTAAGCTTGC TCTTGGAGAG
    GAGAACAACA CAGGATGGTT CTGTCTAGAA GATGAAGGCC ATCAAGTTAA AAAGGAGAAG
    CATCCCTTGC TTGTTAAACA CTTACCTGTT GTCATGTCAA AGCAGCAAGA ATTTAAGATT
    GAATGTGCAG TCTATAATCC TGAACCATAT CTCAGTAGTG AAAATGATCC AGATACACTT
    TCCATGCATC ATGGCTTTCT ATGGATTGGC AGCTGCACTA ATCAGATGGG GCAGATTTCA
    GTTCTTTCCC TCCATAATCG TAGCCCTAAG GTGTTGGAGT GTTTCAATGT CGAATCACGC
    ATACTCTGCA TGGTCTATAT CCCAGAGGAA GCAAAACAGA AACAACTAAG CGATGCCCCA
    GATTTGGAAA ATGGGTCTTC CCAAGTACCA GATGTGCCCA CTATTTGTCT GGGCATGGAA
    GAAGGAAGCA TATGTGTCTA CAAAAGTAGT CAAGGATCTA AAAAAGTACG GTTGCAGCAC
    TTTTTCACAC CAGAAAAATC AACTGTTATG AGCCTTGCTA GCATCTCTCA GTGCTTGTAT
    GCAGGTCTTG TAAATGGAAA TCTCGCCACC TACACAAAAG CTGAAGATGG AAACTGGAAC
    ACAGACCAAC CAAAGCTAAT CAAATTAGGA GTTTTGCCGG TTCGAAGTCT GCTGATAATG
    GAGAGAACAA TCTGGGCAGC TTCTGGAGAG CAGATATTTA TTATTGGCCA GGATTCTAAT
    GCCATTGAGC ACCAGTTTGA GGCCCATAGT GAAGAAGGCA TGGTGATTTC TCACATGGCA
    GTAGCGGGTG TTGGAATTTG GATTGCATTC ACTACTGGAT CAACTCTGCG GCTCTTTCAC
    ACCGAGACAT TTGAACATTT GCAAGATATA AATATAGCAA CTCCAATACA CAACATGTTA
    CCAGGACAGC ATCGGGTGTC TGTAACTAGT TTGCTGGTTT GTCACGGTTT GCTAATGGTT
    GGCACAAATC TGGGAGTTAT AGTTTCATTG CCAGTTCCTC GTTTACAAGG AATCCCAAAA
    GTGACCGGAA GAGGCATGGT CTCGTATCAC GCTCATCACA GTCCAGTTAA ATTCCTGGTC
    AAGGCAACTG CCATTAATAA GAAATACAGA CGCACACCAA GCAGATGTAG CCAGATTTCT
    CACTCGGAGT TAAAGAATGA AGACTCACAC TGTACTGGTG TTCAGCAAAA CGATGAAGCC
    GCTGCATCAG CTACAAACAA TAACAATGAC AGTGCTGTGT GGCTTGGAGG GTCTATGGGG
    TCCATTGCCC AGAAAAGTGA CTTATCCTCT TCATCGGGGT CCCTAACACT GTCTCATGGA
    TCCAACTCCT GTGACGCAGG AAAGGAAGAC TGCACTCTGT CCGATCTTTT GGGTGATCCA
    AGCATGCCAC TGCCAAATAG CAAACAACAA AAAGCCAAAA GACTCAAAGC AGACTCTGTC
    CTAGTGATCT CTGGTGGCCA GGGCCACAGA AAGTTAAACA AAAAGGTCAA ACCACAGCGA
    CAAGAAGAGC TGGTATCTTC TGCAATGGTT TGGCAGATCC CACTTAACAT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949915.1
    CDS514..4527
    Translation

    Target ORF information:

    RefSeq Version XM_018094426.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094426.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TATGCAAAGG CTGGTAAAGG CTGCTAAAGA GGGGACAAAA
    GATGGACTTG AAAAAACCAA GGCTGCTGTG AAGAAAGGTC GTTCCTACAT AAGGACCAAA
    TCATTTGTCT TGCATGATCG CAAATCCACC AGTCTTGAAG ATGACGAGTT GAATTTATTT
    ATTGATGTGG ACTGCATGCA CATAGAGGCG ATCATGTCTC CAATGCCAGA AGGACTATCA
    CAACAGCAGG TTGTCAGACG CTACATTCTA GGATCAATTG TGGAAAGTGA GAAGAACTAT
    GTAGACTCAT TAAAAAGAAT TCTCGAGCAA TATGAGAAGC CATTGTGGGA GACAGAGCCA
    AGGTTATTAA GTGAAAGAAA GCTGAAAATA GTCTTCTATC GCATAAAGGA AATTCTCCAG
    TGTCACTCAC TGTTCCAGAT TGCTCTCGCC AGCCGAGTGG CAGAGTGGGA TACTGCCGAA
    ATGATTGGGG ATGTGTTTGT AGCTTCATTT TCAAAATCCA TGGTCCTGGA TGCATACAGT
    GAGTATGTGA ATAATTTCAG TACAGCCGTC GGAGTTCTCA GGAAAGCCTG TGCCACAAAA
    CCATCTTTTT TGGAGTTTTT AAAACAGTGC CAAGAATCCA GTCCTGACCG AATAACTCTC
    TATGGTCTGA TGATGAAACC TATTCAGCGC TTCCCACAGT TTATACTTCT GCTTCAGGAC
    ATGCTAAAAA ATACAAACAA GGGGCACCCA GACCGGCTCC CTCTACAGAT GGCTCTAACA
    GAGCTGGAAA CCTTAGCGGA CAAACTGAAC GAAAGGAAAC GAGATGCAGA TCAGCGCTGT
    GAAATTAAGC AGATTGCAAA GGCAATGAAT GAAAGATATC TGAATAAGCT CCTAAGTAAT
    GGTAGCAGAT ACCTTGTTCG ATCAGATGAT ATGGTTGAAA CCGTTTTCAA TGAAAGAGGG
    GAGATAATTA AAACAAAGCA GCGCCGGCTT TTCATGTTAA ATGATGTTTT GATGTGTGCC
    ACGGCAAATG CTAGGTCTTC TGTGGAAGGC AGTAGCGCAG TAACCACAAA CCAAAAGTAC
    CTACTAAAAT GGAGTGTACC ACTAAGCCAT GCAGAGGTAA TTGAATATGG AGAAAGTGAG
    GACCAAGGAG ACCAAACTCT GTATTCTTCT TTGCATGTTG CTGAAAAGGT TGTTGTTAGC
    AGTAAACCAA ATAAACTGTA CATGGGGCCA GGGCAGCTGT ATAATGACTT GCAGAACCTA
    CTTCATGATT TGGATGTAGT CAACCAGATA TCTCAGCTTG TTGGACAGCT GAAGGGAAAC
    TATCAGAACC TCAACCATGC AGTTGCCCAA GACTGGTCTG TAGGGTTACA AAAGTTGATC
    TTGAAGAAAG AAAATGAAAT CCGAGCTGCA GACCGCTGCA GAATTCAGCT CCAACTCCCT
    GGCAAACAGG ACAAGTCTGG ACGCCCCACT TACTTTACTG CAGTATTTAA CACATTCTCT
    CCTGCTGCCA AACAGTCATG GCTCAGTAAC TTACAGATGG CTAAGCTTGC TCTTGGAGAG
    GAGAACAACA CAGGATGGTT CTGTCTAGAA GATGAAGGCC ATCAAGTTAA AAAGGAGAAG
    CATCCCTTGC TTGTTAAACA CTTACCTGTT GTCATGTCAA AGCAGCAAGA ATTTAAGATT
    GAATGTGCAG TCTATAATCC TGAACCATAT CTCAGTAGTG AAAATGATCC AGATACACTT
    TCCATGCATC ATGGCTTTCT ATGGATTGGC AGCTGCACTA ATCAGATGGG GCAGATTTCA
    GTTCTTTCCC TCCATAATCG TAGCCCTAAG GTGTTGGAGT GTTTCAATGT CGAATCACGC
    ATACTCTGCA TGGTCTATAT CCCAGAGGAA GCAAAACAGA AACAACTAAG CGATGCCCCA
    GATTTGGAAA ATGGGTCTTC CCAAGTACCA GATGTGCCCA CTATTTGTCT GGGCATGGAA
    GAAGGAAGCA TATGTGTCTA CAAAAGTAGT CAAGGATCTA AAAAAGTACG GTTGCAGCAC
    TTTTTCACAC CAGAAAAATC AACTGTTATG AGCCTTGCTA GCATCTCTCA GTGCTTGTAT
    GCAGGTCTTG TAAATGGAAA TCTCGCCACC TACACAAAAG CTGAAGATGG AAACTGGAAC
    ACAGACCAAC CAAAGCTAAT CAAATTAGGA GTTTTGCCGG TTCGAAGTCT GCTGATAATG
    GAGAGAACAA TCTGGGCAGC TTCTGGAGAG CAGATATTTA TTATTGGCCA GGATTCTAAT
    GCCATTGAGC ACCAGTTTGA GGCCCATAGT GAAGAAGGCA TGGTGATTTC TCACATGGCA
    GTAGCGGGTG TTGGAATTTG GATTGCATTC ACTACTGGAT CAACTCTGCG GCTCTTTCAC
    ACCGAGACAT TTGAACATTT GCAAGATATA AATATAGCAA CTCCAATACA CAACATGTTA
    CCAGGACAGC ATCGGGTGTC TGTAACTAGT TTGCTGGTTT GTCACGGTTT GCTAATGGTT
    GGCACAAATC TGGGAGTTAT AGTTTCATTG CCAGTTCCTC GTTTACAAGG AATCCCAAAA
    GTGACCGGAA GAGGCATGGT CTCGTATCAC GCTCATCACA GTCCAGTTAA ATTCCTGGTC
    AAGGCAACTG CCATTAATAA GAAATACAGA CGCACACCAA GCAGATGTAG CCAGATTTCT
    CACTCGGAGT TAAAGAATGA AGACTCACAC TGTACTGGTG TTCAGCAAAA CGATGAAGCC
    GCTGCATCAG CTACAAACAA TAACAATGAC AGTGCTGTGT GGCTTGGAGG GTCTATGGGG
    TCCATTGCCC AGAAAAGTGA CTTATCCTCT TCATCGGGGT CCCTAACACT GTCTCATGGA
    TCCAACTCCT GTGACGCAGG AAAGGAAGAC TGCACTCTGT CCGATCTTTT GGGTGATCCA
    AGCATGCCAC TGCCAAATAG CAAACAACAA AAAGCCAAAA GACTCAAAGC AGACTCTGTC
    CTAGTGATCT CTGGTGGCCA GGGCCACAGA AAGTTAAACA AAAAGGTCAA ACCACAGCGA
    CAAGAAGAGC TGGTATCTTC TGCAATGGTT TGGCAGATCC CACTTAACAT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58472
    Clone ID Related Accession (Same CDS sequence) XM_018094424.2 , XM_012963478.3 , XM_012963476.3 , XM_002935157.5 , XM_031902163.1
    Accession Version XM_018094424.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGCAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCG AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949913.2
    CDS521..4651
    Translation

    Target ORF information:

    RefSeq Version XM_018094424.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094424.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGCAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCG AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58472
    Clone ID Related Accession (Same CDS sequence) XM_018094424.2 , XM_012963478.3 , XM_012963476.3 , XM_002935157.5 , XM_031902163.1
    Accession Version XM_012963478.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963478.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGCAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCG AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818932.2
    CDS62..4192
    Translation

    Target ORF information:

    RefSeq Version XM_012963478.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963478.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGCAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCG AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58472
    Clone ID Related Accession (Same CDS sequence) XM_018094424.2 , XM_012963478.3 , XM_012963476.3 , XM_002935157.5 , XM_031902163.1
    Accession Version XM_012963476.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963476.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGCAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCG AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818930.2
    CDS466..4596
    Translation

    Target ORF information:

    RefSeq Version XM_012963476.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963476.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGCAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCG AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58472
    Clone ID Related Accession (Same CDS sequence) XM_018094424.2 , XM_012963478.3 , XM_012963476.3 , XM_002935157.5 , XM_031902163.1
    Accession Version XM_002935157.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935157.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGCAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCG AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002935203.3
    CDS511..4641
    Translation

    Target ORF information:

    RefSeq Version XM_002935157.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002935157.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGCAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCG AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58472
    Clone ID Related Accession (Same CDS sequence) XM_018094424.2 , XM_012963478.3 , XM_012963476.3 , XM_002935157.5 , XM_031902163.1
    Accession Version XM_031902163.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGCAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCG AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031758023.1
    CDS59..4189
    Translation

    Target ORF information:

    RefSeq Version XM_031902163.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031902163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGCAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCG AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58473
    Clone ID Related Accession (Same CDS sequence) XM_012963479.3
    Accession Version XM_012963479.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963479.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGCAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ATGCCAAGAA TCCAGTCCTG ACCGAATAAC TCTCTATGGT
    CTGATGATGA AACCTATTCA GCGCTTCCCA CAGTTTATAC TTCTGCTTCA GGACATGCTA
    AAAAATACAA ACAAGGGGCA CCCAGACCGG CTCCCTCTAC AGATGGCTCT AACAGAGCTG
    GAAACCTTAG CGGACAAACT GAACGAAAGG AAACGAGATG CAGATCAGCG CTGTGAAATT
    AAGCAGATTG CAAAGGCAAT GAATGAAAGA TATCTGAATA AGCTCCTAAG TAATGGTAGC
    AGATACCTTG TTCGATCAGA TGATATGGTT GAAACCGTTT TCAATGAAAG AGGGGAGATA
    ATTAAAACAA AGCAGCGCCG GCTTTTCATG TTAAATGATG TTTTGATGTG TGCCACGGCA
    AATGCTAGGT CTTCTGTGGA AGGCAGTAGC GCAGTAACCA CAAACCAAAA GTACCTACTA
    AAATGGAGTG TACCACTAAG CCATGCAGAG GTAATTGAAT ATGGAGAAAG TGAGGACCAA
    GGAGACCAAA CTCTGTATTC TTCTTTGCAT GTTGCTGAAA AGGTTGTTGT TAGCAGTAAA
    CCAAATAAAC TGTACATGGG GCCAGGGCAG CTGTATAATG ACTTGCAGAA CCTACTTCAT
    GATTTGGATG TAGTCAACCA GATATCTCAG CTTGTTGGAC AGCTGAAGGG AAACTATCAG
    AACCTCAACC ATGCAGTTGC CCAAGACTGG TCTGTAGGGT TACAAAAGTT GATCTTGAAG
    AAAGAAAATG AAATCCGAGC TGCAGACCGC TGCAGAATTC AGCTCCAACT CCCTGGCAAA
    CAGGACAAGT CTGGACGCCC CACTTACTTT ACTGCAGTAT TTAACACATT CTCTCCTGCT
    GCCAAACAGT CATGGCTCAG TAACTTACAG ATGGCTAAGC TTGCTCTTGG AGAGGAGAAC
    AACACAGGAT GGTTCTGTCT AGAAGATGAA GGCCATCAAG TTAAAAAGGA GAAGCATCCC
    TTGCTTGTTA AACACTTACC TGTTGTCATG TCAAAGCAGC AAGAATTTAA GATTGAATGT
    GCAGTCTATA ATCCTGAACC ATATCTCAGT AGTGAAAATG ATCCAGATAC ACTTTCCATG
    CATCATGGCT TTCTATGGAT TGGCAGCTGC ACTAATCAGA TGGGGCAGAT TTCAGTTCTT
    TCCCTCCATA ATCGTAGCCC TAAGGTGTTG GAGTGTTTCA ATGTCGAATC ACGCATACTC
    TGCATGGTCT ATATCCCAGA GGAAGCAAAA CAGAAACAAC TAAGCGATGC CCCAGATTTG
    GAAAATGGGT CTTCCCGAGT ACCAGATGTG CCCACTATTT GTCTGGGCAT GGAAGAAGGA
    AGCATATGTG TCTACAAAAG TAGTCAAGGA TCTAAAAAAG TACGGTTGCA GCACTTTTTC
    ACACCAGAAA AATCAACTGT TATGAGCCTT GCTAGCATCT CTCAGTGCTT GTATGCAGGT
    CTTGTAAATG GAAATCTCGC CACCTACACA AAAGCTGAAG ATGGAAACTG GAACACAGAC
    CAACCAAAGC TAATCAAATT AGGAGTTTTG CCGGTTCGAA GTCTGCTGAT AATGGAGAGA
    ACAATCTGGG CAGCTTCTGG AGAGCAGATA TTTATTATTG GCCAGGATTC TAATGCCATT
    GAGCACCAGT TTGAGGCCCA TAGTGAAGAA GGCATGGTGA TTTCTCACAT GGCAGTAGCG
    GGTGTTGGAA TTTGGATTGC ATTCACTACT GGATCAACTC TGCGGCTCTT TCACACCGAG
    ACATTTGAAC ATTTGCAAGA TATAAATATA GCAACTCCAA TACACAACAT GTTACCAGGA
    CAGCATCGGG TGTCTGTAAC TAGTTTGCTG GTTTGTCACG GTTTGCTAAT GGTTGGCACA
    AATCTGGGAG TTATAGTTTC ATTGCCAGTT CCTCGTTTAC AAGGAATCCC AAAAGTGACC
    GGAAGAGGCA TGGTCTCGTA TCACGCTCAT CACAGTCCAG TTAAATTCCT GGTCAAGGCA
    ACTGCCATTA ATAAGAAATA CAGACGCACA CCAAGCAGAT GTAGCCAGAT TTCTCACTCG
    GAGTTAAAGA ATGAAGACTC ACACTGTACT GGTGTTCAGC AAAACGATGA AGCCGCTGCA
    TCAGCTACAA ACAATAACAA TGACAGTGCT GTGTGGCTTG GAGGGTCTAT GGGGTCCATT
    GCCCAGAAAA GTGACTTATC CTCTTCATCG GGGTCCCTAA CACTGTCTCA TGGATCCAAC
    TCCTGTGACG CAGGAAAGGA AGACTGCACT CTGTCCGATC TTTTGGGTGA TCCAAGCATG
    CCACTGCCAA ATAGCAAACA ACAAAAAGCC AAAAGACTCA AAGCAGACTC TGTCCTAGTG
    ATCTCTGGTG GCCAGGGCCA CAGAAAGTTA AACAAAAAGG TCAAACCACA GCGACAAGAA
    GAGCTGGTAT CTTCTGCAAT GGTTTGGCAG ATCCCACTTA ACATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818933.2
    CDS468..4595
    Translation

    Target ORF information:

    RefSeq Version XM_012963479.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963479.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGCAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ATGCCAAGAA TCCAGTCCTG ACCGAATAAC TCTCTATGGT
    CTGATGATGA AACCTATTCA GCGCTTCCCA CAGTTTATAC TTCTGCTTCA GGACATGCTA
    AAAAATACAA ACAAGGGGCA CCCAGACCGG CTCCCTCTAC AGATGGCTCT AACAGAGCTG
    GAAACCTTAG CGGACAAACT GAACGAAAGG AAACGAGATG CAGATCAGCG CTGTGAAATT
    AAGCAGATTG CAAAGGCAAT GAATGAAAGA TATCTGAATA AGCTCCTAAG TAATGGTAGC
    AGATACCTTG TTCGATCAGA TGATATGGTT GAAACCGTTT TCAATGAAAG AGGGGAGATA
    ATTAAAACAA AGCAGCGCCG GCTTTTCATG TTAAATGATG TTTTGATGTG TGCCACGGCA
    AATGCTAGGT CTTCTGTGGA AGGCAGTAGC GCAGTAACCA CAAACCAAAA GTACCTACTA
    AAATGGAGTG TACCACTAAG CCATGCAGAG GTAATTGAAT ATGGAGAAAG TGAGGACCAA
    GGAGACCAAA CTCTGTATTC TTCTTTGCAT GTTGCTGAAA AGGTTGTTGT TAGCAGTAAA
    CCAAATAAAC TGTACATGGG GCCAGGGCAG CTGTATAATG ACTTGCAGAA CCTACTTCAT
    GATTTGGATG TAGTCAACCA GATATCTCAG CTTGTTGGAC AGCTGAAGGG AAACTATCAG
    AACCTCAACC ATGCAGTTGC CCAAGACTGG TCTGTAGGGT TACAAAAGTT GATCTTGAAG
    AAAGAAAATG AAATCCGAGC TGCAGACCGC TGCAGAATTC AGCTCCAACT CCCTGGCAAA
    CAGGACAAGT CTGGACGCCC CACTTACTTT ACTGCAGTAT TTAACACATT CTCTCCTGCT
    GCCAAACAGT CATGGCTCAG TAACTTACAG ATGGCTAAGC TTGCTCTTGG AGAGGAGAAC
    AACACAGGAT GGTTCTGTCT AGAAGATGAA GGCCATCAAG TTAAAAAGGA GAAGCATCCC
    TTGCTTGTTA AACACTTACC TGTTGTCATG TCAAAGCAGC AAGAATTTAA GATTGAATGT
    GCAGTCTATA ATCCTGAACC ATATCTCAGT AGTGAAAATG ATCCAGATAC ACTTTCCATG
    CATCATGGCT TTCTATGGAT TGGCAGCTGC ACTAATCAGA TGGGGCAGAT TTCAGTTCTT
    TCCCTCCATA ATCGTAGCCC TAAGGTGTTG GAGTGTTTCA ATGTCGAATC ACGCATACTC
    TGCATGGTCT ATATCCCAGA GGAAGCAAAA CAGAAACAAC TAAGCGATGC CCCAGATTTG
    GAAAATGGGT CTTCCCGAGT ACCAGATGTG CCCACTATTT GTCTGGGCAT GGAAGAAGGA
    AGCATATGTG TCTACAAAAG TAGTCAAGGA TCTAAAAAAG TACGGTTGCA GCACTTTTTC
    ACACCAGAAA AATCAACTGT TATGAGCCTT GCTAGCATCT CTCAGTGCTT GTATGCAGGT
    CTTGTAAATG GAAATCTCGC CACCTACACA AAAGCTGAAG ATGGAAACTG GAACACAGAC
    CAACCAAAGC TAATCAAATT AGGAGTTTTG CCGGTTCGAA GTCTGCTGAT AATGGAGAGA
    ACAATCTGGG CAGCTTCTGG AGAGCAGATA TTTATTATTG GCCAGGATTC TAATGCCATT
    GAGCACCAGT TTGAGGCCCA TAGTGAAGAA GGCATGGTGA TTTCTCACAT GGCAGTAGCG
    GGTGTTGGAA TTTGGATTGC ATTCACTACT GGATCAACTC TGCGGCTCTT TCACACCGAG
    ACATTTGAAC ATTTGCAAGA TATAAATATA GCAACTCCAA TACACAACAT GTTACCAGGA
    CAGCATCGGG TGTCTGTAAC TAGTTTGCTG GTTTGTCACG GTTTGCTAAT GGTTGGCACA
    AATCTGGGAG TTATAGTTTC ATTGCCAGTT CCTCGTTTAC AAGGAATCCC AAAAGTGACC
    GGAAGAGGCA TGGTCTCGTA TCACGCTCAT CACAGTCCAG TTAAATTCCT GGTCAAGGCA
    ACTGCCATTA ATAAGAAATA CAGACGCACA CCAAGCAGAT GTAGCCAGAT TTCTCACTCG
    GAGTTAAAGA ATGAAGACTC ACACTGTACT GGTGTTCAGC AAAACGATGA AGCCGCTGCA
    TCAGCTACAA ACAATAACAA TGACAGTGCT GTGTGGCTTG GAGGGTCTAT GGGGTCCATT
    GCCCAGAAAA GTGACTTATC CTCTTCATCG GGGTCCCTAA CACTGTCTCA TGGATCCAAC
    TCCTGTGACG CAGGAAAGGA AGACTGCACT CTGTCCGATC TTTTGGGTGA TCCAAGCATG
    CCACTGCCAA ATAGCAAACA ACAAAAAGCC AAAAGACTCA AAGCAGACTC TGTCCTAGTG
    ATCTCTGGTG GCCAGGGCCA CAGAAAGTTA AACAAAAAGG TCAAACCACA GCGACAAGAA
    GAGCTGGTAT CTTCTGCAAT GGTTTGGCAG ATCCCACTTA ACATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10351
    Clone ID Related Accession (Same CDS sequence) XM_002935157.4 , XM_018094424.1 , XM_012963476.2 , XM_012963478.2 , XM_018094425.1
    Accession Version XM_002935157.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935157.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCA AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002935203.2
    CDS557..4687
    Translation

    Target ORF information:

    RefSeq Version XM_002935157.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002935157.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCA AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10351
    Clone ID Related Accession (Same CDS sequence) XM_002935157.4 , XM_018094424.1 , XM_012963476.2 , XM_012963478.2 , XM_018094425.1
    Accession Version XM_018094424.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCA AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949913.1
    CDS567..4697
    Translation

    Target ORF information:

    RefSeq Version XM_018094424.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094424.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCA AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10351
    Clone ID Related Accession (Same CDS sequence) XM_002935157.4 , XM_018094424.1 , XM_012963476.2 , XM_012963478.2 , XM_018094425.1
    Accession Version XM_012963476.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCA AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818930.1
    CDS513..4643
    Translation

    Target ORF information:

    RefSeq Version XM_012963476.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963476.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCA AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10351
    Clone ID Related Accession (Same CDS sequence) XM_002935157.4 , XM_018094424.1 , XM_012963476.2 , XM_012963478.2 , XM_018094425.1
    Accession Version XM_012963478.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCA AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818932.1
    CDS93..4223
    Translation

    Target ORF information:

    RefSeq Version XM_012963478.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963478.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCA AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa10351
    Clone ID Related Accession (Same CDS sequence) XM_002935157.4 , XM_018094424.1 , XM_012963476.2 , XM_012963478.2 , XM_018094425.1
    Accession Version XM_018094425.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCA AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017949914.1
    CDS60..4190
    Translation

    Target ORF information:

    RefSeq Version XM_018094425.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094425.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ACAGTGCCAA GAATCCAGTC CTGACCGAAT AACTCTCTAT
    GGTCTGATGA TGAAACCTAT TCAGCGCTTC CCACAGTTTA TACTTCTGCT TCAGGACATG
    CTAAAAAATA CAAACAAGGG GCACCCAGAC CGGCTCCCTC TACAGATGGC TCTAACAGAG
    CTGGAAACCT TAGCGGACAA ACTGAACGAA AGGAAACGAG ATGCAGATCA GCGCTGTGAA
    ATTAAGCAGA TTGCAAAGGC AATGAATGAA AGATATCTGA ATAAGCTCCT AAGTAATGGT
    AGCAGATACC TTGTTCGATC AGATGATATG GTTGAAACCG TTTTCAATGA AAGAGGGGAG
    ATAATTAAAA CAAAGCAGCG CCGGCTTTTC ATGTTAAATG ATGTTTTGAT GTGTGCCACG
    GCAAATGCTA GGTCTTCTGT GGAAGGCAGT AGCGCAGTAA CCACAAACCA AAAGTACCTA
    CTAAAATGGA GTGTACCACT AAGCCATGCA GAGGTAATTG AATATGGAGA AAGTGAGGAC
    CAAGGAGACC AAACTCTGTA TTCTTCTTTG CATGTTGCTG AAAAGGTTGT TGTTAGCAGT
    AAACCAAATA AACTGTACAT GGGGCCAGGG CAGCTGTATA ATGACTTGCA GAACCTACTT
    CATGATTTGG ATGTAGTCAA CCAGATATCT CAGCTTGTTG GACAGCTGAA GGGAAACTAT
    CAGAACCTCA ACCATGCAGT TGCCCAAGAC TGGTCTGTAG GGTTACAAAA GTTGATCTTG
    AAGAAAGAAA ATGAAATCCG AGCTGCAGAC CGCTGCAGAA TTCAGCTCCA ACTCCCTGGC
    AAACAGGACA AGTCTGGACG CCCCACTTAC TTTACTGCAG TATTTAACAC ATTCTCTCCT
    GCTGCCAAAC AGTCATGGCT CAGTAACTTA CAGATGGCTA AGCTTGCTCT TGGAGAGGAG
    AACAACACAG GATGGTTCTG TCTAGAAGAT GAAGGCCATC AAGTTAAAAA GGAGAAGCAT
    CCCTTGCTTG TTAAACACTT ACCTGTTGTC ATGTCAAAGC AGCAAGAATT TAAGATTGAA
    TGTGCAGTCT ATAATCCTGA ACCATATCTC AGTAGTGAAA ATGATCCAGA TACACTTTCC
    ATGCATCATG GCTTTCTATG GATTGGCAGC TGCACTAATC AGATGGGGCA GATTTCAGTT
    CTTTCCCTCC ATAATCGTAG CCCTAAGGTG TTGGAGTGTT TCAATGTCGA ATCACGCATA
    CTCTGCATGG TCTATATCCC AGAGGAAGCA AAACAGAAAC AACTAAGCGA TGCCCCAGAT
    TTGGAAAATG GGTCTTCCCA AGTACCAGAT GTGCCCACTA TTTGTCTGGG CATGGAAGAA
    GGAAGCATAT GTGTCTACAA AAGTAGTCAA GGATCTAAAA AAGTACGGTT GCAGCACTTT
    TTCACACCAG AAAAATCAAC TGTTATGAGC CTTGCTAGCA TCTCTCAGTG CTTGTATGCA
    GGTCTTGTAA ATGGAAATCT CGCCACCTAC ACAAAAGCTG AAGATGGAAA CTGGAACACA
    GACCAACCAA AGCTAATCAA ATTAGGAGTT TTGCCGGTTC GAAGTCTGCT GATAATGGAG
    AGAACAATCT GGGCAGCTTC TGGAGAGCAG ATATTTATTA TTGGCCAGGA TTCTAATGCC
    ATTGAGCACC AGTTTGAGGC CCATAGTGAA GAAGGCATGG TGATTTCTCA CATGGCAGTA
    GCGGGTGTTG GAATTTGGAT TGCATTCACT ACTGGATCAA CTCTGCGGCT CTTTCACACC
    GAGACATTTG AACATTTGCA AGATATAAAT ATAGCAACTC CAATACACAA CATGTTACCA
    GGACAGCATC GGGTGTCTGT AACTAGTTTG CTGGTTTGTC ACGGTTTGCT AATGGTTGGC
    ACAAATCTGG GAGTTATAGT TTCATTGCCA GTTCCTCGTT TACAAGGAAT CCCAAAAGTG
    ACCGGAAGAG GCATGGTCTC GTATCACGCT CATCACAGTC CAGTTAAATT CCTGGTCAAG
    GCAACTGCCA TTAATAAGAA ATACAGACGC ACACCAAGCA GATGTAGCCA GATTTCTCAC
    TCGGAGTTAA AGAATGAAGA CTCACACTGT ACTGGTGTTC AGCAAAACGA TGAAGCCGCT
    GCATCAGCTA CAAACAATAA CAATGACAGT GCTGTGTGGC TTGGAGGGTC TATGGGGTCC
    ATTGCCCAGA AAAGTGACTT ATCCTCTTCA TCGGGGTCCC TAACACTGTC TCATGGATCC
    AACTCCTGTG ACGCAGGAAA GGAAGACTGC ACTCTGTCCG ATCTTTTGGG TGATCCAAGC
    ATGCCACTGC CAAATAGCAA ACAACAAAAA GCCAAAAGAC TCAAAGCAGA CTCTGTCCTA
    GTGATCTCTG GTGGCCAGGG CCACAGAAAG TTAAACAAAA AGGTCAAACC ACAGCGACAA
    GAAGAGCTGG TATCTTCTGC AATGGTTTGG CAGATCCCAC TTAACATGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa34836
    Clone ID Related Accession (Same CDS sequence) XM_012963479.2
    Accession Version XM_012963479.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product rho guanine nucleotide exchange factor 10 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ATGCCAAGAA TCCAGTCCTG ACCGAATAAC TCTCTATGGT
    CTGATGATGA AACCTATTCA GCGCTTCCCA CAGTTTATAC TTCTGCTTCA GGACATGCTA
    AAAAATACAA ACAAGGGGCA CCCAGACCGG CTCCCTCTAC AGATGGCTCT AACAGAGCTG
    GAAACCTTAG CGGACAAACT GAACGAAAGG AAACGAGATG CAGATCAGCG CTGTGAAATT
    AAGCAGATTG CAAAGGCAAT GAATGAAAGA TATCTGAATA AGCTCCTAAG TAATGGTAGC
    AGATACCTTG TTCGATCAGA TGATATGGTT GAAACCGTTT TCAATGAAAG AGGGGAGATA
    ATTAAAACAA AGCAGCGCCG GCTTTTCATG TTAAATGATG TTTTGATGTG TGCCACGGCA
    AATGCTAGGT CTTCTGTGGA AGGCAGTAGC GCAGTAACCA CAAACCAAAA GTACCTACTA
    AAATGGAGTG TACCACTAAG CCATGCAGAG GTAATTGAAT ATGGAGAAAG TGAGGACCAA
    GGAGACCAAA CTCTGTATTC TTCTTTGCAT GTTGCTGAAA AGGTTGTTGT TAGCAGTAAA
    CCAAATAAAC TGTACATGGG GCCAGGGCAG CTGTATAATG ACTTGCAGAA CCTACTTCAT
    GATTTGGATG TAGTCAACCA GATATCTCAG CTTGTTGGAC AGCTGAAGGG AAACTATCAG
    AACCTCAACC ATGCAGTTGC CCAAGACTGG TCTGTAGGGT TACAAAAGTT GATCTTGAAG
    AAAGAAAATG AAATCCGAGC TGCAGACCGC TGCAGAATTC AGCTCCAACT CCCTGGCAAA
    CAGGACAAGT CTGGACGCCC CACTTACTTT ACTGCAGTAT TTAACACATT CTCTCCTGCT
    GCCAAACAGT CATGGCTCAG TAACTTACAG ATGGCTAAGC TTGCTCTTGG AGAGGAGAAC
    AACACAGGAT GGTTCTGTCT AGAAGATGAA GGCCATCAAG TTAAAAAGGA GAAGCATCCC
    TTGCTTGTTA AACACTTACC TGTTGTCATG TCAAAGCAGC AAGAATTTAA GATTGAATGT
    GCAGTCTATA ATCCTGAACC ATATCTCAGT AGTGAAAATG ATCCAGATAC ACTTTCCATG
    CATCATGGCT TTCTATGGAT TGGCAGCTGC ACTAATCAGA TGGGGCAGAT TTCAGTTCTT
    TCCCTCCATA ATCGTAGCCC TAAGGTGTTG GAGTGTTTCA ATGTCGAATC ACGCATACTC
    TGCATGGTCT ATATCCCAGA GGAAGCAAAA CAGAAACAAC TAAGCGATGC CCCAGATTTG
    GAAAATGGGT CTTCCCAAGT ACCAGATGTG CCCACTATTT GTCTGGGCAT GGAAGAAGGA
    AGCATATGTG TCTACAAAAG TAGTCAAGGA TCTAAAAAAG TACGGTTGCA GCACTTTTTC
    ACACCAGAAA AATCAACTGT TATGAGCCTT GCTAGCATCT CTCAGTGCTT GTATGCAGGT
    CTTGTAAATG GAAATCTCGC CACCTACACA AAAGCTGAAG ATGGAAACTG GAACACAGAC
    CAACCAAAGC TAATCAAATT AGGAGTTTTG CCGGTTCGAA GTCTGCTGAT AATGGAGAGA
    ACAATCTGGG CAGCTTCTGG AGAGCAGATA TTTATTATTG GCCAGGATTC TAATGCCATT
    GAGCACCAGT TTGAGGCCCA TAGTGAAGAA GGCATGGTGA TTTCTCACAT GGCAGTAGCG
    GGTGTTGGAA TTTGGATTGC ATTCACTACT GGATCAACTC TGCGGCTCTT TCACACCGAG
    ACATTTGAAC ATTTGCAAGA TATAAATATA GCAACTCCAA TACACAACAT GTTACCAGGA
    CAGCATCGGG TGTCTGTAAC TAGTTTGCTG GTTTGTCACG GTTTGCTAAT GGTTGGCACA
    AATCTGGGAG TTATAGTTTC ATTGCCAGTT CCTCGTTTAC AAGGAATCCC AAAAGTGACC
    GGAAGAGGCA TGGTCTCGTA TCACGCTCAT CACAGTCCAG TTAAATTCCT GGTCAAGGCA
    ACTGCCATTA ATAAGAAATA CAGACGCACA CCAAGCAGAT GTAGCCAGAT TTCTCACTCG
    GAGTTAAAGA ATGAAGACTC ACACTGTACT GGTGTTCAGC AAAACGATGA AGCCGCTGCA
    TCAGCTACAA ACAATAACAA TGACAGTGCT GTGTGGCTTG GAGGGTCTAT GGGGTCCATT
    GCCCAGAAAA GTGACTTATC CTCTTCATCG GGGTCCCTAA CACTGTCTCA TGGATCCAAC
    TCCTGTGACG CAGGAAAGGA AGACTGCACT CTGTCCGATC TTTTGGGTGA TCCAAGCATG
    CCACTGCCAA ATAGCAAACA ACAAAAAGCC AAAAGACTCA AAGCAGACTC TGTCCTAGTG
    ATCTCTGGTG GCCAGGGCCA CAGAAAGTTA AACAAAAAGG TCAAACCACA GCGACAAGAA
    GAGCTGGTAT CTTCTGCAAT GGTTTGGCAG ATCCCACTTA ACATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012818933.1
    CDS514..4641
    Translation

    Target ORF information:

    RefSeq Version XM_012963479.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis Rho guanine nucleotide exchange factor 10 (arhgef10), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012963479.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACATTT CTGACAATGT AGATACTGAG GACTTTCCAC CCCCACCACC TGAAGTTTAT 
    GTTGACGAGC TGACACTTAA TGGCCCTGCT TATGAAAAAG ATGATGATGA AGATGAAGGA
    GAAAAATTTG ATTTTGATAG TGGGGATGAA ATTCCAGAAG CAGATCGACA GCCATTGGAA
    GTTCCTGGTG ATACTCTCAT CGACCAGGGA TTTCAATTTC CAGACCCTCC AGTGCTGCCC
    AATGAAATTA CAAAACAGGA ATTGCCAACA GAAACTAGTA CGAAGATGGC AACAAAGAAG
    GTGAACCCAT ATTCTATTAT TGACATAGCG CCATTTCAAG AGGTTGATTC AGCTACTTCA
    GAAGATCTTG TATCTACTGA CGAAAGCACC AGTCCACGAG TGCCAAGTGG ATATTCTGTT
    CCTGTGCCAT GTGGATATGC AGTCCCTTCC AACTTACCCT TGTTGCAGCC AGCATACTCC
    ACACCAGTTA TAATTCGCAA CATATCTATT GATGAGCAAG GAGTGCAAGA GGTTGTTCCA
    AATCCACATT TTCATTTCAC TAGTGAAGAT GATCACCTTT CCAGGCCTAT AGGCAGAGAC
    GGAGAACTCT CGAGTGAAGA TTTCCAGTCG ACCAGAGAGG AAGATGTGTT GGCAAAGTGG
    GTTGCAAATC CTGCAAATAC AGAATGGATG GAGAATCCTG ATGAAGTCAT TTATGATGAT
    GTACCAAGGG AGAACTCAGA CTCAGCAACA GACCCTGAAG AAATGATATA CGATGATGTG
    GAAAATGGTG AAGATGGTGG AAACAGCTCA TTGGACTATG GCTGGAGCTC TAGTGAATTT
    GAAAGCTATG AAGAACACAG TGACTCGGAG TCAAAACATG GGGTACCAAA CTCATTTTTG
    AGAGGAAACA ACAAAAGGCA TCTTTCTCAG GATCTTACAC GTTTAAAGCA GCATTATGAG
    AAGAAGATGA AAGATCTGGT GGCAAATAGA GTGGGAGCAG TAGAGATCCA ACATTTTAAG
    CACAAACATG AACTTAAGAT GCAAAGGCTG GTAAAGGCTG CTAAAGAGGG GACAAAAGAT
    GGACTTGAAA AAACCAAGGC TGCTGTGAAG AAAGGTCGTT CCTACATAAG GACCAAATCA
    TTTGTCTTGC ATGATCGCAA ATCCACCAGT CTTGAAGATG ACGAGTTGAA TTTATTTATT
    GATGTGGACT GCATGCACAT AGAGGCGATC ATGTCTCCAA TGCCAGAAGG ACTATCACAA
    CAGCAGGTTG TCAGACGCTA CATTCTAGGA TCAATTGTGG AAAGTGAGAA GAACTATGTA
    GACTCATTAA AAAGAATTCT CGAGCAATAT GAGAAGCCAT TGTGGGAGAC AGAGCCAAGG
    TTATTAAGTG AAAGAAAGCT GAAAATAGTC TTCTATCGCA TAAAGGAAAT TCTCCAGTGT
    CACTCACTGT TCCAGATTGC TCTCGCCAGC CGAGTGGCAG AGTGGGATAC TGCCGAAATG
    ATTGGGGATG TGTTTGTAGC TTCATTTTCA AAATCCATGG TCCTGGATGC ATACAGTGAG
    TATGTGAATA ATTTCAGTAC AGCCGTCGGA GTTCTCAGGA AAGCCTGTGC CACAAAACCA
    TCTTTTTTGG AGTTTTTAAA ATGCCAAGAA TCCAGTCCTG ACCGAATAAC TCTCTATGGT
    CTGATGATGA AACCTATTCA GCGCTTCCCA CAGTTTATAC TTCTGCTTCA GGACATGCTA
    AAAAATACAA ACAAGGGGCA CCCAGACCGG CTCCCTCTAC AGATGGCTCT AACAGAGCTG
    GAAACCTTAG CGGACAAACT GAACGAAAGG AAACGAGATG CAGATCAGCG CTGTGAAATT
    AAGCAGATTG CAAAGGCAAT GAATGAAAGA TATCTGAATA AGCTCCTAAG TAATGGTAGC
    AGATACCTTG TTCGATCAGA TGATATGGTT GAAACCGTTT TCAATGAAAG AGGGGAGATA
    ATTAAAACAA AGCAGCGCCG GCTTTTCATG TTAAATGATG TTTTGATGTG TGCCACGGCA
    AATGCTAGGT CTTCTGTGGA AGGCAGTAGC GCAGTAACCA CAAACCAAAA GTACCTACTA
    AAATGGAGTG TACCACTAAG CCATGCAGAG GTAATTGAAT ATGGAGAAAG TGAGGACCAA
    GGAGACCAAA CTCTGTATTC TTCTTTGCAT GTTGCTGAAA AGGTTGTTGT TAGCAGTAAA
    CCAAATAAAC TGTACATGGG GCCAGGGCAG CTGTATAATG ACTTGCAGAA CCTACTTCAT
    GATTTGGATG TAGTCAACCA GATATCTCAG CTTGTTGGAC AGCTGAAGGG AAACTATCAG
    AACCTCAACC ATGCAGTTGC CCAAGACTGG TCTGTAGGGT TACAAAAGTT GATCTTGAAG
    AAAGAAAATG AAATCCGAGC TGCAGACCGC TGCAGAATTC AGCTCCAACT CCCTGGCAAA
    CAGGACAAGT CTGGACGCCC CACTTACTTT ACTGCAGTAT TTAACACATT CTCTCCTGCT
    GCCAAACAGT CATGGCTCAG TAACTTACAG ATGGCTAAGC TTGCTCTTGG AGAGGAGAAC
    AACACAGGAT GGTTCTGTCT AGAAGATGAA GGCCATCAAG TTAAAAAGGA GAAGCATCCC
    TTGCTTGTTA AACACTTACC TGTTGTCATG TCAAAGCAGC AAGAATTTAA GATTGAATGT
    GCAGTCTATA ATCCTGAACC ATATCTCAGT AGTGAAAATG ATCCAGATAC ACTTTCCATG
    CATCATGGCT TTCTATGGAT TGGCAGCTGC ACTAATCAGA TGGGGCAGAT TTCAGTTCTT
    TCCCTCCATA ATCGTAGCCC TAAGGTGTTG GAGTGTTTCA ATGTCGAATC ACGCATACTC
    TGCATGGTCT ATATCCCAGA GGAAGCAAAA CAGAAACAAC TAAGCGATGC CCCAGATTTG
    GAAAATGGGT CTTCCCAAGT ACCAGATGTG CCCACTATTT GTCTGGGCAT GGAAGAAGGA
    AGCATATGTG TCTACAAAAG TAGTCAAGGA TCTAAAAAAG TACGGTTGCA GCACTTTTTC
    ACACCAGAAA AATCAACTGT TATGAGCCTT GCTAGCATCT CTCAGTGCTT GTATGCAGGT
    CTTGTAAATG GAAATCTCGC CACCTACACA AAAGCTGAAG ATGGAAACTG GAACACAGAC
    CAACCAAAGC TAATCAAATT AGGAGTTTTG CCGGTTCGAA GTCTGCTGAT AATGGAGAGA
    ACAATCTGGG CAGCTTCTGG AGAGCAGATA TTTATTATTG GCCAGGATTC TAATGCCATT
    GAGCACCAGT TTGAGGCCCA TAGTGAAGAA GGCATGGTGA TTTCTCACAT GGCAGTAGCG
    GGTGTTGGAA TTTGGATTGC ATTCACTACT GGATCAACTC TGCGGCTCTT TCACACCGAG
    ACATTTGAAC ATTTGCAAGA TATAAATATA GCAACTCCAA TACACAACAT GTTACCAGGA
    CAGCATCGGG TGTCTGTAAC TAGTTTGCTG GTTTGTCACG GTTTGCTAAT GGTTGGCACA
    AATCTGGGAG TTATAGTTTC ATTGCCAGTT CCTCGTTTAC AAGGAATCCC AAAAGTGACC
    GGAAGAGGCA TGGTCTCGTA TCACGCTCAT CACAGTCCAG TTAAATTCCT GGTCAAGGCA
    ACTGCCATTA ATAAGAAATA CAGACGCACA CCAAGCAGAT GTAGCCAGAT TTCTCACTCG
    GAGTTAAAGA ATGAAGACTC ACACTGTACT GGTGTTCAGC AAAACGATGA AGCCGCTGCA
    TCAGCTACAA ACAATAACAA TGACAGTGCT GTGTGGCTTG GAGGGTCTAT GGGGTCCATT
    GCCCAGAAAA GTGACTTATC CTCTTCATCG GGGTCCCTAA CACTGTCTCA TGGATCCAAC
    TCCTGTGACG CAGGAAAGGA AGACTGCACT CTGTCCGATC TTTTGGGTGA TCCAAGCATG
    CCACTGCCAA ATAGCAAACA ACAAAAAGCC AAAAGACTCA AAGCAGACTC TGTCCTAGTG
    ATCTCTGGTG GCCAGGGCCA CAGAAAGTTA AACAAAAAGG TCAAACCACA GCGACAAGAA
    GAGCTGGTAT CTTCTGCAAT GGTTTGGCAG ATCCCACTTA ACATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.