dennd4a gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_018092497.1	XP_017947986.1	C-myc promoter-binding protein isoform X1
XM_031899251.1	XP_031755111.1	C-myc promoter-binding protein isoform X2
XM_031899254.1	XP_031755114.1	C-myc promoter-binding protein isoform X4
XM_031899250.1	XP_031755110.1	C-myc promoter-binding protein isoform X1
XM_018092501.2	XP_017947990.2	C-myc promoter-binding protein isoform X6
XM_018092498.1	XP_017947987.1	C-myc promoter-binding protein isoform X2
XM_018092499.1	XP_017947988.1	C-myc promoter-binding protein isoform X3
XM_018092501.1	XP_017947990.1	C-myc promoter-binding protein isoform X5
XM_031899252.1	XP_031755112.1	C-myc promoter-binding protein isoform X3
XM_018092500.1	XP_017947989.1	C-myc promoter-binding protein isoform X4
XM_031899253.1	XP_031755113.1	C-myc promoter-binding protein isoform X4
XM_031899255.1	XP_031755115.1	C-myc promoter-binding protein isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa43406	XM_018092497.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa43407	XM_018092498.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa43408	XM_018092499.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa43409	XM_018092500.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa43410	XM_018092501.1 Latest version!	Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OXa55245	XM_031899250.1 Latest version!	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55246	XM_031899251.1 Latest version!	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55247	XM_031899252.1 Latest version!	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55248	XM_031899254.1 Latest version!	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55248	XM_031899253.1 Latest version!	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55249	XM_031899255.1 Latest version!	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa55250	XM_018092501.2 Latest version!	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

dennd4a ( XM_018092497.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947986.1 Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa43406
Clone ID Related Accession (Same CDS sequence)	XM_018092497.1
Accession Version	XM_018092497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCTGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGCTTT TCTCAGAGAG GAATAATGCA 
GATAAAGGTG GCAAGGCGGA TGTGGATCGA CCAGGAGAGA CACGTCTTAT TGAACTGGAT 
GAGTCTCACA GAAGTGAGCA TACAGTGTTC ATCACACCAC CAGAGATTCC CCACTTACCA 
AATGGCGAGG AGCCCCCTAA GAACTATAGC TACAATGGGT TCCCTGTCCT TATCTTGGAT 
CTCTTTGACA CGCCAGATGG ACTCATGAAA AGTCACAAAC TTAGTACAAA AGTCACCACA 
CCCTGCAGTC CTTCTCCAAT GTTTAGAAGA ACAAAACAGG AAATCCGGTC AGCACATAAA 
ATAGCAAAGC AGTACTCCTC CATCCCCCAG ATGTGGTCCA GGTGTCTACT GAGACATTGC 
TATGGACTTT GGTTTATCTG TCTGCCCGCA TATGTGAAAT TCTGCCATTC CAAAGTGAGA 
GCACTGCGCA CAGCCTACGA TGTGTTGCGA AAAATGCACA TGAAAAAGAT TGACTCTCCT 
GATGAGGTTT GCTATAGGGT TCTAATGCAG TTGTGTGGCC AATATGGGCA GCCTGTCCTT 
GCTGTTAAAG TTCTCTTTGA AATGAGAAAG GTCGGCGTGC AGCCAAATGC TATAACATAT 
GGCTATTATA ATAAGGCTGT CCTGGAAAGC GTATGGCCCT CTGGGAATAG AGGGGGTTAC 
TTTCTGTGGA TAAAGGTGAG GAATGTGGTG CTAGCAGTAG CCCAGTTCAA ACAGGCTTTG 
ATAAAGCAAC ACGCTTCAGC TCATCAAACT CCTGCTTCAG ATTGCAGTGA TTTGGACGCA 
GTCAGCCATG GCAGTTTGGA TAGTTCTGTT GACATGAACC CTACAGAACA GCCTTCATTC 
AGTACAGATG TAACATGTGC TGATCCAGCT GACGACCGAT CTAGTACAGG AGGCCAGTCA 
GACCTTGGCT ACAGTTCATT ATCAAAAGAA GAAGTAAGAA GAGGAGCCGT GCATGACATA 
CAGGAGGAGA AGGAAGACTC CAGAGATAGT GACTCCAGTT CGTCATCTGA TTCAGAAAGT 
TCCAAGAGCA GCAGAGACTG TGTGCCACAA GGAACTGGCA GTCAGGGTCA ACAAACATTG 
GCAAGCACAT CTAGTATAGT CCGGAAAGAA GCAAAATCAG ACAGTGCAAG CAGTGACAAC 
TCAGCAGGTC TACTGTTCAC ACCATCCTTT AGTGAGAATT GTGAAAGTGA AGTGTATCAG 
TGCTGGAGTC TACATAAAAG ACACAAGAGT CCTGTGGAGT CAGATCTGAA AAAACAGATG 
GAATGGGAAA AGAGGAACAG GCATCTAAGC AGTGGCAATG TTGAGGAATT ATTTATCAGT 
AAGTCTGATG GACAGAATAA CACTGAAAAC ATGGTGGACA AACTTGGAGC AGATGCAAAA 
ATACTTAGCA CTGTATATAA GCAACACATG GCACGGCCCA AGACCCTGGA CTTAAATAAA 
ATGAATCCCA GAAAGTCAAA GGAGGTTGTT GAAAAAGAAT CTAGTGATGA TGAGTCTCCC 
ATAGGAGGAG CAGATGACAA AGTGGACTCC CCTGTTATTT TTGACCTTGA AGATCTAGAT 
AATGATTCTG ATGAACTTAA ATCAGCAAAA CCATGTGTCC GGAAGGCCAA ACTTGCTCAA 
AGAAGTATCA GCATGCCCTT TTCATCTTCC ATGTTTCTGG CTAAGCCAGC TGAAAAAACA 
GACGTGAAGG CTGGATTAGA CCCTTTGTCA TTGCTGGCAG CTGAAACAGA ACAGGCAGAA 
GTAGAACAGG AGGAGGAAGA TGACAAAAGC TTACTAACAC CATCTGCTAA AAGGGACCTT 
GCAGAGGAAA TAGAAATGTA CATGAACAAT ATAAGCAGCC CCCTTGCAAG TCGAACCCCT 
AGCATGGACC TTGACAGATC CAGTTCTGAA CTAAATAAAA CATGTAAAGC TGTAGCACTC 
AACAGGCGCT TGAGTGTTCC TGCTAGATCC CCACAAGAAC CAAGACTAAC CAGATCAATT 
ACTGCCCACA GTAAACGCGA CAAAAGACAA AGAAGCAGGC TCTGGTCTTC TCCTGCTGTT 
TCCGAGAACA ATCCTCAGAA TGAGCAAAGC CCAGAATCTG CCCTTGCTTT AATGTCCCCA 
ACATCATTTA ACATAGATAC ACTTCTCACG CCAACCTTTG ATGTTCTTAG GAGCAACATG 
TTCTCAGCTG GCAAAGGAGT AGCCAAGAAG GCCAGCAAAT GGTATTCAAA GATCGCAAAT 
TATGCGGGGC CTGCAAAGGA TCAGAATTCT GACCGAGCCA GTCTGTCTTC AGGAGGAGCA 
GGGGATACTG AATCCTACTC TTTAAATGAT GATGATTTTG ATGGCAGTGC TTCTCTGCAT 
GAGACTGATG TCTTTCCTGG AACCAATACC ATTGGTGTAA GCAGATGCAG CTTGGAGAGT 
AGCACATCTC AAGACAGTAA CTCTAAACAA CTTTACAATA ATGGTCTGCC ATCAACAGCA 
TCTCCAAACT ACAAGAGTGA GATTCTTTCA TCCTGCAATT CCAGTAGCAC TAGTGTCTTC 
CAGAACTATG CTATGGAGGT TCTGATATCT AGCTGTTCAC GTTGCTTGAC CTGTGACGGT 
CTTGTTCATG ATGAGGAGAT TATGGCAGGC TGGACGGCAG ATGACTCAAA CCTTAACACC 
ACATGTCCTT TTTGCGGGAA CTCATTTCTT CCATCTTTAA ACGTTGAAAT TAGGGACCTG 
CGACGCCCTG GAAGGTACTT TCTGAAGGCC AGCCCATCTG CAGAAAGTAT GCCGCATTCT 
TCAGTATCCT CACAAAGACC TAACTCAGAT ATTCTGACTC CGGCTTCTCC CTGTGCTTCC 
ACCAAAGTCA AGGATTTTAG TTTGTGCAGT AAAACCAAAC AAGAGAGAAC TCGGTCTGTA 
ACTGTTGAGA TCCCAGTGGG GAAGAGAACC AGAGATCTCA ATGGGTCCTT ATGCCCGAAT 
CAGCCCATTG CACGAAGTAT CAGCACATTT GGTCCCTTAG ATGAACAGCC ATCTCATGAC 
CAGAGCCATG TTATTGCCAC TGGCAGCCTC CCAACTTGCT TCCAGGCACC TCCTGAGTCA 
GGATTGGAAT GGAAGATGTC AAATCCAGAG CCAATCACAG TCCCTTACCT TAGCCCGCTG 
GTTGTCTGGA AGGAGCTGGA GAGCCTGCTA GAAAATGAGG GTGACCAATC GATTACAGTT 
TCTGGATTTG TGGACCATCA TCCGATAGTA TTCTGGAATT TGGTGTGGTA TTTCAGGAGG 
CTGGATCTCC CAAGTAACCT CCCGGGATTA ATTCTGTCTT CGGAACAATG CAATAAAAAT 
TCAAAGGTCT CCAGGAGCTG TATGTCTGCA GACAGCAAAC ATGTCCTTAT TCAAGTGCTG 
TGGGACAATC TGAAATTGCA CCAGGATCCC CGGCAGCCTC TCTACATCCT CTGGAACACT 
ATTAGTCAAA ACCGCACTGT ATTGTTTGAT ACACAGGAAC ATGCGCTTGC TGTGCCACAG 
ATCAAAGAGG AGGAATTGTT TGATCAGCAG TTTCTCAGAG GGATAGTAAG AAGCATAAAG 
AACAACAATG TTTATGGTCC TGTGAGCCAG ATATTGGACT GTATTAAACA GGTCCCTGGA 
AGAACGAGGC AGAGGAGTCT GTACAGGGAA ATTCTTTTTC TTTCTCTTGT TGCCTTGGGA 
AGAGAGAATA TCGATATTGA TGTATTTGAT CGGGAATATA AAACTGCATA CGAGCGCCTC 
ACCCCTGAGC AGCTGAACTG CACACAGATT TGTGATCGGC CTCCCAGCAT TGGAGTGATG 
GAGTGCAGGA AGACTTTCGG ACAGCCATTC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947986.1
CDS	27..5822
Translation	MMEDKGPRVADYFVVTGLTDSSKPLEEEIHFNDACHKTAKPKEPITDVTVIIKSLGEEVPQGYVCIDLTPTGLSADLNNGSIRGPQIYLCYRRGRDKPPLTDLGVLYEWKERLKQGCKIIQATPYGRPANISGSASSQRVFITYRRAAESMTQNSLAVTDICIIIPSKGETPPHTFCKVEKNLNSSVWGSAVYLCYKKSVAKTNAVSYKAGLICRYPEEDYESFPLPESVPLFCLPMGATIECWPSDSNYPLPVFSTFVLTGASAEKVYGAAIQFYEPYAQDQLTEKQQVHLGLLSSAEGQMDSSKSIHVNKCICLLSHWPFFDAFKKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDTLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQIEGKRNVTWKILPKKACKNLLNTLCNLYQQLAELQHRPREEAFMELAMNDYDFNYGKRMHQLDMEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFELQGFLKSRDRSHHKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVEKLFSERNNADKGGKADVDRPGETRLIELDESHRSEHTVFITPPEIPHLPNGEEPPKNYSYNGFPVLILDLFDTPDGLMKSHKLSTKVTTPCSPSPMFRRTKQEIRSAHKIAKQYSSIPQMWSRCLLRHCYGLWFICLPAYVKFCHSKVRALRTAYDVLRKMHMKKIDSPDEVCYRVLMQLCGQYGQPVLAVKVLFEMRKVGVQPNAITYGYYNKAVLESVWPSGNRGGYFLWIKVRNVVLAVAQFKQALIKQHASAHQTPASDCSDLDAVSHGSLDSSVDMNPTEQPSFSTDVTCADPADDRSSTGGQSDLGYSSLSKEEVRRGAVHDIQEEKEDSRDSDSSSSSDSESSKSSRDCVPQGTGSQGQQTLASTSSIVRKEAKSDSASSDNSAGLLFTPSFSENCESEVYQCWSLHKRHKSPVESDLKKQMEWEKRNRHLSSGNVEELFISKSDGQNNTENMVDKLGADAKILSTVYKQHMARPKTLDLNKMNPRKSKEVVEKESSDDESPIGGADDKVDSPVIFDLEDLDNDSDELKSAKPCVRKAKLAQRSISMPFSSSMFLAKPAEKTDVKAGLDPLSLLAAETEQAEVEQEEEDDKSLLTPSAKRDLAEEIEMYMNNISSPLASRTPSMDLDRSSSELNKTCKAVALNRRLSVPARSPQEPRLTRSITAHSKRDKRQRSRLWSSPAVSENNPQNEQSPESALALMSPTSFNIDTLLTPTFDVLRSNMFSAGKGVAKKASKWYSKIANYAGPAKDQNSDRASLSSGGAGDTESYSLNDDDFDGSASLHETDVFPGTNTIGVSRCSLESSTSQDSNSKQLYNNGLPSTASPNYKSEILSSCNSSSTSVFQNYAMEVLISSCSRCLTCDGLVHDEEIMAGWTADDSNLNTTCPFCGNSFLPSLNVEIRDLRRPGRYFLKASPSAESMPHSSVSSQRPNSDILTPASPCASTKVKDFSLCSKTKQERTRSVTVEIPVGKRTRDLNGSLCPNQPIARSISTFGPLDEQPSHDQSHVIATGSLPTCFQAPPESGLEWKMSNPEPITVPYLSPLVVWKELESLLENEGDQSITVSGFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEQCNKNSKVSRSCMSADSKHVLIQVLWDNLKLHQDPRQPLYILWNTISQNRTVLFDTQEHALAVPQIKEEELFDQQFLRGIVRSIKNNNVYGPVSQILDCIKQVPGRTRQRSLYREILFLSLVALGRENIDIDVFDREYKTAYERLTPEQLNCTQICDRPPSIGVMECRKTFGQPFL

Target ORF information:

RefSeq Version	XM_018092497.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092497.1

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCTGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGCTTT TCTCAGAGAG GAATAATGCA 
GATAAAGGTG GCAAGGCGGA TGTGGATCGA CCAGGAGAGA CACGTCTTAT TGAACTGGAT 
GAGTCTCACA GAAGTGAGCA TACAGTGTTC ATCACACCAC CAGAGATTCC CCACTTACCA 
AATGGCGAGG AGCCCCCTAA GAACTATAGC TACAATGGGT TCCCTGTCCT TATCTTGGAT 
CTCTTTGACA CGCCAGATGG ACTCATGAAA AGTCACAAAC TTAGTACAAA AGTCACCACA 
CCCTGCAGTC CTTCTCCAAT GTTTAGAAGA ACAAAACAGG AAATCCGGTC AGCACATAAA 
ATAGCAAAGC AGTACTCCTC CATCCCCCAG ATGTGGTCCA GGTGTCTACT GAGACATTGC 
TATGGACTTT GGTTTATCTG TCTGCCCGCA TATGTGAAAT TCTGCCATTC CAAAGTGAGA 
GCACTGCGCA CAGCCTACGA TGTGTTGCGA AAAATGCACA TGAAAAAGAT TGACTCTCCT 
GATGAGGTTT GCTATAGGGT TCTAATGCAG TTGTGTGGCC AATATGGGCA GCCTGTCCTT 
GCTGTTAAAG TTCTCTTTGA AATGAGAAAG GTCGGCGTGC AGCCAAATGC TATAACATAT 
GGCTATTATA ATAAGGCTGT CCTGGAAAGC GTATGGCCCT CTGGGAATAG AGGGGGTTAC 
TTTCTGTGGA TAAAGGTGAG GAATGTGGTG CTAGCAGTAG CCCAGTTCAA ACAGGCTTTG 
ATAAAGCAAC ACGCTTCAGC TCATCAAACT CCTGCTTCAG ATTGCAGTGA TTTGGACGCA 
GTCAGCCATG GCAGTTTGGA TAGTTCTGTT GACATGAACC CTACAGAACA GCCTTCATTC 
AGTACAGATG TAACATGTGC TGATCCAGCT GACGACCGAT CTAGTACAGG AGGCCAGTCA 
GACCTTGGCT ACAGTTCATT ATCAAAAGAA GAAGTAAGAA GAGGAGCCGT GCATGACATA 
CAGGAGGAGA AGGAAGACTC CAGAGATAGT GACTCCAGTT CGTCATCTGA TTCAGAAAGT 
TCCAAGAGCA GCAGAGACTG TGTGCCACAA GGAACTGGCA GTCAGGGTCA ACAAACATTG 
GCAAGCACAT CTAGTATAGT CCGGAAAGAA GCAAAATCAG ACAGTGCAAG CAGTGACAAC 
TCAGCAGGTC TACTGTTCAC ACCATCCTTT AGTGAGAATT GTGAAAGTGA AGTGTATCAG 
TGCTGGAGTC TACATAAAAG ACACAAGAGT CCTGTGGAGT CAGATCTGAA AAAACAGATG 
GAATGGGAAA AGAGGAACAG GCATCTAAGC AGTGGCAATG TTGAGGAATT ATTTATCAGT 
AAGTCTGATG GACAGAATAA CACTGAAAAC ATGGTGGACA AACTTGGAGC AGATGCAAAA 
ATACTTAGCA CTGTATATAA GCAACACATG GCACGGCCCA AGACCCTGGA CTTAAATAAA 
ATGAATCCCA GAAAGTCAAA GGAGGTTGTT GAAAAAGAAT CTAGTGATGA TGAGTCTCCC 
ATAGGAGGAG CAGATGACAA AGTGGACTCC CCTGTTATTT TTGACCTTGA AGATCTAGAT 
AATGATTCTG ATGAACTTAA ATCAGCAAAA CCATGTGTCC GGAAGGCCAA ACTTGCTCAA 
AGAAGTATCA GCATGCCCTT TTCATCTTCC ATGTTTCTGG CTAAGCCAGC TGAAAAAACA 
GACGTGAAGG CTGGATTAGA CCCTTTGTCA TTGCTGGCAG CTGAAACAGA ACAGGCAGAA 
GTAGAACAGG AGGAGGAAGA TGACAAAAGC TTACTAACAC CATCTGCTAA AAGGGACCTT 
GCAGAGGAAA TAGAAATGTA CATGAACAAT ATAAGCAGCC CCCTTGCAAG TCGAACCCCT 
AGCATGGACC TTGACAGATC CAGTTCTGAA CTAAATAAAA CATGTAAAGC TGTAGCACTC 
AACAGGCGCT TGAGTGTTCC TGCTAGATCC CCACAAGAAC CAAGACTAAC CAGATCAATT 
ACTGCCCACA GTAAACGCGA CAAAAGACAA AGAAGCAGGC TCTGGTCTTC TCCTGCTGTT 
TCCGAGAACA ATCCTCAGAA TGAGCAAAGC CCAGAATCTG CCCTTGCTTT AATGTCCCCA 
ACATCATTTA ACATAGATAC ACTTCTCACG CCAACCTTTG ATGTTCTTAG GAGCAACATG 
TTCTCAGCTG GCAAAGGAGT AGCCAAGAAG GCCAGCAAAT GGTATTCAAA GATCGCAAAT 
TATGCGGGGC CTGCAAAGGA TCAGAATTCT GACCGAGCCA GTCTGTCTTC AGGAGGAGCA 
GGGGATACTG AATCCTACTC TTTAAATGAT GATGATTTTG ATGGCAGTGC TTCTCTGCAT 
GAGACTGATG TCTTTCCTGG AACCAATACC ATTGGTGTAA GCAGATGCAG CTTGGAGAGT 
AGCACATCTC AAGACAGTAA CTCTAAACAA CTTTACAATA ATGGTCTGCC ATCAACAGCA 
TCTCCAAACT ACAAGAGTGA GATTCTTTCA TCCTGCAATT CCAGTAGCAC TAGTGTCTTC 
CAGAACTATG CTATGGAGGT TCTGATATCT AGCTGTTCAC GTTGCTTGAC CTGTGACGGT 
CTTGTTCATG ATGAGGAGAT TATGGCAGGC TGGACGGCAG ATGACTCAAA CCTTAACACC 
ACATGTCCTT TTTGCGGGAA CTCATTTCTT CCATCTTTAA ACGTTGAAAT TAGGGACCTG 
CGACGCCCTG GAAGGTACTT TCTGAAGGCC AGCCCATCTG CAGAAAGTAT GCCGCATTCT 
TCAGTATCCT CACAAAGACC TAACTCAGAT ATTCTGACTC CGGCTTCTCC CTGTGCTTCC 
ACCAAAGTCA AGGATTTTAG TTTGTGCAGT AAAACCAAAC AAGAGAGAAC TCGGTCTGTA 
ACTGTTGAGA TCCCAGTGGG GAAGAGAACC AGAGATCTCA ATGGGTCCTT ATGCCCGAAT 
CAGCCCATTG CACGAAGTAT CAGCACATTT GGTCCCTTAG ATGAACAGCC ATCTCATGAC 
CAGAGCCATG TTATTGCCAC TGGCAGCCTC CCAACTTGCT TCCAGGCACC TCCTGAGTCA 
GGATTGGAAT GGAAGATGTC AAATCCAGAG CCAATCACAG TCCCTTACCT TAGCCCGCTG 
GTTGTCTGGA AGGAGCTGGA GAGCCTGCTA GAAAATGAGG GTGACCAATC GATTACAGTT 
TCTGGATTTG TGGACCATCA TCCGATAGTA TTCTGGAATT TGGTGTGGTA TTTCAGGAGG 
CTGGATCTCC CAAGTAACCT CCCGGGATTA ATTCTGTCTT CGGAACAATG CAATAAAAAT 
TCAAAGGTCT CCAGGAGCTG TATGTCTGCA GACAGCAAAC ATGTCCTTAT TCAAGTGCTG 
TGGGACAATC TGAAATTGCA CCAGGATCCC CGGCAGCCTC TCTACATCCT CTGGAACACT 
ATTAGTCAAA ACCGCACTGT ATTGTTTGAT ACACAGGAAC ATGCGCTTGC TGTGCCACAG 
ATCAAAGAGG AGGAATTGTT TGATCAGCAG TTTCTCAGAG GGATAGTAAG AAGCATAAAG 
AACAACAATG TTTATGGTCC TGTGAGCCAG ATATTGGACT GTATTAAACA GGTCCCTGGA 
AGAACGAGGC AGAGGAGTCT GTACAGGGAA ATTCTTTTTC TTTCTCTTGT TGCCTTGGGA 
AGAGAGAATA TCGATATTGA TGTATTTGAT CGGGAATATA AAACTGCATA CGAGCGCCTC 
ACCCCTGAGC AGCTGAACTG CACACAGATT TGTGATCGGC CTCCCAGCAT TGGAGTGATG 
GAGTGCAGGA AGACTTTCGG ACAGCCATTC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4a ( XM_018092498.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947987.1 Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X2, mRNA.

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CloneID	OXa43407
Clone ID Related Accession (Same CDS sequence)	XM_018092498.1
Accession Version	XM_018092498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	C-myc promoter-binding protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCTGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGCTTT TCTCAGAGAG GAATAATGCA 
GATAAAGGTG GCAAGGCGGA TGTGGATCGA CCAGGAGAGA CACGTCTTAT TGAACTGGAT 
GAGTCTCACA GAAGTGAGCA TACAGTGTTC ATCACACCAC CAGAGATTCC CCACTTACCA 
AATGGCGAGG AGCCCCCTAA GAACTATAGC TACAATGGGT TCCCTGTCCT TATCTTGGAT 
CTCTTTGACA CGCCAGATGG ACTCATGAAA AGTCACAAAC TTAGTACAAA AGTCACCACA 
CCCTGCAGTC CTTCTCCAAT GTTTAGAAGA ACAAAACAGG AAATCCGGTC AGCACATAAA 
ATAGCAAAGC AGTACTCCTC CATCCCCCAG ATGTGGTCCA GGTGTCTACT GAGACATTGC 
TATGGACTTT GGTTTATCTG TCTGCCCGCA TATGTGAAAT TCTGCCATTC CAAAGTGAGA 
GCACTGCGCA CAGCCTACGA TGTGTTGCGA AAAATGCACA TGAAAAAGAT TGACTCTCCT 
GATGAGGTTT GCTATAGGGT TCTAATGCAG TTGTGTGGCC AATATGGGCA GCCTGTCCTT 
GCTGTTAAAG TTCTCTTTGA AATGAGAAAG GTCGGCGTGC AGCCAAATGC TATAACATAT 
GGCTATTATA ATAAGGCTGT CCTGGAAAGC GTATGGCCCT CTGGGAATAG AGGGGGTTAC 
TTTCTGTGGA TAAAGGTGAG GAATGTGGTG CTAGCAGTAG CCCAGTTCAA ACAGGCTTTG 
ATAAAGCAAC ACGCTTCAGC TCATCAAACT CCTGCTTCAG ATTGCAGTGA TTTGGACGCA 
GTCAGCCATG GCAGTTTGGA TAGTTCTGTT GACATGAACC CTACAGAACA GCCTTCATTC 
AGTACAGATG TAACATGTGC TGATCCAGCT GACGACCGAT CTAGTACAGG AGGCCAGTCA 
GACCTTGGCT ACAGTTCATT ATCAAAAGAA GAAGTAAGAA GAGGAGCCGT GCATGACATA 
CAGGAGGAGA AGGAAGACTC CAGAGATAGT GACTCCAGTT CGTCATCTGA TTCAGAAAGT 
TCCAAGAGCA GCAGAGACTG TGTGCCACAA GGAACTGGCA GTCAGGGTCA ACAAACATTG 
GCAAGCACAT CTAGTATAGT CCGGAAAGAA GCAAAATCAG ACAGTGCAAG CAGTGACAAC 
TCAGCAGGTC TACTGTTCAC ACCATCCTTT AGTGAGAATT GTGAAAGTGA AGTGTATCAG 
TGCTGGAGTC TACATAAAAG ACACAAGAGT CCTGTGGAGT CAGATCTGAA AAAACAGATG 
GAATGGGAAA AGAGGAACAG GCATCTAAGC AGTGGCAATG TTGAGGAATT ATTTATCAGT 
AAGTCTGATG GACAGAATAA CACTGAAAAC ATGGTGGACA AACTTGGAGC AGATGCAAAA 
ATACTTAGCA CTGTATATAA GCAACACATG GCACGGCCCA AGACCCTGGA CTTAAATAAA 
ATGAATCCCA GAAAGTCAAA GGAGGTTGTT GAAAAAGAAT CTAGTGATGA TGAGTCTCCC 
ATAGGAGGAG CAGATGACAA AGTGGACTCC CCTGTTATTT TTGACCTTGA AGATCTAGAT 
AATGATTCTG ATGAACTTAA ATCAGCAAAA CCATGTGTCC GGAAGGCCAA ACTTGCTCAA 
AGAAGTATCA GCATGCCCTT TTCATCTTCC ATGTTTCTGG CTAAGCCAGC TGAAAAAACA 
GACGTGAAGG CTGGATTAGA CCCTTTGTCA TTGCTGGCAG CTGAAACAGA ACAGGCAGAA 
GTAGAACAGG AGGAGGAAGA TGACAAAAGC TTACTAACAC CATCTGCTAA AAGGGACCTT 
GCAGAGGAAA TAGAAATGTA CATGAACAAT ATAAGCAGCC CCCTTGCAAG TCGAACCCCT 
AGCATGGACC TTGACAGATC CAGTTCTGAA CTAAATAAAA CATGTAAAGC TGTAGCACTC 
AACAGGCGCT TGAGTGTTCC TGCTAGATCC CCACAAGAAC CAAGACTAAC CAGATCAATT 
ACTGCCCACA GTAAACGCGA CAAAAGACAA AGAAGCAGGC TCTGGTCTTC TCCTGCTGTT 
TCCGAGAACA ATCCTCAGAA TGAGCAAAGC CCAGAATCTG CCCTTGCTTT AATGTCCCCA 
ACATCATTTA ACATAGATAC ACTTCTCACG CCAACCTTTG ATGTTCTTAG GAGCAACATG 
TTCTCAGCTG GCAAAGGAGT AGCCAAGAAG GCCAGCAAAT GGTATTCAAA GATCGCAAAT 
TATGCGGGGC CTGCAAAGGA TCAGAATTCT GACCGAGCCA GTCTGTCTTC AGGAGGAGCA 
GGGGATACTG AATCCTACTC TTTAAATGAT GATGATTTTG ATGGCAGTGC TTCTCTGCAT 
GAGACTGATG TCTTTCCTGG AACCAATACC ATTGGTGTAA GCAGATGCAG CTTGGAGAGT 
AGCACATCTC AAGACAGTAA CTCTAAACAA CTTTACAATA ATGGTCTGCC ATCAACAGCA 
TCTCCAAACT ACAAGAGTGA GATTCTTTCA TCCTGCAATT CCAGTAGCAC TAGTGTCTTC 
CAGAACTATG CTATGGAGGT TCTGATATCT AGCTGTTCAC GTTGCTTGAC CTGTGACGGT 
CTTGTTCATG ATGAGGAGAT TATGGCAGGC TGGACGGCAG ATGACTCAAA CCTTAACACC 
ACATGTCCTT TTTGCGGGAA CTCATTTCTT CCATCTTTAA ACGTTGAAAT TAGGGACCTG 
CGACGCCCTG GAAGGTACTT TCTGAAGGCC AGCCCATCTG CAGAAAGTAT GCCGCATTCT 
TCAGTATCCT CACAAAGACC TAACTCAGAT ATTCTGACTC CGGCTTCTCC CTGTGCTTCC 
ACCAAAGTCA AGGATTTTAG TTTGTGCAGT AAAACCAAAC AAGAGAGAAC TCGGTCTGTA 
ACTGTTGAGA TCCCAGTGGG GAAGAGAACC AGAGATCTCA ATGGGTCCTT ATGCCCGAAT 
CAGCCCATTG CACGAAGTAT CAGCACATTT GGTCCCTTAG ATGAACAGCC ATCTCATGAC 
CAGAGCCATG TTATTGCCAC TGGCAGCCTC CCAACTTGCT TCCAGGCACC TCCTGAGTCA 
GGATTGGAAT GGAAGATGTC AAATCCAGAG CCAATCACAG TCCCTTACCT TAGCCCGCTG 
GTTGTCTGGA AGGAGCTGGA GAGCCTGCTA GAAAATGAGG GTGACCAATC GATTACAGTT 
TCTGGATTTG TGGACCATCA TCCGATAGTA TTCTGGAATT TGGTGTGGTA TTTCAGGAGG 
CTGGATCTCC CAAGTAACCT CCCGGGATTA ATTCTGTCTT CGGAACAATG CAATAAAAAT 
TCAAAGGTCT CCAGGAGCTG TATGTCTGCA GACAGCAAAC ATGTCCTTAT TCAAGTGCTG 
TGGGACAATC TGAAATTGCA CCAGGATCCC CGGCAGCCTC TCTACATCCT CTGGAACACT 
ATTACACAGG AACATGCGCT TGCTGTGCCA CAGATCAAAG AGGAGGAATT GTTTGATCAG 
CAGTTTCTCA GAGGGATAGT AAGAAGCATA AAGAACAACA ATGTTTATGG TCCTGTGAGC 
CAGATATTGG ACTGTATTAA ACAGGTCCCT GGAAGAACGA GGCAGAGGAG TCTGTACAGG 
GAAATTCTTT TTCTTTCTCT TGTTGCCTTG GGAAGAGAGA ATATCGATAT TGATGTATTT 
GATCGGGAAT ATAAAACTGC ATACGAGCGC CTCACCCCTG AGCAGCTGAA CTGCACACAG 
ATTTGTGATC GGCCTCCCAG CATTGGAGTG ATGGAGTGCA GGAAGACTTT CGGACAGCCA 
TTCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947987.1
CDS	27..5795
Translation	MMEDKGPRVADYFVVTGLTDSSKPLEEEIHFNDACHKTAKPKEPITDVTVIIKSLGEEVPQGYVCIDLTPTGLSADLNNGSIRGPQIYLCYRRGRDKPPLTDLGVLYEWKERLKQGCKIIQATPYGRPANISGSASSQRVFITYRRAAESMTQNSLAVTDICIIIPSKGETPPHTFCKVEKNLNSSVWGSAVYLCYKKSVAKTNAVSYKAGLICRYPEEDYESFPLPESVPLFCLPMGATIECWPSDSNYPLPVFSTFVLTGASAEKVYGAAIQFYEPYAQDQLTEKQQVHLGLLSSAEGQMDSSKSIHVNKCICLLSHWPFFDAFKKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDTLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQIEGKRNVTWKILPKKACKNLLNTLCNLYQQLAELQHRPREEAFMELAMNDYDFNYGKRMHQLDMEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFELQGFLKSRDRSHHKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVEKLFSERNNADKGGKADVDRPGETRLIELDESHRSEHTVFITPPEIPHLPNGEEPPKNYSYNGFPVLILDLFDTPDGLMKSHKLSTKVTTPCSPSPMFRRTKQEIRSAHKIAKQYSSIPQMWSRCLLRHCYGLWFICLPAYVKFCHSKVRALRTAYDVLRKMHMKKIDSPDEVCYRVLMQLCGQYGQPVLAVKVLFEMRKVGVQPNAITYGYYNKAVLESVWPSGNRGGYFLWIKVRNVVLAVAQFKQALIKQHASAHQTPASDCSDLDAVSHGSLDSSVDMNPTEQPSFSTDVTCADPADDRSSTGGQSDLGYSSLSKEEVRRGAVHDIQEEKEDSRDSDSSSSSDSESSKSSRDCVPQGTGSQGQQTLASTSSIVRKEAKSDSASSDNSAGLLFTPSFSENCESEVYQCWSLHKRHKSPVESDLKKQMEWEKRNRHLSSGNVEELFISKSDGQNNTENMVDKLGADAKILSTVYKQHMARPKTLDLNKMNPRKSKEVVEKESSDDESPIGGADDKVDSPVIFDLEDLDNDSDELKSAKPCVRKAKLAQRSISMPFSSSMFLAKPAEKTDVKAGLDPLSLLAAETEQAEVEQEEEDDKSLLTPSAKRDLAEEIEMYMNNISSPLASRTPSMDLDRSSSELNKTCKAVALNRRLSVPARSPQEPRLTRSITAHSKRDKRQRSRLWSSPAVSENNPQNEQSPESALALMSPTSFNIDTLLTPTFDVLRSNMFSAGKGVAKKASKWYSKIANYAGPAKDQNSDRASLSSGGAGDTESYSLNDDDFDGSASLHETDVFPGTNTIGVSRCSLESSTSQDSNSKQLYNNGLPSTASPNYKSEILSSCNSSSTSVFQNYAMEVLISSCSRCLTCDGLVHDEEIMAGWTADDSNLNTTCPFCGNSFLPSLNVEIRDLRRPGRYFLKASPSAESMPHSSVSSQRPNSDILTPASPCASTKVKDFSLCSKTKQERTRSVTVEIPVGKRTRDLNGSLCPNQPIARSISTFGPLDEQPSHDQSHVIATGSLPTCFQAPPESGLEWKMSNPEPITVPYLSPLVVWKELESLLENEGDQSITVSGFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEQCNKNSKVSRSCMSADSKHVLIQVLWDNLKLHQDPRQPLYILWNTITQEHALAVPQIKEEELFDQQFLRGIVRSIKNNNVYGPVSQILDCIKQVPGRTRQRSLYREILFLSLVALGRENIDIDVFDREYKTAYERLTPEQLNCTQICDRPPSIGVMECRKTFGQPFL

Target ORF information:

RefSeq Version	XM_018092498.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092498.1

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCTGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGCTTT TCTCAGAGAG GAATAATGCA 
GATAAAGGTG GCAAGGCGGA TGTGGATCGA CCAGGAGAGA CACGTCTTAT TGAACTGGAT 
GAGTCTCACA GAAGTGAGCA TACAGTGTTC ATCACACCAC CAGAGATTCC CCACTTACCA 
AATGGCGAGG AGCCCCCTAA GAACTATAGC TACAATGGGT TCCCTGTCCT TATCTTGGAT 
CTCTTTGACA CGCCAGATGG ACTCATGAAA AGTCACAAAC TTAGTACAAA AGTCACCACA 
CCCTGCAGTC CTTCTCCAAT GTTTAGAAGA ACAAAACAGG AAATCCGGTC AGCACATAAA 
ATAGCAAAGC AGTACTCCTC CATCCCCCAG ATGTGGTCCA GGTGTCTACT GAGACATTGC 
TATGGACTTT GGTTTATCTG TCTGCCCGCA TATGTGAAAT TCTGCCATTC CAAAGTGAGA 
GCACTGCGCA CAGCCTACGA TGTGTTGCGA AAAATGCACA TGAAAAAGAT TGACTCTCCT 
GATGAGGTTT GCTATAGGGT TCTAATGCAG TTGTGTGGCC AATATGGGCA GCCTGTCCTT 
GCTGTTAAAG TTCTCTTTGA AATGAGAAAG GTCGGCGTGC AGCCAAATGC TATAACATAT 
GGCTATTATA ATAAGGCTGT CCTGGAAAGC GTATGGCCCT CTGGGAATAG AGGGGGTTAC 
TTTCTGTGGA TAAAGGTGAG GAATGTGGTG CTAGCAGTAG CCCAGTTCAA ACAGGCTTTG 
ATAAAGCAAC ACGCTTCAGC TCATCAAACT CCTGCTTCAG ATTGCAGTGA TTTGGACGCA 
GTCAGCCATG GCAGTTTGGA TAGTTCTGTT GACATGAACC CTACAGAACA GCCTTCATTC 
AGTACAGATG TAACATGTGC TGATCCAGCT GACGACCGAT CTAGTACAGG AGGCCAGTCA 
GACCTTGGCT ACAGTTCATT ATCAAAAGAA GAAGTAAGAA GAGGAGCCGT GCATGACATA 
CAGGAGGAGA AGGAAGACTC CAGAGATAGT GACTCCAGTT CGTCATCTGA TTCAGAAAGT 
TCCAAGAGCA GCAGAGACTG TGTGCCACAA GGAACTGGCA GTCAGGGTCA ACAAACATTG 
GCAAGCACAT CTAGTATAGT CCGGAAAGAA GCAAAATCAG ACAGTGCAAG CAGTGACAAC 
TCAGCAGGTC TACTGTTCAC ACCATCCTTT AGTGAGAATT GTGAAAGTGA AGTGTATCAG 
TGCTGGAGTC TACATAAAAG ACACAAGAGT CCTGTGGAGT CAGATCTGAA AAAACAGATG 
GAATGGGAAA AGAGGAACAG GCATCTAAGC AGTGGCAATG TTGAGGAATT ATTTATCAGT 
AAGTCTGATG GACAGAATAA CACTGAAAAC ATGGTGGACA AACTTGGAGC AGATGCAAAA 
ATACTTAGCA CTGTATATAA GCAACACATG GCACGGCCCA AGACCCTGGA CTTAAATAAA 
ATGAATCCCA GAAAGTCAAA GGAGGTTGTT GAAAAAGAAT CTAGTGATGA TGAGTCTCCC 
ATAGGAGGAG CAGATGACAA AGTGGACTCC CCTGTTATTT TTGACCTTGA AGATCTAGAT 
AATGATTCTG ATGAACTTAA ATCAGCAAAA CCATGTGTCC GGAAGGCCAA ACTTGCTCAA 
AGAAGTATCA GCATGCCCTT TTCATCTTCC ATGTTTCTGG CTAAGCCAGC TGAAAAAACA 
GACGTGAAGG CTGGATTAGA CCCTTTGTCA TTGCTGGCAG CTGAAACAGA ACAGGCAGAA 
GTAGAACAGG AGGAGGAAGA TGACAAAAGC TTACTAACAC CATCTGCTAA AAGGGACCTT 
GCAGAGGAAA TAGAAATGTA CATGAACAAT ATAAGCAGCC CCCTTGCAAG TCGAACCCCT 
AGCATGGACC TTGACAGATC CAGTTCTGAA CTAAATAAAA CATGTAAAGC TGTAGCACTC 
AACAGGCGCT TGAGTGTTCC TGCTAGATCC CCACAAGAAC CAAGACTAAC CAGATCAATT 
ACTGCCCACA GTAAACGCGA CAAAAGACAA AGAAGCAGGC TCTGGTCTTC TCCTGCTGTT 
TCCGAGAACA ATCCTCAGAA TGAGCAAAGC CCAGAATCTG CCCTTGCTTT AATGTCCCCA 
ACATCATTTA ACATAGATAC ACTTCTCACG CCAACCTTTG ATGTTCTTAG GAGCAACATG 
TTCTCAGCTG GCAAAGGAGT AGCCAAGAAG GCCAGCAAAT GGTATTCAAA GATCGCAAAT 
TATGCGGGGC CTGCAAAGGA TCAGAATTCT GACCGAGCCA GTCTGTCTTC AGGAGGAGCA 
GGGGATACTG AATCCTACTC TTTAAATGAT GATGATTTTG ATGGCAGTGC TTCTCTGCAT 
GAGACTGATG TCTTTCCTGG AACCAATACC ATTGGTGTAA GCAGATGCAG CTTGGAGAGT 
AGCACATCTC AAGACAGTAA CTCTAAACAA CTTTACAATA ATGGTCTGCC ATCAACAGCA 
TCTCCAAACT ACAAGAGTGA GATTCTTTCA TCCTGCAATT CCAGTAGCAC TAGTGTCTTC 
CAGAACTATG CTATGGAGGT TCTGATATCT AGCTGTTCAC GTTGCTTGAC CTGTGACGGT 
CTTGTTCATG ATGAGGAGAT TATGGCAGGC TGGACGGCAG ATGACTCAAA CCTTAACACC 
ACATGTCCTT TTTGCGGGAA CTCATTTCTT CCATCTTTAA ACGTTGAAAT TAGGGACCTG 
CGACGCCCTG GAAGGTACTT TCTGAAGGCC AGCCCATCTG CAGAAAGTAT GCCGCATTCT 
TCAGTATCCT CACAAAGACC TAACTCAGAT ATTCTGACTC CGGCTTCTCC CTGTGCTTCC 
ACCAAAGTCA AGGATTTTAG TTTGTGCAGT AAAACCAAAC AAGAGAGAAC TCGGTCTGTA 
ACTGTTGAGA TCCCAGTGGG GAAGAGAACC AGAGATCTCA ATGGGTCCTT ATGCCCGAAT 
CAGCCCATTG CACGAAGTAT CAGCACATTT GGTCCCTTAG ATGAACAGCC ATCTCATGAC 
CAGAGCCATG TTATTGCCAC TGGCAGCCTC CCAACTTGCT TCCAGGCACC TCCTGAGTCA 
GGATTGGAAT GGAAGATGTC AAATCCAGAG CCAATCACAG TCCCTTACCT TAGCCCGCTG 
GTTGTCTGGA AGGAGCTGGA GAGCCTGCTA GAAAATGAGG GTGACCAATC GATTACAGTT 
TCTGGATTTG TGGACCATCA TCCGATAGTA TTCTGGAATT TGGTGTGGTA TTTCAGGAGG 
CTGGATCTCC CAAGTAACCT CCCGGGATTA ATTCTGTCTT CGGAACAATG CAATAAAAAT 
TCAAAGGTCT CCAGGAGCTG TATGTCTGCA GACAGCAAAC ATGTCCTTAT TCAAGTGCTG 
TGGGACAATC TGAAATTGCA CCAGGATCCC CGGCAGCCTC TCTACATCCT CTGGAACACT 
ATTACACAGG AACATGCGCT TGCTGTGCCA CAGATCAAAG AGGAGGAATT GTTTGATCAG 
CAGTTTCTCA GAGGGATAGT AAGAAGCATA AAGAACAACA ATGTTTATGG TCCTGTGAGC 
CAGATATTGG ACTGTATTAA ACAGGTCCCT GGAAGAACGA GGCAGAGGAG TCTGTACAGG 
GAAATTCTTT TTCTTTCTCT TGTTGCCTTG GGAAGAGAGA ATATCGATAT TGATGTATTT 
GATCGGGAAT ATAAAACTGC ATACGAGCGC CTCACCCCTG AGCAGCTGAA CTGCACACAG 
ATTTGTGATC GGCCTCCCAG CATTGGAGTG ATGGAGTGCA GGAAGACTTT CGGACAGCCA 
TTCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4a ( XM_018092499.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947988.1 Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X3, mRNA.

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CloneID	OXa43408
Clone ID Related Accession (Same CDS sequence)	XM_018092499.1
Accession Version	XM_018092499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	C-myc promoter-binding protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCTGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGGCGG ATGTGGATCG ACCAGGAGAG 
ACACGTCTTA TTGAACTGGA TGAGTCTCAC AGAAGTGAGC ATACAGTGTT CATCACACCA 
CCAGAGATTC CCCACTTACC AAATGGCGAG GAGCCCCCTA AGAACTATAG CTACAATGGG 
TTCCCTGTCC TTATCTTGGA TCTCTTTGAC ACGCCAGATG GACTCATGAA AAGTCACAAA 
CTTAGTACAA AAGTCACCAC ACCCTGCAGT CCTTCTCCAA TGTTTAGAAG AACAAAACAG 
GAAATCCGGT CAGCACATAA AATAGCAAAG CAGTACTCCT CCATCCCCCA GATGTGGTCC 
AGGTGTCTAC TGAGACATTG CTATGGACTT TGGTTTATCT GTCTGCCCGC ATATGTGAAA 
TTCTGCCATT CCAAAGTGAG AGCACTGCGC ACAGCCTACG ATGTGTTGCG AAAAATGCAC 
ATGAAAAAGA TTGACTCTCC TGATGAGGTT TGCTATAGGG TTCTAATGCA GTTGTGTGGC 
CAATATGGGC AGCCTGTCCT TGCTGTTAAA GTTCTCTTTG AAATGAGAAA GGTCGGCGTG 
CAGCCAAATG CTATAACATA TGGCTATTAT AATAAGGCTG TCCTGGAAAG CGTATGGCCC 
TCTGGGAATA GAGGGGGTTA CTTTCTGTGG ATAAAGGTGA GGAATGTGGT GCTAGCAGTA 
GCCCAGTTCA AACAGGCTTT GATAAAGCAA CACGCTTCAG CTCATCAAAC TCCTGCTTCA 
GATTGCAGTG ATTTGGACGC AGTCAGCCAT GGCAGTTTGG ATAGTTCTGT TGACATGAAC 
CCTACAGAAC AGCCTTCATT CAGTACAGAT GTAACATGTG CTGATCCAGC TGACGACCGA 
TCTAGTACAG GAGGCCAGTC AGACCTTGGC TACAGTTCAT TATCAAAAGA AGAAGTAAGA 
AGAGGAGCCG TGCATGACAT ACAGGAGGAG AAGGAAGACT CCAGAGATAG TGACTCCAGT 
TCGTCATCTG ATTCAGAAAG TTCCAAGAGC AGCAGAGACT GTGTGCCACA AGGAACTGGC 
AGTCAGGGTC AACAAACATT GGCAAGCACA TCTAGTATAG TCCGGAAAGA AGCAAAATCA 
GACAGTGCAA GCAGTGACAA CTCAGCAGGT CTACTGTTCA CACCATCCTT TAGTGAGAAT 
TGTGAAAGTG AAGTGTATCA GTGCTGGAGT CTACATAAAA GACACAAGAG TCCTGTGGAG 
TCAGATCTGA AAAAACAGAT GGAATGGGAA AAGAGGAACA GGCATCTAAG CAGTGGCAAT 
GTTGAGGAAT TATTTATCAG TAAGTCTGAT GGACAGAATA ACACTGAAAA CATGGTGGAC 
AAACTTGGAG CAGATGCAAA AATACTTAGC ACTGTATATA AGCAACACAT GGCACGGCCC 
AAGACCCTGG ACTTAAATAA AATGAATCCC AGAAAGTCAA AGGAGGTTGT TGAAAAAGAA 
TCTAGTGATG ATGAGTCTCC CATAGGAGGA GCAGATGACA AAGTGGACTC CCCTGTTATT 
TTTGACCTTG AAGATCTAGA TAATGATTCT GATGAACTTA AATCAGCAAA ACCATGTGTC 
CGGAAGGCCA AACTTGCTCA AAGAAGTATC AGCATGCCCT TTTCATCTTC CATGTTTCTG 
GCTAAGCCAG CTGAAAAAAC AGACGTGAAG GCTGGATTAG ACCCTTTGTC ATTGCTGGCA 
GCTGAAACAG AACAGGCAGA AGTAGAACAG GAGGAGGAAG ATGACAAAAG CTTACTAACA 
CCATCTGCTA AAAGGGACCT TGCAGAGGAA ATAGAAATGT ACATGAACAA TATAAGCAGC 
CCCCTTGCAA GTCGAACCCC TAGCATGGAC CTTGACAGAT CCAGTTCTGA ACTAAATAAA 
ACATGTAAAG CTGTAGCACT CAACAGGCGC TTGAGTGTTC CTGCTAGATC CCCACAAGAA 
CCAAGACTAA CCAGATCAAT TACTGCCCAC AGTAAACGCG ACAAAAGACA AAGAAGCAGG 
CTCTGGTCTT CTCCTGCTGT TTCCGAGAAC AATCCTCAGA ATGAGCAAAG CCCAGAATCT 
GCCCTTGCTT TAATGTCCCC AACATCATTT AACATAGATA CACTTCTCAC GCCAACCTTT 
GATGTTCTTA GGAGCAACAT GTTCTCAGCT GGCAAAGGAG TAGCCAAGAA GGCCAGCAAA 
TGGTATTCAA AGATCGCAAA TTATGCGGGG CCTGCAAAGG ATCAGAATTC TGACCGAGCC 
AGTCTGTCTT CAGGAGGAGC AGGGGATACT GAATCCTACT CTTTAAATGA TGATGATTTT 
GATGGCAGTG CTTCTCTGCA TGAGACTGAT GTCTTTCCTG GAACCAATAC CATTGGTGTA 
AGCAGATGCA GCTTGGAGAG TAGCACATCT CAAGACAGTA ACTCTAAACA ACTTTACAAT 
AATGGTCTGC CATCAACAGC ATCTCCAAAC TACAAGAGTG AGATTCTTTC ATCCTGCAAT 
TCCAGTAGCA CTAGTGTCTT CCAGAACTAT GCTATGGAGG TTCTGATATC TAGCTGTTCA 
CGTTGCTTGA CCTGTGACGG TCTTGTTCAT GATGAGGAGA TTATGGCAGG CTGGACGGCA 
GATGACTCAA ACCTTAACAC CACATGTCCT TTTTGCGGGA ACTCATTTCT TCCATCTTTA 
AACGTTGAAA TTAGGGACCT GCGACGCCCT GGAAGGTACT TTCTGAAGGC CAGCCCATCT 
GCAGAAAGTA TGCCGCATTC TTCAGTATCC TCACAAAGAC CTAACTCAGA TATTCTGACT 
CCGGCTTCTC CCTGTGCTTC CACCAAAGTC AAGGATTTTA GTTTGTGCAG TAAAACCAAA 
CAAGAGAGAA CTCGGTCTGT AACTGTTGAG ATCCCAGTGG GGAAGAGAAC CAGAGATCTC 
AATGGGTCCT TATGCCCGAA TCAGCCCATT GCACGAAGTA TCAGCACATT TGGTCCCTTA 
GATGAACAGC CATCTCATGA CCAGAGCCAT GTTATTGCCA CTGGCAGCCT CCCAACTTGC 
TTCCAGGCAC CTCCTGAGTC AGGATTGGAA TGGAAGATGT CAAATCCAGA GCCAATCACA 
GTCCCTTACC TTAGCCCGCT GGTTGTCTGG AAGGAGCTGG AGAGCCTGCT AGAAAATGAG 
GGTGACCAAT CGATTACAGT TTCTGGATTT GTGGACCATC ATCCGATAGT ATTCTGGAAT 
TTGGTGTGGT ATTTCAGGAG GCTGGATCTC CCAAGTAACC TCCCGGGATT AATTCTGTCT 
TCGGAACAAT GCAATAAAAA TTCAAAGGTC TCCAGGAGCT GTATGTCTGC AGACAGCAAA 
CATGTCCTTA TTCAAGTGCT GTGGGACAAT CTGAAATTGC ACCAGGATCC CCGGCAGCCT 
CTCTACATCC TCTGGAACAC TATTAGTCAA AACCGCACTG TATTGTTTGA TACACAGGAA 
CATGCGCTTG CTGTGCCACA GATCAAAGAG GAGGAATTGT TTGATCAGCA GTTTCTCAGA 
GGGATAGTAA GAAGCATAAA GAACAACAAT GTTTATGGTC CTGTGAGCCA GATATTGGAC 
TGTATTAAAC AGGTCCCTGG AAGAACGAGG CAGAGGAGTC TGTACAGGGA AATTCTTTTT 
CTTTCTCTTG TTGCCTTGGG AAGAGAGAAT ATCGATATTG ATGTATTTGA TCGGGAATAT 
AAAACTGCAT ACGAGCGCCT CACCCCTGAG CAGCTGAACT GCACACAGAT TTGTGATCGG 
CCTCCCAGCA TTGGAGTGAT GGAGTGCAGG AAGACTTTCG GACAGCCATT CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947988.1
CDS	27..5783
Translation	MMEDKGPRVADYFVVTGLTDSSKPLEEEIHFNDACHKTAKPKEPITDVTVIIKSLGEEVPQGYVCIDLTPTGLSADLNNGSIRGPQIYLCYRRGRDKPPLTDLGVLYEWKERLKQGCKIIQATPYGRPANISGSASSQRVFITYRRAAESMTQNSLAVTDICIIIPSKGETPPHTFCKVEKNLNSSVWGSAVYLCYKKSVAKTNAVSYKAGLICRYPEEDYESFPLPESVPLFCLPMGATIECWPSDSNYPLPVFSTFVLTGASAEKVYGAAIQFYEPYAQDQLTEKQQVHLGLLSSAEGQMDSSKSIHVNKCICLLSHWPFFDAFKKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDTLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQIEGKRNVTWKILPKKACKNLLNTLCNLYQQLAELQHRPREEAFMELAMNDYDFNYGKRMHQLDMEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFELQGFLKSRDRSHHKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVEKADVDRPGETRLIELDESHRSEHTVFITPPEIPHLPNGEEPPKNYSYNGFPVLILDLFDTPDGLMKSHKLSTKVTTPCSPSPMFRRTKQEIRSAHKIAKQYSSIPQMWSRCLLRHCYGLWFICLPAYVKFCHSKVRALRTAYDVLRKMHMKKIDSPDEVCYRVLMQLCGQYGQPVLAVKVLFEMRKVGVQPNAITYGYYNKAVLESVWPSGNRGGYFLWIKVRNVVLAVAQFKQALIKQHASAHQTPASDCSDLDAVSHGSLDSSVDMNPTEQPSFSTDVTCADPADDRSSTGGQSDLGYSSLSKEEVRRGAVHDIQEEKEDSRDSDSSSSSDSESSKSSRDCVPQGTGSQGQQTLASTSSIVRKEAKSDSASSDNSAGLLFTPSFSENCESEVYQCWSLHKRHKSPVESDLKKQMEWEKRNRHLSSGNVEELFISKSDGQNNTENMVDKLGADAKILSTVYKQHMARPKTLDLNKMNPRKSKEVVEKESSDDESPIGGADDKVDSPVIFDLEDLDNDSDELKSAKPCVRKAKLAQRSISMPFSSSMFLAKPAEKTDVKAGLDPLSLLAAETEQAEVEQEEEDDKSLLTPSAKRDLAEEIEMYMNNISSPLASRTPSMDLDRSSSELNKTCKAVALNRRLSVPARSPQEPRLTRSITAHSKRDKRQRSRLWSSPAVSENNPQNEQSPESALALMSPTSFNIDTLLTPTFDVLRSNMFSAGKGVAKKASKWYSKIANYAGPAKDQNSDRASLSSGGAGDTESYSLNDDDFDGSASLHETDVFPGTNTIGVSRCSLESSTSQDSNSKQLYNNGLPSTASPNYKSEILSSCNSSSTSVFQNYAMEVLISSCSRCLTCDGLVHDEEIMAGWTADDSNLNTTCPFCGNSFLPSLNVEIRDLRRPGRYFLKASPSAESMPHSSVSSQRPNSDILTPASPCASTKVKDFSLCSKTKQERTRSVTVEIPVGKRTRDLNGSLCPNQPIARSISTFGPLDEQPSHDQSHVIATGSLPTCFQAPPESGLEWKMSNPEPITVPYLSPLVVWKELESLLENEGDQSITVSGFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEQCNKNSKVSRSCMSADSKHVLIQVLWDNLKLHQDPRQPLYILWNTISQNRTVLFDTQEHALAVPQIKEEELFDQQFLRGIVRSIKNNNVYGPVSQILDCIKQVPGRTRQRSLYREILFLSLVALGRENIDIDVFDREYKTAYERLTPEQLNCTQICDRPPSIGVMECRKTFGQPFL

Target ORF information:

RefSeq Version	XM_018092499.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092499.1

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCTGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGGCGG ATGTGGATCG ACCAGGAGAG 
ACACGTCTTA TTGAACTGGA TGAGTCTCAC AGAAGTGAGC ATACAGTGTT CATCACACCA 
CCAGAGATTC CCCACTTACC AAATGGCGAG GAGCCCCCTA AGAACTATAG CTACAATGGG 
TTCCCTGTCC TTATCTTGGA TCTCTTTGAC ACGCCAGATG GACTCATGAA AAGTCACAAA 
CTTAGTACAA AAGTCACCAC ACCCTGCAGT CCTTCTCCAA TGTTTAGAAG AACAAAACAG 
GAAATCCGGT CAGCACATAA AATAGCAAAG CAGTACTCCT CCATCCCCCA GATGTGGTCC 
AGGTGTCTAC TGAGACATTG CTATGGACTT TGGTTTATCT GTCTGCCCGC ATATGTGAAA 
TTCTGCCATT CCAAAGTGAG AGCACTGCGC ACAGCCTACG ATGTGTTGCG AAAAATGCAC 
ATGAAAAAGA TTGACTCTCC TGATGAGGTT TGCTATAGGG TTCTAATGCA GTTGTGTGGC 
CAATATGGGC AGCCTGTCCT TGCTGTTAAA GTTCTCTTTG AAATGAGAAA GGTCGGCGTG 
CAGCCAAATG CTATAACATA TGGCTATTAT AATAAGGCTG TCCTGGAAAG CGTATGGCCC 
TCTGGGAATA GAGGGGGTTA CTTTCTGTGG ATAAAGGTGA GGAATGTGGT GCTAGCAGTA 
GCCCAGTTCA AACAGGCTTT GATAAAGCAA CACGCTTCAG CTCATCAAAC TCCTGCTTCA 
GATTGCAGTG ATTTGGACGC AGTCAGCCAT GGCAGTTTGG ATAGTTCTGT TGACATGAAC 
CCTACAGAAC AGCCTTCATT CAGTACAGAT GTAACATGTG CTGATCCAGC TGACGACCGA 
TCTAGTACAG GAGGCCAGTC AGACCTTGGC TACAGTTCAT TATCAAAAGA AGAAGTAAGA 
AGAGGAGCCG TGCATGACAT ACAGGAGGAG AAGGAAGACT CCAGAGATAG TGACTCCAGT 
TCGTCATCTG ATTCAGAAAG TTCCAAGAGC AGCAGAGACT GTGTGCCACA AGGAACTGGC 
AGTCAGGGTC AACAAACATT GGCAAGCACA TCTAGTATAG TCCGGAAAGA AGCAAAATCA 
GACAGTGCAA GCAGTGACAA CTCAGCAGGT CTACTGTTCA CACCATCCTT TAGTGAGAAT 
TGTGAAAGTG AAGTGTATCA GTGCTGGAGT CTACATAAAA GACACAAGAG TCCTGTGGAG 
TCAGATCTGA AAAAACAGAT GGAATGGGAA AAGAGGAACA GGCATCTAAG CAGTGGCAAT 
GTTGAGGAAT TATTTATCAG TAAGTCTGAT GGACAGAATA ACACTGAAAA CATGGTGGAC 
AAACTTGGAG CAGATGCAAA AATACTTAGC ACTGTATATA AGCAACACAT GGCACGGCCC 
AAGACCCTGG ACTTAAATAA AATGAATCCC AGAAAGTCAA AGGAGGTTGT TGAAAAAGAA 
TCTAGTGATG ATGAGTCTCC CATAGGAGGA GCAGATGACA AAGTGGACTC CCCTGTTATT 
TTTGACCTTG AAGATCTAGA TAATGATTCT GATGAACTTA AATCAGCAAA ACCATGTGTC 
CGGAAGGCCA AACTTGCTCA AAGAAGTATC AGCATGCCCT TTTCATCTTC CATGTTTCTG 
GCTAAGCCAG CTGAAAAAAC AGACGTGAAG GCTGGATTAG ACCCTTTGTC ATTGCTGGCA 
GCTGAAACAG AACAGGCAGA AGTAGAACAG GAGGAGGAAG ATGACAAAAG CTTACTAACA 
CCATCTGCTA AAAGGGACCT TGCAGAGGAA ATAGAAATGT ACATGAACAA TATAAGCAGC 
CCCCTTGCAA GTCGAACCCC TAGCATGGAC CTTGACAGAT CCAGTTCTGA ACTAAATAAA 
ACATGTAAAG CTGTAGCACT CAACAGGCGC TTGAGTGTTC CTGCTAGATC CCCACAAGAA 
CCAAGACTAA CCAGATCAAT TACTGCCCAC AGTAAACGCG ACAAAAGACA AAGAAGCAGG 
CTCTGGTCTT CTCCTGCTGT TTCCGAGAAC AATCCTCAGA ATGAGCAAAG CCCAGAATCT 
GCCCTTGCTT TAATGTCCCC AACATCATTT AACATAGATA CACTTCTCAC GCCAACCTTT 
GATGTTCTTA GGAGCAACAT GTTCTCAGCT GGCAAAGGAG TAGCCAAGAA GGCCAGCAAA 
TGGTATTCAA AGATCGCAAA TTATGCGGGG CCTGCAAAGG ATCAGAATTC TGACCGAGCC 
AGTCTGTCTT CAGGAGGAGC AGGGGATACT GAATCCTACT CTTTAAATGA TGATGATTTT 
GATGGCAGTG CTTCTCTGCA TGAGACTGAT GTCTTTCCTG GAACCAATAC CATTGGTGTA 
AGCAGATGCA GCTTGGAGAG TAGCACATCT CAAGACAGTA ACTCTAAACA ACTTTACAAT 
AATGGTCTGC CATCAACAGC ATCTCCAAAC TACAAGAGTG AGATTCTTTC ATCCTGCAAT 
TCCAGTAGCA CTAGTGTCTT CCAGAACTAT GCTATGGAGG TTCTGATATC TAGCTGTTCA 
CGTTGCTTGA CCTGTGACGG TCTTGTTCAT GATGAGGAGA TTATGGCAGG CTGGACGGCA 
GATGACTCAA ACCTTAACAC CACATGTCCT TTTTGCGGGA ACTCATTTCT TCCATCTTTA 
AACGTTGAAA TTAGGGACCT GCGACGCCCT GGAAGGTACT TTCTGAAGGC CAGCCCATCT 
GCAGAAAGTA TGCCGCATTC TTCAGTATCC TCACAAAGAC CTAACTCAGA TATTCTGACT 
CCGGCTTCTC CCTGTGCTTC CACCAAAGTC AAGGATTTTA GTTTGTGCAG TAAAACCAAA 
CAAGAGAGAA CTCGGTCTGT AACTGTTGAG ATCCCAGTGG GGAAGAGAAC CAGAGATCTC 
AATGGGTCCT TATGCCCGAA TCAGCCCATT GCACGAAGTA TCAGCACATT TGGTCCCTTA 
GATGAACAGC CATCTCATGA CCAGAGCCAT GTTATTGCCA CTGGCAGCCT CCCAACTTGC 
TTCCAGGCAC CTCCTGAGTC AGGATTGGAA TGGAAGATGT CAAATCCAGA GCCAATCACA 
GTCCCTTACC TTAGCCCGCT GGTTGTCTGG AAGGAGCTGG AGAGCCTGCT AGAAAATGAG 
GGTGACCAAT CGATTACAGT TTCTGGATTT GTGGACCATC ATCCGATAGT ATTCTGGAAT 
TTGGTGTGGT ATTTCAGGAG GCTGGATCTC CCAAGTAACC TCCCGGGATT AATTCTGTCT 
TCGGAACAAT GCAATAAAAA TTCAAAGGTC TCCAGGAGCT GTATGTCTGC AGACAGCAAA 
CATGTCCTTA TTCAAGTGCT GTGGGACAAT CTGAAATTGC ACCAGGATCC CCGGCAGCCT 
CTCTACATCC TCTGGAACAC TATTAGTCAA AACCGCACTG TATTGTTTGA TACACAGGAA 
CATGCGCTTG CTGTGCCACA GATCAAAGAG GAGGAATTGT TTGATCAGCA GTTTCTCAGA 
GGGATAGTAA GAAGCATAAA GAACAACAAT GTTTATGGTC CTGTGAGCCA GATATTGGAC 
TGTATTAAAC AGGTCCCTGG AAGAACGAGG CAGAGGAGTC TGTACAGGGA AATTCTTTTT 
CTTTCTCTTG TTGCCTTGGG AAGAGAGAAT ATCGATATTG ATGTATTTGA TCGGGAATAT 
AAAACTGCAT ACGAGCGCCT CACCCCTGAG CAGCTGAACT GCACACAGAT TTGTGATCGG 
CCTCCCAGCA TTGGAGTGAT GGAGTGCAGG AAGACTTTCG GACAGCCATT CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4a ( XM_018092500.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947989.1 Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa43409
Clone ID Related Accession (Same CDS sequence)	XM_018092500.1
Accession Version	XM_018092500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	C-myc promoter-binding protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCTGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGCTTT TCTCAGAGAG GAATAATGCA 
GATAAAGGTG GCAAGGCGGA TGTGGATCGA CCAGGAGAGA CACGTCTTAT TGAACTGGAT 
GAGTCTCACA GAAGTGAGCA TACAGTGTTC ATCACACCAC CAGAGATTCC CCACTTACCA 
AATGGCGAGG AGCCCCCTAA GAACTATAGC TACAATGGGT TCCCTGTCCT TATCTTGGAT 
CTCTTTGACA CGCCAGATGG ACTCATGAAA AGTCACAAAC TTAGTACAAA AGTCACCACA 
CCCTGCAGTC CTTCTCCAAT GTTTAGAAGA ACAAAACAGG AAATCCGGTC AGCACATAAA 
ATAGCAAAGC AGTACTCCTC CATCCCCCAG ATGTGGTCCA GGTGTCTACT GAGACATTGC 
TATGGACTTT GGTTTATCTG TCTGCCCGCA TATGTGAAAT TCTGCCATTC CAAAGTGAGA 
GCACTGCGCA CAGCCTACGA TGTGTTGCGA AAAATGCACA TGAAAAAGAT TGACTCTCCT 
GATGAGGTTT GCTATAGGGT TCTAATGCAG TTGTGTGGCC AATATGGGCA GCCTGTCCTT 
GCTGTTAAAG TTCTCTTTGA AATGAGAAAG GTCGGCGTGC AGCCAAATGC TATAACATAT 
GGCTATTATA ATAAGGCTGT CCTGGAAAGC GTATGGCCCT CTGGGAATAG AGGGGGTTAC 
TTTCTGTGGA TAAAGGTGAG GAATGTGGTG CTAGCAGTAG CCCAGTTCAA ACAGGCTTTG 
ATAAAGCAAC ACGCTTCAGC TCATCAAACT CCTGCTTCAG ATTGCAGTGA TTTGGACGCA 
GTCAGCCATG GCAGTTTGGA TAGTTCTGTT GACATGAACC CTACAGAACA GCCTTCATTC 
AGTACAGATG TAACATGTGC TGATCCAGCT GACGACCGAT CTAGTACAGG AGGCCAGTCA 
GACCTTGGCT ACAGTTCATT ATCAAAAGAA GAAGTAAGAA GAGGAGCCGT GCATGACATA 
CAGGAGGAGA AGGAAGACTC CAGAGATAGT GACTCCAGTT CGTCATCTGA TTCAGAAAGT 
TCCAAGAGCA GCAGAGACTG TGTGCCACAA GGAACTGGCA GTCAGGGTCA ACAAACATTG 
GCAAGCACAT CTAGTATAGT CCGGAAAGAA GCAAAATCAG ACAGTGCAAG CAGTGACAAC 
TCAGCAGGTC TACTGTTCAC ACCATCCTTT AGTGAGAATT GTGAAAGTGA AGTGTATCAG 
TGCTGGAGTC TACATAAAAG ACACAAGAGT CCTGTGGAGT CAGATCTGAA AAAACAGATG 
GAATGGGAAA AGAGGAACAG GCATCTAAGC AGTGGCAATG TTGAGGAATT ATTTATCAGT 
AAGTCTGATG GACAGAATAA CACTGAAAAC ATGGTGGACA AACTTGGAGC AGATGCAAAA 
ATACTTAGCA CTGTATATAA GCAACACATG GCACGGCCCA AGACCCTGGA CTTAAATAAA 
ATGAATCCCA GAAAGTCAAA GGAGGTTGTT GAAAAAGAAT CTAGTGATGA TGAGTCTCCC 
ATAGGAGGAG CAGATGACAA AGTGGACTCC CCTGTTATTT TTGACCTTGA AGATCTAGAT 
AATGATTCTG ATGAACTTAA ATCAGCAAAA CCATGTGTCC GGAAGGCCAA ACTTGCTCAA 
AGAAGTATCA GCATGCCCTT TTCATCTTCC ATGTTTCTGG CTAAGCCAGC TGAAAAAACA 
GACGTGAAGG CTGGATTAGA CCCTTTGTCA TTGCTGGCAG CTGAAACAGA ACAGGCAGAA 
GTAGAACAGG AGGAGGAAGA TGACAAAAGC TTACTAACAC CATCTGCTAA AAGGGACCTT 
GCAGAGGAAA TAGAAATGTA CATGAACAAT ATAAGCAGCC CCCTTGCAAG TCGAACCCCT 
AGCATGGACC TTGACAGATC CAGTTCTGAA CTAAATAAAA CATGTAAAGC TGTAGCACTC 
AACAGGCGCT TGAGTGTTCC TGCTAGATCC CCACAAGAAC CAAGACTAAC CAGATCAATT 
ACTGCCCACA GTAAACGCGA CAAAAGACAA AGAAGCAGGC TCTGGTCTTC TCCTGCTGTT 
TCCGAGAACA ATCCTCAGAA TGAGCAAAGC CCAGAATCTG CCCTTGCTTT AATGTCCCCA 
ACATCATTTA ACATAGATAC ACTTCTCACG CCAACCTTTG ATGTTCTTAG GAGCAACATG 
TTCTCAGCTG GCAAAGGAGT AGCCAAGAAG GCCAGCAAAT GGTATTCAAA GATCGCAAAT 
TATGCGGGGC CTGCAAAGGA TCAGAATTCT GACCGAGCCA GTCTGTCTTC AGGAGGAGCA 
GGGGATACTG AATCCTACTC TTTAAATGAT GATGATTTTG ATGGCAGTGC TTCTCTGCAT 
GAGACTGATG TCTTTCCTGG AACCAATACC ATTGGTGTAA GCAGATGCAG CTTGGAGAGT 
AGCACATCTC AAGACAGTAA CTCTAAACAA CTTTACAATA ATGGTCTGCC ATCAACAGCA 
TCTCCAAACT ACAAGAGTGA GATTCTTTCA TCCTGCAATT CCAGTAGCAC TAGTGTCTTC 
CAGAACTATG CTATGGAGGT TCTGATATCT AGCTGTTCAC GTTGCTTGAC CTGTGACGGT 
CTTGTTCATG ATGAGGAGAT TATGGCAGGC TGGACGGCAG ATGACTCAAA CCTTAACACC 
ACATGTCCTT TTTGCGGGAA CTCATTTCTT CCATCTTTAA ACGTTGAAAT TAGGGACCTG 
CGACGCCCTG GAAGTAAAAC CAAACAAGAG AGAACTCGGT CTGTAACTGT TGAGATCCCA 
GTGGGGAAGA GAACCAGAGA TCTCAATGGG TCCTTATGCC CGAATCAGCC CATTGCACGA 
AGTATCAGCA CATTTGGTCC CTTAGATGAA CAGCCATCTC ATGACCAGAG CCATGTTATT 
GCCACTGGCA GCCTCCCAAC TTGCTTCCAG GCACCTCCTG AGTCAGGATT GGAATGGAAG 
ATGTCAAATC CAGAGCCAAT CACAGTCCCT TACCTTAGCC CGCTGGTTGT CTGGAAGGAG 
CTGGAGAGCC TGCTAGAAAA TGAGGGTGAC CAATCGATTA CAGTTTCTGG ATTTGTGGAC 
CATCATCCGA TAGTATTCTG GAATTTGGTG TGGTATTTCA GGAGGCTGGA TCTCCCAAGT 
AACCTCCCGG GATTAATTCT GTCTTCGGAA CAATGCAATA AAAATTCAAA GGTCTCCAGG 
AGCTGTATGT CTGCAGACAG CAAACATGTC CTTATTCAAG TGCTGTGGGA CAATCTGAAA 
TTGCACCAGG ATCCCCGGCA GCCTCTCTAC ATCCTCTGGA ACACTATTAG TCAAAACCGC 
ACTGTATTGT TTGATACACA GGAACATGCG CTTGCTGTGC CACAGATCAA AGAGGAGGAA 
TTGTTTGATC AGCAGTTTCT CAGAGGGATA GTAAGAAGCA TAAAGAACAA CAATGTTTAT 
GGTCCTGTGA GCCAGATATT GGACTGTATT AAACAGGTCC CTGGAAGAAC GAGGCAGAGG 
AGTCTGTACA GGGAAATTCT TTTTCTTTCT CTTGTTGCCT TGGGAAGAGA GAATATCGAT 
ATTGATGTAT TTGATCGGGA ATATAAAACT GCATACGAGC GCCTCACCCC TGAGCAGCTG 
AACTGCACAC AGATTTGTGA TCGGCCTCCC AGCATTGGAG TGATGGAGTG CAGGAAGACT 
TTCGGACAGC CATTCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947989.1
CDS	27..5687
Translation	MMEDKGPRVADYFVVTGLTDSSKPLEEEIHFNDACHKTAKPKEPITDVTVIIKSLGEEVPQGYVCIDLTPTGLSADLNNGSIRGPQIYLCYRRGRDKPPLTDLGVLYEWKERLKQGCKIIQATPYGRPANISGSASSQRVFITYRRAAESMTQNSLAVTDICIIIPSKGETPPHTFCKVEKNLNSSVWGSAVYLCYKKSVAKTNAVSYKAGLICRYPEEDYESFPLPESVPLFCLPMGATIECWPSDSNYPLPVFSTFVLTGASAEKVYGAAIQFYEPYAQDQLTEKQQVHLGLLSSAEGQMDSSKSIHVNKCICLLSHWPFFDAFKKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDTLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQIEGKRNVTWKILPKKACKNLLNTLCNLYQQLAELQHRPREEAFMELAMNDYDFNYGKRMHQLDMEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFELQGFLKSRDRSHHKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVEKLFSERNNADKGGKADVDRPGETRLIELDESHRSEHTVFITPPEIPHLPNGEEPPKNYSYNGFPVLILDLFDTPDGLMKSHKLSTKVTTPCSPSPMFRRTKQEIRSAHKIAKQYSSIPQMWSRCLLRHCYGLWFICLPAYVKFCHSKVRALRTAYDVLRKMHMKKIDSPDEVCYRVLMQLCGQYGQPVLAVKVLFEMRKVGVQPNAITYGYYNKAVLESVWPSGNRGGYFLWIKVRNVVLAVAQFKQALIKQHASAHQTPASDCSDLDAVSHGSLDSSVDMNPTEQPSFSTDVTCADPADDRSSTGGQSDLGYSSLSKEEVRRGAVHDIQEEKEDSRDSDSSSSSDSESSKSSRDCVPQGTGSQGQQTLASTSSIVRKEAKSDSASSDNSAGLLFTPSFSENCESEVYQCWSLHKRHKSPVESDLKKQMEWEKRNRHLSSGNVEELFISKSDGQNNTENMVDKLGADAKILSTVYKQHMARPKTLDLNKMNPRKSKEVVEKESSDDESPIGGADDKVDSPVIFDLEDLDNDSDELKSAKPCVRKAKLAQRSISMPFSSSMFLAKPAEKTDVKAGLDPLSLLAAETEQAEVEQEEEDDKSLLTPSAKRDLAEEIEMYMNNISSPLASRTPSMDLDRSSSELNKTCKAVALNRRLSVPARSPQEPRLTRSITAHSKRDKRQRSRLWSSPAVSENNPQNEQSPESALALMSPTSFNIDTLLTPTFDVLRSNMFSAGKGVAKKASKWYSKIANYAGPAKDQNSDRASLSSGGAGDTESYSLNDDDFDGSASLHETDVFPGTNTIGVSRCSLESSTSQDSNSKQLYNNGLPSTASPNYKSEILSSCNSSSTSVFQNYAMEVLISSCSRCLTCDGLVHDEEIMAGWTADDSNLNTTCPFCGNSFLPSLNVEIRDLRRPGSKTKQERTRSVTVEIPVGKRTRDLNGSLCPNQPIARSISTFGPLDEQPSHDQSHVIATGSLPTCFQAPPESGLEWKMSNPEPITVPYLSPLVVWKELESLLENEGDQSITVSGFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEQCNKNSKVSRSCMSADSKHVLIQVLWDNLKLHQDPRQPLYILWNTISQNRTVLFDTQEHALAVPQIKEEELFDQQFLRGIVRSIKNNNVYGPVSQILDCIKQVPGRTRQRSLYREILFLSLVALGRENIDIDVFDREYKTAYERLTPEQLNCTQICDRPPSIGVMECRKTFGQPFL

Target ORF information:

RefSeq Version	XM_018092500.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092500.1

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCTGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGCTTT TCTCAGAGAG GAATAATGCA 
GATAAAGGTG GCAAGGCGGA TGTGGATCGA CCAGGAGAGA CACGTCTTAT TGAACTGGAT 
GAGTCTCACA GAAGTGAGCA TACAGTGTTC ATCACACCAC CAGAGATTCC CCACTTACCA 
AATGGCGAGG AGCCCCCTAA GAACTATAGC TACAATGGGT TCCCTGTCCT TATCTTGGAT 
CTCTTTGACA CGCCAGATGG ACTCATGAAA AGTCACAAAC TTAGTACAAA AGTCACCACA 
CCCTGCAGTC CTTCTCCAAT GTTTAGAAGA ACAAAACAGG AAATCCGGTC AGCACATAAA 
ATAGCAAAGC AGTACTCCTC CATCCCCCAG ATGTGGTCCA GGTGTCTACT GAGACATTGC 
TATGGACTTT GGTTTATCTG TCTGCCCGCA TATGTGAAAT TCTGCCATTC CAAAGTGAGA 
GCACTGCGCA CAGCCTACGA TGTGTTGCGA AAAATGCACA TGAAAAAGAT TGACTCTCCT 
GATGAGGTTT GCTATAGGGT TCTAATGCAG TTGTGTGGCC AATATGGGCA GCCTGTCCTT 
GCTGTTAAAG TTCTCTTTGA AATGAGAAAG GTCGGCGTGC AGCCAAATGC TATAACATAT 
GGCTATTATA ATAAGGCTGT CCTGGAAAGC GTATGGCCCT CTGGGAATAG AGGGGGTTAC 
TTTCTGTGGA TAAAGGTGAG GAATGTGGTG CTAGCAGTAG CCCAGTTCAA ACAGGCTTTG 
ATAAAGCAAC ACGCTTCAGC TCATCAAACT CCTGCTTCAG ATTGCAGTGA TTTGGACGCA 
GTCAGCCATG GCAGTTTGGA TAGTTCTGTT GACATGAACC CTACAGAACA GCCTTCATTC 
AGTACAGATG TAACATGTGC TGATCCAGCT GACGACCGAT CTAGTACAGG AGGCCAGTCA 
GACCTTGGCT ACAGTTCATT ATCAAAAGAA GAAGTAAGAA GAGGAGCCGT GCATGACATA 
CAGGAGGAGA AGGAAGACTC CAGAGATAGT GACTCCAGTT CGTCATCTGA TTCAGAAAGT 
TCCAAGAGCA GCAGAGACTG TGTGCCACAA GGAACTGGCA GTCAGGGTCA ACAAACATTG 
GCAAGCACAT CTAGTATAGT CCGGAAAGAA GCAAAATCAG ACAGTGCAAG CAGTGACAAC 
TCAGCAGGTC TACTGTTCAC ACCATCCTTT AGTGAGAATT GTGAAAGTGA AGTGTATCAG 
TGCTGGAGTC TACATAAAAG ACACAAGAGT CCTGTGGAGT CAGATCTGAA AAAACAGATG 
GAATGGGAAA AGAGGAACAG GCATCTAAGC AGTGGCAATG TTGAGGAATT ATTTATCAGT 
AAGTCTGATG GACAGAATAA CACTGAAAAC ATGGTGGACA AACTTGGAGC AGATGCAAAA 
ATACTTAGCA CTGTATATAA GCAACACATG GCACGGCCCA AGACCCTGGA CTTAAATAAA 
ATGAATCCCA GAAAGTCAAA GGAGGTTGTT GAAAAAGAAT CTAGTGATGA TGAGTCTCCC 
ATAGGAGGAG CAGATGACAA AGTGGACTCC CCTGTTATTT TTGACCTTGA AGATCTAGAT 
AATGATTCTG ATGAACTTAA ATCAGCAAAA CCATGTGTCC GGAAGGCCAA ACTTGCTCAA 
AGAAGTATCA GCATGCCCTT TTCATCTTCC ATGTTTCTGG CTAAGCCAGC TGAAAAAACA 
GACGTGAAGG CTGGATTAGA CCCTTTGTCA TTGCTGGCAG CTGAAACAGA ACAGGCAGAA 
GTAGAACAGG AGGAGGAAGA TGACAAAAGC TTACTAACAC CATCTGCTAA AAGGGACCTT 
GCAGAGGAAA TAGAAATGTA CATGAACAAT ATAAGCAGCC CCCTTGCAAG TCGAACCCCT 
AGCATGGACC TTGACAGATC CAGTTCTGAA CTAAATAAAA CATGTAAAGC TGTAGCACTC 
AACAGGCGCT TGAGTGTTCC TGCTAGATCC CCACAAGAAC CAAGACTAAC CAGATCAATT 
ACTGCCCACA GTAAACGCGA CAAAAGACAA AGAAGCAGGC TCTGGTCTTC TCCTGCTGTT 
TCCGAGAACA ATCCTCAGAA TGAGCAAAGC CCAGAATCTG CCCTTGCTTT AATGTCCCCA 
ACATCATTTA ACATAGATAC ACTTCTCACG CCAACCTTTG ATGTTCTTAG GAGCAACATG 
TTCTCAGCTG GCAAAGGAGT AGCCAAGAAG GCCAGCAAAT GGTATTCAAA GATCGCAAAT 
TATGCGGGGC CTGCAAAGGA TCAGAATTCT GACCGAGCCA GTCTGTCTTC AGGAGGAGCA 
GGGGATACTG AATCCTACTC TTTAAATGAT GATGATTTTG ATGGCAGTGC TTCTCTGCAT 
GAGACTGATG TCTTTCCTGG AACCAATACC ATTGGTGTAA GCAGATGCAG CTTGGAGAGT 
AGCACATCTC AAGACAGTAA CTCTAAACAA CTTTACAATA ATGGTCTGCC ATCAACAGCA 
TCTCCAAACT ACAAGAGTGA GATTCTTTCA TCCTGCAATT CCAGTAGCAC TAGTGTCTTC 
CAGAACTATG CTATGGAGGT TCTGATATCT AGCTGTTCAC GTTGCTTGAC CTGTGACGGT 
CTTGTTCATG ATGAGGAGAT TATGGCAGGC TGGACGGCAG ATGACTCAAA CCTTAACACC 
ACATGTCCTT TTTGCGGGAA CTCATTTCTT CCATCTTTAA ACGTTGAAAT TAGGGACCTG 
CGACGCCCTG GAAGTAAAAC CAAACAAGAG AGAACTCGGT CTGTAACTGT TGAGATCCCA 
GTGGGGAAGA GAACCAGAGA TCTCAATGGG TCCTTATGCC CGAATCAGCC CATTGCACGA 
AGTATCAGCA CATTTGGTCC CTTAGATGAA CAGCCATCTC ATGACCAGAG CCATGTTATT 
GCCACTGGCA GCCTCCCAAC TTGCTTCCAG GCACCTCCTG AGTCAGGATT GGAATGGAAG 
ATGTCAAATC CAGAGCCAAT CACAGTCCCT TACCTTAGCC CGCTGGTTGT CTGGAAGGAG 
CTGGAGAGCC TGCTAGAAAA TGAGGGTGAC CAATCGATTA CAGTTTCTGG ATTTGTGGAC 
CATCATCCGA TAGTATTCTG GAATTTGGTG TGGTATTTCA GGAGGCTGGA TCTCCCAAGT 
AACCTCCCGG GATTAATTCT GTCTTCGGAA CAATGCAATA AAAATTCAAA GGTCTCCAGG 
AGCTGTATGT CTGCAGACAG CAAACATGTC CTTATTCAAG TGCTGTGGGA CAATCTGAAA 
TTGCACCAGG ATCCCCGGCA GCCTCTCTAC ATCCTCTGGA ACACTATTAG TCAAAACCGC 
ACTGTATTGT TTGATACACA GGAACATGCG CTTGCTGTGC CACAGATCAA AGAGGAGGAA 
TTGTTTGATC AGCAGTTTCT CAGAGGGATA GTAAGAAGCA TAAAGAACAA CAATGTTTAT 
GGTCCTGTGA GCCAGATATT GGACTGTATT AAACAGGTCC CTGGAAGAAC GAGGCAGAGG 
AGTCTGTACA GGGAAATTCT TTTTCTTTCT CTTGTTGCCT TGGGAAGAGA GAATATCGAT 
ATTGATGTAT TTGATCGGGA ATATAAAACT GCATACGAGC GCCTCACCCC TGAGCAGCTG 
AACTGCACAC AGATTTGTGA TCGGCCTCCC AGCATTGGAG TGATGGAGTG CAGGAAGACT 
TTCGGACAGC CATTCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4a ( XM_018092501.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947990.1 Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa43410
Clone ID Related Accession (Same CDS sequence)	XM_018092501.1
Accession Version	XM_018092501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	C-myc promoter-binding protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCTGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGGCGG ATGTGGATCG ACCAGGAGAG 
ACACGTCTTA TTGAACTGGA TGAGTCTCAC AGAAGTGAGC ATACAGTGTT CATCACACCA 
CCAGAGATTC CCCACTTACC AAATGGCGAG GAGCCCCCTA AGAACTATAG CTACAATGGG 
TTCCCTGTCC TTATCTTGGA TCTCTTTGAC ACGCCAGATG GACTCATGAA AAGTCACAAA 
CTTAGTACAA AAGTCACCAC ACCCTGCAGT CCTTCTCCAA TGTTTAGAAG AACAAAACAG 
GAAATCCGGT CAGCACATAA AATAGCAAAG CAGTACTCCT CCATCCCCCA GATGTGGTCC 
AGGTGTCTAC TGAGACATTG CTATGGACTT TGGTTTATCT GTCTGCCCGC ATATGTGAAA 
TTCTGCCATT CCAAAGTGAG AGCACTGCGC ACAGCCTACG ATGTGTTGCG AAAAATGCAC 
ATGAAAAAGA TTGACTCTCC TGATGAGGTT TGCTATAGGG TTCTAATGCA GTTGTGTGGC 
CAATATGGGC AGCCTGTCCT TGCTGTTAAA GTTCTCTTTG AAATGAGAAA GGTCGGCGTG 
CAGCCAAATG CTATAACATA TGGCTATTAT AATAAGGCTG TCCTGGAAAG CGTATGGCCC 
TCTGGGAATA GAGGGGGTTA CTTTCTGTGG ATAAAGGTGA GGAATGTGGT GCTAGCAGTA 
GCCCAGTTCA AACAGGCTTT GATAAAGCAA CACGCTTCAG CTCATCAAAC TCCTGCTTCA 
GATTGCAGTG ATTTGGACGC AGTCAGCCAT GGCAGTTTGG ATAGTTCTGT TGACATGAAC 
CCTACAGAAC AGCCTTCATT CAGTACAGAT GTAACATGTG CTGATCCAGC TGACGACCGA 
TCTAGTACAG GAGGCCAGTC AGACCTTGGC TACAGTTCAT TATCAAAAGA AGAAGTAAGA 
AGAGGAGCCG TGCATGACAT ACAGGAGGAG AAGGAAGACT CCAGAGATAG TGACTCCAGT 
TCGTCATCTG ATTCAGAAAG TTCCAAGAGC AGCAGAGACT GTGTGCCACA AGGAACTGGC 
AGTCAGGGTC AACAAACATT GGCAAGCACA TCTAGTATAG TCCGGAAAGA AGCAAAATCA 
GACAGTGCAA GCAGTGACAA CTCAGCAGGT CTACTGTTCA CACCATCCTT TAGTGAGAAT 
TGTGAAAGTG AAGTGTATCA GTGCTGGAGT CTACATAAAA GACACAAGAG TCCTGTGGAG 
TCAGATCTGA AAAAACAGAT GGAATGGGAA AAGAGGAACA GGCATCTAAG CAGTGGCAAT 
GTTGAGGAAT TATTTATCAG TAAGTCTGAT GGACAGAATA ACACTGAAAA CATGGTGGAC 
AAACTTGGAG CAGATGCAAA AATACTTAGC ACTGTATATA AGCAACACAT GGCACGGCCC 
AAGACCCTGG ACTTAAATAA AATGAATCCC AGAAAGTCAA AGGAGGTTGT TGAAAAAGAA 
TCTAGTGATG ATGAGTCTCC CATAGGAGGA GCAGATGACA AAGTGGACTC CCCTGTTATT 
TTTGACCTTG AAGATCTAGA TAATGATTCT GATGAACTTA AATCAGCAAA ACCATGTGTC 
CGGAAGGCCA AACTTGCTCA AAGAAGTATC AGCATGCCCT TTTCATCTTC CATGTTTCTG 
GCTAAGCCAG CTGAAAAAAC AGACGTGAAG GCTGGATTAG ACCCTTTGTC ATTGCTGGCA 
GCTGAAACAG AACAGGCAGA AGTAGAACAG GAGGAGGAAG ATGACAAAAG CTTACTAACA 
CCATCTGCTA AAAGGGACCT TGCAGAGGAA ATAGAAATGT ACATGAACAA TATAAGCAGC 
CCCCTTGCAA GTCGAACCCC TAGCATGGAC CTTGACAGAT CCAGTTCTGA ACTAAATAAA 
ACATGTAAAG CTGTAGCACT CAACAGGCGC TTGAGTGTTC CTGCTAGATC CCCACAAGAA 
CCAAGACTAA CCAGATCAAT TACTGCCCAC AGTAAACGCG ACAAAAGACA AAGAAGCAGG 
CTCTGGTCTT CTCCTGCTGT TTCCGAGAAC AATCCTCAGA ATGAGCAAAG CCCAGAATCT 
GCCCTTGCTT TAATGTCCCC AACATCATTT AACATAGATA CACTTCTCAC GCCAACCTTT 
GATGTTCTTA GGAGCAACAT GTTCTCAGCT GGCAAAGGAG TAGCCAAGAA GGCCAGCAAA 
TGGTATTCAA AGATCGCAAA TTATGCGGGG CCTGCAAAGG ATCAGAATTC TGACCGAGCC 
AGTCTGTCTT CAGGAGGAGC AGGGGATACT GAATCCTACT CTTTAAATGA TGATGATTTT 
GATGGCAGTG CTTCTCTGCA TGAGACTGAT GTCTTTCCTG GAACCAATAC CATTGGTGTA 
AGCAGATGCA GCTTGGAGAG TAGCACATCT CAAGACAGTA ACTCTAAACA ACTTTACAAT 
AATGGTCTGC CATCAACAGC ATCTCCAAAC TACAAGAGTG AGATTCTTTC ATCCTGCAAT 
TCCAGTAGCA CTAGTGTCTT CCAGAACTAT GCTATGGAGG TTCTGATATC TAGCTGTTCA 
CGTTGCTTGA CCTGTGACGG TCTTGTTCAT GATGAGGAGA TTATGGCAGG CTGGACGGCA 
GATGACTCAA ACCTTAACAC CACATGTCCT TTTTGCGGGA ACTCATTTCT TCCATCTTTA 
AACGTTGAAA TTAGGGACCT GCGACGCCCT GGAAGGTACT TTCTGAAGGC CAGCCCATCT 
GCAGAAAGTA TGCCGCATTC TTCAGTATCC TCACAAAGAC CTAACTCAGA TATTCTGACT 
CCGGCTTCTC CCTGTGCTTC CACCAAAGTC AAGGATTTTA GTTTGTGCAG TAAAACCAAA 
CAAGAGAGAA CTCGGTCTGT AACTGTTGAG ATCCCAGTGG GGAAGAGAAC CAGAGATCTC 
AATGGGTCCT TATGCCCGAA TCAGCCCATT GCACGAAGTA TCAGCACATT TGGTCCCTTA 
GATGAACAGC CATCTCATGA CCAGAGCCAT GTTATTGCCA CTGGCAGCCT CCCAACTTGC 
TTCCAGGCAC CTCCTGAGTC AGGATTGGAA TGGAAGATGT CAAATCCAGA GCCAATCACA 
GTCCCTTACC TTAGCCCGCT GGTTGTCTGG AAGGAGCTGG AGAGCCTGCT AGAAAATGAG 
GGTGACCAAT CGATTACAGT TTCTGGATTT GTGGACCATC ATCCGATAGT ATTCTGGAAT 
TTGGTGTGGT ATTTCAGGAG GCTGGATCTC CCAAGTAACC TCCCGGGATT AATTCTGTCT 
TCGGAACAAT GCAATAAAAA TTCAAAGGTC TCCAGGAGCT GTATGTCTGC AGACAGCAAA 
CATGTCCTTA TTCAAGTGCT GTGGGACAAT CTGAAATTGC ACCAGGATCC CCGGCAGCCT 
CTCTACATCC TCTGGAACAC TATTACACAG GAACATGCGC TTGCTGTGCC ACAGATCAAA 
GAGGAGGAAT TGTTTGATCA GCAGTTTCTC AGAGGGATAG TAAGAAGCAT AAAGAACAAC 
AATGTTTATG GTCCTGTGAG CCAGATATTG GACTGTATTA AACAGGTCCC TGGAAGAACG 
AGGCAGAGGA GTCTGTACAG GGAAATTCTT TTTCTTTCTC TTGTTGCCTT GGGAAGAGAG 
AATATCGATA TTGATGTATT TGATCGGGAA TATAAAACTG CATACGAGCG CCTCACCCCT 
GAGCAGCTGA ACTGCACACA GATTTGTGAT CGGCCTCCCA GCATTGGAGT GATGGAGTGC 
AGGAAGACTT TCGGACAGCC ATTCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947990.1
CDS	27..5756
Translation	MMEDKGPRVADYFVVTGLTDSSKPLEEEIHFNDACHKTAKPKEPITDVTVIIKSLGEEVPQGYVCIDLTPTGLSADLNNGSIRGPQIYLCYRRGRDKPPLTDLGVLYEWKERLKQGCKIIQATPYGRPANISGSASSQRVFITYRRAAESMTQNSLAVTDICIIIPSKGETPPHTFCKVEKNLNSSVWGSAVYLCYKKSVAKTNAVSYKAGLICRYPEEDYESFPLPESVPLFCLPMGATIECWPSDSNYPLPVFSTFVLTGASAEKVYGAAIQFYEPYAQDQLTEKQQVHLGLLSSAEGQMDSSKSIHVNKCICLLSHWPFFDAFKKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDTLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQIEGKRNVTWKILPKKACKNLLNTLCNLYQQLAELQHRPREEAFMELAMNDYDFNYGKRMHQLDMEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFELQGFLKSRDRSHHKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVEKADVDRPGETRLIELDESHRSEHTVFITPPEIPHLPNGEEPPKNYSYNGFPVLILDLFDTPDGLMKSHKLSTKVTTPCSPSPMFRRTKQEIRSAHKIAKQYSSIPQMWSRCLLRHCYGLWFICLPAYVKFCHSKVRALRTAYDVLRKMHMKKIDSPDEVCYRVLMQLCGQYGQPVLAVKVLFEMRKVGVQPNAITYGYYNKAVLESVWPSGNRGGYFLWIKVRNVVLAVAQFKQALIKQHASAHQTPASDCSDLDAVSHGSLDSSVDMNPTEQPSFSTDVTCADPADDRSSTGGQSDLGYSSLSKEEVRRGAVHDIQEEKEDSRDSDSSSSSDSESSKSSRDCVPQGTGSQGQQTLASTSSIVRKEAKSDSASSDNSAGLLFTPSFSENCESEVYQCWSLHKRHKSPVESDLKKQMEWEKRNRHLSSGNVEELFISKSDGQNNTENMVDKLGADAKILSTVYKQHMARPKTLDLNKMNPRKSKEVVEKESSDDESPIGGADDKVDSPVIFDLEDLDNDSDELKSAKPCVRKAKLAQRSISMPFSSSMFLAKPAEKTDVKAGLDPLSLLAAETEQAEVEQEEEDDKSLLTPSAKRDLAEEIEMYMNNISSPLASRTPSMDLDRSSSELNKTCKAVALNRRLSVPARSPQEPRLTRSITAHSKRDKRQRSRLWSSPAVSENNPQNEQSPESALALMSPTSFNIDTLLTPTFDVLRSNMFSAGKGVAKKASKWYSKIANYAGPAKDQNSDRASLSSGGAGDTESYSLNDDDFDGSASLHETDVFPGTNTIGVSRCSLESSTSQDSNSKQLYNNGLPSTASPNYKSEILSSCNSSSTSVFQNYAMEVLISSCSRCLTCDGLVHDEEIMAGWTADDSNLNTTCPFCGNSFLPSLNVEIRDLRRPGRYFLKASPSAESMPHSSVSSQRPNSDILTPASPCASTKVKDFSLCSKTKQERTRSVTVEIPVGKRTRDLNGSLCPNQPIARSISTFGPLDEQPSHDQSHVIATGSLPTCFQAPPESGLEWKMSNPEPITVPYLSPLVVWKELESLLENEGDQSITVSGFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEQCNKNSKVSRSCMSADSKHVLIQVLWDNLKLHQDPRQPLYILWNTITQEHALAVPQIKEEELFDQQFLRGIVRSIKNNNVYGPVSQILDCIKQVPGRTRQRSLYREILFLSLVALGRENIDIDVFDREYKTAYERLTPEQLNCTQICDRPPSIGVMECRKTFGQPFL

Target ORF information:

RefSeq Version	XM_018092501.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN/MADD domain containing 4A (dennd4a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092501.1

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCTGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGGCGG ATGTGGATCG ACCAGGAGAG 
ACACGTCTTA TTGAACTGGA TGAGTCTCAC AGAAGTGAGC ATACAGTGTT CATCACACCA 
CCAGAGATTC CCCACTTACC AAATGGCGAG GAGCCCCCTA AGAACTATAG CTACAATGGG 
TTCCCTGTCC TTATCTTGGA TCTCTTTGAC ACGCCAGATG GACTCATGAA AAGTCACAAA 
CTTAGTACAA AAGTCACCAC ACCCTGCAGT CCTTCTCCAA TGTTTAGAAG AACAAAACAG 
GAAATCCGGT CAGCACATAA AATAGCAAAG CAGTACTCCT CCATCCCCCA GATGTGGTCC 
AGGTGTCTAC TGAGACATTG CTATGGACTT TGGTTTATCT GTCTGCCCGC ATATGTGAAA 
TTCTGCCATT CCAAAGTGAG AGCACTGCGC ACAGCCTACG ATGTGTTGCG AAAAATGCAC 
ATGAAAAAGA TTGACTCTCC TGATGAGGTT TGCTATAGGG TTCTAATGCA GTTGTGTGGC 
CAATATGGGC AGCCTGTCCT TGCTGTTAAA GTTCTCTTTG AAATGAGAAA GGTCGGCGTG 
CAGCCAAATG CTATAACATA TGGCTATTAT AATAAGGCTG TCCTGGAAAG CGTATGGCCC 
TCTGGGAATA GAGGGGGTTA CTTTCTGTGG ATAAAGGTGA GGAATGTGGT GCTAGCAGTA 
GCCCAGTTCA AACAGGCTTT GATAAAGCAA CACGCTTCAG CTCATCAAAC TCCTGCTTCA 
GATTGCAGTG ATTTGGACGC AGTCAGCCAT GGCAGTTTGG ATAGTTCTGT TGACATGAAC 
CCTACAGAAC AGCCTTCATT CAGTACAGAT GTAACATGTG CTGATCCAGC TGACGACCGA 
TCTAGTACAG GAGGCCAGTC AGACCTTGGC TACAGTTCAT TATCAAAAGA AGAAGTAAGA 
AGAGGAGCCG TGCATGACAT ACAGGAGGAG AAGGAAGACT CCAGAGATAG TGACTCCAGT 
TCGTCATCTG ATTCAGAAAG TTCCAAGAGC AGCAGAGACT GTGTGCCACA AGGAACTGGC 
AGTCAGGGTC AACAAACATT GGCAAGCACA TCTAGTATAG TCCGGAAAGA AGCAAAATCA 
GACAGTGCAA GCAGTGACAA CTCAGCAGGT CTACTGTTCA CACCATCCTT TAGTGAGAAT 
TGTGAAAGTG AAGTGTATCA GTGCTGGAGT CTACATAAAA GACACAAGAG TCCTGTGGAG 
TCAGATCTGA AAAAACAGAT GGAATGGGAA AAGAGGAACA GGCATCTAAG CAGTGGCAAT 
GTTGAGGAAT TATTTATCAG TAAGTCTGAT GGACAGAATA ACACTGAAAA CATGGTGGAC 
AAACTTGGAG CAGATGCAAA AATACTTAGC ACTGTATATA AGCAACACAT GGCACGGCCC 
AAGACCCTGG ACTTAAATAA AATGAATCCC AGAAAGTCAA AGGAGGTTGT TGAAAAAGAA 
TCTAGTGATG ATGAGTCTCC CATAGGAGGA GCAGATGACA AAGTGGACTC CCCTGTTATT 
TTTGACCTTG AAGATCTAGA TAATGATTCT GATGAACTTA AATCAGCAAA ACCATGTGTC 
CGGAAGGCCA AACTTGCTCA AAGAAGTATC AGCATGCCCT TTTCATCTTC CATGTTTCTG 
GCTAAGCCAG CTGAAAAAAC AGACGTGAAG GCTGGATTAG ACCCTTTGTC ATTGCTGGCA 
GCTGAAACAG AACAGGCAGA AGTAGAACAG GAGGAGGAAG ATGACAAAAG CTTACTAACA 
CCATCTGCTA AAAGGGACCT TGCAGAGGAA ATAGAAATGT ACATGAACAA TATAAGCAGC 
CCCCTTGCAA GTCGAACCCC TAGCATGGAC CTTGACAGAT CCAGTTCTGA ACTAAATAAA 
ACATGTAAAG CTGTAGCACT CAACAGGCGC TTGAGTGTTC CTGCTAGATC CCCACAAGAA 
CCAAGACTAA CCAGATCAAT TACTGCCCAC AGTAAACGCG ACAAAAGACA AAGAAGCAGG 
CTCTGGTCTT CTCCTGCTGT TTCCGAGAAC AATCCTCAGA ATGAGCAAAG CCCAGAATCT 
GCCCTTGCTT TAATGTCCCC AACATCATTT AACATAGATA CACTTCTCAC GCCAACCTTT 
GATGTTCTTA GGAGCAACAT GTTCTCAGCT GGCAAAGGAG TAGCCAAGAA GGCCAGCAAA 
TGGTATTCAA AGATCGCAAA TTATGCGGGG CCTGCAAAGG ATCAGAATTC TGACCGAGCC 
AGTCTGTCTT CAGGAGGAGC AGGGGATACT GAATCCTACT CTTTAAATGA TGATGATTTT 
GATGGCAGTG CTTCTCTGCA TGAGACTGAT GTCTTTCCTG GAACCAATAC CATTGGTGTA 
AGCAGATGCA GCTTGGAGAG TAGCACATCT CAAGACAGTA ACTCTAAACA ACTTTACAAT 
AATGGTCTGC CATCAACAGC ATCTCCAAAC TACAAGAGTG AGATTCTTTC ATCCTGCAAT 
TCCAGTAGCA CTAGTGTCTT CCAGAACTAT GCTATGGAGG TTCTGATATC TAGCTGTTCA 
CGTTGCTTGA CCTGTGACGG TCTTGTTCAT GATGAGGAGA TTATGGCAGG CTGGACGGCA 
GATGACTCAA ACCTTAACAC CACATGTCCT TTTTGCGGGA ACTCATTTCT TCCATCTTTA 
AACGTTGAAA TTAGGGACCT GCGACGCCCT GGAAGGTACT TTCTGAAGGC CAGCCCATCT 
GCAGAAAGTA TGCCGCATTC TTCAGTATCC TCACAAAGAC CTAACTCAGA TATTCTGACT 
CCGGCTTCTC CCTGTGCTTC CACCAAAGTC AAGGATTTTA GTTTGTGCAG TAAAACCAAA 
CAAGAGAGAA CTCGGTCTGT AACTGTTGAG ATCCCAGTGG GGAAGAGAAC CAGAGATCTC 
AATGGGTCCT TATGCCCGAA TCAGCCCATT GCACGAAGTA TCAGCACATT TGGTCCCTTA 
GATGAACAGC CATCTCATGA CCAGAGCCAT GTTATTGCCA CTGGCAGCCT CCCAACTTGC 
TTCCAGGCAC CTCCTGAGTC AGGATTGGAA TGGAAGATGT CAAATCCAGA GCCAATCACA 
GTCCCTTACC TTAGCCCGCT GGTTGTCTGG AAGGAGCTGG AGAGCCTGCT AGAAAATGAG 
GGTGACCAAT CGATTACAGT TTCTGGATTT GTGGACCATC ATCCGATAGT ATTCTGGAAT 
TTGGTGTGGT ATTTCAGGAG GCTGGATCTC CCAAGTAACC TCCCGGGATT AATTCTGTCT 
TCGGAACAAT GCAATAAAAA TTCAAAGGTC TCCAGGAGCT GTATGTCTGC AGACAGCAAA 
CATGTCCTTA TTCAAGTGCT GTGGGACAAT CTGAAATTGC ACCAGGATCC CCGGCAGCCT 
CTCTACATCC TCTGGAACAC TATTACACAG GAACATGCGC TTGCTGTGCC ACAGATCAAA 
GAGGAGGAAT TGTTTGATCA GCAGTTTCTC AGAGGGATAG TAAGAAGCAT AAAGAACAAC 
AATGTTTATG GTCCTGTGAG CCAGATATTG GACTGTATTA AACAGGTCCC TGGAAGAACG 
AGGCAGAGGA GTCTGTACAG GGAAATTCTT TTTCTTTCTC TTGTTGCCTT GGGAAGAGAG 
AATATCGATA TTGATGTATT TGATCGGGAA TATAAAACTG CATACGAGCG CCTCACCCCT 
GAGCAGCTGA ACTGCACACA GATTTGTGAT CGGCCTCCCA GCATTGGAGT GATGGAGTGC 
AGGAAGACTT TCGGACAGCC ATTCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4a ( XM_031899250.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755110.1 Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X1, mRNA.

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CloneID	OXa55245
Clone ID Related Accession (Same CDS sequence)	XM_031899250.1
Accession Version	XM_031899250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACTT TTGAGGAAGA GCAAGTAAGC AGTTGTCCTA CTCCAAAACG GAAGATGATG 
GAAGACAAAG GGCCCCGTGT GGCTGACTAC TTTGTAGTTA CAGGACTGAC AGATTCATCA 
AAACCACTTG AGGAGGAGAT CCACTTTAAT GATGCCTGTC ATAAAACAGC TAAACCAAAG 
GAACCCATTA CAGATGTGAC TGTGATAATC AAATCCTTAG GGGAGGAAGT GCCACAGGGA 
TATGTATGCA TAGACCTTAC CCCAACTGGA CTATCAGCCG ATCTCAATAA TGGAAGTATT 
CGAGGGCCAC AGATATATCT TTGTTACAGG AGAGGAAGAG ACAAACCACC TCTAACAGAT 
CTTGGGGTAT TGTACGAATG GAAGGAAAGA TTAAAACAGG GCTGCAAGAT TATTCAGGCT 
ACTCCATATG GCCGTCCAGC TAATATAAGC GGTAGTGCAT CCTCACAGAG AGTATTTATC 
ACATACCGCA GAGCTGCTGA GAGCATGACC CAGAATTCCT TGGCTGTAAC TGATATCTGC 
ATCATTATAC CAAGCAAAGG AGAGACTCCA CCACACACAT TTTGCAAAGT GGAAAAGAAT 
CTGAACAGCA GCGTGTGGGG CTCTGCTGTG TACCTGTGCT ACAAGAAATC AGTTGCCAAA 
ACAAATGCAG TATCCTATAA AGCTGGGTTG ATATGCAGGT ATCCAGAGGA GGATTATGAA 
TCATTTCCTC TGCCGGAATC AGTCCCACTA TTTTGTCTAC CCATGGGGGC AACAATTGAA 
TGTTGGCCAT CTGACAGTAA TTATCCACTC CCTGTTTTTT CCACTTTTGT TTTAACTGGA 
GCATCAGCTG AAAAGGTGTA TGGAGCTGCT ATTCAGTTTT ATGAGCCATA TGCTCAAGAT 
CAGCTGACTG AAAAGCAACA AGTACATTTA GGCCTCTTGA GCAGTGCAGA GGGTCAGATG 
GACTCTTCTA AAAGTATCCA TGTCAACAAG TGCATCTGCC TGCTGTCCCA CTGGCCTTTT 
TTTGATGCTT TCAAGAAATT TCTCACTTTC CTTTACCGCT ATTCAATATC GGGTCCTCAT 
GTGCTGCCAA TAGAAAAACA TATCTCCCAC TTCATGCACA AAGTCCCATT CCCATCTCCA 
CAGAGACCAC GCATTCTAGT TCAGCTTTCC CCGCATGATA CTCTGATCCT AAGCCAGCCT 
GTGTCCTCAC CACTTCCACT GAGTGGTGGA AAGTTTTCAA CGTTGCTGCA GAATTTGGGA 
CCTGAAAATG CTGTGACTCT GCTTGTATTT GCTGTCACGG AGCATAAGAT TCTGATCCAT 
TCGTTGCGCC CCTCGGTTCT CACAAGCGTC ACAGAAGCTT TAGTGTCTAT GATCTTCCCT 
TTCCATTGGC CGTGTCCTTA CATTCCACTT TGCCCTTTGG CCCTGGCAGA CGTACTAAGC 
GCCCCATGCC CCTTCATCGT GGGAATTGAT TCTCGCTACT TTGATCTGTA CGACCCCCCT 
CCGGATGTAA GCTGTGTTGA CCTAGATACC AACACCATCT CTCAAATTGA AGGCAAAAGA 
AATGTAACAT GGAAAATCCT ACCGAAGAAA GCTTGTAAGA ACCTGCTCAA CACTCTTTGT 
AACTTGTACC AGCAACTGGC AGAGTTGCAA CACAGACCAC GGGAAGAAGC TTTTATGGAA 
CTTGCAATGA ATGACTATGA CTTTAACTAT GGAAAAAGAA TGCATCAGCT GGACATGGAG 
ATTCAGGAAG CATTCCTGTG CTTTATGGCC TCTATTTTAA AAGGATACAG ATCGTATCTT 
CGGCCAATTA CCCAAGCACC ATCAGAGACT GCAACAGATG CCAGCTCTCT CTTCGAACTG 
CAAGGTTTCT TAAAAAGTCG TGATCGCTCA CACCACAAGT TTTACAACAT GATGACCAAG 
ACTCAGATGT TCATCCGCTT TATTGAGGAG TGTTCCTTCG TCAGCGATAA GGATGCTAGC 
CTGGCTTTCT TTGATGACTG TGTTGAGAAG CTTTTCTCAG AGAGGAATAA TGCAGATAAA 
GGTGGCAAGG CGGATGTGGA TCGACCAGGA GAGACACGTC TTATTGAACT GGATGAGTCT 
CACAGAAGTG AGCATACAGT GTTCATCACA CCACCAGAGA TTCCCCACTT ACCAAATGGC 
GAGGAGCCCC CTAAGAACTA TAGCTACAAT GGGTTCCCTG TCCTTATCTT GGATCTCTTT 
GACACGCCAG ATGGACTCAT GAAAAGTCAC AAACTTAGTA CAAAAGTCAC CACACCCTGC 
AGTCCTTCTC CAATGTTTAG AAGAACAAAA CAGGAAATCC GGTCAGCACA TAAAATAGCA 
AAGCAGTACT CCTCCATCCC CCAGATGTGG TCCAGGTGTC TACTGAGACA TTGCTATGGA 
CTTTGGTTTA TCTGTCTGCC CGCATATGTG AAATTCTGCC ATTCCAAAGT GAGAGCACTG 
CGCACAGCCT ACGATGTGTT GCGAAAAATG CACATGAAAA AGATTGACTC TCCTGATGAG 
GTTTGCTATA GGGTTCTAAT GCAGTTGTGT GGCCAATATG GGCAGCCTGT CCTTGCTGTT 
AAAGTTCTCT TTGAAATGAG AAAGGTCGGC GTGCAGCCAA ATGCTATAAC ATATGGCTAT 
TATAATAAGG CTGTCCTGGA AAGCGTATGG CCCTCTGGGA ATAGAGGGGG TTACTTTCTG 
TGGATAAAGG TGAGGAATGT GGTGCTAGCA GTAGCCCAGT TCAAACAGGC TTTGATAAAG 
CAACATGCTT CAGCTCATCA AACTCCTGCT TCAGATTGCA GTGATTTGGA CGCAGTCAGC 
CATGGCAGTT TGGATAGTTC TGTTGACATG AACCCTACAG AACAGCCTTC ATTCAGTACA 
GATGTAACAT GTGCTGATCC AGCTGACGAC CGATCTAGTA CAGGAGGCCA GTCAGACCTT 
GGCTACAGTT CATTATCAAA AGAAGAAGTA AGAAGAGGAG CCGTGCATGA CATACAGGAG 
GAGAAGGAAG ACTCCAGAGA TAGTGACTCC AGTTCGTCAT CTGATTCAGA AAGTTCCAAG 
AGCAGCAGAG ACTGTGTGCC ACAAGGAACT GGCAGTCAGG GTCAACAAAC ATTGGCAAGC 
ACATCTAGTA TAGTCCGGAA AGAAGCAAAA TCAGACAGTG CAAGCAGTGA CAACTCAGCA 
GGTCTACTGT TCACACCATC CTTTAGTGAG AATTGTGAAA GTGAAGTGTA TCAGTGCTGG 
AGTCTACATA AAAGACACAA GAGTCCTGTG GAGTCAGATC TGAAAAAACA GATGGAATGG 
GAAAAGAGGA ACAGGCATCT AAGCAGTGGC AATGTTGAGG AATTATTTAT CAGTAAGTCT 
GATGGACAGA ATAACACTGA AAACATGGTG GACAAACTTG GAGCAGATGC AAAAATACTT 
AGCACTGTAT ATAAGCAACA CATGGCACGG CCCAAGACCC TGGACTTAAA TAAAATGAAT 
CCCAGAAAGT CAAAGGAGGT TGTTGAAAAA GAATCTAGTG ATGATGAGTC TCCCATAGGA 
GGAGCAGATG ACAAAGTGGA CTCCCCTGTT ATTTTTGACC TTGAAGATCT AGATAATGAT 
TCTGATGAAC TTAAATCAGC AAAACCATGT GTCCGGAAGG CCAAACTTGC TCAAAGAAGT 
ATCAGCATGC CCTTTTCATC TTCCATGTTT CTGGCTAAGC CAGCTGAAAA AACAGACGTG 
AAGGCTGGAT TAGACCCTTT GTCATTGCTG GCAGCTGAAA CAGAACAGGC AGAAGTAGAA 
CAGGAGGAGG AAGATGACAA AAGCTTACTA ACACCATCTG CTAAAAGGGA CCTTGCAGAG 
GAAATAGAAA TGTACATGAA CAATATAAGC AGCCCCCTTG CAAGTCGAAC CCCTAGCATG 
GACCTTGACA GATCCAGTTC TGAACTAAAT AAAACATGTA AAGCTGTAGC ACTCAACAGG 
CGCTTGAGTG TTCCTGCTAG ATCCCCACAA GAACCAAGAC TAACCAGATC AATTACTGCC 
CACAGTAAAC GCGACAAAAG ACAAAGAAGC AGGCTCTGGT CTTCTCCTGC TGTTTCCGAG 
AACAATCCTC AGAATGAGCA AAGCCCAGAA TCTGCCCTTG CTTTAATGTC CCCAACATCA 
TTTAACATAG ATACACTTCT CACGCCAACC TTTGATGTTC TTAGGAGCAA CATGTTCTCA 
GCTGGCAAAG GAGTAGCCAA GAAGGCCAGC AAATGGTATT CAAAGATCGC AAATTATGCG 
GGGCCTGCAA AGGATCAGAA TTCTGACCGA GCCAGTCTGT CTTCAGGAGG AGCAGGGGAT 
ACTGAATCCT ACTCTTTAAA TGATGATGAT TTTGATGGCA GTGCTTCTCT GCATGAGACT 
GATGTCTTTC CTGGAACCAA TACCATTGGT GTAAGCAGAT GCAGCTTGGA GAGTAGCACA 
TCTCAAGACA GTAACTCTAA ACAACTTTAC AATAATGGTC TGCCATCAAC AGCATCTCCA 
AACTACAAGA GTGAGATTCT TTCATCCTGC AATTCCAGTA GCACTAGTGT CTTCCAGAAC 
TATGCTATGG AGGTTCTGAT ATCTAGCTGT TCACGTTGCT TGACCTGTGA CGGTCTTGTT 
CATGATGAGG AGATTATGGC AGGCTGGACG GCAGATGACT CAAACCTTAA CACCACATGT 
CCTTTTTGCG GGAACTCATT TCTTCCATCT TTAAACGTTG AAATTAGGGA CCTGCGACGC 
CCTGGAAGGT ACTTTCTGAA GGCCAGCCCA TCTGCAGAAA GTATGCCGCA TTCTTCAGTA 
TCCTCACAAA GACCTAACTC AGATATTCTG ACTCCGGCTT CTCCCTGTGC TTCCACCAAA 
GTCAAGGATT TTAGTTTGTG CAGTAAAACC AAACAAGAGA GAACTCGGTC TGTAACTGTT 
GAGATCCCAG TGGGGAAGAG AACCAGAGAT CTCAATGGGT CCTTATGCCC GAATCAGCCC 
ATTGCACGAA GTATCAGCAC ATTTGGTCCC TTAGATGAAC AGCCATCTCA TGACCAGAGC 
CATGTTATTG CCACTGGCAG CCTCCCAACT TGCTTCCAGG CACCTCCTGA GTCAGGATTG 
GAATGGAAGA TGTCAAATCC AGAGCCAATC ACAGTCCCTT ACCTTAGCCC GCTGGTTGTC 
TGGAAGGAGC TGGAGAGCCT GCTAGAAAAT GAGGGTGACC AATCGATTAC AGTTTCTGGA 
TTTGTGGACC ATCATCCGAT AGTATTCTGG AATTTGGTGT GGTATTTCAG GAGGCTGGAT 
CTCCCAAGTA ACCTCCCGGG ATTAATTCTG TCTTCGGAAC AATGCAATAA AAATTCAAAG 
GTCTCCAGGA GCTGTATGTC TGCAGACAGC AAACATGTCC TTATTCAAGT GCTGTGGGAC 
AATCTGAAAT TGCACCAGGA TCCCCGGCAG CCTCTCTACA TCCTCTGGAA CACTATTAGT 
CAAAACCGCA CTGTATTGTT TGATACACAG GAACATGCGC TTGCTGTGCC ACAGATCAAA 
GAGGAGGAAT TGTTTGATCA GCAGTTTCTC AGAGGGATAG TAAGAAGCAT AAAGAACAAC 
AATGTTTATG GTCCTGTGAG CCAGATATTG GACTGTATTA AACAGGTCCC TGGAAGAACG 
AGGCAGAGGA GTCTGTACAG GGAAATTCTT TTTCTTTCTC TTGTTGCCTT GGGAAGAGAG 
AATATCGATA TTGATGTATT TGATCGGGAA TATAAAACTG CATACGAGCG CCTCACCCCT 
GAGCAGCTGA ACTGCACACA GATTTGTGAT CGGCCTCCCA GCATTGGAGT GATGGAGTGC 
AGGAAGACTT TCGGACAGCC ATTCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755110.1
CDS	48..5897
Translation	MGTFEEEQVSSCPTPKRKMMEDKGPRVADYFVVTGLTDSSKPLEEEIHFNDACHKTAKPKEPITDVTVIIKSLGEEVPQGYVCIDLTPTGLSADLNNGSIRGPQIYLCYRRGRDKPPLTDLGVLYEWKERLKQGCKIIQATPYGRPANISGSASSQRVFITYRRAAESMTQNSLAVTDICIIIPSKGETPPHTFCKVEKNLNSSVWGSAVYLCYKKSVAKTNAVSYKAGLICRYPEEDYESFPLPESVPLFCLPMGATIECWPSDSNYPLPVFSTFVLTGASAEKVYGAAIQFYEPYAQDQLTEKQQVHLGLLSSAEGQMDSSKSIHVNKCICLLSHWPFFDAFKKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDTLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQIEGKRNVTWKILPKKACKNLLNTLCNLYQQLAELQHRPREEAFMELAMNDYDFNYGKRMHQLDMEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFELQGFLKSRDRSHHKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVEKLFSERNNADKGGKADVDRPGETRLIELDESHRSEHTVFITPPEIPHLPNGEEPPKNYSYNGFPVLILDLFDTPDGLMKSHKLSTKVTTPCSPSPMFRRTKQEIRSAHKIAKQYSSIPQMWSRCLLRHCYGLWFICLPAYVKFCHSKVRALRTAYDVLRKMHMKKIDSPDEVCYRVLMQLCGQYGQPVLAVKVLFEMRKVGVQPNAITYGYYNKAVLESVWPSGNRGGYFLWIKVRNVVLAVAQFKQALIKQHASAHQTPASDCSDLDAVSHGSLDSSVDMNPTEQPSFSTDVTCADPADDRSSTGGQSDLGYSSLSKEEVRRGAVHDIQEEKEDSRDSDSSSSSDSESSKSSRDCVPQGTGSQGQQTLASTSSIVRKEAKSDSASSDNSAGLLFTPSFSENCESEVYQCWSLHKRHKSPVESDLKKQMEWEKRNRHLSSGNVEELFISKSDGQNNTENMVDKLGADAKILSTVYKQHMARPKTLDLNKMNPRKSKEVVEKESSDDESPIGGADDKVDSPVIFDLEDLDNDSDELKSAKPCVRKAKLAQRSISMPFSSSMFLAKPAEKTDVKAGLDPLSLLAAETEQAEVEQEEEDDKSLLTPSAKRDLAEEIEMYMNNISSPLASRTPSMDLDRSSSELNKTCKAVALNRRLSVPARSPQEPRLTRSITAHSKRDKRQRSRLWSSPAVSENNPQNEQSPESALALMSPTSFNIDTLLTPTFDVLRSNMFSAGKGVAKKASKWYSKIANYAGPAKDQNSDRASLSSGGAGDTESYSLNDDDFDGSASLHETDVFPGTNTIGVSRCSLESSTSQDSNSKQLYNNGLPSTASPNYKSEILSSCNSSSTSVFQNYAMEVLISSCSRCLTCDGLVHDEEIMAGWTADDSNLNTTCPFCGNSFLPSLNVEIRDLRRPGRYFLKASPSAESMPHSSVSSQRPNSDILTPASPCASTKVKDFSLCSKTKQERTRSVTVEIPVGKRTRDLNGSLCPNQPIARSISTFGPLDEQPSHDQSHVIATGSLPTCFQAPPESGLEWKMSNPEPITVPYLSPLVVWKELESLLENEGDQSITVSGFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEQCNKNSKVSRSCMSADSKHVLIQVLWDNLKLHQDPRQPLYILWNTISQNRTVLFDTQEHALAVPQIKEEELFDQQFLRGIVRSIKNNNVYGPVSQILDCIKQVPGRTRQRSLYREILFLSLVALGRENIDIDVFDREYKTAYERLTPEQLNCTQICDRPPSIGVMECRKTFGQPFL

Target ORF information:

RefSeq Version	XM_031899250.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899250.1

ORF Insert Sequence:

ATGGGAACTT TTGAGGAAGA GCAAGTAAGC AGTTGTCCTA CTCCAAAACG GAAGATGATG 
GAAGACAAAG GGCCCCGTGT GGCTGACTAC TTTGTAGTTA CAGGACTGAC AGATTCATCA 
AAACCACTTG AGGAGGAGAT CCACTTTAAT GATGCCTGTC ATAAAACAGC TAAACCAAAG 
GAACCCATTA CAGATGTGAC TGTGATAATC AAATCCTTAG GGGAGGAAGT GCCACAGGGA 
TATGTATGCA TAGACCTTAC CCCAACTGGA CTATCAGCCG ATCTCAATAA TGGAAGTATT 
CGAGGGCCAC AGATATATCT TTGTTACAGG AGAGGAAGAG ACAAACCACC TCTAACAGAT 
CTTGGGGTAT TGTACGAATG GAAGGAAAGA TTAAAACAGG GCTGCAAGAT TATTCAGGCT 
ACTCCATATG GCCGTCCAGC TAATATAAGC GGTAGTGCAT CCTCACAGAG AGTATTTATC 
ACATACCGCA GAGCTGCTGA GAGCATGACC CAGAATTCCT TGGCTGTAAC TGATATCTGC 
ATCATTATAC CAAGCAAAGG AGAGACTCCA CCACACACAT TTTGCAAAGT GGAAAAGAAT 
CTGAACAGCA GCGTGTGGGG CTCTGCTGTG TACCTGTGCT ACAAGAAATC AGTTGCCAAA 
ACAAATGCAG TATCCTATAA AGCTGGGTTG ATATGCAGGT ATCCAGAGGA GGATTATGAA 
TCATTTCCTC TGCCGGAATC AGTCCCACTA TTTTGTCTAC CCATGGGGGC AACAATTGAA 
TGTTGGCCAT CTGACAGTAA TTATCCACTC CCTGTTTTTT CCACTTTTGT TTTAACTGGA 
GCATCAGCTG AAAAGGTGTA TGGAGCTGCT ATTCAGTTTT ATGAGCCATA TGCTCAAGAT 
CAGCTGACTG AAAAGCAACA AGTACATTTA GGCCTCTTGA GCAGTGCAGA GGGTCAGATG 
GACTCTTCTA AAAGTATCCA TGTCAACAAG TGCATCTGCC TGCTGTCCCA CTGGCCTTTT 
TTTGATGCTT TCAAGAAATT TCTCACTTTC CTTTACCGCT ATTCAATATC GGGTCCTCAT 
GTGCTGCCAA TAGAAAAACA TATCTCCCAC TTCATGCACA AAGTCCCATT CCCATCTCCA 
CAGAGACCAC GCATTCTAGT TCAGCTTTCC CCGCATGATA CTCTGATCCT AAGCCAGCCT 
GTGTCCTCAC CACTTCCACT GAGTGGTGGA AAGTTTTCAA CGTTGCTGCA GAATTTGGGA 
CCTGAAAATG CTGTGACTCT GCTTGTATTT GCTGTCACGG AGCATAAGAT TCTGATCCAT 
TCGTTGCGCC CCTCGGTTCT CACAAGCGTC ACAGAAGCTT TAGTGTCTAT GATCTTCCCT 
TTCCATTGGC CGTGTCCTTA CATTCCACTT TGCCCTTTGG CCCTGGCAGA CGTACTAAGC 
GCCCCATGCC CCTTCATCGT GGGAATTGAT TCTCGCTACT TTGATCTGTA CGACCCCCCT 
CCGGATGTAA GCTGTGTTGA CCTAGATACC AACACCATCT CTCAAATTGA AGGCAAAAGA 
AATGTAACAT GGAAAATCCT ACCGAAGAAA GCTTGTAAGA ACCTGCTCAA CACTCTTTGT 
AACTTGTACC AGCAACTGGC AGAGTTGCAA CACAGACCAC GGGAAGAAGC TTTTATGGAA 
CTTGCAATGA ATGACTATGA CTTTAACTAT GGAAAAAGAA TGCATCAGCT GGACATGGAG 
ATTCAGGAAG CATTCCTGTG CTTTATGGCC TCTATTTTAA AAGGATACAG ATCGTATCTT 
CGGCCAATTA CCCAAGCACC ATCAGAGACT GCAACAGATG CCAGCTCTCT CTTCGAACTG 
CAAGGTTTCT TAAAAAGTCG TGATCGCTCA CACCACAAGT TTTACAACAT GATGACCAAG 
ACTCAGATGT TCATCCGCTT TATTGAGGAG TGTTCCTTCG TCAGCGATAA GGATGCTAGC 
CTGGCTTTCT TTGATGACTG TGTTGAGAAG CTTTTCTCAG AGAGGAATAA TGCAGATAAA 
GGTGGCAAGG CGGATGTGGA TCGACCAGGA GAGACACGTC TTATTGAACT GGATGAGTCT 
CACAGAAGTG AGCATACAGT GTTCATCACA CCACCAGAGA TTCCCCACTT ACCAAATGGC 
GAGGAGCCCC CTAAGAACTA TAGCTACAAT GGGTTCCCTG TCCTTATCTT GGATCTCTTT 
GACACGCCAG ATGGACTCAT GAAAAGTCAC AAACTTAGTA CAAAAGTCAC CACACCCTGC 
AGTCCTTCTC CAATGTTTAG AAGAACAAAA CAGGAAATCC GGTCAGCACA TAAAATAGCA 
AAGCAGTACT CCTCCATCCC CCAGATGTGG TCCAGGTGTC TACTGAGACA TTGCTATGGA 
CTTTGGTTTA TCTGTCTGCC CGCATATGTG AAATTCTGCC ATTCCAAAGT GAGAGCACTG 
CGCACAGCCT ACGATGTGTT GCGAAAAATG CACATGAAAA AGATTGACTC TCCTGATGAG 
GTTTGCTATA GGGTTCTAAT GCAGTTGTGT GGCCAATATG GGCAGCCTGT CCTTGCTGTT 
AAAGTTCTCT TTGAAATGAG AAAGGTCGGC GTGCAGCCAA ATGCTATAAC ATATGGCTAT 
TATAATAAGG CTGTCCTGGA AAGCGTATGG CCCTCTGGGA ATAGAGGGGG TTACTTTCTG 
TGGATAAAGG TGAGGAATGT GGTGCTAGCA GTAGCCCAGT TCAAACAGGC TTTGATAAAG 
CAACATGCTT CAGCTCATCA AACTCCTGCT TCAGATTGCA GTGATTTGGA CGCAGTCAGC 
CATGGCAGTT TGGATAGTTC TGTTGACATG AACCCTACAG AACAGCCTTC ATTCAGTACA 
GATGTAACAT GTGCTGATCC AGCTGACGAC CGATCTAGTA CAGGAGGCCA GTCAGACCTT 
GGCTACAGTT CATTATCAAA AGAAGAAGTA AGAAGAGGAG CCGTGCATGA CATACAGGAG 
GAGAAGGAAG ACTCCAGAGA TAGTGACTCC AGTTCGTCAT CTGATTCAGA AAGTTCCAAG 
AGCAGCAGAG ACTGTGTGCC ACAAGGAACT GGCAGTCAGG GTCAACAAAC ATTGGCAAGC 
ACATCTAGTA TAGTCCGGAA AGAAGCAAAA TCAGACAGTG CAAGCAGTGA CAACTCAGCA 
GGTCTACTGT TCACACCATC CTTTAGTGAG AATTGTGAAA GTGAAGTGTA TCAGTGCTGG 
AGTCTACATA AAAGACACAA GAGTCCTGTG GAGTCAGATC TGAAAAAACA GATGGAATGG 
GAAAAGAGGA ACAGGCATCT AAGCAGTGGC AATGTTGAGG AATTATTTAT CAGTAAGTCT 
GATGGACAGA ATAACACTGA AAACATGGTG GACAAACTTG GAGCAGATGC AAAAATACTT 
AGCACTGTAT ATAAGCAACA CATGGCACGG CCCAAGACCC TGGACTTAAA TAAAATGAAT 
CCCAGAAAGT CAAAGGAGGT TGTTGAAAAA GAATCTAGTG ATGATGAGTC TCCCATAGGA 
GGAGCAGATG ACAAAGTGGA CTCCCCTGTT ATTTTTGACC TTGAAGATCT AGATAATGAT 
TCTGATGAAC TTAAATCAGC AAAACCATGT GTCCGGAAGG CCAAACTTGC TCAAAGAAGT 
ATCAGCATGC CCTTTTCATC TTCCATGTTT CTGGCTAAGC CAGCTGAAAA AACAGACGTG 
AAGGCTGGAT TAGACCCTTT GTCATTGCTG GCAGCTGAAA CAGAACAGGC AGAAGTAGAA 
CAGGAGGAGG AAGATGACAA AAGCTTACTA ACACCATCTG CTAAAAGGGA CCTTGCAGAG 
GAAATAGAAA TGTACATGAA CAATATAAGC AGCCCCCTTG CAAGTCGAAC CCCTAGCATG 
GACCTTGACA GATCCAGTTC TGAACTAAAT AAAACATGTA AAGCTGTAGC ACTCAACAGG 
CGCTTGAGTG TTCCTGCTAG ATCCCCACAA GAACCAAGAC TAACCAGATC AATTACTGCC 
CACAGTAAAC GCGACAAAAG ACAAAGAAGC AGGCTCTGGT CTTCTCCTGC TGTTTCCGAG 
AACAATCCTC AGAATGAGCA AAGCCCAGAA TCTGCCCTTG CTTTAATGTC CCCAACATCA 
TTTAACATAG ATACACTTCT CACGCCAACC TTTGATGTTC TTAGGAGCAA CATGTTCTCA 
GCTGGCAAAG GAGTAGCCAA GAAGGCCAGC AAATGGTATT CAAAGATCGC AAATTATGCG 
GGGCCTGCAA AGGATCAGAA TTCTGACCGA GCCAGTCTGT CTTCAGGAGG AGCAGGGGAT 
ACTGAATCCT ACTCTTTAAA TGATGATGAT TTTGATGGCA GTGCTTCTCT GCATGAGACT 
GATGTCTTTC CTGGAACCAA TACCATTGGT GTAAGCAGAT GCAGCTTGGA GAGTAGCACA 
TCTCAAGACA GTAACTCTAA ACAACTTTAC AATAATGGTC TGCCATCAAC AGCATCTCCA 
AACTACAAGA GTGAGATTCT TTCATCCTGC AATTCCAGTA GCACTAGTGT CTTCCAGAAC 
TATGCTATGG AGGTTCTGAT ATCTAGCTGT TCACGTTGCT TGACCTGTGA CGGTCTTGTT 
CATGATGAGG AGATTATGGC AGGCTGGACG GCAGATGACT CAAACCTTAA CACCACATGT 
CCTTTTTGCG GGAACTCATT TCTTCCATCT TTAAACGTTG AAATTAGGGA CCTGCGACGC 
CCTGGAAGGT ACTTTCTGAA GGCCAGCCCA TCTGCAGAAA GTATGCCGCA TTCTTCAGTA 
TCCTCACAAA GACCTAACTC AGATATTCTG ACTCCGGCTT CTCCCTGTGC TTCCACCAAA 
GTCAAGGATT TTAGTTTGTG CAGTAAAACC AAACAAGAGA GAACTCGGTC TGTAACTGTT 
GAGATCCCAG TGGGGAAGAG AACCAGAGAT CTCAATGGGT CCTTATGCCC GAATCAGCCC 
ATTGCACGAA GTATCAGCAC ATTTGGTCCC TTAGATGAAC AGCCATCTCA TGACCAGAGC 
CATGTTATTG CCACTGGCAG CCTCCCAACT TGCTTCCAGG CACCTCCTGA GTCAGGATTG 
GAATGGAAGA TGTCAAATCC AGAGCCAATC ACAGTCCCTT ACCTTAGCCC GCTGGTTGTC 
TGGAAGGAGC TGGAGAGCCT GCTAGAAAAT GAGGGTGACC AATCGATTAC AGTTTCTGGA 
TTTGTGGACC ATCATCCGAT AGTATTCTGG AATTTGGTGT GGTATTTCAG GAGGCTGGAT 
CTCCCAAGTA ACCTCCCGGG ATTAATTCTG TCTTCGGAAC AATGCAATAA AAATTCAAAG 
GTCTCCAGGA GCTGTATGTC TGCAGACAGC AAACATGTCC TTATTCAAGT GCTGTGGGAC 
AATCTGAAAT TGCACCAGGA TCCCCGGCAG CCTCTCTACA TCCTCTGGAA CACTATTAGT 
CAAAACCGCA CTGTATTGTT TGATACACAG GAACATGCGC TTGCTGTGCC ACAGATCAAA 
GAGGAGGAAT TGTTTGATCA GCAGTTTCTC AGAGGGATAG TAAGAAGCAT AAAGAACAAC 
AATGTTTATG GTCCTGTGAG CCAGATATTG GACTGTATTA AACAGGTCCC TGGAAGAACG 
AGGCAGAGGA GTCTGTACAG GGAAATTCTT TTTCTTTCTC TTGTTGCCTT GGGAAGAGAG 
AATATCGATA TTGATGTATT TGATCGGGAA TATAAAACTG CATACGAGCG CCTCACCCCT 
GAGCAGCTGA ACTGCACACA GATTTGTGAT CGGCCTCCCA GCATTGGAGT GATGGAGTGC 
AGGAAGACTT TCGGACAGCC ATTCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4a ( XM_031899251.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755111.1 Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X2, mRNA.

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CloneID	OXa55246
Clone ID Related Accession (Same CDS sequence)	XM_031899251.1
Accession Version	XM_031899251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	C-myc promoter-binding protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACTT TTGAGGAAGA GCAAGTAAGC AGTTGTCCTA CTCCAAAACG GAAGATGATG 
GAAGACAAAG GGCCCCGTGT GGCTGACTAC TTTGTAGTTA CAGGACTGAC AGATTCATCA 
AAACCACTTG AGGAGGAGAT CCACTTTAAT GATGCCTGTC ATAAAACAGC TAAACCAAAG 
GAACCCATTA CAGATGTGAC TGTGATAATC AAATCCTTAG GGGAGGAAGT GCCACAGGGA 
TATGTATGCA TAGACCTTAC CCCAACTGGA CTATCAGCCG ATCTCAATAA TGGAAGTATT 
CGAGGGCCAC AGATATATCT TTGTTACAGG AGAGGAAGAG ACAAACCACC TCTAACAGAT 
CTTGGGGTAT TGTACGAATG GAAGGAAAGA TTAAAACAGG GCTGCAAGAT TATTCAGGCT 
ACTCCATATG GCCGTCCAGC TAATATAAGC GGTAGTGCAT CCTCACAGAG AGTATTTATC 
ACATACCGCA GAGCTGCTGA GAGCATGACC CAGAATTCCT TGGCTGTAAC TGATATCTGC 
ATCATTATAC CAAGCAAAGG AGAGACTCCA CCACACACAT TTTGCAAAGT GGAAAAGAAT 
CTGAACAGCA GCGTGTGGGG CTCTGCTGTG TACCTGTGCT ACAAGAAATC AGTTGCCAAA 
ACAAATGCAG TATCCTATAA AGCTGGGTTG ATATGCAGGT ATCCAGAGGA GGATTATGAA 
TCATTTCCTC TGCCGGAATC AGTCCCACTA TTTTGTCTAC CCATGGGGGC AACAATTGAA 
TGTTGGCCAT CTGACAGTAA TTATCCACTC CCTGTTTTTT CCACTTTTGT TTTAACTGGA 
GCATCAGCTG AAAAGGTGTA TGGAGCTGCT ATTCAGTTTT ATGAGCCATA TGCTCAAGAT 
CAGCTGACTG AAAAGCAACA AGTACATTTA GGCCTCTTGA GCAGTGCAGA GGGTCAGATG 
GACTCTTCTA AAAGTATCCA TGTCAACAAG TGCATCTGCC TGCTGTCCCA CTGGCCTTTT 
TTTGATGCTT TCAAGAAATT TCTCACTTTC CTTTACCGCT ATTCAATATC GGGTCCTCAT 
GTGCTGCCAA TAGAAAAACA TATCTCCCAC TTCATGCACA AAGTCCCATT CCCATCTCCA 
CAGAGACCAC GCATTCTAGT TCAGCTTTCC CCGCATGATA CTCTGATCCT AAGCCAGCCT 
GTGTCCTCAC CACTTCCACT GAGTGGTGGA AAGTTTTCAA CGTTGCTGCA GAATTTGGGA 
CCTGAAAATG CTGTGACTCT GCTTGTATTT GCTGTCACGG AGCATAAGAT TCTGATCCAT 
TCGTTGCGCC CCTCGGTTCT CACAAGCGTC ACAGAAGCTT TAGTGTCTAT GATCTTCCCT 
TTCCATTGGC CGTGTCCTTA CATTCCACTT TGCCCTTTGG CCCTGGCAGA CGTACTAAGC 
GCCCCATGCC CCTTCATCGT GGGAATTGAT TCTCGCTACT TTGATCTGTA CGACCCCCCT 
CCGGATGTAA GCTGTGTTGA CCTAGATACC AACACCATCT CTCAAATTGA AGGCAAAAGA 
AATGTAACAT GGAAAATCCT ACCGAAGAAA GCTTGTAAGA ACCTGCTCAA CACTCTTTGT 
AACTTGTACC AGCAACTGGC AGAGTTGCAA CACAGACCAC GGGAAGAAGC TTTTATGGAA 
CTTGCAATGA ATGACTATGA CTTTAACTAT GGAAAAAGAA TGCATCAGCT GGACATGGAG 
ATTCAGGAAG CATTCCTGTG CTTTATGGCC TCTATTTTAA AAGGATACAG ATCGTATCTT 
CGGCCAATTA CCCAAGCACC ATCAGAGACT GCAACAGATG CCAGCTCTCT CTTCGAACTG 
CAAGGTTTCT TAAAAAGTCG TGATCGCTCA CACCACAAGT TTTACAACAT GATGACCAAG 
ACTCAGATGT TCATCCGCTT TATTGAGGAG TGTTCCTTCG TCAGCGATAA GGATGCTAGC 
CTGGCTTTCT TTGATGACTG TGTTGAGAAG CTTTTCTCAG AGAGGAATAA TGCAGATAAA 
GGTGGCAAGG CGGATGTGGA TCGACCAGGA GAGACACGTC TTATTGAACT GGATGAGTCT 
CACAGAAGTG AGCATACAGT GTTCATCACA CCACCAGAGA TTCCCCACTT ACCAAATGGC 
GAGGAGCCCC CTAAGAACTA TAGCTACAAT GGGTTCCCTG TCCTTATCTT GGATCTCTTT 
GACACGCCAG ATGGACTCAT GAAAAGTCAC AAACTTAGTA CAAAAGTCAC CACACCCTGC 
AGTCCTTCTC CAATGTTTAG AAGAACAAAA CAGGAAATCC GGTCAGCACA TAAAATAGCA 
AAGCAGTACT CCTCCATCCC CCAGATGTGG TCCAGGTGTC TACTGAGACA TTGCTATGGA 
CTTTGGTTTA TCTGTCTGCC CGCATATGTG AAATTCTGCC ATTCCAAAGT GAGAGCACTG 
CGCACAGCCT ACGATGTGTT GCGAAAAATG CACATGAAAA AGATTGACTC TCCTGATGAG 
GTTTGCTATA GGGTTCTAAT GCAGTTGTGT GGCCAATATG GGCAGCCTGT CCTTGCTGTT 
AAAGTTCTCT TTGAAATGAG AAAGGTCGGC GTGCAGCCAA ATGCTATAAC ATATGGCTAT 
TATAATAAGG CTGTCCTGGA AAGCGTATGG CCCTCTGGGA ATAGAGGGGG TTACTTTCTG 
TGGATAAAGG TGAGGAATGT GGTGCTAGCA GTAGCCCAGT TCAAACAGGC TTTGATAAAG 
CAACATGCTT CAGCTCATCA AACTCCTGCT TCAGATTGCA GTGATTTGGA CGCAGTCAGC 
CATGGCAGTT TGGATAGTTC TGTTGACATG AACCCTACAG AACAGCCTTC ATTCAGTACA 
GATGTAACAT GTGCTGATCC AGCTGACGAC CGATCTAGTA CAGGAGGCCA GTCAGACCTT 
GGCTACAGTT CATTATCAAA AGAAGAAGTA AGAAGAGGAG CCGTGCATGA CATACAGGAG 
GAGAAGGAAG ACTCCAGAGA TAGTGACTCC AGTTCGTCAT CTGATTCAGA AAGTTCCAAG 
AGCAGCAGAG ACTGTGTGCC ACAAGGAACT GGCAGTCAGG GTCAACAAAC ATTGGCAAGC 
ACATCTAGTA TAGTCCGGAA AGAAGCAAAA TCAGACAGTG CAAGCAGTGA CAACTCAGCA 
GGTCTACTGT TCACACCATC CTTTAGTGAG AATTGTGAAA GTGAAGTGTA TCAGTGCTGG 
AGTCTACATA AAAGACACAA GAGTCCTGTG GAGTCAGATC TGAAAAAACA GATGGAATGG 
GAAAAGAGGA ACAGGCATCT AAGCAGTGGC AATGTTGAGG AATTATTTAT CAGTAAGTCT 
GATGGACAGA ATAACACTGA AAACATGGTG GACAAACTTG GAGCAGATGC AAAAATACTT 
AGCACTGTAT ATAAGCAACA CATGGCACGG CCCAAGACCC TGGACTTAAA TAAAATGAAT 
CCCAGAAAGT CAAAGGAGGT TGTTGAAAAA GAATCTAGTG ATGATGAGTC TCCCATAGGA 
GGAGCAGATG ACAAAGTGGA CTCCCCTGTT ATTTTTGACC TTGAAGATCT AGATAATGAT 
TCTGATGAAC TTAAATCAGC AAAACCATGT GTCCGGAAGG CCAAACTTGC TCAAAGAAGT 
ATCAGCATGC CCTTTTCATC TTCCATGTTT CTGGCTAAGC CAGCTGAAAA AACAGACGTG 
AAGGCTGGAT TAGACCCTTT GTCATTGCTG GCAGCTGAAA CAGAACAGGC AGAAGTAGAA 
CAGGAGGAGG AAGATGACAA AAGCTTACTA ACACCATCTG CTAAAAGGGA CCTTGCAGAG 
GAAATAGAAA TGTACATGAA CAATATAAGC AGCCCCCTTG CAAGTCGAAC CCCTAGCATG 
GACCTTGACA GATCCAGTTC TGAACTAAAT AAAACATGTA AAGCTGTAGC ACTCAACAGG 
CGCTTGAGTG TTCCTGCTAG ATCCCCACAA GAACCAAGAC TAACCAGATC AATTACTGCC 
CACAGTAAAC GCGACAAAAG ACAAAGAAGC AGGCTCTGGT CTTCTCCTGC TGTTTCCGAG 
AACAATCCTC AGAATGAGCA AAGCCCAGAA TCTGCCCTTG CTTTAATGTC CCCAACATCA 
TTTAACATAG ATACACTTCT CACGCCAACC TTTGATGTTC TTAGGAGCAA CATGTTCTCA 
GCTGGCAAAG GAGTAGCCAA GAAGGCCAGC AAATGGTATT CAAAGATCGC AAATTATGCG 
GGGCCTGCAA AGGATCAGAA TTCTGACCGA GCCAGTCTGT CTTCAGGAGG AGCAGGGGAT 
ACTGAATCCT ACTCTTTAAA TGATGATGAT TTTGATGGCA GTGCTTCTCT GCATGAGACT 
GATGTCTTTC CTGGAACCAA TACCATTGGT GTAAGCAGAT GCAGCTTGGA GAGTAGCACA 
TCTCAAGACA GTAACTCTAA ACAACTTTAC AATAATGGTC TGCCATCAAC AGCATCTCCA 
AACTACAAGA GTGAGATTCT TTCATCCTGC AATTCCAGTA GCACTAGTGT CTTCCAGAAC 
TATGCTATGG AGGTTCTGAT ATCTAGCTGT TCACGTTGCT TGACCTGTGA CGGTCTTGTT 
CATGATGAGG AGATTATGGC AGGCTGGACG GCAGATGACT CAAACCTTAA CACCACATGT 
CCTTTTTGCG GGAACTCATT TCTTCCATCT TTAAACGTTG AAATTAGGGA CCTGCGACGC 
CCTGGAAGGT ACTTTCTGAA GGCCAGCCCA TCTGCAGAAA GTATGCCGCA TTCTTCAGTA 
TCCTCACAAA GACCTAACTC AGATATTCTG ACTCCGGCTT CTCCCTGTGC TTCCACCAAA 
GTCAAGGATT TTAGTTTGTG CAGTAAAACC AAACAAGAGA GAACTCGGTC TGTAACTGTT 
GAGATCCCAG TGGGGAAGAG AACCAGAGAT CTCAATGGGT CCTTATGCCC GAATCAGCCC 
ATTGCACGAA GTATCAGCAC ATTTGGTCCC TTAGATGAAC AGCCATCTCA TGACCAGAGC 
CATGTTATTG CCACTGGCAG CCTCCCAACT TGCTTCCAGG CACCTCCTGA GTCAGGATTG 
GAATGGAAGA TGTCAAATCC AGAGCCAATC ACAGTCCCTT ACCTTAGCCC GCTGGTTGTC 
TGGAAGGAGC TGGAGAGCCT GCTAGAAAAT GAGGGTGACC AATCGATTAC AGTTTCTGGA 
TTTGTGGACC ATCATCCGAT AGTATTCTGG AATTTGGTGT GGTATTTCAG GAGGCTGGAT 
CTCCCAAGTA ACCTCCCGGG ATTAATTCTG TCTTCGGAAC AATGCAATAA AAATTCAAAG 
GTCTCCAGGA GCTGTATGTC TGCAGACAGC AAACATGTCC TTATTCAAGT GCTGTGGGAC 
AATCTGAAAT TGCACCAGGA TCCCCGGCAG CCTCTCTACA TCCTCTGGAA CACTATTACA 
CAGGAACATG CGCTTGCTGT GCCACAGATC AAAGAGGAGG AATTGTTTGA TCAGCAGTTT 
CTCAGAGGGA TAGTAAGAAG CATAAAGAAC AACAATGTTT ATGGTCCTGT GAGCCAGATA 
TTGGACTGTA TTAAACAGGT CCCTGGAAGA ACGAGGCAGA GGAGTCTGTA CAGGGAAATT 
CTTTTTCTTT CTCTTGTTGC CTTGGGAAGA GAGAATATCG ATATTGATGT ATTTGATCGG 
GAATATAAAA CTGCATACGA GCGCCTCACC CCTGAGCAGC TGAACTGCAC ACAGATTTGT 
GATCGGCCTC CCAGCATTGG AGTGATGGAG TGCAGGAAGA CTTTCGGACA GCCATTCCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755111.1
CDS	48..5870
Translation	MGTFEEEQVSSCPTPKRKMMEDKGPRVADYFVVTGLTDSSKPLEEEIHFNDACHKTAKPKEPITDVTVIIKSLGEEVPQGYVCIDLTPTGLSADLNNGSIRGPQIYLCYRRGRDKPPLTDLGVLYEWKERLKQGCKIIQATPYGRPANISGSASSQRVFITYRRAAESMTQNSLAVTDICIIIPSKGETPPHTFCKVEKNLNSSVWGSAVYLCYKKSVAKTNAVSYKAGLICRYPEEDYESFPLPESVPLFCLPMGATIECWPSDSNYPLPVFSTFVLTGASAEKVYGAAIQFYEPYAQDQLTEKQQVHLGLLSSAEGQMDSSKSIHVNKCICLLSHWPFFDAFKKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDTLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQIEGKRNVTWKILPKKACKNLLNTLCNLYQQLAELQHRPREEAFMELAMNDYDFNYGKRMHQLDMEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFELQGFLKSRDRSHHKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVEKLFSERNNADKGGKADVDRPGETRLIELDESHRSEHTVFITPPEIPHLPNGEEPPKNYSYNGFPVLILDLFDTPDGLMKSHKLSTKVTTPCSPSPMFRRTKQEIRSAHKIAKQYSSIPQMWSRCLLRHCYGLWFICLPAYVKFCHSKVRALRTAYDVLRKMHMKKIDSPDEVCYRVLMQLCGQYGQPVLAVKVLFEMRKVGVQPNAITYGYYNKAVLESVWPSGNRGGYFLWIKVRNVVLAVAQFKQALIKQHASAHQTPASDCSDLDAVSHGSLDSSVDMNPTEQPSFSTDVTCADPADDRSSTGGQSDLGYSSLSKEEVRRGAVHDIQEEKEDSRDSDSSSSSDSESSKSSRDCVPQGTGSQGQQTLASTSSIVRKEAKSDSASSDNSAGLLFTPSFSENCESEVYQCWSLHKRHKSPVESDLKKQMEWEKRNRHLSSGNVEELFISKSDGQNNTENMVDKLGADAKILSTVYKQHMARPKTLDLNKMNPRKSKEVVEKESSDDESPIGGADDKVDSPVIFDLEDLDNDSDELKSAKPCVRKAKLAQRSISMPFSSSMFLAKPAEKTDVKAGLDPLSLLAAETEQAEVEQEEEDDKSLLTPSAKRDLAEEIEMYMNNISSPLASRTPSMDLDRSSSELNKTCKAVALNRRLSVPARSPQEPRLTRSITAHSKRDKRQRSRLWSSPAVSENNPQNEQSPESALALMSPTSFNIDTLLTPTFDVLRSNMFSAGKGVAKKASKWYSKIANYAGPAKDQNSDRASLSSGGAGDTESYSLNDDDFDGSASLHETDVFPGTNTIGVSRCSLESSTSQDSNSKQLYNNGLPSTASPNYKSEILSSCNSSSTSVFQNYAMEVLISSCSRCLTCDGLVHDEEIMAGWTADDSNLNTTCPFCGNSFLPSLNVEIRDLRRPGRYFLKASPSAESMPHSSVSSQRPNSDILTPASPCASTKVKDFSLCSKTKQERTRSVTVEIPVGKRTRDLNGSLCPNQPIARSISTFGPLDEQPSHDQSHVIATGSLPTCFQAPPESGLEWKMSNPEPITVPYLSPLVVWKELESLLENEGDQSITVSGFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEQCNKNSKVSRSCMSADSKHVLIQVLWDNLKLHQDPRQPLYILWNTITQEHALAVPQIKEEELFDQQFLRGIVRSIKNNNVYGPVSQILDCIKQVPGRTRQRSLYREILFLSLVALGRENIDIDVFDREYKTAYERLTPEQLNCTQICDRPPSIGVMECRKTFGQPFL

Target ORF information:

RefSeq Version	XM_031899251.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899251.1

ORF Insert Sequence:

ATGGGAACTT TTGAGGAAGA GCAAGTAAGC AGTTGTCCTA CTCCAAAACG GAAGATGATG 
GAAGACAAAG GGCCCCGTGT GGCTGACTAC TTTGTAGTTA CAGGACTGAC AGATTCATCA 
AAACCACTTG AGGAGGAGAT CCACTTTAAT GATGCCTGTC ATAAAACAGC TAAACCAAAG 
GAACCCATTA CAGATGTGAC TGTGATAATC AAATCCTTAG GGGAGGAAGT GCCACAGGGA 
TATGTATGCA TAGACCTTAC CCCAACTGGA CTATCAGCCG ATCTCAATAA TGGAAGTATT 
CGAGGGCCAC AGATATATCT TTGTTACAGG AGAGGAAGAG ACAAACCACC TCTAACAGAT 
CTTGGGGTAT TGTACGAATG GAAGGAAAGA TTAAAACAGG GCTGCAAGAT TATTCAGGCT 
ACTCCATATG GCCGTCCAGC TAATATAAGC GGTAGTGCAT CCTCACAGAG AGTATTTATC 
ACATACCGCA GAGCTGCTGA GAGCATGACC CAGAATTCCT TGGCTGTAAC TGATATCTGC 
ATCATTATAC CAAGCAAAGG AGAGACTCCA CCACACACAT TTTGCAAAGT GGAAAAGAAT 
CTGAACAGCA GCGTGTGGGG CTCTGCTGTG TACCTGTGCT ACAAGAAATC AGTTGCCAAA 
ACAAATGCAG TATCCTATAA AGCTGGGTTG ATATGCAGGT ATCCAGAGGA GGATTATGAA 
TCATTTCCTC TGCCGGAATC AGTCCCACTA TTTTGTCTAC CCATGGGGGC AACAATTGAA 
TGTTGGCCAT CTGACAGTAA TTATCCACTC CCTGTTTTTT CCACTTTTGT TTTAACTGGA 
GCATCAGCTG AAAAGGTGTA TGGAGCTGCT ATTCAGTTTT ATGAGCCATA TGCTCAAGAT 
CAGCTGACTG AAAAGCAACA AGTACATTTA GGCCTCTTGA GCAGTGCAGA GGGTCAGATG 
GACTCTTCTA AAAGTATCCA TGTCAACAAG TGCATCTGCC TGCTGTCCCA CTGGCCTTTT 
TTTGATGCTT TCAAGAAATT TCTCACTTTC CTTTACCGCT ATTCAATATC GGGTCCTCAT 
GTGCTGCCAA TAGAAAAACA TATCTCCCAC TTCATGCACA AAGTCCCATT CCCATCTCCA 
CAGAGACCAC GCATTCTAGT TCAGCTTTCC CCGCATGATA CTCTGATCCT AAGCCAGCCT 
GTGTCCTCAC CACTTCCACT GAGTGGTGGA AAGTTTTCAA CGTTGCTGCA GAATTTGGGA 
CCTGAAAATG CTGTGACTCT GCTTGTATTT GCTGTCACGG AGCATAAGAT TCTGATCCAT 
TCGTTGCGCC CCTCGGTTCT CACAAGCGTC ACAGAAGCTT TAGTGTCTAT GATCTTCCCT 
TTCCATTGGC CGTGTCCTTA CATTCCACTT TGCCCTTTGG CCCTGGCAGA CGTACTAAGC 
GCCCCATGCC CCTTCATCGT GGGAATTGAT TCTCGCTACT TTGATCTGTA CGACCCCCCT 
CCGGATGTAA GCTGTGTTGA CCTAGATACC AACACCATCT CTCAAATTGA AGGCAAAAGA 
AATGTAACAT GGAAAATCCT ACCGAAGAAA GCTTGTAAGA ACCTGCTCAA CACTCTTTGT 
AACTTGTACC AGCAACTGGC AGAGTTGCAA CACAGACCAC GGGAAGAAGC TTTTATGGAA 
CTTGCAATGA ATGACTATGA CTTTAACTAT GGAAAAAGAA TGCATCAGCT GGACATGGAG 
ATTCAGGAAG CATTCCTGTG CTTTATGGCC TCTATTTTAA AAGGATACAG ATCGTATCTT 
CGGCCAATTA CCCAAGCACC ATCAGAGACT GCAACAGATG CCAGCTCTCT CTTCGAACTG 
CAAGGTTTCT TAAAAAGTCG TGATCGCTCA CACCACAAGT TTTACAACAT GATGACCAAG 
ACTCAGATGT TCATCCGCTT TATTGAGGAG TGTTCCTTCG TCAGCGATAA GGATGCTAGC 
CTGGCTTTCT TTGATGACTG TGTTGAGAAG CTTTTCTCAG AGAGGAATAA TGCAGATAAA 
GGTGGCAAGG CGGATGTGGA TCGACCAGGA GAGACACGTC TTATTGAACT GGATGAGTCT 
CACAGAAGTG AGCATACAGT GTTCATCACA CCACCAGAGA TTCCCCACTT ACCAAATGGC 
GAGGAGCCCC CTAAGAACTA TAGCTACAAT GGGTTCCCTG TCCTTATCTT GGATCTCTTT 
GACACGCCAG ATGGACTCAT GAAAAGTCAC AAACTTAGTA CAAAAGTCAC CACACCCTGC 
AGTCCTTCTC CAATGTTTAG AAGAACAAAA CAGGAAATCC GGTCAGCACA TAAAATAGCA 
AAGCAGTACT CCTCCATCCC CCAGATGTGG TCCAGGTGTC TACTGAGACA TTGCTATGGA 
CTTTGGTTTA TCTGTCTGCC CGCATATGTG AAATTCTGCC ATTCCAAAGT GAGAGCACTG 
CGCACAGCCT ACGATGTGTT GCGAAAAATG CACATGAAAA AGATTGACTC TCCTGATGAG 
GTTTGCTATA GGGTTCTAAT GCAGTTGTGT GGCCAATATG GGCAGCCTGT CCTTGCTGTT 
AAAGTTCTCT TTGAAATGAG AAAGGTCGGC GTGCAGCCAA ATGCTATAAC ATATGGCTAT 
TATAATAAGG CTGTCCTGGA AAGCGTATGG CCCTCTGGGA ATAGAGGGGG TTACTTTCTG 
TGGATAAAGG TGAGGAATGT GGTGCTAGCA GTAGCCCAGT TCAAACAGGC TTTGATAAAG 
CAACATGCTT CAGCTCATCA AACTCCTGCT TCAGATTGCA GTGATTTGGA CGCAGTCAGC 
CATGGCAGTT TGGATAGTTC TGTTGACATG AACCCTACAG AACAGCCTTC ATTCAGTACA 
GATGTAACAT GTGCTGATCC AGCTGACGAC CGATCTAGTA CAGGAGGCCA GTCAGACCTT 
GGCTACAGTT CATTATCAAA AGAAGAAGTA AGAAGAGGAG CCGTGCATGA CATACAGGAG 
GAGAAGGAAG ACTCCAGAGA TAGTGACTCC AGTTCGTCAT CTGATTCAGA AAGTTCCAAG 
AGCAGCAGAG ACTGTGTGCC ACAAGGAACT GGCAGTCAGG GTCAACAAAC ATTGGCAAGC 
ACATCTAGTA TAGTCCGGAA AGAAGCAAAA TCAGACAGTG CAAGCAGTGA CAACTCAGCA 
GGTCTACTGT TCACACCATC CTTTAGTGAG AATTGTGAAA GTGAAGTGTA TCAGTGCTGG 
AGTCTACATA AAAGACACAA GAGTCCTGTG GAGTCAGATC TGAAAAAACA GATGGAATGG 
GAAAAGAGGA ACAGGCATCT AAGCAGTGGC AATGTTGAGG AATTATTTAT CAGTAAGTCT 
GATGGACAGA ATAACACTGA AAACATGGTG GACAAACTTG GAGCAGATGC AAAAATACTT 
AGCACTGTAT ATAAGCAACA CATGGCACGG CCCAAGACCC TGGACTTAAA TAAAATGAAT 
CCCAGAAAGT CAAAGGAGGT TGTTGAAAAA GAATCTAGTG ATGATGAGTC TCCCATAGGA 
GGAGCAGATG ACAAAGTGGA CTCCCCTGTT ATTTTTGACC TTGAAGATCT AGATAATGAT 
TCTGATGAAC TTAAATCAGC AAAACCATGT GTCCGGAAGG CCAAACTTGC TCAAAGAAGT 
ATCAGCATGC CCTTTTCATC TTCCATGTTT CTGGCTAAGC CAGCTGAAAA AACAGACGTG 
AAGGCTGGAT TAGACCCTTT GTCATTGCTG GCAGCTGAAA CAGAACAGGC AGAAGTAGAA 
CAGGAGGAGG AAGATGACAA AAGCTTACTA ACACCATCTG CTAAAAGGGA CCTTGCAGAG 
GAAATAGAAA TGTACATGAA CAATATAAGC AGCCCCCTTG CAAGTCGAAC CCCTAGCATG 
GACCTTGACA GATCCAGTTC TGAACTAAAT AAAACATGTA AAGCTGTAGC ACTCAACAGG 
CGCTTGAGTG TTCCTGCTAG ATCCCCACAA GAACCAAGAC TAACCAGATC AATTACTGCC 
CACAGTAAAC GCGACAAAAG ACAAAGAAGC AGGCTCTGGT CTTCTCCTGC TGTTTCCGAG 
AACAATCCTC AGAATGAGCA AAGCCCAGAA TCTGCCCTTG CTTTAATGTC CCCAACATCA 
TTTAACATAG ATACACTTCT CACGCCAACC TTTGATGTTC TTAGGAGCAA CATGTTCTCA 
GCTGGCAAAG GAGTAGCCAA GAAGGCCAGC AAATGGTATT CAAAGATCGC AAATTATGCG 
GGGCCTGCAA AGGATCAGAA TTCTGACCGA GCCAGTCTGT CTTCAGGAGG AGCAGGGGAT 
ACTGAATCCT ACTCTTTAAA TGATGATGAT TTTGATGGCA GTGCTTCTCT GCATGAGACT 
GATGTCTTTC CTGGAACCAA TACCATTGGT GTAAGCAGAT GCAGCTTGGA GAGTAGCACA 
TCTCAAGACA GTAACTCTAA ACAACTTTAC AATAATGGTC TGCCATCAAC AGCATCTCCA 
AACTACAAGA GTGAGATTCT TTCATCCTGC AATTCCAGTA GCACTAGTGT CTTCCAGAAC 
TATGCTATGG AGGTTCTGAT ATCTAGCTGT TCACGTTGCT TGACCTGTGA CGGTCTTGTT 
CATGATGAGG AGATTATGGC AGGCTGGACG GCAGATGACT CAAACCTTAA CACCACATGT 
CCTTTTTGCG GGAACTCATT TCTTCCATCT TTAAACGTTG AAATTAGGGA CCTGCGACGC 
CCTGGAAGGT ACTTTCTGAA GGCCAGCCCA TCTGCAGAAA GTATGCCGCA TTCTTCAGTA 
TCCTCACAAA GACCTAACTC AGATATTCTG ACTCCGGCTT CTCCCTGTGC TTCCACCAAA 
GTCAAGGATT TTAGTTTGTG CAGTAAAACC AAACAAGAGA GAACTCGGTC TGTAACTGTT 
GAGATCCCAG TGGGGAAGAG AACCAGAGAT CTCAATGGGT CCTTATGCCC GAATCAGCCC 
ATTGCACGAA GTATCAGCAC ATTTGGTCCC TTAGATGAAC AGCCATCTCA TGACCAGAGC 
CATGTTATTG CCACTGGCAG CCTCCCAACT TGCTTCCAGG CACCTCCTGA GTCAGGATTG 
GAATGGAAGA TGTCAAATCC AGAGCCAATC ACAGTCCCTT ACCTTAGCCC GCTGGTTGTC 
TGGAAGGAGC TGGAGAGCCT GCTAGAAAAT GAGGGTGACC AATCGATTAC AGTTTCTGGA 
TTTGTGGACC ATCATCCGAT AGTATTCTGG AATTTGGTGT GGTATTTCAG GAGGCTGGAT 
CTCCCAAGTA ACCTCCCGGG ATTAATTCTG TCTTCGGAAC AATGCAATAA AAATTCAAAG 
GTCTCCAGGA GCTGTATGTC TGCAGACAGC AAACATGTCC TTATTCAAGT GCTGTGGGAC 
AATCTGAAAT TGCACCAGGA TCCCCGGCAG CCTCTCTACA TCCTCTGGAA CACTATTACA 
CAGGAACATG CGCTTGCTGT GCCACAGATC AAAGAGGAGG AATTGTTTGA TCAGCAGTTT 
CTCAGAGGGA TAGTAAGAAG CATAAAGAAC AACAATGTTT ATGGTCCTGT GAGCCAGATA 
TTGGACTGTA TTAAACAGGT CCCTGGAAGA ACGAGGCAGA GGAGTCTGTA CAGGGAAATT 
CTTTTTCTTT CTCTTGTTGC CTTGGGAAGA GAGAATATCG ATATTGATGT ATTTGATCGG 
GAATATAAAA CTGCATACGA GCGCCTCACC CCTGAGCAGC TGAACTGCAC ACAGATTTGT 
GATCGGCCTC CCAGCATTGG AGTGATGGAG TGCAGGAAGA CTTTCGGACA GCCATTCCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4a ( XM_031899252.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755112.1 Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X3, mRNA.

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CloneID	OXa55247
Clone ID Related Accession (Same CDS sequence)	XM_031899252.1
Accession Version	XM_031899252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	C-myc promoter-binding protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACTT TTGAGGAAGA GCAAGTAAGC AGTTGTCCTA CTCCAAAACG GAAGATGATG 
GAAGACAAAG GGCCCCGTGT GGCTGACTAC TTTGTAGTTA CAGGACTGAC AGATTCATCA 
AAACCACTTG AGGAGGAGAT CCACTTTAAT GATGCCTGTC ATAAAACAGC TAAACCAAAG 
GAACCCATTA CAGATGTGAC TGTGATAATC AAATCCTTAG GGGAGGAAGT GCCACAGGGA 
TATGTATGCA TAGACCTTAC CCCAACTGGA CTATCAGCCG ATCTCAATAA TGGAAGTATT 
CGAGGGCCAC AGATATATCT TTGTTACAGG AGAGGAAGAG ACAAACCACC TCTAACAGAT 
CTTGGGGTAT TGTACGAATG GAAGGAAAGA TTAAAACAGG GCTGCAAGAT TATTCAGGCT 
ACTCCATATG GCCGTCCAGC TAATATAAGC GGTAGTGCAT CCTCACAGAG AGTATTTATC 
ACATACCGCA GAGCTGCTGA GAGCATGACC CAGAATTCCT TGGCTGTAAC TGATATCTGC 
ATCATTATAC CAAGCAAAGG AGAGACTCCA CCACACACAT TTTGCAAAGT GGAAAAGAAT 
CTGAACAGCA GCGTGTGGGG CTCTGCTGTG TACCTGTGCT ACAAGAAATC AGTTGCCAAA 
ACAAATGCAG TATCCTATAA AGCTGGGTTG ATATGCAGGT ATCCAGAGGA GGATTATGAA 
TCATTTCCTC TGCCGGAATC AGTCCCACTA TTTTGTCTAC CCATGGGGGC AACAATTGAA 
TGTTGGCCAT CTGACAGTAA TTATCCACTC CCTGTTTTTT CCACTTTTGT TTTAACTGGA 
GCATCAGCTG AAAAGGTGTA TGGAGCTGCT ATTCAGTTTT ATGAGCCATA TGCTCAAGAT 
CAGCTGACTG AAAAGCAACA AGTACATTTA GGCCTCTTGA GCAGTGCAGA GGGTCAGATG 
GACTCTTCTA AAAGTATCCA TGTCAACAAG TGCATCTGCC TGCTGTCCCA CTGGCCTTTT 
TTTGATGCTT TCAAGAAATT TCTCACTTTC CTTTACCGCT ATTCAATATC GGGTCCTCAT 
GTGCTGCCAA TAGAAAAACA TATCTCCCAC TTCATGCACA AAGTCCCATT CCCATCTCCA 
CAGAGACCAC GCATTCTAGT TCAGCTTTCC CCGCATGATA CTCTGATCCT AAGCCAGCCT 
GTGTCCTCAC CACTTCCACT GAGTGGTGGA AAGTTTTCAA CGTTGCTGCA GAATTTGGGA 
CCTGAAAATG CTGTGACTCT GCTTGTATTT GCTGTCACGG AGCATAAGAT TCTGATCCAT 
TCGTTGCGCC CCTCGGTTCT CACAAGCGTC ACAGAAGCTT TAGTGTCTAT GATCTTCCCT 
TTCCATTGGC CGTGTCCTTA CATTCCACTT TGCCCTTTGG CCCTGGCAGA CGTACTAAGC 
GCCCCATGCC CCTTCATCGT GGGAATTGAT TCTCGCTACT TTGATCTGTA CGACCCCCCT 
CCGGATGTAA GCTGTGTTGA CCTAGATACC AACACCATCT CTCAAATTGA AGGCAAAAGA 
AATGTAACAT GGAAAATCCT ACCGAAGAAA GCTTGTAAGA ACCTGCTCAA CACTCTTTGT 
AACTTGTACC AGCAACTGGC AGAGTTGCAA CACAGACCAC GGGAAGAAGC TTTTATGGAA 
CTTGCAATGA ATGACTATGA CTTTAACTAT GGAAAAAGAA TGCATCAGCT GGACATGGAG 
ATTCAGGAAG CATTCCTGTG CTTTATGGCC TCTATTTTAA AAGGATACAG ATCGTATCTT 
CGGCCAATTA CCCAAGCACC ATCAGAGACT GCAACAGATG CCAGCTCTCT CTTCGAACTG 
CAAGGTTTCT TAAAAAGTCG TGATCGCTCA CACCACAAGT TTTACAACAT GATGACCAAG 
ACTCAGATGT TCATCCGCTT TATTGAGGAG TGTTCCTTCG TCAGCGATAA GGATGCTAGC 
CTGGCTTTCT TTGATGACTG TGTTGAGAAG GCGGATGTGG ATCGACCAGG AGAGACACGT 
CTTATTGAAC TGGATGAGTC TCACAGAAGT GAGCATACAG TGTTCATCAC ACCACCAGAG 
ATTCCCCACT TACCAAATGG CGAGGAGCCC CCTAAGAACT ATAGCTACAA TGGGTTCCCT 
GTCCTTATCT TGGATCTCTT TGACACGCCA GATGGACTCA TGAAAAGTCA CAAACTTAGT 
ACAAAAGTCA CCACACCCTG CAGTCCTTCT CCAATGTTTA GAAGAACAAA ACAGGAAATC 
CGGTCAGCAC ATAAAATAGC AAAGCAGTAC TCCTCCATCC CCCAGATGTG GTCCAGGTGT 
CTACTGAGAC ATTGCTATGG ACTTTGGTTT ATCTGTCTGC CCGCATATGT GAAATTCTGC 
CATTCCAAAG TGAGAGCACT GCGCACAGCC TACGATGTGT TGCGAAAAAT GCACATGAAA 
AAGATTGACT CTCCTGATGA GGTTTGCTAT AGGGTTCTAA TGCAGTTGTG TGGCCAATAT 
GGGCAGCCTG TCCTTGCTGT TAAAGTTCTC TTTGAAATGA GAAAGGTCGG CGTGCAGCCA 
AATGCTATAA CATATGGCTA TTATAATAAG GCTGTCCTGG AAAGCGTATG GCCCTCTGGG 
AATAGAGGGG GTTACTTTCT GTGGATAAAG GTGAGGAATG TGGTGCTAGC AGTAGCCCAG 
TTCAAACAGG CTTTGATAAA GCAACATGCT TCAGCTCATC AAACTCCTGC TTCAGATTGC 
AGTGATTTGG ACGCAGTCAG CCATGGCAGT TTGGATAGTT CTGTTGACAT GAACCCTACA 
GAACAGCCTT CATTCAGTAC AGATGTAACA TGTGCTGATC CAGCTGACGA CCGATCTAGT 
ACAGGAGGCC AGTCAGACCT TGGCTACAGT TCATTATCAA AAGAAGAAGT AAGAAGAGGA 
GCCGTGCATG ACATACAGGA GGAGAAGGAA GACTCCAGAG ATAGTGACTC CAGTTCGTCA 
TCTGATTCAG AAAGTTCCAA GAGCAGCAGA GACTGTGTGC CACAAGGAAC TGGCAGTCAG 
GGTCAACAAA CATTGGCAAG CACATCTAGT ATAGTCCGGA AAGAAGCAAA ATCAGACAGT 
GCAAGCAGTG ACAACTCAGC AGGTCTACTG TTCACACCAT CCTTTAGTGA GAATTGTGAA 
AGTGAAGTGT ATCAGTGCTG GAGTCTACAT AAAAGACACA AGAGTCCTGT GGAGTCAGAT 
CTGAAAAAAC AGATGGAATG GGAAAAGAGG AACAGGCATC TAAGCAGTGG CAATGTTGAG 
GAATTATTTA TCAGTAAGTC TGATGGACAG AATAACACTG AAAACATGGT GGACAAACTT 
GGAGCAGATG CAAAAATACT TAGCACTGTA TATAAGCAAC ACATGGCACG GCCCAAGACC 
CTGGACTTAA ATAAAATGAA TCCCAGAAAG TCAAAGGAGG TTGTTGAAAA AGAATCTAGT 
GATGATGAGT CTCCCATAGG AGGAGCAGAT GACAAAGTGG ACTCCCCTGT TATTTTTGAC 
CTTGAAGATC TAGATAATGA TTCTGATGAA CTTAAATCAG CAAAACCATG TGTCCGGAAG 
GCCAAACTTG CTCAAAGAAG TATCAGCATG CCCTTTTCAT CTTCCATGTT TCTGGCTAAG 
CCAGCTGAAA AAACAGACGT GAAGGCTGGA TTAGACCCTT TGTCATTGCT GGCAGCTGAA 
ACAGAACAGG CAGAAGTAGA ACAGGAGGAG GAAGATGACA AAAGCTTACT AACACCATCT 
GCTAAAAGGG ACCTTGCAGA GGAAATAGAA ATGTACATGA ACAATATAAG CAGCCCCCTT 
GCAAGTCGAA CCCCTAGCAT GGACCTTGAC AGATCCAGTT CTGAACTAAA TAAAACATGT 
AAAGCTGTAG CACTCAACAG GCGCTTGAGT GTTCCTGCTA GATCCCCACA AGAACCAAGA 
CTAACCAGAT CAATTACTGC CCACAGTAAA CGCGACAAAA GACAAAGAAG CAGGCTCTGG 
TCTTCTCCTG CTGTTTCCGA GAACAATCCT CAGAATGAGC AAAGCCCAGA ATCTGCCCTT 
GCTTTAATGT CCCCAACATC ATTTAACATA GATACACTTC TCACGCCAAC CTTTGATGTT 
CTTAGGAGCA ACATGTTCTC AGCTGGCAAA GGAGTAGCCA AGAAGGCCAG CAAATGGTAT 
TCAAAGATCG CAAATTATGC GGGGCCTGCA AAGGATCAGA ATTCTGACCG AGCCAGTCTG 
TCTTCAGGAG GAGCAGGGGA TACTGAATCC TACTCTTTAA ATGATGATGA TTTTGATGGC 
AGTGCTTCTC TGCATGAGAC TGATGTCTTT CCTGGAACCA ATACCATTGG TGTAAGCAGA 
TGCAGCTTGG AGAGTAGCAC ATCTCAAGAC AGTAACTCTA AACAACTTTA CAATAATGGT 
CTGCCATCAA CAGCATCTCC AAACTACAAG AGTGAGATTC TTTCATCCTG CAATTCCAGT 
AGCACTAGTG TCTTCCAGAA CTATGCTATG GAGGTTCTGA TATCTAGCTG TTCACGTTGC 
TTGACCTGTG ACGGTCTTGT TCATGATGAG GAGATTATGG CAGGCTGGAC GGCAGATGAC 
TCAAACCTTA ACACCACATG TCCTTTTTGC GGGAACTCAT TTCTTCCATC TTTAAACGTT 
GAAATTAGGG ACCTGCGACG CCCTGGAAGG TACTTTCTGA AGGCCAGCCC ATCTGCAGAA 
AGTATGCCGC ATTCTTCAGT ATCCTCACAA AGACCTAACT CAGATATTCT GACTCCGGCT 
TCTCCCTGTG CTTCCACCAA AGTCAAGGAT TTTAGTTTGT GCAGTAAAAC CAAACAAGAG 
AGAACTCGGT CTGTAACTGT TGAGATCCCA GTGGGGAAGA GAACCAGAGA TCTCAATGGG 
TCCTTATGCC CGAATCAGCC CATTGCACGA AGTATCAGCA CATTTGGTCC CTTAGATGAA 
CAGCCATCTC ATGACCAGAG CCATGTTATT GCCACTGGCA GCCTCCCAAC TTGCTTCCAG 
GCACCTCCTG AGTCAGGATT GGAATGGAAG ATGTCAAATC CAGAGCCAAT CACAGTCCCT 
TACCTTAGCC CGCTGGTTGT CTGGAAGGAG CTGGAGAGCC TGCTAGAAAA TGAGGGTGAC 
CAATCGATTA CAGTTTCTGG ATTTGTGGAC CATCATCCGA TAGTATTCTG GAATTTGGTG 
TGGTATTTCA GGAGGCTGGA TCTCCCAAGT AACCTCCCGG GATTAATTCT GTCTTCGGAA 
CAATGCAATA AAAATTCAAA GGTCTCCAGG AGCTGTATGT CTGCAGACAG CAAACATGTC 
CTTATTCAAG TGCTGTGGGA CAATCTGAAA TTGCACCAGG ATCCCCGGCA GCCTCTCTAC 
ATCCTCTGGA ACACTATTAG TCAAAACCGC ACTGTATTGT TTGATACACA GGAACATGCG 
CTTGCTGTGC CACAGATCAA AGAGGAGGAA TTGTTTGATC AGCAGTTTCT CAGAGGGATA 
GTAAGAAGCA TAAAGAACAA CAATGTTTAT GGTCCTGTGA GCCAGATATT GGACTGTATT 
AAACAGGTCC CTGGAAGAAC GAGGCAGAGG AGTCTGTACA GGGAAATTCT TTTTCTTTCT 
CTTGTTGCCT TGGGAAGAGA GAATATCGAT ATTGATGTAT TTGATCGGGA ATATAAAACT 
GCATACGAGC GCCTCACCCC TGAGCAGCTG AACTGCACAC AGATTTGTGA TCGGCCTCCC 
AGCATTGGAG TGATGGAGTG CAGGAAGACT TTCGGACAGC CATTCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755112.1
CDS	48..5858
Translation	MGTFEEEQVSSCPTPKRKMMEDKGPRVADYFVVTGLTDSSKPLEEEIHFNDACHKTAKPKEPITDVTVIIKSLGEEVPQGYVCIDLTPTGLSADLNNGSIRGPQIYLCYRRGRDKPPLTDLGVLYEWKERLKQGCKIIQATPYGRPANISGSASSQRVFITYRRAAESMTQNSLAVTDICIIIPSKGETPPHTFCKVEKNLNSSVWGSAVYLCYKKSVAKTNAVSYKAGLICRYPEEDYESFPLPESVPLFCLPMGATIECWPSDSNYPLPVFSTFVLTGASAEKVYGAAIQFYEPYAQDQLTEKQQVHLGLLSSAEGQMDSSKSIHVNKCICLLSHWPFFDAFKKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDTLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQIEGKRNVTWKILPKKACKNLLNTLCNLYQQLAELQHRPREEAFMELAMNDYDFNYGKRMHQLDMEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFELQGFLKSRDRSHHKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVEKADVDRPGETRLIELDESHRSEHTVFITPPEIPHLPNGEEPPKNYSYNGFPVLILDLFDTPDGLMKSHKLSTKVTTPCSPSPMFRRTKQEIRSAHKIAKQYSSIPQMWSRCLLRHCYGLWFICLPAYVKFCHSKVRALRTAYDVLRKMHMKKIDSPDEVCYRVLMQLCGQYGQPVLAVKVLFEMRKVGVQPNAITYGYYNKAVLESVWPSGNRGGYFLWIKVRNVVLAVAQFKQALIKQHASAHQTPASDCSDLDAVSHGSLDSSVDMNPTEQPSFSTDVTCADPADDRSSTGGQSDLGYSSLSKEEVRRGAVHDIQEEKEDSRDSDSSSSSDSESSKSSRDCVPQGTGSQGQQTLASTSSIVRKEAKSDSASSDNSAGLLFTPSFSENCESEVYQCWSLHKRHKSPVESDLKKQMEWEKRNRHLSSGNVEELFISKSDGQNNTENMVDKLGADAKILSTVYKQHMARPKTLDLNKMNPRKSKEVVEKESSDDESPIGGADDKVDSPVIFDLEDLDNDSDELKSAKPCVRKAKLAQRSISMPFSSSMFLAKPAEKTDVKAGLDPLSLLAAETEQAEVEQEEEDDKSLLTPSAKRDLAEEIEMYMNNISSPLASRTPSMDLDRSSSELNKTCKAVALNRRLSVPARSPQEPRLTRSITAHSKRDKRQRSRLWSSPAVSENNPQNEQSPESALALMSPTSFNIDTLLTPTFDVLRSNMFSAGKGVAKKASKWYSKIANYAGPAKDQNSDRASLSSGGAGDTESYSLNDDDFDGSASLHETDVFPGTNTIGVSRCSLESSTSQDSNSKQLYNNGLPSTASPNYKSEILSSCNSSSTSVFQNYAMEVLISSCSRCLTCDGLVHDEEIMAGWTADDSNLNTTCPFCGNSFLPSLNVEIRDLRRPGRYFLKASPSAESMPHSSVSSQRPNSDILTPASPCASTKVKDFSLCSKTKQERTRSVTVEIPVGKRTRDLNGSLCPNQPIARSISTFGPLDEQPSHDQSHVIATGSLPTCFQAPPESGLEWKMSNPEPITVPYLSPLVVWKELESLLENEGDQSITVSGFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEQCNKNSKVSRSCMSADSKHVLIQVLWDNLKLHQDPRQPLYILWNTISQNRTVLFDTQEHALAVPQIKEEELFDQQFLRGIVRSIKNNNVYGPVSQILDCIKQVPGRTRQRSLYREILFLSLVALGRENIDIDVFDREYKTAYERLTPEQLNCTQICDRPPSIGVMECRKTFGQPFL

Target ORF information:

RefSeq Version	XM_031899252.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899252.1

ORF Insert Sequence:

ATGGGAACTT TTGAGGAAGA GCAAGTAAGC AGTTGTCCTA CTCCAAAACG GAAGATGATG 
GAAGACAAAG GGCCCCGTGT GGCTGACTAC TTTGTAGTTA CAGGACTGAC AGATTCATCA 
AAACCACTTG AGGAGGAGAT CCACTTTAAT GATGCCTGTC ATAAAACAGC TAAACCAAAG 
GAACCCATTA CAGATGTGAC TGTGATAATC AAATCCTTAG GGGAGGAAGT GCCACAGGGA 
TATGTATGCA TAGACCTTAC CCCAACTGGA CTATCAGCCG ATCTCAATAA TGGAAGTATT 
CGAGGGCCAC AGATATATCT TTGTTACAGG AGAGGAAGAG ACAAACCACC TCTAACAGAT 
CTTGGGGTAT TGTACGAATG GAAGGAAAGA TTAAAACAGG GCTGCAAGAT TATTCAGGCT 
ACTCCATATG GCCGTCCAGC TAATATAAGC GGTAGTGCAT CCTCACAGAG AGTATTTATC 
ACATACCGCA GAGCTGCTGA GAGCATGACC CAGAATTCCT TGGCTGTAAC TGATATCTGC 
ATCATTATAC CAAGCAAAGG AGAGACTCCA CCACACACAT TTTGCAAAGT GGAAAAGAAT 
CTGAACAGCA GCGTGTGGGG CTCTGCTGTG TACCTGTGCT ACAAGAAATC AGTTGCCAAA 
ACAAATGCAG TATCCTATAA AGCTGGGTTG ATATGCAGGT ATCCAGAGGA GGATTATGAA 
TCATTTCCTC TGCCGGAATC AGTCCCACTA TTTTGTCTAC CCATGGGGGC AACAATTGAA 
TGTTGGCCAT CTGACAGTAA TTATCCACTC CCTGTTTTTT CCACTTTTGT TTTAACTGGA 
GCATCAGCTG AAAAGGTGTA TGGAGCTGCT ATTCAGTTTT ATGAGCCATA TGCTCAAGAT 
CAGCTGACTG AAAAGCAACA AGTACATTTA GGCCTCTTGA GCAGTGCAGA GGGTCAGATG 
GACTCTTCTA AAAGTATCCA TGTCAACAAG TGCATCTGCC TGCTGTCCCA CTGGCCTTTT 
TTTGATGCTT TCAAGAAATT TCTCACTTTC CTTTACCGCT ATTCAATATC GGGTCCTCAT 
GTGCTGCCAA TAGAAAAACA TATCTCCCAC TTCATGCACA AAGTCCCATT CCCATCTCCA 
CAGAGACCAC GCATTCTAGT TCAGCTTTCC CCGCATGATA CTCTGATCCT AAGCCAGCCT 
GTGTCCTCAC CACTTCCACT GAGTGGTGGA AAGTTTTCAA CGTTGCTGCA GAATTTGGGA 
CCTGAAAATG CTGTGACTCT GCTTGTATTT GCTGTCACGG AGCATAAGAT TCTGATCCAT 
TCGTTGCGCC CCTCGGTTCT CACAAGCGTC ACAGAAGCTT TAGTGTCTAT GATCTTCCCT 
TTCCATTGGC CGTGTCCTTA CATTCCACTT TGCCCTTTGG CCCTGGCAGA CGTACTAAGC 
GCCCCATGCC CCTTCATCGT GGGAATTGAT TCTCGCTACT TTGATCTGTA CGACCCCCCT 
CCGGATGTAA GCTGTGTTGA CCTAGATACC AACACCATCT CTCAAATTGA AGGCAAAAGA 
AATGTAACAT GGAAAATCCT ACCGAAGAAA GCTTGTAAGA ACCTGCTCAA CACTCTTTGT 
AACTTGTACC AGCAACTGGC AGAGTTGCAA CACAGACCAC GGGAAGAAGC TTTTATGGAA 
CTTGCAATGA ATGACTATGA CTTTAACTAT GGAAAAAGAA TGCATCAGCT GGACATGGAG 
ATTCAGGAAG CATTCCTGTG CTTTATGGCC TCTATTTTAA AAGGATACAG ATCGTATCTT 
CGGCCAATTA CCCAAGCACC ATCAGAGACT GCAACAGATG CCAGCTCTCT CTTCGAACTG 
CAAGGTTTCT TAAAAAGTCG TGATCGCTCA CACCACAAGT TTTACAACAT GATGACCAAG 
ACTCAGATGT TCATCCGCTT TATTGAGGAG TGTTCCTTCG TCAGCGATAA GGATGCTAGC 
CTGGCTTTCT TTGATGACTG TGTTGAGAAG GCGGATGTGG ATCGACCAGG AGAGACACGT 
CTTATTGAAC TGGATGAGTC TCACAGAAGT GAGCATACAG TGTTCATCAC ACCACCAGAG 
ATTCCCCACT TACCAAATGG CGAGGAGCCC CCTAAGAACT ATAGCTACAA TGGGTTCCCT 
GTCCTTATCT TGGATCTCTT TGACACGCCA GATGGACTCA TGAAAAGTCA CAAACTTAGT 
ACAAAAGTCA CCACACCCTG CAGTCCTTCT CCAATGTTTA GAAGAACAAA ACAGGAAATC 
CGGTCAGCAC ATAAAATAGC AAAGCAGTAC TCCTCCATCC CCCAGATGTG GTCCAGGTGT 
CTACTGAGAC ATTGCTATGG ACTTTGGTTT ATCTGTCTGC CCGCATATGT GAAATTCTGC 
CATTCCAAAG TGAGAGCACT GCGCACAGCC TACGATGTGT TGCGAAAAAT GCACATGAAA 
AAGATTGACT CTCCTGATGA GGTTTGCTAT AGGGTTCTAA TGCAGTTGTG TGGCCAATAT 
GGGCAGCCTG TCCTTGCTGT TAAAGTTCTC TTTGAAATGA GAAAGGTCGG CGTGCAGCCA 
AATGCTATAA CATATGGCTA TTATAATAAG GCTGTCCTGG AAAGCGTATG GCCCTCTGGG 
AATAGAGGGG GTTACTTTCT GTGGATAAAG GTGAGGAATG TGGTGCTAGC AGTAGCCCAG 
TTCAAACAGG CTTTGATAAA GCAACATGCT TCAGCTCATC AAACTCCTGC TTCAGATTGC 
AGTGATTTGG ACGCAGTCAG CCATGGCAGT TTGGATAGTT CTGTTGACAT GAACCCTACA 
GAACAGCCTT CATTCAGTAC AGATGTAACA TGTGCTGATC CAGCTGACGA CCGATCTAGT 
ACAGGAGGCC AGTCAGACCT TGGCTACAGT TCATTATCAA AAGAAGAAGT AAGAAGAGGA 
GCCGTGCATG ACATACAGGA GGAGAAGGAA GACTCCAGAG ATAGTGACTC CAGTTCGTCA 
TCTGATTCAG AAAGTTCCAA GAGCAGCAGA GACTGTGTGC CACAAGGAAC TGGCAGTCAG 
GGTCAACAAA CATTGGCAAG CACATCTAGT ATAGTCCGGA AAGAAGCAAA ATCAGACAGT 
GCAAGCAGTG ACAACTCAGC AGGTCTACTG TTCACACCAT CCTTTAGTGA GAATTGTGAA 
AGTGAAGTGT ATCAGTGCTG GAGTCTACAT AAAAGACACA AGAGTCCTGT GGAGTCAGAT 
CTGAAAAAAC AGATGGAATG GGAAAAGAGG AACAGGCATC TAAGCAGTGG CAATGTTGAG 
GAATTATTTA TCAGTAAGTC TGATGGACAG AATAACACTG AAAACATGGT GGACAAACTT 
GGAGCAGATG CAAAAATACT TAGCACTGTA TATAAGCAAC ACATGGCACG GCCCAAGACC 
CTGGACTTAA ATAAAATGAA TCCCAGAAAG TCAAAGGAGG TTGTTGAAAA AGAATCTAGT 
GATGATGAGT CTCCCATAGG AGGAGCAGAT GACAAAGTGG ACTCCCCTGT TATTTTTGAC 
CTTGAAGATC TAGATAATGA TTCTGATGAA CTTAAATCAG CAAAACCATG TGTCCGGAAG 
GCCAAACTTG CTCAAAGAAG TATCAGCATG CCCTTTTCAT CTTCCATGTT TCTGGCTAAG 
CCAGCTGAAA AAACAGACGT GAAGGCTGGA TTAGACCCTT TGTCATTGCT GGCAGCTGAA 
ACAGAACAGG CAGAAGTAGA ACAGGAGGAG GAAGATGACA AAAGCTTACT AACACCATCT 
GCTAAAAGGG ACCTTGCAGA GGAAATAGAA ATGTACATGA ACAATATAAG CAGCCCCCTT 
GCAAGTCGAA CCCCTAGCAT GGACCTTGAC AGATCCAGTT CTGAACTAAA TAAAACATGT 
AAAGCTGTAG CACTCAACAG GCGCTTGAGT GTTCCTGCTA GATCCCCACA AGAACCAAGA 
CTAACCAGAT CAATTACTGC CCACAGTAAA CGCGACAAAA GACAAAGAAG CAGGCTCTGG 
TCTTCTCCTG CTGTTTCCGA GAACAATCCT CAGAATGAGC AAAGCCCAGA ATCTGCCCTT 
GCTTTAATGT CCCCAACATC ATTTAACATA GATACACTTC TCACGCCAAC CTTTGATGTT 
CTTAGGAGCA ACATGTTCTC AGCTGGCAAA GGAGTAGCCA AGAAGGCCAG CAAATGGTAT 
TCAAAGATCG CAAATTATGC GGGGCCTGCA AAGGATCAGA ATTCTGACCG AGCCAGTCTG 
TCTTCAGGAG GAGCAGGGGA TACTGAATCC TACTCTTTAA ATGATGATGA TTTTGATGGC 
AGTGCTTCTC TGCATGAGAC TGATGTCTTT CCTGGAACCA ATACCATTGG TGTAAGCAGA 
TGCAGCTTGG AGAGTAGCAC ATCTCAAGAC AGTAACTCTA AACAACTTTA CAATAATGGT 
CTGCCATCAA CAGCATCTCC AAACTACAAG AGTGAGATTC TTTCATCCTG CAATTCCAGT 
AGCACTAGTG TCTTCCAGAA CTATGCTATG GAGGTTCTGA TATCTAGCTG TTCACGTTGC 
TTGACCTGTG ACGGTCTTGT TCATGATGAG GAGATTATGG CAGGCTGGAC GGCAGATGAC 
TCAAACCTTA ACACCACATG TCCTTTTTGC GGGAACTCAT TTCTTCCATC TTTAAACGTT 
GAAATTAGGG ACCTGCGACG CCCTGGAAGG TACTTTCTGA AGGCCAGCCC ATCTGCAGAA 
AGTATGCCGC ATTCTTCAGT ATCCTCACAA AGACCTAACT CAGATATTCT GACTCCGGCT 
TCTCCCTGTG CTTCCACCAA AGTCAAGGAT TTTAGTTTGT GCAGTAAAAC CAAACAAGAG 
AGAACTCGGT CTGTAACTGT TGAGATCCCA GTGGGGAAGA GAACCAGAGA TCTCAATGGG 
TCCTTATGCC CGAATCAGCC CATTGCACGA AGTATCAGCA CATTTGGTCC CTTAGATGAA 
CAGCCATCTC ATGACCAGAG CCATGTTATT GCCACTGGCA GCCTCCCAAC TTGCTTCCAG 
GCACCTCCTG AGTCAGGATT GGAATGGAAG ATGTCAAATC CAGAGCCAAT CACAGTCCCT 
TACCTTAGCC CGCTGGTTGT CTGGAAGGAG CTGGAGAGCC TGCTAGAAAA TGAGGGTGAC 
CAATCGATTA CAGTTTCTGG ATTTGTGGAC CATCATCCGA TAGTATTCTG GAATTTGGTG 
TGGTATTTCA GGAGGCTGGA TCTCCCAAGT AACCTCCCGG GATTAATTCT GTCTTCGGAA 
CAATGCAATA AAAATTCAAA GGTCTCCAGG AGCTGTATGT CTGCAGACAG CAAACATGTC 
CTTATTCAAG TGCTGTGGGA CAATCTGAAA TTGCACCAGG ATCCCCGGCA GCCTCTCTAC 
ATCCTCTGGA ACACTATTAG TCAAAACCGC ACTGTATTGT TTGATACACA GGAACATGCG 
CTTGCTGTGC CACAGATCAA AGAGGAGGAA TTGTTTGATC AGCAGTTTCT CAGAGGGATA 
GTAAGAAGCA TAAAGAACAA CAATGTTTAT GGTCCTGTGA GCCAGATATT GGACTGTATT 
AAACAGGTCC CTGGAAGAAC GAGGCAGAGG AGTCTGTACA GGGAAATTCT TTTTCTTTCT 
CTTGTTGCCT TGGGAAGAGA GAATATCGAT ATTGATGTAT TTGATCGGGA ATATAAAACT 
GCATACGAGC GCCTCACCCC TGAGCAGCTG AACTGCACAC AGATTTGTGA TCGGCCTCCC 
AGCATTGGAG TGATGGAGTG CAGGAAGACT TTCGGACAGC CATTCCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4a ( XM_031899254.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755114.1 Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X5, mRNA.

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CloneID	OXa55248
Clone ID Related Accession (Same CDS sequence)	XM_031899254.1 , XM_031899253.1
Accession Version	XM_031899254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	C-myc promoter-binding protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCCGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGCTTT TCTCAGAGAG GAATAATGCA 
GATAAAGGTG GCAAGGCGGA TGTGGATCGA CCAGGAGAGA CACGTCTTAT TGAACTGGAT 
GAGTCTCACA GAAGTGAGCA TACAGTGTTC ATCACACCAC CAGAGATTCC CCACTTACCA 
AATGGCGAGG AGCCCCCTAA GAACTATAGC TACAATGGGT TCCCTGTCCT TATCTTGGAT 
CTCTTTGACA CGCCAGATGG ACTCATGAAA AGTCACAAAC TTAGTACAAA AGTCACCACA 
CCCTGCAGTC CTTCTCCAAT GTTTAGAAGA ACAAAACAGG AAATCCGGTC AGCACATAAA 
ATAGCAAAGC AGTACTCCTC CATCCCCCAG ATGTGGTCCA GGTGTCTACT GAGACATTGC 
TATGGACTTT GGTTTATCTG TCTGCCCGCA TATGTGAAAT TCTGCCATTC CAAAGTGAGA 
GCACTGCGCA CAGCCTACGA TGTGTTGCGA AAAATGCACA TGAAAAAGAT TGACTCTCCT 
GATGAGGTTT GCTATAGGGT TCTAATGCAG TTGTGTGGCC AATATGGGCA GCCTGTCCTT 
GCTGTTAAAG TTCTCTTTGA AATGAGAAAG GTCGGCGTGC AGCCAAATGC TATAACATAT 
GGCTATTATA ATAAGGCTGT CCTGGAAAGC GTATGGCCCT CTGGGAATAG AGGGGGTTAC 
TTTCTGTGGA TAAAGGTGAG GAATGTGGTG CTAGCAGTAG CCCAGTTCAA ACAGGCTTTG 
ATAAAGCAAC ATGCTTCAGC TCATCAAACT CCTGCTTCAG ATTGCAGTGA TTTGGACGCA 
GTCAGCCATG GCAGTTTGGA TAGTTCTGTT GACATGAACC CTACAGAACA GCCTTCATTC 
AGTACAGATG TAACATGTGC TGATCCAGCT GACGACCGAT CTAGTACAGG AGGCCAGTCA 
GACCTTGGCT ACAGTTCATT ATCAAAAGAA GAAGTAAGAA GAGGAGCCGT GCATGACATA 
CAGGAGGAGA AGGAAGACTC CAGAGATAGT GACTCCAGTT CGTCATCTGA TTCAGAAAGT 
TCCAAGAGCA GCAGAGACTG TGTGCCACAA GGAACTGGCA GTCAGGGTCA ACAAACATTG 
GCAAGCACAT CTAGTATAGT CCGGAAAGAA GCAAAATCAG ACAGTGCAAG CAGTGACAAC 
TCAGCAGGTC TACTGTTCAC ACCATCCTTT AGTGAGAATT GTGAAAGTGA AGTGTATCAG 
TGCTGGAGTC TACATAAAAG ACACAAGAGT CCTGTGGAGT CAGATCTGAA AAAACAGATG 
GAATGGGAAA AGAGGAACAG GCATCTAAGC AGTGGCAATG TTGAGGAATT ATTTATCAGT 
AAGTCTGATG GACAGAATAA CACTGAAAAC ATGGTGGACA AACTTGGAGC AGATGCAAAA 
ATACTTAGCA CTGTATATAA GCAACACATG GCACGGCCCA AGACCCTGGA CTTAAATAAA 
ATGAATCCCA GAAAGTCAAA GGAGGTTGTT GAAAAAGAAT CTAGTGATGA TGAGTCTCCC 
ATAGGAGGAG CAGATGACAA AGTGGACTCC CCTGTTATTT TTGACCTTGA AGATCTAGAT 
AATGATTCTG ATGAACTTAA ATCAGCAAAA CCATGTGTCC GGAAGGCCAA ACTTGCTCAA 
AGAAGTATCA GCATGCCCTT TTCATCTTCC ATGTTTCTGG CTAAGCCAGC TGAAAAAACA 
GACGTGAAGG CTGGATTAGA CCCTTTGTCA TTGCTGGCAG CTGAAACAGA ACAGGCAGAA 
GTAGAACAGG AGGAGGAAGA TGACAAAAGC TTACTAACAC CATCTGCTAA AAGGGACCTT 
GCAGAGGAAA TAGAAATGTA CATGAACAAT ATAAGCAGCC CCCTTGCAAG TCGAACCCCT 
AGCATGGACC TTGACAGATC CAGTTCTGAA CTAAATAAAA CATGTAAAGC TGTAGCACTC 
AACAGGCGCT TGAGTGTTCC TGCTAGATCC CCACAAGAAC CAAGACTAAC CAGATCAATT 
ACTGCCCACA GTAAACGCGA CAAAAGACAA AGAAGCAGGC TCTGGTCTTC TCCTGCTGTT 
TCCGAGAACA ATCCTCAGAA TGAGCAAAGC CCAGAATCTG CCCTTGCTTT AATGTCCCCA 
ACATCATTTA ACATAGATAC ACTTCTCACG CCAACCTTTG ATGTTCTTAG GAGCAACATG 
TTCTCAGCTG GCAAAGGAGT AGCCAAGAAG GCCAGCAAAT GGTATTCAAA GATCGCAAAT 
TATGCGGGGC CTGCAAAGGA TCAGAATTCT GACCGAGCCA GTCTGTCTTC AGGAGGAGCA 
GGGGATACTG AATCCTACTC TTTAAATGAT GATGATTTTG ATGGCAGTGC TTCTCTGCAT 
GAGACTGATG TCTTTCCTGG AACCAATACC ATTGGTGTAA GCAGATGCAG CTTGGAGAGT 
AGCACATCTC AAGACAGTAA CTCTAAACAA CTTTACAATA ATGGTCTGCC ATCAACAGCA 
TCTCCAAACT ACAAGAGTGA GATTCTTTCA TCCTGCAATT CCAGTAGCAC TAGTGTCTTC 
CAGAACTATG CTATGGAGGT TCTGATATCT AGCTGTTCAC GTTGCTTGAC CTGTGACGGT 
CTTGTTCATG ATGAGGAGAT TATGGCAGGC TGGACGGCAG ATGACTCAAA CCTTAACACC 
ACATGTCCTT TTTGCGGGAA CTCATTTCTT CCATCTTTAA ACGTTGAAAT TAGGGACCTG 
CGACGCCCTG GAAGGTACTT TCTGAAGGCC AGCCCATCTG CAGAAAGTAT GCCGCATTCT 
TCAGTATCCT CACAAAGACC TAACTCAGAT ATTCTGACTC CGGCTTCTCC CTGTGCTTCC 
ACCAAAGTCA AGGATTTTAG TTTGTGCAGT AAAACCAAAC AAGAGAGAAC TCGGTCTGTA 
ACTGTTGAGA TCCCAGTGGG GAAGAGAACC AGAGATCTCA ATGGGTCCTT ATGCCCGAAT 
CAGCCCATTG CACGAAGTAT CAGCACATTT GGTCCCTTAG ATGAACAGCC ATCTCATGAC 
CAGAGCCATG TTATTGCCAC TGGCAGCCTC CCAACTTGCT TCCAGGCACC TCCTGAGTCA 
GGATTGGAAT GGAAGATGTC AAATCCAGAG CCAATCACAG TCCCTTACCT TAGCCCGCTG 
GTTGTCTGGA AGGAGCTGGA GAGCCTGCTA GAAAATGAGG GTGACCAATC GATTACAGTT 
TCTGGATTTG TGGACCATCA TCCGATAGTA TTCTGGAATT TGGTGTGGTA TTTCAGGAGG 
CTGGATCTCC CAAGTAACCT CCCGGGATTA ATTCTGTCTT CGGAACAATG CAATAAAAAT 
TCAAAGGTCT CCAGGAGCTG TATGTCTGCA GACAGCAAAC ATGTCCTTAT TCAAGTGCTG 
TGGGACAATC TGAAATTGCA CCAGGATCCC CGGCAGCCTC TCTACATCCT CTGGAACACT 
ATTAGTCAAA ACCGCACTGT ATTGTTTGAT ACACAGGAAC ATGCGCTTGC TGTGCCACAG 
ATCAAAGAGG AGGAATTGTT TGATCAGCAG TTTCTCAGAG GGATAGTAAG AAGCATAAAG 
AACAACAATG TTTATGGTCC TGTGAGCCAG ATATTGGACT GTATTAAACA GGTCCCTGGA 
AGAACGAGGC AGAGGAGTCT GTACAGGGAA ATTCTTTTTC TTTCTCTTGT TGCCTTGGGA 
AGAGAGAATA TCGATATTGA TGTATTTGAT CGGGAATATA AAACTGCATA CGAGCGCCTC 
ACCCCTGAGC AGCTGAACTG CACACAGATT TGTGATCGGC CTCCCAGCAT TGGAGTGATG 
GAGTGCAGGA AGACTTTCGG ACAGCCATTC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755114.1
CDS	139..5934
Translation	MMEDKGPRVADYFVVTGLTDSSKPLEEEIHFNDACHKTAKPKEPITDVTVIIKSLGEEVPQGYVCIDLTPTGLSADLNNGSIRGPQIYLCYRRGRDKPPLTDLGVLYEWKERLKQGCKIIQATPYGRPANISGSASSQRVFITYRRAAESMTQNSLAVTDICIIIPSKGETPPHTFCKVEKNLNSSVWGSAVYLCYKKSVAKTNAVSYKAGLICRYPEEDYESFPLPESVPLFCLPMGATIECWPSDSNYPLPVFSTFVLTGASAEKVYGAAIQFYEPYAQDQLTEKQQVHLGLLSSAEGQMDSSKSIHVNKCICLLSHWPFFDAFKKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDTLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQIEGKRNVTWKILPKKACKNLLNTLCNLYQQLAELQHRPREEAFMELAMNDYDFNYGKRMHQLDMEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFELQGFLKSRDRSHHKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVEKLFSERNNADKGGKADVDRPGETRLIELDESHRSEHTVFITPPEIPHLPNGEEPPKNYSYNGFPVLILDLFDTPDGLMKSHKLSTKVTTPCSPSPMFRRTKQEIRSAHKIAKQYSSIPQMWSRCLLRHCYGLWFICLPAYVKFCHSKVRALRTAYDVLRKMHMKKIDSPDEVCYRVLMQLCGQYGQPVLAVKVLFEMRKVGVQPNAITYGYYNKAVLESVWPSGNRGGYFLWIKVRNVVLAVAQFKQALIKQHASAHQTPASDCSDLDAVSHGSLDSSVDMNPTEQPSFSTDVTCADPADDRSSTGGQSDLGYSSLSKEEVRRGAVHDIQEEKEDSRDSDSSSSSDSESSKSSRDCVPQGTGSQGQQTLASTSSIVRKEAKSDSASSDNSAGLLFTPSFSENCESEVYQCWSLHKRHKSPVESDLKKQMEWEKRNRHLSSGNVEELFISKSDGQNNTENMVDKLGADAKILSTVYKQHMARPKTLDLNKMNPRKSKEVVEKESSDDESPIGGADDKVDSPVIFDLEDLDNDSDELKSAKPCVRKAKLAQRSISMPFSSSMFLAKPAEKTDVKAGLDPLSLLAAETEQAEVEQEEEDDKSLLTPSAKRDLAEEIEMYMNNISSPLASRTPSMDLDRSSSELNKTCKAVALNRRLSVPARSPQEPRLTRSITAHSKRDKRQRSRLWSSPAVSENNPQNEQSPESALALMSPTSFNIDTLLTPTFDVLRSNMFSAGKGVAKKASKWYSKIANYAGPAKDQNSDRASLSSGGAGDTESYSLNDDDFDGSASLHETDVFPGTNTIGVSRCSLESSTSQDSNSKQLYNNGLPSTASPNYKSEILSSCNSSSTSVFQNYAMEVLISSCSRCLTCDGLVHDEEIMAGWTADDSNLNTTCPFCGNSFLPSLNVEIRDLRRPGRYFLKASPSAESMPHSSVSSQRPNSDILTPASPCASTKVKDFSLCSKTKQERTRSVTVEIPVGKRTRDLNGSLCPNQPIARSISTFGPLDEQPSHDQSHVIATGSLPTCFQAPPESGLEWKMSNPEPITVPYLSPLVVWKELESLLENEGDQSITVSGFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEQCNKNSKVSRSCMSADSKHVLIQVLWDNLKLHQDPRQPLYILWNTISQNRTVLFDTQEHALAVPQIKEEELFDQQFLRGIVRSIKNNNVYGPVSQILDCIKQVPGRTRQRSLYREILFLSLVALGRENIDIDVFDREYKTAYERLTPEQLNCTQICDRPPSIGVMECRKTFGQPFL

Target ORF information:

RefSeq Version	XM_031899254.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899254.1

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCCGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGCTTT TCTCAGAGAG GAATAATGCA 
GATAAAGGTG GCAAGGCGGA TGTGGATCGA CCAGGAGAGA CACGTCTTAT TGAACTGGAT 
GAGTCTCACA GAAGTGAGCA TACAGTGTTC ATCACACCAC CAGAGATTCC CCACTTACCA 
AATGGCGAGG AGCCCCCTAA GAACTATAGC TACAATGGGT TCCCTGTCCT TATCTTGGAT 
CTCTTTGACA CGCCAGATGG ACTCATGAAA AGTCACAAAC TTAGTACAAA AGTCACCACA 
CCCTGCAGTC CTTCTCCAAT GTTTAGAAGA ACAAAACAGG AAATCCGGTC AGCACATAAA 
ATAGCAAAGC AGTACTCCTC CATCCCCCAG ATGTGGTCCA GGTGTCTACT GAGACATTGC 
TATGGACTTT GGTTTATCTG TCTGCCCGCA TATGTGAAAT TCTGCCATTC CAAAGTGAGA 
GCACTGCGCA CAGCCTACGA TGTGTTGCGA AAAATGCACA TGAAAAAGAT TGACTCTCCT 
GATGAGGTTT GCTATAGGGT TCTAATGCAG TTGTGTGGCC AATATGGGCA GCCTGTCCTT 
GCTGTTAAAG TTCTCTTTGA AATGAGAAAG GTCGGCGTGC AGCCAAATGC TATAACATAT 
GGCTATTATA ATAAGGCTGT CCTGGAAAGC GTATGGCCCT CTGGGAATAG AGGGGGTTAC 
TTTCTGTGGA TAAAGGTGAG GAATGTGGTG CTAGCAGTAG CCCAGTTCAA ACAGGCTTTG 
ATAAAGCAAC ATGCTTCAGC TCATCAAACT CCTGCTTCAG ATTGCAGTGA TTTGGACGCA 
GTCAGCCATG GCAGTTTGGA TAGTTCTGTT GACATGAACC CTACAGAACA GCCTTCATTC 
AGTACAGATG TAACATGTGC TGATCCAGCT GACGACCGAT CTAGTACAGG AGGCCAGTCA 
GACCTTGGCT ACAGTTCATT ATCAAAAGAA GAAGTAAGAA GAGGAGCCGT GCATGACATA 
CAGGAGGAGA AGGAAGACTC CAGAGATAGT GACTCCAGTT CGTCATCTGA TTCAGAAAGT 
TCCAAGAGCA GCAGAGACTG TGTGCCACAA GGAACTGGCA GTCAGGGTCA ACAAACATTG 
GCAAGCACAT CTAGTATAGT CCGGAAAGAA GCAAAATCAG ACAGTGCAAG CAGTGACAAC 
TCAGCAGGTC TACTGTTCAC ACCATCCTTT AGTGAGAATT GTGAAAGTGA AGTGTATCAG 
TGCTGGAGTC TACATAAAAG ACACAAGAGT CCTGTGGAGT CAGATCTGAA AAAACAGATG 
GAATGGGAAA AGAGGAACAG GCATCTAAGC AGTGGCAATG TTGAGGAATT ATTTATCAGT 
AAGTCTGATG GACAGAATAA CACTGAAAAC ATGGTGGACA AACTTGGAGC AGATGCAAAA 
ATACTTAGCA CTGTATATAA GCAACACATG GCACGGCCCA AGACCCTGGA CTTAAATAAA 
ATGAATCCCA GAAAGTCAAA GGAGGTTGTT GAAAAAGAAT CTAGTGATGA TGAGTCTCCC 
ATAGGAGGAG CAGATGACAA AGTGGACTCC CCTGTTATTT TTGACCTTGA AGATCTAGAT 
AATGATTCTG ATGAACTTAA ATCAGCAAAA CCATGTGTCC GGAAGGCCAA ACTTGCTCAA 
AGAAGTATCA GCATGCCCTT TTCATCTTCC ATGTTTCTGG CTAAGCCAGC TGAAAAAACA 
GACGTGAAGG CTGGATTAGA CCCTTTGTCA TTGCTGGCAG CTGAAACAGA ACAGGCAGAA 
GTAGAACAGG AGGAGGAAGA TGACAAAAGC TTACTAACAC CATCTGCTAA AAGGGACCTT 
GCAGAGGAAA TAGAAATGTA CATGAACAAT ATAAGCAGCC CCCTTGCAAG TCGAACCCCT 
AGCATGGACC TTGACAGATC CAGTTCTGAA CTAAATAAAA CATGTAAAGC TGTAGCACTC 
AACAGGCGCT TGAGTGTTCC TGCTAGATCC CCACAAGAAC CAAGACTAAC CAGATCAATT 
ACTGCCCACA GTAAACGCGA CAAAAGACAA AGAAGCAGGC TCTGGTCTTC TCCTGCTGTT 
TCCGAGAACA ATCCTCAGAA TGAGCAAAGC CCAGAATCTG CCCTTGCTTT AATGTCCCCA 
ACATCATTTA ACATAGATAC ACTTCTCACG CCAACCTTTG ATGTTCTTAG GAGCAACATG 
TTCTCAGCTG GCAAAGGAGT AGCCAAGAAG GCCAGCAAAT GGTATTCAAA GATCGCAAAT 
TATGCGGGGC CTGCAAAGGA TCAGAATTCT GACCGAGCCA GTCTGTCTTC AGGAGGAGCA 
GGGGATACTG AATCCTACTC TTTAAATGAT GATGATTTTG ATGGCAGTGC TTCTCTGCAT 
GAGACTGATG TCTTTCCTGG AACCAATACC ATTGGTGTAA GCAGATGCAG CTTGGAGAGT 
AGCACATCTC AAGACAGTAA CTCTAAACAA CTTTACAATA ATGGTCTGCC ATCAACAGCA 
TCTCCAAACT ACAAGAGTGA GATTCTTTCA TCCTGCAATT CCAGTAGCAC TAGTGTCTTC 
CAGAACTATG CTATGGAGGT TCTGATATCT AGCTGTTCAC GTTGCTTGAC CTGTGACGGT 
CTTGTTCATG ATGAGGAGAT TATGGCAGGC TGGACGGCAG ATGACTCAAA CCTTAACACC 
ACATGTCCTT TTTGCGGGAA CTCATTTCTT CCATCTTTAA ACGTTGAAAT TAGGGACCTG 
CGACGCCCTG GAAGGTACTT TCTGAAGGCC AGCCCATCTG CAGAAAGTAT GCCGCATTCT 
TCAGTATCCT CACAAAGACC TAACTCAGAT ATTCTGACTC CGGCTTCTCC CTGTGCTTCC 
ACCAAAGTCA AGGATTTTAG TTTGTGCAGT AAAACCAAAC AAGAGAGAAC TCGGTCTGTA 
ACTGTTGAGA TCCCAGTGGG GAAGAGAACC AGAGATCTCA ATGGGTCCTT ATGCCCGAAT 
CAGCCCATTG CACGAAGTAT CAGCACATTT GGTCCCTTAG ATGAACAGCC ATCTCATGAC 
CAGAGCCATG TTATTGCCAC TGGCAGCCTC CCAACTTGCT TCCAGGCACC TCCTGAGTCA 
GGATTGGAAT GGAAGATGTC AAATCCAGAG CCAATCACAG TCCCTTACCT TAGCCCGCTG 
GTTGTCTGGA AGGAGCTGGA GAGCCTGCTA GAAAATGAGG GTGACCAATC GATTACAGTT 
TCTGGATTTG TGGACCATCA TCCGATAGTA TTCTGGAATT TGGTGTGGTA TTTCAGGAGG 
CTGGATCTCC CAAGTAACCT CCCGGGATTA ATTCTGTCTT CGGAACAATG CAATAAAAAT 
TCAAAGGTCT CCAGGAGCTG TATGTCTGCA GACAGCAAAC ATGTCCTTAT TCAAGTGCTG 
TGGGACAATC TGAAATTGCA CCAGGATCCC CGGCAGCCTC TCTACATCCT CTGGAACACT 
ATTAGTCAAA ACCGCACTGT ATTGTTTGAT ACACAGGAAC ATGCGCTTGC TGTGCCACAG 
ATCAAAGAGG AGGAATTGTT TGATCAGCAG TTTCTCAGAG GGATAGTAAG AAGCATAAAG 
AACAACAATG TTTATGGTCC TGTGAGCCAG ATATTGGACT GTATTAAACA GGTCCCTGGA 
AGAACGAGGC AGAGGAGTCT GTACAGGGAA ATTCTTTTTC TTTCTCTTGT TGCCTTGGGA 
AGAGAGAATA TCGATATTGA TGTATTTGAT CGGGAATATA AAACTGCATA CGAGCGCCTC 
ACCCCTGAGC AGCTGAACTG CACACAGATT TGTGATCGGC CTCCCAGCAT TGGAGTGATG 
GAGTGCAGGA AGACTTTCGG ACAGCCATTC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4a ( XM_031899253.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755113.1 Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X4, mRNA.

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CloneID	OXa55248
Clone ID Related Accession (Same CDS sequence)	XM_031899254.1 , XM_031899253.1
Accession Version	XM_031899253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	C-myc promoter-binding protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCCGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGCTTT TCTCAGAGAG GAATAATGCA 
GATAAAGGTG GCAAGGCGGA TGTGGATCGA CCAGGAGAGA CACGTCTTAT TGAACTGGAT 
GAGTCTCACA GAAGTGAGCA TACAGTGTTC ATCACACCAC CAGAGATTCC CCACTTACCA 
AATGGCGAGG AGCCCCCTAA GAACTATAGC TACAATGGGT TCCCTGTCCT TATCTTGGAT 
CTCTTTGACA CGCCAGATGG ACTCATGAAA AGTCACAAAC TTAGTACAAA AGTCACCACA 
CCCTGCAGTC CTTCTCCAAT GTTTAGAAGA ACAAAACAGG AAATCCGGTC AGCACATAAA 
ATAGCAAAGC AGTACTCCTC CATCCCCCAG ATGTGGTCCA GGTGTCTACT GAGACATTGC 
TATGGACTTT GGTTTATCTG TCTGCCCGCA TATGTGAAAT TCTGCCATTC CAAAGTGAGA 
GCACTGCGCA CAGCCTACGA TGTGTTGCGA AAAATGCACA TGAAAAAGAT TGACTCTCCT 
GATGAGGTTT GCTATAGGGT TCTAATGCAG TTGTGTGGCC AATATGGGCA GCCTGTCCTT 
GCTGTTAAAG TTCTCTTTGA AATGAGAAAG GTCGGCGTGC AGCCAAATGC TATAACATAT 
GGCTATTATA ATAAGGCTGT CCTGGAAAGC GTATGGCCCT CTGGGAATAG AGGGGGTTAC 
TTTCTGTGGA TAAAGGTGAG GAATGTGGTG CTAGCAGTAG CCCAGTTCAA ACAGGCTTTG 
ATAAAGCAAC ATGCTTCAGC TCATCAAACT CCTGCTTCAG ATTGCAGTGA TTTGGACGCA 
GTCAGCCATG GCAGTTTGGA TAGTTCTGTT GACATGAACC CTACAGAACA GCCTTCATTC 
AGTACAGATG TAACATGTGC TGATCCAGCT GACGACCGAT CTAGTACAGG AGGCCAGTCA 
GACCTTGGCT ACAGTTCATT ATCAAAAGAA GAAGTAAGAA GAGGAGCCGT GCATGACATA 
CAGGAGGAGA AGGAAGACTC CAGAGATAGT GACTCCAGTT CGTCATCTGA TTCAGAAAGT 
TCCAAGAGCA GCAGAGACTG TGTGCCACAA GGAACTGGCA GTCAGGGTCA ACAAACATTG 
GCAAGCACAT CTAGTATAGT CCGGAAAGAA GCAAAATCAG ACAGTGCAAG CAGTGACAAC 
TCAGCAGGTC TACTGTTCAC ACCATCCTTT AGTGAGAATT GTGAAAGTGA AGTGTATCAG 
TGCTGGAGTC TACATAAAAG ACACAAGAGT CCTGTGGAGT CAGATCTGAA AAAACAGATG 
GAATGGGAAA AGAGGAACAG GCATCTAAGC AGTGGCAATG TTGAGGAATT ATTTATCAGT 
AAGTCTGATG GACAGAATAA CACTGAAAAC ATGGTGGACA AACTTGGAGC AGATGCAAAA 
ATACTTAGCA CTGTATATAA GCAACACATG GCACGGCCCA AGACCCTGGA CTTAAATAAA 
ATGAATCCCA GAAAGTCAAA GGAGGTTGTT GAAAAAGAAT CTAGTGATGA TGAGTCTCCC 
ATAGGAGGAG CAGATGACAA AGTGGACTCC CCTGTTATTT TTGACCTTGA AGATCTAGAT 
AATGATTCTG ATGAACTTAA ATCAGCAAAA CCATGTGTCC GGAAGGCCAA ACTTGCTCAA 
AGAAGTATCA GCATGCCCTT TTCATCTTCC ATGTTTCTGG CTAAGCCAGC TGAAAAAACA 
GACGTGAAGG CTGGATTAGA CCCTTTGTCA TTGCTGGCAG CTGAAACAGA ACAGGCAGAA 
GTAGAACAGG AGGAGGAAGA TGACAAAAGC TTACTAACAC CATCTGCTAA AAGGGACCTT 
GCAGAGGAAA TAGAAATGTA CATGAACAAT ATAAGCAGCC CCCTTGCAAG TCGAACCCCT 
AGCATGGACC TTGACAGATC CAGTTCTGAA CTAAATAAAA CATGTAAAGC TGTAGCACTC 
AACAGGCGCT TGAGTGTTCC TGCTAGATCC CCACAAGAAC CAAGACTAAC CAGATCAATT 
ACTGCCCACA GTAAACGCGA CAAAAGACAA AGAAGCAGGC TCTGGTCTTC TCCTGCTGTT 
TCCGAGAACA ATCCTCAGAA TGAGCAAAGC CCAGAATCTG CCCTTGCTTT AATGTCCCCA 
ACATCATTTA ACATAGATAC ACTTCTCACG CCAACCTTTG ATGTTCTTAG GAGCAACATG 
TTCTCAGCTG GCAAAGGAGT AGCCAAGAAG GCCAGCAAAT GGTATTCAAA GATCGCAAAT 
TATGCGGGGC CTGCAAAGGA TCAGAATTCT GACCGAGCCA GTCTGTCTTC AGGAGGAGCA 
GGGGATACTG AATCCTACTC TTTAAATGAT GATGATTTTG ATGGCAGTGC TTCTCTGCAT 
GAGACTGATG TCTTTCCTGG AACCAATACC ATTGGTGTAA GCAGATGCAG CTTGGAGAGT 
AGCACATCTC AAGACAGTAA CTCTAAACAA CTTTACAATA ATGGTCTGCC ATCAACAGCA 
TCTCCAAACT ACAAGAGTGA GATTCTTTCA TCCTGCAATT CCAGTAGCAC TAGTGTCTTC 
CAGAACTATG CTATGGAGGT TCTGATATCT AGCTGTTCAC GTTGCTTGAC CTGTGACGGT 
CTTGTTCATG ATGAGGAGAT TATGGCAGGC TGGACGGCAG ATGACTCAAA CCTTAACACC 
ACATGTCCTT TTTGCGGGAA CTCATTTCTT CCATCTTTAA ACGTTGAAAT TAGGGACCTG 
CGACGCCCTG GAAGGTACTT TCTGAAGGCC AGCCCATCTG CAGAAAGTAT GCCGCATTCT 
TCAGTATCCT CACAAAGACC TAACTCAGAT ATTCTGACTC CGGCTTCTCC CTGTGCTTCC 
ACCAAAGTCA AGGATTTTAG TTTGTGCAGT AAAACCAAAC AAGAGAGAAC TCGGTCTGTA 
ACTGTTGAGA TCCCAGTGGG GAAGAGAACC AGAGATCTCA ATGGGTCCTT ATGCCCGAAT 
CAGCCCATTG CACGAAGTAT CAGCACATTT GGTCCCTTAG ATGAACAGCC ATCTCATGAC 
CAGAGCCATG TTATTGCCAC TGGCAGCCTC CCAACTTGCT TCCAGGCACC TCCTGAGTCA 
GGATTGGAAT GGAAGATGTC AAATCCAGAG CCAATCACAG TCCCTTACCT TAGCCCGCTG 
GTTGTCTGGA AGGAGCTGGA GAGCCTGCTA GAAAATGAGG GTGACCAATC GATTACAGTT 
TCTGGATTTG TGGACCATCA TCCGATAGTA TTCTGGAATT TGGTGTGGTA TTTCAGGAGG 
CTGGATCTCC CAAGTAACCT CCCGGGATTA ATTCTGTCTT CGGAACAATG CAATAAAAAT 
TCAAAGGTCT CCAGGAGCTG TATGTCTGCA GACAGCAAAC ATGTCCTTAT TCAAGTGCTG 
TGGGACAATC TGAAATTGCA CCAGGATCCC CGGCAGCCTC TCTACATCCT CTGGAACACT 
ATTAGTCAAA ACCGCACTGT ATTGTTTGAT ACACAGGAAC ATGCGCTTGC TGTGCCACAG 
ATCAAAGAGG AGGAATTGTT TGATCAGCAG TTTCTCAGAG GGATAGTAAG AAGCATAAAG 
AACAACAATG TTTATGGTCC TGTGAGCCAG ATATTGGACT GTATTAAACA GGTCCCTGGA 
AGAACGAGGC AGAGGAGTCT GTACAGGGAA ATTCTTTTTC TTTCTCTTGT TGCCTTGGGA 
AGAGAGAATA TCGATATTGA TGTATTTGAT CGGGAATATA AAACTGCATA CGAGCGCCTC 
ACCCCTGAGC AGCTGAACTG CACACAGATT TGTGATCGGC CTCCCAGCAT TGGAGTGATG 
GAGTGCAGGA AGACTTTCGG ACAGCCATTC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755113.1
CDS	257..6052
Translation	MMEDKGPRVADYFVVTGLTDSSKPLEEEIHFNDACHKTAKPKEPITDVTVIIKSLGEEVPQGYVCIDLTPTGLSADLNNGSIRGPQIYLCYRRGRDKPPLTDLGVLYEWKERLKQGCKIIQATPYGRPANISGSASSQRVFITYRRAAESMTQNSLAVTDICIIIPSKGETPPHTFCKVEKNLNSSVWGSAVYLCYKKSVAKTNAVSYKAGLICRYPEEDYESFPLPESVPLFCLPMGATIECWPSDSNYPLPVFSTFVLTGASAEKVYGAAIQFYEPYAQDQLTEKQQVHLGLLSSAEGQMDSSKSIHVNKCICLLSHWPFFDAFKKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDTLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQIEGKRNVTWKILPKKACKNLLNTLCNLYQQLAELQHRPREEAFMELAMNDYDFNYGKRMHQLDMEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFELQGFLKSRDRSHHKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVEKLFSERNNADKGGKADVDRPGETRLIELDESHRSEHTVFITPPEIPHLPNGEEPPKNYSYNGFPVLILDLFDTPDGLMKSHKLSTKVTTPCSPSPMFRRTKQEIRSAHKIAKQYSSIPQMWSRCLLRHCYGLWFICLPAYVKFCHSKVRALRTAYDVLRKMHMKKIDSPDEVCYRVLMQLCGQYGQPVLAVKVLFEMRKVGVQPNAITYGYYNKAVLESVWPSGNRGGYFLWIKVRNVVLAVAQFKQALIKQHASAHQTPASDCSDLDAVSHGSLDSSVDMNPTEQPSFSTDVTCADPADDRSSTGGQSDLGYSSLSKEEVRRGAVHDIQEEKEDSRDSDSSSSSDSESSKSSRDCVPQGTGSQGQQTLASTSSIVRKEAKSDSASSDNSAGLLFTPSFSENCESEVYQCWSLHKRHKSPVESDLKKQMEWEKRNRHLSSGNVEELFISKSDGQNNTENMVDKLGADAKILSTVYKQHMARPKTLDLNKMNPRKSKEVVEKESSDDESPIGGADDKVDSPVIFDLEDLDNDSDELKSAKPCVRKAKLAQRSISMPFSSSMFLAKPAEKTDVKAGLDPLSLLAAETEQAEVEQEEEDDKSLLTPSAKRDLAEEIEMYMNNISSPLASRTPSMDLDRSSSELNKTCKAVALNRRLSVPARSPQEPRLTRSITAHSKRDKRQRSRLWSSPAVSENNPQNEQSPESALALMSPTSFNIDTLLTPTFDVLRSNMFSAGKGVAKKASKWYSKIANYAGPAKDQNSDRASLSSGGAGDTESYSLNDDDFDGSASLHETDVFPGTNTIGVSRCSLESSTSQDSNSKQLYNNGLPSTASPNYKSEILSSCNSSSTSVFQNYAMEVLISSCSRCLTCDGLVHDEEIMAGWTADDSNLNTTCPFCGNSFLPSLNVEIRDLRRPGRYFLKASPSAESMPHSSVSSQRPNSDILTPASPCASTKVKDFSLCSKTKQERTRSVTVEIPVGKRTRDLNGSLCPNQPIARSISTFGPLDEQPSHDQSHVIATGSLPTCFQAPPESGLEWKMSNPEPITVPYLSPLVVWKELESLLENEGDQSITVSGFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEQCNKNSKVSRSCMSADSKHVLIQVLWDNLKLHQDPRQPLYILWNTISQNRTVLFDTQEHALAVPQIKEEELFDQQFLRGIVRSIKNNNVYGPVSQILDCIKQVPGRTRQRSLYREILFLSLVALGRENIDIDVFDREYKTAYERLTPEQLNCTQICDRPPSIGVMECRKTFGQPFL

Target ORF information:

RefSeq Version	XM_031899253.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899253.1

ORF Insert Sequence:

ATGATGGAAG ACAAAGGGCC CCGTGTGGCT GACTACTTTG TAGTTACAGG ACTGACAGAT 
TCATCAAAAC CACTTGAGGA GGAGATCCAC TTTAATGATG CCTGTCATAA AACAGCTAAA 
CCAAAGGAAC CCATTACAGA TGTGACTGTG ATAATCAAAT CCTTAGGGGA GGAAGTGCCA 
CAGGGATATG TATGCATAGA CCTTACCCCA ACTGGACTAT CAGCCGATCT CAATAATGGA 
AGTATTCGAG GGCCACAGAT ATATCTTTGT TACAGGAGAG GAAGAGACAA ACCACCTCTA 
ACAGATCTTG GGGTATTGTA CGAATGGAAG GAAAGATTAA AACAGGGCTG CAAGATTATT 
CAGGCTACTC CATATGGCCG TCCAGCTAAT ATAAGCGGTA GTGCATCCTC ACAGAGAGTA 
TTTATCACAT ACCGCAGAGC TGCTGAGAGC ATGACCCAGA ATTCCTTGGC TGTAACTGAT 
ATCTGCATCA TTATACCAAG CAAAGGAGAG ACTCCACCAC ACACATTTTG CAAAGTGGAA 
AAGAATCTGA ACAGCAGCGT GTGGGGCTCT GCTGTGTACC TGTGCTACAA GAAATCAGTT 
GCCAAAACAA ATGCAGTATC CTATAAAGCT GGGTTGATAT GCAGGTATCC AGAGGAGGAT 
TATGAATCAT TTCCTCTGCC GGAATCAGTC CCACTATTTT GTCTACCCAT GGGGGCAACA 
ATTGAATGTT GGCCATCTGA CAGTAATTAT CCACTCCCTG TTTTTTCCAC TTTTGTTTTA 
ACTGGAGCAT CAGCTGAAAA GGTGTATGGA GCTGCTATTC AGTTTTATGA GCCATATGCT 
CAAGATCAGC TGACTGAAAA GCAACAAGTA CATTTAGGCC TCTTGAGCAG TGCAGAGGGT 
CAGATGGACT CTTCTAAAAG TATCCATGTC AACAAGTGCA TCTGCCTGCT GTCCCACTGG 
CCTTTTTTTG ATGCTTTCAA GAAATTTCTC ACTTTCCTTT ACCGCTATTC AATATCGGGT 
CCTCATGTGC TGCCAATAGA AAAACATATC TCCCACTTCA TGCACAAAGT CCCATTCCCA 
TCTCCACAGA GACCACGCAT TCTAGTTCAG CTTTCCCCGC ATGATACTCT GATCCTAAGC 
CAGCCTGTGT CCTCACCACT TCCACTGAGT GGTGGAAAGT TTTCAACGTT GCTGCAGAAT 
TTGGGACCTG AAAATGCTGT GACTCTGCTT GTATTTGCTG TCACGGAGCA TAAGATTCTG 
ATCCATTCGT TGCGCCCCTC GGTTCTCACA AGCGTCACAG AAGCTTTAGT GTCTATGATC 
TTCCCTTTCC ATTGGCCGTG TCCTTACATT CCACTTTGCC CTTTGGCCCT GGCAGACGTA 
CTAAGCGCCC CATGCCCCTT CATCGTGGGA ATTGATTCTC GCTACTTTGA TCTGTACGAC 
CCCCCTCCGG ATGTAAGCTG TGTTGACCTA GATACCAACA CCATCTCTCA AATTGAAGGC 
AAAAGAAATG TAACATGGAA AATCCTACCG AAGAAAGCTT GTAAGAACCT GCTCAACACT 
CTTTGTAACT TGTACCAGCA ACTGGCAGAG TTGCAACACA GACCACGGGA AGAAGCTTTT 
ATGGAACTTG CAATGAATGA CTATGACTTT AACTATGGAA AAAGAATGCA TCAGCTGGAC 
ATGGAGATTC AGGAAGCATT CCTGTGCTTT ATGGCCTCTA TTTTAAAAGG ATACAGATCG 
TATCTTCGGC CAATTACCCA AGCACCATCA GAGACTGCAA CAGATGCCAG CTCTCTCTTC 
GAACTGCAAG GTTTCTTAAA AAGTCGTGAT CGCTCACACC ACAAGTTTTA CAACATGATG 
ACCAAGACTC AGATGTTCAT CCGCTTTATT GAGGAGTGTT CCTTCGTCAG CGATAAGGAT 
GCTAGCCTGG CTTTCTTTGA TGACTGTGTT GAGAAGCTTT TCTCAGAGAG GAATAATGCA 
GATAAAGGTG GCAAGGCGGA TGTGGATCGA CCAGGAGAGA CACGTCTTAT TGAACTGGAT 
GAGTCTCACA GAAGTGAGCA TACAGTGTTC ATCACACCAC CAGAGATTCC CCACTTACCA 
AATGGCGAGG AGCCCCCTAA GAACTATAGC TACAATGGGT TCCCTGTCCT TATCTTGGAT 
CTCTTTGACA CGCCAGATGG ACTCATGAAA AGTCACAAAC TTAGTACAAA AGTCACCACA 
CCCTGCAGTC CTTCTCCAAT GTTTAGAAGA ACAAAACAGG AAATCCGGTC AGCACATAAA 
ATAGCAAAGC AGTACTCCTC CATCCCCCAG ATGTGGTCCA GGTGTCTACT GAGACATTGC 
TATGGACTTT GGTTTATCTG TCTGCCCGCA TATGTGAAAT TCTGCCATTC CAAAGTGAGA 
GCACTGCGCA CAGCCTACGA TGTGTTGCGA AAAATGCACA TGAAAAAGAT TGACTCTCCT 
GATGAGGTTT GCTATAGGGT TCTAATGCAG TTGTGTGGCC AATATGGGCA GCCTGTCCTT 
GCTGTTAAAG TTCTCTTTGA AATGAGAAAG GTCGGCGTGC AGCCAAATGC TATAACATAT 
GGCTATTATA ATAAGGCTGT CCTGGAAAGC GTATGGCCCT CTGGGAATAG AGGGGGTTAC 
TTTCTGTGGA TAAAGGTGAG GAATGTGGTG CTAGCAGTAG CCCAGTTCAA ACAGGCTTTG 
ATAAAGCAAC ATGCTTCAGC TCATCAAACT CCTGCTTCAG ATTGCAGTGA TTTGGACGCA 
GTCAGCCATG GCAGTTTGGA TAGTTCTGTT GACATGAACC CTACAGAACA GCCTTCATTC 
AGTACAGATG TAACATGTGC TGATCCAGCT GACGACCGAT CTAGTACAGG AGGCCAGTCA 
GACCTTGGCT ACAGTTCATT ATCAAAAGAA GAAGTAAGAA GAGGAGCCGT GCATGACATA 
CAGGAGGAGA AGGAAGACTC CAGAGATAGT GACTCCAGTT CGTCATCTGA TTCAGAAAGT 
TCCAAGAGCA GCAGAGACTG TGTGCCACAA GGAACTGGCA GTCAGGGTCA ACAAACATTG 
GCAAGCACAT CTAGTATAGT CCGGAAAGAA GCAAAATCAG ACAGTGCAAG CAGTGACAAC 
TCAGCAGGTC TACTGTTCAC ACCATCCTTT AGTGAGAATT GTGAAAGTGA AGTGTATCAG 
TGCTGGAGTC TACATAAAAG ACACAAGAGT CCTGTGGAGT CAGATCTGAA AAAACAGATG 
GAATGGGAAA AGAGGAACAG GCATCTAAGC AGTGGCAATG TTGAGGAATT ATTTATCAGT 
AAGTCTGATG GACAGAATAA CACTGAAAAC ATGGTGGACA AACTTGGAGC AGATGCAAAA 
ATACTTAGCA CTGTATATAA GCAACACATG GCACGGCCCA AGACCCTGGA CTTAAATAAA 
ATGAATCCCA GAAAGTCAAA GGAGGTTGTT GAAAAAGAAT CTAGTGATGA TGAGTCTCCC 
ATAGGAGGAG CAGATGACAA AGTGGACTCC CCTGTTATTT TTGACCTTGA AGATCTAGAT 
AATGATTCTG ATGAACTTAA ATCAGCAAAA CCATGTGTCC GGAAGGCCAA ACTTGCTCAA 
AGAAGTATCA GCATGCCCTT TTCATCTTCC ATGTTTCTGG CTAAGCCAGC TGAAAAAACA 
GACGTGAAGG CTGGATTAGA CCCTTTGTCA TTGCTGGCAG CTGAAACAGA ACAGGCAGAA 
GTAGAACAGG AGGAGGAAGA TGACAAAAGC TTACTAACAC CATCTGCTAA AAGGGACCTT 
GCAGAGGAAA TAGAAATGTA CATGAACAAT ATAAGCAGCC CCCTTGCAAG TCGAACCCCT 
AGCATGGACC TTGACAGATC CAGTTCTGAA CTAAATAAAA CATGTAAAGC TGTAGCACTC 
AACAGGCGCT TGAGTGTTCC TGCTAGATCC CCACAAGAAC CAAGACTAAC CAGATCAATT 
ACTGCCCACA GTAAACGCGA CAAAAGACAA AGAAGCAGGC TCTGGTCTTC TCCTGCTGTT 
TCCGAGAACA ATCCTCAGAA TGAGCAAAGC CCAGAATCTG CCCTTGCTTT AATGTCCCCA 
ACATCATTTA ACATAGATAC ACTTCTCACG CCAACCTTTG ATGTTCTTAG GAGCAACATG 
TTCTCAGCTG GCAAAGGAGT AGCCAAGAAG GCCAGCAAAT GGTATTCAAA GATCGCAAAT 
TATGCGGGGC CTGCAAAGGA TCAGAATTCT GACCGAGCCA GTCTGTCTTC AGGAGGAGCA 
GGGGATACTG AATCCTACTC TTTAAATGAT GATGATTTTG ATGGCAGTGC TTCTCTGCAT 
GAGACTGATG TCTTTCCTGG AACCAATACC ATTGGTGTAA GCAGATGCAG CTTGGAGAGT 
AGCACATCTC AAGACAGTAA CTCTAAACAA CTTTACAATA ATGGTCTGCC ATCAACAGCA 
TCTCCAAACT ACAAGAGTGA GATTCTTTCA TCCTGCAATT CCAGTAGCAC TAGTGTCTTC 
CAGAACTATG CTATGGAGGT TCTGATATCT AGCTGTTCAC GTTGCTTGAC CTGTGACGGT 
CTTGTTCATG ATGAGGAGAT TATGGCAGGC TGGACGGCAG ATGACTCAAA CCTTAACACC 
ACATGTCCTT TTTGCGGGAA CTCATTTCTT CCATCTTTAA ACGTTGAAAT TAGGGACCTG 
CGACGCCCTG GAAGGTACTT TCTGAAGGCC AGCCCATCTG CAGAAAGTAT GCCGCATTCT 
TCAGTATCCT CACAAAGACC TAACTCAGAT ATTCTGACTC CGGCTTCTCC CTGTGCTTCC 
ACCAAAGTCA AGGATTTTAG TTTGTGCAGT AAAACCAAAC AAGAGAGAAC TCGGTCTGTA 
ACTGTTGAGA TCCCAGTGGG GAAGAGAACC AGAGATCTCA ATGGGTCCTT ATGCCCGAAT 
CAGCCCATTG CACGAAGTAT CAGCACATTT GGTCCCTTAG ATGAACAGCC ATCTCATGAC 
CAGAGCCATG TTATTGCCAC TGGCAGCCTC CCAACTTGCT TCCAGGCACC TCCTGAGTCA 
GGATTGGAAT GGAAGATGTC AAATCCAGAG CCAATCACAG TCCCTTACCT TAGCCCGCTG 
GTTGTCTGGA AGGAGCTGGA GAGCCTGCTA GAAAATGAGG GTGACCAATC GATTACAGTT 
TCTGGATTTG TGGACCATCA TCCGATAGTA TTCTGGAATT TGGTGTGGTA TTTCAGGAGG 
CTGGATCTCC CAAGTAACCT CCCGGGATTA ATTCTGTCTT CGGAACAATG CAATAAAAAT 
TCAAAGGTCT CCAGGAGCTG TATGTCTGCA GACAGCAAAC ATGTCCTTAT TCAAGTGCTG 
TGGGACAATC TGAAATTGCA CCAGGATCCC CGGCAGCCTC TCTACATCCT CTGGAACACT 
ATTAGTCAAA ACCGCACTGT ATTGTTTGAT ACACAGGAAC ATGCGCTTGC TGTGCCACAG 
ATCAAAGAGG AGGAATTGTT TGATCAGCAG TTTCTCAGAG GGATAGTAAG AAGCATAAAG 
AACAACAATG TTTATGGTCC TGTGAGCCAG ATATTGGACT GTATTAAACA GGTCCCTGGA 
AGAACGAGGC AGAGGAGTCT GTACAGGGAA ATTCTTTTTC TTTCTCTTGT TGCCTTGGGA 
AGAGAGAATA TCGATATTGA TGTATTTGAT CGGGAATATA AAACTGCATA CGAGCGCCTC 
ACCCCTGAGC AGCTGAACTG CACACAGATT TGTGATCGGC CTCCCAGCAT TGGAGTGATG 
GAGTGCAGGA AGACTTTCGG ACAGCCATTC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4a ( XM_031899255.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031755115.1 Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X6, mRNA.

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CloneID	OXa55249
Clone ID Related Accession (Same CDS sequence)	XM_031899255.1
Accession Version	XM_031899255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	C-myc promoter-binding protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACTT TTGAGGAAGA GCAAGTAAGC AGTTGTCCTA CTCCAAAACG GAAGATGATG 
GAAGACAAAG GGCCCCGTGT GGCTGACTAC TTTGTAGTTA CAGGACTGAC AGATTCATCA 
AAACCACTTG AGGAGGAGAT CCACTTTAAT GATGCCTGTC ATAAAACAGC TAAACCAAAG 
GAACCCATTA CAGATGTGAC TGTGATAATC AAATCCTTAG GGGAGGAAGT GCCACAGGGA 
TATGTATGCA TAGACCTTAC CCCAACTGGA CTATCAGCCG ATCTCAATAA TGGAAGTATT 
CGAGGGCCAC AGATATATCT TTGTTACAGG AGAGGAAGAG ACAAACCACC TCTAACAGAT 
CTTGGGGTAT TGTACGAATG GAAGGAAAGA TTAAAACAGG GCTGCAAGAT TATTCAGGCT 
ACTCCATATG GCCGTCCAGC TAATATAAGC GGTAGTGCAT CCTCACAGAG AGTATTTATC 
ACATACCGCA GAGCTGCTGA GAGCATGACC CAGAATTCCT TGGCTGTAAC TGATATCTGC 
ATCATTATAC CAAGCAAAGG AGAGACTCCA CCACACACAT TTTGCAAAGT GGAAAAGAAT 
CTGAACAGCA GCGTGTGGGG CTCTGCTGTG TACCTGTGCT ACAAGAAATC AGTTGCCAAA 
ACAAATGCAG TATCCTATAA AGCTGGGTTG ATATGCAGGT ATCCAGAGGA GGATTATGAA 
TCATTTCCTC TGCCGGAATC AGTCCCACTA TTTTGTCTAC CCATGGGGGC AACAATTGAA 
TGTTGGCCAT CTGACAGTAA TTATCCACTC CCTGTTTTTT CCACTTTTGT TTTAACTGGA 
GCATCAGCTG AAAAGGTGTA TGGAGCTGCT ATTCAGTTTT ATGAGCCATA TGCTCAAGAT 
CAGCTGACTG AAAAGCAACA AGTACATTTA GGCCTCTTGA GCAGTGCAGA GGGTCAGATG 
GACTCTTCTA AAAGTATCCA TGTCAACAAG TGCATCTGCC TGCTGTCCCA CTGGCCTTTT 
TTTGATGCTT TCAAGAAATT TCTCACTTTC CTTTACCGCT ATTCAATATC GGGTCCTCAT 
GTGCTGCCAA TAGAAAAACA TATCTCCCAC TTCATGCACA AAGTCCCATT CCCATCTCCA 
CAGAGACCAC GCATTCTAGT TCAGCTTTCC CCGCATGATA CTCTGATCCT AAGCCAGCCT 
GTGTCCTCAC CACTTCCACT GAGTGGTGGA AAGTTTTCAA CGTTGCTGCA GAATTTGGGA 
CCTGAAAATG CTGTGACTCT GCTTGTATTT GCTGTCACGG AGCATAAGAT TCTGATCCAT 
TCGTTGCGCC CCTCGGTTCT CACAAGCGTC ACAGAAGCTT TAGTGTCTAT GATCTTCCCT 
TTCCATTGGC CGTGTCCTTA CATTCCACTT TGCCCTTTGG CCCTGGCAGA CGTACTAAGC 
GCCCCATGCC CCTTCATCGT GGGAATTGAT TCTCGCTACT TTGATCTGTA CGACCCCCCT 
CCGGATGTAA GCTGTGTTGA CCTAGATACC AACACCATCT CTCAAATTGA AGGCAAAAGA 
AATGTAACAT GGAAAATCCT ACCGAAGAAA GCTTGTAAGA ACCTGCTCAA CACTCTTTGT 
AACTTGTACC AGCAACTGGC AGAGTTGCAA CACAGACCAC GGGAAGAAGC TTTTATGGAA 
CTTGCAATGA ATGACTATGA CTTTAACTAT GGAAAAAGAA TGCATCAGCT GGACATGGAG 
ATTCAGGAAG CATTCCTGTG CTTTATGGCC TCTATTTTAA AAGGATACAG ATCGTATCTT 
CGGCCAATTA CCCAAGCACC ATCAGAGACT GCAACAGATG CCAGCTCTCT CTTCGAACTG 
CAAGGTTTCT TAAAAAGTCG TGATCGCTCA CACCACAAGT TTTACAACAT GATGACCAAG 
ACTCAGATGT TCATCCGCTT TATTGAGGAG TGTTCCTTCG TCAGCGATAA GGATGCTAGC 
CTGGCTTTCT TTGATGACTG TGTTGAGAAG CTTTTCTCAG AGAGGAATAA TGCAGATAAA 
GGTGGCAAGG CGGATGTGGA TCGACCAGGA GAGACACGTC TTATTGAACT GGATGAGTCT 
CACAGAAGTG AGCATACAGT GTTCATCACA CCACCAGAGA TTCCCCACTT ACCAAATGGC 
GAGGAGCCCC CTAAGAACTA TAGCTACAAT GGGTTCCCTG TCCTTATCTT GGATCTCTTT 
GACACGCCAG ATGGACTCAT GAAAAGTCAC AAACTTAGTA CAAAAGTCAC CACACCCTGC 
AGTCCTTCTC CAATGTTTAG AAGAACAAAA CAGGAAATCC GGTCAGCACA TAAAATAGCA 
AAGCAGTACT CCTCCATCCC CCAGATGTGG TCCAGGTGTC TACTGAGACA TTGCTATGGA 
CTTTGGTTTA TCTGTCTGCC CGCATATGTG AAATTCTGCC ATTCCAAAGT GAGAGCACTG 
CGCACAGCCT ACGATGTGTT GCGAAAAATG CACATGAAAA AGATTGACTC TCCTGATGAG 
GTTTGCTATA GGGTTCTAAT GCAGTTGTGT GGCCAATATG GGCAGCCTGT CCTTGCTGTT 
AAAGTTCTCT TTGAAATGAG AAAGGTCGGC GTGCAGCCAA ATGCTATAAC ATATGGCTAT 
TATAATAAGG CTGTCCTGGA AAGCGTATGG CCCTCTGGGA ATAGAGGGGG TTACTTTCTG 
TGGATAAAGG TGAGGAATGT GGTGCTAGCA GTAGCCCAGT TCAAACAGGC TTTGATAAAG 
CAACATGCTT CAGCTCATCA AACTCCTGCT TCAGATTGCA GTGATTTGGA CGCAGTCAGC 
CATGGCAGTT TGGATAGTTC TGTTGACATG AACCCTACAG AACAGCCTTC ATTCAGTACA 
GATGTAACAT GTGCTGATCC AGCTGACGAC CGATCTAGTA CAGGAGGCCA GTCAGACCTT 
GGCTACAGTT CATTATCAAA AGAAGAAGTA AGAAGAGGAG CCGTGCATGA CATACAGGAG 
GAGAAGGAAG ACTCCAGAGA TAGTGACTCC AGTTCGTCAT CTGATTCAGA AAGTTCCAAG 
AGCAGCAGAG ACTGTGTGCC ACAAGGAACT GGCAGTCAGG GTCAACAAAC ATTGGCAAGC 
ACATCTAGTA TAGTCCGGAA AGAAGCAAAA TCAGACAGTG CAAGCAGTGA CAACTCAGCA 
GGTCTACTGT TCACACCATC CTTTAGTGAG AATTGTGAAA GTGAAGTGTA TCAGTGCTGG 
AGTCTACATA AAAGACACAA GAGTCCTGTG GAGTCAGATC TGAAAAAACA GATGGAATGG 
GAAAAGAGGA ACAGGCATCT AAGCAGTGGC AATGTTGAGG AATTATTTAT CAGTAAGTCT 
GATGGACAGA ATAACACTGA AAACATGGTG GACAAACTTG GAGCAGATGC AAAAATACTT 
AGCACTGTAT ATAAGCAACA CATGGCACGG CCCAAGACCC TGGACTTAAA TAAAATGAAT 
CCCAGAAAGT CAAAGGAGGT TGTTGAAAAA GAATCTAGTG ATGATGAGTC TCCCATAGGA 
GGAGCAGATG ACAAAGTGGA CTCCCCTGTT ATTTTTGACC TTGAAGATCT AGATAATGAT 
TCTGATGAAC TTAAATCAGC AAAACCATGT GTCCGGAAGG CCAAACTTGC TCAAAGAAGT 
ATCAGCATGC CCTTTTCATC TTCCATGTTT CTGGCTAAGC CAGCTGAAAA AACAGACGTG 
AAGGCTGGAT TAGACCCTTT GTCATTGCTG GCAGCTGAAA CAGAACAGGC AGAAGTAGAA 
CAGGAGGAGG AAGATGACAA AAGCTTACTA ACACCATCTG CTAAAAGGGA CCTTGCAGAG 
GAAATAGAAA TGTACATGAA CAATATAAGC AGCCCCCTTG CAAGTCGAAC CCCTAGCATG 
GACCTTGACA GATCCAGTTC TGAACTAAAT AAAACATGTA AAGCTGTAGC ACTCAACAGG 
CGCTTGAGTG TTCCTGCTAG ATCCCCACAA GAACCAAGAC TAACCAGATC AATTACTGCC 
CACAGTAAAC GCGACAAAAG ACAAAGAAGC AGGCTCTGGT CTTCTCCTGC TGTTTCCGAG 
AACAATCCTC AGAATGAGCA AAGCCCAGAA TCTGCCCTTG CTTTAATGTC CCCAACATCA 
TTTAACATAG ATACACTTCT CACGCCAACC TTTGATGTTC TTAGGAGCAA CATGTTCTCA 
GCTGGCAAAG GAGTAGCCAA GAAGGCCAGC AAATGGTATT CAAAGATCGC AAATTATGCG 
GGGCCTGCAA AGGATCAGAA TTCTGACCGA GCCAGTCTGT CTTCAGGAGG AGCAGGGGAT 
ACTGAATCCT ACTCTTTAAA TGATGATGAT TTTGATGGCA GTGCTTCTCT GCATGAGACT 
GATGTCTTTC CTGGAACCAA TACCATTGGT GTAAGCAGAT GCAGCTTGGA GAGTAGCACA 
TCTCAAGACA GTAACTCTAA ACAACTTTAC AATAATGGTC TGCCATCAAC AGCATCTCCA 
AACTACAAGA GTGAGATTCT TTCATCCTGC AATTCCAGTA GCACTAGTGT CTTCCAGAAC 
TATGCTATGG AGGTTCTGAT ATCTAGCTGT TCACGTTGCT TGACCTGTGA CGGTCTTGTT 
CATGATGAGG AGATTATGGC AGGCTGGACG GCAGATGACT CAAACCTTAA CACCACATGT 
CCTTTTTGCG GGAACTCATT TCTTCCATCT TTAAACGTTG AAATTAGGGA CCTGCGACGC 
CCTGGAAGTA AAACCAAACA AGAGAGAACT CGGTCTGTAA CTGTTGAGAT CCCAGTGGGG 
AAGAGAACCA GAGATCTCAA TGGGTCCTTA TGCCCGAATC AGCCCATTGC ACGAAGTATC 
AGCACATTTG GTCCCTTAGA TGAACAGCCA TCTCATGACC AGAGCCATGT TATTGCCACT 
GGCAGCCTCC CAACTTGCTT CCAGGCACCT CCTGAGTCAG GATTGGAATG GAAGATGTCA 
AATCCAGAGC CAATCACAGT CCCTTACCTT AGCCCGCTGG TTGTCTGGAA GGAGCTGGAG 
AGCCTGCTAG AAAATGAGGG TGACCAATCG ATTACAGTTT CTGGATTTGT GGACCATCAT 
CCGATAGTAT TCTGGAATTT GGTGTGGTAT TTCAGGAGGC TGGATCTCCC AAGTAACCTC 
CCGGGATTAA TTCTGTCTTC GGAACAATGC AATAAAAATT CAAAGGTCTC CAGGAGCTGT 
ATGTCTGCAG ACAGCAAACA TGTCCTTATT CAAGTGCTGT GGGACAATCT GAAATTGCAC 
CAGGATCCCC GGCAGCCTCT CTACATCCTC TGGAACACTA TTAGTCAAAA CCGCACTGTA 
TTGTTTGATA CACAGGAACA TGCGCTTGCT GTGCCACAGA TCAAAGAGGA GGAATTGTTT 
GATCAGCAGT TTCTCAGAGG GATAGTAAGA AGCATAAAGA ACAACAATGT TTATGGTCCT 
GTGAGCCAGA TATTGGACTG TATTAAACAG GTCCCTGGAA GAACGAGGCA GAGGAGTCTG 
TACAGGGAAA TTCTTTTTCT TTCTCTTGTT GCCTTGGGAA GAGAGAATAT CGATATTGAT 
GTATTTGATC GGGAATATAA AACTGCATAC GAGCGCCTCA CCCCTGAGCA GCTGAACTGC 
ACACAGATTT GTGATCGGCC TCCCAGCATT GGAGTGATGG AGTGCAGGAA GACTTTCGGA 
CAGCCATTCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031755115.1
CDS	48..5762
Translation	MGTFEEEQVSSCPTPKRKMMEDKGPRVADYFVVTGLTDSSKPLEEEIHFNDACHKTAKPKEPITDVTVIIKSLGEEVPQGYVCIDLTPTGLSADLNNGSIRGPQIYLCYRRGRDKPPLTDLGVLYEWKERLKQGCKIIQATPYGRPANISGSASSQRVFITYRRAAESMTQNSLAVTDICIIIPSKGETPPHTFCKVEKNLNSSVWGSAVYLCYKKSVAKTNAVSYKAGLICRYPEEDYESFPLPESVPLFCLPMGATIECWPSDSNYPLPVFSTFVLTGASAEKVYGAAIQFYEPYAQDQLTEKQQVHLGLLSSAEGQMDSSKSIHVNKCICLLSHWPFFDAFKKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDTLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQIEGKRNVTWKILPKKACKNLLNTLCNLYQQLAELQHRPREEAFMELAMNDYDFNYGKRMHQLDMEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFELQGFLKSRDRSHHKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVEKLFSERNNADKGGKADVDRPGETRLIELDESHRSEHTVFITPPEIPHLPNGEEPPKNYSYNGFPVLILDLFDTPDGLMKSHKLSTKVTTPCSPSPMFRRTKQEIRSAHKIAKQYSSIPQMWSRCLLRHCYGLWFICLPAYVKFCHSKVRALRTAYDVLRKMHMKKIDSPDEVCYRVLMQLCGQYGQPVLAVKVLFEMRKVGVQPNAITYGYYNKAVLESVWPSGNRGGYFLWIKVRNVVLAVAQFKQALIKQHASAHQTPASDCSDLDAVSHGSLDSSVDMNPTEQPSFSTDVTCADPADDRSSTGGQSDLGYSSLSKEEVRRGAVHDIQEEKEDSRDSDSSSSSDSESSKSSRDCVPQGTGSQGQQTLASTSSIVRKEAKSDSASSDNSAGLLFTPSFSENCESEVYQCWSLHKRHKSPVESDLKKQMEWEKRNRHLSSGNVEELFISKSDGQNNTENMVDKLGADAKILSTVYKQHMARPKTLDLNKMNPRKSKEVVEKESSDDESPIGGADDKVDSPVIFDLEDLDNDSDELKSAKPCVRKAKLAQRSISMPFSSSMFLAKPAEKTDVKAGLDPLSLLAAETEQAEVEQEEEDDKSLLTPSAKRDLAEEIEMYMNNISSPLASRTPSMDLDRSSSELNKTCKAVALNRRLSVPARSPQEPRLTRSITAHSKRDKRQRSRLWSSPAVSENNPQNEQSPESALALMSPTSFNIDTLLTPTFDVLRSNMFSAGKGVAKKASKWYSKIANYAGPAKDQNSDRASLSSGGAGDTESYSLNDDDFDGSASLHETDVFPGTNTIGVSRCSLESSTSQDSNSKQLYNNGLPSTASPNYKSEILSSCNSSSTSVFQNYAMEVLISSCSRCLTCDGLVHDEEIMAGWTADDSNLNTTCPFCGNSFLPSLNVEIRDLRRPGSKTKQERTRSVTVEIPVGKRTRDLNGSLCPNQPIARSISTFGPLDEQPSHDQSHVIATGSLPTCFQAPPESGLEWKMSNPEPITVPYLSPLVVWKELESLLENEGDQSITVSGFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEQCNKNSKVSRSCMSADSKHVLIQVLWDNLKLHQDPRQPLYILWNTISQNRTVLFDTQEHALAVPQIKEEELFDQQFLRGIVRSIKNNNVYGPVSQILDCIKQVPGRTRQRSLYREILFLSLVALGRENIDIDVFDREYKTAYERLTPEQLNCTQICDRPPSIGVMECRKTFGQPFL

Target ORF information:

RefSeq Version	XM_031899255.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031899255.1

ORF Insert Sequence:

ATGGGAACTT TTGAGGAAGA GCAAGTAAGC AGTTGTCCTA CTCCAAAACG GAAGATGATG 
GAAGACAAAG GGCCCCGTGT GGCTGACTAC TTTGTAGTTA CAGGACTGAC AGATTCATCA 
AAACCACTTG AGGAGGAGAT CCACTTTAAT GATGCCTGTC ATAAAACAGC TAAACCAAAG 
GAACCCATTA CAGATGTGAC TGTGATAATC AAATCCTTAG GGGAGGAAGT GCCACAGGGA 
TATGTATGCA TAGACCTTAC CCCAACTGGA CTATCAGCCG ATCTCAATAA TGGAAGTATT 
CGAGGGCCAC AGATATATCT TTGTTACAGG AGAGGAAGAG ACAAACCACC TCTAACAGAT 
CTTGGGGTAT TGTACGAATG GAAGGAAAGA TTAAAACAGG GCTGCAAGAT TATTCAGGCT 
ACTCCATATG GCCGTCCAGC TAATATAAGC GGTAGTGCAT CCTCACAGAG AGTATTTATC 
ACATACCGCA GAGCTGCTGA GAGCATGACC CAGAATTCCT TGGCTGTAAC TGATATCTGC 
ATCATTATAC CAAGCAAAGG AGAGACTCCA CCACACACAT TTTGCAAAGT GGAAAAGAAT 
CTGAACAGCA GCGTGTGGGG CTCTGCTGTG TACCTGTGCT ACAAGAAATC AGTTGCCAAA 
ACAAATGCAG TATCCTATAA AGCTGGGTTG ATATGCAGGT ATCCAGAGGA GGATTATGAA 
TCATTTCCTC TGCCGGAATC AGTCCCACTA TTTTGTCTAC CCATGGGGGC AACAATTGAA 
TGTTGGCCAT CTGACAGTAA TTATCCACTC CCTGTTTTTT CCACTTTTGT TTTAACTGGA 
GCATCAGCTG AAAAGGTGTA TGGAGCTGCT ATTCAGTTTT ATGAGCCATA TGCTCAAGAT 
CAGCTGACTG AAAAGCAACA AGTACATTTA GGCCTCTTGA GCAGTGCAGA GGGTCAGATG 
GACTCTTCTA AAAGTATCCA TGTCAACAAG TGCATCTGCC TGCTGTCCCA CTGGCCTTTT 
TTTGATGCTT TCAAGAAATT TCTCACTTTC CTTTACCGCT ATTCAATATC GGGTCCTCAT 
GTGCTGCCAA TAGAAAAACA TATCTCCCAC TTCATGCACA AAGTCCCATT CCCATCTCCA 
CAGAGACCAC GCATTCTAGT TCAGCTTTCC CCGCATGATA CTCTGATCCT AAGCCAGCCT 
GTGTCCTCAC CACTTCCACT GAGTGGTGGA AAGTTTTCAA CGTTGCTGCA GAATTTGGGA 
CCTGAAAATG CTGTGACTCT GCTTGTATTT GCTGTCACGG AGCATAAGAT TCTGATCCAT 
TCGTTGCGCC CCTCGGTTCT CACAAGCGTC ACAGAAGCTT TAGTGTCTAT GATCTTCCCT 
TTCCATTGGC CGTGTCCTTA CATTCCACTT TGCCCTTTGG CCCTGGCAGA CGTACTAAGC 
GCCCCATGCC CCTTCATCGT GGGAATTGAT TCTCGCTACT TTGATCTGTA CGACCCCCCT 
CCGGATGTAA GCTGTGTTGA CCTAGATACC AACACCATCT CTCAAATTGA AGGCAAAAGA 
AATGTAACAT GGAAAATCCT ACCGAAGAAA GCTTGTAAGA ACCTGCTCAA CACTCTTTGT 
AACTTGTACC AGCAACTGGC AGAGTTGCAA CACAGACCAC GGGAAGAAGC TTTTATGGAA 
CTTGCAATGA ATGACTATGA CTTTAACTAT GGAAAAAGAA TGCATCAGCT GGACATGGAG 
ATTCAGGAAG CATTCCTGTG CTTTATGGCC TCTATTTTAA AAGGATACAG ATCGTATCTT 
CGGCCAATTA CCCAAGCACC ATCAGAGACT GCAACAGATG CCAGCTCTCT CTTCGAACTG 
CAAGGTTTCT TAAAAAGTCG TGATCGCTCA CACCACAAGT TTTACAACAT GATGACCAAG 
ACTCAGATGT TCATCCGCTT TATTGAGGAG TGTTCCTTCG TCAGCGATAA GGATGCTAGC 
CTGGCTTTCT TTGATGACTG TGTTGAGAAG CTTTTCTCAG AGAGGAATAA TGCAGATAAA 
GGTGGCAAGG CGGATGTGGA TCGACCAGGA GAGACACGTC TTATTGAACT GGATGAGTCT 
CACAGAAGTG AGCATACAGT GTTCATCACA CCACCAGAGA TTCCCCACTT ACCAAATGGC 
GAGGAGCCCC CTAAGAACTA TAGCTACAAT GGGTTCCCTG TCCTTATCTT GGATCTCTTT 
GACACGCCAG ATGGACTCAT GAAAAGTCAC AAACTTAGTA CAAAAGTCAC CACACCCTGC 
AGTCCTTCTC CAATGTTTAG AAGAACAAAA CAGGAAATCC GGTCAGCACA TAAAATAGCA 
AAGCAGTACT CCTCCATCCC CCAGATGTGG TCCAGGTGTC TACTGAGACA TTGCTATGGA 
CTTTGGTTTA TCTGTCTGCC CGCATATGTG AAATTCTGCC ATTCCAAAGT GAGAGCACTG 
CGCACAGCCT ACGATGTGTT GCGAAAAATG CACATGAAAA AGATTGACTC TCCTGATGAG 
GTTTGCTATA GGGTTCTAAT GCAGTTGTGT GGCCAATATG GGCAGCCTGT CCTTGCTGTT 
AAAGTTCTCT TTGAAATGAG AAAGGTCGGC GTGCAGCCAA ATGCTATAAC ATATGGCTAT 
TATAATAAGG CTGTCCTGGA AAGCGTATGG CCCTCTGGGA ATAGAGGGGG TTACTTTCTG 
TGGATAAAGG TGAGGAATGT GGTGCTAGCA GTAGCCCAGT TCAAACAGGC TTTGATAAAG 
CAACATGCTT CAGCTCATCA AACTCCTGCT TCAGATTGCA GTGATTTGGA CGCAGTCAGC 
CATGGCAGTT TGGATAGTTC TGTTGACATG AACCCTACAG AACAGCCTTC ATTCAGTACA 
GATGTAACAT GTGCTGATCC AGCTGACGAC CGATCTAGTA CAGGAGGCCA GTCAGACCTT 
GGCTACAGTT CATTATCAAA AGAAGAAGTA AGAAGAGGAG CCGTGCATGA CATACAGGAG 
GAGAAGGAAG ACTCCAGAGA TAGTGACTCC AGTTCGTCAT CTGATTCAGA AAGTTCCAAG 
AGCAGCAGAG ACTGTGTGCC ACAAGGAACT GGCAGTCAGG GTCAACAAAC ATTGGCAAGC 
ACATCTAGTA TAGTCCGGAA AGAAGCAAAA TCAGACAGTG CAAGCAGTGA CAACTCAGCA 
GGTCTACTGT TCACACCATC CTTTAGTGAG AATTGTGAAA GTGAAGTGTA TCAGTGCTGG 
AGTCTACATA AAAGACACAA GAGTCCTGTG GAGTCAGATC TGAAAAAACA GATGGAATGG 
GAAAAGAGGA ACAGGCATCT AAGCAGTGGC AATGTTGAGG AATTATTTAT CAGTAAGTCT 
GATGGACAGA ATAACACTGA AAACATGGTG GACAAACTTG GAGCAGATGC AAAAATACTT 
AGCACTGTAT ATAAGCAACA CATGGCACGG CCCAAGACCC TGGACTTAAA TAAAATGAAT 
CCCAGAAAGT CAAAGGAGGT TGTTGAAAAA GAATCTAGTG ATGATGAGTC TCCCATAGGA 
GGAGCAGATG ACAAAGTGGA CTCCCCTGTT ATTTTTGACC TTGAAGATCT AGATAATGAT 
TCTGATGAAC TTAAATCAGC AAAACCATGT GTCCGGAAGG CCAAACTTGC TCAAAGAAGT 
ATCAGCATGC CCTTTTCATC TTCCATGTTT CTGGCTAAGC CAGCTGAAAA AACAGACGTG 
AAGGCTGGAT TAGACCCTTT GTCATTGCTG GCAGCTGAAA CAGAACAGGC AGAAGTAGAA 
CAGGAGGAGG AAGATGACAA AAGCTTACTA ACACCATCTG CTAAAAGGGA CCTTGCAGAG 
GAAATAGAAA TGTACATGAA CAATATAAGC AGCCCCCTTG CAAGTCGAAC CCCTAGCATG 
GACCTTGACA GATCCAGTTC TGAACTAAAT AAAACATGTA AAGCTGTAGC ACTCAACAGG 
CGCTTGAGTG TTCCTGCTAG ATCCCCACAA GAACCAAGAC TAACCAGATC AATTACTGCC 
CACAGTAAAC GCGACAAAAG ACAAAGAAGC AGGCTCTGGT CTTCTCCTGC TGTTTCCGAG 
AACAATCCTC AGAATGAGCA AAGCCCAGAA TCTGCCCTTG CTTTAATGTC CCCAACATCA 
TTTAACATAG ATACACTTCT CACGCCAACC TTTGATGTTC TTAGGAGCAA CATGTTCTCA 
GCTGGCAAAG GAGTAGCCAA GAAGGCCAGC AAATGGTATT CAAAGATCGC AAATTATGCG 
GGGCCTGCAA AGGATCAGAA TTCTGACCGA GCCAGTCTGT CTTCAGGAGG AGCAGGGGAT 
ACTGAATCCT ACTCTTTAAA TGATGATGAT TTTGATGGCA GTGCTTCTCT GCATGAGACT 
GATGTCTTTC CTGGAACCAA TACCATTGGT GTAAGCAGAT GCAGCTTGGA GAGTAGCACA 
TCTCAAGACA GTAACTCTAA ACAACTTTAC AATAATGGTC TGCCATCAAC AGCATCTCCA 
AACTACAAGA GTGAGATTCT TTCATCCTGC AATTCCAGTA GCACTAGTGT CTTCCAGAAC 
TATGCTATGG AGGTTCTGAT ATCTAGCTGT TCACGTTGCT TGACCTGTGA CGGTCTTGTT 
CATGATGAGG AGATTATGGC AGGCTGGACG GCAGATGACT CAAACCTTAA CACCACATGT 
CCTTTTTGCG GGAACTCATT TCTTCCATCT TTAAACGTTG AAATTAGGGA CCTGCGACGC 
CCTGGAAGTA AAACCAAACA AGAGAGAACT CGGTCTGTAA CTGTTGAGAT CCCAGTGGGG 
AAGAGAACCA GAGATCTCAA TGGGTCCTTA TGCCCGAATC AGCCCATTGC ACGAAGTATC 
AGCACATTTG GTCCCTTAGA TGAACAGCCA TCTCATGACC AGAGCCATGT TATTGCCACT 
GGCAGCCTCC CAACTTGCTT CCAGGCACCT CCTGAGTCAG GATTGGAATG GAAGATGTCA 
AATCCAGAGC CAATCACAGT CCCTTACCTT AGCCCGCTGG TTGTCTGGAA GGAGCTGGAG 
AGCCTGCTAG AAAATGAGGG TGACCAATCG ATTACAGTTT CTGGATTTGT GGACCATCAT 
CCGATAGTAT TCTGGAATTT GGTGTGGTAT TTCAGGAGGC TGGATCTCCC AAGTAACCTC 
CCGGGATTAA TTCTGTCTTC GGAACAATGC AATAAAAATT CAAAGGTCTC CAGGAGCTGT 
ATGTCTGCAG ACAGCAAACA TGTCCTTATT CAAGTGCTGT GGGACAATCT GAAATTGCAC 
CAGGATCCCC GGCAGCCTCT CTACATCCTC TGGAACACTA TTAGTCAAAA CCGCACTGTA 
TTGTTTGATA CACAGGAACA TGCGCTTGCT GTGCCACAGA TCAAAGAGGA GGAATTGTTT 
GATCAGCAGT TTCTCAGAGG GATAGTAAGA AGCATAAAGA ACAACAATGT TTATGGTCCT 
GTGAGCCAGA TATTGGACTG TATTAAACAG GTCCCTGGAA GAACGAGGCA GAGGAGTCTG 
TACAGGGAAA TTCTTTTTCT TTCTCTTGTT GCCTTGGGAA GAGAGAATAT CGATATTGAT 
GTATTTGATC GGGAATATAA AACTGCATAC GAGCGCCTCA CCCCTGAGCA GCTGAACTGC 
ACACAGATTT GTGATCGGCC TCCCAGCATT GGAGTGATGG AGTGCAGGAA GACTTTCGGA 
CAGCCATTCC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd4a ( XM_018092501.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947990.2 Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X7, mRNA.

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CloneID	OXa55250
Clone ID Related Accession (Same CDS sequence)	XM_018092501.2
Accession Version	XM_018092501.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	C-myc promoter-binding protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018092501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACTT TTGAGGAAGA GCAAGTAAGC AGTTGTCCTA CTCCAAAACG GAAGATGATG 
GAAGACAAAG GGCCCCGTGT GGCTGACTAC TTTGTAGTTA CAGGACTGAC AGATTCATCA 
AAACCACTTG AGGAGGAGAT CCACTTTAAT GATGCCTGTC ATAAAACAGC TAAACCAAAG 
GAACCCATTA CAGATGTGAC TGTGATAATC AAATCCTTAG GGGAGGAAGT GCCACAGGGA 
TATGTATGCA TAGACCTTAC CCCAACTGGA CTATCAGCCG ATCTCAATAA TGGAAGTATT 
CGAGGGCCAC AGATATATCT TTGTTACAGG AGAGGAAGAG ACAAACCACC TCTAACAGAT 
CTTGGGGTAT TGTACGAATG GAAGGAAAGA TTAAAACAGG GCTGCAAGAT TATTCAGGCT 
ACTCCATATG GCCGTCCAGC TAATATAAGC GGTAGTGCAT CCTCACAGAG AGTATTTATC 
ACATACCGCA GAGCTGCTGA GAGCATGACC CAGAATTCCT TGGCTGTAAC TGATATCTGC 
ATCATTATAC CAAGCAAAGG AGAGACTCCA CCACACACAT TTTGCAAAGT GGAAAAGAAT 
CTGAACAGCA GCGTGTGGGG CTCTGCTGTG TACCTGTGCT ACAAGAAATC AGTTGCCAAA 
ACAAATGCAG TATCCTATAA AGCTGGGTTG ATATGCAGGT ATCCAGAGGA GGATTATGAA 
TCATTTCCTC TGCCGGAATC AGTCCCACTA TTTTGTCTAC CCATGGGGGC AACAATTGAA 
TGTTGGCCAT CTGACAGTAA TTATCCACTC CCTGTTTTTT CCACTTTTGT TTTAACTGGA 
GCATCAGCTG AAAAGGTGTA TGGAGCTGCT ATTCAGTTTT ATGAGCCATA TGCTCAAGAT 
CAGCTGACTG AAAAGCAACA AGTACATTTA GGCCTCTTGA GCAGTGCAGA GGGTCAGATG 
GACTCTTCTA AAAGTATCCA TGTCAACAAG TGCATCTGCC TGCTGTCCCA CTGGCCTTTT 
TTTGATGCTT TCAAGAAATT TCTCACTTTC CTTTACCGCT ATTCAATATC GGGTCCTCAT 
GTGCTGCCAA TAGAAAAACA TATCTCCCAC TTCATGCACA AAGTCCCATT CCCATCTCCA 
CAGAGACCAC GCATTCTAGT TCAGCTTTCC CCGCATGATA CTCTGATCCT AAGCCAGCCT 
GTGTCCTCAC CACTTCCACT GAGTGGTGGA AAGTTTTCAA CGTTGCTGCA GAATTTGGGA 
CCTGAAAATG CTGTGACTCT GCTTGTATTT GCTGTCACGG AGCATAAGAT TCTGATCCAT 
TCGTTGCGCC CCTCGGTTCT CACAAGCGTC ACAGAAGCTT TAGTGTCTAT GATCTTCCCT 
TTCCATTGGC CGTGTCCTTA CATTCCACTT TGCCCTTTGG CCCTGGCAGA CGTACTAAGC 
GCCCCATGCC CCTTCATCGT GGGAATTGAT TCTCGCTACT TTGATCTGTA CGACCCCCCT 
CCGGATGTAA GCTGTGTTGA CCTAGATACC AACACCATCT CTCAAATTGA AGGCAAAAGA 
AATGTAACAT GGAAAATCCT ACCGAAGAAA GCTTGTAAGA ACCTGCTCAA CACTCTTTGT 
AACTTGTACC AGCAACTGGC AGAGTTGCAA CACAGACCAC GGGAAGAAGC TTTTATGGAA 
CTTGCAATGA ATGACTATGA CTTTAACTAT GGAAAAAGAA TGCATCAGCT GGACATGGAG 
ATTCAGGAAG CATTCCTGTG CTTTATGGCC TCTATTTTAA AAGGATACAG ATCGTATCTT 
CGGCCAATTA CCCAAGCACC ATCAGAGACT GCAACAGATG CCAGCTCTCT CTTCGAACTG 
CAAGGTTTCT TAAAAAGTCG TGATCGCTCA CACCACAAGT TTTACAACAT GATGACCAAG 
ACTCAGATGT TCATCCGCTT TATTGAGGAG TGTTCCTTCG TCAGCGATAA GGATGCTAGC 
CTGGCTTTCT TTGATGACTG TGTTGAGAAG GCGGATGTGG ATCGACCAGG AGAGACACGT 
CTTATTGAAC TGGATGAGTC TCACAGAAGT GAGCATACAG TGTTCATCAC ACCACCAGAG 
ATTCCCCACT TACCAAATGG CGAGGAGCCC CCTAAGAACT ATAGCTACAA TGGGTTCCCT 
GTCCTTATCT TGGATCTCTT TGACACGCCA GATGGACTCA TGAAAAGTCA CAAACTTAGT 
ACAAAAGTCA CCACACCCTG CAGTCCTTCT CCAATGTTTA GAAGAACAAA ACAGGAAATC 
CGGTCAGCAC ATAAAATAGC AAAGCAGTAC TCCTCCATCC CCCAGATGTG GTCCAGGTGT 
CTACTGAGAC ATTGCTATGG ACTTTGGTTT ATCTGTCTGC CCGCATATGT GAAATTCTGC 
CATTCCAAAG TGAGAGCACT GCGCACAGCC TACGATGTGT TGCGAAAAAT GCACATGAAA 
AAGATTGACT CTCCTGATGA GGTTTGCTAT AGGGTTCTAA TGCAGTTGTG TGGCCAATAT 
GGGCAGCCTG TCCTTGCTGT TAAAGTTCTC TTTGAAATGA GAAAGGTCGG CGTGCAGCCA 
AATGCTATAA CATATGGCTA TTATAATAAG GCTGTCCTGG AAAGCGTATG GCCCTCTGGG 
AATAGAGGGG GTTACTTTCT GTGGATAAAG GTGAGGAATG TGGTGCTAGC AGTAGCCCAG 
TTCAAACAGG CTTTGATAAA GCAACATGCT TCAGCTCATC AAACTCCTGC TTCAGATTGC 
AGTGATTTGG ACGCAGTCAG CCATGGCAGT TTGGATAGTT CTGTTGACAT GAACCCTACA 
GAACAGCCTT CATTCAGTAC AGATGTAACA TGTGCTGATC CAGCTGACGA CCGATCTAGT 
ACAGGAGGCC AGTCAGACCT TGGCTACAGT TCATTATCAA AAGAAGAAGT AAGAAGAGGA 
GCCGTGCATG ACATACAGGA GGAGAAGGAA GACTCCAGAG ATAGTGACTC CAGTTCGTCA 
TCTGATTCAG AAAGTTCCAA GAGCAGCAGA GACTGTGTGC CACAAGGAAC TGGCAGTCAG 
GGTCAACAAA CATTGGCAAG CACATCTAGT ATAGTCCGGA AAGAAGCAAA ATCAGACAGT 
GCAAGCAGTG ACAACTCAGC AGGTCTACTG TTCACACCAT CCTTTAGTGA GAATTGTGAA 
AGTGAAGTGT ATCAGTGCTG GAGTCTACAT AAAAGACACA AGAGTCCTGT GGAGTCAGAT 
CTGAAAAAAC AGATGGAATG GGAAAAGAGG AACAGGCATC TAAGCAGTGG CAATGTTGAG 
GAATTATTTA TCAGTAAGTC TGATGGACAG AATAACACTG AAAACATGGT GGACAAACTT 
GGAGCAGATG CAAAAATACT TAGCACTGTA TATAAGCAAC ACATGGCACG GCCCAAGACC 
CTGGACTTAA ATAAAATGAA TCCCAGAAAG TCAAAGGAGG TTGTTGAAAA AGAATCTAGT 
GATGATGAGT CTCCCATAGG AGGAGCAGAT GACAAAGTGG ACTCCCCTGT TATTTTTGAC 
CTTGAAGATC TAGATAATGA TTCTGATGAA CTTAAATCAG CAAAACCATG TGTCCGGAAG 
GCCAAACTTG CTCAAAGAAG TATCAGCATG CCCTTTTCAT CTTCCATGTT TCTGGCTAAG 
CCAGCTGAAA AAACAGACGT GAAGGCTGGA TTAGACCCTT TGTCATTGCT GGCAGCTGAA 
ACAGAACAGG CAGAAGTAGA ACAGGAGGAG GAAGATGACA AAAGCTTACT AACACCATCT 
GCTAAAAGGG ACCTTGCAGA GGAAATAGAA ATGTACATGA ACAATATAAG CAGCCCCCTT 
GCAAGTCGAA CCCCTAGCAT GGACCTTGAC AGATCCAGTT CTGAACTAAA TAAAACATGT 
AAAGCTGTAG CACTCAACAG GCGCTTGAGT GTTCCTGCTA GATCCCCACA AGAACCAAGA 
CTAACCAGAT CAATTACTGC CCACAGTAAA CGCGACAAAA GACAAAGAAG CAGGCTCTGG 
TCTTCTCCTG CTGTTTCCGA GAACAATCCT CAGAATGAGC AAAGCCCAGA ATCTGCCCTT 
GCTTTAATGT CCCCAACATC ATTTAACATA GATACACTTC TCACGCCAAC CTTTGATGTT 
CTTAGGAGCA ACATGTTCTC AGCTGGCAAA GGAGTAGCCA AGAAGGCCAG CAAATGGTAT 
TCAAAGATCG CAAATTATGC GGGGCCTGCA AAGGATCAGA ATTCTGACCG AGCCAGTCTG 
TCTTCAGGAG GAGCAGGGGA TACTGAATCC TACTCTTTAA ATGATGATGA TTTTGATGGC 
AGTGCTTCTC TGCATGAGAC TGATGTCTTT CCTGGAACCA ATACCATTGG TGTAAGCAGA 
TGCAGCTTGG AGAGTAGCAC ATCTCAAGAC AGTAACTCTA AACAACTTTA CAATAATGGT 
CTGCCATCAA CAGCATCTCC AAACTACAAG AGTGAGATTC TTTCATCCTG CAATTCCAGT 
AGCACTAGTG TCTTCCAGAA CTATGCTATG GAGGTTCTGA TATCTAGCTG TTCACGTTGC 
TTGACCTGTG ACGGTCTTGT TCATGATGAG GAGATTATGG CAGGCTGGAC GGCAGATGAC 
TCAAACCTTA ACACCACATG TCCTTTTTGC GGGAACTCAT TTCTTCCATC TTTAAACGTT 
GAAATTAGGG ACCTGCGACG CCCTGGAAGG TACTTTCTGA AGGCCAGCCC ATCTGCAGAA 
AGTATGCCGC ATTCTTCAGT ATCCTCACAA AGACCTAACT CAGATATTCT GACTCCGGCT 
TCTCCCTGTG CTTCCACCAA AGTCAAGGAT TTTAGTTTGT GCAGTAAAAC CAAACAAGAG 
AGAACTCGGT CTGTAACTGT TGAGATCCCA GTGGGGAAGA GAACCAGAGA TCTCAATGGG 
TCCTTATGCC CGAATCAGCC CATTGCACGA AGTATCAGCA CATTTGGTCC CTTAGATGAA 
CAGCCATCTC ATGACCAGAG CCATGTTATT GCCACTGGCA GCCTCCCAAC TTGCTTCCAG 
GCACCTCCTG AGTCAGGATT GGAATGGAAG ATGTCAAATC CAGAGCCAAT CACAGTCCCT 
TACCTTAGCC CGCTGGTTGT CTGGAAGGAG CTGGAGAGCC TGCTAGAAAA TGAGGGTGAC 
CAATCGATTA CAGTTTCTGG ATTTGTGGAC CATCATCCGA TAGTATTCTG GAATTTGGTG 
TGGTATTTCA GGAGGCTGGA TCTCCCAAGT AACCTCCCGG GATTAATTCT GTCTTCGGAA 
CAATGCAATA AAAATTCAAA GGTCTCCAGG AGCTGTATGT CTGCAGACAG CAAACATGTC 
CTTATTCAAG TGCTGTGGGA CAATCTGAAA TTGCACCAGG ATCCCCGGCA GCCTCTCTAC 
ATCCTCTGGA ACACTATTAC ACAGGAACAT GCGCTTGCTG TGCCACAGAT CAAAGAGGAG 
GAATTGTTTG ATCAGCAGTT TCTCAGAGGG ATAGTAAGAA GCATAAAGAA CAACAATGTT 
TATGGTCCTG TGAGCCAGAT ATTGGACTGT ATTAAACAGG TCCCTGGAAG AACGAGGCAG 
AGGAGTCTGT ACAGGGAAAT TCTTTTTCTT TCTCTTGTTG CCTTGGGAAG AGAGAATATC 
GATATTGATG TATTTGATCG GGAATATAAA ACTGCATACG AGCGCCTCAC CCCTGAGCAG 
CTGAACTGCA CACAGATTTG TGATCGGCCT CCCAGCATTG GAGTGATGGA GTGCAGGAAG 
ACTTTCGGAC AGCCATTCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947990.2
CDS	48..5831
Translation	MGTFEEEQVSSCPTPKRKMMEDKGPRVADYFVVTGLTDSSKPLEEEIHFNDACHKTAKPKEPITDVTVIIKSLGEEVPQGYVCIDLTPTGLSADLNNGSIRGPQIYLCYRRGRDKPPLTDLGVLYEWKERLKQGCKIIQATPYGRPANISGSASSQRVFITYRRAAESMTQNSLAVTDICIIIPSKGETPPHTFCKVEKNLNSSVWGSAVYLCYKKSVAKTNAVSYKAGLICRYPEEDYESFPLPESVPLFCLPMGATIECWPSDSNYPLPVFSTFVLTGASAEKVYGAAIQFYEPYAQDQLTEKQQVHLGLLSSAEGQMDSSKSIHVNKCICLLSHWPFFDAFKKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDTLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQIEGKRNVTWKILPKKACKNLLNTLCNLYQQLAELQHRPREEAFMELAMNDYDFNYGKRMHQLDMEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFELQGFLKSRDRSHHKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVEKADVDRPGETRLIELDESHRSEHTVFITPPEIPHLPNGEEPPKNYSYNGFPVLILDLFDTPDGLMKSHKLSTKVTTPCSPSPMFRRTKQEIRSAHKIAKQYSSIPQMWSRCLLRHCYGLWFICLPAYVKFCHSKVRALRTAYDVLRKMHMKKIDSPDEVCYRVLMQLCGQYGQPVLAVKVLFEMRKVGVQPNAITYGYYNKAVLESVWPSGNRGGYFLWIKVRNVVLAVAQFKQALIKQHASAHQTPASDCSDLDAVSHGSLDSSVDMNPTEQPSFSTDVTCADPADDRSSTGGQSDLGYSSLSKEEVRRGAVHDIQEEKEDSRDSDSSSSSDSESSKSSRDCVPQGTGSQGQQTLASTSSIVRKEAKSDSASSDNSAGLLFTPSFSENCESEVYQCWSLHKRHKSPVESDLKKQMEWEKRNRHLSSGNVEELFISKSDGQNNTENMVDKLGADAKILSTVYKQHMARPKTLDLNKMNPRKSKEVVEKESSDDESPIGGADDKVDSPVIFDLEDLDNDSDELKSAKPCVRKAKLAQRSISMPFSSSMFLAKPAEKTDVKAGLDPLSLLAAETEQAEVEQEEEDDKSLLTPSAKRDLAEEIEMYMNNISSPLASRTPSMDLDRSSSELNKTCKAVALNRRLSVPARSPQEPRLTRSITAHSKRDKRQRSRLWSSPAVSENNPQNEQSPESALALMSPTSFNIDTLLTPTFDVLRSNMFSAGKGVAKKASKWYSKIANYAGPAKDQNSDRASLSSGGAGDTESYSLNDDDFDGSASLHETDVFPGTNTIGVSRCSLESSTSQDSNSKQLYNNGLPSTASPNYKSEILSSCNSSSTSVFQNYAMEVLISSCSRCLTCDGLVHDEEIMAGWTADDSNLNTTCPFCGNSFLPSLNVEIRDLRRPGRYFLKASPSAESMPHSSVSSQRPNSDILTPASPCASTKVKDFSLCSKTKQERTRSVTVEIPVGKRTRDLNGSLCPNQPIARSISTFGPLDEQPSHDQSHVIATGSLPTCFQAPPESGLEWKMSNPEPITVPYLSPLVVWKELESLLENEGDQSITVSGFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEQCNKNSKVSRSCMSADSKHVLIQVLWDNLKLHQDPRQPLYILWNTITQEHALAVPQIKEEELFDQQFLRGIVRSIKNNNVYGPVSQILDCIKQVPGRTRQRSLYREILFLSLVALGRENIDIDVFDREYKTAYERLTPEQLNCTQICDRPPSIGVMECRKTFGQPFL

Target ORF information:

RefSeq Version	XM_018092501.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DENN domain containing 4A (dennd4a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018092501.2

ORF Insert Sequence:

ATGGGAACTT TTGAGGAAGA GCAAGTAAGC AGTTGTCCTA CTCCAAAACG GAAGATGATG 
GAAGACAAAG GGCCCCGTGT GGCTGACTAC TTTGTAGTTA CAGGACTGAC AGATTCATCA 
AAACCACTTG AGGAGGAGAT CCACTTTAAT GATGCCTGTC ATAAAACAGC TAAACCAAAG 
GAACCCATTA CAGATGTGAC TGTGATAATC AAATCCTTAG GGGAGGAAGT GCCACAGGGA 
TATGTATGCA TAGACCTTAC CCCAACTGGA CTATCAGCCG ATCTCAATAA TGGAAGTATT 
CGAGGGCCAC AGATATATCT TTGTTACAGG AGAGGAAGAG ACAAACCACC TCTAACAGAT 
CTTGGGGTAT TGTACGAATG GAAGGAAAGA TTAAAACAGG GCTGCAAGAT TATTCAGGCT 
ACTCCATATG GCCGTCCAGC TAATATAAGC GGTAGTGCAT CCTCACAGAG AGTATTTATC 
ACATACCGCA GAGCTGCTGA GAGCATGACC CAGAATTCCT TGGCTGTAAC TGATATCTGC 
ATCATTATAC CAAGCAAAGG AGAGACTCCA CCACACACAT TTTGCAAAGT GGAAAAGAAT 
CTGAACAGCA GCGTGTGGGG CTCTGCTGTG TACCTGTGCT ACAAGAAATC AGTTGCCAAA 
ACAAATGCAG TATCCTATAA AGCTGGGTTG ATATGCAGGT ATCCAGAGGA GGATTATGAA 
TCATTTCCTC TGCCGGAATC AGTCCCACTA TTTTGTCTAC CCATGGGGGC AACAATTGAA 
TGTTGGCCAT CTGACAGTAA TTATCCACTC CCTGTTTTTT CCACTTTTGT TTTAACTGGA 
GCATCAGCTG AAAAGGTGTA TGGAGCTGCT ATTCAGTTTT ATGAGCCATA TGCTCAAGAT 
CAGCTGACTG AAAAGCAACA AGTACATTTA GGCCTCTTGA GCAGTGCAGA GGGTCAGATG 
GACTCTTCTA AAAGTATCCA TGTCAACAAG TGCATCTGCC TGCTGTCCCA CTGGCCTTTT 
TTTGATGCTT TCAAGAAATT TCTCACTTTC CTTTACCGCT ATTCAATATC GGGTCCTCAT 
GTGCTGCCAA TAGAAAAACA TATCTCCCAC TTCATGCACA AAGTCCCATT CCCATCTCCA 
CAGAGACCAC GCATTCTAGT TCAGCTTTCC CCGCATGATA CTCTGATCCT AAGCCAGCCT 
GTGTCCTCAC CACTTCCACT GAGTGGTGGA AAGTTTTCAA CGTTGCTGCA GAATTTGGGA 
CCTGAAAATG CTGTGACTCT GCTTGTATTT GCTGTCACGG AGCATAAGAT TCTGATCCAT 
TCGTTGCGCC CCTCGGTTCT CACAAGCGTC ACAGAAGCTT TAGTGTCTAT GATCTTCCCT 
TTCCATTGGC CGTGTCCTTA CATTCCACTT TGCCCTTTGG CCCTGGCAGA CGTACTAAGC 
GCCCCATGCC CCTTCATCGT GGGAATTGAT TCTCGCTACT TTGATCTGTA CGACCCCCCT 
CCGGATGTAA GCTGTGTTGA CCTAGATACC AACACCATCT CTCAAATTGA AGGCAAAAGA 
AATGTAACAT GGAAAATCCT ACCGAAGAAA GCTTGTAAGA ACCTGCTCAA CACTCTTTGT 
AACTTGTACC AGCAACTGGC AGAGTTGCAA CACAGACCAC GGGAAGAAGC TTTTATGGAA 
CTTGCAATGA ATGACTATGA CTTTAACTAT GGAAAAAGAA TGCATCAGCT GGACATGGAG 
ATTCAGGAAG CATTCCTGTG CTTTATGGCC TCTATTTTAA AAGGATACAG ATCGTATCTT 
CGGCCAATTA CCCAAGCACC ATCAGAGACT GCAACAGATG CCAGCTCTCT CTTCGAACTG 
CAAGGTTTCT TAAAAAGTCG TGATCGCTCA CACCACAAGT TTTACAACAT GATGACCAAG 
ACTCAGATGT TCATCCGCTT TATTGAGGAG TGTTCCTTCG TCAGCGATAA GGATGCTAGC 
CTGGCTTTCT TTGATGACTG TGTTGAGAAG GCGGATGTGG ATCGACCAGG AGAGACACGT 
CTTATTGAAC TGGATGAGTC TCACAGAAGT GAGCATACAG TGTTCATCAC ACCACCAGAG 
ATTCCCCACT TACCAAATGG CGAGGAGCCC CCTAAGAACT ATAGCTACAA TGGGTTCCCT 
GTCCTTATCT TGGATCTCTT TGACACGCCA GATGGACTCA TGAAAAGTCA CAAACTTAGT 
ACAAAAGTCA CCACACCCTG CAGTCCTTCT CCAATGTTTA GAAGAACAAA ACAGGAAATC 
CGGTCAGCAC ATAAAATAGC AAAGCAGTAC TCCTCCATCC CCCAGATGTG GTCCAGGTGT 
CTACTGAGAC ATTGCTATGG ACTTTGGTTT ATCTGTCTGC CCGCATATGT GAAATTCTGC 
CATTCCAAAG TGAGAGCACT GCGCACAGCC TACGATGTGT TGCGAAAAAT GCACATGAAA 
AAGATTGACT CTCCTGATGA GGTTTGCTAT AGGGTTCTAA TGCAGTTGTG TGGCCAATAT 
GGGCAGCCTG TCCTTGCTGT TAAAGTTCTC TTTGAAATGA GAAAGGTCGG CGTGCAGCCA 
AATGCTATAA CATATGGCTA TTATAATAAG GCTGTCCTGG AAAGCGTATG GCCCTCTGGG 
AATAGAGGGG GTTACTTTCT GTGGATAAAG GTGAGGAATG TGGTGCTAGC AGTAGCCCAG 
TTCAAACAGG CTTTGATAAA GCAACATGCT TCAGCTCATC AAACTCCTGC TTCAGATTGC 
AGTGATTTGG ACGCAGTCAG CCATGGCAGT TTGGATAGTT CTGTTGACAT GAACCCTACA 
GAACAGCCTT CATTCAGTAC AGATGTAACA TGTGCTGATC CAGCTGACGA CCGATCTAGT 
ACAGGAGGCC AGTCAGACCT TGGCTACAGT TCATTATCAA AAGAAGAAGT AAGAAGAGGA 
GCCGTGCATG ACATACAGGA GGAGAAGGAA GACTCCAGAG ATAGTGACTC CAGTTCGTCA 
TCTGATTCAG AAAGTTCCAA GAGCAGCAGA GACTGTGTGC CACAAGGAAC TGGCAGTCAG 
GGTCAACAAA CATTGGCAAG CACATCTAGT ATAGTCCGGA AAGAAGCAAA ATCAGACAGT 
GCAAGCAGTG ACAACTCAGC AGGTCTACTG TTCACACCAT CCTTTAGTGA GAATTGTGAA 
AGTGAAGTGT ATCAGTGCTG GAGTCTACAT AAAAGACACA AGAGTCCTGT GGAGTCAGAT 
CTGAAAAAAC AGATGGAATG GGAAAAGAGG AACAGGCATC TAAGCAGTGG CAATGTTGAG 
GAATTATTTA TCAGTAAGTC TGATGGACAG AATAACACTG AAAACATGGT GGACAAACTT 
GGAGCAGATG CAAAAATACT TAGCACTGTA TATAAGCAAC ACATGGCACG GCCCAAGACC 
CTGGACTTAA ATAAAATGAA TCCCAGAAAG TCAAAGGAGG TTGTTGAAAA AGAATCTAGT 
GATGATGAGT CTCCCATAGG AGGAGCAGAT GACAAAGTGG ACTCCCCTGT TATTTTTGAC 
CTTGAAGATC TAGATAATGA TTCTGATGAA CTTAAATCAG CAAAACCATG TGTCCGGAAG 
GCCAAACTTG CTCAAAGAAG TATCAGCATG CCCTTTTCAT CTTCCATGTT TCTGGCTAAG 
CCAGCTGAAA AAACAGACGT GAAGGCTGGA TTAGACCCTT TGTCATTGCT GGCAGCTGAA 
ACAGAACAGG CAGAAGTAGA ACAGGAGGAG GAAGATGACA AAAGCTTACT AACACCATCT 
GCTAAAAGGG ACCTTGCAGA GGAAATAGAA ATGTACATGA ACAATATAAG CAGCCCCCTT 
GCAAGTCGAA CCCCTAGCAT GGACCTTGAC AGATCCAGTT CTGAACTAAA TAAAACATGT 
AAAGCTGTAG CACTCAACAG GCGCTTGAGT GTTCCTGCTA GATCCCCACA AGAACCAAGA 
CTAACCAGAT CAATTACTGC CCACAGTAAA CGCGACAAAA GACAAAGAAG CAGGCTCTGG 
TCTTCTCCTG CTGTTTCCGA GAACAATCCT CAGAATGAGC AAAGCCCAGA ATCTGCCCTT 
GCTTTAATGT CCCCAACATC ATTTAACATA GATACACTTC TCACGCCAAC CTTTGATGTT 
CTTAGGAGCA ACATGTTCTC AGCTGGCAAA GGAGTAGCCA AGAAGGCCAG CAAATGGTAT 
TCAAAGATCG CAAATTATGC GGGGCCTGCA AAGGATCAGA ATTCTGACCG AGCCAGTCTG 
TCTTCAGGAG GAGCAGGGGA TACTGAATCC TACTCTTTAA ATGATGATGA TTTTGATGGC 
AGTGCTTCTC TGCATGAGAC TGATGTCTTT CCTGGAACCA ATACCATTGG TGTAAGCAGA 
TGCAGCTTGG AGAGTAGCAC ATCTCAAGAC AGTAACTCTA AACAACTTTA CAATAATGGT 
CTGCCATCAA CAGCATCTCC AAACTACAAG AGTGAGATTC TTTCATCCTG CAATTCCAGT 
AGCACTAGTG TCTTCCAGAA CTATGCTATG GAGGTTCTGA TATCTAGCTG TTCACGTTGC 
TTGACCTGTG ACGGTCTTGT TCATGATGAG GAGATTATGG CAGGCTGGAC GGCAGATGAC 
TCAAACCTTA ACACCACATG TCCTTTTTGC GGGAACTCAT TTCTTCCATC TTTAAACGTT 
GAAATTAGGG ACCTGCGACG CCCTGGAAGG TACTTTCTGA AGGCCAGCCC ATCTGCAGAA 
AGTATGCCGC ATTCTTCAGT ATCCTCACAA AGACCTAACT CAGATATTCT GACTCCGGCT 
TCTCCCTGTG CTTCCACCAA AGTCAAGGAT TTTAGTTTGT GCAGTAAAAC CAAACAAGAG 
AGAACTCGGT CTGTAACTGT TGAGATCCCA GTGGGGAAGA GAACCAGAGA TCTCAATGGG 
TCCTTATGCC CGAATCAGCC CATTGCACGA AGTATCAGCA CATTTGGTCC CTTAGATGAA 
CAGCCATCTC ATGACCAGAG CCATGTTATT GCCACTGGCA GCCTCCCAAC TTGCTTCCAG 
GCACCTCCTG AGTCAGGATT GGAATGGAAG ATGTCAAATC CAGAGCCAAT CACAGTCCCT 
TACCTTAGCC CGCTGGTTGT CTGGAAGGAG CTGGAGAGCC TGCTAGAAAA TGAGGGTGAC 
CAATCGATTA CAGTTTCTGG ATTTGTGGAC CATCATCCGA TAGTATTCTG GAATTTGGTG 
TGGTATTTCA GGAGGCTGGA TCTCCCAAGT AACCTCCCGG GATTAATTCT GTCTTCGGAA 
CAATGCAATA AAAATTCAAA GGTCTCCAGG AGCTGTATGT CTGCAGACAG CAAACATGTC 
CTTATTCAAG TGCTGTGGGA CAATCTGAAA TTGCACCAGG ATCCCCGGCA GCCTCTCTAC 
ATCCTCTGGA ACACTATTAC ACAGGAACAT GCGCTTGCTG TGCCACAGAT CAAAGAGGAG 
GAATTGTTTG ATCAGCAGTT TCTCAGAGGG ATAGTAAGAA GCATAAAGAA CAACAATGTT 
TATGGTCCTG TGAGCCAGAT ATTGGACTGT ATTAAACAGG TCCCTGGAAG AACGAGGCAG 
AGGAGTCTGT ACAGGGAAAT TCTTTTTCTT TCTCTTGTTG CCTTGGGAAG AGAGAATATC 
GATATTGATG TATTTGATCG GGAATATAAA ACTGCATACG AGCGCCTCAC CCCTGAGCAG 
CTGAACTGCA CACAGATTTG TGATCGGCCT CCCAGCATTG GAGTGATGGA GTGCAGGAAG 
ACTTTCGGAC AGCCATTCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	dennd4a
Entrez Gene ID	100490151
Full Name	DENN domain containing 4A
General protein information	Preferred Names DENN domain containing 4A Names C-myc promoter-binding protein DENN/MADD domain containing 4A
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Homo sapiens (human)	DENND4A	NP_001138295.1
Bos taurus (cattle)	DENND4A	NP_001192454.1
Gallus gallus (chicken)	DENND4A	XP_413911.4
Drosophila melanogaster (fruit fly)	Crag	NP_727281.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001102	XP_322065.3
Pan troglodytes (chimpanzee)	DENND4A	XP_523102.2
	DENND4A	XP_001109950.1
Rattus norvegicus (Norway rat)	Dennd4a	XP_006243364.1
Mus musculus (house mouse)	Dennd4a	NP_001156389.1
Canis lupus familiaris (dog)	DENND4A	XP_005638584.1
Danio rerio (zebrafish)	dennd4a	NP_001074458.1
Xenopus tropicalis (tropical clawed frog)	dennd4a	XP_004912733.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

dennd4a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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