ubr1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ubr1
Entrez Gene ID	100490039
Full Name	ubiquitin protein ligase E3 component n-recognin 1
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 1 Names E3 ubiquitin-protein ligase UBR1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002941086.4	XP_002941132.2	E3 ubiquitin-protein ligase UBR1 isoform X1
XM_012969237.2	XP_012824691.1	E3 ubiquitin-protein ligase UBR1 isoform X2
XM_002941086.5	XP_002941132.3	E3 ubiquitin-protein ligase UBR1 isoform X1
XM_012969237.3	XP_012824691.2	E3 ubiquitin-protein ligase UBR1 isoform X2
XM_012969238.2	XP_012824692.1	E3 ubiquitin-protein ligase UBR1 isoform X3
XM_012969238.3	XP_012824692.2	E3 ubiquitin-protein ligase UBR1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBR1	XP_510341.3
	UBR1	XP_001105721.2
Canis lupus familiaris (dog)	UBR1	XP_544645.2
Bos taurus (cattle)	UBR1	NP_001192826.1
Danio rerio (zebrafish)	ubr1	XP_002665264.3
Xenopus tropicalis (tropical clawed frog)	ubr1	XP_002941132.2
Rattus norvegicus (Norway rat)	Ubr1	NP_001171543.1
Homo sapiens (human)	UBR1	NP_777576.1
Mus musculus (house mouse)	Ubr1	NP_033487.2
Gallus gallus (chicken)	UBR1	XP_421165.3
Schizosaccharomyces pombe (fission yeast)	ubr1	NP_596158.2
Magnaporthe oryzae (rice blast fungus)	MGG_13171	XP_003710667.1
Neurospora crassa	NCU03234	XP_965334.2

Gene Ontology

The following ubr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ubr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa65687	XM_002941086.5 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa65688	XM_012969237.3 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa65689	XM_012969238.3 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa14964	XM_002941086.4 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa34672	XM_012969237.2 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa34673	XM_012969238.2 Latest version!	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ubr1 ( XM_002941086.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941132.3 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X1, mRNA.

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CloneID	OXa65687
Clone ID Related Accession (Same CDS sequence)	XM_002941086.5
Accession Version	XM_002941086.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002941086.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCTG GAGGTGGAGG ATTGGAGCAA AGCAGGGAGC CAGGGGATGG AGAAATGCTT 
GCCATGAGAT TAGTCAACGC TTTGGATTTT GCCTCTGCCT TTCACCAACA CGTGGCAAAG 
CATGTCCCCC AGATTTATAC AGATGAGCTG CAGCCACAAG TGGAGAAGCA AGAGGAGCTG 
GCACAGAAAC TTATTTTAAC CCCACTGGAG TGGTGCCTCT TTAGAGAAGA TCCAAATGTA 
TTCAAGGAGA AGTTACGGCA CAGTGCAGCT CCTCCATTAT GTGGAAGAGT CTTTAGAGGA 
GGGGAGACAA CCTATTCCTG CAGGGATTGT GCCATTGATC CAACCTGTGT CCTGTGTATG 
GATTGTTTCC AGAACAGCAT TCATAAAAAT CACAGATACA AGATGCACTC TTCAATGGGA 
GGGGGATTTT GTGACTGTGG GGATACTGAA GCCTGGAAAA CCGGCCCATA TTGTAAGATC 
CATGAACCAG GGCCATCAGA TCAGAATTCA GACTTTCGTT TGAAGGATGA ACTTCTGGCC 
CAAACCAAAA TAGTATTCCC TTTGATTATC CGGTATATAG TAGACATGTT AATCTGGGAA 
GAACAAAAGA GTTTGCCAGC ATATCTGGAT ACAGGGACAG TGGAGTGGAA CCAGTACTAC 
TGTGTACTTT ACAATGATGA ACACCACACC TATGATCACT TCATCTACAG TTTGCAGAGA 
TCGCTTGGTT GTGATCAGAA AGAAGCACAG ACATTAACCA GTGCAGCTGA GAGAGAGGGC 
CGCAAAGCTG TTAAAAAGGG TACTTTAGAG CAATGCCAGG AGGTGACAGA AAGTCTAAAG 
GCTAATTCAG AGAACGTGTC GCTGAAACCT GTCCACGTGG AGGTGCTGCA TGGTCATGTA 
ATGGCCCATC AAAGCTTTGC ACTGTGTTTA GCAATCTGGC TTAACAAACT CCTGGCCTAC 
TCTAGTGACT TCCGACAGAT CTTCTGTCAA GTGTGCCTAG AGCAAGAGCC AAAGCCTAGC 
AGTTTTAATC ACACTTGTTA TGTCAGCTTG TTAATTTTAA AGGACTCCAA GCTTCATAAA 
GGAGTGCGGA AAGTTCTGCA TGATCTGATC TTCAGCAGCT TTTTCATGGA AATGGAGTAT 
AAGAAACAAT TTGCTATAGA GTTTGTTAAG CATTACAAGC AGATACAGAA AGAATACATA 
AACGACGATC AGGACAGGAA CATTTCAGTT ACTGCAATAT CAGTTCAGGT TTTCACTGTC 
CCTACTTTGG CTCGCCTACT GATAGAAGAA CAAAATGTAA TGAAAGTCAT CACAGAGACT 
TTACTCGAGG TCATAAAAGA ATATCTTGGC TCAGACAACA AGTTCAACTT CCAGGGCTAC 
AGCCAGGAAC GCTTTTTCAT GGTATATGCC ATAATCCTTG ACCTCAAGTA TATCCTTATC 
TCTAAACCTA CATCCTGGTC TGAGAAGTTA AAAGCCAAGT TTCTGGAAGG ATTTAAATCT 
TTCCTCTATT TTTTATCCTG TATGCAGGGC ATGGAAGGGA TAAGCAGGAT GGTGGGACAG 
CACGTTGAGA TGGATTCAGA CTGGGAAGCT GTAATAGCTA TGCAGATGCA ACTGAAAAAT 
GTCTTGACGC TGATACAGGA ATGGTGTGCA ACAGATGATG ATATATTGCT CAAATCCTAC 
AAGGAATGCC ATCTGAGTCT TCTGCAGTGT TGCACCCCGG TCCCTTCCCA AGAAAAGAAG 
CTGATAAAAA TGAGTGGCCA TTCATACCAA AGCTATCCAT ACATGGTGTC CAAAGACCCA 
ATCAGCATAC ACTTACCTCT CTCTCGGATG CTTGCTGGTC TACATGCACA GATGTGTAGG 
ACTGGTGTCA TCACAAGACT GTGTGAAATT CTTCCTCCGG AAGACTTACA GGTGGAACTA 
ATGATTGAAC ACCCCTTAAG ATGCCTGGTG CTGGTGGGAC AGGTAGCAGC TGAGATGTGG 
AGAAGGAATG GCCTTACTAT TGTTAGTCAG GTTTTTTACT ATCATGATGT GAAGTGCAGG 
GAGGAGATGT ATGACAAAGA TGTTGTGATG CTACAGATTG GTGCATCCTA CCTGGATCCC 
AACAGCTTCC TACTGCTCTT GCTGCAGAGG TATGAACTGG AGGAGGCATT CTCTAATGTA 
GCTGTGCCCA CCAAAGACAT GGATGTAGCA AGGCAACACA ACATCTTAAT TGAAGAAATG 
TTACATGTCC TTATTTGTGT TATTGGTGAG CGCTATGTGC CAGGCATTAG CAATGTTACT 
AGAGAAGAGT GCACTATGAG GGAGATCATC CATCTGCTTT GTATTGAACC TATGGCTCAC 
AGTGCAATCG CCAAGGCTTT GCCAGAGACT GAAAACACTG ACATATGCCT GGATAAACTC 
ATCAGTAAAG TTGCCACTTT TAAAAAACCG GGAGTATCAG GACATGGCGT TTATGAACTA 
AAGGCTGAAT ACCTCGATCA ATTTAATGTG TTCTTTTATC ATTACACCAA AACACAACAC 
AGCAAGGCAG AACACACACA GAAGAAAAGG CGAAAACAAG AAAACAGCTC AGAAGCATTA 
GCCCCTCCTG CACCCCCCAA TTTCAGCCCA GCTTTTTGCA ACGTGACCAA GCTTCTGTGC 
TGTGATATTA TGATGCATAT TCTACGAACC ATCTTGAAGA GAGCAGCAGA GGAAGATCCA 
ATTATGTGGA CAGAAGGCAT GATACAAATG GTCCTCCATA TTATAGCCCT GGGGTTGTTA 
GAAGAGAAGC AACAGCTACA GAGGGCTGCT GAAGAAGAAG TAACATTTAA CTTCTATCAT 
AAGGCAACAA GGGTTAGGAG TACAGATGCA AATGCACAAA GTGTTGCGAC ACTGCTGGAG 
AGGCTGAAGG GCATTACCCA GCTGGATGCC CAGAAAGACA TGATCATTTG GGTGCGACAA 
GTTTTTGAAA CCGTAAAAGG GCTGAGAGAG ACATCTAGTC ACACAGTTCC CACAGATACA 
GTTTCTGACG TTGCAAAAGC TGAAGAGGGA CAAAGCCCCC AGGAAAAGGA GAAGCGCAAA 
AGAAAAGCTG AGGCGGCCCG GCTCCACCGC CAGAAGATCA TGGCACAGAT GTCAGCTTTA 
CAAAAAAATT TCATTGAAAC AAATAAGCTT CTGTATGACA CCACTGTGGA AGTACAAGAA 
AGCACTAGTC CTGTAGAAGA GAGCCTGTTA TCAGCAGATG AAGGATCGAA GGTTGCCTTG 
GGGACAAAAC GTGGCCCCAC AGTGCCTAGC AAAGATGTTC TAACATGTAT TCTATGTCAA 
GAAGAACAGG AAGTGAAATT GGACAAGCCT ACAATGGTGC TAACTGCCTG CGTCCAAAAG 
TCCACAACAC TGTCCCAAAC TCGTAGCAAA GTGGTCTCAC ACACAGGAGA AACGTTTGAT 
CCACTTTTTA CACACCCAGA GATGTTGTAT GGAACCCATA CAGGCAGCTG TGGCCATGTA 
ATGCATGTTG TGTGCTGGCA GAAGTTCTTT GAGGCAATGC AGAAGAATAC ACGGCAGCGT 
CTTCACGTGG AGCTAATTTT TGATCTTGGA AATGGCGAAT ATCTTTGTCC GCTATGCAAA 
TGCCACTGTA ACACCGTTAT ACCTATAATC CCTCATGTTG CCCAGCAAAT ACAAAGTGTG 
GATGCTGAAG CCATAAATTG TGTTTTCTCA CTTCCTCATT GGTTGGACAT AGTGGCGGCC 
AGAATATCTG GTTATAATTT AAACACCATC AAAGGAACAA AGCCAATCAC TCCTGAATTC 
TGTAAAATTT ATAAAGACCA GAATTATGAG TTCCGTTCCA TCTTGAGCTT TGGCGTGCAG 
GCTCCGCCCC GCTATCCAAC TTGCACAGAA GGAATGCTTG TTCACTTTGC CACTACAGTG 
TACCGCGTGG GCTTAAATGT TCCTCCCAAT GAGTCTGATT CACGTATGCC TGTCATGACA 
TGGAATACTT GTGCATTTAC AATCCAGTGT ATAGAAAGCT GGCTTCAGGA TGAAGAAAAA 
CCCTTGTTTG GATCCCTGCA AAACCGGCAA CTTGCTGGAT TACAAGCCTT GGTGAAATTT 
GCTGAGGCCC AAAGAGTTAC ATGCCCTCAG CCTTTGATAC GGAGACATCT TATCCGGTTA 
TTAGCAGCAT TCCTGCCAAA CATTACCCTT GAAGACACCC CATCCTTTCT GTCTTTGGAT 
GTTTTCCATC TGCTGGTGGG GTCAGTGTTG GCATTCCCAT CATTATATTG TGAAGAAAAT 
GTAGACTTAC ATCCTTCTAC CCTTGTTTCT ACCTACAACA ACCTCTACCT CTTTCACCTG 
CTCACAATGA CGCACATACT GCAAATCATT TTCTCTTTAA CTACAGATAA TGTGCCTTCT 
ACAGAACCTG ACGACTGTGA AGAAGCCCGT ACAGCTGCTA CTCTTTGTCA GGACATTTCA 
CAACTCACCA GCAACAGTTT AAAGATTGAT TTTCCGGGGT GGTATGTTTG GAAATGTGTG 
AAAGAGGGCA TCATGCCATA TCTGCGATGT GCAGCTTTGT TCTTCCATTA TTTGCTGGGA 
GTAATGCCTC CAGAGGAGCT AAAATCATCA GGCACTACAG ACAATCAGTT ATCAGCTCTT 
TGCAGCTTCC TCTGCTTGCC AACCAACATT CTTCTTCTTT TCCAAGAGAA CTGGGACATT 
GTGAAACTTC TCATTCAGAG GTTGAGTTCT GACCAATCCC TGCAGAGTGT CCTGGCTGGT 
TACACTACTG CACTCAGGTA TCCAAGGAAA CGCAATACAC TAATAGAGCT TCCAGAAGAT 
TACAGTTCCC TTCTCAACCA AGCATCCCAG TTTAGATGTC CTAAGTTTCA AGATGCTGAA 
CGGAAACACC CGGTGATGTG CCTTCTCTGT GGGGCCATTC TCTGTTCTCA GAATACATGC 
TGTCAGGAGG TGGTAAATGG AGAAGAGCTG GGAGCTTGTA CAGCACATGC CATGCACTGT 
GGAGCTGGTG TCTGCATCTT CCTAAGTATC AGAGAATGCA AAGTGGTCCT AATGGAGGGT 
AAAACCAGAG GATGTATCTA CCAGGCTCCC TATCTTGATG AATATGGAGA AACAGACCCA 
GGACTGAAAC GTGGAAACCC CTTACACCTT TCCCATGAGC GATATCGGAA ATTGCATCAG 
TTGTGGCAGC AGCACTGTAT TATTGAAGAG ATTGGACACA ACCTTGAAAT CAACCAGATG 
TTTTTTGACT TTAATTGGAG CCTGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941132.3
CDS	484..5733
Translation	MESGGGGLEQSREPGDGEMLAMRLVNALDFASAFHQHVAKHVPQIYTDELQPQVEKQEELAQKLILTPLEWCLFREDPNVFKEKLRHSAAPPLCGRVFRGGETTYSCRDCAIDPTCVLCMDCFQNSIHKNHRYKMHSSMGGGFCDCGDTEAWKTGPYCKIHEPGPSDQNSDFRLKDELLAQTKIVFPLIIRYIVDMLIWEEQKSLPAYLDTGTVEWNQYYCVLYNDEHHTYDHFIYSLQRSLGCDQKEAQTLTSAAEREGRKAVKKGTLEQCQEVTESLKANSENVSLKPVHVEVLHGHVMAHQSFALCLAIWLNKLLAYSSDFRQIFCQVCLEQEPKPSSFNHTCYVSLLILKDSKLHKGVRKVLHDLIFSSFFMEMEYKKQFAIEFVKHYKQIQKEYINDDQDRNISVTAISVQVFTVPTLARLLIEEQNVMKVITETLLEVIKEYLGSDNKFNFQGYSQERFFMVYAIILDLKYILISKPTSWSEKLKAKFLEGFKSFLYFLSCMQGMEGISRMVGQHVEMDSDWEAVIAMQMQLKNVLTLIQEWCATDDDILLKSYKECHLSLLQCCTPVPSQEKKLIKMSGHSYQSYPYMVSKDPISIHLPLSRMLAGLHAQMCRTGVITRLCEILPPEDLQVELMIEHPLRCLVLVGQVAAEMWRRNGLTIVSQVFYYHDVKCREEMYDKDVVMLQIGASYLDPNSFLLLLLQRYELEEAFSNVAVPTKDMDVARQHNILIEEMLHVLICVIGERYVPGISNVTREECTMREIIHLLCIEPMAHSAIAKALPETENTDICLDKLISKVATFKKPGVSGHGVYELKAEYLDQFNVFFYHYTKTQHSKAEHTQKKRRKQENSSEALAPPAPPNFSPAFCNVTKLLCCDIMMHILRTILKRAAEEDPIMWTEGMIQMVLHIIALGLLEEKQQLQRAAEEEVTFNFYHKATRVRSTDANAQSVATLLERLKGITQLDAQKDMIIWVRQVFETVKGLRETSSHTVPTDTVSDVAKAEEGQSPQEKEKRKRKAEAARLHRQKIMAQMSALQKNFIETNKLLYDTTVEVQESTSPVEESLLSADEGSKVALGTKRGPTVPSKDVLTCILCQEEQEVKLDKPTMVLTACVQKSTTLSQTRSKVVSHTGETFDPLFTHPEMLYGTHTGSCGHVMHVVCWQKFFEAMQKNTRQRLHVELIFDLGNGEYLCPLCKCHCNTVIPIIPHVAQQIQSVDAEAINCVFSLPHWLDIVAARISGYNLNTIKGTKPITPEFCKIYKDQNYEFRSILSFGVQAPPRYPTCTEGMLVHFATTVYRVGLNVPPNESDSRMPVMTWNTCAFTIQCIESWLQDEEKPLFGSLQNRQLAGLQALVKFAEAQRVTCPQPLIRRHLIRLLAAFLPNITLEDTPSFLSLDVFHLLVGSVLAFPSLYCEENVDLHPSTLVSTYNNLYLFHLLTMTHILQIIFSLTTDNVPSTEPDDCEEARTAATLCQDISQLTSNSLKIDFPGWYVWKCVKEGIMPYLRCAALFFHYLLGVMPPEELKSSGTTDNQLSALCSFLCLPTNILLLFQENWDIVKLLIQRLSSDQSLQSVLAGYTTALRYPRKRNTLIELPEDYSSLLNQASQFRCPKFQDAERKHPVMCLLCGAILCSQNTCCQEVVNGEELGACTAHAMHCGAGVCIFLSIRECKVVLMEGKTRGCIYQAPYLDEYGETDPGLKRGNPLHLSHERYRKLHQLWQQHCIIEEIGHNLEINQMFFDFNWSLL

Target ORF information:

RefSeq Version	XM_002941086.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002941086.5

ORF Insert Sequence:

ATGGAGTCTG GAGGTGGAGG ATTGGAGCAA AGCAGGGAGC CAGGGGATGG AGAAATGCTT 
GCCATGAGAT TAGTCAACGC TTTGGATTTT GCCTCTGCCT TTCACCAACA CGTGGCAAAG 
CATGTCCCCC AGATTTATAC AGATGAGCTG CAGCCACAAG TGGAGAAGCA AGAGGAGCTG 
GCACAGAAAC TTATTTTAAC CCCACTGGAG TGGTGCCTCT TTAGAGAAGA TCCAAATGTA 
TTCAAGGAGA AGTTACGGCA CAGTGCAGCT CCTCCATTAT GTGGAAGAGT CTTTAGAGGA 
GGGGAGACAA CCTATTCCTG CAGGGATTGT GCCATTGATC CAACCTGTGT CCTGTGTATG 
GATTGTTTCC AGAACAGCAT TCATAAAAAT CACAGATACA AGATGCACTC TTCAATGGGA 
GGGGGATTTT GTGACTGTGG GGATACTGAA GCCTGGAAAA CCGGCCCATA TTGTAAGATC 
CATGAACCAG GGCCATCAGA TCAGAATTCA GACTTTCGTT TGAAGGATGA ACTTCTGGCC 
CAAACCAAAA TAGTATTCCC TTTGATTATC CGGTATATAG TAGACATGTT AATCTGGGAA 
GAACAAAAGA GTTTGCCAGC ATATCTGGAT ACAGGGACAG TGGAGTGGAA CCAGTACTAC 
TGTGTACTTT ACAATGATGA ACACCACACC TATGATCACT TCATCTACAG TTTGCAGAGA 
TCGCTTGGTT GTGATCAGAA AGAAGCACAG ACATTAACCA GTGCAGCTGA GAGAGAGGGC 
CGCAAAGCTG TTAAAAAGGG TACTTTAGAG CAATGCCAGG AGGTGACAGA AAGTCTAAAG 
GCTAATTCAG AGAACGTGTC GCTGAAACCT GTCCACGTGG AGGTGCTGCA TGGTCATGTA 
ATGGCCCATC AAAGCTTTGC ACTGTGTTTA GCAATCTGGC TTAACAAACT CCTGGCCTAC 
TCTAGTGACT TCCGACAGAT CTTCTGTCAA GTGTGCCTAG AGCAAGAGCC AAAGCCTAGC 
AGTTTTAATC ACACTTGTTA TGTCAGCTTG TTAATTTTAA AGGACTCCAA GCTTCATAAA 
GGAGTGCGGA AAGTTCTGCA TGATCTGATC TTCAGCAGCT TTTTCATGGA AATGGAGTAT 
AAGAAACAAT TTGCTATAGA GTTTGTTAAG CATTACAAGC AGATACAGAA AGAATACATA 
AACGACGATC AGGACAGGAA CATTTCAGTT ACTGCAATAT CAGTTCAGGT TTTCACTGTC 
CCTACTTTGG CTCGCCTACT GATAGAAGAA CAAAATGTAA TGAAAGTCAT CACAGAGACT 
TTACTCGAGG TCATAAAAGA ATATCTTGGC TCAGACAACA AGTTCAACTT CCAGGGCTAC 
AGCCAGGAAC GCTTTTTCAT GGTATATGCC ATAATCCTTG ACCTCAAGTA TATCCTTATC 
TCTAAACCTA CATCCTGGTC TGAGAAGTTA AAAGCCAAGT TTCTGGAAGG ATTTAAATCT 
TTCCTCTATT TTTTATCCTG TATGCAGGGC ATGGAAGGGA TAAGCAGGAT GGTGGGACAG 
CACGTTGAGA TGGATTCAGA CTGGGAAGCT GTAATAGCTA TGCAGATGCA ACTGAAAAAT 
GTCTTGACGC TGATACAGGA ATGGTGTGCA ACAGATGATG ATATATTGCT CAAATCCTAC 
AAGGAATGCC ATCTGAGTCT TCTGCAGTGT TGCACCCCGG TCCCTTCCCA AGAAAAGAAG 
CTGATAAAAA TGAGTGGCCA TTCATACCAA AGCTATCCAT ACATGGTGTC CAAAGACCCA 
ATCAGCATAC ACTTACCTCT CTCTCGGATG CTTGCTGGTC TACATGCACA GATGTGTAGG 
ACTGGTGTCA TCACAAGACT GTGTGAAATT CTTCCTCCGG AAGACTTACA GGTGGAACTA 
ATGATTGAAC ACCCCTTAAG ATGCCTGGTG CTGGTGGGAC AGGTAGCAGC TGAGATGTGG 
AGAAGGAATG GCCTTACTAT TGTTAGTCAG GTTTTTTACT ATCATGATGT GAAGTGCAGG 
GAGGAGATGT ATGACAAAGA TGTTGTGATG CTACAGATTG GTGCATCCTA CCTGGATCCC 
AACAGCTTCC TACTGCTCTT GCTGCAGAGG TATGAACTGG AGGAGGCATT CTCTAATGTA 
GCTGTGCCCA CCAAAGACAT GGATGTAGCA AGGCAACACA ACATCTTAAT TGAAGAAATG 
TTACATGTCC TTATTTGTGT TATTGGTGAG CGCTATGTGC CAGGCATTAG CAATGTTACT 
AGAGAAGAGT GCACTATGAG GGAGATCATC CATCTGCTTT GTATTGAACC TATGGCTCAC 
AGTGCAATCG CCAAGGCTTT GCCAGAGACT GAAAACACTG ACATATGCCT GGATAAACTC 
ATCAGTAAAG TTGCCACTTT TAAAAAACCG GGAGTATCAG GACATGGCGT TTATGAACTA 
AAGGCTGAAT ACCTCGATCA ATTTAATGTG TTCTTTTATC ATTACACCAA AACACAACAC 
AGCAAGGCAG AACACACACA GAAGAAAAGG CGAAAACAAG AAAACAGCTC AGAAGCATTA 
GCCCCTCCTG CACCCCCCAA TTTCAGCCCA GCTTTTTGCA ACGTGACCAA GCTTCTGTGC 
TGTGATATTA TGATGCATAT TCTACGAACC ATCTTGAAGA GAGCAGCAGA GGAAGATCCA 
ATTATGTGGA CAGAAGGCAT GATACAAATG GTCCTCCATA TTATAGCCCT GGGGTTGTTA 
GAAGAGAAGC AACAGCTACA GAGGGCTGCT GAAGAAGAAG TAACATTTAA CTTCTATCAT 
AAGGCAACAA GGGTTAGGAG TACAGATGCA AATGCACAAA GTGTTGCGAC ACTGCTGGAG 
AGGCTGAAGG GCATTACCCA GCTGGATGCC CAGAAAGACA TGATCATTTG GGTGCGACAA 
GTTTTTGAAA CCGTAAAAGG GCTGAGAGAG ACATCTAGTC ACACAGTTCC CACAGATACA 
GTTTCTGACG TTGCAAAAGC TGAAGAGGGA CAAAGCCCCC AGGAAAAGGA GAAGCGCAAA 
AGAAAAGCTG AGGCGGCCCG GCTCCACCGC CAGAAGATCA TGGCACAGAT GTCAGCTTTA 
CAAAAAAATT TCATTGAAAC AAATAAGCTT CTGTATGACA CCACTGTGGA AGTACAAGAA 
AGCACTAGTC CTGTAGAAGA GAGCCTGTTA TCAGCAGATG AAGGATCGAA GGTTGCCTTG 
GGGACAAAAC GTGGCCCCAC AGTGCCTAGC AAAGATGTTC TAACATGTAT TCTATGTCAA 
GAAGAACAGG AAGTGAAATT GGACAAGCCT ACAATGGTGC TAACTGCCTG CGTCCAAAAG 
TCCACAACAC TGTCCCAAAC TCGTAGCAAA GTGGTCTCAC ACACAGGAGA AACGTTTGAT 
CCACTTTTTA CACACCCAGA GATGTTGTAT GGAACCCATA CAGGCAGCTG TGGCCATGTA 
ATGCATGTTG TGTGCTGGCA GAAGTTCTTT GAGGCAATGC AGAAGAATAC ACGGCAGCGT 
CTTCACGTGG AGCTAATTTT TGATCTTGGA AATGGCGAAT ATCTTTGTCC GCTATGCAAA 
TGCCACTGTA ACACCGTTAT ACCTATAATC CCTCATGTTG CCCAGCAAAT ACAAAGTGTG 
GATGCTGAAG CCATAAATTG TGTTTTCTCA CTTCCTCATT GGTTGGACAT AGTGGCGGCC 
AGAATATCTG GTTATAATTT AAACACCATC AAAGGAACAA AGCCAATCAC TCCTGAATTC 
TGTAAAATTT ATAAAGACCA GAATTATGAG TTCCGTTCCA TCTTGAGCTT TGGCGTGCAG 
GCTCCGCCCC GCTATCCAAC TTGCACAGAA GGAATGCTTG TTCACTTTGC CACTACAGTG 
TACCGCGTGG GCTTAAATGT TCCTCCCAAT GAGTCTGATT CACGTATGCC TGTCATGACA 
TGGAATACTT GTGCATTTAC AATCCAGTGT ATAGAAAGCT GGCTTCAGGA TGAAGAAAAA 
CCCTTGTTTG GATCCCTGCA AAACCGGCAA CTTGCTGGAT TACAAGCCTT GGTGAAATTT 
GCTGAGGCCC AAAGAGTTAC ATGCCCTCAG CCTTTGATAC GGAGACATCT TATCCGGTTA 
TTAGCAGCAT TCCTGCCAAA CATTACCCTT GAAGACACCC CATCCTTTCT GTCTTTGGAT 
GTTTTCCATC TGCTGGTGGG GTCAGTGTTG GCATTCCCAT CATTATATTG TGAAGAAAAT 
GTAGACTTAC ATCCTTCTAC CCTTGTTTCT ACCTACAACA ACCTCTACCT CTTTCACCTG 
CTCACAATGA CGCACATACT GCAAATCATT TTCTCTTTAA CTACAGATAA TGTGCCTTCT 
ACAGAACCTG ACGACTGTGA AGAAGCCCGT ACAGCTGCTA CTCTTTGTCA GGACATTTCA 
CAACTCACCA GCAACAGTTT AAAGATTGAT TTTCCGGGGT GGTATGTTTG GAAATGTGTG 
AAAGAGGGCA TCATGCCATA TCTGCGATGT GCAGCTTTGT TCTTCCATTA TTTGCTGGGA 
GTAATGCCTC CAGAGGAGCT AAAATCATCA GGCACTACAG ACAATCAGTT ATCAGCTCTT 
TGCAGCTTCC TCTGCTTGCC AACCAACATT CTTCTTCTTT TCCAAGAGAA CTGGGACATT 
GTGAAACTTC TCATTCAGAG GTTGAGTTCT GACCAATCCC TGCAGAGTGT CCTGGCTGGT 
TACACTACTG CACTCAGGTA TCCAAGGAAA CGCAATACAC TAATAGAGCT TCCAGAAGAT 
TACAGTTCCC TTCTCAACCA AGCATCCCAG TTTAGATGTC CTAAGTTTCA AGATGCTGAA 
CGGAAACACC CGGTGATGTG CCTTCTCTGT GGGGCCATTC TCTGTTCTCA GAATACATGC 
TGTCAGGAGG TGGTAAATGG AGAAGAGCTG GGAGCTTGTA CAGCACATGC CATGCACTGT 
GGAGCTGGTG TCTGCATCTT CCTAAGTATC AGAGAATGCA AAGTGGTCCT AATGGAGGGT 
AAAACCAGAG GATGTATCTA CCAGGCTCCC TATCTTGATG AATATGGAGA AACAGACCCA 
GGACTGAAAC GTGGAAACCC CTTACACCTT TCCCATGAGC GATATCGGAA ATTGCATCAG 
TTGTGGCAGC AGCACTGTAT TATTGAAGAG ATTGGACACA ACCTTGAAAT CAACCAGATG 
TTTTTTGACT TTAATTGGAG CCTGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr1 ( XM_012969237.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824691.2 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X2, mRNA.

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CloneID	OXa65688
Clone ID Related Accession (Same CDS sequence)	XM_012969237.3
Accession Version	XM_012969237.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969237.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCTG GAGGTGGAGG ATTGGAGCAA AGCAGGGAGC CAGGGGATGG AGAAATGCTT 
GCCATGAGAT TAGTCAACGC TTTGGATTTT GCCTCTGCCT TTCACCAACA CGTGGCAAAG 
CATGTCCCCC AGATTTATAC AGATGAGCTG CAGCCACAAG TGGAGAAGCA AGAGGAGCTG 
GCACAGAAAC TTATTTTAAC CCCACTGGAG TGGTGCCTCT TTAGAGAAGA TCCAAATGTA 
TTCAAGGAGA AGTTACGGCA CAGTGCAGCT CCTCCATTAT GTGGAAGAGT CTTTAGAGGA 
GGGGAGACAA CCTATTCCTG CAGGGATTGT GCCATTGATC CAACCTGTGT CCTGTGTATG 
GATTGTTTCC AGAACAGCAT TCATAAAAAT CACAGATACA AGATGCACTC TTCAATGGGA 
GGGGGATTTT GTGACTGTGG GGATACTGAA GCCTGGAAAA CCGGCCCATA TTGTAAGATC 
CATGAACCAG GGCCATCAGA TCAGAATTCA GACTTTCGTT TGAAGGATGA ACTTCTGGCC 
CAAACCAAAA TAGTATTCCC TTTGATTATC CGGTATATAG TAGACATGTT AATCTGGGAA 
GAACAAAAGA GTTTGCCAGC ATATCTGGAT ACAGGGACAG TGGAGTGGAA CCAGTACTAC 
TGTGTACTTT ACAATGATGA ACACCACACC TATGATCACT TCATCTACAG TTTGCAGAGA 
TCGCTTGGTT GTGATCAGAA AGAAGCACAG ACATTAACCA GTGCAGCTGA GAGAGAGGGC 
CGCAAAGCTG TTAAAAAGGG TACTTTAGAG CAATGCCAGG AGGTGACAGA AAGTCTAAAG 
GCTAATTCAG AGAACGTGTC GCTGAAACCT GTCCACGTGG AGGTGCTGCA TGGTCATGTA 
ATGGCCCATC AAAGCTTTGC ACTGTGTTTA GCAATCTGGC TTAACAAACT CCTGGCCTAC 
TCTAGTGACT TCCGACAGAT CTTCTGTCAA GTGTGCCTAG AGCAAGAGCC AAAGCCTAGC 
AGTTTTAATC ACACTTGTTA TGTCAGCTTG TTAATTTTAA AGGACTCCAA GCTTCATAAA 
GGAGTGCGGA AAGTTCTGCA TGATCTGATC TTCAGCAGCT TTTTCATGGA AATGGAGTAT 
AAGAAACAAT TTGCTATAGA GTTTGTTAAG CATTACAAGC AGATACAGAA AGAATACATA 
AACGACGATC AGGACAGGAA CATTTCAGTT ACTGCAATAT CAGTTCAGGT TTTCACTGTC 
CCTACTTTGG CTCGCCTACT GATAGAAGAA CAAAATGTAA TGAAAGTCAT CACAGAGACT 
TTACTCGAGG TCATAAAAGA ATATCTTGGC TCAGACAACA AGTTCAACTT CCAGGGCTAC 
AGCCAGGAAC GCTTTTTCAT GGTATATGCC ATAATCCTTG ACCTCAAGTA TATCCTTATC 
TCTAAACCTA CATCCTGGTC TGAGAAGTTA AAAGCCAAGT TTCTGGAAGG ATTTAAATCT 
TTCCTCTATT TTTTATCCTG TATGCAGGGC ATGGAAGGGA TAAGCAGGAT GGTGGGACAG 
CACGTTGAGA TGGATTCAGA CTGGGAAGCT GTAATAGCTA TGCAGATGCA ACTGAAAAAT 
GTCTTGACGC TGATACAGGA ATGGTGTGCA ACAGATGATG ATATATTGCT CAAATCCTAC 
AAGGAATGCC ATCTGAGTCT TCTGCAGTGT TGCACCCCGG TCCCTTCCCA AGAAAAGAAG 
CTGATAAAAA TGAGTGGCCA TTCATACCAA AGCTATCCAT ACATGGTGTC CAAAGACCCA 
ATCAGCATAC ACTTACCTCT CTCTCGGATG CTTGCTGGTC TACATGCACA GATGTGTAGG 
ACTGGTGTCA TCACAAGACT GTGTGAAATT CTTCCTCCGG AAGACTTACA GGTGGAACTA 
ATGATTGAAC ACCCCTTAAG ATGCCTGGTG CTGGTGGGAC AGGTAGCAGC TGAGATGTGG 
AGAAGGAATG GCCTTACTAT TGTTAGTCAG GTTTTTTACT ATCATGATGT GAAGTGCAGG 
GAGGAGATGT ATGACAAAGA TGTTGTGATG CTACAGATTG GTGCATCCTA CCTGGATCCC 
AACAGCTTCC TACTGCTCTT GCTGCAGAGG TATGAACTGG AGGAGGCATT CTCTAATGTA 
GCTGTGCCCA CCAAAGACAT GGATGTAGCA AGGCAACACA ACATCTTAAT TGAAGAAATG 
TTACATGTCC TTATTTGTGT TATTGGTGAG CGCTATGTGC CAGGCATTAG CAATGTTACT 
AGAGAAGAGT GCACTATGAG GGAGATCATC CATCTGCTTT GTATTGAACC TATGGCTCAC 
AGTGCAATCG CCAAGGCTTT GCCAGAGACT GAAAACACTG ACATATGCCT GGATAAACTC 
ATCAGTAAAG TTGCCACTTT TAAAAAACCG GGAGTATCAG GACATGGCGT TTATGAACTA 
AAGGCTGAAT ACCTCGATCA ATTTAATGTG TTCTTTTATC ATTACACCAA AACACAACAC 
AGCAAGGCAG AACACACACA GAAGAAAAGG CGAAAACAAG AAAACAGCTC AGAAGCATTA 
GCCCCTCCTG CACCCCCCAA TTTCAGCCCA GCTTTTTGCA ACGTGACCAA GCTTCTGTGC 
TGTGATATTA TGATGCATAT TCTACGAACC ATCTTGAAGA GAGCAGCAGA GGAAGATCCA 
ATTATGTGGA CAGAAGGCAT GATACAAATG GTCCTCCATA TTATAGCCCT GGGGTTGTTA 
GAAGAGAAGC AACAGCTACA GAGGGCTGCT GAAGAAGAAG TAACATTTAA CTTCTATCAT 
AAGGCAACAA GGGTTAGGAG TACAGATGCA AATGCACAAA GTGTTGCGAC ACTGCTGGAG 
AGGCTGAAGG GCATTACCCA GCTGGATGCC CAGAAAGACA TGATCATTTG GGTGCGACAA 
GTTTTTGAAA CCGTAAAAGG GCTGAGAGAG ACATCTAGTC ACACAGTTCC CACAGATACA 
GTTTCTGACG TTGCAAAAGC TGAAGAGGGA CAAAGCCCCC AGGAAAAGGA GAAGCGCAAA 
AGAAAAGCTG AGGCGGCCCG GCTCCACCGC CAGAAGATCA TGGCACAGAT GTCAGCTTTA 
CAAAAAAATT TCATTGAAAC AAATAAGCTT CTGTATGACA CCACTGTGGA AGTACAAGAA 
AGCACTAGTC CTGTAGAAGA GAGCCTGTTA TCAGCAGATG AAGGATCGAA GGTTGCCTTG 
GGGACAAAAC GTGGCCCCAC AGTGCCTAGC AAAGATGTTC TAACATGTAT TCTATGTCAA 
GAAGAACAGG AAGTGAAATT GGACAAGCCT ACAATGGTGC TAACTGCCTG CGTCCAAAAG 
TCCACAACAC TGTCCCAAAC TCGTAGCAAA GTGGTCTCAC ACACAGGAGA AACGTTTGAT 
CCACTTTTTA CACACCCAGA GATGTTGTAT GGAACCCATA CAGGCAGCTG TGGCCATGTA 
ATGCATGTTG TGTGCTGGCA GAAGTTCTTT GAGGCAATGC AGAAGAATAC ACGGCAGCGT 
CTTCACGTGG AGCTAATTTT TGATCTTGGA AATGGCGAAT ATCTTTGTCC GCTATGCAAA 
TGCCACTGTA ACACCGTTAT ACCTATAATC CCTCATGTTG CCCAGCAAAT ACAAAGTGTG 
GATGCTGAAG CCATAAATTG TGTTTTCTCA CTTCCTCATT GGTTGGACAT AGTGGCGGCC 
AGAATATCTG GTTATAATTT AAACACCATC AAAGGAACAA AGCCAATCAC TCCTGAATTC 
TGTAAAATTT ATAAAGACCA GAATTATGAG TTCCGTTCCA TCTTGAGCTT TGGCGTGCAG 
GCTCCGCCCC GCTATCCAAC TTGCACAGAA GGAATGCTTG TTCACTTTGC CACTACAGTG 
TACCGCGTGG GCTTAAATGT TCCTCCCAAT GAGTCTGATT CACGTATGCC TGTCATGACA 
TGGAATACTT GTGCATTTAC AATCCAGTGT ATAGAAAGCT GGCTTCAGGA TGAAGAAAAA 
CCCTTGTTTG GATCCCTGCA AAACCGGCAA CTTGCTGGAT TACAAGCCTT GGTGAAATTT 
GCTGAGGCCC AAAGAGTTAC ATGCCCTCAG CCTTTGATAC GGAGACATCT TATCCGGTTA 
TTAGCAGCAT TCCTGCCAAA CATTACCCTT GAAGACACCC CATCCTTTCT GTCTTTGGAT 
GTTTTCCATC TGCTGGTGGG GTCAGTGTTG GCATTCCCAT CATTATATTG TGAAGAAAAT 
GTAGACTTAC ATCCTTCTAC CCTTGTTTCT ACCTACAACA ACCTCTACCT CTTTCACCTG 
CTCACAATGA CGCACATACT GCAAATCATT TTCTCTTTAA CTACAGATAA TGTGCCTTCT 
ACAGAACCTG ACGACTGTGA AGAAGCCCGT ACAGCTGCTA CTCTTTGTCA GGACATTTCA 
CAACTCACCA GCAATTTAAA GATTGATTTT CCGGGGTGGT ATGTTTGGAA ATGTGTGAAA 
GAGGGCATCA TGCCATATCT GCGATGTGCA GCTTTGTTCT TCCATTATTT GCTGGGAGTA 
ATGCCTCCAG AGGAGCTAAA ATCATCAGGC ACTACAGACA ATCAGTTATC AGCTCTTTGC 
AGCTTCCTCT GCTTGCCAAC CAACATTCTT CTTCTTTTCC AAGAGAACTG GGACATTGTG 
AAACTTCTCA TTCAGAGGTT GAGTTCTGAC CAATCCCTGC AGAGTGTCCT GGCTGGTTAC 
ACTACTGCAC TCAGGTATCC AAGGAAACGC AATACACTAA TAGAGCTTCC AGAAGATTAC 
AGTTCCCTTC TCAACCAAGC ATCCCAGTTT AGATGTCCTA AGTTTCAAGA TGCTGAACGG 
AAACACCCGG TGATGTGCCT TCTCTGTGGG GCCATTCTCT GTTCTCAGAA TACATGCTGT 
CAGGAGGTGG TAAATGGAGA AGAGCTGGGA GCTTGTACAG CACATGCCAT GCACTGTGGA 
GCTGGTGTCT GCATCTTCCT AAGTATCAGA GAATGCAAAG TGGTCCTAAT GGAGGGTAAA 
ACCAGAGGAT GTATCTACCA GGCTCCCTAT CTTGATGAAT ATGGAGAAAC AGACCCAGGA 
CTGAAACGTG GAAACCCCTT ACACCTTTCC CATGAGCGAT ATCGGAAATT GCATCAGTTG 
TGGCAGCAGC ACTGTATTAT TGAAGAGATT GGACACAACC TTGAAATCAA CCAGATGTTT 
TTTGACTTTA ATTGGAGCCT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824691.2
CDS	484..5730
Translation	MESGGGGLEQSREPGDGEMLAMRLVNALDFASAFHQHVAKHVPQIYTDELQPQVEKQEELAQKLILTPLEWCLFREDPNVFKEKLRHSAAPPLCGRVFRGGETTYSCRDCAIDPTCVLCMDCFQNSIHKNHRYKMHSSMGGGFCDCGDTEAWKTGPYCKIHEPGPSDQNSDFRLKDELLAQTKIVFPLIIRYIVDMLIWEEQKSLPAYLDTGTVEWNQYYCVLYNDEHHTYDHFIYSLQRSLGCDQKEAQTLTSAAEREGRKAVKKGTLEQCQEVTESLKANSENVSLKPVHVEVLHGHVMAHQSFALCLAIWLNKLLAYSSDFRQIFCQVCLEQEPKPSSFNHTCYVSLLILKDSKLHKGVRKVLHDLIFSSFFMEMEYKKQFAIEFVKHYKQIQKEYINDDQDRNISVTAISVQVFTVPTLARLLIEEQNVMKVITETLLEVIKEYLGSDNKFNFQGYSQERFFMVYAIILDLKYILISKPTSWSEKLKAKFLEGFKSFLYFLSCMQGMEGISRMVGQHVEMDSDWEAVIAMQMQLKNVLTLIQEWCATDDDILLKSYKECHLSLLQCCTPVPSQEKKLIKMSGHSYQSYPYMVSKDPISIHLPLSRMLAGLHAQMCRTGVITRLCEILPPEDLQVELMIEHPLRCLVLVGQVAAEMWRRNGLTIVSQVFYYHDVKCREEMYDKDVVMLQIGASYLDPNSFLLLLLQRYELEEAFSNVAVPTKDMDVARQHNILIEEMLHVLICVIGERYVPGISNVTREECTMREIIHLLCIEPMAHSAIAKALPETENTDICLDKLISKVATFKKPGVSGHGVYELKAEYLDQFNVFFYHYTKTQHSKAEHTQKKRRKQENSSEALAPPAPPNFSPAFCNVTKLLCCDIMMHILRTILKRAAEEDPIMWTEGMIQMVLHIIALGLLEEKQQLQRAAEEEVTFNFYHKATRVRSTDANAQSVATLLERLKGITQLDAQKDMIIWVRQVFETVKGLRETSSHTVPTDTVSDVAKAEEGQSPQEKEKRKRKAEAARLHRQKIMAQMSALQKNFIETNKLLYDTTVEVQESTSPVEESLLSADEGSKVALGTKRGPTVPSKDVLTCILCQEEQEVKLDKPTMVLTACVQKSTTLSQTRSKVVSHTGETFDPLFTHPEMLYGTHTGSCGHVMHVVCWQKFFEAMQKNTRQRLHVELIFDLGNGEYLCPLCKCHCNTVIPIIPHVAQQIQSVDAEAINCVFSLPHWLDIVAARISGYNLNTIKGTKPITPEFCKIYKDQNYEFRSILSFGVQAPPRYPTCTEGMLVHFATTVYRVGLNVPPNESDSRMPVMTWNTCAFTIQCIESWLQDEEKPLFGSLQNRQLAGLQALVKFAEAQRVTCPQPLIRRHLIRLLAAFLPNITLEDTPSFLSLDVFHLLVGSVLAFPSLYCEENVDLHPSTLVSTYNNLYLFHLLTMTHILQIIFSLTTDNVPSTEPDDCEEARTAATLCQDISQLTSNLKIDFPGWYVWKCVKEGIMPYLRCAALFFHYLLGVMPPEELKSSGTTDNQLSALCSFLCLPTNILLLFQENWDIVKLLIQRLSSDQSLQSVLAGYTTALRYPRKRNTLIELPEDYSSLLNQASQFRCPKFQDAERKHPVMCLLCGAILCSQNTCCQEVVNGEELGACTAHAMHCGAGVCIFLSIRECKVVLMEGKTRGCIYQAPYLDEYGETDPGLKRGNPLHLSHERYRKLHQLWQQHCIIEEIGHNLEINQMFFDFNWSLL

Target ORF information:

RefSeq Version	XM_012969237.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969237.3

ORF Insert Sequence:

ATGGAGTCTG GAGGTGGAGG ATTGGAGCAA AGCAGGGAGC CAGGGGATGG AGAAATGCTT 
GCCATGAGAT TAGTCAACGC TTTGGATTTT GCCTCTGCCT TTCACCAACA CGTGGCAAAG 
CATGTCCCCC AGATTTATAC AGATGAGCTG CAGCCACAAG TGGAGAAGCA AGAGGAGCTG 
GCACAGAAAC TTATTTTAAC CCCACTGGAG TGGTGCCTCT TTAGAGAAGA TCCAAATGTA 
TTCAAGGAGA AGTTACGGCA CAGTGCAGCT CCTCCATTAT GTGGAAGAGT CTTTAGAGGA 
GGGGAGACAA CCTATTCCTG CAGGGATTGT GCCATTGATC CAACCTGTGT CCTGTGTATG 
GATTGTTTCC AGAACAGCAT TCATAAAAAT CACAGATACA AGATGCACTC TTCAATGGGA 
GGGGGATTTT GTGACTGTGG GGATACTGAA GCCTGGAAAA CCGGCCCATA TTGTAAGATC 
CATGAACCAG GGCCATCAGA TCAGAATTCA GACTTTCGTT TGAAGGATGA ACTTCTGGCC 
CAAACCAAAA TAGTATTCCC TTTGATTATC CGGTATATAG TAGACATGTT AATCTGGGAA 
GAACAAAAGA GTTTGCCAGC ATATCTGGAT ACAGGGACAG TGGAGTGGAA CCAGTACTAC 
TGTGTACTTT ACAATGATGA ACACCACACC TATGATCACT TCATCTACAG TTTGCAGAGA 
TCGCTTGGTT GTGATCAGAA AGAAGCACAG ACATTAACCA GTGCAGCTGA GAGAGAGGGC 
CGCAAAGCTG TTAAAAAGGG TACTTTAGAG CAATGCCAGG AGGTGACAGA AAGTCTAAAG 
GCTAATTCAG AGAACGTGTC GCTGAAACCT GTCCACGTGG AGGTGCTGCA TGGTCATGTA 
ATGGCCCATC AAAGCTTTGC ACTGTGTTTA GCAATCTGGC TTAACAAACT CCTGGCCTAC 
TCTAGTGACT TCCGACAGAT CTTCTGTCAA GTGTGCCTAG AGCAAGAGCC AAAGCCTAGC 
AGTTTTAATC ACACTTGTTA TGTCAGCTTG TTAATTTTAA AGGACTCCAA GCTTCATAAA 
GGAGTGCGGA AAGTTCTGCA TGATCTGATC TTCAGCAGCT TTTTCATGGA AATGGAGTAT 
AAGAAACAAT TTGCTATAGA GTTTGTTAAG CATTACAAGC AGATACAGAA AGAATACATA 
AACGACGATC AGGACAGGAA CATTTCAGTT ACTGCAATAT CAGTTCAGGT TTTCACTGTC 
CCTACTTTGG CTCGCCTACT GATAGAAGAA CAAAATGTAA TGAAAGTCAT CACAGAGACT 
TTACTCGAGG TCATAAAAGA ATATCTTGGC TCAGACAACA AGTTCAACTT CCAGGGCTAC 
AGCCAGGAAC GCTTTTTCAT GGTATATGCC ATAATCCTTG ACCTCAAGTA TATCCTTATC 
TCTAAACCTA CATCCTGGTC TGAGAAGTTA AAAGCCAAGT TTCTGGAAGG ATTTAAATCT 
TTCCTCTATT TTTTATCCTG TATGCAGGGC ATGGAAGGGA TAAGCAGGAT GGTGGGACAG 
CACGTTGAGA TGGATTCAGA CTGGGAAGCT GTAATAGCTA TGCAGATGCA ACTGAAAAAT 
GTCTTGACGC TGATACAGGA ATGGTGTGCA ACAGATGATG ATATATTGCT CAAATCCTAC 
AAGGAATGCC ATCTGAGTCT TCTGCAGTGT TGCACCCCGG TCCCTTCCCA AGAAAAGAAG 
CTGATAAAAA TGAGTGGCCA TTCATACCAA AGCTATCCAT ACATGGTGTC CAAAGACCCA 
ATCAGCATAC ACTTACCTCT CTCTCGGATG CTTGCTGGTC TACATGCACA GATGTGTAGG 
ACTGGTGTCA TCACAAGACT GTGTGAAATT CTTCCTCCGG AAGACTTACA GGTGGAACTA 
ATGATTGAAC ACCCCTTAAG ATGCCTGGTG CTGGTGGGAC AGGTAGCAGC TGAGATGTGG 
AGAAGGAATG GCCTTACTAT TGTTAGTCAG GTTTTTTACT ATCATGATGT GAAGTGCAGG 
GAGGAGATGT ATGACAAAGA TGTTGTGATG CTACAGATTG GTGCATCCTA CCTGGATCCC 
AACAGCTTCC TACTGCTCTT GCTGCAGAGG TATGAACTGG AGGAGGCATT CTCTAATGTA 
GCTGTGCCCA CCAAAGACAT GGATGTAGCA AGGCAACACA ACATCTTAAT TGAAGAAATG 
TTACATGTCC TTATTTGTGT TATTGGTGAG CGCTATGTGC CAGGCATTAG CAATGTTACT 
AGAGAAGAGT GCACTATGAG GGAGATCATC CATCTGCTTT GTATTGAACC TATGGCTCAC 
AGTGCAATCG CCAAGGCTTT GCCAGAGACT GAAAACACTG ACATATGCCT GGATAAACTC 
ATCAGTAAAG TTGCCACTTT TAAAAAACCG GGAGTATCAG GACATGGCGT TTATGAACTA 
AAGGCTGAAT ACCTCGATCA ATTTAATGTG TTCTTTTATC ATTACACCAA AACACAACAC 
AGCAAGGCAG AACACACACA GAAGAAAAGG CGAAAACAAG AAAACAGCTC AGAAGCATTA 
GCCCCTCCTG CACCCCCCAA TTTCAGCCCA GCTTTTTGCA ACGTGACCAA GCTTCTGTGC 
TGTGATATTA TGATGCATAT TCTACGAACC ATCTTGAAGA GAGCAGCAGA GGAAGATCCA 
ATTATGTGGA CAGAAGGCAT GATACAAATG GTCCTCCATA TTATAGCCCT GGGGTTGTTA 
GAAGAGAAGC AACAGCTACA GAGGGCTGCT GAAGAAGAAG TAACATTTAA CTTCTATCAT 
AAGGCAACAA GGGTTAGGAG TACAGATGCA AATGCACAAA GTGTTGCGAC ACTGCTGGAG 
AGGCTGAAGG GCATTACCCA GCTGGATGCC CAGAAAGACA TGATCATTTG GGTGCGACAA 
GTTTTTGAAA CCGTAAAAGG GCTGAGAGAG ACATCTAGTC ACACAGTTCC CACAGATACA 
GTTTCTGACG TTGCAAAAGC TGAAGAGGGA CAAAGCCCCC AGGAAAAGGA GAAGCGCAAA 
AGAAAAGCTG AGGCGGCCCG GCTCCACCGC CAGAAGATCA TGGCACAGAT GTCAGCTTTA 
CAAAAAAATT TCATTGAAAC AAATAAGCTT CTGTATGACA CCACTGTGGA AGTACAAGAA 
AGCACTAGTC CTGTAGAAGA GAGCCTGTTA TCAGCAGATG AAGGATCGAA GGTTGCCTTG 
GGGACAAAAC GTGGCCCCAC AGTGCCTAGC AAAGATGTTC TAACATGTAT TCTATGTCAA 
GAAGAACAGG AAGTGAAATT GGACAAGCCT ACAATGGTGC TAACTGCCTG CGTCCAAAAG 
TCCACAACAC TGTCCCAAAC TCGTAGCAAA GTGGTCTCAC ACACAGGAGA AACGTTTGAT 
CCACTTTTTA CACACCCAGA GATGTTGTAT GGAACCCATA CAGGCAGCTG TGGCCATGTA 
ATGCATGTTG TGTGCTGGCA GAAGTTCTTT GAGGCAATGC AGAAGAATAC ACGGCAGCGT 
CTTCACGTGG AGCTAATTTT TGATCTTGGA AATGGCGAAT ATCTTTGTCC GCTATGCAAA 
TGCCACTGTA ACACCGTTAT ACCTATAATC CCTCATGTTG CCCAGCAAAT ACAAAGTGTG 
GATGCTGAAG CCATAAATTG TGTTTTCTCA CTTCCTCATT GGTTGGACAT AGTGGCGGCC 
AGAATATCTG GTTATAATTT AAACACCATC AAAGGAACAA AGCCAATCAC TCCTGAATTC 
TGTAAAATTT ATAAAGACCA GAATTATGAG TTCCGTTCCA TCTTGAGCTT TGGCGTGCAG 
GCTCCGCCCC GCTATCCAAC TTGCACAGAA GGAATGCTTG TTCACTTTGC CACTACAGTG 
TACCGCGTGG GCTTAAATGT TCCTCCCAAT GAGTCTGATT CACGTATGCC TGTCATGACA 
TGGAATACTT GTGCATTTAC AATCCAGTGT ATAGAAAGCT GGCTTCAGGA TGAAGAAAAA 
CCCTTGTTTG GATCCCTGCA AAACCGGCAA CTTGCTGGAT TACAAGCCTT GGTGAAATTT 
GCTGAGGCCC AAAGAGTTAC ATGCCCTCAG CCTTTGATAC GGAGACATCT TATCCGGTTA 
TTAGCAGCAT TCCTGCCAAA CATTACCCTT GAAGACACCC CATCCTTTCT GTCTTTGGAT 
GTTTTCCATC TGCTGGTGGG GTCAGTGTTG GCATTCCCAT CATTATATTG TGAAGAAAAT 
GTAGACTTAC ATCCTTCTAC CCTTGTTTCT ACCTACAACA ACCTCTACCT CTTTCACCTG 
CTCACAATGA CGCACATACT GCAAATCATT TTCTCTTTAA CTACAGATAA TGTGCCTTCT 
ACAGAACCTG ACGACTGTGA AGAAGCCCGT ACAGCTGCTA CTCTTTGTCA GGACATTTCA 
CAACTCACCA GCAATTTAAA GATTGATTTT CCGGGGTGGT ATGTTTGGAA ATGTGTGAAA 
GAGGGCATCA TGCCATATCT GCGATGTGCA GCTTTGTTCT TCCATTATTT GCTGGGAGTA 
ATGCCTCCAG AGGAGCTAAA ATCATCAGGC ACTACAGACA ATCAGTTATC AGCTCTTTGC 
AGCTTCCTCT GCTTGCCAAC CAACATTCTT CTTCTTTTCC AAGAGAACTG GGACATTGTG 
AAACTTCTCA TTCAGAGGTT GAGTTCTGAC CAATCCCTGC AGAGTGTCCT GGCTGGTTAC 
ACTACTGCAC TCAGGTATCC AAGGAAACGC AATACACTAA TAGAGCTTCC AGAAGATTAC 
AGTTCCCTTC TCAACCAAGC ATCCCAGTTT AGATGTCCTA AGTTTCAAGA TGCTGAACGG 
AAACACCCGG TGATGTGCCT TCTCTGTGGG GCCATTCTCT GTTCTCAGAA TACATGCTGT 
CAGGAGGTGG TAAATGGAGA AGAGCTGGGA GCTTGTACAG CACATGCCAT GCACTGTGGA 
GCTGGTGTCT GCATCTTCCT AAGTATCAGA GAATGCAAAG TGGTCCTAAT GGAGGGTAAA 
ACCAGAGGAT GTATCTACCA GGCTCCCTAT CTTGATGAAT ATGGAGAAAC AGACCCAGGA 
CTGAAACGTG GAAACCCCTT ACACCTTTCC CATGAGCGAT ATCGGAAATT GCATCAGTTG 
TGGCAGCAGC ACTGTATTAT TGAAGAGATT GGACACAACC TTGAAATCAA CCAGATGTTT 
TTTGACTTTA ATTGGAGCCT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr1 ( XM_012969238.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824692.2 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa65689
Clone ID Related Accession (Same CDS sequence)	XM_012969238.3
Accession Version	XM_012969238.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012969238.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTT TCCAGAACAG CATTCATAAA AATCACAGAT ACAAGATGCA CTCTTCAATG 
GGAGGGGGAT TTTGTGACTG TGGGGATACT GAAGCCTGGA AAACCGGCCC ATATTGTAAG 
ATCCATGAAC CAGGGCCATC AGATCAGAAT TCAGACTTTC GTTTGAAGGA TGAACTTCTG 
GCCCAAACCA AAATAGTATT CCCTTTGATT ATCCGGTATA TAGTAGACAT GTTAATCTGG 
GAAGAACAAA AGAGTTTGCC AGCATATCTG GATACAGGGA CAGTGGAGTG GAACCAGTAC 
TACTGTGTAC TTTACAATGA TGAACACCAC ACCTATGATC ACTTCATCTA CAGTTTGCAG 
AGATCGCTTG GTTGTGATCA GAAAGAAGCA CAGACATTAA CCAGTGCAGC TGAGAGAGAG 
GGCCGCAAAG CTGTTAAAAA GGGTACTTTA GAGCAATGCC AGGAGGTGAC AGAAAGTCTA 
AAGGCTAATT CAGAGAACGT GTCGCTGAAA CCTGTCCACG TGGAGGTGCT GCATGGTCAT 
GTAATGGCCC ATCAAAGCTT TGCACTGTGT TTAGCAATCT GGCTTAACAA ACTCCTGGCC 
TACTCTAGTG ACTTCCGACA GATCTTCTGT CAAGTGTGCC TAGAGCAAGA GCCAAAGCCT 
AGCAGTTTTA ATCACACTTG TTATGTCAGC TTGTTAATTT TAAAGGACTC CAAGCTTCAT 
AAAGGAGTGC GGAAAGTTCT GCATGATCTG ATCTTCAGCA GCTTTTTCAT GGAAATGGAG 
TATAAGAAAC AATTTGCTAT AGAGTTTGTT AAGCATTACA AGCAGATACA GAAAGAATAC 
ATAAACGACG ATCAGGACAG GAACATTTCA GTTACTGCAA TATCAGTTCA GGTTTTCACT 
GTCCCTACTT TGGCTCGCCT ACTGATAGAA GAACAAAATG TAATGAAAGT CATCACAGAG 
ACTTTACTCG AGGTCATAAA AGAATATCTT GGCTCAGACA ACAAGTTCAA CTTCCAGGGC 
TACAGCCAGG AACGCTTTTT CATGGTATAT GCCATAATCC TTGACCTCAA GTATATCCTT 
ATCTCTAAAC CTACATCCTG GTCTGAGAAG TTAAAAGCCA AGTTTCTGGA AGGATTTAAA 
TCTTTCCTCT ATTTTTTATC CTGTATGCAG GGCATGGAAG GGATAAGCAG GATGGTGGGA 
CAGCACGTTG AGATGGATTC AGACTGGGAA GCTGTAATAG CTATGCAGAT GCAACTGAAA 
AATGTCTTGA CGCTGATACA GGAATGGTGT GCAACAGATG ATGATATATT GCTCAAATCC 
TACAAGGAAT GCCATCTGAG TCTTCTGCAG TGTTGCACCC CGGTCCCTTC CCAAGAAAAG 
AAGCTGATAA AAATGAGTGG CCATTCATAC CAAAGCTATC CATACATGGT GTCCAAAGAC 
CCAATCAGCA TACACTTACC TCTCTCTCGG ATGCTTGCTG GTCTACATGC ACAGATGTGT 
AGGACTGGTG TCATCACAAG ACTGTGTGAA ATTCTTCCTC CGGAAGACTT ACAGGTGGAA 
CTAATGATTG AACACCCCTT AAGATGCCTG GTGCTGGTGG GACAGGTAGC AGCTGAGATG 
TGGAGAAGGA ATGGCCTTAC TATTGTTAGT CAGGTTTTTT ACTATCATGA TGTGAAGTGC 
AGGGAGGAGA TGTATGACAA AGATGTTGTG ATGCTACAGA TTGGTGCATC CTACCTGGAT 
CCCAACAGCT TCCTACTGCT CTTGCTGCAG AGGTATGAAC TGGAGGAGGC ATTCTCTAAT 
GTAGCTGTGC CCACCAAAGA CATGGATGTA GCAAGGCAAC ACAACATCTT AATTGAAGAA 
ATGTTACATG TCCTTATTTG TGTTATTGGT GAGCGCTATG TGCCAGGCAT TAGCAATGTT 
ACTAGAGAAG AGTGCACTAT GAGGGAGATC ATCCATCTGC TTTGTATTGA ACCTATGGCT 
CACAGTGCAA TCGCCAAGGC TTTGCCAGAG ACTGAAAACA CTGACATATG CCTGGATAAA 
CTCATCAGTA AAGTTGCCAC TTTTAAAAAA CCGGGAGTAT CAGGACATGG CGTTTATGAA 
CTAAAGGCTG AATACCTCGA TCAATTTAAT GTGTTCTTTT ATCATTACAC CAAAACACAA 
CACAGCAAGG CAGAACACAC ACAGAAGAAA AGGCGAAAAC AAGAAAACAG CTCAGAAGCA 
TTAGCCCCTC CTGCACCCCC CAATTTCAGC CCAGCTTTTT GCAACGTGAC CAAGCTTCTG 
TGCTGTGATA TTATGATGCA TATTCTACGA ACCATCTTGA AGAGAGCAGC AGAGGAAGAT 
CCAATTATGT GGACAGAAGG CATGATACAA ATGGTCCTCC ATATTATAGC CCTGGGGTTG 
TTAGAAGAGA AGCAACAGCT ACAGAGGGCT GCTGAAGAAG AAGTAACATT TAACTTCTAT 
CATAAGGCAA CAAGGGTTAG GAGTACAGAT GCAAATGCAC AAAGTGTTGC GACACTGCTG 
GAGAGGCTGA AGGGCATTAC CCAGCTGGAT GCCCAGAAAG ACATGATCAT TTGGGTGCGA 
CAAGTTTTTG AAACCGTAAA AGGGCTGAGA GAGACATCTA GTCACACAGT TCCCACAGAT 
ACAGTTTCTG ACGTTGCAAA AGCTGAAGAG GGACAAAGCC CCCAGGAAAA GGAGAAGCGC 
AAAAGAAAAG CTGAGGCGGC CCGGCTCCAC CGCCAGAAGA TCATGGCACA GATGTCAGCT 
TTACAAAAAA ATTTCATTGA AACAAATAAG CTTCTGTATG ACACCACTGT GGAAGTACAA 
GAAAGCACTA GTCCTGTAGA AGAGAGCCTG TTATCAGCAG ATGAAGGATC GAAGGTTGCC 
TTGGGGACAA AACGTGGCCC CACAGTGCCT AGCAAAGATG TTCTAACATG TATTCTATGT 
CAAGAAGAAC AGGAAGTGAA ATTGGACAAG CCTACAATGG TGCTAACTGC CTGCGTCCAA 
AAGTCCACAA CACTGTCCCA AACTCGTAGC AAAGTGGTCT CACACACAGG AGAAACGTTT 
GATCCACTTT TTACACACCC AGAGATGTTG TATGGAACCC ATACAGGCAG CTGTGGCCAT 
GTAATGCATG TTGTGTGCTG GCAGAAGTTC TTTGAGGCAA TGCAGAAGAA TACACGGCAG 
CGTCTTCACG TGGAGCTAAT TTTTGATCTT GGAAATGGCG AATATCTTTG TCCGCTATGC 
AAATGCCACT GTAACACCGT TATACCTATA ATCCCTCATG TTGCCCAGCA AATACAAAGT 
GTGGATGCTG AAGCCATAAA TTGTGTTTTC TCACTTCCTC ATTGGTTGGA CATAGTGGCG 
GCCAGAATAT CTGGTTATAA TTTAAACACC ATCAAAGGAA CAAAGCCAAT CACTCCTGAA 
TTCTGTAAAA TTTATAAAGA CCAGAATTAT GAGTTCCGTT CCATCTTGAG CTTTGGCGTG 
CAGGCTCCGC CCCGCTATCC AACTTGCACA GAAGGAATGC TTGTTCACTT TGCCACTACA 
GTGTACCGCG TGGGCTTAAA TGTTCCTCCC AATGAGTCTG ATTCACGTAT GCCTGTCATG 
ACATGGAATA CTTGTGCATT TACAATCCAG TGTATAGAAA GCTGGCTTCA GGATGAAGAA 
AAACCCTTGT TTGGATCCCT GCAAAACCGG CAACTTGCTG GATTACAAGC CTTGGTGAAA 
TTTGCTGAGG CCCAAAGAGT TACATGCCCT CAGCCTTTGA TACGGAGACA TCTTATCCGG 
TTATTAGCAG CATTCCTGCC AAACATTACC CTTGAAGACA CCCCATCCTT TCTGTCTTTG 
GATGTTTTCC ATCTGCTGGT GGGGTCAGTG TTGGCATTCC CATCATTATA TTGTGAAGAA 
AATGTAGACT TACATCCTTC TACCCTTGTT TCTACCTACA ACAACCTCTA CCTCTTTCAC 
CTGCTCACAA TGACGCACAT ACTGCAAATC ATTTTCTCTT TAACTACAGA TAATGTGCCT 
TCTACAGAAC CTGACGACTG TGAAGAAGCC CGTACAGCTG CTACTCTTTG TCAGGACATT 
TCACAACTCA CCAGCAACAG TTTAAAGATT GATTTTCCGG GGTGGTATGT TTGGAAATGT 
GTGAAAGAGG GCATCATGCC ATATCTGCGA TGTGCAGCTT TGTTCTTCCA TTATTTGCTG 
GGAGTAATGC CTCCAGAGGA GCTAAAATCA TCAGGCACTA CAGACAATCA GTTATCAGCT 
CTTTGCAGCT TCCTCTGCTT GCCAACCAAC ATTCTTCTTC TTTTCCAAGA GAACTGGGAC 
ATTGTGAAAC TTCTCATTCA GAGGTTGAGT TCTGACCAAT CCCTGCAGAG TGTCCTGGCT 
GGTTACACTA CTGCACTCAG GTATCCAAGG AAACGCAATA CACTAATAGA GCTTCCAGAA 
GATTACAGTT CCCTTCTCAA CCAAGCATCC CAGTTTAGAT GTCCTAAGTT TCAAGATGCT 
GAACGGAAAC ACCCGGTGAT GTGCCTTCTC TGTGGGGCCA TTCTCTGTTC TCAGAATACA 
TGCTGTCAGG AGGTGGTAAA TGGAGAAGAG CTGGGAGCTT GTACAGCACA TGCCATGCAC 
TGTGGAGCTG GTGTCTGCAT CTTCCTAAGT ATCAGAGAAT GCAAAGTGGT CCTAATGGAG 
GGTAAAACCA GAGGATGTAT CTACCAGGCT CCCTATCTTG ATGAATATGG AGAAACAGAC 
CCAGGACTGA AACGTGGAAA CCCCTTACAC CTTTCCCATG AGCGATATCG GAAATTGCAT 
CAGTTGTGGC AGCAGCACTG TATTATTGAA GAGATTGGAC ACAACCTTGA AATCAACCAG 
ATGTTTTTTG ACTTTAATTG GAGCCTGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824692.2
CDS	389..5281
Translation	MDCFQNSIHKNHRYKMHSSMGGGFCDCGDTEAWKTGPYCKIHEPGPSDQNSDFRLKDELLAQTKIVFPLIIRYIVDMLIWEEQKSLPAYLDTGTVEWNQYYCVLYNDEHHTYDHFIYSLQRSLGCDQKEAQTLTSAAEREGRKAVKKGTLEQCQEVTESLKANSENVSLKPVHVEVLHGHVMAHQSFALCLAIWLNKLLAYSSDFRQIFCQVCLEQEPKPSSFNHTCYVSLLILKDSKLHKGVRKVLHDLIFSSFFMEMEYKKQFAIEFVKHYKQIQKEYINDDQDRNISVTAISVQVFTVPTLARLLIEEQNVMKVITETLLEVIKEYLGSDNKFNFQGYSQERFFMVYAIILDLKYILISKPTSWSEKLKAKFLEGFKSFLYFLSCMQGMEGISRMVGQHVEMDSDWEAVIAMQMQLKNVLTLIQEWCATDDDILLKSYKECHLSLLQCCTPVPSQEKKLIKMSGHSYQSYPYMVSKDPISIHLPLSRMLAGLHAQMCRTGVITRLCEILPPEDLQVELMIEHPLRCLVLVGQVAAEMWRRNGLTIVSQVFYYHDVKCREEMYDKDVVMLQIGASYLDPNSFLLLLLQRYELEEAFSNVAVPTKDMDVARQHNILIEEMLHVLICVIGERYVPGISNVTREECTMREIIHLLCIEPMAHSAIAKALPETENTDICLDKLISKVATFKKPGVSGHGVYELKAEYLDQFNVFFYHYTKTQHSKAEHTQKKRRKQENSSEALAPPAPPNFSPAFCNVTKLLCCDIMMHILRTILKRAAEEDPIMWTEGMIQMVLHIIALGLLEEKQQLQRAAEEEVTFNFYHKATRVRSTDANAQSVATLLERLKGITQLDAQKDMIIWVRQVFETVKGLRETSSHTVPTDTVSDVAKAEEGQSPQEKEKRKRKAEAARLHRQKIMAQMSALQKNFIETNKLLYDTTVEVQESTSPVEESLLSADEGSKVALGTKRGPTVPSKDVLTCILCQEEQEVKLDKPTMVLTACVQKSTTLSQTRSKVVSHTGETFDPLFTHPEMLYGTHTGSCGHVMHVVCWQKFFEAMQKNTRQRLHVELIFDLGNGEYLCPLCKCHCNTVIPIIPHVAQQIQSVDAEAINCVFSLPHWLDIVAARISGYNLNTIKGTKPITPEFCKIYKDQNYEFRSILSFGVQAPPRYPTCTEGMLVHFATTVYRVGLNVPPNESDSRMPVMTWNTCAFTIQCIESWLQDEEKPLFGSLQNRQLAGLQALVKFAEAQRVTCPQPLIRRHLIRLLAAFLPNITLEDTPSFLSLDVFHLLVGSVLAFPSLYCEENVDLHPSTLVSTYNNLYLFHLLTMTHILQIIFSLTTDNVPSTEPDDCEEARTAATLCQDISQLTSNSLKIDFPGWYVWKCVKEGIMPYLRCAALFFHYLLGVMPPEELKSSGTTDNQLSALCSFLCLPTNILLLFQENWDIVKLLIQRLSSDQSLQSVLAGYTTALRYPRKRNTLIELPEDYSSLLNQASQFRCPKFQDAERKHPVMCLLCGAILCSQNTCCQEVVNGEELGACTAHAMHCGAGVCIFLSIRECKVVLMEGKTRGCIYQAPYLDEYGETDPGLKRGNPLHLSHERYRKLHQLWQQHCIIEEIGHNLEINQMFFDFNWSLL

Target ORF information:

RefSeq Version	XM_012969238.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969238.3

ORF Insert Sequence:

ATGGATTGTT TCCAGAACAG CATTCATAAA AATCACAGAT ACAAGATGCA CTCTTCAATG 
GGAGGGGGAT TTTGTGACTG TGGGGATACT GAAGCCTGGA AAACCGGCCC ATATTGTAAG 
ATCCATGAAC CAGGGCCATC AGATCAGAAT TCAGACTTTC GTTTGAAGGA TGAACTTCTG 
GCCCAAACCA AAATAGTATT CCCTTTGATT ATCCGGTATA TAGTAGACAT GTTAATCTGG 
GAAGAACAAA AGAGTTTGCC AGCATATCTG GATACAGGGA CAGTGGAGTG GAACCAGTAC 
TACTGTGTAC TTTACAATGA TGAACACCAC ACCTATGATC ACTTCATCTA CAGTTTGCAG 
AGATCGCTTG GTTGTGATCA GAAAGAAGCA CAGACATTAA CCAGTGCAGC TGAGAGAGAG 
GGCCGCAAAG CTGTTAAAAA GGGTACTTTA GAGCAATGCC AGGAGGTGAC AGAAAGTCTA 
AAGGCTAATT CAGAGAACGT GTCGCTGAAA CCTGTCCACG TGGAGGTGCT GCATGGTCAT 
GTAATGGCCC ATCAAAGCTT TGCACTGTGT TTAGCAATCT GGCTTAACAA ACTCCTGGCC 
TACTCTAGTG ACTTCCGACA GATCTTCTGT CAAGTGTGCC TAGAGCAAGA GCCAAAGCCT 
AGCAGTTTTA ATCACACTTG TTATGTCAGC TTGTTAATTT TAAAGGACTC CAAGCTTCAT 
AAAGGAGTGC GGAAAGTTCT GCATGATCTG ATCTTCAGCA GCTTTTTCAT GGAAATGGAG 
TATAAGAAAC AATTTGCTAT AGAGTTTGTT AAGCATTACA AGCAGATACA GAAAGAATAC 
ATAAACGACG ATCAGGACAG GAACATTTCA GTTACTGCAA TATCAGTTCA GGTTTTCACT 
GTCCCTACTT TGGCTCGCCT ACTGATAGAA GAACAAAATG TAATGAAAGT CATCACAGAG 
ACTTTACTCG AGGTCATAAA AGAATATCTT GGCTCAGACA ACAAGTTCAA CTTCCAGGGC 
TACAGCCAGG AACGCTTTTT CATGGTATAT GCCATAATCC TTGACCTCAA GTATATCCTT 
ATCTCTAAAC CTACATCCTG GTCTGAGAAG TTAAAAGCCA AGTTTCTGGA AGGATTTAAA 
TCTTTCCTCT ATTTTTTATC CTGTATGCAG GGCATGGAAG GGATAAGCAG GATGGTGGGA 
CAGCACGTTG AGATGGATTC AGACTGGGAA GCTGTAATAG CTATGCAGAT GCAACTGAAA 
AATGTCTTGA CGCTGATACA GGAATGGTGT GCAACAGATG ATGATATATT GCTCAAATCC 
TACAAGGAAT GCCATCTGAG TCTTCTGCAG TGTTGCACCC CGGTCCCTTC CCAAGAAAAG 
AAGCTGATAA AAATGAGTGG CCATTCATAC CAAAGCTATC CATACATGGT GTCCAAAGAC 
CCAATCAGCA TACACTTACC TCTCTCTCGG ATGCTTGCTG GTCTACATGC ACAGATGTGT 
AGGACTGGTG TCATCACAAG ACTGTGTGAA ATTCTTCCTC CGGAAGACTT ACAGGTGGAA 
CTAATGATTG AACACCCCTT AAGATGCCTG GTGCTGGTGG GACAGGTAGC AGCTGAGATG 
TGGAGAAGGA ATGGCCTTAC TATTGTTAGT CAGGTTTTTT ACTATCATGA TGTGAAGTGC 
AGGGAGGAGA TGTATGACAA AGATGTTGTG ATGCTACAGA TTGGTGCATC CTACCTGGAT 
CCCAACAGCT TCCTACTGCT CTTGCTGCAG AGGTATGAAC TGGAGGAGGC ATTCTCTAAT 
GTAGCTGTGC CCACCAAAGA CATGGATGTA GCAAGGCAAC ACAACATCTT AATTGAAGAA 
ATGTTACATG TCCTTATTTG TGTTATTGGT GAGCGCTATG TGCCAGGCAT TAGCAATGTT 
ACTAGAGAAG AGTGCACTAT GAGGGAGATC ATCCATCTGC TTTGTATTGA ACCTATGGCT 
CACAGTGCAA TCGCCAAGGC TTTGCCAGAG ACTGAAAACA CTGACATATG CCTGGATAAA 
CTCATCAGTA AAGTTGCCAC TTTTAAAAAA CCGGGAGTAT CAGGACATGG CGTTTATGAA 
CTAAAGGCTG AATACCTCGA TCAATTTAAT GTGTTCTTTT ATCATTACAC CAAAACACAA 
CACAGCAAGG CAGAACACAC ACAGAAGAAA AGGCGAAAAC AAGAAAACAG CTCAGAAGCA 
TTAGCCCCTC CTGCACCCCC CAATTTCAGC CCAGCTTTTT GCAACGTGAC CAAGCTTCTG 
TGCTGTGATA TTATGATGCA TATTCTACGA ACCATCTTGA AGAGAGCAGC AGAGGAAGAT 
CCAATTATGT GGACAGAAGG CATGATACAA ATGGTCCTCC ATATTATAGC CCTGGGGTTG 
TTAGAAGAGA AGCAACAGCT ACAGAGGGCT GCTGAAGAAG AAGTAACATT TAACTTCTAT 
CATAAGGCAA CAAGGGTTAG GAGTACAGAT GCAAATGCAC AAAGTGTTGC GACACTGCTG 
GAGAGGCTGA AGGGCATTAC CCAGCTGGAT GCCCAGAAAG ACATGATCAT TTGGGTGCGA 
CAAGTTTTTG AAACCGTAAA AGGGCTGAGA GAGACATCTA GTCACACAGT TCCCACAGAT 
ACAGTTTCTG ACGTTGCAAA AGCTGAAGAG GGACAAAGCC CCCAGGAAAA GGAGAAGCGC 
AAAAGAAAAG CTGAGGCGGC CCGGCTCCAC CGCCAGAAGA TCATGGCACA GATGTCAGCT 
TTACAAAAAA ATTTCATTGA AACAAATAAG CTTCTGTATG ACACCACTGT GGAAGTACAA 
GAAAGCACTA GTCCTGTAGA AGAGAGCCTG TTATCAGCAG ATGAAGGATC GAAGGTTGCC 
TTGGGGACAA AACGTGGCCC CACAGTGCCT AGCAAAGATG TTCTAACATG TATTCTATGT 
CAAGAAGAAC AGGAAGTGAA ATTGGACAAG CCTACAATGG TGCTAACTGC CTGCGTCCAA 
AAGTCCACAA CACTGTCCCA AACTCGTAGC AAAGTGGTCT CACACACAGG AGAAACGTTT 
GATCCACTTT TTACACACCC AGAGATGTTG TATGGAACCC ATACAGGCAG CTGTGGCCAT 
GTAATGCATG TTGTGTGCTG GCAGAAGTTC TTTGAGGCAA TGCAGAAGAA TACACGGCAG 
CGTCTTCACG TGGAGCTAAT TTTTGATCTT GGAAATGGCG AATATCTTTG TCCGCTATGC 
AAATGCCACT GTAACACCGT TATACCTATA ATCCCTCATG TTGCCCAGCA AATACAAAGT 
GTGGATGCTG AAGCCATAAA TTGTGTTTTC TCACTTCCTC ATTGGTTGGA CATAGTGGCG 
GCCAGAATAT CTGGTTATAA TTTAAACACC ATCAAAGGAA CAAAGCCAAT CACTCCTGAA 
TTCTGTAAAA TTTATAAAGA CCAGAATTAT GAGTTCCGTT CCATCTTGAG CTTTGGCGTG 
CAGGCTCCGC CCCGCTATCC AACTTGCACA GAAGGAATGC TTGTTCACTT TGCCACTACA 
GTGTACCGCG TGGGCTTAAA TGTTCCTCCC AATGAGTCTG ATTCACGTAT GCCTGTCATG 
ACATGGAATA CTTGTGCATT TACAATCCAG TGTATAGAAA GCTGGCTTCA GGATGAAGAA 
AAACCCTTGT TTGGATCCCT GCAAAACCGG CAACTTGCTG GATTACAAGC CTTGGTGAAA 
TTTGCTGAGG CCCAAAGAGT TACATGCCCT CAGCCTTTGA TACGGAGACA TCTTATCCGG 
TTATTAGCAG CATTCCTGCC AAACATTACC CTTGAAGACA CCCCATCCTT TCTGTCTTTG 
GATGTTTTCC ATCTGCTGGT GGGGTCAGTG TTGGCATTCC CATCATTATA TTGTGAAGAA 
AATGTAGACT TACATCCTTC TACCCTTGTT TCTACCTACA ACAACCTCTA CCTCTTTCAC 
CTGCTCACAA TGACGCACAT ACTGCAAATC ATTTTCTCTT TAACTACAGA TAATGTGCCT 
TCTACAGAAC CTGACGACTG TGAAGAAGCC CGTACAGCTG CTACTCTTTG TCAGGACATT 
TCACAACTCA CCAGCAACAG TTTAAAGATT GATTTTCCGG GGTGGTATGT TTGGAAATGT 
GTGAAAGAGG GCATCATGCC ATATCTGCGA TGTGCAGCTT TGTTCTTCCA TTATTTGCTG 
GGAGTAATGC CTCCAGAGGA GCTAAAATCA TCAGGCACTA CAGACAATCA GTTATCAGCT 
CTTTGCAGCT TCCTCTGCTT GCCAACCAAC ATTCTTCTTC TTTTCCAAGA GAACTGGGAC 
ATTGTGAAAC TTCTCATTCA GAGGTTGAGT TCTGACCAAT CCCTGCAGAG TGTCCTGGCT 
GGTTACACTA CTGCACTCAG GTATCCAAGG AAACGCAATA CACTAATAGA GCTTCCAGAA 
GATTACAGTT CCCTTCTCAA CCAAGCATCC CAGTTTAGAT GTCCTAAGTT TCAAGATGCT 
GAACGGAAAC ACCCGGTGAT GTGCCTTCTC TGTGGGGCCA TTCTCTGTTC TCAGAATACA 
TGCTGTCAGG AGGTGGTAAA TGGAGAAGAG CTGGGAGCTT GTACAGCACA TGCCATGCAC 
TGTGGAGCTG GTGTCTGCAT CTTCCTAAGT ATCAGAGAAT GCAAAGTGGT CCTAATGGAG 
GGTAAAACCA GAGGATGTAT CTACCAGGCT CCCTATCTTG ATGAATATGG AGAAACAGAC 
CCAGGACTGA AACGTGGAAA CCCCTTACAC CTTTCCCATG AGCGATATCG GAAATTGCAT 
CAGTTGTGGC AGCAGCACTG TATTATTGAA GAGATTGGAC ACAACCTTGA AATCAACCAG 
ATGTTTTTTG ACTTTAATTG GAGCCTGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr1 ( XM_002941086.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941132.2 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X1, mRNA.

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CloneID	OXa14964
Clone ID Related Accession (Same CDS sequence)	XM_002941086.4
Accession Version	XM_002941086.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002941086.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCTG GAGGTGGAGG ATTGGAGCAA AGCAGGGAGC CAGGGGATGG AGAAATGCTT 
GCCATGAGAT TAGTCAACGC TTTGGATTTT GCCTCTGCCT TTCACCAACA CGTGGCAAAG 
CATGTCCCCC AGATTTATAC AGATGAGCTG CAGCCACAAG TGGAGAAGCA AGAGGAGCTG 
GCACAGAAAC TTATTTTAAC CCCACTGGAG TGGTGCCTCT TTAGAGAAGA TCCAAATGTA 
TTCAAGGAGA AGTTACGGCA CAGTGCAGCT CCTCCATTAT GTGGAAGAGT CTTTAGAGGA 
GGGGAGACAA CCTATTCCTG CAGGGATTGT GCCATTGATC CAACCTGTGT CCTGTGTATG 
GATTGTTTCC AGAACAGCAT TCATAAAAAT CACAGATACA AGATGCACTC TTCAATGGGA 
GGGGGATTTT GTGACTGTGG GGATACTGAA GCCTGGAAAA CCGGCCCATA TTGTAAGATC 
CATGAACCAG GGGCATCAGA TCAGAATTCA GACTTTCGTT TGAAGGATGA ACTTCTGGCC 
CAAACCAAAA TAGTATTCCC TTTGATTATC CGGTATATAG TAGACATGTT AATCTGGGAA 
GAACAAAAGA GTTTGCCAGC ATATCTGGAT ACAGGGACAG TGGAGTGGAA CCAGTACTAC 
TGTGTACTTT ACAATGATGA ACACCACACC TATGATCACT TCATCTACAG TTTGCAGAGA 
TCGCTTGGTT GTGATCAGAA AGAAGCACAG ACATTAACCA GTGCAGCTGA GAGAGAGGGC 
CGCAAAGCTG TTAAAAAGGG TACTTTAGAG CAATGCCAGG AGGTGACAGA AAGTCTAAAG 
GCTAATTCAG AGAACGTGTC GCTGAAACCT GTCCACGTGG AGGTGCTGCA TGGTCATGTA 
ATGGCCCATC AAAGCTTTGC ACTGTGTTTA GCAATCTGGC TTAACAAACT CCTGGCCTAC 
TCTAGTGACT TCCGACAGAT CTTCTGTCAA GTGTGCCTAG AGCAAGAGCC AAAGCCTAGC 
AGTTTTAATC ACACTTGTTA TGTCAGCTTG TTAATTTTAA AGGACTCCAA GCTTCATAAA 
GGAGTGCGGA AAGTTCTGCA TGATCTGATC TTCAGCAGCT TTTTCATGGA AATGGAGTAT 
AAGAAACAAT TTGCTATAGA GTTTGTTAAG CATTACAAGC AGATACAGAA AGAATACATA 
AACGACGATC AGGACAGGAA CATTTCAGTT ACTGCAATAT CAGTTCAGGT TTTCACTGTC 
CCTACTTTGG CTCGCCTACT GATAGAAGAA CAAAATGTAA TGAAAGTCAT CACAGAGACT 
TTACTCGAGG TCATAAAAGA ATATCTTGGC TCAGACAACA AGTTCAACTT CCAGGGCTAC 
AGCCAGGAAC GCTTTTTCAT GGTATATGCC ATAATCCTTG ACCTCAAGTA TATCCTTATC 
TCTAAACCTA CATCCTGGTC TGAGAAGTTA AAAGCCAAGT TTCTGGAAGG ATTTAAATCT 
TTCCTCTATT TTTTATCCTG TATGCAGGGC ATGGAAGGGA TAAGCAGGAT GGTGGGACAG 
CACGTTGAGA TGGATTCAGA CTGGGAAGCT GTAATAGCTA TGCAGATGCA ACTGAAAAAT 
GTCTTGACGC TGATACAGGA GTGGTGTGCA ACAGATGATG ATATATTGCT CAAATCCTAC 
AAGGAATGCC ATCTGAGTCT TCTGCAGTGT TGCACCCCGG TCCCTTCCCA AGAAAAGAAG 
CTGATAAAAA TGAGTGGCCA TTCATACCAA AGCTATCCAT ACATGGTGTC CAAAGACCCA 
ATCAGCATAC ACCTACCTCT CTCTCGGATG CTTGCTGGTC TACATGCACA GATGTGTAGG 
ACTGGTGTCA TCACAAGACT GTGTGAAATT CTTCCTCCGG AAGACTTACA GGTGGAACTA 
ATGATTGAAC ACCCCTTAAG ATGCCTGGTG CTGGTGGGAC AGGTAGCAGC TGAGATGTGG 
AGAAGGAATG GCCTTACTAT TGTTAGTCAG GTTTTTTACT ATCATGATGT GAAGTGCAGG 
GAGGAGATGT ATGACAAAGA TGTTGTGATG CTACAGATTG GTGCATCCTA CCTGGATCCC 
AACAGCTTCC TACTGCTCTT GCTGCAGAGG TATGAACTGG AGGAGGCATT CTCTAATGTA 
GCTGTGCCCA CCAAAGACAT GGATGTAGCA AGGCAACACA ACATCTTAAT TGAAGAAATG 
TTACATGTCC TTATTTGTGT TATTGGTGAG CGCTATGTGC CAGGCATTAG CAATGTTACT 
AGAGAAGAGT GCACTATGAG GGAGATCATC CATCTGCTTT GTATTGAACC TATGGCTCAC 
AGTGCAATCG CCAAGGCTTT GCCAGAGACT GAAAACACTG ACATATGCCT GGATAAACTC 
ATCAGTAAAG TTGCCACTTT TAAAAAACCG GGAGTATCAG GACATGGCGT TTATGAACTA 
AAGGCTGAAT ACCTCGATCA ATTTAATGTG TTCTTTTATC ATTACACCAA AACACAACAC 
AGCAAGGCAG AACACACACA GAAGAAAAGG CGAAAACAAG AAAACAGCTC AGAAGCATTA 
GCCCCTCCTG CACCCCCCAA TTTCAGCCCA GCTTTTTGCA ACGTGACCAA GCTTCTGTGC 
TGTGATATTA TGATGCATAT TCTACGAACC ATCTTGAAGA GAGCAGCAGA GGAAGATCCA 
ATTATGTGGA CAGAAGGCAT GATACAAATG GTCCTCCATA TTATAGCCCT GGGGTTATTA 
GAAGAGAAGC AACAGCTACA GAGGGCTGCT GAAGAAGAAG TAACATTTAA CTTCTATCAT 
AAGGCAACAA GGGTTAGGAG TACAGATGCA AATGCACAAA GTGTTGCGAC ACTGCTGGAG 
AAGCTGAAGG GCATTACCCA GCTGGATGCC CAGAAAGACA TGATCATTTG GGTGCGACAA 
GTTTTTGAAA CCGTAAAAGG GCTGAGAGAG ACATCTAGTC ACACAGTTCC CACAGATACA 
GTTTCTGACG TTGCAAAAGC TGAAGAGGGA CAAAGCCCCC AGGAAAAGGA GAAGCGCAAA 
AGAAAAGCTG AGGCGGCCCG GCTCCACCGC CAGAAGATCA TGGCACAGAT GTCAGCTTTA 
CAAAAAAATT TCATTGAAAC AAATAAGCTT CTGTATGACA CCACTGTGGA AGTACAAGAA 
AGCACTAGTC CTGTAGAAGA GAGCCTGTTA TCAGCAGATG AAGGATCGAA GGTTGCCTTG 
GGGACAAAAC GTGGCCCCAC AGTGCCTAGC AAAGATGTTC TAACATGTAT TCTATGTCAA 
GAAGAACAGG AAGTGAAATT GGACAAGCCT ACAATGGTGC TAACTGCCTG CGTCCAAAAG 
TCCACAACAC TGTCCCAAAC TCGTAGCAAA GTGGTCTCAC ACACAGGAGA AACGTTTGAT 
CCACTTTTTA CACACCCAGA GATGTTGTAT GGAACCCATA CAGGCAGCTG TGGCCATGTA 
ATGCATGTTG TGTGCTGGCA GAAGTTCTTT GAGGCAATGC AGAAGAATAC ACGGCAGCGT 
CTTCACGTGG AGCTAATTTT TGATCTTGGA AATGGCGAAT ATCTTTGTCC GCTATGCAAA 
TGCCACTGTA ACACCGTTAT ACCTATAATC CCTCATGTTG CCCAGCAAAT ACAAAGTGTG 
GATGCTGAAG CCATAAATTG TGTTTTCTCA CTTCCTCATT GGTTGGACAT AGTGGCGGCC 
AGAATATCTG GTTATAATTT AAACACCATC AAAGGAACAA AGCCAATCAC TCCTGAATTC 
TGTAAAATTT ATAAAGACCA GAATTATGAG TTCCGTTCCA TCTTGAGCTT TGGCGTGCAG 
GCTCCGCCCC GCTATCCAAC TTGCACAGAA GGAATGCTTG TTCACTTTGC CACTACAGTG 
TACCGCGTGG GCTTAAATGT TCCTCCCAAT GAGTCTGATC CACGTATGCC TGTCATGACA 
TGGAATACTT GTGCATTTAC AATCCAGTGT ATAGAAAGCT GGCTTCAGGA TGAAGAAAAA 
CCCTTGTTTG GATCCCTGCA AAACCGGCAA CTTGCTGGAT TACAAGCCTT GGTGAAATTT 
GCTGAGGCCC AAAGAGTTAC ATGCCCTCAG CCTTTGATAC GGAGACATCT TATCCGGTTA 
TTAGCAGCAT TCCTGCCAAA CATTACCCTT GAAGACACCC CATCCTTTCT GTCTTTGGAT 
GTTTTCCATC TGCTGGTGGG GTCAGTGTTG GCATTCCCAT CATTATATTG TGAAGAAAAT 
GTAGACTTAC ATCCTTCTAC CCTTGTTTCT ACCTACAACA ACCTCTACCT CTTTCACCTG 
CTCACAATGA CGCACATACT GCAAATCATT TTCTCTTTAA CTACAGATAA TGTGCCTTCT 
ACAGAACCTG ACGACTGTGA AGAAGCCCGT ACAGCTGCTA CTCTTTGTCA GGACATTTCA 
CAACTCACCA GCAACAGTTT AAAGATTGAT TTTCCGGGGT GGTATGTTTG GAAATGTGTG 
AAAGAGGGCA TCATGCCATA TCTGCGATGT GCAGCTTTGT TCTTCCATTA TTTGCTGGGA 
GTAATGCCTC CAGAGGAGCT AAAATCATCA GGCACTACAG ACAATCAGTT ATCAGCTCTT 
TGCAGCTTCC TCTGCTTGCC AACCAACATT CTTCTTCTTT TCCAAGAGAA CTGGGACATT 
GTGAAACTTC TCATTCAGAG GTTGAGTTCT GACCAATCCC TGCAGAGTGT CCTGGCTGGT 
TACACTACTG CACTCAGGTA TCCAAGGAAA CGCAATACAC TAATAGAGCT TCCAGAAGAT 
TACAGTTCCC TTCTCAACCA AGCATCCCAG TTTAGATGTC CTAAGTCTCA AGATGCTGAA 
CGGAAACACC CGGTTATGTG CCTTCTCTGT GGGGCCATTC TCTGTTCTCA GAATACATGC 
TGTCAGGAGG TGGTAAATGG AGAAGAGCTG GGAGCTTGTA CAGCACATGC CATGCACTGT 
GGAGCTGGTG TCTGCATCTT CCTAAGTATC AGAGAATGCA AAGTGGTCCT AATGGAGGGT 
AAAACCAGAG GATGTATCTA CCAGGCTCCC TATCTTGATG AATATGGAGA AACAGACCCA 
GGACTGAAAC GTGGAAACCC CTTACACCTT TCCCATGAGC GATATCGGAA ATTGCATCAG 
TTGTGGCAGC AGCACTGTAT TATTGAAGAG ATTGGACACA ACCTTGAAAT CAACCAGATG 
TTTTTTGACT TTAATTGGAG CCTGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941132.2
CDS	468..5717
Translation	MESGGGGLEQSREPGDGEMLAMRLVNALDFASAFHQHVAKHVPQIYTDELQPQVEKQEELAQKLILTPLEWCLFREDPNVFKEKLRHSAAPPLCGRVFRGGETTYSCRDCAIDPTCVLCMDCFQNSIHKNHRYKMHSSMGGGFCDCGDTEAWKTGPYCKIHEPGASDQNSDFRLKDELLAQTKIVFPLIIRYIVDMLIWEEQKSLPAYLDTGTVEWNQYYCVLYNDEHHTYDHFIYSLQRSLGCDQKEAQTLTSAAEREGRKAVKKGTLEQCQEVTESLKANSENVSLKPVHVEVLHGHVMAHQSFALCLAIWLNKLLAYSSDFRQIFCQVCLEQEPKPSSFNHTCYVSLLILKDSKLHKGVRKVLHDLIFSSFFMEMEYKKQFAIEFVKHYKQIQKEYINDDQDRNISVTAISVQVFTVPTLARLLIEEQNVMKVITETLLEVIKEYLGSDNKFNFQGYSQERFFMVYAIILDLKYILISKPTSWSEKLKAKFLEGFKSFLYFLSCMQGMEGISRMVGQHVEMDSDWEAVIAMQMQLKNVLTLIQEWCATDDDILLKSYKECHLSLLQCCTPVPSQEKKLIKMSGHSYQSYPYMVSKDPISIHLPLSRMLAGLHAQMCRTGVITRLCEILPPEDLQVELMIEHPLRCLVLVGQVAAEMWRRNGLTIVSQVFYYHDVKCREEMYDKDVVMLQIGASYLDPNSFLLLLLQRYELEEAFSNVAVPTKDMDVARQHNILIEEMLHVLICVIGERYVPGISNVTREECTMREIIHLLCIEPMAHSAIAKALPETENTDICLDKLISKVATFKKPGVSGHGVYELKAEYLDQFNVFFYHYTKTQHSKAEHTQKKRRKQENSSEALAPPAPPNFSPAFCNVTKLLCCDIMMHILRTILKRAAEEDPIMWTEGMIQMVLHIIALGLLEEKQQLQRAAEEEVTFNFYHKATRVRSTDANAQSVATLLEKLKGITQLDAQKDMIIWVRQVFETVKGLRETSSHTVPTDTVSDVAKAEEGQSPQEKEKRKRKAEAARLHRQKIMAQMSALQKNFIETNKLLYDTTVEVQESTSPVEESLLSADEGSKVALGTKRGPTVPSKDVLTCILCQEEQEVKLDKPTMVLTACVQKSTTLSQTRSKVVSHTGETFDPLFTHPEMLYGTHTGSCGHVMHVVCWQKFFEAMQKNTRQRLHVELIFDLGNGEYLCPLCKCHCNTVIPIIPHVAQQIQSVDAEAINCVFSLPHWLDIVAARISGYNLNTIKGTKPITPEFCKIYKDQNYEFRSILSFGVQAPPRYPTCTEGMLVHFATTVYRVGLNVPPNESDPRMPVMTWNTCAFTIQCIESWLQDEEKPLFGSLQNRQLAGLQALVKFAEAQRVTCPQPLIRRHLIRLLAAFLPNITLEDTPSFLSLDVFHLLVGSVLAFPSLYCEENVDLHPSTLVSTYNNLYLFHLLTMTHILQIIFSLTTDNVPSTEPDDCEEARTAATLCQDISQLTSNSLKIDFPGWYVWKCVKEGIMPYLRCAALFFHYLLGVMPPEELKSSGTTDNQLSALCSFLCLPTNILLLFQENWDIVKLLIQRLSSDQSLQSVLAGYTTALRYPRKRNTLIELPEDYSSLLNQASQFRCPKSQDAERKHPVMCLLCGAILCSQNTCCQEVVNGEELGACTAHAMHCGAGVCIFLSIRECKVVLMEGKTRGCIYQAPYLDEYGETDPGLKRGNPLHLSHERYRKLHQLWQQHCIIEEIGHNLEINQMFFDFNWSLL

Target ORF information:

RefSeq Version	XM_002941086.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002941086.4

ORF Insert Sequence:

ATGGAGTCTG GAGGTGGAGG ATTGGAGCAA AGCAGGGAGC CAGGGGATGG AGAAATGCTT 
GCCATGAGAT TAGTCAACGC TTTGGATTTT GCCTCTGCCT TTCACCAACA CGTGGCAAAG 
CATGTCCCCC AGATTTATAC AGATGAGCTG CAGCCACAAG TGGAGAAGCA AGAGGAGCTG 
GCACAGAAAC TTATTTTAAC CCCACTGGAG TGGTGCCTCT TTAGAGAAGA TCCAAATGTA 
TTCAAGGAGA AGTTACGGCA CAGTGCAGCT CCTCCATTAT GTGGAAGAGT CTTTAGAGGA 
GGGGAGACAA CCTATTCCTG CAGGGATTGT GCCATTGATC CAACCTGTGT CCTGTGTATG 
GATTGTTTCC AGAACAGCAT TCATAAAAAT CACAGATACA AGATGCACTC TTCAATGGGA 
GGGGGATTTT GTGACTGTGG GGATACTGAA GCCTGGAAAA CCGGCCCATA TTGTAAGATC 
CATGAACCAG GGGCATCAGA TCAGAATTCA GACTTTCGTT TGAAGGATGA ACTTCTGGCC 
CAAACCAAAA TAGTATTCCC TTTGATTATC CGGTATATAG TAGACATGTT AATCTGGGAA 
GAACAAAAGA GTTTGCCAGC ATATCTGGAT ACAGGGACAG TGGAGTGGAA CCAGTACTAC 
TGTGTACTTT ACAATGATGA ACACCACACC TATGATCACT TCATCTACAG TTTGCAGAGA 
TCGCTTGGTT GTGATCAGAA AGAAGCACAG ACATTAACCA GTGCAGCTGA GAGAGAGGGC 
CGCAAAGCTG TTAAAAAGGG TACTTTAGAG CAATGCCAGG AGGTGACAGA AAGTCTAAAG 
GCTAATTCAG AGAACGTGTC GCTGAAACCT GTCCACGTGG AGGTGCTGCA TGGTCATGTA 
ATGGCCCATC AAAGCTTTGC ACTGTGTTTA GCAATCTGGC TTAACAAACT CCTGGCCTAC 
TCTAGTGACT TCCGACAGAT CTTCTGTCAA GTGTGCCTAG AGCAAGAGCC AAAGCCTAGC 
AGTTTTAATC ACACTTGTTA TGTCAGCTTG TTAATTTTAA AGGACTCCAA GCTTCATAAA 
GGAGTGCGGA AAGTTCTGCA TGATCTGATC TTCAGCAGCT TTTTCATGGA AATGGAGTAT 
AAGAAACAAT TTGCTATAGA GTTTGTTAAG CATTACAAGC AGATACAGAA AGAATACATA 
AACGACGATC AGGACAGGAA CATTTCAGTT ACTGCAATAT CAGTTCAGGT TTTCACTGTC 
CCTACTTTGG CTCGCCTACT GATAGAAGAA CAAAATGTAA TGAAAGTCAT CACAGAGACT 
TTACTCGAGG TCATAAAAGA ATATCTTGGC TCAGACAACA AGTTCAACTT CCAGGGCTAC 
AGCCAGGAAC GCTTTTTCAT GGTATATGCC ATAATCCTTG ACCTCAAGTA TATCCTTATC 
TCTAAACCTA CATCCTGGTC TGAGAAGTTA AAAGCCAAGT TTCTGGAAGG ATTTAAATCT 
TTCCTCTATT TTTTATCCTG TATGCAGGGC ATGGAAGGGA TAAGCAGGAT GGTGGGACAG 
CACGTTGAGA TGGATTCAGA CTGGGAAGCT GTAATAGCTA TGCAGATGCA ACTGAAAAAT 
GTCTTGACGC TGATACAGGA GTGGTGTGCA ACAGATGATG ATATATTGCT CAAATCCTAC 
AAGGAATGCC ATCTGAGTCT TCTGCAGTGT TGCACCCCGG TCCCTTCCCA AGAAAAGAAG 
CTGATAAAAA TGAGTGGCCA TTCATACCAA AGCTATCCAT ACATGGTGTC CAAAGACCCA 
ATCAGCATAC ACCTACCTCT CTCTCGGATG CTTGCTGGTC TACATGCACA GATGTGTAGG 
ACTGGTGTCA TCACAAGACT GTGTGAAATT CTTCCTCCGG AAGACTTACA GGTGGAACTA 
ATGATTGAAC ACCCCTTAAG ATGCCTGGTG CTGGTGGGAC AGGTAGCAGC TGAGATGTGG 
AGAAGGAATG GCCTTACTAT TGTTAGTCAG GTTTTTTACT ATCATGATGT GAAGTGCAGG 
GAGGAGATGT ATGACAAAGA TGTTGTGATG CTACAGATTG GTGCATCCTA CCTGGATCCC 
AACAGCTTCC TACTGCTCTT GCTGCAGAGG TATGAACTGG AGGAGGCATT CTCTAATGTA 
GCTGTGCCCA CCAAAGACAT GGATGTAGCA AGGCAACACA ACATCTTAAT TGAAGAAATG 
TTACATGTCC TTATTTGTGT TATTGGTGAG CGCTATGTGC CAGGCATTAG CAATGTTACT 
AGAGAAGAGT GCACTATGAG GGAGATCATC CATCTGCTTT GTATTGAACC TATGGCTCAC 
AGTGCAATCG CCAAGGCTTT GCCAGAGACT GAAAACACTG ACATATGCCT GGATAAACTC 
ATCAGTAAAG TTGCCACTTT TAAAAAACCG GGAGTATCAG GACATGGCGT TTATGAACTA 
AAGGCTGAAT ACCTCGATCA ATTTAATGTG TTCTTTTATC ATTACACCAA AACACAACAC 
AGCAAGGCAG AACACACACA GAAGAAAAGG CGAAAACAAG AAAACAGCTC AGAAGCATTA 
GCCCCTCCTG CACCCCCCAA TTTCAGCCCA GCTTTTTGCA ACGTGACCAA GCTTCTGTGC 
TGTGATATTA TGATGCATAT TCTACGAACC ATCTTGAAGA GAGCAGCAGA GGAAGATCCA 
ATTATGTGGA CAGAAGGCAT GATACAAATG GTCCTCCATA TTATAGCCCT GGGGTTATTA 
GAAGAGAAGC AACAGCTACA GAGGGCTGCT GAAGAAGAAG TAACATTTAA CTTCTATCAT 
AAGGCAACAA GGGTTAGGAG TACAGATGCA AATGCACAAA GTGTTGCGAC ACTGCTGGAG 
AAGCTGAAGG GCATTACCCA GCTGGATGCC CAGAAAGACA TGATCATTTG GGTGCGACAA 
GTTTTTGAAA CCGTAAAAGG GCTGAGAGAG ACATCTAGTC ACACAGTTCC CACAGATACA 
GTTTCTGACG TTGCAAAAGC TGAAGAGGGA CAAAGCCCCC AGGAAAAGGA GAAGCGCAAA 
AGAAAAGCTG AGGCGGCCCG GCTCCACCGC CAGAAGATCA TGGCACAGAT GTCAGCTTTA 
CAAAAAAATT TCATTGAAAC AAATAAGCTT CTGTATGACA CCACTGTGGA AGTACAAGAA 
AGCACTAGTC CTGTAGAAGA GAGCCTGTTA TCAGCAGATG AAGGATCGAA GGTTGCCTTG 
GGGACAAAAC GTGGCCCCAC AGTGCCTAGC AAAGATGTTC TAACATGTAT TCTATGTCAA 
GAAGAACAGG AAGTGAAATT GGACAAGCCT ACAATGGTGC TAACTGCCTG CGTCCAAAAG 
TCCACAACAC TGTCCCAAAC TCGTAGCAAA GTGGTCTCAC ACACAGGAGA AACGTTTGAT 
CCACTTTTTA CACACCCAGA GATGTTGTAT GGAACCCATA CAGGCAGCTG TGGCCATGTA 
ATGCATGTTG TGTGCTGGCA GAAGTTCTTT GAGGCAATGC AGAAGAATAC ACGGCAGCGT 
CTTCACGTGG AGCTAATTTT TGATCTTGGA AATGGCGAAT ATCTTTGTCC GCTATGCAAA 
TGCCACTGTA ACACCGTTAT ACCTATAATC CCTCATGTTG CCCAGCAAAT ACAAAGTGTG 
GATGCTGAAG CCATAAATTG TGTTTTCTCA CTTCCTCATT GGTTGGACAT AGTGGCGGCC 
AGAATATCTG GTTATAATTT AAACACCATC AAAGGAACAA AGCCAATCAC TCCTGAATTC 
TGTAAAATTT ATAAAGACCA GAATTATGAG TTCCGTTCCA TCTTGAGCTT TGGCGTGCAG 
GCTCCGCCCC GCTATCCAAC TTGCACAGAA GGAATGCTTG TTCACTTTGC CACTACAGTG 
TACCGCGTGG GCTTAAATGT TCCTCCCAAT GAGTCTGATC CACGTATGCC TGTCATGACA 
TGGAATACTT GTGCATTTAC AATCCAGTGT ATAGAAAGCT GGCTTCAGGA TGAAGAAAAA 
CCCTTGTTTG GATCCCTGCA AAACCGGCAA CTTGCTGGAT TACAAGCCTT GGTGAAATTT 
GCTGAGGCCC AAAGAGTTAC ATGCCCTCAG CCTTTGATAC GGAGACATCT TATCCGGTTA 
TTAGCAGCAT TCCTGCCAAA CATTACCCTT GAAGACACCC CATCCTTTCT GTCTTTGGAT 
GTTTTCCATC TGCTGGTGGG GTCAGTGTTG GCATTCCCAT CATTATATTG TGAAGAAAAT 
GTAGACTTAC ATCCTTCTAC CCTTGTTTCT ACCTACAACA ACCTCTACCT CTTTCACCTG 
CTCACAATGA CGCACATACT GCAAATCATT TTCTCTTTAA CTACAGATAA TGTGCCTTCT 
ACAGAACCTG ACGACTGTGA AGAAGCCCGT ACAGCTGCTA CTCTTTGTCA GGACATTTCA 
CAACTCACCA GCAACAGTTT AAAGATTGAT TTTCCGGGGT GGTATGTTTG GAAATGTGTG 
AAAGAGGGCA TCATGCCATA TCTGCGATGT GCAGCTTTGT TCTTCCATTA TTTGCTGGGA 
GTAATGCCTC CAGAGGAGCT AAAATCATCA GGCACTACAG ACAATCAGTT ATCAGCTCTT 
TGCAGCTTCC TCTGCTTGCC AACCAACATT CTTCTTCTTT TCCAAGAGAA CTGGGACATT 
GTGAAACTTC TCATTCAGAG GTTGAGTTCT GACCAATCCC TGCAGAGTGT CCTGGCTGGT 
TACACTACTG CACTCAGGTA TCCAAGGAAA CGCAATACAC TAATAGAGCT TCCAGAAGAT 
TACAGTTCCC TTCTCAACCA AGCATCCCAG TTTAGATGTC CTAAGTCTCA AGATGCTGAA 
CGGAAACACC CGGTTATGTG CCTTCTCTGT GGGGCCATTC TCTGTTCTCA GAATACATGC 
TGTCAGGAGG TGGTAAATGG AGAAGAGCTG GGAGCTTGTA CAGCACATGC CATGCACTGT 
GGAGCTGGTG TCTGCATCTT CCTAAGTATC AGAGAATGCA AAGTGGTCCT AATGGAGGGT 
AAAACCAGAG GATGTATCTA CCAGGCTCCC TATCTTGATG AATATGGAGA AACAGACCCA 
GGACTGAAAC GTGGAAACCC CTTACACCTT TCCCATGAGC GATATCGGAA ATTGCATCAG 
TTGTGGCAGC AGCACTGTAT TATTGAAGAG ATTGGACACA ACCTTGAAAT CAACCAGATG 
TTTTTTGACT TTAATTGGAG CCTGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr1 ( XM_012969237.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824691.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X2, mRNA.

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CloneID	OXa34672
Clone ID Related Accession (Same CDS sequence)	XM_012969237.2
Accession Version	XM_012969237.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCTG GAGGTGGAGG ATTGGAGCAA AGCAGGGAGC CAGGGGATGG AGAAATGCTT 
GCCATGAGAT TAGTCAACGC TTTGGATTTT GCCTCTGCCT TTCACCAACA CGTGGCAAAG 
CATGTCCCCC AGATTTATAC AGATGAGCTG CAGCCACAAG TGGAGAAGCA AGAGGAGCTG 
GCACAGAAAC TTATTTTAAC CCCACTGGAG TGGTGCCTCT TTAGAGAAGA TCCAAATGTA 
TTCAAGGAGA AGTTACGGCA CAGTGCAGCT CCTCCATTAT GTGGAAGAGT CTTTAGAGGA 
GGGGAGACAA CCTATTCCTG CAGGGATTGT GCCATTGATC CAACCTGTGT CCTGTGTATG 
GATTGTTTCC AGAACAGCAT TCATAAAAAT CACAGATACA AGATGCACTC TTCAATGGGA 
GGGGGATTTT GTGACTGTGG GGATACTGAA GCCTGGAAAA CCGGCCCATA TTGTAAGATC 
CATGAACCAG GGGCATCAGA TCAGAATTCA GACTTTCGTT TGAAGGATGA ACTTCTGGCC 
CAAACCAAAA TAGTATTCCC TTTGATTATC CGGTATATAG TAGACATGTT AATCTGGGAA 
GAACAAAAGA GTTTGCCAGC ATATCTGGAT ACAGGGACAG TGGAGTGGAA CCAGTACTAC 
TGTGTACTTT ACAATGATGA ACACCACACC TATGATCACT TCATCTACAG TTTGCAGAGA 
TCGCTTGGTT GTGATCAGAA AGAAGCACAG ACATTAACCA GTGCAGCTGA GAGAGAGGGC 
CGCAAAGCTG TTAAAAAGGG TACTTTAGAG CAATGCCAGG AGGTGACAGA AAGTCTAAAG 
GCTAATTCAG AGAACGTGTC GCTGAAACCT GTCCACGTGG AGGTGCTGCA TGGTCATGTA 
ATGGCCCATC AAAGCTTTGC ACTGTGTTTA GCAATCTGGC TTAACAAACT CCTGGCCTAC 
TCTAGTGACT TCCGACAGAT CTTCTGTCAA GTGTGCCTAG AGCAAGAGCC AAAGCCTAGC 
AGTTTTAATC ACACTTGTTA TGTCAGCTTG TTAATTTTAA AGGACTCCAA GCTTCATAAA 
GGAGTGCGGA AAGTTCTGCA TGATCTGATC TTCAGCAGCT TTTTCATGGA AATGGAGTAT 
AAGAAACAAT TTGCTATAGA GTTTGTTAAG CATTACAAGC AGATACAGAA AGAATACATA 
AACGACGATC AGGACAGGAA CATTTCAGTT ACTGCAATAT CAGTTCAGGT TTTCACTGTC 
CCTACTTTGG CTCGCCTACT GATAGAAGAA CAAAATGTAA TGAAAGTCAT CACAGAGACT 
TTACTCGAGG TCATAAAAGA ATATCTTGGC TCAGACAACA AGTTCAACTT CCAGGGCTAC 
AGCCAGGAAC GCTTTTTCAT GGTATATGCC ATAATCCTTG ACCTCAAGTA TATCCTTATC 
TCTAAACCTA CATCCTGGTC TGAGAAGTTA AAAGCCAAGT TTCTGGAAGG ATTTAAATCT 
TTCCTCTATT TTTTATCCTG TATGCAGGGC ATGGAAGGGA TAAGCAGGAT GGTGGGACAG 
CACGTTGAGA TGGATTCAGA CTGGGAAGCT GTAATAGCTA TGCAGATGCA ACTGAAAAAT 
GTCTTGACGC TGATACAGGA GTGGTGTGCA ACAGATGATG ATATATTGCT CAAATCCTAC 
AAGGAATGCC ATCTGAGTCT TCTGCAGTGT TGCACCCCGG TCCCTTCCCA AGAAAAGAAG 
CTGATAAAAA TGAGTGGCCA TTCATACCAA AGCTATCCAT ACATGGTGTC CAAAGACCCA 
ATCAGCATAC ACCTACCTCT CTCTCGGATG CTTGCTGGTC TACATGCACA GATGTGTAGG 
ACTGGTGTCA TCACAAGACT GTGTGAAATT CTTCCTCCGG AAGACTTACA GGTGGAACTA 
ATGATTGAAC ACCCCTTAAG ATGCCTGGTG CTGGTGGGAC AGGTAGCAGC TGAGATGTGG 
AGAAGGAATG GCCTTACTAT TGTTAGTCAG GTTTTTTACT ATCATGATGT GAAGTGCAGG 
GAGGAGATGT ATGACAAAGA TGTTGTGATG CTACAGATTG GTGCATCCTA CCTGGATCCC 
AACAGCTTCC TACTGCTCTT GCTGCAGAGG TATGAACTGG AGGAGGCATT CTCTAATGTA 
GCTGTGCCCA CCAAAGACAT GGATGTAGCA AGGCAACACA ACATCTTAAT TGAAGAAATG 
TTACATGTCC TTATTTGTGT TATTGGTGAG CGCTATGTGC CAGGCATTAG CAATGTTACT 
AGAGAAGAGT GCACTATGAG GGAGATCATC CATCTGCTTT GTATTGAACC TATGGCTCAC 
AGTGCAATCG CCAAGGCTTT GCCAGAGACT GAAAACACTG ACATATGCCT GGATAAACTC 
ATCAGTAAAG TTGCCACTTT TAAAAAACCG GGAGTATCAG GACATGGCGT TTATGAACTA 
AAGGCTGAAT ACCTCGATCA ATTTAATGTG TTCTTTTATC ATTACACCAA AACACAACAC 
AGCAAGGCAG AACACACACA GAAGAAAAGG CGAAAACAAG AAAACAGCTC AGAAGCATTA 
GCCCCTCCTG CACCCCCCAA TTTCAGCCCA GCTTTTTGCA ACGTGACCAA GCTTCTGTGC 
TGTGATATTA TGATGCATAT TCTACGAACC ATCTTGAAGA GAGCAGCAGA GGAAGATCCA 
ATTATGTGGA CAGAAGGCAT GATACAAATG GTCCTCCATA TTATAGCCCT GGGGTTATTA 
GAAGAGAAGC AACAGCTACA GAGGGCTGCT GAAGAAGAAG TAACATTTAA CTTCTATCAT 
AAGGCAACAA GGGTTAGGAG TACAGATGCA AATGCACAAA GTGTTGCGAC ACTGCTGGAG 
AAGCTGAAGG GCATTACCCA GCTGGATGCC CAGAAAGACA TGATCATTTG GGTGCGACAA 
GTTTTTGAAA CCGTAAAAGG GCTGAGAGAG ACATCTAGTC ACACAGTTCC CACAGATACA 
GTTTCTGACG TTGCAAAAGC TGAAGAGGGA CAAAGCCCCC AGGAAAAGGA GAAGCGCAAA 
AGAAAAGCTG AGGCGGCCCG GCTCCACCGC CAGAAGATCA TGGCACAGAT GTCAGCTTTA 
CAAAAAAATT TCATTGAAAC AAATAAGCTT CTGTATGACA CCACTGTGGA AGTACAAGAA 
AGCACTAGTC CTGTAGAAGA GAGCCTGTTA TCAGCAGATG AAGGATCGAA GGTTGCCTTG 
GGGACAAAAC GTGGCCCCAC AGTGCCTAGC AAAGATGTTC TAACATGTAT TCTATGTCAA 
GAAGAACAGG AAGTGAAATT GGACAAGCCT ACAATGGTGC TAACTGCCTG CGTCCAAAAG 
TCCACAACAC TGTCCCAAAC TCGTAGCAAA GTGGTCTCAC ACACAGGAGA AACGTTTGAT 
CCACTTTTTA CACACCCAGA GATGTTGTAT GGAACCCATA CAGGCAGCTG TGGCCATGTA 
ATGCATGTTG TGTGCTGGCA GAAGTTCTTT GAGGCAATGC AGAAGAATAC ACGGCAGCGT 
CTTCACGTGG AGCTAATTTT TGATCTTGGA AATGGCGAAT ATCTTTGTCC GCTATGCAAA 
TGCCACTGTA ACACCGTTAT ACCTATAATC CCTCATGTTG CCCAGCAAAT ACAAAGTGTG 
GATGCTGAAG CCATAAATTG TGTTTTCTCA CTTCCTCATT GGTTGGACAT AGTGGCGGCC 
AGAATATCTG GTTATAATTT AAACACCATC AAAGGAACAA AGCCAATCAC TCCTGAATTC 
TGTAAAATTT ATAAAGACCA GAATTATGAG TTCCGTTCCA TCTTGAGCTT TGGCGTGCAG 
GCTCCGCCCC GCTATCCAAC TTGCACAGAA GGAATGCTTG TTCACTTTGC CACTACAGTG 
TACCGCGTGG GCTTAAATGT TCCTCCCAAT GAGTCTGATC CACGTATGCC TGTCATGACA 
TGGAATACTT GTGCATTTAC AATCCAGTGT ATAGAAAGCT GGCTTCAGGA TGAAGAAAAA 
CCCTTGTTTG GATCCCTGCA AAACCGGCAA CTTGCTGGAT TACAAGCCTT GGTGAAATTT 
GCTGAGGCCC AAAGAGTTAC ATGCCCTCAG CCTTTGATAC GGAGACATCT TATCCGGTTA 
TTAGCAGCAT TCCTGCCAAA CATTACCCTT GAAGACACCC CATCCTTTCT GTCTTTGGAT 
GTTTTCCATC TGCTGGTGGG GTCAGTGTTG GCATTCCCAT CATTATATTG TGAAGAAAAT 
GTAGACTTAC ATCCTTCTAC CCTTGTTTCT ACCTACAACA ACCTCTACCT CTTTCACCTG 
CTCACAATGA CGCACATACT GCAAATCATT TTCTCTTTAA CTACAGATAA TGTGCCTTCT 
ACAGAACCTG ACGACTGTGA AGAAGCCCGT ACAGCTGCTA CTCTTTGTCA GGACATTTCA 
CAACTCACCA GCAATTTAAA GATTGATTTT CCGGGGTGGT ATGTTTGGAA ATGTGTGAAA 
GAGGGCATCA TGCCATATCT GCGATGTGCA GCTTTGTTCT TCCATTATTT GCTGGGAGTA 
ATGCCTCCAG AGGAGCTAAA ATCATCAGGC ACTACAGACA ATCAGTTATC AGCTCTTTGC 
AGCTTCCTCT GCTTGCCAAC CAACATTCTT CTTCTTTTCC AAGAGAACTG GGACATTGTG 
AAACTTCTCA TTCAGAGGTT GAGTTCTGAC CAATCCCTGC AGAGTGTCCT GGCTGGTTAC 
ACTACTGCAC TCAGGTATCC AAGGAAACGC AATACACTAA TAGAGCTTCC AGAAGATTAC 
AGTTCCCTTC TCAACCAAGC ATCCCAGTTT AGATGTCCTA AGTCTCAAGA TGCTGAACGG 
AAACACCCGG TTATGTGCCT TCTCTGTGGG GCCATTCTCT GTTCTCAGAA TACATGCTGT 
CAGGAGGTGG TAAATGGAGA AGAGCTGGGA GCTTGTACAG CACATGCCAT GCACTGTGGA 
GCTGGTGTCT GCATCTTCCT AAGTATCAGA GAATGCAAAG TGGTCCTAAT GGAGGGTAAA 
ACCAGAGGAT GTATCTACCA GGCTCCCTAT CTTGATGAAT ATGGAGAAAC AGACCCAGGA 
CTGAAACGTG GAAACCCCTT ACACCTTTCC CATGAGCGAT ATCGGAAATT GCATCAGTTG 
TGGCAGCAGC ACTGTATTAT TGAAGAGATT GGACACAACC TTGAAATCAA CCAGATGTTT 
TTTGACTTTA ATTGGAGCCT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824691.1
CDS	468..5714
Translation	MESGGGGLEQSREPGDGEMLAMRLVNALDFASAFHQHVAKHVPQIYTDELQPQVEKQEELAQKLILTPLEWCLFREDPNVFKEKLRHSAAPPLCGRVFRGGETTYSCRDCAIDPTCVLCMDCFQNSIHKNHRYKMHSSMGGGFCDCGDTEAWKTGPYCKIHEPGASDQNSDFRLKDELLAQTKIVFPLIIRYIVDMLIWEEQKSLPAYLDTGTVEWNQYYCVLYNDEHHTYDHFIYSLQRSLGCDQKEAQTLTSAAEREGRKAVKKGTLEQCQEVTESLKANSENVSLKPVHVEVLHGHVMAHQSFALCLAIWLNKLLAYSSDFRQIFCQVCLEQEPKPSSFNHTCYVSLLILKDSKLHKGVRKVLHDLIFSSFFMEMEYKKQFAIEFVKHYKQIQKEYINDDQDRNISVTAISVQVFTVPTLARLLIEEQNVMKVITETLLEVIKEYLGSDNKFNFQGYSQERFFMVYAIILDLKYILISKPTSWSEKLKAKFLEGFKSFLYFLSCMQGMEGISRMVGQHVEMDSDWEAVIAMQMQLKNVLTLIQEWCATDDDILLKSYKECHLSLLQCCTPVPSQEKKLIKMSGHSYQSYPYMVSKDPISIHLPLSRMLAGLHAQMCRTGVITRLCEILPPEDLQVELMIEHPLRCLVLVGQVAAEMWRRNGLTIVSQVFYYHDVKCREEMYDKDVVMLQIGASYLDPNSFLLLLLQRYELEEAFSNVAVPTKDMDVARQHNILIEEMLHVLICVIGERYVPGISNVTREECTMREIIHLLCIEPMAHSAIAKALPETENTDICLDKLISKVATFKKPGVSGHGVYELKAEYLDQFNVFFYHYTKTQHSKAEHTQKKRRKQENSSEALAPPAPPNFSPAFCNVTKLLCCDIMMHILRTILKRAAEEDPIMWTEGMIQMVLHIIALGLLEEKQQLQRAAEEEVTFNFYHKATRVRSTDANAQSVATLLEKLKGITQLDAQKDMIIWVRQVFETVKGLRETSSHTVPTDTVSDVAKAEEGQSPQEKEKRKRKAEAARLHRQKIMAQMSALQKNFIETNKLLYDTTVEVQESTSPVEESLLSADEGSKVALGTKRGPTVPSKDVLTCILCQEEQEVKLDKPTMVLTACVQKSTTLSQTRSKVVSHTGETFDPLFTHPEMLYGTHTGSCGHVMHVVCWQKFFEAMQKNTRQRLHVELIFDLGNGEYLCPLCKCHCNTVIPIIPHVAQQIQSVDAEAINCVFSLPHWLDIVAARISGYNLNTIKGTKPITPEFCKIYKDQNYEFRSILSFGVQAPPRYPTCTEGMLVHFATTVYRVGLNVPPNESDPRMPVMTWNTCAFTIQCIESWLQDEEKPLFGSLQNRQLAGLQALVKFAEAQRVTCPQPLIRRHLIRLLAAFLPNITLEDTPSFLSLDVFHLLVGSVLAFPSLYCEENVDLHPSTLVSTYNNLYLFHLLTMTHILQIIFSLTTDNVPSTEPDDCEEARTAATLCQDISQLTSNLKIDFPGWYVWKCVKEGIMPYLRCAALFFHYLLGVMPPEELKSSGTTDNQLSALCSFLCLPTNILLLFQENWDIVKLLIQRLSSDQSLQSVLAGYTTALRYPRKRNTLIELPEDYSSLLNQASQFRCPKSQDAERKHPVMCLLCGAILCSQNTCCQEVVNGEELGACTAHAMHCGAGVCIFLSIRECKVVLMEGKTRGCIYQAPYLDEYGETDPGLKRGNPLHLSHERYRKLHQLWQQHCIIEEIGHNLEINQMFFDFNWSLL

Target ORF information:

RefSeq Version	XM_012969237.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969237.2

ORF Insert Sequence:

ATGGAGTCTG GAGGTGGAGG ATTGGAGCAA AGCAGGGAGC CAGGGGATGG AGAAATGCTT 
GCCATGAGAT TAGTCAACGC TTTGGATTTT GCCTCTGCCT TTCACCAACA CGTGGCAAAG 
CATGTCCCCC AGATTTATAC AGATGAGCTG CAGCCACAAG TGGAGAAGCA AGAGGAGCTG 
GCACAGAAAC TTATTTTAAC CCCACTGGAG TGGTGCCTCT TTAGAGAAGA TCCAAATGTA 
TTCAAGGAGA AGTTACGGCA CAGTGCAGCT CCTCCATTAT GTGGAAGAGT CTTTAGAGGA 
GGGGAGACAA CCTATTCCTG CAGGGATTGT GCCATTGATC CAACCTGTGT CCTGTGTATG 
GATTGTTTCC AGAACAGCAT TCATAAAAAT CACAGATACA AGATGCACTC TTCAATGGGA 
GGGGGATTTT GTGACTGTGG GGATACTGAA GCCTGGAAAA CCGGCCCATA TTGTAAGATC 
CATGAACCAG GGGCATCAGA TCAGAATTCA GACTTTCGTT TGAAGGATGA ACTTCTGGCC 
CAAACCAAAA TAGTATTCCC TTTGATTATC CGGTATATAG TAGACATGTT AATCTGGGAA 
GAACAAAAGA GTTTGCCAGC ATATCTGGAT ACAGGGACAG TGGAGTGGAA CCAGTACTAC 
TGTGTACTTT ACAATGATGA ACACCACACC TATGATCACT TCATCTACAG TTTGCAGAGA 
TCGCTTGGTT GTGATCAGAA AGAAGCACAG ACATTAACCA GTGCAGCTGA GAGAGAGGGC 
CGCAAAGCTG TTAAAAAGGG TACTTTAGAG CAATGCCAGG AGGTGACAGA AAGTCTAAAG 
GCTAATTCAG AGAACGTGTC GCTGAAACCT GTCCACGTGG AGGTGCTGCA TGGTCATGTA 
ATGGCCCATC AAAGCTTTGC ACTGTGTTTA GCAATCTGGC TTAACAAACT CCTGGCCTAC 
TCTAGTGACT TCCGACAGAT CTTCTGTCAA GTGTGCCTAG AGCAAGAGCC AAAGCCTAGC 
AGTTTTAATC ACACTTGTTA TGTCAGCTTG TTAATTTTAA AGGACTCCAA GCTTCATAAA 
GGAGTGCGGA AAGTTCTGCA TGATCTGATC TTCAGCAGCT TTTTCATGGA AATGGAGTAT 
AAGAAACAAT TTGCTATAGA GTTTGTTAAG CATTACAAGC AGATACAGAA AGAATACATA 
AACGACGATC AGGACAGGAA CATTTCAGTT ACTGCAATAT CAGTTCAGGT TTTCACTGTC 
CCTACTTTGG CTCGCCTACT GATAGAAGAA CAAAATGTAA TGAAAGTCAT CACAGAGACT 
TTACTCGAGG TCATAAAAGA ATATCTTGGC TCAGACAACA AGTTCAACTT CCAGGGCTAC 
AGCCAGGAAC GCTTTTTCAT GGTATATGCC ATAATCCTTG ACCTCAAGTA TATCCTTATC 
TCTAAACCTA CATCCTGGTC TGAGAAGTTA AAAGCCAAGT TTCTGGAAGG ATTTAAATCT 
TTCCTCTATT TTTTATCCTG TATGCAGGGC ATGGAAGGGA TAAGCAGGAT GGTGGGACAG 
CACGTTGAGA TGGATTCAGA CTGGGAAGCT GTAATAGCTA TGCAGATGCA ACTGAAAAAT 
GTCTTGACGC TGATACAGGA GTGGTGTGCA ACAGATGATG ATATATTGCT CAAATCCTAC 
AAGGAATGCC ATCTGAGTCT TCTGCAGTGT TGCACCCCGG TCCCTTCCCA AGAAAAGAAG 
CTGATAAAAA TGAGTGGCCA TTCATACCAA AGCTATCCAT ACATGGTGTC CAAAGACCCA 
ATCAGCATAC ACCTACCTCT CTCTCGGATG CTTGCTGGTC TACATGCACA GATGTGTAGG 
ACTGGTGTCA TCACAAGACT GTGTGAAATT CTTCCTCCGG AAGACTTACA GGTGGAACTA 
ATGATTGAAC ACCCCTTAAG ATGCCTGGTG CTGGTGGGAC AGGTAGCAGC TGAGATGTGG 
AGAAGGAATG GCCTTACTAT TGTTAGTCAG GTTTTTTACT ATCATGATGT GAAGTGCAGG 
GAGGAGATGT ATGACAAAGA TGTTGTGATG CTACAGATTG GTGCATCCTA CCTGGATCCC 
AACAGCTTCC TACTGCTCTT GCTGCAGAGG TATGAACTGG AGGAGGCATT CTCTAATGTA 
GCTGTGCCCA CCAAAGACAT GGATGTAGCA AGGCAACACA ACATCTTAAT TGAAGAAATG 
TTACATGTCC TTATTTGTGT TATTGGTGAG CGCTATGTGC CAGGCATTAG CAATGTTACT 
AGAGAAGAGT GCACTATGAG GGAGATCATC CATCTGCTTT GTATTGAACC TATGGCTCAC 
AGTGCAATCG CCAAGGCTTT GCCAGAGACT GAAAACACTG ACATATGCCT GGATAAACTC 
ATCAGTAAAG TTGCCACTTT TAAAAAACCG GGAGTATCAG GACATGGCGT TTATGAACTA 
AAGGCTGAAT ACCTCGATCA ATTTAATGTG TTCTTTTATC ATTACACCAA AACACAACAC 
AGCAAGGCAG AACACACACA GAAGAAAAGG CGAAAACAAG AAAACAGCTC AGAAGCATTA 
GCCCCTCCTG CACCCCCCAA TTTCAGCCCA GCTTTTTGCA ACGTGACCAA GCTTCTGTGC 
TGTGATATTA TGATGCATAT TCTACGAACC ATCTTGAAGA GAGCAGCAGA GGAAGATCCA 
ATTATGTGGA CAGAAGGCAT GATACAAATG GTCCTCCATA TTATAGCCCT GGGGTTATTA 
GAAGAGAAGC AACAGCTACA GAGGGCTGCT GAAGAAGAAG TAACATTTAA CTTCTATCAT 
AAGGCAACAA GGGTTAGGAG TACAGATGCA AATGCACAAA GTGTTGCGAC ACTGCTGGAG 
AAGCTGAAGG GCATTACCCA GCTGGATGCC CAGAAAGACA TGATCATTTG GGTGCGACAA 
GTTTTTGAAA CCGTAAAAGG GCTGAGAGAG ACATCTAGTC ACACAGTTCC CACAGATACA 
GTTTCTGACG TTGCAAAAGC TGAAGAGGGA CAAAGCCCCC AGGAAAAGGA GAAGCGCAAA 
AGAAAAGCTG AGGCGGCCCG GCTCCACCGC CAGAAGATCA TGGCACAGAT GTCAGCTTTA 
CAAAAAAATT TCATTGAAAC AAATAAGCTT CTGTATGACA CCACTGTGGA AGTACAAGAA 
AGCACTAGTC CTGTAGAAGA GAGCCTGTTA TCAGCAGATG AAGGATCGAA GGTTGCCTTG 
GGGACAAAAC GTGGCCCCAC AGTGCCTAGC AAAGATGTTC TAACATGTAT TCTATGTCAA 
GAAGAACAGG AAGTGAAATT GGACAAGCCT ACAATGGTGC TAACTGCCTG CGTCCAAAAG 
TCCACAACAC TGTCCCAAAC TCGTAGCAAA GTGGTCTCAC ACACAGGAGA AACGTTTGAT 
CCACTTTTTA CACACCCAGA GATGTTGTAT GGAACCCATA CAGGCAGCTG TGGCCATGTA 
ATGCATGTTG TGTGCTGGCA GAAGTTCTTT GAGGCAATGC AGAAGAATAC ACGGCAGCGT 
CTTCACGTGG AGCTAATTTT TGATCTTGGA AATGGCGAAT ATCTTTGTCC GCTATGCAAA 
TGCCACTGTA ACACCGTTAT ACCTATAATC CCTCATGTTG CCCAGCAAAT ACAAAGTGTG 
GATGCTGAAG CCATAAATTG TGTTTTCTCA CTTCCTCATT GGTTGGACAT AGTGGCGGCC 
AGAATATCTG GTTATAATTT AAACACCATC AAAGGAACAA AGCCAATCAC TCCTGAATTC 
TGTAAAATTT ATAAAGACCA GAATTATGAG TTCCGTTCCA TCTTGAGCTT TGGCGTGCAG 
GCTCCGCCCC GCTATCCAAC TTGCACAGAA GGAATGCTTG TTCACTTTGC CACTACAGTG 
TACCGCGTGG GCTTAAATGT TCCTCCCAAT GAGTCTGATC CACGTATGCC TGTCATGACA 
TGGAATACTT GTGCATTTAC AATCCAGTGT ATAGAAAGCT GGCTTCAGGA TGAAGAAAAA 
CCCTTGTTTG GATCCCTGCA AAACCGGCAA CTTGCTGGAT TACAAGCCTT GGTGAAATTT 
GCTGAGGCCC AAAGAGTTAC ATGCCCTCAG CCTTTGATAC GGAGACATCT TATCCGGTTA 
TTAGCAGCAT TCCTGCCAAA CATTACCCTT GAAGACACCC CATCCTTTCT GTCTTTGGAT 
GTTTTCCATC TGCTGGTGGG GTCAGTGTTG GCATTCCCAT CATTATATTG TGAAGAAAAT 
GTAGACTTAC ATCCTTCTAC CCTTGTTTCT ACCTACAACA ACCTCTACCT CTTTCACCTG 
CTCACAATGA CGCACATACT GCAAATCATT TTCTCTTTAA CTACAGATAA TGTGCCTTCT 
ACAGAACCTG ACGACTGTGA AGAAGCCCGT ACAGCTGCTA CTCTTTGTCA GGACATTTCA 
CAACTCACCA GCAATTTAAA GATTGATTTT CCGGGGTGGT ATGTTTGGAA ATGTGTGAAA 
GAGGGCATCA TGCCATATCT GCGATGTGCA GCTTTGTTCT TCCATTATTT GCTGGGAGTA 
ATGCCTCCAG AGGAGCTAAA ATCATCAGGC ACTACAGACA ATCAGTTATC AGCTCTTTGC 
AGCTTCCTCT GCTTGCCAAC CAACATTCTT CTTCTTTTCC AAGAGAACTG GGACATTGTG 
AAACTTCTCA TTCAGAGGTT GAGTTCTGAC CAATCCCTGC AGAGTGTCCT GGCTGGTTAC 
ACTACTGCAC TCAGGTATCC AAGGAAACGC AATACACTAA TAGAGCTTCC AGAAGATTAC 
AGTTCCCTTC TCAACCAAGC ATCCCAGTTT AGATGTCCTA AGTCTCAAGA TGCTGAACGG 
AAACACCCGG TTATGTGCCT TCTCTGTGGG GCCATTCTCT GTTCTCAGAA TACATGCTGT 
CAGGAGGTGG TAAATGGAGA AGAGCTGGGA GCTTGTACAG CACATGCCAT GCACTGTGGA 
GCTGGTGTCT GCATCTTCCT AAGTATCAGA GAATGCAAAG TGGTCCTAAT GGAGGGTAAA 
ACCAGAGGAT GTATCTACCA GGCTCCCTAT CTTGATGAAT ATGGAGAAAC AGACCCAGGA 
CTGAAACGTG GAAACCCCTT ACACCTTTCC CATGAGCGAT ATCGGAAATT GCATCAGTTG 
TGGCAGCAGC ACTGTATTAT TGAAGAGATT GGACACAACC TTGAAATCAA CCAGATGTTT 
TTTGACTTTA ATTGGAGCCT GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ubr1 ( XM_012969238.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012824692.1 Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X3, mRNA.

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CloneID	OXa34673
Clone ID Related Accession (Same CDS sequence)	XM_012969238.2
Accession Version	XM_012969238.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969238.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTT TCCAGAACAG CATTCATAAA AATCACAGAT ACAAGATGCA CTCTTCAATG 
GGAGGGGGAT TTTGTGACTG TGGGGATACT GAAGCCTGGA AAACCGGCCC ATATTGTAAG 
ATCCATGAAC CAGGGGCATC AGATCAGAAT TCAGACTTTC GTTTGAAGGA TGAACTTCTG 
GCCCAAACCA AAATAGTATT CCCTTTGATT ATCCGGTATA TAGTAGACAT GTTAATCTGG 
GAAGAACAAA AGAGTTTGCC AGCATATCTG GATACAGGGA CAGTGGAGTG GAACCAGTAC 
TACTGTGTAC TTTACAATGA TGAACACCAC ACCTATGATC ACTTCATCTA CAGTTTGCAG 
AGATCGCTTG GTTGTGATCA GAAAGAAGCA CAGACATTAA CCAGTGCAGC TGAGAGAGAG 
GGCCGCAAAG CTGTTAAAAA GGGTACTTTA GAGCAATGCC AGGAGGTGAC AGAAAGTCTA 
AAGGCTAATT CAGAGAACGT GTCGCTGAAA CCTGTCCACG TGGAGGTGCT GCATGGTCAT 
GTAATGGCCC ATCAAAGCTT TGCACTGTGT TTAGCAATCT GGCTTAACAA ACTCCTGGCC 
TACTCTAGTG ACTTCCGACA GATCTTCTGT CAAGTGTGCC TAGAGCAAGA GCCAAAGCCT 
AGCAGTTTTA ATCACACTTG TTATGTCAGC TTGTTAATTT TAAAGGACTC CAAGCTTCAT 
AAAGGAGTGC GGAAAGTTCT GCATGATCTG ATCTTCAGCA GCTTTTTCAT GGAAATGGAG 
TATAAGAAAC AATTTGCTAT AGAGTTTGTT AAGCATTACA AGCAGATACA GAAAGAATAC 
ATAAACGACG ATCAGGACAG GAACATTTCA GTTACTGCAA TATCAGTTCA GGTTTTCACT 
GTCCCTACTT TGGCTCGCCT ACTGATAGAA GAACAAAATG TAATGAAAGT CATCACAGAG 
ACTTTACTCG AGGTCATAAA AGAATATCTT GGCTCAGACA ACAAGTTCAA CTTCCAGGGC 
TACAGCCAGG AACGCTTTTT CATGGTATAT GCCATAATCC TTGACCTCAA GTATATCCTT 
ATCTCTAAAC CTACATCCTG GTCTGAGAAG TTAAAAGCCA AGTTTCTGGA AGGATTTAAA 
TCTTTCCTCT ATTTTTTATC CTGTATGCAG GGCATGGAAG GGATAAGCAG GATGGTGGGA 
CAGCACGTTG AGATGGATTC AGACTGGGAA GCTGTAATAG CTATGCAGAT GCAACTGAAA 
AATGTCTTGA CGCTGATACA GGAGTGGTGT GCAACAGATG ATGATATATT GCTCAAATCC 
TACAAGGAAT GCCATCTGAG TCTTCTGCAG TGTTGCACCC CGGTCCCTTC CCAAGAAAAG 
AAGCTGATAA AAATGAGTGG CCATTCATAC CAAAGCTATC CATACATGGT GTCCAAAGAC 
CCAATCAGCA TACACCTACC TCTCTCTCGG ATGCTTGCTG GTCTACATGC ACAGATGTGT 
AGGACTGGTG TCATCACAAG ACTGTGTGAA ATTCTTCCTC CGGAAGACTT ACAGGTGGAA 
CTAATGATTG AACACCCCTT AAGATGCCTG GTGCTGGTGG GACAGGTAGC AGCTGAGATG 
TGGAGAAGGA ATGGCCTTAC TATTGTTAGT CAGGTTTTTT ACTATCATGA TGTGAAGTGC 
AGGGAGGAGA TGTATGACAA AGATGTTGTG ATGCTACAGA TTGGTGCATC CTACCTGGAT 
CCCAACAGCT TCCTACTGCT CTTGCTGCAG AGGTATGAAC TGGAGGAGGC ATTCTCTAAT 
GTAGCTGTGC CCACCAAAGA CATGGATGTA GCAAGGCAAC ACAACATCTT AATTGAAGAA 
ATGTTACATG TCCTTATTTG TGTTATTGGT GAGCGCTATG TGCCAGGCAT TAGCAATGTT 
ACTAGAGAAG AGTGCACTAT GAGGGAGATC ATCCATCTGC TTTGTATTGA ACCTATGGCT 
CACAGTGCAA TCGCCAAGGC TTTGCCAGAG ACTGAAAACA CTGACATATG CCTGGATAAA 
CTCATCAGTA AAGTTGCCAC TTTTAAAAAA CCGGGAGTAT CAGGACATGG CGTTTATGAA 
CTAAAGGCTG AATACCTCGA TCAATTTAAT GTGTTCTTTT ATCATTACAC CAAAACACAA 
CACAGCAAGG CAGAACACAC ACAGAAGAAA AGGCGAAAAC AAGAAAACAG CTCAGAAGCA 
TTAGCCCCTC CTGCACCCCC CAATTTCAGC CCAGCTTTTT GCAACGTGAC CAAGCTTCTG 
TGCTGTGATA TTATGATGCA TATTCTACGA ACCATCTTGA AGAGAGCAGC AGAGGAAGAT 
CCAATTATGT GGACAGAAGG CATGATACAA ATGGTCCTCC ATATTATAGC CCTGGGGTTA 
TTAGAAGAGA AGCAACAGCT ACAGAGGGCT GCTGAAGAAG AAGTAACATT TAACTTCTAT 
CATAAGGCAA CAAGGGTTAG GAGTACAGAT GCAAATGCAC AAAGTGTTGC GACACTGCTG 
GAGAAGCTGA AGGGCATTAC CCAGCTGGAT GCCCAGAAAG ACATGATCAT TTGGGTGCGA 
CAAGTTTTTG AAACCGTAAA AGGGCTGAGA GAGACATCTA GTCACACAGT TCCCACAGAT 
ACAGTTTCTG ACGTTGCAAA AGCTGAAGAG GGACAAAGCC CCCAGGAAAA GGAGAAGCGC 
AAAAGAAAAG CTGAGGCGGC CCGGCTCCAC CGCCAGAAGA TCATGGCACA GATGTCAGCT 
TTACAAAAAA ATTTCATTGA AACAAATAAG CTTCTGTATG ACACCACTGT GGAAGTACAA 
GAAAGCACTA GTCCTGTAGA AGAGAGCCTG TTATCAGCAG ATGAAGGATC GAAGGTTGCC 
TTGGGGACAA AACGTGGCCC CACAGTGCCT AGCAAAGATG TTCTAACATG TATTCTATGT 
CAAGAAGAAC AGGAAGTGAA ATTGGACAAG CCTACAATGG TGCTAACTGC CTGCGTCCAA 
AAGTCCACAA CACTGTCCCA AACTCGTAGC AAAGTGGTCT CACACACAGG AGAAACGTTT 
GATCCACTTT TTACACACCC AGAGATGTTG TATGGAACCC ATACAGGCAG CTGTGGCCAT 
GTAATGCATG TTGTGTGCTG GCAGAAGTTC TTTGAGGCAA TGCAGAAGAA TACACGGCAG 
CGTCTTCACG TGGAGCTAAT TTTTGATCTT GGAAATGGCG AATATCTTTG TCCGCTATGC 
AAATGCCACT GTAACACCGT TATACCTATA ATCCCTCATG TTGCCCAGCA AATACAAAGT 
GTGGATGCTG AAGCCATAAA TTGTGTTTTC TCACTTCCTC ATTGGTTGGA CATAGTGGCG 
GCCAGAATAT CTGGTTATAA TTTAAACACC ATCAAAGGAA CAAAGCCAAT CACTCCTGAA 
TTCTGTAAAA TTTATAAAGA CCAGAATTAT GAGTTCCGTT CCATCTTGAG CTTTGGCGTG 
CAGGCTCCGC CCCGCTATCC AACTTGCACA GAAGGAATGC TTGTTCACTT TGCCACTACA 
GTGTACCGCG TGGGCTTAAA TGTTCCTCCC AATGAGTCTG ATCCACGTAT GCCTGTCATG 
ACATGGAATA CTTGTGCATT TACAATCCAG TGTATAGAAA GCTGGCTTCA GGATGAAGAA 
AAACCCTTGT TTGGATCCCT GCAAAACCGG CAACTTGCTG GATTACAAGC CTTGGTGAAA 
TTTGCTGAGG CCCAAAGAGT TACATGCCCT CAGCCTTTGA TACGGAGACA TCTTATCCGG 
TTATTAGCAG CATTCCTGCC AAACATTACC CTTGAAGACA CCCCATCCTT TCTGTCTTTG 
GATGTTTTCC ATCTGCTGGT GGGGTCAGTG TTGGCATTCC CATCATTATA TTGTGAAGAA 
AATGTAGACT TACATCCTTC TACCCTTGTT TCTACCTACA ACAACCTCTA CCTCTTTCAC 
CTGCTCACAA TGACGCACAT ACTGCAAATC ATTTTCTCTT TAACTACAGA TAATGTGCCT 
TCTACAGAAC CTGACGACTG TGAAGAAGCC CGTACAGCTG CTACTCTTTG TCAGGACATT 
TCACAACTCA CCAGCAACAG TTTAAAGATT GATTTTCCGG GGTGGTATGT TTGGAAATGT 
GTGAAAGAGG GCATCATGCC ATATCTGCGA TGTGCAGCTT TGTTCTTCCA TTATTTGCTG 
GGAGTAATGC CTCCAGAGGA GCTAAAATCA TCAGGCACTA CAGACAATCA GTTATCAGCT 
CTTTGCAGCT TCCTCTGCTT GCCAACCAAC ATTCTTCTTC TTTTCCAAGA GAACTGGGAC 
ATTGTGAAAC TTCTCATTCA GAGGTTGAGT TCTGACCAAT CCCTGCAGAG TGTCCTGGCT 
GGTTACACTA CTGCACTCAG GTATCCAAGG AAACGCAATA CACTAATAGA GCTTCCAGAA 
GATTACAGTT CCCTTCTCAA CCAAGCATCC CAGTTTAGAT GTCCTAAGTC TCAAGATGCT 
GAACGGAAAC ACCCGGTTAT GTGCCTTCTC TGTGGGGCCA TTCTCTGTTC TCAGAATACA 
TGCTGTCAGG AGGTGGTAAA TGGAGAAGAG CTGGGAGCTT GTACAGCACA TGCCATGCAC 
TGTGGAGCTG GTGTCTGCAT CTTCCTAAGT ATCAGAGAAT GCAAAGTGGT CCTAATGGAG 
GGTAAAACCA GAGGATGTAT CTACCAGGCT CCCTATCTTG ATGAATATGG AGAAACAGAC 
CCAGGACTGA AACGTGGAAA CCCCTTACAC CTTTCCCATG AGCGATATCG GAAATTGCAT 
CAGTTGTGGC AGCAGCACTG TATTATTGAA GAGATTGGAC ACAACCTTGA AATCAACCAG 
ATGTTTTTTG ACTTTAATTG GAGCCTGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012824692.1
CDS	383..5275
Translation	MDCFQNSIHKNHRYKMHSSMGGGFCDCGDTEAWKTGPYCKIHEPGASDQNSDFRLKDELLAQTKIVFPLIIRYIVDMLIWEEQKSLPAYLDTGTVEWNQYYCVLYNDEHHTYDHFIYSLQRSLGCDQKEAQTLTSAAEREGRKAVKKGTLEQCQEVTESLKANSENVSLKPVHVEVLHGHVMAHQSFALCLAIWLNKLLAYSSDFRQIFCQVCLEQEPKPSSFNHTCYVSLLILKDSKLHKGVRKVLHDLIFSSFFMEMEYKKQFAIEFVKHYKQIQKEYINDDQDRNISVTAISVQVFTVPTLARLLIEEQNVMKVITETLLEVIKEYLGSDNKFNFQGYSQERFFMVYAIILDLKYILISKPTSWSEKLKAKFLEGFKSFLYFLSCMQGMEGISRMVGQHVEMDSDWEAVIAMQMQLKNVLTLIQEWCATDDDILLKSYKECHLSLLQCCTPVPSQEKKLIKMSGHSYQSYPYMVSKDPISIHLPLSRMLAGLHAQMCRTGVITRLCEILPPEDLQVELMIEHPLRCLVLVGQVAAEMWRRNGLTIVSQVFYYHDVKCREEMYDKDVVMLQIGASYLDPNSFLLLLLQRYELEEAFSNVAVPTKDMDVARQHNILIEEMLHVLICVIGERYVPGISNVTREECTMREIIHLLCIEPMAHSAIAKALPETENTDICLDKLISKVATFKKPGVSGHGVYELKAEYLDQFNVFFYHYTKTQHSKAEHTQKKRRKQENSSEALAPPAPPNFSPAFCNVTKLLCCDIMMHILRTILKRAAEEDPIMWTEGMIQMVLHIIALGLLEEKQQLQRAAEEEVTFNFYHKATRVRSTDANAQSVATLLEKLKGITQLDAQKDMIIWVRQVFETVKGLRETSSHTVPTDTVSDVAKAEEGQSPQEKEKRKRKAEAARLHRQKIMAQMSALQKNFIETNKLLYDTTVEVQESTSPVEESLLSADEGSKVALGTKRGPTVPSKDVLTCILCQEEQEVKLDKPTMVLTACVQKSTTLSQTRSKVVSHTGETFDPLFTHPEMLYGTHTGSCGHVMHVVCWQKFFEAMQKNTRQRLHVELIFDLGNGEYLCPLCKCHCNTVIPIIPHVAQQIQSVDAEAINCVFSLPHWLDIVAARISGYNLNTIKGTKPITPEFCKIYKDQNYEFRSILSFGVQAPPRYPTCTEGMLVHFATTVYRVGLNVPPNESDPRMPVMTWNTCAFTIQCIESWLQDEEKPLFGSLQNRQLAGLQALVKFAEAQRVTCPQPLIRRHLIRLLAAFLPNITLEDTPSFLSLDVFHLLVGSVLAFPSLYCEENVDLHPSTLVSTYNNLYLFHLLTMTHILQIIFSLTTDNVPSTEPDDCEEARTAATLCQDISQLTSNSLKIDFPGWYVWKCVKEGIMPYLRCAALFFHYLLGVMPPEELKSSGTTDNQLSALCSFLCLPTNILLLFQENWDIVKLLIQRLSSDQSLQSVLAGYTTALRYPRKRNTLIELPEDYSSLLNQASQFRCPKSQDAERKHPVMCLLCGAILCSQNTCCQEVVNGEELGACTAHAMHCGAGVCIFLSIRECKVVLMEGKTRGCIYQAPYLDEYGETDPGLKRGNPLHLSHERYRKLHQLWQQHCIIEEIGHNLEINQMFFDFNWSLL

Target ORF information:

RefSeq Version	XM_012969238.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin protein ligase E3 component n-recognin 1 (ubr1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012969238.2

ORF Insert Sequence:

ATGGATTGTT TCCAGAACAG CATTCATAAA AATCACAGAT ACAAGATGCA CTCTTCAATG 
GGAGGGGGAT TTTGTGACTG TGGGGATACT GAAGCCTGGA AAACCGGCCC ATATTGTAAG 
ATCCATGAAC CAGGGGCATC AGATCAGAAT TCAGACTTTC GTTTGAAGGA TGAACTTCTG 
GCCCAAACCA AAATAGTATT CCCTTTGATT ATCCGGTATA TAGTAGACAT GTTAATCTGG 
GAAGAACAAA AGAGTTTGCC AGCATATCTG GATACAGGGA CAGTGGAGTG GAACCAGTAC 
TACTGTGTAC TTTACAATGA TGAACACCAC ACCTATGATC ACTTCATCTA CAGTTTGCAG 
AGATCGCTTG GTTGTGATCA GAAAGAAGCA CAGACATTAA CCAGTGCAGC TGAGAGAGAG 
GGCCGCAAAG CTGTTAAAAA GGGTACTTTA GAGCAATGCC AGGAGGTGAC AGAAAGTCTA 
AAGGCTAATT CAGAGAACGT GTCGCTGAAA CCTGTCCACG TGGAGGTGCT GCATGGTCAT 
GTAATGGCCC ATCAAAGCTT TGCACTGTGT TTAGCAATCT GGCTTAACAA ACTCCTGGCC 
TACTCTAGTG ACTTCCGACA GATCTTCTGT CAAGTGTGCC TAGAGCAAGA GCCAAAGCCT 
AGCAGTTTTA ATCACACTTG TTATGTCAGC TTGTTAATTT TAAAGGACTC CAAGCTTCAT 
AAAGGAGTGC GGAAAGTTCT GCATGATCTG ATCTTCAGCA GCTTTTTCAT GGAAATGGAG 
TATAAGAAAC AATTTGCTAT AGAGTTTGTT AAGCATTACA AGCAGATACA GAAAGAATAC 
ATAAACGACG ATCAGGACAG GAACATTTCA GTTACTGCAA TATCAGTTCA GGTTTTCACT 
GTCCCTACTT TGGCTCGCCT ACTGATAGAA GAACAAAATG TAATGAAAGT CATCACAGAG 
ACTTTACTCG AGGTCATAAA AGAATATCTT GGCTCAGACA ACAAGTTCAA CTTCCAGGGC 
TACAGCCAGG AACGCTTTTT CATGGTATAT GCCATAATCC TTGACCTCAA GTATATCCTT 
ATCTCTAAAC CTACATCCTG GTCTGAGAAG TTAAAAGCCA AGTTTCTGGA AGGATTTAAA 
TCTTTCCTCT ATTTTTTATC CTGTATGCAG GGCATGGAAG GGATAAGCAG GATGGTGGGA 
CAGCACGTTG AGATGGATTC AGACTGGGAA GCTGTAATAG CTATGCAGAT GCAACTGAAA 
AATGTCTTGA CGCTGATACA GGAGTGGTGT GCAACAGATG ATGATATATT GCTCAAATCC 
TACAAGGAAT GCCATCTGAG TCTTCTGCAG TGTTGCACCC CGGTCCCTTC CCAAGAAAAG 
AAGCTGATAA AAATGAGTGG CCATTCATAC CAAAGCTATC CATACATGGT GTCCAAAGAC 
CCAATCAGCA TACACCTACC TCTCTCTCGG ATGCTTGCTG GTCTACATGC ACAGATGTGT 
AGGACTGGTG TCATCACAAG ACTGTGTGAA ATTCTTCCTC CGGAAGACTT ACAGGTGGAA 
CTAATGATTG AACACCCCTT AAGATGCCTG GTGCTGGTGG GACAGGTAGC AGCTGAGATG 
TGGAGAAGGA ATGGCCTTAC TATTGTTAGT CAGGTTTTTT ACTATCATGA TGTGAAGTGC 
AGGGAGGAGA TGTATGACAA AGATGTTGTG ATGCTACAGA TTGGTGCATC CTACCTGGAT 
CCCAACAGCT TCCTACTGCT CTTGCTGCAG AGGTATGAAC TGGAGGAGGC ATTCTCTAAT 
GTAGCTGTGC CCACCAAAGA CATGGATGTA GCAAGGCAAC ACAACATCTT AATTGAAGAA 
ATGTTACATG TCCTTATTTG TGTTATTGGT GAGCGCTATG TGCCAGGCAT TAGCAATGTT 
ACTAGAGAAG AGTGCACTAT GAGGGAGATC ATCCATCTGC TTTGTATTGA ACCTATGGCT 
CACAGTGCAA TCGCCAAGGC TTTGCCAGAG ACTGAAAACA CTGACATATG CCTGGATAAA 
CTCATCAGTA AAGTTGCCAC TTTTAAAAAA CCGGGAGTAT CAGGACATGG CGTTTATGAA 
CTAAAGGCTG AATACCTCGA TCAATTTAAT GTGTTCTTTT ATCATTACAC CAAAACACAA 
CACAGCAAGG CAGAACACAC ACAGAAGAAA AGGCGAAAAC AAGAAAACAG CTCAGAAGCA 
TTAGCCCCTC CTGCACCCCC CAATTTCAGC CCAGCTTTTT GCAACGTGAC CAAGCTTCTG 
TGCTGTGATA TTATGATGCA TATTCTACGA ACCATCTTGA AGAGAGCAGC AGAGGAAGAT 
CCAATTATGT GGACAGAAGG CATGATACAA ATGGTCCTCC ATATTATAGC CCTGGGGTTA 
TTAGAAGAGA AGCAACAGCT ACAGAGGGCT GCTGAAGAAG AAGTAACATT TAACTTCTAT 
CATAAGGCAA CAAGGGTTAG GAGTACAGAT GCAAATGCAC AAAGTGTTGC GACACTGCTG 
GAGAAGCTGA AGGGCATTAC CCAGCTGGAT GCCCAGAAAG ACATGATCAT TTGGGTGCGA 
CAAGTTTTTG AAACCGTAAA AGGGCTGAGA GAGACATCTA GTCACACAGT TCCCACAGAT 
ACAGTTTCTG ACGTTGCAAA AGCTGAAGAG GGACAAAGCC CCCAGGAAAA GGAGAAGCGC 
AAAAGAAAAG CTGAGGCGGC CCGGCTCCAC CGCCAGAAGA TCATGGCACA GATGTCAGCT 
TTACAAAAAA ATTTCATTGA AACAAATAAG CTTCTGTATG ACACCACTGT GGAAGTACAA 
GAAAGCACTA GTCCTGTAGA AGAGAGCCTG TTATCAGCAG ATGAAGGATC GAAGGTTGCC 
TTGGGGACAA AACGTGGCCC CACAGTGCCT AGCAAAGATG TTCTAACATG TATTCTATGT 
CAAGAAGAAC AGGAAGTGAA ATTGGACAAG CCTACAATGG TGCTAACTGC CTGCGTCCAA 
AAGTCCACAA CACTGTCCCA AACTCGTAGC AAAGTGGTCT CACACACAGG AGAAACGTTT 
GATCCACTTT TTACACACCC AGAGATGTTG TATGGAACCC ATACAGGCAG CTGTGGCCAT 
GTAATGCATG TTGTGTGCTG GCAGAAGTTC TTTGAGGCAA TGCAGAAGAA TACACGGCAG 
CGTCTTCACG TGGAGCTAAT TTTTGATCTT GGAAATGGCG AATATCTTTG TCCGCTATGC 
AAATGCCACT GTAACACCGT TATACCTATA ATCCCTCATG TTGCCCAGCA AATACAAAGT 
GTGGATGCTG AAGCCATAAA TTGTGTTTTC TCACTTCCTC ATTGGTTGGA CATAGTGGCG 
GCCAGAATAT CTGGTTATAA TTTAAACACC ATCAAAGGAA CAAAGCCAAT CACTCCTGAA 
TTCTGTAAAA TTTATAAAGA CCAGAATTAT GAGTTCCGTT CCATCTTGAG CTTTGGCGTG 
CAGGCTCCGC CCCGCTATCC AACTTGCACA GAAGGAATGC TTGTTCACTT TGCCACTACA 
GTGTACCGCG TGGGCTTAAA TGTTCCTCCC AATGAGTCTG ATCCACGTAT GCCTGTCATG 
ACATGGAATA CTTGTGCATT TACAATCCAG TGTATAGAAA GCTGGCTTCA GGATGAAGAA 
AAACCCTTGT TTGGATCCCT GCAAAACCGG CAACTTGCTG GATTACAAGC CTTGGTGAAA 
TTTGCTGAGG CCCAAAGAGT TACATGCCCT CAGCCTTTGA TACGGAGACA TCTTATCCGG 
TTATTAGCAG CATTCCTGCC AAACATTACC CTTGAAGACA CCCCATCCTT TCTGTCTTTG 
GATGTTTTCC ATCTGCTGGT GGGGTCAGTG TTGGCATTCC CATCATTATA TTGTGAAGAA 
AATGTAGACT TACATCCTTC TACCCTTGTT TCTACCTACA ACAACCTCTA CCTCTTTCAC 
CTGCTCACAA TGACGCACAT ACTGCAAATC ATTTTCTCTT TAACTACAGA TAATGTGCCT 
TCTACAGAAC CTGACGACTG TGAAGAAGCC CGTACAGCTG CTACTCTTTG TCAGGACATT 
TCACAACTCA CCAGCAACAG TTTAAAGATT GATTTTCCGG GGTGGTATGT TTGGAAATGT 
GTGAAAGAGG GCATCATGCC ATATCTGCGA TGTGCAGCTT TGTTCTTCCA TTATTTGCTG 
GGAGTAATGC CTCCAGAGGA GCTAAAATCA TCAGGCACTA CAGACAATCA GTTATCAGCT 
CTTTGCAGCT TCCTCTGCTT GCCAACCAAC ATTCTTCTTC TTTTCCAAGA GAACTGGGAC 
ATTGTGAAAC TTCTCATTCA GAGGTTGAGT TCTGACCAAT CCCTGCAGAG TGTCCTGGCT 
GGTTACACTA CTGCACTCAG GTATCCAAGG AAACGCAATA CACTAATAGA GCTTCCAGAA 
GATTACAGTT CCCTTCTCAA CCAAGCATCC CAGTTTAGAT GTCCTAAGTC TCAAGATGCT 
GAACGGAAAC ACCCGGTTAT GTGCCTTCTC TGTGGGGCCA TTCTCTGTTC TCAGAATACA 
TGCTGTCAGG AGGTGGTAAA TGGAGAAGAG CTGGGAGCTT GTACAGCACA TGCCATGCAC 
TGTGGAGCTG GTGTCTGCAT CTTCCTAAGT ATCAGAGAAT GCAAAGTGGT CCTAATGGAG 
GGTAAAACCA GAGGATGTAT CTACCAGGCT CCCTATCTTG ATGAATATGG AGAAACAGAC 
CCAGGACTGA AACGTGGAAA CCCCTTACAC CTTTCCCATG AGCGATATCG GAAATTGCAT 
CAGTTGTGGC AGCAGCACTG TATTATTGAA GAGATTGGAC ACAACCTTGA AATCAACCAG 
ATGTTTTTTG ACTTTAATTG GAGCCTGCTT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ubr1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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