pde6a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pde6a
Entrez Gene ID	100489913
Full Name	phosphodiesterase 6A
General protein information	Preferred Names phosphodiesterase 6A Names rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha phosphodiesterase 6A, cGMP-specific, rod, alpha
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002936704.3	XP_002936750.2	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
XM_002936704.4	XP_002936750.3	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDE6A	NP_000431.2
Pan troglodytes (chimpanzee)	PDE6A	XP_518030.2
Mus musculus (house mouse)	Pde6a	NP_666198.1
Rattus norvegicus (Norway rat)	Pde6a	NP_001100856.1
Xenopus tropicalis (tropical clawed frog)	pde6a	XP_002936750.2
Canis lupus familiaris (dog)	PDE6A	NP_001003073.1
Danio rerio (zebrafish)	pde6a	NP_001007161.1
Bos taurus (cattle)	PDE6A	NP_001001526.2
	PDE6A	XP_001107349.1

Gene Ontology

The following pde6a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pde6a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa07613	XM_002936704.3 Latest version!	Xenopus tropicalis phosphodiesterase 6A (pde6a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa54526	XM_002936704.4 Latest version!	Xenopus tropicalis phosphodiesterase 6A (pde6a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pde6a ( XM_002936704.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936750.2 Xenopus tropicalis phosphodiesterase 6A (pde6a), mRNA.

Price & Availability↑

CloneID	OXa07613
Clone ID Related Accession (Same CDS sequence)	XM_002936704.3
Accession Version	XM_002936704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002936704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCTA TAACGCAGGC GGAAGTGGAG AAATTTCTGG ATTCCAACCC ATCTTTTGCC 
AAACAGTACT ACGACCTTAA ATTTAGACCC GGTATAATCT GTGATCTGCT CAGCACTGAA 
AGGACCAATG TGGACTTCTC CCACGTTCAT ACTTTGAGCA GTGTGGAGGA GAGCGAAATT 
GTCTTTGACC TCATCAGAGG ATTCCAGGAG AACCTCAATC TTGAAAGATG TGTTTTCAGT 
GTGCTCAAGA TGATGTCTTT CATAATTCAG GCAGATCGCA TGAGTTTGTT CATGTACCGC 
ATGAGGAATG GCACCGCTGA GCTTGCCACC AGGCTTTTTA ATGTTAATAA AGATGCCACC 
TTGGACGAAT GTGTGGTGGT TCCAGACTCT GAGATTGTGT TTCCTCTGGA TACTGGAGTA 
TTGGGCTTTG TGGCACAGTC TAAGAAATTA GTGAATGTTT CAGATGTGAC ACAGGATTCT 
CATTTCTGCA GCTTTGTAGA TAACCTTACA GAGTATGACA CCAAAAGCAT CCTGGCTGCT 
CCTGTCCTGA ATGGAAAGGA TGTTGTCGCT GTAATAATGG CTGTCAACAA ATCAGATGGC 
ACCAATTTCA CCAAAACTGA TGAAGAGATG TTCCTTAAGT ACCTGAGTTT TGCCAATCTG 
GTTCTCAAGG TTTATCACCT TTCCTACCTT CACAACTGTG AGACTAGAAG AGGACAGATA 
CTTCTGTGGT CAGCCAATAA GGTTTTTGAA GAACTTACTG ACATAGAGAG ACAGTTTCAC 
AAAGCGCTTT ACACAGTCCG CGCCTTCCTT AACTGTGACA GATACTCTGT GGGACTGCTG 
GATATGACTA AAACTAAGGA ATTTTTTGAC CAATGGCCAA TACTCATGGG AGAAGTCCCC 
CCATATTCAG GACCCAAGAC TCCTGATGGG AGGGAAATAT CATTCTACAA AGTGATTGAC 
TATATTTTAC ATGGAAAAGA AGAAATTAAA GTTATACCGA ACCCCCCAGC AGATCACTGG 
TCTCTGGTTA GCAAACTACC AAGTTACGTT GCTGAAAACG GTCTTATTTG CAATATCATG 
AACACAAGTG CTGATGAATA CTTTGATTTT CAGAAAGGTC CGGTTGACGA CTCTGGGTGG 
ATTATGAAAA ATGTCCTTTC CATGCCAATC GTCAACAAGA AAGAAGAAAT TCTTGGCGTT 
GCAACGTTTT ACAACAGAAA GGATGGAAAA CCTTTTGATG AAATGGACGA AGTTTTGACA 
GAATCCTTAA CACAGTTCCT GGGATGGTCT GTCCTAAATA CTGATACGTA TGAGAAAATG 
AACAAACTCG AGAACAGAAA GGATATTTAC CAAGACATGG TCAACTATCA TGTGAAATGT 
TCAAACGATG AACTGCAGAT TATATTGAAA ACTAAGGAAA TCTATAACAG GCTGCCAGAA 
GAGTGCGAAG AAGAAGAATT ATATGAAATC TTGCAAGAAG AGCTTCCTGA CCCAACAGAA 
GCCGAAATTT ATGAATTCCA CTTCAGCGAC TTACCAGTCT CTGAACTTTT CTTGGTCAAA 
TGCGCAATTC AAATATATTA TGAGCTGGAA GTGGTAGACA AGTTCCATAT ACCACAAGAG 
GTATTAGTCC GCTTTTTATT TTCTGTAAGC AAAGGATACC GGAAAATAAC CTATCACAAC 
TGGCGGCATG GCTTTAATGT CGGACAGACA ATGTTTACAC TTCTCATGAC TGGAAACCTA 
AAGAGGTATT ATACAGATCT GGAGGCCATG GCTATGGTAA CAGCAGCTTT CTGCCATGAC 
ATTGACCACA GAGGAACTAA TAACTTGTAC CAGATGAAGT CTGGGCATCC ATTAGCAAAG 
CTCCATGGAT CCTCAATTCT AGAAAGGCAT CATTTGGAAT TTGGCAAATT TTTACTGAAA 
GATGAGTCAT TGAATATCTT CCAAAACCTC AACAGGAGGC AGTACGACCA TGCAATCCAC 
ATGATGGATA TTGCAATCAT TGCAACTGAT TTAGCCTTGT ATTTTAAAAA GAGGGCAATG 
TTTCAGAAGA TAGTTGACCA GTCTCAAACA TTCACAAGTG AAAAAGATTG GACAGAATAT 
ATGATGCTGG AACAGACCCG TAAAGAAATT GTCATGGCAA TGATGATGAC AGCTTGTGAT 
CTCTCAGCCA TAGCCAAGCC CTGGGATATA CAAAGCAAGG TTGCGTTGTT GGTTGCTGCA 
GAATTCTGGG AACAAGGTGA TTTGGAAAGG ACTGTATTGC AACAAAACCC AATCCCAATG 
ATGGACAGGA ACAAAGCAGA TGAACTTCCT AAGCTTCAGT GTGGTTTCAT TGACTTTGTG 
TGCACGTTTG TATATAAGGA ATTTTCTAGG TTCCATCCTG AGATACAGCC AATGTATGAT 
GGGATTCTAA ATAATAAGAA GGAGTGGAAA AAATTGGCTG ATGAATATGA TGCAAAAATG 
AAAACCCTGG AAGAGGACAA GCAAAAAAAG GAGGGGATAC AGCCACCAAA ACAAGTTCCA 
CAACCTACAG TGAGAGAAAA TGGAGGAAAC GTCCCAGTTA CCGAAGCAGC ACCACAGTCC 
AAAAGTTGTT CTATCTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936750.2
CDS	140..2740
Translation	MSSITQAEVEKFLDSNPSFAKQYYDLKFRPGIICDLLSTERTNVDFSHVHTLSSVEESEIVFDLIRGFQENLNLERCVFSVLKMMSFIIQADRMSLFMYRMRNGTAELATRLFNVNKDATLDECVVVPDSEIVFPLDTGVLGFVAQSKKLVNVSDVTQDSHFCSFVDNLTEYDTKSILAAPVLNGKDVVAVIMAVNKSDGTNFTKTDEEMFLKYLSFANLVLKVYHLSYLHNCETRRGQILLWSANKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLLDMTKTKEFFDQWPILMGEVPPYSGPKTPDGREISFYKVIDYILHGKEEIKVIPNPPADHWSLVSKLPSYVAENGLICNIMNTSADEYFDFQKGPVDDSGWIMKNVLSMPIVNKKEEILGVATFYNRKDGKPFDEMDEVLTESLTQFLGWSVLNTDTYEKMNKLENRKDIYQDMVNYHVKCSNDELQIILKTKEIYNRLPEECEEEELYEILQEELPDPTEAEIYEFHFSDLPVSELFLVKCAIQIYYELEVVDKFHIPQEVLVRFLFSVSKGYRKITYHNWRHGFNVGQTMFTLLMTGNLKRYYTDLEAMAMVTAAFCHDIDHRGTNNLYQMKSGHPLAKLHGSSILERHHLEFGKFLLKDESLNIFQNLNRRQYDHAIHMMDIAIIATDLALYFKKRAMFQKIVDQSQTFTSEKDWTEYMMLEQTRKEIVMAMMMTACDLSAIAKPWDIQSKVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELPKLQCGFIDFVCTFVYKEFSRFHPEIQPMYDGILNNKKEWKKLADEYDAKMKTLEEDKQKKEGIQPPKQVPQPTVRENGGNVPVTEAAPQSKSCSIL

Target ORF information:

RefSeq Version	XM_002936704.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 6A (pde6a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936704.3

ORF Insert Sequence:

ATGAGTTCTA TAACGCAGGC GGAAGTGGAG AAATTTCTGG ATTCCAACCC ATCTTTTGCC 
AAACAGTACT ACGACCTTAA ATTTAGACCC GGTATAATCT GTGATCTGCT CAGCACTGAA 
AGGACCAATG TGGACTTCTC CCACGTTCAT ACTTTGAGCA GTGTGGAGGA GAGCGAAATT 
GTCTTTGACC TCATCAGAGG ATTCCAGGAG AACCTCAATC TTGAAAGATG TGTTTTCAGT 
GTGCTCAAGA TGATGTCTTT CATAATTCAG GCAGATCGCA TGAGTTTGTT CATGTACCGC 
ATGAGGAATG GCACCGCTGA GCTTGCCACC AGGCTTTTTA ATGTTAATAA AGATGCCACC 
TTGGACGAAT GTGTGGTGGT TCCAGACTCT GAGATTGTGT TTCCTCTGGA TACTGGAGTA 
TTGGGCTTTG TGGCACAGTC TAAGAAATTA GTGAATGTTT CAGATGTGAC ACAGGATTCT 
CATTTCTGCA GCTTTGTAGA TAACCTTACA GAGTATGACA CCAAAAGCAT CCTGGCTGCT 
CCTGTCCTGA ATGGAAAGGA TGTTGTCGCT GTAATAATGG CTGTCAACAA ATCAGATGGC 
ACCAATTTCA CCAAAACTGA TGAAGAGATG TTCCTTAAGT ACCTGAGTTT TGCCAATCTG 
GTTCTCAAGG TTTATCACCT TTCCTACCTT CACAACTGTG AGACTAGAAG AGGACAGATA 
CTTCTGTGGT CAGCCAATAA GGTTTTTGAA GAACTTACTG ACATAGAGAG ACAGTTTCAC 
AAAGCGCTTT ACACAGTCCG CGCCTTCCTT AACTGTGACA GATACTCTGT GGGACTGCTG 
GATATGACTA AAACTAAGGA ATTTTTTGAC CAATGGCCAA TACTCATGGG AGAAGTCCCC 
CCATATTCAG GACCCAAGAC TCCTGATGGG AGGGAAATAT CATTCTACAA AGTGATTGAC 
TATATTTTAC ATGGAAAAGA AGAAATTAAA GTTATACCGA ACCCCCCAGC AGATCACTGG 
TCTCTGGTTA GCAAACTACC AAGTTACGTT GCTGAAAACG GTCTTATTTG CAATATCATG 
AACACAAGTG CTGATGAATA CTTTGATTTT CAGAAAGGTC CGGTTGACGA CTCTGGGTGG 
ATTATGAAAA ATGTCCTTTC CATGCCAATC GTCAACAAGA AAGAAGAAAT TCTTGGCGTT 
GCAACGTTTT ACAACAGAAA GGATGGAAAA CCTTTTGATG AAATGGACGA AGTTTTGACA 
GAATCCTTAA CACAGTTCCT GGGATGGTCT GTCCTAAATA CTGATACGTA TGAGAAAATG 
AACAAACTCG AGAACAGAAA GGATATTTAC CAAGACATGG TCAACTATCA TGTGAAATGT 
TCAAACGATG AACTGCAGAT TATATTGAAA ACTAAGGAAA TCTATAACAG GCTGCCAGAA 
GAGTGCGAAG AAGAAGAATT ATATGAAATC TTGCAAGAAG AGCTTCCTGA CCCAACAGAA 
GCCGAAATTT ATGAATTCCA CTTCAGCGAC TTACCAGTCT CTGAACTTTT CTTGGTCAAA 
TGCGCAATTC AAATATATTA TGAGCTGGAA GTGGTAGACA AGTTCCATAT ACCACAAGAG 
GTATTAGTCC GCTTTTTATT TTCTGTAAGC AAAGGATACC GGAAAATAAC CTATCACAAC 
TGGCGGCATG GCTTTAATGT CGGACAGACA ATGTTTACAC TTCTCATGAC TGGAAACCTA 
AAGAGGTATT ATACAGATCT GGAGGCCATG GCTATGGTAA CAGCAGCTTT CTGCCATGAC 
ATTGACCACA GAGGAACTAA TAACTTGTAC CAGATGAAGT CTGGGCATCC ATTAGCAAAG 
CTCCATGGAT CCTCAATTCT AGAAAGGCAT CATTTGGAAT TTGGCAAATT TTTACTGAAA 
GATGAGTCAT TGAATATCTT CCAAAACCTC AACAGGAGGC AGTACGACCA TGCAATCCAC 
ATGATGGATA TTGCAATCAT TGCAACTGAT TTAGCCTTGT ATTTTAAAAA GAGGGCAATG 
TTTCAGAAGA TAGTTGACCA GTCTCAAACA TTCACAAGTG AAAAAGATTG GACAGAATAT 
ATGATGCTGG AACAGACCCG TAAAGAAATT GTCATGGCAA TGATGATGAC AGCTTGTGAT 
CTCTCAGCCA TAGCCAAGCC CTGGGATATA CAAAGCAAGG TTGCGTTGTT GGTTGCTGCA 
GAATTCTGGG AACAAGGTGA TTTGGAAAGG ACTGTATTGC AACAAAACCC AATCCCAATG 
ATGGACAGGA ACAAAGCAGA TGAACTTCCT AAGCTTCAGT GTGGTTTCAT TGACTTTGTG 
TGCACGTTTG TATATAAGGA ATTTTCTAGG TTCCATCCTG AGATACAGCC AATGTATGAT 
GGGATTCTAA ATAATAAGAA GGAGTGGAAA AAATTGGCTG ATGAATATGA TGCAAAAATG 
AAAACCCTGG AAGAGGACAA GCAAAAAAAG GAGGGGATAC AGCCACCAAA ACAAGTTCCA 
CAACCTACAG TGAGAGAAAA TGGAGGAAAC GTCCCAGTTA CCGAAGCAGC ACCACAGTCC 
AAAAGTTGTT CTATCTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde6a ( XM_002936704.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936750.3 Xenopus tropicalis phosphodiesterase 6A (pde6a), mRNA.

Price & Availability↑

CloneID	OXa54526
Clone ID Related Accession (Same CDS sequence)	XM_002936704.4
Accession Version	XM_002936704.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936704.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCTA TAACGCAGGC GGAAGTGGAG AAATTTCTGG ATTCCAACCC ATCTTTTGCC 
AAACAGTACT ACGACCTTAA ATTTAGACCC GGTATAATCT GTGATCTGCT CAGCACTGAA 
AGGACCAATG TGGACTTCTC CCACGTTCAT ACTTTGAGCA GTGTGGAGGA GAGCGAAATT 
GTCTTTGACC TCATCAGAGG ATTCCAGGAG AACCTCAATC TTGAAAGATG TGTTTTCAGT 
GTGCTCAAGA TGATGTCTTT CATAATTCAG GCAGATCGCA TGAGTTTGTT CATGTACCGC 
ATGAGGAATG GCACCGCTGA GCTTGCCACC AGGCTTTTTA ATGTTAATAA AGATGCCACC 
TTGGACGAAT GTGTGGTGGT TCCAGACTCT GAGATTGTGT TTCCTCTGGA TACTGGAGTA 
TTGGGCTATG TGGCACAGTC TAAGAAATTA GTGAATGTTT CAGATGTGAC ACAGGATTCT 
CATTTCTGCA GCTTTGTAGA TAACCTTACA GAGTATGACA CCAAAAGCAT CCTGGCTGCT 
CCTGTCCTGA ATGGAAAGGA TGTTGTCGCT GTAATAATGG CTGTCAATAA ATCAGATGGC 
ACCAATTTCA CCAAAACTGA TGAAGAGATG TTCCTTAAGT ACCTGAGTTT TGCCAATCTG 
GTTCTCAAGG TTTATCACCT TTCCTACCTT CACAACTGTG AGACTAGAAG AGGACAGATA 
CTTCTGTGGT CAGCCAATAA GGTTTTTGAA GAACTTACTG ACATAGAGAG ACAGTTTCAC 
AAAGCGCTTT ACACAGTCCG CGCCTTCCTT AACTGTGACA GATACTCTGT GGGACTGCTG 
GATATGACTA AAACTAAGGA ATTTTTTGAC CAATGGCCAA TACTCATGGG AGAAGTCCCC 
CCATATTCAG GACCCAAGAC TCCTGATGGG AGGGAAATAT CATTCTACAA AGTGATTGAC 
TATATTTTAC ATGGAAAAGA AGAAATTAAA GTTATACCGA ACCCCCCAGC AGATCACTGG 
TCTCTGGTTA GCAAACTACC AAGTTACGTT GCTGAAAACG GTCTTATTTG CAATATCATG 
AACACAAGTG CTGATGAATA CTTTGATTTT CAGAAAGGTC CGGTTGACGA CTCTGGGTGG 
ATTATGAAAA ATGTCCTTTC CATGCCAATC GTCAACAAGA AAGAAGAAAT TCTTGGAGTT 
GCAACGTTTT ACAACAGAAA GGATGGAAAA CCTTTTGATG AAATGGACGA AGTTTTGACA 
GAATCCTTAA CACAGTTCCT GGGATGGTCT GTCCTAAATA CTGATACGTA TGAGAAAATG 
AACAAACTCG AGAACAGAAA GGATATTTAC CAAGACATGG TCAACTATCA TGTGAAATGT 
TCAAACGATG AATTGCAGAT TATATTGAAA ACTAAGGAAA TCTATAACAG GCTGCCAGAA 
GAGTGCGAAG AAGAAGAATT ATATGAAATC TTGCAAGAAG AGCTTCCTGA CCCAACAGAA 
GCCGAAATTT ATGAATTCCA CTTCAGCGAC TTACCAGTCT CTGAACTTTT CTTGGTCAAA 
TGCGCAATTC AAATATATTA TGAGCTGGAA GTGGTAGACA AGTTCCATAT ACCACAAGAG 
GTATTAGTCC GCTTTTTATT TTCTGTAAGC AAAGGATACC GGAAAATAAC CTATCACAAC 
TGGCGGCATG GCTTTAATGT CGGACAGACA ATGTTTACAC TTCTCATGAC TGGAAACCTA 
AAGAGGTATT ATACAGATCT GGAGGCCATG GCTATGGTAA CAGCAGCTTT CTGCCATGAC 
ATTGACCACA GAGGAACTAA TAACTTGTAC CAGATGAAGT CTGGGCATCC ATTAGCAAAG 
CTCCATGGAT CCTCAATTCT AGAAAGGCAT CATTTGGAAT TTGGCAAATT TTTACTGAAA 
GATGAGTCAT TGAATATCTT CCAAAACCTC AACAGGAGGC AGTACGACCA TGCAATCCAC 
ATGATGGATA TTGCAATCAT TGCAACTGAT TTAGCCTTGT ATTTTAAAAA GAGGGCAATG 
TTTCAGAAGA TAGTTGACCA GTCTCAAACA TTCACAAGTG AAAAAGATTG GACAGAATAT 
ATGATGCTGG AACAGACCCG TAAAGAAATT GTCATGGCAA TGATGATGAC AGCTTGTGAT 
CTCTCAGCCA TAGCCAAGCC CTGGGATATA CAAAGCAAGG TTGCGTTGTT GGTTGCTGCA 
GAATTCTGGG AACAAGGTGA TTTGGAAAGG ACTGTATTGC AACAAAACCC AATCCCAATG 
ATGGACAGGA ACAAAGCAGA TGAACTTCCT AAGCTTCAGT GTGGTTTCAT TGACTTTGTG 
TGCACGTTTG TATATAAGGA ATTTTCTAGG TTCCATCCTG AGATACAGCC AATGTATGAT 
GGGATTCTAA ATAATAAGAA GGAGTGGAAA AAATTGGCTG ATGAATATGA TGCAAAAATG 
AAAACCCTGG AAGAGGACAA GCAAAAAAAG GAGGGGATAC AGCCACCAAA ACAAGTTCCA 
CAACCTACAG TGAGAGAAAA TGGAGGAAAC GTCCCAGTTA CCGAAGCAGC ACCACAGTCC 
AAAAGTTGTT CTATCTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936750.3
CDS	234..2834
Translation	MSSITQAEVEKFLDSNPSFAKQYYDLKFRPGIICDLLSTERTNVDFSHVHTLSSVEESEIVFDLIRGFQENLNLERCVFSVLKMMSFIIQADRMSLFMYRMRNGTAELATRLFNVNKDATLDECVVVPDSEIVFPLDTGVLGYVAQSKKLVNVSDVTQDSHFCSFVDNLTEYDTKSILAAPVLNGKDVVAVIMAVNKSDGTNFTKTDEEMFLKYLSFANLVLKVYHLSYLHNCETRRGQILLWSANKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLLDMTKTKEFFDQWPILMGEVPPYSGPKTPDGREISFYKVIDYILHGKEEIKVIPNPPADHWSLVSKLPSYVAENGLICNIMNTSADEYFDFQKGPVDDSGWIMKNVLSMPIVNKKEEILGVATFYNRKDGKPFDEMDEVLTESLTQFLGWSVLNTDTYEKMNKLENRKDIYQDMVNYHVKCSNDELQIILKTKEIYNRLPEECEEEELYEILQEELPDPTEAEIYEFHFSDLPVSELFLVKCAIQIYYELEVVDKFHIPQEVLVRFLFSVSKGYRKITYHNWRHGFNVGQTMFTLLMTGNLKRYYTDLEAMAMVTAAFCHDIDHRGTNNLYQMKSGHPLAKLHGSSILERHHLEFGKFLLKDESLNIFQNLNRRQYDHAIHMMDIAIIATDLALYFKKRAMFQKIVDQSQTFTSEKDWTEYMMLEQTRKEIVMAMMMTACDLSAIAKPWDIQSKVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELPKLQCGFIDFVCTFVYKEFSRFHPEIQPMYDGILNNKKEWKKLADEYDAKMKTLEEDKQKKEGIQPPKQVPQPTVRENGGNVPVTEAAPQSKSCSIL

Target ORF information:

RefSeq Version	XM_002936704.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 6A (pde6a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936704.4

ORF Insert Sequence:

ATGAGTTCTA TAACGCAGGC GGAAGTGGAG AAATTTCTGG ATTCCAACCC ATCTTTTGCC 
AAACAGTACT ACGACCTTAA ATTTAGACCC GGTATAATCT GTGATCTGCT CAGCACTGAA 
AGGACCAATG TGGACTTCTC CCACGTTCAT ACTTTGAGCA GTGTGGAGGA GAGCGAAATT 
GTCTTTGACC TCATCAGAGG ATTCCAGGAG AACCTCAATC TTGAAAGATG TGTTTTCAGT 
GTGCTCAAGA TGATGTCTTT CATAATTCAG GCAGATCGCA TGAGTTTGTT CATGTACCGC 
ATGAGGAATG GCACCGCTGA GCTTGCCACC AGGCTTTTTA ATGTTAATAA AGATGCCACC 
TTGGACGAAT GTGTGGTGGT TCCAGACTCT GAGATTGTGT TTCCTCTGGA TACTGGAGTA 
TTGGGCTATG TGGCACAGTC TAAGAAATTA GTGAATGTTT CAGATGTGAC ACAGGATTCT 
CATTTCTGCA GCTTTGTAGA TAACCTTACA GAGTATGACA CCAAAAGCAT CCTGGCTGCT 
CCTGTCCTGA ATGGAAAGGA TGTTGTCGCT GTAATAATGG CTGTCAATAA ATCAGATGGC 
ACCAATTTCA CCAAAACTGA TGAAGAGATG TTCCTTAAGT ACCTGAGTTT TGCCAATCTG 
GTTCTCAAGG TTTATCACCT TTCCTACCTT CACAACTGTG AGACTAGAAG AGGACAGATA 
CTTCTGTGGT CAGCCAATAA GGTTTTTGAA GAACTTACTG ACATAGAGAG ACAGTTTCAC 
AAAGCGCTTT ACACAGTCCG CGCCTTCCTT AACTGTGACA GATACTCTGT GGGACTGCTG 
GATATGACTA AAACTAAGGA ATTTTTTGAC CAATGGCCAA TACTCATGGG AGAAGTCCCC 
CCATATTCAG GACCCAAGAC TCCTGATGGG AGGGAAATAT CATTCTACAA AGTGATTGAC 
TATATTTTAC ATGGAAAAGA AGAAATTAAA GTTATACCGA ACCCCCCAGC AGATCACTGG 
TCTCTGGTTA GCAAACTACC AAGTTACGTT GCTGAAAACG GTCTTATTTG CAATATCATG 
AACACAAGTG CTGATGAATA CTTTGATTTT CAGAAAGGTC CGGTTGACGA CTCTGGGTGG 
ATTATGAAAA ATGTCCTTTC CATGCCAATC GTCAACAAGA AAGAAGAAAT TCTTGGAGTT 
GCAACGTTTT ACAACAGAAA GGATGGAAAA CCTTTTGATG AAATGGACGA AGTTTTGACA 
GAATCCTTAA CACAGTTCCT GGGATGGTCT GTCCTAAATA CTGATACGTA TGAGAAAATG 
AACAAACTCG AGAACAGAAA GGATATTTAC CAAGACATGG TCAACTATCA TGTGAAATGT 
TCAAACGATG AATTGCAGAT TATATTGAAA ACTAAGGAAA TCTATAACAG GCTGCCAGAA 
GAGTGCGAAG AAGAAGAATT ATATGAAATC TTGCAAGAAG AGCTTCCTGA CCCAACAGAA 
GCCGAAATTT ATGAATTCCA CTTCAGCGAC TTACCAGTCT CTGAACTTTT CTTGGTCAAA 
TGCGCAATTC AAATATATTA TGAGCTGGAA GTGGTAGACA AGTTCCATAT ACCACAAGAG 
GTATTAGTCC GCTTTTTATT TTCTGTAAGC AAAGGATACC GGAAAATAAC CTATCACAAC 
TGGCGGCATG GCTTTAATGT CGGACAGACA ATGTTTACAC TTCTCATGAC TGGAAACCTA 
AAGAGGTATT ATACAGATCT GGAGGCCATG GCTATGGTAA CAGCAGCTTT CTGCCATGAC 
ATTGACCACA GAGGAACTAA TAACTTGTAC CAGATGAAGT CTGGGCATCC ATTAGCAAAG 
CTCCATGGAT CCTCAATTCT AGAAAGGCAT CATTTGGAAT TTGGCAAATT TTTACTGAAA 
GATGAGTCAT TGAATATCTT CCAAAACCTC AACAGGAGGC AGTACGACCA TGCAATCCAC 
ATGATGGATA TTGCAATCAT TGCAACTGAT TTAGCCTTGT ATTTTAAAAA GAGGGCAATG 
TTTCAGAAGA TAGTTGACCA GTCTCAAACA TTCACAAGTG AAAAAGATTG GACAGAATAT 
ATGATGCTGG AACAGACCCG TAAAGAAATT GTCATGGCAA TGATGATGAC AGCTTGTGAT 
CTCTCAGCCA TAGCCAAGCC CTGGGATATA CAAAGCAAGG TTGCGTTGTT GGTTGCTGCA 
GAATTCTGGG AACAAGGTGA TTTGGAAAGG ACTGTATTGC AACAAAACCC AATCCCAATG 
ATGGACAGGA ACAAAGCAGA TGAACTTCCT AAGCTTCAGT GTGGTTTCAT TGACTTTGTG 
TGCACGTTTG TATATAAGGA ATTTTCTAGG TTCCATCCTG AGATACAGCC AATGTATGAT 
GGGATTCTAA ATAATAAGAA GGAGTGGAAA AAATTGGCTG ATGAATATGA TGCAAAAATG 
AAAACCCTGG AAGAGGACAA GCAAAAAAAG GAGGGGATAC AGCCACCAAA ACAAGTTCCA 
CAACCTACAG TGAGAGAAAA TGGAGGAAAC GTCCCAGTTA CCGAAGCAGC ACCACAGTCC 
AAAAGTTGTT CTATCTTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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pde6a cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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