vars1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	vars1
Entrez Gene ID	100489760
Full Name	valyl-tRNA synthetase
Synonyms	vars2
General protein information	Preferred Names valyl-tRNA synthetase Names valine--tRNA ligase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002939466.3	XP_002939512.2	valine--tRNA ligase
XM_004916911.3	XP_004916968.2	valine--tRNA ligase
XM_004916912.4	XP_004916969.2	valine--tRNA ligase
XM_004916913.4	XP_004916970.2	valine--tRNA ligase
XM_004916911.2	XP_004916968.1	valine--tRNA ligase
XM_004916912.3	XP_004916969.1	valine--tRNA ligase
XM_004916913.3	XP_004916970.1	valine--tRNA ligase
XM_002939466.4	XP_002939512.3	valine--tRNA ligase

Pathways from BioSystems

Homologies

	VARS	XP_001105797.2
Drosophila melanogaster (fruit fly)	Aats-val	NP_001260946.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010420	XP_311528.4
Schizosaccharomyces pombe (fission yeast)	vrs1	NP_595435.1
Homo sapiens (human)	VARS	NP_006286.1
Bos taurus (cattle)	VARS	XP_002697393.2
Mus musculus (house mouse)	Vars	NP_035820.3
Magnaporthe oryzae (rice blast fungus)	MGG_04396	XP_003711020.1
Neurospora crassa	NCU01965	XP_964329.2
Canis lupus familiaris (dog)	VARS	XP_005627219.1
Rattus norvegicus (Norway rat)	Vars	NP_445744.1
Danio rerio (zebrafish)	vars	XP_682807.5
Saccharomyces cerevisiae (baker's yeast)	VAS1	NP_011608.1
Arabidopsis thaliana (thale cress)	TWN2	NP_172913.1
Oryza sativa (rice)	Os03g0694900	NP_001050973.1
Oryza sativa (rice)	Os10g0506200	NP_001176231.1
Xenopus tropicalis (tropical clawed frog)	vars	XP_002939512.2
Caenorhabditis elegans (roundworm)	vars-2	NP_493377.1
Kluyveromyces lactis	KLLA0D14971g	XP_453724.1
Pan troglodytes (chimpanzee)	VARS	XP_518361.2
Eremothecium gossypii	AGOS_AAR034W	NP_982575.1

Gene Ontology

The following vars1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vars1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa63915	XM_002939466.4 Latest version!	Xenopus tropicalis valyl-tRNA synthetase 1 (vars1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63915	XM_004916913.4 Latest version!	Xenopus tropicalis valyl-tRNA synthetase 1 (vars1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63915	XM_004916912.4 Latest version!	Xenopus tropicalis valyl-tRNA synthetase 1 (vars1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa63915	XM_004916911.3 Latest version!	Xenopus tropicalis valyl-tRNA synthetase 1 (vars1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa14446	XM_004916912.3 Latest version!	Xenopus tropicalis valyl-tRNA synthetase (vars), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa14446	XM_004916913.3 Latest version!	Xenopus tropicalis valyl-tRNA synthetase (vars), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa14446	XM_002939466.3 Latest version!	Xenopus tropicalis valyl-tRNA synthetase (vars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa14446	XM_004916911.2 Latest version!	Xenopus tropicalis valyl-tRNA synthetase (vars), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vars1 ( XM_004916911.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916968.2 Xenopus tropicalis valyl-tRNA synthetase 1 (vars1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa63915
Clone ID Related Accession (Same CDS sequence)	XM_004916911.3 , XM_004916912.4 , XM_004916913.4 , XM_002939466.4
Accession Version	XM_004916911.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916911.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTATGTAAG CCAAAAGTAC AGGAGGTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAAGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GGCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATG CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916968.2
CDS	100..3831
Translation	MSTLYLPNSTSSDPRSLWVRIAGQYSTLCKPKVQEVSSGPHGLPLPCLLSPSNEPVWGSWAVSYMLAPEALSGGCDPCSGALVKQWVSYAESELLPAVYGAERSQTKDRSLLELQKILDSLDFYLRLRTYLVGESISLADIAVACCLISPYTKVLSPTRREKLLNVTRWFQTCINQQQFKDILGEVNFLKTDVAASKTSDGPSGTPSNAPPKTAAQLKKESKKKEKLERFQQKQEKIKELQGEKKPKPEKKDKKELGVITYDIPTPAGDKKDVSGPMPDSYSPQYVESAWYSWWEKEKFFKPEFGRSSISEPNPKGLFMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGELTLWNPGCDHAGIATQVVVEKKLWRERGKTRHDFGRENFIEEVWKWKREKGDRIYHQLRILGSSLDWDRTCFTMDPKLSFAVQEAFIRLHEAGIIYRSKRLVNWSCTLNSAISDIEVDKKELSGRTLLPVPGYKQGVEFGVLVSFAYKVQETGEEIVVATTRVETMLGDTAVAVHPQDQRYKHLKGHHVLHPFCSRLLPIVFDEFVDMSFGTGAVKITPAHDPTDYEVGMRHSLDFVNIMDDNGTLINVPQPFLGMKRFDARKAVLDALKEKGLFKEVKDNPMVVPVCSRSKDIVEPLLKPQWYVRCDEMGKRAADAVRDGHLLIKPEFHTKTWFIWMDNIRDWCISRQLWWGHRIPAYFVTVNDPSVPAGEDTDGKYWISGRSEQEAKEKAARVFNVAPEKISLRQDDDVLDTWFSSGIFPFSIFGWPNQTEDLNVFYPGTLLETGHDILFFWVARMVMLGLTLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVINGITLEGLNKQLLESNLDPAELERAKDGQKSDYPNGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKIWNATKFAMKGLGDNYSPPDFAGACGQESLADRWILSRLSFAVDLCNNGFQNYDFPGITTAVYNFWLYELCDVYLECLKPVFLSSDEAAISVGRNTLYTCLDAGLRLLSPFMPFLTEELYQRLPRRSSSRTPSISVTSYPETSECNWRDEEIERNMDLALLIVKSIRSLRADYNLTKTKADCFVQCQDADTVSVVTAYTSFITVLSSSRSLVTLKAQDPAPAGSAVNTASDKATVYVVLKGLIDVEKELAKLQVKKTELGRQLEKLRERMGSADYRSKVPETVQQQDAERIRQTETEFKKVNEAAENFQKMV

Target ORF information:

RefSeq Version	XM_004916911.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis valyl-tRNA synthetase 1 (vars1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916911.3

ORF Insert Sequence:

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTATGTAAG CCAAAAGTAC AGGAGGTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAAGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GGCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATG CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vars1 ( XM_004916912.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916969.2 Xenopus tropicalis valyl-tRNA synthetase 1 (vars1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa63915
Clone ID Related Accession (Same CDS sequence)	XM_004916911.3 , XM_004916912.4 , XM_004916913.4 , XM_002939466.4
Accession Version	XM_004916912.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916912.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTATGTAAG CCAAAAGTAC AGGAGGTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAAGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GGCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATG CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916969.2
CDS	136..3867
Translation	MSTLYLPNSTSSDPRSLWVRIAGQYSTLCKPKVQEVSSGPHGLPLPCLLSPSNEPVWGSWAVSYMLAPEALSGGCDPCSGALVKQWVSYAESELLPAVYGAERSQTKDRSLLELQKILDSLDFYLRLRTYLVGESISLADIAVACCLISPYTKVLSPTRREKLLNVTRWFQTCINQQQFKDILGEVNFLKTDVAASKTSDGPSGTPSNAPPKTAAQLKKESKKKEKLERFQQKQEKIKELQGEKKPKPEKKDKKELGVITYDIPTPAGDKKDVSGPMPDSYSPQYVESAWYSWWEKEKFFKPEFGRSSISEPNPKGLFMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGELTLWNPGCDHAGIATQVVVEKKLWRERGKTRHDFGRENFIEEVWKWKREKGDRIYHQLRILGSSLDWDRTCFTMDPKLSFAVQEAFIRLHEAGIIYRSKRLVNWSCTLNSAISDIEVDKKELSGRTLLPVPGYKQGVEFGVLVSFAYKVQETGEEIVVATTRVETMLGDTAVAVHPQDQRYKHLKGHHVLHPFCSRLLPIVFDEFVDMSFGTGAVKITPAHDPTDYEVGMRHSLDFVNIMDDNGTLINVPQPFLGMKRFDARKAVLDALKEKGLFKEVKDNPMVVPVCSRSKDIVEPLLKPQWYVRCDEMGKRAADAVRDGHLLIKPEFHTKTWFIWMDNIRDWCISRQLWWGHRIPAYFVTVNDPSVPAGEDTDGKYWISGRSEQEAKEKAARVFNVAPEKISLRQDDDVLDTWFSSGIFPFSIFGWPNQTEDLNVFYPGTLLETGHDILFFWVARMVMLGLTLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVINGITLEGLNKQLLESNLDPAELERAKDGQKSDYPNGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKIWNATKFAMKGLGDNYSPPDFAGACGQESLADRWILSRLSFAVDLCNNGFQNYDFPGITTAVYNFWLYELCDVYLECLKPVFLSSDEAAISVGRNTLYTCLDAGLRLLSPFMPFLTEELYQRLPRRSSSRTPSISVTSYPETSECNWRDEEIERNMDLALLIVKSIRSLRADYNLTKTKADCFVQCQDADTVSVVTAYTSFITVLSSSRSLVTLKAQDPAPAGSAVNTASDKATVYVVLKGLIDVEKELAKLQVKKTELGRQLEKLRERMGSADYRSKVPETVQQQDAERIRQTETEFKKVNEAAENFQKMV

Target ORF information:

RefSeq Version	XM_004916912.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis valyl-tRNA synthetase 1 (vars1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916912.4

ORF Insert Sequence:

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTATGTAAG CCAAAAGTAC AGGAGGTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAAGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GGCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATG CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vars1 ( XM_004916913.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916970.2 Xenopus tropicalis valyl-tRNA synthetase 1 (vars1), transcript variant X4, mRNA.

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CloneID	OXa63915
Clone ID Related Accession (Same CDS sequence)	XM_004916911.3 , XM_004916912.4 , XM_004916913.4 , XM_002939466.4
Accession Version	XM_004916913.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916913.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTATGTAAG CCAAAAGTAC AGGAGGTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAAGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GGCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATG CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916970.2
CDS	36..3767
Translation	MSTLYLPNSTSSDPRSLWVRIAGQYSTLCKPKVQEVSSGPHGLPLPCLLSPSNEPVWGSWAVSYMLAPEALSGGCDPCSGALVKQWVSYAESELLPAVYGAERSQTKDRSLLELQKILDSLDFYLRLRTYLVGESISLADIAVACCLISPYTKVLSPTRREKLLNVTRWFQTCINQQQFKDILGEVNFLKTDVAASKTSDGPSGTPSNAPPKTAAQLKKESKKKEKLERFQQKQEKIKELQGEKKPKPEKKDKKELGVITYDIPTPAGDKKDVSGPMPDSYSPQYVESAWYSWWEKEKFFKPEFGRSSISEPNPKGLFMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGELTLWNPGCDHAGIATQVVVEKKLWRERGKTRHDFGRENFIEEVWKWKREKGDRIYHQLRILGSSLDWDRTCFTMDPKLSFAVQEAFIRLHEAGIIYRSKRLVNWSCTLNSAISDIEVDKKELSGRTLLPVPGYKQGVEFGVLVSFAYKVQETGEEIVVATTRVETMLGDTAVAVHPQDQRYKHLKGHHVLHPFCSRLLPIVFDEFVDMSFGTGAVKITPAHDPTDYEVGMRHSLDFVNIMDDNGTLINVPQPFLGMKRFDARKAVLDALKEKGLFKEVKDNPMVVPVCSRSKDIVEPLLKPQWYVRCDEMGKRAADAVRDGHLLIKPEFHTKTWFIWMDNIRDWCISRQLWWGHRIPAYFVTVNDPSVPAGEDTDGKYWISGRSEQEAKEKAARVFNVAPEKISLRQDDDVLDTWFSSGIFPFSIFGWPNQTEDLNVFYPGTLLETGHDILFFWVARMVMLGLTLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVINGITLEGLNKQLLESNLDPAELERAKDGQKSDYPNGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKIWNATKFAMKGLGDNYSPPDFAGACGQESLADRWILSRLSFAVDLCNNGFQNYDFPGITTAVYNFWLYELCDVYLECLKPVFLSSDEAAISVGRNTLYTCLDAGLRLLSPFMPFLTEELYQRLPRRSSSRTPSISVTSYPETSECNWRDEEIERNMDLALLIVKSIRSLRADYNLTKTKADCFVQCQDADTVSVVTAYTSFITVLSSSRSLVTLKAQDPAPAGSAVNTASDKATVYVVLKGLIDVEKELAKLQVKKTELGRQLEKLRERMGSADYRSKVPETVQQQDAERIRQTETEFKKVNEAAENFQKMV

Target ORF information:

RefSeq Version	XM_004916913.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis valyl-tRNA synthetase 1 (vars1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916913.4

ORF Insert Sequence:

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTATGTAAG CCAAAAGTAC AGGAGGTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAAGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GGCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATG CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vars1 ( XM_002939466.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939512.3 Xenopus tropicalis valyl-tRNA synthetase 1 (vars1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa63915
Clone ID Related Accession (Same CDS sequence)	XM_004916911.3 , XM_004916912.4 , XM_004916913.4 , XM_002939466.4
Accession Version	XM_002939466.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002939466.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTATGTAAG CCAAAAGTAC AGGAGGTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAAGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GGCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATG CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939512.3
CDS	104..3835
Translation	MSTLYLPNSTSSDPRSLWVRIAGQYSTLCKPKVQEVSSGPHGLPLPCLLSPSNEPVWGSWAVSYMLAPEALSGGCDPCSGALVKQWVSYAESELLPAVYGAERSQTKDRSLLELQKILDSLDFYLRLRTYLVGESISLADIAVACCLISPYTKVLSPTRREKLLNVTRWFQTCINQQQFKDILGEVNFLKTDVAASKTSDGPSGTPSNAPPKTAAQLKKESKKKEKLERFQQKQEKIKELQGEKKPKPEKKDKKELGVITYDIPTPAGDKKDVSGPMPDSYSPQYVESAWYSWWEKEKFFKPEFGRSSISEPNPKGLFMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGELTLWNPGCDHAGIATQVVVEKKLWRERGKTRHDFGRENFIEEVWKWKREKGDRIYHQLRILGSSLDWDRTCFTMDPKLSFAVQEAFIRLHEAGIIYRSKRLVNWSCTLNSAISDIEVDKKELSGRTLLPVPGYKQGVEFGVLVSFAYKVQETGEEIVVATTRVETMLGDTAVAVHPQDQRYKHLKGHHVLHPFCSRLLPIVFDEFVDMSFGTGAVKITPAHDPTDYEVGMRHSLDFVNIMDDNGTLINVPQPFLGMKRFDARKAVLDALKEKGLFKEVKDNPMVVPVCSRSKDIVEPLLKPQWYVRCDEMGKRAADAVRDGHLLIKPEFHTKTWFIWMDNIRDWCISRQLWWGHRIPAYFVTVNDPSVPAGEDTDGKYWISGRSEQEAKEKAARVFNVAPEKISLRQDDDVLDTWFSSGIFPFSIFGWPNQTEDLNVFYPGTLLETGHDILFFWVARMVMLGLTLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVINGITLEGLNKQLLESNLDPAELERAKDGQKSDYPNGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKIWNATKFAMKGLGDNYSPPDFAGACGQESLADRWILSRLSFAVDLCNNGFQNYDFPGITTAVYNFWLYELCDVYLECLKPVFLSSDEAAISVGRNTLYTCLDAGLRLLSPFMPFLTEELYQRLPRRSSSRTPSISVTSYPETSECNWRDEEIERNMDLALLIVKSIRSLRADYNLTKTKADCFVQCQDADTVSVVTAYTSFITVLSSSRSLVTLKAQDPAPAGSAVNTASDKATVYVVLKGLIDVEKELAKLQVKKTELGRQLEKLRERMGSADYRSKVPETVQQQDAERIRQTETEFKKVNEAAENFQKMV

Target ORF information:

RefSeq Version	XM_002939466.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis valyl-tRNA synthetase 1 (vars1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939466.4

ORF Insert Sequence:

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTATGTAAG CCAAAAGTAC AGGAGGTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAAGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GGCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATG CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vars1 ( XM_002939466.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002939512.2 Xenopus tropicalis valyl-tRNA synthetase (vars), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa14446
Clone ID Related Accession (Same CDS sequence)	XM_002939466.3 , XM_004916911.2 , XM_004916912.3 , XM_004916913.3
Accession Version	XM_002939466.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_002939466.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTGTGTAAG CCAAAAGTAC AGGAGTTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAGGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GCCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATT CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002939512.2
CDS	104..3835
Translation	MSTLYLPNSTSSDPRSLWVRIAGQYSTLCKPKVQELSSGPHGLPLPCLLSPSNEPVWGSWAVSYMLAPEALSGGCDPCSGALVKQWVSYAESELLPAVYGAERSQTKDRSLLELQKILDSLDFYLRLRTYLVGESISLADIAVACCLISPYTKVLSPTRREKLLNVTRWFQTCINQQQFKDILGEVNFLKTDVAASKTSDGPSGTPSNAPPKTAAQLKKESKKKEKLERFQQKQEKIKELQGEKKPKPEKKDKKELGVITYDIPTPAGDKKDVSGPMPDSYSPQYVESAWYSWWEKEKFFKPEFGRSSISEPNPKGLFMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGELTLWNPGCDHAGIATQVVVEKKLWRERGKTRHDFGRENFIEEVWKWKREKGDRIYHQLRILGSSLDWDRTCFTMDPKLSFAVQEAFIRLHEAGIIYRSKRLVNWSCTLNSAISDIEVDKKELSGRTLLPVPGYKQGVEFGVLVSFAYKVQETGEEIVVATTRVETMLGDTAVAVHPQDQRYKHLKGHHVLHPFCSRLLPIVFDEFVDMSFGTGAVKITPAHDPTDYEVGMRHSLDFVNIMDDNGTLINVPQPFLGMKRFDARKAVLDALKEKGLFKEVKDNPMVVPVCSRSKDIVEPLLKPQWYVRCDEMGKRAADAVRDGHLLIKPEFHTKTWFIWMDNIRDWCISRQLWWGHRIPAYFVTVNDPSVPAGEDTDGKYWISGRSEQEAKEKAARVFNVAPEKISLRQDDDVLDTWFSSGIFPFSIFGWPNQTEDLNVFYPGTLLETGHDILFFWVARMVMLGLTLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVINGITLEGLNKQLLESNLDPAELERAKDGQKSDYPNGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKIWNATKFAMKGLGDNYSPPDFAGACGQESLADRWILSRLSFAVDLCNNGFQNYDFPGITTAVYNFWLYELCDVYLECLKPVFLSSDEAAISVGRNTLYTCLDSGLRLLSPFMPFLTEELYQRLPRRSSSRTPSISVTSYPETSECNWRDEEIERNMDLALLIVKSIRSLRADYNLTKTKADCFVQCQDADTVSVVTAYTSFITVLSSSRSLVTLKAQDPAPAGSAVNTASDKATVYVVLKGLIDVEKELAKLQVKKTELGRQLEKLRERMGSADYRSKVPETVQQQDAERIRQTETEFKKVNEAAENFQKMV

Target ORF information:

RefSeq Version	XM_002939466.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis valyl-tRNA synthetase (vars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002939466.3

ORF Insert Sequence:

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTGTGTAAG CCAAAAGTAC AGGAGTTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAGGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GCCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATT CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vars1 ( XM_004916911.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916968.1 Xenopus tropicalis valyl-tRNA synthetase (vars), transcript variant X2, mRNA.

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CloneID	OXa14446
Clone ID Related Accession (Same CDS sequence)	XM_002939466.3 , XM_004916911.2 , XM_004916912.3 , XM_004916913.3
Accession Version	XM_004916911.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004916911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTGTGTAAG CCAAAAGTAC AGGAGTTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAGGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GCCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATT CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916968.1
CDS	100..3831
Translation	MSTLYLPNSTSSDPRSLWVRIAGQYSTLCKPKVQELSSGPHGLPLPCLLSPSNEPVWGSWAVSYMLAPEALSGGCDPCSGALVKQWVSYAESELLPAVYGAERSQTKDRSLLELQKILDSLDFYLRLRTYLVGESISLADIAVACCLISPYTKVLSPTRREKLLNVTRWFQTCINQQQFKDILGEVNFLKTDVAASKTSDGPSGTPSNAPPKTAAQLKKESKKKEKLERFQQKQEKIKELQGEKKPKPEKKDKKELGVITYDIPTPAGDKKDVSGPMPDSYSPQYVESAWYSWWEKEKFFKPEFGRSSISEPNPKGLFMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGELTLWNPGCDHAGIATQVVVEKKLWRERGKTRHDFGRENFIEEVWKWKREKGDRIYHQLRILGSSLDWDRTCFTMDPKLSFAVQEAFIRLHEAGIIYRSKRLVNWSCTLNSAISDIEVDKKELSGRTLLPVPGYKQGVEFGVLVSFAYKVQETGEEIVVATTRVETMLGDTAVAVHPQDQRYKHLKGHHVLHPFCSRLLPIVFDEFVDMSFGTGAVKITPAHDPTDYEVGMRHSLDFVNIMDDNGTLINVPQPFLGMKRFDARKAVLDALKEKGLFKEVKDNPMVVPVCSRSKDIVEPLLKPQWYVRCDEMGKRAADAVRDGHLLIKPEFHTKTWFIWMDNIRDWCISRQLWWGHRIPAYFVTVNDPSVPAGEDTDGKYWISGRSEQEAKEKAARVFNVAPEKISLRQDDDVLDTWFSSGIFPFSIFGWPNQTEDLNVFYPGTLLETGHDILFFWVARMVMLGLTLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVINGITLEGLNKQLLESNLDPAELERAKDGQKSDYPNGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKIWNATKFAMKGLGDNYSPPDFAGACGQESLADRWILSRLSFAVDLCNNGFQNYDFPGITTAVYNFWLYELCDVYLECLKPVFLSSDEAAISVGRNTLYTCLDSGLRLLSPFMPFLTEELYQRLPRRSSSRTPSISVTSYPETSECNWRDEEIERNMDLALLIVKSIRSLRADYNLTKTKADCFVQCQDADTVSVVTAYTSFITVLSSSRSLVTLKAQDPAPAGSAVNTASDKATVYVVLKGLIDVEKELAKLQVKKTELGRQLEKLRERMGSADYRSKVPETVQQQDAERIRQTETEFKKVNEAAENFQKMV

Target ORF information:

RefSeq Version	XM_004916911.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis valyl-tRNA synthetase (vars), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916911.2

ORF Insert Sequence:

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTGTGTAAG CCAAAAGTAC AGGAGTTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAGGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GCCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATT CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vars1 ( XM_004916912.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916969.1 Xenopus tropicalis valyl-tRNA synthetase (vars), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa14446
Clone ID Related Accession (Same CDS sequence)	XM_002939466.3 , XM_004916911.2 , XM_004916912.3 , XM_004916913.3
Accession Version	XM_004916912.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916912.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTGTGTAAG CCAAAAGTAC AGGAGTTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAGGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GCCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATT CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916969.1
CDS	143..3874
Translation	MSTLYLPNSTSSDPRSLWVRIAGQYSTLCKPKVQELSSGPHGLPLPCLLSPSNEPVWGSWAVSYMLAPEALSGGCDPCSGALVKQWVSYAESELLPAVYGAERSQTKDRSLLELQKILDSLDFYLRLRTYLVGESISLADIAVACCLISPYTKVLSPTRREKLLNVTRWFQTCINQQQFKDILGEVNFLKTDVAASKTSDGPSGTPSNAPPKTAAQLKKESKKKEKLERFQQKQEKIKELQGEKKPKPEKKDKKELGVITYDIPTPAGDKKDVSGPMPDSYSPQYVESAWYSWWEKEKFFKPEFGRSSISEPNPKGLFMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGELTLWNPGCDHAGIATQVVVEKKLWRERGKTRHDFGRENFIEEVWKWKREKGDRIYHQLRILGSSLDWDRTCFTMDPKLSFAVQEAFIRLHEAGIIYRSKRLVNWSCTLNSAISDIEVDKKELSGRTLLPVPGYKQGVEFGVLVSFAYKVQETGEEIVVATTRVETMLGDTAVAVHPQDQRYKHLKGHHVLHPFCSRLLPIVFDEFVDMSFGTGAVKITPAHDPTDYEVGMRHSLDFVNIMDDNGTLINVPQPFLGMKRFDARKAVLDALKEKGLFKEVKDNPMVVPVCSRSKDIVEPLLKPQWYVRCDEMGKRAADAVRDGHLLIKPEFHTKTWFIWMDNIRDWCISRQLWWGHRIPAYFVTVNDPSVPAGEDTDGKYWISGRSEQEAKEKAARVFNVAPEKISLRQDDDVLDTWFSSGIFPFSIFGWPNQTEDLNVFYPGTLLETGHDILFFWVARMVMLGLTLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVINGITLEGLNKQLLESNLDPAELERAKDGQKSDYPNGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKIWNATKFAMKGLGDNYSPPDFAGACGQESLADRWILSRLSFAVDLCNNGFQNYDFPGITTAVYNFWLYELCDVYLECLKPVFLSSDEAAISVGRNTLYTCLDSGLRLLSPFMPFLTEELYQRLPRRSSSRTPSISVTSYPETSECNWRDEEIERNMDLALLIVKSIRSLRADYNLTKTKADCFVQCQDADTVSVVTAYTSFITVLSSSRSLVTLKAQDPAPAGSAVNTASDKATVYVVLKGLIDVEKELAKLQVKKTELGRQLEKLRERMGSADYRSKVPETVQQQDAERIRQTETEFKKVNEAAENFQKMV

Target ORF information:

RefSeq Version	XM_004916912.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis valyl-tRNA synthetase (vars), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916912.3

ORF Insert Sequence:

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTGTGTAAG CCAAAAGTAC AGGAGTTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAGGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GCCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATT CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vars1 ( XM_004916913.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916970.1 Xenopus tropicalis valyl-tRNA synthetase (vars), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa14446
Clone ID Related Accession (Same CDS sequence)	XM_002939466.3 , XM_004916911.2 , XM_004916912.3 , XM_004916913.3
Accession Version	XM_004916913.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	valine--tRNA ligase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916913.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTGTGTAAG CCAAAAGTAC AGGAGTTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAGGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GCCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATT CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916970.1
CDS	89..3820
Translation	MSTLYLPNSTSSDPRSLWVRIAGQYSTLCKPKVQELSSGPHGLPLPCLLSPSNEPVWGSWAVSYMLAPEALSGGCDPCSGALVKQWVSYAESELLPAVYGAERSQTKDRSLLELQKILDSLDFYLRLRTYLVGESISLADIAVACCLISPYTKVLSPTRREKLLNVTRWFQTCINQQQFKDILGEVNFLKTDVAASKTSDGPSGTPSNAPPKTAAQLKKESKKKEKLERFQQKQEKIKELQGEKKPKPEKKDKKELGVITYDIPTPAGDKKDVSGPMPDSYSPQYVESAWYSWWEKEKFFKPEFGRSSISEPNPKGLFMMCIPPPNVTGSLHLGHALTNAIQDCLTRWHRMRGELTLWNPGCDHAGIATQVVVEKKLWRERGKTRHDFGRENFIEEVWKWKREKGDRIYHQLRILGSSLDWDRTCFTMDPKLSFAVQEAFIRLHEAGIIYRSKRLVNWSCTLNSAISDIEVDKKELSGRTLLPVPGYKQGVEFGVLVSFAYKVQETGEEIVVATTRVETMLGDTAVAVHPQDQRYKHLKGHHVLHPFCSRLLPIVFDEFVDMSFGTGAVKITPAHDPTDYEVGMRHSLDFVNIMDDNGTLINVPQPFLGMKRFDARKAVLDALKEKGLFKEVKDNPMVVPVCSRSKDIVEPLLKPQWYVRCDEMGKRAADAVRDGHLLIKPEFHTKTWFIWMDNIRDWCISRQLWWGHRIPAYFVTVNDPSVPAGEDTDGKYWISGRSEQEAKEKAARVFNVAPEKISLRQDDDVLDTWFSSGIFPFSIFGWPNQTEDLNVFYPGTLLETGHDILFFWVARMVMLGLTLTGKLPFKEVYLHAVVRDAHGRKMSKSLGNVIDPLDVINGITLEGLNKQLLESNLDPAELERAKDGQKSDYPNGIPECGTDALRFALCAYTSQGRDINLDVNRILGYRHFCNKIWNATKFAMKGLGDNYSPPDFAGACGQESLADRWILSRLSFAVDLCNNGFQNYDFPGITTAVYNFWLYELCDVYLECLKPVFLSSDEAAISVGRNTLYTCLDSGLRLLSPFMPFLTEELYQRLPRRSSSRTPSISVTSYPETSECNWRDEEIERNMDLALLIVKSIRSLRADYNLTKTKADCFVQCQDADTVSVVTAYTSFITVLSSSRSLVTLKAQDPAPAGSAVNTASDKATVYVVLKGLIDVEKELAKLQVKKTELGRQLEKLRERMGSADYRSKVPETVQQQDAERIRQTETEFKKVNEAAENFQKMV

Target ORF information:

RefSeq Version	XM_004916913.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis valyl-tRNA synthetase (vars), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916913.3

ORF Insert Sequence:

ATGTCTACCC TCTATCTTCC GAACTCCACC TCATCAGACC CGCGATCTCT GTGGGTGCGC 
ATTGCTGGAC AGTATTCTAC CCTGTGTAAG CCAAAAGTAC AGGAGTTGTC TTCTGGCCCT 
CACGGACTAC CACTTCCTTG TTTGCTCTCA CCCTCCAATG AACCAGTATG GGGTTCTTGG 
GCTGTTTCAT ATATGCTTGC TCCTGAAGCA CTTTCTGGAG GATGTGACCC CTGCTCCGGT 
GCTCTTGTTA AACAGTGGGT GAGCTATGCA GAGTCAGAGT TGTTGCCTGC TGTGTATGGA 
GCAGAACGTT CACAGACTAA AGATCGTTCT CTTCTGGAGC TGCAGAAGAT TCTGGATAGT 
CTTGATTTTT ATCTTAGACT TCGCACATAT CTTGTGGGAG AGAGCATCAG TCTTGCAGAC 
ATTGCTGTAG CGTGTTGTTT GATCAGTCCC TATACAAAAG TTCTTTCTCC CACAAGAAGA 
GAGAAACTTC TTAATGTTAC TCGGTGGTTC CAAACTTGCA TTAATCAACA GCAGTTTAAA 
GATATTCTAG GGGAGGTTAA CTTTCTAAAA ACAGATGTTG CTGCTTCAAA AACTTCAGAT 
GGTCCTTCTG GTACCCCTTC CAATGCCCCC CCTAAAACTG CAGCCCAATT AAAGAAGGAG 
TCTAAGAAGA AAGAAAAACT GGAGCGGTTC CAACAAAAGC AAGAGAAAAT AAAAGAACTG 
CAGGGGGAGA AAAAGCCCAA ACCTGAAAAG AAAGACAAGA AAGAACTTGG GGTGATTACA 
TATGATATTC CTACTCCTGC AGGTGATAAA AAAGATGTTT CTGGTCCTAT GCCTGACTCC 
TACAGTCCCC AGTATGTAGA ATCTGCTTGG TACTCATGGT GGGAAAAAGA GAAGTTTTTC 
AAGCCCGAAT TTGGGAGAAG CAGCATTTCA GAGCCCAACC CAAAGGGTTT GTTCATGATG 
TGTATTCCTC CACCAAATGT GACTGGATCA CTTCATCTTG GACATGCTCT TACTAATGCA 
ATCCAGGACT GTTTGACCCG CTGGCATCGT ATGCGAGGGG AGCTAACCCT CTGGAACCCT 
GGCTGTGACC ATGCAGGTAT TGCTACTCAA GTAGTGGTGG AGAAGAAGCT GTGGAGAGAG 
CGTGGGAAGA CCAGACATGA CTTTGGAAGA GAGAATTTTA TAGAGGAGGT GTGGAAATGG 
AAAAGAGAGA AAGGTGACAG AATATACCAC CAACTGAGGA TTCTTGGAAG TTCTCTGGAC 
TGGGATAGAA CATGTTTTAC AATGGATCCT AAACTTTCCT TTGCTGTACA AGAAGCTTTT 
ATCCGCTTGC ATGAGGCTGG AATAATTTAT CGAAGCAAAC GTCTAGTCAA CTGGTCCTGC 
ACTCTTAATT CTGCTATATC AGACATAGAG GTAGACAAGA AGGAACTGAG TGGTCGTACT 
CTACTACCTG TGCCTGGCTA TAAGCAGGGT GTGGAATTTG GTGTACTTGT CTCGTTTGCT 
TACAAAGTTC AAGAAACAGG TGAGGAAATT GTTGTGGCAA CAACTCGAGT GGAGACAATG 
CTTGGGGACA CGGCAGTTGC TGTACATCCT CAGGACCAAA GGTACAAGCA TTTAAAGGGT 
CATCACGTTC TCCATCCATT TTGCTCTCGC CTCCTTCCTA TCGTCTTTGA TGAGTTTGTA 
GACATGAGCT TTGGAACAGG TGCTGTAAAA ATCACTCCTG CCCATGATCC AACTGATTAT 
GAGGTTGGAA TGAGACACTC TCTAGATTTT GTTAACATCA TGGATGACAA TGGGACTCTT 
ATAAATGTAC CCCAACCTTT CCTGGGAATG AAAAGATTTG ATGCCAGAAA AGCTGTTTTG 
GATGCACTAA AAGAAAAGGG ACTTTTCAAG GAGGTCAAAG ACAACCCTAT GGTTGTGCCA 
GTTTGCAGCC GTTCAAAGGA CATTGTAGAG CCTTTGCTGA AGCCTCAGTG GTATGTACGT 
TGTGATGAGA TGGGGAAAAG AGCTGCTGAT GCTGTGCGTG ATGGACATTT GCTAATTAAA 
CCTGAATTCC ACACTAAGAC ATGGTTCATT TGGATGGACA ATATCAGGGA CTGGTGCATT 
TCACGTCAAC TGTGGTGGGG CCACCGTATC CCAGCTTACT TTGTGACAGT TAATGATCCA 
TCTGTACCTG CTGGAGAGGA TACAGATGGA AAGTACTGGA TCAGTGGTCG CTCTGAACAA 
GAAGCCAAAG AGAAAGCAGC AAGAGTATTT AATGTAGCGC CCGAAAAAAT ATCTTTGAGG 
CAAGACGATG ATGTGCTTGA CACCTGGTTT TCTTCTGGGA TTTTTCCCTT TTCCATCTTT 
GGCTGGCCCA ATCAGACTGA AGACCTTAAC GTTTTCTACC CTGGCACCCT ACTGGAGACT 
GGCCATGATA TTCTCTTCTT CTGGGTGGCT CGGATGGTGA TGCTTGGTTT AACCTTAACT 
GGTAAACTCC CATTTAAAGA GGTGTACCTC CATGCTGTAG TAAGGGACGC TCATGGTCGA 
AAGATGAGCA AGTCACTGGG AAACGTCATT GATCCTTTGG ATGTGATCAA TGGCATCACT 
CTGGAAGGTC TCAACAAACA ACTTTTAGAA AGTAACCTAG ACCCTGCAGA GCTGGAGAGA 
GCCAAGGATG GCCAGAAATC TGATTACCCT AATGGCATCC CAGAATGTGG AACGGATGCT 
TTGAGGTTTG CACTATGTGC TTATACTAGT CAAGGTCGGG ACATTAATCT GGATGTGAAC 
AGGATTCTTG GTTATCGTCA TTTCTGCAAT AAAATCTGGA ATGCAACTAA GTTTGCAATG 
AAAGGTCTGG GAGATAATTA TTCTCCACCT GACTTTGCAG GGGCTTGTGG ACAAGAAAGC 
CTTGCTGACC GATGGATATT GAGCCGCCTT AGTTTTGCAG TTGATCTCTG TAATAATGGT 
TTTCAAAACT ATGACTTTCC TGGCATTACA ACAGCTGTGT ACAACTTCTG GCTTTACGAG 
TTGTGTGATG TTTATCTGGA GTGTTTGAAG CCTGTATTTT TAAGCTCTGA TGAGGCTGCA 
ATATCTGTGG GACGGAACAC GCTGTACACC TGCTTGGATT CTGGCCTTCG TCTTCTCTCT 
CCCTTCATGC CATTTCTTAC TGAGGAATTA TATCAGCGCC TTCCCCGGCG CTCCAGCAGT 
AGAACTCCAA GTATTTCAGT CACTTCCTAC CCAGAGACAT CAGAATGCAA CTGGCGAGAT 
GAGGAAATTG AGAGAAATAT GGATTTGGCA CTGCTCATTG TCAAGTCAAT CCGATCTCTG 
CGAGCAGACT ATAATCTCAC TAAAACAAAG GCTGACTGTT TCGTTCAATG TCAAGATGCT 
GACACCGTTT CTGTGGTTAC TGCTTACACT TCATTCATCA CGGTCCTCTC TTCCTCACGC 
TCTCTGGTTA CTCTGAAGGC TCAGGATCCT GCTCCAGCCG GAAGCGCTGT TAATACTGCA 
TCAGATAAAG CTACTGTGTA TGTGGTGCTC AAGGGCCTAA TAGATGTAGA GAAGGAGCTG 
GCCAAACTGC AAGTTAAGAA GACTGAATTG GGCAGACAGC TAGAGAAGCT GCGTGAGAGG 
ATGGGCAGTG CAGATTACCG CAGCAAAGTG CCCGAAACAG TGCAGCAACA AGATGCAGAG 
CGGATCAGAC AGACTGAGAC AGAGTTCAAG AAGGTGAATG AGGCTGCAGA GAACTTTCAG 
AAAATGGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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vars1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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