guf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	guf1
Entrez Gene ID	100489624
Full Name	GUF1 homolog, GTPase
General protein information	Preferred Names GUF1 homolog, GTPase Names translation factor GUF1, mitochondrial GTP-binding protein GUF1 homolog GUF1 GTPase homolog
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004911213.3	XP_004911270.1	translation factor GUF1, mitochondrial
XM_004911213.4	XP_004911270.1	translation factor GUF1, mitochondrial
XM_002933449.4	XP_002933495.1	translation factor GUF1, mitochondrial
XM_012955693.3	XP_012811147.1	translation factor GUF1, mitochondrial
XM_012955693.2	XP_012811147.1	translation factor GUF1, mitochondrial
XM_002933449.5	XP_002933495.1	translation factor GUF1, mitochondrial

Pathways from BioSystems

Homologies

Homo sapiens (human)	GUF1	NP_068746.2
Mus musculus (house mouse)	Guf1	NP_766299.1
Danio rerio (zebrafish)	guf1	XP_002664348.1
Caenorhabditis elegans (roundworm)	CELE_ZK1236.1	NP_498863.1
Schizosaccharomyces pombe (fission yeast)	SPAC1B3.04c	NP_594788.2
Drosophila melanogaster (fruit fly)	waw	NP_001027089.1
Eremothecium gossypii	AGOS_AGR290W	NP_986956.1
Pan troglodytes (chimpanzee)	GUF1	XP_517175.2
Canis lupus familiaris (dog)	GUF1	XP_539246.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009971	XP_319107.3
Kluyveromyces lactis	KLLA0C04862g	XP_452417.1
Arabidopsis thaliana (thale cress)	AT5G39900	NP_198806.2
	GUF1	XP_001099650.1
Magnaporthe oryzae (rice blast fungus)	MGG_04686	XP_003710846.1
Neurospora crassa	NCU05927	XP_958122.1
Bos taurus (cattle)	GUF1	NP_001095537.1
Rattus norvegicus (Norway rat)	Guf1	NP_001100685.1
Gallus gallus (chicken)	GUF1	XP_004936120.1
Saccharomyces cerevisiae (baker's yeast)	GUF1	NP_013392.1
Oryza sativa (rice)	Os06g0144800	NP_001056783.1
Xenopus tropicalis (tropical clawed frog)	guf1	XP_002933495.1

Gene Ontology

The following guf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the guf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04737	XM_002933449.5 Latest version!	Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa04737	XM_012955693.3 Latest version!	Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa04737	XM_004911213.4 Latest version!	Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OXa04737	XM_004911213.3	Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa04737	XM_012955693.2	Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa04737	XM_002933449.4	Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

guf1 ( XM_004911213.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911270.1 Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa04737
Clone ID Related Accession (Same CDS sequence)	XM_004911213.3 , XM_004911213.4 , XM_002933449.4 , XM_012955693.3 , XM_012955693.2 , XM_002933449.5
Accession Version	XM_004911213.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation factor GUF1, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911213.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCC TGTTTCCTCA TTCTACTTCA AAATGTCTGC GCTTGTGGAG ACACACGATT 
TCTGCCCAGC AGAGGCACCT GGCATCTCAG AACTTGTTCG GTACCACTCT GTTTGGCCTT 
CACCACTTGC AGAAAACTGT TAACCCTATG AATATTCTGA GTCCAAGGCA ATTTAGTTCT 
CAGAAGGAAA AACTTGACAT GAGTGAATAT GCAGTTGACT ATATAAGGAA TTTTTGCATA 
ATTGCCCATG TTGACCATGG TAAAAGTACT CTTGCTGACA GACTGCTTGA AATAACAGGG 
CGGATTCCAA AAACAGATTC AAATAAACAA GTTTTGGACA AGCTCCAGGT GGAGCGAGAG 
AGAGGAATTA CTGTCAAGGC ACAGACTGCG TCTCTCTTTT ATACTTTTGA AGGAAAAAAG 
TACCTCTTAA ACCTGATAGA CACACCAGGT CACGTGGATT TCAGCTATGA AGTATCTAGG 
TCCCTGTCTG CCTGTCAAGG TGTTTTGCTT GTGGTTGATG CCAATGAGGG TATTCAAGCA 
CAGACTGTGG CAAACTTCTT CCTAGCCTTT GAAGCACAGC TTTCAATTAT TCCCGTCATA 
AACAAGATTG ATTTGAAGAA TGCAGACCCT GAAAGAGCAG AAAAGCAAAT TGAAAAGATG 
TTTGACATTC CAAGAAGTGA CTGCATAAGG ATCTCTGCTA AATTTGGAAC AAATGTGGAG 
CGGGTTCTGC AAGAGGTTAT TACCAAAATT CCCCCGCCCA AGATGGAGAG AATGAGCCCC 
CTAAAAGCTT TACTGTTTGA CTCCACCTTT GATCACTACA GAGGAGTTGT GGCTAATGTT 
GCACTGTTTG GCGGGGAGGT AACCAAAGGC CACAAGATTC TCTCCGCACA CACGGGGAAG 
AGCTATGAAG TCAATGAAGT TGGTATCCTG ACTCCGGAAG AAGTGCCAGC AGATAAATTA 
TATGCTGGGC AGGTGGGATA CCTCATTGCA GGGATGAAAG AAGTAAAAGA AGCACAAATA 
GGAGACACTC TGTATCTGCA GAAACAGCCG GTAGAGCCCC TGCCTGGGTT CAAATCTGCC 
AAGCCCATGG TGTTTGCTGG CATGTATCCT GTTGACCAGT CTGAATACAA TAACTTGAAG 
GGGGCGTTAG AGAAACTGAC ATTAAATGAC TCTAGTGTCA CAGTGCATCG GGACAGCAGT 
GCTGCACTTG GGGCTGGTTG GAGGTTGGGA TTCCTTGGGT TGTTGCACCT GGAAGTATTT 
AACCAGCGCT TAGAGCAGGA ATACAATGCG TCAGTAATAA TGACTTCACC AACTGTTCCA 
TACAGGGCCA TCCTTTCTTC TCCCAAACTA ATTAAGGAGT ATGGAGAAAA GGAGATAACA 
ATAGTAAACC CTGCAGAATT CCCAGACAAG TCTCAGGTGT TGGAATATCT AGAGCCCATG 
GTATTGGGAA CAATCATTTC ACCGGACGAA TTCATGAGCA AAATCCTATC TCTGTGTCAG 
TCACGAAGAG GAATTCAGAA AAGCCTGGTG TATATCGACG ATCACAGAAT CATGATGAAA 
TATCTTTTTC CTTTGAATGA AATTGTAGTA GATTTTTACG ATGGACTAAA GTCTCTGTCA 
TCTGGATATG CAAGCTTTGA TTATGAAGAC GCAGGGTACC AGTCAGCTGA CCTAGTGAAA 
ATGGACATCC TGCTAAACGG GCGCCCCGTG GAAGAGCTGG TCACTATTGC CCACAGGGAA 
AAGGCCTACC CAGTTGGAAA GGCAATATGT GAAAGGCTGT GCGAGTCCAT CCCCAGGCAG 
TTGTTTGAAA TAGCCATCCA AGCTTCTATC GGTAGCAAAG TCATAGCAAG AGAAACAGTT 
AAAGCTTACA GAAAGAATGT CCTGGCAAAA TGCTACGGAG GTGATATCAC AAGGAAAATG 
AAGCTGCTGA AGAGGCAAGC TGAGGGAAAG AAGAAAATGA GAAAAATCGG CAATGTGGAA 
GTGCCAAAAG ATGCTTTTAT CAGTGTGTTG AAACGAAAAC CAGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911270.1
CDS	193..2223
Translation	MSSLFPHSTSKCLRLWRHTISAQQRHLASQNLFGTTLFGLHHLQKTVNPMNILSPRQFSSQKEKLDMSEYAVDYIRNFCIIAHVDHGKSTLADRLLEITGRIPKTDSNKQVLDKLQVERERGITVKAQTASLFYTFEGKKYLLNLIDTPGHVDFSYEVSRSLSACQGVLLVVDANEGIQAQTVANFFLAFEAQLSIIPVINKIDLKNADPERAEKQIEKMFDIPRSDCIRISAKFGTNVERVLQEVITKIPPPKMERMSPLKALLFDSTFDHYRGVVANVALFGGEVTKGHKILSAHTGKSYEVNEVGILTPEEVPADKLYAGQVGYLIAGMKEVKEAQIGDTLYLQKQPVEPLPGFKSAKPMVFAGMYPVDQSEYNNLKGALEKLTLNDSSVTVHRDSSAALGAGWRLGFLGLLHLEVFNQRLEQEYNASVIMTSPTVPYRAILSSPKLIKEYGEKEITIVNPAEFPDKSQVLEYLEPMVLGTIISPDEFMSKILSLCQSRRGIQKSLVYIDDHRIMMKYLFPLNEIVVDFYDGLKSLSSGYASFDYEDAGYQSADLVKMDILLNGRPVEELVTIAHREKAYPVGKAICERLCESIPRQLFEIAIQASIGSKVIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKKMRKIGNVEVPKDAFISVLKRKPDK

Target ORF information:

RefSeq Version	XM_004911213.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911213.3

ORF Insert Sequence:

ATGTCCTCCC TGTTTCCTCA TTCTACTTCA AAATGTCTGC GCTTGTGGAG ACACACGATT 
TCTGCCCAGC AGAGGCACCT GGCATCTCAG AACTTGTTCG GTACCACTCT GTTTGGCCTT 
CACCACTTGC AGAAAACTGT TAACCCTATG AATATTCTGA GTCCAAGGCA ATTTAGTTCT 
CAGAAGGAAA AACTTGACAT GAGTGAATAT GCAGTTGACT ATATAAGGAA TTTTTGCATA 
ATTGCCCATG TTGACCATGG TAAAAGTACT CTTGCTGACA GACTGCTTGA AATAACAGGG 
CGGATTCCAA AAACAGATTC AAATAAACAA GTTTTGGACA AGCTCCAGGT GGAGCGAGAG 
AGAGGAATTA CTGTCAAGGC ACAGACTGCG TCTCTCTTTT ATACTTTTGA AGGAAAAAAG 
TACCTCTTAA ACCTGATAGA CACACCAGGT CACGTGGATT TCAGCTATGA AGTATCTAGG 
TCCCTGTCTG CCTGTCAAGG TGTTTTGCTT GTGGTTGATG CCAATGAGGG TATTCAAGCA 
CAGACTGTGG CAAACTTCTT CCTAGCCTTT GAAGCACAGC TTTCAATTAT TCCCGTCATA 
AACAAGATTG ATTTGAAGAA TGCAGACCCT GAAAGAGCAG AAAAGCAAAT TGAAAAGATG 
TTTGACATTC CAAGAAGTGA CTGCATAAGG ATCTCTGCTA AATTTGGAAC AAATGTGGAG 
CGGGTTCTGC AAGAGGTTAT TACCAAAATT CCCCCGCCCA AGATGGAGAG AATGAGCCCC 
CTAAAAGCTT TACTGTTTGA CTCCACCTTT GATCACTACA GAGGAGTTGT GGCTAATGTT 
GCACTGTTTG GCGGGGAGGT AACCAAAGGC CACAAGATTC TCTCCGCACA CACGGGGAAG 
AGCTATGAAG TCAATGAAGT TGGTATCCTG ACTCCGGAAG AAGTGCCAGC AGATAAATTA 
TATGCTGGGC AGGTGGGATA CCTCATTGCA GGGATGAAAG AAGTAAAAGA AGCACAAATA 
GGAGACACTC TGTATCTGCA GAAACAGCCG GTAGAGCCCC TGCCTGGGTT CAAATCTGCC 
AAGCCCATGG TGTTTGCTGG CATGTATCCT GTTGACCAGT CTGAATACAA TAACTTGAAG 
GGGGCGTTAG AGAAACTGAC ATTAAATGAC TCTAGTGTCA CAGTGCATCG GGACAGCAGT 
GCTGCACTTG GGGCTGGTTG GAGGTTGGGA TTCCTTGGGT TGTTGCACCT GGAAGTATTT 
AACCAGCGCT TAGAGCAGGA ATACAATGCG TCAGTAATAA TGACTTCACC AACTGTTCCA 
TACAGGGCCA TCCTTTCTTC TCCCAAACTA ATTAAGGAGT ATGGAGAAAA GGAGATAACA 
ATAGTAAACC CTGCAGAATT CCCAGACAAG TCTCAGGTGT TGGAATATCT AGAGCCCATG 
GTATTGGGAA CAATCATTTC ACCGGACGAA TTCATGAGCA AAATCCTATC TCTGTGTCAG 
TCACGAAGAG GAATTCAGAA AAGCCTGGTG TATATCGACG ATCACAGAAT CATGATGAAA 
TATCTTTTTC CTTTGAATGA AATTGTAGTA GATTTTTACG ATGGACTAAA GTCTCTGTCA 
TCTGGATATG CAAGCTTTGA TTATGAAGAC GCAGGGTACC AGTCAGCTGA CCTAGTGAAA 
ATGGACATCC TGCTAAACGG GCGCCCCGTG GAAGAGCTGG TCACTATTGC CCACAGGGAA 
AAGGCCTACC CAGTTGGAAA GGCAATATGT GAAAGGCTGT GCGAGTCCAT CCCCAGGCAG 
TTGTTTGAAA TAGCCATCCA AGCTTCTATC GGTAGCAAAG TCATAGCAAG AGAAACAGTT 
AAAGCTTACA GAAAGAATGT CCTGGCAAAA TGCTACGGAG GTGATATCAC AAGGAAAATG 
AAGCTGCTGA AGAGGCAAGC TGAGGGAAAG AAGAAAATGA GAAAAATCGG CAATGTGGAA 
GTGCCAAAAG ATGCTTTTAT CAGTGTGTTG AAACGAAAAC CAGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

guf1 ( XM_002933449.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933495.1 Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa04737
Clone ID Related Accession (Same CDS sequence)	XM_004911213.3 , XM_004911213.4 , XM_002933449.4 , XM_012955693.3 , XM_012955693.2 , XM_002933449.5
Accession Version	XM_002933449.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation factor GUF1, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933449.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCC TGTTTCCTCA TTCTACTTCA AAATGTCTGC GCTTGTGGAG ACACACGATT 
TCTGCCCAGC AGAGGCACCT GGCATCTCAG AACTTGTTCG GTACCACTCT GTTTGGCCTT 
CACCACTTGC AGAAAACTGT TAACCCTATG AATATTCTGA GTCCAAGGCA ATTTAGTTCT 
CAGAAGGAAA AACTTGACAT GAGTGAATAT GCAGTTGACT ATATAAGGAA TTTTTGCATA 
ATTGCCCATG TTGACCATGG TAAAAGTACT CTTGCTGACA GACTGCTTGA AATAACAGGG 
CGGATTCCAA AAACAGATTC AAATAAACAA GTTTTGGACA AGCTCCAGGT GGAGCGAGAG 
AGAGGAATTA CTGTCAAGGC ACAGACTGCG TCTCTCTTTT ATACTTTTGA AGGAAAAAAG 
TACCTCTTAA ACCTGATAGA CACACCAGGT CACGTGGATT TCAGCTATGA AGTATCTAGG 
TCCCTGTCTG CCTGTCAAGG TGTTTTGCTT GTGGTTGATG CCAATGAGGG TATTCAAGCA 
CAGACTGTGG CAAACTTCTT CCTAGCCTTT GAAGCACAGC TTTCAATTAT TCCCGTCATA 
AACAAGATTG ATTTGAAGAA TGCAGACCCT GAAAGAGCAG AAAAGCAAAT TGAAAAGATG 
TTTGACATTC CAAGAAGTGA CTGCATAAGG ATCTCTGCTA AATTTGGAAC AAATGTGGAG 
CGGGTTCTGC AAGAGGTTAT TACCAAAATT CCCCCGCCCA AGATGGAGAG AATGAGCCCC 
CTAAAAGCTT TACTGTTTGA CTCCACCTTT GATCACTACA GAGGAGTTGT GGCTAATGTT 
GCACTGTTTG GCGGGGAGGT AACCAAAGGC CACAAGATTC TCTCCGCACA CACGGGGAAG 
AGCTATGAAG TCAATGAAGT TGGTATCCTG ACTCCGGAAG AAGTGCCAGC AGATAAATTA 
TATGCTGGGC AGGTGGGATA CCTCATTGCA GGGATGAAAG AAGTAAAAGA AGCACAAATA 
GGAGACACTC TGTATCTGCA GAAACAGCCG GTAGAGCCCC TGCCTGGGTT CAAATCTGCC 
AAGCCCATGG TGTTTGCTGG CATGTATCCT GTTGACCAGT CTGAATACAA TAACTTGAAG 
GGGGCGTTAG AGAAACTGAC ATTAAATGAC TCTAGTGTCA CAGTGCATCG GGACAGCAGT 
GCTGCACTTG GGGCTGGTTG GAGGTTGGGA TTCCTTGGGT TGTTGCACCT GGAAGTATTT 
AACCAGCGCT TAGAGCAGGA ATACAATGCG TCAGTAATAA TGACTTCACC AACTGTTCCA 
TACAGGGCCA TCCTTTCTTC TCCCAAACTA ATTAAGGAGT ATGGAGAAAA GGAGATAACA 
ATAGTAAACC CTGCAGAATT CCCAGACAAG TCTCAGGTGT TGGAATATCT AGAGCCCATG 
GTATTGGGAA CAATCATTTC ACCGGACGAA TTCATGAGCA AAATCCTATC TCTGTGTCAG 
TCACGAAGAG GAATTCAGAA AAGCCTGGTG TATATCGACG ATCACAGAAT CATGATGAAA 
TATCTTTTTC CTTTGAATGA AATTGTAGTA GATTTTTACG ATGGACTAAA GTCTCTGTCA 
TCTGGATATG CAAGCTTTGA TTATGAAGAC GCAGGGTACC AGTCAGCTGA CCTAGTGAAA 
ATGGACATCC TGCTAAACGG GCGCCCCGTG GAAGAGCTGG TCACTATTGC CCACAGGGAA 
AAGGCCTACC CAGTTGGAAA GGCAATATGT GAAAGGCTGT GCGAGTCCAT CCCCAGGCAG 
TTGTTTGAAA TAGCCATCCA AGCTTCTATC GGTAGCAAAG TCATAGCAAG AGAAACAGTT 
AAAGCTTACA GAAAGAATGT CCTGGCAAAA TGCTACGGAG GTGATATCAC AAGGAAAATG 
AAGCTGCTGA AGAGGCAAGC TGAGGGAAAG AAGAAAATGA GAAAAATCGG CAATGTGGAA 
GTGCCAAAAG ATGCTTTTAT CAGTGTGTTG AAACGAAAAC CAGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933495.1
CDS	204..2234
Translation	MSSLFPHSTSKCLRLWRHTISAQQRHLASQNLFGTTLFGLHHLQKTVNPMNILSPRQFSSQKEKLDMSEYAVDYIRNFCIIAHVDHGKSTLADRLLEITGRIPKTDSNKQVLDKLQVERERGITVKAQTASLFYTFEGKKYLLNLIDTPGHVDFSYEVSRSLSACQGVLLVVDANEGIQAQTVANFFLAFEAQLSIIPVINKIDLKNADPERAEKQIEKMFDIPRSDCIRISAKFGTNVERVLQEVITKIPPPKMERMSPLKALLFDSTFDHYRGVVANVALFGGEVTKGHKILSAHTGKSYEVNEVGILTPEEVPADKLYAGQVGYLIAGMKEVKEAQIGDTLYLQKQPVEPLPGFKSAKPMVFAGMYPVDQSEYNNLKGALEKLTLNDSSVTVHRDSSAALGAGWRLGFLGLLHLEVFNQRLEQEYNASVIMTSPTVPYRAILSSPKLIKEYGEKEITIVNPAEFPDKSQVLEYLEPMVLGTIISPDEFMSKILSLCQSRRGIQKSLVYIDDHRIMMKYLFPLNEIVVDFYDGLKSLSSGYASFDYEDAGYQSADLVKMDILLNGRPVEELVTIAHREKAYPVGKAICERLCESIPRQLFEIAIQASIGSKVIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKKMRKIGNVEVPKDAFISVLKRKPDK

Target ORF information:

RefSeq Version	XM_002933449.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933449.4

ORF Insert Sequence:

ATGTCCTCCC TGTTTCCTCA TTCTACTTCA AAATGTCTGC GCTTGTGGAG ACACACGATT 
TCTGCCCAGC AGAGGCACCT GGCATCTCAG AACTTGTTCG GTACCACTCT GTTTGGCCTT 
CACCACTTGC AGAAAACTGT TAACCCTATG AATATTCTGA GTCCAAGGCA ATTTAGTTCT 
CAGAAGGAAA AACTTGACAT GAGTGAATAT GCAGTTGACT ATATAAGGAA TTTTTGCATA 
ATTGCCCATG TTGACCATGG TAAAAGTACT CTTGCTGACA GACTGCTTGA AATAACAGGG 
CGGATTCCAA AAACAGATTC AAATAAACAA GTTTTGGACA AGCTCCAGGT GGAGCGAGAG 
AGAGGAATTA CTGTCAAGGC ACAGACTGCG TCTCTCTTTT ATACTTTTGA AGGAAAAAAG 
TACCTCTTAA ACCTGATAGA CACACCAGGT CACGTGGATT TCAGCTATGA AGTATCTAGG 
TCCCTGTCTG CCTGTCAAGG TGTTTTGCTT GTGGTTGATG CCAATGAGGG TATTCAAGCA 
CAGACTGTGG CAAACTTCTT CCTAGCCTTT GAAGCACAGC TTTCAATTAT TCCCGTCATA 
AACAAGATTG ATTTGAAGAA TGCAGACCCT GAAAGAGCAG AAAAGCAAAT TGAAAAGATG 
TTTGACATTC CAAGAAGTGA CTGCATAAGG ATCTCTGCTA AATTTGGAAC AAATGTGGAG 
CGGGTTCTGC AAGAGGTTAT TACCAAAATT CCCCCGCCCA AGATGGAGAG AATGAGCCCC 
CTAAAAGCTT TACTGTTTGA CTCCACCTTT GATCACTACA GAGGAGTTGT GGCTAATGTT 
GCACTGTTTG GCGGGGAGGT AACCAAAGGC CACAAGATTC TCTCCGCACA CACGGGGAAG 
AGCTATGAAG TCAATGAAGT TGGTATCCTG ACTCCGGAAG AAGTGCCAGC AGATAAATTA 
TATGCTGGGC AGGTGGGATA CCTCATTGCA GGGATGAAAG AAGTAAAAGA AGCACAAATA 
GGAGACACTC TGTATCTGCA GAAACAGCCG GTAGAGCCCC TGCCTGGGTT CAAATCTGCC 
AAGCCCATGG TGTTTGCTGG CATGTATCCT GTTGACCAGT CTGAATACAA TAACTTGAAG 
GGGGCGTTAG AGAAACTGAC ATTAAATGAC TCTAGTGTCA CAGTGCATCG GGACAGCAGT 
GCTGCACTTG GGGCTGGTTG GAGGTTGGGA TTCCTTGGGT TGTTGCACCT GGAAGTATTT 
AACCAGCGCT TAGAGCAGGA ATACAATGCG TCAGTAATAA TGACTTCACC AACTGTTCCA 
TACAGGGCCA TCCTTTCTTC TCCCAAACTA ATTAAGGAGT ATGGAGAAAA GGAGATAACA 
ATAGTAAACC CTGCAGAATT CCCAGACAAG TCTCAGGTGT TGGAATATCT AGAGCCCATG 
GTATTGGGAA CAATCATTTC ACCGGACGAA TTCATGAGCA AAATCCTATC TCTGTGTCAG 
TCACGAAGAG GAATTCAGAA AAGCCTGGTG TATATCGACG ATCACAGAAT CATGATGAAA 
TATCTTTTTC CTTTGAATGA AATTGTAGTA GATTTTTACG ATGGACTAAA GTCTCTGTCA 
TCTGGATATG CAAGCTTTGA TTATGAAGAC GCAGGGTACC AGTCAGCTGA CCTAGTGAAA 
ATGGACATCC TGCTAAACGG GCGCCCCGTG GAAGAGCTGG TCACTATTGC CCACAGGGAA 
AAGGCCTACC CAGTTGGAAA GGCAATATGT GAAAGGCTGT GCGAGTCCAT CCCCAGGCAG 
TTGTTTGAAA TAGCCATCCA AGCTTCTATC GGTAGCAAAG TCATAGCAAG AGAAACAGTT 
AAAGCTTACA GAAAGAATGT CCTGGCAAAA TGCTACGGAG GTGATATCAC AAGGAAAATG 
AAGCTGCTGA AGAGGCAAGC TGAGGGAAAG AAGAAAATGA GAAAAATCGG CAATGTGGAA 
GTGCCAAAAG ATGCTTTTAT CAGTGTGTTG AAACGAAAAC CAGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

guf1 ( XM_012955693.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811147.1 Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa04737
Clone ID Related Accession (Same CDS sequence)	XM_004911213.3 , XM_004911213.4 , XM_002933449.4 , XM_012955693.3 , XM_012955693.2 , XM_002933449.5
Accession Version	XM_012955693.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation factor GUF1, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCC TGTTTCCTCA TTCTACTTCA AAATGTCTGC GCTTGTGGAG ACACACGATT 
TCTGCCCAGC AGAGGCACCT GGCATCTCAG AACTTGTTCG GTACCACTCT GTTTGGCCTT 
CACCACTTGC AGAAAACTGT TAACCCTATG AATATTCTGA GTCCAAGGCA ATTTAGTTCT 
CAGAAGGAAA AACTTGACAT GAGTGAATAT GCAGTTGACT ATATAAGGAA TTTTTGCATA 
ATTGCCCATG TTGACCATGG TAAAAGTACT CTTGCTGACA GACTGCTTGA AATAACAGGG 
CGGATTCCAA AAACAGATTC AAATAAACAA GTTTTGGACA AGCTCCAGGT GGAGCGAGAG 
AGAGGAATTA CTGTCAAGGC ACAGACTGCG TCTCTCTTTT ATACTTTTGA AGGAAAAAAG 
TACCTCTTAA ACCTGATAGA CACACCAGGT CACGTGGATT TCAGCTATGA AGTATCTAGG 
TCCCTGTCTG CCTGTCAAGG TGTTTTGCTT GTGGTTGATG CCAATGAGGG TATTCAAGCA 
CAGACTGTGG CAAACTTCTT CCTAGCCTTT GAAGCACAGC TTTCAATTAT TCCCGTCATA 
AACAAGATTG ATTTGAAGAA TGCAGACCCT GAAAGAGCAG AAAAGCAAAT TGAAAAGATG 
TTTGACATTC CAAGAAGTGA CTGCATAAGG ATCTCTGCTA AATTTGGAAC AAATGTGGAG 
CGGGTTCTGC AAGAGGTTAT TACCAAAATT CCCCCGCCCA AGATGGAGAG AATGAGCCCC 
CTAAAAGCTT TACTGTTTGA CTCCACCTTT GATCACTACA GAGGAGTTGT GGCTAATGTT 
GCACTGTTTG GCGGGGAGGT AACCAAAGGC CACAAGATTC TCTCCGCACA CACGGGGAAG 
AGCTATGAAG TCAATGAAGT TGGTATCCTG ACTCCGGAAG AAGTGCCAGC AGATAAATTA 
TATGCTGGGC AGGTGGGATA CCTCATTGCA GGGATGAAAG AAGTAAAAGA AGCACAAATA 
GGAGACACTC TGTATCTGCA GAAACAGCCG GTAGAGCCCC TGCCTGGGTT CAAATCTGCC 
AAGCCCATGG TGTTTGCTGG CATGTATCCT GTTGACCAGT CTGAATACAA TAACTTGAAG 
GGGGCGTTAG AGAAACTGAC ATTAAATGAC TCTAGTGTCA CAGTGCATCG GGACAGCAGT 
GCTGCACTTG GGGCTGGTTG GAGGTTGGGA TTCCTTGGGT TGTTGCACCT GGAAGTATTT 
AACCAGCGCT TAGAGCAGGA ATACAATGCG TCAGTAATAA TGACTTCACC AACTGTTCCA 
TACAGGGCCA TCCTTTCTTC TCCCAAACTA ATTAAGGAGT ATGGAGAAAA GGAGATAACA 
ATAGTAAACC CTGCAGAATT CCCAGACAAG TCTCAGGTGT TGGAATATCT AGAGCCCATG 
GTATTGGGAA CAATCATTTC ACCGGACGAA TTCATGAGCA AAATCCTATC TCTGTGTCAG 
TCACGAAGAG GAATTCAGAA AAGCCTGGTG TATATCGACG ATCACAGAAT CATGATGAAA 
TATCTTTTTC CTTTGAATGA AATTGTAGTA GATTTTTACG ATGGACTAAA GTCTCTGTCA 
TCTGGATATG CAAGCTTTGA TTATGAAGAC GCAGGGTACC AGTCAGCTGA CCTAGTGAAA 
ATGGACATCC TGCTAAACGG GCGCCCCGTG GAAGAGCTGG TCACTATTGC CCACAGGGAA 
AAGGCCTACC CAGTTGGAAA GGCAATATGT GAAAGGCTGT GCGAGTCCAT CCCCAGGCAG 
TTGTTTGAAA TAGCCATCCA AGCTTCTATC GGTAGCAAAG TCATAGCAAG AGAAACAGTT 
AAAGCTTACA GAAAGAATGT CCTGGCAAAA TGCTACGGAG GTGATATCAC AAGGAAAATG 
AAGCTGCTGA AGAGGCAAGC TGAGGGAAAG AAGAAAATGA GAAAAATCGG CAATGTGGAA 
GTGCCAAAAG ATGCTTTTAT CAGTGTGTTG AAACGAAAAC CAGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811147.1
CDS	126..2156
Translation	MSSLFPHSTSKCLRLWRHTISAQQRHLASQNLFGTTLFGLHHLQKTVNPMNILSPRQFSSQKEKLDMSEYAVDYIRNFCIIAHVDHGKSTLADRLLEITGRIPKTDSNKQVLDKLQVERERGITVKAQTASLFYTFEGKKYLLNLIDTPGHVDFSYEVSRSLSACQGVLLVVDANEGIQAQTVANFFLAFEAQLSIIPVINKIDLKNADPERAEKQIEKMFDIPRSDCIRISAKFGTNVERVLQEVITKIPPPKMERMSPLKALLFDSTFDHYRGVVANVALFGGEVTKGHKILSAHTGKSYEVNEVGILTPEEVPADKLYAGQVGYLIAGMKEVKEAQIGDTLYLQKQPVEPLPGFKSAKPMVFAGMYPVDQSEYNNLKGALEKLTLNDSSVTVHRDSSAALGAGWRLGFLGLLHLEVFNQRLEQEYNASVIMTSPTVPYRAILSSPKLIKEYGEKEITIVNPAEFPDKSQVLEYLEPMVLGTIISPDEFMSKILSLCQSRRGIQKSLVYIDDHRIMMKYLFPLNEIVVDFYDGLKSLSSGYASFDYEDAGYQSADLVKMDILLNGRPVEELVTIAHREKAYPVGKAICERLCESIPRQLFEIAIQASIGSKVIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKKMRKIGNVEVPKDAFISVLKRKPDK

Target ORF information:

RefSeq Version	XM_012955693.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955693.2

ORF Insert Sequence:

ATGTCCTCCC TGTTTCCTCA TTCTACTTCA AAATGTCTGC GCTTGTGGAG ACACACGATT 
TCTGCCCAGC AGAGGCACCT GGCATCTCAG AACTTGTTCG GTACCACTCT GTTTGGCCTT 
CACCACTTGC AGAAAACTGT TAACCCTATG AATATTCTGA GTCCAAGGCA ATTTAGTTCT 
CAGAAGGAAA AACTTGACAT GAGTGAATAT GCAGTTGACT ATATAAGGAA TTTTTGCATA 
ATTGCCCATG TTGACCATGG TAAAAGTACT CTTGCTGACA GACTGCTTGA AATAACAGGG 
CGGATTCCAA AAACAGATTC AAATAAACAA GTTTTGGACA AGCTCCAGGT GGAGCGAGAG 
AGAGGAATTA CTGTCAAGGC ACAGACTGCG TCTCTCTTTT ATACTTTTGA AGGAAAAAAG 
TACCTCTTAA ACCTGATAGA CACACCAGGT CACGTGGATT TCAGCTATGA AGTATCTAGG 
TCCCTGTCTG CCTGTCAAGG TGTTTTGCTT GTGGTTGATG CCAATGAGGG TATTCAAGCA 
CAGACTGTGG CAAACTTCTT CCTAGCCTTT GAAGCACAGC TTTCAATTAT TCCCGTCATA 
AACAAGATTG ATTTGAAGAA TGCAGACCCT GAAAGAGCAG AAAAGCAAAT TGAAAAGATG 
TTTGACATTC CAAGAAGTGA CTGCATAAGG ATCTCTGCTA AATTTGGAAC AAATGTGGAG 
CGGGTTCTGC AAGAGGTTAT TACCAAAATT CCCCCGCCCA AGATGGAGAG AATGAGCCCC 
CTAAAAGCTT TACTGTTTGA CTCCACCTTT GATCACTACA GAGGAGTTGT GGCTAATGTT 
GCACTGTTTG GCGGGGAGGT AACCAAAGGC CACAAGATTC TCTCCGCACA CACGGGGAAG 
AGCTATGAAG TCAATGAAGT TGGTATCCTG ACTCCGGAAG AAGTGCCAGC AGATAAATTA 
TATGCTGGGC AGGTGGGATA CCTCATTGCA GGGATGAAAG AAGTAAAAGA AGCACAAATA 
GGAGACACTC TGTATCTGCA GAAACAGCCG GTAGAGCCCC TGCCTGGGTT CAAATCTGCC 
AAGCCCATGG TGTTTGCTGG CATGTATCCT GTTGACCAGT CTGAATACAA TAACTTGAAG 
GGGGCGTTAG AGAAACTGAC ATTAAATGAC TCTAGTGTCA CAGTGCATCG GGACAGCAGT 
GCTGCACTTG GGGCTGGTTG GAGGTTGGGA TTCCTTGGGT TGTTGCACCT GGAAGTATTT 
AACCAGCGCT TAGAGCAGGA ATACAATGCG TCAGTAATAA TGACTTCACC AACTGTTCCA 
TACAGGGCCA TCCTTTCTTC TCCCAAACTA ATTAAGGAGT ATGGAGAAAA GGAGATAACA 
ATAGTAAACC CTGCAGAATT CCCAGACAAG TCTCAGGTGT TGGAATATCT AGAGCCCATG 
GTATTGGGAA CAATCATTTC ACCGGACGAA TTCATGAGCA AAATCCTATC TCTGTGTCAG 
TCACGAAGAG GAATTCAGAA AAGCCTGGTG TATATCGACG ATCACAGAAT CATGATGAAA 
TATCTTTTTC CTTTGAATGA AATTGTAGTA GATTTTTACG ATGGACTAAA GTCTCTGTCA 
TCTGGATATG CAAGCTTTGA TTATGAAGAC GCAGGGTACC AGTCAGCTGA CCTAGTGAAA 
ATGGACATCC TGCTAAACGG GCGCCCCGTG GAAGAGCTGG TCACTATTGC CCACAGGGAA 
AAGGCCTACC CAGTTGGAAA GGCAATATGT GAAAGGCTGT GCGAGTCCAT CCCCAGGCAG 
TTGTTTGAAA TAGCCATCCA AGCTTCTATC GGTAGCAAAG TCATAGCAAG AGAAACAGTT 
AAAGCTTACA GAAAGAATGT CCTGGCAAAA TGCTACGGAG GTGATATCAC AAGGAAAATG 
AAGCTGCTGA AGAGGCAAGC TGAGGGAAAG AAGAAAATGA GAAAAATCGG CAATGTGGAA 
GTGCCAAAAG ATGCTTTTAT CAGTGTGTTG AAACGAAAAC CAGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

guf1 ( XM_004911213.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911270.1 Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X2, mRNA.

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CloneID	OXa04737
Clone ID Related Accession (Same CDS sequence)	XM_004911213.3 , XM_004911213.4 , XM_002933449.4 , XM_012955693.3 , XM_012955693.2 , XM_002933449.5
Accession Version	XM_004911213.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation factor GUF1, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911213.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCC TGTTTCCTCA TTCTACTTCA AAATGTCTGC GCTTGTGGAG ACACACGATT 
TCTGCCCAGC AGAGGCACCT GGCATCTCAG AACTTGTTCG GTACCACTCT GTTTGGCCTT 
CACCACTTGC AGAAAACTGT TAACCCTATG AATATTCTGA GTCCAAGGCA ATTTAGTTCT 
CAGAAGGAAA AACTTGACAT GAGTGAATAT GCAGTTGACT ATATAAGGAA TTTTTGCATA 
ATTGCCCATG TTGACCATGG TAAAAGTACT CTTGCTGACA GACTGCTTGA AATAACAGGG 
CGGATTCCAA AAACAGATTC AAATAAACAA GTTTTGGACA AGCTCCAGGT GGAGCGAGAG 
AGAGGAATTA CTGTCAAGGC ACAGACTGCG TCTCTCTTTT ATACTTTTGA AGGAAAAAAG 
TACCTCTTAA ACCTGATAGA CACACCAGGT CACGTGGATT TCAGCTATGA AGTATCTAGG 
TCCCTGTCTG CCTGTCAAGG TGTTTTGCTT GTGGTTGATG CCAATGAGGG TATTCAAGCA 
CAGACTGTGG CAAACTTCTT CCTAGCCTTT GAAGCACAGC TTTCAATTAT TCCCGTCATA 
AACAAGATTG ATTTGAAGAA TGCAGACCCT GAAAGAGCAG AAAAGCAAAT TGAAAAGATG 
TTTGACATTC CAAGAAGTGA CTGCATAAGG ATCTCTGCTA AATTTGGAAC AAATGTGGAG 
CGGGTTCTGC AAGAGGTTAT TACCAAAATT CCCCCGCCCA AGATGGAGAG AATGAGCCCC 
CTAAAAGCTT TACTGTTTGA CTCCACCTTT GATCACTACA GAGGAGTTGT GGCTAATGTT 
GCACTGTTTG GCGGGGAGGT AACCAAAGGC CACAAGATTC TCTCCGCACA CACGGGGAAG 
AGCTATGAAG TCAATGAAGT TGGTATCCTG ACTCCGGAAG AAGTGCCAGC AGATAAATTA 
TATGCTGGGC AGGTGGGATA CCTCATTGCA GGGATGAAAG AAGTAAAAGA AGCACAAATA 
GGAGACACTC TGTATCTGCA GAAACAGCCG GTAGAGCCCC TGCCTGGGTT CAAATCTGCC 
AAGCCCATGG TGTTTGCTGG CATGTATCCT GTTGACCAGT CTGAATACAA TAACTTGAAG 
GGGGCGTTAG AGAAACTGAC ATTAAATGAC TCTAGTGTCA CAGTGCATCG GGACAGCAGT 
GCTGCACTTG GGGCTGGTTG GAGGTTGGGA TTCCTTGGGT TGTTGCACCT GGAAGTATTT 
AACCAGCGCT TAGAGCAGGA ATACAATGCG TCAGTAATAA TGACTTCACC AACTGTTCCA 
TACAGGGCCA TCCTTTCTTC TCCCAAACTA ATTAAGGAGT ATGGAGAAAA GGAGATAACA 
ATAGTAAACC CTGCAGAATT CCCAGACAAG TCTCAGGTGT TGGAATATCT AGAGCCCATG 
GTATTGGGAA CAATCATTTC ACCGGACGAA TTCATGAGCA AAATCCTATC TCTGTGTCAG 
TCACGAAGAG GAATTCAGAA AAGCCTGGTG TATATCGACG ATCACAGAAT CATGATGAAA 
TATCTTTTTC CTTTGAATGA AATTGTAGTA GATTTTTACG ATGGACTAAA GTCTCTGTCA 
TCTGGATATG CAAGCTTTGA TTATGAAGAC GCAGGGTACC AGTCAGCTGA CCTAGTGAAA 
ATGGACATCC TGCTAAACGG GCGCCCCGTG GAAGAGCTGG TCACTATTGC CCACAGGGAA 
AAGGCCTACC CAGTTGGAAA GGCAATATGT GAAAGGCTGT GCGAGTCCAT CCCCAGGCAG 
TTGTTTGAAA TAGCCATCCA AGCTTCTATC GGTAGCAAAG TCATAGCAAG AGAAACAGTT 
AAAGCTTACA GAAAGAATGT CCTGGCAAAA TGCTACGGAG GTGATATCAC AAGGAAAATG 
AAGCTGCTGA AGAGGCAAGC TGAGGGAAAG AAGAAAATGA GAAAAATCGG CAATGTGGAA 
GTGCCAAAAG ATGCTTTTAT CAGTGTGTTG AAACGAAAAC CAGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911270.1
CDS	187..2217
Translation	MSSLFPHSTSKCLRLWRHTISAQQRHLASQNLFGTTLFGLHHLQKTVNPMNILSPRQFSSQKEKLDMSEYAVDYIRNFCIIAHVDHGKSTLADRLLEITGRIPKTDSNKQVLDKLQVERERGITVKAQTASLFYTFEGKKYLLNLIDTPGHVDFSYEVSRSLSACQGVLLVVDANEGIQAQTVANFFLAFEAQLSIIPVINKIDLKNADPERAEKQIEKMFDIPRSDCIRISAKFGTNVERVLQEVITKIPPPKMERMSPLKALLFDSTFDHYRGVVANVALFGGEVTKGHKILSAHTGKSYEVNEVGILTPEEVPADKLYAGQVGYLIAGMKEVKEAQIGDTLYLQKQPVEPLPGFKSAKPMVFAGMYPVDQSEYNNLKGALEKLTLNDSSVTVHRDSSAALGAGWRLGFLGLLHLEVFNQRLEQEYNASVIMTSPTVPYRAILSSPKLIKEYGEKEITIVNPAEFPDKSQVLEYLEPMVLGTIISPDEFMSKILSLCQSRRGIQKSLVYIDDHRIMMKYLFPLNEIVVDFYDGLKSLSSGYASFDYEDAGYQSADLVKMDILLNGRPVEELVTIAHREKAYPVGKAICERLCESIPRQLFEIAIQASIGSKVIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKKMRKIGNVEVPKDAFISVLKRKPDK

Target ORF information:

RefSeq Version	XM_004911213.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911213.4

ORF Insert Sequence:

ATGTCCTCCC TGTTTCCTCA TTCTACTTCA AAATGTCTGC GCTTGTGGAG ACACACGATT 
TCTGCCCAGC AGAGGCACCT GGCATCTCAG AACTTGTTCG GTACCACTCT GTTTGGCCTT 
CACCACTTGC AGAAAACTGT TAACCCTATG AATATTCTGA GTCCAAGGCA ATTTAGTTCT 
CAGAAGGAAA AACTTGACAT GAGTGAATAT GCAGTTGACT ATATAAGGAA TTTTTGCATA 
ATTGCCCATG TTGACCATGG TAAAAGTACT CTTGCTGACA GACTGCTTGA AATAACAGGG 
CGGATTCCAA AAACAGATTC AAATAAACAA GTTTTGGACA AGCTCCAGGT GGAGCGAGAG 
AGAGGAATTA CTGTCAAGGC ACAGACTGCG TCTCTCTTTT ATACTTTTGA AGGAAAAAAG 
TACCTCTTAA ACCTGATAGA CACACCAGGT CACGTGGATT TCAGCTATGA AGTATCTAGG 
TCCCTGTCTG CCTGTCAAGG TGTTTTGCTT GTGGTTGATG CCAATGAGGG TATTCAAGCA 
CAGACTGTGG CAAACTTCTT CCTAGCCTTT GAAGCACAGC TTTCAATTAT TCCCGTCATA 
AACAAGATTG ATTTGAAGAA TGCAGACCCT GAAAGAGCAG AAAAGCAAAT TGAAAAGATG 
TTTGACATTC CAAGAAGTGA CTGCATAAGG ATCTCTGCTA AATTTGGAAC AAATGTGGAG 
CGGGTTCTGC AAGAGGTTAT TACCAAAATT CCCCCGCCCA AGATGGAGAG AATGAGCCCC 
CTAAAAGCTT TACTGTTTGA CTCCACCTTT GATCACTACA GAGGAGTTGT GGCTAATGTT 
GCACTGTTTG GCGGGGAGGT AACCAAAGGC CACAAGATTC TCTCCGCACA CACGGGGAAG 
AGCTATGAAG TCAATGAAGT TGGTATCCTG ACTCCGGAAG AAGTGCCAGC AGATAAATTA 
TATGCTGGGC AGGTGGGATA CCTCATTGCA GGGATGAAAG AAGTAAAAGA AGCACAAATA 
GGAGACACTC TGTATCTGCA GAAACAGCCG GTAGAGCCCC TGCCTGGGTT CAAATCTGCC 
AAGCCCATGG TGTTTGCTGG CATGTATCCT GTTGACCAGT CTGAATACAA TAACTTGAAG 
GGGGCGTTAG AGAAACTGAC ATTAAATGAC TCTAGTGTCA CAGTGCATCG GGACAGCAGT 
GCTGCACTTG GGGCTGGTTG GAGGTTGGGA TTCCTTGGGT TGTTGCACCT GGAAGTATTT 
AACCAGCGCT TAGAGCAGGA ATACAATGCG TCAGTAATAA TGACTTCACC AACTGTTCCA 
TACAGGGCCA TCCTTTCTTC TCCCAAACTA ATTAAGGAGT ATGGAGAAAA GGAGATAACA 
ATAGTAAACC CTGCAGAATT CCCAGACAAG TCTCAGGTGT TGGAATATCT AGAGCCCATG 
GTATTGGGAA CAATCATTTC ACCGGACGAA TTCATGAGCA AAATCCTATC TCTGTGTCAG 
TCACGAAGAG GAATTCAGAA AAGCCTGGTG TATATCGACG ATCACAGAAT CATGATGAAA 
TATCTTTTTC CTTTGAATGA AATTGTAGTA GATTTTTACG ATGGACTAAA GTCTCTGTCA 
TCTGGATATG CAAGCTTTGA TTATGAAGAC GCAGGGTACC AGTCAGCTGA CCTAGTGAAA 
ATGGACATCC TGCTAAACGG GCGCCCCGTG GAAGAGCTGG TCACTATTGC CCACAGGGAA 
AAGGCCTACC CAGTTGGAAA GGCAATATGT GAAAGGCTGT GCGAGTCCAT CCCCAGGCAG 
TTGTTTGAAA TAGCCATCCA AGCTTCTATC GGTAGCAAAG TCATAGCAAG AGAAACAGTT 
AAAGCTTACA GAAAGAATGT CCTGGCAAAA TGCTACGGAG GTGATATCAC AAGGAAAATG 
AAGCTGCTGA AGAGGCAAGC TGAGGGAAAG AAGAAAATGA GAAAAATCGG CAATGTGGAA 
GTGCCAAAAG ATGCTTTTAT CAGTGTGTTG AAACGAAAAC CAGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

guf1 ( XM_012955693.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811147.1 Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa04737
Clone ID Related Accession (Same CDS sequence)	XM_004911213.3 , XM_004911213.4 , XM_002933449.4 , XM_012955693.3 , XM_012955693.2 , XM_002933449.5
Accession Version	XM_012955693.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation factor GUF1, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012955693.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCC TGTTTCCTCA TTCTACTTCA AAATGTCTGC GCTTGTGGAG ACACACGATT 
TCTGCCCAGC AGAGGCACCT GGCATCTCAG AACTTGTTCG GTACCACTCT GTTTGGCCTT 
CACCACTTGC AGAAAACTGT TAACCCTATG AATATTCTGA GTCCAAGGCA ATTTAGTTCT 
CAGAAGGAAA AACTTGACAT GAGTGAATAT GCAGTTGACT ATATAAGGAA TTTTTGCATA 
ATTGCCCATG TTGACCATGG TAAAAGTACT CTTGCTGACA GACTGCTTGA AATAACAGGG 
CGGATTCCAA AAACAGATTC AAATAAACAA GTTTTGGACA AGCTCCAGGT GGAGCGAGAG 
AGAGGAATTA CTGTCAAGGC ACAGACTGCG TCTCTCTTTT ATACTTTTGA AGGAAAAAAG 
TACCTCTTAA ACCTGATAGA CACACCAGGT CACGTGGATT TCAGCTATGA AGTATCTAGG 
TCCCTGTCTG CCTGTCAAGG TGTTTTGCTT GTGGTTGATG CCAATGAGGG TATTCAAGCA 
CAGACTGTGG CAAACTTCTT CCTAGCCTTT GAAGCACAGC TTTCAATTAT TCCCGTCATA 
AACAAGATTG ATTTGAAGAA TGCAGACCCT GAAAGAGCAG AAAAGCAAAT TGAAAAGATG 
TTTGACATTC CAAGAAGTGA CTGCATAAGG ATCTCTGCTA AATTTGGAAC AAATGTGGAG 
CGGGTTCTGC AAGAGGTTAT TACCAAAATT CCCCCGCCCA AGATGGAGAG AATGAGCCCC 
CTAAAAGCTT TACTGTTTGA CTCCACCTTT GATCACTACA GAGGAGTTGT GGCTAATGTT 
GCACTGTTTG GCGGGGAGGT AACCAAAGGC CACAAGATTC TCTCCGCACA CACGGGGAAG 
AGCTATGAAG TCAATGAAGT TGGTATCCTG ACTCCGGAAG AAGTGCCAGC AGATAAATTA 
TATGCTGGGC AGGTGGGATA CCTCATTGCA GGGATGAAAG AAGTAAAAGA AGCACAAATA 
GGAGACACTC TGTATCTGCA GAAACAGCCG GTAGAGCCCC TGCCTGGGTT CAAATCTGCC 
AAGCCCATGG TGTTTGCTGG CATGTATCCT GTTGACCAGT CTGAATACAA TAACTTGAAG 
GGGGCGTTAG AGAAACTGAC ATTAAATGAC TCTAGTGTCA CAGTGCATCG GGACAGCAGT 
GCTGCACTTG GGGCTGGTTG GAGGTTGGGA TTCCTTGGGT TGTTGCACCT GGAAGTATTT 
AACCAGCGCT TAGAGCAGGA ATACAATGCG TCAGTAATAA TGACTTCACC AACTGTTCCA 
TACAGGGCCA TCCTTTCTTC TCCCAAACTA ATTAAGGAGT ATGGAGAAAA GGAGATAACA 
ATAGTAAACC CTGCAGAATT CCCAGACAAG TCTCAGGTGT TGGAATATCT AGAGCCCATG 
GTATTGGGAA CAATCATTTC ACCGGACGAA TTCATGAGCA AAATCCTATC TCTGTGTCAG 
TCACGAAGAG GAATTCAGAA AAGCCTGGTG TATATCGACG ATCACAGAAT CATGATGAAA 
TATCTTTTTC CTTTGAATGA AATTGTAGTA GATTTTTACG ATGGACTAAA GTCTCTGTCA 
TCTGGATATG CAAGCTTTGA TTATGAAGAC GCAGGGTACC AGTCAGCTGA CCTAGTGAAA 
ATGGACATCC TGCTAAACGG GCGCCCCGTG GAAGAGCTGG TCACTATTGC CCACAGGGAA 
AAGGCCTACC CAGTTGGAAA GGCAATATGT GAAAGGCTGT GCGAGTCCAT CCCCAGGCAG 
TTGTTTGAAA TAGCCATCCA AGCTTCTATC GGTAGCAAAG TCATAGCAAG AGAAACAGTT 
AAAGCTTACA GAAAGAATGT CCTGGCAAAA TGCTACGGAG GTGATATCAC AAGGAAAATG 
AAGCTGCTGA AGAGGCAAGC TGAGGGAAAG AAGAAAATGA GAAAAATCGG CAATGTGGAA 
GTGCCAAAAG ATGCTTTTAT CAGTGTGTTG AAACGAAAAC CAGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811147.1
CDS	126..2156
Translation	MSSLFPHSTSKCLRLWRHTISAQQRHLASQNLFGTTLFGLHHLQKTVNPMNILSPRQFSSQKEKLDMSEYAVDYIRNFCIIAHVDHGKSTLADRLLEITGRIPKTDSNKQVLDKLQVERERGITVKAQTASLFYTFEGKKYLLNLIDTPGHVDFSYEVSRSLSACQGVLLVVDANEGIQAQTVANFFLAFEAQLSIIPVINKIDLKNADPERAEKQIEKMFDIPRSDCIRISAKFGTNVERVLQEVITKIPPPKMERMSPLKALLFDSTFDHYRGVVANVALFGGEVTKGHKILSAHTGKSYEVNEVGILTPEEVPADKLYAGQVGYLIAGMKEVKEAQIGDTLYLQKQPVEPLPGFKSAKPMVFAGMYPVDQSEYNNLKGALEKLTLNDSSVTVHRDSSAALGAGWRLGFLGLLHLEVFNQRLEQEYNASVIMTSPTVPYRAILSSPKLIKEYGEKEITIVNPAEFPDKSQVLEYLEPMVLGTIISPDEFMSKILSLCQSRRGIQKSLVYIDDHRIMMKYLFPLNEIVVDFYDGLKSLSSGYASFDYEDAGYQSADLVKMDILLNGRPVEELVTIAHREKAYPVGKAICERLCESIPRQLFEIAIQASIGSKVIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKKMRKIGNVEVPKDAFISVLKRKPDK

Target ORF information:

RefSeq Version	XM_012955693.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955693.3

ORF Insert Sequence:

ATGTCCTCCC TGTTTCCTCA TTCTACTTCA AAATGTCTGC GCTTGTGGAG ACACACGATT 
TCTGCCCAGC AGAGGCACCT GGCATCTCAG AACTTGTTCG GTACCACTCT GTTTGGCCTT 
CACCACTTGC AGAAAACTGT TAACCCTATG AATATTCTGA GTCCAAGGCA ATTTAGTTCT 
CAGAAGGAAA AACTTGACAT GAGTGAATAT GCAGTTGACT ATATAAGGAA TTTTTGCATA 
ATTGCCCATG TTGACCATGG TAAAAGTACT CTTGCTGACA GACTGCTTGA AATAACAGGG 
CGGATTCCAA AAACAGATTC AAATAAACAA GTTTTGGACA AGCTCCAGGT GGAGCGAGAG 
AGAGGAATTA CTGTCAAGGC ACAGACTGCG TCTCTCTTTT ATACTTTTGA AGGAAAAAAG 
TACCTCTTAA ACCTGATAGA CACACCAGGT CACGTGGATT TCAGCTATGA AGTATCTAGG 
TCCCTGTCTG CCTGTCAAGG TGTTTTGCTT GTGGTTGATG CCAATGAGGG TATTCAAGCA 
CAGACTGTGG CAAACTTCTT CCTAGCCTTT GAAGCACAGC TTTCAATTAT TCCCGTCATA 
AACAAGATTG ATTTGAAGAA TGCAGACCCT GAAAGAGCAG AAAAGCAAAT TGAAAAGATG 
TTTGACATTC CAAGAAGTGA CTGCATAAGG ATCTCTGCTA AATTTGGAAC AAATGTGGAG 
CGGGTTCTGC AAGAGGTTAT TACCAAAATT CCCCCGCCCA AGATGGAGAG AATGAGCCCC 
CTAAAAGCTT TACTGTTTGA CTCCACCTTT GATCACTACA GAGGAGTTGT GGCTAATGTT 
GCACTGTTTG GCGGGGAGGT AACCAAAGGC CACAAGATTC TCTCCGCACA CACGGGGAAG 
AGCTATGAAG TCAATGAAGT TGGTATCCTG ACTCCGGAAG AAGTGCCAGC AGATAAATTA 
TATGCTGGGC AGGTGGGATA CCTCATTGCA GGGATGAAAG AAGTAAAAGA AGCACAAATA 
GGAGACACTC TGTATCTGCA GAAACAGCCG GTAGAGCCCC TGCCTGGGTT CAAATCTGCC 
AAGCCCATGG TGTTTGCTGG CATGTATCCT GTTGACCAGT CTGAATACAA TAACTTGAAG 
GGGGCGTTAG AGAAACTGAC ATTAAATGAC TCTAGTGTCA CAGTGCATCG GGACAGCAGT 
GCTGCACTTG GGGCTGGTTG GAGGTTGGGA TTCCTTGGGT TGTTGCACCT GGAAGTATTT 
AACCAGCGCT TAGAGCAGGA ATACAATGCG TCAGTAATAA TGACTTCACC AACTGTTCCA 
TACAGGGCCA TCCTTTCTTC TCCCAAACTA ATTAAGGAGT ATGGAGAAAA GGAGATAACA 
ATAGTAAACC CTGCAGAATT CCCAGACAAG TCTCAGGTGT TGGAATATCT AGAGCCCATG 
GTATTGGGAA CAATCATTTC ACCGGACGAA TTCATGAGCA AAATCCTATC TCTGTGTCAG 
TCACGAAGAG GAATTCAGAA AAGCCTGGTG TATATCGACG ATCACAGAAT CATGATGAAA 
TATCTTTTTC CTTTGAATGA AATTGTAGTA GATTTTTACG ATGGACTAAA GTCTCTGTCA 
TCTGGATATG CAAGCTTTGA TTATGAAGAC GCAGGGTACC AGTCAGCTGA CCTAGTGAAA 
ATGGACATCC TGCTAAACGG GCGCCCCGTG GAAGAGCTGG TCACTATTGC CCACAGGGAA 
AAGGCCTACC CAGTTGGAAA GGCAATATGT GAAAGGCTGT GCGAGTCCAT CCCCAGGCAG 
TTGTTTGAAA TAGCCATCCA AGCTTCTATC GGTAGCAAAG TCATAGCAAG AGAAACAGTT 
AAAGCTTACA GAAAGAATGT CCTGGCAAAA TGCTACGGAG GTGATATCAC AAGGAAAATG 
AAGCTGCTGA AGAGGCAAGC TGAGGGAAAG AAGAAAATGA GAAAAATCGG CAATGTGGAA 
GTGCCAAAAG ATGCTTTTAT CAGTGTGTTG AAACGAAAAC CAGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

guf1 ( XM_002933449.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933495.1 Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X1, mRNA.

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CloneID	OXa04737
Clone ID Related Accession (Same CDS sequence)	XM_004911213.3 , XM_004911213.4 , XM_002933449.4 , XM_012955693.3 , XM_012955693.2 , XM_002933449.5
Accession Version	XM_002933449.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	translation factor GUF1, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933449.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCCC TGTTTCCTCA TTCTACTTCA AAATGTCTGC GCTTGTGGAG ACACACGATT 
TCTGCCCAGC AGAGGCACCT GGCATCTCAG AACTTGTTCG GTACCACTCT GTTTGGCCTT 
CACCACTTGC AGAAAACTGT TAACCCTATG AATATTCTGA GTCCAAGGCA ATTTAGTTCT 
CAGAAGGAAA AACTTGACAT GAGTGAATAT GCAGTTGACT ATATAAGGAA TTTTTGCATA 
ATTGCCCATG TTGACCATGG TAAAAGTACT CTTGCTGACA GACTGCTTGA AATAACAGGG 
CGGATTCCAA AAACAGATTC AAATAAACAA GTTTTGGACA AGCTCCAGGT GGAGCGAGAG 
AGAGGAATTA CTGTCAAGGC ACAGACTGCG TCTCTCTTTT ATACTTTTGA AGGAAAAAAG 
TACCTCTTAA ACCTGATAGA CACACCAGGT CACGTGGATT TCAGCTATGA AGTATCTAGG 
TCCCTGTCTG CCTGTCAAGG TGTTTTGCTT GTGGTTGATG CCAATGAGGG TATTCAAGCA 
CAGACTGTGG CAAACTTCTT CCTAGCCTTT GAAGCACAGC TTTCAATTAT TCCCGTCATA 
AACAAGATTG ATTTGAAGAA TGCAGACCCT GAAAGAGCAG AAAAGCAAAT TGAAAAGATG 
TTTGACATTC CAAGAAGTGA CTGCATAAGG ATCTCTGCTA AATTTGGAAC AAATGTGGAG 
CGGGTTCTGC AAGAGGTTAT TACCAAAATT CCCCCGCCCA AGATGGAGAG AATGAGCCCC 
CTAAAAGCTT TACTGTTTGA CTCCACCTTT GATCACTACA GAGGAGTTGT GGCTAATGTT 
GCACTGTTTG GCGGGGAGGT AACCAAAGGC CACAAGATTC TCTCCGCACA CACGGGGAAG 
AGCTATGAAG TCAATGAAGT TGGTATCCTG ACTCCGGAAG AAGTGCCAGC AGATAAATTA 
TATGCTGGGC AGGTGGGATA CCTCATTGCA GGGATGAAAG AAGTAAAAGA AGCACAAATA 
GGAGACACTC TGTATCTGCA GAAACAGCCG GTAGAGCCCC TGCCTGGGTT CAAATCTGCC 
AAGCCCATGG TGTTTGCTGG CATGTATCCT GTTGACCAGT CTGAATACAA TAACTTGAAG 
GGGGCGTTAG AGAAACTGAC ATTAAATGAC TCTAGTGTCA CAGTGCATCG GGACAGCAGT 
GCTGCACTTG GGGCTGGTTG GAGGTTGGGA TTCCTTGGGT TGTTGCACCT GGAAGTATTT 
AACCAGCGCT TAGAGCAGGA ATACAATGCG TCAGTAATAA TGACTTCACC AACTGTTCCA 
TACAGGGCCA TCCTTTCTTC TCCCAAACTA ATTAAGGAGT ATGGAGAAAA GGAGATAACA 
ATAGTAAACC CTGCAGAATT CCCAGACAAG TCTCAGGTGT TGGAATATCT AGAGCCCATG 
GTATTGGGAA CAATCATTTC ACCGGACGAA TTCATGAGCA AAATCCTATC TCTGTGTCAG 
TCACGAAGAG GAATTCAGAA AAGCCTGGTG TATATCGACG ATCACAGAAT CATGATGAAA 
TATCTTTTTC CTTTGAATGA AATTGTAGTA GATTTTTACG ATGGACTAAA GTCTCTGTCA 
TCTGGATATG CAAGCTTTGA TTATGAAGAC GCAGGGTACC AGTCAGCTGA CCTAGTGAAA 
ATGGACATCC TGCTAAACGG GCGCCCCGTG GAAGAGCTGG TCACTATTGC CCACAGGGAA 
AAGGCCTACC CAGTTGGAAA GGCAATATGT GAAAGGCTGT GCGAGTCCAT CCCCAGGCAG 
TTGTTTGAAA TAGCCATCCA AGCTTCTATC GGTAGCAAAG TCATAGCAAG AGAAACAGTT 
AAAGCTTACA GAAAGAATGT CCTGGCAAAA TGCTACGGAG GTGATATCAC AAGGAAAATG 
AAGCTGCTGA AGAGGCAAGC TGAGGGAAAG AAGAAAATGA GAAAAATCGG CAATGTGGAA 
GTGCCAAAAG ATGCTTTTAT CAGTGTGTTG AAACGAAAAC CAGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933495.1
CDS	294..2324
Translation	MSSLFPHSTSKCLRLWRHTISAQQRHLASQNLFGTTLFGLHHLQKTVNPMNILSPRQFSSQKEKLDMSEYAVDYIRNFCIIAHVDHGKSTLADRLLEITGRIPKTDSNKQVLDKLQVERERGITVKAQTASLFYTFEGKKYLLNLIDTPGHVDFSYEVSRSLSACQGVLLVVDANEGIQAQTVANFFLAFEAQLSIIPVINKIDLKNADPERAEKQIEKMFDIPRSDCIRISAKFGTNVERVLQEVITKIPPPKMERMSPLKALLFDSTFDHYRGVVANVALFGGEVTKGHKILSAHTGKSYEVNEVGILTPEEVPADKLYAGQVGYLIAGMKEVKEAQIGDTLYLQKQPVEPLPGFKSAKPMVFAGMYPVDQSEYNNLKGALEKLTLNDSSVTVHRDSSAALGAGWRLGFLGLLHLEVFNQRLEQEYNASVIMTSPTVPYRAILSSPKLIKEYGEKEITIVNPAEFPDKSQVLEYLEPMVLGTIISPDEFMSKILSLCQSRRGIQKSLVYIDDHRIMMKYLFPLNEIVVDFYDGLKSLSSGYASFDYEDAGYQSADLVKMDILLNGRPVEELVTIAHREKAYPVGKAICERLCESIPRQLFEIAIQASIGSKVIARETVKAYRKNVLAKCYGGDITRKMKLLKRQAEGKKKMRKIGNVEVPKDAFISVLKRKPDK

Target ORF information:

RefSeq Version	XM_002933449.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis GUF1 homolog, GTPase (guf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933449.5

ORF Insert Sequence:

ATGTCCTCCC TGTTTCCTCA TTCTACTTCA AAATGTCTGC GCTTGTGGAG ACACACGATT 
TCTGCCCAGC AGAGGCACCT GGCATCTCAG AACTTGTTCG GTACCACTCT GTTTGGCCTT 
CACCACTTGC AGAAAACTGT TAACCCTATG AATATTCTGA GTCCAAGGCA ATTTAGTTCT 
CAGAAGGAAA AACTTGACAT GAGTGAATAT GCAGTTGACT ATATAAGGAA TTTTTGCATA 
ATTGCCCATG TTGACCATGG TAAAAGTACT CTTGCTGACA GACTGCTTGA AATAACAGGG 
CGGATTCCAA AAACAGATTC AAATAAACAA GTTTTGGACA AGCTCCAGGT GGAGCGAGAG 
AGAGGAATTA CTGTCAAGGC ACAGACTGCG TCTCTCTTTT ATACTTTTGA AGGAAAAAAG 
TACCTCTTAA ACCTGATAGA CACACCAGGT CACGTGGATT TCAGCTATGA AGTATCTAGG 
TCCCTGTCTG CCTGTCAAGG TGTTTTGCTT GTGGTTGATG CCAATGAGGG TATTCAAGCA 
CAGACTGTGG CAAACTTCTT CCTAGCCTTT GAAGCACAGC TTTCAATTAT TCCCGTCATA 
AACAAGATTG ATTTGAAGAA TGCAGACCCT GAAAGAGCAG AAAAGCAAAT TGAAAAGATG 
TTTGACATTC CAAGAAGTGA CTGCATAAGG ATCTCTGCTA AATTTGGAAC AAATGTGGAG 
CGGGTTCTGC AAGAGGTTAT TACCAAAATT CCCCCGCCCA AGATGGAGAG AATGAGCCCC 
CTAAAAGCTT TACTGTTTGA CTCCACCTTT GATCACTACA GAGGAGTTGT GGCTAATGTT 
GCACTGTTTG GCGGGGAGGT AACCAAAGGC CACAAGATTC TCTCCGCACA CACGGGGAAG 
AGCTATGAAG TCAATGAAGT TGGTATCCTG ACTCCGGAAG AAGTGCCAGC AGATAAATTA 
TATGCTGGGC AGGTGGGATA CCTCATTGCA GGGATGAAAG AAGTAAAAGA AGCACAAATA 
GGAGACACTC TGTATCTGCA GAAACAGCCG GTAGAGCCCC TGCCTGGGTT CAAATCTGCC 
AAGCCCATGG TGTTTGCTGG CATGTATCCT GTTGACCAGT CTGAATACAA TAACTTGAAG 
GGGGCGTTAG AGAAACTGAC ATTAAATGAC TCTAGTGTCA CAGTGCATCG GGACAGCAGT 
GCTGCACTTG GGGCTGGTTG GAGGTTGGGA TTCCTTGGGT TGTTGCACCT GGAAGTATTT 
AACCAGCGCT TAGAGCAGGA ATACAATGCG TCAGTAATAA TGACTTCACC AACTGTTCCA 
TACAGGGCCA TCCTTTCTTC TCCCAAACTA ATTAAGGAGT ATGGAGAAAA GGAGATAACA 
ATAGTAAACC CTGCAGAATT CCCAGACAAG TCTCAGGTGT TGGAATATCT AGAGCCCATG 
GTATTGGGAA CAATCATTTC ACCGGACGAA TTCATGAGCA AAATCCTATC TCTGTGTCAG 
TCACGAAGAG GAATTCAGAA AAGCCTGGTG TATATCGACG ATCACAGAAT CATGATGAAA 
TATCTTTTTC CTTTGAATGA AATTGTAGTA GATTTTTACG ATGGACTAAA GTCTCTGTCA 
TCTGGATATG CAAGCTTTGA TTATGAAGAC GCAGGGTACC AGTCAGCTGA CCTAGTGAAA 
ATGGACATCC TGCTAAACGG GCGCCCCGTG GAAGAGCTGG TCACTATTGC CCACAGGGAA 
AAGGCCTACC CAGTTGGAAA GGCAATATGT GAAAGGCTGT GCGAGTCCAT CCCCAGGCAG 
TTGTTTGAAA TAGCCATCCA AGCTTCTATC GGTAGCAAAG TCATAGCAAG AGAAACAGTT 
AAAGCTTACA GAAAGAATGT CCTGGCAAAA TGCTACGGAG GTGATATCAC AAGGAAAATG 
AAGCTGCTGA AGAGGCAAGC TGAGGGAAAG AAGAAAATGA GAAAAATCGG CAATGTGGAA 
GTGCCAAAAG ATGCTTTTAT CAGTGTGTTG AAACGAAAAC CAGACAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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guf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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