syne3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following syne3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the syne3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa46400 XM_018096766.1
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa46401 XM_018096767.1
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OXa63894 XM_004917083.3
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa63894 XM_031890678.1
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa63894 XM_004917084.3
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa63895 XM_031890679.1
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa63896 XM_031890680.1
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa63897 XM_031890681.1
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa63898 XM_018096767.2
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa14725 XM_004917084.2
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa14725 XM_004917083.2
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa14727 XM_002936067.4
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa14728 XM_004917086.3
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa34169 XM_012969379.2
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa34170 XM_012969380.2
Latest version!
Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa46400
    Clone ID Related Accession (Same CDS sequence) XM_018096766.1
    Accession Version XM_018096766.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAGC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GTGCCTCTGA CCGCAGTAAC TCAGGTAGTG GTCAAACACT TCTAGGGAAC
    AAGCAGGCAT CCAGTGTTCT TCAAGTTGCT GATCCACTTA TCCCTTCAAC AGAGGCTGGG
    GAAAGTTCAA GAACTTATAC TGGAAGTATA GAAGAAAATT ATTTAGTGAG CACACCAGAA
    GAGTTTGGAG AGAGTTCAAG CTCTTTGACG ATAACTACTG GATCCCTGGC AGACAACCTG
    AAGAACCCAG TGAAACCAGA TTCTCAGTAC AATATGGTTT CTAGGAGAAG AAATAAGAAA
    GGTGTCAGCC TGGCAGAATC TGTGGATTTT GTGGATTCGT CTTATGTGCA AAGTAAAGAA
    GAGACAAAGA CAGAGAAAGG TGATGGTCAC ATGAATCTGC TGAAGGAATT TGAGCTGTGG
    TTGCAGGAAG AGAATTCCAA ACTAAACAAA ATCTGTAGCA CAAAAGCATC TAGCAGCGAG
    GAAATGAAAT CCAGGCTCAG TAAACTGCAG AGATTACAGT TGCAAGTACC CCGGGGTCAG
    CGCCTGTTTG AATCTTTGCT TGTTTCAATG CCAGACATGA CAGTTACTGA TAATTTAAGG
    CTGGAAGACC TGCGCTACCA ATGGATGCTT TATAAATCCA AATTAAGAGA TGCTGGAAGC
    TCATCGGGCA TAAATACATC TGAAGAGCTA AGAGGAATAA CAAAGAAGTC ATCTGGGGGA
    CTGTGCTCCT TCCTGCACCG TGTGTGCTGT GCCGCACTTC CACTCCAGTT ACTCCTTCTA
    TTACTTCTCC TCTTGGCATT GCTTATTCTT CTGATGCAGG AGAGCCAAAA CTGCGCCCTT
    TCCAACAACT TTGCCCGCTC CTTCAACCTG ATGCTAAAAT ACGAAAAACC TCCTCCAACT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952255.1
    CDS363..4145
    Translation

    Target ORF information:

    RefSeq Version XM_018096766.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAGC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GTGCCTCTGA CCGCAGTAAC TCAGGTAGTG GTCAAACACT TCTAGGGAAC
    AAGCAGGCAT CCAGTGTTCT TCAAGTTGCT GATCCACTTA TCCCTTCAAC AGAGGCTGGG
    GAAAGTTCAA GAACTTATAC TGGAAGTATA GAAGAAAATT ATTTAGTGAG CACACCAGAA
    GAGTTTGGAG AGAGTTCAAG CTCTTTGACG ATAACTACTG GATCCCTGGC AGACAACCTG
    AAGAACCCAG TGAAACCAGA TTCTCAGTAC AATATGGTTT CTAGGAGAAG AAATAAGAAA
    GGTGTCAGCC TGGCAGAATC TGTGGATTTT GTGGATTCGT CTTATGTGCA AAGTAAAGAA
    GAGACAAAGA CAGAGAAAGG TGATGGTCAC ATGAATCTGC TGAAGGAATT TGAGCTGTGG
    TTGCAGGAAG AGAATTCCAA ACTAAACAAA ATCTGTAGCA CAAAAGCATC TAGCAGCGAG
    GAAATGAAAT CCAGGCTCAG TAAACTGCAG AGATTACAGT TGCAAGTACC CCGGGGTCAG
    CGCCTGTTTG AATCTTTGCT TGTTTCAATG CCAGACATGA CAGTTACTGA TAATTTAAGG
    CTGGAAGACC TGCGCTACCA ATGGATGCTT TATAAATCCA AATTAAGAGA TGCTGGAAGC
    TCATCGGGCA TAAATACATC TGAAGAGCTA AGAGGAATAA CAAAGAAGTC ATCTGGGGGA
    CTGTGCTCCT TCCTGCACCG TGTGTGCTGT GCCGCACTTC CACTCCAGTT ACTCCTTCTA
    TTACTTCTCC TCTTGGCATT GCTTATTCTT CTGATGCAGG AGAGCCAAAA CTGCGCCCTT
    TCCAACAACT TTGCCCGCTC CTTCAACCTG ATGCTAAAAT ACGAAAAACC TCCTCCAACT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa46401
    Clone ID Related Accession (Same CDS sequence) XM_018096767.1
    Accession Version XM_018096767.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATAACAC TGAAGGGCCC AGATCTGCCC TGGAGGCCAG GCTCAGGGAG ACAGAGTCGC 
    ATTGAGTGGG TTTGGCTGCA CTGGAACGAG TACCTGAAAG CCAGAGATGA GTTTTATCTG
    TGGGTCCATA ACATGAAACT GACCCTGGAA CCAGATGTGG AACTTCAGCT GGGCGTTAAA
    GAGAAGCGCT GGCAATATGA ACAAGCACAA GTGCTGCTAA GAGATGTCAA CAACCAGTCA
    TGCCTGTTGG ACAGACTACT GGAGGAAGCC GCCTCTCTAT ACAACAGGAT TGGAGACCCC
    AGTGTGGATG AAACAGTGCA GAGAGAAATG ATGGCTGAAT ACAAACAAAT AAAGAAGAAA
    GCCAAGGAAA GGGCAAGTCA TCTTGAAAAT ATGTTGAAAG AACATGAAGC TTATGATAGG
    GATGTCAATG AATTCCGGTC TTGGCTGACC AGTGTGATTG AAAAGCTGAA GTGTTGTGTT
    GGAGAGGCCA TGGAATCAAC AGAATACAGG CTCAGTATGC TGCAGGAAAT CAGCAAAGAG
    GTACACAGTG GTGGTAAAAA GTTGGAAGAC CTTGAGGGCA AGTCACTTGA AGTGATAAAG
    AACACATCCC CTCTAGGTGC GGAGAATATC TGCAAGGAGC TGGAGGAGTT GCACAAAGCT
    CTAGCAGATC TGAAACGCAT GAATGATGAT GAAGAACAGA GTCTTCTCAA GGCTCATAAT
    TCTGAAAATG CCTTCCTACT TTTGGCTAGG CAACTAGAAG CCAACATCAA TGAGTTCCGA
    AAAGCCATCC AGAGACTGGA GGAAAGTCTG GAATCAGGAG AAAGGGTGAA GAGTGAAGAT
    GAACTGGTAG CTCTGTGGAA AACATTAAAC GCCACCAAGT CTGCTTTAGC AGCAGAGGAA
    ACAAAAGGTG AAGGGGTAAA GGTTCAGCTG AAGGAACTCT TCAAGTTCTC CAAGGATGTC
    CAGCCGCTTT CTGATGAAGT CATTGCTGCA ATTCGAGAAT ACCAAAGGGC TAAAAGCAAA
    GCCTTCAAGC TGAGTACTGA GACTGAATCG GCACTCATAC AGCACTTCCA GATTCCGCTT
    CTGGAATTTC ACCATTGGAA ACGCATAGGT GACAGAGTCC TGGATACCAC ATCAACTCCT
    GTGAGCGATT ATGCTCTGAA CCATGACTGC TTGCTACAGA TTGAGAGGTT GCTGGAGGAG
    AGTGCTAGTA TTGAAGAAAA ACTGGGTATT CTGCAGACAA AGAAGGAGCG AATCAGTGGG
    GTGCTTGGTG AACAGAAGGC AGAAACCTTT TTTACTGAGG TGGCATTTGC TTCAAGGGAA
    AGGGATTCTC TCCATGCTGT TCTATTGGAG AAAAAGAAAA TTTTACTGAG TTTGGCTTCC
    CAGAAGAAAG AATTTGATGC TGCTTTTGAA CAGTTGCGGA AGAAGGTGTT GACTTTAAGA
    ATTAAAGCTG GCAAAGAAAG TGAATTACAG CCAGACCTTG TAGGGAAAGA AACACAACTG
    CAGAGATTTC AGATGTTTAA GGATGATTTG TTAAAACTGG AACCTGAATT TAAAGAGGTA
    GGGTCAATGG CTGAGTCAAG TCCCACCCTC AAGCTTAAAG TCAGCCAGCT GTCTTCAGAA
    TACCTTACTT TACACAAGTC ACTTGAGATT AAAATACGAA AAAGCAAAGC ATACATTAAT
    GACCATCGGA TATTTAATGA GAAGCTGCTA GAGCTTCAGC GCTGGCTTAT GGTGACAAAG
    CAGGAGTTGG AATCATTTCA GGATAGTGAT GGGCATTGGG ATTTTGAATC CCGAGAGAAG
    GAAATACAGA TATTGTTGTC TGAGGTACCA GAAAAAGATA TCCAGCTACA ACAGGTAGAA
    TCTCAAGGTT TGCTAGTGAT GGAGACCTCC TCTCCAGAAG GAGCTGCCCA CATTCAGACT
    GAGCTGAGGG AGCTTAATGC CTCCTGGCTA AATCTGAAAC TACTCTGGGG GGCACTGGCA
    AGCGCGTTTA AAGACAGAGA GTTCCAGAGA CTGGCACTAC TGCCACCTAA AAGTACATCT
    GAGGACCACC TACATCAGAC AACTGACCTC CTCCTGAATA TGGAACAATC CTGGACTTCA
    TCTCAGTCTG ATAATAGCAA CAATGGCATG AACAACAGTA ATGATTCTGA AGAATTTGGA
    CCAAGTGGAA TTGTGGGCAG AATTGTCTAT GACACCAGTC CCCAAAGCTT AACATTAAGA
    TCAGAGAAAA GTCGGATACC ACAAAGGATA AACAAAACAA CCAGTTTGCT CCAAGAAAAG
    GATCCATCCT CTACAACAGC CTCTGATAAG AGTTTTGTCT CTCTGGAAGT AAATACTAGC
    ATGGCCGGTG ACTCCAGTCC ATCCAGGATA ACTGTGTGGT CAGACAAAAG CCGAATACCA
    ATGAGGATAA ACCAAACCTC CAGTTCCAGT GTTCTGCCAG AAAACTGTTC ACCCTTCACA
    CCACAAGAGT CTGGGATGAG TCCAAGAACT GTGAAGAGTG GACTTTTGAA TGACACCACG
    CCTCCCGATG TGGCAGTCAG ATCAGACACT AGTCAGCCGC AAAAAAGGAG AACGAAAGCA
    TCTAGGTTAG AGAGCCCACA TCCAACTATA TATTTTGATG CACATTTCTG CAGTGCCTCT
    GACCGCAGTA ACTCAGGTAG TGGTCAAACA CTTCTAGGGA ACAAGCAGGC ATCCAGTGTT
    CTTCAAGTTG CTGATCCACT TATCCCTTCA ACAGAGGCTG GGGAAAGTTC AAGAACTTAT
    ACTGGAAGTA TAGAAGAAAA TTATTTAGTG AGCACACCAG AAGAGTTTGG AGAGAGTTCA
    AGCTCTTTGA CGATAACTAC TGGATCCCTG GCAGACAACC TGAAGAACCC AGTGAAACCA
    GATTCTCAGT ACAATATGGT TTCTAGGAGA AGAAATAAGA AAGGTGTCAG CCTGGCAGAA
    TCTGTGGATT TTGTGGATTC GTCTTATGTG CAAAGTAAAG AAGAGACAAA GACAGAGAAA
    GGTGATGGTC ACATGAATCT GCTGAAGGAA TTTGAGCTGT GGTTGCAGGA AGAGAATTCC
    AAACTAAACA AAATCTGTAG CACAAAAGCA TCTAGCAGCG AGGAAATGAA ATCCAGGCTC
    AGTAAACTGC AGAGATTACA GTTGCAAGTA CCCCGGGGTC AGCGCCTGTT TGAATCTTTG
    CTTGTTTCAA TGCCAGACAT GACAGTTACT GATAATTTAA GGCTGGAAGA CCTGCGCTAC
    CAATGGATGC TTTATAAATC CAAATTAAGA GATGCTGGAA GCTCATCGGG CATAAATACA
    TCTGAAGAGC TAAGAGGAAT AACAAAGAAG TCATCTGGGG GACTGTGCTC CTTCCTGCAC
    CGTGTGTGCT GTGCCGCACT TCCACTCCAG TTACTCCTTC TATTACTTCT CCTCTTGGCA
    TTGCTTATTC TTCTGATGCA GGAGAGCCAA AACTGCGCCC TTTCCAACAA CTTTGCCCGC
    TCCTTCAACC TGATGCTAAA ATACGAAAAA CCTCCTCCAA CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952256.1
    CDS85..3609
    Translation

    Target ORF information:

    RefSeq Version XM_018096767.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATAACAC TGAAGGGCCC AGATCTGCCC TGGAGGCCAG GCTCAGGGAG ACAGAGTCGC 
    ATTGAGTGGG TTTGGCTGCA CTGGAACGAG TACCTGAAAG CCAGAGATGA GTTTTATCTG
    TGGGTCCATA ACATGAAACT GACCCTGGAA CCAGATGTGG AACTTCAGCT GGGCGTTAAA
    GAGAAGCGCT GGCAATATGA ACAAGCACAA GTGCTGCTAA GAGATGTCAA CAACCAGTCA
    TGCCTGTTGG ACAGACTACT GGAGGAAGCC GCCTCTCTAT ACAACAGGAT TGGAGACCCC
    AGTGTGGATG AAACAGTGCA GAGAGAAATG ATGGCTGAAT ACAAACAAAT AAAGAAGAAA
    GCCAAGGAAA GGGCAAGTCA TCTTGAAAAT ATGTTGAAAG AACATGAAGC TTATGATAGG
    GATGTCAATG AATTCCGGTC TTGGCTGACC AGTGTGATTG AAAAGCTGAA GTGTTGTGTT
    GGAGAGGCCA TGGAATCAAC AGAATACAGG CTCAGTATGC TGCAGGAAAT CAGCAAAGAG
    GTACACAGTG GTGGTAAAAA GTTGGAAGAC CTTGAGGGCA AGTCACTTGA AGTGATAAAG
    AACACATCCC CTCTAGGTGC GGAGAATATC TGCAAGGAGC TGGAGGAGTT GCACAAAGCT
    CTAGCAGATC TGAAACGCAT GAATGATGAT GAAGAACAGA GTCTTCTCAA GGCTCATAAT
    TCTGAAAATG CCTTCCTACT TTTGGCTAGG CAACTAGAAG CCAACATCAA TGAGTTCCGA
    AAAGCCATCC AGAGACTGGA GGAAAGTCTG GAATCAGGAG AAAGGGTGAA GAGTGAAGAT
    GAACTGGTAG CTCTGTGGAA AACATTAAAC GCCACCAAGT CTGCTTTAGC AGCAGAGGAA
    ACAAAAGGTG AAGGGGTAAA GGTTCAGCTG AAGGAACTCT TCAAGTTCTC CAAGGATGTC
    CAGCCGCTTT CTGATGAAGT CATTGCTGCA ATTCGAGAAT ACCAAAGGGC TAAAAGCAAA
    GCCTTCAAGC TGAGTACTGA GACTGAATCG GCACTCATAC AGCACTTCCA GATTCCGCTT
    CTGGAATTTC ACCATTGGAA ACGCATAGGT GACAGAGTCC TGGATACCAC ATCAACTCCT
    GTGAGCGATT ATGCTCTGAA CCATGACTGC TTGCTACAGA TTGAGAGGTT GCTGGAGGAG
    AGTGCTAGTA TTGAAGAAAA ACTGGGTATT CTGCAGACAA AGAAGGAGCG AATCAGTGGG
    GTGCTTGGTG AACAGAAGGC AGAAACCTTT TTTACTGAGG TGGCATTTGC TTCAAGGGAA
    AGGGATTCTC TCCATGCTGT TCTATTGGAG AAAAAGAAAA TTTTACTGAG TTTGGCTTCC
    CAGAAGAAAG AATTTGATGC TGCTTTTGAA CAGTTGCGGA AGAAGGTGTT GACTTTAAGA
    ATTAAAGCTG GCAAAGAAAG TGAATTACAG CCAGACCTTG TAGGGAAAGA AACACAACTG
    CAGAGATTTC AGATGTTTAA GGATGATTTG TTAAAACTGG AACCTGAATT TAAAGAGGTA
    GGGTCAATGG CTGAGTCAAG TCCCACCCTC AAGCTTAAAG TCAGCCAGCT GTCTTCAGAA
    TACCTTACTT TACACAAGTC ACTTGAGATT AAAATACGAA AAAGCAAAGC ATACATTAAT
    GACCATCGGA TATTTAATGA GAAGCTGCTA GAGCTTCAGC GCTGGCTTAT GGTGACAAAG
    CAGGAGTTGG AATCATTTCA GGATAGTGAT GGGCATTGGG ATTTTGAATC CCGAGAGAAG
    GAAATACAGA TATTGTTGTC TGAGGTACCA GAAAAAGATA TCCAGCTACA ACAGGTAGAA
    TCTCAAGGTT TGCTAGTGAT GGAGACCTCC TCTCCAGAAG GAGCTGCCCA CATTCAGACT
    GAGCTGAGGG AGCTTAATGC CTCCTGGCTA AATCTGAAAC TACTCTGGGG GGCACTGGCA
    AGCGCGTTTA AAGACAGAGA GTTCCAGAGA CTGGCACTAC TGCCACCTAA AAGTACATCT
    GAGGACCACC TACATCAGAC AACTGACCTC CTCCTGAATA TGGAACAATC CTGGACTTCA
    TCTCAGTCTG ATAATAGCAA CAATGGCATG AACAACAGTA ATGATTCTGA AGAATTTGGA
    CCAAGTGGAA TTGTGGGCAG AATTGTCTAT GACACCAGTC CCCAAAGCTT AACATTAAGA
    TCAGAGAAAA GTCGGATACC ACAAAGGATA AACAAAACAA CCAGTTTGCT CCAAGAAAAG
    GATCCATCCT CTACAACAGC CTCTGATAAG AGTTTTGTCT CTCTGGAAGT AAATACTAGC
    ATGGCCGGTG ACTCCAGTCC ATCCAGGATA ACTGTGTGGT CAGACAAAAG CCGAATACCA
    ATGAGGATAA ACCAAACCTC CAGTTCCAGT GTTCTGCCAG AAAACTGTTC ACCCTTCACA
    CCACAAGAGT CTGGGATGAG TCCAAGAACT GTGAAGAGTG GACTTTTGAA TGACACCACG
    CCTCCCGATG TGGCAGTCAG ATCAGACACT AGTCAGCCGC AAAAAAGGAG AACGAAAGCA
    TCTAGGTTAG AGAGCCCACA TCCAACTATA TATTTTGATG CACATTTCTG CAGTGCCTCT
    GACCGCAGTA ACTCAGGTAG TGGTCAAACA CTTCTAGGGA ACAAGCAGGC ATCCAGTGTT
    CTTCAAGTTG CTGATCCACT TATCCCTTCA ACAGAGGCTG GGGAAAGTTC AAGAACTTAT
    ACTGGAAGTA TAGAAGAAAA TTATTTAGTG AGCACACCAG AAGAGTTTGG AGAGAGTTCA
    AGCTCTTTGA CGATAACTAC TGGATCCCTG GCAGACAACC TGAAGAACCC AGTGAAACCA
    GATTCTCAGT ACAATATGGT TTCTAGGAGA AGAAATAAGA AAGGTGTCAG CCTGGCAGAA
    TCTGTGGATT TTGTGGATTC GTCTTATGTG CAAAGTAAAG AAGAGACAAA GACAGAGAAA
    GGTGATGGTC ACATGAATCT GCTGAAGGAA TTTGAGCTGT GGTTGCAGGA AGAGAATTCC
    AAACTAAACA AAATCTGTAG CACAAAAGCA TCTAGCAGCG AGGAAATGAA ATCCAGGCTC
    AGTAAACTGC AGAGATTACA GTTGCAAGTA CCCCGGGGTC AGCGCCTGTT TGAATCTTTG
    CTTGTTTCAA TGCCAGACAT GACAGTTACT GATAATTTAA GGCTGGAAGA CCTGCGCTAC
    CAATGGATGC TTTATAAATC CAAATTAAGA GATGCTGGAA GCTCATCGGG CATAAATACA
    TCTGAAGAGC TAAGAGGAAT AACAAAGAAG TCATCTGGGG GACTGTGCTC CTTCCTGCAC
    CGTGTGTGCT GTGCCGCACT TCCACTCCAG TTACTCCTTC TATTACTTCT CCTCTTGGCA
    TTGCTTATTC TTCTGATGCA GGAGAGCCAA AACTGCGCCC TTTCCAACAA CTTTGCCCGC
    TCCTTCAACC TGATGCTAAA ATACGAAAAA CCTCCTCCAA CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63894
    Clone ID Related Accession (Same CDS sequence) XM_031890678.1 , XM_004917083.3 , XM_004917084.3
    Accession Version XM_031890678.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAAC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GCAGTGCCTC TGACCGCAGT AACTCAGGTA GTGGTCAAAC ACTTCTAGGG
    AACAAGCAGG CATCCAGTGT TCTTCAAGTT GCTGATCCAC TTATCCCTTC AACAGAGGCT
    GGGGAAAGTT CAAGAACTTA TACTGGAAGT ATAGAAGAAA ATTATTTAGT GAGCACACCA
    GAAGAGTTTG GAGAGAGTTC AAGCTCTTTG ACGATAACTA CTGGATCCCT GGCAGACAAC
    CTGAAGAACC CAGTGAAACC AGATTCTCAG TACAATATGG TTTCTAGGAG AAGAAATAAG
    AAAGGTGTCA GCCTGGCAGA ATCTGTGGAT TTTGTGGATT CGTCTTATGT GCAAAGTAAA
    GAAGAGACAA AGACAGAGAA AGGTGATGGT CACATGAATC TGCTGAAGGA ATTTGAGCTG
    TGGTTGCAGG AAGAGAATTC CAAACTAAAC AAAATCTGTA GCACAAAAGC ATCTAGCAGC
    GAGGAAATGA AATCCAGGCT CAGTAAACTG CAGAGATTAC AGTTGCAAGT ACCCCGGGGT
    CAGCGCCTGT TTGAATCTTT GCTTGTTTCA ATGCCAGACA TGACAGTTAC TGATAATTTA
    AGGCTGGAAG ACCTGCGCTA CCAATGGATG CTTTATAAAT CCAAATTAAG AGATGCTGGA
    AGCTCATCGG GCATAAATAC ATCTGAAGAG CTAAGAGGAA TAACAAAGAA GTCATCTGGG
    GGACTGTGCT CCTTCCTGCA CCGTGTGTGC TGTGCCGCAC TTCCACTCCA GTTACTCCTT
    CTATTACTTC TCCTCTTGGC ATTGCTTATT CTTCTGATGC AGGAGAGCCA AAACTGCGCC
    CTTTCCAACA ACTTTGCCCG CTCCTTCAAC CTGATGCTAA AATACGAAAA ACCTCCTCCA
    ACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746538.1
    CDS436..4221
    Translation

    Target ORF information:

    RefSeq Version XM_031890678.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890678.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAAC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GCAGTGCCTC TGACCGCAGT AACTCAGGTA GTGGTCAAAC ACTTCTAGGG
    AACAAGCAGG CATCCAGTGT TCTTCAAGTT GCTGATCCAC TTATCCCTTC AACAGAGGCT
    GGGGAAAGTT CAAGAACTTA TACTGGAAGT ATAGAAGAAA ATTATTTAGT GAGCACACCA
    GAAGAGTTTG GAGAGAGTTC AAGCTCTTTG ACGATAACTA CTGGATCCCT GGCAGACAAC
    CTGAAGAACC CAGTGAAACC AGATTCTCAG TACAATATGG TTTCTAGGAG AAGAAATAAG
    AAAGGTGTCA GCCTGGCAGA ATCTGTGGAT TTTGTGGATT CGTCTTATGT GCAAAGTAAA
    GAAGAGACAA AGACAGAGAA AGGTGATGGT CACATGAATC TGCTGAAGGA ATTTGAGCTG
    TGGTTGCAGG AAGAGAATTC CAAACTAAAC AAAATCTGTA GCACAAAAGC ATCTAGCAGC
    GAGGAAATGA AATCCAGGCT CAGTAAACTG CAGAGATTAC AGTTGCAAGT ACCCCGGGGT
    CAGCGCCTGT TTGAATCTTT GCTTGTTTCA ATGCCAGACA TGACAGTTAC TGATAATTTA
    AGGCTGGAAG ACCTGCGCTA CCAATGGATG CTTTATAAAT CCAAATTAAG AGATGCTGGA
    AGCTCATCGG GCATAAATAC ATCTGAAGAG CTAAGAGGAA TAACAAAGAA GTCATCTGGG
    GGACTGTGCT CCTTCCTGCA CCGTGTGTGC TGTGCCGCAC TTCCACTCCA GTTACTCCTT
    CTATTACTTC TCCTCTTGGC ATTGCTTATT CTTCTGATGC AGGAGAGCCA AAACTGCGCC
    CTTTCCAACA ACTTTGCCCG CTCCTTCAAC CTGATGCTAA AATACGAAAA ACCTCCTCCA
    ACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63894
    Clone ID Related Accession (Same CDS sequence) XM_031890678.1 , XM_004917083.3 , XM_004917084.3
    Accession Version XM_004917083.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917083.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAAC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GCAGTGCCTC TGACCGCAGT AACTCAGGTA GTGGTCAAAC ACTTCTAGGG
    AACAAGCAGG CATCCAGTGT TCTTCAAGTT GCTGATCCAC TTATCCCTTC AACAGAGGCT
    GGGGAAAGTT CAAGAACTTA TACTGGAAGT ATAGAAGAAA ATTATTTAGT GAGCACACCA
    GAAGAGTTTG GAGAGAGTTC AAGCTCTTTG ACGATAACTA CTGGATCCCT GGCAGACAAC
    CTGAAGAACC CAGTGAAACC AGATTCTCAG TACAATATGG TTTCTAGGAG AAGAAATAAG
    AAAGGTGTCA GCCTGGCAGA ATCTGTGGAT TTTGTGGATT CGTCTTATGT GCAAAGTAAA
    GAAGAGACAA AGACAGAGAA AGGTGATGGT CACATGAATC TGCTGAAGGA ATTTGAGCTG
    TGGTTGCAGG AAGAGAATTC CAAACTAAAC AAAATCTGTA GCACAAAAGC ATCTAGCAGC
    GAGGAAATGA AATCCAGGCT CAGTAAACTG CAGAGATTAC AGTTGCAAGT ACCCCGGGGT
    CAGCGCCTGT TTGAATCTTT GCTTGTTTCA ATGCCAGACA TGACAGTTAC TGATAATTTA
    AGGCTGGAAG ACCTGCGCTA CCAATGGATG CTTTATAAAT CCAAATTAAG AGATGCTGGA
    AGCTCATCGG GCATAAATAC ATCTGAAGAG CTAAGAGGAA TAACAAAGAA GTCATCTGGG
    GGACTGTGCT CCTTCCTGCA CCGTGTGTGC TGTGCCGCAC TTCCACTCCA GTTACTCCTT
    CTATTACTTC TCCTCTTGGC ATTGCTTATT CTTCTGATGC AGGAGAGCCA AAACTGCGCC
    CTTTCCAACA ACTTTGCCCG CTCCTTCAAC CTGATGCTAA AATACGAAAA ACCTCCTCCA
    ACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917140.1
    CDS349..4134
    Translation

    Target ORF information:

    RefSeq Version XM_004917083.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917083.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAAC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GCAGTGCCTC TGACCGCAGT AACTCAGGTA GTGGTCAAAC ACTTCTAGGG
    AACAAGCAGG CATCCAGTGT TCTTCAAGTT GCTGATCCAC TTATCCCTTC AACAGAGGCT
    GGGGAAAGTT CAAGAACTTA TACTGGAAGT ATAGAAGAAA ATTATTTAGT GAGCACACCA
    GAAGAGTTTG GAGAGAGTTC AAGCTCTTTG ACGATAACTA CTGGATCCCT GGCAGACAAC
    CTGAAGAACC CAGTGAAACC AGATTCTCAG TACAATATGG TTTCTAGGAG AAGAAATAAG
    AAAGGTGTCA GCCTGGCAGA ATCTGTGGAT TTTGTGGATT CGTCTTATGT GCAAAGTAAA
    GAAGAGACAA AGACAGAGAA AGGTGATGGT CACATGAATC TGCTGAAGGA ATTTGAGCTG
    TGGTTGCAGG AAGAGAATTC CAAACTAAAC AAAATCTGTA GCACAAAAGC ATCTAGCAGC
    GAGGAAATGA AATCCAGGCT CAGTAAACTG CAGAGATTAC AGTTGCAAGT ACCCCGGGGT
    CAGCGCCTGT TTGAATCTTT GCTTGTTTCA ATGCCAGACA TGACAGTTAC TGATAATTTA
    AGGCTGGAAG ACCTGCGCTA CCAATGGATG CTTTATAAAT CCAAATTAAG AGATGCTGGA
    AGCTCATCGG GCATAAATAC ATCTGAAGAG CTAAGAGGAA TAACAAAGAA GTCATCTGGG
    GGACTGTGCT CCTTCCTGCA CCGTGTGTGC TGTGCCGCAC TTCCACTCCA GTTACTCCTT
    CTATTACTTC TCCTCTTGGC ATTGCTTATT CTTCTGATGC AGGAGAGCCA AAACTGCGCC
    CTTTCCAACA ACTTTGCCCG CTCCTTCAAC CTGATGCTAA AATACGAAAA ACCTCCTCCA
    ACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63894
    Clone ID Related Accession (Same CDS sequence) XM_031890678.1 , XM_004917083.3 , XM_004917084.3
    Accession Version XM_004917084.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917084.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAAC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GCAGTGCCTC TGACCGCAGT AACTCAGGTA GTGGTCAAAC ACTTCTAGGG
    AACAAGCAGG CATCCAGTGT TCTTCAAGTT GCTGATCCAC TTATCCCTTC AACAGAGGCT
    GGGGAAAGTT CAAGAACTTA TACTGGAAGT ATAGAAGAAA ATTATTTAGT GAGCACACCA
    GAAGAGTTTG GAGAGAGTTC AAGCTCTTTG ACGATAACTA CTGGATCCCT GGCAGACAAC
    CTGAAGAACC CAGTGAAACC AGATTCTCAG TACAATATGG TTTCTAGGAG AAGAAATAAG
    AAAGGTGTCA GCCTGGCAGA ATCTGTGGAT TTTGTGGATT CGTCTTATGT GCAAAGTAAA
    GAAGAGACAA AGACAGAGAA AGGTGATGGT CACATGAATC TGCTGAAGGA ATTTGAGCTG
    TGGTTGCAGG AAGAGAATTC CAAACTAAAC AAAATCTGTA GCACAAAAGC ATCTAGCAGC
    GAGGAAATGA AATCCAGGCT CAGTAAACTG CAGAGATTAC AGTTGCAAGT ACCCCGGGGT
    CAGCGCCTGT TTGAATCTTT GCTTGTTTCA ATGCCAGACA TGACAGTTAC TGATAATTTA
    AGGCTGGAAG ACCTGCGCTA CCAATGGATG CTTTATAAAT CCAAATTAAG AGATGCTGGA
    AGCTCATCGG GCATAAATAC ATCTGAAGAG CTAAGAGGAA TAACAAAGAA GTCATCTGGG
    GGACTGTGCT CCTTCCTGCA CCGTGTGTGC TGTGCCGCAC TTCCACTCCA GTTACTCCTT
    CTATTACTTC TCCTCTTGGC ATTGCTTATT CTTCTGATGC AGGAGAGCCA AAACTGCGCC
    CTTTCCAACA ACTTTGCCCG CTCCTTCAAC CTGATGCTAA AATACGAAAA ACCTCCTCCA
    ACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917141.1
    CDS470..4255
    Translation

    Target ORF information:

    RefSeq Version XM_004917084.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917084.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAAC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GCAGTGCCTC TGACCGCAGT AACTCAGGTA GTGGTCAAAC ACTTCTAGGG
    AACAAGCAGG CATCCAGTGT TCTTCAAGTT GCTGATCCAC TTATCCCTTC AACAGAGGCT
    GGGGAAAGTT CAAGAACTTA TACTGGAAGT ATAGAAGAAA ATTATTTAGT GAGCACACCA
    GAAGAGTTTG GAGAGAGTTC AAGCTCTTTG ACGATAACTA CTGGATCCCT GGCAGACAAC
    CTGAAGAACC CAGTGAAACC AGATTCTCAG TACAATATGG TTTCTAGGAG AAGAAATAAG
    AAAGGTGTCA GCCTGGCAGA ATCTGTGGAT TTTGTGGATT CGTCTTATGT GCAAAGTAAA
    GAAGAGACAA AGACAGAGAA AGGTGATGGT CACATGAATC TGCTGAAGGA ATTTGAGCTG
    TGGTTGCAGG AAGAGAATTC CAAACTAAAC AAAATCTGTA GCACAAAAGC ATCTAGCAGC
    GAGGAAATGA AATCCAGGCT CAGTAAACTG CAGAGATTAC AGTTGCAAGT ACCCCGGGGT
    CAGCGCCTGT TTGAATCTTT GCTTGTTTCA ATGCCAGACA TGACAGTTAC TGATAATTTA
    AGGCTGGAAG ACCTGCGCTA CCAATGGATG CTTTATAAAT CCAAATTAAG AGATGCTGGA
    AGCTCATCGG GCATAAATAC ATCTGAAGAG CTAAGAGGAA TAACAAAGAA GTCATCTGGG
    GGACTGTGCT CCTTCCTGCA CCGTGTGTGC TGTGCCGCAC TTCCACTCCA GTTACTCCTT
    CTATTACTTC TCCTCTTGGC ATTGCTTATT CTTCTGATGC AGGAGAGCCA AAACTGCGCC
    CTTTCCAACA ACTTTGCCCG CTCCTTCAAC CTGATGCTAA AATACGAAAA ACCTCCTCCA
    ACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63895
    Clone ID Related Accession (Same CDS sequence) XM_031890679.1
    Accession Version XM_031890679.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAAC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GTGCCTCTGA CCGCAGTAAC TCAGGTAGTG GTCAAACACT TCTAGGGAAC
    AAGCAGGCAT CCAGTGTTCT TCAAGTTGCT GATCCACTTA TCCCTTCAAC AGAGGCTGGG
    GAAAGTTCAA GAACTTATAC TGGAAGTATA GAAGAAAATT ATTTAGTGAG CACACCAGAA
    GAGTTTGGAG AGAGTTCAAG CTCTTTGACG ATAACTACTG GATCCCTGGC AGACAACCTG
    AAGAACCCAG TGAAACCAGA TTCTCAGTAC AATATGGTTT CTAGGAGAAG AAATAAGAAA
    GGTGTCAGCC TGGCAGAATC TGTGGATTTT GTGGATTCGT CTTATGTGCA AAGTAAAGAA
    GAGACAAAGA CAGAGAAAGG TGATGGTCAC ATGAATCTGC TGAAGGAATT TGAGCTGTGG
    TTGCAGGAAG AGAATTCCAA ACTAAACAAA ATCTGTAGCA CAAAAGCATC TAGCAGCGAG
    GAAATGAAAT CCAGGCTCAG TAAACTGCAG AGATTACAGT TGCAAGTACC CCGGGGTCAG
    CGCCTGTTTG AATCTTTGCT TGTTTCAATG CCAGACATGA CAGTTACTGA TAATTTAAGG
    CTGGAAGACC TGCGCTACCA ATGGATGCTT TATAAATCCA AATTAAGAGA TGCTGGAAGC
    TCATCGGGCA TAAATACATC TGAAGAGCTA AGAGGAATAA CAAAGAAGTC ATCTGGGGGA
    CTGTGCTCCT TCCTGCACCG TGTGTGCTGT GCCGCACTTC CACTCCAGTT ACTCCTTCTA
    TTACTTCTCC TCTTGGCATT GCTTATTCTT CTGATGCAGG AGAGCCAAAA CTGCGCCCTT
    TCCAACAACT TTGCCCGCTC CTTCAACCTG ATGCTAAAAT ACGAAAAACC TCCTCCAACT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746539.1
    CDS470..4252
    Translation

    Target ORF information:

    RefSeq Version XM_031890679.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890679.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAAC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GTGCCTCTGA CCGCAGTAAC TCAGGTAGTG GTCAAACACT TCTAGGGAAC
    AAGCAGGCAT CCAGTGTTCT TCAAGTTGCT GATCCACTTA TCCCTTCAAC AGAGGCTGGG
    GAAAGTTCAA GAACTTATAC TGGAAGTATA GAAGAAAATT ATTTAGTGAG CACACCAGAA
    GAGTTTGGAG AGAGTTCAAG CTCTTTGACG ATAACTACTG GATCCCTGGC AGACAACCTG
    AAGAACCCAG TGAAACCAGA TTCTCAGTAC AATATGGTTT CTAGGAGAAG AAATAAGAAA
    GGTGTCAGCC TGGCAGAATC TGTGGATTTT GTGGATTCGT CTTATGTGCA AAGTAAAGAA
    GAGACAAAGA CAGAGAAAGG TGATGGTCAC ATGAATCTGC TGAAGGAATT TGAGCTGTGG
    TTGCAGGAAG AGAATTCCAA ACTAAACAAA ATCTGTAGCA CAAAAGCATC TAGCAGCGAG
    GAAATGAAAT CCAGGCTCAG TAAACTGCAG AGATTACAGT TGCAAGTACC CCGGGGTCAG
    CGCCTGTTTG AATCTTTGCT TGTTTCAATG CCAGACATGA CAGTTACTGA TAATTTAAGG
    CTGGAAGACC TGCGCTACCA ATGGATGCTT TATAAATCCA AATTAAGAGA TGCTGGAAGC
    TCATCGGGCA TAAATACATC TGAAGAGCTA AGAGGAATAA CAAAGAAGTC ATCTGGGGGA
    CTGTGCTCCT TCCTGCACCG TGTGTGCTGT GCCGCACTTC CACTCCAGTT ACTCCTTCTA
    TTACTTCTCC TCTTGGCATT GCTTATTCTT CTGATGCAGG AGAGCCAAAA CTGCGCCCTT
    TCCAACAACT TTGCCCGCTC CTTCAACCTG ATGCTAAAAT ACGAAAAACC TCCTCCAACT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63896
    Clone ID Related Accession (Same CDS sequence) XM_031890680.1
    Accession Version XM_031890680.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAAC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGCAGT GCCTCTGACC GCAGTAACTC AGGTAGTGGT
    CAAACACTTC TAGGGAACAA GCAGGCATCC AGTGTTCTTC AAGTTGCTGA TCCACTTATC
    CCTTCAACAG AGGCTGGGGA AAGTTCAAGA ACTTATACTG GAAGTATAGA AGAAAATTAT
    TTAGTGAGCA CACCAGAAGA GTTTGGAGAG AGTTCAAGCT CTTTGACGAT AACTACTGGA
    TCCCTGGCAG ACAACCTGAA GAACCCAGTG AAACCAGATT CTCAGTACAA TATGGTTTCT
    AGGAGAAGAA ATAAGAAAGG TGTCAGCCTG GCAGAATCTG TGGATTTTGT GGATTCGTCT
    TATGTGCAAA GTAAAGAAGA GACAAAGACA GAGAAAGGTG ATGGTCACAT GAATCTGCTG
    AAGGAATTTG AGCTGTGGTT GCAGGAAGAG AATTCCAAAC TAAACAAAAT CTGTAGCACA
    AAAGCATCTA GCAGCGAGGA AATGAAATCC AGGCTCAGTA AACTGCAGAG ATTACAGTTG
    CAAGTACCCC GGGGTCAGCG CCTGTTTGAA TCTTTGCTTG TTTCAATGCC AGACATGACA
    GTTACTGATA ATTTAAGGCT GGAAGACCTG CGCTACCAAT GGATGCTTTA TAAATCCAAA
    TTAAGAGATG CTGGAAGCTC ATCGGGCATA AATACATCTG AAGAGCTAAG AGGAATAACA
    AAGAAGTCAT CTGGGGGACT GTGCTCCTTC CTGCACCGTG TGTGCTGTGC CGCACTTCCA
    CTCCAGTTAC TCCTTCTATT ACTTCTCCTC TTGGCATTGC TTATTCTTCT GATGCAGGAG
    AGCCAAAACT GCGCCCTTTC CAACAACTTT GCCCGCTCCT TCAACCTGAT GCTAAAATAC
    GAAAAACCTC CTCCAACTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746540.1
    CDS470..4210
    Translation

    Target ORF information:

    RefSeq Version XM_031890680.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890680.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAAC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGCAGT GCCTCTGACC GCAGTAACTC AGGTAGTGGT
    CAAACACTTC TAGGGAACAA GCAGGCATCC AGTGTTCTTC AAGTTGCTGA TCCACTTATC
    CCTTCAACAG AGGCTGGGGA AAGTTCAAGA ACTTATACTG GAAGTATAGA AGAAAATTAT
    TTAGTGAGCA CACCAGAAGA GTTTGGAGAG AGTTCAAGCT CTTTGACGAT AACTACTGGA
    TCCCTGGCAG ACAACCTGAA GAACCCAGTG AAACCAGATT CTCAGTACAA TATGGTTTCT
    AGGAGAAGAA ATAAGAAAGG TGTCAGCCTG GCAGAATCTG TGGATTTTGT GGATTCGTCT
    TATGTGCAAA GTAAAGAAGA GACAAAGACA GAGAAAGGTG ATGGTCACAT GAATCTGCTG
    AAGGAATTTG AGCTGTGGTT GCAGGAAGAG AATTCCAAAC TAAACAAAAT CTGTAGCACA
    AAAGCATCTA GCAGCGAGGA AATGAAATCC AGGCTCAGTA AACTGCAGAG ATTACAGTTG
    CAAGTACCCC GGGGTCAGCG CCTGTTTGAA TCTTTGCTTG TTTCAATGCC AGACATGACA
    GTTACTGATA ATTTAAGGCT GGAAGACCTG CGCTACCAAT GGATGCTTTA TAAATCCAAA
    TTAAGAGATG CTGGAAGCTC ATCGGGCATA AATACATCTG AAGAGCTAAG AGGAATAACA
    AAGAAGTCAT CTGGGGGACT GTGCTCCTTC CTGCACCGTG TGTGCTGTGC CGCACTTCCA
    CTCCAGTTAC TCCTTCTATT ACTTCTCCTC TTGGCATTGC TTATTCTTCT GATGCAGGAG
    AGCCAAAACT GCGCCCTTTC CAACAACTTT GCCCGCTCCT TCAACCTGAT GCTAAAATAC
    GAAAAACCTC CTCCAACTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63897
    Clone ID Related Accession (Same CDS sequence) XM_031890681.1
    Accession Version XM_031890681.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAAC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGTGCC TCTGACCGCA GTAACTCAGG TAGTGGTCAA
    ACACTTCTAG GGAACAAGCA GGCATCCAGT GTTCTTCAAG TTGCTGATCC ACTTATCCCT
    TCAACAGAGG CTGGGGAAAG TTCAAGAACT TATACTGGAA GTATAGAAGA AAATTATTTA
    GTGAGCACAC CAGAAGAGTT TGGAGAGAGT TCAAGCTCTT TGACGATAAC TACTGGATCC
    CTGGCAGACA ACCTGAAGAA CCCAGTGAAA CCAGATTCTC AGTACAATAT GGTTTCTAGG
    AGAAGAAATA AGAAAGGTGT CAGCCTGGCA GAATCTGTGG ATTTTGTGGA TTCGTCTTAT
    GTGCAAAGTA AAGAAGAGAC AAAGACAGAG AAAGGTGATG GTCACATGAA TCTGCTGAAG
    GAATTTGAGC TGTGGTTGCA GGAAGAGAAT TCCAAACTAA ACAAAATCTG TAGCACAAAA
    GCATCTAGCA GCGAGGAAAT GAAATCCAGG CTCAGTAAAC TGCAGAGATT ACAGTTGCAA
    GTACCCCGGG GTCAGCGCCT GTTTGAATCT TTGCTTGTTT CAATGCCAGA CATGACAGTT
    ACTGATAATT TAAGGCTGGA AGACCTGCGC TACCAATGGA TGCTTTATAA ATCCAAATTA
    AGAGATGCTG GAAGCTCATC GGGCATAAAT ACATCTGAAG AGCTAAGAGG AATAACAAAG
    AAGTCATCTG GGGGACTGTG CTCCTTCCTG CACCGTGTGT GCTGTGCCGC ACTTCCACTC
    CAGTTACTCC TTCTATTACT TCTCCTCTTG GCATTGCTTA TTCTTCTGAT GCAGGAGAGC
    CAAAACTGCG CCCTTTCCAA CAACTTTGCC CGCTCCTTCA ACCTGATGCT AAAATACGAA
    AAACCTCCTC CAACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746541.1
    CDS470..4207
    Translation

    Target ORF information:

    RefSeq Version XM_031890681.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890681.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAAC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGTGCC TCTGACCGCA GTAACTCAGG TAGTGGTCAA
    ACACTTCTAG GGAACAAGCA GGCATCCAGT GTTCTTCAAG TTGCTGATCC ACTTATCCCT
    TCAACAGAGG CTGGGGAAAG TTCAAGAACT TATACTGGAA GTATAGAAGA AAATTATTTA
    GTGAGCACAC CAGAAGAGTT TGGAGAGAGT TCAAGCTCTT TGACGATAAC TACTGGATCC
    CTGGCAGACA ACCTGAAGAA CCCAGTGAAA CCAGATTCTC AGTACAATAT GGTTTCTAGG
    AGAAGAAATA AGAAAGGTGT CAGCCTGGCA GAATCTGTGG ATTTTGTGGA TTCGTCTTAT
    GTGCAAAGTA AAGAAGAGAC AAAGACAGAG AAAGGTGATG GTCACATGAA TCTGCTGAAG
    GAATTTGAGC TGTGGTTGCA GGAAGAGAAT TCCAAACTAA ACAAAATCTG TAGCACAAAA
    GCATCTAGCA GCGAGGAAAT GAAATCCAGG CTCAGTAAAC TGCAGAGATT ACAGTTGCAA
    GTACCCCGGG GTCAGCGCCT GTTTGAATCT TTGCTTGTTT CAATGCCAGA CATGACAGTT
    ACTGATAATT TAAGGCTGGA AGACCTGCGC TACCAATGGA TGCTTTATAA ATCCAAATTA
    AGAGATGCTG GAAGCTCATC GGGCATAAAT ACATCTGAAG AGCTAAGAGG AATAACAAAG
    AAGTCATCTG GGGGACTGTG CTCCTTCCTG CACCGTGTGT GCTGTGCCGC ACTTCCACTC
    CAGTTACTCC TTCTATTACT TCTCCTCTTG GCATTGCTTA TTCTTCTGAT GCAGGAGAGC
    CAAAACTGCG CCCTTTCCAA CAACTTTGCC CGCTCCTTCA ACCTGATGCT AAAATACGAA
    AAACCTCCTC CAACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa63898
    Clone ID Related Accession (Same CDS sequence) XM_018096767.2
    Accession Version XM_018096767.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096767.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATAACAC TGAAGGGCCC AGATCTGCCC TGGAGGCCAG GCTCAGGGAG ACAGAGTCGC 
    ATTGAGTGGG TTTGGCTGCA CTGGAACGAG TACCTGAAAG CCAGAGATGA GTTTTATCTG
    TGGGTCCATA ACATGAAACT GACCCTGGAA CCAGATGTGG AACTTCAGCT GGGCGTTAAA
    GAGAAGCGCT GGCAATATGA ACAAGCACAA GTGCTGCTAA GAGATGTCAA CAACCAGTCA
    TGCCTGTTGG ACAGACTACT GGAGGAAGCC GCCTCTCTAT ACAACAGGAT TGGAGACCCC
    AGTGTGGATG AAACAGTGCA GAGAGAAATG ATGGCTGAAT ACAAACAAAT AAAGAAGAAA
    GCCAAGGAAA GGGCAAGTCA TCTTGAAAAT ATGTTGAAAG AACATGAAGC TTATGATAGG
    GATGTCAATG AATTCCGGTC TTGGCTGACC AGTGTGATTG AAAAGCTGAA GTGTTGTGTT
    GGAGAGGCCA TGGAATCAAC AGAATACAGG CTCAGTATGC TGCAGGAAAT CAGCAAAGAG
    GTACACAGTG GTGGTAAAAA GTTGGAAGAC CTTGAGGGCA AGTCACTTGA AGTGATAAAG
    AACACATCCC CTCTAGGTGC GGAGAATATC TGCAAGGAGC TGGAGGAGTT GCACAAAGCT
    CTAGCAGATC TGAAACGCAT GAATGATGAT GAAGAACAGA GTCTTCTCAA GGCTCATAAT
    TCTGAAAATG CCTTCCTACT TTTGGCTAGG CAACTAGAAG CCAACATCAA TGAGTTCCGA
    AAAGCCATCC AGAGACTGGA GGAAAGTCTG GAATCAGGAG AAAGGGTGAA GAGTGAAGAT
    GAACTGGTAG CTCTGTGGAA AACATTAAAC GCCACCAAGT CTGCTTTAGC AGCAGAGGAA
    ACAAAAGGTG AAGGGGTAAA GGTTCAGCTG AAGGAACTCT TCAAGTTCTC CAAGGATGTC
    CAGCCGCTTT CTGATGAAGT CATTGCTGCA ATTCGAGAAT ACCAAAGGGC TAAAAGCAAA
    GCCTTCAAGC TGAGTACTGA GACTGAATCG GCACTCATAC AGCACTTCCA GATTCCGCTT
    CTGGAATTTC ACCATTGGAA ACGCATAGGT GACAGAGTCC TGGATACCAC ATCAACTCCT
    GTGAGCGATT ATGCTCTGAA CCATGACTGC TTGCTACAGA TTGAGAGGTT GCTGGAGGAG
    AGTGCTAGTA TTGAAGAAAA ACTGGGTATT CTGCAGACAA AGAAGGAACG AATCAGTGGG
    GTGCTTGGTG AACAGAAGGC AGAAACCTTT TTTACTGAGG TGGCATTTGC TTCAAGGGAA
    AGGGATTCTC TCCATGCTGT TCTATTGGAG AAAAAGAAAA TTTTACTGAG TTTGGCTTCC
    CAGAAGAAAG AATTTGATGC TGCTTTTGAA CAGTTGCGGA AGAAGGTGTT GACTTTAAGA
    ATTAAAGCTG GCAAAGAAAG TGAATTACAG CCAGACCTTG TAGGGAAAGA AACACAACTG
    CAGAGATTTC AGATGTTTAA GGATGATTTG TTAAAACTGG AACCTGAATT TAAAGAGGTA
    GGGTCAATGG CTGAGTCAAG TCCCACCCTC AAGCTTAAAG TCAGCCAGCT GTCTTCAGAA
    TACCTTACTT TACACAAGTC ACTTGAGATT AAAATACGAA AAAGCAAAGC ATACATTAAT
    GACCATCGGA TATTTAATGA GAAGCTGCTA GAGCTTCAGC GCTGGCTTAT GGTGACAAAG
    CAGGAGTTGG AATCATTTCA GGATAGTGAT GGGCATTGGG ATTTTGAATC CCGAGAGAAG
    GAAATACAGA TATTGTTGTC TGAGGTACCA GAAAAAGATA TCCAGCTACA ACAGGTAGAA
    TCTCAAGGTT TGCTAGTGAT GGAGACCTCC TCTCCAGAAG GAGCTGCCCA CATTCAGACT
    GAGCTGAGGG AGCTTAATGC CTCCTGGCTA AATCTGAAAC TACTCTGGGG GGCACTGGCA
    AGCGCGTTTA AAGACAGAGA GTTCCAGAGA CTGGCACTAC TGCCACCTAA AAGTACATCT
    GAGGACCACC TACATCAGAC AACTGACCTC CTCCTGAATA TGGAACAATC CTGGACTTCA
    TCTCAGTCTG ATAATAGCAA CAATGGCATG AACAACAGTA ATGATTCTGA AGAATTTGGA
    CCAAGTGGAA TTGTGGGCAG AATTGTCTAT GACACCAGTC CCCAAAGCTT AACATTAAGA
    TCAGAGAAAA GTCGGATACC ACAAAGGATA AACAAAACAA CCAGTTTGCT CCAAGAAAAG
    GATCCATCCT CTACAACAGC CTCTGATAAG AGTTTTGTCT CTCTGGAAGT AAATACTAGC
    ATGGCCGGTG ACTCCAGTCC ATCCAGGATA ACTGTGTGGT CAGACAAAAG CCGAATACCA
    ATGAGGATAA ACCAAACCTC CAGTTCCAGT GTTCTGCCAG AAAACTGTTC ACCCTTCACA
    CCACAAGAGT CTGGGATGAG TCCAAGAACT GTGAAGAGTG GACTTTTGAA TGACACCACG
    CCTCCCGATG TGGCAGTCAG ATCAGACACT AGTCAGCCGC AAAAAAGGAG AACGAAAGCA
    TCTAGGTTAG AGAGCCCACA TCCAACTATA TATTTTGATG CACATTTCTG CAGTGCCTCT
    GACCGCAGTA ACTCAGGTAG TGGTCAAACA CTTCTAGGGA ACAAGCAGGC ATCCAGTGTT
    CTTCAAGTTG CTGATCCACT TATCCCTTCA ACAGAGGCTG GGGAAAGTTC AAGAACTTAT
    ACTGGAAGTA TAGAAGAAAA TTATTTAGTG AGCACACCAG AAGAGTTTGG AGAGAGTTCA
    AGCTCTTTGA CGATAACTAC TGGATCCCTG GCAGACAACC TGAAGAACCC AGTGAAACCA
    GATTCTCAGT ACAATATGGT TTCTAGGAGA AGAAATAAGA AAGGTGTCAG CCTGGCAGAA
    TCTGTGGATT TTGTGGATTC GTCTTATGTG CAAAGTAAAG AAGAGACAAA GACAGAGAAA
    GGTGATGGTC ACATGAATCT GCTGAAGGAA TTTGAGCTGT GGTTGCAGGA AGAGAATTCC
    AAACTAAACA AAATCTGTAG CACAAAAGCA TCTAGCAGCG AGGAAATGAA ATCCAGGCTC
    AGTAAACTGC AGAGATTACA GTTGCAAGTA CCCCGGGGTC AGCGCCTGTT TGAATCTTTG
    CTTGTTTCAA TGCCAGACAT GACAGTTACT GATAATTTAA GGCTGGAAGA CCTGCGCTAC
    CAATGGATGC TTTATAAATC CAAATTAAGA GATGCTGGAA GCTCATCGGG CATAAATACA
    TCTGAAGAGC TAAGAGGAAT AACAAAGAAG TCATCTGGGG GACTGTGCTC CTTCCTGCAC
    CGTGTGTGCT GTGCCGCACT TCCACTCCAG TTACTCCTTC TATTACTTCT CCTCTTGGCA
    TTGCTTATTC TTCTGATGCA GGAGAGCCAA AACTGCGCCC TTTCCAACAA CTTTGCCCGC
    TCCTTCAACC TGATGCTAAA ATACGAAAAA CCTCCTCCAA CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952256.1
    CDS99..3623
    Translation

    Target ORF information:

    RefSeq Version XM_018096767.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096767.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATAACAC TGAAGGGCCC AGATCTGCCC TGGAGGCCAG GCTCAGGGAG ACAGAGTCGC 
    ATTGAGTGGG TTTGGCTGCA CTGGAACGAG TACCTGAAAG CCAGAGATGA GTTTTATCTG
    TGGGTCCATA ACATGAAACT GACCCTGGAA CCAGATGTGG AACTTCAGCT GGGCGTTAAA
    GAGAAGCGCT GGCAATATGA ACAAGCACAA GTGCTGCTAA GAGATGTCAA CAACCAGTCA
    TGCCTGTTGG ACAGACTACT GGAGGAAGCC GCCTCTCTAT ACAACAGGAT TGGAGACCCC
    AGTGTGGATG AAACAGTGCA GAGAGAAATG ATGGCTGAAT ACAAACAAAT AAAGAAGAAA
    GCCAAGGAAA GGGCAAGTCA TCTTGAAAAT ATGTTGAAAG AACATGAAGC TTATGATAGG
    GATGTCAATG AATTCCGGTC TTGGCTGACC AGTGTGATTG AAAAGCTGAA GTGTTGTGTT
    GGAGAGGCCA TGGAATCAAC AGAATACAGG CTCAGTATGC TGCAGGAAAT CAGCAAAGAG
    GTACACAGTG GTGGTAAAAA GTTGGAAGAC CTTGAGGGCA AGTCACTTGA AGTGATAAAG
    AACACATCCC CTCTAGGTGC GGAGAATATC TGCAAGGAGC TGGAGGAGTT GCACAAAGCT
    CTAGCAGATC TGAAACGCAT GAATGATGAT GAAGAACAGA GTCTTCTCAA GGCTCATAAT
    TCTGAAAATG CCTTCCTACT TTTGGCTAGG CAACTAGAAG CCAACATCAA TGAGTTCCGA
    AAAGCCATCC AGAGACTGGA GGAAAGTCTG GAATCAGGAG AAAGGGTGAA GAGTGAAGAT
    GAACTGGTAG CTCTGTGGAA AACATTAAAC GCCACCAAGT CTGCTTTAGC AGCAGAGGAA
    ACAAAAGGTG AAGGGGTAAA GGTTCAGCTG AAGGAACTCT TCAAGTTCTC CAAGGATGTC
    CAGCCGCTTT CTGATGAAGT CATTGCTGCA ATTCGAGAAT ACCAAAGGGC TAAAAGCAAA
    GCCTTCAAGC TGAGTACTGA GACTGAATCG GCACTCATAC AGCACTTCCA GATTCCGCTT
    CTGGAATTTC ACCATTGGAA ACGCATAGGT GACAGAGTCC TGGATACCAC ATCAACTCCT
    GTGAGCGATT ATGCTCTGAA CCATGACTGC TTGCTACAGA TTGAGAGGTT GCTGGAGGAG
    AGTGCTAGTA TTGAAGAAAA ACTGGGTATT CTGCAGACAA AGAAGGAACG AATCAGTGGG
    GTGCTTGGTG AACAGAAGGC AGAAACCTTT TTTACTGAGG TGGCATTTGC TTCAAGGGAA
    AGGGATTCTC TCCATGCTGT TCTATTGGAG AAAAAGAAAA TTTTACTGAG TTTGGCTTCC
    CAGAAGAAAG AATTTGATGC TGCTTTTGAA CAGTTGCGGA AGAAGGTGTT GACTTTAAGA
    ATTAAAGCTG GCAAAGAAAG TGAATTACAG CCAGACCTTG TAGGGAAAGA AACACAACTG
    CAGAGATTTC AGATGTTTAA GGATGATTTG TTAAAACTGG AACCTGAATT TAAAGAGGTA
    GGGTCAATGG CTGAGTCAAG TCCCACCCTC AAGCTTAAAG TCAGCCAGCT GTCTTCAGAA
    TACCTTACTT TACACAAGTC ACTTGAGATT AAAATACGAA AAAGCAAAGC ATACATTAAT
    GACCATCGGA TATTTAATGA GAAGCTGCTA GAGCTTCAGC GCTGGCTTAT GGTGACAAAG
    CAGGAGTTGG AATCATTTCA GGATAGTGAT GGGCATTGGG ATTTTGAATC CCGAGAGAAG
    GAAATACAGA TATTGTTGTC TGAGGTACCA GAAAAAGATA TCCAGCTACA ACAGGTAGAA
    TCTCAAGGTT TGCTAGTGAT GGAGACCTCC TCTCCAGAAG GAGCTGCCCA CATTCAGACT
    GAGCTGAGGG AGCTTAATGC CTCCTGGCTA AATCTGAAAC TACTCTGGGG GGCACTGGCA
    AGCGCGTTTA AAGACAGAGA GTTCCAGAGA CTGGCACTAC TGCCACCTAA AAGTACATCT
    GAGGACCACC TACATCAGAC AACTGACCTC CTCCTGAATA TGGAACAATC CTGGACTTCA
    TCTCAGTCTG ATAATAGCAA CAATGGCATG AACAACAGTA ATGATTCTGA AGAATTTGGA
    CCAAGTGGAA TTGTGGGCAG AATTGTCTAT GACACCAGTC CCCAAAGCTT AACATTAAGA
    TCAGAGAAAA GTCGGATACC ACAAAGGATA AACAAAACAA CCAGTTTGCT CCAAGAAAAG
    GATCCATCCT CTACAACAGC CTCTGATAAG AGTTTTGTCT CTCTGGAAGT AAATACTAGC
    ATGGCCGGTG ACTCCAGTCC ATCCAGGATA ACTGTGTGGT CAGACAAAAG CCGAATACCA
    ATGAGGATAA ACCAAACCTC CAGTTCCAGT GTTCTGCCAG AAAACTGTTC ACCCTTCACA
    CCACAAGAGT CTGGGATGAG TCCAAGAACT GTGAAGAGTG GACTTTTGAA TGACACCACG
    CCTCCCGATG TGGCAGTCAG ATCAGACACT AGTCAGCCGC AAAAAAGGAG AACGAAAGCA
    TCTAGGTTAG AGAGCCCACA TCCAACTATA TATTTTGATG CACATTTCTG CAGTGCCTCT
    GACCGCAGTA ACTCAGGTAG TGGTCAAACA CTTCTAGGGA ACAAGCAGGC ATCCAGTGTT
    CTTCAAGTTG CTGATCCACT TATCCCTTCA ACAGAGGCTG GGGAAAGTTC AAGAACTTAT
    ACTGGAAGTA TAGAAGAAAA TTATTTAGTG AGCACACCAG AAGAGTTTGG AGAGAGTTCA
    AGCTCTTTGA CGATAACTAC TGGATCCCTG GCAGACAACC TGAAGAACCC AGTGAAACCA
    GATTCTCAGT ACAATATGGT TTCTAGGAGA AGAAATAAGA AAGGTGTCAG CCTGGCAGAA
    TCTGTGGATT TTGTGGATTC GTCTTATGTG CAAAGTAAAG AAGAGACAAA GACAGAGAAA
    GGTGATGGTC ACATGAATCT GCTGAAGGAA TTTGAGCTGT GGTTGCAGGA AGAGAATTCC
    AAACTAAACA AAATCTGTAG CACAAAAGCA TCTAGCAGCG AGGAAATGAA ATCCAGGCTC
    AGTAAACTGC AGAGATTACA GTTGCAAGTA CCCCGGGGTC AGCGCCTGTT TGAATCTTTG
    CTTGTTTCAA TGCCAGACAT GACAGTTACT GATAATTTAA GGCTGGAAGA CCTGCGCTAC
    CAATGGATGC TTTATAAATC CAAATTAAGA GATGCTGGAA GCTCATCGGG CATAAATACA
    TCTGAAGAGC TAAGAGGAAT AACAAAGAAG TCATCTGGGG GACTGTGCTC CTTCCTGCAC
    CGTGTGTGCT GTGCCGCACT TCCACTCCAG TTACTCCTTC TATTACTTCT CCTCTTGGCA
    TTGCTTATTC TTCTGATGCA GGAGAGCCAA AACTGCGCCC TTTCCAACAA CTTTGCCCGC
    TCCTTCAACC TGATGCTAAA ATACGAAAAA CCTCCTCCAA CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14725
    Clone ID Related Accession (Same CDS sequence) XM_004917083.2 , XM_004917084.2
    Accession Version XM_004917083.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAGC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GCAGTGCCTC TGACCGCAGT AACTCAGGTA GTGGTCAAAC ACTTCTAGGG
    AACAAGCAGG CATCCAGTGT TCTTCAAGTT GCTGATCCAC TTATCCCTTC AACAGAGGCT
    GGGGAAAGTT CAAGAACTTA TACTGGAAGT ATAGAAGAAA ATTATTTAGT GAGCACACCA
    GAAGAGTTTG GAGAGAGTTC AAGCTCTTTG ACGATAACTA CTGGATCCCT GGCAGACAAC
    CTGAAGAACC CAGTGAAACC AGATTCTCAG TACAATATGG TTTCTAGGAG AAGAAATAAG
    AAAGGTGTCA GCCTGGCAGA ATCTGTGGAT TTTGTGGATT CGTCTTATGT GCAAAGTAAA
    GAAGAGACAA AGACAGAGAA AGGTGATGGT CACATGAATC TGCTGAAGGA ATTTGAGCTG
    TGGTTGCAGG AAGAGAATTC CAAACTAAAC AAAATCTGTA GCACAAAAGC ATCTAGCAGC
    GAGGAAATGA AATCCAGGCT CAGTAAACTG CAGAGATTAC AGTTGCAAGT ACCCCGGGGT
    CAGCGCCTGT TTGAATCTTT GCTTGTTTCA ATGCCAGACA TGACAGTTAC TGATAATTTA
    AGGCTGGAAG ACCTGCGCTA CCAATGGATG CTTTATAAAT CCAAATTAAG AGATGCTGGA
    AGCTCATCGG GCATAAATAC ATCTGAAGAG CTAAGAGGAA TAACAAAGAA GTCATCTGGG
    GGACTGTGCT CCTTCCTGCA CCGTGTGTGC TGTGCCGCAC TTCCACTCCA GTTACTCCTT
    CTATTACTTC TCCTCTTGGC ATTGCTTATT CTTCTGATGC AGGAGAGCCA AAACTGCGCC
    CTTTCCAACA ACTTTGCCCG CTCCTTCAAC CTGATGCTAA AATACGAAAA ACCTCCTCCA
    ACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917140.1
    CDS363..4148
    Translation

    Target ORF information:

    RefSeq Version XM_004917083.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917083.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAGC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GCAGTGCCTC TGACCGCAGT AACTCAGGTA GTGGTCAAAC ACTTCTAGGG
    AACAAGCAGG CATCCAGTGT TCTTCAAGTT GCTGATCCAC TTATCCCTTC AACAGAGGCT
    GGGGAAAGTT CAAGAACTTA TACTGGAAGT ATAGAAGAAA ATTATTTAGT GAGCACACCA
    GAAGAGTTTG GAGAGAGTTC AAGCTCTTTG ACGATAACTA CTGGATCCCT GGCAGACAAC
    CTGAAGAACC CAGTGAAACC AGATTCTCAG TACAATATGG TTTCTAGGAG AAGAAATAAG
    AAAGGTGTCA GCCTGGCAGA ATCTGTGGAT TTTGTGGATT CGTCTTATGT GCAAAGTAAA
    GAAGAGACAA AGACAGAGAA AGGTGATGGT CACATGAATC TGCTGAAGGA ATTTGAGCTG
    TGGTTGCAGG AAGAGAATTC CAAACTAAAC AAAATCTGTA GCACAAAAGC ATCTAGCAGC
    GAGGAAATGA AATCCAGGCT CAGTAAACTG CAGAGATTAC AGTTGCAAGT ACCCCGGGGT
    CAGCGCCTGT TTGAATCTTT GCTTGTTTCA ATGCCAGACA TGACAGTTAC TGATAATTTA
    AGGCTGGAAG ACCTGCGCTA CCAATGGATG CTTTATAAAT CCAAATTAAG AGATGCTGGA
    AGCTCATCGG GCATAAATAC ATCTGAAGAG CTAAGAGGAA TAACAAAGAA GTCATCTGGG
    GGACTGTGCT CCTTCCTGCA CCGTGTGTGC TGTGCCGCAC TTCCACTCCA GTTACTCCTT
    CTATTACTTC TCCTCTTGGC ATTGCTTATT CTTCTGATGC AGGAGAGCCA AAACTGCGCC
    CTTTCCAACA ACTTTGCCCG CTCCTTCAAC CTGATGCTAA AATACGAAAA ACCTCCTCCA
    ACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14725
    Clone ID Related Accession (Same CDS sequence) XM_004917083.2 , XM_004917084.2
    Accession Version XM_004917084.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAGC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GCAGTGCCTC TGACCGCAGT AACTCAGGTA GTGGTCAAAC ACTTCTAGGG
    AACAAGCAGG CATCCAGTGT TCTTCAAGTT GCTGATCCAC TTATCCCTTC AACAGAGGCT
    GGGGAAAGTT CAAGAACTTA TACTGGAAGT ATAGAAGAAA ATTATTTAGT GAGCACACCA
    GAAGAGTTTG GAGAGAGTTC AAGCTCTTTG ACGATAACTA CTGGATCCCT GGCAGACAAC
    CTGAAGAACC CAGTGAAACC AGATTCTCAG TACAATATGG TTTCTAGGAG AAGAAATAAG
    AAAGGTGTCA GCCTGGCAGA ATCTGTGGAT TTTGTGGATT CGTCTTATGT GCAAAGTAAA
    GAAGAGACAA AGACAGAGAA AGGTGATGGT CACATGAATC TGCTGAAGGA ATTTGAGCTG
    TGGTTGCAGG AAGAGAATTC CAAACTAAAC AAAATCTGTA GCACAAAAGC ATCTAGCAGC
    GAGGAAATGA AATCCAGGCT CAGTAAACTG CAGAGATTAC AGTTGCAAGT ACCCCGGGGT
    CAGCGCCTGT TTGAATCTTT GCTTGTTTCA ATGCCAGACA TGACAGTTAC TGATAATTTA
    AGGCTGGAAG ACCTGCGCTA CCAATGGATG CTTTATAAAT CCAAATTAAG AGATGCTGGA
    AGCTCATCGG GCATAAATAC ATCTGAAGAG CTAAGAGGAA TAACAAAGAA GTCATCTGGG
    GGACTGTGCT CCTTCCTGCA CCGTGTGTGC TGTGCCGCAC TTCCACTCCA GTTACTCCTT
    CTATTACTTC TCCTCTTGGC ATTGCTTATT CTTCTGATGC AGGAGAGCCA AAACTGCGCC
    CTTTCCAACA ACTTTGCCCG CTCCTTCAAC CTGATGCTAA AATACGAAAA ACCTCCTCCA
    ACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917141.1
    CDS484..4269
    Translation

    Target ORF information:

    RefSeq Version XM_004917084.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917084.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAGC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGGTTA GAGAGCCCAC ATCCAACTAT ATATTTTGAT
    GCACATTTCT GCAGTGCCTC TGACCGCAGT AACTCAGGTA GTGGTCAAAC ACTTCTAGGG
    AACAAGCAGG CATCCAGTGT TCTTCAAGTT GCTGATCCAC TTATCCCTTC AACAGAGGCT
    GGGGAAAGTT CAAGAACTTA TACTGGAAGT ATAGAAGAAA ATTATTTAGT GAGCACACCA
    GAAGAGTTTG GAGAGAGTTC AAGCTCTTTG ACGATAACTA CTGGATCCCT GGCAGACAAC
    CTGAAGAACC CAGTGAAACC AGATTCTCAG TACAATATGG TTTCTAGGAG AAGAAATAAG
    AAAGGTGTCA GCCTGGCAGA ATCTGTGGAT TTTGTGGATT CGTCTTATGT GCAAAGTAAA
    GAAGAGACAA AGACAGAGAA AGGTGATGGT CACATGAATC TGCTGAAGGA ATTTGAGCTG
    TGGTTGCAGG AAGAGAATTC CAAACTAAAC AAAATCTGTA GCACAAAAGC ATCTAGCAGC
    GAGGAAATGA AATCCAGGCT CAGTAAACTG CAGAGATTAC AGTTGCAAGT ACCCCGGGGT
    CAGCGCCTGT TTGAATCTTT GCTTGTTTCA ATGCCAGACA TGACAGTTAC TGATAATTTA
    AGGCTGGAAG ACCTGCGCTA CCAATGGATG CTTTATAAAT CCAAATTAAG AGATGCTGGA
    AGCTCATCGG GCATAAATAC ATCTGAAGAG CTAAGAGGAA TAACAAAGAA GTCATCTGGG
    GGACTGTGCT CCTTCCTGCA CCGTGTGTGC TGTGCCGCAC TTCCACTCCA GTTACTCCTT
    CTATTACTTC TCCTCTTGGC ATTGCTTATT CTTCTGATGC AGGAGAGCCA AAACTGCGCC
    CTTTCCAACA ACTTTGCCCG CTCCTTCAAC CTGATGCTAA AATACGAAAA ACCTCCTCCA
    ACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14727
    Clone ID Related Accession (Same CDS sequence) XM_002936067.4
    Accession Version XM_002936067.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936067.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAGC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGCAGT GCCTCTGACC GCAGTAACTC AGGTAGTGGT
    CAAACACTTC TAGGGAACAA GCAGGCATCC AGTGTTCTTC AAGTTGCTGA TCCACTTATC
    CCTTCAACAG AGGCTGGGGA AAGTTCAAGA ACTTATACTG GAAGTATAGA AGAAAATTAT
    TTAGTGAGCA CACCAGAAGA GTTTGGAGAG AGTTCAAGCT CTTTGACGAT AACTACTGGA
    TCCCTGGCAG ACAACCTGAA GAACCCAGTG AAACCAGATT CTCAGTACAA TATGGTTTCT
    AGGAGAAGAA ATAAGAAAGG TGTCAGCCTG GCAGAATCTG TGGATTTTGT GGATTCGTCT
    TATGTGCAAA GTAAAGAAGA GACAAAGACA GAGAAAGGTG ATGGTCACAT GAATCTGCTG
    AAGGAATTTG AGCTGTGGTT GCAGGAAGAG AATTCCAAAC TAAACAAAAT CTGTAGCACA
    AAAGCATCTA GCAGCGAGGA AATGAAATCC AGGCTCAGTA AACTGCAGAG ATTACAGTTG
    CAAGTACCCC GGGGTCAGCG CCTGTTTGAA TCTTTGCTTG TTTCAATGCC AGACATGACA
    GTTACTGATA ATTTAAGGCT GGAAGACCTG CGCTACCAAT GGATGCTTTA TAAATCCAAA
    TTAAGAGATG CTGGAAGCTC ATCGGGCATA AATACATCTG AAGAGCTAAG AGGAATAACA
    AAGAAGTCAT CTGGGGGACT GTGCTCCTTC CTGCACCGTG TGTGCTGTGC CGCACTTCCA
    CTCCAGTTAC TCCTTCTATT ACTTCTCCTC TTGGCATTGC TTATTCTTCT GATGCAGGAG
    AGCCAAAACT GCGCCCTTTC CAACAACTTT GCCCGCTCCT TCAACCTGAT GCTAAAATAC
    GAAAAACCTC CTCCAACTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002936113.2
    CDS362..4102
    Translation

    Target ORF information:

    RefSeq Version XM_002936067.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002936067.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAGC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGCAGT GCCTCTGACC GCAGTAACTC AGGTAGTGGT
    CAAACACTTC TAGGGAACAA GCAGGCATCC AGTGTTCTTC AAGTTGCTGA TCCACTTATC
    CCTTCAACAG AGGCTGGGGA AAGTTCAAGA ACTTATACTG GAAGTATAGA AGAAAATTAT
    TTAGTGAGCA CACCAGAAGA GTTTGGAGAG AGTTCAAGCT CTTTGACGAT AACTACTGGA
    TCCCTGGCAG ACAACCTGAA GAACCCAGTG AAACCAGATT CTCAGTACAA TATGGTTTCT
    AGGAGAAGAA ATAAGAAAGG TGTCAGCCTG GCAGAATCTG TGGATTTTGT GGATTCGTCT
    TATGTGCAAA GTAAAGAAGA GACAAAGACA GAGAAAGGTG ATGGTCACAT GAATCTGCTG
    AAGGAATTTG AGCTGTGGTT GCAGGAAGAG AATTCCAAAC TAAACAAAAT CTGTAGCACA
    AAAGCATCTA GCAGCGAGGA AATGAAATCC AGGCTCAGTA AACTGCAGAG ATTACAGTTG
    CAAGTACCCC GGGGTCAGCG CCTGTTTGAA TCTTTGCTTG TTTCAATGCC AGACATGACA
    GTTACTGATA ATTTAAGGCT GGAAGACCTG CGCTACCAAT GGATGCTTTA TAAATCCAAA
    TTAAGAGATG CTGGAAGCTC ATCGGGCATA AATACATCTG AAGAGCTAAG AGGAATAACA
    AAGAAGTCAT CTGGGGGACT GTGCTCCTTC CTGCACCGTG TGTGCTGTGC CGCACTTCCA
    CTCCAGTTAC TCCTTCTATT ACTTCTCCTC TTGGCATTGC TTATTCTTCT GATGCAGGAG
    AGCCAAAACT GCGCCCTTTC CAACAACTTT GCCCGCTCCT TCAACCTGAT GCTAAAATAC
    GAAAAACCTC CTCCAACTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14728
    Clone ID Related Accession (Same CDS sequence) XM_004917086.3
    Accession Version XM_004917086.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917086.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAGC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGTGCC TCTGACCGCA GTAACTCAGG TAGTGGTCAA
    ACACTTCTAG GGAACAAGCA GGCATCCAGT GTTCTTCAAG TTGCTGATCC ACTTATCCCT
    TCAACAGAGG CTGGGGAAAG TTCAAGAACT TATACTGGAA GTATAGAAGA AAATTATTTA
    GTGAGCACAC CAGAAGAGTT TGGAGAGAGT TCAAGCTCTT TGACGATAAC TACTGGATCC
    CTGGCAGACA ACCTGAAGAA CCCAGTGAAA CCAGATTCTC AGTACAATAT GGTTTCTAGG
    AGAAGAAATA AGAAAGGTGT CAGCCTGGCA GAATCTGTGG ATTTTGTGGA TTCGTCTTAT
    GTGCAAAGTA AAGAAGAGAC AAAGACAGAG AAAGGTGATG GTCACATGAA TCTGCTGAAG
    GAATTTGAGC TGTGGTTGCA GGAAGAGAAT TCCAAACTAA ACAAAATCTG TAGCACAAAA
    GCATCTAGCA GCGAGGAAAT GAAATCCAGG CTCAGTAAAC TGCAGAGATT ACAGTTGCAA
    GTACCCCGGG GTCAGCGCCT GTTTGAATCT TTGCTTGTTT CAATGCCAGA CATGACAGTT
    ACTGATAATT TAAGGCTGGA AGACCTGCGC TACCAATGGA TGCTTTATAA ATCCAAATTA
    AGAGATGCTG GAAGCTCATC GGGCATAAAT ACATCTGAAG AGCTAAGAGG AATAACAAAG
    AAGTCATCTG GGGGACTGTG CTCCTTCCTG CACCGTGTGT GCTGTGCCGC ACTTCCACTC
    CAGTTACTCC TTCTATTACT TCTCCTCTTG GCATTGCTTA TTCTTCTGAT GCAGGAGAGC
    CAAAACTGCG CCCTTTCCAA CAACTTTGCC CGCTCCTTCA ACCTGATGCT AAAATACGAA
    AAACCTCCTC CAACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004917143.1
    CDS363..4100
    Translation

    Target ORF information:

    RefSeq Version XM_004917086.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004917086.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAGC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGATTCTG AAGAATTTGG ACCAAGTGGA ATTGTGGGCA GAATTGTCTA TGACACCAGT
    CCCCAAAGCT TAACATTAAG ATCAGAGAAA AGTCGGATAC CACAAAGGAT AAACAAAACA
    ACCAGTTTGC TCCAAGAAAA GGATCCATCC TCTACAACAG CCTCTGATAA GAGTTTTGTC
    TCTCTGGAAG TAAATACTAG CATGGCCGGT GACTCCAGTC CATCCAGGAT AACTGTGTGG
    TCAGACAAAA GCCGAATACC AATGAGGATA AACCAAACCT CCAGTTCCAG TGTTCTGCCA
    GAAAACTGTT CACCCTTCAC ACCACAAGAG TCTGGGATGA GTCCAAGAAC TGTGAAGAGT
    GGACTTTTGA ATGACACCAC GCCTCCCGAT GTGGCAGTCA GATCAGACAC TAGTCAGCCG
    CAAAAAAGGA GAACGAAAGC ATCTAGTGCC TCTGACCGCA GTAACTCAGG TAGTGGTCAA
    ACACTTCTAG GGAACAAGCA GGCATCCAGT GTTCTTCAAG TTGCTGATCC ACTTATCCCT
    TCAACAGAGG CTGGGGAAAG TTCAAGAACT TATACTGGAA GTATAGAAGA AAATTATTTA
    GTGAGCACAC CAGAAGAGTT TGGAGAGAGT TCAAGCTCTT TGACGATAAC TACTGGATCC
    CTGGCAGACA ACCTGAAGAA CCCAGTGAAA CCAGATTCTC AGTACAATAT GGTTTCTAGG
    AGAAGAAATA AGAAAGGTGT CAGCCTGGCA GAATCTGTGG ATTTTGTGGA TTCGTCTTAT
    GTGCAAAGTA AAGAAGAGAC AAAGACAGAG AAAGGTGATG GTCACATGAA TCTGCTGAAG
    GAATTTGAGC TGTGGTTGCA GGAAGAGAAT TCCAAACTAA ACAAAATCTG TAGCACAAAA
    GCATCTAGCA GCGAGGAAAT GAAATCCAGG CTCAGTAAAC TGCAGAGATT ACAGTTGCAA
    GTACCCCGGG GTCAGCGCCT GTTTGAATCT TTGCTTGTTT CAATGCCAGA CATGACAGTT
    ACTGATAATT TAAGGCTGGA AGACCTGCGC TACCAATGGA TGCTTTATAA ATCCAAATTA
    AGAGATGCTG GAAGCTCATC GGGCATAAAT ACATCTGAAG AGCTAAGAGG AATAACAAAG
    AAGTCATCTG GGGGACTGTG CTCCTTCCTG CACCGTGTGT GCTGTGCCGC ACTTCCACTC
    CAGTTACTCC TTCTATTACT TCTCCTCTTG GCATTGCTTA TTCTTCTGAT GCAGGAGAGC
    CAAAACTGCG CCCTTTCCAA CAACTTTGCC CGCTCCTTCA ACCTGATGCT AAAATACGAA
    AAACCTCCTC CAACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa34169
    Clone ID Related Accession (Same CDS sequence) XM_012969379.2
    Accession Version XM_012969379.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAGC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGAAATTG TCTATGACAC CAGTCCCCAA AGCTTAACAT TAAGATCAGA GAAAAGTCGG
    ATACCACAAA GGATAAACAA AACAACCAGT TTGCTCCAAG AAAAGGATCC ATCCTCTACA
    ACAGCCTCTG ATAAGAGTTT TGTCTCTCTG GAAGTAAATA CTAGCATGGC CGGTGACTCC
    AGTCCATCCA GGATAACTGT GTGGTCAGAC AAAAGCCGAA TACCAATGAG GATAAACCAA
    ACCTCCAGTT CCAGTGTTCT GCCAGAAAAC TGTTCACCCT TCACACCACA AGAGTCTGGG
    ATGAGTCCAA GAACTGTGAA GAGTGGACTT TTGAATGACA CCACGCCTCC CGATGTGGCA
    GTCAGATCAG ACACTAGTCA GCCGCAAAAA AGGAGAACGA AAGCATCTAG GTTAGAGAGC
    CCACATCCAA CTATATATTT TGATGCACAT TTCTGCAGTG CCTCTGACCG CAGTAACTCA
    GGTAGTGGTC AAACACTTCT AGGGAACAAG CAGGCATCCA GTGTTCTTCA AGTTGCTGAT
    CCACTTATCC CTTCAACAGA GGCTGGGGAA AGTTCAAGAA CTTATACTGG AAGTATAGAA
    GAAAATTATT TAGTGAGCAC ACCAGAAGAG TTTGGAGAGA GTTCAAGCTC TTTGACGATA
    ACTACTGGAT CCCTGGCAGA CAACCTGAAG AACCCAGTGA AACCAGATTC TCAGTACAAT
    ATGGTTTCTA GGAGAAGAAA TAAGAAAGGT GTCAGCCTGG CAGAATCTGT GGATTTTGTG
    GATTCGTCTT ATGTGCAAAG TAAAGAAGAG ACAAAGACAG AGAAAGGTGA TGGTCACATG
    AATCTGCTGA AGGAATTTGA GCTGTGGTTG CAGGAAGAGA ATTCCAAACT AAACAAAATC
    TGTAGCACAA AAGCATCTAG CAGCGAGGAA ATGAAATCCA GGCTCAGTAA ACTGCAGAGA
    TTACAGTTGC AAGTACCCCG GGGTCAGCGC CTGTTTGAAT CTTTGCTTGT TTCAATGCCA
    GACATGACAG TTACTGATAA TTTAAGGCTG GAAGACCTGC GCTACCAATG GATGCTTTAT
    AAATCCAAAT TAAGAGATGC TGGAAGCTCA TCGGGCATAA ATACATCTGA AGAGCTAAGA
    GGAATAACAA AGAAGTCATC TGGGGGACTG TGCTCCTTCC TGCACCGTGT GTGCTGTGCC
    GCACTTCCAC TCCAGTTACT CCTTCTATTA CTTCTCCTCT TGGCATTGCT TATTCTTCTG
    ATGCAGGAGA GCCAAAACTG CGCCCTTTCC AACAACTTTG CCCGCTCCTT CAACCTGATG
    CTAAAATACG AAAAACCTCC TCCAACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824833.1
    CDS364..4113
    Translation

    Target ORF information:

    RefSeq Version XM_012969379.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969379.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGGT TGCTGGAGGA GAGTGCTAGT ATTGAAGAAA AACTGGGTAT TCTGCAGACA
    AAGAAGGAGC GAATCAGTGG GGTGCTTGGT GAACAGAAGG CAGAAACCTT TTTTACTGAG
    GTGGCATTTG CTTCAAGGGA AAGGGATTCT CTCCATGCTG TTCTATTGGA GAAAAAGAAA
    ATTTTACTGA GTTTGGCTTC CCAGAAGAAA GAATTTGATG CTGCTTTTGA ACAGTTGCGG
    AAGAAGGTGT TGACTTTAAG AATTAAAGCT GGCAAAGAAA GTGAATTACA GCCAGACCTT
    GTAGGGAAAG AAACACAACT GCAGAGATTT CAGATGTTTA AGGATGATTT GTTAAAACTG
    GAACCTGAAT TTAAAGAGGT AGGGTCAATG GCTGAGTCAA GTCCCACCCT CAAGCTTAAA
    GTCAGCCAGC TGTCTTCAGA ATACCTTACT TTACACAAGT CACTTGAGAT TAAAATACGA
    AAAAGCAAAG CATACATTAA TGACCATCGG ATATTTAATG AGAAGCTGCT AGAGCTTCAG
    CGCTGGCTTA TGGTGACAAA GCAGGAGTTG GAATCATTTC AGGATAGTGA TGGGCATTGG
    GATTTTGAAT CCCGAGAGAA GGAAATACAG ATATTGTTGT CTGAGGTACC AGAAAAAGAT
    ATCCAGCTAC AACAGGTAGA ATCTCAAGGT TTGCTAGTGA TGGAGACCTC CTCTCCAGAA
    GGAGCTGCCC ACATTCAGAC TGAGCTGAGG GAGCTTAATG CCTCCTGGCT AAATCTGAAA
    CTACTCTGGG GGGCACTGGC AAGCGCGTTT AAAGACAGAG AGTTCCAGAG ACTGGCACTA
    CTGCCACCTA AAAGTACATC TGAGGACCAC CTACATCAGA CAACTGACCT CCTCCTGAAT
    ATGGAACAAT CCTGGACTTC ATCTCAGTCT GATAATAGCA ACAATGGCAT GAACAACAGT
    AATGAAATTG TCTATGACAC CAGTCCCCAA AGCTTAACAT TAAGATCAGA GAAAAGTCGG
    ATACCACAAA GGATAAACAA AACAACCAGT TTGCTCCAAG AAAAGGATCC ATCCTCTACA
    ACAGCCTCTG ATAAGAGTTT TGTCTCTCTG GAAGTAAATA CTAGCATGGC CGGTGACTCC
    AGTCCATCCA GGATAACTGT GTGGTCAGAC AAAAGCCGAA TACCAATGAG GATAAACCAA
    ACCTCCAGTT CCAGTGTTCT GCCAGAAAAC TGTTCACCCT TCACACCACA AGAGTCTGGG
    ATGAGTCCAA GAACTGTGAA GAGTGGACTT TTGAATGACA CCACGCCTCC CGATGTGGCA
    GTCAGATCAG ACACTAGTCA GCCGCAAAAA AGGAGAACGA AAGCATCTAG GTTAGAGAGC
    CCACATCCAA CTATATATTT TGATGCACAT TTCTGCAGTG CCTCTGACCG CAGTAACTCA
    GGTAGTGGTC AAACACTTCT AGGGAACAAG CAGGCATCCA GTGTTCTTCA AGTTGCTGAT
    CCACTTATCC CTTCAACAGA GGCTGGGGAA AGTTCAAGAA CTTATACTGG AAGTATAGAA
    GAAAATTATT TAGTGAGCAC ACCAGAAGAG TTTGGAGAGA GTTCAAGCTC TTTGACGATA
    ACTACTGGAT CCCTGGCAGA CAACCTGAAG AACCCAGTGA AACCAGATTC TCAGTACAAT
    ATGGTTTCTA GGAGAAGAAA TAAGAAAGGT GTCAGCCTGG CAGAATCTGT GGATTTTGTG
    GATTCGTCTT ATGTGCAAAG TAAAGAAGAG ACAAAGACAG AGAAAGGTGA TGGTCACATG
    AATCTGCTGA AGGAATTTGA GCTGTGGTTG CAGGAAGAGA ATTCCAAACT AAACAAAATC
    TGTAGCACAA AAGCATCTAG CAGCGAGGAA ATGAAATCCA GGCTCAGTAA ACTGCAGAGA
    TTACAGTTGC AAGTACCCCG GGGTCAGCGC CTGTTTGAAT CTTTGCTTGT TTCAATGCCA
    GACATGACAG TTACTGATAA TTTAAGGCTG GAAGACCTGC GCTACCAATG GATGCTTTAT
    AAATCCAAAT TAAGAGATGC TGGAAGCTCA TCGGGCATAA ATACATCTGA AGAGCTAAGA
    GGAATAACAA AGAAGTCATC TGGGGGACTG TGCTCCTTCC TGCACCGTGT GTGCTGTGCC
    GCACTTCCAC TCCAGTTACT CCTTCTATTA CTTCTCCTCT TGGCATTGCT TATTCTTCTG
    ATGCAGGAGA GCCAAAACTG CGCCCTTTCC AACAACTTTG CCCGCTCCTT CAACCTGATG
    CTAAAATACG AAAAACCTCC TCCAACTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa34170
    Clone ID Related Accession (Same CDS sequence) XM_012969380.2
    Accession Version XM_012969380.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3603bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nesprin-3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGTT TGGCTTCCCA GAAGAAAGAA TTTGATGCTG CTTTTGAACA GTTGCGGAAG
    AAGGTGTTGA CTTTAAGAAT TAAAGCTGGC AAAGAAAGTG AATTACAGCC AGACCTTGTA
    GGGAAAGAAA CACAACTGCA GAGATTTCAG ATGTTTAAGG ATGATTTGTT AAAACTGGAA
    CCTGAATTTA AAGAGGTAGG GTCAATGGCT GAGTCAAGTC CCACCCTCAA GCTTAAAGTC
    AGCCAGCTGT CTTCAGAATA CCTTACTTTA CACAAGTCAC TTGAGATTAA AATACGAAAA
    AGCAAAGCAT ACATTAATGA CCATCGGATA TTTAATGAGA AGCTGCTAGA GCTTCAGCGC
    TGGCTTATGG TGACAAAGCA GGAGTTGGAA TCATTTCAGG ATAGTGATGG GCATTGGGAT
    TTTGAATCCC GAGAGAAGGA AATACAGATA TTGTTGTCTG AGGTACCAGA AAAAGATATC
    CAGCTACAAC AGGTAGAATC TCAAGGTTTG CTAGTGATGG AGACCTCCTC TCCAGAAGGA
    GCTGCCCACA TTCAGACTGA GCTGAGGGAG CTTAATGCCT CCTGGCTAAA TCTGAAACTA
    CTCTGGGGGG CACTGGCAAG CGCGTTTAAA GACAGAGAGT TCCAGAGACT GGCACTACTG
    CCACCTAAAA GTACATCTGA GGACCACCTA CATCAGACAA CTGACCTCCT CCTGAATATG
    GAACAATCCT GGACTTCATC TCAGTCTGAT AATAGCAACA ATGGCATGAA CAACAGTAAT
    GATTCTGAAG AATTTGGACC AAGTGGAATT GTGGGCAGAA TTGTCTATGA CACCAGTCCC
    CAAAGCTTAA CATTAAGATC AGAGAAAAGT CGGATACCAC AAAGGATAAA CAAAACAACC
    AGTTTGCTCC AAGAAAAGGA TCCATCCTCT ACAACAGCCT CTGATAAGAG TTTTGTCTCT
    CTGGAAGTAA ATACTAGCAT GGCCGGTGAC TCCAGTCCAT CCAGGATAAC TGTGTGGTCA
    GACAAAAGCC GAATACCAAT GAGGATAAAC CAAACCTCCA GTTCCAGTGT TCTGCCAGAA
    AACTGTTCAC CCTTCACACC ACAAGAGTCT GGGATGAGTC CAAGAACTGT GAAGAGTGGA
    CTTTTGAATG ACACCACGCC TCCCGATGTG GCAGTCAGAT CAGACACTAG TCAGCCGCAA
    AAAAGGAGAA CGAAAGCATC TAGGTTAGAG AGCCCACATC CAACTATATA TTTTGATGCA
    CATTTCTGCA GTGCCTCTGA CCGCAGTAAC TCAGGTAGTG GTCAAACACT TCTAGGGAAC
    AAGCAGGCAT CCAGTGTTCT TCAAGTTGCT GATCCACTTA TCCCTTCAAC AGAGGCTGGG
    GAAAGTTCAA GAACTTATAC TGGAAGTATA GAAGAAAATT ATTTAGTGAG CACACCAGAA
    GAGTTTGGAG AGAGTTCAAG CTCTTTGACG ATAACTACTG GATCCCTGGC AGACAACCTG
    AAGAACCCAG TGAAACCAGA TTCTCAGTAC AATATGGTTT CTAGGAGAAG AAATAAGAAA
    GGTGTCAGCC TGGCAGAATC TGTGGATTTT GTGGATTCGT CTTATGTGCA AAGTAAAGAA
    GAGACAAAGA CAGAGAAAGG TGATGGTCAC ATGAATCTGC TGAAGGAATT TGAGCTGTGG
    TTGCAGGAAG AGAATTCCAA ACTAAACAAA ATCTGTAGCA CAAAAGCATC TAGCAGCGAG
    GAAATGAAAT CCAGGCTCAG TAAACTGCAG AGATTACAGT TGCAAGTACC CCGGGGTCAG
    CGCCTGTTTG AATCTTTGCT TGTTTCAATG CCAGACATGA CAGTTACTGA TAATTTAAGG
    CTGGAAGACC TGCGCTACCA ATGGATGCTT TATAAATCCA AATTAAGAGA TGCTGGAAGC
    TCATCGGGCA TAAATACATC TGAAGAGCTA AGAGGAATAA CAAAGAAGTC ATCTGGGGGA
    CTGTGCTCCT TCCTGCACCG TGTGTGCTGT GCCGCACTTC CACTCCAGTT ACTCCTTCTA
    TTACTTCTCC TCTTGGCATT GCTTATTCTT CTGATGCAGG AGAGCCAAAA CTGCGCCCTT
    TCCAACAACT TTGCCCGCTC CTTCAACCTG ATGCTAAAAT ACGAAAAACC TCCTCCAACT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012824834.1
    CDS363..3965
    Translation

    Target ORF information:

    RefSeq Version XM_012969380.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin repeat containing, nuclear envelope family member 3 (syne3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969380.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACTGAAG TGCCGCAGAG CGAATTTGAG AGCAGCTTGG AGAATGCTGA AACCTGGATG 
    AAAAATATCC ATGAGAGACT GAAGATCAAT GATAACACTG AAGGGCCCAG ATCTGCCCTG
    GAGGCCAGGC TCAGGGAGAC AGAGAAAATC TGTGCTCTGG AGCCTGAAGG CAAATTACTT
    TTGGACATGG TGCTGATGGC AGCAGAAAAT CTTCTCAGCA AGAGTAGTGA GGAAGAGAAA
    CACGAGGTTC ACATCAAGCT AGGGAAAATA AAGAGCATAT TTGAAGAAAC CACAGTTTAT
    ATGACACACT GCCACAGTCG CATTGAGTGG GTTTGGCTGC ACTGGAACGA GTACCTGAAA
    GCCAGAGATG AGTTTTATCT GTGGGTCCAT AACATGAAAC TGACCCTGGA ACCAGATGTG
    GAACTTCAGC TGGGCGTTAA AGAGAAGCGC TGGCAATATG AACAAGCACA AGTGCTGCTA
    AGAGATGTCA ACAACCAGTC ATGCCTGTTG GACAGACTAC TGGAGGAAGC CGCCTCTCTA
    TACAACAGGA TTGGAGACCC CAGTGTGGAT GAAACAGTGC AGAGAGAAAT GATGGCTGAA
    TACAAACAAA TAAAGAAGAA AGCCAAGGAA AGGGCAAGTC ATCTTGAAAA TATGTTGAAA
    GAACATGAAG CTTATGATAG GGATGTCAAT GAATTCCGGT CTTGGCTGAC CAGTGTGATT
    GAAAAGCTGA AGTGTTGTGT TGGAGAGGCC ATGGAATCAA CAGAATACAG GCTCAGTATG
    CTGCAGGAAA TCAGCAAAGA GGTACACAGT GGTGGTAAAA AGTTGGAAGA CCTTGAGGGC
    AAGTCACTTG AAGTGATAAA GAACACATCC CCTCTAGGTG CGGAGAATAT CTGCAAGGAG
    CTGGAGGAGT TGCACAAAGC TCTAGCAGAT CTGAAACGCA TGAATGATGA TGAAGAACAG
    AGTCTTCTCA AGGCTCATAA TTCTGAAAAT GCCTTCCTAC TTTTGGCTAG GCAACTAGAA
    GCCAACATCA ATGAGTTCCG AAAAGCCATC CAGAGACTGG AGGAAAGTCT GGAATCAGGA
    GAAAGGGTGA AGAGTGAAGA TGAACTGGTA GCTCTGTGGA AAACATTAAA CGCCACCAAG
    TCTGCTTTAG CAGCAGAGGA AACAAAAGGT GAAGGGGTAA AGGTTCAGCT GAAGGAACTC
    TTCAAGTTCT CCAAGGATGT CCAGCCGCTT TCTGATGAAG TCATTGCTGC AATTCGAGAA
    TACCAAAGGG CTAAAAGCAA AGCCTTCAAG CTGAGTACTG AGACTGAATC GGCACTCATA
    CAGCACTTCC AGATTCCGCT TCTGGAATTT CACCATTGGA AACGCATAGG TGACAGAGTC
    CTGGATACCA CATCAACTCC TGTGAGCGAT TATGCTCTGA ACCATGACTG CTTGCTACAG
    ATTGAGAGTT TGGCTTCCCA GAAGAAAGAA TTTGATGCTG CTTTTGAACA GTTGCGGAAG
    AAGGTGTTGA CTTTAAGAAT TAAAGCTGGC AAAGAAAGTG AATTACAGCC AGACCTTGTA
    GGGAAAGAAA CACAACTGCA GAGATTTCAG ATGTTTAAGG ATGATTTGTT AAAACTGGAA
    CCTGAATTTA AAGAGGTAGG GTCAATGGCT GAGTCAAGTC CCACCCTCAA GCTTAAAGTC
    AGCCAGCTGT CTTCAGAATA CCTTACTTTA CACAAGTCAC TTGAGATTAA AATACGAAAA
    AGCAAAGCAT ACATTAATGA CCATCGGATA TTTAATGAGA AGCTGCTAGA GCTTCAGCGC
    TGGCTTATGG TGACAAAGCA GGAGTTGGAA TCATTTCAGG ATAGTGATGG GCATTGGGAT
    TTTGAATCCC GAGAGAAGGA AATACAGATA TTGTTGTCTG AGGTACCAGA AAAAGATATC
    CAGCTACAAC AGGTAGAATC TCAAGGTTTG CTAGTGATGG AGACCTCCTC TCCAGAAGGA
    GCTGCCCACA TTCAGACTGA GCTGAGGGAG CTTAATGCCT CCTGGCTAAA TCTGAAACTA
    CTCTGGGGGG CACTGGCAAG CGCGTTTAAA GACAGAGAGT TCCAGAGACT GGCACTACTG
    CCACCTAAAA GTACATCTGA GGACCACCTA CATCAGACAA CTGACCTCCT CCTGAATATG
    GAACAATCCT GGACTTCATC TCAGTCTGAT AATAGCAACA ATGGCATGAA CAACAGTAAT
    GATTCTGAAG AATTTGGACC AAGTGGAATT GTGGGCAGAA TTGTCTATGA CACCAGTCCC
    CAAAGCTTAA CATTAAGATC AGAGAAAAGT CGGATACCAC AAAGGATAAA CAAAACAACC
    AGTTTGCTCC AAGAAAAGGA TCCATCCTCT ACAACAGCCT CTGATAAGAG TTTTGTCTCT
    CTGGAAGTAA ATACTAGCAT GGCCGGTGAC TCCAGTCCAT CCAGGATAAC TGTGTGGTCA
    GACAAAAGCC GAATACCAAT GAGGATAAAC CAAACCTCCA GTTCCAGTGT TCTGCCAGAA
    AACTGTTCAC CCTTCACACC ACAAGAGTCT GGGATGAGTC CAAGAACTGT GAAGAGTGGA
    CTTTTGAATG ACACCACGCC TCCCGATGTG GCAGTCAGAT CAGACACTAG TCAGCCGCAA
    AAAAGGAGAA CGAAAGCATC TAGGTTAGAG AGCCCACATC CAACTATATA TTTTGATGCA
    CATTTCTGCA GTGCCTCTGA CCGCAGTAAC TCAGGTAGTG GTCAAACACT TCTAGGGAAC
    AAGCAGGCAT CCAGTGTTCT TCAAGTTGCT GATCCACTTA TCCCTTCAAC AGAGGCTGGG
    GAAAGTTCAA GAACTTATAC TGGAAGTATA GAAGAAAATT ATTTAGTGAG CACACCAGAA
    GAGTTTGGAG AGAGTTCAAG CTCTTTGACG ATAACTACTG GATCCCTGGC AGACAACCTG
    AAGAACCCAG TGAAACCAGA TTCTCAGTAC AATATGGTTT CTAGGAGAAG AAATAAGAAA
    GGTGTCAGCC TGGCAGAATC TGTGGATTTT GTGGATTCGT CTTATGTGCA AAGTAAAGAA
    GAGACAAAGA CAGAGAAAGG TGATGGTCAC ATGAATCTGC TGAAGGAATT TGAGCTGTGG
    TTGCAGGAAG AGAATTCCAA ACTAAACAAA ATCTGTAGCA CAAAAGCATC TAGCAGCGAG
    GAAATGAAAT CCAGGCTCAG TAAACTGCAG AGATTACAGT TGCAAGTACC CCGGGGTCAG
    CGCCTGTTTG AATCTTTGCT TGTTTCAATG CCAGACATGA CAGTTACTGA TAATTTAAGG
    CTGGAAGACC TGCGCTACCA ATGGATGCTT TATAAATCCA AATTAAGAGA TGCTGGAAGC
    TCATCGGGCA TAAATACATC TGAAGAGCTA AGAGGAATAA CAAAGAAGTC ATCTGGGGGA
    CTGTGCTCCT TCCTGCACCG TGTGTGCTGT GCCGCACTTC CACTCCAGTT ACTCCTTCTA
    TTACTTCTCC TCTTGGCATT GCTTATTCTT CTGATGCAGG AGAGCCAAAA CTGCGCCCTT
    TCCAACAACT TTGCCCGCTC CTTCAACCTG ATGCTAAAAT ACGAAAAACC TCCTCCAACT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.