wnk2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following wnk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa57686 XM_031901291.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa57687 XM_031901292.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa57682 XM_031901287.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa57683 XM_031901288.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa57684 XM_031901289.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OXa57685 XM_031901290.1
Latest version!
Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa57686
    Clone ID Related Accession (Same CDS sequence) XM_031901291.1
    Accession Version XM_031901291.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5865bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
    GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG
    CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG
    CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT
    TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC
    GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA
    GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG
    AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG
    GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC
    GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG
    GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA
    AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC
    CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC
    GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG
    GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC
    CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC
    ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC
    TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA
    CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG
    TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC
    GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC
    GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC
    TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA
    TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA
    GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA
    CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA
    ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT
    GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC
    CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT
    CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC
    CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT
    CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT
    CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT
    ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG
    ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT
    GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG
    TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA
    CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG
    GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT
    ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG
    GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA
    ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA
    ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA
    GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT
    GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC
    AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA
    AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG
    ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG
    ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT
    GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG
    GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG
    AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT
    ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA
    GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC
    TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC
    ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA
    TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC
    TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA
    ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA
    GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA
    GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA
    CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA
    AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC
    ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA
    TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT
    ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC
    ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA
    GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC
    TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT
    GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT
    GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC
    AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA
    AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA
    GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC
    CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG
    TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT
    CGCTCTGGAA AGGCCAGTAC CCAAAGCCCC ACCTCTCCCA ACGGCCAAGC CATGAAAATC
    CCTTCAATAA ACGTGACCTC TTTCCATTCG CAGTCGTCCT ATATGAGCAG TGATAACGAC
    TCGGAGTTTG AAGATGCCGA CATGAAGAAG GAACTGCAGA GCCTCCGGGA GAAGCACATG
    AAGGAAATAA CAGAACTGCA ATCGCAGCAG AAGAAGGAGA TTGAGGCCCT TTACCTCCGG
    CTGGGGAAGC CCCTCCCACC CAATGTGGGA TTCCTGCACG CAGCACCCCC CAGTGGCCGC
    CGGAGGAGGA TCAGCAAAAA TAAGCTGAAA ACTGGGAAAC TGCTCAATCC GCTGGTGCAG
    CAGCTGAAGA ATTCGACTAC CAGTCCAAGC CGCATTTCCC CAGAATGCAA TGGTTCCCCT
    GCTAAGGATC CTGCAAAGTC TCTGGCAGAC TGCACCGCAG GAACCTCGCC AAGCCGTGCC
    GTACAAACGC AGCAGCCTTG CTCTGTGAAA GTCACCATGT CGTCCGATAA TATCTGCTCT
    GGGGTCACCC GGGACGGTGT CGATGTGCAG AGCAATTCAA GCCAAGGCTG GACGGTTTAC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTCTGTC CATCTGGTCC ACTCTGAAAC GCCTGTGTCT AGGCAAAGAT
    CACAGTAGTA GATCTTCGAC AAGCAGCCTC GCAACCGCCC CCGAGGCCTC GGCGCTGCCC
    CCGCTGAACG CACAAATCCA GCCGCAGGCC AACAACAGCA ACAACAACAA AAAGGGGACG
    TTTACGGATG ACCTTCACAA ATTGGTGGAT GAGTGGGCCT GCAAAACCGT CGGCGTGGCA
    CAATTCAAAC CCTCATTAAA CCAATTGAAG CAGTACCAGC AGCGGCAAGA TCTGGAGCCC
    AAGTCTGTGC CCAGCGACCT GTGTGACATG AGCCGTGCCG TGCCAGAGCC CAGGAAGAGA
    TACAGCATTC CCATGGCTGC CCCTCCGGCT GTACCACTGG CTCCCCTCCT CTCTGCCGCT
    GTCCCAGCAA CTGTTCCAGG AGCCGTGCAG CCGGGTACCT TTGTCATGCC CTTTTGTCCG
    TACGCGGCAC CAATGTACGG CATGCCCTGG GCAGGAGCGG CTGAGCTTGG GACTGCAGGA
    AATCAGATCA TGGCCCAAGT AGCCCCCATA GGCAACCAAA TATTCCCTGT GGCACTGCAG
    CACCCAGGTT TGCACCCTGC TGGATCCAAC ATGCGGATTA CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757151.1
    CDS427..6291
    Translation

    Target ORF information:

    RefSeq Version XM_031901291.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901291.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
    GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG
    CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG
    CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT
    TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC
    GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA
    GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG
    AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG
    GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC
    GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG
    GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA
    AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC
    CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC
    GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG
    GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC
    CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC
    ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC
    TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA
    CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG
    TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC
    GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC
    GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC
    TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA
    TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA
    GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA
    CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA
    ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT
    GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC
    CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT
    CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC
    CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT
    CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT
    CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT
    ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG
    ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT
    GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG
    TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA
    CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG
    GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT
    ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG
    GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA
    ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA
    ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA
    GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT
    GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC
    AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA
    AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG
    ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG
    ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT
    GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG
    GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG
    AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT
    ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA
    GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC
    TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC
    ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA
    TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC
    TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA
    ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA
    GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA
    GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA
    CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA
    AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC
    ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA
    TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT
    ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC
    ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA
    GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC
    TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT
    GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT
    GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC
    AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA
    AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA
    GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC
    CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG
    TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT
    CGCTCTGGAA AGGCCAGTAC CCAAAGCCCC ACCTCTCCCA ACGGCCAAGC CATGAAAATC
    CCTTCAATAA ACGTGACCTC TTTCCATTCG CAGTCGTCCT ATATGAGCAG TGATAACGAC
    TCGGAGTTTG AAGATGCCGA CATGAAGAAG GAACTGCAGA GCCTCCGGGA GAAGCACATG
    AAGGAAATAA CAGAACTGCA ATCGCAGCAG AAGAAGGAGA TTGAGGCCCT TTACCTCCGG
    CTGGGGAAGC CCCTCCCACC CAATGTGGGA TTCCTGCACG CAGCACCCCC CAGTGGCCGC
    CGGAGGAGGA TCAGCAAAAA TAAGCTGAAA ACTGGGAAAC TGCTCAATCC GCTGGTGCAG
    CAGCTGAAGA ATTCGACTAC CAGTCCAAGC CGCATTTCCC CAGAATGCAA TGGTTCCCCT
    GCTAAGGATC CTGCAAAGTC TCTGGCAGAC TGCACCGCAG GAACCTCGCC AAGCCGTGCC
    GTACAAACGC AGCAGCCTTG CTCTGTGAAA GTCACCATGT CGTCCGATAA TATCTGCTCT
    GGGGTCACCC GGGACGGTGT CGATGTGCAG AGCAATTCAA GCCAAGGCTG GACGGTTTAC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTCTGTC CATCTGGTCC ACTCTGAAAC GCCTGTGTCT AGGCAAAGAT
    CACAGTAGTA GATCTTCGAC AAGCAGCCTC GCAACCGCCC CCGAGGCCTC GGCGCTGCCC
    CCGCTGAACG CACAAATCCA GCCGCAGGCC AACAACAGCA ACAACAACAA AAAGGGGACG
    TTTACGGATG ACCTTCACAA ATTGGTGGAT GAGTGGGCCT GCAAAACCGT CGGCGTGGCA
    CAATTCAAAC CCTCATTAAA CCAATTGAAG CAGTACCAGC AGCGGCAAGA TCTGGAGCCC
    AAGTCTGTGC CCAGCGACCT GTGTGACATG AGCCGTGCCG TGCCAGAGCC CAGGAAGAGA
    TACAGCATTC CCATGGCTGC CCCTCCGGCT GTACCACTGG CTCCCCTCCT CTCTGCCGCT
    GTCCCAGCAA CTGTTCCAGG AGCCGTGCAG CCGGGTACCT TTGTCATGCC CTTTTGTCCG
    TACGCGGCAC CAATGTACGG CATGCCCTGG GCAGGAGCGG CTGAGCTTGG GACTGCAGGA
    AATCAGATCA TGGCCCAAGT AGCCCCCATA GGCAACCAAA TATTCCCTGT GGCACTGCAG
    CACCCAGGTT TGCACCCTGC TGGATCCAAC ATGCGGATTA CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57687
    Clone ID Related Accession (Same CDS sequence) XM_031901292.1
    Accession Version XM_031901292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
    GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG
    CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG
    CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT
    TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC
    GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA
    GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG
    AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG
    GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC
    GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG
    GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA
    AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC
    CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC
    GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG
    GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC
    CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC
    ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC
    TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA
    CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG
    TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC
    GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC
    GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC
    TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA
    TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA
    GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA
    CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA
    ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT
    GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC
    CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT
    CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC
    CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT
    CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT
    CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT
    ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG
    ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT
    GGACAGGTTG TTCTGCCCCA ATATCCTCTG GATGTGGGAA CTGCCATGGG GGTTATGGTA
    CCTTCTACAA CAATGTCACC ACCAGTGCAG TTTCCAGCTG AATCTGCCCC TCTGCACCCA
    CCTCAGGAAC ATGTCACCCC GGTATCCTAT ATACACACAG ATGTACCTAT GACCCAACAG
    AGCACAACTC TTCAGTCTGA GGGTCATCAG CCCGAAACTA CACAGCCACC TGTCCATCAG
    AGTCAAACTC TCTACCAAGC CCCCCGGCCT TCTGTGGAGA CCAATGTAGA GGAGCCGACT
    CTGCAAGAGA ATCCAGTGAG TTCCGCACAA AGTTGTGATA ACTATGCAGG CTCAGATGCT
    GCGTCTGGTA GAGAGATGAG TGATGGTGGA GAAGGGGTTC ATGGGAAACA GGATGGAAAA
    CCAACCAAAA AGCATCACCG GAGGTCTTCC CGCACCCGCT CTCGCCAGGA GAAGGCCAAT
    AAACCAAAGC TGACTATTCT TAATGTGAGC AATACAGGTG ACAAGATGGT GGAATGCCAG
    TTGGAGACTC ACAACCATAA AATGGTGACT TTCAAATTTG ACTTGGATGG CGATGCACCA
    GATGAGATTG CCACGTACAT GGTGGAGAAT GGCTTTATTC TGCAGATGGA GAAGGAGGTG
    TTTATTGAGC AAATGAAGGA TGTTATGGAC AAAGCGGAAG ACATGTTGAG CGAAGACACT
    GAAGGGGAGA GAAGTTCAGA CCAAGCGGCG AGTCCTCAGA AAGTGGATTC TAATGGGAGA
    GAGACGAGGG AGGAAAACAC CCCAAATCAA ATGAAGACTC CGGTCTATCA GCAAAACGTT
    CTACATACTG GGAAGCGATG GTTTATTATA TGTCCAGTGG CCGAAACCAC CGGTTCCTCC
    CTGCCAGAAG TGCCCCAAAC TCCCTTAAAT ACCCAGAAGC CGACTGACGT TAAAGATCAG
    ACCGCCTGCG AGGATTCAGT ATTTGCCACA AATGCGGATA AGTTGGACTT TGCGGTGCCG
    CCGGGCATAC TGTGTGAGAG TGCCAGTGCC ATTCCTGCCT CAAGGACACA ATCTTCTTCT
    ACAATATCTT CCTCCGATCT GCAAACCCAA ACGGAACTGT CCTTTTCTGC TCTTCCTGAG
    TCTCTCTCCT TTCTCCAAGA AGAGGAACCG GTCCTGTTCA GCCCGCCGGC AGTTACGCAG
    CCTCTAATGG ACTCCATAAG CCATGAGGCC GGAGGGAGCC TGGCGCCTGT ATTCTCCGCA
    GCACAAGAAC CCGAGCAGAA ACCCAGTCCT ACAGATTCAG TAAATTCAAC AGAACCGCCA
    CGTGCAGCCT TACCCCTCTC CGACCACGAA CGACCGGCCG CTCAGCAAGA ACGGACTCTA
    GATCCACCCC CAGAGCGGAG TACATTGTAT GGATCCAGTA TTGGTTTCCC CGTTGTAAAC
    TCCATAAAAC CAGATGCCCC CTTGCCCATA CCCCCTTGCC AGCCACACCT TGATTCTGAA
    ATTTCCACCC CACTGAAACC AGTCCCAGTC CCCACTCAGT CTCTGGTTTT ACCAGTTGGC
    GGCACATCTT GCCAACAGCC CAGTATTACC GAGTCGGACG GGGAAGGCCC CCCGAGGGTA
    GAATTTACCG ACGATACCAT TAAAAGCTTA GATGAAAAGC TCCGCACTCT CTTGTACCAG
    GAATACACCC CGGCCAACGT TTCTGCCGGA ACCCCTGACA ATTGTTCCGG AGCAGAAAGC
    GGAGCAGAGA CAACGTTGGG CAACGGAGAA GAGATTGCTG AACACAGAAA CCTCTCCCAG
    TCTGTCTCTG CGCCACTAGA GAAAGCAACG GAACTGGGCT GGACTGTCGC AGAGAAAAGT
    TCTTGTGAGC TGTCAAACCA AAAGATTCAG GCTGCACCTC TGAACCATTC CCAGGGGTGG
    GATTCTGGCA AAGAAAGTGC AGTTGACTCT TCTTCCAAAA AGAGAGAGAC CCCTGACAAG
    TCCTCCAAGA CCATCGGCAG GTTCTCGGTT GTGAGTGCAG AAGATGATGC CCCAGTGACC
    GCTCCAAATT ACACGAGATT TTCAGCCCCG CCCGATGTCT ACCTGGATCA GGCTCCACCA
    CAACCAGCCA GGGCCTCTGT CCCTAGAGCT CACACCTCTT CCCCTATTAA CTCTCTGTTC
    TGTGGCCATG TTTCCAGTGA CTCTGGGGAT GATACCCCTC ACTCCAAGTC CAAAGAGGCT
    CAGCCGTCCA CCCCAAGTAA GGGGGGCACC AATGACTTCA TGAAGAAGGC CGCTGCTTTC
    TTGCATCGCT CTGGAAAGGC CAGTACCCAA AGCCCCACCT CTCCCAACGG CCAAGCCATG
    AAAATCCCTT CAATAAACGT GACCTCTTTC CATTCGCAGT CGTCCTATAT GAGCAGTGAT
    AACGACTCGG AGTTTGAAGA TGCCGACATG AAGAAGGAAC TGCAGAGCCT CCGGGAGAAG
    CACATGAAGG AAATAACAGA ACTGCAATCG CAGCAGAAGA AGGAGATTGA GGCCCTTTAC
    CTCCGGCTGG GGAAGCCCCT CCCACCCAAT GTGGGATTCC TGCACGCAGC ACCCCCCAGT
    GGCCGCCGGA GGAGGATCAG CAAAAATAAG CTGAAAACTG GGAAACTGCT CAATCCGCTG
    GTGCAGCAGC TGAAGAATTC GACTACCAGT CCAAGCCGCA TTTCCCCAGA ATGCAATGGT
    TCCCCTGCTA AGGATCCTGC AAAGTCTCTG GCAGACTGCA CCGCAGGAAC CTCGCCAAGC
    CGTGCCGTAC AAACGCAGCA GCCTTGCTCT GTGAAAGTCA CCATGTCGTC CGATAATATC
    TGCTCTGGGG TCACCCGGGA CGGTGTCGAT GTGCAGAGCA ATTCAAGCCA AGGCTGGACG
    GTTTACCACC AAACGTCTGA GAGAGTGACC TATAAATCTA GTAGCAAACC TCGTACCAGA
    TTCCTCAGTG GACCTGTGTC TCTGTCCATC TGGTCCACTC TGAAACGCCT GTGTCTAGGC
    AAAGATCACA GTAGTAGATC TTCGACAAGC AGCCTCGCAA CCGCCCCCGA GGCCTCGGCG
    CTGCCCCCGC TGAACGCACA AATCCAGCCG CAGGCCAACA ACAGCAACAA CAACAAAAAG
    GGGACGTTTA CGGATGACCT TCACAAATTG GTGGATGAGT GGGCCTGCAA AACCGTCGGC
    GTGGCACAAT TCAAACCCTC ATTAAACCAA TTGAAGCAGT ACCAGCAGCG GCAAGATCTG
    GAGCCCAAGT CTGTGCCCAG CGACCTGTGT GACATGAGCC GTGCCGTGCC AGAGCCCAGG
    AAGAGATACA GCATTCCCAT GGCTGCCCCT CCGGCTGTAC CACTGGCTCC CCTCCTCTCT
    GCCGCTGTCC CAGCAACTGT TCCAGGAGCC GTGCAGCCGG GTACCTTTGT CATGCCCTTT
    TGTCCGTACG CGGCACCAAT GTACGGCATG CCCTGGGCAG GAGCGGCTGA GCTTGGGACT
    GCAGGAAATC AGATCATGGC CCAAGTAGCC CCCATAGGCA ACCAAATATT CCCTGTGGCA
    CTGCAGCACC CAGGTTTGCA CCCTGCTGGA TCCAACATGC GGATTACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757152.1
    CDS427..6117
    Translation

    Target ORF information:

    RefSeq Version XM_031901292.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
    GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG
    CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG
    CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT
    TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC
    GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA
    GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG
    AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG
    GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC
    GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG
    GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA
    AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC
    CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC
    GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG
    GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC
    CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC
    ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC
    TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA
    CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG
    TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC
    GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC
    GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC
    TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA
    TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA
    GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA
    CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA
    ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT
    GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC
    CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT
    CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC
    CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT
    CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT
    CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT
    ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG
    ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT
    GGACAGGTTG TTCTGCCCCA ATATCCTCTG GATGTGGGAA CTGCCATGGG GGTTATGGTA
    CCTTCTACAA CAATGTCACC ACCAGTGCAG TTTCCAGCTG AATCTGCCCC TCTGCACCCA
    CCTCAGGAAC ATGTCACCCC GGTATCCTAT ATACACACAG ATGTACCTAT GACCCAACAG
    AGCACAACTC TTCAGTCTGA GGGTCATCAG CCCGAAACTA CACAGCCACC TGTCCATCAG
    AGTCAAACTC TCTACCAAGC CCCCCGGCCT TCTGTGGAGA CCAATGTAGA GGAGCCGACT
    CTGCAAGAGA ATCCAGTGAG TTCCGCACAA AGTTGTGATA ACTATGCAGG CTCAGATGCT
    GCGTCTGGTA GAGAGATGAG TGATGGTGGA GAAGGGGTTC ATGGGAAACA GGATGGAAAA
    CCAACCAAAA AGCATCACCG GAGGTCTTCC CGCACCCGCT CTCGCCAGGA GAAGGCCAAT
    AAACCAAAGC TGACTATTCT TAATGTGAGC AATACAGGTG ACAAGATGGT GGAATGCCAG
    TTGGAGACTC ACAACCATAA AATGGTGACT TTCAAATTTG ACTTGGATGG CGATGCACCA
    GATGAGATTG CCACGTACAT GGTGGAGAAT GGCTTTATTC TGCAGATGGA GAAGGAGGTG
    TTTATTGAGC AAATGAAGGA TGTTATGGAC AAAGCGGAAG ACATGTTGAG CGAAGACACT
    GAAGGGGAGA GAAGTTCAGA CCAAGCGGCG AGTCCTCAGA AAGTGGATTC TAATGGGAGA
    GAGACGAGGG AGGAAAACAC CCCAAATCAA ATGAAGACTC CGGTCTATCA GCAAAACGTT
    CTACATACTG GGAAGCGATG GTTTATTATA TGTCCAGTGG CCGAAACCAC CGGTTCCTCC
    CTGCCAGAAG TGCCCCAAAC TCCCTTAAAT ACCCAGAAGC CGACTGACGT TAAAGATCAG
    ACCGCCTGCG AGGATTCAGT ATTTGCCACA AATGCGGATA AGTTGGACTT TGCGGTGCCG
    CCGGGCATAC TGTGTGAGAG TGCCAGTGCC ATTCCTGCCT CAAGGACACA ATCTTCTTCT
    ACAATATCTT CCTCCGATCT GCAAACCCAA ACGGAACTGT CCTTTTCTGC TCTTCCTGAG
    TCTCTCTCCT TTCTCCAAGA AGAGGAACCG GTCCTGTTCA GCCCGCCGGC AGTTACGCAG
    CCTCTAATGG ACTCCATAAG CCATGAGGCC GGAGGGAGCC TGGCGCCTGT ATTCTCCGCA
    GCACAAGAAC CCGAGCAGAA ACCCAGTCCT ACAGATTCAG TAAATTCAAC AGAACCGCCA
    CGTGCAGCCT TACCCCTCTC CGACCACGAA CGACCGGCCG CTCAGCAAGA ACGGACTCTA
    GATCCACCCC CAGAGCGGAG TACATTGTAT GGATCCAGTA TTGGTTTCCC CGTTGTAAAC
    TCCATAAAAC CAGATGCCCC CTTGCCCATA CCCCCTTGCC AGCCACACCT TGATTCTGAA
    ATTTCCACCC CACTGAAACC AGTCCCAGTC CCCACTCAGT CTCTGGTTTT ACCAGTTGGC
    GGCACATCTT GCCAACAGCC CAGTATTACC GAGTCGGACG GGGAAGGCCC CCCGAGGGTA
    GAATTTACCG ACGATACCAT TAAAAGCTTA GATGAAAAGC TCCGCACTCT CTTGTACCAG
    GAATACACCC CGGCCAACGT TTCTGCCGGA ACCCCTGACA ATTGTTCCGG AGCAGAAAGC
    GGAGCAGAGA CAACGTTGGG CAACGGAGAA GAGATTGCTG AACACAGAAA CCTCTCCCAG
    TCTGTCTCTG CGCCACTAGA GAAAGCAACG GAACTGGGCT GGACTGTCGC AGAGAAAAGT
    TCTTGTGAGC TGTCAAACCA AAAGATTCAG GCTGCACCTC TGAACCATTC CCAGGGGTGG
    GATTCTGGCA AAGAAAGTGC AGTTGACTCT TCTTCCAAAA AGAGAGAGAC CCCTGACAAG
    TCCTCCAAGA CCATCGGCAG GTTCTCGGTT GTGAGTGCAG AAGATGATGC CCCAGTGACC
    GCTCCAAATT ACACGAGATT TTCAGCCCCG CCCGATGTCT ACCTGGATCA GGCTCCACCA
    CAACCAGCCA GGGCCTCTGT CCCTAGAGCT CACACCTCTT CCCCTATTAA CTCTCTGTTC
    TGTGGCCATG TTTCCAGTGA CTCTGGGGAT GATACCCCTC ACTCCAAGTC CAAAGAGGCT
    CAGCCGTCCA CCCCAAGTAA GGGGGGCACC AATGACTTCA TGAAGAAGGC CGCTGCTTTC
    TTGCATCGCT CTGGAAAGGC CAGTACCCAA AGCCCCACCT CTCCCAACGG CCAAGCCATG
    AAAATCCCTT CAATAAACGT GACCTCTTTC CATTCGCAGT CGTCCTATAT GAGCAGTGAT
    AACGACTCGG AGTTTGAAGA TGCCGACATG AAGAAGGAAC TGCAGAGCCT CCGGGAGAAG
    CACATGAAGG AAATAACAGA ACTGCAATCG CAGCAGAAGA AGGAGATTGA GGCCCTTTAC
    CTCCGGCTGG GGAAGCCCCT CCCACCCAAT GTGGGATTCC TGCACGCAGC ACCCCCCAGT
    GGCCGCCGGA GGAGGATCAG CAAAAATAAG CTGAAAACTG GGAAACTGCT CAATCCGCTG
    GTGCAGCAGC TGAAGAATTC GACTACCAGT CCAAGCCGCA TTTCCCCAGA ATGCAATGGT
    TCCCCTGCTA AGGATCCTGC AAAGTCTCTG GCAGACTGCA CCGCAGGAAC CTCGCCAAGC
    CGTGCCGTAC AAACGCAGCA GCCTTGCTCT GTGAAAGTCA CCATGTCGTC CGATAATATC
    TGCTCTGGGG TCACCCGGGA CGGTGTCGAT GTGCAGAGCA ATTCAAGCCA AGGCTGGACG
    GTTTACCACC AAACGTCTGA GAGAGTGACC TATAAATCTA GTAGCAAACC TCGTACCAGA
    TTCCTCAGTG GACCTGTGTC TCTGTCCATC TGGTCCACTC TGAAACGCCT GTGTCTAGGC
    AAAGATCACA GTAGTAGATC TTCGACAAGC AGCCTCGCAA CCGCCCCCGA GGCCTCGGCG
    CTGCCCCCGC TGAACGCACA AATCCAGCCG CAGGCCAACA ACAGCAACAA CAACAAAAAG
    GGGACGTTTA CGGATGACCT TCACAAATTG GTGGATGAGT GGGCCTGCAA AACCGTCGGC
    GTGGCACAAT TCAAACCCTC ATTAAACCAA TTGAAGCAGT ACCAGCAGCG GCAAGATCTG
    GAGCCCAAGT CTGTGCCCAG CGACCTGTGT GACATGAGCC GTGCCGTGCC AGAGCCCAGG
    AAGAGATACA GCATTCCCAT GGCTGCCCCT CCGGCTGTAC CACTGGCTCC CCTCCTCTCT
    GCCGCTGTCC CAGCAACTGT TCCAGGAGCC GTGCAGCCGG GTACCTTTGT CATGCCCTTT
    TGTCCGTACG CGGCACCAAT GTACGGCATG CCCTGGGCAG GAGCGGCTGA GCTTGGGACT
    GCAGGAAATC AGATCATGGC CCAAGTAGCC CCCATAGGCA ACCAAATATT CCCTGTGGCA
    CTGCAGCACC CAGGTTTGCA CCCTGCTGGA TCCAACATGC GGATTACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57682
    Clone ID Related Accession (Same CDS sequence) XM_031901287.1
    Accession Version XM_031901287.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6345bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
    GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG
    CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG
    CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT
    TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC
    GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA
    GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG
    AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG
    GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC
    GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG
    GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA
    AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC
    CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC
    GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG
    GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC
    CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC
    ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC
    TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA
    CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG
    TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC
    GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC
    GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC
    TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA
    TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA
    GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA
    CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA
    ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT
    GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC
    CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT
    CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC
    CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT
    CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT
    CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT
    ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG
    ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT
    GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG
    TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA
    CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG
    CTTCCACCGT ACCCTCTCAT TCCAGCAGTT ACTCCTCTCG CAGGACTAGA GACTCTCCCT
    GGCGCCAGTC TCTCTGACTT TCCCGCTGTC TGTGTTACCC CATTACCTGC CCCCTGTCAC
    TACTTTCCCC CAGGCATTGT GGTGGCCAGT GTCCCATTGA CTCCTGCCAT AGCCCCACCC
    CTGCCTTTGC AAGCCATGAA TTCGCAGTTG GTACTGTCCT ATGAGACTAA CATAGTCCCA
    AATGTGCCAG TGGGCACGGT TCCATTGCTA GCAGCAGCCG GTGTCCCAAC GTTACCCACC
    CCACAGGCTG TTTCCCAGCA TCCCCAAGTT CAGCAGTTCC AAACATCCTA CCCACCCCTC
    ATTCAGAGTG ATGTTCCCTC TCAAAATGTC TCCCATCAAG CAACTGTTCC ACCTCCTCAA
    ACCCACCATC CGAGCATCCC TCATCCCTCT GACCAGGCCT CGGCCGGGGC ATGTAACCAG
    GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT
    ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG
    GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA
    ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA
    ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA
    GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT
    GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC
    AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA
    AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG
    ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG
    ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT
    GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG
    GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG
    AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT
    ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA
    GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC
    TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC
    ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA
    TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC
    TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA
    ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA
    GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA
    GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA
    CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA
    AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC
    ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA
    TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT
    ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC
    ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA
    GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC
    TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT
    GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT
    GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC
    AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA
    AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA
    GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC
    CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG
    TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT
    CGCTCTGGAA AGGCCAGTAC CCAAAGCCCC ACCTCTCCCA ACGGCCAAGC CATGAAAATC
    CCTTCAATAA ACGTGACCTC TTTCCATTCG CAGTCGTCCT ATATGAGCAG TGATAACGAC
    TCGGAGTTTG AAGATGCCGA CATGAAGAAG GAACTGCAGA GCCTCCGGGA GAAGCACATG
    AAGGAAATAA CAGAACTGCA ATCGCAGCAG AAGAAGGAGA TTGAGGCCCT TTACCTCCGG
    CTGGGGAAGC CCCTCCCACC CAATGTGGGA TTCCTGCACG CAGCACCCCC CAGTGGCCGC
    CGGAGGAGGA TCAGCAAAAA TAAGCTGAAA ACTGGGAAAC TGCTCAATCC GCTGGTGCAG
    CAGCTGAAGA ATTCGACTAC CAGTCCAAGC CGCATTTCCC CAGAATGCAA TGGTTCCCCT
    GCTAAGGATC CTGCAAAGTC TCTGGCAGAC TGCACCGCAG GAACCTCGCC AAGCCGTGCC
    GTACAAACGC AGCAGCCTTG CTCTGTGAAA GTCACCATGT CGTCCGATAA TATCTGCTCT
    GGGGTCACCC GGGACGGTGT CGATGTGCAG AGCAATTCAA GCCAAGGCTG GACGGTTTAC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTCTGTC CATCTGGTCC ACTCTGAAAC GCCTGTGTCT AGGCAAAGAT
    CACAGTAGTA GATCTTCGAC AAGCAGCCTC GCAACCGCCC CCGAGGCCTC GGCGCTGCCC
    CCGCTGAACG CACAAATCCA GCCGCAGGCC AACAACAGCA ACAACAACAA AAAGGGGACG
    TTTACGGATG ACCTTCACAA ATTGGTGGAT GAGTGGGCCT GCAAAACCGT CGGCGTGGCA
    CAATTCAAAC CCTCATTAAA CCAATTGAAG CAGTACCAGC AGCGGCAAGA TCTGGAGCCC
    AAGTCTGTGC CCAGCGACCT GTGTGACATG AGCCGTGCCG TGCCAGAGCC CAGGAAGAGA
    TACAGCATTC CCATGGCTGC CCCTCCGGCT GTACCACTGG CTCCCCTCCT CTCTGCCGCT
    GTCCCAGCAA CTGTTCCAGG AGCCGTGCAG CCGGGTACCT TTGTCATGCC CTTTTGTCCG
    TACGCGGCAC CAATGTACGG CATGCCCTGG GCAGGAGCGG CTGAGCTTGG GACTGCAGGA
    AATCAGATCA TGGCCCAAGT AGCCCCCATA GGCAACCAAA TATTCCCTGT GGCACTGCAG
    CACCCAGGTT TGCACCCTGC TGGATCCAAC ATGCGGATTA CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757147.1
    CDS427..6771
    Translation

    Target ORF information:

    RefSeq Version XM_031901287.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901287.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
    GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG
    CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG
    CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT
    TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC
    GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA
    GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG
    AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG
    GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC
    GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG
    GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA
    AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC
    CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC
    GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG
    GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC
    CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC
    ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC
    TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA
    CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG
    TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC
    GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC
    GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC
    TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA
    TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA
    GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA
    CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA
    ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT
    GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC
    CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT
    CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC
    CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT
    CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT
    CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT
    ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG
    ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT
    GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG
    TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA
    CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG
    CTTCCACCGT ACCCTCTCAT TCCAGCAGTT ACTCCTCTCG CAGGACTAGA GACTCTCCCT
    GGCGCCAGTC TCTCTGACTT TCCCGCTGTC TGTGTTACCC CATTACCTGC CCCCTGTCAC
    TACTTTCCCC CAGGCATTGT GGTGGCCAGT GTCCCATTGA CTCCTGCCAT AGCCCCACCC
    CTGCCTTTGC AAGCCATGAA TTCGCAGTTG GTACTGTCCT ATGAGACTAA CATAGTCCCA
    AATGTGCCAG TGGGCACGGT TCCATTGCTA GCAGCAGCCG GTGTCCCAAC GTTACCCACC
    CCACAGGCTG TTTCCCAGCA TCCCCAAGTT CAGCAGTTCC AAACATCCTA CCCACCCCTC
    ATTCAGAGTG ATGTTCCCTC TCAAAATGTC TCCCATCAAG CAACTGTTCC ACCTCCTCAA
    ACCCACCATC CGAGCATCCC TCATCCCTCT GACCAGGCCT CGGCCGGGGC ATGTAACCAG
    GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT
    ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG
    GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA
    ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA
    ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA
    GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT
    GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC
    AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA
    AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG
    ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG
    ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT
    GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG
    GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG
    AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT
    ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA
    GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC
    TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC
    ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA
    TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC
    TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA
    ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA
    GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA
    GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA
    CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA
    AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC
    ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA
    TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT
    ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC
    ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA
    GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC
    TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT
    GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT
    GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC
    AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA
    AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA
    GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC
    CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG
    TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT
    CGCTCTGGAA AGGCCAGTAC CCAAAGCCCC ACCTCTCCCA ACGGCCAAGC CATGAAAATC
    CCTTCAATAA ACGTGACCTC TTTCCATTCG CAGTCGTCCT ATATGAGCAG TGATAACGAC
    TCGGAGTTTG AAGATGCCGA CATGAAGAAG GAACTGCAGA GCCTCCGGGA GAAGCACATG
    AAGGAAATAA CAGAACTGCA ATCGCAGCAG AAGAAGGAGA TTGAGGCCCT TTACCTCCGG
    CTGGGGAAGC CCCTCCCACC CAATGTGGGA TTCCTGCACG CAGCACCCCC CAGTGGCCGC
    CGGAGGAGGA TCAGCAAAAA TAAGCTGAAA ACTGGGAAAC TGCTCAATCC GCTGGTGCAG
    CAGCTGAAGA ATTCGACTAC CAGTCCAAGC CGCATTTCCC CAGAATGCAA TGGTTCCCCT
    GCTAAGGATC CTGCAAAGTC TCTGGCAGAC TGCACCGCAG GAACCTCGCC AAGCCGTGCC
    GTACAAACGC AGCAGCCTTG CTCTGTGAAA GTCACCATGT CGTCCGATAA TATCTGCTCT
    GGGGTCACCC GGGACGGTGT CGATGTGCAG AGCAATTCAA GCCAAGGCTG GACGGTTTAC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTCTGTC CATCTGGTCC ACTCTGAAAC GCCTGTGTCT AGGCAAAGAT
    CACAGTAGTA GATCTTCGAC AAGCAGCCTC GCAACCGCCC CCGAGGCCTC GGCGCTGCCC
    CCGCTGAACG CACAAATCCA GCCGCAGGCC AACAACAGCA ACAACAACAA AAAGGGGACG
    TTTACGGATG ACCTTCACAA ATTGGTGGAT GAGTGGGCCT GCAAAACCGT CGGCGTGGCA
    CAATTCAAAC CCTCATTAAA CCAATTGAAG CAGTACCAGC AGCGGCAAGA TCTGGAGCCC
    AAGTCTGTGC CCAGCGACCT GTGTGACATG AGCCGTGCCG TGCCAGAGCC CAGGAAGAGA
    TACAGCATTC CCATGGCTGC CCCTCCGGCT GTACCACTGG CTCCCCTCCT CTCTGCCGCT
    GTCCCAGCAA CTGTTCCAGG AGCCGTGCAG CCGGGTACCT TTGTCATGCC CTTTTGTCCG
    TACGCGGCAC CAATGTACGG CATGCCCTGG GCAGGAGCGG CTGAGCTTGG GACTGCAGGA
    AATCAGATCA TGGCCCAAGT AGCCCCCATA GGCAACCAAA TATTCCCTGT GGCACTGCAG
    CACCCAGGTT TGCACCCTGC TGGATCCAAC ATGCGGATTA CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57683
    Clone ID Related Accession (Same CDS sequence) XM_031901288.1
    Accession Version XM_031901288.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
    GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG
    CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG
    CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT
    TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC
    GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA
    GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG
    AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG
    GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC
    GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG
    GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA
    AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC
    CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC
    GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG
    GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC
    CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC
    ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC
    TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA
    CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG
    TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC
    GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC
    GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC
    TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA
    TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA
    GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA
    CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA
    ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT
    GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC
    CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT
    CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC
    CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT
    CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT
    CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGACTGGCAC CCTCCCGGGT
    CTTGGGCAGC CGCATTACCA AGACCCCGGT GCCCAGTACC CGATGATGCA AGCGAATGCC
    ATTCCTTCAG TGCAGTTTGC TCAGTTACAG CCAGTGTTAC CTGGACAGCC GCATCCGGTC
    GTACAGCAAA TCCCGGCACC TCAGGGCATC TGCCCCATGC AGTCAGCCAC TCACCCCTTC
    CCACAGTACC AAGCGCCCCT CGCCCAAGTG GCAGTGCCAC AACTGAAGCC TCTCCATATC
    CCTCCTGTGC CCGCACAGCA GCGCCCGGTT CCTGCCACCC AGCTTCCACC GTACCCTCTC
    ATTCCAGCAG TTACTCCTCT CGCAGGACTA GAGACTCTCC CTGGCGCCAG TCTCTCTGAC
    TTTCCCGCTG TCTGTGTTAC CCCATTACCT GCCCCCTGTC ACTACTTTCC CCCAGGCATT
    GTGGTGGCCA GTGTCCCATT GACTCCTGCC ATAGCCCCAC CCCTGCCTTT GCAAGCCATG
    AATTCGCAGT TGGTACTGTC CTATGAGACT AACATAGTCC CAAATGTGCC AGTGGGCACG
    GTTCCATTGC TAGCAGCAGC CGGTGTCCCA ACGTTACCCA CCCCACAGGC TGTTTCCCAG
    CATCCCCAAG TTCAGCAGTT CCAAACATCC TACCCACCCC TCATTCAGAG TGATGTTCCC
    TCTCAAAATG TCTCCCATCA AGCAACTGTT CCACCTCCTC AAACCCACCA TCCGAGCATC
    CCTCATCCCT CTGACCAGGC CTCGGCCGGG GCATGTAACC AGGTTGTTCT GCCCCAATAT
    CCTCTGGATG TGGGAACTGC CATGGGGGTT ATGGTACCTT CTACAACAAT GTCACCACCA
    GTGCAGTTTC CAGCTGAATC TGCCCCTCTG CACCCACCTC AGGAACATGT CACCCCGGTA
    TCCTATATAC ACACAGATGT ACCTATGACC CAACAGAGCA CAACTCTTCA GTCTGAGGGT
    CATCAGCCCG AAACTACACA GCCACCTGTC CATCAGAGTC AAACTCTCTA CCAAGCCCCC
    CGGCCTTCTG TGGAGACCAA TGTAGAGGAG CCGACTCTGC AAGAGAATCC AGTGAGTTCC
    GCACAAAGTT GTGATAACTA TGCAGGCTCA GATGCTGCGT CTGGTAGAGA GATGAGTGAT
    GGTGGAGAAG GGGTTCATGG GAAACAGGAT GGAAAACCAA CCAAAAAGCA TCACCGGAGG
    TCTTCCCGCA CCCGCTCTCG CCAGGAGAAG GCCAATAAAC CAAAGCTGAC TATTCTTAAT
    GTGAGCAATA CAGGTGACAA GATGGTGGAA TGCCAGTTGG AGACTCACAA CCATAAAATG
    GTGACTTTCA AATTTGACTT GGATGGCGAT GCACCAGATG AGATTGCCAC GTACATGGTG
    GAGAATGGCT TTATTCTGCA GATGGAGAAG GAGGTGTTTA TTGAGCAAAT GAAGGATGTT
    ATGGACAAAG CGGAAGACAT GTTGAGCGAA GACACTGAAG GGGAGAGAAG TTCAGACCAA
    GCGGCGAGTC CTCAGAAAGT GGATTCTAAT GGGAGAGAGA CGAGGGAGGA AAACACCCCA
    AATCAAATGA AGACTCCGGT CTATCAGCAA AACGTTCTAC ATACTGGGAA GCGATGGTTT
    ATTATATGTC CAGTGGCCGA AACCACCGGT TCCTCCCTGC CAGAAGTGCC CCAAACTCCC
    TTAAATACCC AGAAGCCGAC TGACGTTAAA GATCAGACCG CCTGCGAGGA TTCAGTATTT
    GCCACAAATG CGGATAAGTT GGACTTTGCG GTGCCGCCGG GCATACTGTG TGAGAGTGCC
    AGTGCCATTC CTGCCTCAAG GACACAATCT TCTTCTACAA TATCTTCCTC CGATCTGCAA
    ACCCAAACGG AACTGTCCTT TTCTGCTCTT CCTGAGTCTC TCTCCTTTCT CCAAGAAGAG
    GAACCGGTCC TGTTCAGCCC GCCGGCAGTT ACGCAGCCTC TAATGGACTC CATAAGCCAT
    GAGGCCGGAG GGAGCCTGGC GCCTGTATTC TCCGCAGCAC AAGAACCCGA GCAGAAACCC
    AGTCCTACAG ATTCAGTAAA TTCAACAGAA CCGCCACGTG CAGCCTTACC CCTCTCCGAC
    CACGAACGAC CGGCCGCTCA GCAAGAACGG ACTCTAGATC CACCCCCAGA GCGGAGTACA
    TTGTATGGAT CCAGTATTGG TTTCCCCGTT GTAAACTCCA TAAAACCAGA TGCCCCCTTG
    CCCATACCCC CTTGCCAGCC ACACCTTGAT TCTGAAATTT CCACCCCACT GAAACCAGTC
    CCAGTCCCCA CTCAGTCTCT GGTTTTACCA GTTGGCGGCA CATCTTGCCA ACAGCCCAGT
    ATTACCGAGT CGGACGGGGA AGGCCCCCCG AGGGTAGAAT TTACCGACGA TACCATTAAA
    AGCTTAGATG AAAAGCTCCG CACTCTCTTG TACCAGGAAT ACACCCCGGC CAACGTTTCT
    GCCGGAACCC CTGACAATTG TTCCGGAGCA GAAAGCGGAG CAGAGACAAC GTTGGGCAAC
    GGAGAAGAGA TTGCTGAACA CAGAAACCTC TCCCAGTCTG TCTCTGCGCC ACTAGAGAAA
    GCAACGGAAC TGGGCTGGAC TGTCGCAGAG AAAAGTTCTT GTGAGCTGTC AAACCAAAAG
    ATTCAGGCTG CACCTCTGAA CCATTCCCAG GGGTGGGATT CTGGCAAAGA AAGTGCAGTT
    GACTCTTCTT CCAAAAAGAG AGAGACCCCT GACAAGTCCT CCAAGACCAT CGGCAGGTTC
    TCGGTTGTGA GTGCAGAAGA TGATGCCCCA GTGACCGCTC CAAATTACAC GAGATTTTCA
    GCCCCGCCCG ATGTCTACCT GGATCAGGCT CCACCACAAC CAGCCAGGGC CTCTGTCCCT
    AGAGCTCACA CCTCTTCCCC TATTAACTCT CTGTTCTGTG GCCATGTTTC CAGTGACTCT
    GGGGATGATA CCCCTCACTC CAAGTCCAAA GAGGCTCAGC CGTCCACCCC AAGTAAGGGG
    GGCACCAATG ACTTCATGAA GAAGGCCGCT GCTTTCTTGC ATCGCTCTGG AAAGGCCAGT
    ACCCAAAGCC CCACCTCTCC CAACGGCCAA GCCATGAAAA TCCCTTCAAT AAACGTGACC
    TCTTTCCATT CGCAGTCGTC CTATATGAGC AGTGATAACG ACTCGGAGTT TGAAGATGCC
    GACATGAAGA AGGAACTGCA GAGCCTCCGG GAGAAGCACA TGAAGGAAAT AACAGAACTG
    CAATCGCAGC AGAAGAAGGA GATTGAGGCC CTTTACCTCC GGCTGGGGAA GCCCCTCCCA
    CCCAATGTGG GATTCCTGCA CGCAGCACCC CCCAGTGGCC GCCGGAGGAG GATCAGCAAA
    AATAAGCTGA AAACTGGGAA ACTGCTCAAT CCGCTGGTGC AGCAGCTGAA GAATTCGACT
    ACCAGTCCAA GCCGCATTTC CCCAGAATGC AATGGTTCCC CTGCTAAGGA TCCTGCAAAG
    TCTCTGGCAG ACTGCACCGC AGGAACCTCG CCAAGCCGTG CCGTACAAAC GCAGCAGCCT
    TGCTCTGTGA AAGTCACCAT GTCGTCCGAT AATATCTGCT CTGGGGTCAC CCGGGACGGT
    GTCGATGTGC AGAGCAATTC AAGCCAAGGC TGGACGGTTT ACCACCAAAC GTCTGAGAGA
    GTGACCTATA AATCTAGTAG CAAACCTCGT ACCAGATTCC TCAGTGGACC TGTGTCTCTG
    TCCATCTGGT CCACTCTGAA ACGCCTGTGT CTAGGCAAAG ATCACAGTAG TAGATCTTCG
    ACAAGCAGCC TCGCAACCGC CCCCGAGGCC TCGGCGCTGC CCCCGCTGAA CGCACAAATC
    CAGCCGCAGG CCAACAACAG CAACAACAAC AAAAAGGGGA CGTTTACGGA TGACCTTCAC
    AAATTGGTGG ATGAGTGGGC CTGCAAAACC GTCGGCGTGG CACAATTCAA ACCCTCATTA
    AACCAATTGA AGCAGTACCA GCAGCGGCAA GATCTGGAGC CCAAGTCTGT GCCCAGCGAC
    CTGTGTGACA TGAGCCGTGC CGTGCCAGAG CCCAGGAAGA GATACAGCAT TCCCATGGCT
    GCCCCTCCGG CTGTACCACT GGCTCCCCTC CTCTCTGCCG CTGTCCCAGC AACTGTTCCA
    GGAGCCGTGC AGCCGGGTAC CTTTGTCATG CCCTTTTGTC CGTACGCGGC ACCAATGTAC
    GGCATGCCCT GGGCAGGAGC GGCTGAGCTT GGGACTGCAG GAAATCAGAT CATGGCCCAA
    GTAGCCCCCA TAGGCAACCA AATATTCCCT GTGGCACTGC AGCACCCAGG TTTGCACCCT
    GCTGGATCCA ACATGCGGAT TACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757148.1
    CDS427..6753
    Translation

    Target ORF information:

    RefSeq Version XM_031901288.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901288.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
    GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG
    CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG
    CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT
    TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC
    GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA
    GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG
    AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG
    GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC
    GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG
    GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA
    AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC
    CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC
    GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG
    GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC
    CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC
    ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC
    TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA
    CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG
    TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC
    GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC
    GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC
    TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA
    TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA
    GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA
    CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA
    ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT
    GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC
    CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT
    CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC
    CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT
    CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT
    CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGACTGGCAC CCTCCCGGGT
    CTTGGGCAGC CGCATTACCA AGACCCCGGT GCCCAGTACC CGATGATGCA AGCGAATGCC
    ATTCCTTCAG TGCAGTTTGC TCAGTTACAG CCAGTGTTAC CTGGACAGCC GCATCCGGTC
    GTACAGCAAA TCCCGGCACC TCAGGGCATC TGCCCCATGC AGTCAGCCAC TCACCCCTTC
    CCACAGTACC AAGCGCCCCT CGCCCAAGTG GCAGTGCCAC AACTGAAGCC TCTCCATATC
    CCTCCTGTGC CCGCACAGCA GCGCCCGGTT CCTGCCACCC AGCTTCCACC GTACCCTCTC
    ATTCCAGCAG TTACTCCTCT CGCAGGACTA GAGACTCTCC CTGGCGCCAG TCTCTCTGAC
    TTTCCCGCTG TCTGTGTTAC CCCATTACCT GCCCCCTGTC ACTACTTTCC CCCAGGCATT
    GTGGTGGCCA GTGTCCCATT GACTCCTGCC ATAGCCCCAC CCCTGCCTTT GCAAGCCATG
    AATTCGCAGT TGGTACTGTC CTATGAGACT AACATAGTCC CAAATGTGCC AGTGGGCACG
    GTTCCATTGC TAGCAGCAGC CGGTGTCCCA ACGTTACCCA CCCCACAGGC TGTTTCCCAG
    CATCCCCAAG TTCAGCAGTT CCAAACATCC TACCCACCCC TCATTCAGAG TGATGTTCCC
    TCTCAAAATG TCTCCCATCA AGCAACTGTT CCACCTCCTC AAACCCACCA TCCGAGCATC
    CCTCATCCCT CTGACCAGGC CTCGGCCGGG GCATGTAACC AGGTTGTTCT GCCCCAATAT
    CCTCTGGATG TGGGAACTGC CATGGGGGTT ATGGTACCTT CTACAACAAT GTCACCACCA
    GTGCAGTTTC CAGCTGAATC TGCCCCTCTG CACCCACCTC AGGAACATGT CACCCCGGTA
    TCCTATATAC ACACAGATGT ACCTATGACC CAACAGAGCA CAACTCTTCA GTCTGAGGGT
    CATCAGCCCG AAACTACACA GCCACCTGTC CATCAGAGTC AAACTCTCTA CCAAGCCCCC
    CGGCCTTCTG TGGAGACCAA TGTAGAGGAG CCGACTCTGC AAGAGAATCC AGTGAGTTCC
    GCACAAAGTT GTGATAACTA TGCAGGCTCA GATGCTGCGT CTGGTAGAGA GATGAGTGAT
    GGTGGAGAAG GGGTTCATGG GAAACAGGAT GGAAAACCAA CCAAAAAGCA TCACCGGAGG
    TCTTCCCGCA CCCGCTCTCG CCAGGAGAAG GCCAATAAAC CAAAGCTGAC TATTCTTAAT
    GTGAGCAATA CAGGTGACAA GATGGTGGAA TGCCAGTTGG AGACTCACAA CCATAAAATG
    GTGACTTTCA AATTTGACTT GGATGGCGAT GCACCAGATG AGATTGCCAC GTACATGGTG
    GAGAATGGCT TTATTCTGCA GATGGAGAAG GAGGTGTTTA TTGAGCAAAT GAAGGATGTT
    ATGGACAAAG CGGAAGACAT GTTGAGCGAA GACACTGAAG GGGAGAGAAG TTCAGACCAA
    GCGGCGAGTC CTCAGAAAGT GGATTCTAAT GGGAGAGAGA CGAGGGAGGA AAACACCCCA
    AATCAAATGA AGACTCCGGT CTATCAGCAA AACGTTCTAC ATACTGGGAA GCGATGGTTT
    ATTATATGTC CAGTGGCCGA AACCACCGGT TCCTCCCTGC CAGAAGTGCC CCAAACTCCC
    TTAAATACCC AGAAGCCGAC TGACGTTAAA GATCAGACCG CCTGCGAGGA TTCAGTATTT
    GCCACAAATG CGGATAAGTT GGACTTTGCG GTGCCGCCGG GCATACTGTG TGAGAGTGCC
    AGTGCCATTC CTGCCTCAAG GACACAATCT TCTTCTACAA TATCTTCCTC CGATCTGCAA
    ACCCAAACGG AACTGTCCTT TTCTGCTCTT CCTGAGTCTC TCTCCTTTCT CCAAGAAGAG
    GAACCGGTCC TGTTCAGCCC GCCGGCAGTT ACGCAGCCTC TAATGGACTC CATAAGCCAT
    GAGGCCGGAG GGAGCCTGGC GCCTGTATTC TCCGCAGCAC AAGAACCCGA GCAGAAACCC
    AGTCCTACAG ATTCAGTAAA TTCAACAGAA CCGCCACGTG CAGCCTTACC CCTCTCCGAC
    CACGAACGAC CGGCCGCTCA GCAAGAACGG ACTCTAGATC CACCCCCAGA GCGGAGTACA
    TTGTATGGAT CCAGTATTGG TTTCCCCGTT GTAAACTCCA TAAAACCAGA TGCCCCCTTG
    CCCATACCCC CTTGCCAGCC ACACCTTGAT TCTGAAATTT CCACCCCACT GAAACCAGTC
    CCAGTCCCCA CTCAGTCTCT GGTTTTACCA GTTGGCGGCA CATCTTGCCA ACAGCCCAGT
    ATTACCGAGT CGGACGGGGA AGGCCCCCCG AGGGTAGAAT TTACCGACGA TACCATTAAA
    AGCTTAGATG AAAAGCTCCG CACTCTCTTG TACCAGGAAT ACACCCCGGC CAACGTTTCT
    GCCGGAACCC CTGACAATTG TTCCGGAGCA GAAAGCGGAG CAGAGACAAC GTTGGGCAAC
    GGAGAAGAGA TTGCTGAACA CAGAAACCTC TCCCAGTCTG TCTCTGCGCC ACTAGAGAAA
    GCAACGGAAC TGGGCTGGAC TGTCGCAGAG AAAAGTTCTT GTGAGCTGTC AAACCAAAAG
    ATTCAGGCTG CACCTCTGAA CCATTCCCAG GGGTGGGATT CTGGCAAAGA AAGTGCAGTT
    GACTCTTCTT CCAAAAAGAG AGAGACCCCT GACAAGTCCT CCAAGACCAT CGGCAGGTTC
    TCGGTTGTGA GTGCAGAAGA TGATGCCCCA GTGACCGCTC CAAATTACAC GAGATTTTCA
    GCCCCGCCCG ATGTCTACCT GGATCAGGCT CCACCACAAC CAGCCAGGGC CTCTGTCCCT
    AGAGCTCACA CCTCTTCCCC TATTAACTCT CTGTTCTGTG GCCATGTTTC CAGTGACTCT
    GGGGATGATA CCCCTCACTC CAAGTCCAAA GAGGCTCAGC CGTCCACCCC AAGTAAGGGG
    GGCACCAATG ACTTCATGAA GAAGGCCGCT GCTTTCTTGC ATCGCTCTGG AAAGGCCAGT
    ACCCAAAGCC CCACCTCTCC CAACGGCCAA GCCATGAAAA TCCCTTCAAT AAACGTGACC
    TCTTTCCATT CGCAGTCGTC CTATATGAGC AGTGATAACG ACTCGGAGTT TGAAGATGCC
    GACATGAAGA AGGAACTGCA GAGCCTCCGG GAGAAGCACA TGAAGGAAAT AACAGAACTG
    CAATCGCAGC AGAAGAAGGA GATTGAGGCC CTTTACCTCC GGCTGGGGAA GCCCCTCCCA
    CCCAATGTGG GATTCCTGCA CGCAGCACCC CCCAGTGGCC GCCGGAGGAG GATCAGCAAA
    AATAAGCTGA AAACTGGGAA ACTGCTCAAT CCGCTGGTGC AGCAGCTGAA GAATTCGACT
    ACCAGTCCAA GCCGCATTTC CCCAGAATGC AATGGTTCCC CTGCTAAGGA TCCTGCAAAG
    TCTCTGGCAG ACTGCACCGC AGGAACCTCG CCAAGCCGTG CCGTACAAAC GCAGCAGCCT
    TGCTCTGTGA AAGTCACCAT GTCGTCCGAT AATATCTGCT CTGGGGTCAC CCGGGACGGT
    GTCGATGTGC AGAGCAATTC AAGCCAAGGC TGGACGGTTT ACCACCAAAC GTCTGAGAGA
    GTGACCTATA AATCTAGTAG CAAACCTCGT ACCAGATTCC TCAGTGGACC TGTGTCTCTG
    TCCATCTGGT CCACTCTGAA ACGCCTGTGT CTAGGCAAAG ATCACAGTAG TAGATCTTCG
    ACAAGCAGCC TCGCAACCGC CCCCGAGGCC TCGGCGCTGC CCCCGCTGAA CGCACAAATC
    CAGCCGCAGG CCAACAACAG CAACAACAAC AAAAAGGGGA CGTTTACGGA TGACCTTCAC
    AAATTGGTGG ATGAGTGGGC CTGCAAAACC GTCGGCGTGG CACAATTCAA ACCCTCATTA
    AACCAATTGA AGCAGTACCA GCAGCGGCAA GATCTGGAGC CCAAGTCTGT GCCCAGCGAC
    CTGTGTGACA TGAGCCGTGC CGTGCCAGAG CCCAGGAAGA GATACAGCAT TCCCATGGCT
    GCCCCTCCGG CTGTACCACT GGCTCCCCTC CTCTCTGCCG CTGTCCCAGC AACTGTTCCA
    GGAGCCGTGC AGCCGGGTAC CTTTGTCATG CCCTTTTGTC CGTACGCGGC ACCAATGTAC
    GGCATGCCCT GGGCAGGAGC GGCTGAGCTT GGGACTGCAG GAAATCAGAT CATGGCCCAA
    GTAGCCCCCA TAGGCAACCA AATATTCCCT GTGGCACTGC AGCACCCAGG TTTGCACCCT
    GCTGGATCCA ACATGCGGAT TACGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57684
    Clone ID Related Accession (Same CDS sequence) XM_031901289.1
    Accession Version XM_031901289.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
    GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG
    CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG
    CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT
    TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC
    GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA
    GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG
    AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG
    GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC
    GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG
    GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA
    AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC
    CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC
    GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG
    GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC
    CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC
    ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC
    TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA
    CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG
    TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC
    GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC
    GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC
    TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA
    TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA
    GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA
    CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA
    ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT
    GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC
    CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT
    CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC
    CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT
    CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT
    CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT
    ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG
    ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT
    GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG
    TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA
    CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG
    CTTCCACCGT ACCCTCTCAT TCCAGCAGTT ACTCCTCTCG CAGGACTAGA GACTCTCCCT
    GGCGCCAGTC TCTCTGACTT TCCCGCTGTC TGTGTTACCC CATTACCTGC CCCCTGTCAC
    TACTTTCCCC CAGGCATTGT GGTGGCCAGT GTCCCATTGA CTCCTGCCAT AGCCCCACCC
    CTGCCTTTGC AAGCCATGAA TTCGCAGTTG GTACTGTCCT ATGAGACTAA CATAGTCCCA
    AATGTGCCAG TGGGCACGGT TCCATTGCTA GCAGCAGCCG GTGTCCCAAC GTTACCCACC
    CCACAGGCTG TTTCCCAGCA TCCCCAAGTT CAGCAGTTCC AAACATCCTA CCCACCCCTC
    ATTCAGAGTG ATGTTCCCTC TCAAAATGTC TCCCATCAAG CAACTGTTCC ACCTCCTCAA
    ACCCACCATC CGAGCATCCC TCATCCCTCT GACCAGGCCT CGGCCGGGGC ATGTAACCAG
    GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT
    ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG
    GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA
    ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA
    ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA
    GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT
    GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC
    AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA
    AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG
    ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG
    ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT
    GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG
    GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG
    AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT
    ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA
    GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC
    TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC
    ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA
    TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC
    TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA
    ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA
    GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA
    GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA
    CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA
    AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC
    ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA
    TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT
    ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC
    ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA
    GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC
    TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT
    GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT
    GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC
    AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA
    AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA
    GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC
    CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG
    TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT
    CGCTCTGGAA AGTCGTCCTA TATGAGCAGT GATAACGACT CGGAGTTTGA AGATGCCGAC
    ATGAAGAAGG AACTGCAGAG CCTCCGGGAG AAGCACATGA AGGAAATAAC AGAACTGCAA
    TCGCAGCAGA AGAAGGAGAT TGAGGCCCTT TACCTCCGGC TGGGGAAGCC CCTCCCACCC
    AATGTGGGAT TCCTGCACGC AGCACCCCCC AGTGGCCGCC GGAGGAGGAT CAGCAAAAAT
    AAGCTGAAAA CTGGGAAACT GCTCAATCCG CTGGTGCAGC AGCTGAAGAA TTCGACTACC
    AGTCCAAGCC GCATTTCCCC AGAATGCAAT GGTTCCCCTG CTAAGGATCC TGCAAAGTCT
    CTGGCAGACT GCACCGCAGG AACCTCGCCA AGCCGTGCCG TACAAACGCA GCAGCCTTGC
    TCTGTGAAAG TCACCATGTC GTCCGATAAT ATCTGCTCTG GGGTCACCCG GGACGGTGTC
    GATGTGCAGA GCAATTCAAG CCAAGGCTGG ACGGTTTACC ACCAAACGTC TGAGAGAGTG
    ACCTATAAAT CTAGTAGCAA ACCTCGTACC AGATTCCTCA GTGGACCTGT GTCTCTGTCC
    ATCTGGTCCA CTCTGAAACG CCTGTGTCTA GGCAAAGATC ACAGTAGTAG ATCTTCGACA
    AGCAGCCTCG CAACCGCCCC CGAGGCCTCG GCGCTGCCCC CGCTGAACGC ACAAATCCAG
    CCGCAGGCCA ACAACAGCAA CAACAACAAA AAGGGGACGT TTACGGATGA CCTTCACAAA
    TTGGTGGATG AGTGGGCCTG CAAAACCGTC GGCGTGGCAC AATTCAAACC CTCATTAAAC
    CAATTGAAGC AGTACCAGCA GCGGCAAGAT CTGGAGCCCA AGTCTGTGCC CAGCGACCTG
    TGTGACATGA GCCGTGCCGT GCCAGAGCCC AGGAAGAGAT ACAGCATTCC CATGGCTGCC
    CCTCCGGCTG TACCACTGGC TCCCCTCCTC TCTGCCGCTG TCCCAGCAAC TGTTCCAGGA
    GCCGTGCAGC CGGGTACCTT TGTCATGCCC TTTTGTCCGT ACGCGGCACC AATGTACGGC
    ATGCCCTGGG CAGGAGCGGC TGAGCTTGGG ACTGCAGGAA ATCAGATCAT GGCCCAAGTA
    GCCCCCATAG GCAACCAAAT ATTCCCTGTG GCACTGCAGC ACCCAGGTTT GCACCCTGCT
    GGATCCAACA TGCGGATTAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757149.1
    CDS427..6690
    Translation

    Target ORF information:

    RefSeq Version XM_031901289.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901289.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
    GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG
    CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG
    CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT
    TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC
    GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA
    GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG
    AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG
    GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC
    GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG
    GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA
    AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC
    CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC
    GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG
    GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC
    CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC
    ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC
    TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA
    CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG
    TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC
    GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC
    GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC
    TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA
    TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA
    GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA
    CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA
    ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT
    GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC
    CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT
    CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC
    CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT
    CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT
    CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT
    ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG
    ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT
    GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG
    TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA
    CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG
    CTTCCACCGT ACCCTCTCAT TCCAGCAGTT ACTCCTCTCG CAGGACTAGA GACTCTCCCT
    GGCGCCAGTC TCTCTGACTT TCCCGCTGTC TGTGTTACCC CATTACCTGC CCCCTGTCAC
    TACTTTCCCC CAGGCATTGT GGTGGCCAGT GTCCCATTGA CTCCTGCCAT AGCCCCACCC
    CTGCCTTTGC AAGCCATGAA TTCGCAGTTG GTACTGTCCT ATGAGACTAA CATAGTCCCA
    AATGTGCCAG TGGGCACGGT TCCATTGCTA GCAGCAGCCG GTGTCCCAAC GTTACCCACC
    CCACAGGCTG TTTCCCAGCA TCCCCAAGTT CAGCAGTTCC AAACATCCTA CCCACCCCTC
    ATTCAGAGTG ATGTTCCCTC TCAAAATGTC TCCCATCAAG CAACTGTTCC ACCTCCTCAA
    ACCCACCATC CGAGCATCCC TCATCCCTCT GACCAGGCCT CGGCCGGGGC ATGTAACCAG
    GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT
    ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG
    GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA
    ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA
    ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA
    GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT
    GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC
    AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA
    AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG
    ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG
    ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT
    GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG
    GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG
    AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT
    ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA
    GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC
    TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC
    ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA
    TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC
    TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA
    ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA
    GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA
    GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA
    CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA
    AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC
    ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA
    TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT
    ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC
    ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA
    GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC
    TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT
    GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT
    GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC
    AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA
    AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA
    GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC
    CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG
    TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT
    CGCTCTGGAA AGTCGTCCTA TATGAGCAGT GATAACGACT CGGAGTTTGA AGATGCCGAC
    ATGAAGAAGG AACTGCAGAG CCTCCGGGAG AAGCACATGA AGGAAATAAC AGAACTGCAA
    TCGCAGCAGA AGAAGGAGAT TGAGGCCCTT TACCTCCGGC TGGGGAAGCC CCTCCCACCC
    AATGTGGGAT TCCTGCACGC AGCACCCCCC AGTGGCCGCC GGAGGAGGAT CAGCAAAAAT
    AAGCTGAAAA CTGGGAAACT GCTCAATCCG CTGGTGCAGC AGCTGAAGAA TTCGACTACC
    AGTCCAAGCC GCATTTCCCC AGAATGCAAT GGTTCCCCTG CTAAGGATCC TGCAAAGTCT
    CTGGCAGACT GCACCGCAGG AACCTCGCCA AGCCGTGCCG TACAAACGCA GCAGCCTTGC
    TCTGTGAAAG TCACCATGTC GTCCGATAAT ATCTGCTCTG GGGTCACCCG GGACGGTGTC
    GATGTGCAGA GCAATTCAAG CCAAGGCTGG ACGGTTTACC ACCAAACGTC TGAGAGAGTG
    ACCTATAAAT CTAGTAGCAA ACCTCGTACC AGATTCCTCA GTGGACCTGT GTCTCTGTCC
    ATCTGGTCCA CTCTGAAACG CCTGTGTCTA GGCAAAGATC ACAGTAGTAG ATCTTCGACA
    AGCAGCCTCG CAACCGCCCC CGAGGCCTCG GCGCTGCCCC CGCTGAACGC ACAAATCCAG
    CCGCAGGCCA ACAACAGCAA CAACAACAAA AAGGGGACGT TTACGGATGA CCTTCACAAA
    TTGGTGGATG AGTGGGCCTG CAAAACCGTC GGCGTGGCAC AATTCAAACC CTCATTAAAC
    CAATTGAAGC AGTACCAGCA GCGGCAAGAT CTGGAGCCCA AGTCTGTGCC CAGCGACCTG
    TGTGACATGA GCCGTGCCGT GCCAGAGCCC AGGAAGAGAT ACAGCATTCC CATGGCTGCC
    CCTCCGGCTG TACCACTGGC TCCCCTCCTC TCTGCCGCTG TCCCAGCAAC TGTTCCAGGA
    GCCGTGCAGC CGGGTACCTT TGTCATGCCC TTTTGTCCGT ACGCGGCACC AATGTACGGC
    ATGCCCTGGG CAGGAGCGGC TGAGCTTGGG ACTGCAGGAA ATCAGATCAT GGCCCAAGTA
    GCCCCCATAG GCAACCAAAT ATTCCCTGTG GCACTGCAGC ACCCAGGTTT GCACCCTGCT
    GGATCCAACA TGCGGATTAC GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa57685
    Clone ID Related Accession (Same CDS sequence) XM_031901290.1
    Accession Version XM_031901290.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product serine/threonine-protein kinase WNK2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
    GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG
    CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG
    CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT
    TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC
    GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA
    GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG
    AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG
    GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC
    GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG
    GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA
    AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC
    CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC
    GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG
    GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC
    CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC
    ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC
    TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA
    CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG
    TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC
    GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC
    GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC
    TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA
    TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA
    GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA
    CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA
    ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT
    GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC
    CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT
    CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC
    CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT
    CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT
    CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT
    ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG
    ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT
    GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG
    TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA
    CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG
    CTTCCACCGT ACCCTCTCAT TCCAGCAGTT ACTCCTCTCG CAGGACTAGA GACTCTCCCT
    GGCGCCAGTC TCTCTGACTT TCCCGCTGTC TGTGTTACCC CATTACCTGC CCCCTGTCAC
    TACTTTCCCC CAGGCATTGT GGTGGCCAGT GTCCCATTGA CTCCTGCCAT AGCCCCACCC
    CTGCCTTTGC AAGCCATGAA TTCGCAGTTG GTACTGTCCT ATGAGACTAA CATAGTCCCA
    AATGTGCCAG TGGGCACGGT TCCATTGCTA GCAGCAGCCG GTGTCCCAAC GTTACCCACC
    CCACAGGCTG TTTCCCAGCA TCCCCAAGTT CAGCAGTTCC AAACATCCTA CCCACCCCTC
    ATTCAGAGTG ATGTTCCCTC TCAAAATGTC TCCCATCAAG CAACTGTTCC ACCTCCTCAA
    ACCCACCATC CGAGCATCCC TCATCCCTCT GACCAGGCCT CGGCCGGGGC ATGTAACCAG
    GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT
    ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG
    GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA
    ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA
    ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA
    GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT
    GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC
    AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA
    AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG
    ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG
    ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT
    GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG
    GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG
    AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT
    ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA
    GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC
    TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC
    ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA
    TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC
    TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA
    ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA
    GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA
    GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA
    CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA
    AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC
    ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA
    TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT
    ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC
    ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA
    GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC
    TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT
    GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT
    GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC
    AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA
    AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA
    GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC
    CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG
    TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT
    CGCTCTGGAA AGGCCAGTAC CCAAAGCCCC ACCTCTCCCA ACGGCCAAGC CATGAAAATC
    CCTTCAATAA ACGTGACCTC TTTCCATTCG CAGTCGTCCT ATATGAGCAG TGATAACGAC
    TCGGAGTTTG AAGATGCCGA CATGAAGAAG GAACTGCAGA GCCTCCGGGA GAAGCACATG
    AAGGAAATAA CAGAACTGCA ATCGCAGCAG AAGAAGGAGA TTGAGGCCCT TTACCTCCGG
    CTGGGGAAGC CCCTCCCACC CAATGTGGGA TTCCTGCACG CAGCACCCCC CAGTGGCCGC
    CGGAGGAGGA TCAGCAAAAA TAAGCTGAAA ACTGGGAAAC TGCTCAATCC GCTGGTGCAG
    CAGCTGAAGA ATTCGACTAC CAGTCCAAGC CGCATTTCCC CAGAATGCAA TGGTTCCCCT
    GCTAAGGATC CTGCAAAGTC TCTGGCAGAC TGCACCGCAG GAACCTCGCC AAGCCGTGCC
    GTACAAACGC AGCAGCCTTG CTCTGTGAAA GTCACCATGT CGTCCGATAA TATCTGCTCT
    GGGGTCACCC GGGACGGTGT CGATGTGCAG AGCAATTCAA GCCAAGGATC TTCGACAAGC
    AGCCTCGCAA CCGCCCCCGA GGCCTCGGCG CTGCCCCCGC TGAACGCACA AATCCAGCCG
    CAGGCCAACA ACAGCAACAA CAACAAAAAG GGGACGTTTA CGGATGACCT TCACAAATTG
    GTGGATGAGT GGGCCTGCAA AACCGTCGGC GTGGCACAAT TCAAACCCTC ATTAAACCAA
    TTGAAGCAGT ACCAGCAGCG GCAAGATCTG GAGCCCAAGT CTGTGCCCAG CGACCTGTGT
    GACATGAGCC GTGCCGTGCC AGAGCCCAGG AAGAGATACA GCATTCCCAT GGCTGCCCCT
    CCGGCTGTAC CACTGGCTCC CCTCCTCTCT GCCGCTGTCC CAGCAACTGT TCCAGGAGCC
    GTGCAGCCGG GTACCTTTGT CATGCCCTTT TGTCCGTACG CGGCACCAAT GTACGGCATG
    CCCTGGGCAG GAGCGGCTGA GCTTGGGACT GCAGGAAATC AGATCATGGC CCAAGTAGCC
    CCCATAGGCA ACCAAATATT CCCTGTGGCA CTGCAGCACC CAGGTTTGCA CCCTGCTGGA
    TCCAACATGC GGATTACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757150.1
    CDS426..6626
    Translation

    Target ORF information:

    RefSeq Version XM_031901290.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis WNK lysine deficient protein kinase 2 (wnk2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901290.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGAGGCCG GGGTAGGTGC CCCCTCATGT ATTGGCAGCC TGGGCACCGT GGAACCCAAT 
    GGTCTGGCCC AGCTTCCCAT TAATATTCAG CCCATTGCTA AAGGTGTGGA TACTGCAATG
    CTGAGCTCGG GCGCCGGTGC CCAGGCTGGA CCAGCTGCCA AGGTCTCAAA GACGGTCAAG
    CGGAGGTTCT TGAGAAGGAC AGTGGTGGAC TCTGACCAAG AGGAGCCCTC AGTGTTGGAT
    TTATCATTAT CCGGGCACCA CAACAAGGTC ATCCCCATCT CCCGGGCACG GAGAGCCATC
    GCCAAGAGGT CTCAGGGGGT GAGCGACCAG GGAGGGGGCA CCGAGGTCTT GGAATCCAAA
    GGGTCTTCCT CTAAAGGGGG CAGCGCAGAC GGGGAACCGG ATCAACCCGA GGGGCTCGAG
    AACAAGGAGA AAGCGGAAAC CTCCCATGAT GTACCAAAGT GCAGAAAGGA GGCCCCAGCG
    GAGGAGGAAG AGGAGAATGA AGAGGAGGCC GACATGAAGG CCGTCTCTAC CTCCCTTGAC
    GGAAGGTTTC TAAAGTTTGA TATTGAAATA GGACGCGGCT CCTTTAAGAC GGTCTATAAG
    GGCTTGGACA CCGAGACCTG GGTGGAAGTG GCCTGGTGTG AGTTGCAGGA CCGTAAATTA
    AGCAAATTAG AGCGCCAGCG GTTTAAGGAG GAAGCGGAGA TGCTGAAGGG GCTGCAGCAC
    CCGAATATCG TCCGATTCTA TGACTTCTGG GAGTCGTGTC TGAAAGGGAA GAAGTACATC
    GTTCTTGTTA CGGAGCTCAT GACATCGGGG ACCCTAAAAA CATACCTGAA GAGATTTAAG
    GTGATGAAGC CCAAGGTCTT GCGCAGTTGG TGCCGGCAGA TCCTCAAGGG GTTAATGTTC
    CTCCACACGC GCACCCCTCC CATCATCCAC CGGGACCTCA AATGCGACAA CATCTTCATC
    ACTGGACCCA CCGGCTCGGT GAAAATCGGC GACCTGGGCC TGGCCACCCT GAAGCGCGCC
    TCCTTCGCCA AGAGCGTCAT AGGCACCCCC GAGTTCATGG CCCCCGAGAT GTACGAGGAA
    CATTACGACG AGTCGGTGGA CGTTTACGCC TTCGGGATGT GCATGTTGGA AATGGCCACG
    TCGGAATATC CGTACTCGGA ATGCCAGAAC GCGGCCCAGA TCTACCGGAA AGTGACCAGC
    GGGGTGAAGC CCGCGAGCTT TGATAAAGTT GCAGACCCGG AGATCAAGGA AATCATCGGC
    GAGTGCATCT GCAAGAATAA AGAGGAGAGA TATGGAATTA AAGACTTACT AAGCCACGCC
    TTCTTCGCGG AAGACACGGG GGTGCGGGTG GAGCTGGCAG AGGAAGACAA CGGTAGAAAA
    TCTTCCATTG CCTTAAGGCT GTGGGTGGAG GATCCCAAGA AGCTGAAAGT CAAACCCAAA
    GATAACGGCG CCATCGAGTT CACTTTCGAT CTGGAGAAGG AAACTCCTGA GGATGTGGCA
    CAAGAGATGG TGGAATCCGG GTTCTTCCAT GAGAGCGACG TGAAGATTGT GGGCAAATCA
    ATAAGAGACC GAGTTGCGCT GATCCAGTGG CGGAGAGAGA GGATCTGTCC GGGGGAGAAT
    GAGGCCTTGG AGCAGCCGCC AGTAGTGCAG CCGCCACTCG CCCCCTTGGC ACACTCCTCC
    CACATTGCCC CCCACATCCC CCCCGAGCCA GAGGAACTGG ACACCGACCA GCACTTGTAT
    CAGCCCAATC TGCCAACCAG CATCACATCT GTTGCCTCCG ACGGCACGTT TGACAGCGGC
    CAGGGATCCA CCGTGTATTC AGATTCTCAG AGCAGCCAGC AAAGCGTCAT CTATTCCTCT
    CTGCCCGACT CACTGCCTCC GGCCATCCAG AGGGTCTATA GCCCCCCGAT CCTGGAAGGT
    CAGACCGTGC CACACTTTGG GCAGTACCAG CCGCCTCCCA CGCCTGGGAT ACAAGTTGCT
    ACTGGCACCC TCCCGGGTCT TGGGCAGCCG CATTACCAAG ACCCCGGTGC CCAGTACCCG
    ATGATGCAAG CGAATGCCAT TCCTTCAGTG CAGTTTGCTC AGTTACAGCC AGTGTTACCT
    GGACAGCCGC ATCCGGTCGT ACAGCAAATC CCGGCACCTC AGGGCATCTG CCCCATGCAG
    TCAGCCACTC ACCCCTTCCC ACAGTACCAA GCGCCCCTCG CCCAAGTGGC AGTGCCACAA
    CTGAAGCCTC TCCATATCCC TCCTGTGCCC GCACAGCAGC GCCCGGTTCC TGCCACCCAG
    CTTCCACCGT ACCCTCTCAT TCCAGCAGTT ACTCCTCTCG CAGGACTAGA GACTCTCCCT
    GGCGCCAGTC TCTCTGACTT TCCCGCTGTC TGTGTTACCC CATTACCTGC CCCCTGTCAC
    TACTTTCCCC CAGGCATTGT GGTGGCCAGT GTCCCATTGA CTCCTGCCAT AGCCCCACCC
    CTGCCTTTGC AAGCCATGAA TTCGCAGTTG GTACTGTCCT ATGAGACTAA CATAGTCCCA
    AATGTGCCAG TGGGCACGGT TCCATTGCTA GCAGCAGCCG GTGTCCCAAC GTTACCCACC
    CCACAGGCTG TTTCCCAGCA TCCCCAAGTT CAGCAGTTCC AAACATCCTA CCCACCCCTC
    ATTCAGAGTG ATGTTCCCTC TCAAAATGTC TCCCATCAAG CAACTGTTCC ACCTCCTCAA
    ACCCACCATC CGAGCATCCC TCATCCCTCT GACCAGGCCT CGGCCGGGGC ATGTAACCAG
    GTTGTTCTGC CCCAATATCC TCTGGATGTG GGAACTGCCA TGGGGGTTAT GGTACCTTCT
    ACAACAATGT CACCACCAGT GCAGTTTCCA GCTGAATCTG CCCCTCTGCA CCCACCTCAG
    GAACATGTCA CCCCGGTATC CTATATACAC ACAGATGTAC CTATGACCCA ACAGAGCACA
    ACTCTTCAGT CTGAGGGTCA TCAGCCCGAA ACTACACAGC CACCTGTCCA TCAGAGTCAA
    ACTCTCTACC AAGCCCCCCG GCCTTCTGTG GAGACCAATG TAGAGGAGCC GACTCTGCAA
    GAGAATCCAG TGAGTTCCGC ACAAAGTTGT GATAACTATG CAGGCTCAGA TGCTGCGTCT
    GGTAGAGAGA TGAGTGATGG TGGAGAAGGG GTTCATGGGA AACAGGATGG AAAACCAACC
    AAAAAGCATC ACCGGAGGTC TTCCCGCACC CGCTCTCGCC AGGAGAAGGC CAATAAACCA
    AAGCTGACTA TTCTTAATGT GAGCAATACA GGTGACAAGA TGGTGGAATG CCAGTTGGAG
    ACTCACAACC ATAAAATGGT GACTTTCAAA TTTGACTTGG ATGGCGATGC ACCAGATGAG
    ATTGCCACGT ACATGGTGGA GAATGGCTTT ATTCTGCAGA TGGAGAAGGA GGTGTTTATT
    GAGCAAATGA AGGATGTTAT GGACAAAGCG GAAGACATGT TGAGCGAAGA CACTGAAGGG
    GAGAGAAGTT CAGACCAAGC GGCGAGTCCT CAGAAAGTGG ATTCTAATGG GAGAGAGACG
    AGGGAGGAAA ACACCCCAAA TCAAATGAAG ACTCCGGTCT ATCAGCAAAA CGTTCTACAT
    ACTGGGAAGC GATGGTTTAT TATATGTCCA GTGGCCGAAA CCACCGGTTC CTCCCTGCCA
    GAAGTGCCCC AAACTCCCTT AAATACCCAG AAGCCGACTG ACGTTAAAGA TCAGACCGCC
    TGCGAGGATT CAGTATTTGC CACAAATGCG GATAAGTTGG ACTTTGCGGT GCCGCCGGGC
    ATACTGTGTG AGAGTGCCAG TGCCATTCCT GCCTCAAGGA CACAATCTTC TTCTACAATA
    TCTTCCTCCG ATCTGCAAAC CCAAACGGAA CTGTCCTTTT CTGCTCTTCC TGAGTCTCTC
    TCCTTTCTCC AAGAAGAGGA ACCGGTCCTG TTCAGCCCGC CGGCAGTTAC GCAGCCTCTA
    ATGGACTCCA TAAGCCATGA GGCCGGAGGG AGCCTGGCGC CTGTATTCTC CGCAGCACAA
    GAACCCGAGC AGAAACCCAG TCCTACAGAT TCAGTAAATT CAACAGAACC GCCACGTGCA
    GCCTTACCCC TCTCCGACCA CGAACGACCG GCCGCTCAGC AAGAACGGAC TCTAGATCCA
    CCCCCAGAGC GGAGTACATT GTATGGATCC AGTATTGGTT TCCCCGTTGT AAACTCCATA
    AAACCAGATG CCCCCTTGCC CATACCCCCT TGCCAGCCAC ACCTTGATTC TGAAATTTCC
    ACCCCACTGA AACCAGTCCC AGTCCCCACT CAGTCTCTGG TTTTACCAGT TGGCGGCACA
    TCTTGCCAAC AGCCCAGTAT TACCGAGTCG GACGGGGAAG GCCCCCCGAG GGTAGAATTT
    ACCGACGATA CCATTAAAAG CTTAGATGAA AAGCTCCGCA CTCTCTTGTA CCAGGAATAC
    ACCCCGGCCA ACGTTTCTGC CGGAACCCCT GACAATTGTT CCGGAGCAGA AAGCGGAGCA
    GAGACAACGT TGGGCAACGG AGAAGAGATT GCTGAACACA GAAACCTCTC CCAGTCTGTC
    TCTGCGCCAC TAGAGAAAGC AACGGAACTG GGCTGGACTG TCGCAGAGAA AAGTTCTTGT
    GAGCTGTCAA ACCAAAAGAT TCAGGCTGCA CCTCTGAACC ATTCCCAGGG GTGGGATTCT
    GGCAAAGAAA GTGCAGTTGA CTCTTCTTCC AAAAAGAGAG AGACCCCTGA CAAGTCCTCC
    AAGACCATCG GCAGGTTCTC GGTTGTGAGT GCAGAAGATG ATGCCCCAGT GACCGCTCCA
    AATTACACGA GATTTTCAGC CCCGCCCGAT GTCTACCTGG ATCAGGCTCC ACCACAACCA
    GCCAGGGCCT CTGTCCCTAG AGCTCACACC TCTTCCCCTA TTAACTCTCT GTTCTGTGGC
    CATGTTTCCA GTGACTCTGG GGATGATACC CCTCACTCCA AGTCCAAAGA GGCTCAGCCG
    TCCACCCCAA GTAAGGGGGG CACCAATGAC TTCATGAAGA AGGCCGCTGC TTTCTTGCAT
    CGCTCTGGAA AGGCCAGTAC CCAAAGCCCC ACCTCTCCCA ACGGCCAAGC CATGAAAATC
    CCTTCAATAA ACGTGACCTC TTTCCATTCG CAGTCGTCCT ATATGAGCAG TGATAACGAC
    TCGGAGTTTG AAGATGCCGA CATGAAGAAG GAACTGCAGA GCCTCCGGGA GAAGCACATG
    AAGGAAATAA CAGAACTGCA ATCGCAGCAG AAGAAGGAGA TTGAGGCCCT TTACCTCCGG
    CTGGGGAAGC CCCTCCCACC CAATGTGGGA TTCCTGCACG CAGCACCCCC CAGTGGCCGC
    CGGAGGAGGA TCAGCAAAAA TAAGCTGAAA ACTGGGAAAC TGCTCAATCC GCTGGTGCAG
    CAGCTGAAGA ATTCGACTAC CAGTCCAAGC CGCATTTCCC CAGAATGCAA TGGTTCCCCT
    GCTAAGGATC CTGCAAAGTC TCTGGCAGAC TGCACCGCAG GAACCTCGCC AAGCCGTGCC
    GTACAAACGC AGCAGCCTTG CTCTGTGAAA GTCACCATGT CGTCCGATAA TATCTGCTCT
    GGGGTCACCC GGGACGGTGT CGATGTGCAG AGCAATTCAA GCCAAGGATC TTCGACAAGC
    AGCCTCGCAA CCGCCCCCGA GGCCTCGGCG CTGCCCCCGC TGAACGCACA AATCCAGCCG
    CAGGCCAACA ACAGCAACAA CAACAAAAAG GGGACGTTTA CGGATGACCT TCACAAATTG
    GTGGATGAGT GGGCCTGCAA AACCGTCGGC GTGGCACAAT TCAAACCCTC ATTAAACCAA
    TTGAAGCAGT ACCAGCAGCG GCAAGATCTG GAGCCCAAGT CTGTGCCCAG CGACCTGTGT
    GACATGAGCC GTGCCGTGCC AGAGCCCAGG AAGAGATACA GCATTCCCAT GGCTGCCCCT
    CCGGCTGTAC CACTGGCTCC CCTCCTCTCT GCCGCTGTCC CAGCAACTGT TCCAGGAGCC
    GTGCAGCCGG GTACCTTTGT CATGCCCTTT TGTCCGTACG CGGCACCAAT GTACGGCATG
    CCCTGGGCAG GAGCGGCTGA GCTTGGGACT GCAGGAAATC AGATCATGGC CCAAGTAGCC
    CCCATAGGCA ACCAAATATT CCCTGTGGCA CTGCAGCACC CAGGTTTGCA CCCTGCTGGA
    TCCAACATGC GGATTACGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.