pde6b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pde6b
Entrez Gene ID	100487945
Full Name	phosphodiesterase 6B
General protein information	Preferred Names phosphodiesterase 6B Names rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta phosphodiesterase 6B, cGMP-specific, rod, beta
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012953282.2	XP_012808736.2	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
XM_012953282.3	XP_012808736.2	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pde6b	NP_032832.2
Rattus norvegicus (Norway rat)	Pde6b	NP_001099494.1
Danio rerio (zebrafish)	pde6b	XP_685002.1
Bos taurus (cattle)	PDE6B	NP_776843.1
Xenopus tropicalis (tropical clawed frog)	LOC100487945	XP_002935501.2
Canis lupus familiaris (dog)	PDE6B	NP_001002934.1
Homo sapiens (human)	PDE6B	NP_000274.2
	PDE6B	XP_001094732.2
Gallus gallus (chicken)	PDE6B	XP_424876.3

Gene Ontology

The following pde6b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pde6b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa42350	XM_012953282.2 Latest version!	Xenopus tropicalis phosphodiesterase 6B (pde6b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa48308	XM_012953282.3 Latest version!	Xenopus tropicalis phosphodiesterase 6B (pde6b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pde6b ( XM_012953282.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808736.2 Xenopus tropicalis phosphodiesterase 6B (pde6b), mRNA.

Price & Availability↑

CloneID	OXa42350
Clone ID Related Accession (Same CDS sequence)	XM_012953282.2
Accession Version	XM_012953282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953282.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCA CCAAAGCAAG TCTTACTTTA GAGGATGTGG CAAATTATAT GGATAACAAC 
CCTGCTTTTA CACAAAGATA TTTTGAGAAG AAACTTAAGC CAGAAACTAT TGCTGCTGCC 
TTTGGTCGGA AAGATCTCAC TGTTGATTTT GCGACATTTA GGGAACTCGT CCAGGTTGAA 
GAGAGTGAGA TTCTCTTTGA ACTTGTTAAC GACATGCAAG AAAGTATTAA CATGGAGAAA 
GTTGTGTTCA AGACCCTGAG GAGAATCAGT TTTCTTATCC ACGCAGATCG ATGTAGTCTC 
TTTATGTACA GGCAAAGAAA TGGAACACCT GAGTTAGCTA CAAGACTATT TAATGTTAAG 
TCTAACAGTA AACTGGAAGA ATGCCTTGTT CCACCTGATA GTGAAATAGT TTATCCTTTG 
GACATTGGAA TTGTAGGACA CGTTGCTCAT ACAAAGAAAA CAATTAACGT CAAAGATGTT 
AGCGAGTGCA CAGTGTACAG CAAATTTGCA GATGAACTGA CGGACTATGA GACAAAAAAC 
ATACTTGCCA CACCAATCCT GAATGGTAAA GATGTTGTTG CAGTGATAAT GGCAGTTAAT 
AAACTCGACG GCCCATTTTT TACAAACTAC GATGAAGAGG TATTTTTAAA GTATCTGAAT 
TTTGCCTCTT TAAATCTGAA AATTTATCAC CTAAGCTACT TACATAACTG TGAGACTCGA 
AGAGGCCAGG TACTATTATG GTCAGCAAAC AAAGTGTTTG AAGAGCTGAC TGACATCGAG 
AGACAGTTTC ACAAGGCCTT CTATACAGTG AGGGCATACC TAAATTGTGA CAGATATTCT 
GTTGGCCTCC TTGATATGAC AAAGGAAAAG GAGTTTTTTG ATGTTTGGCC AGTTCTGATG 
GGAGAGGTTC CCCCATACAC AGGACCCCGC ACTCCTGACG GAAGAGAAAT TGTATTTTAC 
AAAGTCATTG ATTATATTCT ACATGGGAAA GAAGATATTA AAGTCATACC AAATCCCCCA 
GCAGATCATT GGGCTTTGGT TAGTGGTCTT CCAACATACG TGGCTGAGAG TGGCTTTATC 
TGTAATATTA TGAATGCAGG TGCAGATGAA ATGTTCAAAT TTCAGAAAGA ACCATTGGAT 
GAAAGTGGAT GGGTTGTTAA AAATGTCCTA TCAATGCCCA TAGTCAACAA GAAGGAGGAA 
ATTGTTGGTG TTGCAACCTT TTACAACAGA AAAGACGGAA AGCCGTTTGA TGAACAGGAT 
GAAACACTGA TGGAATCTCT GACCCAGTTC CTTGGTTGGT CAGTACTAAA TACAGATACG 
CATGACAAGA TGAACAAGCT GGAAAACCGT AAGGATATTG CTCAAGACAT GGTGATGTAC 
CATGTTAAAT GTGACAAGGA TGAAATTCAG GATATCCTGC CTACAAGAGA AAAGCTGGGA 
AAAGAAGTCA CAGAATGTGA GGAGGATGAA ATTGCAAAAA CACTTAAACA GGTCTTACCA 
GGACCTACTA AATTTGAAAT TTATGAGTTT CGTTTCTCAG ACTTTGACTG TACCGAAATT 
GATCTTGTGA AATGCGGTAT CCAAATGTAT TATGAACTTG GAGTTGTAAA AAAGTTTCAG 
GTTCCCCCAG AGGCTTTGGT GAGGTTTATG TACGCAATGA AGAAAGGATA TAGAAGAGTT 
ACCTATCACA ACTGGCGTCA TGGATTTAAT GTGGCACAGA CTATGTTTAC CCTTTTAATG 
ACAGGCAAAC TGAAACGCTA TTATACAGAT CTAGAGGCGA TGGCAATGGT TACTGCTGGC 
TTTTGTCATG ATATAGATCA CAGAGGGACT AACAACCTTT ATCAGATGAA ATCTCAACAT 
CCTCTAGCAA AATTACATGG TTCATCAATT CTAGAAAGAC ATCACTTGGA GTTTGGAAAG 
TTTCTGTTAG CAGATGAGTC AATGAACATA TTTCAGAATC TTAACAGAAG ACAGCATGAA 
CATGTGATTC ATCTTATGGA TATAGCTATT ATAGCTACAG ACTTGGCACT TTATTTCAAA 
AAGCGAACAA TGTTTCAGAA GATTGTTGAC CAATCAAAAA CGTATGAAGA TGAGCAAAAG 
TGGACTGAAT ATTTATCTTT GGAAACAACA AGGAAAGAAA TTGTCATGGC CATGATGATG 
ACAGCATGTG ATTTATCTGC TATTACCAAG CCCTGGGAAG TCCAAAGCAA GGTTGCGCTC 
TTAGTGGCAG CTGAATTCTG GGAACAGGGA GATCTGGAAA GAACAGTTCT TCAACAGCAG 
CCTATTCCCA TGATGGACAG AAATAAGTCT GCAGAGCTGC CAAAACTTCA GTGTGGTTTT 
ATTGACTTTG TGTGCACTTT TGTATATAAG GAATTTGCAC GCTTCCATGA AGAAATACAG 
CCAATGTTAG ATGGCTTGTT AAACAACAGA AAGGAGTGGA AGGCTCTTGC TGATGAATAC 
GAGGCCAAAG TCAAAACAGT CGAAGAGGAG AAGAAAAAGA GGGAAGAAGA ACTTGCTGCA 
CGGAAAGCTG CTGCAGCTGG GGCAAACGCT GCCAAATCTT CAACCTGTTC TATATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808736.2
CDS	1..2580
Translation	MKSTKASLTLEDVANYMDNNPAFTQRYFEKKLKPETIAAAFGRKDLTVDFATFRELVQVEESEILFELVNDMQESINMEKVVFKTLRRISFLIHADRCSLFMYRQRNGTPELATRLFNVKSNSKLEECLVPPDSEIVYPLDIGIVGHVAHTKKTINVKDVSECTVYSKFADELTDYETKNILATPILNGKDVVAVIMAVNKLDGPFFTNYDEEVFLKYLNFASLNLKIYHLSYLHNCETRRGQVLLWSANKVFEELTDIERQFHKAFYTVRAYLNCDRYSVGLLDMTKEKEFFDVWPVLMGEVPPYTGPRTPDGREIVFYKVIDYILHGKEDIKVIPNPPADHWALVSGLPTYVAESGFICNIMNAGADEMFKFQKEPLDESGWVVKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDEQDETLMESLTQFLGWSVLNTDTHDKMNKLENRKDIAQDMVMYHVKCDKDEIQDILPTREKLGKEVTECEEDEIAKTLKQVLPGPTKFEIYEFRFSDFDCTEIDLVKCGIQMYYELGVVKKFQVPPEALVRFMYAMKKGYRRVTYHNWRHGFNVAQTMFTLLMTGKLKRYYTDLEAMAMVTAGFCHDIDHRGTNNLYQMKSQHPLAKLHGSSILERHHLEFGKFLLADESMNIFQNLNRRQHEHVIHLMDIAIIATDLALYFKKRTMFQKIVDQSKTYEDEQKWTEYLSLETTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLQQQPIPMMDRNKSAELPKLQCGFIDFVCTFVYKEFARFHEEIQPMLDGLLNNRKEWKALADEYEAKVKTVEEEKKKREEELAARKAAAAGANAAKSSTCSIC

Target ORF information:

RefSeq Version	XM_012953282.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 6B (pde6b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953282.2

ORF Insert Sequence:

ATGAAATCCA CCAAAGCAAG TCTTACTTTA GAGGATGTGG CAAATTATAT GGATAACAAC 
CCTGCTTTTA CACAAAGATA TTTTGAGAAG AAACTTAAGC CAGAAACTAT TGCTGCTGCC 
TTTGGTCGGA AAGATCTCAC TGTTGATTTT GCGACATTTA GGGAACTCGT CCAGGTTGAA 
GAGAGTGAGA TTCTCTTTGA ACTTGTTAAC GACATGCAAG AAAGTATTAA CATGGAGAAA 
GTTGTGTTCA AGACCCTGAG GAGAATCAGT TTTCTTATCC ACGCAGATCG ATGTAGTCTC 
TTTATGTACA GGCAAAGAAA TGGAACACCT GAGTTAGCTA CAAGACTATT TAATGTTAAG 
TCTAACAGTA AACTGGAAGA ATGCCTTGTT CCACCTGATA GTGAAATAGT TTATCCTTTG 
GACATTGGAA TTGTAGGACA CGTTGCTCAT ACAAAGAAAA CAATTAACGT CAAAGATGTT 
AGCGAGTGCA CAGTGTACAG CAAATTTGCA GATGAACTGA CGGACTATGA GACAAAAAAC 
ATACTTGCCA CACCAATCCT GAATGGTAAA GATGTTGTTG CAGTGATAAT GGCAGTTAAT 
AAACTCGACG GCCCATTTTT TACAAACTAC GATGAAGAGG TATTTTTAAA GTATCTGAAT 
TTTGCCTCTT TAAATCTGAA AATTTATCAC CTAAGCTACT TACATAACTG TGAGACTCGA 
AGAGGCCAGG TACTATTATG GTCAGCAAAC AAAGTGTTTG AAGAGCTGAC TGACATCGAG 
AGACAGTTTC ACAAGGCCTT CTATACAGTG AGGGCATACC TAAATTGTGA CAGATATTCT 
GTTGGCCTCC TTGATATGAC AAAGGAAAAG GAGTTTTTTG ATGTTTGGCC AGTTCTGATG 
GGAGAGGTTC CCCCATACAC AGGACCCCGC ACTCCTGACG GAAGAGAAAT TGTATTTTAC 
AAAGTCATTG ATTATATTCT ACATGGGAAA GAAGATATTA AAGTCATACC AAATCCCCCA 
GCAGATCATT GGGCTTTGGT TAGTGGTCTT CCAACATACG TGGCTGAGAG TGGCTTTATC 
TGTAATATTA TGAATGCAGG TGCAGATGAA ATGTTCAAAT TTCAGAAAGA ACCATTGGAT 
GAAAGTGGAT GGGTTGTTAA AAATGTCCTA TCAATGCCCA TAGTCAACAA GAAGGAGGAA 
ATTGTTGGTG TTGCAACCTT TTACAACAGA AAAGACGGAA AGCCGTTTGA TGAACAGGAT 
GAAACACTGA TGGAATCTCT GACCCAGTTC CTTGGTTGGT CAGTACTAAA TACAGATACG 
CATGACAAGA TGAACAAGCT GGAAAACCGT AAGGATATTG CTCAAGACAT GGTGATGTAC 
CATGTTAAAT GTGACAAGGA TGAAATTCAG GATATCCTGC CTACAAGAGA AAAGCTGGGA 
AAAGAAGTCA CAGAATGTGA GGAGGATGAA ATTGCAAAAA CACTTAAACA GGTCTTACCA 
GGACCTACTA AATTTGAAAT TTATGAGTTT CGTTTCTCAG ACTTTGACTG TACCGAAATT 
GATCTTGTGA AATGCGGTAT CCAAATGTAT TATGAACTTG GAGTTGTAAA AAAGTTTCAG 
GTTCCCCCAG AGGCTTTGGT GAGGTTTATG TACGCAATGA AGAAAGGATA TAGAAGAGTT 
ACCTATCACA ACTGGCGTCA TGGATTTAAT GTGGCACAGA CTATGTTTAC CCTTTTAATG 
ACAGGCAAAC TGAAACGCTA TTATACAGAT CTAGAGGCGA TGGCAATGGT TACTGCTGGC 
TTTTGTCATG ATATAGATCA CAGAGGGACT AACAACCTTT ATCAGATGAA ATCTCAACAT 
CCTCTAGCAA AATTACATGG TTCATCAATT CTAGAAAGAC ATCACTTGGA GTTTGGAAAG 
TTTCTGTTAG CAGATGAGTC AATGAACATA TTTCAGAATC TTAACAGAAG ACAGCATGAA 
CATGTGATTC ATCTTATGGA TATAGCTATT ATAGCTACAG ACTTGGCACT TTATTTCAAA 
AAGCGAACAA TGTTTCAGAA GATTGTTGAC CAATCAAAAA CGTATGAAGA TGAGCAAAAG 
TGGACTGAAT ATTTATCTTT GGAAACAACA AGGAAAGAAA TTGTCATGGC CATGATGATG 
ACAGCATGTG ATTTATCTGC TATTACCAAG CCCTGGGAAG TCCAAAGCAA GGTTGCGCTC 
TTAGTGGCAG CTGAATTCTG GGAACAGGGA GATCTGGAAA GAACAGTTCT TCAACAGCAG 
CCTATTCCCA TGATGGACAG AAATAAGTCT GCAGAGCTGC CAAAACTTCA GTGTGGTTTT 
ATTGACTTTG TGTGCACTTT TGTATATAAG GAATTTGCAC GCTTCCATGA AGAAATACAG 
CCAATGTTAG ATGGCTTGTT AAACAACAGA AAGGAGTGGA AGGCTCTTGC TGATGAATAC 
GAGGCCAAAG TCAAAACAGT CGAAGAGGAG AAGAAAAAGA GGGAAGAAGA ACTTGCTGCA 
CGGAAAGCTG CTGCAGCTGG GGCAAACGCT GCCAAATCTT CAACCTGTTC TATATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde6b ( XM_012953282.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808736.2 Xenopus tropicalis phosphodiesterase 6B (pde6b), mRNA.

Price & Availability↑

CloneID	OXa48308
Clone ID Related Accession (Same CDS sequence)	XM_012953282.3
Accession Version	XM_012953282.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012953282.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCA CCAAAGCAAG TCTTACTTTA GAGGATGTGG CAAATTATAT GGATAACAAC 
CCTGCTTTTA CACAAAGATA TTTTGAGAAG AAACTTAAGC CAGAAACTAT TGCTGCTGCC 
TTTGGTCGGA AAGATCTCAC CGTTGATTTT GCGACATTTA GGGAACTCGT CCAGGTTGAA 
GAGAGTGAGA TTCTCTTTGA ACTTGTTAAC GACATGCAAG AAAGTATTAA CATGGAGAAA 
GTTGTGTTCA AGACCCTGAG GAGAATCAGT TTTCTTATCC ACGCAGATCG ATGTAGTCTC 
TTTATGTACA GGCAAAGAAA TGGAACACCT GAGTTAGCTA CAAGACTATT TAATGTTAAG 
TCTAACAGTA AACTGGAAGA ATGCCTTGTT CCACCTGATA GTGAAATAGT TTATCCTTTG 
GACATTGGAA TTGTAGGACA CGTTGCTCAT ACAAAGAAAA CAATTAACGT CAAAGATGTT 
AGCGAGTGCA CAGTGTACAG CAAATTTGCA GATGAACTGA CGGACTATGA GACAAAAAAC 
ATACTTGCCA CACCAATCCT GAATGGTAAA GATGTTGTTG CAGTGATAAT GGCAGTTAAT 
AAACTCGACG GCCCATTTTT TACAAACTAC GATGAAGAGG TATTTTTAAA GTATCTGAAT 
TTTGCCTCTT TAAATCTGAA AATTTATCAC CTAAGCTACT TACATAACTG TGAGACTCGA 
AGAGGCCAGG TACTATTATG GTCAGCAAAC AAAGTGTTTG AAGAGCTGAC TGACATCGAG 
AGACAGTTTC ACAAGGCCTT CTATACAGTG AGGGCATACC TAAATTGTGA CAGATATTCT 
GTTGGCCTCC TTGATATGAC AAAGGAAAAG GAGTTTTTTG ATGTTTGGCC AGTTCTGATG 
GGAGAGGTTC CCCCATACAC AGGACCCCGC ACTCCTGACG GAAGAGAAAT TGTATTTTAC 
AAAGTCATTG ATTATATTCT ACATGGGAAA GAAGATATTA AAGTCATACC AAATCCCCCA 
GCAGATCATT GGGCTTTGGT TAGTGGTCTT CCAACATACG TGGCTGAGAG TGGCTTTATC 
TGTAATATTA TGAATGCAGG TGCAGATGAA ATGTTCAAAT TTCAGAAAGA ACCATTGGAT 
GAAAGTGGAT GGGTTGTTAA AAATGTCCTA TCAATGCCCA TAGTCAACAA GAAGGAGGAA 
ATTGTTGGTG TTGCAACCTT TTACAACAGA AAAGACGGAA AGCCGTTTGA TGAACAGGAT 
GAAACACTGA TGGAATCTCT GACCCAGTTC CTTGGTTGGT CAGTACTAAA TACAGATACG 
CATGACAAGA TGAACAAGCT GGAAAACCGT AAGGATATTG CTCAAGACAT GGTGATGTAC 
CATGTTAAAT GTGACAAGGA TGAAATTCAG GATATCCTGC CTACAAGAGA AAAGCTGGGA 
AAAGAAGTCA CAGAATGTGA GGAGGATGAA ATTGCAAAAA CACTTAAACA GGTCTTACCA 
GGACCTACTA AATTTGAAAT TTATGAGTTT CGTTTCTCAG ACTTTGACTG TACCGAAATT 
GATCTTGTGA AATGCGGTAT CCAAATGTAT TATGAACTTG GAGTTGTAAA AAAGTTTCAG 
GTTCCCCCAG AGGCTTTGGT GAGGTTTATG TACGCAATGA AGAAAGGATA TAGAAGAGTT 
ACCTATCACA ACTGGCGTCA TGGATTTAAT GTGGCACAGA CTATGTTTAC CCTTTTAATG 
ACAGGCAAAC TGAAACGCTA TTATACAGAT CTAGAGGCGA TGGCAATGGT TACTGCTGGC 
TTTTGTCATG ATATAGATCA CAGAGGGACT AACAACCTTT ATCAGATGAA ATCTCAACAT 
CCTCTAGCAA AATTACATGG TTCATCAATT CTAGAAAGAC ATCACTTGGA GTTTGGAAAG 
TTTCTGTTAG CAGATGAGTC AATGAACATA TTTCAGAATC TTAACAGAAG ACAGCATGAA 
CATGTGATTC ATCTTATGGA TATAGCTATT ATAGCTACAG ACTTGGCACT TTATTTCAAA 
AAGCGAACAA TGTTTCAGAA GATTGTTGAC CAATCAAAAA CGTATGAAGA TGAGCAAAAG 
TGGACTGAAT ATTTATCTTT GGAAACAACA AGGAAAGAAA TTGTCATGGC CATGATGATG 
ACAGCATGTG ATTTATCTGC TATTACCAAG CCCTGGGAAG TCCAGAGCAA GGTTGCGCTC 
TTAGTGGCAG CTGAATTCTG GGAACAGGGA GATCTGGAAA GAACAGTTCT TCAACAGCAG 
CCTATTCCCA TGATGGACAG AAATAAGTCT GCAGAGCTGC CAAAACTTCA GTGTGGTTTT 
ATTGACTTTG TGTGCACTTT TGTATATAAG GAATTTGCAC GCTTCCATGA AGAAATACAG 
CCAATGTTAG ATGGCTTGTT AAACAACAGA AAGGAGTGGA AGGCTCTTGC TGATGAATAC 
GAGGCCAAAG TCAAAACAGT CGAAGAGGAG AAGAAAAAGA GGGAAGAAGA ACTTGCTGCA 
CGGAAAGCTG CTGCAGCTGG GGCAAACGCT GCCAAATCTT CAACCTGTTC TATATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808736.2
CDS	569..3148
Translation	MKSTKASLTLEDVANYMDNNPAFTQRYFEKKLKPETIAAAFGRKDLTVDFATFRELVQVEESEILFELVNDMQESINMEKVVFKTLRRISFLIHADRCSLFMYRQRNGTPELATRLFNVKSNSKLEECLVPPDSEIVYPLDIGIVGHVAHTKKTINVKDVSECTVYSKFADELTDYETKNILATPILNGKDVVAVIMAVNKLDGPFFTNYDEEVFLKYLNFASLNLKIYHLSYLHNCETRRGQVLLWSANKVFEELTDIERQFHKAFYTVRAYLNCDRYSVGLLDMTKEKEFFDVWPVLMGEVPPYTGPRTPDGREIVFYKVIDYILHGKEDIKVIPNPPADHWALVSGLPTYVAESGFICNIMNAGADEMFKFQKEPLDESGWVVKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDEQDETLMESLTQFLGWSVLNTDTHDKMNKLENRKDIAQDMVMYHVKCDKDEIQDILPTREKLGKEVTECEEDEIAKTLKQVLPGPTKFEIYEFRFSDFDCTEIDLVKCGIQMYYELGVVKKFQVPPEALVRFMYAMKKGYRRVTYHNWRHGFNVAQTMFTLLMTGKLKRYYTDLEAMAMVTAGFCHDIDHRGTNNLYQMKSQHPLAKLHGSSILERHHLEFGKFLLADESMNIFQNLNRRQHEHVIHLMDIAIIATDLALYFKKRTMFQKIVDQSKTYEDEQKWTEYLSLETTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLQQQPIPMMDRNKSAELPKLQCGFIDFVCTFVYKEFARFHEEIQPMLDGLLNNRKEWKALADEYEAKVKTVEEEKKKREEELAARKAAAAGANAAKSSTCSIC

Target ORF information:

RefSeq Version	XM_012953282.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis phosphodiesterase 6B (pde6b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953282.3

ORF Insert Sequence:

ATGAAATCCA CCAAAGCAAG TCTTACTTTA GAGGATGTGG CAAATTATAT GGATAACAAC 
CCTGCTTTTA CACAAAGATA TTTTGAGAAG AAACTTAAGC CAGAAACTAT TGCTGCTGCC 
TTTGGTCGGA AAGATCTCAC CGTTGATTTT GCGACATTTA GGGAACTCGT CCAGGTTGAA 
GAGAGTGAGA TTCTCTTTGA ACTTGTTAAC GACATGCAAG AAAGTATTAA CATGGAGAAA 
GTTGTGTTCA AGACCCTGAG GAGAATCAGT TTTCTTATCC ACGCAGATCG ATGTAGTCTC 
TTTATGTACA GGCAAAGAAA TGGAACACCT GAGTTAGCTA CAAGACTATT TAATGTTAAG 
TCTAACAGTA AACTGGAAGA ATGCCTTGTT CCACCTGATA GTGAAATAGT TTATCCTTTG 
GACATTGGAA TTGTAGGACA CGTTGCTCAT ACAAAGAAAA CAATTAACGT CAAAGATGTT 
AGCGAGTGCA CAGTGTACAG CAAATTTGCA GATGAACTGA CGGACTATGA GACAAAAAAC 
ATACTTGCCA CACCAATCCT GAATGGTAAA GATGTTGTTG CAGTGATAAT GGCAGTTAAT 
AAACTCGACG GCCCATTTTT TACAAACTAC GATGAAGAGG TATTTTTAAA GTATCTGAAT 
TTTGCCTCTT TAAATCTGAA AATTTATCAC CTAAGCTACT TACATAACTG TGAGACTCGA 
AGAGGCCAGG TACTATTATG GTCAGCAAAC AAAGTGTTTG AAGAGCTGAC TGACATCGAG 
AGACAGTTTC ACAAGGCCTT CTATACAGTG AGGGCATACC TAAATTGTGA CAGATATTCT 
GTTGGCCTCC TTGATATGAC AAAGGAAAAG GAGTTTTTTG ATGTTTGGCC AGTTCTGATG 
GGAGAGGTTC CCCCATACAC AGGACCCCGC ACTCCTGACG GAAGAGAAAT TGTATTTTAC 
AAAGTCATTG ATTATATTCT ACATGGGAAA GAAGATATTA AAGTCATACC AAATCCCCCA 
GCAGATCATT GGGCTTTGGT TAGTGGTCTT CCAACATACG TGGCTGAGAG TGGCTTTATC 
TGTAATATTA TGAATGCAGG TGCAGATGAA ATGTTCAAAT TTCAGAAAGA ACCATTGGAT 
GAAAGTGGAT GGGTTGTTAA AAATGTCCTA TCAATGCCCA TAGTCAACAA GAAGGAGGAA 
ATTGTTGGTG TTGCAACCTT TTACAACAGA AAAGACGGAA AGCCGTTTGA TGAACAGGAT 
GAAACACTGA TGGAATCTCT GACCCAGTTC CTTGGTTGGT CAGTACTAAA TACAGATACG 
CATGACAAGA TGAACAAGCT GGAAAACCGT AAGGATATTG CTCAAGACAT GGTGATGTAC 
CATGTTAAAT GTGACAAGGA TGAAATTCAG GATATCCTGC CTACAAGAGA AAAGCTGGGA 
AAAGAAGTCA CAGAATGTGA GGAGGATGAA ATTGCAAAAA CACTTAAACA GGTCTTACCA 
GGACCTACTA AATTTGAAAT TTATGAGTTT CGTTTCTCAG ACTTTGACTG TACCGAAATT 
GATCTTGTGA AATGCGGTAT CCAAATGTAT TATGAACTTG GAGTTGTAAA AAAGTTTCAG 
GTTCCCCCAG AGGCTTTGGT GAGGTTTATG TACGCAATGA AGAAAGGATA TAGAAGAGTT 
ACCTATCACA ACTGGCGTCA TGGATTTAAT GTGGCACAGA CTATGTTTAC CCTTTTAATG 
ACAGGCAAAC TGAAACGCTA TTATACAGAT CTAGAGGCGA TGGCAATGGT TACTGCTGGC 
TTTTGTCATG ATATAGATCA CAGAGGGACT AACAACCTTT ATCAGATGAA ATCTCAACAT 
CCTCTAGCAA AATTACATGG TTCATCAATT CTAGAAAGAC ATCACTTGGA GTTTGGAAAG 
TTTCTGTTAG CAGATGAGTC AATGAACATA TTTCAGAATC TTAACAGAAG ACAGCATGAA 
CATGTGATTC ATCTTATGGA TATAGCTATT ATAGCTACAG ACTTGGCACT TTATTTCAAA 
AAGCGAACAA TGTTTCAGAA GATTGTTGAC CAATCAAAAA CGTATGAAGA TGAGCAAAAG 
TGGACTGAAT ATTTATCTTT GGAAACAACA AGGAAAGAAA TTGTCATGGC CATGATGATG 
ACAGCATGTG ATTTATCTGC TATTACCAAG CCCTGGGAAG TCCAGAGCAA GGTTGCGCTC 
TTAGTGGCAG CTGAATTCTG GGAACAGGGA GATCTGGAAA GAACAGTTCT TCAACAGCAG 
CCTATTCCCA TGATGGACAG AAATAAGTCT GCAGAGCTGC CAAAACTTCA GTGTGGTTTT 
ATTGACTTTG TGTGCACTTT TGTATATAAG GAATTTGCAC GCTTCCATGA AGAAATACAG 
CCAATGTTAG ATGGCTTGTT AAACAACAGA AAGGAGTGGA AGGCTCTTGC TGATGAATAC 
GAGGCCAAAG TCAAAACAGT CGAAGAGGAG AAGAAAAAGA GGGAAGAAGA ACTTGCTGCA 
CGGAAAGCTG CTGCAGCTGG GGCAAACGCT GCCAAATCTT CAACCTGTTC TATATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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pde6b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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