dip2b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	dip2b
Entrez Gene ID	100487550
Full Name	disco interacting protein 2 homolog B
General protein information	Preferred Names disco interacting protein 2 homolog B Names disco-interacting protein 2 homolog B DIP2 disco-interacting protein 2 homolog B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002933708.4	XP_002933754.2	disco-interacting protein 2 homolog B isoform X1
XM_004911909.3	XP_004911966.1	disco-interacting protein 2 homolog B isoform X2
XM_018091708.1	XP_017947197.1	disco-interacting protein 2 homolog B isoform X3
XM_002933708.5	XP_002933754.2	disco-interacting protein 2 homolog B isoform X1
XM_004911909.4	XP_004911966.1	disco-interacting protein 2 homolog B isoform X2
XM_018091708.2	XP_017947197.1	disco-interacting protein 2 homolog B isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DIP2B	NP_001179238.1
Rattus norvegicus (Norway rat)	Dip2b	XP_006257470.1
Xenopus tropicalis (tropical clawed frog)	dip2b	XP_004911966.1
Pan troglodytes (chimpanzee)	DIP2B	XP_509060.2
Gallus gallus (chicken)	DIP2B	XP_424482.3
Canis lupus familiaris (dog)	DIP2B	XP_005636894.1
Homo sapiens (human)	DIP2B	NP_775873.2
	DIP2B	XP_001082866.2
Danio rerio (zebrafish)	dip2ba	NP_998128.1
Danio rerio (zebrafish)	LOC568954	XP_002662549.2
Mus musculus (house mouse)	Dip2b	NP_001152833.1

Gene Ontology

The following dip2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dip2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa42851	XM_018091708.1 Latest version!	Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa52712	XM_002933708.5 Latest version!	Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa52713	XM_004911909.4 Latest version!	Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa52714	XM_018091708.2 Latest version!	Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa06062	XM_002933708.4 Latest version!	Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa06063	XM_004911909.3 Latest version!	Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dip2b ( XM_018091708.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947197.1 Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa42851
Clone ID Related Accession (Same CDS sequence)	XM_018091708.1
Accession Version	XM_018091708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	disco-interacting protein 2 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGA GAGCCTTCGA CCTATCCGCC CTCCCGAAGG AGGTGAGGGA GCAGCTGGCA 
GAACTGGAGC TGGAGCTGTC AGAAGGTGTT GATCCAGCGC TACAGAAAGA GAATCGGAAC 
CAAACAACTG GATCACCTGC TGCTCCTGCG CAATCGTCCT CTAAGTACCA TCGAGCCAGA 
TCCGGAGGAG CTAGAGATGA GCGTTACAGA TCAGATATCC ATACAGAAGC AGTCCAAGCG 
GCTCTGGCTA AGCATAAAGA ACAGAAGATG GCATTACCAA TGCCAACCAA AAGGCGGTCA 
ACATTTGTGC AGTCTCCAGC TGACGCATGT ACCCCTCCAG ATACATCCTC CGCATCTGAG 
GACGAAGGAT CCCTAAGACG CCAGGCTGCT CTTTCGGCAG CTCTTGCTCA GAGTTTACAG 
AATTCAGAGG CCTGGATCAA CAGGTCCGTT CAGGGTTCAT CCACATCATC TTCAGCATCC 
TCCACATTAT CGCACGGAGA AGTAAAGAGT ACCAGTGGCA CCTTAGCAGA TATACTTTCT 
AATACTCACA TAGACAGTTC TCCTGCTCCA CCCGATGTTA CCAACACCAC CAATTCCACA 
ACCACTACAA GCACATCATC GTCTTCGTCA TCCTTGTCTT CAGTTCGCCC AGCAACCATT 
GACCTTCCCC CTGCAGGCCT GGTGAAAGGG GTGCACAAGG CAGCTAGAAG GTCTAGCTTA 
ATGGATACTG CAGATGGTGT TGCAGTGAAC AGTCGCGTCT CAACAAAAAT CCAGCAGTTG 
CTGAACACGC TCAAGCGACC AAAAAGGCCA CCATTAAAGG AATTTTTTGT GGATGATTTT 
GAAGAGATTG TAGAAGTACC CCGACCAGAC CCAAACCAGC CCAAACCAGA AGGTCGCCAG 
GTGACCCCTG TAAAAGGAGA GCCCCTGGGT GTTGTGTGCA ACTGGCCCCC GTCGGTAGAA 
GCCGCACTGC AGCGCTGGGG AACAACACAA GCAAAATGTT CATGTCTTAC CGCTCTTGAT 
GTCACTGGCA AACCAACCTA TACGTTAACA TATGGCAAGC TATGGACCAG GAGTGTCAGA 
CTGGCTTATA CCCTGCTGAA CAAATTGGGT ACCAAGAATG AACAAGTGCT GAAGCCTGGG 
GACAGGGTTG CCCTGGTTTA CCCCAATAAT GACCCAGTGA TGTTCATGGT TGCGTTTTAT 
GGCTGTCTCT TGGCGGAGGT GATCCCAGTG CCGATTGAGG TACCTCTTAC TAGAAAGGAC 
GCTGGAGGGC ATCAGATTGG ATTCCTGCTG GGAAGCTGTG GTGTTAAGAT CGCTCTTACT 
ACTGAGGTTT GCTTGAAGGG GCTGCCCAAG ACACAGAACG GGGAGATTGT GCAATTCAAA 
GGTTGGCCAA AACTCAAGTG GGTTGTAACA GACTCCAGAT ACCTATCAAA ATCACCAAAG 
GACTGGCAGC CACACATCAC CAATGCTGGC ACAGAGCCGG CATACATTGA GTACAAAACA 
AGCAAGGAAG GCAGTGTGAT GGGTGTGACC GTGTCTCGGA TAGCCATGCT GTCTCATTGC 
CAGGCACTCT CACAGGCCTG TAACTACACA GAGGGTGAGA CCTTAGTTAA TGTCCTGGAC 
TTCAAGAGGG ATGCAGGCCT GTGGCATGGT ATACTAACGA ATGTAGTAAA TAAAATGCAC 
ACGATCAGCG TGCCGTACGC AGTGATGAAA GCATGTCCGT TGTCCTGGGT GCAGAGGGTA 
CATGCCAGTA AAGGCAGGGT GGCTGTAGTG AAGTGCCGGG ACTTGCACTG GGCCATGATA 
GCCCACCGAG AACAAAAGGA TATCAGTCTG GCCTCTCTCC GTATGCTCAT TGTTACAGAT 
GGAGCTAATC CTTGGTCCGT TTCCTCATGT GATGCATTCC TGAGTTTGTT TCAGAATCAC 
GGCCTGAAGC CTGAGGTCAT CTGTCCATGT GCAACCTCTC CAGAAGCTAT GACTGTCGCT 
ATTCGAAGGC CTGGAGTACC CGGTGCCCCC CTGCCTGGAA GAGCCATTTT GTCTATGAAT 
GGGTTAAGTT ATGGCGTGAT CAGAGTTAAT ACAGAGGACA AGAATTCTGC ATTGACCGTC 
CAGGACGTTG GACATGTTAT GCCAGGAAGT GTGATGTGCA CTGTGAAGCC GGAAGGAGTA 
CCCCAGCTCT GTAAAACTGA TGAGATAGGA GAGATCTGTG TGAGCTGTAA AGGAGGAGGC 
ACAATGTACT ATGGCCTGGC AGGGCTCACC AAGAACACCT TTGAGGTTTT GCCAGTTAAT 
GCTTCTGGGG CTTCTATAGG AGAAATACCC TTCATCAGAA CTGGGCTACT TGGATTTGTG 
GGGCCAGGCA GTTTAGTGTT TGTGGTGGGA AAGATGGACG GCACGGTGAT GGTGAGTGGG 
AGGCGCCACA ATGCTGATGA TATTGTGGCT ACTGCCCTGG CGGTGGAATC CTTTAAAACC 
CTGTACAGAG GAAGGATTGC TGTGTTTTCA GTCACAGTAT TTTATGACGA GCGGATTGTG 
GTGGTGGCAG AACAGCGCCC TGATGCTTCT GAGGAAGATT CCTTCCAATG GATGAGCCGT 
GTATTACAGG CCATTGATAG TATCCACCAA GTGGGAGTTT ACTGTCTTGC CCTGGTTCCA 
GCCAATACGT TGCCAAAAAC TCCACTGGGA GGAATCCACA TATCGCAAAC TAAACAGCTT 
TTCTTGGAGG GCAGCTTACA CCCCTGTAAC ATACTCATGT GTCCTCATAC GTGTGTAACC 
AACTTGCCAA AACCAAGGCA GAAGCAGCCA GGAGTTGGGC CAGCATCTGT CATGGTGGGT 
AATCTGGTCG CAGGAAAAAG AATTGCTCAG GCCTCCGGAA GAGATTTGGG ACAAATAGAG 
GAAAATGATT TGGTGAAAAA GCACCAGTAT CTGACGGAAG TCTTACAGTG GCGAGCGCAA 
ACCACTCCCG AACACGTTCT TTTTATCCTC CTCAATGCAA AGGGAACGGC AGTTTGCAAT 
GCCTCATGTT TACAGCTACA CAAGCGAGCA GAAAGAATAG CAGCTGCCCT ATGTGATAAA 
GGTCATCTCA ACGCCGGCGA TAACGTGGTG CTACTTTTTC CTCCCGGAAT TGAGTTGATA 
GCTGCATTCT ATGGCTGCCT GTACGCTGGC TGTATTCCTG TAACTGTCCG GCCGCCTCAC 
GCACAGAACC TCGCTGCCAC CTTACCTACA GTACGAATGA TTGTAGATGT TAGCAAAGCT 
GCCTGCATTC TCACTACACA AGCATTGCTG AGATTGCTCA AGTCCAAAGA AGCAGCAGCG 
GCTGTGGATG TGAAAACTTG GCCAACCATC ATCGACACCG ATGATTTGCC CAGGAAACGG 
GTACCGCAGC TGTATAAACC ACCTACACCT GAAATGCTGG CTTACCTGGA CTTCAGTGTG 
AGCACGACTG GCATGCTTAC GGGGGTTAAG ATGTCTCATG GATCAGTAAA TGCACTGTGC 
CGGTCCATAA AGCTGCAGTG TGAGCTGTAC TCTACTCGTC AGATCGCCAT TTGTCTTGAT 
CCTTATTGTG GGCTTGGCTT TGTACTTTGG TGCCTTTGTA GTGTTTATTC TGGTCATCAG 
TCTATATTAA TTCCTCCTAT GGAGTTGGAA TCCAACTTAT TCTTGTGGCT CTCTGCTGTG 
AGTCAGTATA AAATTAGGGA CACGTTCTGT TCATATTCTG TGATGGAGAT GTGCTCAAAG 
GGACTGGGGA ACCAGATTGA GGTGCTAAAG ACCAAAGGCA TTAATCTGTC TTACGTGCGG 
ACCTGTGTGG TGGTTGCTGA AGAGCGGCCG AGGGTGGCTT TGGCATATTC CTTTTCAAAA 
CTCTTCAAAG ACCTTGGGCT GTCCCCCCGC GCTGTGAGCA CAACCTTTGG ATCCAGAGTT 
AACACCGCTA TATGCTTGCA GGGAACCTCA GGGCCAGATC CAACAACTGT GTATGTGGAT 
CTGAAATCCC TTCGGCATGA CAGGGTGCGC CTGGTGGAGA GGGGATCTCC GCAGAGTTTG 
CTCCTATTGG AATCTGGAAA GATTTTACCT GGTGTCACCG TAATTATTGT TAATCCAGAG 
ACCAAAGGAC CATTGGGGGA TTCCCACCTT GGAGAGATCT GGGTGAACAG CGCCCACACA 
GCCAGCGGAT ACTACACAAT ATATGATAGT GAGACCTTAC AGGCTGATCA TTTCAATACT 
AGGCTTAGCT TTGGAGACAC ACAGACACTT TGGGCCAAAA CGGGCTATCT TGGCTTTGTC 
CGCAGGACAG AACTGACTGA TGCAAATGGA GAGCGGCACG ATGCGCTGTT TGTGGTTGGA 
GCACTAGATG AAACGCTGGA GCTGAGGGGG CTTCGTTATC ATCCGATAGA TATCGAGACC 
TCAGTGTCAC GCATACACCG GAGTATCGCT GAATGTGCTG TTTTCACATG GACCAACCTG 
CTGGTAGTGG TGGTGGAGCT GTGCGGCTCA GAACAGGAGG CTCTGGACTT GGTTCCTCTG 
GTGACCAACG TGGTGTTGGA AGAACATTAC CTGATTGTGG GCGTGGTGGT GGTGGTGGAT 
CCCGGCGTGA TACCCATCAA TTCCCGGGGA GAGAAACAAC GGATGCACCT GCGGGACAGT 
TTCTTAGCGG ACCAGTTGGA TCCAATCTAC GTGGCGTACA ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947197.1
CDS	204..4871
Translation	MAERAFDLSALPKEVREQLAELELELSEGVDPALQKENRNQTTGSPAAPAQSSSKYHRARSGGARDERYRSDIHTEAVQAALAKHKEQKMALPMPTKRRSTFVQSPADACTPPDTSSASEDEGSLRRQAALSAALAQSLQNSEAWINRSVQGSSTSSSASSTLSHGEVKSTSGTLADILSNTHIDSSPAPPDVTNTTNSTTTTSTSSSSSSLSSVRPATIDLPPAGLVKGVHKAARRSSLMDTADGVAVNSRVSTKIQQLLNTLKRPKRPPLKEFFVDDFEEIVEVPRPDPNQPKPEGRQVTPVKGEPLGVVCNWPPSVEAALQRWGTTQAKCSCLTALDVTGKPTYTLTYGKLWTRSVRLAYTLLNKLGTKNEQVLKPGDRVALVYPNNDPVMFMVAFYGCLLAEVIPVPIEVPLTRKDAGGHQIGFLLGSCGVKIALTTEVCLKGLPKTQNGEIVQFKGWPKLKWVVTDSRYLSKSPKDWQPHITNAGTEPAYIEYKTSKEGSVMGVTVSRIAMLSHCQALSQACNYTEGETLVNVLDFKRDAGLWHGILTNVVNKMHTISVPYAVMKACPLSWVQRVHASKGRVAVVKCRDLHWAMIAHREQKDISLASLRMLIVTDGANPWSVSSCDAFLSLFQNHGLKPEVICPCATSPEAMTVAIRRPGVPGAPLPGRAILSMNGLSYGVIRVNTEDKNSALTVQDVGHVMPGSVMCTVKPEGVPQLCKTDEIGEICVSCKGGGTMYYGLAGLTKNTFEVLPVNASGASIGEIPFIRTGLLGFVGPGSLVFVVGKMDGTVMVSGRRHNADDIVATALAVESFKTLYRGRIAVFSVTVFYDERIVVVAEQRPDASEEDSFQWMSRVLQAIDSIHQVGVYCLALVPANTLPKTPLGGIHISQTKQLFLEGSLHPCNILMCPHTCVTNLPKPRQKQPGVGPASVMVGNLVAGKRIAQASGRDLGQIEENDLVKKHQYLTEVLQWRAQTTPEHVLFILLNAKGTAVCNASCLQLHKRAERIAAALCDKGHLNAGDNVVLLFPPGIELIAAFYGCLYAGCIPVTVRPPHAQNLAATLPTVRMIVDVSKAACILTTQALLRLLKSKEAAAAVDVKTWPTIIDTDDLPRKRVPQLYKPPTPEMLAYLDFSVSTTGMLTGVKMSHGSVNALCRSIKLQCELYSTRQIAICLDPYCGLGFVLWCLCSVYSGHQSILIPPMELESNLFLWLSAVSQYKIRDTFCSYSVMEMCSKGLGNQIEVLKTKGINLSYVRTCVVVAEERPRVALAYSFSKLFKDLGLSPRAVSTTFGSRVNTAICLQGTSGPDPTTVYVDLKSLRHDRVRLVERGSPQSLLLLESGKILPGVTVIIVNPETKGPLGDSHLGEIWVNSAHTASGYYTIYDSETLQADHFNTRLSFGDTQTLWAKTGYLGFVRRTELTDANGERHDALFVVGALDETLELRGLRYHPIDIETSVSRIHRSIAECAVFTWTNLLVVVVELCGSEQEALDLVPLVTNVVLEEHYLIVGVVVVVDPGVIPINSRGEKQRMHLRDSFLADQLDPIYVAYNM

Target ORF information:

RefSeq Version	XM_018091708.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091708.1

ORF Insert Sequence:

ATGGCGGAGA GAGCCTTCGA CCTATCCGCC CTCCCGAAGG AGGTGAGGGA GCAGCTGGCA 
GAACTGGAGC TGGAGCTGTC AGAAGGTGTT GATCCAGCGC TACAGAAAGA GAATCGGAAC 
CAAACAACTG GATCACCTGC TGCTCCTGCG CAATCGTCCT CTAAGTACCA TCGAGCCAGA 
TCCGGAGGAG CTAGAGATGA GCGTTACAGA TCAGATATCC ATACAGAAGC AGTCCAAGCG 
GCTCTGGCTA AGCATAAAGA ACAGAAGATG GCATTACCAA TGCCAACCAA AAGGCGGTCA 
ACATTTGTGC AGTCTCCAGC TGACGCATGT ACCCCTCCAG ATACATCCTC CGCATCTGAG 
GACGAAGGAT CCCTAAGACG CCAGGCTGCT CTTTCGGCAG CTCTTGCTCA GAGTTTACAG 
AATTCAGAGG CCTGGATCAA CAGGTCCGTT CAGGGTTCAT CCACATCATC TTCAGCATCC 
TCCACATTAT CGCACGGAGA AGTAAAGAGT ACCAGTGGCA CCTTAGCAGA TATACTTTCT 
AATACTCACA TAGACAGTTC TCCTGCTCCA CCCGATGTTA CCAACACCAC CAATTCCACA 
ACCACTACAA GCACATCATC GTCTTCGTCA TCCTTGTCTT CAGTTCGCCC AGCAACCATT 
GACCTTCCCC CTGCAGGCCT GGTGAAAGGG GTGCACAAGG CAGCTAGAAG GTCTAGCTTA 
ATGGATACTG CAGATGGTGT TGCAGTGAAC AGTCGCGTCT CAACAAAAAT CCAGCAGTTG 
CTGAACACGC TCAAGCGACC AAAAAGGCCA CCATTAAAGG AATTTTTTGT GGATGATTTT 
GAAGAGATTG TAGAAGTACC CCGACCAGAC CCAAACCAGC CCAAACCAGA AGGTCGCCAG 
GTGACCCCTG TAAAAGGAGA GCCCCTGGGT GTTGTGTGCA ACTGGCCCCC GTCGGTAGAA 
GCCGCACTGC AGCGCTGGGG AACAACACAA GCAAAATGTT CATGTCTTAC CGCTCTTGAT 
GTCACTGGCA AACCAACCTA TACGTTAACA TATGGCAAGC TATGGACCAG GAGTGTCAGA 
CTGGCTTATA CCCTGCTGAA CAAATTGGGT ACCAAGAATG AACAAGTGCT GAAGCCTGGG 
GACAGGGTTG CCCTGGTTTA CCCCAATAAT GACCCAGTGA TGTTCATGGT TGCGTTTTAT 
GGCTGTCTCT TGGCGGAGGT GATCCCAGTG CCGATTGAGG TACCTCTTAC TAGAAAGGAC 
GCTGGAGGGC ATCAGATTGG ATTCCTGCTG GGAAGCTGTG GTGTTAAGAT CGCTCTTACT 
ACTGAGGTTT GCTTGAAGGG GCTGCCCAAG ACACAGAACG GGGAGATTGT GCAATTCAAA 
GGTTGGCCAA AACTCAAGTG GGTTGTAACA GACTCCAGAT ACCTATCAAA ATCACCAAAG 
GACTGGCAGC CACACATCAC CAATGCTGGC ACAGAGCCGG CATACATTGA GTACAAAACA 
AGCAAGGAAG GCAGTGTGAT GGGTGTGACC GTGTCTCGGA TAGCCATGCT GTCTCATTGC 
CAGGCACTCT CACAGGCCTG TAACTACACA GAGGGTGAGA CCTTAGTTAA TGTCCTGGAC 
TTCAAGAGGG ATGCAGGCCT GTGGCATGGT ATACTAACGA ATGTAGTAAA TAAAATGCAC 
ACGATCAGCG TGCCGTACGC AGTGATGAAA GCATGTCCGT TGTCCTGGGT GCAGAGGGTA 
CATGCCAGTA AAGGCAGGGT GGCTGTAGTG AAGTGCCGGG ACTTGCACTG GGCCATGATA 
GCCCACCGAG AACAAAAGGA TATCAGTCTG GCCTCTCTCC GTATGCTCAT TGTTACAGAT 
GGAGCTAATC CTTGGTCCGT TTCCTCATGT GATGCATTCC TGAGTTTGTT TCAGAATCAC 
GGCCTGAAGC CTGAGGTCAT CTGTCCATGT GCAACCTCTC CAGAAGCTAT GACTGTCGCT 
ATTCGAAGGC CTGGAGTACC CGGTGCCCCC CTGCCTGGAA GAGCCATTTT GTCTATGAAT 
GGGTTAAGTT ATGGCGTGAT CAGAGTTAAT ACAGAGGACA AGAATTCTGC ATTGACCGTC 
CAGGACGTTG GACATGTTAT GCCAGGAAGT GTGATGTGCA CTGTGAAGCC GGAAGGAGTA 
CCCCAGCTCT GTAAAACTGA TGAGATAGGA GAGATCTGTG TGAGCTGTAA AGGAGGAGGC 
ACAATGTACT ATGGCCTGGC AGGGCTCACC AAGAACACCT TTGAGGTTTT GCCAGTTAAT 
GCTTCTGGGG CTTCTATAGG AGAAATACCC TTCATCAGAA CTGGGCTACT TGGATTTGTG 
GGGCCAGGCA GTTTAGTGTT TGTGGTGGGA AAGATGGACG GCACGGTGAT GGTGAGTGGG 
AGGCGCCACA ATGCTGATGA TATTGTGGCT ACTGCCCTGG CGGTGGAATC CTTTAAAACC 
CTGTACAGAG GAAGGATTGC TGTGTTTTCA GTCACAGTAT TTTATGACGA GCGGATTGTG 
GTGGTGGCAG AACAGCGCCC TGATGCTTCT GAGGAAGATT CCTTCCAATG GATGAGCCGT 
GTATTACAGG CCATTGATAG TATCCACCAA GTGGGAGTTT ACTGTCTTGC CCTGGTTCCA 
GCCAATACGT TGCCAAAAAC TCCACTGGGA GGAATCCACA TATCGCAAAC TAAACAGCTT 
TTCTTGGAGG GCAGCTTACA CCCCTGTAAC ATACTCATGT GTCCTCATAC GTGTGTAACC 
AACTTGCCAA AACCAAGGCA GAAGCAGCCA GGAGTTGGGC CAGCATCTGT CATGGTGGGT 
AATCTGGTCG CAGGAAAAAG AATTGCTCAG GCCTCCGGAA GAGATTTGGG ACAAATAGAG 
GAAAATGATT TGGTGAAAAA GCACCAGTAT CTGACGGAAG TCTTACAGTG GCGAGCGCAA 
ACCACTCCCG AACACGTTCT TTTTATCCTC CTCAATGCAA AGGGAACGGC AGTTTGCAAT 
GCCTCATGTT TACAGCTACA CAAGCGAGCA GAAAGAATAG CAGCTGCCCT ATGTGATAAA 
GGTCATCTCA ACGCCGGCGA TAACGTGGTG CTACTTTTTC CTCCCGGAAT TGAGTTGATA 
GCTGCATTCT ATGGCTGCCT GTACGCTGGC TGTATTCCTG TAACTGTCCG GCCGCCTCAC 
GCACAGAACC TCGCTGCCAC CTTACCTACA GTACGAATGA TTGTAGATGT TAGCAAAGCT 
GCCTGCATTC TCACTACACA AGCATTGCTG AGATTGCTCA AGTCCAAAGA AGCAGCAGCG 
GCTGTGGATG TGAAAACTTG GCCAACCATC ATCGACACCG ATGATTTGCC CAGGAAACGG 
GTACCGCAGC TGTATAAACC ACCTACACCT GAAATGCTGG CTTACCTGGA CTTCAGTGTG 
AGCACGACTG GCATGCTTAC GGGGGTTAAG ATGTCTCATG GATCAGTAAA TGCACTGTGC 
CGGTCCATAA AGCTGCAGTG TGAGCTGTAC TCTACTCGTC AGATCGCCAT TTGTCTTGAT 
CCTTATTGTG GGCTTGGCTT TGTACTTTGG TGCCTTTGTA GTGTTTATTC TGGTCATCAG 
TCTATATTAA TTCCTCCTAT GGAGTTGGAA TCCAACTTAT TCTTGTGGCT CTCTGCTGTG 
AGTCAGTATA AAATTAGGGA CACGTTCTGT TCATATTCTG TGATGGAGAT GTGCTCAAAG 
GGACTGGGGA ACCAGATTGA GGTGCTAAAG ACCAAAGGCA TTAATCTGTC TTACGTGCGG 
ACCTGTGTGG TGGTTGCTGA AGAGCGGCCG AGGGTGGCTT TGGCATATTC CTTTTCAAAA 
CTCTTCAAAG ACCTTGGGCT GTCCCCCCGC GCTGTGAGCA CAACCTTTGG ATCCAGAGTT 
AACACCGCTA TATGCTTGCA GGGAACCTCA GGGCCAGATC CAACAACTGT GTATGTGGAT 
CTGAAATCCC TTCGGCATGA CAGGGTGCGC CTGGTGGAGA GGGGATCTCC GCAGAGTTTG 
CTCCTATTGG AATCTGGAAA GATTTTACCT GGTGTCACCG TAATTATTGT TAATCCAGAG 
ACCAAAGGAC CATTGGGGGA TTCCCACCTT GGAGAGATCT GGGTGAACAG CGCCCACACA 
GCCAGCGGAT ACTACACAAT ATATGATAGT GAGACCTTAC AGGCTGATCA TTTCAATACT 
AGGCTTAGCT TTGGAGACAC ACAGACACTT TGGGCCAAAA CGGGCTATCT TGGCTTTGTC 
CGCAGGACAG AACTGACTGA TGCAAATGGA GAGCGGCACG ATGCGCTGTT TGTGGTTGGA 
GCACTAGATG AAACGCTGGA GCTGAGGGGG CTTCGTTATC ATCCGATAGA TATCGAGACC 
TCAGTGTCAC GCATACACCG GAGTATCGCT GAATGTGCTG TTTTCACATG GACCAACCTG 
CTGGTAGTGG TGGTGGAGCT GTGCGGCTCA GAACAGGAGG CTCTGGACTT GGTTCCTCTG 
GTGACCAACG TGGTGTTGGA AGAACATTAC CTGATTGTGG GCGTGGTGGT GGTGGTGGAT 
CCCGGCGTGA TACCCATCAA TTCCCGGGGA GAGAAACAAC GGATGCACCT GCGGGACAGT 
TTCTTAGCGG ACCAGTTGGA TCCAATCTAC GTGGCGTACA ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dip2b ( XM_002933708.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933754.2 Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa52712
Clone ID Related Accession (Same CDS sequence)	XM_002933708.5
Accession Version	XM_002933708.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	disco-interacting protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002933708.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGA GAGCCTTCGA CCTATCCGCC CTCCCGAAGG AGGTGAGGGA GCAGCTGGCA 
GAACTGGAGC TGGAGCTGTC AGAAGGTGAT ATCACCCAGA AGGGTTATGA GAAAAAAAGA 
GCAAAACTAT TGGGTCCGTA CATCCCGCAA ACACAAGGTG TTGATCCAGC GCTACAGAAA 
GAGAATCGGA ACCAAACAAC TGGATCACCT GCTGCTCCTG CGCAATCGTC CTCTAAGTAC 
CATCGAGCCA GATCCGGAGG AGCTAGAGAT GAGCGTTACA GATCAGATAT CCATACAGAA 
GCAGTCCAAG CGGCTCTGGC TAAGCATAAA GAACAGAAGA TGGCATTACC AATGCCAACC 
AAAAGGCGGT CAACATTTGT GCAGTCTCCA GCTGACGCAT GTACCCCTCC AGATACATCC 
TCCGCATCTG AGGACGAAGG ATCCCTAAGA CGCCAGGCTG CTCTTTCGGC AGCTCTTGCT 
CAGAGTTTAC AGAATTCAGA GGCCTGGATC AACAGGTCCG TTCAGGGTTC ATCCACATCA 
TCTTCAGCAT CCTCCACATT ATCGCACGGA GAAGTAAAGA GTACCAGTGG CACCTTAGCA 
GATATACTTT CTAATACTCA CATAGACAGT TCTCCTGCTC CACCCGATGT TACCAACACC 
ACCAATTCCA CAACCACTAC AAGCACATCA TCGTCTTCGT CATCCTTGTC TTCAGTTCGC 
CCAGCAACCA TTGACCTTCC CCCTGCAGGC CTGGTGAAAG GGGTGCACAA GGCAGCTAGA 
AGGTCTAGCT TAATGGATAC TGCAGATGGT GTTGCAGTGA ACAGTCGCGT CTCAACAAAA 
ATCCAGCAGT TGCTGAACAC GCTCAAGCGA CCAAAAAGGC CACCATTAAA GGAATTTTTT 
GTGGATGATT TTGAAGAGAT TGTAGAAGTA CCCCGACCAG ACCCAAACCA GCCCAAACCA 
GAAGGTCGCC AGGTGACCCC TGTAAAAGGA GAGCCCCTGG GTGTTGTGTG CAACTGGCCC 
CCGTCGGTAG AAGCCGCACT GCAGCGCTGG GGAACAACAC AAGCAAAATG TTCATGTCTT 
ACCGCTCTTG ATGTCACTGG CAAACCAACC TATACGTTAA CATATGGCAA GCTATGGACC 
AGGAGTGTCA GACTGGCTTA TACCCTGCTG AACAAATTGG GTACCAAGAA TGAACAAGTG 
CTGAAGCCTG GGGACAGGGT TGCCCTGGTT TACCCCAATA ATGACCCAGT GATGTTCATG 
GTTGCGTTTT ATGGCTGTCT CTTGGCGGAG GTGATCCCAG TGCCGATTGA GGTACCTCTT 
ACTAGAAAGG ACGCTGGAGG GCATCAGATT GGATTCCTGC TGGGAAGCTG TGGTGTTAAG 
ATCGCTCTTA CTACTGAGGT TTGCTTGAAG GGGCTGCCCA AGACACAGAA CGGGGAGATT 
GTGCAATTCA AAGGTTGGCC AAAACTCAAG TGGGTTGTAA CAGACTCCAG ATACCTATCA 
AAATCACCAA AGGACTGGCA GCCACACATC ACCAATGCTG GCACAGAGCC GGCATACATT 
GAGTACAAAA CAAGCAAGGA AGGCAGTGTG ATGGGTGTGA CCGTGTCTCG GATAGCCATG 
CTGTCTCATT GCCAGGCACT CTCACAGGCC TGTAACTACA CAGAGGGTGA GACCTTAGTT 
AATGTCCTGG ACTTCAAGAG GGATGCAGGC CTGTGGCATG GTATACTAAC GAATGTAGTA 
AATAAAATGC ACACGATCAG CGTGCCGTAC GCAGTGATGA AAGCATGTCC GTTGTCCTGG 
GTGCAGAGGG TACATGCCAG TAAAGGCAGG GTGGCTGTAG TGAAGTGCCG GGACTTGCAC 
TGGGCCATGA TAGCCCACCG AGAACAAAAG GATATCAGTC TGGCCTCTCT CCGTATGCTC 
ATTGTTACAG ATGGAGCTAA TCCTTGGTCC GTTTCCTCAT GTGATGCATT CCTGAGTTTG 
TTTCAGAATC ACGGCCTGAA GCCTGAGGTC ATCTGTCCAT GTGCAACCTC TCCAGAAGCT 
ATGACTGTCG CTATTCGAAG GCCTGGAGTA CCCGGTGCCC CCCTGCCTGG AAGAGCCATT 
TTGTCTATGA ATGGGTTAAG TTATGGCGTG ATCAGAGTTA ATACAGAGGA CAAGAATTCT 
GCATTGACCG TCCAGGACGT TGGACATGTT ATGCCAGGAA GTGTGATGTG CACTGTGAAG 
CCGGAAGGAG TACCCCAGCT CTGTAAAACT GATGAGATAG GAGAGATCTG TGTGAGCTGT 
AAAGGAGGAG GCACAATGTA CTATGGCCTG GCAGGGCTCA CCAAGAACAC CTTTGAGGTT 
TTGCCAGTTA ATGCTTCTGG GGCTTCTATA GGAGAAATAC CCTTCATCAG AACTGGGCTA 
CTTGGATTTG TGGGGCCAGG CAGTTTAGTG TTTGTGGTGG GAAAGATGGA CGGCACGGTG 
ATGGTGAGTG GGAGGCGCCA CAATGCTGAT GATATTGTGG CTACTGCCCT GGCGGTGGAA 
TCCTTTAAAA CCCTGTACAG AGGAAGGATT GCTGTGTTTT CAGTCACAGT ATTTTATGAC 
GAGCGGATTG TGGTGGTGGC AGAACAGCGC CCTGATGCTT CTGAGGAAGA TTCCTTCCAA 
TGGATGAGCC GTGTATTACA GGCCATTGAT AGTATCCACC AAGTGGGAGT TTACTGTCTT 
GCCCTGGTCC CAGCCAATAC GTTGCCAAAA ACTCCACTGG GAGGAATCCA CATATCGCAA 
ACTAAACAGC TTTTCTTGGA GGGCAGCTTA CACCCCTGTA ACATACTCAT GTGTCCTCAT 
ACGTGTGTAA CCAACTTGCC AAAACCAAGG CAGAAGCAGC CAGGAGTTGG GCCAGCATCT 
GTCATGGTGG GTAATCTGGT CGCAGGAAAA AGAATTGCTC AGGCCTCCGG AAGAGATTTG 
GGACAAATAG AGGAAAATGA TTTGGTGAAA AAGCACCAGT ATCTGACGGA AGTCTTACAG 
TGGCGAGCGC AAACCACTCC CGAACACGTT CTTTTTATCC TCCTCAATGC AAAGGGAACG 
GCAGTTTGCA ATGCCTCATG TTTACAGCTA CACAAGAGAG CAGAAAGAAT AGCAGCTGCC 
CTATGTGATA AAGGTCATCT CAACGCCGGC GATAACGTGG TGCTACTTTT TCCTCCCGGA 
ATTGAGTTGA TAGCTGCATT CTATGGCTGC CTGTACGCTG GCTGTATTCC TGTAACTGTC 
CGGCCGCCTC ACGCACAGAA CCTCGCTGCC ACCTTACCTA CAGTACGAAT GATTGTAGAT 
GTTAGCAAAG CTGCCTGCAT TCTCACTACA CAAGCATTGC TGAGATTGCT CAAGTCCAAA 
GAAGCAGCAG CGGCTGTGGA TGTGAAAACT TGGCCAACCA TCATCGACAC CGATGATTTG 
CCCAGGAAAC GGGTACCGCA GCTGTATAAA CCACCTACAC CTGAAATGCT GGCTTACCTG 
GACTTCAGTG TGAGCACGAC TGGCATGCTT ACGGGGGTTA AGATGTCTCA TGGATCAGTA 
AATGCACTGT GCCGGTCCAT AAAGCTGCAG TGTGAGCTGT ACTCTACTCG CCAGATCGCC 
ATTTGTCTTG ATCCTTATTG TGGGCTTGGC TTTGTACTTT GGTGCCTTTG TAGTGTTTAT 
TCTGGTCATC AGTCTATATT AATTCCTCCT ATGGAGTTGG AATCCAACTT ATTCTTGTGG 
CTCTCTGCTG TGAGTCAGTA TAAAATTAGG GACACGTTCT GTTCATATTC TGTGATGGAG 
ATGTGCTCAA AGGGACTGGG GAACCAGATT GAGGTGCTAA AGACCAAAGG CATTAATCTG 
TCTTACGTGC GGACCTGTGT GGTGGTTGCT GAAGAGCGGC CGAGGGTGGC TTTGGCATAT 
TCCTTTTCAA AACTCTTCAA AGACCTTGGG CTGTCCCCCC GCGCTGTGAG CACAACCTTT 
GGATCCAGAG TTAACACCGC TATATGCTTG CAGGGAACCT CAGGGCCAGA TCCTACAACT 
GTGTATGTGG ATCTGAAATC CCTTCGGCAT GACAGGGTGC GCCTGGTGGA GAGGGGATCT 
CCGCAGAGTT TGCTCCTATT GGAATCTGGA AAGATTTTAC CTGGTGTCAC CGTAATTATT 
GTTAATCCAG AGACCAAAGG ACCATTGGGG GATTCCCACC TTGGAGAGAT CTGGGTGAAC 
AGCGCCCACA CAGCCAGCGG ATACTACACA ATATATGATA GTGAGACCTT ACAGGCTGAT 
CATTTCAATA CCAGGCTTAG CTTTGGAGAC ACACAGACAC TTTGGGCCAA AACGGGCTAT 
CTTGGCTTTG TCCGCAGGAC AGAACTGACT GATGCAAATG GAGAGCGGCA CGATGCGCTG 
TTTGTGGTTG GAGCACTAGA TGAAACGCTG GAGCTGAGGG GGCTTCGTTA TCATCCGATA 
GATATCGAGA CCTCAGTGTC ACGCATACAC CGGAGTATCG CTGAATGTGC TGTTTTCACA 
TGGACCAACC TGCTGGTAGT GGTGGTGGAG CTGTGCGGCT CAGAACAGGA GGCTCTGGAC 
TTGGTTCCTC TGGTGACCAA CGTGGTGTTG GAAGAACATT ACCTGATTGT GGGCGTGGTG 
GTGGTGGTGG ATCCCGGCGT GATACCCATC AATTCCCGGG GAGAGAAACA ACGGATGCAC 
CTGCGGGACA GTTTCTTAGC GGACCAGTTG GATCCAATCT ACGTGGCGTA CAACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933754.2
CDS	187..4926
Translation	MAERAFDLSALPKEVREQLAELELELSEGDITQKGYEKKRAKLLGPYIPQTQGVDPALQKENRNQTTGSPAAPAQSSSKYHRARSGGARDERYRSDIHTEAVQAALAKHKEQKMALPMPTKRRSTFVQSPADACTPPDTSSASEDEGSLRRQAALSAALAQSLQNSEAWINRSVQGSSTSSSASSTLSHGEVKSTSGTLADILSNTHIDSSPAPPDVTNTTNSTTTTSTSSSSSSLSSVRPATIDLPPAGLVKGVHKAARRSSLMDTADGVAVNSRVSTKIQQLLNTLKRPKRPPLKEFFVDDFEEIVEVPRPDPNQPKPEGRQVTPVKGEPLGVVCNWPPSVEAALQRWGTTQAKCSCLTALDVTGKPTYTLTYGKLWTRSVRLAYTLLNKLGTKNEQVLKPGDRVALVYPNNDPVMFMVAFYGCLLAEVIPVPIEVPLTRKDAGGHQIGFLLGSCGVKIALTTEVCLKGLPKTQNGEIVQFKGWPKLKWVVTDSRYLSKSPKDWQPHITNAGTEPAYIEYKTSKEGSVMGVTVSRIAMLSHCQALSQACNYTEGETLVNVLDFKRDAGLWHGILTNVVNKMHTISVPYAVMKACPLSWVQRVHASKGRVAVVKCRDLHWAMIAHREQKDISLASLRMLIVTDGANPWSVSSCDAFLSLFQNHGLKPEVICPCATSPEAMTVAIRRPGVPGAPLPGRAILSMNGLSYGVIRVNTEDKNSALTVQDVGHVMPGSVMCTVKPEGVPQLCKTDEIGEICVSCKGGGTMYYGLAGLTKNTFEVLPVNASGASIGEIPFIRTGLLGFVGPGSLVFVVGKMDGTVMVSGRRHNADDIVATALAVESFKTLYRGRIAVFSVTVFYDERIVVVAEQRPDASEEDSFQWMSRVLQAIDSIHQVGVYCLALVPANTLPKTPLGGIHISQTKQLFLEGSLHPCNILMCPHTCVTNLPKPRQKQPGVGPASVMVGNLVAGKRIAQASGRDLGQIEENDLVKKHQYLTEVLQWRAQTTPEHVLFILLNAKGTAVCNASCLQLHKRAERIAAALCDKGHLNAGDNVVLLFPPGIELIAAFYGCLYAGCIPVTVRPPHAQNLAATLPTVRMIVDVSKAACILTTQALLRLLKSKEAAAAVDVKTWPTIIDTDDLPRKRVPQLYKPPTPEMLAYLDFSVSTTGMLTGVKMSHGSVNALCRSIKLQCELYSTRQIAICLDPYCGLGFVLWCLCSVYSGHQSILIPPMELESNLFLWLSAVSQYKIRDTFCSYSVMEMCSKGLGNQIEVLKTKGINLSYVRTCVVVAEERPRVALAYSFSKLFKDLGLSPRAVSTTFGSRVNTAICLQGTSGPDPTTVYVDLKSLRHDRVRLVERGSPQSLLLLESGKILPGVTVIIVNPETKGPLGDSHLGEIWVNSAHTASGYYTIYDSETLQADHFNTRLSFGDTQTLWAKTGYLGFVRRTELTDANGERHDALFVVGALDETLELRGLRYHPIDIETSVSRIHRSIAECAVFTWTNLLVVVVELCGSEQEALDLVPLVTNVVLEEHYLIVGVVVVVDPGVIPINSRGEKQRMHLRDSFLADQLDPIYVAYNM

Target ORF information:

RefSeq Version	XM_002933708.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933708.5

ORF Insert Sequence:

ATGGCGGAGA GAGCCTTCGA CCTATCCGCC CTCCCGAAGG AGGTGAGGGA GCAGCTGGCA 
GAACTGGAGC TGGAGCTGTC AGAAGGTGAT ATCACCCAGA AGGGTTATGA GAAAAAAAGA 
GCAAAACTAT TGGGTCCGTA CATCCCGCAA ACACAAGGTG TTGATCCAGC GCTACAGAAA 
GAGAATCGGA ACCAAACAAC TGGATCACCT GCTGCTCCTG CGCAATCGTC CTCTAAGTAC 
CATCGAGCCA GATCCGGAGG AGCTAGAGAT GAGCGTTACA GATCAGATAT CCATACAGAA 
GCAGTCCAAG CGGCTCTGGC TAAGCATAAA GAACAGAAGA TGGCATTACC AATGCCAACC 
AAAAGGCGGT CAACATTTGT GCAGTCTCCA GCTGACGCAT GTACCCCTCC AGATACATCC 
TCCGCATCTG AGGACGAAGG ATCCCTAAGA CGCCAGGCTG CTCTTTCGGC AGCTCTTGCT 
CAGAGTTTAC AGAATTCAGA GGCCTGGATC AACAGGTCCG TTCAGGGTTC ATCCACATCA 
TCTTCAGCAT CCTCCACATT ATCGCACGGA GAAGTAAAGA GTACCAGTGG CACCTTAGCA 
GATATACTTT CTAATACTCA CATAGACAGT TCTCCTGCTC CACCCGATGT TACCAACACC 
ACCAATTCCA CAACCACTAC AAGCACATCA TCGTCTTCGT CATCCTTGTC TTCAGTTCGC 
CCAGCAACCA TTGACCTTCC CCCTGCAGGC CTGGTGAAAG GGGTGCACAA GGCAGCTAGA 
AGGTCTAGCT TAATGGATAC TGCAGATGGT GTTGCAGTGA ACAGTCGCGT CTCAACAAAA 
ATCCAGCAGT TGCTGAACAC GCTCAAGCGA CCAAAAAGGC CACCATTAAA GGAATTTTTT 
GTGGATGATT TTGAAGAGAT TGTAGAAGTA CCCCGACCAG ACCCAAACCA GCCCAAACCA 
GAAGGTCGCC AGGTGACCCC TGTAAAAGGA GAGCCCCTGG GTGTTGTGTG CAACTGGCCC 
CCGTCGGTAG AAGCCGCACT GCAGCGCTGG GGAACAACAC AAGCAAAATG TTCATGTCTT 
ACCGCTCTTG ATGTCACTGG CAAACCAACC TATACGTTAA CATATGGCAA GCTATGGACC 
AGGAGTGTCA GACTGGCTTA TACCCTGCTG AACAAATTGG GTACCAAGAA TGAACAAGTG 
CTGAAGCCTG GGGACAGGGT TGCCCTGGTT TACCCCAATA ATGACCCAGT GATGTTCATG 
GTTGCGTTTT ATGGCTGTCT CTTGGCGGAG GTGATCCCAG TGCCGATTGA GGTACCTCTT 
ACTAGAAAGG ACGCTGGAGG GCATCAGATT GGATTCCTGC TGGGAAGCTG TGGTGTTAAG 
ATCGCTCTTA CTACTGAGGT TTGCTTGAAG GGGCTGCCCA AGACACAGAA CGGGGAGATT 
GTGCAATTCA AAGGTTGGCC AAAACTCAAG TGGGTTGTAA CAGACTCCAG ATACCTATCA 
AAATCACCAA AGGACTGGCA GCCACACATC ACCAATGCTG GCACAGAGCC GGCATACATT 
GAGTACAAAA CAAGCAAGGA AGGCAGTGTG ATGGGTGTGA CCGTGTCTCG GATAGCCATG 
CTGTCTCATT GCCAGGCACT CTCACAGGCC TGTAACTACA CAGAGGGTGA GACCTTAGTT 
AATGTCCTGG ACTTCAAGAG GGATGCAGGC CTGTGGCATG GTATACTAAC GAATGTAGTA 
AATAAAATGC ACACGATCAG CGTGCCGTAC GCAGTGATGA AAGCATGTCC GTTGTCCTGG 
GTGCAGAGGG TACATGCCAG TAAAGGCAGG GTGGCTGTAG TGAAGTGCCG GGACTTGCAC 
TGGGCCATGA TAGCCCACCG AGAACAAAAG GATATCAGTC TGGCCTCTCT CCGTATGCTC 
ATTGTTACAG ATGGAGCTAA TCCTTGGTCC GTTTCCTCAT GTGATGCATT CCTGAGTTTG 
TTTCAGAATC ACGGCCTGAA GCCTGAGGTC ATCTGTCCAT GTGCAACCTC TCCAGAAGCT 
ATGACTGTCG CTATTCGAAG GCCTGGAGTA CCCGGTGCCC CCCTGCCTGG AAGAGCCATT 
TTGTCTATGA ATGGGTTAAG TTATGGCGTG ATCAGAGTTA ATACAGAGGA CAAGAATTCT 
GCATTGACCG TCCAGGACGT TGGACATGTT ATGCCAGGAA GTGTGATGTG CACTGTGAAG 
CCGGAAGGAG TACCCCAGCT CTGTAAAACT GATGAGATAG GAGAGATCTG TGTGAGCTGT 
AAAGGAGGAG GCACAATGTA CTATGGCCTG GCAGGGCTCA CCAAGAACAC CTTTGAGGTT 
TTGCCAGTTA ATGCTTCTGG GGCTTCTATA GGAGAAATAC CCTTCATCAG AACTGGGCTA 
CTTGGATTTG TGGGGCCAGG CAGTTTAGTG TTTGTGGTGG GAAAGATGGA CGGCACGGTG 
ATGGTGAGTG GGAGGCGCCA CAATGCTGAT GATATTGTGG CTACTGCCCT GGCGGTGGAA 
TCCTTTAAAA CCCTGTACAG AGGAAGGATT GCTGTGTTTT CAGTCACAGT ATTTTATGAC 
GAGCGGATTG TGGTGGTGGC AGAACAGCGC CCTGATGCTT CTGAGGAAGA TTCCTTCCAA 
TGGATGAGCC GTGTATTACA GGCCATTGAT AGTATCCACC AAGTGGGAGT TTACTGTCTT 
GCCCTGGTCC CAGCCAATAC GTTGCCAAAA ACTCCACTGG GAGGAATCCA CATATCGCAA 
ACTAAACAGC TTTTCTTGGA GGGCAGCTTA CACCCCTGTA ACATACTCAT GTGTCCTCAT 
ACGTGTGTAA CCAACTTGCC AAAACCAAGG CAGAAGCAGC CAGGAGTTGG GCCAGCATCT 
GTCATGGTGG GTAATCTGGT CGCAGGAAAA AGAATTGCTC AGGCCTCCGG AAGAGATTTG 
GGACAAATAG AGGAAAATGA TTTGGTGAAA AAGCACCAGT ATCTGACGGA AGTCTTACAG 
TGGCGAGCGC AAACCACTCC CGAACACGTT CTTTTTATCC TCCTCAATGC AAAGGGAACG 
GCAGTTTGCA ATGCCTCATG TTTACAGCTA CACAAGAGAG CAGAAAGAAT AGCAGCTGCC 
CTATGTGATA AAGGTCATCT CAACGCCGGC GATAACGTGG TGCTACTTTT TCCTCCCGGA 
ATTGAGTTGA TAGCTGCATT CTATGGCTGC CTGTACGCTG GCTGTATTCC TGTAACTGTC 
CGGCCGCCTC ACGCACAGAA CCTCGCTGCC ACCTTACCTA CAGTACGAAT GATTGTAGAT 
GTTAGCAAAG CTGCCTGCAT TCTCACTACA CAAGCATTGC TGAGATTGCT CAAGTCCAAA 
GAAGCAGCAG CGGCTGTGGA TGTGAAAACT TGGCCAACCA TCATCGACAC CGATGATTTG 
CCCAGGAAAC GGGTACCGCA GCTGTATAAA CCACCTACAC CTGAAATGCT GGCTTACCTG 
GACTTCAGTG TGAGCACGAC TGGCATGCTT ACGGGGGTTA AGATGTCTCA TGGATCAGTA 
AATGCACTGT GCCGGTCCAT AAAGCTGCAG TGTGAGCTGT ACTCTACTCG CCAGATCGCC 
ATTTGTCTTG ATCCTTATTG TGGGCTTGGC TTTGTACTTT GGTGCCTTTG TAGTGTTTAT 
TCTGGTCATC AGTCTATATT AATTCCTCCT ATGGAGTTGG AATCCAACTT ATTCTTGTGG 
CTCTCTGCTG TGAGTCAGTA TAAAATTAGG GACACGTTCT GTTCATATTC TGTGATGGAG 
ATGTGCTCAA AGGGACTGGG GAACCAGATT GAGGTGCTAA AGACCAAAGG CATTAATCTG 
TCTTACGTGC GGACCTGTGT GGTGGTTGCT GAAGAGCGGC CGAGGGTGGC TTTGGCATAT 
TCCTTTTCAA AACTCTTCAA AGACCTTGGG CTGTCCCCCC GCGCTGTGAG CACAACCTTT 
GGATCCAGAG TTAACACCGC TATATGCTTG CAGGGAACCT CAGGGCCAGA TCCTACAACT 
GTGTATGTGG ATCTGAAATC CCTTCGGCAT GACAGGGTGC GCCTGGTGGA GAGGGGATCT 
CCGCAGAGTT TGCTCCTATT GGAATCTGGA AAGATTTTAC CTGGTGTCAC CGTAATTATT 
GTTAATCCAG AGACCAAAGG ACCATTGGGG GATTCCCACC TTGGAGAGAT CTGGGTGAAC 
AGCGCCCACA CAGCCAGCGG ATACTACACA ATATATGATA GTGAGACCTT ACAGGCTGAT 
CATTTCAATA CCAGGCTTAG CTTTGGAGAC ACACAGACAC TTTGGGCCAA AACGGGCTAT 
CTTGGCTTTG TCCGCAGGAC AGAACTGACT GATGCAAATG GAGAGCGGCA CGATGCGCTG 
TTTGTGGTTG GAGCACTAGA TGAAACGCTG GAGCTGAGGG GGCTTCGTTA TCATCCGATA 
GATATCGAGA CCTCAGTGTC ACGCATACAC CGGAGTATCG CTGAATGTGC TGTTTTCACA 
TGGACCAACC TGCTGGTAGT GGTGGTGGAG CTGTGCGGCT CAGAACAGGA GGCTCTGGAC 
TTGGTTCCTC TGGTGACCAA CGTGGTGTTG GAAGAACATT ACCTGATTGT GGGCGTGGTG 
GTGGTGGTGG ATCCCGGCGT GATACCCATC AATTCCCGGG GAGAGAAACA ACGGATGCAC 
CTGCGGGACA GTTTCTTAGC GGACCAGTTG GATCCAATCT ACGTGGCGTA CAACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dip2b ( XM_004911909.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911966.1 Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X2, mRNA.

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CloneID	OXa52713
Clone ID Related Accession (Same CDS sequence)	XM_004911909.4
Accession Version	XM_004911909.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	disco-interacting protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911909.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGA GAGCCTTCGA CCTATCCGCC CTCCCGAAGG AGGTGAGGGA GCAGCTGGCA 
GAACTGGAGC TGGAGCTGTC AGAAGGTGAT ATCACCCAGA AGGGTTATGA GAAAAAAAGA 
GCAAAACTAT TGGGTCCGTA CATCCCGCAA ACACAAGGTG TTGATCCAGC GCTACAGAAA 
GAGAATCGGA ACCAAACAAC TGGATCACCT GCTGCTCCTG CGCAATCGTC CTCTAAGTAC 
CATCGAGCCA GATCCGGAGG AGCTAGAGAT GAGCGTTACA GATCAGATAT CCATACAGAA 
GCAGTCCAAG CGGCTCTGGC TAAGCATAAA GAACAGAAGA TGGCATTACC AATGCCAACC 
AAAAGGCGGT CAACATTTGT GCAGTCTCCA GCTGACGCAT GTACCCCTCC AGATACATCC 
TCCGCATCTG AGGACGAAGG ATCCCTAAGA CGCCAGGCTG CTCTTTCGGC AGCTCTTGCT 
CAGAGTTTAC AGAATTCAGA GGCCTGGATC AACAGGTCCG TTCAGGGTTC ATCCACATCA 
TCTTCAGCAT CCTCCACATT ATCGCACGGA GAAGTAAAGA GTACCAGTGG CACCTTAGCA 
GATATACTTT CTAATACTCA CATAGACAGT TCTCCTGCTC CACCCGATGT TACCAACACC 
ACCAATTCCA CAACCACTAC AAGCACATCA TCGTCTTCGT CATCCTTGTC TTCAGTTCGC 
CCAGCAACCA TTGACCTTCC CCCTGCAGGC CTGGTGAAAG GGGTGCACAA GGCAGCTAGA 
AGGTCTAGCT TAATGGATAC TGCAGATGGT GTTGCAGTGA ACAGTCGCGT CTCAACAAAA 
ATCCAGCAGT TGCTGAACAC GCTCAAGCGA CCAAAAAGGC CACCATTAAA GGAATTTTTT 
GTGGATGATT TTGAAGAGAT TGTAGAAGTA CCCCGACCAG ACCCAAACCA GCCCAAACCA 
GAAGGTCGCC AGGTGACCCC TGTAAAAGGA GAGCCCCTGG GTGTTGTGTG CAACTGGCCC 
CCGTCGGTAG AAGCCGCACT GCAGCGCTGG GGAACAACAC AAGCAAAATG TTCATGTCTT 
ACCGCTCTTG ATGTCACTGG CAAACCAACC TATACGTTAA CATATGGCAA GCTATGGACC 
AGGAGTGTCA GACTGGCTTA TACCCTGCTG AACAAATTGG GTACCAAGAA TGAACAAGTG 
CTGAAGCCTG GGGACAGGGT TGCCCTGGTT TACCCCAATA ATGACCCAGT GATGTTCATG 
GTTGCGTTTT ATGGCTGTCT CTTGGCGGAG GTGATCCCAG TGCCGATTGA GGACGCTGGA 
GGGCATCAGA TTGGATTCCT GCTGGGAAGC TGTGGTGTTA AGATCGCTCT TACTACTGAG 
GTTTGCTTGA AGGGGCTGCC CAAGACACAG AACGGGGAGA TTGTGCAATT CAAAGGTTGG 
CCAAAACTCA AGTGGGTTGT AACAGACTCC AGATACCTAT CAAAATCACC AAAGGACTGG 
CAGCCACACA TCACCAATGC TGGCACAGAG CCGGCATACA TTGAGTACAA AACAAGCAAG 
GAAGGCAGTG TGATGGGTGT GACCGTGTCT CGGATAGCCA TGCTGTCTCA TTGCCAGGCA 
CTCTCACAGG CCTGTAACTA CACAGAGGGT GAGACCTTAG TTAATGTCCT GGACTTCAAG 
AGGGATGCAG GCCTGTGGCA TGGTATACTA ACGAATGTAG TAAATAAAAT GCACACGATC 
AGCGTGCCGT ACGCAGTGAT GAAAGCATGT CCGTTGTCCT GGGTGCAGAG GGTACATGCC 
AGTAAAGGCA GGGTGGCTGT AGTGAAGTGC CGGGACTTGC ACTGGGCCAT GATAGCCCAC 
CGAGAACAAA AGGATATCAG TCTGGCCTCT CTCCGTATGC TCATTGTTAC AGATGGAGCT 
AATCCTTGGT CCGTTTCCTC ATGTGATGCA TTCCTGAGTT TGTTTCAGAA TCACGGCCTG 
AAGCCTGAGG TCATCTGTCC ATGTGCAACC TCTCCAGAAG CTATGACTGT CGCTATTCGA 
AGGCCTGGAG TACCCGGTGC CCCCCTGCCT GGAAGAGCCA TTTTGTCTAT GAATGGGTTA 
AGTTATGGCG TGATCAGAGT TAATACAGAG GACAAGAATT CTGCATTGAC CGTCCAGGAC 
GTTGGACATG TTATGCCAGG AAGTGTGATG TGCACTGTGA AGCCGGAAGG AGTACCCCAG 
CTCTGTAAAA CTGATGAGAT AGGAGAGATC TGTGTGAGCT GTAAAGGAGG AGGCACAATG 
TACTATGGCC TGGCAGGGCT CACCAAGAAC ACCTTTGAGG TTTTGCCAGT TAATGCTTCT 
GGGGCTTCTA TAGGAGAAAT ACCCTTCATC AGAACTGGGC TACTTGGATT TGTGGGGCCA 
GGCAGTTTAG TGTTTGTGGT GGGAAAGATG GACGGCACGG TGATGGTGAG TGGGAGGCGC 
CACAATGCTG ATGATATTGT GGCTACTGCC CTGGCGGTGG AATCCTTTAA AACCCTGTAC 
AGAGGAAGGA TTGCTGTGTT TTCAGTCACA GTATTTTATG ACGAGCGGAT TGTGGTGGTG 
GCAGAACAGC GCCCTGATGC TTCTGAGGAA GATTCCTTCC AATGGATGAG CCGTGTATTA 
CAGGCCATTG ATAGTATCCA CCAAGTGGGA GTTTACTGTC TTGCCCTGGT CCCAGCCAAT 
ACGTTGCCAA AAACTCCACT GGGAGGAATC CACATATCGC AAACTAAACA GCTTTTCTTG 
GAGGGCAGCT TACACCCCTG TAACATACTC ATGTGTCCTC ATACGTGTGT AACCAACTTG 
CCAAAACCAA GGCAGAAGCA GCCAGGAGTT GGGCCAGCAT CTGTCATGGT GGGTAATCTG 
GTCGCAGGAA AAAGAATTGC TCAGGCCTCC GGAAGAGATT TGGGACAAAT AGAGGAAAAT 
GATTTGGTGA AAAAGCACCA GTATCTGACG GAAGTCTTAC AGTGGCGAGC GCAAACCACT 
CCCGAACACG TTCTTTTTAT CCTCCTCAAT GCAAAGGGAA CGGCAGTTTG CAATGCCTCA 
TGTTTACAGC TACACAAGAG AGCAGAAAGA ATAGCAGCTG CCCTATGTGA TAAAGGTCAT 
CTCAACGCCG GCGATAACGT GGTGCTACTT TTTCCTCCCG GAATTGAGTT GATAGCTGCA 
TTCTATGGCT GCCTGTACGC TGGCTGTATT CCTGTAACTG TCCGGCCGCC TCACGCACAG 
AACCTCGCTG CCACCTTACC TACAGTACGA ATGATTGTAG ATGTTAGCAA AGCTGCCTGC 
ATTCTCACTA CACAAGCATT GCTGAGATTG CTCAAGTCCA AAGAAGCAGC AGCGGCTGTG 
GATGTGAAAA CTTGGCCAAC CATCATCGAC ACCGATGATT TGCCCAGGAA ACGGGTACCG 
CAGCTGTATA AACCACCTAC ACCTGAAATG CTGGCTTACC TGGACTTCAG TGTGAGCACG 
ACTGGCATGC TTACGGGGGT TAAGATGTCT CATGGATCAG TAAATGCACT GTGCCGGTCC 
ATAAAGCTGC AGTGTGAGCT GTACTCTACT CGCCAGATCG CCATTTGTCT TGATCCTTAT 
TGTGGGCTTG GCTTTGTACT TTGGTGCCTT TGTAGTGTTT ATTCTGGTCA TCAGTCTATA 
TTAATTCCTC CTATGGAGTT GGAATCCAAC TTATTCTTGT GGCTCTCTGC TGTGAGTCAG 
TATAAAATTA GGGACACGTT CTGTTCATAT TCTGTGATGG AGATGTGCTC AAAGGGACTG 
GGGAACCAGA TTGAGGTGCT AAAGACCAAA GGCATTAATC TGTCTTACGT GCGGACCTGT 
GTGGTGGTTG CTGAAGAGCG GCCGAGGGTG GCTTTGGCAT ATTCCTTTTC AAAACTCTTC 
AAAGACCTTG GGCTGTCCCC CCGCGCTGTG AGCACAACCT TTGGATCCAG AGTTAACACC 
GCTATATGCT TGCAGGGAAC CTCAGGGCCA GATCCTACAA CTGTGTATGT GGATCTGAAA 
TCCCTTCGGC ATGACAGGGT GCGCCTGGTG GAGAGGGGAT CTCCGCAGAG TTTGCTCCTA 
TTGGAATCTG GAAAGATTTT ACCTGGTGTC ACCGTAATTA TTGTTAATCC AGAGACCAAA 
GGACCATTGG GGGATTCCCA CCTTGGAGAG ATCTGGGTGA ACAGCGCCCA CACAGCCAGC 
GGATACTACA CAATATATGA TAGTGAGACC TTACAGGCTG ATCATTTCAA TACCAGGCTT 
AGCTTTGGAG ACACACAGAC ACTTTGGGCC AAAACGGGCT ATCTTGGCTT TGTCCGCAGG 
ACAGAACTGA CTGATGCAAA TGGAGAGCGG CACGATGCGC TGTTTGTGGT TGGAGCACTA 
GATGAAACGC TGGAGCTGAG GGGGCTTCGT TATCATCCGA TAGATATCGA GACCTCAGTG 
TCACGCATAC ACCGGAGTAT CGCTGAATGT GCTGTTTTCA CATGGACCAA CCTGCTGGTA 
GTGGTGGTGG AGCTGTGCGG CTCAGAACAG GAGGCTCTGG ACTTGGTTCC TCTGGTGACC 
AACGTGGTGT TGGAAGAACA TTACCTGATT GTGGGCGTGG TGGTGGTGGT GGATCCCGGC 
GTGATACCCA TCAATTCCCG GGGAGAGAAA CAACGGATGC ACCTGCGGGA CAGTTTCTTA 
GCGGACCAGT TGGATCCAAT CTACGTGGCG TACAACATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911966.1
CDS	188..4909
Translation	MAERAFDLSALPKEVREQLAELELELSEGDITQKGYEKKRAKLLGPYIPQTQGVDPALQKENRNQTTGSPAAPAQSSSKYHRARSGGARDERYRSDIHTEAVQAALAKHKEQKMALPMPTKRRSTFVQSPADACTPPDTSSASEDEGSLRRQAALSAALAQSLQNSEAWINRSVQGSSTSSSASSTLSHGEVKSTSGTLADILSNTHIDSSPAPPDVTNTTNSTTTTSTSSSSSSLSSVRPATIDLPPAGLVKGVHKAARRSSLMDTADGVAVNSRVSTKIQQLLNTLKRPKRPPLKEFFVDDFEEIVEVPRPDPNQPKPEGRQVTPVKGEPLGVVCNWPPSVEAALQRWGTTQAKCSCLTALDVTGKPTYTLTYGKLWTRSVRLAYTLLNKLGTKNEQVLKPGDRVALVYPNNDPVMFMVAFYGCLLAEVIPVPIEDAGGHQIGFLLGSCGVKIALTTEVCLKGLPKTQNGEIVQFKGWPKLKWVVTDSRYLSKSPKDWQPHITNAGTEPAYIEYKTSKEGSVMGVTVSRIAMLSHCQALSQACNYTEGETLVNVLDFKRDAGLWHGILTNVVNKMHTISVPYAVMKACPLSWVQRVHASKGRVAVVKCRDLHWAMIAHREQKDISLASLRMLIVTDGANPWSVSSCDAFLSLFQNHGLKPEVICPCATSPEAMTVAIRRPGVPGAPLPGRAILSMNGLSYGVIRVNTEDKNSALTVQDVGHVMPGSVMCTVKPEGVPQLCKTDEIGEICVSCKGGGTMYYGLAGLTKNTFEVLPVNASGASIGEIPFIRTGLLGFVGPGSLVFVVGKMDGTVMVSGRRHNADDIVATALAVESFKTLYRGRIAVFSVTVFYDERIVVVAEQRPDASEEDSFQWMSRVLQAIDSIHQVGVYCLALVPANTLPKTPLGGIHISQTKQLFLEGSLHPCNILMCPHTCVTNLPKPRQKQPGVGPASVMVGNLVAGKRIAQASGRDLGQIEENDLVKKHQYLTEVLQWRAQTTPEHVLFILLNAKGTAVCNASCLQLHKRAERIAAALCDKGHLNAGDNVVLLFPPGIELIAAFYGCLYAGCIPVTVRPPHAQNLAATLPTVRMIVDVSKAACILTTQALLRLLKSKEAAAAVDVKTWPTIIDTDDLPRKRVPQLYKPPTPEMLAYLDFSVSTTGMLTGVKMSHGSVNALCRSIKLQCELYSTRQIAICLDPYCGLGFVLWCLCSVYSGHQSILIPPMELESNLFLWLSAVSQYKIRDTFCSYSVMEMCSKGLGNQIEVLKTKGINLSYVRTCVVVAEERPRVALAYSFSKLFKDLGLSPRAVSTTFGSRVNTAICLQGTSGPDPTTVYVDLKSLRHDRVRLVERGSPQSLLLLESGKILPGVTVIIVNPETKGPLGDSHLGEIWVNSAHTASGYYTIYDSETLQADHFNTRLSFGDTQTLWAKTGYLGFVRRTELTDANGERHDALFVVGALDETLELRGLRYHPIDIETSVSRIHRSIAECAVFTWTNLLVVVVELCGSEQEALDLVPLVTNVVLEEHYLIVGVVVVVDPGVIPINSRGEKQRMHLRDSFLADQLDPIYVAYNM

Target ORF information:

RefSeq Version	XM_004911909.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911909.4

ORF Insert Sequence:

ATGGCGGAGA GAGCCTTCGA CCTATCCGCC CTCCCGAAGG AGGTGAGGGA GCAGCTGGCA 
GAACTGGAGC TGGAGCTGTC AGAAGGTGAT ATCACCCAGA AGGGTTATGA GAAAAAAAGA 
GCAAAACTAT TGGGTCCGTA CATCCCGCAA ACACAAGGTG TTGATCCAGC GCTACAGAAA 
GAGAATCGGA ACCAAACAAC TGGATCACCT GCTGCTCCTG CGCAATCGTC CTCTAAGTAC 
CATCGAGCCA GATCCGGAGG AGCTAGAGAT GAGCGTTACA GATCAGATAT CCATACAGAA 
GCAGTCCAAG CGGCTCTGGC TAAGCATAAA GAACAGAAGA TGGCATTACC AATGCCAACC 
AAAAGGCGGT CAACATTTGT GCAGTCTCCA GCTGACGCAT GTACCCCTCC AGATACATCC 
TCCGCATCTG AGGACGAAGG ATCCCTAAGA CGCCAGGCTG CTCTTTCGGC AGCTCTTGCT 
CAGAGTTTAC AGAATTCAGA GGCCTGGATC AACAGGTCCG TTCAGGGTTC ATCCACATCA 
TCTTCAGCAT CCTCCACATT ATCGCACGGA GAAGTAAAGA GTACCAGTGG CACCTTAGCA 
GATATACTTT CTAATACTCA CATAGACAGT TCTCCTGCTC CACCCGATGT TACCAACACC 
ACCAATTCCA CAACCACTAC AAGCACATCA TCGTCTTCGT CATCCTTGTC TTCAGTTCGC 
CCAGCAACCA TTGACCTTCC CCCTGCAGGC CTGGTGAAAG GGGTGCACAA GGCAGCTAGA 
AGGTCTAGCT TAATGGATAC TGCAGATGGT GTTGCAGTGA ACAGTCGCGT CTCAACAAAA 
ATCCAGCAGT TGCTGAACAC GCTCAAGCGA CCAAAAAGGC CACCATTAAA GGAATTTTTT 
GTGGATGATT TTGAAGAGAT TGTAGAAGTA CCCCGACCAG ACCCAAACCA GCCCAAACCA 
GAAGGTCGCC AGGTGACCCC TGTAAAAGGA GAGCCCCTGG GTGTTGTGTG CAACTGGCCC 
CCGTCGGTAG AAGCCGCACT GCAGCGCTGG GGAACAACAC AAGCAAAATG TTCATGTCTT 
ACCGCTCTTG ATGTCACTGG CAAACCAACC TATACGTTAA CATATGGCAA GCTATGGACC 
AGGAGTGTCA GACTGGCTTA TACCCTGCTG AACAAATTGG GTACCAAGAA TGAACAAGTG 
CTGAAGCCTG GGGACAGGGT TGCCCTGGTT TACCCCAATA ATGACCCAGT GATGTTCATG 
GTTGCGTTTT ATGGCTGTCT CTTGGCGGAG GTGATCCCAG TGCCGATTGA GGACGCTGGA 
GGGCATCAGA TTGGATTCCT GCTGGGAAGC TGTGGTGTTA AGATCGCTCT TACTACTGAG 
GTTTGCTTGA AGGGGCTGCC CAAGACACAG AACGGGGAGA TTGTGCAATT CAAAGGTTGG 
CCAAAACTCA AGTGGGTTGT AACAGACTCC AGATACCTAT CAAAATCACC AAAGGACTGG 
CAGCCACACA TCACCAATGC TGGCACAGAG CCGGCATACA TTGAGTACAA AACAAGCAAG 
GAAGGCAGTG TGATGGGTGT GACCGTGTCT CGGATAGCCA TGCTGTCTCA TTGCCAGGCA 
CTCTCACAGG CCTGTAACTA CACAGAGGGT GAGACCTTAG TTAATGTCCT GGACTTCAAG 
AGGGATGCAG GCCTGTGGCA TGGTATACTA ACGAATGTAG TAAATAAAAT GCACACGATC 
AGCGTGCCGT ACGCAGTGAT GAAAGCATGT CCGTTGTCCT GGGTGCAGAG GGTACATGCC 
AGTAAAGGCA GGGTGGCTGT AGTGAAGTGC CGGGACTTGC ACTGGGCCAT GATAGCCCAC 
CGAGAACAAA AGGATATCAG TCTGGCCTCT CTCCGTATGC TCATTGTTAC AGATGGAGCT 
AATCCTTGGT CCGTTTCCTC ATGTGATGCA TTCCTGAGTT TGTTTCAGAA TCACGGCCTG 
AAGCCTGAGG TCATCTGTCC ATGTGCAACC TCTCCAGAAG CTATGACTGT CGCTATTCGA 
AGGCCTGGAG TACCCGGTGC CCCCCTGCCT GGAAGAGCCA TTTTGTCTAT GAATGGGTTA 
AGTTATGGCG TGATCAGAGT TAATACAGAG GACAAGAATT CTGCATTGAC CGTCCAGGAC 
GTTGGACATG TTATGCCAGG AAGTGTGATG TGCACTGTGA AGCCGGAAGG AGTACCCCAG 
CTCTGTAAAA CTGATGAGAT AGGAGAGATC TGTGTGAGCT GTAAAGGAGG AGGCACAATG 
TACTATGGCC TGGCAGGGCT CACCAAGAAC ACCTTTGAGG TTTTGCCAGT TAATGCTTCT 
GGGGCTTCTA TAGGAGAAAT ACCCTTCATC AGAACTGGGC TACTTGGATT TGTGGGGCCA 
GGCAGTTTAG TGTTTGTGGT GGGAAAGATG GACGGCACGG TGATGGTGAG TGGGAGGCGC 
CACAATGCTG ATGATATTGT GGCTACTGCC CTGGCGGTGG AATCCTTTAA AACCCTGTAC 
AGAGGAAGGA TTGCTGTGTT TTCAGTCACA GTATTTTATG ACGAGCGGAT TGTGGTGGTG 
GCAGAACAGC GCCCTGATGC TTCTGAGGAA GATTCCTTCC AATGGATGAG CCGTGTATTA 
CAGGCCATTG ATAGTATCCA CCAAGTGGGA GTTTACTGTC TTGCCCTGGT CCCAGCCAAT 
ACGTTGCCAA AAACTCCACT GGGAGGAATC CACATATCGC AAACTAAACA GCTTTTCTTG 
GAGGGCAGCT TACACCCCTG TAACATACTC ATGTGTCCTC ATACGTGTGT AACCAACTTG 
CCAAAACCAA GGCAGAAGCA GCCAGGAGTT GGGCCAGCAT CTGTCATGGT GGGTAATCTG 
GTCGCAGGAA AAAGAATTGC TCAGGCCTCC GGAAGAGATT TGGGACAAAT AGAGGAAAAT 
GATTTGGTGA AAAAGCACCA GTATCTGACG GAAGTCTTAC AGTGGCGAGC GCAAACCACT 
CCCGAACACG TTCTTTTTAT CCTCCTCAAT GCAAAGGGAA CGGCAGTTTG CAATGCCTCA 
TGTTTACAGC TACACAAGAG AGCAGAAAGA ATAGCAGCTG CCCTATGTGA TAAAGGTCAT 
CTCAACGCCG GCGATAACGT GGTGCTACTT TTTCCTCCCG GAATTGAGTT GATAGCTGCA 
TTCTATGGCT GCCTGTACGC TGGCTGTATT CCTGTAACTG TCCGGCCGCC TCACGCACAG 
AACCTCGCTG CCACCTTACC TACAGTACGA ATGATTGTAG ATGTTAGCAA AGCTGCCTGC 
ATTCTCACTA CACAAGCATT GCTGAGATTG CTCAAGTCCA AAGAAGCAGC AGCGGCTGTG 
GATGTGAAAA CTTGGCCAAC CATCATCGAC ACCGATGATT TGCCCAGGAA ACGGGTACCG 
CAGCTGTATA AACCACCTAC ACCTGAAATG CTGGCTTACC TGGACTTCAG TGTGAGCACG 
ACTGGCATGC TTACGGGGGT TAAGATGTCT CATGGATCAG TAAATGCACT GTGCCGGTCC 
ATAAAGCTGC AGTGTGAGCT GTACTCTACT CGCCAGATCG CCATTTGTCT TGATCCTTAT 
TGTGGGCTTG GCTTTGTACT TTGGTGCCTT TGTAGTGTTT ATTCTGGTCA TCAGTCTATA 
TTAATTCCTC CTATGGAGTT GGAATCCAAC TTATTCTTGT GGCTCTCTGC TGTGAGTCAG 
TATAAAATTA GGGACACGTT CTGTTCATAT TCTGTGATGG AGATGTGCTC AAAGGGACTG 
GGGAACCAGA TTGAGGTGCT AAAGACCAAA GGCATTAATC TGTCTTACGT GCGGACCTGT 
GTGGTGGTTG CTGAAGAGCG GCCGAGGGTG GCTTTGGCAT ATTCCTTTTC AAAACTCTTC 
AAAGACCTTG GGCTGTCCCC CCGCGCTGTG AGCACAACCT TTGGATCCAG AGTTAACACC 
GCTATATGCT TGCAGGGAAC CTCAGGGCCA GATCCTACAA CTGTGTATGT GGATCTGAAA 
TCCCTTCGGC ATGACAGGGT GCGCCTGGTG GAGAGGGGAT CTCCGCAGAG TTTGCTCCTA 
TTGGAATCTG GAAAGATTTT ACCTGGTGTC ACCGTAATTA TTGTTAATCC AGAGACCAAA 
GGACCATTGG GGGATTCCCA CCTTGGAGAG ATCTGGGTGA ACAGCGCCCA CACAGCCAGC 
GGATACTACA CAATATATGA TAGTGAGACC TTACAGGCTG ATCATTTCAA TACCAGGCTT 
AGCTTTGGAG ACACACAGAC ACTTTGGGCC AAAACGGGCT ATCTTGGCTT TGTCCGCAGG 
ACAGAACTGA CTGATGCAAA TGGAGAGCGG CACGATGCGC TGTTTGTGGT TGGAGCACTA 
GATGAAACGC TGGAGCTGAG GGGGCTTCGT TATCATCCGA TAGATATCGA GACCTCAGTG 
TCACGCATAC ACCGGAGTAT CGCTGAATGT GCTGTTTTCA CATGGACCAA CCTGCTGGTA 
GTGGTGGTGG AGCTGTGCGG CTCAGAACAG GAGGCTCTGG ACTTGGTTCC TCTGGTGACC 
AACGTGGTGT TGGAAGAACA TTACCTGATT GTGGGCGTGG TGGTGGTGGT GGATCCCGGC 
GTGATACCCA TCAATTCCCG GGGAGAGAAA CAACGGATGC ACCTGCGGGA CAGTTTCTTA 
GCGGACCAGT TGGATCCAAT CTACGTGGCG TACAACATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dip2b ( XM_018091708.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947197.1 Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X3, mRNA.

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CloneID	OXa52714
Clone ID Related Accession (Same CDS sequence)	XM_018091708.2
Accession Version	XM_018091708.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	disco-interacting protein 2 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091708.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGA GAGCCTTCGA CCTATCCGCC CTCCCGAAGG AGGTGAGGGA GCAGCTGGCA 
GAACTGGAGC TGGAGCTGTC AGAAGGTGTT GATCCAGCGC TACAGAAAGA GAATCGGAAC 
CAAACAACTG GATCACCTGC TGCTCCTGCG CAATCGTCCT CTAAGTACCA TCGAGCCAGA 
TCCGGAGGAG CTAGAGATGA GCGTTACAGA TCAGATATCC ATACAGAAGC AGTCCAAGCG 
GCTCTGGCTA AGCATAAAGA ACAGAAGATG GCATTACCAA TGCCAACCAA AAGGCGGTCA 
ACATTTGTGC AGTCTCCAGC TGACGCATGT ACCCCTCCAG ATACATCCTC CGCATCTGAG 
GACGAAGGAT CCCTAAGACG CCAGGCTGCT CTTTCGGCAG CTCTTGCTCA GAGTTTACAG 
AATTCAGAGG CCTGGATCAA CAGGTCCGTT CAGGGTTCAT CCACATCATC TTCAGCATCC 
TCCACATTAT CGCACGGAGA AGTAAAGAGT ACCAGTGGCA CCTTAGCAGA TATACTTTCT 
AATACTCACA TAGACAGTTC TCCTGCTCCA CCCGATGTTA CCAACACCAC CAATTCCACA 
ACCACTACAA GCACATCATC GTCTTCGTCA TCCTTGTCTT CAGTTCGCCC AGCAACCATT 
GACCTTCCCC CTGCAGGCCT GGTGAAAGGG GTGCACAAGG CAGCTAGAAG GTCTAGCTTA 
ATGGATACTG CAGATGGTGT TGCAGTGAAC AGTCGCGTCT CAACAAAAAT CCAGCAGTTG 
CTGAACACGC TCAAGCGACC AAAAAGGCCA CCATTAAAGG AATTTTTTGT GGATGATTTT 
GAAGAGATTG TAGAAGTACC CCGACCAGAC CCAAACCAGC CCAAACCAGA AGGTCGCCAG 
GTGACCCCTG TAAAAGGAGA GCCCCTGGGT GTTGTGTGCA ACTGGCCCCC GTCGGTAGAA 
GCCGCACTGC AGCGCTGGGG AACAACACAA GCAAAATGTT CATGTCTTAC CGCTCTTGAT 
GTCACTGGCA AACCAACCTA TACGTTAACA TATGGCAAGC TATGGACCAG GAGTGTCAGA 
CTGGCTTATA CCCTGCTGAA CAAATTGGGT ACCAAGAATG AACAAGTGCT GAAGCCTGGG 
GACAGGGTTG CCCTGGTTTA CCCCAATAAT GACCCAGTGA TGTTCATGGT TGCGTTTTAT 
GGCTGTCTCT TGGCGGAGGT GATCCCAGTG CCGATTGAGG TACCTCTTAC TAGAAAGGAC 
GCTGGAGGGC ATCAGATTGG ATTCCTGCTG GGAAGCTGTG GTGTTAAGAT CGCTCTTACT 
ACTGAGGTTT GCTTGAAGGG GCTGCCCAAG ACACAGAACG GGGAGATTGT GCAATTCAAA 
GGTTGGCCAA AACTCAAGTG GGTTGTAACA GACTCCAGAT ACCTATCAAA ATCACCAAAG 
GACTGGCAGC CACACATCAC CAATGCTGGC ACAGAGCCGG CATACATTGA GTACAAAACA 
AGCAAGGAAG GCAGTGTGAT GGGTGTGACC GTGTCTCGGA TAGCCATGCT GTCTCATTGC 
CAGGCACTCT CACAGGCCTG TAACTACACA GAGGGTGAGA CCTTAGTTAA TGTCCTGGAC 
TTCAAGAGGG ATGCAGGCCT GTGGCATGGT ATACTAACGA ATGTAGTAAA TAAAATGCAC 
ACGATCAGCG TGCCGTACGC AGTGATGAAA GCATGTCCGT TGTCCTGGGT GCAGAGGGTA 
CATGCCAGTA AAGGCAGGGT GGCTGTAGTG AAGTGCCGGG ACTTGCACTG GGCCATGATA 
GCCCACCGAG AACAAAAGGA TATCAGTCTG GCCTCTCTCC GTATGCTCAT TGTTACAGAT 
GGAGCTAATC CTTGGTCCGT TTCCTCATGT GATGCATTCC TGAGTTTGTT TCAGAATCAC 
GGCCTGAAGC CTGAGGTCAT CTGTCCATGT GCAACCTCTC CAGAAGCTAT GACTGTCGCT 
ATTCGAAGGC CTGGAGTACC CGGTGCCCCC CTGCCTGGAA GAGCCATTTT GTCTATGAAT 
GGGTTAAGTT ATGGCGTGAT CAGAGTTAAT ACAGAGGACA AGAATTCTGC ATTGACCGTC 
CAGGACGTTG GACATGTTAT GCCAGGAAGT GTGATGTGCA CTGTGAAGCC GGAAGGAGTA 
CCCCAGCTCT GTAAAACTGA TGAGATAGGA GAGATCTGTG TGAGCTGTAA AGGAGGAGGC 
ACAATGTACT ATGGCCTGGC AGGGCTCACC AAGAACACCT TTGAGGTTTT GCCAGTTAAT 
GCTTCTGGGG CTTCTATAGG AGAAATACCC TTCATCAGAA CTGGGCTACT TGGATTTGTG 
GGGCCAGGCA GTTTAGTGTT TGTGGTGGGA AAGATGGACG GCACGGTGAT GGTGAGTGGG 
AGGCGCCACA ATGCTGATGA TATTGTGGCT ACTGCCCTGG CGGTGGAATC CTTTAAAACC 
CTGTACAGAG GAAGGATTGC TGTGTTTTCA GTCACAGTAT TTTATGACGA GCGGATTGTG 
GTGGTGGCAG AACAGCGCCC TGATGCTTCT GAGGAAGATT CCTTCCAATG GATGAGCCGT 
GTATTACAGG CCATTGATAG TATCCACCAA GTGGGAGTTT ACTGTCTTGC CCTGGTCCCA 
GCCAATACGT TGCCAAAAAC TCCACTGGGA GGAATCCACA TATCGCAAAC TAAACAGCTT 
TTCTTGGAGG GCAGCTTACA CCCCTGTAAC ATACTCATGT GTCCTCATAC GTGTGTAACC 
AACTTGCCAA AACCAAGGCA GAAGCAGCCA GGAGTTGGGC CAGCATCTGT CATGGTGGGT 
AATCTGGTCG CAGGAAAAAG AATTGCTCAG GCCTCCGGAA GAGATTTGGG ACAAATAGAG 
GAAAATGATT TGGTGAAAAA GCACCAGTAT CTGACGGAAG TCTTACAGTG GCGAGCGCAA 
ACCACTCCCG AACACGTTCT TTTTATCCTC CTCAATGCAA AGGGAACGGC AGTTTGCAAT 
GCCTCATGTT TACAGCTACA CAAGAGAGCA GAAAGAATAG CAGCTGCCCT ATGTGATAAA 
GGTCATCTCA ACGCCGGCGA TAACGTGGTG CTACTTTTTC CTCCCGGAAT TGAGTTGATA 
GCTGCATTCT ATGGCTGCCT GTACGCTGGC TGTATTCCTG TAACTGTCCG GCCGCCTCAC 
GCACAGAACC TCGCTGCCAC CTTACCTACA GTACGAATGA TTGTAGATGT TAGCAAAGCT 
GCCTGCATTC TCACTACACA AGCATTGCTG AGATTGCTCA AGTCCAAAGA AGCAGCAGCG 
GCTGTGGATG TGAAAACTTG GCCAACCATC ATCGACACCG ATGATTTGCC CAGGAAACGG 
GTACCGCAGC TGTATAAACC ACCTACACCT GAAATGCTGG CTTACCTGGA CTTCAGTGTG 
AGCACGACTG GCATGCTTAC GGGGGTTAAG ATGTCTCATG GATCAGTAAA TGCACTGTGC 
CGGTCCATAA AGCTGCAGTG TGAGCTGTAC TCTACTCGCC AGATCGCCAT TTGTCTTGAT 
CCTTATTGTG GGCTTGGCTT TGTACTTTGG TGCCTTTGTA GTGTTTATTC TGGTCATCAG 
TCTATATTAA TTCCTCCTAT GGAGTTGGAA TCCAACTTAT TCTTGTGGCT CTCTGCTGTG 
AGTCAGTATA AAATTAGGGA CACGTTCTGT TCATATTCTG TGATGGAGAT GTGCTCAAAG 
GGACTGGGGA ACCAGATTGA GGTGCTAAAG ACCAAAGGCA TTAATCTGTC TTACGTGCGG 
ACCTGTGTGG TGGTTGCTGA AGAGCGGCCG AGGGTGGCTT TGGCATATTC CTTTTCAAAA 
CTCTTCAAAG ACCTTGGGCT GTCCCCCCGC GCTGTGAGCA CAACCTTTGG ATCCAGAGTT 
AACACCGCTA TATGCTTGCA GGGAACCTCA GGGCCAGATC CTACAACTGT GTATGTGGAT 
CTGAAATCCC TTCGGCATGA CAGGGTGCGC CTGGTGGAGA GGGGATCTCC GCAGAGTTTG 
CTCCTATTGG AATCTGGAAA GATTTTACCT GGTGTCACCG TAATTATTGT TAATCCAGAG 
ACCAAAGGAC CATTGGGGGA TTCCCACCTT GGAGAGATCT GGGTGAACAG CGCCCACACA 
GCCAGCGGAT ACTACACAAT ATATGATAGT GAGACCTTAC AGGCTGATCA TTTCAATACC 
AGGCTTAGCT TTGGAGACAC ACAGACACTT TGGGCCAAAA CGGGCTATCT TGGCTTTGTC 
CGCAGGACAG AACTGACTGA TGCAAATGGA GAGCGGCACG ATGCGCTGTT TGTGGTTGGA 
GCACTAGATG AAACGCTGGA GCTGAGGGGG CTTCGTTATC ATCCGATAGA TATCGAGACC 
TCAGTGTCAC GCATACACCG GAGTATCGCT GAATGTGCTG TTTTCACATG GACCAACCTG 
CTGGTAGTGG TGGTGGAGCT GTGCGGCTCA GAACAGGAGG CTCTGGACTT GGTTCCTCTG 
GTGACCAACG TGGTGTTGGA AGAACATTAC CTGATTGTGG GCGTGGTGGT GGTGGTGGAT 
CCCGGCGTGA TACCCATCAA TTCCCGGGGA GAGAAACAAC GGATGCACCT GCGGGACAGT 
TTCTTAGCGG ACCAGTTGGA TCCAATCTAC GTGGCGTACA ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947197.1
CDS	188..4855
Translation	MAERAFDLSALPKEVREQLAELELELSEGVDPALQKENRNQTTGSPAAPAQSSSKYHRARSGGARDERYRSDIHTEAVQAALAKHKEQKMALPMPTKRRSTFVQSPADACTPPDTSSASEDEGSLRRQAALSAALAQSLQNSEAWINRSVQGSSTSSSASSTLSHGEVKSTSGTLADILSNTHIDSSPAPPDVTNTTNSTTTTSTSSSSSSLSSVRPATIDLPPAGLVKGVHKAARRSSLMDTADGVAVNSRVSTKIQQLLNTLKRPKRPPLKEFFVDDFEEIVEVPRPDPNQPKPEGRQVTPVKGEPLGVVCNWPPSVEAALQRWGTTQAKCSCLTALDVTGKPTYTLTYGKLWTRSVRLAYTLLNKLGTKNEQVLKPGDRVALVYPNNDPVMFMVAFYGCLLAEVIPVPIEVPLTRKDAGGHQIGFLLGSCGVKIALTTEVCLKGLPKTQNGEIVQFKGWPKLKWVVTDSRYLSKSPKDWQPHITNAGTEPAYIEYKTSKEGSVMGVTVSRIAMLSHCQALSQACNYTEGETLVNVLDFKRDAGLWHGILTNVVNKMHTISVPYAVMKACPLSWVQRVHASKGRVAVVKCRDLHWAMIAHREQKDISLASLRMLIVTDGANPWSVSSCDAFLSLFQNHGLKPEVICPCATSPEAMTVAIRRPGVPGAPLPGRAILSMNGLSYGVIRVNTEDKNSALTVQDVGHVMPGSVMCTVKPEGVPQLCKTDEIGEICVSCKGGGTMYYGLAGLTKNTFEVLPVNASGASIGEIPFIRTGLLGFVGPGSLVFVVGKMDGTVMVSGRRHNADDIVATALAVESFKTLYRGRIAVFSVTVFYDERIVVVAEQRPDASEEDSFQWMSRVLQAIDSIHQVGVYCLALVPANTLPKTPLGGIHISQTKQLFLEGSLHPCNILMCPHTCVTNLPKPRQKQPGVGPASVMVGNLVAGKRIAQASGRDLGQIEENDLVKKHQYLTEVLQWRAQTTPEHVLFILLNAKGTAVCNASCLQLHKRAERIAAALCDKGHLNAGDNVVLLFPPGIELIAAFYGCLYAGCIPVTVRPPHAQNLAATLPTVRMIVDVSKAACILTTQALLRLLKSKEAAAAVDVKTWPTIIDTDDLPRKRVPQLYKPPTPEMLAYLDFSVSTTGMLTGVKMSHGSVNALCRSIKLQCELYSTRQIAICLDPYCGLGFVLWCLCSVYSGHQSILIPPMELESNLFLWLSAVSQYKIRDTFCSYSVMEMCSKGLGNQIEVLKTKGINLSYVRTCVVVAEERPRVALAYSFSKLFKDLGLSPRAVSTTFGSRVNTAICLQGTSGPDPTTVYVDLKSLRHDRVRLVERGSPQSLLLLESGKILPGVTVIIVNPETKGPLGDSHLGEIWVNSAHTASGYYTIYDSETLQADHFNTRLSFGDTQTLWAKTGYLGFVRRTELTDANGERHDALFVVGALDETLELRGLRYHPIDIETSVSRIHRSIAECAVFTWTNLLVVVVELCGSEQEALDLVPLVTNVVLEEHYLIVGVVVVVDPGVIPINSRGEKQRMHLRDSFLADQLDPIYVAYNM

Target ORF information:

RefSeq Version	XM_018091708.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091708.2

ORF Insert Sequence:

ATGGCGGAGA GAGCCTTCGA CCTATCCGCC CTCCCGAAGG AGGTGAGGGA GCAGCTGGCA 
GAACTGGAGC TGGAGCTGTC AGAAGGTGTT GATCCAGCGC TACAGAAAGA GAATCGGAAC 
CAAACAACTG GATCACCTGC TGCTCCTGCG CAATCGTCCT CTAAGTACCA TCGAGCCAGA 
TCCGGAGGAG CTAGAGATGA GCGTTACAGA TCAGATATCC ATACAGAAGC AGTCCAAGCG 
GCTCTGGCTA AGCATAAAGA ACAGAAGATG GCATTACCAA TGCCAACCAA AAGGCGGTCA 
ACATTTGTGC AGTCTCCAGC TGACGCATGT ACCCCTCCAG ATACATCCTC CGCATCTGAG 
GACGAAGGAT CCCTAAGACG CCAGGCTGCT CTTTCGGCAG CTCTTGCTCA GAGTTTACAG 
AATTCAGAGG CCTGGATCAA CAGGTCCGTT CAGGGTTCAT CCACATCATC TTCAGCATCC 
TCCACATTAT CGCACGGAGA AGTAAAGAGT ACCAGTGGCA CCTTAGCAGA TATACTTTCT 
AATACTCACA TAGACAGTTC TCCTGCTCCA CCCGATGTTA CCAACACCAC CAATTCCACA 
ACCACTACAA GCACATCATC GTCTTCGTCA TCCTTGTCTT CAGTTCGCCC AGCAACCATT 
GACCTTCCCC CTGCAGGCCT GGTGAAAGGG GTGCACAAGG CAGCTAGAAG GTCTAGCTTA 
ATGGATACTG CAGATGGTGT TGCAGTGAAC AGTCGCGTCT CAACAAAAAT CCAGCAGTTG 
CTGAACACGC TCAAGCGACC AAAAAGGCCA CCATTAAAGG AATTTTTTGT GGATGATTTT 
GAAGAGATTG TAGAAGTACC CCGACCAGAC CCAAACCAGC CCAAACCAGA AGGTCGCCAG 
GTGACCCCTG TAAAAGGAGA GCCCCTGGGT GTTGTGTGCA ACTGGCCCCC GTCGGTAGAA 
GCCGCACTGC AGCGCTGGGG AACAACACAA GCAAAATGTT CATGTCTTAC CGCTCTTGAT 
GTCACTGGCA AACCAACCTA TACGTTAACA TATGGCAAGC TATGGACCAG GAGTGTCAGA 
CTGGCTTATA CCCTGCTGAA CAAATTGGGT ACCAAGAATG AACAAGTGCT GAAGCCTGGG 
GACAGGGTTG CCCTGGTTTA CCCCAATAAT GACCCAGTGA TGTTCATGGT TGCGTTTTAT 
GGCTGTCTCT TGGCGGAGGT GATCCCAGTG CCGATTGAGG TACCTCTTAC TAGAAAGGAC 
GCTGGAGGGC ATCAGATTGG ATTCCTGCTG GGAAGCTGTG GTGTTAAGAT CGCTCTTACT 
ACTGAGGTTT GCTTGAAGGG GCTGCCCAAG ACACAGAACG GGGAGATTGT GCAATTCAAA 
GGTTGGCCAA AACTCAAGTG GGTTGTAACA GACTCCAGAT ACCTATCAAA ATCACCAAAG 
GACTGGCAGC CACACATCAC CAATGCTGGC ACAGAGCCGG CATACATTGA GTACAAAACA 
AGCAAGGAAG GCAGTGTGAT GGGTGTGACC GTGTCTCGGA TAGCCATGCT GTCTCATTGC 
CAGGCACTCT CACAGGCCTG TAACTACACA GAGGGTGAGA CCTTAGTTAA TGTCCTGGAC 
TTCAAGAGGG ATGCAGGCCT GTGGCATGGT ATACTAACGA ATGTAGTAAA TAAAATGCAC 
ACGATCAGCG TGCCGTACGC AGTGATGAAA GCATGTCCGT TGTCCTGGGT GCAGAGGGTA 
CATGCCAGTA AAGGCAGGGT GGCTGTAGTG AAGTGCCGGG ACTTGCACTG GGCCATGATA 
GCCCACCGAG AACAAAAGGA TATCAGTCTG GCCTCTCTCC GTATGCTCAT TGTTACAGAT 
GGAGCTAATC CTTGGTCCGT TTCCTCATGT GATGCATTCC TGAGTTTGTT TCAGAATCAC 
GGCCTGAAGC CTGAGGTCAT CTGTCCATGT GCAACCTCTC CAGAAGCTAT GACTGTCGCT 
ATTCGAAGGC CTGGAGTACC CGGTGCCCCC CTGCCTGGAA GAGCCATTTT GTCTATGAAT 
GGGTTAAGTT ATGGCGTGAT CAGAGTTAAT ACAGAGGACA AGAATTCTGC ATTGACCGTC 
CAGGACGTTG GACATGTTAT GCCAGGAAGT GTGATGTGCA CTGTGAAGCC GGAAGGAGTA 
CCCCAGCTCT GTAAAACTGA TGAGATAGGA GAGATCTGTG TGAGCTGTAA AGGAGGAGGC 
ACAATGTACT ATGGCCTGGC AGGGCTCACC AAGAACACCT TTGAGGTTTT GCCAGTTAAT 
GCTTCTGGGG CTTCTATAGG AGAAATACCC TTCATCAGAA CTGGGCTACT TGGATTTGTG 
GGGCCAGGCA GTTTAGTGTT TGTGGTGGGA AAGATGGACG GCACGGTGAT GGTGAGTGGG 
AGGCGCCACA ATGCTGATGA TATTGTGGCT ACTGCCCTGG CGGTGGAATC CTTTAAAACC 
CTGTACAGAG GAAGGATTGC TGTGTTTTCA GTCACAGTAT TTTATGACGA GCGGATTGTG 
GTGGTGGCAG AACAGCGCCC TGATGCTTCT GAGGAAGATT CCTTCCAATG GATGAGCCGT 
GTATTACAGG CCATTGATAG TATCCACCAA GTGGGAGTTT ACTGTCTTGC CCTGGTCCCA 
GCCAATACGT TGCCAAAAAC TCCACTGGGA GGAATCCACA TATCGCAAAC TAAACAGCTT 
TTCTTGGAGG GCAGCTTACA CCCCTGTAAC ATACTCATGT GTCCTCATAC GTGTGTAACC 
AACTTGCCAA AACCAAGGCA GAAGCAGCCA GGAGTTGGGC CAGCATCTGT CATGGTGGGT 
AATCTGGTCG CAGGAAAAAG AATTGCTCAG GCCTCCGGAA GAGATTTGGG ACAAATAGAG 
GAAAATGATT TGGTGAAAAA GCACCAGTAT CTGACGGAAG TCTTACAGTG GCGAGCGCAA 
ACCACTCCCG AACACGTTCT TTTTATCCTC CTCAATGCAA AGGGAACGGC AGTTTGCAAT 
GCCTCATGTT TACAGCTACA CAAGAGAGCA GAAAGAATAG CAGCTGCCCT ATGTGATAAA 
GGTCATCTCA ACGCCGGCGA TAACGTGGTG CTACTTTTTC CTCCCGGAAT TGAGTTGATA 
GCTGCATTCT ATGGCTGCCT GTACGCTGGC TGTATTCCTG TAACTGTCCG GCCGCCTCAC 
GCACAGAACC TCGCTGCCAC CTTACCTACA GTACGAATGA TTGTAGATGT TAGCAAAGCT 
GCCTGCATTC TCACTACACA AGCATTGCTG AGATTGCTCA AGTCCAAAGA AGCAGCAGCG 
GCTGTGGATG TGAAAACTTG GCCAACCATC ATCGACACCG ATGATTTGCC CAGGAAACGG 
GTACCGCAGC TGTATAAACC ACCTACACCT GAAATGCTGG CTTACCTGGA CTTCAGTGTG 
AGCACGACTG GCATGCTTAC GGGGGTTAAG ATGTCTCATG GATCAGTAAA TGCACTGTGC 
CGGTCCATAA AGCTGCAGTG TGAGCTGTAC TCTACTCGCC AGATCGCCAT TTGTCTTGAT 
CCTTATTGTG GGCTTGGCTT TGTACTTTGG TGCCTTTGTA GTGTTTATTC TGGTCATCAG 
TCTATATTAA TTCCTCCTAT GGAGTTGGAA TCCAACTTAT TCTTGTGGCT CTCTGCTGTG 
AGTCAGTATA AAATTAGGGA CACGTTCTGT TCATATTCTG TGATGGAGAT GTGCTCAAAG 
GGACTGGGGA ACCAGATTGA GGTGCTAAAG ACCAAAGGCA TTAATCTGTC TTACGTGCGG 
ACCTGTGTGG TGGTTGCTGA AGAGCGGCCG AGGGTGGCTT TGGCATATTC CTTTTCAAAA 
CTCTTCAAAG ACCTTGGGCT GTCCCCCCGC GCTGTGAGCA CAACCTTTGG ATCCAGAGTT 
AACACCGCTA TATGCTTGCA GGGAACCTCA GGGCCAGATC CTACAACTGT GTATGTGGAT 
CTGAAATCCC TTCGGCATGA CAGGGTGCGC CTGGTGGAGA GGGGATCTCC GCAGAGTTTG 
CTCCTATTGG AATCTGGAAA GATTTTACCT GGTGTCACCG TAATTATTGT TAATCCAGAG 
ACCAAAGGAC CATTGGGGGA TTCCCACCTT GGAGAGATCT GGGTGAACAG CGCCCACACA 
GCCAGCGGAT ACTACACAAT ATATGATAGT GAGACCTTAC AGGCTGATCA TTTCAATACC 
AGGCTTAGCT TTGGAGACAC ACAGACACTT TGGGCCAAAA CGGGCTATCT TGGCTTTGTC 
CGCAGGACAG AACTGACTGA TGCAAATGGA GAGCGGCACG ATGCGCTGTT TGTGGTTGGA 
GCACTAGATG AAACGCTGGA GCTGAGGGGG CTTCGTTATC ATCCGATAGA TATCGAGACC 
TCAGTGTCAC GCATACACCG GAGTATCGCT GAATGTGCTG TTTTCACATG GACCAACCTG 
CTGGTAGTGG TGGTGGAGCT GTGCGGCTCA GAACAGGAGG CTCTGGACTT GGTTCCTCTG 
GTGACCAACG TGGTGTTGGA AGAACATTAC CTGATTGTGG GCGTGGTGGT GGTGGTGGAT 
CCCGGCGTGA TACCCATCAA TTCCCGGGGA GAGAAACAAC GGATGCACCT GCGGGACAGT 
TTCTTAGCGG ACCAGTTGGA TCCAATCTAC GTGGCGTACA ACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dip2b ( XM_002933708.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002933754.2 Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X1, mRNA.

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CloneID	OXa06062
Clone ID Related Accession (Same CDS sequence)	XM_002933708.4
Accession Version	XM_002933708.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	disco-interacting protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002933708.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGA GAGCCTTCGA CCTATCCGCC CTCCCGAAGG AGGTGAGGGA GCAGCTGGCA 
GAACTGGAGC TGGAGCTGTC AGAAGGTGAT ATCACCCAGA AGGGTTATGA GAAAAAAAGA 
GCAAAACTAT TGGGTCCGTA CATCCCGCAA ACACAAGGTG TTGATCCAGC GCTACAGAAA 
GAGAATCGGA ACCAAACAAC TGGATCACCT GCTGCTCCTG CGCAATCGTC CTCTAAGTAC 
CATCGAGCCA GATCCGGAGG AGCTAGAGAT GAGCGTTACA GATCAGATAT CCATACAGAA 
GCAGTCCAAG CGGCTCTGGC TAAGCATAAA GAACAGAAGA TGGCATTACC AATGCCAACC 
AAAAGGCGGT CAACATTTGT GCAGTCTCCA GCTGACGCAT GTACCCCTCC AGATACATCC 
TCCGCATCTG AGGACGAAGG ATCCCTAAGA CGCCAGGCTG CTCTTTCGGC AGCTCTTGCT 
CAGAGTTTAC AGAATTCAGA GGCCTGGATC AACAGGTCCG TTCAGGGTTC ATCCACATCA 
TCTTCAGCAT CCTCCACATT ATCGCACGGA GAAGTAAAGA GTACCAGTGG CACCTTAGCA 
GATATACTTT CTAATACTCA CATAGACAGT TCTCCTGCTC CACCCGATGT TACCAACACC 
ACCAATTCCA CAACCACTAC AAGCACATCA TCGTCTTCGT CATCCTTGTC TTCAGTTCGC 
CCAGCAACCA TTGACCTTCC CCCTGCAGGC CTGGTGAAAG GGGTGCACAA GGCAGCTAGA 
AGGTCTAGCT TAATGGATAC TGCAGATGGT GTTGCAGTGA ACAGTCGCGT CTCAACAAAA 
ATCCAGCAGT TGCTGAACAC GCTCAAGCGA CCAAAAAGGC CACCATTAAA GGAATTTTTT 
GTGGATGATT TTGAAGAGAT TGTAGAAGTA CCCCGACCAG ACCCAAACCA GCCCAAACCA 
GAAGGTCGCC AGGTGACCCC TGTAAAAGGA GAGCCCCTGG GTGTTGTGTG CAACTGGCCC 
CCGTCGGTAG AAGCCGCACT GCAGCGCTGG GGAACAACAC AAGCAAAATG TTCATGTCTT 
ACCGCTCTTG ATGTCACTGG CAAACCAACC TATACGTTAA CATATGGCAA GCTATGGACC 
AGGAGTGTCA GACTGGCTTA TACCCTGCTG AACAAATTGG GTACCAAGAA TGAACAAGTG 
CTGAAGCCTG GGGACAGGGT TGCCCTGGTT TACCCCAATA ATGACCCAGT GATGTTCATG 
GTTGCGTTTT ATGGCTGTCT CTTGGCGGAG GTGATCCCAG TGCCGATTGA GGTACCTCTT 
ACTAGAAAGG ACGCTGGAGG GCATCAGATT GGATTCCTGC TGGGAAGCTG TGGTGTTAAG 
ATCGCTCTTA CTACTGAGGT TTGCTTGAAG GGGCTGCCCA AGACACAGAA CGGGGAGATT 
GTGCAATTCA AAGGTTGGCC AAAACTCAAG TGGGTTGTAA CAGACTCCAG ATACCTATCA 
AAATCACCAA AGGACTGGCA GCCACACATC ACCAATGCTG GCACAGAGCC GGCATACATT 
GAGTACAAAA CAAGCAAGGA AGGCAGTGTG ATGGGTGTGA CCGTGTCTCG GATAGCCATG 
CTGTCTCATT GCCAGGCACT CTCACAGGCC TGTAACTACA CAGAGGGTGA GACCTTAGTT 
AATGTCCTGG ACTTCAAGAG GGATGCAGGC CTGTGGCATG GTATACTAAC GAATGTAGTA 
AATAAAATGC ACACGATCAG CGTGCCGTAC GCAGTGATGA AAGCATGTCC GTTGTCCTGG 
GTGCAGAGGG TACATGCCAG TAAAGGCAGG GTGGCTGTAG TGAAGTGCCG GGACTTGCAC 
TGGGCCATGA TAGCCCACCG AGAACAAAAG GATATCAGTC TGGCCTCTCT CCGTATGCTC 
ATTGTTACAG ATGGAGCTAA TCCTTGGTCC GTTTCCTCAT GTGATGCATT CCTGAGTTTG 
TTTCAGAATC ACGGCCTGAA GCCTGAGGTC ATCTGTCCAT GTGCAACCTC TCCAGAAGCT 
ATGACTGTCG CTATTCGAAG GCCTGGAGTA CCCGGTGCCC CCCTGCCTGG AAGAGCCATT 
TTGTCTATGA ATGGGTTAAG TTATGGCGTG ATCAGAGTTA ATACAGAGGA CAAGAATTCT 
GCATTGACCG TCCAGGACGT TGGACATGTT ATGCCAGGAA GTGTGATGTG CACTGTGAAG 
CCGGAAGGAG TACCCCAGCT CTGTAAAACT GATGAGATAG GAGAGATCTG TGTGAGCTGT 
AAAGGAGGAG GCACAATGTA CTATGGCCTG GCAGGGCTCA CCAAGAACAC CTTTGAGGTT 
TTGCCAGTTA ATGCTTCTGG GGCTTCTATA GGAGAAATAC CCTTCATCAG AACTGGGCTA 
CTTGGATTTG TGGGGCCAGG CAGTTTAGTG TTTGTGGTGG GAAAGATGGA CGGCACGGTG 
ATGGTGAGTG GGAGGCGCCA CAATGCTGAT GATATTGTGG CTACTGCCCT GGCGGTGGAA 
TCCTTTAAAA CCCTGTACAG AGGAAGGATT GCTGTGTTTT CAGTCACAGT ATTTTATGAC 
GAGCGGATTG TGGTGGTGGC AGAACAGCGC CCTGATGCTT CTGAGGAAGA TTCCTTCCAA 
TGGATGAGCC GTGTATTACA GGCCATTGAT AGTATCCACC AAGTGGGAGT TTACTGTCTT 
GCCCTGGTTC CAGCCAATAC GTTGCCAAAA ACTCCACTGG GAGGAATCCA CATATCGCAA 
ACTAAACAGC TTTTCTTGGA GGGCAGCTTA CACCCCTGTA ACATACTCAT GTGTCCTCAT 
ACGTGTGTAA CCAACTTGCC AAAACCAAGG CAGAAGCAGC CAGGAGTTGG GCCAGCATCT 
GTCATGGTGG GTAATCTGGT CGCAGGAAAA AGAATTGCTC AGGCCTCCGG AAGAGATTTG 
GGACAAATAG AGGAAAATGA TTTGGTGAAA AAGCACCAGT ATCTGACGGA AGTCTTACAG 
TGGCGAGCGC AAACCACTCC CGAACACGTT CTTTTTATCC TCCTCAATGC AAAGGGAACG 
GCAGTTTGCA ATGCCTCATG TTTACAGCTA CACAAGCGAG CAGAAAGAAT AGCAGCTGCC 
CTATGTGATA AAGGTCATCT CAACGCCGGC GATAACGTGG TGCTACTTTT TCCTCCCGGA 
ATTGAGTTGA TAGCTGCATT CTATGGCTGC CTGTACGCTG GCTGTATTCC TGTAACTGTC 
CGGCCGCCTC ACGCACAGAA CCTCGCTGCC ACCTTACCTA CAGTACGAAT GATTGTAGAT 
GTTAGCAAAG CTGCCTGCAT TCTCACTACA CAAGCATTGC TGAGATTGCT CAAGTCCAAA 
GAAGCAGCAG CGGCTGTGGA TGTGAAAACT TGGCCAACCA TCATCGACAC CGATGATTTG 
CCCAGGAAAC GGGTACCGCA GCTGTATAAA CCACCTACAC CTGAAATGCT GGCTTACCTG 
GACTTCAGTG TGAGCACGAC TGGCATGCTT ACGGGGGTTA AGATGTCTCA TGGATCAGTA 
AATGCACTGT GCCGGTCCAT AAAGCTGCAG TGTGAGCTGT ACTCTACTCG TCAGATCGCC 
ATTTGTCTTG ATCCTTATTG TGGGCTTGGC TTTGTACTTT GGTGCCTTTG TAGTGTTTAT 
TCTGGTCATC AGTCTATATT AATTCCTCCT ATGGAGTTGG AATCCAACTT ATTCTTGTGG 
CTCTCTGCTG TGAGTCAGTA TAAAATTAGG GACACGTTCT GTTCATATTC TGTGATGGAG 
ATGTGCTCAA AGGGACTGGG GAACCAGATT GAGGTGCTAA AGACCAAAGG CATTAATCTG 
TCTTACGTGC GGACCTGTGT GGTGGTTGCT GAAGAGCGGC CGAGGGTGGC TTTGGCATAT 
TCCTTTTCAA AACTCTTCAA AGACCTTGGG CTGTCCCCCC GCGCTGTGAG CACAACCTTT 
GGATCCAGAG TTAACACCGC TATATGCTTG CAGGGAACCT CAGGGCCAGA TCCAACAACT 
GTGTATGTGG ATCTGAAATC CCTTCGGCAT GACAGGGTGC GCCTGGTGGA GAGGGGATCT 
CCGCAGAGTT TGCTCCTATT GGAATCTGGA AAGATTTTAC CTGGTGTCAC CGTAATTATT 
GTTAATCCAG AGACCAAAGG ACCATTGGGG GATTCCCACC TTGGAGAGAT CTGGGTGAAC 
AGCGCCCACA CAGCCAGCGG ATACTACACA ATATATGATA GTGAGACCTT ACAGGCTGAT 
CATTTCAATA CTAGGCTTAG CTTTGGAGAC ACACAGACAC TTTGGGCCAA AACGGGCTAT 
CTTGGCTTTG TCCGCAGGAC AGAACTGACT GATGCAAATG GAGAGCGGCA CGATGCGCTG 
TTTGTGGTTG GAGCACTAGA TGAAACGCTG GAGCTGAGGG GGCTTCGTTA TCATCCGATA 
GATATCGAGA CCTCAGTGTC ACGCATACAC CGGAGTATCG CTGAATGTGC TGTTTTCACA 
TGGACCAACC TGCTGGTAGT GGTGGTGGAG CTGTGCGGCT CAGAACAGGA GGCTCTGGAC 
TTGGTTCCTC TGGTGACCAA CGTGGTGTTG GAAGAACATT ACCTGATTGT GGGCGTGGTG 
GTGGTGGTGG ATCCCGGCGT GATACCCATC AATTCCCGGG GAGAGAAACA ACGGATGCAC 
CTGCGGGACA GTTTCTTAGC GGACCAGTTG GATCCAATCT ACGTGGCGTA CAACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002933754.2
CDS	203..4942
Translation	MAERAFDLSALPKEVREQLAELELELSEGDITQKGYEKKRAKLLGPYIPQTQGVDPALQKENRNQTTGSPAAPAQSSSKYHRARSGGARDERYRSDIHTEAVQAALAKHKEQKMALPMPTKRRSTFVQSPADACTPPDTSSASEDEGSLRRQAALSAALAQSLQNSEAWINRSVQGSSTSSSASSTLSHGEVKSTSGTLADILSNTHIDSSPAPPDVTNTTNSTTTTSTSSSSSSLSSVRPATIDLPPAGLVKGVHKAARRSSLMDTADGVAVNSRVSTKIQQLLNTLKRPKRPPLKEFFVDDFEEIVEVPRPDPNQPKPEGRQVTPVKGEPLGVVCNWPPSVEAALQRWGTTQAKCSCLTALDVTGKPTYTLTYGKLWTRSVRLAYTLLNKLGTKNEQVLKPGDRVALVYPNNDPVMFMVAFYGCLLAEVIPVPIEVPLTRKDAGGHQIGFLLGSCGVKIALTTEVCLKGLPKTQNGEIVQFKGWPKLKWVVTDSRYLSKSPKDWQPHITNAGTEPAYIEYKTSKEGSVMGVTVSRIAMLSHCQALSQACNYTEGETLVNVLDFKRDAGLWHGILTNVVNKMHTISVPYAVMKACPLSWVQRVHASKGRVAVVKCRDLHWAMIAHREQKDISLASLRMLIVTDGANPWSVSSCDAFLSLFQNHGLKPEVICPCATSPEAMTVAIRRPGVPGAPLPGRAILSMNGLSYGVIRVNTEDKNSALTVQDVGHVMPGSVMCTVKPEGVPQLCKTDEIGEICVSCKGGGTMYYGLAGLTKNTFEVLPVNASGASIGEIPFIRTGLLGFVGPGSLVFVVGKMDGTVMVSGRRHNADDIVATALAVESFKTLYRGRIAVFSVTVFYDERIVVVAEQRPDASEEDSFQWMSRVLQAIDSIHQVGVYCLALVPANTLPKTPLGGIHISQTKQLFLEGSLHPCNILMCPHTCVTNLPKPRQKQPGVGPASVMVGNLVAGKRIAQASGRDLGQIEENDLVKKHQYLTEVLQWRAQTTPEHVLFILLNAKGTAVCNASCLQLHKRAERIAAALCDKGHLNAGDNVVLLFPPGIELIAAFYGCLYAGCIPVTVRPPHAQNLAATLPTVRMIVDVSKAACILTTQALLRLLKSKEAAAAVDVKTWPTIIDTDDLPRKRVPQLYKPPTPEMLAYLDFSVSTTGMLTGVKMSHGSVNALCRSIKLQCELYSTRQIAICLDPYCGLGFVLWCLCSVYSGHQSILIPPMELESNLFLWLSAVSQYKIRDTFCSYSVMEMCSKGLGNQIEVLKTKGINLSYVRTCVVVAEERPRVALAYSFSKLFKDLGLSPRAVSTTFGSRVNTAICLQGTSGPDPTTVYVDLKSLRHDRVRLVERGSPQSLLLLESGKILPGVTVIIVNPETKGPLGDSHLGEIWVNSAHTASGYYTIYDSETLQADHFNTRLSFGDTQTLWAKTGYLGFVRRTELTDANGERHDALFVVGALDETLELRGLRYHPIDIETSVSRIHRSIAECAVFTWTNLLVVVVELCGSEQEALDLVPLVTNVVLEEHYLIVGVVVVVDPGVIPINSRGEKQRMHLRDSFLADQLDPIYVAYNM

Target ORF information:

RefSeq Version	XM_002933708.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002933708.4

ORF Insert Sequence:

ATGGCGGAGA GAGCCTTCGA CCTATCCGCC CTCCCGAAGG AGGTGAGGGA GCAGCTGGCA 
GAACTGGAGC TGGAGCTGTC AGAAGGTGAT ATCACCCAGA AGGGTTATGA GAAAAAAAGA 
GCAAAACTAT TGGGTCCGTA CATCCCGCAA ACACAAGGTG TTGATCCAGC GCTACAGAAA 
GAGAATCGGA ACCAAACAAC TGGATCACCT GCTGCTCCTG CGCAATCGTC CTCTAAGTAC 
CATCGAGCCA GATCCGGAGG AGCTAGAGAT GAGCGTTACA GATCAGATAT CCATACAGAA 
GCAGTCCAAG CGGCTCTGGC TAAGCATAAA GAACAGAAGA TGGCATTACC AATGCCAACC 
AAAAGGCGGT CAACATTTGT GCAGTCTCCA GCTGACGCAT GTACCCCTCC AGATACATCC 
TCCGCATCTG AGGACGAAGG ATCCCTAAGA CGCCAGGCTG CTCTTTCGGC AGCTCTTGCT 
CAGAGTTTAC AGAATTCAGA GGCCTGGATC AACAGGTCCG TTCAGGGTTC ATCCACATCA 
TCTTCAGCAT CCTCCACATT ATCGCACGGA GAAGTAAAGA GTACCAGTGG CACCTTAGCA 
GATATACTTT CTAATACTCA CATAGACAGT TCTCCTGCTC CACCCGATGT TACCAACACC 
ACCAATTCCA CAACCACTAC AAGCACATCA TCGTCTTCGT CATCCTTGTC TTCAGTTCGC 
CCAGCAACCA TTGACCTTCC CCCTGCAGGC CTGGTGAAAG GGGTGCACAA GGCAGCTAGA 
AGGTCTAGCT TAATGGATAC TGCAGATGGT GTTGCAGTGA ACAGTCGCGT CTCAACAAAA 
ATCCAGCAGT TGCTGAACAC GCTCAAGCGA CCAAAAAGGC CACCATTAAA GGAATTTTTT 
GTGGATGATT TTGAAGAGAT TGTAGAAGTA CCCCGACCAG ACCCAAACCA GCCCAAACCA 
GAAGGTCGCC AGGTGACCCC TGTAAAAGGA GAGCCCCTGG GTGTTGTGTG CAACTGGCCC 
CCGTCGGTAG AAGCCGCACT GCAGCGCTGG GGAACAACAC AAGCAAAATG TTCATGTCTT 
ACCGCTCTTG ATGTCACTGG CAAACCAACC TATACGTTAA CATATGGCAA GCTATGGACC 
AGGAGTGTCA GACTGGCTTA TACCCTGCTG AACAAATTGG GTACCAAGAA TGAACAAGTG 
CTGAAGCCTG GGGACAGGGT TGCCCTGGTT TACCCCAATA ATGACCCAGT GATGTTCATG 
GTTGCGTTTT ATGGCTGTCT CTTGGCGGAG GTGATCCCAG TGCCGATTGA GGTACCTCTT 
ACTAGAAAGG ACGCTGGAGG GCATCAGATT GGATTCCTGC TGGGAAGCTG TGGTGTTAAG 
ATCGCTCTTA CTACTGAGGT TTGCTTGAAG GGGCTGCCCA AGACACAGAA CGGGGAGATT 
GTGCAATTCA AAGGTTGGCC AAAACTCAAG TGGGTTGTAA CAGACTCCAG ATACCTATCA 
AAATCACCAA AGGACTGGCA GCCACACATC ACCAATGCTG GCACAGAGCC GGCATACATT 
GAGTACAAAA CAAGCAAGGA AGGCAGTGTG ATGGGTGTGA CCGTGTCTCG GATAGCCATG 
CTGTCTCATT GCCAGGCACT CTCACAGGCC TGTAACTACA CAGAGGGTGA GACCTTAGTT 
AATGTCCTGG ACTTCAAGAG GGATGCAGGC CTGTGGCATG GTATACTAAC GAATGTAGTA 
AATAAAATGC ACACGATCAG CGTGCCGTAC GCAGTGATGA AAGCATGTCC GTTGTCCTGG 
GTGCAGAGGG TACATGCCAG TAAAGGCAGG GTGGCTGTAG TGAAGTGCCG GGACTTGCAC 
TGGGCCATGA TAGCCCACCG AGAACAAAAG GATATCAGTC TGGCCTCTCT CCGTATGCTC 
ATTGTTACAG ATGGAGCTAA TCCTTGGTCC GTTTCCTCAT GTGATGCATT CCTGAGTTTG 
TTTCAGAATC ACGGCCTGAA GCCTGAGGTC ATCTGTCCAT GTGCAACCTC TCCAGAAGCT 
ATGACTGTCG CTATTCGAAG GCCTGGAGTA CCCGGTGCCC CCCTGCCTGG AAGAGCCATT 
TTGTCTATGA ATGGGTTAAG TTATGGCGTG ATCAGAGTTA ATACAGAGGA CAAGAATTCT 
GCATTGACCG TCCAGGACGT TGGACATGTT ATGCCAGGAA GTGTGATGTG CACTGTGAAG 
CCGGAAGGAG TACCCCAGCT CTGTAAAACT GATGAGATAG GAGAGATCTG TGTGAGCTGT 
AAAGGAGGAG GCACAATGTA CTATGGCCTG GCAGGGCTCA CCAAGAACAC CTTTGAGGTT 
TTGCCAGTTA ATGCTTCTGG GGCTTCTATA GGAGAAATAC CCTTCATCAG AACTGGGCTA 
CTTGGATTTG TGGGGCCAGG CAGTTTAGTG TTTGTGGTGG GAAAGATGGA CGGCACGGTG 
ATGGTGAGTG GGAGGCGCCA CAATGCTGAT GATATTGTGG CTACTGCCCT GGCGGTGGAA 
TCCTTTAAAA CCCTGTACAG AGGAAGGATT GCTGTGTTTT CAGTCACAGT ATTTTATGAC 
GAGCGGATTG TGGTGGTGGC AGAACAGCGC CCTGATGCTT CTGAGGAAGA TTCCTTCCAA 
TGGATGAGCC GTGTATTACA GGCCATTGAT AGTATCCACC AAGTGGGAGT TTACTGTCTT 
GCCCTGGTTC CAGCCAATAC GTTGCCAAAA ACTCCACTGG GAGGAATCCA CATATCGCAA 
ACTAAACAGC TTTTCTTGGA GGGCAGCTTA CACCCCTGTA ACATACTCAT GTGTCCTCAT 
ACGTGTGTAA CCAACTTGCC AAAACCAAGG CAGAAGCAGC CAGGAGTTGG GCCAGCATCT 
GTCATGGTGG GTAATCTGGT CGCAGGAAAA AGAATTGCTC AGGCCTCCGG AAGAGATTTG 
GGACAAATAG AGGAAAATGA TTTGGTGAAA AAGCACCAGT ATCTGACGGA AGTCTTACAG 
TGGCGAGCGC AAACCACTCC CGAACACGTT CTTTTTATCC TCCTCAATGC AAAGGGAACG 
GCAGTTTGCA ATGCCTCATG TTTACAGCTA CACAAGCGAG CAGAAAGAAT AGCAGCTGCC 
CTATGTGATA AAGGTCATCT CAACGCCGGC GATAACGTGG TGCTACTTTT TCCTCCCGGA 
ATTGAGTTGA TAGCTGCATT CTATGGCTGC CTGTACGCTG GCTGTATTCC TGTAACTGTC 
CGGCCGCCTC ACGCACAGAA CCTCGCTGCC ACCTTACCTA CAGTACGAAT GATTGTAGAT 
GTTAGCAAAG CTGCCTGCAT TCTCACTACA CAAGCATTGC TGAGATTGCT CAAGTCCAAA 
GAAGCAGCAG CGGCTGTGGA TGTGAAAACT TGGCCAACCA TCATCGACAC CGATGATTTG 
CCCAGGAAAC GGGTACCGCA GCTGTATAAA CCACCTACAC CTGAAATGCT GGCTTACCTG 
GACTTCAGTG TGAGCACGAC TGGCATGCTT ACGGGGGTTA AGATGTCTCA TGGATCAGTA 
AATGCACTGT GCCGGTCCAT AAAGCTGCAG TGTGAGCTGT ACTCTACTCG TCAGATCGCC 
ATTTGTCTTG ATCCTTATTG TGGGCTTGGC TTTGTACTTT GGTGCCTTTG TAGTGTTTAT 
TCTGGTCATC AGTCTATATT AATTCCTCCT ATGGAGTTGG AATCCAACTT ATTCTTGTGG 
CTCTCTGCTG TGAGTCAGTA TAAAATTAGG GACACGTTCT GTTCATATTC TGTGATGGAG 
ATGTGCTCAA AGGGACTGGG GAACCAGATT GAGGTGCTAA AGACCAAAGG CATTAATCTG 
TCTTACGTGC GGACCTGTGT GGTGGTTGCT GAAGAGCGGC CGAGGGTGGC TTTGGCATAT 
TCCTTTTCAA AACTCTTCAA AGACCTTGGG CTGTCCCCCC GCGCTGTGAG CACAACCTTT 
GGATCCAGAG TTAACACCGC TATATGCTTG CAGGGAACCT CAGGGCCAGA TCCAACAACT 
GTGTATGTGG ATCTGAAATC CCTTCGGCAT GACAGGGTGC GCCTGGTGGA GAGGGGATCT 
CCGCAGAGTT TGCTCCTATT GGAATCTGGA AAGATTTTAC CTGGTGTCAC CGTAATTATT 
GTTAATCCAG AGACCAAAGG ACCATTGGGG GATTCCCACC TTGGAGAGAT CTGGGTGAAC 
AGCGCCCACA CAGCCAGCGG ATACTACACA ATATATGATA GTGAGACCTT ACAGGCTGAT 
CATTTCAATA CTAGGCTTAG CTTTGGAGAC ACACAGACAC TTTGGGCCAA AACGGGCTAT 
CTTGGCTTTG TCCGCAGGAC AGAACTGACT GATGCAAATG GAGAGCGGCA CGATGCGCTG 
TTTGTGGTTG GAGCACTAGA TGAAACGCTG GAGCTGAGGG GGCTTCGTTA TCATCCGATA 
GATATCGAGA CCTCAGTGTC ACGCATACAC CGGAGTATCG CTGAATGTGC TGTTTTCACA 
TGGACCAACC TGCTGGTAGT GGTGGTGGAG CTGTGCGGCT CAGAACAGGA GGCTCTGGAC 
TTGGTTCCTC TGGTGACCAA CGTGGTGTTG GAAGAACATT ACCTGATTGT GGGCGTGGTG 
GTGGTGGTGG ATCCCGGCGT GATACCCATC AATTCCCGGG GAGAGAAACA ACGGATGCAC 
CTGCGGGACA GTTTCTTAGC GGACCAGTTG GATCCAATCT ACGTGGCGTA CAACATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dip2b ( XM_004911909.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911966.1 Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X2, mRNA.

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CloneID	OXa06063
Clone ID Related Accession (Same CDS sequence)	XM_004911909.3
Accession Version	XM_004911909.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	disco-interacting protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGA GAGCCTTCGA CCTATCCGCC CTCCCGAAGG AGGTGAGGGA GCAGCTGGCA 
GAACTGGAGC TGGAGCTGTC AGAAGGTGAT ATCACCCAGA AGGGTTATGA GAAAAAAAGA 
GCAAAACTAT TGGGTCCGTA CATCCCGCAA ACACAAGGTG TTGATCCAGC GCTACAGAAA 
GAGAATCGGA ACCAAACAAC TGGATCACCT GCTGCTCCTG CGCAATCGTC CTCTAAGTAC 
CATCGAGCCA GATCCGGAGG AGCTAGAGAT GAGCGTTACA GATCAGATAT CCATACAGAA 
GCAGTCCAAG CGGCTCTGGC TAAGCATAAA GAACAGAAGA TGGCATTACC AATGCCAACC 
AAAAGGCGGT CAACATTTGT GCAGTCTCCA GCTGACGCAT GTACCCCTCC AGATACATCC 
TCCGCATCTG AGGACGAAGG ATCCCTAAGA CGCCAGGCTG CTCTTTCGGC AGCTCTTGCT 
CAGAGTTTAC AGAATTCAGA GGCCTGGATC AACAGGTCCG TTCAGGGTTC ATCCACATCA 
TCTTCAGCAT CCTCCACATT ATCGCACGGA GAAGTAAAGA GTACCAGTGG CACCTTAGCA 
GATATACTTT CTAATACTCA CATAGACAGT TCTCCTGCTC CACCCGATGT TACCAACACC 
ACCAATTCCA CAACCACTAC AAGCACATCA TCGTCTTCGT CATCCTTGTC TTCAGTTCGC 
CCAGCAACCA TTGACCTTCC CCCTGCAGGC CTGGTGAAAG GGGTGCACAA GGCAGCTAGA 
AGGTCTAGCT TAATGGATAC TGCAGATGGT GTTGCAGTGA ACAGTCGCGT CTCAACAAAA 
ATCCAGCAGT TGCTGAACAC GCTCAAGCGA CCAAAAAGGC CACCATTAAA GGAATTTTTT 
GTGGATGATT TTGAAGAGAT TGTAGAAGTA CCCCGACCAG ACCCAAACCA GCCCAAACCA 
GAAGGTCGCC AGGTGACCCC TGTAAAAGGA GAGCCCCTGG GTGTTGTGTG CAACTGGCCC 
CCGTCGGTAG AAGCCGCACT GCAGCGCTGG GGAACAACAC AAGCAAAATG TTCATGTCTT 
ACCGCTCTTG ATGTCACTGG CAAACCAACC TATACGTTAA CATATGGCAA GCTATGGACC 
AGGAGTGTCA GACTGGCTTA TACCCTGCTG AACAAATTGG GTACCAAGAA TGAACAAGTG 
CTGAAGCCTG GGGACAGGGT TGCCCTGGTT TACCCCAATA ATGACCCAGT GATGTTCATG 
GTTGCGTTTT ATGGCTGTCT CTTGGCGGAG GTGATCCCAG TGCCGATTGA GGACGCTGGA 
GGGCATCAGA TTGGATTCCT GCTGGGAAGC TGTGGTGTTA AGATCGCTCT TACTACTGAG 
GTTTGCTTGA AGGGGCTGCC CAAGACACAG AACGGGGAGA TTGTGCAATT CAAAGGTTGG 
CCAAAACTCA AGTGGGTTGT AACAGACTCC AGATACCTAT CAAAATCACC AAAGGACTGG 
CAGCCACACA TCACCAATGC TGGCACAGAG CCGGCATACA TTGAGTACAA AACAAGCAAG 
GAAGGCAGTG TGATGGGTGT GACCGTGTCT CGGATAGCCA TGCTGTCTCA TTGCCAGGCA 
CTCTCACAGG CCTGTAACTA CACAGAGGGT GAGACCTTAG TTAATGTCCT GGACTTCAAG 
AGGGATGCAG GCCTGTGGCA TGGTATACTA ACGAATGTAG TAAATAAAAT GCACACGATC 
AGCGTGCCGT ACGCAGTGAT GAAAGCATGT CCGTTGTCCT GGGTGCAGAG GGTACATGCC 
AGTAAAGGCA GGGTGGCTGT AGTGAAGTGC CGGGACTTGC ACTGGGCCAT GATAGCCCAC 
CGAGAACAAA AGGATATCAG TCTGGCCTCT CTCCGTATGC TCATTGTTAC AGATGGAGCT 
AATCCTTGGT CCGTTTCCTC ATGTGATGCA TTCCTGAGTT TGTTTCAGAA TCACGGCCTG 
AAGCCTGAGG TCATCTGTCC ATGTGCAACC TCTCCAGAAG CTATGACTGT CGCTATTCGA 
AGGCCTGGAG TACCCGGTGC CCCCCTGCCT GGAAGAGCCA TTTTGTCTAT GAATGGGTTA 
AGTTATGGCG TGATCAGAGT TAATACAGAG GACAAGAATT CTGCATTGAC CGTCCAGGAC 
GTTGGACATG TTATGCCAGG AAGTGTGATG TGCACTGTGA AGCCGGAAGG AGTACCCCAG 
CTCTGTAAAA CTGATGAGAT AGGAGAGATC TGTGTGAGCT GTAAAGGAGG AGGCACAATG 
TACTATGGCC TGGCAGGGCT CACCAAGAAC ACCTTTGAGG TTTTGCCAGT TAATGCTTCT 
GGGGCTTCTA TAGGAGAAAT ACCCTTCATC AGAACTGGGC TACTTGGATT TGTGGGGCCA 
GGCAGTTTAG TGTTTGTGGT GGGAAAGATG GACGGCACGG TGATGGTGAG TGGGAGGCGC 
CACAATGCTG ATGATATTGT GGCTACTGCC CTGGCGGTGG AATCCTTTAA AACCCTGTAC 
AGAGGAAGGA TTGCTGTGTT TTCAGTCACA GTATTTTATG ACGAGCGGAT TGTGGTGGTG 
GCAGAACAGC GCCCTGATGC TTCTGAGGAA GATTCCTTCC AATGGATGAG CCGTGTATTA 
CAGGCCATTG ATAGTATCCA CCAAGTGGGA GTTTACTGTC TTGCCCTGGT TCCAGCCAAT 
ACGTTGCCAA AAACTCCACT GGGAGGAATC CACATATCGC AAACTAAACA GCTTTTCTTG 
GAGGGCAGCT TACACCCCTG TAACATACTC ATGTGTCCTC ATACGTGTGT AACCAACTTG 
CCAAAACCAA GGCAGAAGCA GCCAGGAGTT GGGCCAGCAT CTGTCATGGT GGGTAATCTG 
GTCGCAGGAA AAAGAATTGC TCAGGCCTCC GGAAGAGATT TGGGACAAAT AGAGGAAAAT 
GATTTGGTGA AAAAGCACCA GTATCTGACG GAAGTCTTAC AGTGGCGAGC GCAAACCACT 
CCCGAACACG TTCTTTTTAT CCTCCTCAAT GCAAAGGGAA CGGCAGTTTG CAATGCCTCA 
TGTTTACAGC TACACAAGCG AGCAGAAAGA ATAGCAGCTG CCCTATGTGA TAAAGGTCAT 
CTCAACGCCG GCGATAACGT GGTGCTACTT TTTCCTCCCG GAATTGAGTT GATAGCTGCA 
TTCTATGGCT GCCTGTACGC TGGCTGTATT CCTGTAACTG TCCGGCCGCC TCACGCACAG 
AACCTCGCTG CCACCTTACC TACAGTACGA ATGATTGTAG ATGTTAGCAA AGCTGCCTGC 
ATTCTCACTA CACAAGCATT GCTGAGATTG CTCAAGTCCA AAGAAGCAGC AGCGGCTGTG 
GATGTGAAAA CTTGGCCAAC CATCATCGAC ACCGATGATT TGCCCAGGAA ACGGGTACCG 
CAGCTGTATA AACCACCTAC ACCTGAAATG CTGGCTTACC TGGACTTCAG TGTGAGCACG 
ACTGGCATGC TTACGGGGGT TAAGATGTCT CATGGATCAG TAAATGCACT GTGCCGGTCC 
ATAAAGCTGC AGTGTGAGCT GTACTCTACT CGTCAGATCG CCATTTGTCT TGATCCTTAT 
TGTGGGCTTG GCTTTGTACT TTGGTGCCTT TGTAGTGTTT ATTCTGGTCA TCAGTCTATA 
TTAATTCCTC CTATGGAGTT GGAATCCAAC TTATTCTTGT GGCTCTCTGC TGTGAGTCAG 
TATAAAATTA GGGACACGTT CTGTTCATAT TCTGTGATGG AGATGTGCTC AAAGGGACTG 
GGGAACCAGA TTGAGGTGCT AAAGACCAAA GGCATTAATC TGTCTTACGT GCGGACCTGT 
GTGGTGGTTG CTGAAGAGCG GCCGAGGGTG GCTTTGGCAT ATTCCTTTTC AAAACTCTTC 
AAAGACCTTG GGCTGTCCCC CCGCGCTGTG AGCACAACCT TTGGATCCAG AGTTAACACC 
GCTATATGCT TGCAGGGAAC CTCAGGGCCA GATCCAACAA CTGTGTATGT GGATCTGAAA 
TCCCTTCGGC ATGACAGGGT GCGCCTGGTG GAGAGGGGAT CTCCGCAGAG TTTGCTCCTA 
TTGGAATCTG GAAAGATTTT ACCTGGTGTC ACCGTAATTA TTGTTAATCC AGAGACCAAA 
GGACCATTGG GGGATTCCCA CCTTGGAGAG ATCTGGGTGA ACAGCGCCCA CACAGCCAGC 
GGATACTACA CAATATATGA TAGTGAGACC TTACAGGCTG ATCATTTCAA TACTAGGCTT 
AGCTTTGGAG ACACACAGAC ACTTTGGGCC AAAACGGGCT ATCTTGGCTT TGTCCGCAGG 
ACAGAACTGA CTGATGCAAA TGGAGAGCGG CACGATGCGC TGTTTGTGGT TGGAGCACTA 
GATGAAACGC TGGAGCTGAG GGGGCTTCGT TATCATCCGA TAGATATCGA GACCTCAGTG 
TCACGCATAC ACCGGAGTAT CGCTGAATGT GCTGTTTTCA CATGGACCAA CCTGCTGGTA 
GTGGTGGTGG AGCTGTGCGG CTCAGAACAG GAGGCTCTGG ACTTGGTTCC TCTGGTGACC 
AACGTGGTGT TGGAAGAACA TTACCTGATT GTGGGCGTGG TGGTGGTGGT GGATCCCGGC 
GTGATACCCA TCAATTCCCG GGGAGAGAAA CAACGGATGC ACCTGCGGGA CAGTTTCTTA 
GCGGACCAGT TGGATCCAAT CTACGTGGCG TACAACATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911966.1
CDS	204..4925
Translation	MAERAFDLSALPKEVREQLAELELELSEGDITQKGYEKKRAKLLGPYIPQTQGVDPALQKENRNQTTGSPAAPAQSSSKYHRARSGGARDERYRSDIHTEAVQAALAKHKEQKMALPMPTKRRSTFVQSPADACTPPDTSSASEDEGSLRRQAALSAALAQSLQNSEAWINRSVQGSSTSSSASSTLSHGEVKSTSGTLADILSNTHIDSSPAPPDVTNTTNSTTTTSTSSSSSSLSSVRPATIDLPPAGLVKGVHKAARRSSLMDTADGVAVNSRVSTKIQQLLNTLKRPKRPPLKEFFVDDFEEIVEVPRPDPNQPKPEGRQVTPVKGEPLGVVCNWPPSVEAALQRWGTTQAKCSCLTALDVTGKPTYTLTYGKLWTRSVRLAYTLLNKLGTKNEQVLKPGDRVALVYPNNDPVMFMVAFYGCLLAEVIPVPIEDAGGHQIGFLLGSCGVKIALTTEVCLKGLPKTQNGEIVQFKGWPKLKWVVTDSRYLSKSPKDWQPHITNAGTEPAYIEYKTSKEGSVMGVTVSRIAMLSHCQALSQACNYTEGETLVNVLDFKRDAGLWHGILTNVVNKMHTISVPYAVMKACPLSWVQRVHASKGRVAVVKCRDLHWAMIAHREQKDISLASLRMLIVTDGANPWSVSSCDAFLSLFQNHGLKPEVICPCATSPEAMTVAIRRPGVPGAPLPGRAILSMNGLSYGVIRVNTEDKNSALTVQDVGHVMPGSVMCTVKPEGVPQLCKTDEIGEICVSCKGGGTMYYGLAGLTKNTFEVLPVNASGASIGEIPFIRTGLLGFVGPGSLVFVVGKMDGTVMVSGRRHNADDIVATALAVESFKTLYRGRIAVFSVTVFYDERIVVVAEQRPDASEEDSFQWMSRVLQAIDSIHQVGVYCLALVPANTLPKTPLGGIHISQTKQLFLEGSLHPCNILMCPHTCVTNLPKPRQKQPGVGPASVMVGNLVAGKRIAQASGRDLGQIEENDLVKKHQYLTEVLQWRAQTTPEHVLFILLNAKGTAVCNASCLQLHKRAERIAAALCDKGHLNAGDNVVLLFPPGIELIAAFYGCLYAGCIPVTVRPPHAQNLAATLPTVRMIVDVSKAACILTTQALLRLLKSKEAAAAVDVKTWPTIIDTDDLPRKRVPQLYKPPTPEMLAYLDFSVSTTGMLTGVKMSHGSVNALCRSIKLQCELYSTRQIAICLDPYCGLGFVLWCLCSVYSGHQSILIPPMELESNLFLWLSAVSQYKIRDTFCSYSVMEMCSKGLGNQIEVLKTKGINLSYVRTCVVVAEERPRVALAYSFSKLFKDLGLSPRAVSTTFGSRVNTAICLQGTSGPDPTTVYVDLKSLRHDRVRLVERGSPQSLLLLESGKILPGVTVIIVNPETKGPLGDSHLGEIWVNSAHTASGYYTIYDSETLQADHFNTRLSFGDTQTLWAKTGYLGFVRRTELTDANGERHDALFVVGALDETLELRGLRYHPIDIETSVSRIHRSIAECAVFTWTNLLVVVVELCGSEQEALDLVPLVTNVVLEEHYLIVGVVVVVDPGVIPINSRGEKQRMHLRDSFLADQLDPIYVAYNM

Target ORF information:

RefSeq Version	XM_004911909.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis disco interacting protein 2 homolog B (dip2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911909.3

ORF Insert Sequence:

ATGGCGGAGA GAGCCTTCGA CCTATCCGCC CTCCCGAAGG AGGTGAGGGA GCAGCTGGCA 
GAACTGGAGC TGGAGCTGTC AGAAGGTGAT ATCACCCAGA AGGGTTATGA GAAAAAAAGA 
GCAAAACTAT TGGGTCCGTA CATCCCGCAA ACACAAGGTG TTGATCCAGC GCTACAGAAA 
GAGAATCGGA ACCAAACAAC TGGATCACCT GCTGCTCCTG CGCAATCGTC CTCTAAGTAC 
CATCGAGCCA GATCCGGAGG AGCTAGAGAT GAGCGTTACA GATCAGATAT CCATACAGAA 
GCAGTCCAAG CGGCTCTGGC TAAGCATAAA GAACAGAAGA TGGCATTACC AATGCCAACC 
AAAAGGCGGT CAACATTTGT GCAGTCTCCA GCTGACGCAT GTACCCCTCC AGATACATCC 
TCCGCATCTG AGGACGAAGG ATCCCTAAGA CGCCAGGCTG CTCTTTCGGC AGCTCTTGCT 
CAGAGTTTAC AGAATTCAGA GGCCTGGATC AACAGGTCCG TTCAGGGTTC ATCCACATCA 
TCTTCAGCAT CCTCCACATT ATCGCACGGA GAAGTAAAGA GTACCAGTGG CACCTTAGCA 
GATATACTTT CTAATACTCA CATAGACAGT TCTCCTGCTC CACCCGATGT TACCAACACC 
ACCAATTCCA CAACCACTAC AAGCACATCA TCGTCTTCGT CATCCTTGTC TTCAGTTCGC 
CCAGCAACCA TTGACCTTCC CCCTGCAGGC CTGGTGAAAG GGGTGCACAA GGCAGCTAGA 
AGGTCTAGCT TAATGGATAC TGCAGATGGT GTTGCAGTGA ACAGTCGCGT CTCAACAAAA 
ATCCAGCAGT TGCTGAACAC GCTCAAGCGA CCAAAAAGGC CACCATTAAA GGAATTTTTT 
GTGGATGATT TTGAAGAGAT TGTAGAAGTA CCCCGACCAG ACCCAAACCA GCCCAAACCA 
GAAGGTCGCC AGGTGACCCC TGTAAAAGGA GAGCCCCTGG GTGTTGTGTG CAACTGGCCC 
CCGTCGGTAG AAGCCGCACT GCAGCGCTGG GGAACAACAC AAGCAAAATG TTCATGTCTT 
ACCGCTCTTG ATGTCACTGG CAAACCAACC TATACGTTAA CATATGGCAA GCTATGGACC 
AGGAGTGTCA GACTGGCTTA TACCCTGCTG AACAAATTGG GTACCAAGAA TGAACAAGTG 
CTGAAGCCTG GGGACAGGGT TGCCCTGGTT TACCCCAATA ATGACCCAGT GATGTTCATG 
GTTGCGTTTT ATGGCTGTCT CTTGGCGGAG GTGATCCCAG TGCCGATTGA GGACGCTGGA 
GGGCATCAGA TTGGATTCCT GCTGGGAAGC TGTGGTGTTA AGATCGCTCT TACTACTGAG 
GTTTGCTTGA AGGGGCTGCC CAAGACACAG AACGGGGAGA TTGTGCAATT CAAAGGTTGG 
CCAAAACTCA AGTGGGTTGT AACAGACTCC AGATACCTAT CAAAATCACC AAAGGACTGG 
CAGCCACACA TCACCAATGC TGGCACAGAG CCGGCATACA TTGAGTACAA AACAAGCAAG 
GAAGGCAGTG TGATGGGTGT GACCGTGTCT CGGATAGCCA TGCTGTCTCA TTGCCAGGCA 
CTCTCACAGG CCTGTAACTA CACAGAGGGT GAGACCTTAG TTAATGTCCT GGACTTCAAG 
AGGGATGCAG GCCTGTGGCA TGGTATACTA ACGAATGTAG TAAATAAAAT GCACACGATC 
AGCGTGCCGT ACGCAGTGAT GAAAGCATGT CCGTTGTCCT GGGTGCAGAG GGTACATGCC 
AGTAAAGGCA GGGTGGCTGT AGTGAAGTGC CGGGACTTGC ACTGGGCCAT GATAGCCCAC 
CGAGAACAAA AGGATATCAG TCTGGCCTCT CTCCGTATGC TCATTGTTAC AGATGGAGCT 
AATCCTTGGT CCGTTTCCTC ATGTGATGCA TTCCTGAGTT TGTTTCAGAA TCACGGCCTG 
AAGCCTGAGG TCATCTGTCC ATGTGCAACC TCTCCAGAAG CTATGACTGT CGCTATTCGA 
AGGCCTGGAG TACCCGGTGC CCCCCTGCCT GGAAGAGCCA TTTTGTCTAT GAATGGGTTA 
AGTTATGGCG TGATCAGAGT TAATACAGAG GACAAGAATT CTGCATTGAC CGTCCAGGAC 
GTTGGACATG TTATGCCAGG AAGTGTGATG TGCACTGTGA AGCCGGAAGG AGTACCCCAG 
CTCTGTAAAA CTGATGAGAT AGGAGAGATC TGTGTGAGCT GTAAAGGAGG AGGCACAATG 
TACTATGGCC TGGCAGGGCT CACCAAGAAC ACCTTTGAGG TTTTGCCAGT TAATGCTTCT 
GGGGCTTCTA TAGGAGAAAT ACCCTTCATC AGAACTGGGC TACTTGGATT TGTGGGGCCA 
GGCAGTTTAG TGTTTGTGGT GGGAAAGATG GACGGCACGG TGATGGTGAG TGGGAGGCGC 
CACAATGCTG ATGATATTGT GGCTACTGCC CTGGCGGTGG AATCCTTTAA AACCCTGTAC 
AGAGGAAGGA TTGCTGTGTT TTCAGTCACA GTATTTTATG ACGAGCGGAT TGTGGTGGTG 
GCAGAACAGC GCCCTGATGC TTCTGAGGAA GATTCCTTCC AATGGATGAG CCGTGTATTA 
CAGGCCATTG ATAGTATCCA CCAAGTGGGA GTTTACTGTC TTGCCCTGGT TCCAGCCAAT 
ACGTTGCCAA AAACTCCACT GGGAGGAATC CACATATCGC AAACTAAACA GCTTTTCTTG 
GAGGGCAGCT TACACCCCTG TAACATACTC ATGTGTCCTC ATACGTGTGT AACCAACTTG 
CCAAAACCAA GGCAGAAGCA GCCAGGAGTT GGGCCAGCAT CTGTCATGGT GGGTAATCTG 
GTCGCAGGAA AAAGAATTGC TCAGGCCTCC GGAAGAGATT TGGGACAAAT AGAGGAAAAT 
GATTTGGTGA AAAAGCACCA GTATCTGACG GAAGTCTTAC AGTGGCGAGC GCAAACCACT 
CCCGAACACG TTCTTTTTAT CCTCCTCAAT GCAAAGGGAA CGGCAGTTTG CAATGCCTCA 
TGTTTACAGC TACACAAGCG AGCAGAAAGA ATAGCAGCTG CCCTATGTGA TAAAGGTCAT 
CTCAACGCCG GCGATAACGT GGTGCTACTT TTTCCTCCCG GAATTGAGTT GATAGCTGCA 
TTCTATGGCT GCCTGTACGC TGGCTGTATT CCTGTAACTG TCCGGCCGCC TCACGCACAG 
AACCTCGCTG CCACCTTACC TACAGTACGA ATGATTGTAG ATGTTAGCAA AGCTGCCTGC 
ATTCTCACTA CACAAGCATT GCTGAGATTG CTCAAGTCCA AAGAAGCAGC AGCGGCTGTG 
GATGTGAAAA CTTGGCCAAC CATCATCGAC ACCGATGATT TGCCCAGGAA ACGGGTACCG 
CAGCTGTATA AACCACCTAC ACCTGAAATG CTGGCTTACC TGGACTTCAG TGTGAGCACG 
ACTGGCATGC TTACGGGGGT TAAGATGTCT CATGGATCAG TAAATGCACT GTGCCGGTCC 
ATAAAGCTGC AGTGTGAGCT GTACTCTACT CGTCAGATCG CCATTTGTCT TGATCCTTAT 
TGTGGGCTTG GCTTTGTACT TTGGTGCCTT TGTAGTGTTT ATTCTGGTCA TCAGTCTATA 
TTAATTCCTC CTATGGAGTT GGAATCCAAC TTATTCTTGT GGCTCTCTGC TGTGAGTCAG 
TATAAAATTA GGGACACGTT CTGTTCATAT TCTGTGATGG AGATGTGCTC AAAGGGACTG 
GGGAACCAGA TTGAGGTGCT AAAGACCAAA GGCATTAATC TGTCTTACGT GCGGACCTGT 
GTGGTGGTTG CTGAAGAGCG GCCGAGGGTG GCTTTGGCAT ATTCCTTTTC AAAACTCTTC 
AAAGACCTTG GGCTGTCCCC CCGCGCTGTG AGCACAACCT TTGGATCCAG AGTTAACACC 
GCTATATGCT TGCAGGGAAC CTCAGGGCCA GATCCAACAA CTGTGTATGT GGATCTGAAA 
TCCCTTCGGC ATGACAGGGT GCGCCTGGTG GAGAGGGGAT CTCCGCAGAG TTTGCTCCTA 
TTGGAATCTG GAAAGATTTT ACCTGGTGTC ACCGTAATTA TTGTTAATCC AGAGACCAAA 
GGACCATTGG GGGATTCCCA CCTTGGAGAG ATCTGGGTGA ACAGCGCCCA CACAGCCAGC 
GGATACTACA CAATATATGA TAGTGAGACC TTACAGGCTG ATCATTTCAA TACTAGGCTT 
AGCTTTGGAG ACACACAGAC ACTTTGGGCC AAAACGGGCT ATCTTGGCTT TGTCCGCAGG 
ACAGAACTGA CTGATGCAAA TGGAGAGCGG CACGATGCGC TGTTTGTGGT TGGAGCACTA 
GATGAAACGC TGGAGCTGAG GGGGCTTCGT TATCATCCGA TAGATATCGA GACCTCAGTG 
TCACGCATAC ACCGGAGTAT CGCTGAATGT GCTGTTTTCA CATGGACCAA CCTGCTGGTA 
GTGGTGGTGG AGCTGTGCGG CTCAGAACAG GAGGCTCTGG ACTTGGTTCC TCTGGTGACC 
AACGTGGTGT TGGAAGAACA TTACCTGATT GTGGGCGTGG TGGTGGTGGT GGATCCCGGC 
GTGATACCCA TCAATTCCCG GGGAGAGAAA CAACGGATGC ACCTGCGGGA CAGTTTCTTA 
GCGGACCAGT TGGATCCAAT CTACGTGGCG TACAACATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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dip2b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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