crb2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following crb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa17941 XM_002937234.5
Latest version!
Xenopus tropicalis crumbs 2, cell polarity complex component (crb2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa17942 XM_004918924.4
Latest version!
Xenopus tropicalis crumbs 2, cell polarity complex component (crb2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa64134 XM_031890901.1
Latest version!
Xenopus tropicalis crumbs 2, cell polarity complex component (crb2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
View Old Accession Versions >>
OXa17942 XM_004918924.3 Xenopus tropicalis crumbs family member 2 (crb2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa17941 XM_002937234.4 Xenopus tropicalis crumbs family member 2 (crb2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa17941
    Clone ID Related Accession (Same CDS sequence) XM_002937234.5 , XM_002937234.4
    Accession Version XM_002937234.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein crumbs homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937234.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTACA GAAGCGTTAG TCGCACTTCA CCCAGTCCCG TTATTCTGCT ACTGCTACTT 
    CTCTTCTCCT GCGGGCAAGG ACTTGCCCAG ACCTGTGTGT CGTCCCCTTG CCTGAATGGT
    GGAACATGCC AGGAAACAGC AAGTGGATTT ACCTGTCTGT GCCCAGCGGA GCCATTGGCA
    TTTACTGGAA CTACCTGTGG AGATCTGTAT GATGCCTGTG CCATTGATAG ATGCCCCAGC
    ACCCATAGCT GTCAGAGCAC CCCAGGGCAT CCTCATTTTC AGTGTGTTTG CCAACCTGGG
    TTGAACTGCA CCAATGAGTG TCAGAGAAAT TCCTGCCCAT TCCAAAATTT TCAGTGTACA
    GTTGGTGAGG ACAGCTTTAT GTGCAAGTGC CAAAGTGGGT ACGGTGGAGT GGACTGCAAC
    TTTGAGGTAT CACCGTGTTT TCAGAACCCC TGCCAGAACA ATGCCACCTG TTTGGCAGAA
    GGAGACAGCT TCAGGTGTGT ATGTGAGCCA GGGTTCACAG GGCAGGAATG TGAGGTCAAT
    GTGAACGAAT GTGCTTCAAA CCCATGCCAG AACGAGGCTC TCTGTGTGGA TAAAGTGGAC
    AGATACATGT GCTTCTGTGT GCCCGGCTTC CAAGGCCACC ATTGCGAAAT AGACATCAAC
    GAGTGTGCTT CCAGACCATG TCAGAACAAC GGAACTTGTA TGAACCAACT GGACCAATAT
    GAATGTGCTT GTGCCAATGG TTACACAGGA ATTAACTGTG AGATGGAGAT AGATGAATGT
    CAGTCAGTTC CATGTCAGAA TGGAGCTACA TGTAGGGACC ATATTGGCTA CTTCACGTGT
    GACTGCCCAG CGGGCTATGA TGGAGAACAG TGCCAGCATG ACATTGATGA GTGCCAGAGC
    CAGCCCTGTC AGAATGGAGG GCAGTGTATG GATGAAATTA ACAGGTTCCG CTGTGATTGC
    AGTAATACTG GCTTTGTTGG TGGCCGCTGT GAAGTAGAAA TCCTGGAGTG TGACTCAAAT
    CCTTGTCAGA ACAATGGCAC GTGCATGGAG AGAGTCAATG GCTACGAATG CTTGTGCTGG
    AAAGGTTATT CTGGAATTCA TTGTGAAATA GATGATAATG AATGTTTTGA GGAGCCATGT
    GTAAATGGTG GCCTATGTCT TGAACTATCC AACCAGTCAT ATTATGGCAC TGAACCAGAA
    TTTAACAAGT TCAGCTACAG CCAAGCAGCA GGATATTTGT GCCGCTGCCA GCAAGGATTT
    ACAGGACCAA ACTGCTCTGT GAACATTGAT GAATGTGAAT CTTGGCCTTG TCTGAATGGC
    GGAACCTGCA CCGACTTAAT CAATGGCTTC CTGTGTAACT GTGCTTCTGG ATATACAGGT
    TTCGACTGTT CCATAAACAT CGATGAGTGT AAAGACAGCC CATGTAAGAA TGGGGCAAGC
    TGTGAAGATG GAGTTGCTGA CTACTCCTGC ATCTGTCCAC CAGCTGCCCA GGATGGGACA
    GTATGGGGTG GTAAAAACTG TTCTGTAATG TTGATTGGAT GCTTGGAGCA CAGCTGCCAA
    AACGCAGCAG AGTGCATCCC TTTATATACT GGACAAAGCC ACAGTTATAC CTGCAAATGT
    CAACCAGGTT TTGTTGGGAA CAATTGTTCA ACCCCTACAA CATTTTCCTT CTTGTCCCAG
    GGGTACCTAA TGTATGATTC ACCTTTAAGT AATGAATCGT ATCAAAGCAA CATGTCAGTG
    AGATTCAGGA CAACATTACC TGACATGGTC CTCATGTACC GAGGAGATGA AGATAGCTTT
    CTCGCACTTG AGATTTATAA TGGATTTCTG CACATGCGCC TTAAGGGAAA TGGAAGGTTG
    TTTCCGCTGG TTATTGATGA TTACAAGGTG AACGATGGCC ACTGGCACAA TGTGGAGGTA
    GACCTTCTAT CAAATTCATT CTTGAAGATC AGCCTTTTTC ATAATGATTG CCGTAATGGA
    CTCTGCTCCC AAAAACAGAC ACCCACTGAA GGATTTGACC CCTATCTTCC AGAGTCCTTC
    AGATCATTGT ATATTGGAGG ACTTATTCAA GAGTCATTAA TCAAGAACAC TCTAAGTCAG
    CAAAACTTCA CTGGCTGCCT GGAGGACTTG GTTGTTGACT TGGTTACTCA GCTGCCGCAC
    AACGTAAGCA GTGACAGGTC CTTTGGAATG GTACTCGGAT GCAAAAAGGC GGAGGTGTGC
    CATCCAAACC CATGTCACAA TGGGGCAAAC TGCATTGATC TCTGGTCCAG CTTTACATGT
    GAATGCATCA GGCCCTTTAA AGGGTCCACC TGTATGCAGG AGTATATAGC AGGAACATTC
    TTCAGGGAGG GGATTCCAAG CCACTCTTAC TTTGAACTCA ATCAGGATTT CGGAACGTCT
    TTCAATATAT CTGCTTTTAT CAGAACCCTA AAATCGAATG GTCTCCTACT GCAAATCAGC
    TACGGAACAA CGCCTTACTT CAGAACTTAC CTGAATAATA GTTGGATCCA CATTGAGACA
    CTATCTGCAC AACCTCTTGT CTTTAAGAAG AACGTCAGTG ATGGCATGAA GCACTTTATA
    AGTATTTCTG TCAGGGAAGG TAACGTTACA GTCACTGATC TTCAGCAGGA AGTTTACTTG
    GGAATGTTGC CGCCGGTGAT CATTACCAAA GGAGATGCAG TCCATGTGGG CGGTTTGGCT
    ACAGCAGACA GTACTATTTT TTGGGGTGGG TATTTCAAAG GATGTGTTCA AGATTTAAGG
    ATTAACAACG ATCACTTGGA GTTCTTCCAC CCAGAACATA TAAATGTACA AGAAACCTTA
    TACTTTGGGA ACAACACCAA CGTCACTGAG AACTGCATCT CTGATGATGT TTGTCATCAA
    GGCCCATGCA GAAATGAAGG AAACTGCTCA GTAACCTGGA ATGACTTTGT TTGCAGCTGT
    CCGGCCAACT TCACCGGAAA AAGATGTGAG GAGCCGGTTT GGTGTCGACG CTCTCCCTGC
    TTGCCAGGCT CCACTTGCGT GGATGTTCCC AAAGGATTTG TGTGTCTGGC CAATGTGAGC
    TTCCAGGGCC ACAGCTCTGC AGTATTCACT CCCATTATTT CAGAAGAACA CAATCTTACA
    TCTATCTCAT TGGCCTTCAG AACCAGAGAC AGAGATGCAG TTTTGCTCCA GTCTTCCAAA
    AATGTGGATT CTGTGCTAAT AGAAATTCAG GGGGGTTACT TGTTTGTTGA TCTGCAAAGT
    GGAAATAATG TGGATGGAGT CCAGCTTAAT GGAACAATAG ATGTTGCTGA TGCTATCTGG
    CACAGAGTAG TAATAACCAT GGAAAGAGCG ACTAAAGTGA ATTCTCATTG GACATTCCAG
    CTGGATGACA GTGTTCAAAA GAAATTTCAG TGGGAAGGCG ACAACTTGAA TTTTATGAGA
    AATAACATTT CCATTGTATT AGCTGAAGGC TACACTGGAT GTTTGGGCGA TGTGTCAATT
    GGAGGGATTT ACCTTCCATT TACAGACCAT GTGTTGCCAC AGCAGGATCA GTTTATAAGG
    AAATCTGATG CTTTGGAGTT GGGATGCAGA GGGGCAGATG TTTGTTCTGA GAGTCCCTGT
    CTTCATGGTG GAACATGCCA AGATCTTTTT AACTCCTTTA GCTGTACGTG CAGAATAGGG
    TGGAATGGGC ATCGTTGTGA GATCAACATC GATGACTGCA AGTCTAGCCC ATGTCTTCAT
    GGTTTTTGTA TAGACCTAGA GGCTGATTAT CAGTGCAGCT GTGAGGCTGG TTATACCAGC
    AGGAACTGCG AAATCAACAT CAATGACTGT CAGCACCATC AGTGCCTGAA TGGGGGGACT
    TGTGTTGATG GCGTAAATAA CTACACATGC AAGTGCCCAG CCAACTATGC GGGGACCTTC
    TGCCAATGGC CTTTTCCACC TGAGCAGTGC AATAAAAACA TCACGTGTTT TAATGGAGGC
    AGATGCAACA GTGGAATTTG GGGAGCTAAC TGCACATGTA GACCAGGCTT TAGTGGCAGA
    AGGTGTGAAA TAAATGTCAA CGATTGTGAA TCAAACCCAT GTCTCAATGG TGGAACTTGC
    CAGGATTCTG TAAATACCTT CAAATGCATT TGCAATTCCA GCTTTAGTGG GGTGCGCTGT
    GAGAAAAGCG ATGCAGCCTC AATGTTCCCC TTTCCTCTTG TTGGAGTTGC AGTACCTGTA
    ACCTGTGGGG TTTTACTTCT ACTTGTTATT GGGGTCATAT TCATGGTGCT GACAGCAAGG
    AAGAGGAGAC AGTCAGAAGG AACCTACAGC CCAAGCCAAC AAGAAGTTGC AGGAGCACGA
    CTTGAAATGG ACAGTGTTCT AAAGGTGCCT CCGGAGGAAC GATTGATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937280.2
    CDS191..4561
    Translation

    Target ORF information:

    RefSeq Version XM_002937234.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis crumbs 2, cell polarity complex component (crb2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937234.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTACA GAAGCGTTAG TCGCACTTCA CCCAGTCCCG TTATTCTGCT ACTGCTACTT 
    CTCTTCTCCT GCGGGCAAGG ACTTGCCCAG ACCTGTGTGT CGTCCCCTTG CCTGAATGGT
    GGAACATGCC AGGAAACAGC AAGTGGATTT ACCTGTCTGT GCCCAGCGGA GCCATTGGCA
    TTTACTGGAA CTACCTGTGG AGATCTGTAT GATGCCTGTG CCATTGATAG ATGCCCCAGC
    ACCCATAGCT GTCAGAGCAC CCCAGGGCAT CCTCATTTTC AGTGTGTTTG CCAACCTGGG
    TTGAACTGCA CCAATGAGTG TCAGAGAAAT TCCTGCCCAT TCCAAAATTT TCAGTGTACA
    GTTGGTGAGG ACAGCTTTAT GTGCAAGTGC CAAAGTGGGT ACGGTGGAGT GGACTGCAAC
    TTTGAGGTAT CACCGTGTTT TCAGAACCCC TGCCAGAACA ATGCCACCTG TTTGGCAGAA
    GGAGACAGCT TCAGGTGTGT ATGTGAGCCA GGGTTCACAG GGCAGGAATG TGAGGTCAAT
    GTGAACGAAT GTGCTTCAAA CCCATGCCAG AACGAGGCTC TCTGTGTGGA TAAAGTGGAC
    AGATACATGT GCTTCTGTGT GCCCGGCTTC CAAGGCCACC ATTGCGAAAT AGACATCAAC
    GAGTGTGCTT CCAGACCATG TCAGAACAAC GGAACTTGTA TGAACCAACT GGACCAATAT
    GAATGTGCTT GTGCCAATGG TTACACAGGA ATTAACTGTG AGATGGAGAT AGATGAATGT
    CAGTCAGTTC CATGTCAGAA TGGAGCTACA TGTAGGGACC ATATTGGCTA CTTCACGTGT
    GACTGCCCAG CGGGCTATGA TGGAGAACAG TGCCAGCATG ACATTGATGA GTGCCAGAGC
    CAGCCCTGTC AGAATGGAGG GCAGTGTATG GATGAAATTA ACAGGTTCCG CTGTGATTGC
    AGTAATACTG GCTTTGTTGG TGGCCGCTGT GAAGTAGAAA TCCTGGAGTG TGACTCAAAT
    CCTTGTCAGA ACAATGGCAC GTGCATGGAG AGAGTCAATG GCTACGAATG CTTGTGCTGG
    AAAGGTTATT CTGGAATTCA TTGTGAAATA GATGATAATG AATGTTTTGA GGAGCCATGT
    GTAAATGGTG GCCTATGTCT TGAACTATCC AACCAGTCAT ATTATGGCAC TGAACCAGAA
    TTTAACAAGT TCAGCTACAG CCAAGCAGCA GGATATTTGT GCCGCTGCCA GCAAGGATTT
    ACAGGACCAA ACTGCTCTGT GAACATTGAT GAATGTGAAT CTTGGCCTTG TCTGAATGGC
    GGAACCTGCA CCGACTTAAT CAATGGCTTC CTGTGTAACT GTGCTTCTGG ATATACAGGT
    TTCGACTGTT CCATAAACAT CGATGAGTGT AAAGACAGCC CATGTAAGAA TGGGGCAAGC
    TGTGAAGATG GAGTTGCTGA CTACTCCTGC ATCTGTCCAC CAGCTGCCCA GGATGGGACA
    GTATGGGGTG GTAAAAACTG TTCTGTAATG TTGATTGGAT GCTTGGAGCA CAGCTGCCAA
    AACGCAGCAG AGTGCATCCC TTTATATACT GGACAAAGCC ACAGTTATAC CTGCAAATGT
    CAACCAGGTT TTGTTGGGAA CAATTGTTCA ACCCCTACAA CATTTTCCTT CTTGTCCCAG
    GGGTACCTAA TGTATGATTC ACCTTTAAGT AATGAATCGT ATCAAAGCAA CATGTCAGTG
    AGATTCAGGA CAACATTACC TGACATGGTC CTCATGTACC GAGGAGATGA AGATAGCTTT
    CTCGCACTTG AGATTTATAA TGGATTTCTG CACATGCGCC TTAAGGGAAA TGGAAGGTTG
    TTTCCGCTGG TTATTGATGA TTACAAGGTG AACGATGGCC ACTGGCACAA TGTGGAGGTA
    GACCTTCTAT CAAATTCATT CTTGAAGATC AGCCTTTTTC ATAATGATTG CCGTAATGGA
    CTCTGCTCCC AAAAACAGAC ACCCACTGAA GGATTTGACC CCTATCTTCC AGAGTCCTTC
    AGATCATTGT ATATTGGAGG ACTTATTCAA GAGTCATTAA TCAAGAACAC TCTAAGTCAG
    CAAAACTTCA CTGGCTGCCT GGAGGACTTG GTTGTTGACT TGGTTACTCA GCTGCCGCAC
    AACGTAAGCA GTGACAGGTC CTTTGGAATG GTACTCGGAT GCAAAAAGGC GGAGGTGTGC
    CATCCAAACC CATGTCACAA TGGGGCAAAC TGCATTGATC TCTGGTCCAG CTTTACATGT
    GAATGCATCA GGCCCTTTAA AGGGTCCACC TGTATGCAGG AGTATATAGC AGGAACATTC
    TTCAGGGAGG GGATTCCAAG CCACTCTTAC TTTGAACTCA ATCAGGATTT CGGAACGTCT
    TTCAATATAT CTGCTTTTAT CAGAACCCTA AAATCGAATG GTCTCCTACT GCAAATCAGC
    TACGGAACAA CGCCTTACTT CAGAACTTAC CTGAATAATA GTTGGATCCA CATTGAGACA
    CTATCTGCAC AACCTCTTGT CTTTAAGAAG AACGTCAGTG ATGGCATGAA GCACTTTATA
    AGTATTTCTG TCAGGGAAGG TAACGTTACA GTCACTGATC TTCAGCAGGA AGTTTACTTG
    GGAATGTTGC CGCCGGTGAT CATTACCAAA GGAGATGCAG TCCATGTGGG CGGTTTGGCT
    ACAGCAGACA GTACTATTTT TTGGGGTGGG TATTTCAAAG GATGTGTTCA AGATTTAAGG
    ATTAACAACG ATCACTTGGA GTTCTTCCAC CCAGAACATA TAAATGTACA AGAAACCTTA
    TACTTTGGGA ACAACACCAA CGTCACTGAG AACTGCATCT CTGATGATGT TTGTCATCAA
    GGCCCATGCA GAAATGAAGG AAACTGCTCA GTAACCTGGA ATGACTTTGT TTGCAGCTGT
    CCGGCCAACT TCACCGGAAA AAGATGTGAG GAGCCGGTTT GGTGTCGACG CTCTCCCTGC
    TTGCCAGGCT CCACTTGCGT GGATGTTCCC AAAGGATTTG TGTGTCTGGC CAATGTGAGC
    TTCCAGGGCC ACAGCTCTGC AGTATTCACT CCCATTATTT CAGAAGAACA CAATCTTACA
    TCTATCTCAT TGGCCTTCAG AACCAGAGAC AGAGATGCAG TTTTGCTCCA GTCTTCCAAA
    AATGTGGATT CTGTGCTAAT AGAAATTCAG GGGGGTTACT TGTTTGTTGA TCTGCAAAGT
    GGAAATAATG TGGATGGAGT CCAGCTTAAT GGAACAATAG ATGTTGCTGA TGCTATCTGG
    CACAGAGTAG TAATAACCAT GGAAAGAGCG ACTAAAGTGA ATTCTCATTG GACATTCCAG
    CTGGATGACA GTGTTCAAAA GAAATTTCAG TGGGAAGGCG ACAACTTGAA TTTTATGAGA
    AATAACATTT CCATTGTATT AGCTGAAGGC TACACTGGAT GTTTGGGCGA TGTGTCAATT
    GGAGGGATTT ACCTTCCATT TACAGACCAT GTGTTGCCAC AGCAGGATCA GTTTATAAGG
    AAATCTGATG CTTTGGAGTT GGGATGCAGA GGGGCAGATG TTTGTTCTGA GAGTCCCTGT
    CTTCATGGTG GAACATGCCA AGATCTTTTT AACTCCTTTA GCTGTACGTG CAGAATAGGG
    TGGAATGGGC ATCGTTGTGA GATCAACATC GATGACTGCA AGTCTAGCCC ATGTCTTCAT
    GGTTTTTGTA TAGACCTAGA GGCTGATTAT CAGTGCAGCT GTGAGGCTGG TTATACCAGC
    AGGAACTGCG AAATCAACAT CAATGACTGT CAGCACCATC AGTGCCTGAA TGGGGGGACT
    TGTGTTGATG GCGTAAATAA CTACACATGC AAGTGCCCAG CCAACTATGC GGGGACCTTC
    TGCCAATGGC CTTTTCCACC TGAGCAGTGC AATAAAAACA TCACGTGTTT TAATGGAGGC
    AGATGCAACA GTGGAATTTG GGGAGCTAAC TGCACATGTA GACCAGGCTT TAGTGGCAGA
    AGGTGTGAAA TAAATGTCAA CGATTGTGAA TCAAACCCAT GTCTCAATGG TGGAACTTGC
    CAGGATTCTG TAAATACCTT CAAATGCATT TGCAATTCCA GCTTTAGTGG GGTGCGCTGT
    GAGAAAAGCG ATGCAGCCTC AATGTTCCCC TTTCCTCTTG TTGGAGTTGC AGTACCTGTA
    ACCTGTGGGG TTTTACTTCT ACTTGTTATT GGGGTCATAT TCATGGTGCT GACAGCAAGG
    AAGAGGAGAC AGTCAGAAGG AACCTACAGC CCAAGCCAAC AAGAAGTTGC AGGAGCACGA
    CTTGAAATGG ACAGTGTTCT AAAGGTGCCT CCGGAGGAAC GATTGATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa17942
    Clone ID Related Accession (Same CDS sequence) XM_004918924.3 , XM_004918924.4
    Accession Version XM_004918924.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein crumbs homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004918924.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTACA GAAGCGTTAG TCGCACTTCA CCCAGTCCCG TTATTCTGCT ACTGCTACTT 
    CTCTTCTCCT GCGGGCAAGG ACTTGCCCAG ACCTGTGTGT CGTCCCCTTG CCTGAATGGT
    GGAACATGCC AGGAAACAGC AAGTGGATTT ACCTGTCTGT GCCCAGCGGA GCCATTGGCA
    TTTACTGGAA CTACCTGTGG AGATCTGTAT GATGCCTGTG CCATTGATAG ATGCCCCAGC
    ACCCATAGCT GTCAGAGCAC CCCAGGGCAT CCTCATTTTC AGTGTGTTTG CCAACCTGGG
    TTGAACTGCA CCAATGAGTG TCAGAGAAAT TCCTGCCCAT TCCAAAATTT TCAGTGTACA
    GTTGGTGAGG ACAGCTTTAT GTGCAAGTGC CAAAGTGGGT ACGGTGGAGT GGACTGCAAC
    TTTGAGGTAT CACCGTGTTT TCAGAACCCC TGCCAGAACA ATGCCACCTG TTTGGCAGAA
    GGAGACAGCT TCAGGTGTGT ATGTGAGCCA GGGTTCACAG GGCAGGAATG TGAGGTCAAT
    GTGAACGAAT GTGCTTCAAA CCCATGCCAG AACGAGGCTC TCTGTGTGGA TAAAGTGGAC
    AGATACATGT GCTTCTGTGT GCCCGGCTTC CAAGGCCACC ATTGCGAAAT AGACATCAAC
    GAGTGTGCTT CCAGACCATG TCAGAACAAC GGAACTTGTA TGAACCAACT GGACCAATAT
    GAATGTGCTT GTGCCAATGG TTACACAGGA ATTAACTGTG AGATGGAGAT AGATGAATGT
    CAGTCAGTTC CATGTCAGAA TGGAGCTACA TGTAGGGACC ATATTGGCTA CTTCACGTGT
    GACTGCCCAG CGGGCTATGA TGGAGAACAG TGCCAGCATG ACATTGATGA GTGCCAGAGC
    CAGCCCTGTC AGAATGGAGG GCAGTGTATG GATGAAATTA ACAGGTTCCG CTGTGATTGC
    AGTAATACTG GCTTTGTTGG TGGCCGCTGT GAAGTAGAAA TCCTGGAGTG TGACTCAAAT
    CCTTGTCAGA ACAATGGCAC GTGCATGGAG AGAGTCAATG GCTACGAATG CTTGTGCTGG
    AAAGGTTATT CTGGAATTCA TTGTGAAATA GATGATAATG AATGTTTTGA GGAGCCATGT
    GTAAATGGTG GCCTATGTCT TGAACTATCC AACCAGTCAT ATTATGGCAC TGAACCAGAA
    TTTAACAAGT TCAGCTACAG CCAAGCAGCA GGATATTTGT GCCGCTGCCA GCAAGGATTT
    ACAGGACCAA ACTGCTCTGT GAACATTGAT GAATGTGAAT CTTGGCCTTG TCTGAATGGC
    GGAACCTGCA CCGACTTAAT CAATGGCTTC CTGTGTAACT GTGCTTCTGG ATATACAGGT
    TTCGACTGTT CCATAAACAT CGATGAGTGT AAAGACAGCC CATGTAAGAA TGGGGCAAGC
    TGTGAAGATG GAGTTGCTGA CTACTCCTGC ATCTGTCCAC CAGCTGCCCA GGATGGGACA
    GTATGGGGTG GTAAAAACTG TTCTGTAATG TTGATTGGAT GCTTGGAGCA CAGCTGCCAA
    AACGCAGCAG AGTGCATCCC TTTATATACT GGACAAAGCC ACAGTTATAC CTGCAAATGT
    CAACCAGGTT TTGTTGGGAA CAATTGTTCA ACCCCTACAA CATTTTCCTT CTTGTCCCAG
    GGGTACCTAA TGTATGATTC ACCTTTAAGT AATGAATCGT ATCAAAGCAA CATGTCAGTG
    AGATTCAGGA CAACATTACC TGACATGGTC CTCATGTACC GAGGAGATGA AGATAGCTTT
    CTCGCACTTG AGATTTATAA TGGATTTCTG CACATGCGCC TTAAGGGAAA TGGAAGGTTG
    TTTCCGCTGG TTATTGATGA TTACAAGGTG AACGATGGCC ACTGGCACAA TGTGGAGGTA
    GACCTTCTAT CAAATTCATT CTTGAAGATC AGCCTTTTTC ATAATGATTG CCGTAATGGA
    CTCTGCTCCC AAAAACAGAC ACCCACTGAA GGATTTGACC CCTATCTTCC AGAGTCCTTC
    AGATCATTGT ATATTGGAGG ACTTATTCAA GAGTCATTAA TCAAGAACAC TCTAAGTCAG
    CAAAACTTCA CTGGCTGCCT GGAGGACTTG GTTGTTGACT TGGTTACTCA GCTGCCGCAC
    AACGTAAGCA GTGACAGGTC CTTTGGAATG GTACTCGGAT GCAAAAAGGC GGAGGTGTGC
    CATCCAAACC CATGTCACAA TGGGGCAAAC TGCATTGATC TCTGGTCCAG CTTTACATGT
    GAATGCATCA GGCCCTTTAA AGGGTCCACC TGTATGCAGG AGTATATAGC AGGAACATTC
    TTCAGGGAGG GGATTCCAAG CCACTCTTAC TTTGAACTCA ATCAGGATTT CGGAACGTCT
    TTCAATATAT CTGCTTTTAT CAGAACCCTA AAATCGAATG GTCTCCTACT GCAAATCAGC
    TACGGAACAA CGCCTTACTT CAGAACTTAC CTGAATAATA GTTGGATCCA CATTGAGACA
    CTATCTGCAC AACCTCTTGT CTTTAAGAAG AACGTCAGTG ATGGCATGAA GCACTTTATA
    AGTATTTCTG TCAGGGAAGG TAACGTTACA GTCACTGATC TTCAGCAGGA AGTTTACTTG
    GGAATGTTGC CGCCGGTGAT CATTACCAAA GGAGATGCAG TCCATGTGGG CGGTTTGGCT
    ACAGCAGACA GTACTATTTT TTGGGGTGGG TATTTCAAAG GATGTGTTCA AGATTTAAGG
    ATTAACAACG ATCACTTGGA GTTCTTCCAC CCAGAACATA TAAATGTACA AGAAACCTTA
    TACTTTGGGA ACAACACCAA CGTCACTGAG AACTGCATCT CTGATGATGT TTGTCATCAA
    GGCCCATGCA GAAATGAAGG AAACTGCTCA GTAACCTGGA ATGACTTTGT TTGCAGCTGT
    CCGGCCAACT TCACCGGAAA AAGATGTGAG GAGCCGGTTT GGTGTCGACG CTCTCCCTGC
    TTGCCAGGCT CCACTTGCGT GGATGTTCCC AAAGGATTTG TGTGTCTGGC CAATGTGAGC
    TTCCAGGGCC ACAGCTCTGC AGTATTCACT CCCATTATTT CAGAAGAACA CAATCTTACA
    TCTATCTCAT TGGCCTTCAG AACCAGAGAC AGAGATGCAG TTTTGCTCCA GTCTTCCAAA
    AATGTGGATT CTGTGCTAAT AGAAATTCAG GGGGGTTACT TGTTTGTTGA TCTGCAAAGT
    GGAAATAATG TGGATGGAGT CCAGCTTAAT GGAACAATAG ATGTTGCTGA TGCTATCTGG
    CACAGAGTAG TAATAACCAT GGAAAGAGCG ACTAAAGTGA ATTCTCATTG GACATTCCAG
    CTGGATGACA GTGTTCAAAA GAAATTTCAG TGGGAAGGCG ACAACTTGAA TTTTATGAGA
    AATAACATTT CCATTGTATT AGCTGAAGGC TACACTGGAT GTTTGGGCGA TGTGTCAATT
    GGAGGGATTT ACCTTCCATT TACAGACCAT GTGTTGCCAC AGCAGGATCA GTTTATAAGG
    AAATCTGATG CTTTGGAGTT GGGATGCAGA GGGGCAGATG TTTGTTCTGA GAGTCCCTGT
    CTTCATGGTG GAACATGCCA AGATCTTTTT AACTCCTTTA GCTGTACGTG CAGAATAGGG
    TGGAATGGGC ATCGTTGTGA GATCAACATC GATGACTGCA AGTCTAGCCC ATGTCTTCAT
    GGTTTTTGTA TAGACCTAGA GGCTGATTAT CAGTGCAGCT GTGAGGCTGG TTATACCAGC
    AGGAACTGCG AAATCAACAT CAATGACTGT CAGCACCATC AGTGCCTGAA TGGGGGGACT
    TGTGTTGATG GCGTAAATAA CTACACATGC AAGTGCCCAG CCAACTATGC GGGGACCTTC
    TGCCAATGGC CTTTTCCACC TGAGCAGTGC AATAAAAACA TCACGTGTTT TAATGGAGGC
    AGATGCAACA GTGGAATTTG GGGAGCTAAC TGCACATGTA GACCAGGCTT TAGTGGCAGA
    AGGTGTGAAA TAAATGTCAA CGATTGTGAA TCAAACCCAT GTCTCAATGG TGGAACTTGC
    CAGGATTCTG TAAATACCTT CAAATGCATT TGCAATTCCA GCTTTAGTGG GGTGCGCTGT
    GAGAAGCCAC GCCTGCACCG TGAAGCAAAG GCCTCCACTC TGGTTGGGTC GGCAATAGGA
    ACAGGAATGC TCTTCATACT GCTTTCTGTC ATCACTGCAG TTTTGGTGAC TATGAGAAAT
    AAGAGAGCAA CTCAGGGGAC TTACAGTCCC AGCCGCCAGG AGAAGGATGG GGCTCGAGTG
    GAAATGTGGA ATGTCCTGAA GCTTCCACCC ACCGAGCGTC TTATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004918981.1
    CDS193..4560
    Translation

    Target ORF information:

    RefSeq Version XM_004918924.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis crumbs 2, cell polarity complex component (crb2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004918924.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTACA GAAGCGTTAG TCGCACTTCA CCCAGTCCCG TTATTCTGCT ACTGCTACTT 
    CTCTTCTCCT GCGGGCAAGG ACTTGCCCAG ACCTGTGTGT CGTCCCCTTG CCTGAATGGT
    GGAACATGCC AGGAAACAGC AAGTGGATTT ACCTGTCTGT GCCCAGCGGA GCCATTGGCA
    TTTACTGGAA CTACCTGTGG AGATCTGTAT GATGCCTGTG CCATTGATAG ATGCCCCAGC
    ACCCATAGCT GTCAGAGCAC CCCAGGGCAT CCTCATTTTC AGTGTGTTTG CCAACCTGGG
    TTGAACTGCA CCAATGAGTG TCAGAGAAAT TCCTGCCCAT TCCAAAATTT TCAGTGTACA
    GTTGGTGAGG ACAGCTTTAT GTGCAAGTGC CAAAGTGGGT ACGGTGGAGT GGACTGCAAC
    TTTGAGGTAT CACCGTGTTT TCAGAACCCC TGCCAGAACA ATGCCACCTG TTTGGCAGAA
    GGAGACAGCT TCAGGTGTGT ATGTGAGCCA GGGTTCACAG GGCAGGAATG TGAGGTCAAT
    GTGAACGAAT GTGCTTCAAA CCCATGCCAG AACGAGGCTC TCTGTGTGGA TAAAGTGGAC
    AGATACATGT GCTTCTGTGT GCCCGGCTTC CAAGGCCACC ATTGCGAAAT AGACATCAAC
    GAGTGTGCTT CCAGACCATG TCAGAACAAC GGAACTTGTA TGAACCAACT GGACCAATAT
    GAATGTGCTT GTGCCAATGG TTACACAGGA ATTAACTGTG AGATGGAGAT AGATGAATGT
    CAGTCAGTTC CATGTCAGAA TGGAGCTACA TGTAGGGACC ATATTGGCTA CTTCACGTGT
    GACTGCCCAG CGGGCTATGA TGGAGAACAG TGCCAGCATG ACATTGATGA GTGCCAGAGC
    CAGCCCTGTC AGAATGGAGG GCAGTGTATG GATGAAATTA ACAGGTTCCG CTGTGATTGC
    AGTAATACTG GCTTTGTTGG TGGCCGCTGT GAAGTAGAAA TCCTGGAGTG TGACTCAAAT
    CCTTGTCAGA ACAATGGCAC GTGCATGGAG AGAGTCAATG GCTACGAATG CTTGTGCTGG
    AAAGGTTATT CTGGAATTCA TTGTGAAATA GATGATAATG AATGTTTTGA GGAGCCATGT
    GTAAATGGTG GCCTATGTCT TGAACTATCC AACCAGTCAT ATTATGGCAC TGAACCAGAA
    TTTAACAAGT TCAGCTACAG CCAAGCAGCA GGATATTTGT GCCGCTGCCA GCAAGGATTT
    ACAGGACCAA ACTGCTCTGT GAACATTGAT GAATGTGAAT CTTGGCCTTG TCTGAATGGC
    GGAACCTGCA CCGACTTAAT CAATGGCTTC CTGTGTAACT GTGCTTCTGG ATATACAGGT
    TTCGACTGTT CCATAAACAT CGATGAGTGT AAAGACAGCC CATGTAAGAA TGGGGCAAGC
    TGTGAAGATG GAGTTGCTGA CTACTCCTGC ATCTGTCCAC CAGCTGCCCA GGATGGGACA
    GTATGGGGTG GTAAAAACTG TTCTGTAATG TTGATTGGAT GCTTGGAGCA CAGCTGCCAA
    AACGCAGCAG AGTGCATCCC TTTATATACT GGACAAAGCC ACAGTTATAC CTGCAAATGT
    CAACCAGGTT TTGTTGGGAA CAATTGTTCA ACCCCTACAA CATTTTCCTT CTTGTCCCAG
    GGGTACCTAA TGTATGATTC ACCTTTAAGT AATGAATCGT ATCAAAGCAA CATGTCAGTG
    AGATTCAGGA CAACATTACC TGACATGGTC CTCATGTACC GAGGAGATGA AGATAGCTTT
    CTCGCACTTG AGATTTATAA TGGATTTCTG CACATGCGCC TTAAGGGAAA TGGAAGGTTG
    TTTCCGCTGG TTATTGATGA TTACAAGGTG AACGATGGCC ACTGGCACAA TGTGGAGGTA
    GACCTTCTAT CAAATTCATT CTTGAAGATC AGCCTTTTTC ATAATGATTG CCGTAATGGA
    CTCTGCTCCC AAAAACAGAC ACCCACTGAA GGATTTGACC CCTATCTTCC AGAGTCCTTC
    AGATCATTGT ATATTGGAGG ACTTATTCAA GAGTCATTAA TCAAGAACAC TCTAAGTCAG
    CAAAACTTCA CTGGCTGCCT GGAGGACTTG GTTGTTGACT TGGTTACTCA GCTGCCGCAC
    AACGTAAGCA GTGACAGGTC CTTTGGAATG GTACTCGGAT GCAAAAAGGC GGAGGTGTGC
    CATCCAAACC CATGTCACAA TGGGGCAAAC TGCATTGATC TCTGGTCCAG CTTTACATGT
    GAATGCATCA GGCCCTTTAA AGGGTCCACC TGTATGCAGG AGTATATAGC AGGAACATTC
    TTCAGGGAGG GGATTCCAAG CCACTCTTAC TTTGAACTCA ATCAGGATTT CGGAACGTCT
    TTCAATATAT CTGCTTTTAT CAGAACCCTA AAATCGAATG GTCTCCTACT GCAAATCAGC
    TACGGAACAA CGCCTTACTT CAGAACTTAC CTGAATAATA GTTGGATCCA CATTGAGACA
    CTATCTGCAC AACCTCTTGT CTTTAAGAAG AACGTCAGTG ATGGCATGAA GCACTTTATA
    AGTATTTCTG TCAGGGAAGG TAACGTTACA GTCACTGATC TTCAGCAGGA AGTTTACTTG
    GGAATGTTGC CGCCGGTGAT CATTACCAAA GGAGATGCAG TCCATGTGGG CGGTTTGGCT
    ACAGCAGACA GTACTATTTT TTGGGGTGGG TATTTCAAAG GATGTGTTCA AGATTTAAGG
    ATTAACAACG ATCACTTGGA GTTCTTCCAC CCAGAACATA TAAATGTACA AGAAACCTTA
    TACTTTGGGA ACAACACCAA CGTCACTGAG AACTGCATCT CTGATGATGT TTGTCATCAA
    GGCCCATGCA GAAATGAAGG AAACTGCTCA GTAACCTGGA ATGACTTTGT TTGCAGCTGT
    CCGGCCAACT TCACCGGAAA AAGATGTGAG GAGCCGGTTT GGTGTCGACG CTCTCCCTGC
    TTGCCAGGCT CCACTTGCGT GGATGTTCCC AAAGGATTTG TGTGTCTGGC CAATGTGAGC
    TTCCAGGGCC ACAGCTCTGC AGTATTCACT CCCATTATTT CAGAAGAACA CAATCTTACA
    TCTATCTCAT TGGCCTTCAG AACCAGAGAC AGAGATGCAG TTTTGCTCCA GTCTTCCAAA
    AATGTGGATT CTGTGCTAAT AGAAATTCAG GGGGGTTACT TGTTTGTTGA TCTGCAAAGT
    GGAAATAATG TGGATGGAGT CCAGCTTAAT GGAACAATAG ATGTTGCTGA TGCTATCTGG
    CACAGAGTAG TAATAACCAT GGAAAGAGCG ACTAAAGTGA ATTCTCATTG GACATTCCAG
    CTGGATGACA GTGTTCAAAA GAAATTTCAG TGGGAAGGCG ACAACTTGAA TTTTATGAGA
    AATAACATTT CCATTGTATT AGCTGAAGGC TACACTGGAT GTTTGGGCGA TGTGTCAATT
    GGAGGGATTT ACCTTCCATT TACAGACCAT GTGTTGCCAC AGCAGGATCA GTTTATAAGG
    AAATCTGATG CTTTGGAGTT GGGATGCAGA GGGGCAGATG TTTGTTCTGA GAGTCCCTGT
    CTTCATGGTG GAACATGCCA AGATCTTTTT AACTCCTTTA GCTGTACGTG CAGAATAGGG
    TGGAATGGGC ATCGTTGTGA GATCAACATC GATGACTGCA AGTCTAGCCC ATGTCTTCAT
    GGTTTTTGTA TAGACCTAGA GGCTGATTAT CAGTGCAGCT GTGAGGCTGG TTATACCAGC
    AGGAACTGCG AAATCAACAT CAATGACTGT CAGCACCATC AGTGCCTGAA TGGGGGGACT
    TGTGTTGATG GCGTAAATAA CTACACATGC AAGTGCCCAG CCAACTATGC GGGGACCTTC
    TGCCAATGGC CTTTTCCACC TGAGCAGTGC AATAAAAACA TCACGTGTTT TAATGGAGGC
    AGATGCAACA GTGGAATTTG GGGAGCTAAC TGCACATGTA GACCAGGCTT TAGTGGCAGA
    AGGTGTGAAA TAAATGTCAA CGATTGTGAA TCAAACCCAT GTCTCAATGG TGGAACTTGC
    CAGGATTCTG TAAATACCTT CAAATGCATT TGCAATTCCA GCTTTAGTGG GGTGCGCTGT
    GAGAAGCCAC GCCTGCACCG TGAAGCAAAG GCCTCCACTC TGGTTGGGTC GGCAATAGGA
    ACAGGAATGC TCTTCATACT GCTTTCTGTC ATCACTGCAG TTTTGGTGAC TATGAGAAAT
    AAGAGAGCAA CTCAGGGGAC TTACAGTCCC AGCCGCCAGG AGAAGGATGG GGCTCGAGTG
    GAAATGTGGA ATGTCCTGAA GCTTCCACCC ACCGAGCGTC TTATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa64134
    Clone ID Related Accession (Same CDS sequence) XM_031890901.1
    Accession Version XM_031890901.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein crumbs homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCAAGGAC TTGCCCAGAC CTGTGTGTCG TCCCCTTGCC TGAATGGTGG AACATGCCAG 
    GAAACAGCAA GTGGATTTAC CTGTCTGTGC CCAGCGGAGC CATTGGCATT TACTGGAACT
    ACCTGTGGAG ATCTGTATGA TGCCTGTGCC ATTGATAGAT GCCCCAGCAC CCATAGCTGT
    CAGAGCACCC CAGGGCATCC TCATTTTCAG TGTGTTTGCC AACCTGGGTT GAACTGCACC
    AATGAGTGTC AGAGAAATTC CTGCCCATTC CAAAATTTTC AGTGTACAGT TGGTGAGGAC
    AGCTTTATGT GCAAGTGCCA AAGTGGGTAC GGTGGAGTGG ACTGCAACTT TGAGGTATCA
    CCGTGTTTTC AGAACCCCTG CCAGAACAAT GCCACCTGTT TGGCAGAAGG AGACAGCTTC
    AGGTGTGTAT GTGAGCCAGG GTTCACAGGG CAGGAATGTG AGGTCAATGT GAACGAATGT
    GCTTCAAACC CATGCCAGAA CGAGGCTCTC TGTGTGGATA AAGTGGACAG ATACATGTGC
    TTCTGTGTGC CCGGCTTCCA AGGCCACCAT TGCGAAATAG ACATCAACGA GTGTGCTTCC
    AGACCATGTC AGAACAACGG AACTTGTATG AACCAACTGG ACCAATATGA ATGTGCTTGT
    GCCAATGGTT ACACAGGAAT TAACTGTGAG ATGGAGATAG ATGAATGTCA GTCAGTTCCA
    TGTCAGAATG GAGCTACATG TAGGGACCAT ATTGGCTACT TCACGTGTGA CTGCCCAGCG
    GGCTATGATG GAGAACAGTG CCAGCATGAC ATTGATGAGT GCCAGAGCCA GCCCTGTCAG
    AATGGAGGGC AGTGTATGGA TGAAATTAAC AGGTTCCGCT GTGATTGCAG TAATACTGGC
    TTTGTTGGTG GCCGCTGTGA AGTAGAAATC CTGGAGTGTG ACTCAAATCC TTGTCAGAAC
    AATGGCACGT GCATGGAGAG AGTCAATGGC TACGAATGCT TGTGCTGGAA AGGTTATTCT
    GGAATTCATT GTGAAATAGA TGATAATGAA TGTTTTGAGG AGCCATGTGT AAATGGTGGC
    CTATGTCTTG AACTATCCAA CCAGTCATAT TATGGCACTG AACCAGAATT TAACAAGTTC
    AGCTACAGCC AAGCAGCAGG ATATTTGTGC CGCTGCCAGC AAGGATTTAC AGGACCAAAC
    TGCTCTGTGA ACATTGATGA ATGTGAATCT TGGCCTTGTC TGAATGGCGG AACCTGCACC
    GACTTAATCA ATGGCTTCCT GTGTAACTGT GCTTCTGGAT ATACAGGTTT CGACTGTTCC
    ATAAACATCG ATGAGTGTAA AGACAGCCCA TGTAAGAATG GGGCAAGCTG TGAAGATGGA
    GTTGCTGACT ACTCCTGCAT CTGTCCACCA GCTGCCCAGG ATGGGACAGT ATGGGGTGGT
    AAAAACTGTT CTGTAATGTT GATTGGATGC TTGGAGCACA GCTGCCAAAA CGCAGCAGAG
    TGCATCCCTT TATATACTGG ACAAAGCCAC AGTTATACCT GCAAATGTCA ACCAGGTTTT
    GTTGGGAACA ATTGTTCAAC CCCTACAACA TTTTCCTTCT TGTCCCAGGG GTACCTAATG
    TATGATTCAC CTTTAAGTAA TGAATCGTAT CAAAGCAACA TGTCAGTGAG ATTCAGGACA
    ACATTACCTG ACATGGTCCT CATGTACCGA GGAGATGAAG ATAGCTTTCT CGCACTTGAG
    ATTTATAATG GATTTCTGCA CATGCGCCTT AAGGGAAATG GAAGGTTGTT TCCGCTGGTT
    ATTGATGATT ACAAGGTGAA CGATGGCCAC TGGCACAATG TGGAGGTAGA CCTTCTATCA
    AATTCATTCT TGAAGATCAG CCTTTTTCAT AATGATTGCC GTAATGGACT CTGCTCCCAA
    AAACAGACAC CCACTGAAGG ATTTGACCCC TATCTTCCAG AGTCCTTCAG ATCATTGTAT
    ATTGGAGGAC TTATTCAAGA GTCATTAATC AAGAACACTC TAAGTCAGCA AAACTTCACT
    GGCTGCCTGG AGGACTTGGT TGTTGACTTG GTTACTCAGC TGCCGCACAA CGTAAGCAGT
    GACAGGTCCT TTGGAATGGT ACTCGGATGC AAAAAGGCGG AGGTGTGCCA TCCAAACCCA
    TGTCACAATG GGGCAAACTG CATTGATCTC TGGTCCAGCT TTACATGTGA ATGCATCAGG
    CCCTTTAAAG GGTCCACCTG TATGCAGGAG TATATAGCAG GAACATTCTT CAGGGAGGGG
    ATTCCAAGCC ACTCTTACTT TGAACTCAAT CAGGATTTCG GAACGTCTTT CAATATATCT
    GCTTTTATCA GAACCCTAAA ATCGAATGGT CTCCTACTGC AAATCAGCTA CGGAACAACG
    CCTTACTTCA GAACTTACCT GAATAATAGT TGGATCCACA TTGAGACACT ATCTGCACAA
    CCTCTTGTCT TTAAGAAGAA CGTCAGTGAT GGCATGAAGC ACTTTATAAG TATTTCTGTC
    AGGGAAGGTA ACGTTACAGT CACTGATCTT CAGCAGGAAG TTTACTTGGG AATGTTGCCG
    CCGGTGATCA TTACCAAAGG AGATGCAGTC CATGTGGGCG GTTTGGCTAC AGCAGACAGT
    ACTATTTTTT GGGGTGGGTA TTTCAAAGGA TGTGTTCAAG ATTTAAGGAT TAACAACGAT
    CACTTGGAGT TCTTCCACCC AGAACATATA AATGTACAAG AAACCTTATA CTTTGGGAAC
    AACACCAACG TCACTGAGAA CTGCATCTCT GATGATGTTT GTCATCAAGG CCCATGCAGA
    AATGAAGGAA ACTGCTCAGT AACCTGGAAT GACTTTGTTT GCAGCTGTCC GGCCAACTTC
    ACCGGAAAAA GATGTGAGGA GCCGGTTTGG TGTCGACGCT CTCCCTGCTT GCCAGGCTCC
    ACTTGCGTGG ATGTTCCCAA AGGATTTGTG TGTCTGGCCA ATGTGAGCTT CCAGGGCCAC
    AGCTCTGCAG TATTCACTCC CATTATTTCA GAAGAACACA ATCTTACATC TATCTCATTG
    GCCTTCAGAA CCAGAGACAG AGATGCAGTT TTGCTCCAGT CTTCCAAAAA TGTGGATTCT
    GTGCTAATAG AAATTCAGGG GGGTTACTTG TTTGTTGATC TGCAAAGTGG AAATAATGTG
    GATGGAGTCC AGCTTAATGG AACAATAGAT GTTGCTGATG CTATCTGGCA CAGAGTAGTA
    ATAACCATGG AAAGAGCGAC TAAAGTGAAT TCTCATTGGA CATTCCAGCT GGATGACAGT
    GTTCAAAAGA AATTTCAGTG GGAAGGCGAC AACTTGAATT TTATGAGAAA TAACATTTCC
    ATTGTATTAG CTGAAGGCTA CACTGGATGT TTGGGCGATG TGTCAATTGG AGGGATTTAC
    CTTCCATTTA CAGACCATGT GTTGCCACAG CAGGATCAGT TTATAAGGAA ATCTGATGCT
    TTGGAGTTGG GATGCAGAGG GGCAGATGTT TGTTCTGAGA GTCCCTGTCT TCATGGTGGA
    ACATGCCAAG ATCTTTTTAA CTCCTTTAGC TGTACGTGCA GAATAGGGTG GAATGGGCAT
    CGTTGTGAGA TCAACATCGA TGACTGCAAG TCTAGCCCAT GTCTTCATGG TTTTTGTATA
    GACCTAGAGG CTGATTATCA GTGCAGCTGT GAGGCTGGTT ATACCAGCAG GAACTGCGAA
    ATCAACATCA ATGACTGTCA GCACCATCAG TGCCTGAATG GGGGGACTTG TGTTGATGGC
    GTAAATAACT ACACATGCAA GTGCCCAGCC AACTATGCGG GGACCTTCTG CCAATGGCCT
    TTTCCACCTG AGCAGTGCAA TAAAAACATC ACGTGTTTTA ATGGAGGCAG ATGCAACAGT
    GGAATTTGGG GAGCTAACTG CACATGTAGA CCAGGCTTTA GTGGCAGAAG GTGTGAAATA
    AATGTCAACG ATTGTGAATC AAACCCATGT CTCAATGGTG GAACTTGCCA GGATTCTGTA
    AATACCTTCA AATGCATTTG CAATTCCAGC TTTAGTGGGG TGCGCTGTGA GAAAAGCGAT
    GCAGCCTCAA TGTTCCCCTT TCCTCTTGTT GGAGTTGCAG TACCTGTAAC CTGTGGGGTT
    TTACTTCTAC TTGTTATTGG GGTCATATTC ATGGTGCTGA CAGCAAGGAA GAGGAGACAG
    TCAGAAGGAA CCTACAGCCC AAGCCAACAA GAAGTTGCAG GAGCACGACT TGAAATGGAC
    AGTGTTCTAA AGGTGCCTCC GGAGGAACGA TTGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031746761.1
    CDS87..4385
    Translation

    Target ORF information:

    RefSeq Version XM_031890901.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis crumbs 2, cell polarity complex component (crb2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031890901.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCAAGGAC TTGCCCAGAC CTGTGTGTCG TCCCCTTGCC TGAATGGTGG AACATGCCAG 
    GAAACAGCAA GTGGATTTAC CTGTCTGTGC CCAGCGGAGC CATTGGCATT TACTGGAACT
    ACCTGTGGAG ATCTGTATGA TGCCTGTGCC ATTGATAGAT GCCCCAGCAC CCATAGCTGT
    CAGAGCACCC CAGGGCATCC TCATTTTCAG TGTGTTTGCC AACCTGGGTT GAACTGCACC
    AATGAGTGTC AGAGAAATTC CTGCCCATTC CAAAATTTTC AGTGTACAGT TGGTGAGGAC
    AGCTTTATGT GCAAGTGCCA AAGTGGGTAC GGTGGAGTGG ACTGCAACTT TGAGGTATCA
    CCGTGTTTTC AGAACCCCTG CCAGAACAAT GCCACCTGTT TGGCAGAAGG AGACAGCTTC
    AGGTGTGTAT GTGAGCCAGG GTTCACAGGG CAGGAATGTG AGGTCAATGT GAACGAATGT
    GCTTCAAACC CATGCCAGAA CGAGGCTCTC TGTGTGGATA AAGTGGACAG ATACATGTGC
    TTCTGTGTGC CCGGCTTCCA AGGCCACCAT TGCGAAATAG ACATCAACGA GTGTGCTTCC
    AGACCATGTC AGAACAACGG AACTTGTATG AACCAACTGG ACCAATATGA ATGTGCTTGT
    GCCAATGGTT ACACAGGAAT TAACTGTGAG ATGGAGATAG ATGAATGTCA GTCAGTTCCA
    TGTCAGAATG GAGCTACATG TAGGGACCAT ATTGGCTACT TCACGTGTGA CTGCCCAGCG
    GGCTATGATG GAGAACAGTG CCAGCATGAC ATTGATGAGT GCCAGAGCCA GCCCTGTCAG
    AATGGAGGGC AGTGTATGGA TGAAATTAAC AGGTTCCGCT GTGATTGCAG TAATACTGGC
    TTTGTTGGTG GCCGCTGTGA AGTAGAAATC CTGGAGTGTG ACTCAAATCC TTGTCAGAAC
    AATGGCACGT GCATGGAGAG AGTCAATGGC TACGAATGCT TGTGCTGGAA AGGTTATTCT
    GGAATTCATT GTGAAATAGA TGATAATGAA TGTTTTGAGG AGCCATGTGT AAATGGTGGC
    CTATGTCTTG AACTATCCAA CCAGTCATAT TATGGCACTG AACCAGAATT TAACAAGTTC
    AGCTACAGCC AAGCAGCAGG ATATTTGTGC CGCTGCCAGC AAGGATTTAC AGGACCAAAC
    TGCTCTGTGA ACATTGATGA ATGTGAATCT TGGCCTTGTC TGAATGGCGG AACCTGCACC
    GACTTAATCA ATGGCTTCCT GTGTAACTGT GCTTCTGGAT ATACAGGTTT CGACTGTTCC
    ATAAACATCG ATGAGTGTAA AGACAGCCCA TGTAAGAATG GGGCAAGCTG TGAAGATGGA
    GTTGCTGACT ACTCCTGCAT CTGTCCACCA GCTGCCCAGG ATGGGACAGT ATGGGGTGGT
    AAAAACTGTT CTGTAATGTT GATTGGATGC TTGGAGCACA GCTGCCAAAA CGCAGCAGAG
    TGCATCCCTT TATATACTGG ACAAAGCCAC AGTTATACCT GCAAATGTCA ACCAGGTTTT
    GTTGGGAACA ATTGTTCAAC CCCTACAACA TTTTCCTTCT TGTCCCAGGG GTACCTAATG
    TATGATTCAC CTTTAAGTAA TGAATCGTAT CAAAGCAACA TGTCAGTGAG ATTCAGGACA
    ACATTACCTG ACATGGTCCT CATGTACCGA GGAGATGAAG ATAGCTTTCT CGCACTTGAG
    ATTTATAATG GATTTCTGCA CATGCGCCTT AAGGGAAATG GAAGGTTGTT TCCGCTGGTT
    ATTGATGATT ACAAGGTGAA CGATGGCCAC TGGCACAATG TGGAGGTAGA CCTTCTATCA
    AATTCATTCT TGAAGATCAG CCTTTTTCAT AATGATTGCC GTAATGGACT CTGCTCCCAA
    AAACAGACAC CCACTGAAGG ATTTGACCCC TATCTTCCAG AGTCCTTCAG ATCATTGTAT
    ATTGGAGGAC TTATTCAAGA GTCATTAATC AAGAACACTC TAAGTCAGCA AAACTTCACT
    GGCTGCCTGG AGGACTTGGT TGTTGACTTG GTTACTCAGC TGCCGCACAA CGTAAGCAGT
    GACAGGTCCT TTGGAATGGT ACTCGGATGC AAAAAGGCGG AGGTGTGCCA TCCAAACCCA
    TGTCACAATG GGGCAAACTG CATTGATCTC TGGTCCAGCT TTACATGTGA ATGCATCAGG
    CCCTTTAAAG GGTCCACCTG TATGCAGGAG TATATAGCAG GAACATTCTT CAGGGAGGGG
    ATTCCAAGCC ACTCTTACTT TGAACTCAAT CAGGATTTCG GAACGTCTTT CAATATATCT
    GCTTTTATCA GAACCCTAAA ATCGAATGGT CTCCTACTGC AAATCAGCTA CGGAACAACG
    CCTTACTTCA GAACTTACCT GAATAATAGT TGGATCCACA TTGAGACACT ATCTGCACAA
    CCTCTTGTCT TTAAGAAGAA CGTCAGTGAT GGCATGAAGC ACTTTATAAG TATTTCTGTC
    AGGGAAGGTA ACGTTACAGT CACTGATCTT CAGCAGGAAG TTTACTTGGG AATGTTGCCG
    CCGGTGATCA TTACCAAAGG AGATGCAGTC CATGTGGGCG GTTTGGCTAC AGCAGACAGT
    ACTATTTTTT GGGGTGGGTA TTTCAAAGGA TGTGTTCAAG ATTTAAGGAT TAACAACGAT
    CACTTGGAGT TCTTCCACCC AGAACATATA AATGTACAAG AAACCTTATA CTTTGGGAAC
    AACACCAACG TCACTGAGAA CTGCATCTCT GATGATGTTT GTCATCAAGG CCCATGCAGA
    AATGAAGGAA ACTGCTCAGT AACCTGGAAT GACTTTGTTT GCAGCTGTCC GGCCAACTTC
    ACCGGAAAAA GATGTGAGGA GCCGGTTTGG TGTCGACGCT CTCCCTGCTT GCCAGGCTCC
    ACTTGCGTGG ATGTTCCCAA AGGATTTGTG TGTCTGGCCA ATGTGAGCTT CCAGGGCCAC
    AGCTCTGCAG TATTCACTCC CATTATTTCA GAAGAACACA ATCTTACATC TATCTCATTG
    GCCTTCAGAA CCAGAGACAG AGATGCAGTT TTGCTCCAGT CTTCCAAAAA TGTGGATTCT
    GTGCTAATAG AAATTCAGGG GGGTTACTTG TTTGTTGATC TGCAAAGTGG AAATAATGTG
    GATGGAGTCC AGCTTAATGG AACAATAGAT GTTGCTGATG CTATCTGGCA CAGAGTAGTA
    ATAACCATGG AAAGAGCGAC TAAAGTGAAT TCTCATTGGA CATTCCAGCT GGATGACAGT
    GTTCAAAAGA AATTTCAGTG GGAAGGCGAC AACTTGAATT TTATGAGAAA TAACATTTCC
    ATTGTATTAG CTGAAGGCTA CACTGGATGT TTGGGCGATG TGTCAATTGG AGGGATTTAC
    CTTCCATTTA CAGACCATGT GTTGCCACAG CAGGATCAGT TTATAAGGAA ATCTGATGCT
    TTGGAGTTGG GATGCAGAGG GGCAGATGTT TGTTCTGAGA GTCCCTGTCT TCATGGTGGA
    ACATGCCAAG ATCTTTTTAA CTCCTTTAGC TGTACGTGCA GAATAGGGTG GAATGGGCAT
    CGTTGTGAGA TCAACATCGA TGACTGCAAG TCTAGCCCAT GTCTTCATGG TTTTTGTATA
    GACCTAGAGG CTGATTATCA GTGCAGCTGT GAGGCTGGTT ATACCAGCAG GAACTGCGAA
    ATCAACATCA ATGACTGTCA GCACCATCAG TGCCTGAATG GGGGGACTTG TGTTGATGGC
    GTAAATAACT ACACATGCAA GTGCCCAGCC AACTATGCGG GGACCTTCTG CCAATGGCCT
    TTTCCACCTG AGCAGTGCAA TAAAAACATC ACGTGTTTTA ATGGAGGCAG ATGCAACAGT
    GGAATTTGGG GAGCTAACTG CACATGTAGA CCAGGCTTTA GTGGCAGAAG GTGTGAAATA
    AATGTCAACG ATTGTGAATC AAACCCATGT CTCAATGGTG GAACTTGCCA GGATTCTGTA
    AATACCTTCA AATGCATTTG CAATTCCAGC TTTAGTGGGG TGCGCTGTGA GAAAAGCGAT
    GCAGCCTCAA TGTTCCCCTT TCCTCTTGTT GGAGTTGCAG TACCTGTAAC CTGTGGGGTT
    TTACTTCTAC TTGTTATTGG GGTCATATTC ATGGTGCTGA CAGCAAGGAA GAGGAGACAG
    TCAGAAGGAA CCTACAGCCC AAGCCAACAA GAAGTTGCAG GAGCACGACT TGAAATGGAC
    AGTGTTCTAA AGGTGCCTCC GGAGGAACGA TTGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa17941
    Clone ID Related Accession (Same CDS sequence) XM_002937234.5 , XM_002937234.4
    Accession Version XM_002937234.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein crumbs homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937234.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTACA GAAGCGTTAG TCGCACTTCA CCCAGTCCCG TTATTCTGCT ACTGCTACTT 
    CTCTTCTCCT GCGGGCAAGG ACTTGCCCAG ACCTGTGTGT CGTCCCCTTG CCTGAATGGT
    GGAACATGCC AGGAAACAGC AAGTGGATTT ACCTGTCTGT GCCCAGCGGA GCCATTGGCA
    TTTACTGGAA CTACCTGTGG AGATCTGTAT GATGCCTGTG CCATTGATAG ATGCCCCAGC
    ACCCATAGCT GTCAGAGCAC CCCAGGGCAT CCTCATTTTC AGTGTGTTTG CCAACCTGGG
    TTGAACTGCA CCAATGAGTG TCAGAGAAAT TCCTGCCCAT TCCAAAATTT TCAGTGTACA
    GTTGGTGAGG ACAGCTTTAT GTGCAAGTGC CAAAGTGGGT ACGGTGGAGT GGACTGCAAC
    TTTGAGGTAT CACCGTGTTT TCAGAACCCC TGCCAGAACA ATGCCACCTG TTTGGCAGAA
    GGAGACAGCT TCAGGTGTGT ATGTGAGCCA GGGTTCACAG GGCAGGAATG TGAGGTCAAT
    GTGAACGAAT GTGCTTCAAA CCCATGCCAG AACGAGGCTC TCTGTGTGGA TAAAGTGGAC
    AGATACATGT GCTTCTGTGT GCCCGGCTTC CAAGGCCACC ATTGCGAAAT AGACATCAAC
    GAGTGTGCTT CCAGACCATG TCAGAACAAC GGAACTTGTA TGAACCAACT GGACCAATAT
    GAATGTGCTT GTGCCAATGG TTACACAGGA ATTAACTGTG AGATGGAGAT AGATGAATGT
    CAGTCAGTTC CATGTCAGAA TGGAGCTACA TGTAGGGACC ATATTGGCTA CTTCACGTGT
    GACTGCCCAG CGGGCTATGA TGGAGAACAG TGCCAGCATG ACATTGATGA GTGCCAGAGC
    CAGCCCTGTC AGAATGGAGG GCAGTGTATG GATGAAATTA ACAGGTTCCG CTGTGATTGC
    AGTAATACTG GCTTTGTTGG TGGCCGCTGT GAAGTAGAAA TCCTGGAGTG TGACTCAAAT
    CCTTGTCAGA ACAATGGCAC GTGCATGGAG AGAGTCAATG GCTACGAATG CTTGTGCTGG
    AAAGGTTATT CTGGAATTCA TTGTGAAATA GATGATAATG AATGTTTTGA GGAGCCATGT
    GTAAATGGTG GCCTATGTCT TGAACTATCC AACCAGTCAT ATTATGGCAC TGAACCAGAA
    TTTAACAAGT TCAGCTACAG CCAAGCAGCA GGATATTTGT GCCGCTGCCA GCAAGGATTT
    ACAGGACCAA ACTGCTCTGT GAACATTGAT GAATGTGAAT CTTGGCCTTG TCTGAATGGC
    GGAACCTGCA CCGACTTAAT CAATGGCTTC CTGTGTAACT GTGCTTCTGG ATATACAGGT
    TTCGACTGTT CCATAAACAT CGATGAGTGT AAAGACAGCC CATGTAAGAA TGGGGCAAGC
    TGTGAAGATG GAGTTGCTGA CTACTCCTGC ATCTGTCCAC CAGCTGCCCA GGATGGGACA
    GTATGGGGTG GTAAAAACTG TTCTGTAATG TTGATTGGAT GCTTGGAGCA CAGCTGCCAA
    AACGCAGCAG AGTGCATCCC TTTATATACT GGACAAAGCC ACAGTTATAC CTGCAAATGT
    CAACCAGGTT TTGTTGGGAA CAATTGTTCA ACCCCTACAA CATTTTCCTT CTTGTCCCAG
    GGGTACCTAA TGTATGATTC ACCTTTAAGT AATGAATCGT ATCAAAGCAA CATGTCAGTG
    AGATTCAGGA CAACATTACC TGACATGGTC CTCATGTACC GAGGAGATGA AGATAGCTTT
    CTCGCACTTG AGATTTATAA TGGATTTCTG CACATGCGCC TTAAGGGAAA TGGAAGGTTG
    TTTCCGCTGG TTATTGATGA TTACAAGGTG AACGATGGCC ACTGGCACAA TGTGGAGGTA
    GACCTTCTAT CAAATTCATT CTTGAAGATC AGCCTTTTTC ATAATGATTG CCGTAATGGA
    CTCTGCTCCC AAAAACAGAC ACCCACTGAA GGATTTGACC CCTATCTTCC AGAGTCCTTC
    AGATCATTGT ATATTGGAGG ACTTATTCAA GAGTCATTAA TCAAGAACAC TCTAAGTCAG
    CAAAACTTCA CTGGCTGCCT GGAGGACTTG GTTGTTGACT TGGTTACTCA GCTGCCGCAC
    AACGTAAGCA GTGACAGGTC CTTTGGAATG GTACTCGGAT GCAAAAAGGC GGAGGTGTGC
    CATCCAAACC CATGTCACAA TGGGGCAAAC TGCATTGATC TCTGGTCCAG CTTTACATGT
    GAATGCATCA GGCCCTTTAA AGGGTCCACC TGTATGCAGG AGTATATAGC AGGAACATTC
    TTCAGGGAGG GGATTCCAAG CCACTCTTAC TTTGAACTCA ATCAGGATTT CGGAACGTCT
    TTCAATATAT CTGCTTTTAT CAGAACCCTA AAATCGAATG GTCTCCTACT GCAAATCAGC
    TACGGAACAA CGCCTTACTT CAGAACTTAC CTGAATAATA GTTGGATCCA CATTGAGACA
    CTATCTGCAC AACCTCTTGT CTTTAAGAAG AACGTCAGTG ATGGCATGAA GCACTTTATA
    AGTATTTCTG TCAGGGAAGG TAACGTTACA GTCACTGATC TTCAGCAGGA AGTTTACTTG
    GGAATGTTGC CGCCGGTGAT CATTACCAAA GGAGATGCAG TCCATGTGGG CGGTTTGGCT
    ACAGCAGACA GTACTATTTT TTGGGGTGGG TATTTCAAAG GATGTGTTCA AGATTTAAGG
    ATTAACAACG ATCACTTGGA GTTCTTCCAC CCAGAACATA TAAATGTACA AGAAACCTTA
    TACTTTGGGA ACAACACCAA CGTCACTGAG AACTGCATCT CTGATGATGT TTGTCATCAA
    GGCCCATGCA GAAATGAAGG AAACTGCTCA GTAACCTGGA ATGACTTTGT TTGCAGCTGT
    CCGGCCAACT TCACCGGAAA AAGATGTGAG GAGCCGGTTT GGTGTCGACG CTCTCCCTGC
    TTGCCAGGCT CCACTTGCGT GGATGTTCCC AAAGGATTTG TGTGTCTGGC CAATGTGAGC
    TTCCAGGGCC ACAGCTCTGC AGTATTCACT CCCATTATTT CAGAAGAACA CAATCTTACA
    TCTATCTCAT TGGCCTTCAG AACCAGAGAC AGAGATGCAG TTTTGCTCCA GTCTTCCAAA
    AATGTGGATT CTGTGCTAAT AGAAATTCAG GGGGGTTACT TGTTTGTTGA TCTGCAAAGT
    GGAAATAATG TGGATGGAGT CCAGCTTAAT GGAACAATAG ATGTTGCTGA TGCTATCTGG
    CACAGAGTAG TAATAACCAT GGAAAGAGCG ACTAAAGTGA ATTCTCATTG GACATTCCAG
    CTGGATGACA GTGTTCAAAA GAAATTTCAG TGGGAAGGCG ACAACTTGAA TTTTATGAGA
    AATAACATTT CCATTGTATT AGCTGAAGGC TACACTGGAT GTTTGGGCGA TGTGTCAATT
    GGAGGGATTT ACCTTCCATT TACAGACCAT GTGTTGCCAC AGCAGGATCA GTTTATAAGG
    AAATCTGATG CTTTGGAGTT GGGATGCAGA GGGGCAGATG TTTGTTCTGA GAGTCCCTGT
    CTTCATGGTG GAACATGCCA AGATCTTTTT AACTCCTTTA GCTGTACGTG CAGAATAGGG
    TGGAATGGGC ATCGTTGTGA GATCAACATC GATGACTGCA AGTCTAGCCC ATGTCTTCAT
    GGTTTTTGTA TAGACCTAGA GGCTGATTAT CAGTGCAGCT GTGAGGCTGG TTATACCAGC
    AGGAACTGCG AAATCAACAT CAATGACTGT CAGCACCATC AGTGCCTGAA TGGGGGGACT
    TGTGTTGATG GCGTAAATAA CTACACATGC AAGTGCCCAG CCAACTATGC GGGGACCTTC
    TGCCAATGGC CTTTTCCACC TGAGCAGTGC AATAAAAACA TCACGTGTTT TAATGGAGGC
    AGATGCAACA GTGGAATTTG GGGAGCTAAC TGCACATGTA GACCAGGCTT TAGTGGCAGA
    AGGTGTGAAA TAAATGTCAA CGATTGTGAA TCAAACCCAT GTCTCAATGG TGGAACTTGC
    CAGGATTCTG TAAATACCTT CAAATGCATT TGCAATTCCA GCTTTAGTGG GGTGCGCTGT
    GAGAAAAGCG ATGCAGCCTC AATGTTCCCC TTTCCTCTTG TTGGAGTTGC AGTACCTGTA
    ACCTGTGGGG TTTTACTTCT ACTTGTTATT GGGGTCATAT TCATGGTGCT GACAGCAAGG
    AAGAGGAGAC AGTCAGAAGG AACCTACAGC CCAAGCCAAC AAGAAGTTGC AGGAGCACGA
    CTTGAAATGG ACAGTGTTCT AAAGGTGCCT CCGGAGGAAC GATTGATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002937280.2
    CDS221..4591
    Translation

    Target ORF information:

    RefSeq Version XM_002937234.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis crumbs family member 2 (crb2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002937234.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTACA GAAGCGTTAG TCGCACTTCA CCCAGTCCCG TTATTCTGCT ACTGCTACTT 
    CTCTTCTCCT GCGGGCAAGG ACTTGCCCAG ACCTGTGTGT CGTCCCCTTG CCTGAATGGT
    GGAACATGCC AGGAAACAGC AAGTGGATTT ACCTGTCTGT GCCCAGCGGA GCCATTGGCA
    TTTACTGGAA CTACCTGTGG AGATCTGTAT GATGCCTGTG CCATTGATAG ATGCCCCAGC
    ACCCATAGCT GTCAGAGCAC CCCAGGGCAT CCTCATTTTC AGTGTGTTTG CCAACCTGGG
    TTGAACTGCA CCAATGAGTG TCAGAGAAAT TCCTGCCCAT TCCAAAATTT TCAGTGTACA
    GTTGGTGAGG ACAGCTTTAT GTGCAAGTGC CAAAGTGGGT ACGGTGGAGT GGACTGCAAC
    TTTGAGGTAT CACCGTGTTT TCAGAACCCC TGCCAGAACA ATGCCACCTG TTTGGCAGAA
    GGAGACAGCT TCAGGTGTGT ATGTGAGCCA GGGTTCACAG GGCAGGAATG TGAGGTCAAT
    GTGAACGAAT GTGCTTCAAA CCCATGCCAG AACGAGGCTC TCTGTGTGGA TAAAGTGGAC
    AGATACATGT GCTTCTGTGT GCCCGGCTTC CAAGGCCACC ATTGCGAAAT AGACATCAAC
    GAGTGTGCTT CCAGACCATG TCAGAACAAC GGAACTTGTA TGAACCAACT GGACCAATAT
    GAATGTGCTT GTGCCAATGG TTACACAGGA ATTAACTGTG AGATGGAGAT AGATGAATGT
    CAGTCAGTTC CATGTCAGAA TGGAGCTACA TGTAGGGACC ATATTGGCTA CTTCACGTGT
    GACTGCCCAG CGGGCTATGA TGGAGAACAG TGCCAGCATG ACATTGATGA GTGCCAGAGC
    CAGCCCTGTC AGAATGGAGG GCAGTGTATG GATGAAATTA ACAGGTTCCG CTGTGATTGC
    AGTAATACTG GCTTTGTTGG TGGCCGCTGT GAAGTAGAAA TCCTGGAGTG TGACTCAAAT
    CCTTGTCAGA ACAATGGCAC GTGCATGGAG AGAGTCAATG GCTACGAATG CTTGTGCTGG
    AAAGGTTATT CTGGAATTCA TTGTGAAATA GATGATAATG AATGTTTTGA GGAGCCATGT
    GTAAATGGTG GCCTATGTCT TGAACTATCC AACCAGTCAT ATTATGGCAC TGAACCAGAA
    TTTAACAAGT TCAGCTACAG CCAAGCAGCA GGATATTTGT GCCGCTGCCA GCAAGGATTT
    ACAGGACCAA ACTGCTCTGT GAACATTGAT GAATGTGAAT CTTGGCCTTG TCTGAATGGC
    GGAACCTGCA CCGACTTAAT CAATGGCTTC CTGTGTAACT GTGCTTCTGG ATATACAGGT
    TTCGACTGTT CCATAAACAT CGATGAGTGT AAAGACAGCC CATGTAAGAA TGGGGCAAGC
    TGTGAAGATG GAGTTGCTGA CTACTCCTGC ATCTGTCCAC CAGCTGCCCA GGATGGGACA
    GTATGGGGTG GTAAAAACTG TTCTGTAATG TTGATTGGAT GCTTGGAGCA CAGCTGCCAA
    AACGCAGCAG AGTGCATCCC TTTATATACT GGACAAAGCC ACAGTTATAC CTGCAAATGT
    CAACCAGGTT TTGTTGGGAA CAATTGTTCA ACCCCTACAA CATTTTCCTT CTTGTCCCAG
    GGGTACCTAA TGTATGATTC ACCTTTAAGT AATGAATCGT ATCAAAGCAA CATGTCAGTG
    AGATTCAGGA CAACATTACC TGACATGGTC CTCATGTACC GAGGAGATGA AGATAGCTTT
    CTCGCACTTG AGATTTATAA TGGATTTCTG CACATGCGCC TTAAGGGAAA TGGAAGGTTG
    TTTCCGCTGG TTATTGATGA TTACAAGGTG AACGATGGCC ACTGGCACAA TGTGGAGGTA
    GACCTTCTAT CAAATTCATT CTTGAAGATC AGCCTTTTTC ATAATGATTG CCGTAATGGA
    CTCTGCTCCC AAAAACAGAC ACCCACTGAA GGATTTGACC CCTATCTTCC AGAGTCCTTC
    AGATCATTGT ATATTGGAGG ACTTATTCAA GAGTCATTAA TCAAGAACAC TCTAAGTCAG
    CAAAACTTCA CTGGCTGCCT GGAGGACTTG GTTGTTGACT TGGTTACTCA GCTGCCGCAC
    AACGTAAGCA GTGACAGGTC CTTTGGAATG GTACTCGGAT GCAAAAAGGC GGAGGTGTGC
    CATCCAAACC CATGTCACAA TGGGGCAAAC TGCATTGATC TCTGGTCCAG CTTTACATGT
    GAATGCATCA GGCCCTTTAA AGGGTCCACC TGTATGCAGG AGTATATAGC AGGAACATTC
    TTCAGGGAGG GGATTCCAAG CCACTCTTAC TTTGAACTCA ATCAGGATTT CGGAACGTCT
    TTCAATATAT CTGCTTTTAT CAGAACCCTA AAATCGAATG GTCTCCTACT GCAAATCAGC
    TACGGAACAA CGCCTTACTT CAGAACTTAC CTGAATAATA GTTGGATCCA CATTGAGACA
    CTATCTGCAC AACCTCTTGT CTTTAAGAAG AACGTCAGTG ATGGCATGAA GCACTTTATA
    AGTATTTCTG TCAGGGAAGG TAACGTTACA GTCACTGATC TTCAGCAGGA AGTTTACTTG
    GGAATGTTGC CGCCGGTGAT CATTACCAAA GGAGATGCAG TCCATGTGGG CGGTTTGGCT
    ACAGCAGACA GTACTATTTT TTGGGGTGGG TATTTCAAAG GATGTGTTCA AGATTTAAGG
    ATTAACAACG ATCACTTGGA GTTCTTCCAC CCAGAACATA TAAATGTACA AGAAACCTTA
    TACTTTGGGA ACAACACCAA CGTCACTGAG AACTGCATCT CTGATGATGT TTGTCATCAA
    GGCCCATGCA GAAATGAAGG AAACTGCTCA GTAACCTGGA ATGACTTTGT TTGCAGCTGT
    CCGGCCAACT TCACCGGAAA AAGATGTGAG GAGCCGGTTT GGTGTCGACG CTCTCCCTGC
    TTGCCAGGCT CCACTTGCGT GGATGTTCCC AAAGGATTTG TGTGTCTGGC CAATGTGAGC
    TTCCAGGGCC ACAGCTCTGC AGTATTCACT CCCATTATTT CAGAAGAACA CAATCTTACA
    TCTATCTCAT TGGCCTTCAG AACCAGAGAC AGAGATGCAG TTTTGCTCCA GTCTTCCAAA
    AATGTGGATT CTGTGCTAAT AGAAATTCAG GGGGGTTACT TGTTTGTTGA TCTGCAAAGT
    GGAAATAATG TGGATGGAGT CCAGCTTAAT GGAACAATAG ATGTTGCTGA TGCTATCTGG
    CACAGAGTAG TAATAACCAT GGAAAGAGCG ACTAAAGTGA ATTCTCATTG GACATTCCAG
    CTGGATGACA GTGTTCAAAA GAAATTTCAG TGGGAAGGCG ACAACTTGAA TTTTATGAGA
    AATAACATTT CCATTGTATT AGCTGAAGGC TACACTGGAT GTTTGGGCGA TGTGTCAATT
    GGAGGGATTT ACCTTCCATT TACAGACCAT GTGTTGCCAC AGCAGGATCA GTTTATAAGG
    AAATCTGATG CTTTGGAGTT GGGATGCAGA GGGGCAGATG TTTGTTCTGA GAGTCCCTGT
    CTTCATGGTG GAACATGCCA AGATCTTTTT AACTCCTTTA GCTGTACGTG CAGAATAGGG
    TGGAATGGGC ATCGTTGTGA GATCAACATC GATGACTGCA AGTCTAGCCC ATGTCTTCAT
    GGTTTTTGTA TAGACCTAGA GGCTGATTAT CAGTGCAGCT GTGAGGCTGG TTATACCAGC
    AGGAACTGCG AAATCAACAT CAATGACTGT CAGCACCATC AGTGCCTGAA TGGGGGGACT
    TGTGTTGATG GCGTAAATAA CTACACATGC AAGTGCCCAG CCAACTATGC GGGGACCTTC
    TGCCAATGGC CTTTTCCACC TGAGCAGTGC AATAAAAACA TCACGTGTTT TAATGGAGGC
    AGATGCAACA GTGGAATTTG GGGAGCTAAC TGCACATGTA GACCAGGCTT TAGTGGCAGA
    AGGTGTGAAA TAAATGTCAA CGATTGTGAA TCAAACCCAT GTCTCAATGG TGGAACTTGC
    CAGGATTCTG TAAATACCTT CAAATGCATT TGCAATTCCA GCTTTAGTGG GGTGCGCTGT
    GAGAAAAGCG ATGCAGCCTC AATGTTCCCC TTTCCTCTTG TTGGAGTTGC AGTACCTGTA
    ACCTGTGGGG TTTTACTTCT ACTTGTTATT GGGGTCATAT TCATGGTGCT GACAGCAAGG
    AAGAGGAGAC AGTCAGAAGG AACCTACAGC CCAAGCCAAC AAGAAGTTGC AGGAGCACGA
    CTTGAAATGG ACAGTGTTCT AAAGGTGCCT CCGGAGGAAC GATTGATTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa17942
    Clone ID Related Accession (Same CDS sequence) XM_004918924.3 , XM_004918924.4
    Accession Version XM_004918924.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein crumbs homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004918924.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTACA GAAGCGTTAG TCGCACTTCA CCCAGTCCCG TTATTCTGCT ACTGCTACTT 
    CTCTTCTCCT GCGGGCAAGG ACTTGCCCAG ACCTGTGTGT CGTCCCCTTG CCTGAATGGT
    GGAACATGCC AGGAAACAGC AAGTGGATTT ACCTGTCTGT GCCCAGCGGA GCCATTGGCA
    TTTACTGGAA CTACCTGTGG AGATCTGTAT GATGCCTGTG CCATTGATAG ATGCCCCAGC
    ACCCATAGCT GTCAGAGCAC CCCAGGGCAT CCTCATTTTC AGTGTGTTTG CCAACCTGGG
    TTGAACTGCA CCAATGAGTG TCAGAGAAAT TCCTGCCCAT TCCAAAATTT TCAGTGTACA
    GTTGGTGAGG ACAGCTTTAT GTGCAAGTGC CAAAGTGGGT ACGGTGGAGT GGACTGCAAC
    TTTGAGGTAT CACCGTGTTT TCAGAACCCC TGCCAGAACA ATGCCACCTG TTTGGCAGAA
    GGAGACAGCT TCAGGTGTGT ATGTGAGCCA GGGTTCACAG GGCAGGAATG TGAGGTCAAT
    GTGAACGAAT GTGCTTCAAA CCCATGCCAG AACGAGGCTC TCTGTGTGGA TAAAGTGGAC
    AGATACATGT GCTTCTGTGT GCCCGGCTTC CAAGGCCACC ATTGCGAAAT AGACATCAAC
    GAGTGTGCTT CCAGACCATG TCAGAACAAC GGAACTTGTA TGAACCAACT GGACCAATAT
    GAATGTGCTT GTGCCAATGG TTACACAGGA ATTAACTGTG AGATGGAGAT AGATGAATGT
    CAGTCAGTTC CATGTCAGAA TGGAGCTACA TGTAGGGACC ATATTGGCTA CTTCACGTGT
    GACTGCCCAG CGGGCTATGA TGGAGAACAG TGCCAGCATG ACATTGATGA GTGCCAGAGC
    CAGCCCTGTC AGAATGGAGG GCAGTGTATG GATGAAATTA ACAGGTTCCG CTGTGATTGC
    AGTAATACTG GCTTTGTTGG TGGCCGCTGT GAAGTAGAAA TCCTGGAGTG TGACTCAAAT
    CCTTGTCAGA ACAATGGCAC GTGCATGGAG AGAGTCAATG GCTACGAATG CTTGTGCTGG
    AAAGGTTATT CTGGAATTCA TTGTGAAATA GATGATAATG AATGTTTTGA GGAGCCATGT
    GTAAATGGTG GCCTATGTCT TGAACTATCC AACCAGTCAT ATTATGGCAC TGAACCAGAA
    TTTAACAAGT TCAGCTACAG CCAAGCAGCA GGATATTTGT GCCGCTGCCA GCAAGGATTT
    ACAGGACCAA ACTGCTCTGT GAACATTGAT GAATGTGAAT CTTGGCCTTG TCTGAATGGC
    GGAACCTGCA CCGACTTAAT CAATGGCTTC CTGTGTAACT GTGCTTCTGG ATATACAGGT
    TTCGACTGTT CCATAAACAT CGATGAGTGT AAAGACAGCC CATGTAAGAA TGGGGCAAGC
    TGTGAAGATG GAGTTGCTGA CTACTCCTGC ATCTGTCCAC CAGCTGCCCA GGATGGGACA
    GTATGGGGTG GTAAAAACTG TTCTGTAATG TTGATTGGAT GCTTGGAGCA CAGCTGCCAA
    AACGCAGCAG AGTGCATCCC TTTATATACT GGACAAAGCC ACAGTTATAC CTGCAAATGT
    CAACCAGGTT TTGTTGGGAA CAATTGTTCA ACCCCTACAA CATTTTCCTT CTTGTCCCAG
    GGGTACCTAA TGTATGATTC ACCTTTAAGT AATGAATCGT ATCAAAGCAA CATGTCAGTG
    AGATTCAGGA CAACATTACC TGACATGGTC CTCATGTACC GAGGAGATGA AGATAGCTTT
    CTCGCACTTG AGATTTATAA TGGATTTCTG CACATGCGCC TTAAGGGAAA TGGAAGGTTG
    TTTCCGCTGG TTATTGATGA TTACAAGGTG AACGATGGCC ACTGGCACAA TGTGGAGGTA
    GACCTTCTAT CAAATTCATT CTTGAAGATC AGCCTTTTTC ATAATGATTG CCGTAATGGA
    CTCTGCTCCC AAAAACAGAC ACCCACTGAA GGATTTGACC CCTATCTTCC AGAGTCCTTC
    AGATCATTGT ATATTGGAGG ACTTATTCAA GAGTCATTAA TCAAGAACAC TCTAAGTCAG
    CAAAACTTCA CTGGCTGCCT GGAGGACTTG GTTGTTGACT TGGTTACTCA GCTGCCGCAC
    AACGTAAGCA GTGACAGGTC CTTTGGAATG GTACTCGGAT GCAAAAAGGC GGAGGTGTGC
    CATCCAAACC CATGTCACAA TGGGGCAAAC TGCATTGATC TCTGGTCCAG CTTTACATGT
    GAATGCATCA GGCCCTTTAA AGGGTCCACC TGTATGCAGG AGTATATAGC AGGAACATTC
    TTCAGGGAGG GGATTCCAAG CCACTCTTAC TTTGAACTCA ATCAGGATTT CGGAACGTCT
    TTCAATATAT CTGCTTTTAT CAGAACCCTA AAATCGAATG GTCTCCTACT GCAAATCAGC
    TACGGAACAA CGCCTTACTT CAGAACTTAC CTGAATAATA GTTGGATCCA CATTGAGACA
    CTATCTGCAC AACCTCTTGT CTTTAAGAAG AACGTCAGTG ATGGCATGAA GCACTTTATA
    AGTATTTCTG TCAGGGAAGG TAACGTTACA GTCACTGATC TTCAGCAGGA AGTTTACTTG
    GGAATGTTGC CGCCGGTGAT CATTACCAAA GGAGATGCAG TCCATGTGGG CGGTTTGGCT
    ACAGCAGACA GTACTATTTT TTGGGGTGGG TATTTCAAAG GATGTGTTCA AGATTTAAGG
    ATTAACAACG ATCACTTGGA GTTCTTCCAC CCAGAACATA TAAATGTACA AGAAACCTTA
    TACTTTGGGA ACAACACCAA CGTCACTGAG AACTGCATCT CTGATGATGT TTGTCATCAA
    GGCCCATGCA GAAATGAAGG AAACTGCTCA GTAACCTGGA ATGACTTTGT TTGCAGCTGT
    CCGGCCAACT TCACCGGAAA AAGATGTGAG GAGCCGGTTT GGTGTCGACG CTCTCCCTGC
    TTGCCAGGCT CCACTTGCGT GGATGTTCCC AAAGGATTTG TGTGTCTGGC CAATGTGAGC
    TTCCAGGGCC ACAGCTCTGC AGTATTCACT CCCATTATTT CAGAAGAACA CAATCTTACA
    TCTATCTCAT TGGCCTTCAG AACCAGAGAC AGAGATGCAG TTTTGCTCCA GTCTTCCAAA
    AATGTGGATT CTGTGCTAAT AGAAATTCAG GGGGGTTACT TGTTTGTTGA TCTGCAAAGT
    GGAAATAATG TGGATGGAGT CCAGCTTAAT GGAACAATAG ATGTTGCTGA TGCTATCTGG
    CACAGAGTAG TAATAACCAT GGAAAGAGCG ACTAAAGTGA ATTCTCATTG GACATTCCAG
    CTGGATGACA GTGTTCAAAA GAAATTTCAG TGGGAAGGCG ACAACTTGAA TTTTATGAGA
    AATAACATTT CCATTGTATT AGCTGAAGGC TACACTGGAT GTTTGGGCGA TGTGTCAATT
    GGAGGGATTT ACCTTCCATT TACAGACCAT GTGTTGCCAC AGCAGGATCA GTTTATAAGG
    AAATCTGATG CTTTGGAGTT GGGATGCAGA GGGGCAGATG TTTGTTCTGA GAGTCCCTGT
    CTTCATGGTG GAACATGCCA AGATCTTTTT AACTCCTTTA GCTGTACGTG CAGAATAGGG
    TGGAATGGGC ATCGTTGTGA GATCAACATC GATGACTGCA AGTCTAGCCC ATGTCTTCAT
    GGTTTTTGTA TAGACCTAGA GGCTGATTAT CAGTGCAGCT GTGAGGCTGG TTATACCAGC
    AGGAACTGCG AAATCAACAT CAATGACTGT CAGCACCATC AGTGCCTGAA TGGGGGGACT
    TGTGTTGATG GCGTAAATAA CTACACATGC AAGTGCCCAG CCAACTATGC GGGGACCTTC
    TGCCAATGGC CTTTTCCACC TGAGCAGTGC AATAAAAACA TCACGTGTTT TAATGGAGGC
    AGATGCAACA GTGGAATTTG GGGAGCTAAC TGCACATGTA GACCAGGCTT TAGTGGCAGA
    AGGTGTGAAA TAAATGTCAA CGATTGTGAA TCAAACCCAT GTCTCAATGG TGGAACTTGC
    CAGGATTCTG TAAATACCTT CAAATGCATT TGCAATTCCA GCTTTAGTGG GGTGCGCTGT
    GAGAAGCCAC GCCTGCACCG TGAAGCAAAG GCCTCCACTC TGGTTGGGTC GGCAATAGGA
    ACAGGAATGC TCTTCATACT GCTTTCTGTC ATCACTGCAG TTTTGGTGAC TATGAGAAAT
    AAGAGAGCAA CTCAGGGGAC TTACAGTCCC AGCCGCCAGG AGAAGGATGG GGCTCGAGTG
    GAAATGTGGA ATGTCCTGAA GCTTCCACCC ACCGAGCGTC TTATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004918981.1
    CDS223..4590
    Translation

    Target ORF information:

    RefSeq Version XM_004918924.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis crumbs family member 2 (crb2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004918924.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGACTACA GAAGCGTTAG TCGCACTTCA CCCAGTCCCG TTATTCTGCT ACTGCTACTT 
    CTCTTCTCCT GCGGGCAAGG ACTTGCCCAG ACCTGTGTGT CGTCCCCTTG CCTGAATGGT
    GGAACATGCC AGGAAACAGC AAGTGGATTT ACCTGTCTGT GCCCAGCGGA GCCATTGGCA
    TTTACTGGAA CTACCTGTGG AGATCTGTAT GATGCCTGTG CCATTGATAG ATGCCCCAGC
    ACCCATAGCT GTCAGAGCAC CCCAGGGCAT CCTCATTTTC AGTGTGTTTG CCAACCTGGG
    TTGAACTGCA CCAATGAGTG TCAGAGAAAT TCCTGCCCAT TCCAAAATTT TCAGTGTACA
    GTTGGTGAGG ACAGCTTTAT GTGCAAGTGC CAAAGTGGGT ACGGTGGAGT GGACTGCAAC
    TTTGAGGTAT CACCGTGTTT TCAGAACCCC TGCCAGAACA ATGCCACCTG TTTGGCAGAA
    GGAGACAGCT TCAGGTGTGT ATGTGAGCCA GGGTTCACAG GGCAGGAATG TGAGGTCAAT
    GTGAACGAAT GTGCTTCAAA CCCATGCCAG AACGAGGCTC TCTGTGTGGA TAAAGTGGAC
    AGATACATGT GCTTCTGTGT GCCCGGCTTC CAAGGCCACC ATTGCGAAAT AGACATCAAC
    GAGTGTGCTT CCAGACCATG TCAGAACAAC GGAACTTGTA TGAACCAACT GGACCAATAT
    GAATGTGCTT GTGCCAATGG TTACACAGGA ATTAACTGTG AGATGGAGAT AGATGAATGT
    CAGTCAGTTC CATGTCAGAA TGGAGCTACA TGTAGGGACC ATATTGGCTA CTTCACGTGT
    GACTGCCCAG CGGGCTATGA TGGAGAACAG TGCCAGCATG ACATTGATGA GTGCCAGAGC
    CAGCCCTGTC AGAATGGAGG GCAGTGTATG GATGAAATTA ACAGGTTCCG CTGTGATTGC
    AGTAATACTG GCTTTGTTGG TGGCCGCTGT GAAGTAGAAA TCCTGGAGTG TGACTCAAAT
    CCTTGTCAGA ACAATGGCAC GTGCATGGAG AGAGTCAATG GCTACGAATG CTTGTGCTGG
    AAAGGTTATT CTGGAATTCA TTGTGAAATA GATGATAATG AATGTTTTGA GGAGCCATGT
    GTAAATGGTG GCCTATGTCT TGAACTATCC AACCAGTCAT ATTATGGCAC TGAACCAGAA
    TTTAACAAGT TCAGCTACAG CCAAGCAGCA GGATATTTGT GCCGCTGCCA GCAAGGATTT
    ACAGGACCAA ACTGCTCTGT GAACATTGAT GAATGTGAAT CTTGGCCTTG TCTGAATGGC
    GGAACCTGCA CCGACTTAAT CAATGGCTTC CTGTGTAACT GTGCTTCTGG ATATACAGGT
    TTCGACTGTT CCATAAACAT CGATGAGTGT AAAGACAGCC CATGTAAGAA TGGGGCAAGC
    TGTGAAGATG GAGTTGCTGA CTACTCCTGC ATCTGTCCAC CAGCTGCCCA GGATGGGACA
    GTATGGGGTG GTAAAAACTG TTCTGTAATG TTGATTGGAT GCTTGGAGCA CAGCTGCCAA
    AACGCAGCAG AGTGCATCCC TTTATATACT GGACAAAGCC ACAGTTATAC CTGCAAATGT
    CAACCAGGTT TTGTTGGGAA CAATTGTTCA ACCCCTACAA CATTTTCCTT CTTGTCCCAG
    GGGTACCTAA TGTATGATTC ACCTTTAAGT AATGAATCGT ATCAAAGCAA CATGTCAGTG
    AGATTCAGGA CAACATTACC TGACATGGTC CTCATGTACC GAGGAGATGA AGATAGCTTT
    CTCGCACTTG AGATTTATAA TGGATTTCTG CACATGCGCC TTAAGGGAAA TGGAAGGTTG
    TTTCCGCTGG TTATTGATGA TTACAAGGTG AACGATGGCC ACTGGCACAA TGTGGAGGTA
    GACCTTCTAT CAAATTCATT CTTGAAGATC AGCCTTTTTC ATAATGATTG CCGTAATGGA
    CTCTGCTCCC AAAAACAGAC ACCCACTGAA GGATTTGACC CCTATCTTCC AGAGTCCTTC
    AGATCATTGT ATATTGGAGG ACTTATTCAA GAGTCATTAA TCAAGAACAC TCTAAGTCAG
    CAAAACTTCA CTGGCTGCCT GGAGGACTTG GTTGTTGACT TGGTTACTCA GCTGCCGCAC
    AACGTAAGCA GTGACAGGTC CTTTGGAATG GTACTCGGAT GCAAAAAGGC GGAGGTGTGC
    CATCCAAACC CATGTCACAA TGGGGCAAAC TGCATTGATC TCTGGTCCAG CTTTACATGT
    GAATGCATCA GGCCCTTTAA AGGGTCCACC TGTATGCAGG AGTATATAGC AGGAACATTC
    TTCAGGGAGG GGATTCCAAG CCACTCTTAC TTTGAACTCA ATCAGGATTT CGGAACGTCT
    TTCAATATAT CTGCTTTTAT CAGAACCCTA AAATCGAATG GTCTCCTACT GCAAATCAGC
    TACGGAACAA CGCCTTACTT CAGAACTTAC CTGAATAATA GTTGGATCCA CATTGAGACA
    CTATCTGCAC AACCTCTTGT CTTTAAGAAG AACGTCAGTG ATGGCATGAA GCACTTTATA
    AGTATTTCTG TCAGGGAAGG TAACGTTACA GTCACTGATC TTCAGCAGGA AGTTTACTTG
    GGAATGTTGC CGCCGGTGAT CATTACCAAA GGAGATGCAG TCCATGTGGG CGGTTTGGCT
    ACAGCAGACA GTACTATTTT TTGGGGTGGG TATTTCAAAG GATGTGTTCA AGATTTAAGG
    ATTAACAACG ATCACTTGGA GTTCTTCCAC CCAGAACATA TAAATGTACA AGAAACCTTA
    TACTTTGGGA ACAACACCAA CGTCACTGAG AACTGCATCT CTGATGATGT TTGTCATCAA
    GGCCCATGCA GAAATGAAGG AAACTGCTCA GTAACCTGGA ATGACTTTGT TTGCAGCTGT
    CCGGCCAACT TCACCGGAAA AAGATGTGAG GAGCCGGTTT GGTGTCGACG CTCTCCCTGC
    TTGCCAGGCT CCACTTGCGT GGATGTTCCC AAAGGATTTG TGTGTCTGGC CAATGTGAGC
    TTCCAGGGCC ACAGCTCTGC AGTATTCACT CCCATTATTT CAGAAGAACA CAATCTTACA
    TCTATCTCAT TGGCCTTCAG AACCAGAGAC AGAGATGCAG TTTTGCTCCA GTCTTCCAAA
    AATGTGGATT CTGTGCTAAT AGAAATTCAG GGGGGTTACT TGTTTGTTGA TCTGCAAAGT
    GGAAATAATG TGGATGGAGT CCAGCTTAAT GGAACAATAG ATGTTGCTGA TGCTATCTGG
    CACAGAGTAG TAATAACCAT GGAAAGAGCG ACTAAAGTGA ATTCTCATTG GACATTCCAG
    CTGGATGACA GTGTTCAAAA GAAATTTCAG TGGGAAGGCG ACAACTTGAA TTTTATGAGA
    AATAACATTT CCATTGTATT AGCTGAAGGC TACACTGGAT GTTTGGGCGA TGTGTCAATT
    GGAGGGATTT ACCTTCCATT TACAGACCAT GTGTTGCCAC AGCAGGATCA GTTTATAAGG
    AAATCTGATG CTTTGGAGTT GGGATGCAGA GGGGCAGATG TTTGTTCTGA GAGTCCCTGT
    CTTCATGGTG GAACATGCCA AGATCTTTTT AACTCCTTTA GCTGTACGTG CAGAATAGGG
    TGGAATGGGC ATCGTTGTGA GATCAACATC GATGACTGCA AGTCTAGCCC ATGTCTTCAT
    GGTTTTTGTA TAGACCTAGA GGCTGATTAT CAGTGCAGCT GTGAGGCTGG TTATACCAGC
    AGGAACTGCG AAATCAACAT CAATGACTGT CAGCACCATC AGTGCCTGAA TGGGGGGACT
    TGTGTTGATG GCGTAAATAA CTACACATGC AAGTGCCCAG CCAACTATGC GGGGACCTTC
    TGCCAATGGC CTTTTCCACC TGAGCAGTGC AATAAAAACA TCACGTGTTT TAATGGAGGC
    AGATGCAACA GTGGAATTTG GGGAGCTAAC TGCACATGTA GACCAGGCTT TAGTGGCAGA
    AGGTGTGAAA TAAATGTCAA CGATTGTGAA TCAAACCCAT GTCTCAATGG TGGAACTTGC
    CAGGATTCTG TAAATACCTT CAAATGCATT TGCAATTCCA GCTTTAGTGG GGTGCGCTGT
    GAGAAGCCAC GCCTGCACCG TGAAGCAAAG GCCTCCACTC TGGTTGGGTC GGCAATAGGA
    ACAGGAATGC TCTTCATACT GCTTTCTGTC ATCACTGCAG TTTTGGTGAC TATGAGAAAT
    AAGAGAGCAA CTCAGGGGAC TTACAGTCCC AGCCGCCAGG AGAAGGATGG GGCTCGAGTG
    GAAATGTGGA ATGTCCTGAA GCTTCCACCC ACCGAGCGTC TTATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.