lgr5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	lgr5
Entrez Gene ID	100486389
Full Name	leucine-rich repeat containing G protein-coupled receptor 5
General protein information	Preferred Names leucine-rich repeat containing G protein-coupled receptor 5 Names leucine-rich repeat-containing G-protein coupled receptor 5
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002940173.5	XP_002940219.2	leucine-rich repeat-containing G-protein coupled receptor 5
XM_002940173.4	XP_002940219.2	leucine-rich repeat-containing G-protein coupled receptor 5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LGR5	XP_003313909.1
	LGR5	XP_001117502.1
Drosophila melanogaster (fruit fly)	rk	NP_476702.1
Mus musculus (house mouse)	Lgr5	NP_034325.2
Homo sapiens (human)	LGR5	NP_003658.1
Gallus gallus (chicken)	LGR5	XP_425441.2
Rattus norvegicus (Norway rat)	Lgr5	NP_001100254.1
Bos taurus (cattle)	LGR5	NP_001179449.1
Anopheles gambiae (African malaria mosquito)	GPRRK	XP_317111.1
Xenopus tropicalis (tropical clawed frog)	lgr5	XP_002940219.2

Gene Ontology

The following lgr5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lgr5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa07769	XM_002940173.5 Latest version!	Xenopus tropicalis leucine-rich repeat containing G protein-coupled receptor 5 (lgr5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa07769	XM_002940173.4	Xenopus tropicalis leucine-rich repeat containing G protein-coupled receptor 5 (lgr5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lgr5 ( XM_002940173.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940219.2 Xenopus tropicalis leucine-rich repeat containing G protein-coupled receptor 5 (lgr5), mRNA.

Price & Availability↑

CloneID	OXa07769
Clone ID Related Accession (Same CDS sequence)	XM_002940173.5 , XM_002940173.4
Accession Version	XM_002940173.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	leucine-rich repeat-containing G-protein coupled receptor 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940173.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCT CCAAGACCAG CTTCTTCTTG TTCTCGGTGC TGTGCAGCTT GCAATTGGTC 
GGCGCTGCTC GGCCTGGCAA ACAGCAGAGA AGCTGCCCGA CTCCATGTGA GTGCGAACAG 
GAGGGGATGT TGGTGCGAGT GGACTGCTCG GATCGGGCAC TGACTAGCCT GCCCAGGAAC 
CTCAGCATCT TCACGTCTTA CCTTGATCTA AGTATGAACA ACATTACCAA TCTGCCTTCC 
AATGTAATGC ACAACCTGCA CTTCTTAGAG GAGCTACGTC TTGCAGGAAA TGACTTGACA 
TACATCCCCA AGGGAGCTTT TGCTGGGCTC GGCAGCCTAA AAGTTTTAAT GCTACAGAAC 
AACCTGTTAA GGCAGGTACC GAGTGAAGCT CTTCACAACC TGCGCAGCCT TCAGTCTCTG 
CGCTTGGATG CAAACCATAT CAGCTATGTT CCACCAAGCT CTTTCAATGG TTTATTTTCC 
CTCCGTCACC TCTGGCTTGA TGACAATTCT TTGACTGAGA TTCCCGTGAG AGCACTTGAA 
AGCTTGTCTG CATTACAGGC TATGACCTTG GCACTAAACA AGATTCATCA TATACCAGAT 
TATGCCTTCA GAAACCTTTC AAGTCTGGTA GTTCTCCATC TCCATAACAA TAGAATCTAC 
TCCCTGGGAA AGAAATGCTT TGACGGGCTC CACAGTCTGG AGACTTTGGA CTTGAACTAC 
AATAATTTAG ATGAATTTCC TGCTGCCATA AAAACCCTAA AGAACCTTAA AGAATTGGGA 
TTTCATAGTA ACAACATCAA ATCGATCCCT GAGCAAGCAT TCATTGGCAA CCCTTCTCTA 
ATCACAATTC ACTTTTATGA CAACCCTATT CAACATGTGG GAAGATCAGC CTTTCAACAT 
TTGCCTGAGC TTAGAACTTT GATTCTTAAT GGGGCTTCAC AAATCACAGA ATTTCCTGAT 
CTAACTGGAA CTACAAGCCT GGAGAGTTTG ACTCTTACAG GAGCGCAGCT TGTGTACCTT 
CCCAGCGCTG TCTGCACTCA GCTGCCCAAC CTCCAAGTGC TGGACTTGTC ATATAATCAC 
ATCAAGGATT TGCCAAGCTT CTCCGGTTGT CAGCGACTGC AGAAAATAGA TCTGCGTCAT 
AACGAAGTCT ATGAAATTAG ATCCACCACC TTTCAGCAGC TGGTTGGCCT TCGTTCTCTA 
GACTTGGCAT GGAACAAGAT TGCTGTCATC CATCCTAACT CTTTTTCTTC TTTGCCTTCC 
TTAATCAAAT TGGATCTGTC ATCCAATCAT TTGACCTCAT TCCCTGTGAC CGGCCTTCAT 
GGTTTAACTC ATCTAAAGCT AACAGGAAAC TCAGCCCTTC AGGATCTGAT CCCATCTGAA 
CATTTCCCAA AGCTCAGGGT CATGGAGATG CCCTATGCCT ACCAGTGTTG TGCCTTTGCG 
GTTTGTGACA ATCTTAAGCA TTCAGGCCAG ATGAACAAAG ATGAAAACAG TAGTGCTGAT 
GATTTCTATC GCAAAGACAT AGGGCTGCTG CACCTTCAAG ATGACAGAGA CTTTGAAGAT 
TTTTTGCTTG ATTTTGAAGA AGATGTAAAA GTGCTGCATT CAGTACAGTG TACACCATCA 
GCAGGACCTT TCAAGCCTTG TGATCACCTT TTTGGAAGTT GGCTGACCAG AACTGGAGTG 
TGGCTTATAG TCCTCCTATC TTTTGTTTGT AATGCTTTGG TGATTGCAAC AGTCTTCCGA 
CCTCTGTCAT ATGTCCCTTC CATAAAGCTT CTCATTGGCC TAATAGCCAT TATGAATACA 
CTGATGGGCT TGTCCAGTGG TGTGCTAGCG ACTGTTGATG CCTTAACCTT TGGCAACTTT 
GCTCAGTATG GTGCCTGGTG GGAAAGTGGT GTAGGTTGCC AGATAACTGG GTTTCTGTCA 
GTTTTTGCAG CGGAGACCTC AATCTTTCTC CTTACTGTTG CTGCATTAGA GCGGGGGTTC 
TCCATTAAAT GTACTACAAA ATTTGAAACA AAGTCTTCAT TTATAAATGT CAAACTTTCC 
ATTGTCTTTT GCTTCCTTCT ATCCATTGTT ATTGCAGTTT CACCTTTGCT GAGTGGTAGT 
ACCTATGGGA CTTCTCCGTT GTGCTTTCCC CTGCTTTTTG GGGATCCCTC CTCTATGGGC 
TTTATGGTAG CTTTGGTTCT TCTAAACTCA TTATGCTTTC TTGTAATGAC TATTGCCTAT 
ACAAAACTCT ACTGCTCTCT AGAAAAAGGC GAATTGGAAA ATATATGGGA CTGCTCAATG 
GTGAAACATA TTGCCTTGCT GCTCTTCACA AACTGCATCC TATACTGCCC TGTAGCCTTT 
CTGTCTTTTT CCTCACTGTT AAATCTAACA TTCATCAGCC CTGAAGTGAA TAAGTCAATA 
CTCTTGTTGA TCATTCCACT CCCTGCATGT TTAAACCCTC TGCTATACAT CCTTTTTAAT 
CCACATTTCA AAGAAGATAT CGGAAGTCTG AAAAACGGAG ATATCCTGTG GAGTAGATCT 
AGACAAACGA GTTTGGCCTC AGTCAGTTCT GAAGATGCTG AAAAGCAGTC CTGTGATTCT 
ACACAAGCCC TTGTAACCTT TGCAAACTCC AGCATATCAT ATGATTTGCC AGCAACCAGC 
TCCTCATCAT CCTATCAGAT GACTAACAAC TATAAACTTT CAGCAGTGGC ATTTGTTCCT 
TGTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940219.2
CDS	282..2990
Translation	MDTSKTSFFLFSVLCSLQLVGAARPGKQQRSCPTPCECEQEGMLVRVDCSDRALTSLPRNLSIFTSYLDLSMNNITNLPSNVMHNLHFLEELRLAGNDLTYIPKGAFAGLGSLKVLMLQNNLLRQVPSEALHNLRSLQSLRLDANHISYVPPSSFNGLFSLRHLWLDDNSLTEIPVRALESLSALQAMTLALNKIHHIPDYAFRNLSSLVVLHLHNNRIYSLGKKCFDGLHSLETLDLNYNNLDEFPAAIKTLKNLKELGFHSNNIKSIPEQAFIGNPSLITIHFYDNPIQHVGRSAFQHLPELRTLILNGASQITEFPDLTGTTSLESLTLTGAQLVYLPSAVCTQLPNLQVLDLSYNHIKDLPSFSGCQRLQKIDLRHNEVYEIRSTTFQQLVGLRSLDLAWNKIAVIHPNSFSSLPSLIKLDLSSNHLTSFPVTGLHGLTHLKLTGNSALQDLIPSEHFPKLRVMEMPYAYQCCAFAVCDNLKHSGQMNKDENSSADDFYRKDIGLLHLQDDRDFEDFLLDFEEDVKVLHSVQCTPSAGPFKPCDHLFGSWLTRTGVWLIVLLSFVCNALVIATVFRPLSYVPSIKLLIGLIAIMNTLMGLSSGVLATVDALTFGNFAQYGAWWESGVGCQITGFLSVFAAETSIFLLTVAALERGFSIKCTTKFETKSSFINVKLSIVFCFLLSIVIAVSPLLSGSTYGTSPLCFPLLFGDPSSMGFMVALVLLNSLCFLVMTIAYTKLYCSLEKGELENIWDCSMVKHIALLLFTNCILYCPVAFLSFSSLLNLTFISPEVNKSILLLIIPLPACLNPLLYILFNPHFKEDIGSLKNGDILWSRSRQTSLASVSSEDAEKQSCDSTQALVTFANSSISYDLPATSSSSSYQMTNNYKLSAVAFVPCH

Target ORF information:

RefSeq Version	XM_002940173.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis leucine-rich repeat containing G protein-coupled receptor 5 (lgr5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940173.4

ORF Insert Sequence:

ATGGACACCT CCAAGACCAG CTTCTTCTTG TTCTCGGTGC TGTGCAGCTT GCAATTGGTC 
GGCGCTGCTC GGCCTGGCAA ACAGCAGAGA AGCTGCCCGA CTCCATGTGA GTGCGAACAG 
GAGGGGATGT TGGTGCGAGT GGACTGCTCG GATCGGGCAC TGACTAGCCT GCCCAGGAAC 
CTCAGCATCT TCACGTCTTA CCTTGATCTA AGTATGAACA ACATTACCAA TCTGCCTTCC 
AATGTAATGC ACAACCTGCA CTTCTTAGAG GAGCTACGTC TTGCAGGAAA TGACTTGACA 
TACATCCCCA AGGGAGCTTT TGCTGGGCTC GGCAGCCTAA AAGTTTTAAT GCTACAGAAC 
AACCTGTTAA GGCAGGTACC GAGTGAAGCT CTTCACAACC TGCGCAGCCT TCAGTCTCTG 
CGCTTGGATG CAAACCATAT CAGCTATGTT CCACCAAGCT CTTTCAATGG TTTATTTTCC 
CTCCGTCACC TCTGGCTTGA TGACAATTCT TTGACTGAGA TTCCCGTGAG AGCACTTGAA 
AGCTTGTCTG CATTACAGGC TATGACCTTG GCACTAAACA AGATTCATCA TATACCAGAT 
TATGCCTTCA GAAACCTTTC AAGTCTGGTA GTTCTCCATC TCCATAACAA TAGAATCTAC 
TCCCTGGGAA AGAAATGCTT TGACGGGCTC CACAGTCTGG AGACTTTGGA CTTGAACTAC 
AATAATTTAG ATGAATTTCC TGCTGCCATA AAAACCCTAA AGAACCTTAA AGAATTGGGA 
TTTCATAGTA ACAACATCAA ATCGATCCCT GAGCAAGCAT TCATTGGCAA CCCTTCTCTA 
ATCACAATTC ACTTTTATGA CAACCCTATT CAACATGTGG GAAGATCAGC CTTTCAACAT 
TTGCCTGAGC TTAGAACTTT GATTCTTAAT GGGGCTTCAC AAATCACAGA ATTTCCTGAT 
CTAACTGGAA CTACAAGCCT GGAGAGTTTG ACTCTTACAG GAGCGCAGCT TGTGTACCTT 
CCCAGCGCTG TCTGCACTCA GCTGCCCAAC CTCCAAGTGC TGGACTTGTC ATATAATCAC 
ATCAAGGATT TGCCAAGCTT CTCCGGTTGT CAGCGACTGC AGAAAATAGA TCTGCGTCAT 
AACGAAGTCT ATGAAATTAG ATCCACCACC TTTCAGCAGC TGGTTGGCCT TCGTTCTCTA 
GACTTGGCAT GGAACAAGAT TGCTGTCATC CATCCTAACT CTTTTTCTTC TTTGCCTTCC 
TTAATCAAAT TGGATCTGTC ATCCAATCAT TTGACCTCAT TCCCTGTGAC CGGCCTTCAT 
GGTTTAACTC ATCTAAAGCT AACAGGAAAC TCAGCCCTTC AGGATCTGAT CCCATCTGAA 
CATTTCCCAA AGCTCAGGGT CATGGAGATG CCCTATGCCT ACCAGTGTTG TGCCTTTGCG 
GTTTGTGACA ATCTTAAGCA TTCAGGCCAG ATGAACAAAG ATGAAAACAG TAGTGCTGAT 
GATTTCTATC GCAAAGACAT AGGGCTGCTG CACCTTCAAG ATGACAGAGA CTTTGAAGAT 
TTTTTGCTTG ATTTTGAAGA AGATGTAAAA GTGCTGCATT CAGTACAGTG TACACCATCA 
GCAGGACCTT TCAAGCCTTG TGATCACCTT TTTGGAAGTT GGCTGACCAG AACTGGAGTG 
TGGCTTATAG TCCTCCTATC TTTTGTTTGT AATGCTTTGG TGATTGCAAC AGTCTTCCGA 
CCTCTGTCAT ATGTCCCTTC CATAAAGCTT CTCATTGGCC TAATAGCCAT TATGAATACA 
CTGATGGGCT TGTCCAGTGG TGTGCTAGCG ACTGTTGATG CCTTAACCTT TGGCAACTTT 
GCTCAGTATG GTGCCTGGTG GGAAAGTGGT GTAGGTTGCC AGATAACTGG GTTTCTGTCA 
GTTTTTGCAG CGGAGACCTC AATCTTTCTC CTTACTGTTG CTGCATTAGA GCGGGGGTTC 
TCCATTAAAT GTACTACAAA ATTTGAAACA AAGTCTTCAT TTATAAATGT CAAACTTTCC 
ATTGTCTTTT GCTTCCTTCT ATCCATTGTT ATTGCAGTTT CACCTTTGCT GAGTGGTAGT 
ACCTATGGGA CTTCTCCGTT GTGCTTTCCC CTGCTTTTTG GGGATCCCTC CTCTATGGGC 
TTTATGGTAG CTTTGGTTCT TCTAAACTCA TTATGCTTTC TTGTAATGAC TATTGCCTAT 
ACAAAACTCT ACTGCTCTCT AGAAAAAGGC GAATTGGAAA ATATATGGGA CTGCTCAATG 
GTGAAACATA TTGCCTTGCT GCTCTTCACA AACTGCATCC TATACTGCCC TGTAGCCTTT 
CTGTCTTTTT CCTCACTGTT AAATCTAACA TTCATCAGCC CTGAAGTGAA TAAGTCAATA 
CTCTTGTTGA TCATTCCACT CCCTGCATGT TTAAACCCTC TGCTATACAT CCTTTTTAAT 
CCACATTTCA AAGAAGATAT CGGAAGTCTG AAAAACGGAG ATATCCTGTG GAGTAGATCT 
AGACAAACGA GTTTGGCCTC AGTCAGTTCT GAAGATGCTG AAAAGCAGTC CTGTGATTCT 
ACACAAGCCC TTGTAACCTT TGCAAACTCC AGCATATCAT ATGATTTGCC AGCAACCAGC 
TCCTCATCAT CCTATCAGAT GACTAACAAC TATAAACTTT CAGCAGTGGC ATTTGTTCCT 
TGTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lgr5 ( XM_002940173.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940219.2 Xenopus tropicalis leucine-rich repeat containing G protein-coupled receptor 5 (lgr5), mRNA.

Price & Availability↑

CloneID	OXa07769
Clone ID Related Accession (Same CDS sequence)	XM_002940173.5 , XM_002940173.4
Accession Version	XM_002940173.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	leucine-rich repeat-containing G-protein coupled receptor 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940173.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCT CCAAGACCAG CTTCTTCTTG TTCTCGGTGC TGTGCAGCTT GCAATTGGTC 
GGCGCTGCTC GGCCTGGCAA ACAGCAGAGA AGCTGCCCGA CTCCATGTGA GTGCGAACAG 
GAGGGGATGT TGGTGCGAGT GGACTGCTCG GATCGGGCAC TGACTAGCCT GCCCAGGAAC 
CTCAGCATCT TCACGTCTTA CCTTGATCTA AGTATGAACA ACATTACCAA TCTGCCTTCC 
AATGTAATGC ACAACCTGCA CTTCTTAGAG GAGCTACGTC TTGCAGGAAA TGACTTGACA 
TACATCCCCA AGGGAGCTTT TGCTGGGCTC GGCAGCCTAA AAGTTTTAAT GCTACAGAAC 
AACCTGTTAA GGCAGGTACC GAGTGAAGCT CTTCACAACC TGCGCAGCCT TCAGTCTCTG 
CGCTTGGATG CAAACCATAT CAGCTATGTT CCACCAAGCT CTTTCAATGG TTTATTTTCC 
CTCCGTCACC TCTGGCTTGA TGACAATTCT TTGACTGAGA TTCCCGTGAG AGCACTTGAA 
AGCTTGTCTG CATTACAGGC TATGACCTTG GCACTAAACA AGATTCATCA TATACCAGAT 
TATGCCTTCA GAAACCTTTC AAGTCTGGTA GTTCTCCATC TCCATAACAA TAGAATCTAC 
TCCCTGGGAA AGAAATGCTT TGACGGGCTC CACAGTCTGG AGACTTTGGA CTTGAACTAC 
AATAATTTAG ATGAATTTCC TGCTGCCATA AAAACCCTAA AGAACCTTAA AGAATTGGGA 
TTTCATAGTA ACAACATCAA ATCGATCCCT GAGCAAGCAT TCATTGGCAA CCCTTCTCTA 
ATCACAATTC ACTTTTATGA CAACCCTATT CAACATGTGG GAAGATCAGC CTTTCAACAT 
TTGCCTGAGC TTAGAACTTT GATTCTTAAT GGGGCTTCAC AAATCACAGA ATTTCCTGAT 
CTAACTGGAA CTACAAGCCT GGAGAGTTTG ACTCTTACAG GAGCGCAGCT TGTGTACCTT 
CCCAGCGCTG TCTGCACTCA GCTGCCCAAC CTCCAAGTGC TGGACTTGTC ATATAATCAC 
ATCAAGGATT TGCCAAGCTT CTCCGGTTGT CAGCGACTGC AGAAAATAGA TCTGCGTCAT 
AACGAAGTCT ATGAAATTAG ATCCACCACC TTTCAGCAGC TGGTTGGCCT TCGTTCTCTA 
GACTTGGCAT GGAACAAGAT TGCTGTCATC CATCCTAACT CTTTTTCTTC TTTGCCTTCC 
TTAATCAAAT TGGATCTGTC ATCCAATCAT TTGACCTCAT TCCCTGTGAC CGGCCTTCAT 
GGTTTAACTC ATCTAAAGCT AACAGGAAAC TCAGCCCTTC AGGATCTGAT CCCATCTGAA 
CATTTCCCAA AGCTCAGGGT CATGGAGATG CCCTATGCCT ACCAGTGTTG TGCCTTTGCG 
GTTTGTGACA ATCTTAAGCA TTCAGGCCAG ATGAACAAAG ATGAAAACAG TAGTGCTGAT 
GATTTCTATC GCAAAGACAT AGGGCTGCTG CACCTTCAAG ATGACAGAGA CTTTGAAGAT 
TTTTTGCTTG ATTTTGAAGA AGATGTAAAA GTGCTGCATT CAGTACAGTG TACACCATCA 
GCAGGACCTT TCAAGCCTTG TGATCACCTT TTTGGAAGTT GGCTGACCAG AACTGGAGTG 
TGGCTTATAG TCCTCCTATC TTTTGTTTGT AATGCTTTGG TGATTGCAAC AGTCTTCCGA 
CCTCTGTCAT ATGTCCCTTC CATAAAGCTT CTCATTGGCC TAATAGCCAT TATGAATACA 
CTGATGGGCT TGTCCAGTGG TGTGCTAGCG ACTGTTGATG CCTTAACCTT TGGCAACTTT 
GCTCAGTATG GTGCCTGGTG GGAAAGTGGT GTAGGTTGCC AGATAACTGG GTTTCTGTCA 
GTTTTTGCAG CGGAGACCTC AATCTTTCTC CTTACTGTTG CTGCATTAGA GCGGGGGTTC 
TCCATTAAAT GTACTACAAA ATTTGAAACA AAGTCTTCAT TTATAAATGT CAAACTTTCC 
ATTGTCTTTT GCTTCCTTCT ATCCATTGTT ATTGCAGTTT CACCTTTGCT GAGTGGTAGT 
ACCTATGGGA CTTCTCCGTT GTGCTTTCCC CTGCTTTTTG GGGATCCCTC CTCTATGGGC 
TTTATGGTAG CTTTGGTTCT TCTAAACTCA TTATGCTTTC TTGTAATGAC TATTGCCTAT 
ACAAAACTCT ACTGCTCTCT AGAAAAAGGC GAATTGGAAA ATATATGGGA CTGCTCAATG 
GTGAAACATA TTGCCTTGCT GCTCTTCACA AACTGCATCC TATACTGCCC TGTAGCCTTT 
CTGTCTTTTT CCTCACTGTT AAATCTAACA TTCATCAGCC CTGAAGTGAA TAAGTCAATA 
CTCTTGTTGA TCATTCCACT CCCTGCATGT TTAAACCCTC TGCTATACAT CCTTTTTAAT 
CCACATTTCA AAGAAGATAT CGGAAGTCTG AAAAACGGAG ATATCCTGTG GAGTAGATCT 
AGACAAACGA GTTTGGCCTC AGTCAGTTCT GAAGATGCTG AAAAGCAGTC CTGTGATTCT 
ACACAAGCCC TTGTAACCTT TGCAAACTCC AGCATATCAT ATGATTTGCC AGCAACCAGC 
TCCTCATCAT CCTATCAGAT GACTAACAAC TATAAACTTT CAGCAGTGGC ATTTGTTCCT 
TGTCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940219.2
CDS	281..2989
Translation	MDTSKTSFFLFSVLCSLQLVGAARPGKQQRSCPTPCECEQEGMLVRVDCSDRALTSLPRNLSIFTSYLDLSMNNITNLPSNVMHNLHFLEELRLAGNDLTYIPKGAFAGLGSLKVLMLQNNLLRQVPSEALHNLRSLQSLRLDANHISYVPPSSFNGLFSLRHLWLDDNSLTEIPVRALESLSALQAMTLALNKIHHIPDYAFRNLSSLVVLHLHNNRIYSLGKKCFDGLHSLETLDLNYNNLDEFPAAIKTLKNLKELGFHSNNIKSIPEQAFIGNPSLITIHFYDNPIQHVGRSAFQHLPELRTLILNGASQITEFPDLTGTTSLESLTLTGAQLVYLPSAVCTQLPNLQVLDLSYNHIKDLPSFSGCQRLQKIDLRHNEVYEIRSTTFQQLVGLRSLDLAWNKIAVIHPNSFSSLPSLIKLDLSSNHLTSFPVTGLHGLTHLKLTGNSALQDLIPSEHFPKLRVMEMPYAYQCCAFAVCDNLKHSGQMNKDENSSADDFYRKDIGLLHLQDDRDFEDFLLDFEEDVKVLHSVQCTPSAGPFKPCDHLFGSWLTRTGVWLIVLLSFVCNALVIATVFRPLSYVPSIKLLIGLIAIMNTLMGLSSGVLATVDALTFGNFAQYGAWWESGVGCQITGFLSVFAAETSIFLLTVAALERGFSIKCTTKFETKSSFINVKLSIVFCFLLSIVIAVSPLLSGSTYGTSPLCFPLLFGDPSSMGFMVALVLLNSLCFLVMTIAYTKLYCSLEKGELENIWDCSMVKHIALLLFTNCILYCPVAFLSFSSLLNLTFISPEVNKSILLLIIPLPACLNPLLYILFNPHFKEDIGSLKNGDILWSRSRQTSLASVSSEDAEKQSCDSTQALVTFANSSISYDLPATSSSSSYQMTNNYKLSAVAFVPCH

Target ORF information:

RefSeq Version	XM_002940173.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis leucine-rich repeat containing G protein-coupled receptor 5 (lgr5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940173.5

ORF Insert Sequence:

ATGGACACCT CCAAGACCAG CTTCTTCTTG TTCTCGGTGC TGTGCAGCTT GCAATTGGTC 
GGCGCTGCTC GGCCTGGCAA ACAGCAGAGA AGCTGCCCGA CTCCATGTGA GTGCGAACAG 
GAGGGGATGT TGGTGCGAGT GGACTGCTCG GATCGGGCAC TGACTAGCCT GCCCAGGAAC 
CTCAGCATCT TCACGTCTTA CCTTGATCTA AGTATGAACA ACATTACCAA TCTGCCTTCC 
AATGTAATGC ACAACCTGCA CTTCTTAGAG GAGCTACGTC TTGCAGGAAA TGACTTGACA 
TACATCCCCA AGGGAGCTTT TGCTGGGCTC GGCAGCCTAA AAGTTTTAAT GCTACAGAAC 
AACCTGTTAA GGCAGGTACC GAGTGAAGCT CTTCACAACC TGCGCAGCCT TCAGTCTCTG 
CGCTTGGATG CAAACCATAT CAGCTATGTT CCACCAAGCT CTTTCAATGG TTTATTTTCC 
CTCCGTCACC TCTGGCTTGA TGACAATTCT TTGACTGAGA TTCCCGTGAG AGCACTTGAA 
AGCTTGTCTG CATTACAGGC TATGACCTTG GCACTAAACA AGATTCATCA TATACCAGAT 
TATGCCTTCA GAAACCTTTC AAGTCTGGTA GTTCTCCATC TCCATAACAA TAGAATCTAC 
TCCCTGGGAA AGAAATGCTT TGACGGGCTC CACAGTCTGG AGACTTTGGA CTTGAACTAC 
AATAATTTAG ATGAATTTCC TGCTGCCATA AAAACCCTAA AGAACCTTAA AGAATTGGGA 
TTTCATAGTA ACAACATCAA ATCGATCCCT GAGCAAGCAT TCATTGGCAA CCCTTCTCTA 
ATCACAATTC ACTTTTATGA CAACCCTATT CAACATGTGG GAAGATCAGC CTTTCAACAT 
TTGCCTGAGC TTAGAACTTT GATTCTTAAT GGGGCTTCAC AAATCACAGA ATTTCCTGAT 
CTAACTGGAA CTACAAGCCT GGAGAGTTTG ACTCTTACAG GAGCGCAGCT TGTGTACCTT 
CCCAGCGCTG TCTGCACTCA GCTGCCCAAC CTCCAAGTGC TGGACTTGTC ATATAATCAC 
ATCAAGGATT TGCCAAGCTT CTCCGGTTGT CAGCGACTGC AGAAAATAGA TCTGCGTCAT 
AACGAAGTCT ATGAAATTAG ATCCACCACC TTTCAGCAGC TGGTTGGCCT TCGTTCTCTA 
GACTTGGCAT GGAACAAGAT TGCTGTCATC CATCCTAACT CTTTTTCTTC TTTGCCTTCC 
TTAATCAAAT TGGATCTGTC ATCCAATCAT TTGACCTCAT TCCCTGTGAC CGGCCTTCAT 
GGTTTAACTC ATCTAAAGCT AACAGGAAAC TCAGCCCTTC AGGATCTGAT CCCATCTGAA 
CATTTCCCAA AGCTCAGGGT CATGGAGATG CCCTATGCCT ACCAGTGTTG TGCCTTTGCG 
GTTTGTGACA ATCTTAAGCA TTCAGGCCAG ATGAACAAAG ATGAAAACAG TAGTGCTGAT 
GATTTCTATC GCAAAGACAT AGGGCTGCTG CACCTTCAAG ATGACAGAGA CTTTGAAGAT 
TTTTTGCTTG ATTTTGAAGA AGATGTAAAA GTGCTGCATT CAGTACAGTG TACACCATCA 
GCAGGACCTT TCAAGCCTTG TGATCACCTT TTTGGAAGTT GGCTGACCAG AACTGGAGTG 
TGGCTTATAG TCCTCCTATC TTTTGTTTGT AATGCTTTGG TGATTGCAAC AGTCTTCCGA 
CCTCTGTCAT ATGTCCCTTC CATAAAGCTT CTCATTGGCC TAATAGCCAT TATGAATACA 
CTGATGGGCT TGTCCAGTGG TGTGCTAGCG ACTGTTGATG CCTTAACCTT TGGCAACTTT 
GCTCAGTATG GTGCCTGGTG GGAAAGTGGT GTAGGTTGCC AGATAACTGG GTTTCTGTCA 
GTTTTTGCAG CGGAGACCTC AATCTTTCTC CTTACTGTTG CTGCATTAGA GCGGGGGTTC 
TCCATTAAAT GTACTACAAA ATTTGAAACA AAGTCTTCAT TTATAAATGT CAAACTTTCC 
ATTGTCTTTT GCTTCCTTCT ATCCATTGTT ATTGCAGTTT CACCTTTGCT GAGTGGTAGT 
ACCTATGGGA CTTCTCCGTT GTGCTTTCCC CTGCTTTTTG GGGATCCCTC CTCTATGGGC 
TTTATGGTAG CTTTGGTTCT TCTAAACTCA TTATGCTTTC TTGTAATGAC TATTGCCTAT 
ACAAAACTCT ACTGCTCTCT AGAAAAAGGC GAATTGGAAA ATATATGGGA CTGCTCAATG 
GTGAAACATA TTGCCTTGCT GCTCTTCACA AACTGCATCC TATACTGCCC TGTAGCCTTT 
CTGTCTTTTT CCTCACTGTT AAATCTAACA TTCATCAGCC CTGAAGTGAA TAAGTCAATA 
CTCTTGTTGA TCATTCCACT CCCTGCATGT TTAAACCCTC TGCTATACAT CCTTTTTAAT 
CCACATTTCA AAGAAGATAT CGGAAGTCTG AAAAACGGAG ATATCCTGTG GAGTAGATCT 
AGACAAACGA GTTTGGCCTC AGTCAGTTCT GAAGATGCTG AAAAGCAGTC CTGTGATTCT 
ACACAAGCCC TTGTAACCTT TGCAAACTCC AGCATATCAT ATGATTTGCC AGCAACCAGC 
TCCTCATCAT CCTATCAGAT GACTAACAAC TATAAACTTT CAGCAGTGGC ATTTGTTCCT 
TGTCACTAA