sptbn2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following sptbn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sptbn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa56729 XM_002940505.5
Latest version!
Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa56730 XM_004913695.4
Latest version!
Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa56731 XM_012962405.3
Latest version!
Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OXa09015 XM_002940505.4
Latest version!
Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa09016 XM_004913695.3
Latest version!
Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OXa33551 XM_012962405.2
Latest version!
Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa56729
    Clone ID Related Accession (Same CDS sequence) XM_002940505.5
    Accession Version XM_002940505.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin beta chain, non-erythrocytic 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940505.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTTTCAGT TCGACTCCCC TGCATACGAG GATGTGCCCC GGCCTGCCTC CCCCGGTCCC 
    CGGCTTGCCT CCCCTGTCCC CCGATCTGGC TCCCCTCTCC TATCCCCTGC GGCTTTTGTA
    AATGCAGTGC AATACGCTAA CGTGCTGGAA GGAAGGTTCA AGCAGCTGCA AGATGAAAGA
    GAAGCTGTGC AGAAGAAGAC ATTTACTAAA TGGGTCAACT CTCACTTGGC GAGGGTCACC
    TGTAGAATTT CTGATCTCTA CAATGATTTA CGAGATGGAC GGATGTTAAT TCGACTGTTA
    GAAGTGCTAT CTGGAGAGCA GCTGCCAAAA CCCACAAAAG GTCGAATGCG AATCCACTGT
    TTGGAAAATG TAGACAAAGC CCTTCAATTT TTAAAAGAGC AGAAGGTACA TTTGGAGAAC
    ATGGGATCAC ATGACATTGT GGATGGAAAC CATCGATTAA CCCTAGGGTT AATCTGGACT
    ATTATTTTAA GGTTTCAGAT CCAAGATATC AGTGTAGAGA CAGAAGATAA TAAAGAAAAG
    AAGTCTGCTA AAGATGCCTT ACTGCTTTGG TGCCAGATGA AGACTGCTGG GTATCCAAAT
    GTCAATGTTC ACAACTTTAC CACCAGCTGG CGAGATGGAC TTGCGTTTAA TGCCATTGTA
    CACAAGCACA GACCAGATAT AATTGACTTT GACACACTGA AGAAGTCTAA TGCACACCAC
    AACCTCCAGA ATGCCTTCAA TGTAGCTGAA AGAGAGTTAG GTCTTACTAA ACTTCTGGAT
    CCTGAAGATG TTAATGTGGA TCAGCCTGAT GAGAAATCCA TCATAACCTA TGTGGCCACA
    TTTTATCACT ATTTTTCCAA AATGAAAGCC CTAGCTGTGG AAGGAAAGCG TATTGGAAAG
    GTACTTGACC AAGCTATTGA AGCTGATCAG TTAATTGAGA ACTATGAAGG ACTGGCATCA
    GAACTCCTGC AATGGATTGA ACAGATGATC ATTACTCTCA ATGACCGAAA ACTGGCAAAC
    TCTCTAAGTG GTGTTCAGAA CCAGCTGCAG GCCTTCAACA CCTACAGGAC TGTAGAAAAG
    CCGCCCAAGT TCACAGAAAA GGGCAATTTG GAAGTTCTTC TCTTTACCAT ACAGAGTAAA
    ATGAGAGCTA ATAATCAAAA AGTGTATACG CCCAGAGAAG GAAAGCTTAT ATCTGATATA
    AACAAGGCAT GGGAGCGACT AGAAAAGGCA GAGCATGAAC GTGAGCTTGC CCTTCGCAAT
    GAACTTATAC GCCAGGAAAA ATTAGAGCAG TTGGCTGCAC GATTTGACCG TAAAGCAGCC
    ATGAGGGAAA CCTGGCTAAG TGAAAATCAG AGACTTGTAG CTCAGGATAA CTTCGGTGTG
    GACTTAACTG CGGTGGAGGC AGCGCTACGG AAACATGAAG CTATTGAAAC TGATATATTG
    GCTTACAGTG AGCGCGTGCA AGCAGTGAAT GCAGTGGCTG ATGAGCTGGA AGCAGAGAAT
    TACCATGATG TCCGGAGGAT TTTGGCTCGG AAGTGCAATG TGATTCGTCT CTCTGATTAT
    TTAAGAGAGC TTGTCACTGC CAGGAGGCAA AGGCTTCATA TGAACCTAGA ATTGCAGAGG
    ATGTTCCAGG ATTTACATTA TCTCATGGAC TGGACTGAAG ATATGAAGGG CCGTCTGCAG
    TCAGAAGATT ATGGCAAGCA TCTTCATGGC GTGGAAGACC TTCTCCAGAC TCACACTCTT
    GTGGAGGCAG ACATTGCAGT TCAGGCAGAA CGTGTGAAAT CAGTTACAGC TGTAGCTCAG
    AAATTTATGA CAGAAGATGA AGGCTACAAG CCTTGCGACC CAGAGCTAGT GCGCAACCGG
    GTGCATCTCT TGGAACAGCG CTATGCAGAG CTGTGTAATC TTTCTGCGGC TCGGAGATCA
    AAGCTGGAAG AATCCCGCCG TCTCTGGAAA TTTTTCTGGG ACATGGGAGA GGAGGAAGCC
    TGGATCCGTG AGCAGAGTAG ATTACTACTT TCTGATGACT ATGGGAAAGA CCTTACCAGC
    TCACTTAGGT TGATCAGCAA GCATAATGCC TTCAGAGGTG AGATGAGTGG ACACCGTGGG
    CCGTTGCAGC AGACTGTGGC TGAGGGTGAG GAACTAATAT CCAAAGGTCA CTTTGGTGCA
    GGAGAGATTG AGGAAAGGAT AAAAGAAGTG AAAGGCCAGT GGACAGAGAT GGAGGCCTTA
    GCTGAAGACA GGGAAAGGAG GCTAAGAGAG GCCTACAGCT ATTATCAGTT TCAAGCTGAT
    GCCAATGATG CTGAGGCTTG GATCCTGGAC ACTTTACGCC TTGTTTCTAC CAGTGAAGTA
    GGACATGATG AGTATTCCAC ACAGACACTG GTGAAAAAGC ACAAAGAAAT AGAAGAGGAG
    ATCCTTAGCC ACCGACCAGC TATTGATGCG CTGCATGAAC AGGCCCGTGG CCTTCCACCT
    GCTTACTCTC ATTCACCTGA AGTTGATGGA AGGTTACCAG CCCTGGAGCA GCGCTATGAG
    GAATTGGTGG CTCTTGCTGC TCACCGCAAG CAGGCTCTGC AAGATTCTCT GGCTCTTCAT
    AAAATGTTTA GTGAAGCTGA TGCTTGCCTT TTGTGGATTG ATGAGAAAGA ACAGTGGCTT
    AATGTCACAG AGATTCCAGA AAAACTTGAG GACCTAGAAG TAGTTCAACA AAGGTTTGAA
    ACTCTAGAGG CAGAGATGAA TAATTTGGCA TCCCGGATTG CATCTGTAAA TGAAGTTGCT
    GGACAGTTGC TGACTACAGA CCAGAGAAAT GAAAACAGCA TCCGAGACAT GCAAGATCAA
    CTTAATTCTC GGTGGAATGA ATTCCGCAAG CTTGTTGACC AAAAGAAGGG TGCTTTGGAT
    TCTGCTCTGG ATATACAGAA TTATCATTTG GAGTGCAATG AGACTGTGTG CTGGATGAGG
    GAGAAGACAA AGGTTATTGA ATCCACACAG AGCCTAGGGA ATGATCTTGC AGGAGTAACA
    GCCCTCCAGA GAAAACTTAC TGGAATGGAG CGTGATCTGA AAGCCATTCA GGGAAAAGTG
    GGTGACCTGC GTTCAGAGGC AGAAAAGCTT GCAGATGGGC ACCCACAGAA AGCACCGGCC
    ATAATTGGCC GCTTATCAGA TATTGATGAG ACCTGGGGAG AACTAAAGGA CACTATGAAA
    AGAAGAGAAG AAACTTTAGG TGAAGCAAGC AAGCTGCAGG GATTTTTGAG AGACCTTGAT
    GATTTCCAGA GCTGGTTGTC CCGGGCCCAG ACAGCAGTGG CATCTGAGGA TGTTCCAGCT
    GACCTAGCAG AAGCAGAGCG CCTTCTTGCA CAACATGAAG GCATAAAAAA TGAGATTGAT
    CATTATGGAG CTGACTACCA CAGAGTGAGA GATGTTGGTG AAGAAGTCAC TCGTGGGCAA
    ACAGATGCCC AATATATGTT CTTGCAACAG AGACTGAAGG CGCTAGACAC TGGCTGGAAT
    GAGCTTGGAC AGATGTGGGA AAACAGGCAC CAGTTGTTGT CCCAGGGTCA CAGCTTCCAG
    CAGTTTTTGA AAGATACAAA ACAAGTGGAG GGGTTCCTTA ATAACCAGGA GTATGTCCTT
    TCCCATACCG AAATGCCAAG TACGCTACAG GCAGCTGAAG CTGCCATCAA ACGCCATGAA
    GATTTCCTCA CAACCATGGA TGCCAATGAA GAGAAGATAC AGGGTCTTGT AGAATCTGGG
    AGGAAGCTAA TTGAGGAACA GAATGTCAAT TCTGAGAAGA TTCAAGAAAA AGTCACTTCT
    ATTGATGAGA GACACAAAAA GAACCAAAAA ACTGCTAGCG AAGTATTAAC TCGTTTGAAG
    GACAACCGTG ATCTTCAACA TTTCCTTCAA GACTGTCAAG AGCTCACCCT CTGGATCAAT
    GAAAAAATGC TTACTGCTCA AGATGTTTCT TATGATGAAG CTCGAAACCT GCATAGCAAG
    TGGCAAAAGC ATCAGGCATT CATGGCAGAA CTGGCCTCAA ATAAAGACTG GCTTGACAAA
    ATAGACAAGG AAGGGCAGCA GCTCATGTCA GAAAAACCGG AGCTTGAACC AGTAGTGAGA
    GAAAAACTAG ACACTCTGCA TAAGCTGTGG AATGACTTGG AATCTACCAC ACAAAGTAAA
    GCTCAGTGTC TGTTTGATGC CAACAGGGCA GAGTTATTCA CACAAAGCTG TTCTGCTCTG
    GAGAGTTGGC TTCAAAACCT CGAGTCACAA CTACAGTCAG ATCATTATGG CAAAGATCTG
    ACCAGTGTCA ACATCCTATT AAATAAACAG CAGATGCTGG AGAACCAAAT GGAAGTGAGG
    GAGAAGGAGC TAGAAGCTAT ACAGTCCCAG GCACAGGCCC TTGGACAGGA GGATGCCAAC
    ATAGTGGAGG TTGACTCAAA GCATAAGGAT GTAGCAGATA AATTTTCCAA GCTGCGTGCT
    CCTCTAAACA AGAGAAGAAA ATTACTTCTA GCCTCCAAGG AAGGACATCA GTTTAACAGG
    GACCTGGAAG ATGAAATACT TTGGGTAAAG GAGAAGATGC CAATGGCAAT CTCCACTGAT
    CATGGAAAAG ATCTCCCGAC AGTCCAGCTT CTTATCAAAA AGAATCAGAC ACTCCAAAAG
    GAAATTTTGG GACATCAACC ACGGATTGAT GACATCCTGG AGAGAGGCAA ATCTATGATC
    CAAGACAGCT CTCTGGACCC TCAAGAGATA GAAGAAAGGC TTGGGCTACT GAAAAGCACA
    TGGGAAGAGC TGGCAGCAGA AGGAGGAAAG CGCCATGCCC GTTTAGAGGA ATCTCTTGCT
    GCTCAGCAGT TTTATGCTGA TGCGGCAGAA GCTGAGGCCT GGATGGGAGA GCAAGAGTTA
    CATATGATGT CTGAAGAAAA AGCCAAGGAT GAGATTGGTG CCCAGGCAAT GCTGAAGAAA
    CACTTAGTAA TGGAACAGGC TCTTGATGAC TATGCAGACA ACATCCATCA GCTTGCCAAT
    TCAAGCCGCG ACATGGTCTC CAGAGGTCAT CCAGAAAGTG ATCGTATTAC CCTTCGTCAA
    GGGCAAGTGG ACAAGCTATA TGCCAGTCTT AAAGACCTGG CAGAGGAGAG ACAAGGAAAG
    CTACAAGAGC ACCACAGACT TTGCCAACTC AAGAGAGATG TCGAAGACCT TGAGCACTGG
    ATCTCTGAAC GTGAGGTGGT TGCAGCTTCT CATGAACTTG GGCAAGATTA TGAGCATGTA
    ACGATGCTGA GAGACAAATT CCGTGAGTTC TCCCGTGACA CCAGTAACAT TGGTCAAGAG
    CGGGTGGATG CTGTCAATCT GATGGCAGAT GATTTAATTT CATCTGGACA TTCGGAAAAT
    GCTACTGTAG CAGATTGGAA AGACCAGCTA AATGAGGCAT GGGCTGATCT TCTTGAGATG
    ATTGACACTC GAAGTCAGAT GCTGGCAGCC TCATATGAAC TTCACCGTTT CTACCAGGAT
    GCAAAAGAAA CCCTAGGTCA GATCCAAGAC AAGCAGAAAC AAATACCAGA GGAGACAGGC
    AGAGATCTGA ATACAGCAGA AGCCATGCAG CGCATGCACA CCGCTTTTGA ACATGATATC
    CAAGCCCTGA GTGCACAGGT GAAGCAAGTC CAGGAAGATG CTTCTCGCCT CCAAAAAGCT
    TATGCTGGTG AAAAGGCAGC AGACATTCAT CGCCATGAGC AGGCAGTATC GGAAGCTTGG
    GGAGAGTTGC AGAATGCTAG TGGTGCCAGG CGGCAGCTTC TTCTTGACAC TGTGGAGAAA
    TTCCGTTTCT TCAGAATGGT GCGAGATCTC ATGTTATGGA TGGATGATAT AAACCTGCTA
    ATTGATGCAC AGGAGAAGCC AAGAGATGTA TCCTCAGCTG ATTTAGTCAT CCAAAACCAC
    CTAGGCATCA AGGCAGAAGT GGATGCGCGA GCTGACAGCT TTACTACATG CATAGATCTT
    GGCAACTCTC TCCTAGAGAA GAATCATTAT GCCTCTGACA AGATTTCAGA GAAACTGGAG
    CAATTGCAGG AAAGAAGGCA GGAGACTGAA AACAAATGGA AAGATAAAAT GGCATGGCTG
    AGAGTTGTCC ACGAAGTGCT GATGTTTGGA AGAGATGCAA GTGTAGCTGA GGCTTGGCTG
    GCCAGCCAGG AGCCCATTGT CCGGTCGTCA GAACTTGGTG GCAATGTCGA TGAAGTAGAA
    TCCCTGATAA AAAGACACGA AGCCTTTCAG AAAGCAGTTT CTACATGGGA TGAGAGATTT
    GTTCTTCTTG AAAAACTAAC CACGTTAGAA GAAATTGAAA TGCGTCGGAA GCGGGAAGAG
    GAAGAAAGAA AGAAAAGACC ACCAACACCA CCTCTACCAG TAGAAGAGCC TCGAGAGGAA
    GTTGTAAAAA CCCAAGTTTC CCAGACTATT GAAGGGCAGA CTGATCCAGC CAATATACTT
    CAAGAAAGGA TAAATGGCAT GCAGAATTCA TTCCAGGAGC AAGTATCTGT TAATGGACTG
    TGCACAGAGT CTGATTCTCC ACAGCCATCG TCCGATACAC AGAGGTTAGA AGACAGCATC
    TCTGAAAATC AGGGTCCGGA ACAGTCAGAT GTAACCAATG GAGCCAGGAC TGAGGTTATT
    ACCAAAGATG GAAGTCCAAC ACCTTCTCCA CAACTTGTGG CTAGGCCAAC CTTCTCCCCG
    ATAAACCTCC AGTATTCTAC TTTGCCATCT AGCCCCACTG AACCATTGGC ATCTGAGCAA
    ATGGAAGGAA TGCTGCACCG CAAGCAGGAA ATGGATTCTC ACAACAAGAA GGCTGCGAAC
    AGGTCCTGGC AGAATGTTTA CTGTGCACTG AAAAGGGGCA GTCTGGGTTT CTACAAGGAT
    GCTAAAAGTG CAGCCAGTGG CGTTTCTTAT CATTCGGAGC CCTCCGTCCA ACTTAATGGA
    GCCCAGTGCA GTGTTGCTCA TGACTACAAG AAACGCAAGA ATGTCTTCAA GCTAAGCCTT
    CTTGATGGCA AAGAATATCT GTTCCAAGCT AAGGATGAGG CTGAAATGAG TAGTTGGATG
    CGAGTTATAA ACGCCTCCCT AACTTCCTCC CCGGGAGACC CCTCAGACCA TTCACCGGGC
    ATCATGAAGG GTATGACCCG GGCCATGACA ATGCCACCAG TATCACCCAA CAGTGGTACA
    GAGGGATCAG TCACATTGCG CAACAAGGAC GGGAAAGAAA AAGACCGGGA AAAGAGATTC
    AGTTTCTTTA AGAAGAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002940551.2
    CDS909..8129
    Translation

    Target ORF information:

    RefSeq Version XM_002940505.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002940505.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTTTCAGT TCGACTCCCC TGCATACGAG GATGTGCCCC GGCCTGCCTC CCCCGGTCCC 
    CGGCTTGCCT CCCCTGTCCC CCGATCTGGC TCCCCTCTCC TATCCCCTGC GGCTTTTGTA
    AATGCAGTGC AATACGCTAA CGTGCTGGAA GGAAGGTTCA AGCAGCTGCA AGATGAAAGA
    GAAGCTGTGC AGAAGAAGAC ATTTACTAAA TGGGTCAACT CTCACTTGGC GAGGGTCACC
    TGTAGAATTT CTGATCTCTA CAATGATTTA CGAGATGGAC GGATGTTAAT TCGACTGTTA
    GAAGTGCTAT CTGGAGAGCA GCTGCCAAAA CCCACAAAAG GTCGAATGCG AATCCACTGT
    TTGGAAAATG TAGACAAAGC CCTTCAATTT TTAAAAGAGC AGAAGGTACA TTTGGAGAAC
    ATGGGATCAC ATGACATTGT GGATGGAAAC CATCGATTAA CCCTAGGGTT AATCTGGACT
    ATTATTTTAA GGTTTCAGAT CCAAGATATC AGTGTAGAGA CAGAAGATAA TAAAGAAAAG
    AAGTCTGCTA AAGATGCCTT ACTGCTTTGG TGCCAGATGA AGACTGCTGG GTATCCAAAT
    GTCAATGTTC ACAACTTTAC CACCAGCTGG CGAGATGGAC TTGCGTTTAA TGCCATTGTA
    CACAAGCACA GACCAGATAT AATTGACTTT GACACACTGA AGAAGTCTAA TGCACACCAC
    AACCTCCAGA ATGCCTTCAA TGTAGCTGAA AGAGAGTTAG GTCTTACTAA ACTTCTGGAT
    CCTGAAGATG TTAATGTGGA TCAGCCTGAT GAGAAATCCA TCATAACCTA TGTGGCCACA
    TTTTATCACT ATTTTTCCAA AATGAAAGCC CTAGCTGTGG AAGGAAAGCG TATTGGAAAG
    GTACTTGACC AAGCTATTGA AGCTGATCAG TTAATTGAGA ACTATGAAGG ACTGGCATCA
    GAACTCCTGC AATGGATTGA ACAGATGATC ATTACTCTCA ATGACCGAAA ACTGGCAAAC
    TCTCTAAGTG GTGTTCAGAA CCAGCTGCAG GCCTTCAACA CCTACAGGAC TGTAGAAAAG
    CCGCCCAAGT TCACAGAAAA GGGCAATTTG GAAGTTCTTC TCTTTACCAT ACAGAGTAAA
    ATGAGAGCTA ATAATCAAAA AGTGTATACG CCCAGAGAAG GAAAGCTTAT ATCTGATATA
    AACAAGGCAT GGGAGCGACT AGAAAAGGCA GAGCATGAAC GTGAGCTTGC CCTTCGCAAT
    GAACTTATAC GCCAGGAAAA ATTAGAGCAG TTGGCTGCAC GATTTGACCG TAAAGCAGCC
    ATGAGGGAAA CCTGGCTAAG TGAAAATCAG AGACTTGTAG CTCAGGATAA CTTCGGTGTG
    GACTTAACTG CGGTGGAGGC AGCGCTACGG AAACATGAAG CTATTGAAAC TGATATATTG
    GCTTACAGTG AGCGCGTGCA AGCAGTGAAT GCAGTGGCTG ATGAGCTGGA AGCAGAGAAT
    TACCATGATG TCCGGAGGAT TTTGGCTCGG AAGTGCAATG TGATTCGTCT CTCTGATTAT
    TTAAGAGAGC TTGTCACTGC CAGGAGGCAA AGGCTTCATA TGAACCTAGA ATTGCAGAGG
    ATGTTCCAGG ATTTACATTA TCTCATGGAC TGGACTGAAG ATATGAAGGG CCGTCTGCAG
    TCAGAAGATT ATGGCAAGCA TCTTCATGGC GTGGAAGACC TTCTCCAGAC TCACACTCTT
    GTGGAGGCAG ACATTGCAGT TCAGGCAGAA CGTGTGAAAT CAGTTACAGC TGTAGCTCAG
    AAATTTATGA CAGAAGATGA AGGCTACAAG CCTTGCGACC CAGAGCTAGT GCGCAACCGG
    GTGCATCTCT TGGAACAGCG CTATGCAGAG CTGTGTAATC TTTCTGCGGC TCGGAGATCA
    AAGCTGGAAG AATCCCGCCG TCTCTGGAAA TTTTTCTGGG ACATGGGAGA GGAGGAAGCC
    TGGATCCGTG AGCAGAGTAG ATTACTACTT TCTGATGACT ATGGGAAAGA CCTTACCAGC
    TCACTTAGGT TGATCAGCAA GCATAATGCC TTCAGAGGTG AGATGAGTGG ACACCGTGGG
    CCGTTGCAGC AGACTGTGGC TGAGGGTGAG GAACTAATAT CCAAAGGTCA CTTTGGTGCA
    GGAGAGATTG AGGAAAGGAT AAAAGAAGTG AAAGGCCAGT GGACAGAGAT GGAGGCCTTA
    GCTGAAGACA GGGAAAGGAG GCTAAGAGAG GCCTACAGCT ATTATCAGTT TCAAGCTGAT
    GCCAATGATG CTGAGGCTTG GATCCTGGAC ACTTTACGCC TTGTTTCTAC CAGTGAAGTA
    GGACATGATG AGTATTCCAC ACAGACACTG GTGAAAAAGC ACAAAGAAAT AGAAGAGGAG
    ATCCTTAGCC ACCGACCAGC TATTGATGCG CTGCATGAAC AGGCCCGTGG CCTTCCACCT
    GCTTACTCTC ATTCACCTGA AGTTGATGGA AGGTTACCAG CCCTGGAGCA GCGCTATGAG
    GAATTGGTGG CTCTTGCTGC TCACCGCAAG CAGGCTCTGC AAGATTCTCT GGCTCTTCAT
    AAAATGTTTA GTGAAGCTGA TGCTTGCCTT TTGTGGATTG ATGAGAAAGA ACAGTGGCTT
    AATGTCACAG AGATTCCAGA AAAACTTGAG GACCTAGAAG TAGTTCAACA AAGGTTTGAA
    ACTCTAGAGG CAGAGATGAA TAATTTGGCA TCCCGGATTG CATCTGTAAA TGAAGTTGCT
    GGACAGTTGC TGACTACAGA CCAGAGAAAT GAAAACAGCA TCCGAGACAT GCAAGATCAA
    CTTAATTCTC GGTGGAATGA ATTCCGCAAG CTTGTTGACC AAAAGAAGGG TGCTTTGGAT
    TCTGCTCTGG ATATACAGAA TTATCATTTG GAGTGCAATG AGACTGTGTG CTGGATGAGG
    GAGAAGACAA AGGTTATTGA ATCCACACAG AGCCTAGGGA ATGATCTTGC AGGAGTAACA
    GCCCTCCAGA GAAAACTTAC TGGAATGGAG CGTGATCTGA AAGCCATTCA GGGAAAAGTG
    GGTGACCTGC GTTCAGAGGC AGAAAAGCTT GCAGATGGGC ACCCACAGAA AGCACCGGCC
    ATAATTGGCC GCTTATCAGA TATTGATGAG ACCTGGGGAG AACTAAAGGA CACTATGAAA
    AGAAGAGAAG AAACTTTAGG TGAAGCAAGC AAGCTGCAGG GATTTTTGAG AGACCTTGAT
    GATTTCCAGA GCTGGTTGTC CCGGGCCCAG ACAGCAGTGG CATCTGAGGA TGTTCCAGCT
    GACCTAGCAG AAGCAGAGCG CCTTCTTGCA CAACATGAAG GCATAAAAAA TGAGATTGAT
    CATTATGGAG CTGACTACCA CAGAGTGAGA GATGTTGGTG AAGAAGTCAC TCGTGGGCAA
    ACAGATGCCC AATATATGTT CTTGCAACAG AGACTGAAGG CGCTAGACAC TGGCTGGAAT
    GAGCTTGGAC AGATGTGGGA AAACAGGCAC CAGTTGTTGT CCCAGGGTCA CAGCTTCCAG
    CAGTTTTTGA AAGATACAAA ACAAGTGGAG GGGTTCCTTA ATAACCAGGA GTATGTCCTT
    TCCCATACCG AAATGCCAAG TACGCTACAG GCAGCTGAAG CTGCCATCAA ACGCCATGAA
    GATTTCCTCA CAACCATGGA TGCCAATGAA GAGAAGATAC AGGGTCTTGT AGAATCTGGG
    AGGAAGCTAA TTGAGGAACA GAATGTCAAT TCTGAGAAGA TTCAAGAAAA AGTCACTTCT
    ATTGATGAGA GACACAAAAA GAACCAAAAA ACTGCTAGCG AAGTATTAAC TCGTTTGAAG
    GACAACCGTG ATCTTCAACA TTTCCTTCAA GACTGTCAAG AGCTCACCCT CTGGATCAAT
    GAAAAAATGC TTACTGCTCA AGATGTTTCT TATGATGAAG CTCGAAACCT GCATAGCAAG
    TGGCAAAAGC ATCAGGCATT CATGGCAGAA CTGGCCTCAA ATAAAGACTG GCTTGACAAA
    ATAGACAAGG AAGGGCAGCA GCTCATGTCA GAAAAACCGG AGCTTGAACC AGTAGTGAGA
    GAAAAACTAG ACACTCTGCA TAAGCTGTGG AATGACTTGG AATCTACCAC ACAAAGTAAA
    GCTCAGTGTC TGTTTGATGC CAACAGGGCA GAGTTATTCA CACAAAGCTG TTCTGCTCTG
    GAGAGTTGGC TTCAAAACCT CGAGTCACAA CTACAGTCAG ATCATTATGG CAAAGATCTG
    ACCAGTGTCA ACATCCTATT AAATAAACAG CAGATGCTGG AGAACCAAAT GGAAGTGAGG
    GAGAAGGAGC TAGAAGCTAT ACAGTCCCAG GCACAGGCCC TTGGACAGGA GGATGCCAAC
    ATAGTGGAGG TTGACTCAAA GCATAAGGAT GTAGCAGATA AATTTTCCAA GCTGCGTGCT
    CCTCTAAACA AGAGAAGAAA ATTACTTCTA GCCTCCAAGG AAGGACATCA GTTTAACAGG
    GACCTGGAAG ATGAAATACT TTGGGTAAAG GAGAAGATGC CAATGGCAAT CTCCACTGAT
    CATGGAAAAG ATCTCCCGAC AGTCCAGCTT CTTATCAAAA AGAATCAGAC ACTCCAAAAG
    GAAATTTTGG GACATCAACC ACGGATTGAT GACATCCTGG AGAGAGGCAA ATCTATGATC
    CAAGACAGCT CTCTGGACCC TCAAGAGATA GAAGAAAGGC TTGGGCTACT GAAAAGCACA
    TGGGAAGAGC TGGCAGCAGA AGGAGGAAAG CGCCATGCCC GTTTAGAGGA ATCTCTTGCT
    GCTCAGCAGT TTTATGCTGA TGCGGCAGAA GCTGAGGCCT GGATGGGAGA GCAAGAGTTA
    CATATGATGT CTGAAGAAAA AGCCAAGGAT GAGATTGGTG CCCAGGCAAT GCTGAAGAAA
    CACTTAGTAA TGGAACAGGC TCTTGATGAC TATGCAGACA ACATCCATCA GCTTGCCAAT
    TCAAGCCGCG ACATGGTCTC CAGAGGTCAT CCAGAAAGTG ATCGTATTAC CCTTCGTCAA
    GGGCAAGTGG ACAAGCTATA TGCCAGTCTT AAAGACCTGG CAGAGGAGAG ACAAGGAAAG
    CTACAAGAGC ACCACAGACT TTGCCAACTC AAGAGAGATG TCGAAGACCT TGAGCACTGG
    ATCTCTGAAC GTGAGGTGGT TGCAGCTTCT CATGAACTTG GGCAAGATTA TGAGCATGTA
    ACGATGCTGA GAGACAAATT CCGTGAGTTC TCCCGTGACA CCAGTAACAT TGGTCAAGAG
    CGGGTGGATG CTGTCAATCT GATGGCAGAT GATTTAATTT CATCTGGACA TTCGGAAAAT
    GCTACTGTAG CAGATTGGAA AGACCAGCTA AATGAGGCAT GGGCTGATCT TCTTGAGATG
    ATTGACACTC GAAGTCAGAT GCTGGCAGCC TCATATGAAC TTCACCGTTT CTACCAGGAT
    GCAAAAGAAA CCCTAGGTCA GATCCAAGAC AAGCAGAAAC AAATACCAGA GGAGACAGGC
    AGAGATCTGA ATACAGCAGA AGCCATGCAG CGCATGCACA CCGCTTTTGA ACATGATATC
    CAAGCCCTGA GTGCACAGGT GAAGCAAGTC CAGGAAGATG CTTCTCGCCT CCAAAAAGCT
    TATGCTGGTG AAAAGGCAGC AGACATTCAT CGCCATGAGC AGGCAGTATC GGAAGCTTGG
    GGAGAGTTGC AGAATGCTAG TGGTGCCAGG CGGCAGCTTC TTCTTGACAC TGTGGAGAAA
    TTCCGTTTCT TCAGAATGGT GCGAGATCTC ATGTTATGGA TGGATGATAT AAACCTGCTA
    ATTGATGCAC AGGAGAAGCC AAGAGATGTA TCCTCAGCTG ATTTAGTCAT CCAAAACCAC
    CTAGGCATCA AGGCAGAAGT GGATGCGCGA GCTGACAGCT TTACTACATG CATAGATCTT
    GGCAACTCTC TCCTAGAGAA GAATCATTAT GCCTCTGACA AGATTTCAGA GAAACTGGAG
    CAATTGCAGG AAAGAAGGCA GGAGACTGAA AACAAATGGA AAGATAAAAT GGCATGGCTG
    AGAGTTGTCC ACGAAGTGCT GATGTTTGGA AGAGATGCAA GTGTAGCTGA GGCTTGGCTG
    GCCAGCCAGG AGCCCATTGT CCGGTCGTCA GAACTTGGTG GCAATGTCGA TGAAGTAGAA
    TCCCTGATAA AAAGACACGA AGCCTTTCAG AAAGCAGTTT CTACATGGGA TGAGAGATTT
    GTTCTTCTTG AAAAACTAAC CACGTTAGAA GAAATTGAAA TGCGTCGGAA GCGGGAAGAG
    GAAGAAAGAA AGAAAAGACC ACCAACACCA CCTCTACCAG TAGAAGAGCC TCGAGAGGAA
    GTTGTAAAAA CCCAAGTTTC CCAGACTATT GAAGGGCAGA CTGATCCAGC CAATATACTT
    CAAGAAAGGA TAAATGGCAT GCAGAATTCA TTCCAGGAGC AAGTATCTGT TAATGGACTG
    TGCACAGAGT CTGATTCTCC ACAGCCATCG TCCGATACAC AGAGGTTAGA AGACAGCATC
    TCTGAAAATC AGGGTCCGGA ACAGTCAGAT GTAACCAATG GAGCCAGGAC TGAGGTTATT
    ACCAAAGATG GAAGTCCAAC ACCTTCTCCA CAACTTGTGG CTAGGCCAAC CTTCTCCCCG
    ATAAACCTCC AGTATTCTAC TTTGCCATCT AGCCCCACTG AACCATTGGC ATCTGAGCAA
    ATGGAAGGAA TGCTGCACCG CAAGCAGGAA ATGGATTCTC ACAACAAGAA GGCTGCGAAC
    AGGTCCTGGC AGAATGTTTA CTGTGCACTG AAAAGGGGCA GTCTGGGTTT CTACAAGGAT
    GCTAAAAGTG CAGCCAGTGG CGTTTCTTAT CATTCGGAGC CCTCCGTCCA ACTTAATGGA
    GCCCAGTGCA GTGTTGCTCA TGACTACAAG AAACGCAAGA ATGTCTTCAA GCTAAGCCTT
    CTTGATGGCA AAGAATATCT GTTCCAAGCT AAGGATGAGG CTGAAATGAG TAGTTGGATG
    CGAGTTATAA ACGCCTCCCT AACTTCCTCC CCGGGAGACC CCTCAGACCA TTCACCGGGC
    ATCATGAAGG GTATGACCCG GGCCATGACA ATGCCACCAG TATCACCCAA CAGTGGTACA
    GAGGGATCAG TCACATTGCG CAACAAGGAC GGGAAAGAAA AAGACCGGGA AAAGAGATTC
    AGTTTCTTTA AGAAGAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56730
    Clone ID Related Accession (Same CDS sequence) XM_004913695.4
    Accession Version XM_004913695.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004913695.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
    GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA
    AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGATG AAAGAGAAGC TGTGCAGAAG
    AAGACATTTA CTAAATGGGT CAACTCTCAC TTGGCGAGGG TCACCTGTAG AATTTCTGAT
    CTCTACAATG ATTTACGAGA TGGACGGATG TTAATTCGAC TGTTAGAAGT GCTATCTGGA
    GAGCAGCTGC CAAAACCCAC AAAAGGTCGA ATGCGAATCC ACTGTTTGGA AAATGTAGAC
    AAAGCCCTTC AATTTTTAAA AGAGCAGAAG GTACATTTGG AGAACATGGG ATCACATGAC
    ATTGTGGATG GAAACCATCG ATTAACCCTA GGGTTAATCT GGACTATTAT TTTAAGGTTT
    CAGATCCAAG ATATCAGTGT AGAGACAGAA GATAATAAAG AAAAGAAGTC TGCTAAAGAT
    GCCTTACTGC TTTGGTGCCA GATGAAGACT GCTGGGTATC CAAATGTCAA TGTTCACAAC
    TTTACCACCA GCTGGCGAGA TGGACTTGCG TTTAATGCCA TTGTACACAA GCACAGACCA
    GATATAATTG ACTTTGACAC ACTGAAGAAG TCTAATGCAC ACCACAACCT CCAGAATGCC
    TTCAATGTAG CTGAAAGAGA GTTAGGTCTT ACTAAACTTC TGGATCCTGA AGATGTTAAT
    GTGGATCAGC CTGATGAGAA ATCCATCATA ACCTATGTGG CCACATTTTA TCACTATTTT
    TCCAAAATGA AAGCCCTAGC TGTGGAAGGA AAGCGTATTG GAAAGGTACT TGACCAAGCT
    ATTGAAGCTG ATCAGTTAAT TGAGAACTAT GAAGGACTGG CATCAGAACT CCTGCAATGG
    ATTGAACAGA TGATCATTAC TCTCAATGAC CGAAAACTGG CAAACTCTCT AAGTGGTGTT
    CAGAACCAGC TGCAGGCCTT CAACACCTAC AGGACTGTAG AAAAGCCGCC CAAGTTCACA
    GAAAAGGGCA ATTTGGAAGT TCTTCTCTTT ACCATACAGA GTAAAATGAG AGCTAATAAT
    CAAAAAGTGT ATACGCCCAG AGAAGGAAAG CTTATATCTG ATATAAACAA GGCATGGGAG
    CGACTAGAAA AGGCAGAGCA TGAACGTGAG CTTGCCCTTC GCAATGAACT TATACGCCAG
    GAAAAATTAG AGCAGTTGGC TGCACGATTT GACCGTAAAG CAGCCATGAG GGAAACCTGG
    CTAAGTGAAA ATCAGAGACT TGTAGCTCAG GATAACTTCG GTGTGGACTT AACTGCGGTG
    GAGGCAGCGC TACGGAAACA TGAAGCTATT GAAACTGATA TATTGGCTTA CAGTGAGCGC
    GTGCAAGCAG TGAATGCAGT GGCTGATGAG CTGGAAGCAG AGAATTACCA TGATGTCCGG
    AGGATTTTGG CTCGGAAGTG CAATGTGATT CGTCTCTCTG ATTATTTAAG AGAGCTTGTC
    ACTGCCAGGA GGCAAAGGCT TCATATGAAC CTAGAATTGC AGAGGATGTT CCAGGATTTA
    CATTATCTCA TGGACTGGAC TGAAGATATG AAGGGCCGTC TGCAGTCAGA AGATTATGGC
    AAGCATCTTC ATGGCGTGGA AGACCTTCTC CAGACTCACA CTCTTGTGGA GGCAGACATT
    GCAGTTCAGG CAGAACGTGT GAAATCAGTT ACAGCTGTAG CTCAGAAATT TATGACAGAA
    GATGAAGGCT ACAAGCCTTG CGACCCAGAG CTAGTGCGCA ACCGGGTGCA TCTCTTGGAA
    CAGCGCTATG CAGAGCTGTG TAATCTTTCT GCGGCTCGGA GATCAAAGCT GGAAGAATCC
    CGCCGTCTCT GGAAATTTTT CTGGGACATG GGAGAGGAGG AAGCCTGGAT CCGTGAGCAG
    AGTAGATTAC TACTTTCTGA TGACTATGGG AAAGACCTTA CCAGCTCACT TAGGTTGATC
    AGCAAGCATA ATGCCTTCAG AGGTGAGATG AGTGGACACC GTGGGCCGTT GCAGCAGACT
    GTGGCTGAGG GTGAGGAACT AATATCCAAA GGTCACTTTG GTGCAGGAGA GATTGAGGAA
    AGGATAAAAG AAGTGAAAGG CCAGTGGACA GAGATGGAGG CCTTAGCTGA AGACAGGGAA
    AGGAGGCTAA GAGAGGCCTA CAGCTATTAT CAGTTTCAAG CTGATGCCAA TGATGCTGAG
    GCTTGGATCC TGGACACTTT ACGCCTTGTT TCTACCAGTG AAGTAGGACA TGATGAGTAT
    TCCACACAGA CACTGGTGAA AAAGCACAAA GAAATAGAAG AGGAGATCCT TAGCCACCGA
    CCAGCTATTG ATGCGCTGCA TGAACAGGCC CGTGGCCTTC CACCTGCTTA CTCTCATTCA
    CCTGAAGTTG ATGGAAGGTT ACCAGCCCTG GAGCAGCGCT ATGAGGAATT GGTGGCTCTT
    GCTGCTCACC GCAAGCAGGC TCTGCAAGAT TCTCTGGCTC TTCATAAAAT GTTTAGTGAA
    GCTGATGCTT GCCTTTTGTG GATTGATGAG AAAGAACAGT GGCTTAATGT CACAGAGATT
    CCAGAAAAAC TTGAGGACCT AGAAGTAGTT CAACAAAGGT TTGAAACTCT AGAGGCAGAG
    ATGAATAATT TGGCATCCCG GATTGCATCT GTAAATGAAG TTGCTGGACA GTTGCTGACT
    ACAGACCAGA GAAATGAAAA CAGCATCCGA GACATGCAAG ATCAACTTAA TTCTCGGTGG
    AATGAATTCC GCAAGCTTGT TGACCAAAAG AAGGGTGCTT TGGATTCTGC TCTGGATATA
    CAGAATTATC ATTTGGAGTG CAATGAGACT GTGTGCTGGA TGAGGGAGAA GACAAAGGTT
    ATTGAATCCA CACAGAGCCT AGGGAATGAT CTTGCAGGAG TAACAGCCCT CCAGAGAAAA
    CTTACTGGAA TGGAGCGTGA TCTGAAAGCC ATTCAGGGAA AAGTGGGTGA CCTGCGTTCA
    GAGGCAGAAA AGCTTGCAGA TGGGCACCCA CAGAAAGCAC CGGCCATAAT TGGCCGCTTA
    TCAGATATTG ATGAGACCTG GGGAGAACTA AAGGACACTA TGAAAAGAAG AGAAGAAACT
    TTAGGTGAAG CAAGCAAGCT GCAGGGATTT TTGAGAGACC TTGATGATTT CCAGAGCTGG
    TTGTCCCGGG CCCAGACAGC AGTGGCATCT GAGGATGTTC CAGCTGACCT AGCAGAAGCA
    GAGCGCCTTC TTGCACAACA TGAAGGCATA AAAAATGAGA TTGATCATTA TGGAGCTGAC
    TACCACAGAG TGAGAGATGT TGGTGAAGAA GTCACTCGTG GGCAAACAGA TGCCCAATAT
    ATGTTCTTGC AACAGAGACT GAAGGCGCTA GACACTGGCT GGAATGAGCT TGGACAGATG
    TGGGAAAACA GGCACCAGTT GTTGTCCCAG GGTCACAGCT TCCAGCAGTT TTTGAAAGAT
    ACAAAACAAG TGGAGGGGTT CCTTAATAAC CAGGAGTATG TCCTTTCCCA TACCGAAATG
    CCAAGTACGC TACAGGCAGC TGAAGCTGCC ATCAAACGCC ATGAAGATTT CCTCACAACC
    ATGGATGCCA ATGAAGAGAA GATACAGGGT CTTGTAGAAT CTGGGAGGAA GCTAATTGAG
    GAACAGAATG TCAATTCTGA GAAGATTCAA GAAAAAGTCA CTTCTATTGA TGAGAGACAC
    AAAAAGAACC AAAAAACTGC TAGCGAAGTA TTAACTCGTT TGAAGGACAA CCGTGATCTT
    CAACATTTCC TTCAAGACTG TCAAGAGCTC ACCCTCTGGA TCAATGAAAA AATGCTTACT
    GCTCAAGATG TTTCTTATGA TGAAGCTCGA AACCTGCATA GCAAGTGGCA AAAGCATCAG
    GCATTCATGG CAGAACTGGC CTCAAATAAA GACTGGCTTG ACAAAATAGA CAAGGAAGGG
    CAGCAGCTCA TGTCAGAAAA ACCGGAGCTT GAACCAGTAG TGAGAGAAAA ACTAGACACT
    CTGCATAAGC TGTGGAATGA CTTGGAATCT ACCACACAAA GTAAAGCTCA GTGTCTGTTT
    GATGCCAACA GGGCAGAGTT ATTCACACAA AGCTGTTCTG CTCTGGAGAG TTGGCTTCAA
    AACCTCGAGT CACAACTACA GTCAGATCAT TATGGCAAAG ATCTGACCAG TGTCAACATC
    CTATTAAATA AACAGCAGAT GCTGGAGAAC CAAATGGAAG TGAGGGAGAA GGAGCTAGAA
    GCTATACAGT CCCAGGCACA GGCCCTTGGA CAGGAGGATG CCAACATAGT GGAGGTTGAC
    TCAAAGCATA AGGATGTAGC AGATAAATTT TCCAAGCTGC GTGCTCCTCT AAACAAGAGA
    AGAAAATTAC TTCTAGCCTC CAAGGAAGGA CATCAGTTTA ACAGGGACCT GGAAGATGAA
    ATACTTTGGG TAAAGGAGAA GATGCCAATG GCAATCTCCA CTGATCATGG AAAAGATCTC
    CCGACAGTCC AGCTTCTTAT CAAAAAGAAT CAGACACTCC AAAAGGAAAT TTTGGGACAT
    CAACCACGGA TTGATGACAT CCTGGAGAGA GGCAAATCTA TGATCCAAGA CAGCTCTCTG
    GACCCTCAAG AGATAGAAGA AAGGCTTGGG CTACTGAAAA GCACATGGGA AGAGCTGGCA
    GCAGAAGGAG GAAAGCGCCA TGCCCGTTTA GAGGAATCTC TTGCTGCTCA GCAGTTTTAT
    GCTGATGCGG CAGAAGCTGA GGCCTGGATG GGAGAGCAAG AGTTACATAT GATGTCTGAA
    GAAAAAGCCA AGGATGAGAT TGGTGCCCAG GCAATGCTGA AGAAACACTT AGTAATGGAA
    CAGGCTCTTG ATGACTATGC AGACAACATC CATCAGCTTG CCAATTCAAG CCGCGACATG
    GTCTCCAGAG GTCATCCAGA AAGTGATCGT ATTACCCTTC GTCAAGGGCA AGTGGACAAG
    CTATATGCCA GTCTTAAAGA CCTGGCAGAG GAGAGACAAG GAAAGCTACA AGAGCACCAC
    AGACTTTGCC AACTCAAGAG AGATGTCGAA GACCTTGAGC ACTGGATCTC TGAACGTGAG
    GTGGTTGCAG CTTCTCATGA ACTTGGGCAA GATTATGAGC ATGTAACGAT GCTGAGAGAC
    AAATTCCGTG AGTTCTCCCG TGACACCAGT AACATTGGTC AAGAGCGGGT GGATGCTGTC
    AATCTGATGG CAGATGATTT AATTTCATCT GGACATTCGG AAAATGCTAC TGTAGCAGAT
    TGGAAAGACC AGCTAAATGA GGCATGGGCT GATCTTCTTG AGATGATTGA CACTCGAAGT
    CAGATGCTGG CAGCCTCATA TGAACTTCAC CGTTTCTACC AGGATGCAAA AGAAACCCTA
    GGTCAGATCC AAGACAAGCA GAAACAAATA CCAGAGGAGA CAGGCAGAGA TCTGAATACA
    GCAGAAGCCA TGCAGCGCAT GCACACCGCT TTTGAACATG ATATCCAAGC CCTGAGTGCA
    CAGGTGAAGC AAGTCCAGGA AGATGCTTCT CGCCTCCAAA AAGCTTATGC TGGTGAAAAG
    GCAGCAGACA TTCATCGCCA TGAGCAGGCA GTATCGGAAG CTTGGGGAGA GTTGCAGAAT
    GCTAGTGGTG CCAGGCGGCA GCTTCTTCTT GACACTGTGG AGAAATTCCG TTTCTTCAGA
    ATGGTGCGAG ATCTCATGTT ATGGATGGAT GATATAAACC TGCTAATTGA TGCACAGGAG
    AAGCCAAGAG ATGTATCCTC AGCTGATTTA GTCATCCAAA ACCACCTAGG CATCAAGGCA
    GAAGTGGATG CGCGAGCTGA CAGCTTTACT ACATGCATAG ATCTTGGCAA CTCTCTCCTA
    GAGAAGAATC ATTATGCCTC TGACAAGATT TCAGAGAAAC TGGAGCAATT GCAGGAAAGA
    AGGCAGGAGA CTGAAAACAA ATGGAAAGAT AAAATGGCAT GGCTGAGAGT TGTCCACGAA
    GTGCTGATGT TTGGAAGAGA TGCAAGTGTA GCTGAGGCTT GGCTGGCCAG CCAGGAGCCC
    ATTGTCCGGT CGTCAGAACT TGGTGGCAAT GTCGATGAAG TAGAATCCCT GATAAAAAGA
    CACGAAGCCT TTCAGAAAGC AGTTTCTACA TGGGATGAGA GATTTGTTCT TCTTGAAAAA
    CTAACCACGT TAGAAGAAAT TGAAATGCGT CGGAAGCGGG AAGAGGAAGA AAGAAAGAAA
    AGACCACCAA CACCACCTCT ACCAGTAGAA GAGCCTCGAG AGGAAGTTGT AAAAACCCAA
    GTTTCCCAGA CTATTGAAGG GCAGACTGAT CCAGCCAATA TACTTCAAGA AAGGATAAAT
    GGCATGCAGA ATTCATTCCA GGAGCAAGTA TCTGTTAATG GACTGTGCAC AGAGTCTGAT
    TCTCCACAGC CATCGTCCGA TACACAGAGG TTAGAAGACA GCATCTCTGA AAATCAGGGT
    CCGGAACAGT CAGATGTAAC CAATGGAGCC AGGACTGAGG TTATTACCAA AGATGGAAGT
    CCAACACCTT CTCCACAACT TGTGGCTAGG CCAACCTTCT CCCCGATAAA CCTCCAGTAT
    TCTACTTTGC CATCTAGCCC CACTGAACCA TTGGCATCTG AGCAAATGGA AGGAATGCTG
    CACCGCAAGC AGGAAATGGA TTCTCACAAC AAGAAGGCTG CGAACAGGTC CTGGCAGAAT
    GTTTACTGTG CACTGAAAAG GGGCAGTCTG GGTTTCTACA AGGATGCTAA AAGTGCAGCC
    AGTGGCGTTT CTTATCATTC GGAGCCCTCC GTCCAACTTA ATGGAGCCCA GTGCAGTGTT
    GCTCATGACT ACAAGAAACG CAAGAATGTC TTCAAGCTAA GCCTTCTTGA TGGCAAAGAA
    TATCTGTTCC AAGCTAAGGA TGAGGCTGAA ATGAGTAGTT GGATGCGAGT TATAAACGCC
    TCCCTAACTT CCTCCCCGGG AGACCCCTCA GACCATTCAC CGGGCATCAT GAAGGGTATG
    ACCCGGGCCA TGACAATGCC ACCAGTATCA CCCAACAGTG GTACAGAGGG ATCAGTCACA
    TTGCGCAACA AGGACGGGAA AGAAAAAGAC CGGGAAAAGA GATTCAGTTT CTTTAAGAAG
    AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004913752.1
    CDS238..7443
    Translation

    Target ORF information:

    RefSeq Version XM_004913695.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004913695.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
    GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA
    AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGATG AAAGAGAAGC TGTGCAGAAG
    AAGACATTTA CTAAATGGGT CAACTCTCAC TTGGCGAGGG TCACCTGTAG AATTTCTGAT
    CTCTACAATG ATTTACGAGA TGGACGGATG TTAATTCGAC TGTTAGAAGT GCTATCTGGA
    GAGCAGCTGC CAAAACCCAC AAAAGGTCGA ATGCGAATCC ACTGTTTGGA AAATGTAGAC
    AAAGCCCTTC AATTTTTAAA AGAGCAGAAG GTACATTTGG AGAACATGGG ATCACATGAC
    ATTGTGGATG GAAACCATCG ATTAACCCTA GGGTTAATCT GGACTATTAT TTTAAGGTTT
    CAGATCCAAG ATATCAGTGT AGAGACAGAA GATAATAAAG AAAAGAAGTC TGCTAAAGAT
    GCCTTACTGC TTTGGTGCCA GATGAAGACT GCTGGGTATC CAAATGTCAA TGTTCACAAC
    TTTACCACCA GCTGGCGAGA TGGACTTGCG TTTAATGCCA TTGTACACAA GCACAGACCA
    GATATAATTG ACTTTGACAC ACTGAAGAAG TCTAATGCAC ACCACAACCT CCAGAATGCC
    TTCAATGTAG CTGAAAGAGA GTTAGGTCTT ACTAAACTTC TGGATCCTGA AGATGTTAAT
    GTGGATCAGC CTGATGAGAA ATCCATCATA ACCTATGTGG CCACATTTTA TCACTATTTT
    TCCAAAATGA AAGCCCTAGC TGTGGAAGGA AAGCGTATTG GAAAGGTACT TGACCAAGCT
    ATTGAAGCTG ATCAGTTAAT TGAGAACTAT GAAGGACTGG CATCAGAACT CCTGCAATGG
    ATTGAACAGA TGATCATTAC TCTCAATGAC CGAAAACTGG CAAACTCTCT AAGTGGTGTT
    CAGAACCAGC TGCAGGCCTT CAACACCTAC AGGACTGTAG AAAAGCCGCC CAAGTTCACA
    GAAAAGGGCA ATTTGGAAGT TCTTCTCTTT ACCATACAGA GTAAAATGAG AGCTAATAAT
    CAAAAAGTGT ATACGCCCAG AGAAGGAAAG CTTATATCTG ATATAAACAA GGCATGGGAG
    CGACTAGAAA AGGCAGAGCA TGAACGTGAG CTTGCCCTTC GCAATGAACT TATACGCCAG
    GAAAAATTAG AGCAGTTGGC TGCACGATTT GACCGTAAAG CAGCCATGAG GGAAACCTGG
    CTAAGTGAAA ATCAGAGACT TGTAGCTCAG GATAACTTCG GTGTGGACTT AACTGCGGTG
    GAGGCAGCGC TACGGAAACA TGAAGCTATT GAAACTGATA TATTGGCTTA CAGTGAGCGC
    GTGCAAGCAG TGAATGCAGT GGCTGATGAG CTGGAAGCAG AGAATTACCA TGATGTCCGG
    AGGATTTTGG CTCGGAAGTG CAATGTGATT CGTCTCTCTG ATTATTTAAG AGAGCTTGTC
    ACTGCCAGGA GGCAAAGGCT TCATATGAAC CTAGAATTGC AGAGGATGTT CCAGGATTTA
    CATTATCTCA TGGACTGGAC TGAAGATATG AAGGGCCGTC TGCAGTCAGA AGATTATGGC
    AAGCATCTTC ATGGCGTGGA AGACCTTCTC CAGACTCACA CTCTTGTGGA GGCAGACATT
    GCAGTTCAGG CAGAACGTGT GAAATCAGTT ACAGCTGTAG CTCAGAAATT TATGACAGAA
    GATGAAGGCT ACAAGCCTTG CGACCCAGAG CTAGTGCGCA ACCGGGTGCA TCTCTTGGAA
    CAGCGCTATG CAGAGCTGTG TAATCTTTCT GCGGCTCGGA GATCAAAGCT GGAAGAATCC
    CGCCGTCTCT GGAAATTTTT CTGGGACATG GGAGAGGAGG AAGCCTGGAT CCGTGAGCAG
    AGTAGATTAC TACTTTCTGA TGACTATGGG AAAGACCTTA CCAGCTCACT TAGGTTGATC
    AGCAAGCATA ATGCCTTCAG AGGTGAGATG AGTGGACACC GTGGGCCGTT GCAGCAGACT
    GTGGCTGAGG GTGAGGAACT AATATCCAAA GGTCACTTTG GTGCAGGAGA GATTGAGGAA
    AGGATAAAAG AAGTGAAAGG CCAGTGGACA GAGATGGAGG CCTTAGCTGA AGACAGGGAA
    AGGAGGCTAA GAGAGGCCTA CAGCTATTAT CAGTTTCAAG CTGATGCCAA TGATGCTGAG
    GCTTGGATCC TGGACACTTT ACGCCTTGTT TCTACCAGTG AAGTAGGACA TGATGAGTAT
    TCCACACAGA CACTGGTGAA AAAGCACAAA GAAATAGAAG AGGAGATCCT TAGCCACCGA
    CCAGCTATTG ATGCGCTGCA TGAACAGGCC CGTGGCCTTC CACCTGCTTA CTCTCATTCA
    CCTGAAGTTG ATGGAAGGTT ACCAGCCCTG GAGCAGCGCT ATGAGGAATT GGTGGCTCTT
    GCTGCTCACC GCAAGCAGGC TCTGCAAGAT TCTCTGGCTC TTCATAAAAT GTTTAGTGAA
    GCTGATGCTT GCCTTTTGTG GATTGATGAG AAAGAACAGT GGCTTAATGT CACAGAGATT
    CCAGAAAAAC TTGAGGACCT AGAAGTAGTT CAACAAAGGT TTGAAACTCT AGAGGCAGAG
    ATGAATAATT TGGCATCCCG GATTGCATCT GTAAATGAAG TTGCTGGACA GTTGCTGACT
    ACAGACCAGA GAAATGAAAA CAGCATCCGA GACATGCAAG ATCAACTTAA TTCTCGGTGG
    AATGAATTCC GCAAGCTTGT TGACCAAAAG AAGGGTGCTT TGGATTCTGC TCTGGATATA
    CAGAATTATC ATTTGGAGTG CAATGAGACT GTGTGCTGGA TGAGGGAGAA GACAAAGGTT
    ATTGAATCCA CACAGAGCCT AGGGAATGAT CTTGCAGGAG TAACAGCCCT CCAGAGAAAA
    CTTACTGGAA TGGAGCGTGA TCTGAAAGCC ATTCAGGGAA AAGTGGGTGA CCTGCGTTCA
    GAGGCAGAAA AGCTTGCAGA TGGGCACCCA CAGAAAGCAC CGGCCATAAT TGGCCGCTTA
    TCAGATATTG ATGAGACCTG GGGAGAACTA AAGGACACTA TGAAAAGAAG AGAAGAAACT
    TTAGGTGAAG CAAGCAAGCT GCAGGGATTT TTGAGAGACC TTGATGATTT CCAGAGCTGG
    TTGTCCCGGG CCCAGACAGC AGTGGCATCT GAGGATGTTC CAGCTGACCT AGCAGAAGCA
    GAGCGCCTTC TTGCACAACA TGAAGGCATA AAAAATGAGA TTGATCATTA TGGAGCTGAC
    TACCACAGAG TGAGAGATGT TGGTGAAGAA GTCACTCGTG GGCAAACAGA TGCCCAATAT
    ATGTTCTTGC AACAGAGACT GAAGGCGCTA GACACTGGCT GGAATGAGCT TGGACAGATG
    TGGGAAAACA GGCACCAGTT GTTGTCCCAG GGTCACAGCT TCCAGCAGTT TTTGAAAGAT
    ACAAAACAAG TGGAGGGGTT CCTTAATAAC CAGGAGTATG TCCTTTCCCA TACCGAAATG
    CCAAGTACGC TACAGGCAGC TGAAGCTGCC ATCAAACGCC ATGAAGATTT CCTCACAACC
    ATGGATGCCA ATGAAGAGAA GATACAGGGT CTTGTAGAAT CTGGGAGGAA GCTAATTGAG
    GAACAGAATG TCAATTCTGA GAAGATTCAA GAAAAAGTCA CTTCTATTGA TGAGAGACAC
    AAAAAGAACC AAAAAACTGC TAGCGAAGTA TTAACTCGTT TGAAGGACAA CCGTGATCTT
    CAACATTTCC TTCAAGACTG TCAAGAGCTC ACCCTCTGGA TCAATGAAAA AATGCTTACT
    GCTCAAGATG TTTCTTATGA TGAAGCTCGA AACCTGCATA GCAAGTGGCA AAAGCATCAG
    GCATTCATGG CAGAACTGGC CTCAAATAAA GACTGGCTTG ACAAAATAGA CAAGGAAGGG
    CAGCAGCTCA TGTCAGAAAA ACCGGAGCTT GAACCAGTAG TGAGAGAAAA ACTAGACACT
    CTGCATAAGC TGTGGAATGA CTTGGAATCT ACCACACAAA GTAAAGCTCA GTGTCTGTTT
    GATGCCAACA GGGCAGAGTT ATTCACACAA AGCTGTTCTG CTCTGGAGAG TTGGCTTCAA
    AACCTCGAGT CACAACTACA GTCAGATCAT TATGGCAAAG ATCTGACCAG TGTCAACATC
    CTATTAAATA AACAGCAGAT GCTGGAGAAC CAAATGGAAG TGAGGGAGAA GGAGCTAGAA
    GCTATACAGT CCCAGGCACA GGCCCTTGGA CAGGAGGATG CCAACATAGT GGAGGTTGAC
    TCAAAGCATA AGGATGTAGC AGATAAATTT TCCAAGCTGC GTGCTCCTCT AAACAAGAGA
    AGAAAATTAC TTCTAGCCTC CAAGGAAGGA CATCAGTTTA ACAGGGACCT GGAAGATGAA
    ATACTTTGGG TAAAGGAGAA GATGCCAATG GCAATCTCCA CTGATCATGG AAAAGATCTC
    CCGACAGTCC AGCTTCTTAT CAAAAAGAAT CAGACACTCC AAAAGGAAAT TTTGGGACAT
    CAACCACGGA TTGATGACAT CCTGGAGAGA GGCAAATCTA TGATCCAAGA CAGCTCTCTG
    GACCCTCAAG AGATAGAAGA AAGGCTTGGG CTACTGAAAA GCACATGGGA AGAGCTGGCA
    GCAGAAGGAG GAAAGCGCCA TGCCCGTTTA GAGGAATCTC TTGCTGCTCA GCAGTTTTAT
    GCTGATGCGG CAGAAGCTGA GGCCTGGATG GGAGAGCAAG AGTTACATAT GATGTCTGAA
    GAAAAAGCCA AGGATGAGAT TGGTGCCCAG GCAATGCTGA AGAAACACTT AGTAATGGAA
    CAGGCTCTTG ATGACTATGC AGACAACATC CATCAGCTTG CCAATTCAAG CCGCGACATG
    GTCTCCAGAG GTCATCCAGA AAGTGATCGT ATTACCCTTC GTCAAGGGCA AGTGGACAAG
    CTATATGCCA GTCTTAAAGA CCTGGCAGAG GAGAGACAAG GAAAGCTACA AGAGCACCAC
    AGACTTTGCC AACTCAAGAG AGATGTCGAA GACCTTGAGC ACTGGATCTC TGAACGTGAG
    GTGGTTGCAG CTTCTCATGA ACTTGGGCAA GATTATGAGC ATGTAACGAT GCTGAGAGAC
    AAATTCCGTG AGTTCTCCCG TGACACCAGT AACATTGGTC AAGAGCGGGT GGATGCTGTC
    AATCTGATGG CAGATGATTT AATTTCATCT GGACATTCGG AAAATGCTAC TGTAGCAGAT
    TGGAAAGACC AGCTAAATGA GGCATGGGCT GATCTTCTTG AGATGATTGA CACTCGAAGT
    CAGATGCTGG CAGCCTCATA TGAACTTCAC CGTTTCTACC AGGATGCAAA AGAAACCCTA
    GGTCAGATCC AAGACAAGCA GAAACAAATA CCAGAGGAGA CAGGCAGAGA TCTGAATACA
    GCAGAAGCCA TGCAGCGCAT GCACACCGCT TTTGAACATG ATATCCAAGC CCTGAGTGCA
    CAGGTGAAGC AAGTCCAGGA AGATGCTTCT CGCCTCCAAA AAGCTTATGC TGGTGAAAAG
    GCAGCAGACA TTCATCGCCA TGAGCAGGCA GTATCGGAAG CTTGGGGAGA GTTGCAGAAT
    GCTAGTGGTG CCAGGCGGCA GCTTCTTCTT GACACTGTGG AGAAATTCCG TTTCTTCAGA
    ATGGTGCGAG ATCTCATGTT ATGGATGGAT GATATAAACC TGCTAATTGA TGCACAGGAG
    AAGCCAAGAG ATGTATCCTC AGCTGATTTA GTCATCCAAA ACCACCTAGG CATCAAGGCA
    GAAGTGGATG CGCGAGCTGA CAGCTTTACT ACATGCATAG ATCTTGGCAA CTCTCTCCTA
    GAGAAGAATC ATTATGCCTC TGACAAGATT TCAGAGAAAC TGGAGCAATT GCAGGAAAGA
    AGGCAGGAGA CTGAAAACAA ATGGAAAGAT AAAATGGCAT GGCTGAGAGT TGTCCACGAA
    GTGCTGATGT TTGGAAGAGA TGCAAGTGTA GCTGAGGCTT GGCTGGCCAG CCAGGAGCCC
    ATTGTCCGGT CGTCAGAACT TGGTGGCAAT GTCGATGAAG TAGAATCCCT GATAAAAAGA
    CACGAAGCCT TTCAGAAAGC AGTTTCTACA TGGGATGAGA GATTTGTTCT TCTTGAAAAA
    CTAACCACGT TAGAAGAAAT TGAAATGCGT CGGAAGCGGG AAGAGGAAGA AAGAAAGAAA
    AGACCACCAA CACCACCTCT ACCAGTAGAA GAGCCTCGAG AGGAAGTTGT AAAAACCCAA
    GTTTCCCAGA CTATTGAAGG GCAGACTGAT CCAGCCAATA TACTTCAAGA AAGGATAAAT
    GGCATGCAGA ATTCATTCCA GGAGCAAGTA TCTGTTAATG GACTGTGCAC AGAGTCTGAT
    TCTCCACAGC CATCGTCCGA TACACAGAGG TTAGAAGACA GCATCTCTGA AAATCAGGGT
    CCGGAACAGT CAGATGTAAC CAATGGAGCC AGGACTGAGG TTATTACCAA AGATGGAAGT
    CCAACACCTT CTCCACAACT TGTGGCTAGG CCAACCTTCT CCCCGATAAA CCTCCAGTAT
    TCTACTTTGC CATCTAGCCC CACTGAACCA TTGGCATCTG AGCAAATGGA AGGAATGCTG
    CACCGCAAGC AGGAAATGGA TTCTCACAAC AAGAAGGCTG CGAACAGGTC CTGGCAGAAT
    GTTTACTGTG CACTGAAAAG GGGCAGTCTG GGTTTCTACA AGGATGCTAA AAGTGCAGCC
    AGTGGCGTTT CTTATCATTC GGAGCCCTCC GTCCAACTTA ATGGAGCCCA GTGCAGTGTT
    GCTCATGACT ACAAGAAACG CAAGAATGTC TTCAAGCTAA GCCTTCTTGA TGGCAAAGAA
    TATCTGTTCC AAGCTAAGGA TGAGGCTGAA ATGAGTAGTT GGATGCGAGT TATAAACGCC
    TCCCTAACTT CCTCCCCGGG AGACCCCTCA GACCATTCAC CGGGCATCAT GAAGGGTATG
    ACCCGGGCCA TGACAATGCC ACCAGTATCA CCCAACAGTG GTACAGAGGG ATCAGTCACA
    TTGCGCAACA AGGACGGGAA AGAAAAAGAC CGGGAAAAGA GATTCAGTTT CTTTAAGAAG
    AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa56731
    Clone ID Related Accession (Same CDS sequence) XM_012962405.3
    Accession Version XM_012962405.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin beta chain, non-erythrocytic 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012962405.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
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    3841
    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
    GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA
    AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGAAA TGGATTTTGT CCCCCGATCT
    GGCTCCCCTC TCCTATCCCC TGCGGCTTTT GTAAATGCAG TGCAATACGC TAACGTGCTG
    GAAGGAAGGT TCAAGCAGCT GCAAGATGAA AGAGAAGCTG TGCAGAAGAA GACATTTACT
    AAATGGGTCA ACTCTCACTT GGCGAGGGTC ACCTGTAGAA TTTCTGATCT CTACAATGAT
    TTACGAGATG GACGGATGTT AATTCGACTG TTAGAAGTGC TATCTGGAGA GCAGCTGCCA
    AAACCCACAA AAGGTCGAAT GCGAATCCAC TGTTTGGAAA ATGTAGACAA AGCCCTTCAA
    TTTTTAAAAG AGCAGAAGGT ACATTTGGAG AACATGGGAT CACATGACAT TGTGGATGGA
    AACCATCGAT TAACCCTAGG GTTAATCTGG ACTATTATTT TAAGGTTTCA GATCCAAGAT
    ATCAGTGTAG AGACAGAAGA TAATAAAGAA AAGAAGTCTG CTAAAGATGC CTTACTGCTT
    TGGTGCCAGA TGAAGACTGC TGGGTATCCA AATGTCAATG TTCACAACTT TACCACCAGC
    TGGCGAGATG GACTTGCGTT TAATGCCATT GTACACAAGC ACAGACCAGA TATAATTGAC
    TTTGACACAC TGAAGAAGTC TAATGCACAC CACAACCTCC AGAATGCCTT CAATGTAGCT
    GAAAGAGAGT TAGGTCTTAC TAAACTTCTG GATCCTGAAG ATGTTAATGT GGATCAGCCT
    GATGAGAAAT CCATCATAAC CTATGTGGCC ACATTTTATC ACTATTTTTC CAAAATGAAA
    GCCCTAGCTG TGGAAGGAAA GCGTATTGGA AAGGTACTTG ACCAAGCTAT TGAAGCTGAT
    CAGTTAATTG AGAACTATGA AGGACTGGCA TCAGAACTCC TGCAATGGAT TGAACAGATG
    ATCATTACTC TCAATGACCG AAAACTGGCA AACTCTCTAA GTGGTGTTCA GAACCAGCTG
    CAGGCCTTCA ACACCTACAG GACTGTAGAA AAGCCGCCCA AGTTCACAGA AAAGGGCAAT
    TTGGAAGTTC TTCTCTTTAC CATACAGAGT AAAATGAGAG CTAATAATCA AAAAGTGTAT
    ACGCCCAGAG AAGGAAAGCT TATATCTGAT ATAAACAAGG CATGGGAGCG ACTAGAAAAG
    GCAGAGCATG AACGTGAGCT TGCCCTTCGC AATGAACTTA TACGCCAGGA AAAATTAGAG
    CAGTTGGCTG CACGATTTGA CCGTAAAGCA GCCATGAGGG AAACCTGGCT AAGTGAAAAT
    CAGAGACTTG TAGCTCAGGA TAACTTCGGT GTGGACTTAA CTGCGGTGGA GGCAGCGCTA
    CGGAAACATG AAGCTATTGA AACTGATATA TTGGCTTACA GTGAGCGCGT GCAAGCAGTG
    AATGCAGTGG CTGATGAGCT GGAAGCAGAG AATTACCATG ATGTCCGGAG GATTTTGGCT
    CGGAAGTGCA ATGTGATTCG TCTCTCTGAT TATTTAAGAG AGCTTGTCAC TGCCAGGAGG
    CAAAGGCTTC ATATGAACCT AGAATTGCAG AGGATGTTCC AGGATTTACA TTATCTCATG
    GACTGGACTG AAGATATGAA GGGCCGTCTG CAGTCAGAAG ATTATGGCAA GCATCTTCAT
    GGCGTGGAAG ACCTTCTCCA GACTCACACT CTTGTGGAGG CAGACATTGC AGTTCAGGCA
    GAACGTGTGA AATCAGTTAC AGCTGTAGCT CAGAAATTTA TGACAGAAGA TGAAGGCTAC
    AAGCCTTGCG ACCCAGAGCT AGTGCGCAAC CGGGTGCATC TCTTGGAACA GCGCTATGCA
    GAGCTGTGTA ATCTTTCTGC GGCTCGGAGA TCAAAGCTGG AAGAATCCCG CCGTCTCTGG
    AAATTTTTCT GGGACATGGG AGAGGAGGAA GCCTGGATCC GTGAGCAGAG TAGATTACTA
    CTTTCTGATG ACTATGGGAA AGACCTTACC AGCTCACTTA GGTTGATCAG CAAGCATAAT
    GCCTTCAGAG GTGAGATGAG TGGACACCGT GGGCCGTTGC AGCAGACTGT GGCTGAGGGT
    GAGGAACTAA TATCCAAAGG TCACTTTGGT GCAGGAGAGA TTGAGGAAAG GATAAAAGAA
    GTGAAAGGCC AGTGGACAGA GATGGAGGCC TTAGCTGAAG ACAGGGAAAG GAGGCTAAGA
    GAGGCCTACA GCTATTATCA GTTTCAAGCT GATGCCAATG ATGCTGAGGC TTGGATCCTG
    GACACTTTAC GCCTTGTTTC TACCAGTGAA GTAGGACATG ATGAGTATTC CACACAGACA
    CTGGTGAAAA AGCACAAAGA AATAGAAGAG GAGATCCTTA GCCACCGACC AGCTATTGAT
    GCGCTGCATG AACAGGCCCG TGGCCTTCCA CCTGCTTACT CTCATTCACC TGAAGTTGAT
    GGAAGGTTAC CAGCCCTGGA GCAGCGCTAT GAGGAATTGG TGGCTCTTGC TGCTCACCGC
    AAGCAGGCTC TGCAAGATTC TCTGGCTCTT CATAAAATGT TTAGTGAAGC TGATGCTTGC
    CTTTTGTGGA TTGATGAGAA AGAACAGTGG CTTAATGTCA CAGAGATTCC AGAAAAACTT
    GAGGACCTAG AAGTAGTTCA ACAAAGGTTT GAAACTCTAG AGGCAGAGAT GAATAATTTG
    GCATCCCGGA TTGCATCTGT AAATGAAGTT GCTGGACAGT TGCTGACTAC AGACCAGAGA
    AATGAAAACA GCATCCGAGA CATGCAAGAT CAACTTAATT CTCGGTGGAA TGAATTCCGC
    AAGCTTGTTG ACCAAAAGAA GGGTGCTTTG GATTCTGCTC TGGATATACA GAATTATCAT
    TTGGAGTGCA ATGAGACTGT GTGCTGGATG AGGGAGAAGA CAAAGGTTAT TGAATCCACA
    CAGAGCCTAG GGAATGATCT TGCAGGAGTA ACAGCCCTCC AGAGAAAACT TACTGGAATG
    GAGCGTGATC TGAAAGCCAT TCAGGGAAAA GTGGGTGACC TGCGTTCAGA GGCAGAAAAG
    CTTGCAGATG GGCACCCACA GAAAGCACCG GCCATAATTG GCCGCTTATC AGATATTGAT
    GAGACCTGGG GAGAACTAAA GGACACTATG AAAAGAAGAG AAGAAACTTT AGGTGAAGCA
    AGCAAGCTGC AGGGATTTTT GAGAGACCTT GATGATTTCC AGAGCTGGTT GTCCCGGGCC
    CAGACAGCAG TGGCATCTGA GGATGTTCCA GCTGACCTAG CAGAAGCAGA GCGCCTTCTT
    GCACAACATG AAGGCATAAA AAATGAGATT GATCATTATG GAGCTGACTA CCACAGAGTG
    AGAGATGTTG GTGAAGAAGT CACTCGTGGG CAAACAGATG CCCAATATAT GTTCTTGCAA
    CAGAGACTGA AGGCGCTAGA CACTGGCTGG AATGAGCTTG GACAGATGTG GGAAAACAGG
    CACCAGTTGT TGTCCCAGGG TCACAGCTTC CAGCAGTTTT TGAAAGATAC AAAACAAGTG
    GAGGGGTTCC TTAATAACCA GGAGTATGTC CTTTCCCATA CCGAAATGCC AAGTACGCTA
    CAGGCAGCTG AAGCTGCCAT CAAACGCCAT GAAGATTTCC TCACAACCAT GGATGCCAAT
    GAAGAGAAGA TACAGGGTCT TGTAGAATCT GGGAGGAAGC TAATTGAGGA ACAGAATGTC
    AATTCTGAGA AGATTCAAGA AAAAGTCACT TCTATTGATG AGAGACACAA AAAGAACCAA
    AAAACTGCTA GCGAAGTATT AACTCGTTTG AAGGACAACC GTGATCTTCA ACATTTCCTT
    CAAGACTGTC AAGAGCTCAC CCTCTGGATC AATGAAAAAA TGCTTACTGC TCAAGATGTT
    TCTTATGATG AAGCTCGAAA CCTGCATAGC AAGTGGCAAA AGCATCAGGC ATTCATGGCA
    GAACTGGCCT CAAATAAAGA CTGGCTTGAC AAAATAGACA AGGAAGGGCA GCAGCTCATG
    TCAGAAAAAC CGGAGCTTGA ACCAGTAGTG AGAGAAAAAC TAGACACTCT GCATAAGCTG
    TGGAATGACT TGGAATCTAC CACACAAAGT AAAGCTCAGT GTCTGTTTGA TGCCAACAGG
    GCAGAGTTAT TCACACAAAG CTGTTCTGCT CTGGAGAGTT GGCTTCAAAA CCTCGAGTCA
    CAACTACAGT CAGATCATTA TGGCAAAGAT CTGACCAGTG TCAACATCCT ATTAAATAAA
    CAGCAGATGC TGGAGAACCA AATGGAAGTG AGGGAGAAGG AGCTAGAAGC TATACAGTCC
    CAGGCACAGG CCCTTGGACA GGAGGATGCC AACATAGTGG AGGTTGACTC AAAGCATAAG
    GATGTAGCAG ATAAATTTTC CAAGCTGCGT GCTCCTCTAA ACAAGAGAAG AAAATTACTT
    CTAGCCTCCA AGGAAGGACA TCAGTTTAAC AGGGACCTGG AAGATGAAAT ACTTTGGGTA
    AAGGAGAAGA TGCCAATGGC AATCTCCACT GATCATGGAA AAGATCTCCC GACAGTCCAG
    CTTCTTATCA AAAAGAATCA GACACTCCAA AAGGAAATTT TGGGACATCA ACCACGGATT
    GATGACATCC TGGAGAGAGG CAAATCTATG ATCCAAGACA GCTCTCTGGA CCCTCAAGAG
    ATAGAAGAAA GGCTTGGGCT ACTGAAAAGC ACATGGGAAG AGCTGGCAGC AGAAGGAGGA
    AAGCGCCATG CCCGTTTAGA GGAATCTCTT GCTGCTCAGC AGTTTTATGC TGATGCGGCA
    GAAGCTGAGG CCTGGATGGG AGAGCAAGAG TTACATATGA TGTCTGAAGA AAAAGCCAAG
    GATGAGATTG GTGCCCAGGC AATGCTGAAG AAACACTTAG TAATGGAACA GGCTCTTGAT
    GACTATGCAG ACAACATCCA TCAGCTTGCC AATTCAAGCC GCGACATGGT CTCCAGAGGT
    CATCCAGAAA GTGATCGTAT TACCCTTCGT CAAGGGCAAG TGGACAAGCT ATATGCCAGT
    CTTAAAGACC TGGCAGAGGA GAGACAAGGA AAGCTACAAG AGCACCACAG ACTTTGCCAA
    CTCAAGAGAG ATGTCGAAGA CCTTGAGCAC TGGATCTCTG AACGTGAGGT GGTTGCAGCT
    TCTCATGAAC TTGGGCAAGA TTATGAGCAT GTAACGATGC TGAGAGACAA ATTCCGTGAG
    TTCTCCCGTG ACACCAGTAA CATTGGTCAA GAGCGGGTGG ATGCTGTCAA TCTGATGGCA
    GATGATTTAA TTTCATCTGG ACATTCGGAA AATGCTACTG TAGCAGATTG GAAAGACCAG
    CTAAATGAGG CATGGGCTGA TCTTCTTGAG ATGATTGACA CTCGAAGTCA GATGCTGGCA
    GCCTCATATG AACTTCACCG TTTCTACCAG GATGCAAAAG AAACCCTAGG TCAGATCCAA
    GACAAGCAGA AACAAATACC AGAGGAGACA GGCAGAGATC TGAATACAGC AGAAGCCATG
    CAGCGCATGC ACACCGCTTT TGAACATGAT ATCCAAGCCC TGAGTGCACA GGTGAAGCAA
    GTCCAGGAAG ATGCTTCTCG CCTCCAAAAA GCTTATGCTG GTGAAAAGGC AGCAGACATT
    CATCGCCATG AGCAGGCAGT ATCGGAAGCT TGGGGAGAGT TGCAGAATGC TAGTGGTGCC
    AGGCGGCAGC TTCTTCTTGA CACTGTGGAG AAATTCCGTT TCTTCAGAAT GGTGCGAGAT
    CTCATGTTAT GGATGGATGA TATAAACCTG CTAATTGATG CACAGGAGAA GCCAAGAGAT
    GTATCCTCAG CTGATTTAGT CATCCAAAAC CACCTAGGCA TCAAGGCAGA AGTGGATGCG
    CGAGCTGACA GCTTTACTAC ATGCATAGAT CTTGGCAACT CTCTCCTAGA GAAGAATCAT
    TATGCCTCTG ACAAGATTTC AGAGAAACTG GAGCAATTGC AGGAAAGAAG GCAGGAGACT
    GAAAACAAAT GGAAAGATAA AATGGCATGG CTGAGAGTTG TCCACGAAGT GCTGATGTTT
    GGAAGAGATG CAAGTGTAGC TGAGGCTTGG CTGGCCAGCC AGGAGCCCAT TGTCCGGTCG
    TCAGAACTTG GTGGCAATGT CGATGAAGTA GAATCCCTGA TAAAAAGACA CGAAGCCTTT
    CAGAAAGCAG TTTCTACATG GGATGAGAGA TTTGTTCTTC TTGAAAAACT AACCACGTTA
    GAAGAAATTG AAATGCGTCG GAAGCGGGAA GAGGAAGAAA GAAAGAAAAG ACCACCAACA
    CCACCTCTAC CAGTAGAAGA GCCTCGAGAG GAAGTTGTAA AAACCCAAGT TTCCCAGACT
    ATTGAAGGGC AGACTGATCC AGCCAATATA CTTCAAGAAA GGATAAATGG CATGCAGAAT
    TCATTCCAGG AGCAAGTATC TGTTAATGGA CTGTGCACAG AGTCTGATTC TCCACAGCCA
    TCGTCCGATA CACAGAGGTT AGAAGACAGC ATCTCTGAAA ATCAGGGTCC GGAACAGTCA
    GATGTAACCA ATGGAGCCAG GACTGAGGTT ATTACCAAAG ATGGAAGTCC AACACCTTCT
    CCACAACTTG TGGCTAGGCC AACCTTCTCC CCGATAAACC TCCAGTATTC TACTTTGCCA
    TCTAGCCCCA CTGAACCATT GGCATCTGAG CAAATGGAAG GAATGCTGCA CCGCAAGCAG
    GAAATGGATT CTCACAACAA GAAGGCTGCG AACAGGTCCT GGCAGAATGT TTACTGTGCA
    CTGAAAAGGG GCAGTCTGGG TTTCTACAAG GATGCTAAAA GTGCAGCCAG TGGCGTTTCT
    TATCATTCGG AGCCCTCCGT CCAACTTAAT GGAGCCCAGT GCAGTGTTGC TCATGACTAC
    AAGAAACGCA AGAATGTCTT CAAGCTAAGC CTTCTTGATG GCAAAGAATA TCTGTTCCAA
    GCTAAGGATG AGGCTGAAAT GAGTAGTTGG ATGCGAGTTA TAAACGCCTC CCTAACTTCC
    TCCCCGGGAG ACCCCTCAGA CCATTCACCG GGCATCATGA AGGGTATGAC CCGGGCCATG
    ACAATGCCAC CAGTATCACC CAACAGTGGT ACAGAGGGAT CAGTCACATT GCGCAACAAG
    GACGGGAAAG AAAAAGACCG GGAAAAGAGA TTCAGTTTCT TTAAGAAGAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817859.1
    CDS238..7551
    Translation

    Target ORF information:

    RefSeq Version XM_012962405.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962405.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
    GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA
    AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGAAA TGGATTTTGT CCCCCGATCT
    GGCTCCCCTC TCCTATCCCC TGCGGCTTTT GTAAATGCAG TGCAATACGC TAACGTGCTG
    GAAGGAAGGT TCAAGCAGCT GCAAGATGAA AGAGAAGCTG TGCAGAAGAA GACATTTACT
    AAATGGGTCA ACTCTCACTT GGCGAGGGTC ACCTGTAGAA TTTCTGATCT CTACAATGAT
    TTACGAGATG GACGGATGTT AATTCGACTG TTAGAAGTGC TATCTGGAGA GCAGCTGCCA
    AAACCCACAA AAGGTCGAAT GCGAATCCAC TGTTTGGAAA ATGTAGACAA AGCCCTTCAA
    TTTTTAAAAG AGCAGAAGGT ACATTTGGAG AACATGGGAT CACATGACAT TGTGGATGGA
    AACCATCGAT TAACCCTAGG GTTAATCTGG ACTATTATTT TAAGGTTTCA GATCCAAGAT
    ATCAGTGTAG AGACAGAAGA TAATAAAGAA AAGAAGTCTG CTAAAGATGC CTTACTGCTT
    TGGTGCCAGA TGAAGACTGC TGGGTATCCA AATGTCAATG TTCACAACTT TACCACCAGC
    TGGCGAGATG GACTTGCGTT TAATGCCATT GTACACAAGC ACAGACCAGA TATAATTGAC
    TTTGACACAC TGAAGAAGTC TAATGCACAC CACAACCTCC AGAATGCCTT CAATGTAGCT
    GAAAGAGAGT TAGGTCTTAC TAAACTTCTG GATCCTGAAG ATGTTAATGT GGATCAGCCT
    GATGAGAAAT CCATCATAAC CTATGTGGCC ACATTTTATC ACTATTTTTC CAAAATGAAA
    GCCCTAGCTG TGGAAGGAAA GCGTATTGGA AAGGTACTTG ACCAAGCTAT TGAAGCTGAT
    CAGTTAATTG AGAACTATGA AGGACTGGCA TCAGAACTCC TGCAATGGAT TGAACAGATG
    ATCATTACTC TCAATGACCG AAAACTGGCA AACTCTCTAA GTGGTGTTCA GAACCAGCTG
    CAGGCCTTCA ACACCTACAG GACTGTAGAA AAGCCGCCCA AGTTCACAGA AAAGGGCAAT
    TTGGAAGTTC TTCTCTTTAC CATACAGAGT AAAATGAGAG CTAATAATCA AAAAGTGTAT
    ACGCCCAGAG AAGGAAAGCT TATATCTGAT ATAAACAAGG CATGGGAGCG ACTAGAAAAG
    GCAGAGCATG AACGTGAGCT TGCCCTTCGC AATGAACTTA TACGCCAGGA AAAATTAGAG
    CAGTTGGCTG CACGATTTGA CCGTAAAGCA GCCATGAGGG AAACCTGGCT AAGTGAAAAT
    CAGAGACTTG TAGCTCAGGA TAACTTCGGT GTGGACTTAA CTGCGGTGGA GGCAGCGCTA
    CGGAAACATG AAGCTATTGA AACTGATATA TTGGCTTACA GTGAGCGCGT GCAAGCAGTG
    AATGCAGTGG CTGATGAGCT GGAAGCAGAG AATTACCATG ATGTCCGGAG GATTTTGGCT
    CGGAAGTGCA ATGTGATTCG TCTCTCTGAT TATTTAAGAG AGCTTGTCAC TGCCAGGAGG
    CAAAGGCTTC ATATGAACCT AGAATTGCAG AGGATGTTCC AGGATTTACA TTATCTCATG
    GACTGGACTG AAGATATGAA GGGCCGTCTG CAGTCAGAAG ATTATGGCAA GCATCTTCAT
    GGCGTGGAAG ACCTTCTCCA GACTCACACT CTTGTGGAGG CAGACATTGC AGTTCAGGCA
    GAACGTGTGA AATCAGTTAC AGCTGTAGCT CAGAAATTTA TGACAGAAGA TGAAGGCTAC
    AAGCCTTGCG ACCCAGAGCT AGTGCGCAAC CGGGTGCATC TCTTGGAACA GCGCTATGCA
    GAGCTGTGTA ATCTTTCTGC GGCTCGGAGA TCAAAGCTGG AAGAATCCCG CCGTCTCTGG
    AAATTTTTCT GGGACATGGG AGAGGAGGAA GCCTGGATCC GTGAGCAGAG TAGATTACTA
    CTTTCTGATG ACTATGGGAA AGACCTTACC AGCTCACTTA GGTTGATCAG CAAGCATAAT
    GCCTTCAGAG GTGAGATGAG TGGACACCGT GGGCCGTTGC AGCAGACTGT GGCTGAGGGT
    GAGGAACTAA TATCCAAAGG TCACTTTGGT GCAGGAGAGA TTGAGGAAAG GATAAAAGAA
    GTGAAAGGCC AGTGGACAGA GATGGAGGCC TTAGCTGAAG ACAGGGAAAG GAGGCTAAGA
    GAGGCCTACA GCTATTATCA GTTTCAAGCT GATGCCAATG ATGCTGAGGC TTGGATCCTG
    GACACTTTAC GCCTTGTTTC TACCAGTGAA GTAGGACATG ATGAGTATTC CACACAGACA
    CTGGTGAAAA AGCACAAAGA AATAGAAGAG GAGATCCTTA GCCACCGACC AGCTATTGAT
    GCGCTGCATG AACAGGCCCG TGGCCTTCCA CCTGCTTACT CTCATTCACC TGAAGTTGAT
    GGAAGGTTAC CAGCCCTGGA GCAGCGCTAT GAGGAATTGG TGGCTCTTGC TGCTCACCGC
    AAGCAGGCTC TGCAAGATTC TCTGGCTCTT CATAAAATGT TTAGTGAAGC TGATGCTTGC
    CTTTTGTGGA TTGATGAGAA AGAACAGTGG CTTAATGTCA CAGAGATTCC AGAAAAACTT
    GAGGACCTAG AAGTAGTTCA ACAAAGGTTT GAAACTCTAG AGGCAGAGAT GAATAATTTG
    GCATCCCGGA TTGCATCTGT AAATGAAGTT GCTGGACAGT TGCTGACTAC AGACCAGAGA
    AATGAAAACA GCATCCGAGA CATGCAAGAT CAACTTAATT CTCGGTGGAA TGAATTCCGC
    AAGCTTGTTG ACCAAAAGAA GGGTGCTTTG GATTCTGCTC TGGATATACA GAATTATCAT
    TTGGAGTGCA ATGAGACTGT GTGCTGGATG AGGGAGAAGA CAAAGGTTAT TGAATCCACA
    CAGAGCCTAG GGAATGATCT TGCAGGAGTA ACAGCCCTCC AGAGAAAACT TACTGGAATG
    GAGCGTGATC TGAAAGCCAT TCAGGGAAAA GTGGGTGACC TGCGTTCAGA GGCAGAAAAG
    CTTGCAGATG GGCACCCACA GAAAGCACCG GCCATAATTG GCCGCTTATC AGATATTGAT
    GAGACCTGGG GAGAACTAAA GGACACTATG AAAAGAAGAG AAGAAACTTT AGGTGAAGCA
    AGCAAGCTGC AGGGATTTTT GAGAGACCTT GATGATTTCC AGAGCTGGTT GTCCCGGGCC
    CAGACAGCAG TGGCATCTGA GGATGTTCCA GCTGACCTAG CAGAAGCAGA GCGCCTTCTT
    GCACAACATG AAGGCATAAA AAATGAGATT GATCATTATG GAGCTGACTA CCACAGAGTG
    AGAGATGTTG GTGAAGAAGT CACTCGTGGG CAAACAGATG CCCAATATAT GTTCTTGCAA
    CAGAGACTGA AGGCGCTAGA CACTGGCTGG AATGAGCTTG GACAGATGTG GGAAAACAGG
    CACCAGTTGT TGTCCCAGGG TCACAGCTTC CAGCAGTTTT TGAAAGATAC AAAACAAGTG
    GAGGGGTTCC TTAATAACCA GGAGTATGTC CTTTCCCATA CCGAAATGCC AAGTACGCTA
    CAGGCAGCTG AAGCTGCCAT CAAACGCCAT GAAGATTTCC TCACAACCAT GGATGCCAAT
    GAAGAGAAGA TACAGGGTCT TGTAGAATCT GGGAGGAAGC TAATTGAGGA ACAGAATGTC
    AATTCTGAGA AGATTCAAGA AAAAGTCACT TCTATTGATG AGAGACACAA AAAGAACCAA
    AAAACTGCTA GCGAAGTATT AACTCGTTTG AAGGACAACC GTGATCTTCA ACATTTCCTT
    CAAGACTGTC AAGAGCTCAC CCTCTGGATC AATGAAAAAA TGCTTACTGC TCAAGATGTT
    TCTTATGATG AAGCTCGAAA CCTGCATAGC AAGTGGCAAA AGCATCAGGC ATTCATGGCA
    GAACTGGCCT CAAATAAAGA CTGGCTTGAC AAAATAGACA AGGAAGGGCA GCAGCTCATG
    TCAGAAAAAC CGGAGCTTGA ACCAGTAGTG AGAGAAAAAC TAGACACTCT GCATAAGCTG
    TGGAATGACT TGGAATCTAC CACACAAAGT AAAGCTCAGT GTCTGTTTGA TGCCAACAGG
    GCAGAGTTAT TCACACAAAG CTGTTCTGCT CTGGAGAGTT GGCTTCAAAA CCTCGAGTCA
    CAACTACAGT CAGATCATTA TGGCAAAGAT CTGACCAGTG TCAACATCCT ATTAAATAAA
    CAGCAGATGC TGGAGAACCA AATGGAAGTG AGGGAGAAGG AGCTAGAAGC TATACAGTCC
    CAGGCACAGG CCCTTGGACA GGAGGATGCC AACATAGTGG AGGTTGACTC AAAGCATAAG
    GATGTAGCAG ATAAATTTTC CAAGCTGCGT GCTCCTCTAA ACAAGAGAAG AAAATTACTT
    CTAGCCTCCA AGGAAGGACA TCAGTTTAAC AGGGACCTGG AAGATGAAAT ACTTTGGGTA
    AAGGAGAAGA TGCCAATGGC AATCTCCACT GATCATGGAA AAGATCTCCC GACAGTCCAG
    CTTCTTATCA AAAAGAATCA GACACTCCAA AAGGAAATTT TGGGACATCA ACCACGGATT
    GATGACATCC TGGAGAGAGG CAAATCTATG ATCCAAGACA GCTCTCTGGA CCCTCAAGAG
    ATAGAAGAAA GGCTTGGGCT ACTGAAAAGC ACATGGGAAG AGCTGGCAGC AGAAGGAGGA
    AAGCGCCATG CCCGTTTAGA GGAATCTCTT GCTGCTCAGC AGTTTTATGC TGATGCGGCA
    GAAGCTGAGG CCTGGATGGG AGAGCAAGAG TTACATATGA TGTCTGAAGA AAAAGCCAAG
    GATGAGATTG GTGCCCAGGC AATGCTGAAG AAACACTTAG TAATGGAACA GGCTCTTGAT
    GACTATGCAG ACAACATCCA TCAGCTTGCC AATTCAAGCC GCGACATGGT CTCCAGAGGT
    CATCCAGAAA GTGATCGTAT TACCCTTCGT CAAGGGCAAG TGGACAAGCT ATATGCCAGT
    CTTAAAGACC TGGCAGAGGA GAGACAAGGA AAGCTACAAG AGCACCACAG ACTTTGCCAA
    CTCAAGAGAG ATGTCGAAGA CCTTGAGCAC TGGATCTCTG AACGTGAGGT GGTTGCAGCT
    TCTCATGAAC TTGGGCAAGA TTATGAGCAT GTAACGATGC TGAGAGACAA ATTCCGTGAG
    TTCTCCCGTG ACACCAGTAA CATTGGTCAA GAGCGGGTGG ATGCTGTCAA TCTGATGGCA
    GATGATTTAA TTTCATCTGG ACATTCGGAA AATGCTACTG TAGCAGATTG GAAAGACCAG
    CTAAATGAGG CATGGGCTGA TCTTCTTGAG ATGATTGACA CTCGAAGTCA GATGCTGGCA
    GCCTCATATG AACTTCACCG TTTCTACCAG GATGCAAAAG AAACCCTAGG TCAGATCCAA
    GACAAGCAGA AACAAATACC AGAGGAGACA GGCAGAGATC TGAATACAGC AGAAGCCATG
    CAGCGCATGC ACACCGCTTT TGAACATGAT ATCCAAGCCC TGAGTGCACA GGTGAAGCAA
    GTCCAGGAAG ATGCTTCTCG CCTCCAAAAA GCTTATGCTG GTGAAAAGGC AGCAGACATT
    CATCGCCATG AGCAGGCAGT ATCGGAAGCT TGGGGAGAGT TGCAGAATGC TAGTGGTGCC
    AGGCGGCAGC TTCTTCTTGA CACTGTGGAG AAATTCCGTT TCTTCAGAAT GGTGCGAGAT
    CTCATGTTAT GGATGGATGA TATAAACCTG CTAATTGATG CACAGGAGAA GCCAAGAGAT
    GTATCCTCAG CTGATTTAGT CATCCAAAAC CACCTAGGCA TCAAGGCAGA AGTGGATGCG
    CGAGCTGACA GCTTTACTAC ATGCATAGAT CTTGGCAACT CTCTCCTAGA GAAGAATCAT
    TATGCCTCTG ACAAGATTTC AGAGAAACTG GAGCAATTGC AGGAAAGAAG GCAGGAGACT
    GAAAACAAAT GGAAAGATAA AATGGCATGG CTGAGAGTTG TCCACGAAGT GCTGATGTTT
    GGAAGAGATG CAAGTGTAGC TGAGGCTTGG CTGGCCAGCC AGGAGCCCAT TGTCCGGTCG
    TCAGAACTTG GTGGCAATGT CGATGAAGTA GAATCCCTGA TAAAAAGACA CGAAGCCTTT
    CAGAAAGCAG TTTCTACATG GGATGAGAGA TTTGTTCTTC TTGAAAAACT AACCACGTTA
    GAAGAAATTG AAATGCGTCG GAAGCGGGAA GAGGAAGAAA GAAAGAAAAG ACCACCAACA
    CCACCTCTAC CAGTAGAAGA GCCTCGAGAG GAAGTTGTAA AAACCCAAGT TTCCCAGACT
    ATTGAAGGGC AGACTGATCC AGCCAATATA CTTCAAGAAA GGATAAATGG CATGCAGAAT
    TCATTCCAGG AGCAAGTATC TGTTAATGGA CTGTGCACAG AGTCTGATTC TCCACAGCCA
    TCGTCCGATA CACAGAGGTT AGAAGACAGC ATCTCTGAAA ATCAGGGTCC GGAACAGTCA
    GATGTAACCA ATGGAGCCAG GACTGAGGTT ATTACCAAAG ATGGAAGTCC AACACCTTCT
    CCACAACTTG TGGCTAGGCC AACCTTCTCC CCGATAAACC TCCAGTATTC TACTTTGCCA
    TCTAGCCCCA CTGAACCATT GGCATCTGAG CAAATGGAAG GAATGCTGCA CCGCAAGCAG
    GAAATGGATT CTCACAACAA GAAGGCTGCG AACAGGTCCT GGCAGAATGT TTACTGTGCA
    CTGAAAAGGG GCAGTCTGGG TTTCTACAAG GATGCTAAAA GTGCAGCCAG TGGCGTTTCT
    TATCATTCGG AGCCCTCCGT CCAACTTAAT GGAGCCCAGT GCAGTGTTGC TCATGACTAC
    AAGAAACGCA AGAATGTCTT CAAGCTAAGC CTTCTTGATG GCAAAGAATA TCTGTTCCAA
    GCTAAGGATG AGGCTGAAAT GAGTAGTTGG ATGCGAGTTA TAAACGCCTC CCTAACTTCC
    TCCCCGGGAG ACCCCTCAGA CCATTCACCG GGCATCATGA AGGGTATGAC CCGGGCCATG
    ACAATGCCAC CAGTATCACC CAACAGTGGT ACAGAGGGAT CAGTCACATT GCGCAACAAG
    GACGGGAAAG AAAAAGACCG GGAAAAGAGA TTCAGTTTCT TTAAGAAGAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa09015
    Clone ID Related Accession (Same CDS sequence) XM_002940505.4
    Accession Version XM_002940505.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin beta chain, non-erythrocytic 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940505.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTTTCAGT TCGACTCCCC TGCATACGAG GATGTGCCCC GGCCTGCCTC CCCCGGTCCC 
    CGGCTTGCCT CCCCTGTCCC CCGATCTGGC TCCCCTCTCC TATCCCCTGC GGCTTTTGTA
    AATGCAGTGC AATACGCTAA CGTGCTGGAA GGAAGGTTCA AGCAGCTGCA AGATGAAAGA
    GAAGCTGTGC AGAAGAAGAC ATTTACTAAA TGGGTCAACT CTCACTTGGC GAGGGTCACC
    TGTAGAATTT CTGATCTCTA CAATGATTTA CGAGATGGAC GGATGTTAAT TCGACTGTTA
    GAAGTGCTAT CTGGAGAGCA GCTGCCAAAA CCCACAAAAG GTCGAATGCG AATCCACTGT
    TTGGAAAATG TAGACAAAGC CCTTCAATTT TTAAAAGAGC AGAAGGTACA TTTGGAGAAC
    ATGGGATCAC ATGACATTGT GGATGGAAAC CATCGATTAA CCCTAGGGTT AATCTGGACT
    ATTATTTTAA GGTTTCAGAT CCAAGATATC AGTGTAGAGA CAGAAGATAA TAAAGAAAAG
    AAGTCTGCTA AAGATGCCTT ACTGCTTTGG TGCCAGATGA AGACTGCTGG GTATCCAAAT
    GTCAATGTTC ACAACTTTAC CACCAGCTGG CGAGATGGAC TTGCGTTTAA TGCCATTGTA
    CACAAGCACA GACCAGATAT AATTGACTTT GACACACTGA AGAAGTCTAA TGCACACCAC
    AACCTCCAGA ATGCCTTCAA TGTAGCTGAA AGAGAGTTAG GTCTTACTAA ACTTCTGGAT
    CCTGAAGATG TTAATGTGGA TCAGCCTGAT GAGAAATCCA TCATAACCTA TGTGGCCACA
    TTTTATCACT ATTTTTCCAA AATGAAAGCC CTAGCTGTGG AAGGAAAGCG TATTGGAAAG
    GTACTTGACC AAGCTATTGA AGCTGATCAG TTAATTGAGA ACTATGAAGG ACTGGCATCA
    GAACTCCTGC AATGGATTGA ACAGATGATC ATTACTCTCA ATGACCGAAA ACTGGCAAAC
    TCTCTAAGTG GTGTTCAGAA CCAGCTGCAG GCCTTCAACA CCTACAGGAC TGTAGAAAAG
    CCGCCCAAGT TCACAGAAAA GGGCAATTTG GAAGTTCTTC TCTTTACCAT ACAGAGTAAA
    ATGAGAGCTA ATAATCAAAA AGTGTATACG CCCAGAGAAG GAAAGCTTAT ATCTGATATA
    AACAAGGCAT GGGAGCGACT AGAAAAGGCA GAGCATGAAC GTGAGCTTGC CCTTCGCAAT
    GAACTTATAC GCCAGGAAAA ATTAGAGCAG TTGGCTGCAC GATTTGACCG TAAAGCAGCC
    ATGAGGGAAA CCTGGCTAAG TGAAAATCAG AGACTTGTAG CTCAGGATAA CTTCGGTGTG
    GACTTAACTG CGGTGGAGGC AGCGCTACGG AAACATGAAG CTATTGAAAC TGATATATTG
    GCTTACAGTG AGCGCGTGCA AGCAGTGAAT GCAGTGGCTG ATGAGCTGGA AGCAGAGAAT
    TACCATGATG TCCGGAGGAT TTTGGCTCGG AAGTGCAATG TGATTCGTCT CTCTGATTAT
    TTAAGAGAGC TTGTCACTGC CAGGAGGCAA AGGCTTCATA TGAACCTAGA ATTGCAGAGG
    ATGTTCCAGG ATTTACATTA TCTCATGGAC TGGACTGAAG ATATGAAGGG CCGTCTGCAG
    TCAGAAGATT ATGGCAAGCA TCTTCATGGC GTGGAAGACC TTCTCCAGAC TCACACTCTT
    GTGGAGGCAG ACATTGCAGT TCAGGCAGAA CGTGTGAAAT CAGTTACAGC TGTAGCTCAG
    AAATTTATGA CAGAAGATGA AGGCTACAAG CCTTGCGACC CAGAGCTAGT GCGCAACCGG
    GTGCATCTCT TGGAACAGCG CTATGCAGAG CTGTGTAATC TTTCTGCGGC TCGGAGATCA
    AAGCTGGAAG AATCCCGCCG TCTCTGGAAA TTTTTCTGGG ACATGGGAGA GGAGGAAGCC
    TGGATCCGTG AGCAGAGTAG ATTACTACTT TCTGATGACT ATGGGAAAGA CCTTACCAGC
    TCACTTAGGT TGATCAGCAA GCATAATGCC TTCAGAGGTG AGATGAGTGG ACACCGTGGG
    CCGTTGCAGC AGACTGTGGC TGAGGGTGAG GAACTAATAT CCAAAGGTCA CTTTGGTGCA
    GGAGAGATTG AGGAAAGGAT AAAAGAAGTG AAAGGCCAGT GGACAGAGAT GGAGGCCTTA
    GCTGAAGACA GGGAAAGGAG GCTAAGAGAG GCCTACAGCT ATTATCAGTT TCAAGCTGAT
    GCCAATGATG CTGAGGCTTG GATCCTGGAC ACTTTACGCC TTGTTTCTAC CAGTGAAGTA
    GGACATGATG AGTATTCCAC ACAGACACTG GTGAAAAAGC ACAAAGAAAT AGAAGAGGAG
    ATCCTTAGCC ACCGACCAGC TATTGATGCG CTGCATGAAC AGGCCCGTGG CCTTCCACCT
    GCTTACTCTC ATTCACCTGA AGTTGATGGA AGGTTACCAG CCCTGGAGCA GCGCTATGAG
    GAATTGGTGG CTCTTGCTGC TCACCGCAAG CAGGCTCTGC AAGATTCTCT GGCTCTTCAT
    AAAATGTTTA GTGAAGCTGA TGCTTGCCTT TTGTGGATTG ATGAGAAAGA ACAGTGGCTT
    AATGTCACAG AGATTCCAGA AAAACTTGAG GACCTAGAAG TAGTTCAACA AAGGTTTGAA
    ACTCTAGAGG CAGAGATGAA TAATTTGGCA TCCCGGATTG CATCTGTAAA TGAAGTTGCT
    GGACAGTTGC TGACTACAGA CCAGAGAAAT GAAAACAGCA TCCGAGACAT GCAAGATCAA
    CTTAATTCTC GGTGGAATGA ATTCCGCAAG CTTGTTGACC AAAAGAAGGG TGCTTTGGAT
    TCTGCTCTGG ATATACAGAA TTATCATTTG GAGTGCAATG AGACTGTGTG CTGGATGAGG
    GAGAAGACAA AGGTTATTGA ATCCACACAG AGCCTAGGGA ATGATCTTGC AGGAGTAACA
    GCCCTCCAGA GAAAACTTAC TGGAATGGAG CGTGATCTGA AAGCCATTCA GGGAAAAGTG
    GGTGACCTGC GTTCAGAGGC AGAAAAGCTT GCAGATGGGC ACCCACAGAA AGCACCGGCC
    ATAATTGGCC GCTTATCAGA TATTGATGAG ACCTGGGGAG AACTAAAGGA CACTATGAAA
    AGAAGAGAAG AAACTTTAGG TGAAGCAAGC AAGCTGCAGG GATTTTTGAG AGACCTTGAT
    GATTTCCAGA GCTGGTTGTC CCGGGCCCAG ACAGCAGTGG CATCTGAGGA TGTTCCAGCT
    GACCTAGCAG AAGCAGAGCG CCTTCTTGCA CAACATGAAG GCATAAAAAA TGAGATTGAT
    CATTATGGAG CTGACTACCA CAGAGTGAGA GATGTTGGTG AAGAAGTCAC TCGTGGGCAA
    ACAGATGCCC AATATATGTT CTTGCAACAG AGACTGAAGG CGCTAGACAC TGGCTGGAAT
    GAGCTTGGAC AGATGTGGGA AAACAGGCAC CAGTTGTTGT CCCAGGGTCA CAGCTTCCAG
    CAGTTTTTGA AAGATACAAA ACAAGTGGAG GGGTTCCTTA ATAACCAGGA GTATGTCCTT
    TCCCATACCG AAATGCCAAG TACGCTACAG GCAGCTGAAG CTGCCATCAA ACGCCATGAA
    GATTTCCTCA CAACCATGGA TGCCAATGAA GAGAAGATAC AGGGTCTTGT AGAATCTGGG
    AGGAAGCTAA TTGAGGAACA GAATGTCAAT TCTGAGAAGA TTCAAGAAAA AGTCACTTCT
    ATTGATGAGA GACACAAAAA GAACCAAAAA ACTGCTAGCG AAGTATTAAC TCGTTTGAAG
    GACAACCGTG ATCTTCAACA TTTCCTTCAA GACTGTCAAG AGCTCACCCT CTGGATCAAT
    GAAAAAATGC TTACTGCTCA AGATGTTTCT TATGATGAAG CTCGAAACCT GCATAGCAAG
    TGGCAAAAGC ATCAGGCATT CATGGCAGAA CTGGCCTCAA ATAAAGACTG GCTTGACAAA
    ATAGACAAGG AAGGGCAGCA GCTCATGTCA GAAAAACCGG AGCTTGAACC AGTAGTGAGA
    GAAAAACTAG ACACTCTGCA TAAGCTGTGG AATGACTTGG AATCTACCAC ACAAAGTAAA
    GCTCAGTGTC TGTTTGATGC CAACAGGGCA GAGTTATTCA CACAAAGCTG TTCTGCTCTG
    GAGAGTTGGC TTCAAAACCT CGAGTCACAA CTACAGTCAG ATCATTATGG CAAAGATCTG
    ACCAGTGTCA ACATCCTATT AAATAAACAG CAGATGCTGG AGAACCAAAT GGAAGTGAGG
    GAGAAGGAGC TAGAAGCTAT ACAGTCCCAG GCACAGGCCC TTGGACAGGA GGATGCCAAC
    ATAGTGGAGG TTGACTCAAA GCATAAGGAT GTAGCAGATA AATTTTCCAA GCTGCGTGCT
    CCTCTAAACA AGAGAAGAAA ATTACTTCTA GCCTCCAAGG AAGGACATCA GTTTAACAGG
    GACCTGGAAG ATGAAATACT TTGGGTAAAG GAGAAGATGC CAATGGCAAT CTCCACTGAT
    CATGGAAAAG ATCTCCCGAC AGTCCAGCTT CTTATCAAAA AGAATCAGAC ACTCCAAAAG
    GAAATTTTGG GACATCAACC ACGGATTGAT GACATCCTGG AGAGAGGCAA ATCTATGATC
    CAAGACAGCT CTCTGGACCC TCAAGAGATA GAAGAAAGGC TTGGGCTACT GAAAAGCACA
    TGGGAAGAGC TGGCAGCAGA AGGAGGAAAG CGCCATGCCC GTTTAGAGGA ATCTCTTGCT
    GCTCAGCAGT TTTATGCTGA TGCGGCAGAA GCTGAGGCCT GGATGGGAGA GCAAGAGTTA
    CATATGATGT CTGAAGAAAA AGCCAAGGAT GAGATTGGTG CCCAGGCTAT GCTGAAGAAA
    CACTTAGTAA TGGAACAGGC TCTTGATGAC TATGCAGACA ACATCCATCA GCTTGCCAAT
    TCAAGCCGCG ACATGGTCTC CAGAGGTCAT CCAGAAAGTG ATCGTATTAC CCTTCGTCAA
    GGGCAAGTGG ACAAGCTATA TGCCAGTCTT AAAGACCTGG CAGAGGAGAG ACAAGGAAAG
    CTACAAGAGC ACCACAGACT TTGCCAACTC AAGAGAGATG TCGAAGACCT TGAGCACTGG
    ATCTCTGAAC GTGAGGTGGT TGCAGCTTCT CATGAACTTG GGCAAGATTA TGAGCATGTA
    ACGATGCTGA GAGACAAATT CCGTGAGTTC TCCCGTGACA CCAGTAACAT TGGTCAAGAG
    CGGGTGGATG CTGTCAATCT GATGGCAGAT GATTTAATTT CATCTGGACA TTCGGAAAAT
    GCTACTGTAG CAGATTGGAA AGACCAGCTA AATGAGGCAT GGGCTGATCT TCTTGAGATG
    ATCGACACTC GAAGTCAGAT GCTGGCAGCC TCATATGAAC TTCACCGTTT CTACCAGGAT
    GCAAAAGAAA CCCTAGGTCA GATCCAAGAC AAGCAGAAAC AAATACCAGA GGAGACAGGC
    AGAGATCTGA ATACAGCAGA AGCCATGCAG CGCATGCACA CCGCTTTTGA ACATGATATC
    CAAGCCCTGA GTGCACAGGT GAAGCAAGTC CAGGAAGATG CTTCTCGCCT CCAAAAAGCT
    TATGCTGGTG AAAAGGCAGC AGACATTCAT CGCCATGAGC AGGCAGTATC GGAAGCTTGG
    GGAGAGTTGC AGAATGCTAG TGGTGCCAGG CGGCAGCTTC TTCTTGACAC TGTGGAGAAA
    TTCCGTTTCT TCAGAATGGT GCGAGATCTC ATGTTATGGA TGGATGATAT AAACCTGCTA
    ATTGATGCAC AGGAGAAGCC AAGAGATGTA TCCTCAGCTG ATTTAGTCAT CCAAAACCAC
    CTAGGCATCA AGGCAGAAGT GGATGCGCGA GCTGACAGCT TTACTACATG CATAGATCTT
    GGCAACTCTC TCCTAGAGAA GAATCATTAT GCCTCTGACA AGATTTCAGA GAAACTGGAG
    CAATTGCAGG AAAGAAGGCA GGAGACTGAA AACAAATGGA AAGATAAAAT GGCATGGCTG
    AGAGTTGTCC ACGAAGTGCT GATGTTTGGA AGAGATGCAA GTGTAGCTGA GGCTTGGCTG
    GCCAGCCAGG AGCCCATTGT CCGGTCGTCA GAACTTGGTG GCAATGTCGA TGAAGTAGAA
    TCCCTGATAA AAAGACACGA AGCCTTTCAG AAAGCAGTTT CTACATGGGA TGAGAGATTT
    GTTCTTCTTG AAAAACTAAC CACGTTAGAA GAAATTGAAA TGCGTCGGAA GCGGGAAGAG
    GAAGAAAGAA AGAAAAGACC ACCAACACCA CCTCTACCAG TAGAAGAGCC TCGAGAGGAA
    GTTGTAAAAA CCCAAGTTTC CCAGACTATT GAAGGACAGA CTGATCCAGC CAATATACTT
    CAAGAAAGGA TAAATGGCAT GCAGAATTCA TTCCAGGAGC AAGTATCTGT TAATGGACTG
    TGCACAGAGT CTGATTCTCC ACAGCCATCG TCCGATACAC AGAGGTTAGA AGACAGCATC
    TCTGAAAATC AGGGTCCGGA ACAGTCAGAT GTAACCAATG GAGCCAGGAC TGAGGTTATT
    ACCAAAGATG GAAGTCCAAC ACCTTCTCCA CAACTTGTGG CTAGGCCAAC CTTCTCCCCG
    ATAAACCTCC AGTATTCTAC TTTGCCATCT AGCCCCACTG AACCATTGGC ATCTGAGCAA
    ATGGAAGGAA TGCTGCACCG CAAGCAGGAA ATGGATTCTC ACAACAAGAA GGCTGCGAAC
    AGGTCCTGGC AGAATGTTTA CTGTGCACTG AAAAGGGGCA GTCTGGGTTT CTACAAGGAT
    GCTAAAAGTG CAGCCAGTGG CGTTTCTTAT CATTCGGAGC CCTCCGTCCA ACTTAATGGA
    GCCCAGTGCA GTGTTGCTCA TGACTACAAG AAACGCAAGA ATGTCTTCAA GCTAAGCCTT
    CTTGATGGCA AAGAATATCT GTTCCAAGCT AAGGATGAGG CTGAAATGAG TAGTTGGATG
    CGAGTTATAA ACGCCTCCCT AACTTCCTCC CCGGGAGACC CCTCAGACCA TTCACCGGGC
    ATCATGAAGG GTATGACCCG GGCCATGACA ATGCCACCAG TATCACCCAA CAGTGGTACA
    GAGGGATCAG TCACATTGCG CAACAAGGAC GGGAAAGAAA AAGACCGGGA AAAGAGATTC
    AGTTTCTTTA AGAAGAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002940551.2
    CDS908..8128
    Translation

    Target ORF information:

    RefSeq Version XM_002940505.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002940505.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTTTCAGT TCGACTCCCC TGCATACGAG GATGTGCCCC GGCCTGCCTC CCCCGGTCCC 
    CGGCTTGCCT CCCCTGTCCC CCGATCTGGC TCCCCTCTCC TATCCCCTGC GGCTTTTGTA
    AATGCAGTGC AATACGCTAA CGTGCTGGAA GGAAGGTTCA AGCAGCTGCA AGATGAAAGA
    GAAGCTGTGC AGAAGAAGAC ATTTACTAAA TGGGTCAACT CTCACTTGGC GAGGGTCACC
    TGTAGAATTT CTGATCTCTA CAATGATTTA CGAGATGGAC GGATGTTAAT TCGACTGTTA
    GAAGTGCTAT CTGGAGAGCA GCTGCCAAAA CCCACAAAAG GTCGAATGCG AATCCACTGT
    TTGGAAAATG TAGACAAAGC CCTTCAATTT TTAAAAGAGC AGAAGGTACA TTTGGAGAAC
    ATGGGATCAC ATGACATTGT GGATGGAAAC CATCGATTAA CCCTAGGGTT AATCTGGACT
    ATTATTTTAA GGTTTCAGAT CCAAGATATC AGTGTAGAGA CAGAAGATAA TAAAGAAAAG
    AAGTCTGCTA AAGATGCCTT ACTGCTTTGG TGCCAGATGA AGACTGCTGG GTATCCAAAT
    GTCAATGTTC ACAACTTTAC CACCAGCTGG CGAGATGGAC TTGCGTTTAA TGCCATTGTA
    CACAAGCACA GACCAGATAT AATTGACTTT GACACACTGA AGAAGTCTAA TGCACACCAC
    AACCTCCAGA ATGCCTTCAA TGTAGCTGAA AGAGAGTTAG GTCTTACTAA ACTTCTGGAT
    CCTGAAGATG TTAATGTGGA TCAGCCTGAT GAGAAATCCA TCATAACCTA TGTGGCCACA
    TTTTATCACT ATTTTTCCAA AATGAAAGCC CTAGCTGTGG AAGGAAAGCG TATTGGAAAG
    GTACTTGACC AAGCTATTGA AGCTGATCAG TTAATTGAGA ACTATGAAGG ACTGGCATCA
    GAACTCCTGC AATGGATTGA ACAGATGATC ATTACTCTCA ATGACCGAAA ACTGGCAAAC
    TCTCTAAGTG GTGTTCAGAA CCAGCTGCAG GCCTTCAACA CCTACAGGAC TGTAGAAAAG
    CCGCCCAAGT TCACAGAAAA GGGCAATTTG GAAGTTCTTC TCTTTACCAT ACAGAGTAAA
    ATGAGAGCTA ATAATCAAAA AGTGTATACG CCCAGAGAAG GAAAGCTTAT ATCTGATATA
    AACAAGGCAT GGGAGCGACT AGAAAAGGCA GAGCATGAAC GTGAGCTTGC CCTTCGCAAT
    GAACTTATAC GCCAGGAAAA ATTAGAGCAG TTGGCTGCAC GATTTGACCG TAAAGCAGCC
    ATGAGGGAAA CCTGGCTAAG TGAAAATCAG AGACTTGTAG CTCAGGATAA CTTCGGTGTG
    GACTTAACTG CGGTGGAGGC AGCGCTACGG AAACATGAAG CTATTGAAAC TGATATATTG
    GCTTACAGTG AGCGCGTGCA AGCAGTGAAT GCAGTGGCTG ATGAGCTGGA AGCAGAGAAT
    TACCATGATG TCCGGAGGAT TTTGGCTCGG AAGTGCAATG TGATTCGTCT CTCTGATTAT
    TTAAGAGAGC TTGTCACTGC CAGGAGGCAA AGGCTTCATA TGAACCTAGA ATTGCAGAGG
    ATGTTCCAGG ATTTACATTA TCTCATGGAC TGGACTGAAG ATATGAAGGG CCGTCTGCAG
    TCAGAAGATT ATGGCAAGCA TCTTCATGGC GTGGAAGACC TTCTCCAGAC TCACACTCTT
    GTGGAGGCAG ACATTGCAGT TCAGGCAGAA CGTGTGAAAT CAGTTACAGC TGTAGCTCAG
    AAATTTATGA CAGAAGATGA AGGCTACAAG CCTTGCGACC CAGAGCTAGT GCGCAACCGG
    GTGCATCTCT TGGAACAGCG CTATGCAGAG CTGTGTAATC TTTCTGCGGC TCGGAGATCA
    AAGCTGGAAG AATCCCGCCG TCTCTGGAAA TTTTTCTGGG ACATGGGAGA GGAGGAAGCC
    TGGATCCGTG AGCAGAGTAG ATTACTACTT TCTGATGACT ATGGGAAAGA CCTTACCAGC
    TCACTTAGGT TGATCAGCAA GCATAATGCC TTCAGAGGTG AGATGAGTGG ACACCGTGGG
    CCGTTGCAGC AGACTGTGGC TGAGGGTGAG GAACTAATAT CCAAAGGTCA CTTTGGTGCA
    GGAGAGATTG AGGAAAGGAT AAAAGAAGTG AAAGGCCAGT GGACAGAGAT GGAGGCCTTA
    GCTGAAGACA GGGAAAGGAG GCTAAGAGAG GCCTACAGCT ATTATCAGTT TCAAGCTGAT
    GCCAATGATG CTGAGGCTTG GATCCTGGAC ACTTTACGCC TTGTTTCTAC CAGTGAAGTA
    GGACATGATG AGTATTCCAC ACAGACACTG GTGAAAAAGC ACAAAGAAAT AGAAGAGGAG
    ATCCTTAGCC ACCGACCAGC TATTGATGCG CTGCATGAAC AGGCCCGTGG CCTTCCACCT
    GCTTACTCTC ATTCACCTGA AGTTGATGGA AGGTTACCAG CCCTGGAGCA GCGCTATGAG
    GAATTGGTGG CTCTTGCTGC TCACCGCAAG CAGGCTCTGC AAGATTCTCT GGCTCTTCAT
    AAAATGTTTA GTGAAGCTGA TGCTTGCCTT TTGTGGATTG ATGAGAAAGA ACAGTGGCTT
    AATGTCACAG AGATTCCAGA AAAACTTGAG GACCTAGAAG TAGTTCAACA AAGGTTTGAA
    ACTCTAGAGG CAGAGATGAA TAATTTGGCA TCCCGGATTG CATCTGTAAA TGAAGTTGCT
    GGACAGTTGC TGACTACAGA CCAGAGAAAT GAAAACAGCA TCCGAGACAT GCAAGATCAA
    CTTAATTCTC GGTGGAATGA ATTCCGCAAG CTTGTTGACC AAAAGAAGGG TGCTTTGGAT
    TCTGCTCTGG ATATACAGAA TTATCATTTG GAGTGCAATG AGACTGTGTG CTGGATGAGG
    GAGAAGACAA AGGTTATTGA ATCCACACAG AGCCTAGGGA ATGATCTTGC AGGAGTAACA
    GCCCTCCAGA GAAAACTTAC TGGAATGGAG CGTGATCTGA AAGCCATTCA GGGAAAAGTG
    GGTGACCTGC GTTCAGAGGC AGAAAAGCTT GCAGATGGGC ACCCACAGAA AGCACCGGCC
    ATAATTGGCC GCTTATCAGA TATTGATGAG ACCTGGGGAG AACTAAAGGA CACTATGAAA
    AGAAGAGAAG AAACTTTAGG TGAAGCAAGC AAGCTGCAGG GATTTTTGAG AGACCTTGAT
    GATTTCCAGA GCTGGTTGTC CCGGGCCCAG ACAGCAGTGG CATCTGAGGA TGTTCCAGCT
    GACCTAGCAG AAGCAGAGCG CCTTCTTGCA CAACATGAAG GCATAAAAAA TGAGATTGAT
    CATTATGGAG CTGACTACCA CAGAGTGAGA GATGTTGGTG AAGAAGTCAC TCGTGGGCAA
    ACAGATGCCC AATATATGTT CTTGCAACAG AGACTGAAGG CGCTAGACAC TGGCTGGAAT
    GAGCTTGGAC AGATGTGGGA AAACAGGCAC CAGTTGTTGT CCCAGGGTCA CAGCTTCCAG
    CAGTTTTTGA AAGATACAAA ACAAGTGGAG GGGTTCCTTA ATAACCAGGA GTATGTCCTT
    TCCCATACCG AAATGCCAAG TACGCTACAG GCAGCTGAAG CTGCCATCAA ACGCCATGAA
    GATTTCCTCA CAACCATGGA TGCCAATGAA GAGAAGATAC AGGGTCTTGT AGAATCTGGG
    AGGAAGCTAA TTGAGGAACA GAATGTCAAT TCTGAGAAGA TTCAAGAAAA AGTCACTTCT
    ATTGATGAGA GACACAAAAA GAACCAAAAA ACTGCTAGCG AAGTATTAAC TCGTTTGAAG
    GACAACCGTG ATCTTCAACA TTTCCTTCAA GACTGTCAAG AGCTCACCCT CTGGATCAAT
    GAAAAAATGC TTACTGCTCA AGATGTTTCT TATGATGAAG CTCGAAACCT GCATAGCAAG
    TGGCAAAAGC ATCAGGCATT CATGGCAGAA CTGGCCTCAA ATAAAGACTG GCTTGACAAA
    ATAGACAAGG AAGGGCAGCA GCTCATGTCA GAAAAACCGG AGCTTGAACC AGTAGTGAGA
    GAAAAACTAG ACACTCTGCA TAAGCTGTGG AATGACTTGG AATCTACCAC ACAAAGTAAA
    GCTCAGTGTC TGTTTGATGC CAACAGGGCA GAGTTATTCA CACAAAGCTG TTCTGCTCTG
    GAGAGTTGGC TTCAAAACCT CGAGTCACAA CTACAGTCAG ATCATTATGG CAAAGATCTG
    ACCAGTGTCA ACATCCTATT AAATAAACAG CAGATGCTGG AGAACCAAAT GGAAGTGAGG
    GAGAAGGAGC TAGAAGCTAT ACAGTCCCAG GCACAGGCCC TTGGACAGGA GGATGCCAAC
    ATAGTGGAGG TTGACTCAAA GCATAAGGAT GTAGCAGATA AATTTTCCAA GCTGCGTGCT
    CCTCTAAACA AGAGAAGAAA ATTACTTCTA GCCTCCAAGG AAGGACATCA GTTTAACAGG
    GACCTGGAAG ATGAAATACT TTGGGTAAAG GAGAAGATGC CAATGGCAAT CTCCACTGAT
    CATGGAAAAG ATCTCCCGAC AGTCCAGCTT CTTATCAAAA AGAATCAGAC ACTCCAAAAG
    GAAATTTTGG GACATCAACC ACGGATTGAT GACATCCTGG AGAGAGGCAA ATCTATGATC
    CAAGACAGCT CTCTGGACCC TCAAGAGATA GAAGAAAGGC TTGGGCTACT GAAAAGCACA
    TGGGAAGAGC TGGCAGCAGA AGGAGGAAAG CGCCATGCCC GTTTAGAGGA ATCTCTTGCT
    GCTCAGCAGT TTTATGCTGA TGCGGCAGAA GCTGAGGCCT GGATGGGAGA GCAAGAGTTA
    CATATGATGT CTGAAGAAAA AGCCAAGGAT GAGATTGGTG CCCAGGCTAT GCTGAAGAAA
    CACTTAGTAA TGGAACAGGC TCTTGATGAC TATGCAGACA ACATCCATCA GCTTGCCAAT
    TCAAGCCGCG ACATGGTCTC CAGAGGTCAT CCAGAAAGTG ATCGTATTAC CCTTCGTCAA
    GGGCAAGTGG ACAAGCTATA TGCCAGTCTT AAAGACCTGG CAGAGGAGAG ACAAGGAAAG
    CTACAAGAGC ACCACAGACT TTGCCAACTC AAGAGAGATG TCGAAGACCT TGAGCACTGG
    ATCTCTGAAC GTGAGGTGGT TGCAGCTTCT CATGAACTTG GGCAAGATTA TGAGCATGTA
    ACGATGCTGA GAGACAAATT CCGTGAGTTC TCCCGTGACA CCAGTAACAT TGGTCAAGAG
    CGGGTGGATG CTGTCAATCT GATGGCAGAT GATTTAATTT CATCTGGACA TTCGGAAAAT
    GCTACTGTAG CAGATTGGAA AGACCAGCTA AATGAGGCAT GGGCTGATCT TCTTGAGATG
    ATCGACACTC GAAGTCAGAT GCTGGCAGCC TCATATGAAC TTCACCGTTT CTACCAGGAT
    GCAAAAGAAA CCCTAGGTCA GATCCAAGAC AAGCAGAAAC AAATACCAGA GGAGACAGGC
    AGAGATCTGA ATACAGCAGA AGCCATGCAG CGCATGCACA CCGCTTTTGA ACATGATATC
    CAAGCCCTGA GTGCACAGGT GAAGCAAGTC CAGGAAGATG CTTCTCGCCT CCAAAAAGCT
    TATGCTGGTG AAAAGGCAGC AGACATTCAT CGCCATGAGC AGGCAGTATC GGAAGCTTGG
    GGAGAGTTGC AGAATGCTAG TGGTGCCAGG CGGCAGCTTC TTCTTGACAC TGTGGAGAAA
    TTCCGTTTCT TCAGAATGGT GCGAGATCTC ATGTTATGGA TGGATGATAT AAACCTGCTA
    ATTGATGCAC AGGAGAAGCC AAGAGATGTA TCCTCAGCTG ATTTAGTCAT CCAAAACCAC
    CTAGGCATCA AGGCAGAAGT GGATGCGCGA GCTGACAGCT TTACTACATG CATAGATCTT
    GGCAACTCTC TCCTAGAGAA GAATCATTAT GCCTCTGACA AGATTTCAGA GAAACTGGAG
    CAATTGCAGG AAAGAAGGCA GGAGACTGAA AACAAATGGA AAGATAAAAT GGCATGGCTG
    AGAGTTGTCC ACGAAGTGCT GATGTTTGGA AGAGATGCAA GTGTAGCTGA GGCTTGGCTG
    GCCAGCCAGG AGCCCATTGT CCGGTCGTCA GAACTTGGTG GCAATGTCGA TGAAGTAGAA
    TCCCTGATAA AAAGACACGA AGCCTTTCAG AAAGCAGTTT CTACATGGGA TGAGAGATTT
    GTTCTTCTTG AAAAACTAAC CACGTTAGAA GAAATTGAAA TGCGTCGGAA GCGGGAAGAG
    GAAGAAAGAA AGAAAAGACC ACCAACACCA CCTCTACCAG TAGAAGAGCC TCGAGAGGAA
    GTTGTAAAAA CCCAAGTTTC CCAGACTATT GAAGGACAGA CTGATCCAGC CAATATACTT
    CAAGAAAGGA TAAATGGCAT GCAGAATTCA TTCCAGGAGC AAGTATCTGT TAATGGACTG
    TGCACAGAGT CTGATTCTCC ACAGCCATCG TCCGATACAC AGAGGTTAGA AGACAGCATC
    TCTGAAAATC AGGGTCCGGA ACAGTCAGAT GTAACCAATG GAGCCAGGAC TGAGGTTATT
    ACCAAAGATG GAAGTCCAAC ACCTTCTCCA CAACTTGTGG CTAGGCCAAC CTTCTCCCCG
    ATAAACCTCC AGTATTCTAC TTTGCCATCT AGCCCCACTG AACCATTGGC ATCTGAGCAA
    ATGGAAGGAA TGCTGCACCG CAAGCAGGAA ATGGATTCTC ACAACAAGAA GGCTGCGAAC
    AGGTCCTGGC AGAATGTTTA CTGTGCACTG AAAAGGGGCA GTCTGGGTTT CTACAAGGAT
    GCTAAAAGTG CAGCCAGTGG CGTTTCTTAT CATTCGGAGC CCTCCGTCCA ACTTAATGGA
    GCCCAGTGCA GTGTTGCTCA TGACTACAAG AAACGCAAGA ATGTCTTCAA GCTAAGCCTT
    CTTGATGGCA AAGAATATCT GTTCCAAGCT AAGGATGAGG CTGAAATGAG TAGTTGGATG
    CGAGTTATAA ACGCCTCCCT AACTTCCTCC CCGGGAGACC CCTCAGACCA TTCACCGGGC
    ATCATGAAGG GTATGACCCG GGCCATGACA ATGCCACCAG TATCACCCAA CAGTGGTACA
    GAGGGATCAG TCACATTGCG CAACAAGGAC GGGAAAGAAA AAGACCGGGA AAAGAGATTC
    AGTTTCTTTA AGAAGAAGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa09016
    Clone ID Related Accession (Same CDS sequence) XM_004913695.3
    Accession Version XM_004913695.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004913695.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
    GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA
    AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGATG AAAGAGAAGC TGTGCAGAAG
    AAGACATTTA CTAAATGGGT CAACTCTCAC TTGGCGAGGG TCACCTGTAG AATTTCTGAT
    CTCTACAATG ATTTACGAGA TGGACGGATG TTAATTCGAC TGTTAGAAGT GCTATCTGGA
    GAGCAGCTGC CAAAACCCAC AAAAGGTCGA ATGCGAATCC ACTGTTTGGA AAATGTAGAC
    AAAGCCCTTC AATTTTTAAA AGAGCAGAAG GTACATTTGG AGAACATGGG ATCACATGAC
    ATTGTGGATG GAAACCATCG ATTAACCCTA GGGTTAATCT GGACTATTAT TTTAAGGTTT
    CAGATCCAAG ATATCAGTGT AGAGACAGAA GATAATAAAG AAAAGAAGTC TGCTAAAGAT
    GCCTTACTGC TTTGGTGCCA GATGAAGACT GCTGGGTATC CAAATGTCAA TGTTCACAAC
    TTTACCACCA GCTGGCGAGA TGGACTTGCG TTTAATGCCA TTGTACACAA GCACAGACCA
    GATATAATTG ACTTTGACAC ACTGAAGAAG TCTAATGCAC ACCACAACCT CCAGAATGCC
    TTCAATGTAG CTGAAAGAGA GTTAGGTCTT ACTAAACTTC TGGATCCTGA AGATGTTAAT
    GTGGATCAGC CTGATGAGAA ATCCATCATA ACCTATGTGG CCACATTTTA TCACTATTTT
    TCCAAAATGA AAGCCCTAGC TGTGGAAGGA AAGCGTATTG GAAAGGTACT TGACCAAGCT
    ATTGAAGCTG ATCAGTTAAT TGAGAACTAT GAAGGACTGG CATCAGAACT CCTGCAATGG
    ATTGAACAGA TGATCATTAC TCTCAATGAC CGAAAACTGG CAAACTCTCT AAGTGGTGTT
    CAGAACCAGC TGCAGGCCTT CAACACCTAC AGGACTGTAG AAAAGCCGCC CAAGTTCACA
    GAAAAGGGCA ATTTGGAAGT TCTTCTCTTT ACCATACAGA GTAAAATGAG AGCTAATAAT
    CAAAAAGTGT ATACGCCCAG AGAAGGAAAG CTTATATCTG ATATAAACAA GGCATGGGAG
    CGACTAGAAA AGGCAGAGCA TGAACGTGAG CTTGCCCTTC GCAATGAACT TATACGCCAG
    GAAAAATTAG AGCAGTTGGC TGCACGATTT GACCGTAAAG CAGCCATGAG GGAAACCTGG
    CTAAGTGAAA ATCAGAGACT TGTAGCTCAG GATAACTTCG GTGTGGACTT AACTGCGGTG
    GAGGCAGCGC TACGGAAACA TGAAGCTATT GAAACTGATA TATTGGCTTA CAGTGAGCGC
    GTGCAAGCAG TGAATGCAGT GGCTGATGAG CTGGAAGCAG AGAATTACCA TGATGTCCGG
    AGGATTTTGG CTCGGAAGTG CAATGTGATT CGTCTCTCTG ATTATTTAAG AGAGCTTGTC
    ACTGCCAGGA GGCAAAGGCT TCATATGAAC CTAGAATTGC AGAGGATGTT CCAGGATTTA
    CATTATCTCA TGGACTGGAC TGAAGATATG AAGGGCCGTC TGCAGTCAGA AGATTATGGC
    AAGCATCTTC ATGGCGTGGA AGACCTTCTC CAGACTCACA CTCTTGTGGA GGCAGACATT
    GCAGTTCAGG CAGAACGTGT GAAATCAGTT ACAGCTGTAG CTCAGAAATT TATGACAGAA
    GATGAAGGCT ACAAGCCTTG CGACCCAGAG CTAGTGCGCA ACCGGGTGCA TCTCTTGGAA
    CAGCGCTATG CAGAGCTGTG TAATCTTTCT GCGGCTCGGA GATCAAAGCT GGAAGAATCC
    CGCCGTCTCT GGAAATTTTT CTGGGACATG GGAGAGGAGG AAGCCTGGAT CCGTGAGCAG
    AGTAGATTAC TACTTTCTGA TGACTATGGG AAAGACCTTA CCAGCTCACT TAGGTTGATC
    AGCAAGCATA ATGCCTTCAG AGGTGAGATG AGTGGACACC GTGGGCCGTT GCAGCAGACT
    GTGGCTGAGG GTGAGGAACT AATATCCAAA GGTCACTTTG GTGCAGGAGA GATTGAGGAA
    AGGATAAAAG AAGTGAAAGG CCAGTGGACA GAGATGGAGG CCTTAGCTGA AGACAGGGAA
    AGGAGGCTAA GAGAGGCCTA CAGCTATTAT CAGTTTCAAG CTGATGCCAA TGATGCTGAG
    GCTTGGATCC TGGACACTTT ACGCCTTGTT TCTACCAGTG AAGTAGGACA TGATGAGTAT
    TCCACACAGA CACTGGTGAA AAAGCACAAA GAAATAGAAG AGGAGATCCT TAGCCACCGA
    CCAGCTATTG ATGCGCTGCA TGAACAGGCC CGTGGCCTTC CACCTGCTTA CTCTCATTCA
    CCTGAAGTTG ATGGAAGGTT ACCAGCCCTG GAGCAGCGCT ATGAGGAATT GGTGGCTCTT
    GCTGCTCACC GCAAGCAGGC TCTGCAAGAT TCTCTGGCTC TTCATAAAAT GTTTAGTGAA
    GCTGATGCTT GCCTTTTGTG GATTGATGAG AAAGAACAGT GGCTTAATGT CACAGAGATT
    CCAGAAAAAC TTGAGGACCT AGAAGTAGTT CAACAAAGGT TTGAAACTCT AGAGGCAGAG
    ATGAATAATT TGGCATCCCG GATTGCATCT GTAAATGAAG TTGCTGGACA GTTGCTGACT
    ACAGACCAGA GAAATGAAAA CAGCATCCGA GACATGCAAG ATCAACTTAA TTCTCGGTGG
    AATGAATTCC GCAAGCTTGT TGACCAAAAG AAGGGTGCTT TGGATTCTGC TCTGGATATA
    CAGAATTATC ATTTGGAGTG CAATGAGACT GTGTGCTGGA TGAGGGAGAA GACAAAGGTT
    ATTGAATCCA CACAGAGCCT AGGGAATGAT CTTGCAGGAG TAACAGCCCT CCAGAGAAAA
    CTTACTGGAA TGGAGCGTGA TCTGAAAGCC ATTCAGGGAA AAGTGGGTGA CCTGCGTTCA
    GAGGCAGAAA AGCTTGCAGA TGGGCACCCA CAGAAAGCAC CGGCCATAAT TGGCCGCTTA
    TCAGATATTG ATGAGACCTG GGGAGAACTA AAGGACACTA TGAAAAGAAG AGAAGAAACT
    TTAGGTGAAG CAAGCAAGCT GCAGGGATTT TTGAGAGACC TTGATGATTT CCAGAGCTGG
    TTGTCCCGGG CCCAGACAGC AGTGGCATCT GAGGATGTTC CAGCTGACCT AGCAGAAGCA
    GAGCGCCTTC TTGCACAACA TGAAGGCATA AAAAATGAGA TTGATCATTA TGGAGCTGAC
    TACCACAGAG TGAGAGATGT TGGTGAAGAA GTCACTCGTG GGCAAACAGA TGCCCAATAT
    ATGTTCTTGC AACAGAGACT GAAGGCGCTA GACACTGGCT GGAATGAGCT TGGACAGATG
    TGGGAAAACA GGCACCAGTT GTTGTCCCAG GGTCACAGCT TCCAGCAGTT TTTGAAAGAT
    ACAAAACAAG TGGAGGGGTT CCTTAATAAC CAGGAGTATG TCCTTTCCCA TACCGAAATG
    CCAAGTACGC TACAGGCAGC TGAAGCTGCC ATCAAACGCC ATGAAGATTT CCTCACAACC
    ATGGATGCCA ATGAAGAGAA GATACAGGGT CTTGTAGAAT CTGGGAGGAA GCTAATTGAG
    GAACAGAATG TCAATTCTGA GAAGATTCAA GAAAAAGTCA CTTCTATTGA TGAGAGACAC
    AAAAAGAACC AAAAAACTGC TAGCGAAGTA TTAACTCGTT TGAAGGACAA CCGTGATCTT
    CAACATTTCC TTCAAGACTG TCAAGAGCTC ACCCTCTGGA TCAATGAAAA AATGCTTACT
    GCTCAAGATG TTTCTTATGA TGAAGCTCGA AACCTGCATA GCAAGTGGCA AAAGCATCAG
    GCATTCATGG CAGAACTGGC CTCAAATAAA GACTGGCTTG ACAAAATAGA CAAGGAAGGG
    CAGCAGCTCA TGTCAGAAAA ACCGGAGCTT GAACCAGTAG TGAGAGAAAA ACTAGACACT
    CTGCATAAGC TGTGGAATGA CTTGGAATCT ACCACACAAA GTAAAGCTCA GTGTCTGTTT
    GATGCCAACA GGGCAGAGTT ATTCACACAA AGCTGTTCTG CTCTGGAGAG TTGGCTTCAA
    AACCTCGAGT CACAACTACA GTCAGATCAT TATGGCAAAG ATCTGACCAG TGTCAACATC
    CTATTAAATA AACAGCAGAT GCTGGAGAAC CAAATGGAAG TGAGGGAGAA GGAGCTAGAA
    GCTATACAGT CCCAGGCACA GGCCCTTGGA CAGGAGGATG CCAACATAGT GGAGGTTGAC
    TCAAAGCATA AGGATGTAGC AGATAAATTT TCCAAGCTGC GTGCTCCTCT AAACAAGAGA
    AGAAAATTAC TTCTAGCCTC CAAGGAAGGA CATCAGTTTA ACAGGGACCT GGAAGATGAA
    ATACTTTGGG TAAAGGAGAA GATGCCAATG GCAATCTCCA CTGATCATGG AAAAGATCTC
    CCGACAGTCC AGCTTCTTAT CAAAAAGAAT CAGACACTCC AAAAGGAAAT TTTGGGACAT
    CAACCACGGA TTGATGACAT CCTGGAGAGA GGCAAATCTA TGATCCAAGA CAGCTCTCTG
    GACCCTCAAG AGATAGAAGA AAGGCTTGGG CTACTGAAAA GCACATGGGA AGAGCTGGCA
    GCAGAAGGAG GAAAGCGCCA TGCCCGTTTA GAGGAATCTC TTGCTGCTCA GCAGTTTTAT
    GCTGATGCGG CAGAAGCTGA GGCCTGGATG GGAGAGCAAG AGTTACATAT GATGTCTGAA
    GAAAAAGCCA AGGATGAGAT TGGTGCCCAG GCTATGCTGA AGAAACACTT AGTAATGGAA
    CAGGCTCTTG ATGACTATGC AGACAACATC CATCAGCTTG CCAATTCAAG CCGCGACATG
    GTCTCCAGAG GTCATCCAGA AAGTGATCGT ATTACCCTTC GTCAAGGGCA AGTGGACAAG
    CTATATGCCA GTCTTAAAGA CCTGGCAGAG GAGAGACAAG GAAAGCTACA AGAGCACCAC
    AGACTTTGCC AACTCAAGAG AGATGTCGAA GACCTTGAGC ACTGGATCTC TGAACGTGAG
    GTGGTTGCAG CTTCTCATGA ACTTGGGCAA GATTATGAGC ATGTAACGAT GCTGAGAGAC
    AAATTCCGTG AGTTCTCCCG TGACACCAGT AACATTGGTC AAGAGCGGGT GGATGCTGTC
    AATCTGATGG CAGATGATTT AATTTCATCT GGACATTCGG AAAATGCTAC TGTAGCAGAT
    TGGAAAGACC AGCTAAATGA GGCATGGGCT GATCTTCTTG AGATGATCGA CACTCGAAGT
    CAGATGCTGG CAGCCTCATA TGAACTTCAC CGTTTCTACC AGGATGCAAA AGAAACCCTA
    GGTCAGATCC AAGACAAGCA GAAACAAATA CCAGAGGAGA CAGGCAGAGA TCTGAATACA
    GCAGAAGCCA TGCAGCGCAT GCACACCGCT TTTGAACATG ATATCCAAGC CCTGAGTGCA
    CAGGTGAAGC AAGTCCAGGA AGATGCTTCT CGCCTCCAAA AAGCTTATGC TGGTGAAAAG
    GCAGCAGACA TTCATCGCCA TGAGCAGGCA GTATCGGAAG CTTGGGGAGA GTTGCAGAAT
    GCTAGTGGTG CCAGGCGGCA GCTTCTTCTT GACACTGTGG AGAAATTCCG TTTCTTCAGA
    ATGGTGCGAG ATCTCATGTT ATGGATGGAT GATATAAACC TGCTAATTGA TGCACAGGAG
    AAGCCAAGAG ATGTATCCTC AGCTGATTTA GTCATCCAAA ACCACCTAGG CATCAAGGCA
    GAAGTGGATG CGCGAGCTGA CAGCTTTACT ACATGCATAG ATCTTGGCAA CTCTCTCCTA
    GAGAAGAATC ATTATGCCTC TGACAAGATT TCAGAGAAAC TGGAGCAATT GCAGGAAAGA
    AGGCAGGAGA CTGAAAACAA ATGGAAAGAT AAAATGGCAT GGCTGAGAGT TGTCCACGAA
    GTGCTGATGT TTGGAAGAGA TGCAAGTGTA GCTGAGGCTT GGCTGGCCAG CCAGGAGCCC
    ATTGTCCGGT CGTCAGAACT TGGTGGCAAT GTCGATGAAG TAGAATCCCT GATAAAAAGA
    CACGAAGCCT TTCAGAAAGC AGTTTCTACA TGGGATGAGA GATTTGTTCT TCTTGAAAAA
    CTAACCACGT TAGAAGAAAT TGAAATGCGT CGGAAGCGGG AAGAGGAAGA AAGAAAGAAA
    AGACCACCAA CACCACCTCT ACCAGTAGAA GAGCCTCGAG AGGAAGTTGT AAAAACCCAA
    GTTTCCCAGA CTATTGAAGG ACAGACTGAT CCAGCCAATA TACTTCAAGA AAGGATAAAT
    GGCATGCAGA ATTCATTCCA GGAGCAAGTA TCTGTTAATG GACTGTGCAC AGAGTCTGAT
    TCTCCACAGC CATCGTCCGA TACACAGAGG TTAGAAGACA GCATCTCTGA AAATCAGGGT
    CCGGAACAGT CAGATGTAAC CAATGGAGCC AGGACTGAGG TTATTACCAA AGATGGAAGT
    CCAACACCTT CTCCACAACT TGTGGCTAGG CCAACCTTCT CCCCGATAAA CCTCCAGTAT
    TCTACTTTGC CATCTAGCCC CACTGAACCA TTGGCATCTG AGCAAATGGA AGGAATGCTG
    CACCGCAAGC AGGAAATGGA TTCTCACAAC AAGAAGGCTG CGAACAGGTC CTGGCAGAAT
    GTTTACTGTG CACTGAAAAG GGGCAGTCTG GGTTTCTACA AGGATGCTAA AAGTGCAGCC
    AGTGGCGTTT CTTATCATTC GGAGCCCTCC GTCCAACTTA ATGGAGCCCA GTGCAGTGTT
    GCTCATGACT ACAAGAAACG CAAGAATGTC TTCAAGCTAA GCCTTCTTGA TGGCAAAGAA
    TATCTGTTCC AAGCTAAGGA TGAGGCTGAA ATGAGTAGTT GGATGCGAGT TATAAACGCC
    TCCCTAACTT CCTCCCCGGG AGACCCCTCA GACCATTCAC CGGGCATCAT GAAGGGTATG
    ACCCGGGCCA TGACAATGCC ACCAGTATCA CCCAACAGTG GTACAGAGGG ATCAGTCACA
    TTGCGCAACA AGGACGGGAA AGAAAAAGAC CGGGAAAAGA GATTCAGTTT CTTTAAGAAG
    AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004913752.1
    CDS247..7452
    Translation

    Target ORF information:

    RefSeq Version XM_004913695.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004913695.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
    GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA
    AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGATG AAAGAGAAGC TGTGCAGAAG
    AAGACATTTA CTAAATGGGT CAACTCTCAC TTGGCGAGGG TCACCTGTAG AATTTCTGAT
    CTCTACAATG ATTTACGAGA TGGACGGATG TTAATTCGAC TGTTAGAAGT GCTATCTGGA
    GAGCAGCTGC CAAAACCCAC AAAAGGTCGA ATGCGAATCC ACTGTTTGGA AAATGTAGAC
    AAAGCCCTTC AATTTTTAAA AGAGCAGAAG GTACATTTGG AGAACATGGG ATCACATGAC
    ATTGTGGATG GAAACCATCG ATTAACCCTA GGGTTAATCT GGACTATTAT TTTAAGGTTT
    CAGATCCAAG ATATCAGTGT AGAGACAGAA GATAATAAAG AAAAGAAGTC TGCTAAAGAT
    GCCTTACTGC TTTGGTGCCA GATGAAGACT GCTGGGTATC CAAATGTCAA TGTTCACAAC
    TTTACCACCA GCTGGCGAGA TGGACTTGCG TTTAATGCCA TTGTACACAA GCACAGACCA
    GATATAATTG ACTTTGACAC ACTGAAGAAG TCTAATGCAC ACCACAACCT CCAGAATGCC
    TTCAATGTAG CTGAAAGAGA GTTAGGTCTT ACTAAACTTC TGGATCCTGA AGATGTTAAT
    GTGGATCAGC CTGATGAGAA ATCCATCATA ACCTATGTGG CCACATTTTA TCACTATTTT
    TCCAAAATGA AAGCCCTAGC TGTGGAAGGA AAGCGTATTG GAAAGGTACT TGACCAAGCT
    ATTGAAGCTG ATCAGTTAAT TGAGAACTAT GAAGGACTGG CATCAGAACT CCTGCAATGG
    ATTGAACAGA TGATCATTAC TCTCAATGAC CGAAAACTGG CAAACTCTCT AAGTGGTGTT
    CAGAACCAGC TGCAGGCCTT CAACACCTAC AGGACTGTAG AAAAGCCGCC CAAGTTCACA
    GAAAAGGGCA ATTTGGAAGT TCTTCTCTTT ACCATACAGA GTAAAATGAG AGCTAATAAT
    CAAAAAGTGT ATACGCCCAG AGAAGGAAAG CTTATATCTG ATATAAACAA GGCATGGGAG
    CGACTAGAAA AGGCAGAGCA TGAACGTGAG CTTGCCCTTC GCAATGAACT TATACGCCAG
    GAAAAATTAG AGCAGTTGGC TGCACGATTT GACCGTAAAG CAGCCATGAG GGAAACCTGG
    CTAAGTGAAA ATCAGAGACT TGTAGCTCAG GATAACTTCG GTGTGGACTT AACTGCGGTG
    GAGGCAGCGC TACGGAAACA TGAAGCTATT GAAACTGATA TATTGGCTTA CAGTGAGCGC
    GTGCAAGCAG TGAATGCAGT GGCTGATGAG CTGGAAGCAG AGAATTACCA TGATGTCCGG
    AGGATTTTGG CTCGGAAGTG CAATGTGATT CGTCTCTCTG ATTATTTAAG AGAGCTTGTC
    ACTGCCAGGA GGCAAAGGCT TCATATGAAC CTAGAATTGC AGAGGATGTT CCAGGATTTA
    CATTATCTCA TGGACTGGAC TGAAGATATG AAGGGCCGTC TGCAGTCAGA AGATTATGGC
    AAGCATCTTC ATGGCGTGGA AGACCTTCTC CAGACTCACA CTCTTGTGGA GGCAGACATT
    GCAGTTCAGG CAGAACGTGT GAAATCAGTT ACAGCTGTAG CTCAGAAATT TATGACAGAA
    GATGAAGGCT ACAAGCCTTG CGACCCAGAG CTAGTGCGCA ACCGGGTGCA TCTCTTGGAA
    CAGCGCTATG CAGAGCTGTG TAATCTTTCT GCGGCTCGGA GATCAAAGCT GGAAGAATCC
    CGCCGTCTCT GGAAATTTTT CTGGGACATG GGAGAGGAGG AAGCCTGGAT CCGTGAGCAG
    AGTAGATTAC TACTTTCTGA TGACTATGGG AAAGACCTTA CCAGCTCACT TAGGTTGATC
    AGCAAGCATA ATGCCTTCAG AGGTGAGATG AGTGGACACC GTGGGCCGTT GCAGCAGACT
    GTGGCTGAGG GTGAGGAACT AATATCCAAA GGTCACTTTG GTGCAGGAGA GATTGAGGAA
    AGGATAAAAG AAGTGAAAGG CCAGTGGACA GAGATGGAGG CCTTAGCTGA AGACAGGGAA
    AGGAGGCTAA GAGAGGCCTA CAGCTATTAT CAGTTTCAAG CTGATGCCAA TGATGCTGAG
    GCTTGGATCC TGGACACTTT ACGCCTTGTT TCTACCAGTG AAGTAGGACA TGATGAGTAT
    TCCACACAGA CACTGGTGAA AAAGCACAAA GAAATAGAAG AGGAGATCCT TAGCCACCGA
    CCAGCTATTG ATGCGCTGCA TGAACAGGCC CGTGGCCTTC CACCTGCTTA CTCTCATTCA
    CCTGAAGTTG ATGGAAGGTT ACCAGCCCTG GAGCAGCGCT ATGAGGAATT GGTGGCTCTT
    GCTGCTCACC GCAAGCAGGC TCTGCAAGAT TCTCTGGCTC TTCATAAAAT GTTTAGTGAA
    GCTGATGCTT GCCTTTTGTG GATTGATGAG AAAGAACAGT GGCTTAATGT CACAGAGATT
    CCAGAAAAAC TTGAGGACCT AGAAGTAGTT CAACAAAGGT TTGAAACTCT AGAGGCAGAG
    ATGAATAATT TGGCATCCCG GATTGCATCT GTAAATGAAG TTGCTGGACA GTTGCTGACT
    ACAGACCAGA GAAATGAAAA CAGCATCCGA GACATGCAAG ATCAACTTAA TTCTCGGTGG
    AATGAATTCC GCAAGCTTGT TGACCAAAAG AAGGGTGCTT TGGATTCTGC TCTGGATATA
    CAGAATTATC ATTTGGAGTG CAATGAGACT GTGTGCTGGA TGAGGGAGAA GACAAAGGTT
    ATTGAATCCA CACAGAGCCT AGGGAATGAT CTTGCAGGAG TAACAGCCCT CCAGAGAAAA
    CTTACTGGAA TGGAGCGTGA TCTGAAAGCC ATTCAGGGAA AAGTGGGTGA CCTGCGTTCA
    GAGGCAGAAA AGCTTGCAGA TGGGCACCCA CAGAAAGCAC CGGCCATAAT TGGCCGCTTA
    TCAGATATTG ATGAGACCTG GGGAGAACTA AAGGACACTA TGAAAAGAAG AGAAGAAACT
    TTAGGTGAAG CAAGCAAGCT GCAGGGATTT TTGAGAGACC TTGATGATTT CCAGAGCTGG
    TTGTCCCGGG CCCAGACAGC AGTGGCATCT GAGGATGTTC CAGCTGACCT AGCAGAAGCA
    GAGCGCCTTC TTGCACAACA TGAAGGCATA AAAAATGAGA TTGATCATTA TGGAGCTGAC
    TACCACAGAG TGAGAGATGT TGGTGAAGAA GTCACTCGTG GGCAAACAGA TGCCCAATAT
    ATGTTCTTGC AACAGAGACT GAAGGCGCTA GACACTGGCT GGAATGAGCT TGGACAGATG
    TGGGAAAACA GGCACCAGTT GTTGTCCCAG GGTCACAGCT TCCAGCAGTT TTTGAAAGAT
    ACAAAACAAG TGGAGGGGTT CCTTAATAAC CAGGAGTATG TCCTTTCCCA TACCGAAATG
    CCAAGTACGC TACAGGCAGC TGAAGCTGCC ATCAAACGCC ATGAAGATTT CCTCACAACC
    ATGGATGCCA ATGAAGAGAA GATACAGGGT CTTGTAGAAT CTGGGAGGAA GCTAATTGAG
    GAACAGAATG TCAATTCTGA GAAGATTCAA GAAAAAGTCA CTTCTATTGA TGAGAGACAC
    AAAAAGAACC AAAAAACTGC TAGCGAAGTA TTAACTCGTT TGAAGGACAA CCGTGATCTT
    CAACATTTCC TTCAAGACTG TCAAGAGCTC ACCCTCTGGA TCAATGAAAA AATGCTTACT
    GCTCAAGATG TTTCTTATGA TGAAGCTCGA AACCTGCATA GCAAGTGGCA AAAGCATCAG
    GCATTCATGG CAGAACTGGC CTCAAATAAA GACTGGCTTG ACAAAATAGA CAAGGAAGGG
    CAGCAGCTCA TGTCAGAAAA ACCGGAGCTT GAACCAGTAG TGAGAGAAAA ACTAGACACT
    CTGCATAAGC TGTGGAATGA CTTGGAATCT ACCACACAAA GTAAAGCTCA GTGTCTGTTT
    GATGCCAACA GGGCAGAGTT ATTCACACAA AGCTGTTCTG CTCTGGAGAG TTGGCTTCAA
    AACCTCGAGT CACAACTACA GTCAGATCAT TATGGCAAAG ATCTGACCAG TGTCAACATC
    CTATTAAATA AACAGCAGAT GCTGGAGAAC CAAATGGAAG TGAGGGAGAA GGAGCTAGAA
    GCTATACAGT CCCAGGCACA GGCCCTTGGA CAGGAGGATG CCAACATAGT GGAGGTTGAC
    TCAAAGCATA AGGATGTAGC AGATAAATTT TCCAAGCTGC GTGCTCCTCT AAACAAGAGA
    AGAAAATTAC TTCTAGCCTC CAAGGAAGGA CATCAGTTTA ACAGGGACCT GGAAGATGAA
    ATACTTTGGG TAAAGGAGAA GATGCCAATG GCAATCTCCA CTGATCATGG AAAAGATCTC
    CCGACAGTCC AGCTTCTTAT CAAAAAGAAT CAGACACTCC AAAAGGAAAT TTTGGGACAT
    CAACCACGGA TTGATGACAT CCTGGAGAGA GGCAAATCTA TGATCCAAGA CAGCTCTCTG
    GACCCTCAAG AGATAGAAGA AAGGCTTGGG CTACTGAAAA GCACATGGGA AGAGCTGGCA
    GCAGAAGGAG GAAAGCGCCA TGCCCGTTTA GAGGAATCTC TTGCTGCTCA GCAGTTTTAT
    GCTGATGCGG CAGAAGCTGA GGCCTGGATG GGAGAGCAAG AGTTACATAT GATGTCTGAA
    GAAAAAGCCA AGGATGAGAT TGGTGCCCAG GCTATGCTGA AGAAACACTT AGTAATGGAA
    CAGGCTCTTG ATGACTATGC AGACAACATC CATCAGCTTG CCAATTCAAG CCGCGACATG
    GTCTCCAGAG GTCATCCAGA AAGTGATCGT ATTACCCTTC GTCAAGGGCA AGTGGACAAG
    CTATATGCCA GTCTTAAAGA CCTGGCAGAG GAGAGACAAG GAAAGCTACA AGAGCACCAC
    AGACTTTGCC AACTCAAGAG AGATGTCGAA GACCTTGAGC ACTGGATCTC TGAACGTGAG
    GTGGTTGCAG CTTCTCATGA ACTTGGGCAA GATTATGAGC ATGTAACGAT GCTGAGAGAC
    AAATTCCGTG AGTTCTCCCG TGACACCAGT AACATTGGTC AAGAGCGGGT GGATGCTGTC
    AATCTGATGG CAGATGATTT AATTTCATCT GGACATTCGG AAAATGCTAC TGTAGCAGAT
    TGGAAAGACC AGCTAAATGA GGCATGGGCT GATCTTCTTG AGATGATCGA CACTCGAAGT
    CAGATGCTGG CAGCCTCATA TGAACTTCAC CGTTTCTACC AGGATGCAAA AGAAACCCTA
    GGTCAGATCC AAGACAAGCA GAAACAAATA CCAGAGGAGA CAGGCAGAGA TCTGAATACA
    GCAGAAGCCA TGCAGCGCAT GCACACCGCT TTTGAACATG ATATCCAAGC CCTGAGTGCA
    CAGGTGAAGC AAGTCCAGGA AGATGCTTCT CGCCTCCAAA AAGCTTATGC TGGTGAAAAG
    GCAGCAGACA TTCATCGCCA TGAGCAGGCA GTATCGGAAG CTTGGGGAGA GTTGCAGAAT
    GCTAGTGGTG CCAGGCGGCA GCTTCTTCTT GACACTGTGG AGAAATTCCG TTTCTTCAGA
    ATGGTGCGAG ATCTCATGTT ATGGATGGAT GATATAAACC TGCTAATTGA TGCACAGGAG
    AAGCCAAGAG ATGTATCCTC AGCTGATTTA GTCATCCAAA ACCACCTAGG CATCAAGGCA
    GAAGTGGATG CGCGAGCTGA CAGCTTTACT ACATGCATAG ATCTTGGCAA CTCTCTCCTA
    GAGAAGAATC ATTATGCCTC TGACAAGATT TCAGAGAAAC TGGAGCAATT GCAGGAAAGA
    AGGCAGGAGA CTGAAAACAA ATGGAAAGAT AAAATGGCAT GGCTGAGAGT TGTCCACGAA
    GTGCTGATGT TTGGAAGAGA TGCAAGTGTA GCTGAGGCTT GGCTGGCCAG CCAGGAGCCC
    ATTGTCCGGT CGTCAGAACT TGGTGGCAAT GTCGATGAAG TAGAATCCCT GATAAAAAGA
    CACGAAGCCT TTCAGAAAGC AGTTTCTACA TGGGATGAGA GATTTGTTCT TCTTGAAAAA
    CTAACCACGT TAGAAGAAAT TGAAATGCGT CGGAAGCGGG AAGAGGAAGA AAGAAAGAAA
    AGACCACCAA CACCACCTCT ACCAGTAGAA GAGCCTCGAG AGGAAGTTGT AAAAACCCAA
    GTTTCCCAGA CTATTGAAGG ACAGACTGAT CCAGCCAATA TACTTCAAGA AAGGATAAAT
    GGCATGCAGA ATTCATTCCA GGAGCAAGTA TCTGTTAATG GACTGTGCAC AGAGTCTGAT
    TCTCCACAGC CATCGTCCGA TACACAGAGG TTAGAAGACA GCATCTCTGA AAATCAGGGT
    CCGGAACAGT CAGATGTAAC CAATGGAGCC AGGACTGAGG TTATTACCAA AGATGGAAGT
    CCAACACCTT CTCCACAACT TGTGGCTAGG CCAACCTTCT CCCCGATAAA CCTCCAGTAT
    TCTACTTTGC CATCTAGCCC CACTGAACCA TTGGCATCTG AGCAAATGGA AGGAATGCTG
    CACCGCAAGC AGGAAATGGA TTCTCACAAC AAGAAGGCTG CGAACAGGTC CTGGCAGAAT
    GTTTACTGTG CACTGAAAAG GGGCAGTCTG GGTTTCTACA AGGATGCTAA AAGTGCAGCC
    AGTGGCGTTT CTTATCATTC GGAGCCCTCC GTCCAACTTA ATGGAGCCCA GTGCAGTGTT
    GCTCATGACT ACAAGAAACG CAAGAATGTC TTCAAGCTAA GCCTTCTTGA TGGCAAAGAA
    TATCTGTTCC AAGCTAAGGA TGAGGCTGAA ATGAGTAGTT GGATGCGAGT TATAAACGCC
    TCCCTAACTT CCTCCCCGGG AGACCCCTCA GACCATTCAC CGGGCATCAT GAAGGGTATG
    ACCCGGGCCA TGACAATGCC ACCAGTATCA CCCAACAGTG GTACAGAGGG ATCAGTCACA
    TTGCGCAACA AGGACGGGAA AGAAAAAGAC CGGGAAAAGA GATTCAGTTT CTTTAAGAAG
    AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33551
    Clone ID Related Accession (Same CDS sequence) XM_012962405.2
    Accession Version XM_012962405.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spectrin beta chain, non-erythrocytic 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012962405.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
    GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA
    AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGAAA TGGATTTTGT CCCCCGATCT
    GGCTCCCCTC TCCTATCCCC TGCGGCTTTT GTAAATGCAG TGCAATACGC TAACGTGCTG
    GAAGGAAGGT TCAAGCAGCT GCAAGATGAA AGAGAAGCTG TGCAGAAGAA GACATTTACT
    AAATGGGTCA ACTCTCACTT GGCGAGGGTC ACCTGTAGAA TTTCTGATCT CTACAATGAT
    TTACGAGATG GACGGATGTT AATTCGACTG TTAGAAGTGC TATCTGGAGA GCAGCTGCCA
    AAACCCACAA AAGGTCGAAT GCGAATCCAC TGTTTGGAAA ATGTAGACAA AGCCCTTCAA
    TTTTTAAAAG AGCAGAAGGT ACATTTGGAG AACATGGGAT CACATGACAT TGTGGATGGA
    AACCATCGAT TAACCCTAGG GTTAATCTGG ACTATTATTT TAAGGTTTCA GATCCAAGAT
    ATCAGTGTAG AGACAGAAGA TAATAAAGAA AAGAAGTCTG CTAAAGATGC CTTACTGCTT
    TGGTGCCAGA TGAAGACTGC TGGGTATCCA AATGTCAATG TTCACAACTT TACCACCAGC
    TGGCGAGATG GACTTGCGTT TAATGCCATT GTACACAAGC ACAGACCAGA TATAATTGAC
    TTTGACACAC TGAAGAAGTC TAATGCACAC CACAACCTCC AGAATGCCTT CAATGTAGCT
    GAAAGAGAGT TAGGTCTTAC TAAACTTCTG GATCCTGAAG ATGTTAATGT GGATCAGCCT
    GATGAGAAAT CCATCATAAC CTATGTGGCC ACATTTTATC ACTATTTTTC CAAAATGAAA
    GCCCTAGCTG TGGAAGGAAA GCGTATTGGA AAGGTACTTG ACCAAGCTAT TGAAGCTGAT
    CAGTTAATTG AGAACTATGA AGGACTGGCA TCAGAACTCC TGCAATGGAT TGAACAGATG
    ATCATTACTC TCAATGACCG AAAACTGGCA AACTCTCTAA GTGGTGTTCA GAACCAGCTG
    CAGGCCTTCA ACACCTACAG GACTGTAGAA AAGCCGCCCA AGTTCACAGA AAAGGGCAAT
    TTGGAAGTTC TTCTCTTTAC CATACAGAGT AAAATGAGAG CTAATAATCA AAAAGTGTAT
    ACGCCCAGAG AAGGAAAGCT TATATCTGAT ATAAACAAGG CATGGGAGCG ACTAGAAAAG
    GCAGAGCATG AACGTGAGCT TGCCCTTCGC AATGAACTTA TACGCCAGGA AAAATTAGAG
    CAGTTGGCTG CACGATTTGA CCGTAAAGCA GCCATGAGGG AAACCTGGCT AAGTGAAAAT
    CAGAGACTTG TAGCTCAGGA TAACTTCGGT GTGGACTTAA CTGCGGTGGA GGCAGCGCTA
    CGGAAACATG AAGCTATTGA AACTGATATA TTGGCTTACA GTGAGCGCGT GCAAGCAGTG
    AATGCAGTGG CTGATGAGCT GGAAGCAGAG AATTACCATG ATGTCCGGAG GATTTTGGCT
    CGGAAGTGCA ATGTGATTCG TCTCTCTGAT TATTTAAGAG AGCTTGTCAC TGCCAGGAGG
    CAAAGGCTTC ATATGAACCT AGAATTGCAG AGGATGTTCC AGGATTTACA TTATCTCATG
    GACTGGACTG AAGATATGAA GGGCCGTCTG CAGTCAGAAG ATTATGGCAA GCATCTTCAT
    GGCGTGGAAG ACCTTCTCCA GACTCACACT CTTGTGGAGG CAGACATTGC AGTTCAGGCA
    GAACGTGTGA AATCAGTTAC AGCTGTAGCT CAGAAATTTA TGACAGAAGA TGAAGGCTAC
    AAGCCTTGCG ACCCAGAGCT AGTGCGCAAC CGGGTGCATC TCTTGGAACA GCGCTATGCA
    GAGCTGTGTA ATCTTTCTGC GGCTCGGAGA TCAAAGCTGG AAGAATCCCG CCGTCTCTGG
    AAATTTTTCT GGGACATGGG AGAGGAGGAA GCCTGGATCC GTGAGCAGAG TAGATTACTA
    CTTTCTGATG ACTATGGGAA AGACCTTACC AGCTCACTTA GGTTGATCAG CAAGCATAAT
    GCCTTCAGAG GTGAGATGAG TGGACACCGT GGGCCGTTGC AGCAGACTGT GGCTGAGGGT
    GAGGAACTAA TATCCAAAGG TCACTTTGGT GCAGGAGAGA TTGAGGAAAG GATAAAAGAA
    GTGAAAGGCC AGTGGACAGA GATGGAGGCC TTAGCTGAAG ACAGGGAAAG GAGGCTAAGA
    GAGGCCTACA GCTATTATCA GTTTCAAGCT GATGCCAATG ATGCTGAGGC TTGGATCCTG
    GACACTTTAC GCCTTGTTTC TACCAGTGAA GTAGGACATG ATGAGTATTC CACACAGACA
    CTGGTGAAAA AGCACAAAGA AATAGAAGAG GAGATCCTTA GCCACCGACC AGCTATTGAT
    GCGCTGCATG AACAGGCCCG TGGCCTTCCA CCTGCTTACT CTCATTCACC TGAAGTTGAT
    GGAAGGTTAC CAGCCCTGGA GCAGCGCTAT GAGGAATTGG TGGCTCTTGC TGCTCACCGC
    AAGCAGGCTC TGCAAGATTC TCTGGCTCTT CATAAAATGT TTAGTGAAGC TGATGCTTGC
    CTTTTGTGGA TTGATGAGAA AGAACAGTGG CTTAATGTCA CAGAGATTCC AGAAAAACTT
    GAGGACCTAG AAGTAGTTCA ACAAAGGTTT GAAACTCTAG AGGCAGAGAT GAATAATTTG
    GCATCCCGGA TTGCATCTGT AAATGAAGTT GCTGGACAGT TGCTGACTAC AGACCAGAGA
    AATGAAAACA GCATCCGAGA CATGCAAGAT CAACTTAATT CTCGGTGGAA TGAATTCCGC
    AAGCTTGTTG ACCAAAAGAA GGGTGCTTTG GATTCTGCTC TGGATATACA GAATTATCAT
    TTGGAGTGCA ATGAGACTGT GTGCTGGATG AGGGAGAAGA CAAAGGTTAT TGAATCCACA
    CAGAGCCTAG GGAATGATCT TGCAGGAGTA ACAGCCCTCC AGAGAAAACT TACTGGAATG
    GAGCGTGATC TGAAAGCCAT TCAGGGAAAA GTGGGTGACC TGCGTTCAGA GGCAGAAAAG
    CTTGCAGATG GGCACCCACA GAAAGCACCG GCCATAATTG GCCGCTTATC AGATATTGAT
    GAGACCTGGG GAGAACTAAA GGACACTATG AAAAGAAGAG AAGAAACTTT AGGTGAAGCA
    AGCAAGCTGC AGGGATTTTT GAGAGACCTT GATGATTTCC AGAGCTGGTT GTCCCGGGCC
    CAGACAGCAG TGGCATCTGA GGATGTTCCA GCTGACCTAG CAGAAGCAGA GCGCCTTCTT
    GCACAACATG AAGGCATAAA AAATGAGATT GATCATTATG GAGCTGACTA CCACAGAGTG
    AGAGATGTTG GTGAAGAAGT CACTCGTGGG CAAACAGATG CCCAATATAT GTTCTTGCAA
    CAGAGACTGA AGGCGCTAGA CACTGGCTGG AATGAGCTTG GACAGATGTG GGAAAACAGG
    CACCAGTTGT TGTCCCAGGG TCACAGCTTC CAGCAGTTTT TGAAAGATAC AAAACAAGTG
    GAGGGGTTCC TTAATAACCA GGAGTATGTC CTTTCCCATA CCGAAATGCC AAGTACGCTA
    CAGGCAGCTG AAGCTGCCAT CAAACGCCAT GAAGATTTCC TCACAACCAT GGATGCCAAT
    GAAGAGAAGA TACAGGGTCT TGTAGAATCT GGGAGGAAGC TAATTGAGGA ACAGAATGTC
    AATTCTGAGA AGATTCAAGA AAAAGTCACT TCTATTGATG AGAGACACAA AAAGAACCAA
    AAAACTGCTA GCGAAGTATT AACTCGTTTG AAGGACAACC GTGATCTTCA ACATTTCCTT
    CAAGACTGTC AAGAGCTCAC CCTCTGGATC AATGAAAAAA TGCTTACTGC TCAAGATGTT
    TCTTATGATG AAGCTCGAAA CCTGCATAGC AAGTGGCAAA AGCATCAGGC ATTCATGGCA
    GAACTGGCCT CAAATAAAGA CTGGCTTGAC AAAATAGACA AGGAAGGGCA GCAGCTCATG
    TCAGAAAAAC CGGAGCTTGA ACCAGTAGTG AGAGAAAAAC TAGACACTCT GCATAAGCTG
    TGGAATGACT TGGAATCTAC CACACAAAGT AAAGCTCAGT GTCTGTTTGA TGCCAACAGG
    GCAGAGTTAT TCACACAAAG CTGTTCTGCT CTGGAGAGTT GGCTTCAAAA CCTCGAGTCA
    CAACTACAGT CAGATCATTA TGGCAAAGAT CTGACCAGTG TCAACATCCT ATTAAATAAA
    CAGCAGATGC TGGAGAACCA AATGGAAGTG AGGGAGAAGG AGCTAGAAGC TATACAGTCC
    CAGGCACAGG CCCTTGGACA GGAGGATGCC AACATAGTGG AGGTTGACTC AAAGCATAAG
    GATGTAGCAG ATAAATTTTC CAAGCTGCGT GCTCCTCTAA ACAAGAGAAG AAAATTACTT
    CTAGCCTCCA AGGAAGGACA TCAGTTTAAC AGGGACCTGG AAGATGAAAT ACTTTGGGTA
    AAGGAGAAGA TGCCAATGGC AATCTCCACT GATCATGGAA AAGATCTCCC GACAGTCCAG
    CTTCTTATCA AAAAGAATCA GACACTCCAA AAGGAAATTT TGGGACATCA ACCACGGATT
    GATGACATCC TGGAGAGAGG CAAATCTATG ATCCAAGACA GCTCTCTGGA CCCTCAAGAG
    ATAGAAGAAA GGCTTGGGCT ACTGAAAAGC ACATGGGAAG AGCTGGCAGC AGAAGGAGGA
    AAGCGCCATG CCCGTTTAGA GGAATCTCTT GCTGCTCAGC AGTTTTATGC TGATGCGGCA
    GAAGCTGAGG CCTGGATGGG AGAGCAAGAG TTACATATGA TGTCTGAAGA AAAAGCCAAG
    GATGAGATTG GTGCCCAGGC TATGCTGAAG AAACACTTAG TAATGGAACA GGCTCTTGAT
    GACTATGCAG ACAACATCCA TCAGCTTGCC AATTCAAGCC GCGACATGGT CTCCAGAGGT
    CATCCAGAAA GTGATCGTAT TACCCTTCGT CAAGGGCAAG TGGACAAGCT ATATGCCAGT
    CTTAAAGACC TGGCAGAGGA GAGACAAGGA AAGCTACAAG AGCACCACAG ACTTTGCCAA
    CTCAAGAGAG ATGTCGAAGA CCTTGAGCAC TGGATCTCTG AACGTGAGGT GGTTGCAGCT
    TCTCATGAAC TTGGGCAAGA TTATGAGCAT GTAACGATGC TGAGAGACAA ATTCCGTGAG
    TTCTCCCGTG ACACCAGTAA CATTGGTCAA GAGCGGGTGG ATGCTGTCAA TCTGATGGCA
    GATGATTTAA TTTCATCTGG ACATTCGGAA AATGCTACTG TAGCAGATTG GAAAGACCAG
    CTAAATGAGG CATGGGCTGA TCTTCTTGAG ATGATCGACA CTCGAAGTCA GATGCTGGCA
    GCCTCATATG AACTTCACCG TTTCTACCAG GATGCAAAAG AAACCCTAGG TCAGATCCAA
    GACAAGCAGA AACAAATACC AGAGGAGACA GGCAGAGATC TGAATACAGC AGAAGCCATG
    CAGCGCATGC ACACCGCTTT TGAACATGAT ATCCAAGCCC TGAGTGCACA GGTGAAGCAA
    GTCCAGGAAG ATGCTTCTCG CCTCCAAAAA GCTTATGCTG GTGAAAAGGC AGCAGACATT
    CATCGCCATG AGCAGGCAGT ATCGGAAGCT TGGGGAGAGT TGCAGAATGC TAGTGGTGCC
    AGGCGGCAGC TTCTTCTTGA CACTGTGGAG AAATTCCGTT TCTTCAGAAT GGTGCGAGAT
    CTCATGTTAT GGATGGATGA TATAAACCTG CTAATTGATG CACAGGAGAA GCCAAGAGAT
    GTATCCTCAG CTGATTTAGT CATCCAAAAC CACCTAGGCA TCAAGGCAGA AGTGGATGCG
    CGAGCTGACA GCTTTACTAC ATGCATAGAT CTTGGCAACT CTCTCCTAGA GAAGAATCAT
    TATGCCTCTG ACAAGATTTC AGAGAAACTG GAGCAATTGC AGGAAAGAAG GCAGGAGACT
    GAAAACAAAT GGAAAGATAA AATGGCATGG CTGAGAGTTG TCCACGAAGT GCTGATGTTT
    GGAAGAGATG CAAGTGTAGC TGAGGCTTGG CTGGCCAGCC AGGAGCCCAT TGTCCGGTCG
    TCAGAACTTG GTGGCAATGT CGATGAAGTA GAATCCCTGA TAAAAAGACA CGAAGCCTTT
    CAGAAAGCAG TTTCTACATG GGATGAGAGA TTTGTTCTTC TTGAAAAACT AACCACGTTA
    GAAGAAATTG AAATGCGTCG GAAGCGGGAA GAGGAAGAAA GAAAGAAAAG ACCACCAACA
    CCACCTCTAC CAGTAGAAGA GCCTCGAGAG GAAGTTGTAA AAACCCAAGT TTCCCAGACT
    ATTGAAGGAC AGACTGATCC AGCCAATATA CTTCAAGAAA GGATAAATGG CATGCAGAAT
    TCATTCCAGG AGCAAGTATC TGTTAATGGA CTGTGCACAG AGTCTGATTC TCCACAGCCA
    TCGTCCGATA CACAGAGGTT AGAAGACAGC ATCTCTGAAA ATCAGGGTCC GGAACAGTCA
    GATGTAACCA ATGGAGCCAG GACTGAGGTT ATTACCAAAG ATGGAAGTCC AACACCTTCT
    CCACAACTTG TGGCTAGGCC AACCTTCTCC CCGATAAACC TCCAGTATTC TACTTTGCCA
    TCTAGCCCCA CTGAACCATT GGCATCTGAG CAAATGGAAG GAATGCTGCA CCGCAAGCAG
    GAAATGGATT CTCACAACAA GAAGGCTGCG AACAGGTCCT GGCAGAATGT TTACTGTGCA
    CTGAAAAGGG GCAGTCTGGG TTTCTACAAG GATGCTAAAA GTGCAGCCAG TGGCGTTTCT
    TATCATTCGG AGCCCTCCGT CCAACTTAAT GGAGCCCAGT GCAGTGTTGC TCATGACTAC
    AAGAAACGCA AGAATGTCTT CAAGCTAAGC CTTCTTGATG GCAAAGAATA TCTGTTCCAA
    GCTAAGGATG AGGCTGAAAT GAGTAGTTGG ATGCGAGTTA TAAACGCCTC CCTAACTTCC
    TCCCCGGGAG ACCCCTCAGA CCATTCACCG GGCATCATGA AGGGTATGAC CCGGGCCATG
    ACAATGCCAC CAGTATCACC CAACAGTGGT ACAGAGGGAT CAGTCACATT GCGCAACAAG
    GACGGGAAAG AAAAAGACCG GGAAAAGAGA TTCAGTTTCT TTAAGAAGAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012817859.1
    CDS66..7379
    Translation

    Target ORF information:

    RefSeq Version XM_012962405.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spectrin, beta, non-erythrocytic 2 (sptbn2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012962405.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGAGCAGTC CACTGTCACC GACAGATTAT GACAGCCTGG AAATCCAGCA GCAGTATAAT 
    GACATCAACA ATCGATGGGA TCTGACAGAT GATGATTGGG ACAATGAGAA CAGTTCTGCA
    AGACTTTTTG AGAGGTCCCG GATCAAAGCA CTTGCAGAAA TGGATTTTGT CCCCCGATCT
    GGCTCCCCTC TCCTATCCCC TGCGGCTTTT GTAAATGCAG TGCAATACGC TAACGTGCTG
    GAAGGAAGGT TCAAGCAGCT GCAAGATGAA AGAGAAGCTG TGCAGAAGAA GACATTTACT
    AAATGGGTCA ACTCTCACTT GGCGAGGGTC ACCTGTAGAA TTTCTGATCT CTACAATGAT
    TTACGAGATG GACGGATGTT AATTCGACTG TTAGAAGTGC TATCTGGAGA GCAGCTGCCA
    AAACCCACAA AAGGTCGAAT GCGAATCCAC TGTTTGGAAA ATGTAGACAA AGCCCTTCAA
    TTTTTAAAAG AGCAGAAGGT ACATTTGGAG AACATGGGAT CACATGACAT TGTGGATGGA
    AACCATCGAT TAACCCTAGG GTTAATCTGG ACTATTATTT TAAGGTTTCA GATCCAAGAT
    ATCAGTGTAG AGACAGAAGA TAATAAAGAA AAGAAGTCTG CTAAAGATGC CTTACTGCTT
    TGGTGCCAGA TGAAGACTGC TGGGTATCCA AATGTCAATG TTCACAACTT TACCACCAGC
    TGGCGAGATG GACTTGCGTT TAATGCCATT GTACACAAGC ACAGACCAGA TATAATTGAC
    TTTGACACAC TGAAGAAGTC TAATGCACAC CACAACCTCC AGAATGCCTT CAATGTAGCT
    GAAAGAGAGT TAGGTCTTAC TAAACTTCTG GATCCTGAAG ATGTTAATGT GGATCAGCCT
    GATGAGAAAT CCATCATAAC CTATGTGGCC ACATTTTATC ACTATTTTTC CAAAATGAAA
    GCCCTAGCTG TGGAAGGAAA GCGTATTGGA AAGGTACTTG ACCAAGCTAT TGAAGCTGAT
    CAGTTAATTG AGAACTATGA AGGACTGGCA TCAGAACTCC TGCAATGGAT TGAACAGATG
    ATCATTACTC TCAATGACCG AAAACTGGCA AACTCTCTAA GTGGTGTTCA GAACCAGCTG
    CAGGCCTTCA ACACCTACAG GACTGTAGAA AAGCCGCCCA AGTTCACAGA AAAGGGCAAT
    TTGGAAGTTC TTCTCTTTAC CATACAGAGT AAAATGAGAG CTAATAATCA AAAAGTGTAT
    ACGCCCAGAG AAGGAAAGCT TATATCTGAT ATAAACAAGG CATGGGAGCG ACTAGAAAAG
    GCAGAGCATG AACGTGAGCT TGCCCTTCGC AATGAACTTA TACGCCAGGA AAAATTAGAG
    CAGTTGGCTG CACGATTTGA CCGTAAAGCA GCCATGAGGG AAACCTGGCT AAGTGAAAAT
    CAGAGACTTG TAGCTCAGGA TAACTTCGGT GTGGACTTAA CTGCGGTGGA GGCAGCGCTA
    CGGAAACATG AAGCTATTGA AACTGATATA TTGGCTTACA GTGAGCGCGT GCAAGCAGTG
    AATGCAGTGG CTGATGAGCT GGAAGCAGAG AATTACCATG ATGTCCGGAG GATTTTGGCT
    CGGAAGTGCA ATGTGATTCG TCTCTCTGAT TATTTAAGAG AGCTTGTCAC TGCCAGGAGG
    CAAAGGCTTC ATATGAACCT AGAATTGCAG AGGATGTTCC AGGATTTACA TTATCTCATG
    GACTGGACTG AAGATATGAA GGGCCGTCTG CAGTCAGAAG ATTATGGCAA GCATCTTCAT
    GGCGTGGAAG ACCTTCTCCA GACTCACACT CTTGTGGAGG CAGACATTGC AGTTCAGGCA
    GAACGTGTGA AATCAGTTAC AGCTGTAGCT CAGAAATTTA TGACAGAAGA TGAAGGCTAC
    AAGCCTTGCG ACCCAGAGCT AGTGCGCAAC CGGGTGCATC TCTTGGAACA GCGCTATGCA
    GAGCTGTGTA ATCTTTCTGC GGCTCGGAGA TCAAAGCTGG AAGAATCCCG CCGTCTCTGG
    AAATTTTTCT GGGACATGGG AGAGGAGGAA GCCTGGATCC GTGAGCAGAG TAGATTACTA
    CTTTCTGATG ACTATGGGAA AGACCTTACC AGCTCACTTA GGTTGATCAG CAAGCATAAT
    GCCTTCAGAG GTGAGATGAG TGGACACCGT GGGCCGTTGC AGCAGACTGT GGCTGAGGGT
    GAGGAACTAA TATCCAAAGG TCACTTTGGT GCAGGAGAGA TTGAGGAAAG GATAAAAGAA
    GTGAAAGGCC AGTGGACAGA GATGGAGGCC TTAGCTGAAG ACAGGGAAAG GAGGCTAAGA
    GAGGCCTACA GCTATTATCA GTTTCAAGCT GATGCCAATG ATGCTGAGGC TTGGATCCTG
    GACACTTTAC GCCTTGTTTC TACCAGTGAA GTAGGACATG ATGAGTATTC CACACAGACA
    CTGGTGAAAA AGCACAAAGA AATAGAAGAG GAGATCCTTA GCCACCGACC AGCTATTGAT
    GCGCTGCATG AACAGGCCCG TGGCCTTCCA CCTGCTTACT CTCATTCACC TGAAGTTGAT
    GGAAGGTTAC CAGCCCTGGA GCAGCGCTAT GAGGAATTGG TGGCTCTTGC TGCTCACCGC
    AAGCAGGCTC TGCAAGATTC TCTGGCTCTT CATAAAATGT TTAGTGAAGC TGATGCTTGC
    CTTTTGTGGA TTGATGAGAA AGAACAGTGG CTTAATGTCA CAGAGATTCC AGAAAAACTT
    GAGGACCTAG AAGTAGTTCA ACAAAGGTTT GAAACTCTAG AGGCAGAGAT GAATAATTTG
    GCATCCCGGA TTGCATCTGT AAATGAAGTT GCTGGACAGT TGCTGACTAC AGACCAGAGA
    AATGAAAACA GCATCCGAGA CATGCAAGAT CAACTTAATT CTCGGTGGAA TGAATTCCGC
    AAGCTTGTTG ACCAAAAGAA GGGTGCTTTG GATTCTGCTC TGGATATACA GAATTATCAT
    TTGGAGTGCA ATGAGACTGT GTGCTGGATG AGGGAGAAGA CAAAGGTTAT TGAATCCACA
    CAGAGCCTAG GGAATGATCT TGCAGGAGTA ACAGCCCTCC AGAGAAAACT TACTGGAATG
    GAGCGTGATC TGAAAGCCAT TCAGGGAAAA GTGGGTGACC TGCGTTCAGA GGCAGAAAAG
    CTTGCAGATG GGCACCCACA GAAAGCACCG GCCATAATTG GCCGCTTATC AGATATTGAT
    GAGACCTGGG GAGAACTAAA GGACACTATG AAAAGAAGAG AAGAAACTTT AGGTGAAGCA
    AGCAAGCTGC AGGGATTTTT GAGAGACCTT GATGATTTCC AGAGCTGGTT GTCCCGGGCC
    CAGACAGCAG TGGCATCTGA GGATGTTCCA GCTGACCTAG CAGAAGCAGA GCGCCTTCTT
    GCACAACATG AAGGCATAAA AAATGAGATT GATCATTATG GAGCTGACTA CCACAGAGTG
    AGAGATGTTG GTGAAGAAGT CACTCGTGGG CAAACAGATG CCCAATATAT GTTCTTGCAA
    CAGAGACTGA AGGCGCTAGA CACTGGCTGG AATGAGCTTG GACAGATGTG GGAAAACAGG
    CACCAGTTGT TGTCCCAGGG TCACAGCTTC CAGCAGTTTT TGAAAGATAC AAAACAAGTG
    GAGGGGTTCC TTAATAACCA GGAGTATGTC CTTTCCCATA CCGAAATGCC AAGTACGCTA
    CAGGCAGCTG AAGCTGCCAT CAAACGCCAT GAAGATTTCC TCACAACCAT GGATGCCAAT
    GAAGAGAAGA TACAGGGTCT TGTAGAATCT GGGAGGAAGC TAATTGAGGA ACAGAATGTC
    AATTCTGAGA AGATTCAAGA AAAAGTCACT TCTATTGATG AGAGACACAA AAAGAACCAA
    AAAACTGCTA GCGAAGTATT AACTCGTTTG AAGGACAACC GTGATCTTCA ACATTTCCTT
    CAAGACTGTC AAGAGCTCAC CCTCTGGATC AATGAAAAAA TGCTTACTGC TCAAGATGTT
    TCTTATGATG AAGCTCGAAA CCTGCATAGC AAGTGGCAAA AGCATCAGGC ATTCATGGCA
    GAACTGGCCT CAAATAAAGA CTGGCTTGAC AAAATAGACA AGGAAGGGCA GCAGCTCATG
    TCAGAAAAAC CGGAGCTTGA ACCAGTAGTG AGAGAAAAAC TAGACACTCT GCATAAGCTG
    TGGAATGACT TGGAATCTAC CACACAAAGT AAAGCTCAGT GTCTGTTTGA TGCCAACAGG
    GCAGAGTTAT TCACACAAAG CTGTTCTGCT CTGGAGAGTT GGCTTCAAAA CCTCGAGTCA
    CAACTACAGT CAGATCATTA TGGCAAAGAT CTGACCAGTG TCAACATCCT ATTAAATAAA
    CAGCAGATGC TGGAGAACCA AATGGAAGTG AGGGAGAAGG AGCTAGAAGC TATACAGTCC
    CAGGCACAGG CCCTTGGACA GGAGGATGCC AACATAGTGG AGGTTGACTC AAAGCATAAG
    GATGTAGCAG ATAAATTTTC CAAGCTGCGT GCTCCTCTAA ACAAGAGAAG AAAATTACTT
    CTAGCCTCCA AGGAAGGACA TCAGTTTAAC AGGGACCTGG AAGATGAAAT ACTTTGGGTA
    AAGGAGAAGA TGCCAATGGC AATCTCCACT GATCATGGAA AAGATCTCCC GACAGTCCAG
    CTTCTTATCA AAAAGAATCA GACACTCCAA AAGGAAATTT TGGGACATCA ACCACGGATT
    GATGACATCC TGGAGAGAGG CAAATCTATG ATCCAAGACA GCTCTCTGGA CCCTCAAGAG
    ATAGAAGAAA GGCTTGGGCT ACTGAAAAGC ACATGGGAAG AGCTGGCAGC AGAAGGAGGA
    AAGCGCCATG CCCGTTTAGA GGAATCTCTT GCTGCTCAGC AGTTTTATGC TGATGCGGCA
    GAAGCTGAGG CCTGGATGGG AGAGCAAGAG TTACATATGA TGTCTGAAGA AAAAGCCAAG
    GATGAGATTG GTGCCCAGGC TATGCTGAAG AAACACTTAG TAATGGAACA GGCTCTTGAT
    GACTATGCAG ACAACATCCA TCAGCTTGCC AATTCAAGCC GCGACATGGT CTCCAGAGGT
    CATCCAGAAA GTGATCGTAT TACCCTTCGT CAAGGGCAAG TGGACAAGCT ATATGCCAGT
    CTTAAAGACC TGGCAGAGGA GAGACAAGGA AAGCTACAAG AGCACCACAG ACTTTGCCAA
    CTCAAGAGAG ATGTCGAAGA CCTTGAGCAC TGGATCTCTG AACGTGAGGT GGTTGCAGCT
    TCTCATGAAC TTGGGCAAGA TTATGAGCAT GTAACGATGC TGAGAGACAA ATTCCGTGAG
    TTCTCCCGTG ACACCAGTAA CATTGGTCAA GAGCGGGTGG ATGCTGTCAA TCTGATGGCA
    GATGATTTAA TTTCATCTGG ACATTCGGAA AATGCTACTG TAGCAGATTG GAAAGACCAG
    CTAAATGAGG CATGGGCTGA TCTTCTTGAG ATGATCGACA CTCGAAGTCA GATGCTGGCA
    GCCTCATATG AACTTCACCG TTTCTACCAG GATGCAAAAG AAACCCTAGG TCAGATCCAA
    GACAAGCAGA AACAAATACC AGAGGAGACA GGCAGAGATC TGAATACAGC AGAAGCCATG
    CAGCGCATGC ACACCGCTTT TGAACATGAT ATCCAAGCCC TGAGTGCACA GGTGAAGCAA
    GTCCAGGAAG ATGCTTCTCG CCTCCAAAAA GCTTATGCTG GTGAAAAGGC AGCAGACATT
    CATCGCCATG AGCAGGCAGT ATCGGAAGCT TGGGGAGAGT TGCAGAATGC TAGTGGTGCC
    AGGCGGCAGC TTCTTCTTGA CACTGTGGAG AAATTCCGTT TCTTCAGAAT GGTGCGAGAT
    CTCATGTTAT GGATGGATGA TATAAACCTG CTAATTGATG CACAGGAGAA GCCAAGAGAT
    GTATCCTCAG CTGATTTAGT CATCCAAAAC CACCTAGGCA TCAAGGCAGA AGTGGATGCG
    CGAGCTGACA GCTTTACTAC ATGCATAGAT CTTGGCAACT CTCTCCTAGA GAAGAATCAT
    TATGCCTCTG ACAAGATTTC AGAGAAACTG GAGCAATTGC AGGAAAGAAG GCAGGAGACT
    GAAAACAAAT GGAAAGATAA AATGGCATGG CTGAGAGTTG TCCACGAAGT GCTGATGTTT
    GGAAGAGATG CAAGTGTAGC TGAGGCTTGG CTGGCCAGCC AGGAGCCCAT TGTCCGGTCG
    TCAGAACTTG GTGGCAATGT CGATGAAGTA GAATCCCTGA TAAAAAGACA CGAAGCCTTT
    CAGAAAGCAG TTTCTACATG GGATGAGAGA TTTGTTCTTC TTGAAAAACT AACCACGTTA
    GAAGAAATTG AAATGCGTCG GAAGCGGGAA GAGGAAGAAA GAAAGAAAAG ACCACCAACA
    CCACCTCTAC CAGTAGAAGA GCCTCGAGAG GAAGTTGTAA AAACCCAAGT TTCCCAGACT
    ATTGAAGGAC AGACTGATCC AGCCAATATA CTTCAAGAAA GGATAAATGG CATGCAGAAT
    TCATTCCAGG AGCAAGTATC TGTTAATGGA CTGTGCACAG AGTCTGATTC TCCACAGCCA
    TCGTCCGATA CACAGAGGTT AGAAGACAGC ATCTCTGAAA ATCAGGGTCC GGAACAGTCA
    GATGTAACCA ATGGAGCCAG GACTGAGGTT ATTACCAAAG ATGGAAGTCC AACACCTTCT
    CCACAACTTG TGGCTAGGCC AACCTTCTCC CCGATAAACC TCCAGTATTC TACTTTGCCA
    TCTAGCCCCA CTGAACCATT GGCATCTGAG CAAATGGAAG GAATGCTGCA CCGCAAGCAG
    GAAATGGATT CTCACAACAA GAAGGCTGCG AACAGGTCCT GGCAGAATGT TTACTGTGCA
    CTGAAAAGGG GCAGTCTGGG TTTCTACAAG GATGCTAAAA GTGCAGCCAG TGGCGTTTCT
    TATCATTCGG AGCCCTCCGT CCAACTTAAT GGAGCCCAGT GCAGTGTTGC TCATGACTAC
    AAGAAACGCA AGAATGTCTT CAAGCTAAGC CTTCTTGATG GCAAAGAATA TCTGTTCCAA
    GCTAAGGATG AGGCTGAAAT GAGTAGTTGG ATGCGAGTTA TAAACGCCTC CCTAACTTCC
    TCCCCGGGAG ACCCCTCAGA CCATTCACCG GGCATCATGA AGGGTATGAC CCGGGCCATG
    ACAATGCCAC CAGTATCACC CAACAGTGGT ACAGAGGGAT CAGTCACATT GCGCAACAAG
    GACGGGAAAG AAAAAGACCG GGAAAAGAGA TTCAGTTTCT TTAAGAAGAA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.