cftr cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following cftr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cftr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa54776 XM_031898971.1
Latest version!
Xenopus tropicalis cystic fibrosis transmembrane conductance regulator (cftr), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa54777 XM_012959952.3
Latest version!
Xenopus tropicalis cystic fibrosis transmembrane conductance regulator (cftr), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa33528 XM_012959952.2
Latest version!
Xenopus tropicalis cystic fibrosis transmembrane conductance regulator (cftr), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa54776
    Clone ID Related Accession (Same CDS sequence) XM_031898971.1
    Accession Version XM_031898971.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4458bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cystic fibrosis transmembrane conductance regulator isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCAGAGAT CCCCTCTGGA GAAAGCCAGC ATCTTTTCAC AGATTTTCTT TAGCTGGACC 
    AAGCCAATTT TGTGGAAAGG CTATAGGCAA CGGCTGGAAC TGTCAGACAT TTACCAGATT
    CACCCAGGGG ACTCTGCAGA TAACCTTTCA GAAAGGCTAG AAAGAGAATG GGACAGGGAG
    GTGGCAACAT CAAAGAAAAA CCCCAAATTA ATTAATGCAC TGAAACGATG TTTTTTCTGG
    AAATTTCTGT TCTATGGTAT CTTATTATAC CTTGGGGAAG TAACTAAAGC TGTTCAACCT
    CTTCTTCTTG GAAGAATCAT TGCTTCTTAT GACCGCGACA ATGAGCATGA ACGATCGATT
    GCTTATTACC TGGCAATTGG CCTATGTCTA CTTTTTGTAG TGAGAATGCT TCTTTTACAC
    CCTGCCATTT TTGGTCTACA CCATATTGGA ATGCAAATGA GAATAGCAAT GTTTAGTTTA
    ATTTACAAAA AGACTTTAAA ACTTTCAAGC AGAGTTCTCG ATAAAATAAG CACTGGACAG
    CTTGTAAGTC TTTTATCAAA CAACCTCAAT AAATTTGATG AGGGACTTGC TCTGGCTCAT
    TTTGTTTGGA TTGCACCTTT GCAAGTGCTG CTGTTGATGG GACTGCTGTG GGACTTACTT
    CAGGCCTCAG CATTTTGTGG GCTTGGCTTT CTTATCATCC TTGCATTGTT CCAAGCATGG
    CTTGGCCGTA TGATGATGAA ATATAGGGAT AAGAGGGCAG GAAAGATCAA TGAACGACTT
    GTGATAACAT CTGAAATCAT AGAGAACATC CAGTCAGTAA AGGCCTACTG TTGGGAGAGT
    GCAATGGAAA AAATAATTGA AACCATAAGA GAGACTGAAC TGAAGCTTAC TCGAAAAGCA
    GCATATGTGC GTTACTTCAA CAGCTCAGCA TTCTTTTTCT CTGGATTTTT TGTGGTGTTT
    CTTTGTATTG TGCCTCATCT ACTACTTGAT GGCATTTCAC TCCGAAAAAT ATTTACTACC
    ATTTCATTTA GCATAGTCCT GCGAATGGCA GTCACAAGGC AGTTCCCCTG GGCTGTACAG
    ACATGGTATG ATTCCCTTGG AGTGATAAAT AAAATACAGG AATTCCTGCA AAAGGAAGAA
    TATAAATCAC TGGAATATAA TTTAACAACC ACAGAAGTTG CAATGGAAAA TGTTTCAGCA
    TCTTGGGATG AGGGCATTGG AGAATTCTTT GAAAAAGCAA AGCAAGAAGT AAATGGAGGG
    AATATTTCTA ATGAGGATCC AAGTCCGTTC TTTAGTAACT TTTCCCTGCA TGTTGCTCCA
    GTCCTCAGAA ACATCAATTT TAAGATTGAA AAAGGGCAGC TTTTGGCAAT TGCTGGATCT
    ACAGGGGCTG GAAAGACTTC TCTATTGATG ATGATCATGG GAGAACTGGA GCCGTCAGCT
    GGTAAAATAA AGCATAGCGG AAGGATATCT TTTTCTCCTC AAGTATCTTG GATCATGCCG
    GGGACCATCA AAGAGAATAT AATATTTGGC GTTTCCTATG ACCAGTACAG ATACCTGAGT
    GTTATAAAAG CTTGTCAGCT GGAAGAGGAC ATTTCAAAAT TTCCAGAAAA AGATAATACT
    GTGTTAGGAG AAGGGGGAAT AACACTAAGT GGAGGCCAGC GTGCAAGGAT TTCACTTGCA
    AGAGCCGTAT ACAAAGATGC TGACCTCTAC TTGATGGATT CTCCATTCAG TTATTTAGAC
    TTGTTTACAG AAAAAGAAAT ATTTGAAAGT TGTGTTTGCA AGCTAATGGC TAATAAAACA
    AGGATTTTAG TTACATCTAA AGTTGAACAG CTTAAAAAAG CTGACAAAGT GCTGATTTTG
    CATGAAGGAA GCTGTTATTT CTATGGAACA TTTTCTGAAC TTCAAGATCA ACGTCCTGAG
    TTCAGCTCTC ATCTGACCGG ATTTGATCAC TTTAATGCAG AAAGAAGAAA CTCTATAATA
    ACTGAAACCC TGAGAAGATG CTCCATTGAT AGCGATCCAA CAGCTGTTAG GAATGAAGTC
    AAGAATAAGT CATTTAAGCA GGTTGCAGAT TTCAGTGAAA AAAGAAAGAA CTCTGTTATT
    AATCCTCGGA AATCCAGCAG AAAATTTTCT ATCATGCAAA AAAGTCAGCC ACAGATGAGT
    GGAATAGAAG AGGAAGATAT TCCCCCTAAT CAAGGAGAAC GAAAGCTTTC ATTAGTTCCT
    GAATCAGAAC AGGGAGAAGC CAGCCTGCCT AGAAGCAATG TTCTTAATAC AGGTCCTACA
    TTTCAAGGCC GCAGGAGACA GTCTGTACTA AATCTAATGA CTCGCACCTC TGTTTCTCAA
    GGTTCCAATG CCTTTGCAAC AAGAAATACA TCTGTTCGTA AAATGTCTGT AGGTTCTTTC
    TCCAACTCAT CTTTTGAAGT GGATATATAT AACAGACGAC TTTCACAAGA CAGTATTTTG
    GAAGTTAGTG AAGAAATCAA TGAAGAAGAT TTGAAGGAAT GTTTCCTGGA TGATACAGAC
    AGCCCATCAC CAACTACCAC ATGGAACACA TATATAAGAT TCCTTACAGC TCACAAGAAT
    TTCATTTTTA TTCTTGTATT TTGTATAGTG ATATTTTTAG CTGAGGTCAT TGCATCGTCA
    GCTGGGCTCT GGATTATTAA ACGAAATGCT CCTGTTAGCA ACATGACAAG TAATGAGAAT
    ATATCTGAAG CATCAGACAT CCTTTCTGTC ATTGTGACAC ACACTAGCTT TTACTATGTG
    TTTTACATTT ACGTTGGTGT GGCTGACTCC TTACTGGCTC TGGGAATATT CAGAGGTTTG
    CCTCTCGTAC ACAGTCTTAT ATCAGTCTCG AGGGTATTAC ACAAAAAAAT GCTGCATTCT
    ATTCTACATG CCCCAATGTC AACCTTCAAT ACAATGAGAG CTGGTCGGAT ACTTAACAGA
    TTTTCAAAAG ATACAGCAAT ATTGGATGAC ATTCTACCTC TGTCAATATT TGACTTCACA
    CAGCTTGTGC TTATTGTGAT TGGAGCAATA ACAGTGGTTT CATTACTTGA ACCTTACATT
    TTCCTGGCAA CTGTGCCTGT AATTGCTGCT TTTATTCTCT TGAGATCATA CTTTCTTCAC
    ACATCTCAGC AACTAAAACA GTTAGAATCT GAAGCCCGCA GTCCGATTTT TGCTCACCTT
    ATAACAAGTT TGAAGGGTTT ATGGACATTG AGAGCCTTCG GGAGACAACC ATATTTTGAA
    ACCCTTTTTC ACAAAGCACT GAATTTACAT ACTGCAAACT GGTTCCTCTA TCTGTCCACT
    CTACGCTGGT TCCAAATGAC AATAGAAATG ATTTTTGTGA TATTCTTTAT CGCTGTTTCA
    TTCATCTCCA TCGCAACATC AGGTGCTGGG GAAGAAAAAG TTGGAATTGT TCTGACATTG
    GCAATGAATA TCATGAATAC ATTACAGTGG GCTGTAAATG CTAGCATTGA TGTGGATAGC
    TTGATGCGTT CTGTCAGCAG AATTTTCAGG TTCATTGACA TGCCAAGAGA AGAGTTGATA
    CATGACAATA ACCACAAAGA TGACCAACTT TCAGAGGTTC TGATATATGA AAATGATTAC
    ATCAAGAAAA ACCAAGTCTG GCCATCTGGA GGTCAAATGA CAGTGAAAAA TCTTTCTGCA
    AAATATAAAG ATGGAGGCCA CACTGTGTTG GAAAATATAT CCTTCTCTTT AAACCCTGGA
    CAAAGGGTAG GCTTATTAGG AAGAACTGGT TCAGGTAAAA GCACCCTGTT GTCTGCCTTT
    TTGAGATTAT TATCAACCCA AGGAGACATT CAAATCGATG GGGTATCCTG GCAAACAATT
    CCACTTCAGA AATGGAGAAA GGCATTTGGA GTAGTTCCAC AGAAAGTATT TATATTTTCT
    GGAACTATTC GAAAAAACCT TGATCCCTAT GGAAAATGGA GTGATGAGGA ACTTTTGAAA
    GTCACAGAAG AGGTTGGTTT AAAGTCAATA ATTGATCAGT TTCCGGGACA GTTGGATTTT
    GTGCTTCTGG ATGGGGGTTG TGTCCTGAGC CATGGCCACA AGCAACTCGT ATGTTTGGCT
    AGGTCGGTTC TCAGCAAAGC AAAAATCTTA TTACTCGATG AGCCTAGTGC ACATTTGGAT
    CCAATAACAT TCCAAATAAT TAGGAAAACA CTAAAACATG CATTTGCAAA TTGCACAGTA
    ATTTTGTCAG AACACAGACT AGAGGCAATG TTGGAATGCC AGCACTTTCT GGTGATTGAA
    GACAATACAG TACGACAGTA TGACTCTATT CAAAAGCTTA TGAATGAGAA GAGTTTCTTC
    AAACAAGCCA TAAGCCAATC TGATCGCTTG AAACTATTCC CATTGCATCG TAGAAATTCA
    AGTAAACGAA AATCCAGACC CAAAATTTCT GCCTTACAAG AAGAGACAGA AGAGGAGGTT
    CAGGAAACAA GACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031754831.1
    CDS147..4604
    Translation

    Target ORF information:

    RefSeq Version XM_031898971.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis cystic fibrosis transmembrane conductance regulator (cftr), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031898971.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCAGAGAT CCCCTCTGGA GAAAGCCAGC ATCTTTTCAC AGATTTTCTT TAGCTGGACC 
    AAGCCAATTT TGTGGAAAGG CTATAGGCAA CGGCTGGAAC TGTCAGACAT TTACCAGATT
    CACCCAGGGG ACTCTGCAGA TAACCTTTCA GAAAGGCTAG AAAGAGAATG GGACAGGGAG
    GTGGCAACAT CAAAGAAAAA CCCCAAATTA ATTAATGCAC TGAAACGATG TTTTTTCTGG
    AAATTTCTGT TCTATGGTAT CTTATTATAC CTTGGGGAAG TAACTAAAGC TGTTCAACCT
    CTTCTTCTTG GAAGAATCAT TGCTTCTTAT GACCGCGACA ATGAGCATGA ACGATCGATT
    GCTTATTACC TGGCAATTGG CCTATGTCTA CTTTTTGTAG TGAGAATGCT TCTTTTACAC
    CCTGCCATTT TTGGTCTACA CCATATTGGA ATGCAAATGA GAATAGCAAT GTTTAGTTTA
    ATTTACAAAA AGACTTTAAA ACTTTCAAGC AGAGTTCTCG ATAAAATAAG CACTGGACAG
    CTTGTAAGTC TTTTATCAAA CAACCTCAAT AAATTTGATG AGGGACTTGC TCTGGCTCAT
    TTTGTTTGGA TTGCACCTTT GCAAGTGCTG CTGTTGATGG GACTGCTGTG GGACTTACTT
    CAGGCCTCAG CATTTTGTGG GCTTGGCTTT CTTATCATCC TTGCATTGTT CCAAGCATGG
    CTTGGCCGTA TGATGATGAA ATATAGGGAT AAGAGGGCAG GAAAGATCAA TGAACGACTT
    GTGATAACAT CTGAAATCAT AGAGAACATC CAGTCAGTAA AGGCCTACTG TTGGGAGAGT
    GCAATGGAAA AAATAATTGA AACCATAAGA GAGACTGAAC TGAAGCTTAC TCGAAAAGCA
    GCATATGTGC GTTACTTCAA CAGCTCAGCA TTCTTTTTCT CTGGATTTTT TGTGGTGTTT
    CTTTGTATTG TGCCTCATCT ACTACTTGAT GGCATTTCAC TCCGAAAAAT ATTTACTACC
    ATTTCATTTA GCATAGTCCT GCGAATGGCA GTCACAAGGC AGTTCCCCTG GGCTGTACAG
    ACATGGTATG ATTCCCTTGG AGTGATAAAT AAAATACAGG AATTCCTGCA AAAGGAAGAA
    TATAAATCAC TGGAATATAA TTTAACAACC ACAGAAGTTG CAATGGAAAA TGTTTCAGCA
    TCTTGGGATG AGGGCATTGG AGAATTCTTT GAAAAAGCAA AGCAAGAAGT AAATGGAGGG
    AATATTTCTA ATGAGGATCC AAGTCCGTTC TTTAGTAACT TTTCCCTGCA TGTTGCTCCA
    GTCCTCAGAA ACATCAATTT TAAGATTGAA AAAGGGCAGC TTTTGGCAAT TGCTGGATCT
    ACAGGGGCTG GAAAGACTTC TCTATTGATG ATGATCATGG GAGAACTGGA GCCGTCAGCT
    GGTAAAATAA AGCATAGCGG AAGGATATCT TTTTCTCCTC AAGTATCTTG GATCATGCCG
    GGGACCATCA AAGAGAATAT AATATTTGGC GTTTCCTATG ACCAGTACAG ATACCTGAGT
    GTTATAAAAG CTTGTCAGCT GGAAGAGGAC ATTTCAAAAT TTCCAGAAAA AGATAATACT
    GTGTTAGGAG AAGGGGGAAT AACACTAAGT GGAGGCCAGC GTGCAAGGAT TTCACTTGCA
    AGAGCCGTAT ACAAAGATGC TGACCTCTAC TTGATGGATT CTCCATTCAG TTATTTAGAC
    TTGTTTACAG AAAAAGAAAT ATTTGAAAGT TGTGTTTGCA AGCTAATGGC TAATAAAACA
    AGGATTTTAG TTACATCTAA AGTTGAACAG CTTAAAAAAG CTGACAAAGT GCTGATTTTG
    CATGAAGGAA GCTGTTATTT CTATGGAACA TTTTCTGAAC TTCAAGATCA ACGTCCTGAG
    TTCAGCTCTC ATCTGACCGG ATTTGATCAC TTTAATGCAG AAAGAAGAAA CTCTATAATA
    ACTGAAACCC TGAGAAGATG CTCCATTGAT AGCGATCCAA CAGCTGTTAG GAATGAAGTC
    AAGAATAAGT CATTTAAGCA GGTTGCAGAT TTCAGTGAAA AAAGAAAGAA CTCTGTTATT
    AATCCTCGGA AATCCAGCAG AAAATTTTCT ATCATGCAAA AAAGTCAGCC ACAGATGAGT
    GGAATAGAAG AGGAAGATAT TCCCCCTAAT CAAGGAGAAC GAAAGCTTTC ATTAGTTCCT
    GAATCAGAAC AGGGAGAAGC CAGCCTGCCT AGAAGCAATG TTCTTAATAC AGGTCCTACA
    TTTCAAGGCC GCAGGAGACA GTCTGTACTA AATCTAATGA CTCGCACCTC TGTTTCTCAA
    GGTTCCAATG CCTTTGCAAC AAGAAATACA TCTGTTCGTA AAATGTCTGT AGGTTCTTTC
    TCCAACTCAT CTTTTGAAGT GGATATATAT AACAGACGAC TTTCACAAGA CAGTATTTTG
    GAAGTTAGTG AAGAAATCAA TGAAGAAGAT TTGAAGGAAT GTTTCCTGGA TGATACAGAC
    AGCCCATCAC CAACTACCAC ATGGAACACA TATATAAGAT TCCTTACAGC TCACAAGAAT
    TTCATTTTTA TTCTTGTATT TTGTATAGTG ATATTTTTAG CTGAGGTCAT TGCATCGTCA
    GCTGGGCTCT GGATTATTAA ACGAAATGCT CCTGTTAGCA ACATGACAAG TAATGAGAAT
    ATATCTGAAG CATCAGACAT CCTTTCTGTC ATTGTGACAC ACACTAGCTT TTACTATGTG
    TTTTACATTT ACGTTGGTGT GGCTGACTCC TTACTGGCTC TGGGAATATT CAGAGGTTTG
    CCTCTCGTAC ACAGTCTTAT ATCAGTCTCG AGGGTATTAC ACAAAAAAAT GCTGCATTCT
    ATTCTACATG CCCCAATGTC AACCTTCAAT ACAATGAGAG CTGGTCGGAT ACTTAACAGA
    TTTTCAAAAG ATACAGCAAT ATTGGATGAC ATTCTACCTC TGTCAATATT TGACTTCACA
    CAGCTTGTGC TTATTGTGAT TGGAGCAATA ACAGTGGTTT CATTACTTGA ACCTTACATT
    TTCCTGGCAA CTGTGCCTGT AATTGCTGCT TTTATTCTCT TGAGATCATA CTTTCTTCAC
    ACATCTCAGC AACTAAAACA GTTAGAATCT GAAGCCCGCA GTCCGATTTT TGCTCACCTT
    ATAACAAGTT TGAAGGGTTT ATGGACATTG AGAGCCTTCG GGAGACAACC ATATTTTGAA
    ACCCTTTTTC ACAAAGCACT GAATTTACAT ACTGCAAACT GGTTCCTCTA TCTGTCCACT
    CTACGCTGGT TCCAAATGAC AATAGAAATG ATTTTTGTGA TATTCTTTAT CGCTGTTTCA
    TTCATCTCCA TCGCAACATC AGGTGCTGGG GAAGAAAAAG TTGGAATTGT TCTGACATTG
    GCAATGAATA TCATGAATAC ATTACAGTGG GCTGTAAATG CTAGCATTGA TGTGGATAGC
    TTGATGCGTT CTGTCAGCAG AATTTTCAGG TTCATTGACA TGCCAAGAGA AGAGTTGATA
    CATGACAATA ACCACAAAGA TGACCAACTT TCAGAGGTTC TGATATATGA AAATGATTAC
    ATCAAGAAAA ACCAAGTCTG GCCATCTGGA GGTCAAATGA CAGTGAAAAA TCTTTCTGCA
    AAATATAAAG ATGGAGGCCA CACTGTGTTG GAAAATATAT CCTTCTCTTT AAACCCTGGA
    CAAAGGGTAG GCTTATTAGG AAGAACTGGT TCAGGTAAAA GCACCCTGTT GTCTGCCTTT
    TTGAGATTAT TATCAACCCA AGGAGACATT CAAATCGATG GGGTATCCTG GCAAACAATT
    CCACTTCAGA AATGGAGAAA GGCATTTGGA GTAGTTCCAC AGAAAGTATT TATATTTTCT
    GGAACTATTC GAAAAAACCT TGATCCCTAT GGAAAATGGA GTGATGAGGA ACTTTTGAAA
    GTCACAGAAG AGGTTGGTTT AAAGTCAATA ATTGATCAGT TTCCGGGACA GTTGGATTTT
    GTGCTTCTGG ATGGGGGTTG TGTCCTGAGC CATGGCCACA AGCAACTCGT ATGTTTGGCT
    AGGTCGGTTC TCAGCAAAGC AAAAATCTTA TTACTCGATG AGCCTAGTGC ACATTTGGAT
    CCAATAACAT TCCAAATAAT TAGGAAAACA CTAAAACATG CATTTGCAAA TTGCACAGTA
    ATTTTGTCAG AACACAGACT AGAGGCAATG TTGGAATGCC AGCACTTTCT GGTGATTGAA
    GACAATACAG TACGACAGTA TGACTCTATT CAAAAGCTTA TGAATGAGAA GAGTTTCTTC
    AAACAAGCCA TAAGCCAATC TGATCGCTTG AAACTATTCC CATTGCATCG TAGAAATTCA
    AGTAAACGAA AATCCAGACC CAAAATTTCT GCCTTACAAG AAGAGACAGA AGAGGAGGTT
    CAGGAAACAA GACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa54777
    Clone ID Related Accession (Same CDS sequence) XM_012959952.3
    Accession Version XM_012959952.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cystic fibrosis transmembrane conductance regulator isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTTTATA GCTGGACCAA GCCAATTTTG TGGAAAGGCT ATAGGCAACG GCTGGAACTG 
    TCAGACATTT ACCAGATTCA CCCAGGGGAC TCTGCAGATA ACCTTTCAGA AAGGCTAGAA
    AGAGAATGGG ACAGGGAGGT GGCAACATCA AAGAAAAACC CCAAATTAAT TAATGCACTG
    AAACGATGTT TTTTCTGGAA ATTTCTGTTC TATGGTATCT TATTATACCT TGGGGAAGTA
    ACTAAAGCTG TTCAACCTCT TCTTCTTGGA AGAATCATTG CTTCTTATGA CCGCGACAAT
    GAGCATGAAC GATCGATTGC TTATTACCTG GCAATTGGCC TATGTCTACT TTTTGTAGTG
    AGAATGCTTC TTTTACACCC TGCCATTTTT GGTCTACACC ATATTGGAAT GCAAATGAGA
    ATAGCAATGT TTAGTTTAAT TTACAAAAAG ACTTTAAAAC TTTCAAGCAG AGTTCTCGAT
    AAAATAAGCA CTGGACAGCT TGTAAGTCTT TTATCAAACA ACCTCAATAA ATTTGATGAG
    GGACTTGCTC TGGCTCATTT TGTTTGGATT GCACCTTTGC AAGTGCTGCT GTTGATGGGA
    CTGCTGTGGG ACTTACTTCA GGCCTCAGCA TTTTGTGGGC TTGGCTTTCT TATCATCCTT
    GCATTGTTCC AAGCATGGCT TGGCCGTATG ATGATGAAAT ATAGGGATAA GAGGGCAGGA
    AAGATCAATG AACGACTTGT GATAACATCT GAAATCATAG AGAACATCCA GTCAGTAAAG
    GCCTACTGTT GGGAGAGTGC AATGGAAAAA ATAATTGAAA CCATAAGAGA GACTGAACTG
    AAGCTTACTC GAAAAGCAGC ATATGTGCGT TACTTCAACA GCTCAGCATT CTTTTTCTCT
    GGATTTTTTG TGGTGTTTCT TTGTATTGTG CCTCATCTAC TACTTGATGG CATTTCACTC
    CGAAAAATAT TTACTACCAT TTCATTTAGC ATAGTCCTGC GAATGGCAGT CACAAGGCAG
    TTCCCCTGGG CTGTACAGAC ATGGTATGAT TCCCTTGGAG TGATAAATAA AATACAGGAA
    TTCCTGCAAA AGGAAGAATA TAAATCACTG GAATATAATT TAACAACCAC AGAAGTTGCA
    ATGGAAAATG TTTCAGCATC TTGGGATGAG GGCATTGGAG AATTCTTTGA AAAAGCAAAG
    CAAGAAGTAA ATGGAGGGAA TATTTCTAAT GAGGATCCAA GTCCGTTCTT TAGTAACTTT
    TCCCTGCATG TTGCTCCAGT CCTCAGAAAC ATCAATTTTA AGATTGAAAA AGGGCAGCTT
    TTGGCAATTG CTGGATCTAC AGGGGCTGGA AAGACTTCTC TATTGATGAT GATCATGGGA
    GAACTGGAGC CGTCAGCTGG TAAAATAAAG CATAGCGGAA GGATATCTTT TTCTCCTCAA
    GTATCTTGGA TCATGCCGGG GACCATCAAA GAGAATATAA TATTTGGCGT TTCCTATGAC
    CAGTACAGAT ACCTGAGTGT TATAAAAGCT TGTCAGCTGG AAGAGGACAT TTCAAAATTT
    CCAGAAAAAG ATAATACTGT GTTAGGAGAA GGGGGAATAA CACTAAGTGG AGGCCAGCGT
    GCAAGGATTT CACTTGCAAG AGCCGTATAC AAAGATGCTG ACCTCTACTT GATGGATTCT
    CCATTCAGTT ATTTAGACTT GTTTACAGAA AAAGAAATAT TTGAAAGTTG TGTTTGCAAG
    CTAATGGCTA ATAAAACAAG GATTTTAGTT ACATCTAAAG TTGAACAGCT TAAAAAAGCT
    GACAAAGTGC TGATTTTGCA TGAAGGAAGC TGTTATTTCT ATGGAACATT TTCTGAACTT
    CAAGATCAAC GTCCTGAGTT CAGCTCTCAT CTGACCGGAT TTGATCACTT TAATGCAGAA
    AGAAGAAACT CTATAATAAC TGAAACCCTG AGAAGATGCT CCATTGATAG CGATCCAACA
    GCTGTTAGGA ATGAAGTCAA GAATAAGTCA TTTAAGCAGG TTGCAGATTT CAGTGAAAAA
    AGAAAGAACT CTGTTATTAA TCCTCGGAAA TCCAGCAGAA AATTTTCTAT CATGCAAAAA
    AGTCAGCCAC AGATGAGTGG AATAGAAGAG GAAGATATTC CCCCTAATCA AGGAGAACGA
    AAGCTTTCAT TAGTTCCTGA ATCAGAACAG GGAGAAGCCA GCCTGCCTAG AAGCAATGTT
    CTTAATACAG GTCCTACATT TCAAGGCCGC AGGAGACAGT CTGTACTAAA TCTAATGACT
    CGCACCTCTG TTTCTCAAGG TTCCAATGCC TTTGCAACAA GAAATACATC TGTTCGTAAA
    ATGTCTGTAG GTTCTTTCTC CAACTCATCT TTTGAAGTGG ATATATATAA CAGACGACTT
    TCACAAGACA GTATTTTGGA AGTTAGTGAA GAAATCAATG AAGAAGATTT GAAGGAATGT
    TTCCTGGATG ATACAGACAG CCCATCACCA ACTACCACAT GGAACACATA TATAAGATTC
    CTTACAGCTC ACAAGAATTT CATTTTTATT CTTGTATTTT GTATAGTGAT ATTTTTAGCT
    GAGGTCATTG CATCGTCAGC TGGGCTCTGG ATTATTAAAC GAAATGCTCC TGTTAGCAAC
    ATGACAAGTA ATGAGAATAT ATCTGAAGCA TCAGACATCC TTTCTGTCAT TGTGACACAC
    ACTAGCTTTT ACTATGTGTT TTACATTTAC GTTGGTGTGG CTGACTCCTT ACTGGCTCTG
    GGAATATTCA GAGGTTTGCC TCTCGTACAC AGTCTTATAT CAGTCTCGAG GGTATTACAC
    AAAAAAATGC TGCATTCTAT TCTACATGCC CCAATGTCAA CCTTCAATAC AATGAGAGCT
    GGTCGGATAC TTAACAGATT TTCAAAAGAT ACAGCAATAT TGGATGACAT TCTACCTCTG
    TCAATATTTG ACTTCACACA GCTTGTGCTT ATTGTGATTG GAGCAATAAC AGTGGTTTCA
    TTACTTGAAC CTTACATTTT CCTGGCAACT GTGCCTGTAA TTGCTGCTTT TATTCTCTTG
    AGATCATACT TTCTTCACAC ATCTCAGCAA CTAAAACAGT TAGAATCTGA AGCCCGCAGT
    CCGATTTTTG CTCACCTTAT AACAAGTTTG AAGGGTTTAT GGACATTGAG AGCCTTCGGG
    AGACAACCAT ATTTTGAAAC CCTTTTTCAC AAAGCACTGA ATTTACATAC TGCAAACTGG
    TTCCTCTATC TGTCCACTCT ACGCTGGTTC CAAATGACAA TAGAAATGAT TTTTGTGATA
    TTCTTTATCG CTGTTTCATT CATCTCCATC GCAACATCAG GTGCTGGGGA AGAAAAAGTT
    GGAATTGTTC TGACATTGGC AATGAATATC ATGAATACAT TACAGTGGGC TGTAAATGCT
    AGCATTGATG TGGATAGCTT GATGCGTTCT GTCAGCAGAA TTTTCAGGTT CATTGACATG
    CCAAGAGAAG AGTTGATACA TGACAATAAC CACAAAGATG ACCAACTTTC AGAGGTTCTG
    ATATATGAAA ATGATTACAT CAAGAAAAAC CAAGTCTGGC CATCTGGAGG TCAAATGACA
    GTGAAAAATC TTTCTGCAAA ATATAAAGAT GGAGGCCACA CTGTGTTGGA AAATATATCC
    TTCTCTTTAA ACCCTGGACA AAGGGTAGGC TTATTAGGAA GAACTGGTTC AGGTAAAAGC
    ACCCTGTTGT CTGCCTTTTT GAGATTATTA TCAACCCAAG GAGACATTCA AATCGATGGG
    GTATCCTGGC AAACAATTCC ACTTCAGAAA TGGAGAAAGG CATTTGGAGT AGTTCCACAG
    AAAGTATTTA TATTTTCTGG AACTATTCGA AAAAACCTTG ATCCCTATGG AAAATGGAGT
    GATGAGGAAC TTTTGAAAGT CACAGAAGAG GTTGGTTTAA AGTCAATAAT TGATCAGTTT
    CCGGGACAGT TGGATTTTGT GCTTCTGGAT GGGGGTTGTG TCCTGAGCCA TGGCCACAAG
    CAACTCGTAT GTTTGGCTAG GTCGGTTCTC AGCAAAGCAA AAATCTTATT ACTCGATGAG
    CCTAGTGCAC ATTTGGATCC AATAACATTC CAAATAATTA GGAAAACACT AAAACATGCA
    TTTGCAAATT GCACAGTAAT TTTGTCAGAA CACAGACTAG AGGCAATGTT GGAATGCCAG
    CACTTTCTGG TGATTGAAGA CAATACAGTA CGACAGTATG ACTCTATTCA AAAGCTTATG
    AATGAGAAGA GTTTCTTCAA ACAAGCCATA AGCCAATCTG ATCGCTTGAA ACTATTCCCA
    TTGCATCGTA GAAATTCAAG TAAACGAAAA TCCAGACCCA AAATTTCTGC CTTACAAGAA
    GAGACAGAAG AGGAGGTTCA GGAAACAAGA CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815406.2
    CDS1..4416
    Translation

    Target ORF information:

    RefSeq Version XM_012959952.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis cystic fibrosis transmembrane conductance regulator (cftr), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959952.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTTTATA GCTGGACCAA GCCAATTTTG TGGAAAGGCT ATAGGCAACG GCTGGAACTG 
    TCAGACATTT ACCAGATTCA CCCAGGGGAC TCTGCAGATA ACCTTTCAGA AAGGCTAGAA
    AGAGAATGGG ACAGGGAGGT GGCAACATCA AAGAAAAACC CCAAATTAAT TAATGCACTG
    AAACGATGTT TTTTCTGGAA ATTTCTGTTC TATGGTATCT TATTATACCT TGGGGAAGTA
    ACTAAAGCTG TTCAACCTCT TCTTCTTGGA AGAATCATTG CTTCTTATGA CCGCGACAAT
    GAGCATGAAC GATCGATTGC TTATTACCTG GCAATTGGCC TATGTCTACT TTTTGTAGTG
    AGAATGCTTC TTTTACACCC TGCCATTTTT GGTCTACACC ATATTGGAAT GCAAATGAGA
    ATAGCAATGT TTAGTTTAAT TTACAAAAAG ACTTTAAAAC TTTCAAGCAG AGTTCTCGAT
    AAAATAAGCA CTGGACAGCT TGTAAGTCTT TTATCAAACA ACCTCAATAA ATTTGATGAG
    GGACTTGCTC TGGCTCATTT TGTTTGGATT GCACCTTTGC AAGTGCTGCT GTTGATGGGA
    CTGCTGTGGG ACTTACTTCA GGCCTCAGCA TTTTGTGGGC TTGGCTTTCT TATCATCCTT
    GCATTGTTCC AAGCATGGCT TGGCCGTATG ATGATGAAAT ATAGGGATAA GAGGGCAGGA
    AAGATCAATG AACGACTTGT GATAACATCT GAAATCATAG AGAACATCCA GTCAGTAAAG
    GCCTACTGTT GGGAGAGTGC AATGGAAAAA ATAATTGAAA CCATAAGAGA GACTGAACTG
    AAGCTTACTC GAAAAGCAGC ATATGTGCGT TACTTCAACA GCTCAGCATT CTTTTTCTCT
    GGATTTTTTG TGGTGTTTCT TTGTATTGTG CCTCATCTAC TACTTGATGG CATTTCACTC
    CGAAAAATAT TTACTACCAT TTCATTTAGC ATAGTCCTGC GAATGGCAGT CACAAGGCAG
    TTCCCCTGGG CTGTACAGAC ATGGTATGAT TCCCTTGGAG TGATAAATAA AATACAGGAA
    TTCCTGCAAA AGGAAGAATA TAAATCACTG GAATATAATT TAACAACCAC AGAAGTTGCA
    ATGGAAAATG TTTCAGCATC TTGGGATGAG GGCATTGGAG AATTCTTTGA AAAAGCAAAG
    CAAGAAGTAA ATGGAGGGAA TATTTCTAAT GAGGATCCAA GTCCGTTCTT TAGTAACTTT
    TCCCTGCATG TTGCTCCAGT CCTCAGAAAC ATCAATTTTA AGATTGAAAA AGGGCAGCTT
    TTGGCAATTG CTGGATCTAC AGGGGCTGGA AAGACTTCTC TATTGATGAT GATCATGGGA
    GAACTGGAGC CGTCAGCTGG TAAAATAAAG CATAGCGGAA GGATATCTTT TTCTCCTCAA
    GTATCTTGGA TCATGCCGGG GACCATCAAA GAGAATATAA TATTTGGCGT TTCCTATGAC
    CAGTACAGAT ACCTGAGTGT TATAAAAGCT TGTCAGCTGG AAGAGGACAT TTCAAAATTT
    CCAGAAAAAG ATAATACTGT GTTAGGAGAA GGGGGAATAA CACTAAGTGG AGGCCAGCGT
    GCAAGGATTT CACTTGCAAG AGCCGTATAC AAAGATGCTG ACCTCTACTT GATGGATTCT
    CCATTCAGTT ATTTAGACTT GTTTACAGAA AAAGAAATAT TTGAAAGTTG TGTTTGCAAG
    CTAATGGCTA ATAAAACAAG GATTTTAGTT ACATCTAAAG TTGAACAGCT TAAAAAAGCT
    GACAAAGTGC TGATTTTGCA TGAAGGAAGC TGTTATTTCT ATGGAACATT TTCTGAACTT
    CAAGATCAAC GTCCTGAGTT CAGCTCTCAT CTGACCGGAT TTGATCACTT TAATGCAGAA
    AGAAGAAACT CTATAATAAC TGAAACCCTG AGAAGATGCT CCATTGATAG CGATCCAACA
    GCTGTTAGGA ATGAAGTCAA GAATAAGTCA TTTAAGCAGG TTGCAGATTT CAGTGAAAAA
    AGAAAGAACT CTGTTATTAA TCCTCGGAAA TCCAGCAGAA AATTTTCTAT CATGCAAAAA
    AGTCAGCCAC AGATGAGTGG AATAGAAGAG GAAGATATTC CCCCTAATCA AGGAGAACGA
    AAGCTTTCAT TAGTTCCTGA ATCAGAACAG GGAGAAGCCA GCCTGCCTAG AAGCAATGTT
    CTTAATACAG GTCCTACATT TCAAGGCCGC AGGAGACAGT CTGTACTAAA TCTAATGACT
    CGCACCTCTG TTTCTCAAGG TTCCAATGCC TTTGCAACAA GAAATACATC TGTTCGTAAA
    ATGTCTGTAG GTTCTTTCTC CAACTCATCT TTTGAAGTGG ATATATATAA CAGACGACTT
    TCACAAGACA GTATTTTGGA AGTTAGTGAA GAAATCAATG AAGAAGATTT GAAGGAATGT
    TTCCTGGATG ATACAGACAG CCCATCACCA ACTACCACAT GGAACACATA TATAAGATTC
    CTTACAGCTC ACAAGAATTT CATTTTTATT CTTGTATTTT GTATAGTGAT ATTTTTAGCT
    GAGGTCATTG CATCGTCAGC TGGGCTCTGG ATTATTAAAC GAAATGCTCC TGTTAGCAAC
    ATGACAAGTA ATGAGAATAT ATCTGAAGCA TCAGACATCC TTTCTGTCAT TGTGACACAC
    ACTAGCTTTT ACTATGTGTT TTACATTTAC GTTGGTGTGG CTGACTCCTT ACTGGCTCTG
    GGAATATTCA GAGGTTTGCC TCTCGTACAC AGTCTTATAT CAGTCTCGAG GGTATTACAC
    AAAAAAATGC TGCATTCTAT TCTACATGCC CCAATGTCAA CCTTCAATAC AATGAGAGCT
    GGTCGGATAC TTAACAGATT TTCAAAAGAT ACAGCAATAT TGGATGACAT TCTACCTCTG
    TCAATATTTG ACTTCACACA GCTTGTGCTT ATTGTGATTG GAGCAATAAC AGTGGTTTCA
    TTACTTGAAC CTTACATTTT CCTGGCAACT GTGCCTGTAA TTGCTGCTTT TATTCTCTTG
    AGATCATACT TTCTTCACAC ATCTCAGCAA CTAAAACAGT TAGAATCTGA AGCCCGCAGT
    CCGATTTTTG CTCACCTTAT AACAAGTTTG AAGGGTTTAT GGACATTGAG AGCCTTCGGG
    AGACAACCAT ATTTTGAAAC CCTTTTTCAC AAAGCACTGA ATTTACATAC TGCAAACTGG
    TTCCTCTATC TGTCCACTCT ACGCTGGTTC CAAATGACAA TAGAAATGAT TTTTGTGATA
    TTCTTTATCG CTGTTTCATT CATCTCCATC GCAACATCAG GTGCTGGGGA AGAAAAAGTT
    GGAATTGTTC TGACATTGGC AATGAATATC ATGAATACAT TACAGTGGGC TGTAAATGCT
    AGCATTGATG TGGATAGCTT GATGCGTTCT GTCAGCAGAA TTTTCAGGTT CATTGACATG
    CCAAGAGAAG AGTTGATACA TGACAATAAC CACAAAGATG ACCAACTTTC AGAGGTTCTG
    ATATATGAAA ATGATTACAT CAAGAAAAAC CAAGTCTGGC CATCTGGAGG TCAAATGACA
    GTGAAAAATC TTTCTGCAAA ATATAAAGAT GGAGGCCACA CTGTGTTGGA AAATATATCC
    TTCTCTTTAA ACCCTGGACA AAGGGTAGGC TTATTAGGAA GAACTGGTTC AGGTAAAAGC
    ACCCTGTTGT CTGCCTTTTT GAGATTATTA TCAACCCAAG GAGACATTCA AATCGATGGG
    GTATCCTGGC AAACAATTCC ACTTCAGAAA TGGAGAAAGG CATTTGGAGT AGTTCCACAG
    AAAGTATTTA TATTTTCTGG AACTATTCGA AAAAACCTTG ATCCCTATGG AAAATGGAGT
    GATGAGGAAC TTTTGAAAGT CACAGAAGAG GTTGGTTTAA AGTCAATAAT TGATCAGTTT
    CCGGGACAGT TGGATTTTGT GCTTCTGGAT GGGGGTTGTG TCCTGAGCCA TGGCCACAAG
    CAACTCGTAT GTTTGGCTAG GTCGGTTCTC AGCAAAGCAA AAATCTTATT ACTCGATGAG
    CCTAGTGCAC ATTTGGATCC AATAACATTC CAAATAATTA GGAAAACACT AAAACATGCA
    TTTGCAAATT GCACAGTAAT TTTGTCAGAA CACAGACTAG AGGCAATGTT GGAATGCCAG
    CACTTTCTGG TGATTGAAGA CAATACAGTA CGACAGTATG ACTCTATTCA AAAGCTTATG
    AATGAGAAGA GTTTCTTCAA ACAAGCCATA AGCCAATCTG ATCGCTTGAA ACTATTCCCA
    TTGCATCGTA GAAATTCAAG TAAACGAAAA TCCAGACCCA AAATTTCTGC CTTACAAGAA
    GAGACAGAAG AGGAGGTTCA GGAAACAAGA CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33528
    Clone ID Related Accession (Same CDS sequence) XM_012959952.2
    Accession Version XM_012959952.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product cystic fibrosis transmembrane conductance regulator
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTTTATA GCTGGACCAA GCCAATTTTG TGGAAAGGCT ATAGGCAACG GCTGGAACTG 
    TCAGACATTT ACCAGATTCA CCCAGGGGAC TCTGCAGATA ACCTTTCAGA AAGGCTAGAA
    AGAGAATGGG ACAGGGAGGT GGCAACATCA AAGAAAAACC CCAAATTAAT TAATGCACTG
    AAACGATGTT TTTTCTGGAA ATTTCTGTTC TATGGTATCT TATTATACCT TGGGGAAGTA
    ACTAAAGCTG TTCAACCTCT TCTTCTTGGA AGAATCATTG CTTCTTATGA CCGCGACAAT
    GAGCATGAAC GATCGATTGC TTATTACCTG GCAATTGGCC TATGTCTACT TTTTGTAGTG
    AGAATGCTTC TTTTACACCC TGCCATTTTT GGTCTACACC ATATTGGAAT GCAAATGAGA
    ATAGCAATGT TTAGTTTAAT TTACAAAAAG ACTTTAAAAC TTTCAAGCAG AGTTCTCGAT
    AAAATAAGCA CTGGACAGCT TGTAAGTCTT TTATCAAACA ACCTCAATAA ATTTGATGAG
    GGACTTGCTC TGGCTCATTT TGTTTGGATT GCACCTTTGC AAGTGCTGCT GTTGATGGGA
    CTGCTGTGGG ACTTACTTCA GGCCTCAGCA TTTTGTGGGC TTGGCTTTCT TATCATCCTT
    GCATTGTTCC AAGCATGGCT TGGCCGTATG ATGATGAAAT ATAGGGATAA GAGGGCAGGA
    AAGATCAATG AACGACTTGT GATAACATCT GAAATCATAG AGAACATCCA GTCAGTAAAG
    GCCTACTGTT GGGAGAGTGC AATGGAAAAA ATAATTGAAA CCATAAGAGA GACTGAACTG
    AAGCTTACTC GAAAAGCAGC ATATGTGCGT TACTTCAACA GCTCAGCATT CTTTTTCTCT
    GGATTTTTTG TGGTGTTTCT TTGTATTGTG CCTCATCTAC TACTTGATGG CATTTCACTC
    CGAAAAATAT TTACTACCAT TTCATTTAGC ATAGTCCTGC GAATGGCAGT CACAAGGCAG
    TTCCCCTGGG CTGTACAGAC ATGGTATGAT TCCCTTGGAG TGATAAATAA AATACAGGAA
    TTCCTGCAAA AGGAAGAATA TAAATCACTG GAATATAATT TAACAACCAC AGAAGTTGCA
    ATGGAAAATG TTTCAGCATC TTGGGATGAG GGCATTGGAG AATTCTTTGA AAAAGCAAAG
    CAAGAAGTAA ATGGAGGGAA TATTTCTAAT GAGGATCCAA GTCCGTTCTT TAGTAACTTT
    TCCCTGCATG TTGCTCCAGT CCTCAGAAAC ATCAATTTTA AGATTGAAAA AGGGCAGCTT
    TTGGCAATTG CTGGATCTAC AGGGGCTGGA AAGACTTCTC TATTGATGAT GATCATGGGA
    GAACTGGAGC CGTCAGCTGG TAAAATAAAG CATAGCGGAA GGATATCTTT TTCTCCTCAA
    GTATCTTGGA TCATGCCGGG GACCATCAAA GAGAATATAA TATTTGGCGT TTCCTATGAC
    CAGTACAGAT ACCTGAGTGT TATAAAAGCT TGTCAGCTGG AAGAGGACAT TTCAAAATTT
    CCAGAAAAAG ATAATACTGT GTTAGGAGAA GGGGGAATAA CACTAAGTGG AGGCCAGCGT
    GCAAGGATTT CACTTGCAAG AGCCGTATAC AAAGATGCTG ACCTCTACTT GATGGATTCT
    CCATTCAGTT ATTTAGACTT GTTTACAGAA AAAGAAATAT TTGAAAGTTG TGTTTGCAAG
    CTAATGGCTA ATAAAACAAG GATTTTAGTT ACATCTAAAG TTGAACAGCT TAAAAAAGCT
    GACAAAGTGC TGATTTTGCA TGAAGGAAGC TGTTATTTCT ATGGAACATT TTCTGAACTT
    CAAGATCAAC GTCCTGAGTT CAGCTCTCAT CTGACCGGAT TTGATCACTT TAATGCAGAA
    AGAAGAAACT CTATAATAAC TGAAACCCTG AGAAGATGCT CCATTGATAG CGATCCAACA
    GCTGTTAGGA ATGAAGTCAA GAATAAGTCA TTTAAGCAGG TTGCAGATTT CAGTGAAAAA
    AGAAAGAACT CTGTTATTAA TCCTCGGAAA TCCAGCAGAA AATTTTCTAT CATGCAAAAA
    AGTCAGCCAC AGATGAGTGG AATAGAAGAG GAAGATATTC CCCCTAATCA AGGAGAACGA
    AAGCTTTCAT TAGTTCCTGA ATCAGAACAG GGAGAAGCCA GCCTGCCTAG AAGCAATGTT
    CTTAATACAG GTCCTACATT TCAAGGCCGC AGGAGACAGT CTGTACTAAA TCTAATGACT
    CGCACCTCGG TTTCTCAAGG TTCCAATGCC TTTGCAACAA GAAATACATC TGTTCGTAAA
    ATGTCTGTAG GTTCTTTCTC CAACTCATCT TTTGAAGTGG ATATATATAA CAGACGACTT
    TCACAAGACA GTATTTTGGA AGTTAGTGAA GAAATCAATG AAGAAGATTT GAAGGAATGT
    TTCCTGGATG ATACAGACAG CCCATCACCG ACTACCACAT GGAACACATA TATAAGATTC
    CTTACAGCTC ACAAGAATTT CATTTTTATT CTAGTATTTT GTATAGGGAT ATTTTTAGCT
    GAGGTCATTG CATCGTCAGC TGGGCTCTGG ATTATTAAAC GAAATGCTCC TGTTAGCAAC
    ATGACAAGTA ATGAGAATAT ATCTGAAGCA TCAGACATCC TTTCTGTCAT TGTGACACAC
    ACTAGCTTTT ACTATGTGTT TTACATTTAC GTTGGTGTGG CTGACTCCTT ACTGGCTCTG
    GGAATATTCA GAGGTTTGCC TCTCGTACAC AGTCTTATAT CAGTCTCGAG GGTATTACAC
    AAAAAAATGC TGCATTCTAT TCTACATGCC CCAATGTCAA CCTTCAATAC AATGAGAGCT
    GGTCGGATAC TTAACAGATT TTCAAAAGAT ACAGCAATAT TGGATGACAT TCTACCGCTG
    TCAATATTTG ACTTCACACA GCTTGTGCTT ATTGTGATTG GAGCAATAAC AGTGGTTTCA
    TTACTTGAAC CTTACATTTT CCTGGCAACT GTGCCTGTAA TTGCTGCTTT TATTCTCTTG
    AGATCATACT TTCTTCACAC ATCTCAGCAA CTAAAACAGT TAGAATCTGA AGCCCGCAGT
    CCGATTTTTG CTCACCTTAT AACAAGTTTG AAGGGTTTAT GGACATTGAG AGCCTTCGGG
    AGACAACCAT ATTTTGAAAC CCTTTTTCAC AAAGCACTGA ATTTACATAC TGCAAACTGG
    TTCCTTTATC TGTCCACTCT ACGCTGGTTC CAAATGACAA TAGAAATGAT TTTTGTGATA
    TTCTTTATCG CTGTTTCATT CATCTCCATC GCAACATCAG GTGCTGGGGA AGAAAAAGTT
    GGAATTGTTC TGACATTGGC AATGAATATC ATGAATACAT TACAGTGGGC TGTAAATGCT
    AGCATTGATG TGGATAGCTT GATGCGTTCT GTCAGCAGAA TTTTCAGGTT CATTGACATG
    CCAAGAGAAG AGTTGATACA TGACAATAAC CACAAAGATG ACCAACTTTC AGAGGTTCTG
    ATATATGAAA ATGATTACAT CAAGAAAAAC CAAGTCTGGC CATCTGGAGG TCAAATGACA
    GTGAAAAATC TTTCTGCAAA ATATAAAGAT GGAGGCCACA CTGTGTTGGA AAATATATCC
    TTCTCTTTAA ACCCTGGACA AAGGGTAGGC TTATTAGGAA GAACTGGTTC AGGTAAAAGC
    ACCCTGTTGT CTGCCTTTTT GAGATTATTA TCAACCCAAG GAGACATTCA AATCGATGGG
    GTATCCTGGC AAACAATTCC ACTTCAGAAA TGGAGAAAGG CATTTGGAGT AGTTCCACAG
    AAAGTATTTA TATTTTCTGG AACTATTCGA AAAAACCTTG ATCCCTATGG AAAATGGAGT
    GATGAGGAAC TTTTGAAAGT CACAGAAGAG GTTGGTTTAA AGTCAATAAT TGATCAGTTT
    CCGGGACAGT TGGATTTTGT GCTTCTGGAT GGGGGTTGTG TCCTGAGCCA TGGCCACAAG
    CAACTCGTAT GTTTGGCTAG GTCGGTTCTC AGCAAAGCAA AAATCTTATT ACTCGATGAG
    CCTAGTGCAC ATTTGGATCC AATAACATTC CAAATAATTA GGAAAACACT AAAACATGCA
    TTTGCAAATT GCACAGTAAT TTTGTCAGAA CACAGACTAG AGGCAATGTT GGAATGCCAG
    CACTTTCTGG TGATTGAAGA CAATACAGTA CGACAGTATG ACTCTATTCA AAAGCTTATG
    AATGAGAAGA GTTTCTTCAA ACAAGCCATA AGCCAATCTG ATCGCTTGAA ACTATTCCCA
    TTGCATCGTA GAAATTCAAG TAAACGAAAA TCCAGACCCA AAATTTCTGC CTTACAAGAA
    GAGACAGAAG AGGAGGTTCA GGAAACAAGA CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012815406.1
    CDS1..4416
    Translation

    Target ORF information:

    RefSeq Version XM_012959952.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis cystic fibrosis transmembrane conductance regulator (cftr), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012959952.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTTTATA GCTGGACCAA GCCAATTTTG TGGAAAGGCT ATAGGCAACG GCTGGAACTG 
    TCAGACATTT ACCAGATTCA CCCAGGGGAC TCTGCAGATA ACCTTTCAGA AAGGCTAGAA
    AGAGAATGGG ACAGGGAGGT GGCAACATCA AAGAAAAACC CCAAATTAAT TAATGCACTG
    AAACGATGTT TTTTCTGGAA ATTTCTGTTC TATGGTATCT TATTATACCT TGGGGAAGTA
    ACTAAAGCTG TTCAACCTCT TCTTCTTGGA AGAATCATTG CTTCTTATGA CCGCGACAAT
    GAGCATGAAC GATCGATTGC TTATTACCTG GCAATTGGCC TATGTCTACT TTTTGTAGTG
    AGAATGCTTC TTTTACACCC TGCCATTTTT GGTCTACACC ATATTGGAAT GCAAATGAGA
    ATAGCAATGT TTAGTTTAAT TTACAAAAAG ACTTTAAAAC TTTCAAGCAG AGTTCTCGAT
    AAAATAAGCA CTGGACAGCT TGTAAGTCTT TTATCAAACA ACCTCAATAA ATTTGATGAG
    GGACTTGCTC TGGCTCATTT TGTTTGGATT GCACCTTTGC AAGTGCTGCT GTTGATGGGA
    CTGCTGTGGG ACTTACTTCA GGCCTCAGCA TTTTGTGGGC TTGGCTTTCT TATCATCCTT
    GCATTGTTCC AAGCATGGCT TGGCCGTATG ATGATGAAAT ATAGGGATAA GAGGGCAGGA
    AAGATCAATG AACGACTTGT GATAACATCT GAAATCATAG AGAACATCCA GTCAGTAAAG
    GCCTACTGTT GGGAGAGTGC AATGGAAAAA ATAATTGAAA CCATAAGAGA GACTGAACTG
    AAGCTTACTC GAAAAGCAGC ATATGTGCGT TACTTCAACA GCTCAGCATT CTTTTTCTCT
    GGATTTTTTG TGGTGTTTCT TTGTATTGTG CCTCATCTAC TACTTGATGG CATTTCACTC
    CGAAAAATAT TTACTACCAT TTCATTTAGC ATAGTCCTGC GAATGGCAGT CACAAGGCAG
    TTCCCCTGGG CTGTACAGAC ATGGTATGAT TCCCTTGGAG TGATAAATAA AATACAGGAA
    TTCCTGCAAA AGGAAGAATA TAAATCACTG GAATATAATT TAACAACCAC AGAAGTTGCA
    ATGGAAAATG TTTCAGCATC TTGGGATGAG GGCATTGGAG AATTCTTTGA AAAAGCAAAG
    CAAGAAGTAA ATGGAGGGAA TATTTCTAAT GAGGATCCAA GTCCGTTCTT TAGTAACTTT
    TCCCTGCATG TTGCTCCAGT CCTCAGAAAC ATCAATTTTA AGATTGAAAA AGGGCAGCTT
    TTGGCAATTG CTGGATCTAC AGGGGCTGGA AAGACTTCTC TATTGATGAT GATCATGGGA
    GAACTGGAGC CGTCAGCTGG TAAAATAAAG CATAGCGGAA GGATATCTTT TTCTCCTCAA
    GTATCTTGGA TCATGCCGGG GACCATCAAA GAGAATATAA TATTTGGCGT TTCCTATGAC
    CAGTACAGAT ACCTGAGTGT TATAAAAGCT TGTCAGCTGG AAGAGGACAT TTCAAAATTT
    CCAGAAAAAG ATAATACTGT GTTAGGAGAA GGGGGAATAA CACTAAGTGG AGGCCAGCGT
    GCAAGGATTT CACTTGCAAG AGCCGTATAC AAAGATGCTG ACCTCTACTT GATGGATTCT
    CCATTCAGTT ATTTAGACTT GTTTACAGAA AAAGAAATAT TTGAAAGTTG TGTTTGCAAG
    CTAATGGCTA ATAAAACAAG GATTTTAGTT ACATCTAAAG TTGAACAGCT TAAAAAAGCT
    GACAAAGTGC TGATTTTGCA TGAAGGAAGC TGTTATTTCT ATGGAACATT TTCTGAACTT
    CAAGATCAAC GTCCTGAGTT CAGCTCTCAT CTGACCGGAT TTGATCACTT TAATGCAGAA
    AGAAGAAACT CTATAATAAC TGAAACCCTG AGAAGATGCT CCATTGATAG CGATCCAACA
    GCTGTTAGGA ATGAAGTCAA GAATAAGTCA TTTAAGCAGG TTGCAGATTT CAGTGAAAAA
    AGAAAGAACT CTGTTATTAA TCCTCGGAAA TCCAGCAGAA AATTTTCTAT CATGCAAAAA
    AGTCAGCCAC AGATGAGTGG AATAGAAGAG GAAGATATTC CCCCTAATCA AGGAGAACGA
    AAGCTTTCAT TAGTTCCTGA ATCAGAACAG GGAGAAGCCA GCCTGCCTAG AAGCAATGTT
    CTTAATACAG GTCCTACATT TCAAGGCCGC AGGAGACAGT CTGTACTAAA TCTAATGACT
    CGCACCTCGG TTTCTCAAGG TTCCAATGCC TTTGCAACAA GAAATACATC TGTTCGTAAA
    ATGTCTGTAG GTTCTTTCTC CAACTCATCT TTTGAAGTGG ATATATATAA CAGACGACTT
    TCACAAGACA GTATTTTGGA AGTTAGTGAA GAAATCAATG AAGAAGATTT GAAGGAATGT
    TTCCTGGATG ATACAGACAG CCCATCACCG ACTACCACAT GGAACACATA TATAAGATTC
    CTTACAGCTC ACAAGAATTT CATTTTTATT CTAGTATTTT GTATAGGGAT ATTTTTAGCT
    GAGGTCATTG CATCGTCAGC TGGGCTCTGG ATTATTAAAC GAAATGCTCC TGTTAGCAAC
    ATGACAAGTA ATGAGAATAT ATCTGAAGCA TCAGACATCC TTTCTGTCAT TGTGACACAC
    ACTAGCTTTT ACTATGTGTT TTACATTTAC GTTGGTGTGG CTGACTCCTT ACTGGCTCTG
    GGAATATTCA GAGGTTTGCC TCTCGTACAC AGTCTTATAT CAGTCTCGAG GGTATTACAC
    AAAAAAATGC TGCATTCTAT TCTACATGCC CCAATGTCAA CCTTCAATAC AATGAGAGCT
    GGTCGGATAC TTAACAGATT TTCAAAAGAT ACAGCAATAT TGGATGACAT TCTACCGCTG
    TCAATATTTG ACTTCACACA GCTTGTGCTT ATTGTGATTG GAGCAATAAC AGTGGTTTCA
    TTACTTGAAC CTTACATTTT CCTGGCAACT GTGCCTGTAA TTGCTGCTTT TATTCTCTTG
    AGATCATACT TTCTTCACAC ATCTCAGCAA CTAAAACAGT TAGAATCTGA AGCCCGCAGT
    CCGATTTTTG CTCACCTTAT AACAAGTTTG AAGGGTTTAT GGACATTGAG AGCCTTCGGG
    AGACAACCAT ATTTTGAAAC CCTTTTTCAC AAAGCACTGA ATTTACATAC TGCAAACTGG
    TTCCTTTATC TGTCCACTCT ACGCTGGTTC CAAATGACAA TAGAAATGAT TTTTGTGATA
    TTCTTTATCG CTGTTTCATT CATCTCCATC GCAACATCAG GTGCTGGGGA AGAAAAAGTT
    GGAATTGTTC TGACATTGGC AATGAATATC ATGAATACAT TACAGTGGGC TGTAAATGCT
    AGCATTGATG TGGATAGCTT GATGCGTTCT GTCAGCAGAA TTTTCAGGTT CATTGACATG
    CCAAGAGAAG AGTTGATACA TGACAATAAC CACAAAGATG ACCAACTTTC AGAGGTTCTG
    ATATATGAAA ATGATTACAT CAAGAAAAAC CAAGTCTGGC CATCTGGAGG TCAAATGACA
    GTGAAAAATC TTTCTGCAAA ATATAAAGAT GGAGGCCACA CTGTGTTGGA AAATATATCC
    TTCTCTTTAA ACCCTGGACA AAGGGTAGGC TTATTAGGAA GAACTGGTTC AGGTAAAAGC
    ACCCTGTTGT CTGCCTTTTT GAGATTATTA TCAACCCAAG GAGACATTCA AATCGATGGG
    GTATCCTGGC AAACAATTCC ACTTCAGAAA TGGAGAAAGG CATTTGGAGT AGTTCCACAG
    AAAGTATTTA TATTTTCTGG AACTATTCGA AAAAACCTTG ATCCCTATGG AAAATGGAGT
    GATGAGGAAC TTTTGAAAGT CACAGAAGAG GTTGGTTTAA AGTCAATAAT TGATCAGTTT
    CCGGGACAGT TGGATTTTGT GCTTCTGGAT GGGGGTTGTG TCCTGAGCCA TGGCCACAAG
    CAACTCGTAT GTTTGGCTAG GTCGGTTCTC AGCAAAGCAA AAATCTTATT ACTCGATGAG
    CCTAGTGCAC ATTTGGATCC AATAACATTC CAAATAATTA GGAAAACACT AAAACATGCA
    TTTGCAAATT GCACAGTAAT TTTGTCAGAA CACAGACTAG AGGCAATGTT GGAATGCCAG
    CACTTTCTGG TGATTGAAGA CAATACAGTA CGACAGTATG ACTCTATTCA AAAGCTTATG
    AATGAGAAGA GTTTCTTCAA ACAAGCCATA AGCCAATCTG ATCGCTTGAA ACTATTCCCA
    TTGCATCGTA GAAATTCAAG TAAACGAAAA TCCAGACCCA AAATTTCTGC CTTACAAGAA
    GAGACAGAAG AGGAGGTTCA GGAAACAAGA CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.