gRNAs
The following cftr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cftr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OXa54776 | |
| Clone ID Related Accession (Same CDS sequence) | XM_031898971.1 | |
| Accession Version | XM_031898971.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-17 | |
| Organism | Xenopus tropicalis(tropical clawed frog) | |
| Product | cystic fibrosis transmembrane conductance regulator isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCAGAGAT CCCCTCTGGA GAAAGCCAGC ATCTTTTCAC AGATTTTCTT TAGCTGGACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_031754831.1 |
| CDS | 147..4604 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_031898971.1 |
1 | ATGCAGAGAT CCCCTCTGGA GAAAGCCAGC ATCTTTTCAC AGATTTTCTT TAGCTGGACC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OXa54777 | |
| Clone ID Related Accession (Same CDS sequence) | XM_012959952.3 | |
| Accession Version | XM_012959952.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-17 | |
| Organism | Xenopus tropicalis(tropical clawed frog) | |
| Product | cystic fibrosis transmembrane conductance regulator isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012959952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTTTTATA GCTGGACCAA GCCAATTTTG TGGAAAGGCT ATAGGCAACG GCTGGAACTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_012815406.2 |
| CDS | 1..4416 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_012959952.3 |
1 | ATGTTTTATA GCTGGACCAA GCCAATTTTG TGGAAAGGCT ATAGGCAACG GCTGGAACTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OXa33528 | |
| Clone ID Related Accession (Same CDS sequence) | XM_012959952.2 | |
| Accession Version | XM_012959952.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-09-07 | |
| Organism | Xenopus tropicalis(tropical clawed frog) | |
| Product | cystic fibrosis transmembrane conductance regulator | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012959952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTTTTATA GCTGGACCAA GCCAATTTTG TGGAAAGGCT ATAGGCAACG GCTGGAACTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_012815406.1 |
| CDS | 1..4416 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_012959952.2 |
1 | ATGTTTTATA GCTGGACCAA GCCAATTTTG TGGAAAGGCT ATAGGCAACG GCTGGAACTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
