nos3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nos3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nos3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa59575 XM_031903277.1
Latest version!
Xenopus tropicalis nitric oxide synthase 3 (nos3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa59575 XM_031903278.1
Latest version!
Xenopus tropicalis nitric oxide synthase 3 (nos3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa24972 NM_001256229.1
Latest version!
Xenopus tropicalis nitric oxide synthase 3 (nos3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa59575
    Clone ID Related Accession (Same CDS sequence) XM_031903278.1 , XM_031903277.1
    Accession Version XM_031903278.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nitric oxide synthase, endothelial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAACCTTC CTGACAAAAA GAGAGCAATA CGCTCCACCA ATGGGGGCCA AGCTCAGACC 
    CAGAAATGTA TTCGAGTCCG GAACTGGGAA AACAGAACCC TCTCTTACGA TACCCTGAAT
    GTGCAGACAG AGAAGGATGT TCCCTGCACC AAGTCCCGGT GCCTGGGTTC CGTTGTTTAC
    CCCTCCCAGC TGACCTGCCC ATTACCAGAA GGACCAAAGA GCCCGGAGGA GCTGCTACCC
    AAAGCCAAGG ATTTTATCAA CCAATATTAC AGCTCCATCA AGAGGGCCCA ATCTAAATCT
    CACCTGGAGA GACTGAAGGA GGTGGAAGAG GAGATTGTAT CCTCAGGAAC GTACCAGCTG
    AAGCAGAATG AACTGATATT TGGTGCCAAA CAGGCCTGGA GGAACGCCTC CCGCTGTGTG
    GGCCGGATAC AGTGGTCAAA GCTACAGGTG TTCGATGCTC GGGACTGCAG GACAGCACAT
    GAAATGTACA CCCACATCTG TAACCATATC AAATATGCCA CCAACAGGGG TAACATCAGA
    TCCGCCATCA CCATCTTCCC ACAAAGGACT GACGGAGCCT CAGATTTCAA GATCTGGAAC
    AGCCAGTTTA TTCGCTATGC GGGGTACAGG CAAGCGGACG ACACGGTGGT GGGGGACCCA
    GCCAACGCCG ATATCACTGA GCTTTGCATC CAGCTGGGTT GGAAACCCAA AGGTGGGAGG
    TTCGACGTGT TACCGTTACT GCTCCAGGCC AACGGGGATG ATCCAAAACT CTTCGAGATT
    CCACCTGAGC TGATATTGGA AGTACCACTC CGGCACCCCA CGTACGAATG GTTTGCAGAT
    TTGGGCCTGA AATGGTACGC CCTACCCGCC GTTAGCAACC TACTGCTGGA GATTGGCGGG
    CTGGAGTTTC CCGCCGTACC CTTCAACGGA TGGTACATGG GCACCGAAAT TGGCATGCGC
    AATCTGTGCG ACTCCTACAG ATACAATATA GCCGAGGAAG TAGCCAAGCG GCTGGGACTT
    GACACCAAGA AAACTTCCTC TCTATGGAAA GACAGAGCTG CCCTTGAAGT CAACATTGCT
    GTCTTGTACA GTTACCAGGC TGACAAAATC ACCATTGTGG ACCATCATGC AGCTACGGAG
    TCTTTCATGA AGCATATGGA ACATGAGATC CGTCAGAGAG GGGGATGTCC AGCTGACTGG
    GTTTGGATAG TACCACCCAC ATCCGCAAGC CTCACACCTG TGTTTCACCA GGAAATGGCC
    AATTACTATC TGTCTCCCTC TTTCCTCTAT CAGCCGGACC CATGGAAACA CCATGTCTGG
    AAGGGATCTC AGAGCACCAT CACCAGAAGA AGAACATTCA AGGAAGTGGC CAACGCAGTA
    AAATTTTCGG CCAAACTGAT GGGAAAAGCC ATGGCACGCC GAGTGAAAGC CACTATTCTC
    TATGCCACGG AAACCGGCAG GTCTGAGACC TACGCCCAGA AGCTCTGCAA GATATTCAGC
    TACGCGTTCG ACCCCAAGGT GATCTGCATG GACCAGTATG ATATTGTCAA CTTGGAGCAC
    GAGACTCTGG TTCTTGTGGT CACCAGCACA TTTGGTAATG GGGACCCTCC AGAAAATGGG
    GAGGCTTTTG CTCGGGAACT GATGGAGATG ACCTCCCCCA GCACATTGGC CCTGGCTCCA
    GAACAGCAGA AGAGCTACAA GATCCGGTTT AACAGTGTGT CCCAAGGGGA CCAGCTCGTC
    TCCTCCTGGA AGAATAAAAG GAAAGAATCT AGTAACACAG ACAGCGCGGG GCCCCTGGGC
    ACCCTCAGGT TCTCAGTTTT TGGCTTGGGG TCCCGGGCCT ACCCCCATTT CTGTGCATTT
    GGCCATGCTG TGGACACGAG GCTGGAAGAA CTCGGGGGAG AGAGGATCAT GGAGATGGGA
    GAAGGCGACG AGCTCTGTGG ACAGGAGGAC TCCTTTAGAA TTTGGGCCAA AGCTGTATTC
    AAGGCTGCCT GCGACACCTT CTGCGTTGGA GATGACCCAG ACCTTATAGT CAATGACTTG
    TTCCAAGCCA AAGGCAACTG GAAGAGGTCC CAGCACCGGC TGTCCATCCA GGTCTCAGCG
    CCCGACCTCA TCACAGGCCT CAGCCAGGTT CACAGAAGGA AAGTCTCGGA GGCCAAATTC
    ATTTCTAGGG AGAACTTGCA GAGCGAGAAG TCCAGCCGTT CCACCATCTT GGTTAAGCTC
    AGCATTGAGG AGCAGCCGGA ACTGAGTTAC TGCCCTGGGG ATCACCTGGG GATCTTCCCA
    TGTAACCGAG AGGAACTGGT GGTGGCTTTG TTGGAGAGAG TGGAAGATCC TCCTCCTTCC
    AATGACACGA TACAGGTGGA GACACTCAAC ACTGATCAAA ATAACAGGTT TGGCACCAGT
    CAGAAGATGA AATGGCTCTC AGACACTAGA ATACCTCCCT GTACCCTGAA GCAGGCACTT
    ACCAATTATC TGGATATTAC TACCCCTCCT ACACCTCAGC TGTTGCAGCT CCTCAGTGTG
    CTGTGCGAGG ATCTAGAGGA GAAACGGAGA ATGGAAACTC TCAGTCAGGA TTCCAGGCTG
    TACGAGGAGT GGAAGTGGTT TCGGTGTCCC ACTTTGGTGG AGGTTCTGGA GGAGTTTCCA
    TCGGTGAGTC TCCCCAGCTC CCTGCTACTG ACCCAACTGC CCCTCCTGCA GCCGCGCTAC
    TACTCCATCA GCTCCTCCTC GGACGTTTGC CCAGGGCAGA TCCACCTCAC TGTGGCTGTG
    GTGTCCTATC ATACGCAGGA CAATAAAGGG CCCCTGCACT ATGGCGTCTG CTCTACTTGG
    CTGGACCAGT TGGCTGCCGG GGACTCTGTG CCATGTTTTG TCCGTGGAGC TCCAACATTT
    CATTTGCCAT CTGACAAAAC TGCCCCATGC ATTCTGGTGG GTCCTGGCAC TGGAATTGCC
    CCATTCCGGG GCTTCTGGCA GCAGCAACTT TATGAAATTG AAAATAATGA TCAAGTGCCC
    GGCAACATGA CCCTGGTCTT CGGCTGCCGA TGTGCAGAAC TTGATCACAT CTACAAAGAG
    GAGACCCAAG AAGCCAAAAA CAAAGGAGCC CTGAGTGACA TCTACACGGC CTTCTCCCGG
    GAGCCTGGCA TGCCCAAGAC CTACGTGCAG GATATTTTGC GCACACAACT GGCGTGTGAG
    GTGTATGACG TTCTGTGTCA GAGCGGGGGC CACATGTATA TATGTGGCGA CGTCACAATG
    GCCACAGATG TTCTGCAAAC AGTTCAGCTG ATCCTTGTGT CTCAGGGGGA CATGAGCATC
    GTGGAGGCCG GAGAGTTTAT TGGGGAACTG AGGGACCAGA ACAGATACCA CGAGGACATC
    TTTGGCATCA CACTTAGGAC CCAGGAAGTG ACCACAAGGG TGCGGTGCCA GTCCATGTCC
    CTACAGGAGC GGAGAGAGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759138.1
    CDS154..3597
    Translation

    Target ORF information:

    RefSeq Version XM_031903278.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nitric oxide synthase 3 (nos3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903278.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAACCTTC CTGACAAAAA GAGAGCAATA CGCTCCACCA ATGGGGGCCA AGCTCAGACC 
    CAGAAATGTA TTCGAGTCCG GAACTGGGAA AACAGAACCC TCTCTTACGA TACCCTGAAT
    GTGCAGACAG AGAAGGATGT TCCCTGCACC AAGTCCCGGT GCCTGGGTTC CGTTGTTTAC
    CCCTCCCAGC TGACCTGCCC ATTACCAGAA GGACCAAAGA GCCCGGAGGA GCTGCTACCC
    AAAGCCAAGG ATTTTATCAA CCAATATTAC AGCTCCATCA AGAGGGCCCA ATCTAAATCT
    CACCTGGAGA GACTGAAGGA GGTGGAAGAG GAGATTGTAT CCTCAGGAAC GTACCAGCTG
    AAGCAGAATG AACTGATATT TGGTGCCAAA CAGGCCTGGA GGAACGCCTC CCGCTGTGTG
    GGCCGGATAC AGTGGTCAAA GCTACAGGTG TTCGATGCTC GGGACTGCAG GACAGCACAT
    GAAATGTACA CCCACATCTG TAACCATATC AAATATGCCA CCAACAGGGG TAACATCAGA
    TCCGCCATCA CCATCTTCCC ACAAAGGACT GACGGAGCCT CAGATTTCAA GATCTGGAAC
    AGCCAGTTTA TTCGCTATGC GGGGTACAGG CAAGCGGACG ACACGGTGGT GGGGGACCCA
    GCCAACGCCG ATATCACTGA GCTTTGCATC CAGCTGGGTT GGAAACCCAA AGGTGGGAGG
    TTCGACGTGT TACCGTTACT GCTCCAGGCC AACGGGGATG ATCCAAAACT CTTCGAGATT
    CCACCTGAGC TGATATTGGA AGTACCACTC CGGCACCCCA CGTACGAATG GTTTGCAGAT
    TTGGGCCTGA AATGGTACGC CCTACCCGCC GTTAGCAACC TACTGCTGGA GATTGGCGGG
    CTGGAGTTTC CCGCCGTACC CTTCAACGGA TGGTACATGG GCACCGAAAT TGGCATGCGC
    AATCTGTGCG ACTCCTACAG ATACAATATA GCCGAGGAAG TAGCCAAGCG GCTGGGACTT
    GACACCAAGA AAACTTCCTC TCTATGGAAA GACAGAGCTG CCCTTGAAGT CAACATTGCT
    GTCTTGTACA GTTACCAGGC TGACAAAATC ACCATTGTGG ACCATCATGC AGCTACGGAG
    TCTTTCATGA AGCATATGGA ACATGAGATC CGTCAGAGAG GGGGATGTCC AGCTGACTGG
    GTTTGGATAG TACCACCCAC ATCCGCAAGC CTCACACCTG TGTTTCACCA GGAAATGGCC
    AATTACTATC TGTCTCCCTC TTTCCTCTAT CAGCCGGACC CATGGAAACA CCATGTCTGG
    AAGGGATCTC AGAGCACCAT CACCAGAAGA AGAACATTCA AGGAAGTGGC CAACGCAGTA
    AAATTTTCGG CCAAACTGAT GGGAAAAGCC ATGGCACGCC GAGTGAAAGC CACTATTCTC
    TATGCCACGG AAACCGGCAG GTCTGAGACC TACGCCCAGA AGCTCTGCAA GATATTCAGC
    TACGCGTTCG ACCCCAAGGT GATCTGCATG GACCAGTATG ATATTGTCAA CTTGGAGCAC
    GAGACTCTGG TTCTTGTGGT CACCAGCACA TTTGGTAATG GGGACCCTCC AGAAAATGGG
    GAGGCTTTTG CTCGGGAACT GATGGAGATG ACCTCCCCCA GCACATTGGC CCTGGCTCCA
    GAACAGCAGA AGAGCTACAA GATCCGGTTT AACAGTGTGT CCCAAGGGGA CCAGCTCGTC
    TCCTCCTGGA AGAATAAAAG GAAAGAATCT AGTAACACAG ACAGCGCGGG GCCCCTGGGC
    ACCCTCAGGT TCTCAGTTTT TGGCTTGGGG TCCCGGGCCT ACCCCCATTT CTGTGCATTT
    GGCCATGCTG TGGACACGAG GCTGGAAGAA CTCGGGGGAG AGAGGATCAT GGAGATGGGA
    GAAGGCGACG AGCTCTGTGG ACAGGAGGAC TCCTTTAGAA TTTGGGCCAA AGCTGTATTC
    AAGGCTGCCT GCGACACCTT CTGCGTTGGA GATGACCCAG ACCTTATAGT CAATGACTTG
    TTCCAAGCCA AAGGCAACTG GAAGAGGTCC CAGCACCGGC TGTCCATCCA GGTCTCAGCG
    CCCGACCTCA TCACAGGCCT CAGCCAGGTT CACAGAAGGA AAGTCTCGGA GGCCAAATTC
    ATTTCTAGGG AGAACTTGCA GAGCGAGAAG TCCAGCCGTT CCACCATCTT GGTTAAGCTC
    AGCATTGAGG AGCAGCCGGA ACTGAGTTAC TGCCCTGGGG ATCACCTGGG GATCTTCCCA
    TGTAACCGAG AGGAACTGGT GGTGGCTTTG TTGGAGAGAG TGGAAGATCC TCCTCCTTCC
    AATGACACGA TACAGGTGGA GACACTCAAC ACTGATCAAA ATAACAGGTT TGGCACCAGT
    CAGAAGATGA AATGGCTCTC AGACACTAGA ATACCTCCCT GTACCCTGAA GCAGGCACTT
    ACCAATTATC TGGATATTAC TACCCCTCCT ACACCTCAGC TGTTGCAGCT CCTCAGTGTG
    CTGTGCGAGG ATCTAGAGGA GAAACGGAGA ATGGAAACTC TCAGTCAGGA TTCCAGGCTG
    TACGAGGAGT GGAAGTGGTT TCGGTGTCCC ACTTTGGTGG AGGTTCTGGA GGAGTTTCCA
    TCGGTGAGTC TCCCCAGCTC CCTGCTACTG ACCCAACTGC CCCTCCTGCA GCCGCGCTAC
    TACTCCATCA GCTCCTCCTC GGACGTTTGC CCAGGGCAGA TCCACCTCAC TGTGGCTGTG
    GTGTCCTATC ATACGCAGGA CAATAAAGGG CCCCTGCACT ATGGCGTCTG CTCTACTTGG
    CTGGACCAGT TGGCTGCCGG GGACTCTGTG CCATGTTTTG TCCGTGGAGC TCCAACATTT
    CATTTGCCAT CTGACAAAAC TGCCCCATGC ATTCTGGTGG GTCCTGGCAC TGGAATTGCC
    CCATTCCGGG GCTTCTGGCA GCAGCAACTT TATGAAATTG AAAATAATGA TCAAGTGCCC
    GGCAACATGA CCCTGGTCTT CGGCTGCCGA TGTGCAGAAC TTGATCACAT CTACAAAGAG
    GAGACCCAAG AAGCCAAAAA CAAAGGAGCC CTGAGTGACA TCTACACGGC CTTCTCCCGG
    GAGCCTGGCA TGCCCAAGAC CTACGTGCAG GATATTTTGC GCACACAACT GGCGTGTGAG
    GTGTATGACG TTCTGTGTCA GAGCGGGGGC CACATGTATA TATGTGGCGA CGTCACAATG
    GCCACAGATG TTCTGCAAAC AGTTCAGCTG ATCCTTGTGT CTCAGGGGGA CATGAGCATC
    GTGGAGGCCG GAGAGTTTAT TGGGGAACTG AGGGACCAGA ACAGATACCA CGAGGACATC
    TTTGGCATCA CACTTAGGAC CCAGGAAGTG ACCACAAGGG TGCGGTGCCA GTCCATGTCC
    CTACAGGAGC GGAGAGAGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59575
    Clone ID Related Accession (Same CDS sequence) XM_031903278.1 , XM_031903277.1
    Accession Version XM_031903277.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nitric oxide synthase, endothelial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAACCTTC CTGACAAAAA GAGAGCAATA CGCTCCACCA ATGGGGGCCA AGCTCAGACC 
    CAGAAATGTA TTCGAGTCCG GAACTGGGAA AACAGAACCC TCTCTTACGA TACCCTGAAT
    GTGCAGACAG AGAAGGATGT TCCCTGCACC AAGTCCCGGT GCCTGGGTTC CGTTGTTTAC
    CCCTCCCAGC TGACCTGCCC ATTACCAGAA GGACCAAAGA GCCCGGAGGA GCTGCTACCC
    AAAGCCAAGG ATTTTATCAA CCAATATTAC AGCTCCATCA AGAGGGCCCA ATCTAAATCT
    CACCTGGAGA GACTGAAGGA GGTGGAAGAG GAGATTGTAT CCTCAGGAAC GTACCAGCTG
    AAGCAGAATG AACTGATATT TGGTGCCAAA CAGGCCTGGA GGAACGCCTC CCGCTGTGTG
    GGCCGGATAC AGTGGTCAAA GCTACAGGTG TTCGATGCTC GGGACTGCAG GACAGCACAT
    GAAATGTACA CCCACATCTG TAACCATATC AAATATGCCA CCAACAGGGG TAACATCAGA
    TCCGCCATCA CCATCTTCCC ACAAAGGACT GACGGAGCCT CAGATTTCAA GATCTGGAAC
    AGCCAGTTTA TTCGCTATGC GGGGTACAGG CAAGCGGACG ACACGGTGGT GGGGGACCCA
    GCCAACGCCG ATATCACTGA GCTTTGCATC CAGCTGGGTT GGAAACCCAA AGGTGGGAGG
    TTCGACGTGT TACCGTTACT GCTCCAGGCC AACGGGGATG ATCCAAAACT CTTCGAGATT
    CCACCTGAGC TGATATTGGA AGTACCACTC CGGCACCCCA CGTACGAATG GTTTGCAGAT
    TTGGGCCTGA AATGGTACGC CCTACCCGCC GTTAGCAACC TACTGCTGGA GATTGGCGGG
    CTGGAGTTTC CCGCCGTACC CTTCAACGGA TGGTACATGG GCACCGAAAT TGGCATGCGC
    AATCTGTGCG ACTCCTACAG ATACAATATA GCCGAGGAAG TAGCCAAGCG GCTGGGACTT
    GACACCAAGA AAACTTCCTC TCTATGGAAA GACAGAGCTG CCCTTGAAGT CAACATTGCT
    GTCTTGTACA GTTACCAGGC TGACAAAATC ACCATTGTGG ACCATCATGC AGCTACGGAG
    TCTTTCATGA AGCATATGGA ACATGAGATC CGTCAGAGAG GGGGATGTCC AGCTGACTGG
    GTTTGGATAG TACCACCCAC ATCCGCAAGC CTCACACCTG TGTTTCACCA GGAAATGGCC
    AATTACTATC TGTCTCCCTC TTTCCTCTAT CAGCCGGACC CATGGAAACA CCATGTCTGG
    AAGGGATCTC AGAGCACCAT CACCAGAAGA AGAACATTCA AGGAAGTGGC CAACGCAGTA
    AAATTTTCGG CCAAACTGAT GGGAAAAGCC ATGGCACGCC GAGTGAAAGC CACTATTCTC
    TATGCCACGG AAACCGGCAG GTCTGAGACC TACGCCCAGA AGCTCTGCAA GATATTCAGC
    TACGCGTTCG ACCCCAAGGT GATCTGCATG GACCAGTATG ATATTGTCAA CTTGGAGCAC
    GAGACTCTGG TTCTTGTGGT CACCAGCACA TTTGGTAATG GGGACCCTCC AGAAAATGGG
    GAGGCTTTTG CTCGGGAACT GATGGAGATG ACCTCCCCCA GCACATTGGC CCTGGCTCCA
    GAACAGCAGA AGAGCTACAA GATCCGGTTT AACAGTGTGT CCCAAGGGGA CCAGCTCGTC
    TCCTCCTGGA AGAATAAAAG GAAAGAATCT AGTAACACAG ACAGCGCGGG GCCCCTGGGC
    ACCCTCAGGT TCTCAGTTTT TGGCTTGGGG TCCCGGGCCT ACCCCCATTT CTGTGCATTT
    GGCCATGCTG TGGACACGAG GCTGGAAGAA CTCGGGGGAG AGAGGATCAT GGAGATGGGA
    GAAGGCGACG AGCTCTGTGG ACAGGAGGAC TCCTTTAGAA TTTGGGCCAA AGCTGTATTC
    AAGGCTGCCT GCGACACCTT CTGCGTTGGA GATGACCCAG ACCTTATAGT CAATGACTTG
    TTCCAAGCCA AAGGCAACTG GAAGAGGTCC CAGCACCGGC TGTCCATCCA GGTCTCAGCG
    CCCGACCTCA TCACAGGCCT CAGCCAGGTT CACAGAAGGA AAGTCTCGGA GGCCAAATTC
    ATTTCTAGGG AGAACTTGCA GAGCGAGAAG TCCAGCCGTT CCACCATCTT GGTTAAGCTC
    AGCATTGAGG AGCAGCCGGA ACTGAGTTAC TGCCCTGGGG ATCACCTGGG GATCTTCCCA
    TGTAACCGAG AGGAACTGGT GGTGGCTTTG TTGGAGAGAG TGGAAGATCC TCCTCCTTCC
    AATGACACGA TACAGGTGGA GACACTCAAC ACTGATCAAA ATAACAGGTT TGGCACCAGT
    CAGAAGATGA AATGGCTCTC AGACACTAGA ATACCTCCCT GTACCCTGAA GCAGGCACTT
    ACCAATTATC TGGATATTAC TACCCCTCCT ACACCTCAGC TGTTGCAGCT CCTCAGTGTG
    CTGTGCGAGG ATCTAGAGGA GAAACGGAGA ATGGAAACTC TCAGTCAGGA TTCCAGGCTG
    TACGAGGAGT GGAAGTGGTT TCGGTGTCCC ACTTTGGTGG AGGTTCTGGA GGAGTTTCCA
    TCGGTGAGTC TCCCCAGCTC CCTGCTACTG ACCCAACTGC CCCTCCTGCA GCCGCGCTAC
    TACTCCATCA GCTCCTCCTC GGACGTTTGC CCAGGGCAGA TCCACCTCAC TGTGGCTGTG
    GTGTCCTATC ATACGCAGGA CAATAAAGGG CCCCTGCACT ATGGCGTCTG CTCTACTTGG
    CTGGACCAGT TGGCTGCCGG GGACTCTGTG CCATGTTTTG TCCGTGGAGC TCCAACATTT
    CATTTGCCAT CTGACAAAAC TGCCCCATGC ATTCTGGTGG GTCCTGGCAC TGGAATTGCC
    CCATTCCGGG GCTTCTGGCA GCAGCAACTT TATGAAATTG AAAATAATGA TCAAGTGCCC
    GGCAACATGA CCCTGGTCTT CGGCTGCCGA TGTGCAGAAC TTGATCACAT CTACAAAGAG
    GAGACCCAAG AAGCCAAAAA CAAAGGAGCC CTGAGTGACA TCTACACGGC CTTCTCCCGG
    GAGCCTGGCA TGCCCAAGAC CTACGTGCAG GATATTTTGC GCACACAACT GGCGTGTGAG
    GTGTATGACG TTCTGTGTCA GAGCGGGGGC CACATGTATA TATGTGGCGA CGTCACAATG
    GCCACAGATG TTCTGCAAAC AGTTCAGCTG ATCCTTGTGT CTCAGGGGGA CATGAGCATC
    GTGGAGGCCG GAGAGTTTAT TGGGGAACTG AGGGACCAGA ACAGATACCA CGAGGACATC
    TTTGGCATCA CACTTAGGAC CCAGGAAGTG ACCACAAGGG TGCGGTGCCA GTCCATGTCC
    CTACAGGAGC GGAGAGAGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759137.1
    CDS116..3559
    Translation

    Target ORF information:

    RefSeq Version XM_031903277.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nitric oxide synthase 3 (nos3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903277.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAACCTTC CTGACAAAAA GAGAGCAATA CGCTCCACCA ATGGGGGCCA AGCTCAGACC 
    CAGAAATGTA TTCGAGTCCG GAACTGGGAA AACAGAACCC TCTCTTACGA TACCCTGAAT
    GTGCAGACAG AGAAGGATGT TCCCTGCACC AAGTCCCGGT GCCTGGGTTC CGTTGTTTAC
    CCCTCCCAGC TGACCTGCCC ATTACCAGAA GGACCAAAGA GCCCGGAGGA GCTGCTACCC
    AAAGCCAAGG ATTTTATCAA CCAATATTAC AGCTCCATCA AGAGGGCCCA ATCTAAATCT
    CACCTGGAGA GACTGAAGGA GGTGGAAGAG GAGATTGTAT CCTCAGGAAC GTACCAGCTG
    AAGCAGAATG AACTGATATT TGGTGCCAAA CAGGCCTGGA GGAACGCCTC CCGCTGTGTG
    GGCCGGATAC AGTGGTCAAA GCTACAGGTG TTCGATGCTC GGGACTGCAG GACAGCACAT
    GAAATGTACA CCCACATCTG TAACCATATC AAATATGCCA CCAACAGGGG TAACATCAGA
    TCCGCCATCA CCATCTTCCC ACAAAGGACT GACGGAGCCT CAGATTTCAA GATCTGGAAC
    AGCCAGTTTA TTCGCTATGC GGGGTACAGG CAAGCGGACG ACACGGTGGT GGGGGACCCA
    GCCAACGCCG ATATCACTGA GCTTTGCATC CAGCTGGGTT GGAAACCCAA AGGTGGGAGG
    TTCGACGTGT TACCGTTACT GCTCCAGGCC AACGGGGATG ATCCAAAACT CTTCGAGATT
    CCACCTGAGC TGATATTGGA AGTACCACTC CGGCACCCCA CGTACGAATG GTTTGCAGAT
    TTGGGCCTGA AATGGTACGC CCTACCCGCC GTTAGCAACC TACTGCTGGA GATTGGCGGG
    CTGGAGTTTC CCGCCGTACC CTTCAACGGA TGGTACATGG GCACCGAAAT TGGCATGCGC
    AATCTGTGCG ACTCCTACAG ATACAATATA GCCGAGGAAG TAGCCAAGCG GCTGGGACTT
    GACACCAAGA AAACTTCCTC TCTATGGAAA GACAGAGCTG CCCTTGAAGT CAACATTGCT
    GTCTTGTACA GTTACCAGGC TGACAAAATC ACCATTGTGG ACCATCATGC AGCTACGGAG
    TCTTTCATGA AGCATATGGA ACATGAGATC CGTCAGAGAG GGGGATGTCC AGCTGACTGG
    GTTTGGATAG TACCACCCAC ATCCGCAAGC CTCACACCTG TGTTTCACCA GGAAATGGCC
    AATTACTATC TGTCTCCCTC TTTCCTCTAT CAGCCGGACC CATGGAAACA CCATGTCTGG
    AAGGGATCTC AGAGCACCAT CACCAGAAGA AGAACATTCA AGGAAGTGGC CAACGCAGTA
    AAATTTTCGG CCAAACTGAT GGGAAAAGCC ATGGCACGCC GAGTGAAAGC CACTATTCTC
    TATGCCACGG AAACCGGCAG GTCTGAGACC TACGCCCAGA AGCTCTGCAA GATATTCAGC
    TACGCGTTCG ACCCCAAGGT GATCTGCATG GACCAGTATG ATATTGTCAA CTTGGAGCAC
    GAGACTCTGG TTCTTGTGGT CACCAGCACA TTTGGTAATG GGGACCCTCC AGAAAATGGG
    GAGGCTTTTG CTCGGGAACT GATGGAGATG ACCTCCCCCA GCACATTGGC CCTGGCTCCA
    GAACAGCAGA AGAGCTACAA GATCCGGTTT AACAGTGTGT CCCAAGGGGA CCAGCTCGTC
    TCCTCCTGGA AGAATAAAAG GAAAGAATCT AGTAACACAG ACAGCGCGGG GCCCCTGGGC
    ACCCTCAGGT TCTCAGTTTT TGGCTTGGGG TCCCGGGCCT ACCCCCATTT CTGTGCATTT
    GGCCATGCTG TGGACACGAG GCTGGAAGAA CTCGGGGGAG AGAGGATCAT GGAGATGGGA
    GAAGGCGACG AGCTCTGTGG ACAGGAGGAC TCCTTTAGAA TTTGGGCCAA AGCTGTATTC
    AAGGCTGCCT GCGACACCTT CTGCGTTGGA GATGACCCAG ACCTTATAGT CAATGACTTG
    TTCCAAGCCA AAGGCAACTG GAAGAGGTCC CAGCACCGGC TGTCCATCCA GGTCTCAGCG
    CCCGACCTCA TCACAGGCCT CAGCCAGGTT CACAGAAGGA AAGTCTCGGA GGCCAAATTC
    ATTTCTAGGG AGAACTTGCA GAGCGAGAAG TCCAGCCGTT CCACCATCTT GGTTAAGCTC
    AGCATTGAGG AGCAGCCGGA ACTGAGTTAC TGCCCTGGGG ATCACCTGGG GATCTTCCCA
    TGTAACCGAG AGGAACTGGT GGTGGCTTTG TTGGAGAGAG TGGAAGATCC TCCTCCTTCC
    AATGACACGA TACAGGTGGA GACACTCAAC ACTGATCAAA ATAACAGGTT TGGCACCAGT
    CAGAAGATGA AATGGCTCTC AGACACTAGA ATACCTCCCT GTACCCTGAA GCAGGCACTT
    ACCAATTATC TGGATATTAC TACCCCTCCT ACACCTCAGC TGTTGCAGCT CCTCAGTGTG
    CTGTGCGAGG ATCTAGAGGA GAAACGGAGA ATGGAAACTC TCAGTCAGGA TTCCAGGCTG
    TACGAGGAGT GGAAGTGGTT TCGGTGTCCC ACTTTGGTGG AGGTTCTGGA GGAGTTTCCA
    TCGGTGAGTC TCCCCAGCTC CCTGCTACTG ACCCAACTGC CCCTCCTGCA GCCGCGCTAC
    TACTCCATCA GCTCCTCCTC GGACGTTTGC CCAGGGCAGA TCCACCTCAC TGTGGCTGTG
    GTGTCCTATC ATACGCAGGA CAATAAAGGG CCCCTGCACT ATGGCGTCTG CTCTACTTGG
    CTGGACCAGT TGGCTGCCGG GGACTCTGTG CCATGTTTTG TCCGTGGAGC TCCAACATTT
    CATTTGCCAT CTGACAAAAC TGCCCCATGC ATTCTGGTGG GTCCTGGCAC TGGAATTGCC
    CCATTCCGGG GCTTCTGGCA GCAGCAACTT TATGAAATTG AAAATAATGA TCAAGTGCCC
    GGCAACATGA CCCTGGTCTT CGGCTGCCGA TGTGCAGAAC TTGATCACAT CTACAAAGAG
    GAGACCCAAG AAGCCAAAAA CAAAGGAGCC CTGAGTGACA TCTACACGGC CTTCTCCCGG
    GAGCCTGGCA TGCCCAAGAC CTACGTGCAG GATATTTTGC GCACACAACT GGCGTGTGAG
    GTGTATGACG TTCTGTGTCA GAGCGGGGGC CACATGTATA TATGTGGCGA CGTCACAATG
    GCCACAGATG TTCTGCAAAC AGTTCAGCTG ATCCTTGTGT CTCAGGGGGA CATGAGCATC
    GTGGAGGCCG GAGAGTTTAT TGGGGAACTG AGGGACCAGA ACAGATACCA CGAGGACATC
    TTTGGCATCA CACTTAGGAC CCAGGAAGTG ACCACAAGGG TGCGGTGCCA GTCCATGTCC
    CTACAGGAGC GGAGAGAGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa24972
    Clone ID Related Accession (Same CDS sequence) NM_001256229.1
    Accession Version NM_001256229.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3534bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nitric oxide synthase, endothelial
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ665981.1. On Jan 14, 2012 this sequence version replaced XM_002943012.1. ##Evidence-Data-START## Transcript exon combination :: FJ665981.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028291, SAMD00028292 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGGAGCT CGGCCAGTAG CCTCAAGGGC CTGCGCGCCC CCCTCTCCCT AAACTTCTGC 
    CGCAAAGAGG GGACCCTGCC CGACGAGGAG GAGGGGGCAC TGACGGACAG GGAGAGTGAG
    GGCTCCACCA ATGGGGGCCA AGCTCAGACC CAGAAATGTA TTCGAGTCCG GAACTGGGAA
    AACAGAACCC TCTCTTACGA TACCCTGAAT GTGCAGACAG AGAAGGATGT TCCCTGCACC
    AAGTCCCGGT GCCTGGGTTC CGTTGTTTAC CCCTCCCAGC TGACCTGCCC ATTACCAGAA
    GGACCAAAGA GCCCGGAGGA GCTGCTACCC AAAGCCAAGG ATTTTATCAA CCAATATTAC
    AGCTCCATCA AGAGGGCCCA ATCTAAATCT CACCTGGAGA GACTGAAGGA GGTGGAAGAG
    GAGATTGTAT CCTCAGGAAC GTACCAGCTG AAGCAGAATG AACTGATATT TGGTGCCAAA
    CAGGCCTGGA GGAACGCCTC CCGCTGTGTG GGCCGGATAC AGTGGTCAAA GCTACAGGTG
    TTCGATGCTC GGGACTGCAG GACAGCACAT GAAATGTACA CCCACATCTG TAACCATATC
    AAATATGCCA CCAACAGGGG TAACATCAGA TCCGCCATCA CCATCTTCCC ACAAAGGACT
    GACGGAGCCT CAGATTTCAA GATCTGGAAC AGCCAGTTTA TTCGCTATGC GGGGTACAGG
    CAAGCGGACG ACACGGTGGT GGGGGACCCA GCCAACGCCG ATATCACTGA GCTTTGCATC
    CAGCTGGGTT GGAAACCCAA AGGTGGGAGG TTCGACGTGT TACCGTTACT GCTCCAGGCC
    AACGGGGATG ATCCAAAACT CTTCGAGATT CCACCTGAGC TGATATTGGA AGTACCACTC
    CGGCACCCCA CGTACGAATG GTTTGCAGAT TTGGGCCTGA AATGGTACGC CCTACCCGCC
    GTTAGCAACC TACTGCTGGA GATTGGCGGG CTGGAGTTTC CCGCCGTACC CTTCAACGGA
    TGGTACATGG GCACCGAAAT TGGCATGCGC AATCTGTGCG ACTCCTACAG ATACAATATA
    GCCGAGGAAG TAGCCAAGCG GCTGGGACTT GACACCAAGA AAACTTCCTC TCTATGGAAA
    GACAGAGCTG CCCTTGAAGT CAACATTGCT GTCTTGTACA GTTACCAGGC TGACAAAATC
    ACCATTGTGG ACCATCATGC AGCTACGGAG TCTTTCATGA AGCATATGGA ACATGAGATC
    CGTCAGAGAG GGGGATGTCC AGCTGACTGG GTTTGGATAG TACCACCCAC ATCCGCAAGC
    CTCACACCTG TGTTTCACCA GGAAATGGCC AATTACTATC TGTCTCCCTC TTTCCTCTAT
    CAGCCGGACC CATGGAAACA CCATGTCTGG AAGGGATCTC AGAGCACCAT CACCAGAAGA
    AGAACATTCA AGGAAGTGGC CAACGCAGTA AAATTTTCGG CCAAACTGAT GGGAAAAGCC
    ATGGCACGCC GAGTGAAAGC CACTATTCTC TATGCCACGG AAACCGGCAG GTCTGAGACC
    TACGCCCAGA AGCTCTGCAA GATATTCAGC TACGCGTTCG ACCCCAAGGT GATCTGCATG
    GACCAGTATG ATATTGTCAA CTTGGAGCAC GAGACTCTGG TTCTTGTGGT CACCAGCACA
    TTTGGTAATG GGGACCCTCC AGAAAATGGG GAGGCTTTTG CTCGGGAACT GATGGAGATG
    ACCTCCCCCA GCACATTGGC CCTGGCTCCA GAACAGCAGA AGAGCTACAA GATCCGGTTT
    AACAGTGTGT CCCAAGGGGA CCAGCTCGTC TCCTCCTGGA AGAATAAAAG GAAAGAATCT
    AGTAACACAG ACAGCGCGGG GCCCCTGGGC ACCCTCAGGT TCTCAGTTTT TGGCTTGGGG
    TCCCGGGCCT ACCCCCATTT CTGTGCATTT GGCCATGCTG TGGACACGAG GCTGGAAGAA
    CTCGGGGGAG AGAGGATCAT GGAGATGGGA GAAGGCGACG AGCTCTGTGG ACAGGAGGAC
    TCCTTTAGAA TTTGGGCCAA AGCTGTATTC AAGGCTGCCT GCGACACCTT CTGCGTTGGA
    GATGACCCAG ACCTTATAGT CAATGACTTG TTCCAAGCCA AAGGCAACTG GAAGAGGTCC
    CAGCACCGGC TGTCCATCCA GGTCTCAGCG CCCGACCTCA TCACAGGCCT CAGCCAGGTT
    CACAGAAGGA AAGTCTCGGA GGCCAAATTC ATTTCTAGGG AGAACTTGCA GAGCGAGAAG
    TCCAGCCGTT CCACCATCTT GGTTAAGCTC AGCATTGAGG AGCAGCCGGA ACTGAGTTAC
    TGCCCTGGGG ATCACCTGGG GATCTTCCCA TGTAACCGAG AGGAACTGGT GGTGGCTTTG
    TTGGAGAGAG TGGAAGATCC TCCTCCTTCC AATGACACGA TACAGGTGGA GACACTCAAC
    ACTGATCAAA ATAACAGGTT TGGCACCAGT CAGAAGATGA AATGGCTCTC AGACACTAGA
    ATACCTCCCT GTACCCTGAA GCAGGCACTT ACCAATTATC TGGATATTAC TACCCCTCCT
    ACACCTCAGC TGTTGCAGCT CCTCAGTGTG CTGTGCGAGG ATCTAGAGGA GAAACGGAGA
    ATGGAAACTC TCAGTCAGGA TTCCAGGCTG TACGAGGAGT GGAAGTGGTT TCGGTGTCCC
    ACTTTGGTGG AGGTTCTGGA GGAGTTTCCA TCGGTGAGTC TCCCCAGCTC CCTGCTACTG
    ACCCAACTGC CCCTCCTGCA GCCGCGCTAC TACTCCATCA GCTCCTCCTC GGACGTTTGC
    CCAGGGCAGA TCCACCTCAC TGTGGCTGTG GTGTCCTATC ATACGCAGGA CAATAAAGGG
    CCCCTGCACT ATGGCGTCTG CTCTACTTGG CTGGACCAGT TGGCTGCCGG GGACTCTGTG
    CCATGTTTTG TCCGTGGAGC TCCAACATTT CATTTGCCAT CTGACAAAAC TGCCCCATGC
    ATTCTGGTGG GTCCTGGCAC TGGAATTGCC CCATTCCGGG GCTTCTGGCA GCAGCAACTT
    TATGAAATTG AAAATAATGA TCAAGTGCCC GGCAACATGA CCCTGGTCTT CGGCTGCCGA
    TGTGCAGAAC TTGATCACAT CTACAAAGAG GAGACCCAAG AAGCCAAAAA CAAAGGAGCC
    CTGAGTGACA TCTACACGGC CTTCTCCCGG GAGCCTGGCA TGCCCAAGAC CTACGTGCAG
    GATATTTTGC GCACACAACT GGCGTGTGAG GTGTATGACG TTCTGTGTCA GAGCGGGGGC
    CACATGTATA TATGTGGCGA CGTCACAATG GCCACAGATG TTCTGCAAAC AGTTCAGCTG
    ATCCTTGTGT CTCAGGGGGA CATGAGCATC GTGGAGGCCG GAGAGTTTAT TGGGGAACTG
    AGGGACCAGA ACAGATACCA CGAGGACATC TTTGGCATCA CACTTAGGAC CCAGGAAGTG
    ACCACAAGGG TGCGGTGCCA GTCCATGTCC CTACAGGAGC GGAGAGAGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001243158.1
    CDS287..3820
    Translation

    Target ORF information:

    RefSeq Version NM_001256229.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nitric oxide synthase 3 (nos3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001256229.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGGAGCT CGGCCAGTAG CCTCAAGGGC CTGCGCGCCC CCCTCTCCCT AAACTTCTGC 
    CGCAAAGAGG GGACCCTGCC CGACGAGGAG GAGGGGGCAC TGACGGACAG GGAGAGTGAG
    GGCTCCACCA ATGGGGGCCA AGCTCAGACC CAGAAATGTA TTCGAGTCCG GAACTGGGAA
    AACAGAACCC TCTCTTACGA TACCCTGAAT GTGCAGACAG AGAAGGATGT TCCCTGCACC
    AAGTCCCGGT GCCTGGGTTC CGTTGTTTAC CCCTCCCAGC TGACCTGCCC ATTACCAGAA
    GGACCAAAGA GCCCGGAGGA GCTGCTACCC AAAGCCAAGG ATTTTATCAA CCAATATTAC
    AGCTCCATCA AGAGGGCCCA ATCTAAATCT CACCTGGAGA GACTGAAGGA GGTGGAAGAG
    GAGATTGTAT CCTCAGGAAC GTACCAGCTG AAGCAGAATG AACTGATATT TGGTGCCAAA
    CAGGCCTGGA GGAACGCCTC CCGCTGTGTG GGCCGGATAC AGTGGTCAAA GCTACAGGTG
    TTCGATGCTC GGGACTGCAG GACAGCACAT GAAATGTACA CCCACATCTG TAACCATATC
    AAATATGCCA CCAACAGGGG TAACATCAGA TCCGCCATCA CCATCTTCCC ACAAAGGACT
    GACGGAGCCT CAGATTTCAA GATCTGGAAC AGCCAGTTTA TTCGCTATGC GGGGTACAGG
    CAAGCGGACG ACACGGTGGT GGGGGACCCA GCCAACGCCG ATATCACTGA GCTTTGCATC
    CAGCTGGGTT GGAAACCCAA AGGTGGGAGG TTCGACGTGT TACCGTTACT GCTCCAGGCC
    AACGGGGATG ATCCAAAACT CTTCGAGATT CCACCTGAGC TGATATTGGA AGTACCACTC
    CGGCACCCCA CGTACGAATG GTTTGCAGAT TTGGGCCTGA AATGGTACGC CCTACCCGCC
    GTTAGCAACC TACTGCTGGA GATTGGCGGG CTGGAGTTTC CCGCCGTACC CTTCAACGGA
    TGGTACATGG GCACCGAAAT TGGCATGCGC AATCTGTGCG ACTCCTACAG ATACAATATA
    GCCGAGGAAG TAGCCAAGCG GCTGGGACTT GACACCAAGA AAACTTCCTC TCTATGGAAA
    GACAGAGCTG CCCTTGAAGT CAACATTGCT GTCTTGTACA GTTACCAGGC TGACAAAATC
    ACCATTGTGG ACCATCATGC AGCTACGGAG TCTTTCATGA AGCATATGGA ACATGAGATC
    CGTCAGAGAG GGGGATGTCC AGCTGACTGG GTTTGGATAG TACCACCCAC ATCCGCAAGC
    CTCACACCTG TGTTTCACCA GGAAATGGCC AATTACTATC TGTCTCCCTC TTTCCTCTAT
    CAGCCGGACC CATGGAAACA CCATGTCTGG AAGGGATCTC AGAGCACCAT CACCAGAAGA
    AGAACATTCA AGGAAGTGGC CAACGCAGTA AAATTTTCGG CCAAACTGAT GGGAAAAGCC
    ATGGCACGCC GAGTGAAAGC CACTATTCTC TATGCCACGG AAACCGGCAG GTCTGAGACC
    TACGCCCAGA AGCTCTGCAA GATATTCAGC TACGCGTTCG ACCCCAAGGT GATCTGCATG
    GACCAGTATG ATATTGTCAA CTTGGAGCAC GAGACTCTGG TTCTTGTGGT CACCAGCACA
    TTTGGTAATG GGGACCCTCC AGAAAATGGG GAGGCTTTTG CTCGGGAACT GATGGAGATG
    ACCTCCCCCA GCACATTGGC CCTGGCTCCA GAACAGCAGA AGAGCTACAA GATCCGGTTT
    AACAGTGTGT CCCAAGGGGA CCAGCTCGTC TCCTCCTGGA AGAATAAAAG GAAAGAATCT
    AGTAACACAG ACAGCGCGGG GCCCCTGGGC ACCCTCAGGT TCTCAGTTTT TGGCTTGGGG
    TCCCGGGCCT ACCCCCATTT CTGTGCATTT GGCCATGCTG TGGACACGAG GCTGGAAGAA
    CTCGGGGGAG AGAGGATCAT GGAGATGGGA GAAGGCGACG AGCTCTGTGG ACAGGAGGAC
    TCCTTTAGAA TTTGGGCCAA AGCTGTATTC AAGGCTGCCT GCGACACCTT CTGCGTTGGA
    GATGACCCAG ACCTTATAGT CAATGACTTG TTCCAAGCCA AAGGCAACTG GAAGAGGTCC
    CAGCACCGGC TGTCCATCCA GGTCTCAGCG CCCGACCTCA TCACAGGCCT CAGCCAGGTT
    CACAGAAGGA AAGTCTCGGA GGCCAAATTC ATTTCTAGGG AGAACTTGCA GAGCGAGAAG
    TCCAGCCGTT CCACCATCTT GGTTAAGCTC AGCATTGAGG AGCAGCCGGA ACTGAGTTAC
    TGCCCTGGGG ATCACCTGGG GATCTTCCCA TGTAACCGAG AGGAACTGGT GGTGGCTTTG
    TTGGAGAGAG TGGAAGATCC TCCTCCTTCC AATGACACGA TACAGGTGGA GACACTCAAC
    ACTGATCAAA ATAACAGGTT TGGCACCAGT CAGAAGATGA AATGGCTCTC AGACACTAGA
    ATACCTCCCT GTACCCTGAA GCAGGCACTT ACCAATTATC TGGATATTAC TACCCCTCCT
    ACACCTCAGC TGTTGCAGCT CCTCAGTGTG CTGTGCGAGG ATCTAGAGGA GAAACGGAGA
    ATGGAAACTC TCAGTCAGGA TTCCAGGCTG TACGAGGAGT GGAAGTGGTT TCGGTGTCCC
    ACTTTGGTGG AGGTTCTGGA GGAGTTTCCA TCGGTGAGTC TCCCCAGCTC CCTGCTACTG
    ACCCAACTGC CCCTCCTGCA GCCGCGCTAC TACTCCATCA GCTCCTCCTC GGACGTTTGC
    CCAGGGCAGA TCCACCTCAC TGTGGCTGTG GTGTCCTATC ATACGCAGGA CAATAAAGGG
    CCCCTGCACT ATGGCGTCTG CTCTACTTGG CTGGACCAGT TGGCTGCCGG GGACTCTGTG
    CCATGTTTTG TCCGTGGAGC TCCAACATTT CATTTGCCAT CTGACAAAAC TGCCCCATGC
    ATTCTGGTGG GTCCTGGCAC TGGAATTGCC CCATTCCGGG GCTTCTGGCA GCAGCAACTT
    TATGAAATTG AAAATAATGA TCAAGTGCCC GGCAACATGA CCCTGGTCTT CGGCTGCCGA
    TGTGCAGAAC TTGATCACAT CTACAAAGAG GAGACCCAAG AAGCCAAAAA CAAAGGAGCC
    CTGAGTGACA TCTACACGGC CTTCTCCCGG GAGCCTGGCA TGCCCAAGAC CTACGTGCAG
    GATATTTTGC GCACACAACT GGCGTGTGAG GTGTATGACG TTCTGTGTCA GAGCGGGGGC
    CACATGTATA TATGTGGCGA CGTCACAATG GCCACAGATG TTCTGCAAAC AGTTCAGCTG
    ATCCTTGTGT CTCAGGGGGA CATGAGCATC GTGGAGGCCG GAGAGTTTAT TGGGGAACTG
    AGGGACCAGA ACAGATACCA CGAGGACATC TTTGGCATCA CACTTAGGAC CCAGGAAGTG
    ACCACAAGGG TGCGGTGCCA GTCCATGTCC CTACAGGAGC GGAGAGAGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.