polr1b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	polr1b
Entrez Gene ID	100485503
Full Name	polymerase (RNA) I polypeptide B
General protein information	Preferred Names polymerase (RNA) I polypeptide B Names DNA-directed RNA polymerase I subunit RPA2 polymerase (RNA) I polypeptide B, 128kDa
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002940396.5	XP_002940442.1	DNA-directed RNA polymerase I subunit RPA2
XM_002940396.4	XP_002940442.1	DNA-directed RNA polymerase I subunit RPA2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	POLR1B	NP_001093762.1
Rattus norvegicus (Norway rat)	Polr1b	NP_113961.1
Drosophila melanogaster (fruit fly)	RpI135	NP_476708.1
Eremothecium gossypii	AGOS_ABR029W	NP_982975.2
Pan troglodytes (chimpanzee)	POLR1B	XP_001145399.1
Canis lupus familiaris (dog)	POLR1B	XP_003639648.1
Saccharomyces cerevisiae (baker's yeast)	RPA135	NP_015335.1
Neurospora crassa	NCU08616	XP_964824.1
Mus musculus (house mouse)	Polr1b	NP_033112.2
Kluyveromyces lactis	KLLA0B06303g	XP_451816.1
Homo sapiens (human)	POLR1B	NP_001269701.1
Gallus gallus (chicken)	POLR1B	XP_004935307.1
Caenorhabditis elegans (roundworm)	F14B4.3	NP_492476.1
Xenopus tropicalis (tropical clawed frog)	polr1b	XP_002940442.1
	POLR1B	XP_001088474.1
Danio rerio (zebrafish)	polr1b	NP_956812.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009539	XP_310152.4
Schizosaccharomyces pombe (fission yeast)	rpa2	NP_595819.2
Magnaporthe oryzae (rice blast fungus)	MGG_01572	XP_003714550.1
Arabidopsis thaliana (thale cress)	NRPA2	NP_564341.2

Gene Ontology

The following polr1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the polr1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10754	XM_002940396.5 Latest version!	Xenopus tropicalis polymerase (RNA) I polypeptide B (polr1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa10754	XM_002940396.4	Xenopus tropicalis polymerase (RNA) I polypeptide B (polr1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

polr1b ( XM_002940396.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940442.1 Xenopus tropicalis polymerase (RNA) I polypeptide B (polr1b), mRNA.

Price & Availability↑

CloneID	OXa10754
Clone ID Related Accession (Same CDS sequence)	XM_002940396.5 , XM_002940396.4
Accession Version	XM_002940396.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA-directed RNA polymerase I subunit RPA2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940396.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGG ACACTAAGTG GCAAGCGCTG CTCAGGGAGC CTAGCCTGGG CAAACTTACC 
AGTCCCGACT ATGGGATTCC CGGGGAGAGC CAGCACCCTC CTCTGCAGGA TATCACTCGG 
GCTCATATCG ACTCCTTCAA CCAAGCAATG ACAGAGGGGC TGAGTCTGGC GGTGCAGGCT 
ATGAAACCAC TTGAGTTTAC TTACAAAAAT GATAAGGTGG CATTGCAAAT AGTGGATGCA 
GTTATTACCC CTCCTCTAGT GCCCAAAGGA AGCATCTGCA AAGAGCAAAA GATATACCCA 
GCAGAATGCA GAGGAAGAAG AAGTACATAC CGGGGCAAGC TGACTGCCGA TGTAAGCTGG 
TCCTTAAATG GATTGCAAAA AGGCATCATC AAACAAACCC TGGGACATAT TCCTATCATG 
GTGAAGTCAA AATTTTGCAA CCTGTATGAC CTTAACCCAA AACAACTTCT TGAGCACCAC 
GAGGAAGAGC AAGAAATGGG AGGATATTTC ATTATAAATG GAATAGAAAA GGTTATCAGA 
ATGCTTATTA TGCCTCGAAG GAACTTTCCC ATCGCCATGA TTCGTCCCAA GTGGAAGAAT 
AGAGGGCCTG GTTACACTCA GTATGGTATT TCACTTCGTT GTGTCAGAGA TGAACATACT 
GCTGTCAACA TGAACCTGCA CTATCTAGAG AATGGCTCTG TTATGGTGAA TTTCATCTAT 
CAAAAGGAGC TCTTCTTCAT TCCACTGGGC TTTGCATTAA AGGCTCTAGT TGACTATTCA 
GATTATCAGA TTTTCACTGA ATTGATCAAA GGCAAGGAAG AGAATTCATT CTACAGAGAC 
TGTATGACAC AGATGCTGAG GGCAGTGGTG GAGGAAGGGT GCCACAGTCA GAAGAATGTC 
CTCAAGTACC TTGGGGAGCG GTTCCGGGTT AAGTTTTACC TTCCCGACTG GTTTACAGAT 
GAACAAGCTG CAGAGTTCCT TATAAGTCAG TGCATCTGCA TACATCTTAA AACCAATGTA 
GAAAAATTTT ACATGCTCTG TATGATGACA CAAAAGCTGT TCACGTTTGC CAAGGATGGA 
TGCATGGAGG AAAATCCTGA CAGCTTAATG TACCAGGAGG TCATGACACC AGGGCAGCTT 
TTACTGATGT TTTTAAAGGA AAAGATGGAA TCGTGGCTGC TAGGAGTTAA GGTTAATCTG 
GACAAGAGAG CTCATAAATA TACAGTTGAT CTAAGTACTG AAACCATGAT GAAAGTATTT 
ACTTCAACAT CAGATCTAAC AAAAACGTGT GAATATCTGC TGGCTACTGG TAATCTTCGC 
TCTAAAACTG GCCTTGGAAT GCTACAGTCT TCTGGTCTGG TAGTGGTTGC TGACAAGCTA 
AACTTCATTC GCTACCTTTC TCACTTCCGC TGTGTACATA GAGGTGCTGC TTTTGCCAAA 
ATGAGAACCA CCTCAGTGCG TCGCCTTCTG CCTGAGTCTT GGGGTTTCCT GTGCCCCGTA 
CACACTCCCG ATGGTGAACC ATGTGGTCTC ATGAATCATA TGACTGCAGC GTGTGAGATT 
GTGACACAGA CATCCTACAA CACTTTACTG CCATTGCTGC TGTGCTCATT AGGAATGACT 
CCTGTTGATG GAACAGCCGG AAAATTGTTT GCAGACTGCT ACCCTGTCAT GCTAGATGGA 
GCCATGGTGG GTTGGGTGGA GAAAACCATA GCTCCTGGCC TTGTCAGCGC ACTTCGAAAT 
TATAAGGTGA AACATGAAAA GAATGTGCCT CCATGGACTG AAATTGTCCT TATCCCAATG 
ACTGGGAAAG CAAGTCTGTA CCCTGGGCTT TTTCTTTTCA CCACCCCCTG CAGGATGGTA 
CGTCCCGTCA GGAATTTGGC CTTGGGTAAA GAGGAATTGC TTGGCACTTT GGAACAGGTT 
TTCTTGAATG TGGCAGTTTA TGAAGGTGAT ATTGTACCCG GAGAGACCAC TCACCAGGAG 
TTTTTCCCGC ACAGCATGCT GAGTGTAGTA GCAAATTTTA TCCCCTTCTC AGACCACAAC 
CAGAGTCCCA GAAACATGTA TCAGTGTCAG ATGGGTAAGC AGACAATGGG CTTCCCTCTT 
ATGACTTATC AGGATCGCTC TGATAACAAA CTGTACCGGC TACAGACCCC ACAGAGTCCT 
TTAGTTCGGC CTTCCATGTA TGATTATTAT GAGATGGATA ACTATCCCAT TGGCACCAAT 
GCAATCGTAG CAGTCATTTC GTATACAGGG TATGACATGG AGGATGCCAT GGTCTTGAAC 
AAGTCATCAT GGGAAAGAGG CTTTGCTCAT GGAAGTGTGT ATAAAACTGA TCACGTGGAT 
CTCAGTAAAA AACTTAAAGA TGGAGAAGAT AATCTAGTTT TTGGCATCAA ACCAGATGAT 
GAGCGGGTGA AAGGAAGACT GGACAGCGAT GGCCTCCCTG CTGTTGGTGC TGTTCTGCAG 
TTTGGAGACC CTTACTATGG CTACCTGAAC CTTAACACAG GGGAGAGCTT TGTTGCCTAT 
TACAAGAGTA AAGAAAACTG TGTAATTGAC AACATCAAAA TCTGCAGTAA TGACATTGGT 
TTTGGAAAGT TTAAGTCTGT TTGTGTCACC ATGAGAATCC CCCGTAATCC AACAATCGGG 
GATAAATTTG CAAGTCGGCA TGGCCAAAAG GGTATTTTGA GTAGGCTTTG GCCGTCTGAG 
GATATGCCAT TTACAGAGAG TGGAATGGTT CCGGACATCC TTTTCAATCC TCACGGATTT 
CCCTCTAGAA TGACCATTGG GATGCTGATA GAGAGCATGG CTGGAAAGTC AGCAGCCCTG 
CATGGTCTCT GCCACGATGC CACACCCTTT ACATTTTCTG AAGAAAACTC TGCCCTTGAA 
TATTTTGGAG AAATGCTGAG AGCAGGTGGT TACAATTTCT ATGGCACAGA GAAACTATAC 
AGTGGCATAA GTGGCCTGGA ACTTGAAGCC GATATTTTCA TTGGGGTGGT TTACTACCAG 
CGCCTTCGAC ACATGGTCTC TGACAAGTTT CAGGTTAGAA CAACAGGTGC CAGGGATAAA 
GTTACCAACC AGCCTGTGGG AGGCCGTAAT GTTCAAGGTG GGATACGTTT TGGAGAAATG 
GAGCGTGATG CACTGCTGGC CCACGGTACC TCTTTCCTGC TTCATGATCG ACTTTTTAAC 
TGCTCGGATA GGTCGACAGC ACATGTGTGC ATAGAATGTG GAAGCCTACT CTCTCCTTTC 
TTGGAAAAGC CTCCGCCTTC CTGGTCATCC ATACGCAACC GAAAACACCA CTGTGAAGTC 
TGCGATCGTA CGGATACTGT TGAGACTGTA TCAGTACCTT ATGTTTTCCG TTATTTTGTG 
GCTGAATTAG CAGCCATGAA TATCAGTGTG AAACTAAGTG TGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940442.1
CDS	107..3514
Translation	METDTKWQALLREPSLGKLTSPDYGIPGESQHPPLQDITRAHIDSFNQAMTEGLSLAVQAMKPLEFTYKNDKVALQIVDAVITPPLVPKGSICKEQKIYPAECRGRRSTYRGKLTADVSWSLNGLQKGIIKQTLGHIPIMVKSKFCNLYDLNPKQLLEHHEEEQEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKNRGPGYTQYGISLRCVRDEHTAVNMNLHYLENGSVMVNFIYQKELFFIPLGFALKALVDYSDYQIFTELIKGKEENSFYRDCMTQMLRAVVEEGCHSQKNVLKYLGERFRVKFYLPDWFTDEQAAEFLISQCICIHLKTNVEKFYMLCMMTQKLFTFAKDGCMEENPDSLMYQEVMTPGQLLLMFLKEKMESWLLGVKVNLDKRAHKYTVDLSTETMMKVFTSTSDLTKTCEYLLATGNLRSKTGLGMLQSSGLVVVADKLNFIRYLSHFRCVHRGAAFAKMRTTSVRRLLPESWGFLCPVHTPDGEPCGLMNHMTAACEIVTQTSYNTLLPLLLCSLGMTPVDGTAGKLFADCYPVMLDGAMVGWVEKTIAPGLVSALRNYKVKHEKNVPPWTEIVLIPMTGKASLYPGLFLFTTPCRMVRPVRNLALGKEELLGTLEQVFLNVAVYEGDIVPGETTHQEFFPHSMLSVVANFIPFSDHNQSPRNMYQCQMGKQTMGFPLMTYQDRSDNKLYRLQTPQSPLVRPSMYDYYEMDNYPIGTNAIVAVISYTGYDMEDAMVLNKSSWERGFAHGSVYKTDHVDLSKKLKDGEDNLVFGIKPDDERVKGRLDSDGLPAVGAVLQFGDPYYGYLNLNTGESFVAYYKSKENCVIDNIKICSNDIGFGKFKSVCVTMRIPRNPTIGDKFASRHGQKGILSRLWPSEDMPFTESGMVPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFTFSEENSALEYFGEMLRAGGYNFYGTEKLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPVGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSTAHVCIECGSLLSPFLEKPPPSWSSIRNRKHHCEVCDRTDTVETVSVPYVFRYFVAELAAMNISVKLSVS

Target ORF information:

RefSeq Version	XM_002940396.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (RNA) I polypeptide B (polr1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940396.5

ORF Insert Sequence:

ATGGAAACGG ACACTAAGTG GCAAGCGCTG CTCAGGGAGC CTAGCCTGGG CAAACTTACC 
AGTCCCGACT ATGGGATTCC CGGGGAGAGC CAGCACCCTC CTCTGCAGGA TATCACTCGG 
GCTCATATCG ACTCCTTCAA CCAAGCAATG ACAGAGGGGC TGAGTCTGGC GGTGCAGGCT 
ATGAAACCAC TTGAGTTTAC TTACAAAAAT GATAAGGTGG CATTGCAAAT AGTGGATGCA 
GTTATTACCC CTCCTCTAGT GCCCAAAGGA AGCATCTGCA AAGAGCAAAA GATATACCCA 
GCAGAATGCA GAGGAAGAAG AAGTACATAC CGGGGCAAGC TGACTGCCGA TGTAAGCTGG 
TCCTTAAATG GATTGCAAAA AGGCATCATC AAACAAACCC TGGGACATAT TCCTATCATG 
GTGAAGTCAA AATTTTGCAA CCTGTATGAC CTTAACCCAA AACAACTTCT TGAGCACCAC 
GAGGAAGAGC AAGAAATGGG AGGATATTTC ATTATAAATG GAATAGAAAA GGTTATCAGA 
ATGCTTATTA TGCCTCGAAG GAACTTTCCC ATCGCCATGA TTCGTCCCAA GTGGAAGAAT 
AGAGGGCCTG GTTACACTCA GTATGGTATT TCACTTCGTT GTGTCAGAGA TGAACATACT 
GCTGTCAACA TGAACCTGCA CTATCTAGAG AATGGCTCTG TTATGGTGAA TTTCATCTAT 
CAAAAGGAGC TCTTCTTCAT TCCACTGGGC TTTGCATTAA AGGCTCTAGT TGACTATTCA 
GATTATCAGA TTTTCACTGA ATTGATCAAA GGCAAGGAAG AGAATTCATT CTACAGAGAC 
TGTATGACAC AGATGCTGAG GGCAGTGGTG GAGGAAGGGT GCCACAGTCA GAAGAATGTC 
CTCAAGTACC TTGGGGAGCG GTTCCGGGTT AAGTTTTACC TTCCCGACTG GTTTACAGAT 
GAACAAGCTG CAGAGTTCCT TATAAGTCAG TGCATCTGCA TACATCTTAA AACCAATGTA 
GAAAAATTTT ACATGCTCTG TATGATGACA CAAAAGCTGT TCACGTTTGC CAAGGATGGA 
TGCATGGAGG AAAATCCTGA CAGCTTAATG TACCAGGAGG TCATGACACC AGGGCAGCTT 
TTACTGATGT TTTTAAAGGA AAAGATGGAA TCGTGGCTGC TAGGAGTTAA GGTTAATCTG 
GACAAGAGAG CTCATAAATA TACAGTTGAT CTAAGTACTG AAACCATGAT GAAAGTATTT 
ACTTCAACAT CAGATCTAAC AAAAACGTGT GAATATCTGC TGGCTACTGG TAATCTTCGC 
TCTAAAACTG GCCTTGGAAT GCTACAGTCT TCTGGTCTGG TAGTGGTTGC TGACAAGCTA 
AACTTCATTC GCTACCTTTC TCACTTCCGC TGTGTACATA GAGGTGCTGC TTTTGCCAAA 
ATGAGAACCA CCTCAGTGCG TCGCCTTCTG CCTGAGTCTT GGGGTTTCCT GTGCCCCGTA 
CACACTCCCG ATGGTGAACC ATGTGGTCTC ATGAATCATA TGACTGCAGC GTGTGAGATT 
GTGACACAGA CATCCTACAA CACTTTACTG CCATTGCTGC TGTGCTCATT AGGAATGACT 
CCTGTTGATG GAACAGCCGG AAAATTGTTT GCAGACTGCT ACCCTGTCAT GCTAGATGGA 
GCCATGGTGG GTTGGGTGGA GAAAACCATA GCTCCTGGCC TTGTCAGCGC ACTTCGAAAT 
TATAAGGTGA AACATGAAAA GAATGTGCCT CCATGGACTG AAATTGTCCT TATCCCAATG 
ACTGGGAAAG CAAGTCTGTA CCCTGGGCTT TTTCTTTTCA CCACCCCCTG CAGGATGGTA 
CGTCCCGTCA GGAATTTGGC CTTGGGTAAA GAGGAATTGC TTGGCACTTT GGAACAGGTT 
TTCTTGAATG TGGCAGTTTA TGAAGGTGAT ATTGTACCCG GAGAGACCAC TCACCAGGAG 
TTTTTCCCGC ACAGCATGCT GAGTGTAGTA GCAAATTTTA TCCCCTTCTC AGACCACAAC 
CAGAGTCCCA GAAACATGTA TCAGTGTCAG ATGGGTAAGC AGACAATGGG CTTCCCTCTT 
ATGACTTATC AGGATCGCTC TGATAACAAA CTGTACCGGC TACAGACCCC ACAGAGTCCT 
TTAGTTCGGC CTTCCATGTA TGATTATTAT GAGATGGATA ACTATCCCAT TGGCACCAAT 
GCAATCGTAG CAGTCATTTC GTATACAGGG TATGACATGG AGGATGCCAT GGTCTTGAAC 
AAGTCATCAT GGGAAAGAGG CTTTGCTCAT GGAAGTGTGT ATAAAACTGA TCACGTGGAT 
CTCAGTAAAA AACTTAAAGA TGGAGAAGAT AATCTAGTTT TTGGCATCAA ACCAGATGAT 
GAGCGGGTGA AAGGAAGACT GGACAGCGAT GGCCTCCCTG CTGTTGGTGC TGTTCTGCAG 
TTTGGAGACC CTTACTATGG CTACCTGAAC CTTAACACAG GGGAGAGCTT TGTTGCCTAT 
TACAAGAGTA AAGAAAACTG TGTAATTGAC AACATCAAAA TCTGCAGTAA TGACATTGGT 
TTTGGAAAGT TTAAGTCTGT TTGTGTCACC ATGAGAATCC CCCGTAATCC AACAATCGGG 
GATAAATTTG CAAGTCGGCA TGGCCAAAAG GGTATTTTGA GTAGGCTTTG GCCGTCTGAG 
GATATGCCAT TTACAGAGAG TGGAATGGTT CCGGACATCC TTTTCAATCC TCACGGATTT 
CCCTCTAGAA TGACCATTGG GATGCTGATA GAGAGCATGG CTGGAAAGTC AGCAGCCCTG 
CATGGTCTCT GCCACGATGC CACACCCTTT ACATTTTCTG AAGAAAACTC TGCCCTTGAA 
TATTTTGGAG AAATGCTGAG AGCAGGTGGT TACAATTTCT ATGGCACAGA GAAACTATAC 
AGTGGCATAA GTGGCCTGGA ACTTGAAGCC GATATTTTCA TTGGGGTGGT TTACTACCAG 
CGCCTTCGAC ACATGGTCTC TGACAAGTTT CAGGTTAGAA CAACAGGTGC CAGGGATAAA 
GTTACCAACC AGCCTGTGGG AGGCCGTAAT GTTCAAGGTG GGATACGTTT TGGAGAAATG 
GAGCGTGATG CACTGCTGGC CCACGGTACC TCTTTCCTGC TTCATGATCG ACTTTTTAAC 
TGCTCGGATA GGTCGACAGC ACATGTGTGC ATAGAATGTG GAAGCCTACT CTCTCCTTTC 
TTGGAAAAGC CTCCGCCTTC CTGGTCATCC ATACGCAACC GAAAACACCA CTGTGAAGTC 
TGCGATCGTA CGGATACTGT TGAGACTGTA TCAGTACCTT ATGTTTTCCG TTATTTTGTG 
GCTGAATTAG CAGCCATGAA TATCAGTGTG AAACTAAGTG TGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

polr1b ( XM_002940396.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940442.1 Xenopus tropicalis polymerase (RNA) I polypeptide B (polr1b), mRNA.

Price & Availability↑

CloneID	OXa10754
Clone ID Related Accession (Same CDS sequence)	XM_002940396.5 , XM_002940396.4
Accession Version	XM_002940396.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	DNA-directed RNA polymerase I subunit RPA2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940396.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACGG ACACTAAGTG GCAAGCGCTG CTCAGGGAGC CTAGCCTGGG CAAACTTACC 
AGTCCCGACT ATGGGATTCC CGGGGAGAGC CAGCACCCTC CTCTGCAGGA TATCACTCGG 
GCTCATATCG ACTCCTTCAA CCAAGCAATG ACAGAGGGGC TGAGTCTGGC GGTGCAGGCT 
ATGAAACCAC TTGAGTTTAC TTACAAAAAT GATAAGGTGG CATTGCAAAT AGTGGATGCA 
GTTATTACCC CTCCTCTAGT GCCCAAAGGA AGCATCTGCA AAGAGCAAAA GATATACCCA 
GCAGAATGCA GAGGAAGAAG AAGTACATAC CGGGGCAAGC TGACTGCCGA TGTAAGCTGG 
TCCTTAAATG GATTGCAAAA AGGCATCATC AAACAAACCC TGGGACATAT TCCTATCATG 
GTGAAGTCAA AATTTTGCAA CCTGTATGAC CTTAACCCAA AACAACTTCT TGAGCACCAC 
GAGGAAGAGC AAGAAATGGG AGGATATTTC ATTATAAATG GAATAGAAAA GGTTATCAGA 
ATGCTTATTA TGCCTCGAAG GAACTTTCCC ATCGCCATGA TTCGTCCCAA GTGGAAGAAT 
AGAGGGCCTG GTTACACTCA GTATGGTATT TCACTTCGTT GTGTCAGAGA TGAACATACT 
GCTGTCAACA TGAACCTGCA CTATCTAGAG AATGGCTCTG TTATGGTGAA TTTCATCTAT 
CAAAAGGAGC TCTTCTTCAT TCCACTGGGC TTTGCATTAA AGGCTCTAGT TGACTATTCA 
GATTATCAGA TTTTCACTGA ATTGATCAAA GGCAAGGAAG AGAATTCATT CTACAGAGAC 
TGTATGACAC AGATGCTGAG GGCAGTGGTG GAGGAAGGGT GCCACAGTCA GAAGAATGTC 
CTCAAGTACC TTGGGGAGCG GTTCCGGGTT AAGTTTTACC TTCCCGACTG GTTTACAGAT 
GAACAAGCTG CAGAGTTCCT TATAAGTCAG TGCATCTGCA TACATCTTAA AACCAATGTA 
GAAAAATTTT ACATGCTCTG TATGATGACA CAAAAGCTGT TCACGTTTGC CAAGGATGGA 
TGCATGGAGG AAAATCCTGA CAGCTTAATG TACCAGGAGG TCATGACACC AGGGCAGCTT 
TTACTGATGT TTTTAAAGGA AAAGATGGAA TCGTGGCTGC TAGGAGTTAA GGTTAATCTG 
GACAAGAGAG CTCATAAATA TACAGTTGAT CTAAGTACTG AAACCATGAT GAAAGTATTT 
ACTTCAACAT CAGATCTAAC AAAAACGTGT GAATATCTGC TGGCTACTGG TAATCTTCGC 
TCTAAAACTG GCCTTGGAAT GCTACAGTCT TCTGGTCTGG TAGTGGTTGC TGACAAGCTA 
AACTTCATTC GCTACCTTTC TCACTTCCGC TGTGTACATA GAGGTGCTGC TTTTGCCAAA 
ATGAGAACCA CCTCAGTGCG TCGCCTTCTG CCTGAGTCTT GGGGTTTCCT GTGCCCCGTA 
CACACTCCCG ATGGTGAACC ATGTGGTCTC ATGAATCATA TGACTGCAGC GTGTGAGATT 
GTGACACAGA CATCCTACAA CACTTTACTG CCATTGCTGC TGTGCTCATT AGGAATGACT 
CCTGTTGATG GAACAGCCGG AAAATTGTTT GCAGACTGCT ACCCTGTCAT GCTAGATGGA 
GCCATGGTGG GTTGGGTGGA GAAAACCATA GCTCCTGGCC TTGTCAGCGC ACTTCGAAAT 
TATAAGGTGA AACATGAAAA GAATGTGCCT CCATGGACTG AAATTGTCCT TATCCCAATG 
ACTGGGAAAG CAAGTCTGTA CCCTGGGCTT TTTCTTTTCA CCACCCCCTG CAGGATGGTA 
CGTCCCGTCA GGAATTTGGC CTTGGGTAAA GAGGAATTGC TTGGCACTTT GGAACAGGTT 
TTCTTGAATG TGGCAGTTTA TGAAGGTGAT ATTGTACCCG GAGAGACCAC TCACCAGGAG 
TTTTTCCCGC ACAGCATGCT GAGTGTAGTA GCAAATTTTA TCCCCTTCTC AGACCACAAC 
CAGAGTCCCA GAAACATGTA TCAGTGTCAG ATGGGTAAGC AGACAATGGG CTTCCCTCTT 
ATGACTTATC AGGATCGCTC TGATAACAAA CTGTACCGGC TACAGACCCC ACAGAGTCCT 
TTAGTTCGGC CTTCCATGTA TGATTATTAT GAGATGGATA ACTATCCCAT TGGCACCAAT 
GCAATCGTAG CAGTCATTTC GTATACAGGG TATGACATGG AGGATGCCAT GGTCTTGAAC 
AAGTCATCAT GGGAAAGAGG CTTTGCTCAT GGAAGTGTGT ATAAAACTGA TCACGTGGAT 
CTCAGTAAAA AACTTAAAGA TGGAGAAGAT AATCTAGTTT TTGGCATCAA ACCAGATGAT 
GAGCGGGTGA AAGGAAGACT GGACAGCGAT GGCCTCCCTG CTGTTGGTGC TGTTCTGCAG 
TTTGGAGACC CTTACTATGG CTACCTGAAC CTTAACACAG GGGAGAGCTT TGTTGCCTAT 
TACAAGAGTA AAGAAAACTG TGTAATTGAC AACATCAAAA TCTGCAGTAA TGACATTGGT 
TTTGGAAAGT TTAAGTCTGT TTGTGTCACC ATGAGAATCC CCCGTAATCC AACAATCGGG 
GATAAATTTG CAAGTCGGCA TGGCCAAAAG GGTATTTTGA GTAGGCTTTG GCCGTCTGAG 
GATATGCCAT TTACAGAGAG TGGAATGGTT CCGGACATCC TTTTCAATCC TCACGGATTT 
CCCTCTAGAA TGACCATTGG GATGCTGATA GAGAGCATGG CTGGAAAGTC AGCAGCCCTG 
CATGGTCTCT GCCACGATGC CACACCCTTT ACATTTTCTG AAGAAAACTC TGCCCTTGAA 
TATTTTGGAG AAATGCTGAG AGCAGGTGGT TACAATTTCT ATGGCACAGA GAAACTATAC 
AGTGGCATAA GTGGCCTGGA ACTTGAAGCC GATATTTTCA TTGGGGTGGT TTACTACCAG 
CGCCTTCGAC ACATGGTCTC TGACAAGTTT CAGGTTAGAA CAACAGGTGC CAGGGATAAA 
GTTACCAACC AGCCTGTGGG AGGCCGTAAT GTTCAAGGTG GGATACGTTT TGGAGAAATG 
GAGCGTGATG CACTGCTGGC CCACGGTACC TCTTTCCTGC TTCATGATCG ACTTTTTAAC 
TGCTCGGATA GGTCGACAGC ACATGTGTGC ATAGAATGTG GAAGCCTACT CTCTCCTTTC 
TTGGAAAAGC CTCCGCCTTC CTGGTCATCC ATACGCAACC GAAAACACCA CTGTGAAGTC 
TGCGATCGTA CGGATACTGT TGAGACTGTA TCAGTACCTT ATGTTTTCCG TTATTTTGTG 
GCTGAATTAG CAGCCATGAA TATCAGTGTG AAACTAAGTG TGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940442.1
CDS	1..3408
Translation	METDTKWQALLREPSLGKLTSPDYGIPGESQHPPLQDITRAHIDSFNQAMTEGLSLAVQAMKPLEFTYKNDKVALQIVDAVITPPLVPKGSICKEQKIYPAECRGRRSTYRGKLTADVSWSLNGLQKGIIKQTLGHIPIMVKSKFCNLYDLNPKQLLEHHEEEQEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKNRGPGYTQYGISLRCVRDEHTAVNMNLHYLENGSVMVNFIYQKELFFIPLGFALKALVDYSDYQIFTELIKGKEENSFYRDCMTQMLRAVVEEGCHSQKNVLKYLGERFRVKFYLPDWFTDEQAAEFLISQCICIHLKTNVEKFYMLCMMTQKLFTFAKDGCMEENPDSLMYQEVMTPGQLLLMFLKEKMESWLLGVKVNLDKRAHKYTVDLSTETMMKVFTSTSDLTKTCEYLLATGNLRSKTGLGMLQSSGLVVVADKLNFIRYLSHFRCVHRGAAFAKMRTTSVRRLLPESWGFLCPVHTPDGEPCGLMNHMTAACEIVTQTSYNTLLPLLLCSLGMTPVDGTAGKLFADCYPVMLDGAMVGWVEKTIAPGLVSALRNYKVKHEKNVPPWTEIVLIPMTGKASLYPGLFLFTTPCRMVRPVRNLALGKEELLGTLEQVFLNVAVYEGDIVPGETTHQEFFPHSMLSVVANFIPFSDHNQSPRNMYQCQMGKQTMGFPLMTYQDRSDNKLYRLQTPQSPLVRPSMYDYYEMDNYPIGTNAIVAVISYTGYDMEDAMVLNKSSWERGFAHGSVYKTDHVDLSKKLKDGEDNLVFGIKPDDERVKGRLDSDGLPAVGAVLQFGDPYYGYLNLNTGESFVAYYKSKENCVIDNIKICSNDIGFGKFKSVCVTMRIPRNPTIGDKFASRHGQKGILSRLWPSEDMPFTESGMVPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFTFSEENSALEYFGEMLRAGGYNFYGTEKLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDKVTNQPVGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSTAHVCIECGSLLSPFLEKPPPSWSSIRNRKHHCEVCDRTDTVETVSVPYVFRYFVAELAAMNISVKLSVS

Target ORF information:

RefSeq Version	XM_002940396.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis polymerase (RNA) I polypeptide B (polr1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940396.4

ORF Insert Sequence:

ATGGAAACGG ACACTAAGTG GCAAGCGCTG CTCAGGGAGC CTAGCCTGGG CAAACTTACC 
AGTCCCGACT ATGGGATTCC CGGGGAGAGC CAGCACCCTC CTCTGCAGGA TATCACTCGG 
GCTCATATCG ACTCCTTCAA CCAAGCAATG ACAGAGGGGC TGAGTCTGGC GGTGCAGGCT 
ATGAAACCAC TTGAGTTTAC TTACAAAAAT GATAAGGTGG CATTGCAAAT AGTGGATGCA 
GTTATTACCC CTCCTCTAGT GCCCAAAGGA AGCATCTGCA AAGAGCAAAA GATATACCCA 
GCAGAATGCA GAGGAAGAAG AAGTACATAC CGGGGCAAGC TGACTGCCGA TGTAAGCTGG 
TCCTTAAATG GATTGCAAAA AGGCATCATC AAACAAACCC TGGGACATAT TCCTATCATG 
GTGAAGTCAA AATTTTGCAA CCTGTATGAC CTTAACCCAA AACAACTTCT TGAGCACCAC 
GAGGAAGAGC AAGAAATGGG AGGATATTTC ATTATAAATG GAATAGAAAA GGTTATCAGA 
ATGCTTATTA TGCCTCGAAG GAACTTTCCC ATCGCCATGA TTCGTCCCAA GTGGAAGAAT 
AGAGGGCCTG GTTACACTCA GTATGGTATT TCACTTCGTT GTGTCAGAGA TGAACATACT 
GCTGTCAACA TGAACCTGCA CTATCTAGAG AATGGCTCTG TTATGGTGAA TTTCATCTAT 
CAAAAGGAGC TCTTCTTCAT TCCACTGGGC TTTGCATTAA AGGCTCTAGT TGACTATTCA 
GATTATCAGA TTTTCACTGA ATTGATCAAA GGCAAGGAAG AGAATTCATT CTACAGAGAC 
TGTATGACAC AGATGCTGAG GGCAGTGGTG GAGGAAGGGT GCCACAGTCA GAAGAATGTC 
CTCAAGTACC TTGGGGAGCG GTTCCGGGTT AAGTTTTACC TTCCCGACTG GTTTACAGAT 
GAACAAGCTG CAGAGTTCCT TATAAGTCAG TGCATCTGCA TACATCTTAA AACCAATGTA 
GAAAAATTTT ACATGCTCTG TATGATGACA CAAAAGCTGT TCACGTTTGC CAAGGATGGA 
TGCATGGAGG AAAATCCTGA CAGCTTAATG TACCAGGAGG TCATGACACC AGGGCAGCTT 
TTACTGATGT TTTTAAAGGA AAAGATGGAA TCGTGGCTGC TAGGAGTTAA GGTTAATCTG 
GACAAGAGAG CTCATAAATA TACAGTTGAT CTAAGTACTG AAACCATGAT GAAAGTATTT 
ACTTCAACAT CAGATCTAAC AAAAACGTGT GAATATCTGC TGGCTACTGG TAATCTTCGC 
TCTAAAACTG GCCTTGGAAT GCTACAGTCT TCTGGTCTGG TAGTGGTTGC TGACAAGCTA 
AACTTCATTC GCTACCTTTC TCACTTCCGC TGTGTACATA GAGGTGCTGC TTTTGCCAAA 
ATGAGAACCA CCTCAGTGCG TCGCCTTCTG CCTGAGTCTT GGGGTTTCCT GTGCCCCGTA 
CACACTCCCG ATGGTGAACC ATGTGGTCTC ATGAATCATA TGACTGCAGC GTGTGAGATT 
GTGACACAGA CATCCTACAA CACTTTACTG CCATTGCTGC TGTGCTCATT AGGAATGACT 
CCTGTTGATG GAACAGCCGG AAAATTGTTT GCAGACTGCT ACCCTGTCAT GCTAGATGGA 
GCCATGGTGG GTTGGGTGGA GAAAACCATA GCTCCTGGCC TTGTCAGCGC ACTTCGAAAT 
TATAAGGTGA AACATGAAAA GAATGTGCCT CCATGGACTG AAATTGTCCT TATCCCAATG 
ACTGGGAAAG CAAGTCTGTA CCCTGGGCTT TTTCTTTTCA CCACCCCCTG CAGGATGGTA 
CGTCCCGTCA GGAATTTGGC CTTGGGTAAA GAGGAATTGC TTGGCACTTT GGAACAGGTT 
TTCTTGAATG TGGCAGTTTA TGAAGGTGAT ATTGTACCCG GAGAGACCAC TCACCAGGAG 
TTTTTCCCGC ACAGCATGCT GAGTGTAGTA GCAAATTTTA TCCCCTTCTC AGACCACAAC 
CAGAGTCCCA GAAACATGTA TCAGTGTCAG ATGGGTAAGC AGACAATGGG CTTCCCTCTT 
ATGACTTATC AGGATCGCTC TGATAACAAA CTGTACCGGC TACAGACCCC ACAGAGTCCT 
TTAGTTCGGC CTTCCATGTA TGATTATTAT GAGATGGATA ACTATCCCAT TGGCACCAAT 
GCAATCGTAG CAGTCATTTC GTATACAGGG TATGACATGG AGGATGCCAT GGTCTTGAAC 
AAGTCATCAT GGGAAAGAGG CTTTGCTCAT GGAAGTGTGT ATAAAACTGA TCACGTGGAT 
CTCAGTAAAA AACTTAAAGA TGGAGAAGAT AATCTAGTTT TTGGCATCAA ACCAGATGAT 
GAGCGGGTGA AAGGAAGACT GGACAGCGAT GGCCTCCCTG CTGTTGGTGC TGTTCTGCAG 
TTTGGAGACC CTTACTATGG CTACCTGAAC CTTAACACAG GGGAGAGCTT TGTTGCCTAT 
TACAAGAGTA AAGAAAACTG TGTAATTGAC AACATCAAAA TCTGCAGTAA TGACATTGGT 
TTTGGAAAGT TTAAGTCTGT TTGTGTCACC ATGAGAATCC CCCGTAATCC AACAATCGGG 
GATAAATTTG CAAGTCGGCA TGGCCAAAAG GGTATTTTGA GTAGGCTTTG GCCGTCTGAG 
GATATGCCAT TTACAGAGAG TGGAATGGTT CCGGACATCC TTTTCAATCC TCACGGATTT 
CCCTCTAGAA TGACCATTGG GATGCTGATA GAGAGCATGG CTGGAAAGTC AGCAGCCCTG 
CATGGTCTCT GCCACGATGC CACACCCTTT ACATTTTCTG AAGAAAACTC TGCCCTTGAA 
TATTTTGGAG AAATGCTGAG AGCAGGTGGT TACAATTTCT ATGGCACAGA GAAACTATAC 
AGTGGCATAA GTGGCCTGGA ACTTGAAGCC GATATTTTCA TTGGGGTGGT TTACTACCAG 
CGCCTTCGAC ACATGGTCTC TGACAAGTTT CAGGTTAGAA CAACAGGTGC CAGGGATAAA 
GTTACCAACC AGCCTGTGGG AGGCCGTAAT GTTCAAGGTG GGATACGTTT TGGAGAAATG 
GAGCGTGATG CACTGCTGGC CCACGGTACC TCTTTCCTGC TTCATGATCG ACTTTTTAAC 
TGCTCGGATA GGTCGACAGC ACATGTGTGC ATAGAATGTG GAAGCCTACT CTCTCCTTTC 
TTGGAAAAGC CTCCGCCTTC CTGGTCATCC ATACGCAACC GAAAACACCA CTGTGAAGTC 
TGCGATCGTA CGGATACTGT TGAGACTGTA TCAGTACCTT ATGTTTTCCG TTATTTTGTG 
GCTGAATTAG CAGCCATGAA TATCAGTGTG AAACTAAGTG TGAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

polr1b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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