grm5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	grm5
Entrez Gene ID	100485273
Full Name	glutamate receptor, metabotropic 5
General protein information	Preferred Names glutamate receptor, metabotropic 5 Names metabotropic glutamate receptor 5
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018091755.1	XP_017947244.1	metabotropic glutamate receptor 5 isoform X1
XM_012958034.3	XP_012813488.1	metabotropic glutamate receptor 5 isoform X3
XM_012958034.2	XP_012813488.1	metabotropic glutamate receptor 5 isoform X3
XM_012958033.3	XP_012813487.1	metabotropic glutamate receptor 5 isoform X2
XM_018091755.2	XP_017947244.1	metabotropic glutamate receptor 5 isoform X1
XM_018091756.1	XP_017947245.1	metabotropic glutamate receptor 5 isoform X1
XM_012958033.2	XP_012813487.1	metabotropic glutamate receptor 5 isoform X2
XM_018091756.2	XP_017947245.1	metabotropic glutamate receptor 5 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GRM5	XP_001135787.2
Canis lupus familiaris (dog)	GRM5	XP_005633436.1
Bos taurus (cattle)	GRM5	XP_003588057.1
Mus musculus (house mouse)	Grm5	NP_001137306.1
Rattus norvegicus (Norway rat)	Grm5	NP_058708.1
Gallus gallus (chicken)	GRM5	NP_989469.1
Homo sapiens (human)	GRM5	NP_001137303.1
Xenopus tropicalis (tropical clawed frog)	grm5	XP_002936597.1
	GRM5	XP_002808131.1
Danio rerio (zebrafish)	LOC100332913	XP_002664765.2

Gene Ontology

The following grm5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the grm5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa06465	XM_012958033.3 Latest version!	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa06466	XM_012958034.3 Latest version!	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa06461	XM_018091756.2 Latest version!	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa06461	XM_018091755.2 Latest version!	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OXa06466	XM_012958034.2	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa06461	XM_018091755.1	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OXa06465	XM_012958033.2	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa06461	XM_018091756.1	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

grm5 ( XM_012958033.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813487.1 Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa06465
Clone ID Related Accession (Same CDS sequence)	XM_012958033.3 , XM_012958033.2
Accession Version	XM_012958033.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	metabotropic glutamate receptor 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958033.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGTC GTCCCATGTC CTTCATCAGA CCACGTGTTC CAGATCATTT CATTTACTCC 
TGGGCCTACC CACTCCTATT GTCAGCAGCC AATCGGAATC TCAAATGGCA AATCTGTTAC 
ATGGGCGCAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813487.1
CDS	756..3530
Translation	MVLHFVLLLLLLRDDGNGNVWIMASAQTNERRVVSYMAGDIIIGALFSVHHQPTVDKVHERKCGEIREQYGIQRVEAMLHALDRINADQTLLPNISLGCEIRDSCWHTAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSAPSRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLYKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGERGMEAFKDMSAKDGICIAHSYKIYSNAGEQSFDKLLKKLRSHLPKARVVVCFCEGMTVRGLLMAMKRQNIIGEFLLLGSDGWADRYDVTDGYQREAAGGITIKLQSPDVKWFDDYYLQLRPENNLRNPWFQEFWQHRFQCKLKGYSQENQKYNRTCNSRMSLRSQYVQDSKMGFVINAIYAMAYGLHNMQTVLCPGYAGLCDAMKPIDGQKLLDSIMKANFTGVSGDMITFDENGDSPGRYEIMNFKKMGKDYYDYINVGSWDNGELKMDDDEIWSNKNLVIRSVCSDPCAKGQIKVIRKGEVSCCWTCTPCKDNEIVFDEYTCKACDLGSWPTDDLTGCDPIPVEFLRWSDPEPIAAVVFSCLGLLATMFVMAIFILYRDTPVVKSSSRELCYIILAGICLGYLCTFSLIAKPQQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFVLICIQLGIIVALFIMEPPDVIFDYPSIREVYLICNTNNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTSTVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLSRPMSFIRPRVPDHFIYSWAYPLLLSAANRNLKWQICYMGAE

Target ORF information:

RefSeq Version	XM_012958033.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958033.2

ORF Insert Sequence:

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGTC GTCCCATGTC CTTCATCAGA CCACGTGTTC CAGATCATTT CATTTACTCC 
TGGGCCTACC CACTCCTATT GTCAGCAGCC AATCGGAATC TCAAATGGCA AATCTGTTAC 
ATGGGCGCAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grm5 ( XM_012958034.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813488.1 Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa06466
Clone ID Related Accession (Same CDS sequence)	XM_012958034.3 , XM_012958034.2
Accession Version	XM_012958034.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	metabotropic glutamate receptor 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012958034.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGTC GTCCCATGTC CTTCATCAGA CCACGTGTTC CAGATCATTT CATTTACTCC 
TGGGCCTACC CACTCCTATT GTCAGCAGCC AATCGGAATA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813488.1
CDS	756..3500
Translation	MVLHFVLLLLLLRDDGNGNVWIMASAQTNERRVVSYMAGDIIIGALFSVHHQPTVDKVHERKCGEIREQYGIQRVEAMLHALDRINADQTLLPNISLGCEIRDSCWHTAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSAPSRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLYKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGERGMEAFKDMSAKDGICIAHSYKIYSNAGEQSFDKLLKKLRSHLPKARVVVCFCEGMTVRGLLMAMKRQNIIGEFLLLGSDGWADRYDVTDGYQREAAGGITIKLQSPDVKWFDDYYLQLRPENNLRNPWFQEFWQHRFQCKLKGYSQENQKYNRTCNSRMSLRSQYVQDSKMGFVINAIYAMAYGLHNMQTVLCPGYAGLCDAMKPIDGQKLLDSIMKANFTGVSGDMITFDENGDSPGRYEIMNFKKMGKDYYDYINVGSWDNGELKMDDDEIWSNKNLVIRSVCSDPCAKGQIKVIRKGEVSCCWTCTPCKDNEIVFDEYTCKACDLGSWPTDDLTGCDPIPVEFLRWSDPEPIAAVVFSCLGLLATMFVMAIFILYRDTPVVKSSSRELCYIILAGICLGYLCTFSLIAKPQQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFVLICIQLGIIVALFIMEPPDVIFDYPSIREVYLICNTNNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTSTVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLSRPMSFIRPRVPDHFIYSWAYPLLLSAANRNK

Target ORF information:

RefSeq Version	XM_012958034.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958034.2

ORF Insert Sequence:

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGTC GTCCCATGTC CTTCATCAGA CCACGTGTTC CAGATCATTT CATTTACTCC 
TGGGCCTACC CACTCCTATT GTCAGCAGCC AATCGGAATA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grm5 ( XM_012958033.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813487.1 Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa06465
Clone ID Related Accession (Same CDS sequence)	XM_012958033.3 , XM_012958033.2
Accession Version	XM_012958033.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	metabotropic glutamate receptor 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958033.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGTC GTCCCATGTC CTTCATCAGA CCACGTGTTC CAGATCATTT CATTTACTCC 
TGGGCCTACC CACTCCTATT GTCAGCAGCC AATCGGAATC TCAAATGGCA AATCTGTTAC 
ATGGGCGCAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813487.1
CDS	849..3623
Translation	MVLHFVLLLLLLRDDGNGNVWIMASAQTNERRVVSYMAGDIIIGALFSVHHQPTVDKVHERKCGEIREQYGIQRVEAMLHALDRINADQTLLPNISLGCEIRDSCWHTAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSAPSRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLYKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGERGMEAFKDMSAKDGICIAHSYKIYSNAGEQSFDKLLKKLRSHLPKARVVVCFCEGMTVRGLLMAMKRQNIIGEFLLLGSDGWADRYDVTDGYQREAAGGITIKLQSPDVKWFDDYYLQLRPENNLRNPWFQEFWQHRFQCKLKGYSQENQKYNRTCNSRMSLRSQYVQDSKMGFVINAIYAMAYGLHNMQTVLCPGYAGLCDAMKPIDGQKLLDSIMKANFTGVSGDMITFDENGDSPGRYEIMNFKKMGKDYYDYINVGSWDNGELKMDDDEIWSNKNLVIRSVCSDPCAKGQIKVIRKGEVSCCWTCTPCKDNEIVFDEYTCKACDLGSWPTDDLTGCDPIPVEFLRWSDPEPIAAVVFSCLGLLATMFVMAIFILYRDTPVVKSSSRELCYIILAGICLGYLCTFSLIAKPQQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFVLICIQLGIIVALFIMEPPDVIFDYPSIREVYLICNTNNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTSTVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLSRPMSFIRPRVPDHFIYSWAYPLLLSAANRNLKWQICYMGAE

Target ORF information:

RefSeq Version	XM_012958033.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958033.3

ORF Insert Sequence:

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGTC GTCCCATGTC CTTCATCAGA CCACGTGTTC CAGATCATTT CATTTACTCC 
TGGGCCTACC CACTCCTATT GTCAGCAGCC AATCGGAATC TCAAATGGCA AATCTGTTAC 
ATGGGCGCAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grm5 ( XM_012958034.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012813488.1 Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa06466
Clone ID Related Accession (Same CDS sequence)	XM_012958034.3 , XM_012958034.2
Accession Version	XM_012958034.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	metabotropic glutamate receptor 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012958034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGTC GTCCCATGTC CTTCATCAGA CCACGTGTTC CAGATCATTT CATTTACTCC 
TGGGCCTACC CACTCCTATT GTCAGCAGCC AATCGGAATA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012813488.1
CDS	849..3593
Translation	MVLHFVLLLLLLRDDGNGNVWIMASAQTNERRVVSYMAGDIIIGALFSVHHQPTVDKVHERKCGEIREQYGIQRVEAMLHALDRINADQTLLPNISLGCEIRDSCWHTAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSAPSRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLYKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGERGMEAFKDMSAKDGICIAHSYKIYSNAGEQSFDKLLKKLRSHLPKARVVVCFCEGMTVRGLLMAMKRQNIIGEFLLLGSDGWADRYDVTDGYQREAAGGITIKLQSPDVKWFDDYYLQLRPENNLRNPWFQEFWQHRFQCKLKGYSQENQKYNRTCNSRMSLRSQYVQDSKMGFVINAIYAMAYGLHNMQTVLCPGYAGLCDAMKPIDGQKLLDSIMKANFTGVSGDMITFDENGDSPGRYEIMNFKKMGKDYYDYINVGSWDNGELKMDDDEIWSNKNLVIRSVCSDPCAKGQIKVIRKGEVSCCWTCTPCKDNEIVFDEYTCKACDLGSWPTDDLTGCDPIPVEFLRWSDPEPIAAVVFSCLGLLATMFVMAIFILYRDTPVVKSSSRELCYIILAGICLGYLCTFSLIAKPQQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFVLICIQLGIIVALFIMEPPDVIFDYPSIREVYLICNTNNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTSTVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLSRPMSFIRPRVPDHFIYSWAYPLLLSAANRNK

Target ORF information:

RefSeq Version	XM_012958034.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012958034.3

ORF Insert Sequence:

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGTC GTCCCATGTC CTTCATCAGA CCACGTGTTC CAGATCATTT CATTTACTCC 
TGGGCCTACC CACTCCTATT GTCAGCAGCC AATCGGAATA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grm5 ( XM_018091755.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947244.1 Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X1, mRNA.

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CloneID	OXa06461
Clone ID Related Accession (Same CDS sequence)	XM_018091755.1 , XM_018091755.2 , XM_018091756.1 , XM_018091756.2
Accession Version	XM_018091755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	metabotropic glutamate receptor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091755.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGCA TTTCATTTAC TCCTGGGCCT ACCCACTCCT ATTGTCAGCA GCCAATCGGA 
ATCTCAAATG GCAAATCTGT TACATGGGCG CAGAATGAGA AAAGCAGTAA AAGTCAACAT 
CTCTGGCAGA GACTCTCGGT TCACGTCAAG AAGAAGGATA GCACCAATCA AACGGCGGTC 
ATTAAGCCTT TTTCAAAAAG CAGCAGTGAG AACAGATACA TCAGCACATC TTTCCCCGAC 
AGCAGTGCCA AAATGTTATA TGATGTTTCA GAGGCGGAAG AGCATTATCC AGTGCAGTAC 
CGACCGCAGA CTCCTTCGCC AATCAGTACT GTAAGCCAGA GAGCTATTTC AACAACCCGC 
ACAGAGGATG ATATTCCTTC TTTTCATTCG GATACGGCCC AAAGGAGTGG CTCCTCGCAA 
GGTTCCCTAA TGGACCAAAT CAGCAGCGTG GTGACGCGTT TCACCGCTAA CATCAGTGAG 
CTAAATTCAA TGATGCTATC TGCAAATACG CCAGGAACAA TGGTGCCCAC GCCTCTTTGT 
TCTTCCTACC TGATCCCGAG AGAGATTCAA CTGCCCACAA CAATGACCAC CTTTGCGGAG 
ATCCAACCTG CCCCTAACAT TGATTTCAAC GGCGGGACGC AGTCCGTCAG AAAATCAACG 
GACAGCGTCA AAGATAGCAC TTCAGAAGCG GCAGCAGTAA AGCAAGACGT CGAGGAGTTG 
GTAGCTTTAA CGCCACCTTC TCCTTTTAGA GACTCCATTG ACTCCGAAAG CACTTCCCCT 
AGTTCACCAG TTTCGGAATC GGCTCTCTGT ATTCCATCCT CTCCCAAATA TGACAATATG 
ATCATGAGAG ATTACTCCCA GAGCTCCTCC TCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947244.1
CDS	849..4367
Translation	MVLHFVLLLLLLRDDGNGNVWIMASAQTNERRVVSYMAGDIIIGALFSVHHQPTVDKVHERKCGEIREQYGIQRVEAMLHALDRINADQTLLPNISLGCEIRDSCWHTAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSAPSRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLYKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGERGMEAFKDMSAKDGICIAHSYKIYSNAGEQSFDKLLKKLRSHLPKARVVVCFCEGMTVRGLLMAMKRQNIIGEFLLLGSDGWADRYDVTDGYQREAAGGITIKLQSPDVKWFDDYYLQLRPENNLRNPWFQEFWQHRFQCKLKGYSQENQKYNRTCNSRMSLRSQYVQDSKMGFVINAIYAMAYGLHNMQTVLCPGYAGLCDAMKPIDGQKLLDSIMKANFTGVSGDMITFDENGDSPGRYEIMNFKKMGKDYYDYINVGSWDNGELKMDDDEIWSNKNLVIRSVCSDPCAKGQIKVIRKGEVSCCWTCTPCKDNEIVFDEYTCKACDLGSWPTDDLTGCDPIPVEFLRWSDPEPIAAVVFSCLGLLATMFVMAIFILYRDTPVVKSSSRELCYIILAGICLGYLCTFSLIAKPQQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFVLICIQLGIIVALFIMEPPDVIFDYPSIREVYLICNTNNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTSTVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLSISFTPGPTHSYCQQPIGISNGKSVTWAQNEKSSKSQHLWQRLSVHVKKKDSTNQTAVIKPFSKSSSENRYISTSFPDSSAKMLYDVSEAEEHYPVQYRPQTPSPISTVSQRAISTTRTEDDIPSFHSDTAQRSGSSQGSLMDQISSVVTRFTANISELNSMMLSANTPGTMVPTPLCSSYLIPREIQLPTTMTTFAEIQPAPNIDFNGGTQSVRKSTDSVKDSTSEAAAVKQDVEELVALTPPSPFRDSIDSESTSPSSPVSESALCIPSSPKYDNMIMRDYSQSSSSL

Target ORF information:

RefSeq Version	XM_018091755.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091755.2

ORF Insert Sequence:

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGCA TTTCATTTAC TCCTGGGCCT ACCCACTCCT ATTGTCAGCA GCCAATCGGA 
ATCTCAAATG GCAAATCTGT TACATGGGCG CAGAATGAGA AAAGCAGTAA AAGTCAACAT 
CTCTGGCAGA GACTCTCGGT TCACGTCAAG AAGAAGGATA GCACCAATCA AACGGCGGTC 
ATTAAGCCTT TTTCAAAAAG CAGCAGTGAG AACAGATACA TCAGCACATC TTTCCCCGAC 
AGCAGTGCCA AAATGTTATA TGATGTTTCA GAGGCGGAAG AGCATTATCC AGTGCAGTAC 
CGACCGCAGA CTCCTTCGCC AATCAGTACT GTAAGCCAGA GAGCTATTTC AACAACCCGC 
ACAGAGGATG ATATTCCTTC TTTTCATTCG GATACGGCCC AAAGGAGTGG CTCCTCGCAA 
GGTTCCCTAA TGGACCAAAT CAGCAGCGTG GTGACGCGTT TCACCGCTAA CATCAGTGAG 
CTAAATTCAA TGATGCTATC TGCAAATACG CCAGGAACAA TGGTGCCCAC GCCTCTTTGT 
TCTTCCTACC TGATCCCGAG AGAGATTCAA CTGCCCACAA CAATGACCAC CTTTGCGGAG 
ATCCAACCTG CCCCTAACAT TGATTTCAAC GGCGGGACGC AGTCCGTCAG AAAATCAACG 
GACAGCGTCA AAGATAGCAC TTCAGAAGCG GCAGCAGTAA AGCAAGACGT CGAGGAGTTG 
GTAGCTTTAA CGCCACCTTC TCCTTTTAGA GACTCCATTG ACTCCGAAAG CACTTCCCCT 
AGTTCACCAG TTTCGGAATC GGCTCTCTGT ATTCCATCCT CTCCCAAATA TGACAATATG 
ATCATGAGAG ATTACTCCCA GAGCTCCTCC TCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grm5 ( XM_018091756.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947245.1 Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa06461
Clone ID Related Accession (Same CDS sequence)	XM_018091755.1 , XM_018091755.2 , XM_018091756.1 , XM_018091756.2
Accession Version	XM_018091756.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	metabotropic glutamate receptor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGCA TTTCATTTAC TCCTGGGCCT ACCCACTCCT ATTGTCAGCA GCCAATCGGA 
ATCTCAAATG GCAAATCTGT TACATGGGCG CAGAATGAGA AAAGCAGTAA AAGTCAACAT 
CTCTGGCAGA GACTCTCGGT TCACGTCAAG AAGAAGGATA GCACCAATCA AACGGCGGTC 
ATTAAGCCTT TTTCAAAAAG CAGCAGTGAG AACAGATACA TCAGCACATC TTTCCCCGAC 
AGCAGTGCCA AAATGTTATA TGATGTTTCA GAGGCGGAAG AGCATTATCC AGTGCAGTAC 
CGACCGCAGA CTCCTTCGCC AATCAGTACT GTAAGCCAGA GAGCTATTTC AACAACCCGC 
ACAGAGGATG ATATTCCTTC TTTTCATTCG GATACGGCCC AAAGGAGTGG CTCCTCGCAA 
GGTTCCCTAA TGGACCAAAT CAGCAGCGTG GTGACGCGTT TCACCGCTAA CATCAGTGAG 
CTAAATTCAA TGATGCTATC TGCAAATACG CCAGGAACAA TGGTGCCCAC GCCTCTTTGT 
TCTTCCTACC TGATCCCGAG AGAGATTCAA CTGCCCACAA CAATGACCAC CTTTGCGGAG 
ATCCAACCTG CCCCTAACAT TGATTTCAAC GGCGGGACGC AGTCCGTCAG AAAATCAACG 
GACAGCGTCA AAGATAGCAC TTCAGAAGCG GCAGCAGTAA AGCAAGACGT CGAGGAGTTG 
GTAGCTTTAA CGCCACCTTC TCCTTTTAGA GACTCCATTG ACTCCGAAAG CACTTCCCCT 
AGTTCACCAG TTTCGGAATC GGCTCTCTGT ATTCCATCCT CTCCCAAATA TGACAATATG 
ATCATGAGAG ATTACTCCCA GAGCTCCTCC TCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947245.1
CDS	725..4243
Translation	MVLHFVLLLLLLRDDGNGNVWIMASAQTNERRVVSYMAGDIIIGALFSVHHQPTVDKVHERKCGEIREQYGIQRVEAMLHALDRINADQTLLPNISLGCEIRDSCWHTAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSAPSRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLYKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGERGMEAFKDMSAKDGICIAHSYKIYSNAGEQSFDKLLKKLRSHLPKARVVVCFCEGMTVRGLLMAMKRQNIIGEFLLLGSDGWADRYDVTDGYQREAAGGITIKLQSPDVKWFDDYYLQLRPENNLRNPWFQEFWQHRFQCKLKGYSQENQKYNRTCNSRMSLRSQYVQDSKMGFVINAIYAMAYGLHNMQTVLCPGYAGLCDAMKPIDGQKLLDSIMKANFTGVSGDMITFDENGDSPGRYEIMNFKKMGKDYYDYINVGSWDNGELKMDDDEIWSNKNLVIRSVCSDPCAKGQIKVIRKGEVSCCWTCTPCKDNEIVFDEYTCKACDLGSWPTDDLTGCDPIPVEFLRWSDPEPIAAVVFSCLGLLATMFVMAIFILYRDTPVVKSSSRELCYIILAGICLGYLCTFSLIAKPQQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFVLICIQLGIIVALFIMEPPDVIFDYPSIREVYLICNTNNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTSTVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLSISFTPGPTHSYCQQPIGISNGKSVTWAQNEKSSKSQHLWQRLSVHVKKKDSTNQTAVIKPFSKSSSENRYISTSFPDSSAKMLYDVSEAEEHYPVQYRPQTPSPISTVSQRAISTTRTEDDIPSFHSDTAQRSGSSQGSLMDQISSVVTRFTANISELNSMMLSANTPGTMVPTPLCSSYLIPREIQLPTTMTTFAEIQPAPNIDFNGGTQSVRKSTDSVKDSTSEAAAVKQDVEELVALTPPSPFRDSIDSESTSPSSPVSESALCIPSSPKYDNMIMRDYSQSSSSL

Target ORF information:

RefSeq Version	XM_018091756.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091756.2

ORF Insert Sequence:

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGCA TTTCATTTAC TCCTGGGCCT ACCCACTCCT ATTGTCAGCA GCCAATCGGA 
ATCTCAAATG GCAAATCTGT TACATGGGCG CAGAATGAGA AAAGCAGTAA AAGTCAACAT 
CTCTGGCAGA GACTCTCGGT TCACGTCAAG AAGAAGGATA GCACCAATCA AACGGCGGTC 
ATTAAGCCTT TTTCAAAAAG CAGCAGTGAG AACAGATACA TCAGCACATC TTTCCCCGAC 
AGCAGTGCCA AAATGTTATA TGATGTTTCA GAGGCGGAAG AGCATTATCC AGTGCAGTAC 
CGACCGCAGA CTCCTTCGCC AATCAGTACT GTAAGCCAGA GAGCTATTTC AACAACCCGC 
ACAGAGGATG ATATTCCTTC TTTTCATTCG GATACGGCCC AAAGGAGTGG CTCCTCGCAA 
GGTTCCCTAA TGGACCAAAT CAGCAGCGTG GTGACGCGTT TCACCGCTAA CATCAGTGAG 
CTAAATTCAA TGATGCTATC TGCAAATACG CCAGGAACAA TGGTGCCCAC GCCTCTTTGT 
TCTTCCTACC TGATCCCGAG AGAGATTCAA CTGCCCACAA CAATGACCAC CTTTGCGGAG 
ATCCAACCTG CCCCTAACAT TGATTTCAAC GGCGGGACGC AGTCCGTCAG AAAATCAACG 
GACAGCGTCA AAGATAGCAC TTCAGAAGCG GCAGCAGTAA AGCAAGACGT CGAGGAGTTG 
GTAGCTTTAA CGCCACCTTC TCCTTTTAGA GACTCCATTG ACTCCGAAAG CACTTCCCCT 
AGTTCACCAG TTTCGGAATC GGCTCTCTGT ATTCCATCCT CTCCCAAATA TGACAATATG 
ATCATGAGAG ATTACTCCCA GAGCTCCTCC TCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grm5 ( XM_018091755.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947244.1 Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X1, mRNA.

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CloneID	OXa06461
Clone ID Related Accession (Same CDS sequence)	XM_018091755.1 , XM_018091755.2 , XM_018091756.1 , XM_018091756.2
Accession Version	XM_018091755.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	metabotropic glutamate receptor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGCA TTTCATTTAC TCCTGGGCCT ACCCACTCCT ATTGTCAGCA GCCAATCGGA 
ATCTCAAATG GCAAATCTGT TACATGGGCG CAGAATGAGA AAAGCAGTAA AAGTCAACAT 
CTCTGGCAGA GACTCTCGGT TCACGTCAAG AAGAAGGATA GCACCAATCA AACGGCGGTC 
ATTAAGCCTT TTTCAAAAAG CAGCAGTGAG AACAGATACA TCAGCACATC TTTCCCCGAC 
AGCAGTGCCA AAATGTTATA TGATGTTTCA GAGGCGGAAG AGCATTATCC AGTGCAGTAC 
CGACCGCAGA CTCCTTCGCC AATCAGTACT GTAAGCCAGA GAGCTATTTC AACAACCCGC 
ACAGAGGATG ATATTCCTTC TTTTCATTCG GATACGGCCC AAAGGAGTGG CTCCTCGCAA 
GGTTCCCTAA TGGACCAAAT CAGCAGCGTG GTGACGCGTT TCACCGCTAA CATCAGTGAG 
CTAAATTCAA TGATGCTATC TGCAAATACG CCAGGAACAA TGGTGCCCAC GCCTCTTTGT 
TCTTCCTACC TGATCCCGAG AGAGATTCAA CTGCCCACAA CAATGACCAC CTTTGCGGAG 
ATCCAACCTG CCCCTAACAT TGATTTCAAC GGCGGGACGC AGTCCGTCAG AAAATCAACG 
GACAGCGTCA AAGATAGCAC TTCAGAAGCG GCAGCAGTAA AGCAAGACGT CGAGGAGTTG 
GTAGCTTTAA CGCCACCTTC TCCTTTTAGA GACTCCATTG ACTCCGAAAG CACTTCCCCT 
AGTTCACCAG TTTCGGAATC GGCTCTCTGT ATTCCATCCT CTCCCAAATA TGACAATATG 
ATCATGAGAG ATTACTCCCA GAGCTCCTCC TCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947244.1
CDS	756..4274
Translation	MVLHFVLLLLLLRDDGNGNVWIMASAQTNERRVVSYMAGDIIIGALFSVHHQPTVDKVHERKCGEIREQYGIQRVEAMLHALDRINADQTLLPNISLGCEIRDSCWHTAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSAPSRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLYKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGERGMEAFKDMSAKDGICIAHSYKIYSNAGEQSFDKLLKKLRSHLPKARVVVCFCEGMTVRGLLMAMKRQNIIGEFLLLGSDGWADRYDVTDGYQREAAGGITIKLQSPDVKWFDDYYLQLRPENNLRNPWFQEFWQHRFQCKLKGYSQENQKYNRTCNSRMSLRSQYVQDSKMGFVINAIYAMAYGLHNMQTVLCPGYAGLCDAMKPIDGQKLLDSIMKANFTGVSGDMITFDENGDSPGRYEIMNFKKMGKDYYDYINVGSWDNGELKMDDDEIWSNKNLVIRSVCSDPCAKGQIKVIRKGEVSCCWTCTPCKDNEIVFDEYTCKACDLGSWPTDDLTGCDPIPVEFLRWSDPEPIAAVVFSCLGLLATMFVMAIFILYRDTPVVKSSSRELCYIILAGICLGYLCTFSLIAKPQQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFVLICIQLGIIVALFIMEPPDVIFDYPSIREVYLICNTNNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTSTVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLSISFTPGPTHSYCQQPIGISNGKSVTWAQNEKSSKSQHLWQRLSVHVKKKDSTNQTAVIKPFSKSSSENRYISTSFPDSSAKMLYDVSEAEEHYPVQYRPQTPSPISTVSQRAISTTRTEDDIPSFHSDTAQRSGSSQGSLMDQISSVVTRFTANISELNSMMLSANTPGTMVPTPLCSSYLIPREIQLPTTMTTFAEIQPAPNIDFNGGTQSVRKSTDSVKDSTSEAAAVKQDVEELVALTPPSPFRDSIDSESTSPSSPVSESALCIPSSPKYDNMIMRDYSQSSSSL

Target ORF information:

RefSeq Version	XM_018091755.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091755.1

ORF Insert Sequence:

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGCA TTTCATTTAC TCCTGGGCCT ACCCACTCCT ATTGTCAGCA GCCAATCGGA 
ATCTCAAATG GCAAATCTGT TACATGGGCG CAGAATGAGA AAAGCAGTAA AAGTCAACAT 
CTCTGGCAGA GACTCTCGGT TCACGTCAAG AAGAAGGATA GCACCAATCA AACGGCGGTC 
ATTAAGCCTT TTTCAAAAAG CAGCAGTGAG AACAGATACA TCAGCACATC TTTCCCCGAC 
AGCAGTGCCA AAATGTTATA TGATGTTTCA GAGGCGGAAG AGCATTATCC AGTGCAGTAC 
CGACCGCAGA CTCCTTCGCC AATCAGTACT GTAAGCCAGA GAGCTATTTC AACAACCCGC 
ACAGAGGATG ATATTCCTTC TTTTCATTCG GATACGGCCC AAAGGAGTGG CTCCTCGCAA 
GGTTCCCTAA TGGACCAAAT CAGCAGCGTG GTGACGCGTT TCACCGCTAA CATCAGTGAG 
CTAAATTCAA TGATGCTATC TGCAAATACG CCAGGAACAA TGGTGCCCAC GCCTCTTTGT 
TCTTCCTACC TGATCCCGAG AGAGATTCAA CTGCCCACAA CAATGACCAC CTTTGCGGAG 
ATCCAACCTG CCCCTAACAT TGATTTCAAC GGCGGGACGC AGTCCGTCAG AAAATCAACG 
GACAGCGTCA AAGATAGCAC TTCAGAAGCG GCAGCAGTAA AGCAAGACGT CGAGGAGTTG 
GTAGCTTTAA CGCCACCTTC TCCTTTTAGA GACTCCATTG ACTCCGAAAG CACTTCCCCT 
AGTTCACCAG TTTCGGAATC GGCTCTCTGT ATTCCATCCT CTCCCAAATA TGACAATATG 
ATCATGAGAG ATTACTCCCA GAGCTCCTCC TCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

grm5 ( XM_018091756.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947245.1 Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X2, mRNA.

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CloneID	OXa06461
Clone ID Related Accession (Same CDS sequence)	XM_018091755.1 , XM_018091755.2 , XM_018091756.1 , XM_018091756.2
Accession Version	XM_018091756.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	metabotropic glutamate receptor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGCA TTTCATTTAC TCCTGGGCCT ACCCACTCCT ATTGTCAGCA GCCAATCGGA 
ATCTCAAATG GCAAATCTGT TACATGGGCG CAGAATGAGA AAAGCAGTAA AAGTCAACAT 
CTCTGGCAGA GACTCTCGGT TCACGTCAAG AAGAAGGATA GCACCAATCA AACGGCGGTC 
ATTAAGCCTT TTTCAAAAAG CAGCAGTGAG AACAGATACA TCAGCACATC TTTCCCCGAC 
AGCAGTGCCA AAATGTTATA TGATGTTTCA GAGGCGGAAG AGCATTATCC AGTGCAGTAC 
CGACCGCAGA CTCCTTCGCC AATCAGTACT GTAAGCCAGA GAGCTATTTC AACAACCCGC 
ACAGAGGATG ATATTCCTTC TTTTCATTCG GATACGGCCC AAAGGAGTGG CTCCTCGCAA 
GGTTCCCTAA TGGACCAAAT CAGCAGCGTG GTGACGCGTT TCACCGCTAA CATCAGTGAG 
CTAAATTCAA TGATGCTATC TGCAAATACG CCAGGAACAA TGGTGCCCAC GCCTCTTTGT 
TCTTCCTACC TGATCCCGAG AGAGATTCAA CTGCCCACAA CAATGACCAC CTTTGCGGAG 
ATCCAACCTG CCCCTAACAT TGATTTCAAC GGCGGGACGC AGTCCGTCAG AAAATCAACG 
GACAGCGTCA AAGATAGCAC TTCAGAAGCG GCAGCAGTAA AGCAAGACGT CGAGGAGTTG 
GTAGCTTTAA CGCCACCTTC TCCTTTTAGA GACTCCATTG ACTCCGAAAG CACTTCCCCT 
AGTTCACCAG TTTCGGAATC GGCTCTCTGT ATTCCATCCT CTCCCAAATA TGACAATATG 
ATCATGAGAG ATTACTCCCA GAGCTCCTCC TCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947245.1
CDS	637..4155
Translation	MVLHFVLLLLLLRDDGNGNVWIMASAQTNERRVVSYMAGDIIIGALFSVHHQPTVDKVHERKCGEIREQYGIQRVEAMLHALDRINADQTLLPNISLGCEIRDSCWHTAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSAPSRSKKPIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLYKYFMRVVPSDAQQARAMVDIVKRYNWTYVSAVHTEGNYGERGMEAFKDMSAKDGICIAHSYKIYSNAGEQSFDKLLKKLRSHLPKARVVVCFCEGMTVRGLLMAMKRQNIIGEFLLLGSDGWADRYDVTDGYQREAAGGITIKLQSPDVKWFDDYYLQLRPENNLRNPWFQEFWQHRFQCKLKGYSQENQKYNRTCNSRMSLRSQYVQDSKMGFVINAIYAMAYGLHNMQTVLCPGYAGLCDAMKPIDGQKLLDSIMKANFTGVSGDMITFDENGDSPGRYEIMNFKKMGKDYYDYINVGSWDNGELKMDDDEIWSNKNLVIRSVCSDPCAKGQIKVIRKGEVSCCWTCTPCKDNEIVFDEYTCKACDLGSWPTDDLTGCDPIPVEFLRWSDPEPIAAVVFSCLGLLATMFVMAIFILYRDTPVVKSSSRELCYIILAGICLGYLCTFSLIAKPQQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFVLICIQLGIIVALFIMEPPDVIFDYPSIREVYLICNTNNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEAKYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTSTVVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLSISFTPGPTHSYCQQPIGISNGKSVTWAQNEKSSKSQHLWQRLSVHVKKKDSTNQTAVIKPFSKSSSENRYISTSFPDSSAKMLYDVSEAEEHYPVQYRPQTPSPISTVSQRAISTTRTEDDIPSFHSDTAQRSGSSQGSLMDQISSVVTRFTANISELNSMMLSANTPGTMVPTPLCSSYLIPREIQLPTTMTTFAEIQPAPNIDFNGGTQSVRKSTDSVKDSTSEAAAVKQDVEELVALTPPSPFRDSIDSESTSPSSPVSESALCIPSSPKYDNMIMRDYSQSSSSL

Target ORF information:

RefSeq Version	XM_018091756.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis glutamate receptor, metabotropic 5 (grm5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091756.1

ORF Insert Sequence:

ATGGTCCTCC ACTTTGTCCT CTTACTGCTG CTGCTAAGGG ATGATGGCAA TGGGAATGTC 
TGGATTATGG CCTCAGCACA GACCAATGAA AGGAGGGTGG TGTCCTACAT GGCCGGCGAT 
ATCATTATCG GGGCGCTGTT TTCGGTACAT CACCAACCCA CTGTGGACAA GGTCCATGAA 
AGGAAATGTG GAGAAATAAG GGAACAGTAT GGCATACAAA GAGTGGAAGC CATGTTACAT 
GCACTTGATA GAATCAACGC AGACCAAACA CTGTTGCCAA ACATATCCCT CGGATGTGAA 
ATAAGAGACT CCTGTTGGCA CACTGCGGTG GCCCTGGAGC AAAGTATTGA GTTCATCAGA 
GACTCCTTGA TTTCTTCCGA AGAGGAGGAA GGTTTGGTTC GTTGTGTTGA TGGATCTTCA 
GCGCCTTCCC GGTCAAAAAA GCCTATCGTC GGGGTTATAG GTCCTGGGTC CAGCTCTGTT 
GCTATCCAAG TGCAGAATTT ATTGCAGCTT TTCAATATAC CTCAGATTGC GTATTCGGCA 
ACAAGCATGG ACTTGAGCGA CAAAACCCTC TATAAGTATT TCATGAGAGT CGTGCCCTCA 
GATGCTCAGC AAGCCAGAGC TATGGTGGAC ATTGTGAAGA GGTACAACTG GACATATGTT 
TCGGCAGTTC ATACAGAAGG TAACTATGGG GAGAGAGGCA TGGAAGCATT CAAAGACATG 
TCGGCTAAAG ATGGCATCTG CATCGCTCAC TCCTACAAGA TTTACAGCAA TGCCGGCGAG 
CAGAGCTTCG ACAAGCTGCT AAAGAAGCTA CGGAGTCATC TCCCTAAGGC CAGGGTGGTG 
GTCTGTTTCT GCGAGGGCAT GACTGTGCGA GGGCTGCTTA TGGCCATGAA GCGCCAGAAT 
ATCATCGGAG AATTCCTTCT ACTTGGAAGT GACGGCTGGG CAGACAGGTA CGATGTGACA 
GACGGTTATC AGCGGGAAGC GGCCGGTGGA ATCACAATCA AGCTGCAGTC TCCTGATGTG 
AAATGGTTTG ATGATTATTA CCTGCAGCTA CGGCCAGAAA ACAATCTCCG AAACCCATGG 
TTCCAGGAAT TTTGGCAACA CCGGTTCCAG TGCAAACTGA AAGGGTATTC GCAGGAAAAT 
CAGAAATACA ACAGGACCTG TAATAGTCGA ATGTCTTTAC GTTCTCAGTA CGTCCAGGAT 
TCCAAAATGG GCTTCGTGAT CAATGCCATC TATGCCATGG CGTATGGGCT GCACAACATG 
CAGACAGTGC TGTGCCCGGG ATACGCCGGT CTCTGTGACG CCATGAAACC AATCGACGGT 
CAAAAACTCC TTGACTCCAT CATGAAAGCC AACTTCACGG GCGTCTCCGG CGATATGATC 
ACGTTTGATG AGAATGGAGA CTCACCAGGA AGGTATGAAA TCATGAACTT TAAGAAAATG 
GGCAAAGACT ATTACGATTA TATCAACGTT GGGAGCTGGG ATAATGGCGA ATTGAAGATG 
GATGACGATG AAATTTGGTC AAACAAAAAC TTGGTCATCC GATCAGTTTG CAGCGATCCT 
TGTGCCAAAG GACAAATAAA GGTGATCCGT AAAGGAGAGG TGAGCTGCTG CTGGACCTGC 
ACCCCCTGCA AGGATAATGA GATAGTGTTT GATGAGTACA CGTGCAAGGC CTGTGATCTG 
GGTTCTTGGC CTACAGACGA TCTGACAGGA TGTGACCCAA TCCCAGTGGA ATTTCTCCGA 
TGGAGCGACC CCGAGCCAAT CGCTGCCGTG GTTTTTTCAT GCCTTGGTCT GCTGGCCACA 
ATGTTCGTCA TGGCTATCTT TATTCTGTAC AGAGATACTC CGGTGGTGAA GTCGTCCAGC 
AGGGAGCTAT GTTATATTAT CCTAGCTGGC ATCTGCTTGG GCTACTTGTG TACCTTCTCT 
CTAATAGCCA AACCGCAACA GATTTACTGC TACCTACAAA GGATTGGCAT TGGTCTCTCC 
CCGGCTATGA GTTATTCTGC CCTTGTCACC AAAACTAACC GCATAGCCAG GATCTTGGCT 
GGGAGCAAGA AGAAAATCTG TACCAAGAAG CCTCGCTTCA TGAGCGCCTG TGCCCAGCTG 
GTCATTGCCT TCGTCCTGAT TTGTATCCAG TTAGGAATAA TTGTGGCGTT GTTCATTATG 
GAGCCGCCAG ATGTTATATT TGATTATCCC AGTATCCGGG AGGTTTACTT AATCTGCAAC 
ACGAACAACC TAGGGGTGGT CACTCCGCTT GGCTATAATG GATTATTGAT CCTGAGCTGC 
ACCTTTTACG CTTTCAAGAC TAGAAACGTT CCGGCTAATT TTAACGAAGC AAAGTACATT 
GCCTTTACCA TGTACACCAC CTGCATTATT TGGCTGGCCT TCGTGCCCAT ATATTTTGGG 
AGCAACTACA AAATTATAAC CATGTGCTTC TCGGTGAGTC TGAGCGCAAC GGTAGCCCTC 
GGCTGTATGT TTGTGCCAAA GGTGTACATT ATCCTGGCAA AGCCGGAGCG GAACGTGCGA 
AGCGCCTTCA CCACCTCCAC CGTGGTCCGT ATGCACGTAG GCGACGGGAA ATCTTCCTCA 
GCAGCTAGCC GCTCCAGTAG CTTGGTTAAT TTGTGGAAGA GAAGGGGATC GTCTGGGGAA 
ACATTAAGCA TTTCATTTAC TCCTGGGCCT ACCCACTCCT ATTGTCAGCA GCCAATCGGA 
ATCTCAAATG GCAAATCTGT TACATGGGCG CAGAATGAGA AAAGCAGTAA AAGTCAACAT 
CTCTGGCAGA GACTCTCGGT TCACGTCAAG AAGAAGGATA GCACCAATCA AACGGCGGTC 
ATTAAGCCTT TTTCAAAAAG CAGCAGTGAG AACAGATACA TCAGCACATC TTTCCCCGAC 
AGCAGTGCCA AAATGTTATA TGATGTTTCA GAGGCGGAAG AGCATTATCC AGTGCAGTAC 
CGACCGCAGA CTCCTTCGCC AATCAGTACT GTAAGCCAGA GAGCTATTTC AACAACCCGC 
ACAGAGGATG ATATTCCTTC TTTTCATTCG GATACGGCCC AAAGGAGTGG CTCCTCGCAA 
GGTTCCCTAA TGGACCAAAT CAGCAGCGTG GTGACGCGTT TCACCGCTAA CATCAGTGAG 
CTAAATTCAA TGATGCTATC TGCAAATACG CCAGGAACAA TGGTGCCCAC GCCTCTTTGT 
TCTTCCTACC TGATCCCGAG AGAGATTCAA CTGCCCACAA CAATGACCAC CTTTGCGGAG 
ATCCAACCTG CCCCTAACAT TGATTTCAAC GGCGGGACGC AGTCCGTCAG AAAATCAACG 
GACAGCGTCA AAGATAGCAC TTCAGAAGCG GCAGCAGTAA AGCAAGACGT CGAGGAGTTG 
GTAGCTTTAA CGCCACCTTC TCCTTTTAGA GACTCCATTG ACTCCGAAAG CACTTCCCCT 
AGTTCACCAG TTTCGGAATC GGCTCTCTGT ATTCCATCCT CTCCCAAATA TGACAATATG 
ATCATGAGAG ATTACTCCCA GAGCTCCTCC TCGTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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grm5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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