rnf40 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	rnf40
Entrez Gene ID	100380175
Full Name	ring finger protein 40, E3 ubiquitin protein ligase
General protein information	Preferred Names ring finger protein 40, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase BRE1B
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002940582.4	XP_002940628.1	E3 ubiquitin-protein ligase BRE1B isoform X1
XM_004918105.4	XP_004918162.1	E3 ubiquitin-protein ligase BRE1B isoform X2
XM_004918106.4	XP_004918163.1	E3 ubiquitin-protein ligase BRE1B isoform X3
XM_004918105.3	XP_004918162.1	E3 ubiquitin-protein ligase BRE1B isoform X2
XM_004918106.3	XP_004918163.1	E3 ubiquitin-protein ligase BRE1B isoform X3
XM_002940582.5	XP_002940628.1	E3 ubiquitin-protein ligase BRE1B isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rnf40	NP_758485.2
Homo sapiens (human)	RNF40	NP_055586.1
	RNF40	XP_001102375.2
Pan troglodytes (chimpanzee)	RNF40	XP_003315121.1
Rattus norvegicus (Norway rat)	Rnf40	NP_703201.1
Xenopus tropicalis (tropical clawed frog)	rnf40	XP_004918162.1
Drosophila melanogaster (fruit fly)	Bre1	NP_647989.2
Canis lupus familiaris (dog)	RNF40	XP_005621328.1
Bos taurus (cattle)	RNF40	NP_001193381.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007335	XP_308493.4
Caenorhabditis elegans (roundworm)	rfp-1	NP_001022700.1

Gene Ontology

The following rnf40 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rnf40 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa16540	XM_002940582.5 Latest version!	Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa16541	XM_004918105.4 Latest version!	Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa16542	XM_004918106.4 Latest version!	Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa16541	XM_004918105.3	Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa16542	XM_004918106.3	Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa16540	XM_002940582.4	Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rnf40 ( XM_002940582.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940628.1 Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa16540
Clone ID Related Accession (Same CDS sequence)	XM_002940582.4 , XM_002940582.5
Accession Version	XM_002940582.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase BRE1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940582.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGGG CCGGAAGTAA GCGAGCCTCG AGCGACAATG GCTCTGGCCC TTCAGAAAAG 
AAACCAAATC TTGAAGCCAA GACGCCCACT ACTTTAATTG AGCCCATACG ACTAGGGGGC 
ATCTCCTCAA CAGAGGAGAT GGATATGAAA GTCCTGCAGT TCAAGAACAA GAAACTGGCG 
GAACGCCTGG AGCAGAGGCA GGGCTTTGAA GATGAACTCC GGGAGAAGAT CGAGAAGCTG 
GAGAAAAGAC AGGCTACTGA CGACGCTACT TTGCTCATTG TGAACCGTTA TTGGAGCAAG 
CTGGATGAGG ATGTTCACGT GCTGCTAAAG CGTTATGATT CCGAGCCAAG TAAGCAGAGT 
GCTGCGCCTA GCGCTGCTAC CGAGGAACCA GAGAGTGCCA AGGAGACTGC CATGGAAACA 
GACGAGAGTG CAGAGGGCTC ACAGCCCCCA CCAACCCAAG AAATCAAAGC AGCTTCCCCG 
GAACCTCCCC CCGCTCCTCC TTTCTCTACC GGAGCGTTGC CAGAATCTTC TTTGACCTTC 
TTAGCCACAT TAGCCCACTG TACCAAAGAG GAGATTGAGC TGAAGCTTCA AGAACGCATG 
GAGTTTAGCA AGAAGGCTGT ATCCTGTGTT CTGGAAACTT CTAACAAGCT CCACTGCCGA 
ACGGAAGAGC TATGTGACCG AATCCACAAA GGCGATTTCG ATCACTTGGG GGAATCGCCT 
AAAAAACTGA ACAAAGAACT TATCACTGAG AACCGTAGGC TGCAGGACCT CGCTACTGTG 
CTACAGGAGA AGCATCACCG ACTCTCCCTG CAGCATCATG AGCTCCAGGA CCGTGCAACT 
TCAACAGAGA CCAAGGTGTC CGAGATGGAG AGGACCATTG AGGACCTGCA GTGGGACATT 
GAGAAATTAC GCAAGCATGA ACAGAAGCTA AACCGTCATC TGGCAGAGGC CCTGGAGCAG 
CTAAATTCTG GATACCACAT CTCTGGAAGT TCTGGAAGTT TCCAGGGAGG ACAGACTACC 
CTAAGTATGC AAAAGTTTGA AATGTTGAAT GCAGAACTCG ATCAGAACCA GGAACTTGCA 
AACAGTCGGA TTTCCGAGCT GGAAAAACTG CAGAATGAAC TACAGGCAGC TGTCAGGCTA 
AAGGAGAAGC TAAAGCTGGA CCTGCGGACA TTGCCTGAGG AAGCGGTACG AAACACGCAG 
GATTTCCGCT GCTTGCAGTC TCAATTCTCT CTCCTGTACA ATGAGTCTCT GCAAGTAAAG 
ACCCAGTTGG ATGAGGCCCG AGCGCTGCTG TTAAGCACCA AGAACAGCCA CCTGAGACAG 
ATTGAACGCA TGGAGAGCGA GGAGCTGTCT GTACAAAAGA AACTGCGCAC TGAAGTGATC 
CAGTTGGAGG ATACGCTCGC CCAGGTGCGC AAAGAATATG AAATGCTGAG GATAGAGTTT 
GAGCAGAACC TGGCAGCCAA TGAGCAAGCA GGTCCTATTA ATAGGGAGAT GCGGCATCTC 
ATTGGAAGCC TCCAAAACCA CAACCACCAA CTGAAGGGAG ATGTGCAAAG ATACAAGCGC 
AAGGTGCGAG ATTCCCAGGC AGAGGTGAGC AAGCTGCGTT TTCAAATCGG CGGACCACCC 
TGTCCTCAGC CTGGATCTTC AGGATCGGAA CCAACGACCC CCGTTACTGT CAAAGAGGAA 
GAAGTAGAAG TGAAGAAAGA ACCCCTTGCA GTCGTGCCAC GAGAGCCTGA GACTCCCACT 
ACCCCTGAAG TAAAAAAGGA GGAGGAAGAG CAAAAGAAAG AGGCAGCAGA GCGGACCAAA 
GCCCGGGAGA GGGAGGCGGA ACGGGAGCGA GACAAAGATC GGGAAAGAGA CAAGGAAAAG 
GAAAGGGAGC GTGAGAAAGA CAAGCAGAAA ACAGATGAGA GCAAAAGGAA AGACTGCGAC 
ATTCTGAAAC AGCTCCGGGC AGAGCTCAAG AAGGCCCAGG AGAGCCAGAA AGAGATGAAG 
CTGCTGCTCG ATATGTACAA ATCTGCCCCC AAAGAGCAGA GAGACAAGGT GCAGCTGATG 
GCAGCGGAGA GAAAGACCAA GGCCGAGATG GAGGAGCAGC GCACGCGGGT GCAAGCTCTT 
GAGGAGAAAG ACAAGAGGGA GAGGAAGAAA CTTGCAGATG AAGAAGCACT GAGGAAAATT 
AAGATGGCTG AGGAACAAAT AGACCATTTA CAGAGGAAAC TGACTGCCAC CAAACAGGAG 
GAGGAGGCCT TACTGTCAGA GATGGACGTG ACGGGCCAGG CGTTTGAGGA CATGCAAGAA 
CAGAATATTC GTCTGATGCA GCAGCTCCGT GAGAAGGACG ACGCCAACTT CAAACTGATG 
TCCGAGAGGA TCAAATCCAA CCAGATACAC AAACTCCTGA GGGAAGAGAA GGAGGAGCTG 
GCTGAGCAAG TGCAAGGGCT GAAAGCCCAG GTGGATGCTC AGCTGTTACA GGTACAGAAA 
CTGGAGGAGA GAGAGCGCCT CTTACACACG TCTTTGACTT CCATTGAGAA GGAAGTCACT 
CTTAGAACAC AGTCACTGGA ACTGAACAAG AGGAAGGCAG TCGAGGCAGC CCAGCTCGCC 
GAAGACCTGA AAGTGCAAAT GGAGCATGTA GGGGAAACGC TTAGGGATAC CCAGAACTTT 
GTATGTGAGA ACCGAAGCGC TAAAGAAAAG GAGTCCTTTA GTCTCAAAAG AGCTCAGGAG 
GATGTATCTC GGCTGCGAAG GAAACTGGAA AAGCAGAAGA AGATGGAGGT TTATGCAGAT 
GCTGACCAAA TCCTACAGGA AGAGATTAAA GAGTACAGGG CCCGACTCAC TTGCCCTTGC 
TGTAACACTC GGAAAAAGGA CGCCGTCCTC ACCAAGTGCT TTCACGTCTT CTGCTTCGAG 
TGCGTGAAGA CCCGATACGA GTCCCGGCAG CGCAAGTGCC CCAAGTGCAA CGCAGCCTTC 
GGAGCCCACG ACTTTCACCG GATTTACATC AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940628.1
CDS	172..3147
Translation	MSGAGSKRASSDNGSGPSEKKPNLEAKTPTTLIEPIRLGGISSTEEMDMKVLQFKNKKLAERLEQRQGFEDELREKIEKLEKRQATDDATLLIVNRYWSKLDEDVHVLLKRYDSEPSKQSAAPSAATEEPESAKETAMETDESAEGSQPPPTQEIKAASPEPPPAPPFSTGALPESSLTFLATLAHCTKEEIELKLQERMEFSKKAVSCVLETSNKLHCRTEELCDRIHKGDFDHLGESPKKLNKELITENRRLQDLATVLQEKHHRLSLQHHELQDRATSTETKVSEMERTIEDLQWDIEKLRKHEQKLNRHLAEALEQLNSGYHISGSSGSFQGGQTTLSMQKFEMLNAELDQNQELANSRISELEKLQNELQAAVRLKEKLKLDLRTLPEEAVRNTQDFRCLQSQFSLLYNESLQVKTQLDEARALLLSTKNSHLRQIERMESEELSVQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLIGSLQNHNHQLKGDVQRYKRKVRDSQAEVSKLRFQIGGPPCPQPGSSGSEPTTPVTVKEEEVEVKKEPLAVVPREPETPTTPEVKKEEEEQKKEAAERTKAREREAERERDKDRERDKEKEREREKDKQKTDESKRKDCDILKQLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKTKAEMEEQRTRVQALEEKDKRERKKLADEEALRKIKMAEEQIDHLQRKLTATKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLREEKEELAEQVQGLKAQVDAQLLQVQKLEERERLLHTSLTSIEKEVTLRTQSLELNKRKAVEAAQLAEDLKVQMEHVGETLRDTQNFVCENRSAKEKESFSLKRAQEDVSRLRRKLEKQKKMEVYADADQILQEEIKEYRARLTCPCCNTRKKDAVLTKCFHVFCFECVKTRYESRQRKCPKCNAAFGAHDFHRIYIN

Target ORF information:

RefSeq Version	XM_002940582.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940582.4

ORF Insert Sequence:

ATGTCAGGGG CCGGAAGTAA GCGAGCCTCG AGCGACAATG GCTCTGGCCC TTCAGAAAAG 
AAACCAAATC TTGAAGCCAA GACGCCCACT ACTTTAATTG AGCCCATACG ACTAGGGGGC 
ATCTCCTCAA CAGAGGAGAT GGATATGAAA GTCCTGCAGT TCAAGAACAA GAAACTGGCG 
GAACGCCTGG AGCAGAGGCA GGGCTTTGAA GATGAACTCC GGGAGAAGAT CGAGAAGCTG 
GAGAAAAGAC AGGCTACTGA CGACGCTACT TTGCTCATTG TGAACCGTTA TTGGAGCAAG 
CTGGATGAGG ATGTTCACGT GCTGCTAAAG CGTTATGATT CCGAGCCAAG TAAGCAGAGT 
GCTGCGCCTA GCGCTGCTAC CGAGGAACCA GAGAGTGCCA AGGAGACTGC CATGGAAACA 
GACGAGAGTG CAGAGGGCTC ACAGCCCCCA CCAACCCAAG AAATCAAAGC AGCTTCCCCG 
GAACCTCCCC CCGCTCCTCC TTTCTCTACC GGAGCGTTGC CAGAATCTTC TTTGACCTTC 
TTAGCCACAT TAGCCCACTG TACCAAAGAG GAGATTGAGC TGAAGCTTCA AGAACGCATG 
GAGTTTAGCA AGAAGGCTGT ATCCTGTGTT CTGGAAACTT CTAACAAGCT CCACTGCCGA 
ACGGAAGAGC TATGTGACCG AATCCACAAA GGCGATTTCG ATCACTTGGG GGAATCGCCT 
AAAAAACTGA ACAAAGAACT TATCACTGAG AACCGTAGGC TGCAGGACCT CGCTACTGTG 
CTACAGGAGA AGCATCACCG ACTCTCCCTG CAGCATCATG AGCTCCAGGA CCGTGCAACT 
TCAACAGAGA CCAAGGTGTC CGAGATGGAG AGGACCATTG AGGACCTGCA GTGGGACATT 
GAGAAATTAC GCAAGCATGA ACAGAAGCTA AACCGTCATC TGGCAGAGGC CCTGGAGCAG 
CTAAATTCTG GATACCACAT CTCTGGAAGT TCTGGAAGTT TCCAGGGAGG ACAGACTACC 
CTAAGTATGC AAAAGTTTGA AATGTTGAAT GCAGAACTCG ATCAGAACCA GGAACTTGCA 
AACAGTCGGA TTTCCGAGCT GGAAAAACTG CAGAATGAAC TACAGGCAGC TGTCAGGCTA 
AAGGAGAAGC TAAAGCTGGA CCTGCGGACA TTGCCTGAGG AAGCGGTACG AAACACGCAG 
GATTTCCGCT GCTTGCAGTC TCAATTCTCT CTCCTGTACA ATGAGTCTCT GCAAGTAAAG 
ACCCAGTTGG ATGAGGCCCG AGCGCTGCTG TTAAGCACCA AGAACAGCCA CCTGAGACAG 
ATTGAACGCA TGGAGAGCGA GGAGCTGTCT GTACAAAAGA AACTGCGCAC TGAAGTGATC 
CAGTTGGAGG ATACGCTCGC CCAGGTGCGC AAAGAATATG AAATGCTGAG GATAGAGTTT 
GAGCAGAACC TGGCAGCCAA TGAGCAAGCA GGTCCTATTA ATAGGGAGAT GCGGCATCTC 
ATTGGAAGCC TCCAAAACCA CAACCACCAA CTGAAGGGAG ATGTGCAAAG ATACAAGCGC 
AAGGTGCGAG ATTCCCAGGC AGAGGTGAGC AAGCTGCGTT TTCAAATCGG CGGACCACCC 
TGTCCTCAGC CTGGATCTTC AGGATCGGAA CCAACGACCC CCGTTACTGT CAAAGAGGAA 
GAAGTAGAAG TGAAGAAAGA ACCCCTTGCA GTCGTGCCAC GAGAGCCTGA GACTCCCACT 
ACCCCTGAAG TAAAAAAGGA GGAGGAAGAG CAAAAGAAAG AGGCAGCAGA GCGGACCAAA 
GCCCGGGAGA GGGAGGCGGA ACGGGAGCGA GACAAAGATC GGGAAAGAGA CAAGGAAAAG 
GAAAGGGAGC GTGAGAAAGA CAAGCAGAAA ACAGATGAGA GCAAAAGGAA AGACTGCGAC 
ATTCTGAAAC AGCTCCGGGC AGAGCTCAAG AAGGCCCAGG AGAGCCAGAA AGAGATGAAG 
CTGCTGCTCG ATATGTACAA ATCTGCCCCC AAAGAGCAGA GAGACAAGGT GCAGCTGATG 
GCAGCGGAGA GAAAGACCAA GGCCGAGATG GAGGAGCAGC GCACGCGGGT GCAAGCTCTT 
GAGGAGAAAG ACAAGAGGGA GAGGAAGAAA CTTGCAGATG AAGAAGCACT GAGGAAAATT 
AAGATGGCTG AGGAACAAAT AGACCATTTA CAGAGGAAAC TGACTGCCAC CAAACAGGAG 
GAGGAGGCCT TACTGTCAGA GATGGACGTG ACGGGCCAGG CGTTTGAGGA CATGCAAGAA 
CAGAATATTC GTCTGATGCA GCAGCTCCGT GAGAAGGACG ACGCCAACTT CAAACTGATG 
TCCGAGAGGA TCAAATCCAA CCAGATACAC AAACTCCTGA GGGAAGAGAA GGAGGAGCTG 
GCTGAGCAAG TGCAAGGGCT GAAAGCCCAG GTGGATGCTC AGCTGTTACA GGTACAGAAA 
CTGGAGGAGA GAGAGCGCCT CTTACACACG TCTTTGACTT CCATTGAGAA GGAAGTCACT 
CTTAGAACAC AGTCACTGGA ACTGAACAAG AGGAAGGCAG TCGAGGCAGC CCAGCTCGCC 
GAAGACCTGA AAGTGCAAAT GGAGCATGTA GGGGAAACGC TTAGGGATAC CCAGAACTTT 
GTATGTGAGA ACCGAAGCGC TAAAGAAAAG GAGTCCTTTA GTCTCAAAAG AGCTCAGGAG 
GATGTATCTC GGCTGCGAAG GAAACTGGAA AAGCAGAAGA AGATGGAGGT TTATGCAGAT 
GCTGACCAAA TCCTACAGGA AGAGATTAAA GAGTACAGGG CCCGACTCAC TTGCCCTTGC 
TGTAACACTC GGAAAAAGGA CGCCGTCCTC ACCAAGTGCT TTCACGTCTT CTGCTTCGAG 
TGCGTGAAGA CCCGATACGA GTCCCGGCAG CGCAAGTGCC CCAAGTGCAA CGCAGCCTTC 
GGAGCCCACG ACTTTCACCG GATTTACATC AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rnf40 ( XM_004918105.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918162.1 Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16541
Clone ID Related Accession (Same CDS sequence)	XM_004918105.4 , XM_004918105.3
Accession Version	XM_004918105.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase BRE1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004918105.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGGG CCGGAAGTAA GCGAGCCTCG AGCGACAATG GCTCTGGCCC TTCAGAAAAG 
AAACCAAATC TTGAAGCCAA GACGCCCACT ACTTTAATTG AGCCCATACG ACTAGGGGGC 
ATCTCCTCAA CAGAGGAGAT GGATATGAAA GTCCTGCAGT TCAAGAACAA GAAACTGGCG 
GAACGCCTGG AGCAGAGGCA GGGCTTTGAA GATGAACTCC GGGAGAAGAT CGAGAAGCTG 
GAGAAAAGAC AGGCTACTGA CGACGCTACT TTGCTCATTG TGAACCGTTA TTGGAGCAAG 
CTGGATGAGG ATGTTCACGT GCTGCTAAAG CGTTATGATT CCGAGCCAAG TAAGCAGAGT 
GCTGCGCCTA GCGCTGCTAC CGAGGAACCA GAGAGTGCCA AGGAGACTGC CATGGAAACA 
GACGAGAGTG CAGAGGGCTC ACAGCCCCCA CCAACCCAAG AAATCAAAGC TTCCCCGGAA 
CCTCCCCCCG CTCCTCCTTT CTCTACCGGA GCGTTGCCAG AATCTTCTTT GACCTTCTTA 
GCCACATTAG CCCACTGTAC CAAAGAGGAG ATTGAGCTGA AGCTTCAAGA ACGCATGGAG 
TTTAGCAAGA AGGCTGTATC CTGTGTTCTG GAAACTTCTA ACAAGCTCCA CTGCCGAACG 
GAAGAGCTAT GTGACCGAAT CCACAAAGGC GATTTCGATC ACTTGGGGGA ATCGCCTAAA 
AAACTGAACA AAGAACTTAT CACTGAGAAC CGTAGGCTGC AGGACCTCGC TACTGTGCTA 
CAGGAGAAGC ATCACCGACT CTCCCTGCAG CATCATGAGC TCCAGGACCG TGCAACTTCA 
ACAGAGACCA AGGTGTCCGA GATGGAGAGG ACCATTGAGG ACCTGCAGTG GGACATTGAG 
AAATTACGCA AGCATGAACA GAAGCTAAAC CGTCATCTGG CAGAGGCCCT GGAGCAGCTA 
AATTCTGGAT ACCACATCTC TGGAAGTTCT GGAAGTTTCC AGGGAGGACA GACTACCCTA 
AGTATGCAAA AGTTTGAAAT GTTGAATGCA GAACTCGATC AGAACCAGGA ACTTGCAAAC 
AGTCGGATTT CCGAGCTGGA AAAACTGCAG AATGAACTAC AGGCAGCTGT CAGGCTAAAG 
GAGAAGCTAA AGCTGGACCT GCGGACATTG CCTGAGGAAG CGGTACGAAA CACGCAGGAT 
TTCCGCTGCT TGCAGTCTCA ATTCTCTCTC CTGTACAATG AGTCTCTGCA AGTAAAGACC 
CAGTTGGATG AGGCCCGAGC GCTGCTGTTA AGCACCAAGA ACAGCCACCT GAGACAGATT 
GAACGCATGG AGAGCGAGGA GCTGTCTGTA CAAAAGAAAC TGCGCACTGA AGTGATCCAG 
TTGGAGGATA CGCTCGCCCA GGTGCGCAAA GAATATGAAA TGCTGAGGAT AGAGTTTGAG 
CAGAACCTGG CAGCCAATGA GCAAGCAGGT CCTATTAATA GGGAGATGCG GCATCTCATT 
GGAAGCCTCC AAAACCACAA CCACCAACTG AAGGGAGATG TGCAAAGATA CAAGCGCAAG 
GTGCGAGATT CCCAGGCAGA GGTGAGCAAG CTGCGTTTTC AAATCGGCGG ACCACCCTGT 
CCTCAGCCTG GATCTTCAGG ATCGGAACCA ACGACCCCCG TTACTGTCAA AGAGGAAGAA 
GTAGAAGTGA AGAAAGAACC CCTTGCAGTC GTGCCACGAG AGCCTGAGAC TCCCACTACC 
CCTGAAGTAA AAAAGGAGGA GGAAGAGCAA AAGAAAGAGG CAGCAGAGCG GACCAAAGCC 
CGGGAGAGGG AGGCGGAACG GGAGCGAGAC AAAGATCGGG AAAGAGACAA GGAAAAGGAA 
AGGGAGCGTG AGAAAGACAA GCAGAAAACA GATGAGAGCA AAAGGAAAGA CTGCGACATT 
CTGAAACAGC TCCGGGCAGA GCTCAAGAAG GCCCAGGAGA GCCAGAAAGA GATGAAGCTG 
CTGCTCGATA TGTACAAATC TGCCCCCAAA GAGCAGAGAG ACAAGGTGCA GCTGATGGCA 
GCGGAGAGAA AGACCAAGGC CGAGATGGAG GAGCAGCGCA CGCGGGTGCA AGCTCTTGAG 
GAGAAAGACA AGAGGGAGAG GAAGAAACTT GCAGATGAAG AAGCACTGAG GAAAATTAAG 
ATGGCTGAGG AACAAATAGA CCATTTACAG AGGAAACTGA CTGCCACCAA ACAGGAGGAG 
GAGGCCTTAC TGTCAGAGAT GGACGTGACG GGCCAGGCGT TTGAGGACAT GCAAGAACAG 
AATATTCGTC TGATGCAGCA GCTCCGTGAG AAGGACGACG CCAACTTCAA ACTGATGTCC 
GAGAGGATCA AATCCAACCA GATACACAAA CTCCTGAGGG AAGAGAAGGA GGAGCTGGCT 
GAGCAAGTGC AAGGGCTGAA AGCCCAGGTG GATGCTCAGC TGTTACAGGT ACAGAAACTG 
GAGGAGAGAG AGCGCCTCTT ACACACGTCT TTGACTTCCA TTGAGAAGGA AGTCACTCTT 
AGAACACAGT CACTGGAACT GAACAAGAGG AAGGCAGTCG AGGCAGCCCA GCTCGCCGAA 
GACCTGAAAG TGCAAATGGA GCATGTAGGG GAAACGCTTA GGGATACCCA GAACTTTGTA 
TGTGAGAACC GAAGCGCTAA AGAAAAGGAG TCCTTTAGTC TCAAAAGAGC TCAGGAGGAT 
GTATCTCGGC TGCGAAGGAA ACTGGAAAAG CAGAAGAAGA TGGAGGTTTA TGCAGATGCT 
GACCAAATCC TACAGGAAGA GATTAAAGAG TACAGGGCCC GACTCACTTG CCCTTGCTGT 
AACACTCGGA AAAAGGACGC CGTCCTCACC AAGTGCTTTC ACGTCTTCTG CTTCGAGTGC 
GTGAAGACCC GATACGAGTC CCGGCAGCGC AAGTGCCCCA AGTGCAACGC AGCCTTCGGA 
GCCCACGACT TTCACCGGAT TTACATCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918162.1
CDS	172..3144
Translation	MSGAGSKRASSDNGSGPSEKKPNLEAKTPTTLIEPIRLGGISSTEEMDMKVLQFKNKKLAERLEQRQGFEDELREKIEKLEKRQATDDATLLIVNRYWSKLDEDVHVLLKRYDSEPSKQSAAPSAATEEPESAKETAMETDESAEGSQPPPTQEIKASPEPPPAPPFSTGALPESSLTFLATLAHCTKEEIELKLQERMEFSKKAVSCVLETSNKLHCRTEELCDRIHKGDFDHLGESPKKLNKELITENRRLQDLATVLQEKHHRLSLQHHELQDRATSTETKVSEMERTIEDLQWDIEKLRKHEQKLNRHLAEALEQLNSGYHISGSSGSFQGGQTTLSMQKFEMLNAELDQNQELANSRISELEKLQNELQAAVRLKEKLKLDLRTLPEEAVRNTQDFRCLQSQFSLLYNESLQVKTQLDEARALLLSTKNSHLRQIERMESEELSVQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLIGSLQNHNHQLKGDVQRYKRKVRDSQAEVSKLRFQIGGPPCPQPGSSGSEPTTPVTVKEEEVEVKKEPLAVVPREPETPTTPEVKKEEEEQKKEAAERTKAREREAERERDKDRERDKEKEREREKDKQKTDESKRKDCDILKQLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKTKAEMEEQRTRVQALEEKDKRERKKLADEEALRKIKMAEEQIDHLQRKLTATKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLREEKEELAEQVQGLKAQVDAQLLQVQKLEERERLLHTSLTSIEKEVTLRTQSLELNKRKAVEAAQLAEDLKVQMEHVGETLRDTQNFVCENRSAKEKESFSLKRAQEDVSRLRRKLEKQKKMEVYADADQILQEEIKEYRARLTCPCCNTRKKDAVLTKCFHVFCFECVKTRYESRQRKCPKCNAAFGAHDFHRIYIN

Target ORF information:

RefSeq Version	XM_004918105.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918105.3

ORF Insert Sequence:

ATGTCAGGGG CCGGAAGTAA GCGAGCCTCG AGCGACAATG GCTCTGGCCC TTCAGAAAAG 
AAACCAAATC TTGAAGCCAA GACGCCCACT ACTTTAATTG AGCCCATACG ACTAGGGGGC 
ATCTCCTCAA CAGAGGAGAT GGATATGAAA GTCCTGCAGT TCAAGAACAA GAAACTGGCG 
GAACGCCTGG AGCAGAGGCA GGGCTTTGAA GATGAACTCC GGGAGAAGAT CGAGAAGCTG 
GAGAAAAGAC AGGCTACTGA CGACGCTACT TTGCTCATTG TGAACCGTTA TTGGAGCAAG 
CTGGATGAGG ATGTTCACGT GCTGCTAAAG CGTTATGATT CCGAGCCAAG TAAGCAGAGT 
GCTGCGCCTA GCGCTGCTAC CGAGGAACCA GAGAGTGCCA AGGAGACTGC CATGGAAACA 
GACGAGAGTG CAGAGGGCTC ACAGCCCCCA CCAACCCAAG AAATCAAAGC TTCCCCGGAA 
CCTCCCCCCG CTCCTCCTTT CTCTACCGGA GCGTTGCCAG AATCTTCTTT GACCTTCTTA 
GCCACATTAG CCCACTGTAC CAAAGAGGAG ATTGAGCTGA AGCTTCAAGA ACGCATGGAG 
TTTAGCAAGA AGGCTGTATC CTGTGTTCTG GAAACTTCTA ACAAGCTCCA CTGCCGAACG 
GAAGAGCTAT GTGACCGAAT CCACAAAGGC GATTTCGATC ACTTGGGGGA ATCGCCTAAA 
AAACTGAACA AAGAACTTAT CACTGAGAAC CGTAGGCTGC AGGACCTCGC TACTGTGCTA 
CAGGAGAAGC ATCACCGACT CTCCCTGCAG CATCATGAGC TCCAGGACCG TGCAACTTCA 
ACAGAGACCA AGGTGTCCGA GATGGAGAGG ACCATTGAGG ACCTGCAGTG GGACATTGAG 
AAATTACGCA AGCATGAACA GAAGCTAAAC CGTCATCTGG CAGAGGCCCT GGAGCAGCTA 
AATTCTGGAT ACCACATCTC TGGAAGTTCT GGAAGTTTCC AGGGAGGACA GACTACCCTA 
AGTATGCAAA AGTTTGAAAT GTTGAATGCA GAACTCGATC AGAACCAGGA ACTTGCAAAC 
AGTCGGATTT CCGAGCTGGA AAAACTGCAG AATGAACTAC AGGCAGCTGT CAGGCTAAAG 
GAGAAGCTAA AGCTGGACCT GCGGACATTG CCTGAGGAAG CGGTACGAAA CACGCAGGAT 
TTCCGCTGCT TGCAGTCTCA ATTCTCTCTC CTGTACAATG AGTCTCTGCA AGTAAAGACC 
CAGTTGGATG AGGCCCGAGC GCTGCTGTTA AGCACCAAGA ACAGCCACCT GAGACAGATT 
GAACGCATGG AGAGCGAGGA GCTGTCTGTA CAAAAGAAAC TGCGCACTGA AGTGATCCAG 
TTGGAGGATA CGCTCGCCCA GGTGCGCAAA GAATATGAAA TGCTGAGGAT AGAGTTTGAG 
CAGAACCTGG CAGCCAATGA GCAAGCAGGT CCTATTAATA GGGAGATGCG GCATCTCATT 
GGAAGCCTCC AAAACCACAA CCACCAACTG AAGGGAGATG TGCAAAGATA CAAGCGCAAG 
GTGCGAGATT CCCAGGCAGA GGTGAGCAAG CTGCGTTTTC AAATCGGCGG ACCACCCTGT 
CCTCAGCCTG GATCTTCAGG ATCGGAACCA ACGACCCCCG TTACTGTCAA AGAGGAAGAA 
GTAGAAGTGA AGAAAGAACC CCTTGCAGTC GTGCCACGAG AGCCTGAGAC TCCCACTACC 
CCTGAAGTAA AAAAGGAGGA GGAAGAGCAA AAGAAAGAGG CAGCAGAGCG GACCAAAGCC 
CGGGAGAGGG AGGCGGAACG GGAGCGAGAC AAAGATCGGG AAAGAGACAA GGAAAAGGAA 
AGGGAGCGTG AGAAAGACAA GCAGAAAACA GATGAGAGCA AAAGGAAAGA CTGCGACATT 
CTGAAACAGC TCCGGGCAGA GCTCAAGAAG GCCCAGGAGA GCCAGAAAGA GATGAAGCTG 
CTGCTCGATA TGTACAAATC TGCCCCCAAA GAGCAGAGAG ACAAGGTGCA GCTGATGGCA 
GCGGAGAGAA AGACCAAGGC CGAGATGGAG GAGCAGCGCA CGCGGGTGCA AGCTCTTGAG 
GAGAAAGACA AGAGGGAGAG GAAGAAACTT GCAGATGAAG AAGCACTGAG GAAAATTAAG 
ATGGCTGAGG AACAAATAGA CCATTTACAG AGGAAACTGA CTGCCACCAA ACAGGAGGAG 
GAGGCCTTAC TGTCAGAGAT GGACGTGACG GGCCAGGCGT TTGAGGACAT GCAAGAACAG 
AATATTCGTC TGATGCAGCA GCTCCGTGAG AAGGACGACG CCAACTTCAA ACTGATGTCC 
GAGAGGATCA AATCCAACCA GATACACAAA CTCCTGAGGG AAGAGAAGGA GGAGCTGGCT 
GAGCAAGTGC AAGGGCTGAA AGCCCAGGTG GATGCTCAGC TGTTACAGGT ACAGAAACTG 
GAGGAGAGAG AGCGCCTCTT ACACACGTCT TTGACTTCCA TTGAGAAGGA AGTCACTCTT 
AGAACACAGT CACTGGAACT GAACAAGAGG AAGGCAGTCG AGGCAGCCCA GCTCGCCGAA 
GACCTGAAAG TGCAAATGGA GCATGTAGGG GAAACGCTTA GGGATACCCA GAACTTTGTA 
TGTGAGAACC GAAGCGCTAA AGAAAAGGAG TCCTTTAGTC TCAAAAGAGC TCAGGAGGAT 
GTATCTCGGC TGCGAAGGAA ACTGGAAAAG CAGAAGAAGA TGGAGGTTTA TGCAGATGCT 
GACCAAATCC TACAGGAAGA GATTAAAGAG TACAGGGCCC GACTCACTTG CCCTTGCTGT 
AACACTCGGA AAAAGGACGC CGTCCTCACC AAGTGCTTTC ACGTCTTCTG CTTCGAGTGC 
GTGAAGACCC GATACGAGTC CCGGCAGCGC AAGTGCCCCA AGTGCAACGC AGCCTTCGGA 
GCCCACGACT TTCACCGGAT TTACATCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rnf40 ( XM_004918106.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918163.1 Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa16542
Clone ID Related Accession (Same CDS sequence)	XM_004918106.4 , XM_004918106.3
Accession Version	XM_004918106.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase BRE1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004918106.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGGG CCGGAAGTAA GCGAGCCTCG AGCGACAATG GCTCTGGCCC TTCAGAAAAG 
AAACCAAATC TTGAAGCCAA GACGCCCACT ACTTTAATTG AGCCCATACG ACTAGGGGGC 
ATCTCCTCAA CAGAGGAGAT GGATATGAAA GTCCTGCAGT TCAAGAACAA GAAACTGGCG 
GAACGCCTGG AGCAGAGGCA GGGCTTTGAA GATGAACTCC GGGAGAAGAT CGAGAAGCTG 
GAGAAAAGAC AGGCTACTGA CGACGCTACT TTGCTCATTG TGAACCGTTA TTGGAGCAAG 
CTGGATGAGG ATGTTCACGT GCTGCTAAAG CGTTATGATT CCGAGCCAAG TAAGCAGAGT 
GCTGCGCCTA GCGCTGCTAC CGAGGAACCA GAGAGTGCCA AGGAGACTGC CATGGAAACA 
GACGAGAGTG CAGAGGGCTC ACAGCCCCCA CCAACCCAAG AAATCAAAGC AGCTTCCCCG 
GAACCTCCCC CCGCTCCTCC TTTCTCTACC GGAGCGTTGC CAGAATCTTC TTTGACCTTC 
TTAGCCACAT TAGCCCACTG TACCAAAGAG GAGATTGAGC TGAAGCTTCA AGAACGCATG 
GAGTTTAGCA AGAAGGCTGT ATCCTGTGTT CTGGAAACTT CTAACAAGCT CCACTGCCGA 
ACGGAAGAGC TATGTGACCG AATCCACAAA GGCGATTTCG ATCACTTGGG GGAATCGCCT 
AAAAAACTGA ACAAAGAACT TATCACTGAG AACCGTAGGC TGCAGGACCT CGCTACTGTG 
CTACAGGAGA AGCATCACCG ACTCTCCCTG CAGCATCATG AGCTCCAGGA CCGTGCAACT 
TCAACAGAGA CCAAGGTGTC CGAGATGGAG AGGACCATTG AGGACCTGCA GTGGGACATT 
GAGAAATTAC GCAAGCATGA ACAGAAGCTA AACCGTCATC TGGCAGAGGC CCTGGAGCAG 
CTAAATTCTG GATACCACAT CTCTGGAAGT TCTGGAAGTT TCCAGGGAGG ACAGACTACC 
CTAAGTATGC AAAAGTTTGA AATGTTGAAT GCAGAACTCG ATCAGAACCA GGAACTTGCA 
AACAGTCGGA TTTCCGAGCT GGAAAAACTG CAGAATGAAC TACAGGCAGC TGTCAGGCTA 
AAGGAGAAGC TAAAGCTGGA CCTGCGGACA TTGCCTGAGG AAGCGGTACG AAACACGCAG 
GATTTCCGCT GCTTGCAGTC TCAATTCTCT CTCCTGTACA ATGAGTCTCT GCAAGTAAAG 
ACCCAGTTGG ATGAGGCCCG AGCGCTGCTG TTAAGCACCA AGAACAGCCA CCTGAGACAG 
ATTGAACGCA TGGAGAGCGA GGAGCTGTCT GTACAAAAGA AACTGCGCAC TGAAGTGATC 
CAGTTGGAGG ATACGCTCGC CCAGGTGCGC AAAGAATATG AAATGCTGAG GATAGAGTTT 
GAGCAGAACC TGGCAGCCAA TGAGCAAGCA GGTCCTATTA ATAGGGAGAT GCGGCATCTC 
ATTGGAAGCC TCCAAAACCA CAACCACCAA CTGAAGGGAG ATGTGCAAAG ATACAAGCGC 
AAGGTGCGAG ATTCCCAGGC AGAGCTGCGT TTTCAAATCG GCGGACCACC CTGTCCTCAG 
CCTGGATCTT CAGGATCGGA ACCAACGACC CCCGTTACTG TCAAAGAGGA AGAAGTAGAA 
GTGAAGAAAG AACCCCTTGC AGTCGTGCCA CGAGAGCCTG AGACTCCCAC TACCCCTGAA 
GTAAAAAAGG AGGAGGAAGA GCAAAAGAAA GAGGCAGCAG AGCGGACCAA AGCCCGGGAG 
AGGGAGGCGG AACGGGAGCG AGACAAAGAT CGGGAAAGAG ACAAGGAAAA GGAAAGGGAG 
CGTGAGAAAG ACAAGCAGAA AACAGATGAG AGCAAAAGGA AAGACTGCGA CATTCTGAAA 
CAGCTCCGGG CAGAGCTCAA GAAGGCCCAG GAGAGCCAGA AAGAGATGAA GCTGCTGCTC 
GATATGTACA AATCTGCCCC CAAAGAGCAG AGAGACAAGG TGCAGCTGAT GGCAGCGGAG 
AGAAAGACCA AGGCCGAGAT GGAGGAGCAG CGCACGCGGG TGCAAGCTCT TGAGGAGAAA 
GACAAGAGGG AGAGGAAGAA ACTTGCAGAT GAAGAAGCAC TGAGGAAAAT TAAGATGGCT 
GAGGAACAAA TAGACCATTT ACAGAGGAAA CTGACTGCCA CCAAACAGGA GGAGGAGGCC 
TTACTGTCAG AGATGGACGT GACGGGCCAG GCGTTTGAGG ACATGCAAGA ACAGAATATT 
CGTCTGATGC AGCAGCTCCG TGAGAAGGAC GACGCCAACT TCAAACTGAT GTCCGAGAGG 
ATCAAATCCA ACCAGATACA CAAACTCCTG AGGGAAGAGA AGGAGGAGCT GGCTGAGCAA 
GTGCAAGGGC TGAAAGCCCA GGTGGATGCT CAGCTGTTAC AGGTACAGAA ACTGGAGGAG 
AGAGAGCGCC TCTTACACAC GTCTTTGACT TCCATTGAGA AGGAAGTCAC TCTTAGAACA 
CAGTCACTGG AACTGAACAA GAGGAAGGCA GTCGAGGCAG CCCAGCTCGC CGAAGACCTG 
AAAGTGCAAA TGGAGCATGT AGGGGAAACG CTTAGGGATA CCCAGAACTT TGTATGTGAG 
AACCGAAGCG CTAAAGAAAA GGAGTCCTTT AGTCTCAAAA GAGCTCAGGA GGATGTATCT 
CGGCTGCGAA GGAAACTGGA AAAGCAGAAG AAGATGGAGG TTTATGCAGA TGCTGACCAA 
ATCCTACAGG AAGAGATTAA AGAGTACAGG GCCCGACTCA CTTGCCCTTG CTGTAACACT 
CGGAAAAAGG ACGCCGTCCT CACCAAGTGC TTTCACGTCT TCTGCTTCGA GTGCGTGAAG 
ACCCGATACG AGTCCCGGCA GCGCAAGTGC CCCAAGTGCA ACGCAGCCTT CGGAGCCCAC 
GACTTTCACC GGATTTACAT CAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918163.1
CDS	172..3138
Translation	MSGAGSKRASSDNGSGPSEKKPNLEAKTPTTLIEPIRLGGISSTEEMDMKVLQFKNKKLAERLEQRQGFEDELREKIEKLEKRQATDDATLLIVNRYWSKLDEDVHVLLKRYDSEPSKQSAAPSAATEEPESAKETAMETDESAEGSQPPPTQEIKAASPEPPPAPPFSTGALPESSLTFLATLAHCTKEEIELKLQERMEFSKKAVSCVLETSNKLHCRTEELCDRIHKGDFDHLGESPKKLNKELITENRRLQDLATVLQEKHHRLSLQHHELQDRATSTETKVSEMERTIEDLQWDIEKLRKHEQKLNRHLAEALEQLNSGYHISGSSGSFQGGQTTLSMQKFEMLNAELDQNQELANSRISELEKLQNELQAAVRLKEKLKLDLRTLPEEAVRNTQDFRCLQSQFSLLYNESLQVKTQLDEARALLLSTKNSHLRQIERMESEELSVQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLIGSLQNHNHQLKGDVQRYKRKVRDSQAELRFQIGGPPCPQPGSSGSEPTTPVTVKEEEVEVKKEPLAVVPREPETPTTPEVKKEEEEQKKEAAERTKAREREAERERDKDRERDKEKEREREKDKQKTDESKRKDCDILKQLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKTKAEMEEQRTRVQALEEKDKRERKKLADEEALRKIKMAEEQIDHLQRKLTATKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLREEKEELAEQVQGLKAQVDAQLLQVQKLEERERLLHTSLTSIEKEVTLRTQSLELNKRKAVEAAQLAEDLKVQMEHVGETLRDTQNFVCENRSAKEKESFSLKRAQEDVSRLRRKLEKQKKMEVYADADQILQEEIKEYRARLTCPCCNTRKKDAVLTKCFHVFCFECVKTRYESRQRKCPKCNAAFGAHDFHRIYIN

Target ORF information:

RefSeq Version	XM_004918106.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918106.3

ORF Insert Sequence:

ATGTCAGGGG CCGGAAGTAA GCGAGCCTCG AGCGACAATG GCTCTGGCCC TTCAGAAAAG 
AAACCAAATC TTGAAGCCAA GACGCCCACT ACTTTAATTG AGCCCATACG ACTAGGGGGC 
ATCTCCTCAA CAGAGGAGAT GGATATGAAA GTCCTGCAGT TCAAGAACAA GAAACTGGCG 
GAACGCCTGG AGCAGAGGCA GGGCTTTGAA GATGAACTCC GGGAGAAGAT CGAGAAGCTG 
GAGAAAAGAC AGGCTACTGA CGACGCTACT TTGCTCATTG TGAACCGTTA TTGGAGCAAG 
CTGGATGAGG ATGTTCACGT GCTGCTAAAG CGTTATGATT CCGAGCCAAG TAAGCAGAGT 
GCTGCGCCTA GCGCTGCTAC CGAGGAACCA GAGAGTGCCA AGGAGACTGC CATGGAAACA 
GACGAGAGTG CAGAGGGCTC ACAGCCCCCA CCAACCCAAG AAATCAAAGC AGCTTCCCCG 
GAACCTCCCC CCGCTCCTCC TTTCTCTACC GGAGCGTTGC CAGAATCTTC TTTGACCTTC 
TTAGCCACAT TAGCCCACTG TACCAAAGAG GAGATTGAGC TGAAGCTTCA AGAACGCATG 
GAGTTTAGCA AGAAGGCTGT ATCCTGTGTT CTGGAAACTT CTAACAAGCT CCACTGCCGA 
ACGGAAGAGC TATGTGACCG AATCCACAAA GGCGATTTCG ATCACTTGGG GGAATCGCCT 
AAAAAACTGA ACAAAGAACT TATCACTGAG AACCGTAGGC TGCAGGACCT CGCTACTGTG 
CTACAGGAGA AGCATCACCG ACTCTCCCTG CAGCATCATG AGCTCCAGGA CCGTGCAACT 
TCAACAGAGA CCAAGGTGTC CGAGATGGAG AGGACCATTG AGGACCTGCA GTGGGACATT 
GAGAAATTAC GCAAGCATGA ACAGAAGCTA AACCGTCATC TGGCAGAGGC CCTGGAGCAG 
CTAAATTCTG GATACCACAT CTCTGGAAGT TCTGGAAGTT TCCAGGGAGG ACAGACTACC 
CTAAGTATGC AAAAGTTTGA AATGTTGAAT GCAGAACTCG ATCAGAACCA GGAACTTGCA 
AACAGTCGGA TTTCCGAGCT GGAAAAACTG CAGAATGAAC TACAGGCAGC TGTCAGGCTA 
AAGGAGAAGC TAAAGCTGGA CCTGCGGACA TTGCCTGAGG AAGCGGTACG AAACACGCAG 
GATTTCCGCT GCTTGCAGTC TCAATTCTCT CTCCTGTACA ATGAGTCTCT GCAAGTAAAG 
ACCCAGTTGG ATGAGGCCCG AGCGCTGCTG TTAAGCACCA AGAACAGCCA CCTGAGACAG 
ATTGAACGCA TGGAGAGCGA GGAGCTGTCT GTACAAAAGA AACTGCGCAC TGAAGTGATC 
CAGTTGGAGG ATACGCTCGC CCAGGTGCGC AAAGAATATG AAATGCTGAG GATAGAGTTT 
GAGCAGAACC TGGCAGCCAA TGAGCAAGCA GGTCCTATTA ATAGGGAGAT GCGGCATCTC 
ATTGGAAGCC TCCAAAACCA CAACCACCAA CTGAAGGGAG ATGTGCAAAG ATACAAGCGC 
AAGGTGCGAG ATTCCCAGGC AGAGCTGCGT TTTCAAATCG GCGGACCACC CTGTCCTCAG 
CCTGGATCTT CAGGATCGGA ACCAACGACC CCCGTTACTG TCAAAGAGGA AGAAGTAGAA 
GTGAAGAAAG AACCCCTTGC AGTCGTGCCA CGAGAGCCTG AGACTCCCAC TACCCCTGAA 
GTAAAAAAGG AGGAGGAAGA GCAAAAGAAA GAGGCAGCAG AGCGGACCAA AGCCCGGGAG 
AGGGAGGCGG AACGGGAGCG AGACAAAGAT CGGGAAAGAG ACAAGGAAAA GGAAAGGGAG 
CGTGAGAAAG ACAAGCAGAA AACAGATGAG AGCAAAAGGA AAGACTGCGA CATTCTGAAA 
CAGCTCCGGG CAGAGCTCAA GAAGGCCCAG GAGAGCCAGA AAGAGATGAA GCTGCTGCTC 
GATATGTACA AATCTGCCCC CAAAGAGCAG AGAGACAAGG TGCAGCTGAT GGCAGCGGAG 
AGAAAGACCA AGGCCGAGAT GGAGGAGCAG CGCACGCGGG TGCAAGCTCT TGAGGAGAAA 
GACAAGAGGG AGAGGAAGAA ACTTGCAGAT GAAGAAGCAC TGAGGAAAAT TAAGATGGCT 
GAGGAACAAA TAGACCATTT ACAGAGGAAA CTGACTGCCA CCAAACAGGA GGAGGAGGCC 
TTACTGTCAG AGATGGACGT GACGGGCCAG GCGTTTGAGG ACATGCAAGA ACAGAATATT 
CGTCTGATGC AGCAGCTCCG TGAGAAGGAC GACGCCAACT TCAAACTGAT GTCCGAGAGG 
ATCAAATCCA ACCAGATACA CAAACTCCTG AGGGAAGAGA AGGAGGAGCT GGCTGAGCAA 
GTGCAAGGGC TGAAAGCCCA GGTGGATGCT CAGCTGTTAC AGGTACAGAA ACTGGAGGAG 
AGAGAGCGCC TCTTACACAC GTCTTTGACT TCCATTGAGA AGGAAGTCAC TCTTAGAACA 
CAGTCACTGG AACTGAACAA GAGGAAGGCA GTCGAGGCAG CCCAGCTCGC CGAAGACCTG 
AAAGTGCAAA TGGAGCATGT AGGGGAAACG CTTAGGGATA CCCAGAACTT TGTATGTGAG 
AACCGAAGCG CTAAAGAAAA GGAGTCCTTT AGTCTCAAAA GAGCTCAGGA GGATGTATCT 
CGGCTGCGAA GGAAACTGGA AAAGCAGAAG AAGATGGAGG TTTATGCAGA TGCTGACCAA 
ATCCTACAGG AAGAGATTAA AGAGTACAGG GCCCGACTCA CTTGCCCTTG CTGTAACACT 
CGGAAAAAGG ACGCCGTCCT CACCAAGTGC TTTCACGTCT TCTGCTTCGA GTGCGTGAAG 
ACCCGATACG AGTCCCGGCA GCGCAAGTGC CCCAAGTGCA ACGCAGCCTT CGGAGCCCAC 
GACTTTCACC GGATTTACAT CAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rnf40 ( XM_002940582.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940628.1 Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa16540
Clone ID Related Accession (Same CDS sequence)	XM_002940582.4 , XM_002940582.5
Accession Version	XM_002940582.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase BRE1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940582.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGGG CCGGAAGTAA GCGAGCCTCG AGCGACAATG GCTCTGGCCC TTCAGAAAAG 
AAACCAAATC TTGAAGCCAA GACGCCCACT ACTTTAATTG AGCCCATACG ACTAGGGGGC 
ATCTCCTCAA CAGAGGAGAT GGATATGAAA GTCCTGCAGT TCAAGAACAA GAAACTGGCG 
GAACGCCTGG AGCAGAGGCA GGGCTTTGAA GATGAACTCC GGGAGAAGAT CGAGAAGCTG 
GAGAAAAGAC AGGCTACTGA CGACGCTACT TTGCTCATTG TGAACCGTTA TTGGAGCAAG 
CTGGATGAGG ATGTTCACGT GCTGCTAAAG CGTTATGATT CCGAGCCAAG TAAGCAGAGT 
GCTGCGCCTA GCGCTGCTAC CGAGGAACCA GAGAGTGCCA AGGAGACTGC CATGGAAACA 
GACGAGAGTG CAGAGGGCTC ACAGCCCCCA CCAACCCAAG AAATCAAAGC AGCTTCCCCG 
GAACCTCCCC CCGCTCCTCC TTTCTCTACC GGAGCGTTGC CAGAATCTTC TTTGACCTTC 
TTAGCCACAT TAGCCCACTG TACCAAAGAG GAGATTGAGC TGAAGCTTCA AGAACGCATG 
GAGTTTAGCA AGAAGGCTGT ATCCTGTGTT CTGGAAACTT CTAACAAGCT CCACTGCCGA 
ACGGAAGAGC TATGTGACCG AATCCACAAA GGCGATTTCG ATCACTTGGG GGAATCGCCT 
AAAAAACTGA ACAAAGAACT TATCACTGAG AACCGTAGGC TGCAGGACCT CGCTACTGTG 
CTACAGGAGA AGCATCACCG ACTCTCCCTG CAGCATCATG AGCTCCAGGA CCGTGCAACT 
TCAACAGAGA CCAAGGTGTC CGAGATGGAG AGGACCATTG AGGACCTGCA GTGGGACATT 
GAGAAATTAC GCAAGCATGA ACAGAAGCTA AACCGTCATC TGGCAGAGGC CCTGGAGCAG 
CTAAATTCTG GATACCACAT CTCTGGAAGT TCTGGAAGTT TCCAGGGAGG ACAGACTACC 
CTAAGTATGC AAAAGTTTGA AATGTTGAAT GCAGAACTCG ATCAGAACCA GGAACTTGCA 
AACAGTCGGA TTTCCGAGCT GGAAAAACTG CAGAATGAAC TACAGGCAGC TGTCAGGCTA 
AAGGAGAAGC TAAAGCTGGA CCTGCGGACA TTGCCTGAGG AAGCGGTACG AAACACGCAG 
GATTTCCGCT GCTTGCAGTC TCAATTCTCT CTCCTGTACA ATGAGTCTCT GCAAGTAAAG 
ACCCAGTTGG ATGAGGCCCG AGCGCTGCTG TTAAGCACCA AGAACAGCCA CCTGAGACAG 
ATTGAACGCA TGGAGAGCGA GGAGCTGTCT GTACAAAAGA AACTGCGCAC TGAAGTGATC 
CAGTTGGAGG ATACGCTCGC CCAGGTGCGC AAAGAATATG AAATGCTGAG GATAGAGTTT 
GAGCAGAACC TGGCAGCCAA TGAGCAAGCA GGTCCTATTA ATAGGGAGAT GCGGCATCTC 
ATTGGAAGCC TCCAAAACCA CAACCACCAA CTGAAGGGAG ATGTGCAAAG ATACAAGCGC 
AAGGTGCGAG ATTCCCAGGC AGAGGTGAGC AAGCTGCGTT TTCAAATCGG CGGACCACCC 
TGTCCTCAGC CTGGATCTTC AGGATCGGAA CCAACGACCC CCGTTACTGT CAAAGAGGAA 
GAAGTAGAAG TGAAGAAAGA ACCCCTTGCA GTCGTGCCAC GAGAGCCTGA GACTCCCACT 
ACCCCTGAAG TAAAAAAGGA GGAGGAAGAG CAAAAGAAAG AGGCAGCAGA GCGGACCAAA 
GCCCGGGAGA GGGAGGCGGA ACGGGAGCGA GACAAAGATC GGGAAAGAGA CAAGGAAAAG 
GAAAGGGAGC GTGAGAAAGA CAAGCAGAAA ACAGATGAGA GCAAAAGGAA AGACTGCGAC 
ATTCTGAAAC AGCTCCGGGC AGAGCTCAAG AAGGCCCAGG AGAGCCAGAA AGAGATGAAG 
CTGCTGCTCG ATATGTACAA ATCTGCCCCC AAAGAGCAGA GAGACAAGGT GCAGCTGATG 
GCAGCGGAGA GAAAGACCAA GGCCGAGATG GAGGAGCAGC GCACGCGGGT GCAAGCTCTT 
GAGGAGAAAG ACAAGAGGGA GAGGAAGAAA CTTGCAGATG AAGAAGCACT GAGGAAAATT 
AAGATGGCTG AGGAACAAAT AGACCATTTA CAGAGGAAAC TGACTGCCAC CAAACAGGAG 
GAGGAGGCCT TACTGTCAGA GATGGACGTG ACGGGCCAGG CGTTTGAGGA CATGCAAGAA 
CAGAATATTC GTCTGATGCA GCAGCTCCGT GAGAAGGACG ACGCCAACTT CAAACTGATG 
TCCGAGAGGA TCAAATCCAA CCAGATACAC AAACTCCTGA GGGAAGAGAA GGAGGAGCTG 
GCTGAGCAAG TGCAAGGGCT GAAAGCCCAG GTGGATGCTC AGCTGTTACA GGTACAGAAA 
CTGGAGGAGA GAGAGCGCCT CTTACACACG TCTTTGACTT CCATTGAGAA GGAAGTCACT 
CTTAGAACAC AGTCACTGGA ACTGAACAAG AGGAAGGCAG TCGAGGCAGC CCAGCTCGCC 
GAAGACCTGA AAGTGCAAAT GGAGCATGTA GGGGAAACGC TTAGGGATAC CCAGAACTTT 
GTATGTGAGA ACCGAAGCGC TAAAGAAAAG GAGTCCTTTA GTCTCAAAAG AGCTCAGGAG 
GATGTATCTC GGCTGCGAAG GAAACTGGAA AAGCAGAAGA AGATGGAGGT TTATGCAGAT 
GCTGACCAAA TCCTACAGGA AGAGATTAAA GAGTACAGGG CCCGACTCAC TTGCCCTTGC 
TGTAACACTC GGAAAAAGGA CGCCGTCCTC ACCAAGTGCT TTCACGTCTT CTGCTTCGAG 
TGCGTGAAGA CCCGATACGA GTCCCGGCAG CGCAAGTGCC CCAAGTGCAA CGCAGCCTTC 
GGAGCCCACG ACTTTCACCG GATTTACATC AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940628.1
CDS	203..3178
Translation	MSGAGSKRASSDNGSGPSEKKPNLEAKTPTTLIEPIRLGGISSTEEMDMKVLQFKNKKLAERLEQRQGFEDELREKIEKLEKRQATDDATLLIVNRYWSKLDEDVHVLLKRYDSEPSKQSAAPSAATEEPESAKETAMETDESAEGSQPPPTQEIKAASPEPPPAPPFSTGALPESSLTFLATLAHCTKEEIELKLQERMEFSKKAVSCVLETSNKLHCRTEELCDRIHKGDFDHLGESPKKLNKELITENRRLQDLATVLQEKHHRLSLQHHELQDRATSTETKVSEMERTIEDLQWDIEKLRKHEQKLNRHLAEALEQLNSGYHISGSSGSFQGGQTTLSMQKFEMLNAELDQNQELANSRISELEKLQNELQAAVRLKEKLKLDLRTLPEEAVRNTQDFRCLQSQFSLLYNESLQVKTQLDEARALLLSTKNSHLRQIERMESEELSVQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLIGSLQNHNHQLKGDVQRYKRKVRDSQAEVSKLRFQIGGPPCPQPGSSGSEPTTPVTVKEEEVEVKKEPLAVVPREPETPTTPEVKKEEEEQKKEAAERTKAREREAERERDKDRERDKEKEREREKDKQKTDESKRKDCDILKQLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKTKAEMEEQRTRVQALEEKDKRERKKLADEEALRKIKMAEEQIDHLQRKLTATKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLREEKEELAEQVQGLKAQVDAQLLQVQKLEERERLLHTSLTSIEKEVTLRTQSLELNKRKAVEAAQLAEDLKVQMEHVGETLRDTQNFVCENRSAKEKESFSLKRAQEDVSRLRRKLEKQKKMEVYADADQILQEEIKEYRARLTCPCCNTRKKDAVLTKCFHVFCFECVKTRYESRQRKCPKCNAAFGAHDFHRIYIN

Target ORF information:

RefSeq Version	XM_002940582.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940582.5

ORF Insert Sequence:

ATGTCAGGGG CCGGAAGTAA GCGAGCCTCG AGCGACAATG GCTCTGGCCC TTCAGAAAAG 
AAACCAAATC TTGAAGCCAA GACGCCCACT ACTTTAATTG AGCCCATACG ACTAGGGGGC 
ATCTCCTCAA CAGAGGAGAT GGATATGAAA GTCCTGCAGT TCAAGAACAA GAAACTGGCG 
GAACGCCTGG AGCAGAGGCA GGGCTTTGAA GATGAACTCC GGGAGAAGAT CGAGAAGCTG 
GAGAAAAGAC AGGCTACTGA CGACGCTACT TTGCTCATTG TGAACCGTTA TTGGAGCAAG 
CTGGATGAGG ATGTTCACGT GCTGCTAAAG CGTTATGATT CCGAGCCAAG TAAGCAGAGT 
GCTGCGCCTA GCGCTGCTAC CGAGGAACCA GAGAGTGCCA AGGAGACTGC CATGGAAACA 
GACGAGAGTG CAGAGGGCTC ACAGCCCCCA CCAACCCAAG AAATCAAAGC AGCTTCCCCG 
GAACCTCCCC CCGCTCCTCC TTTCTCTACC GGAGCGTTGC CAGAATCTTC TTTGACCTTC 
TTAGCCACAT TAGCCCACTG TACCAAAGAG GAGATTGAGC TGAAGCTTCA AGAACGCATG 
GAGTTTAGCA AGAAGGCTGT ATCCTGTGTT CTGGAAACTT CTAACAAGCT CCACTGCCGA 
ACGGAAGAGC TATGTGACCG AATCCACAAA GGCGATTTCG ATCACTTGGG GGAATCGCCT 
AAAAAACTGA ACAAAGAACT TATCACTGAG AACCGTAGGC TGCAGGACCT CGCTACTGTG 
CTACAGGAGA AGCATCACCG ACTCTCCCTG CAGCATCATG AGCTCCAGGA CCGTGCAACT 
TCAACAGAGA CCAAGGTGTC CGAGATGGAG AGGACCATTG AGGACCTGCA GTGGGACATT 
GAGAAATTAC GCAAGCATGA ACAGAAGCTA AACCGTCATC TGGCAGAGGC CCTGGAGCAG 
CTAAATTCTG GATACCACAT CTCTGGAAGT TCTGGAAGTT TCCAGGGAGG ACAGACTACC 
CTAAGTATGC AAAAGTTTGA AATGTTGAAT GCAGAACTCG ATCAGAACCA GGAACTTGCA 
AACAGTCGGA TTTCCGAGCT GGAAAAACTG CAGAATGAAC TACAGGCAGC TGTCAGGCTA 
AAGGAGAAGC TAAAGCTGGA CCTGCGGACA TTGCCTGAGG AAGCGGTACG AAACACGCAG 
GATTTCCGCT GCTTGCAGTC TCAATTCTCT CTCCTGTACA ATGAGTCTCT GCAAGTAAAG 
ACCCAGTTGG ATGAGGCCCG AGCGCTGCTG TTAAGCACCA AGAACAGCCA CCTGAGACAG 
ATTGAACGCA TGGAGAGCGA GGAGCTGTCT GTACAAAAGA AACTGCGCAC TGAAGTGATC 
CAGTTGGAGG ATACGCTCGC CCAGGTGCGC AAAGAATATG AAATGCTGAG GATAGAGTTT 
GAGCAGAACC TGGCAGCCAA TGAGCAAGCA GGTCCTATTA ATAGGGAGAT GCGGCATCTC 
ATTGGAAGCC TCCAAAACCA CAACCACCAA CTGAAGGGAG ATGTGCAAAG ATACAAGCGC 
AAGGTGCGAG ATTCCCAGGC AGAGGTGAGC AAGCTGCGTT TTCAAATCGG CGGACCACCC 
TGTCCTCAGC CTGGATCTTC AGGATCGGAA CCAACGACCC CCGTTACTGT CAAAGAGGAA 
GAAGTAGAAG TGAAGAAAGA ACCCCTTGCA GTCGTGCCAC GAGAGCCTGA GACTCCCACT 
ACCCCTGAAG TAAAAAAGGA GGAGGAAGAG CAAAAGAAAG AGGCAGCAGA GCGGACCAAA 
GCCCGGGAGA GGGAGGCGGA ACGGGAGCGA GACAAAGATC GGGAAAGAGA CAAGGAAAAG 
GAAAGGGAGC GTGAGAAAGA CAAGCAGAAA ACAGATGAGA GCAAAAGGAA AGACTGCGAC 
ATTCTGAAAC AGCTCCGGGC AGAGCTCAAG AAGGCCCAGG AGAGCCAGAA AGAGATGAAG 
CTGCTGCTCG ATATGTACAA ATCTGCCCCC AAAGAGCAGA GAGACAAGGT GCAGCTGATG 
GCAGCGGAGA GAAAGACCAA GGCCGAGATG GAGGAGCAGC GCACGCGGGT GCAAGCTCTT 
GAGGAGAAAG ACAAGAGGGA GAGGAAGAAA CTTGCAGATG AAGAAGCACT GAGGAAAATT 
AAGATGGCTG AGGAACAAAT AGACCATTTA CAGAGGAAAC TGACTGCCAC CAAACAGGAG 
GAGGAGGCCT TACTGTCAGA GATGGACGTG ACGGGCCAGG CGTTTGAGGA CATGCAAGAA 
CAGAATATTC GTCTGATGCA GCAGCTCCGT GAGAAGGACG ACGCCAACTT CAAACTGATG 
TCCGAGAGGA TCAAATCCAA CCAGATACAC AAACTCCTGA GGGAAGAGAA GGAGGAGCTG 
GCTGAGCAAG TGCAAGGGCT GAAAGCCCAG GTGGATGCTC AGCTGTTACA GGTACAGAAA 
CTGGAGGAGA GAGAGCGCCT CTTACACACG TCTTTGACTT CCATTGAGAA GGAAGTCACT 
CTTAGAACAC AGTCACTGGA ACTGAACAAG AGGAAGGCAG TCGAGGCAGC CCAGCTCGCC 
GAAGACCTGA AAGTGCAAAT GGAGCATGTA GGGGAAACGC TTAGGGATAC CCAGAACTTT 
GTATGTGAGA ACCGAAGCGC TAAAGAAAAG GAGTCCTTTA GTCTCAAAAG AGCTCAGGAG 
GATGTATCTC GGCTGCGAAG GAAACTGGAA AAGCAGAAGA AGATGGAGGT TTATGCAGAT 
GCTGACCAAA TCCTACAGGA AGAGATTAAA GAGTACAGGG CCCGACTCAC TTGCCCTTGC 
TGTAACACTC GGAAAAAGGA CGCCGTCCTC ACCAAGTGCT TTCACGTCTT CTGCTTCGAG 
TGCGTGAAGA CCCGATACGA GTCCCGGCAG CGCAAGTGCC CCAAGTGCAA CGCAGCCTTC 
GGAGCCCACG ACTTTCACCG GATTTACATC AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rnf40 ( XM_004918105.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918162.1 Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16541
Clone ID Related Accession (Same CDS sequence)	XM_004918105.4 , XM_004918105.3
Accession Version	XM_004918105.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase BRE1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004918105.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGGG CCGGAAGTAA GCGAGCCTCG AGCGACAATG GCTCTGGCCC TTCAGAAAAG 
AAACCAAATC TTGAAGCCAA GACGCCCACT ACTTTAATTG AGCCCATACG ACTAGGGGGC 
ATCTCCTCAA CAGAGGAGAT GGATATGAAA GTCCTGCAGT TCAAGAACAA GAAACTGGCG 
GAACGCCTGG AGCAGAGGCA GGGCTTTGAA GATGAACTCC GGGAGAAGAT CGAGAAGCTG 
GAGAAAAGAC AGGCTACTGA CGACGCTACT TTGCTCATTG TGAACCGTTA TTGGAGCAAG 
CTGGATGAGG ATGTTCACGT GCTGCTAAAG CGTTATGATT CCGAGCCAAG TAAGCAGAGT 
GCTGCGCCTA GCGCTGCTAC CGAGGAACCA GAGAGTGCCA AGGAGACTGC CATGGAAACA 
GACGAGAGTG CAGAGGGCTC ACAGCCCCCA CCAACCCAAG AAATCAAAGC TTCCCCGGAA 
CCTCCCCCCG CTCCTCCTTT CTCTACCGGA GCGTTGCCAG AATCTTCTTT GACCTTCTTA 
GCCACATTAG CCCACTGTAC CAAAGAGGAG ATTGAGCTGA AGCTTCAAGA ACGCATGGAG 
TTTAGCAAGA AGGCTGTATC CTGTGTTCTG GAAACTTCTA ACAAGCTCCA CTGCCGAACG 
GAAGAGCTAT GTGACCGAAT CCACAAAGGC GATTTCGATC ACTTGGGGGA ATCGCCTAAA 
AAACTGAACA AAGAACTTAT CACTGAGAAC CGTAGGCTGC AGGACCTCGC TACTGTGCTA 
CAGGAGAAGC ATCACCGACT CTCCCTGCAG CATCATGAGC TCCAGGACCG TGCAACTTCA 
ACAGAGACCA AGGTGTCCGA GATGGAGAGG ACCATTGAGG ACCTGCAGTG GGACATTGAG 
AAATTACGCA AGCATGAACA GAAGCTAAAC CGTCATCTGG CAGAGGCCCT GGAGCAGCTA 
AATTCTGGAT ACCACATCTC TGGAAGTTCT GGAAGTTTCC AGGGAGGACA GACTACCCTA 
AGTATGCAAA AGTTTGAAAT GTTGAATGCA GAACTCGATC AGAACCAGGA ACTTGCAAAC 
AGTCGGATTT CCGAGCTGGA AAAACTGCAG AATGAACTAC AGGCAGCTGT CAGGCTAAAG 
GAGAAGCTAA AGCTGGACCT GCGGACATTG CCTGAGGAAG CGGTACGAAA CACGCAGGAT 
TTCCGCTGCT TGCAGTCTCA ATTCTCTCTC CTGTACAATG AGTCTCTGCA AGTAAAGACC 
CAGTTGGATG AGGCCCGAGC GCTGCTGTTA AGCACCAAGA ACAGCCACCT GAGACAGATT 
GAACGCATGG AGAGCGAGGA GCTGTCTGTA CAAAAGAAAC TGCGCACTGA AGTGATCCAG 
TTGGAGGATA CGCTCGCCCA GGTGCGCAAA GAATATGAAA TGCTGAGGAT AGAGTTTGAG 
CAGAACCTGG CAGCCAATGA GCAAGCAGGT CCTATTAATA GGGAGATGCG GCATCTCATT 
GGAAGCCTCC AAAACCACAA CCACCAACTG AAGGGAGATG TGCAAAGATA CAAGCGCAAG 
GTGCGAGATT CCCAGGCAGA GGTGAGCAAG CTGCGTTTTC AAATCGGCGG ACCACCCTGT 
CCTCAGCCTG GATCTTCAGG ATCGGAACCA ACGACCCCCG TTACTGTCAA AGAGGAAGAA 
GTAGAAGTGA AGAAAGAACC CCTTGCAGTC GTGCCACGAG AGCCTGAGAC TCCCACTACC 
CCTGAAGTAA AAAAGGAGGA GGAAGAGCAA AAGAAAGAGG CAGCAGAGCG GACCAAAGCC 
CGGGAGAGGG AGGCGGAACG GGAGCGAGAC AAAGATCGGG AAAGAGACAA GGAAAAGGAA 
AGGGAGCGTG AGAAAGACAA GCAGAAAACA GATGAGAGCA AAAGGAAAGA CTGCGACATT 
CTGAAACAGC TCCGGGCAGA GCTCAAGAAG GCCCAGGAGA GCCAGAAAGA GATGAAGCTG 
CTGCTCGATA TGTACAAATC TGCCCCCAAA GAGCAGAGAG ACAAGGTGCA GCTGATGGCA 
GCGGAGAGAA AGACCAAGGC CGAGATGGAG GAGCAGCGCA CGCGGGTGCA AGCTCTTGAG 
GAGAAAGACA AGAGGGAGAG GAAGAAACTT GCAGATGAAG AAGCACTGAG GAAAATTAAG 
ATGGCTGAGG AACAAATAGA CCATTTACAG AGGAAACTGA CTGCCACCAA ACAGGAGGAG 
GAGGCCTTAC TGTCAGAGAT GGACGTGACG GGCCAGGCGT TTGAGGACAT GCAAGAACAG 
AATATTCGTC TGATGCAGCA GCTCCGTGAG AAGGACGACG CCAACTTCAA ACTGATGTCC 
GAGAGGATCA AATCCAACCA GATACACAAA CTCCTGAGGG AAGAGAAGGA GGAGCTGGCT 
GAGCAAGTGC AAGGGCTGAA AGCCCAGGTG GATGCTCAGC TGTTACAGGT ACAGAAACTG 
GAGGAGAGAG AGCGCCTCTT ACACACGTCT TTGACTTCCA TTGAGAAGGA AGTCACTCTT 
AGAACACAGT CACTGGAACT GAACAAGAGG AAGGCAGTCG AGGCAGCCCA GCTCGCCGAA 
GACCTGAAAG TGCAAATGGA GCATGTAGGG GAAACGCTTA GGGATACCCA GAACTTTGTA 
TGTGAGAACC GAAGCGCTAA AGAAAAGGAG TCCTTTAGTC TCAAAAGAGC TCAGGAGGAT 
GTATCTCGGC TGCGAAGGAA ACTGGAAAAG CAGAAGAAGA TGGAGGTTTA TGCAGATGCT 
GACCAAATCC TACAGGAAGA GATTAAAGAG TACAGGGCCC GACTCACTTG CCCTTGCTGT 
AACACTCGGA AAAAGGACGC CGTCCTCACC AAGTGCTTTC ACGTCTTCTG CTTCGAGTGC 
GTGAAGACCC GATACGAGTC CCGGCAGCGC AAGTGCCCCA AGTGCAACGC AGCCTTCGGA 
GCCCACGACT TTCACCGGAT TTACATCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918162.1
CDS	203..3175
Translation	MSGAGSKRASSDNGSGPSEKKPNLEAKTPTTLIEPIRLGGISSTEEMDMKVLQFKNKKLAERLEQRQGFEDELREKIEKLEKRQATDDATLLIVNRYWSKLDEDVHVLLKRYDSEPSKQSAAPSAATEEPESAKETAMETDESAEGSQPPPTQEIKASPEPPPAPPFSTGALPESSLTFLATLAHCTKEEIELKLQERMEFSKKAVSCVLETSNKLHCRTEELCDRIHKGDFDHLGESPKKLNKELITENRRLQDLATVLQEKHHRLSLQHHELQDRATSTETKVSEMERTIEDLQWDIEKLRKHEQKLNRHLAEALEQLNSGYHISGSSGSFQGGQTTLSMQKFEMLNAELDQNQELANSRISELEKLQNELQAAVRLKEKLKLDLRTLPEEAVRNTQDFRCLQSQFSLLYNESLQVKTQLDEARALLLSTKNSHLRQIERMESEELSVQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLIGSLQNHNHQLKGDVQRYKRKVRDSQAEVSKLRFQIGGPPCPQPGSSGSEPTTPVTVKEEEVEVKKEPLAVVPREPETPTTPEVKKEEEEQKKEAAERTKAREREAERERDKDRERDKEKEREREKDKQKTDESKRKDCDILKQLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKTKAEMEEQRTRVQALEEKDKRERKKLADEEALRKIKMAEEQIDHLQRKLTATKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLREEKEELAEQVQGLKAQVDAQLLQVQKLEERERLLHTSLTSIEKEVTLRTQSLELNKRKAVEAAQLAEDLKVQMEHVGETLRDTQNFVCENRSAKEKESFSLKRAQEDVSRLRRKLEKQKKMEVYADADQILQEEIKEYRARLTCPCCNTRKKDAVLTKCFHVFCFECVKTRYESRQRKCPKCNAAFGAHDFHRIYIN

Target ORF information:

RefSeq Version	XM_004918105.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918105.4

ORF Insert Sequence:

ATGTCAGGGG CCGGAAGTAA GCGAGCCTCG AGCGACAATG GCTCTGGCCC TTCAGAAAAG 
AAACCAAATC TTGAAGCCAA GACGCCCACT ACTTTAATTG AGCCCATACG ACTAGGGGGC 
ATCTCCTCAA CAGAGGAGAT GGATATGAAA GTCCTGCAGT TCAAGAACAA GAAACTGGCG 
GAACGCCTGG AGCAGAGGCA GGGCTTTGAA GATGAACTCC GGGAGAAGAT CGAGAAGCTG 
GAGAAAAGAC AGGCTACTGA CGACGCTACT TTGCTCATTG TGAACCGTTA TTGGAGCAAG 
CTGGATGAGG ATGTTCACGT GCTGCTAAAG CGTTATGATT CCGAGCCAAG TAAGCAGAGT 
GCTGCGCCTA GCGCTGCTAC CGAGGAACCA GAGAGTGCCA AGGAGACTGC CATGGAAACA 
GACGAGAGTG CAGAGGGCTC ACAGCCCCCA CCAACCCAAG AAATCAAAGC TTCCCCGGAA 
CCTCCCCCCG CTCCTCCTTT CTCTACCGGA GCGTTGCCAG AATCTTCTTT GACCTTCTTA 
GCCACATTAG CCCACTGTAC CAAAGAGGAG ATTGAGCTGA AGCTTCAAGA ACGCATGGAG 
TTTAGCAAGA AGGCTGTATC CTGTGTTCTG GAAACTTCTA ACAAGCTCCA CTGCCGAACG 
GAAGAGCTAT GTGACCGAAT CCACAAAGGC GATTTCGATC ACTTGGGGGA ATCGCCTAAA 
AAACTGAACA AAGAACTTAT CACTGAGAAC CGTAGGCTGC AGGACCTCGC TACTGTGCTA 
CAGGAGAAGC ATCACCGACT CTCCCTGCAG CATCATGAGC TCCAGGACCG TGCAACTTCA 
ACAGAGACCA AGGTGTCCGA GATGGAGAGG ACCATTGAGG ACCTGCAGTG GGACATTGAG 
AAATTACGCA AGCATGAACA GAAGCTAAAC CGTCATCTGG CAGAGGCCCT GGAGCAGCTA 
AATTCTGGAT ACCACATCTC TGGAAGTTCT GGAAGTTTCC AGGGAGGACA GACTACCCTA 
AGTATGCAAA AGTTTGAAAT GTTGAATGCA GAACTCGATC AGAACCAGGA ACTTGCAAAC 
AGTCGGATTT CCGAGCTGGA AAAACTGCAG AATGAACTAC AGGCAGCTGT CAGGCTAAAG 
GAGAAGCTAA AGCTGGACCT GCGGACATTG CCTGAGGAAG CGGTACGAAA CACGCAGGAT 
TTCCGCTGCT TGCAGTCTCA ATTCTCTCTC CTGTACAATG AGTCTCTGCA AGTAAAGACC 
CAGTTGGATG AGGCCCGAGC GCTGCTGTTA AGCACCAAGA ACAGCCACCT GAGACAGATT 
GAACGCATGG AGAGCGAGGA GCTGTCTGTA CAAAAGAAAC TGCGCACTGA AGTGATCCAG 
TTGGAGGATA CGCTCGCCCA GGTGCGCAAA GAATATGAAA TGCTGAGGAT AGAGTTTGAG 
CAGAACCTGG CAGCCAATGA GCAAGCAGGT CCTATTAATA GGGAGATGCG GCATCTCATT 
GGAAGCCTCC AAAACCACAA CCACCAACTG AAGGGAGATG TGCAAAGATA CAAGCGCAAG 
GTGCGAGATT CCCAGGCAGA GGTGAGCAAG CTGCGTTTTC AAATCGGCGG ACCACCCTGT 
CCTCAGCCTG GATCTTCAGG ATCGGAACCA ACGACCCCCG TTACTGTCAA AGAGGAAGAA 
GTAGAAGTGA AGAAAGAACC CCTTGCAGTC GTGCCACGAG AGCCTGAGAC TCCCACTACC 
CCTGAAGTAA AAAAGGAGGA GGAAGAGCAA AAGAAAGAGG CAGCAGAGCG GACCAAAGCC 
CGGGAGAGGG AGGCGGAACG GGAGCGAGAC AAAGATCGGG AAAGAGACAA GGAAAAGGAA 
AGGGAGCGTG AGAAAGACAA GCAGAAAACA GATGAGAGCA AAAGGAAAGA CTGCGACATT 
CTGAAACAGC TCCGGGCAGA GCTCAAGAAG GCCCAGGAGA GCCAGAAAGA GATGAAGCTG 
CTGCTCGATA TGTACAAATC TGCCCCCAAA GAGCAGAGAG ACAAGGTGCA GCTGATGGCA 
GCGGAGAGAA AGACCAAGGC CGAGATGGAG GAGCAGCGCA CGCGGGTGCA AGCTCTTGAG 
GAGAAAGACA AGAGGGAGAG GAAGAAACTT GCAGATGAAG AAGCACTGAG GAAAATTAAG 
ATGGCTGAGG AACAAATAGA CCATTTACAG AGGAAACTGA CTGCCACCAA ACAGGAGGAG 
GAGGCCTTAC TGTCAGAGAT GGACGTGACG GGCCAGGCGT TTGAGGACAT GCAAGAACAG 
AATATTCGTC TGATGCAGCA GCTCCGTGAG AAGGACGACG CCAACTTCAA ACTGATGTCC 
GAGAGGATCA AATCCAACCA GATACACAAA CTCCTGAGGG AAGAGAAGGA GGAGCTGGCT 
GAGCAAGTGC AAGGGCTGAA AGCCCAGGTG GATGCTCAGC TGTTACAGGT ACAGAAACTG 
GAGGAGAGAG AGCGCCTCTT ACACACGTCT TTGACTTCCA TTGAGAAGGA AGTCACTCTT 
AGAACACAGT CACTGGAACT GAACAAGAGG AAGGCAGTCG AGGCAGCCCA GCTCGCCGAA 
GACCTGAAAG TGCAAATGGA GCATGTAGGG GAAACGCTTA GGGATACCCA GAACTTTGTA 
TGTGAGAACC GAAGCGCTAA AGAAAAGGAG TCCTTTAGTC TCAAAAGAGC TCAGGAGGAT 
GTATCTCGGC TGCGAAGGAA ACTGGAAAAG CAGAAGAAGA TGGAGGTTTA TGCAGATGCT 
GACCAAATCC TACAGGAAGA GATTAAAGAG TACAGGGCCC GACTCACTTG CCCTTGCTGT 
AACACTCGGA AAAAGGACGC CGTCCTCACC AAGTGCTTTC ACGTCTTCTG CTTCGAGTGC 
GTGAAGACCC GATACGAGTC CCGGCAGCGC AAGTGCCCCA AGTGCAACGC AGCCTTCGGA 
GCCCACGACT TTCACCGGAT TTACATCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rnf40 ( XM_004918106.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004918163.1 Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa16542
Clone ID Related Accession (Same CDS sequence)	XM_004918106.4 , XM_004918106.3
Accession Version	XM_004918106.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase BRE1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004918106.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGGG CCGGAAGTAA GCGAGCCTCG AGCGACAATG GCTCTGGCCC TTCAGAAAAG 
AAACCAAATC TTGAAGCCAA GACGCCCACT ACTTTAATTG AGCCCATACG ACTAGGGGGC 
ATCTCCTCAA CAGAGGAGAT GGATATGAAA GTCCTGCAGT TCAAGAACAA GAAACTGGCG 
GAACGCCTGG AGCAGAGGCA GGGCTTTGAA GATGAACTCC GGGAGAAGAT CGAGAAGCTG 
GAGAAAAGAC AGGCTACTGA CGACGCTACT TTGCTCATTG TGAACCGTTA TTGGAGCAAG 
CTGGATGAGG ATGTTCACGT GCTGCTAAAG CGTTATGATT CCGAGCCAAG TAAGCAGAGT 
GCTGCGCCTA GCGCTGCTAC CGAGGAACCA GAGAGTGCCA AGGAGACTGC CATGGAAACA 
GACGAGAGTG CAGAGGGCTC ACAGCCCCCA CCAACCCAAG AAATCAAAGC AGCTTCCCCG 
GAACCTCCCC CCGCTCCTCC TTTCTCTACC GGAGCGTTGC CAGAATCTTC TTTGACCTTC 
TTAGCCACAT TAGCCCACTG TACCAAAGAG GAGATTGAGC TGAAGCTTCA AGAACGCATG 
GAGTTTAGCA AGAAGGCTGT ATCCTGTGTT CTGGAAACTT CTAACAAGCT CCACTGCCGA 
ACGGAAGAGC TATGTGACCG AATCCACAAA GGCGATTTCG ATCACTTGGG GGAATCGCCT 
AAAAAACTGA ACAAAGAACT TATCACTGAG AACCGTAGGC TGCAGGACCT CGCTACTGTG 
CTACAGGAGA AGCATCACCG ACTCTCCCTG CAGCATCATG AGCTCCAGGA CCGTGCAACT 
TCAACAGAGA CCAAGGTGTC CGAGATGGAG AGGACCATTG AGGACCTGCA GTGGGACATT 
GAGAAATTAC GCAAGCATGA ACAGAAGCTA AACCGTCATC TGGCAGAGGC CCTGGAGCAG 
CTAAATTCTG GATACCACAT CTCTGGAAGT TCTGGAAGTT TCCAGGGAGG ACAGACTACC 
CTAAGTATGC AAAAGTTTGA AATGTTGAAT GCAGAACTCG ATCAGAACCA GGAACTTGCA 
AACAGTCGGA TTTCCGAGCT GGAAAAACTG CAGAATGAAC TACAGGCAGC TGTCAGGCTA 
AAGGAGAAGC TAAAGCTGGA CCTGCGGACA TTGCCTGAGG AAGCGGTACG AAACACGCAG 
GATTTCCGCT GCTTGCAGTC TCAATTCTCT CTCCTGTACA ATGAGTCTCT GCAAGTAAAG 
ACCCAGTTGG ATGAGGCCCG AGCGCTGCTG TTAAGCACCA AGAACAGCCA CCTGAGACAG 
ATTGAACGCA TGGAGAGCGA GGAGCTGTCT GTACAAAAGA AACTGCGCAC TGAAGTGATC 
CAGTTGGAGG ATACGCTCGC CCAGGTGCGC AAAGAATATG AAATGCTGAG GATAGAGTTT 
GAGCAGAACC TGGCAGCCAA TGAGCAAGCA GGTCCTATTA ATAGGGAGAT GCGGCATCTC 
ATTGGAAGCC TCCAAAACCA CAACCACCAA CTGAAGGGAG ATGTGCAAAG ATACAAGCGC 
AAGGTGCGAG ATTCCCAGGC AGAGCTGCGT TTTCAAATCG GCGGACCACC CTGTCCTCAG 
CCTGGATCTT CAGGATCGGA ACCAACGACC CCCGTTACTG TCAAAGAGGA AGAAGTAGAA 
GTGAAGAAAG AACCCCTTGC AGTCGTGCCA CGAGAGCCTG AGACTCCCAC TACCCCTGAA 
GTAAAAAAGG AGGAGGAAGA GCAAAAGAAA GAGGCAGCAG AGCGGACCAA AGCCCGGGAG 
AGGGAGGCGG AACGGGAGCG AGACAAAGAT CGGGAAAGAG ACAAGGAAAA GGAAAGGGAG 
CGTGAGAAAG ACAAGCAGAA AACAGATGAG AGCAAAAGGA AAGACTGCGA CATTCTGAAA 
CAGCTCCGGG CAGAGCTCAA GAAGGCCCAG GAGAGCCAGA AAGAGATGAA GCTGCTGCTC 
GATATGTACA AATCTGCCCC CAAAGAGCAG AGAGACAAGG TGCAGCTGAT GGCAGCGGAG 
AGAAAGACCA AGGCCGAGAT GGAGGAGCAG CGCACGCGGG TGCAAGCTCT TGAGGAGAAA 
GACAAGAGGG AGAGGAAGAA ACTTGCAGAT GAAGAAGCAC TGAGGAAAAT TAAGATGGCT 
GAGGAACAAA TAGACCATTT ACAGAGGAAA CTGACTGCCA CCAAACAGGA GGAGGAGGCC 
TTACTGTCAG AGATGGACGT GACGGGCCAG GCGTTTGAGG ACATGCAAGA ACAGAATATT 
CGTCTGATGC AGCAGCTCCG TGAGAAGGAC GACGCCAACT TCAAACTGAT GTCCGAGAGG 
ATCAAATCCA ACCAGATACA CAAACTCCTG AGGGAAGAGA AGGAGGAGCT GGCTGAGCAA 
GTGCAAGGGC TGAAAGCCCA GGTGGATGCT CAGCTGTTAC AGGTACAGAA ACTGGAGGAG 
AGAGAGCGCC TCTTACACAC GTCTTTGACT TCCATTGAGA AGGAAGTCAC TCTTAGAACA 
CAGTCACTGG AACTGAACAA GAGGAAGGCA GTCGAGGCAG CCCAGCTCGC CGAAGACCTG 
AAAGTGCAAA TGGAGCATGT AGGGGAAACG CTTAGGGATA CCCAGAACTT TGTATGTGAG 
AACCGAAGCG CTAAAGAAAA GGAGTCCTTT AGTCTCAAAA GAGCTCAGGA GGATGTATCT 
CGGCTGCGAA GGAAACTGGA AAAGCAGAAG AAGATGGAGG TTTATGCAGA TGCTGACCAA 
ATCCTACAGG AAGAGATTAA AGAGTACAGG GCCCGACTCA CTTGCCCTTG CTGTAACACT 
CGGAAAAAGG ACGCCGTCCT CACCAAGTGC TTTCACGTCT TCTGCTTCGA GTGCGTGAAG 
ACCCGATACG AGTCCCGGCA GCGCAAGTGC CCCAAGTGCA ACGCAGCCTT CGGAGCCCAC 
GACTTTCACC GGATTTACAT CAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004918163.1
CDS	203..3169
Translation	MSGAGSKRASSDNGSGPSEKKPNLEAKTPTTLIEPIRLGGISSTEEMDMKVLQFKNKKLAERLEQRQGFEDELREKIEKLEKRQATDDATLLIVNRYWSKLDEDVHVLLKRYDSEPSKQSAAPSAATEEPESAKETAMETDESAEGSQPPPTQEIKAASPEPPPAPPFSTGALPESSLTFLATLAHCTKEEIELKLQERMEFSKKAVSCVLETSNKLHCRTEELCDRIHKGDFDHLGESPKKLNKELITENRRLQDLATVLQEKHHRLSLQHHELQDRATSTETKVSEMERTIEDLQWDIEKLRKHEQKLNRHLAEALEQLNSGYHISGSSGSFQGGQTTLSMQKFEMLNAELDQNQELANSRISELEKLQNELQAAVRLKEKLKLDLRTLPEEAVRNTQDFRCLQSQFSLLYNESLQVKTQLDEARALLLSTKNSHLRQIERMESEELSVQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLIGSLQNHNHQLKGDVQRYKRKVRDSQAELRFQIGGPPCPQPGSSGSEPTTPVTVKEEEVEVKKEPLAVVPREPETPTTPEVKKEEEEQKKEAAERTKAREREAERERDKDRERDKEKEREREKDKQKTDESKRKDCDILKQLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKTKAEMEEQRTRVQALEEKDKRERKKLADEEALRKIKMAEEQIDHLQRKLTATKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLREEKEELAEQVQGLKAQVDAQLLQVQKLEERERLLHTSLTSIEKEVTLRTQSLELNKRKAVEAAQLAEDLKVQMEHVGETLRDTQNFVCENRSAKEKESFSLKRAQEDVSRLRRKLEKQKKMEVYADADQILQEEIKEYRARLTCPCCNTRKKDAVLTKCFHVFCFECVKTRYESRQRKCPKCNAAFGAHDFHRIYIN

Target ORF information:

RefSeq Version	XM_004918106.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ring finger protein 40, E3 ubiquitin protein ligase (rnf40), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004918106.4

ORF Insert Sequence:

ATGTCAGGGG CCGGAAGTAA GCGAGCCTCG AGCGACAATG GCTCTGGCCC TTCAGAAAAG 
AAACCAAATC TTGAAGCCAA GACGCCCACT ACTTTAATTG AGCCCATACG ACTAGGGGGC 
ATCTCCTCAA CAGAGGAGAT GGATATGAAA GTCCTGCAGT TCAAGAACAA GAAACTGGCG 
GAACGCCTGG AGCAGAGGCA GGGCTTTGAA GATGAACTCC GGGAGAAGAT CGAGAAGCTG 
GAGAAAAGAC AGGCTACTGA CGACGCTACT TTGCTCATTG TGAACCGTTA TTGGAGCAAG 
CTGGATGAGG ATGTTCACGT GCTGCTAAAG CGTTATGATT CCGAGCCAAG TAAGCAGAGT 
GCTGCGCCTA GCGCTGCTAC CGAGGAACCA GAGAGTGCCA AGGAGACTGC CATGGAAACA 
GACGAGAGTG CAGAGGGCTC ACAGCCCCCA CCAACCCAAG AAATCAAAGC AGCTTCCCCG 
GAACCTCCCC CCGCTCCTCC TTTCTCTACC GGAGCGTTGC CAGAATCTTC TTTGACCTTC 
TTAGCCACAT TAGCCCACTG TACCAAAGAG GAGATTGAGC TGAAGCTTCA AGAACGCATG 
GAGTTTAGCA AGAAGGCTGT ATCCTGTGTT CTGGAAACTT CTAACAAGCT CCACTGCCGA 
ACGGAAGAGC TATGTGACCG AATCCACAAA GGCGATTTCG ATCACTTGGG GGAATCGCCT 
AAAAAACTGA ACAAAGAACT TATCACTGAG AACCGTAGGC TGCAGGACCT CGCTACTGTG 
CTACAGGAGA AGCATCACCG ACTCTCCCTG CAGCATCATG AGCTCCAGGA CCGTGCAACT 
TCAACAGAGA CCAAGGTGTC CGAGATGGAG AGGACCATTG AGGACCTGCA GTGGGACATT 
GAGAAATTAC GCAAGCATGA ACAGAAGCTA AACCGTCATC TGGCAGAGGC CCTGGAGCAG 
CTAAATTCTG GATACCACAT CTCTGGAAGT TCTGGAAGTT TCCAGGGAGG ACAGACTACC 
CTAAGTATGC AAAAGTTTGA AATGTTGAAT GCAGAACTCG ATCAGAACCA GGAACTTGCA 
AACAGTCGGA TTTCCGAGCT GGAAAAACTG CAGAATGAAC TACAGGCAGC TGTCAGGCTA 
AAGGAGAAGC TAAAGCTGGA CCTGCGGACA TTGCCTGAGG AAGCGGTACG AAACACGCAG 
GATTTCCGCT GCTTGCAGTC TCAATTCTCT CTCCTGTACA ATGAGTCTCT GCAAGTAAAG 
ACCCAGTTGG ATGAGGCCCG AGCGCTGCTG TTAAGCACCA AGAACAGCCA CCTGAGACAG 
ATTGAACGCA TGGAGAGCGA GGAGCTGTCT GTACAAAAGA AACTGCGCAC TGAAGTGATC 
CAGTTGGAGG ATACGCTCGC CCAGGTGCGC AAAGAATATG AAATGCTGAG GATAGAGTTT 
GAGCAGAACC TGGCAGCCAA TGAGCAAGCA GGTCCTATTA ATAGGGAGAT GCGGCATCTC 
ATTGGAAGCC TCCAAAACCA CAACCACCAA CTGAAGGGAG ATGTGCAAAG ATACAAGCGC 
AAGGTGCGAG ATTCCCAGGC AGAGCTGCGT TTTCAAATCG GCGGACCACC CTGTCCTCAG 
CCTGGATCTT CAGGATCGGA ACCAACGACC CCCGTTACTG TCAAAGAGGA AGAAGTAGAA 
GTGAAGAAAG AACCCCTTGC AGTCGTGCCA CGAGAGCCTG AGACTCCCAC TACCCCTGAA 
GTAAAAAAGG AGGAGGAAGA GCAAAAGAAA GAGGCAGCAG AGCGGACCAA AGCCCGGGAG 
AGGGAGGCGG AACGGGAGCG AGACAAAGAT CGGGAAAGAG ACAAGGAAAA GGAAAGGGAG 
CGTGAGAAAG ACAAGCAGAA AACAGATGAG AGCAAAAGGA AAGACTGCGA CATTCTGAAA 
CAGCTCCGGG CAGAGCTCAA GAAGGCCCAG GAGAGCCAGA AAGAGATGAA GCTGCTGCTC 
GATATGTACA AATCTGCCCC CAAAGAGCAG AGAGACAAGG TGCAGCTGAT GGCAGCGGAG 
AGAAAGACCA AGGCCGAGAT GGAGGAGCAG CGCACGCGGG TGCAAGCTCT TGAGGAGAAA 
GACAAGAGGG AGAGGAAGAA ACTTGCAGAT GAAGAAGCAC TGAGGAAAAT TAAGATGGCT 
GAGGAACAAA TAGACCATTT ACAGAGGAAA CTGACTGCCA CCAAACAGGA GGAGGAGGCC 
TTACTGTCAG AGATGGACGT GACGGGCCAG GCGTTTGAGG ACATGCAAGA ACAGAATATT 
CGTCTGATGC AGCAGCTCCG TGAGAAGGAC GACGCCAACT TCAAACTGAT GTCCGAGAGG 
ATCAAATCCA ACCAGATACA CAAACTCCTG AGGGAAGAGA AGGAGGAGCT GGCTGAGCAA 
GTGCAAGGGC TGAAAGCCCA GGTGGATGCT CAGCTGTTAC AGGTACAGAA ACTGGAGGAG 
AGAGAGCGCC TCTTACACAC GTCTTTGACT TCCATTGAGA AGGAAGTCAC TCTTAGAACA 
CAGTCACTGG AACTGAACAA GAGGAAGGCA GTCGAGGCAG CCCAGCTCGC CGAAGACCTG 
AAAGTGCAAA TGGAGCATGT AGGGGAAACG CTTAGGGATA CCCAGAACTT TGTATGTGAG 
AACCGAAGCG CTAAAGAAAA GGAGTCCTTT AGTCTCAAAA GAGCTCAGGA GGATGTATCT 
CGGCTGCGAA GGAAACTGGA AAAGCAGAAG AAGATGGAGG TTTATGCAGA TGCTGACCAA 
ATCCTACAGG AAGAGATTAA AGAGTACAGG GCCCGACTCA CTTGCCCTTG CTGTAACACT 
CGGAAAAAGG ACGCCGTCCT CACCAAGTGC TTTCACGTCT TCTGCTTCGA GTGCGTGAAG 
ACCCGATACG AGTCCCGGCA GCGCAAGTGC CCCAAGTGCA ACGCAGCCTT CGGAGCCCAC 
GACTTTCACC GGATTTACAT CAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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rnf40 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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