topaz1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following topaz1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the topaz1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa24852 NM_001199939.1
Latest version!
Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa44875 XM_018094575.1
Latest version!
Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa44876 XM_018094576.1
Latest version!
Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa59574 XM_031903275.1
Latest version!
Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa59574 XM_018094575.2
Latest version!
Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa59574 XM_031903276.1
Latest version!
Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa33111 NM_001374744.1
Latest version!
Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa33111 XM_012964409.2
Latest version!
Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OXa33112 XM_012964413.2
Latest version!
Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa33112 XM_012964412.2
Latest version!
Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OXa33112 XM_012964411.2
Latest version!
Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa24852
    Clone ID Related Accession (Same CDS sequence) NM_001199939.1
    Accession Version NM_001199939.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein TOPAZ1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL895749.2, EL833478.1, DN087003.1, BX756929.1, BX770256.1, DT402254.1, DT402253.1 and EL833479.1. On Jan 14, 2011 this sequence version replaced XM_002937848.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGGGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186868.1
    CDS189..2678
    Translation

    Target ORF information:

    RefSeq Version NM_001199939.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199939.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGGGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44875
    Clone ID Related Accession (Same CDS sequence) XM_018094575.1
    Accession Version XM_018094575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product testis- and ovary-specific PAZ domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950064.1
    CDS162..2651
    Translation

    Target ORF information:

    RefSeq Version XM_018094575.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa44876
    Clone ID Related Accession (Same CDS sequence) XM_018094576.1
    Accession Version XM_018094576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2457bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product testis- and ovary-specific PAZ domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACCTTTA TTAATGAGTG TAATGCAGGT GTGAATCAAC CCTACGTGGA CAGCAGAAAT 
    GCTGTGGATT CCCTTGTGTT TCAGAGCATA AGAGGAAAAA GGAGAGTGCA CAGCAGAAAA
    CTTTATGTTC TACACACAAC TGTTATTTCA CTGTTACAGA TCCAGCACAC ACGCACAAAT
    GATGCCAGCC CTGAGGAAAA TGTAGAAGAG AGAAGTACAT ATTTTGCAAA AGTAATCACT
    GATCTAGAAG TTAATGATGA TCCTCTCATC ATACATAACA CTTTGGAAAA GAACAGTGAA
    GATTTTTATG ATGGTGGACA GTTTGATTTT GAGGCGTTCA GAGATCTTGA CGCAGATAAA
    GAGATTAAAT ATGCTGAAAA GAATTCATCT GAAAGCGGAA GCAGTTTTGA AGAGTCCTTG
    TCCAAGGATA CTTCTCCGGG GTCCTCTCAT TCCAATAGCC GTCGTTGGAG AAGAGATCCC
    TCACATACCT ATAAGCCTAT GCATTGGATG AATGATTTCA GATACTCTGG AAAATGCATG
    GGATTGACGG ATGTGATTAC TTTGGATCAG AAAGAACAGA GGAATATTGA TAATCTCTTT
    TACGATTTTA CACCTATACA GAAGACCTTT TTGCCCATTA GATACTGCAA ATATTTTTTT
    AATACTTTCC GTGGATGCAT TAAACCAGAT TGTATGTACC AGCACGTACC ATTCCAAAGG
    GATGAAAAGG TGTGCATGGA GGTGATTCAC AAACTTGTGA ATGAAAATCA CACAACATTA
    TTAAAACGTG CTGTTTGGAT ATTTACTGCC TACTACAGAA TGTATATGCC TGGTGTTCAT
    TATGACTCAA ATCTGCTGAC CAAAATGCTC CGTGCCTTGT ATGTTCGTCA GATGTGGGGA
    GATGTATTTC AGTTGTTGGA AACTGGTGCC AATGTCAAAA TATTGCCCTC CTCTGAAATG
    CTTATCAGGT TATTTGAAAA TATTGGCTCT ACGGGATTAA CAGCAGCTGT ACCTAGTTTA
    GTGGATGTAT TCTGCAAGCT TGTGGAGGCT GGAATGATGA TGAAGCCAGA GCAAATCAAC
    ATGTTAATAA CAACACTGAA TAATCTCCAG GCAACAAAAA ATTATATCAG TGTCATTTTA
    GACATAAAAA CAAGAATTGA AATGCAGCTT TCAGAAAAGA ACTGGCTGTG TAACTTGGAT
    ATAGCAGTTG CAGAAGTTGG GCATTGTAAA GAAAAAAATG ATTGGAAGAA ACTAGGAACA
    TTATATCTTA ATCTGCGTAC AGGGTGTGAG AATGTGACTG ATCTTAAGAA GTTTTCTAAT
    TGCATTGTGG GGGCGCTTCA GAAAGTGCCA AGGAATGATA AATCTGAAAT TCCATATTGT
    GACTTTGCAG ACACAGTGTA CAAAGACGCC CAACTAAGTG AAATTGACAA AAACATTTTG
    GGAAGGATTG GAATATCCAT CATGTATCAC TATTACAGAA ATAAACAATC GCAAAAGGGA
    AAGAGGGTGC TCAGAAAACT TCAGGAAATG CAGATTAATT TCACAGTCCT AAAAGGACTC
    ACAGGGGAAG AGAGTAAAGC TACGCGCTGT CAAGTTGTGA ACACAGCTGT AGAGATCTTC
    TTGAACTGTG AATACTTAAA TGGTGCACTG GGGGTTCTAC GAGAGTCAGA GTGGATCATT
    AACACACAAG TGTGGCCATG TGAAAGAATG GATGTGCTGA AACGACATAA CCTGTTGTGT
    ACAATTGCAC AGAAAACACT GAATAAAAAC ATGTTTGATG TGTGTCTTGA GGTCCTTCAG
    AATCTGCCAG GACTGCAGTG CTCATTAACG GATGTGGACG TCTCTCAGTA CAGCTTGCTT
    TTCAACAAAC TTTTGTCATC CTGCATAGAA AATAATACAC TTGGTGTATC CTCAACTGTG
    ATAGATTTTA TGATTGCAAA AAAAATACCA CTTGACTTTT TCTTACTGAG AGCACTAATC
    ACATCCTTGG GACGAAGCTG TCTATGGCTC AGAGCAAGAG AACTTTATAA ATGCGCTGTT
    TTGTTGGGTT GCTACCCGCC CATGGAAGGA AATCTGTATC GTAAAGTGCT CTTCATCCCC
    TCATTCCTTA CTGAGATAGA AATGCTGTTA TCCATTGAAA TTTTCATGGT GTCAAATGCC
    AGTAGTATTC AGTCACCAGG AGGCAGCCAT CAGACTCTGC AAATCATTCT CAAAAGATGT
    GAAGAAGAGA GAGTTCCGAA TAAAGATTAT CAAAGAGGTA AAGCTAGCTA TCAAGATGCA
    GTGGACCGGC TGATTCAGGC CTCTAGGCTA TCAACACCAA GATTATTCAT AAAGCACCTG
    ACTGTCAATA ATGCCAATGA ACAGGTTTAT ATCCTTGATT ACGGCAGCTC TCTCAAATGG
    TTGCATGAAA ACATGAAATG GGCTGGGAAA GTATGGCTTT TTCAGGGGAA AATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950065.1
    CDS346..2802
    Translation

    Target ORF information:

    RefSeq Version XM_018094576.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGACCTTTA TTAATGAGTG TAATGCAGGT GTGAATCAAC CCTACGTGGA CAGCAGAAAT 
    GCTGTGGATT CCCTTGTGTT TCAGAGCATA AGAGGAAAAA GGAGAGTGCA CAGCAGAAAA
    CTTTATGTTC TACACACAAC TGTTATTTCA CTGTTACAGA TCCAGCACAC ACGCACAAAT
    GATGCCAGCC CTGAGGAAAA TGTAGAAGAG AGAAGTACAT ATTTTGCAAA AGTAATCACT
    GATCTAGAAG TTAATGATGA TCCTCTCATC ATACATAACA CTTTGGAAAA GAACAGTGAA
    GATTTTTATG ATGGTGGACA GTTTGATTTT GAGGCGTTCA GAGATCTTGA CGCAGATAAA
    GAGATTAAAT ATGCTGAAAA GAATTCATCT GAAAGCGGAA GCAGTTTTGA AGAGTCCTTG
    TCCAAGGATA CTTCTCCGGG GTCCTCTCAT TCCAATAGCC GTCGTTGGAG AAGAGATCCC
    TCACATACCT ATAAGCCTAT GCATTGGATG AATGATTTCA GATACTCTGG AAAATGCATG
    GGATTGACGG ATGTGATTAC TTTGGATCAG AAAGAACAGA GGAATATTGA TAATCTCTTT
    TACGATTTTA CACCTATACA GAAGACCTTT TTGCCCATTA GATACTGCAA ATATTTTTTT
    AATACTTTCC GTGGATGCAT TAAACCAGAT TGTATGTACC AGCACGTACC ATTCCAAAGG
    GATGAAAAGG TGTGCATGGA GGTGATTCAC AAACTTGTGA ATGAAAATCA CACAACATTA
    TTAAAACGTG CTGTTTGGAT ATTTACTGCC TACTACAGAA TGTATATGCC TGGTGTTCAT
    TATGACTCAA ATCTGCTGAC CAAAATGCTC CGTGCCTTGT ATGTTCGTCA GATGTGGGGA
    GATGTATTTC AGTTGTTGGA AACTGGTGCC AATGTCAAAA TATTGCCCTC CTCTGAAATG
    CTTATCAGGT TATTTGAAAA TATTGGCTCT ACGGGATTAA CAGCAGCTGT ACCTAGTTTA
    GTGGATGTAT TCTGCAAGCT TGTGGAGGCT GGAATGATGA TGAAGCCAGA GCAAATCAAC
    ATGTTAATAA CAACACTGAA TAATCTCCAG GCAACAAAAA ATTATATCAG TGTCATTTTA
    GACATAAAAA CAAGAATTGA AATGCAGCTT TCAGAAAAGA ACTGGCTGTG TAACTTGGAT
    ATAGCAGTTG CAGAAGTTGG GCATTGTAAA GAAAAAAATG ATTGGAAGAA ACTAGGAACA
    TTATATCTTA ATCTGCGTAC AGGGTGTGAG AATGTGACTG ATCTTAAGAA GTTTTCTAAT
    TGCATTGTGG GGGCGCTTCA GAAAGTGCCA AGGAATGATA AATCTGAAAT TCCATATTGT
    GACTTTGCAG ACACAGTGTA CAAAGACGCC CAACTAAGTG AAATTGACAA AAACATTTTG
    GGAAGGATTG GAATATCCAT CATGTATCAC TATTACAGAA ATAAACAATC GCAAAAGGGA
    AAGAGGGTGC TCAGAAAACT TCAGGAAATG CAGATTAATT TCACAGTCCT AAAAGGACTC
    ACAGGGGAAG AGAGTAAAGC TACGCGCTGT CAAGTTGTGA ACACAGCTGT AGAGATCTTC
    TTGAACTGTG AATACTTAAA TGGTGCACTG GGGGTTCTAC GAGAGTCAGA GTGGATCATT
    AACACACAAG TGTGGCCATG TGAAAGAATG GATGTGCTGA AACGACATAA CCTGTTGTGT
    ACAATTGCAC AGAAAACACT GAATAAAAAC ATGTTTGATG TGTGTCTTGA GGTCCTTCAG
    AATCTGCCAG GACTGCAGTG CTCATTAACG GATGTGGACG TCTCTCAGTA CAGCTTGCTT
    TTCAACAAAC TTTTGTCATC CTGCATAGAA AATAATACAC TTGGTGTATC CTCAACTGTG
    ATAGATTTTA TGATTGCAAA AAAAATACCA CTTGACTTTT TCTTACTGAG AGCACTAATC
    ACATCCTTGG GACGAAGCTG TCTATGGCTC AGAGCAAGAG AACTTTATAA ATGCGCTGTT
    TTGTTGGGTT GCTACCCGCC CATGGAAGGA AATCTGTATC GTAAAGTGCT CTTCATCCCC
    TCATTCCTTA CTGAGATAGA AATGCTGTTA TCCATTGAAA TTTTCATGGT GTCAAATGCC
    AGTAGTATTC AGTCACCAGG AGGCAGCCAT CAGACTCTGC AAATCATTCT CAAAAGATGT
    GAAGAAGAGA GAGTTCCGAA TAAAGATTAT CAAAGAGGTA AAGCTAGCTA TCAAGATGCA
    GTGGACCGGC TGATTCAGGC CTCTAGGCTA TCAACACCAA GATTATTCAT AAAGCACCTG
    ACTGTCAATA ATGCCAATGA ACAGGTTTAT ATCCTTGATT ACGGCAGCTC TCTCAAATGG
    TTGCATGAAA ACATGAAATG GGCTGGGAAA GTATGGCTTT TTCAGGGGAA AATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59574
    Clone ID Related Accession (Same CDS sequence) XM_031903275.1 , XM_018094575.2 , XM_031903276.1
    Accession Version XM_031903275.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein TOPAZ1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACACAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TCGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC CTGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ATGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTTCTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759135.1
    CDS152..2641
    Translation

    Target ORF information:

    RefSeq Version XM_031903275.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903275.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACACAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TCGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC CTGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ATGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTTCTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59574
    Clone ID Related Accession (Same CDS sequence) XM_031903275.1 , XM_018094575.2 , XM_031903276.1
    Accession Version XM_018094575.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein TOPAZ1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACACAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TCGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC CTGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ATGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTTCTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950064.2
    CDS161..2650
    Translation

    Target ORF information:

    RefSeq Version XM_018094575.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094575.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACACAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TCGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC CTGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ATGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTTCTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa59574
    Clone ID Related Accession (Same CDS sequence) XM_031903275.1 , XM_018094575.2 , XM_031903276.1
    Accession Version XM_031903276.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product protein TOPAZ1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACACAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TCGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC CTGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ATGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTTCTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031759136.1
    CDS149..2638
    Translation

    Target ORF information:

    RefSeq Version XM_031903276.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031903276.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACACAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TCGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC CTGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ATGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTTCTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33111
    Clone ID Related Accession (Same CDS sequence) XM_012964409.2 , NM_001374744.1
    Accession Version NM_001374744.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-09
    Organism Xenopus tropicalis(tropical clawed frog)
    Product testis- and ovary-specific PAZ domain-containing protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03023896.1. On Oct 10, 2019 this sequence version replaced XM_012964409.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00862000, SAMN04027313 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGAGTATGT TGCACTCTAG AAAAATTAAG CTAGTTCAAC CTAACCGGTC TGAATCCAGC 
    TCATTTGCTA AGATCTGTGA TGAGGCTGAT ACTGAAAAAA GTAGTTCAGT TCTTATCAGT
    CCAGCTCTCT CTCTACAGTA TTCCTCAAAA GCCATTGATC AGGATAGGAG TGTAAAGAAT
    CTGTCAGGTA GAAACCCAAA GGCCATAAGT GGTGTGAATG AGCCTTGTGC AATCCAGCAA
    TGTGGAAATA TAGCGGTGTC TACAGAATCT GACTTTATTA GAATGAATGA CACAAGGAAG
    GCAAGACTAG CACTTTCAGA TCCAGTTCTT GCTTCTAATG ACTCTGGAGA AAGTATTAAT
    GTTTCAAACT TGCCGTCTTG TCAAGTTAAA AGAAGAACCA GAAGGAAGGC AACTGATCTG
    CGCGGTGCGG AGTCAATTGA AAAAGAAAAG TGTGGAAGTT TAGTAGTAAA TCAGGAAGCA
    GCAATTGTGA ATACTGATAC TACTGTTCCA CTAGATATTG AGAATGTTTA CAGTCCTACT
    CATATGCTAA ATCAACGGGG ATTGTTGAAA AAAATAGGAA AGAGGCCAAC AATTAAGAAG
    GAAGATGTGA GTAGTGTAGG AGATAGACAA AGATGTTTAA AACTAGCAGG AAAGCAAAGT
    GGAAAGAAAC TGCAATCTGA GCAAAGTGTT GTGTGTGGCA AGGGAAAGAC TCAGGAATGT
    GTACAAATAA TAGGGAAAAA AAGAGGAAGG AGGGCAAAAA CTGATCAGAG TAGTGAGTGT
    GGCATAAGTG AGATTCAGCA AAGAAAGAAA AGTAGAAAAA AGGTAAAAAC TGATAAGAGT
    GATGTGTGTT GTTCAGGAGA GACATTGAAA TATGTTAATA AAATTAAAAG AGGGAGGAAA
    CTAAAAACTG ATCATAAAGT TGTAAGTAGC AAAGGAGAAA CACAGGAATG TGTAAAATTA
    ACAGACATGA ATCAGAAACC AAAAACTGAA TGTTTTGCTG TGTGTGGCAC AGAAGAGACT
    CAGGCTTGTG TAAAACCACT GGGAAAGAAA AAAGGCAGGA TACTAGGTAA TAGTGTTGGT
    AAAAATAAAA TTAATGCTGG GTGTTATTCA GAATTAGATA AAATGCGTAC CGTATATGTT
    AGGGTACAGC GATTGTCATT GAATAAAATT CCCCAGAGTG CAGGAATCTC CACAGAACAT
    CTGAGGAGTG TGCTGCTTCT GAAGTCTGAG TATAGACAGG AATGTGGGGA AGCAAGAACA
    GAAGAGAGGA ATGATGCTAC AGATGTGACC TTCTTAAAAA AGAATCTCAG GAGTCCTAGA
    CAGCAGAAAT GCTGTGGATT CCCTTGTGTT TCAGAGCATA AGAGGAAAAA GGAGAGTGCA
    CAGCAGAAAA CTTTATGTTC TACACACAAC TGTTATTTCA CTGTTACAGA TCCAGCACAC
    ACGGTAAATG GAATAACGTG CATGCAAAAT CCATTGCTTG AAACAGCAAA CAATAGAAAA
    AGTAAATGTT CATATGGTGG TTCCCAGTCT TTTCTAAATC CTGTAGTGAA ATTGCATAAC
    TGCAGCAACA TGAAGAGGCT TATACGTTGC TCAAATCTGA GGGTAAGCAG TTCCAAAACT
    GAGAATACAT GTCCTGCAGC AACAGTAAAA GAACTTGATC CTCAAAAACA ATTACCTTGT
    TCTGTTAACA CTGTAAATTC AGTTATCAGT ACAGATTCTA CTGTTGGAGG TAATCATTCC
    AAAGAACTGG AATCTATTGC ACATGGCTTG GCAAATAGGA AAATTAAAAG AATATTAAAA
    GTAAAGTGGG ACTTTGGAAA AAAAATAAGG AATTCTGCAA CATGCTCTAC AGAGCGTACT
    CAAGAAGACT CTGCAATATC TGTCAAACAG AAAACAGGAG ACCTAAAATA TAGTGGTAAT
    CCTACTGTCA ACACAGACTC ATCAGATCCT TGCATTGGTA CTTTGGAAAA GATTATTGAC
    GAGATGAACA ATAATAAAAG TGACTCTGTA AATTTTAACT TGAAGGATGC CCTTTGTGAT
    CAAAGCACAA GTCAAAACAA AAAGGAAAGT TATGATTGCC TTCCGGTACA ATGCAGGACT
    ACACAATTAC CGTTTATGGT TTCAGAGTGT GAAAGAAAGG ATAGGGAATC CCTTGACATA
    GAGTGTTCTG CACTTATTAG AATGTCTGCA AATGAGTCAG TGGTCGATGA CTATTCAGAT
    TTTTCACTTA CTGAGGAAAA TGTTAACTTT GTAAATAATA ACAATATAAA TGAGCATACA
    GAAGAATGCA AAACCTGTTC CTGTCAGAGA ACAATCCCAT TTACTGGGAA AACCATCTGG
    AAATGTTCTT GTGCAAGGAC GTGTGTATGG GCTTTCCCAA GAAAAAGAAC TTCCTCTTTA
    CCAAGTACAG ATATACTGGG ATTTGACTCT GAATTGTCTT TTGCCCCTTA TTCCACCAAT
    ATAAAGTCTT TATTTCAGGC TAATGAAGCA GAGCCATTGC AGAGCACAAC TGATGACACT
    GACAGTGACG GGATATGTAA AAAACTAAAG TTAGATTTCA TTTCAGAAAA TGACGCTCTT
    ATTCTTAAAG ATATTGATGA TGACATTTTG TCTCACCCTC CAACAGAAAC TTTTGAACTA
    CCCAATACTG TAATTGAGAA GATATGTAAT ATAACTGAAA ATGTTTACTC ACTAGGTGAA
    GTTGAAGACA TGTATAAAAA TTTAGATGGT TGGCCATGCA GTTCAACTAT TATTCAAAAT
    ACTATAAATA GTCTTCCTCT CCCGCTGCCA AACAATGACA AAAGTGAGCA CTTTACCACT
    TCTGGCACTT ATTCTGAAAT AGAACCTACT GTAAAAACAG TTGAGGAGAT GGCCCTGAGC
    AGTTCTTTTG AAAACTCTGA TCCAAGTGTT TGTTCCAGAG ACAGCTCCAC AGAACCTGAA
    AACAGTTCAG AGACTGTGCT AAAAAGTACT CAATATGAAT CTCCAATACG TGATTGTTTA
    GACAAGCATA AAAAAAACAC TAATGTTGAG GAAGTGTTAT CCTTTTTTGA TTGTTTACAT
    GAAACTACAA TGGATAACGC CTGTCTAGCT GAGGAAAATT GCACCAGACA GATTAATAAT
    ACTGGTGGCC CATTAGATGA TTCACATTTG ACACTAAGCC ATGTAGAATC TCCAAAGAAA
    AGCAAGCTTG TGGAATACAA AAGTAATTTA GATGTGATAA AAGCTTATGA AGAAGATGTA
    CTGTTTTTAG ATGTTGTTCC AGATGATCCA GAGCTTTTTG GGACTCCTGA AGATCCTTAC
    ATAAGTCCAA TTTGTAATGC TGACAACAAA GAAAATAATA AACGGAATAA TCTGAGTGCA
    TTAATGTGTA ACCTTCAGGA ACAGGAGAGC GCCCATATCA ATGAAAGCAC AAATGATGCC
    AGCCCTGAGG AAAATGTAGA AGAGAGAAGT ACATATTTTG CAAAAGTAAT CACTGATCTA
    GAAGTTAATG ATGATCCTCT CATCATACAT AACACTTTGG AAAAGAACAG TGAAGATTTT
    TATGATGGTG GACAGTTTGA TTTTGAGGCG TTCAGAGATC TTGACGCAGA TAAAGAGATT
    AAATATGCTG AAAAGAATTC ATCTGAAAGC GGAAGCAGTT TTGAAGAGTC CTTGTCCAAG
    GATACTTCTC CGGGGTCCTC TCATTCCAAT AGCCGTCGTT GGAGAAGAGA TCCCTCACAT
    ACCTATAAGC CTATGCATTG GATGAATGAT TTCAGATACT CTGGAAAATG CATGGGATTG
    ACGGATGTGA TTACTTTGGA TCAGAAAGAA CAGAGGAATA TTGATAATCT CTTTTACGAT
    TTTACACCTA TACAGAAGAC CTTTTTGCCC ATTAGATACT GCAAATATTT TTTTAATACT
    TTCCGTGGAT GCATTAAACC AGATTGTATG TACCAGCACG TACCATTCCA AAGGGATGAA
    AAGGTGTGCA TGGAGGTGAT TCACAAACTT GTGAATGAAA ATCACACAAC ATTATTAAAA
    CGTGCTGTTT GGATATTTAC TGCCTACTAC AGAATGTATA TGCCTGGTGT TCATTATGAC
    TCAAATCTGC TGACCAAAAT GCTCCGTGCC TTGTATGTTC GTCAGATGTG GGGAGATGTA
    TTTCAGTTGT TGGAAACTGG TGCCAATGTC AAAATATTGC CCTCCTCTGA AATGCTTATC
    AGGTTATTTG AAAATATTGG CTCTACGGGA TTAACAGCAG CTGTACCTAG TTTAGTGGAT
    GTATTCTGCA AGCTTGTGGA GGCTGGAATG ATGATGAAGC CAGAGCAAAT CAACATGTTA
    ATAACAACAC TGAATAATCT CCAGGCAACA AAAAATTATA TCAGTGTCAT TTTAGACATA
    AAAACAAGAA TTGAAATGCA GCTTTCAGAA AAGAACTGGC TGTGTAACTT GGATATAGCA
    GTTGCAGAAG TTGGGCATTG TAAAGAAAAA AATGATTGGA AGAAACTAGG AACATTATAT
    CTTAATCTGC GTACAGGGTG TGAGAATGTG ACTGATCTTA AGAAGTTTTC TAATTGCATT
    GTGGGGGCGC TTCAGAAAGT GCCAAGGAAT GATAAATCTG AAATTCCATA TTGTGACTTT
    GCAGACACAG TGTACAAAGA CGCCCAACTA AGTGAAATTG ACAAAAACAT TTTGGGAAGG
    ATTGGAATAT CCATCATGTA TCACTATTAC AGAAATAAAC AATCGCAAAA GGGAAAGAGG
    GTGCTCAGAA AACTTCAGGA AATGCAGATT AATTTCACAG TCCTAAAAGG ACTCACAGGG
    GAAGAGAGTA AAGCTACGCG CTGTCAAGTT GTGAACACAG CTGTAGAGAT CTTCTTGAAC
    TGTGAATACT TAAATGGTGC ACTGGGGGTT CTACGAGAGT CAGAGTGGAT CATTAACACA
    CAAGTGTGGC CATGTGAAAG AATGGATGTG CTGAAACGAC ATAACCTGTT GTGTACAATT
    GCACAGAAAA CACTGAATAA AAACATGTTT GATGTGTGTC TTGAGGTCCT TCAGAATCTG
    CCAGGACTGC AGTGCTCATT AACGGATGTG GACGTCTCTC AGTACAGCTT GCTTTTCAAC
    AAACTTTTGT CATCCTGCAT AGAAAATAAT ACACTTGGTG TATCCTCAAC TGTGATAGAT
    TTTATGATTG CAAAAAAAAT ACCACTTGAC TTTTTCTTAC TGAGAGCACT AATCACATCC
    TTGGGACGAA GCTGTCTATG GCTCAGAGCA AGAGAACTTT ATAAATGCGC TGTTTTGTTG
    GGTTGCTACC CGCCCATGGA AGGAAATCTG TATCGTAAAG TGCTCTTCAT CCCCTCATTC
    CTTACTGAGA TAGAAATGCT GTTATCCATT GAAATTTTCA TGGTGTCAAA TGCCAGTAGT
    ATTCAGTCAC CAGGAGGCAG CCATCAGACT CTGCAAATCA TTCTCAAAAG ATGTGAAGAA
    GAGAGAGTTC CGAATAAAGA TTATCAAAGA GGTAAAGCTA GCTATCAAGA TGCAGTGGAC
    CGGCTGATTC AGGCCTCTAG GCTATCAACA CCAAGATTAT TCATAAAGCA CCTGACTGTC
    AATAATGCCA ATGAACAGGT TTATATCCTT GATTACGGCA GCTCTCTCAA ATGGTTGCAT
    GAAAACATGA AATGGGCTGG GAAAGTATGG CTTTTTCAGG GGAAAATATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001361673.1
    CDS777..6467
    Translation

    Target ORF information:

    RefSeq Version NM_001374744.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001374744.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGAGTATGT TGCACTCTAG AAAAATTAAG CTAGTTCAAC CTAACCGGTC TGAATCCAGC 
    TCATTTGCTA AGATCTGTGA TGAGGCTGAT ACTGAAAAAA GTAGTTCAGT TCTTATCAGT
    CCAGCTCTCT CTCTACAGTA TTCCTCAAAA GCCATTGATC AGGATAGGAG TGTAAAGAAT
    CTGTCAGGTA GAAACCCAAA GGCCATAAGT GGTGTGAATG AGCCTTGTGC AATCCAGCAA
    TGTGGAAATA TAGCGGTGTC TACAGAATCT GACTTTATTA GAATGAATGA CACAAGGAAG
    GCAAGACTAG CACTTTCAGA TCCAGTTCTT GCTTCTAATG ACTCTGGAGA AAGTATTAAT
    GTTTCAAACT TGCCGTCTTG TCAAGTTAAA AGAAGAACCA GAAGGAAGGC AACTGATCTG
    CGCGGTGCGG AGTCAATTGA AAAAGAAAAG TGTGGAAGTT TAGTAGTAAA TCAGGAAGCA
    GCAATTGTGA ATACTGATAC TACTGTTCCA CTAGATATTG AGAATGTTTA CAGTCCTACT
    CATATGCTAA ATCAACGGGG ATTGTTGAAA AAAATAGGAA AGAGGCCAAC AATTAAGAAG
    GAAGATGTGA GTAGTGTAGG AGATAGACAA AGATGTTTAA AACTAGCAGG AAAGCAAAGT
    GGAAAGAAAC TGCAATCTGA GCAAAGTGTT GTGTGTGGCA AGGGAAAGAC TCAGGAATGT
    GTACAAATAA TAGGGAAAAA AAGAGGAAGG AGGGCAAAAA CTGATCAGAG TAGTGAGTGT
    GGCATAAGTG AGATTCAGCA AAGAAAGAAA AGTAGAAAAA AGGTAAAAAC TGATAAGAGT
    GATGTGTGTT GTTCAGGAGA GACATTGAAA TATGTTAATA AAATTAAAAG AGGGAGGAAA
    CTAAAAACTG ATCATAAAGT TGTAAGTAGC AAAGGAGAAA CACAGGAATG TGTAAAATTA
    ACAGACATGA ATCAGAAACC AAAAACTGAA TGTTTTGCTG TGTGTGGCAC AGAAGAGACT
    CAGGCTTGTG TAAAACCACT GGGAAAGAAA AAAGGCAGGA TACTAGGTAA TAGTGTTGGT
    AAAAATAAAA TTAATGCTGG GTGTTATTCA GAATTAGATA AAATGCGTAC CGTATATGTT
    AGGGTACAGC GATTGTCATT GAATAAAATT CCCCAGAGTG CAGGAATCTC CACAGAACAT
    CTGAGGAGTG TGCTGCTTCT GAAGTCTGAG TATAGACAGG AATGTGGGGA AGCAAGAACA
    GAAGAGAGGA ATGATGCTAC AGATGTGACC TTCTTAAAAA AGAATCTCAG GAGTCCTAGA
    CAGCAGAAAT GCTGTGGATT CCCTTGTGTT TCAGAGCATA AGAGGAAAAA GGAGAGTGCA
    CAGCAGAAAA CTTTATGTTC TACACACAAC TGTTATTTCA CTGTTACAGA TCCAGCACAC
    ACGGTAAATG GAATAACGTG CATGCAAAAT CCATTGCTTG AAACAGCAAA CAATAGAAAA
    AGTAAATGTT CATATGGTGG TTCCCAGTCT TTTCTAAATC CTGTAGTGAA ATTGCATAAC
    TGCAGCAACA TGAAGAGGCT TATACGTTGC TCAAATCTGA GGGTAAGCAG TTCCAAAACT
    GAGAATACAT GTCCTGCAGC AACAGTAAAA GAACTTGATC CTCAAAAACA ATTACCTTGT
    TCTGTTAACA CTGTAAATTC AGTTATCAGT ACAGATTCTA CTGTTGGAGG TAATCATTCC
    AAAGAACTGG AATCTATTGC ACATGGCTTG GCAAATAGGA AAATTAAAAG AATATTAAAA
    GTAAAGTGGG ACTTTGGAAA AAAAATAAGG AATTCTGCAA CATGCTCTAC AGAGCGTACT
    CAAGAAGACT CTGCAATATC TGTCAAACAG AAAACAGGAG ACCTAAAATA TAGTGGTAAT
    CCTACTGTCA ACACAGACTC ATCAGATCCT TGCATTGGTA CTTTGGAAAA GATTATTGAC
    GAGATGAACA ATAATAAAAG TGACTCTGTA AATTTTAACT TGAAGGATGC CCTTTGTGAT
    CAAAGCACAA GTCAAAACAA AAAGGAAAGT TATGATTGCC TTCCGGTACA ATGCAGGACT
    ACACAATTAC CGTTTATGGT TTCAGAGTGT GAAAGAAAGG ATAGGGAATC CCTTGACATA
    GAGTGTTCTG CACTTATTAG AATGTCTGCA AATGAGTCAG TGGTCGATGA CTATTCAGAT
    TTTTCACTTA CTGAGGAAAA TGTTAACTTT GTAAATAATA ACAATATAAA TGAGCATACA
    GAAGAATGCA AAACCTGTTC CTGTCAGAGA ACAATCCCAT TTACTGGGAA AACCATCTGG
    AAATGTTCTT GTGCAAGGAC GTGTGTATGG GCTTTCCCAA GAAAAAGAAC TTCCTCTTTA
    CCAAGTACAG ATATACTGGG ATTTGACTCT GAATTGTCTT TTGCCCCTTA TTCCACCAAT
    ATAAAGTCTT TATTTCAGGC TAATGAAGCA GAGCCATTGC AGAGCACAAC TGATGACACT
    GACAGTGACG GGATATGTAA AAAACTAAAG TTAGATTTCA TTTCAGAAAA TGACGCTCTT
    ATTCTTAAAG ATATTGATGA TGACATTTTG TCTCACCCTC CAACAGAAAC TTTTGAACTA
    CCCAATACTG TAATTGAGAA GATATGTAAT ATAACTGAAA ATGTTTACTC ACTAGGTGAA
    GTTGAAGACA TGTATAAAAA TTTAGATGGT TGGCCATGCA GTTCAACTAT TATTCAAAAT
    ACTATAAATA GTCTTCCTCT CCCGCTGCCA AACAATGACA AAAGTGAGCA CTTTACCACT
    TCTGGCACTT ATTCTGAAAT AGAACCTACT GTAAAAACAG TTGAGGAGAT GGCCCTGAGC
    AGTTCTTTTG AAAACTCTGA TCCAAGTGTT TGTTCCAGAG ACAGCTCCAC AGAACCTGAA
    AACAGTTCAG AGACTGTGCT AAAAAGTACT CAATATGAAT CTCCAATACG TGATTGTTTA
    GACAAGCATA AAAAAAACAC TAATGTTGAG GAAGTGTTAT CCTTTTTTGA TTGTTTACAT
    GAAACTACAA TGGATAACGC CTGTCTAGCT GAGGAAAATT GCACCAGACA GATTAATAAT
    ACTGGTGGCC CATTAGATGA TTCACATTTG ACACTAAGCC ATGTAGAATC TCCAAAGAAA
    AGCAAGCTTG TGGAATACAA AAGTAATTTA GATGTGATAA AAGCTTATGA AGAAGATGTA
    CTGTTTTTAG ATGTTGTTCC AGATGATCCA GAGCTTTTTG GGACTCCTGA AGATCCTTAC
    ATAAGTCCAA TTTGTAATGC TGACAACAAA GAAAATAATA AACGGAATAA TCTGAGTGCA
    TTAATGTGTA ACCTTCAGGA ACAGGAGAGC GCCCATATCA ATGAAAGCAC AAATGATGCC
    AGCCCTGAGG AAAATGTAGA AGAGAGAAGT ACATATTTTG CAAAAGTAAT CACTGATCTA
    GAAGTTAATG ATGATCCTCT CATCATACAT AACACTTTGG AAAAGAACAG TGAAGATTTT
    TATGATGGTG GACAGTTTGA TTTTGAGGCG TTCAGAGATC TTGACGCAGA TAAAGAGATT
    AAATATGCTG AAAAGAATTC ATCTGAAAGC GGAAGCAGTT TTGAAGAGTC CTTGTCCAAG
    GATACTTCTC CGGGGTCCTC TCATTCCAAT AGCCGTCGTT GGAGAAGAGA TCCCTCACAT
    ACCTATAAGC CTATGCATTG GATGAATGAT TTCAGATACT CTGGAAAATG CATGGGATTG
    ACGGATGTGA TTACTTTGGA TCAGAAAGAA CAGAGGAATA TTGATAATCT CTTTTACGAT
    TTTACACCTA TACAGAAGAC CTTTTTGCCC ATTAGATACT GCAAATATTT TTTTAATACT
    TTCCGTGGAT GCATTAAACC AGATTGTATG TACCAGCACG TACCATTCCA AAGGGATGAA
    AAGGTGTGCA TGGAGGTGAT TCACAAACTT GTGAATGAAA ATCACACAAC ATTATTAAAA
    CGTGCTGTTT GGATATTTAC TGCCTACTAC AGAATGTATA TGCCTGGTGT TCATTATGAC
    TCAAATCTGC TGACCAAAAT GCTCCGTGCC TTGTATGTTC GTCAGATGTG GGGAGATGTA
    TTTCAGTTGT TGGAAACTGG TGCCAATGTC AAAATATTGC CCTCCTCTGA AATGCTTATC
    AGGTTATTTG AAAATATTGG CTCTACGGGA TTAACAGCAG CTGTACCTAG TTTAGTGGAT
    GTATTCTGCA AGCTTGTGGA GGCTGGAATG ATGATGAAGC CAGAGCAAAT CAACATGTTA
    ATAACAACAC TGAATAATCT CCAGGCAACA AAAAATTATA TCAGTGTCAT TTTAGACATA
    AAAACAAGAA TTGAAATGCA GCTTTCAGAA AAGAACTGGC TGTGTAACTT GGATATAGCA
    GTTGCAGAAG TTGGGCATTG TAAAGAAAAA AATGATTGGA AGAAACTAGG AACATTATAT
    CTTAATCTGC GTACAGGGTG TGAGAATGTG ACTGATCTTA AGAAGTTTTC TAATTGCATT
    GTGGGGGCGC TTCAGAAAGT GCCAAGGAAT GATAAATCTG AAATTCCATA TTGTGACTTT
    GCAGACACAG TGTACAAAGA CGCCCAACTA AGTGAAATTG ACAAAAACAT TTTGGGAAGG
    ATTGGAATAT CCATCATGTA TCACTATTAC AGAAATAAAC AATCGCAAAA GGGAAAGAGG
    GTGCTCAGAA AACTTCAGGA AATGCAGATT AATTTCACAG TCCTAAAAGG ACTCACAGGG
    GAAGAGAGTA AAGCTACGCG CTGTCAAGTT GTGAACACAG CTGTAGAGAT CTTCTTGAAC
    TGTGAATACT TAAATGGTGC ACTGGGGGTT CTACGAGAGT CAGAGTGGAT CATTAACACA
    CAAGTGTGGC CATGTGAAAG AATGGATGTG CTGAAACGAC ATAACCTGTT GTGTACAATT
    GCACAGAAAA CACTGAATAA AAACATGTTT GATGTGTGTC TTGAGGTCCT TCAGAATCTG
    CCAGGACTGC AGTGCTCATT AACGGATGTG GACGTCTCTC AGTACAGCTT GCTTTTCAAC
    AAACTTTTGT CATCCTGCAT AGAAAATAAT ACACTTGGTG TATCCTCAAC TGTGATAGAT
    TTTATGATTG CAAAAAAAAT ACCACTTGAC TTTTTCTTAC TGAGAGCACT AATCACATCC
    TTGGGACGAA GCTGTCTATG GCTCAGAGCA AGAGAACTTT ATAAATGCGC TGTTTTGTTG
    GGTTGCTACC CGCCCATGGA AGGAAATCTG TATCGTAAAG TGCTCTTCAT CCCCTCATTC
    CTTACTGAGA TAGAAATGCT GTTATCCATT GAAATTTTCA TGGTGTCAAA TGCCAGTAGT
    ATTCAGTCAC CAGGAGGCAG CCATCAGACT CTGCAAATCA TTCTCAAAAG ATGTGAAGAA
    GAGAGAGTTC CGAATAAAGA TTATCAAAGA GGTAAAGCTA GCTATCAAGA TGCAGTGGAC
    CGGCTGATTC AGGCCTCTAG GCTATCAACA CCAAGATTAT TCATAAAGCA CCTGACTGTC
    AATAATGCCA ATGAACAGGT TTATATCCTT GATTACGGCA GCTCTCTCAA ATGGTTGCAT
    GAAAACATGA AATGGGCTGG GAAAGTATGG CTTTTTCAGG GGAAAATATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33111
    Clone ID Related Accession (Same CDS sequence) XM_012964409.2 , NM_001374744.1
    Accession Version XM_012964409.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5691bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product testis- and ovary-specific PAZ domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGAGTATGT TGCACTCTAG AAAAATTAAG CTAGTTCAAC CTAACCGGTC TGAATCCAGC 
    TCATTTGCTA AGATCTGTGA TGAGGCTGAT ACTGAAAAAA GTAGTTCAGT TCTTATCAGT
    CCAGCTCTCT CTCTACAGTA TTCCTCAAAA GCCATTGATC AGGATAGGAG TGTAAAGAAT
    CTGTCAGGTA GAAACCCAAA GGCCATAAGT GGTGTGAATG AGCCTTGTGC AATCCAGCAA
    TGTGGAAATA TAGCGGTGTC TACAGAATCT GACTTTATTA GAATGAATGA CACAAGGAAG
    GCAAGACTAG CACTTTCAGA TCCAGTTCTT GCTTCTAATG ACTCTGGAGA AAGTATTAAT
    GTTTCAAACT TGCCGTCTTG TCAAGTTAAA AGAAGAACCA GAAGGAAGGC AACTGATCTG
    CGCGGTGCGG AGTCAATTGA AAAAGAAAAG TGTGGAAGTT TAGTAGTAAA TCAGGAAGCA
    GCAATTGTGA ATACTGATAC TACTGTTCCA CTAGATATTG AGAATGTTTA CAGTCCTACT
    CATATGCTAA ATCAACGGGG ATTGTTGAAA AAAATAGGAA AGAGGCCAAC AATTAAGAAG
    GAAGATGTGA GTAGTGTAGG AGATAGACAA AGATGTTTAA AACTAGCAGG AAAGCAAAGT
    GGAAAGAAAC TGCAATCTGA GCAAAGTGTT GTGTGTGGCA AGGGAAAGAC TCAGGAATGT
    GTACAAATAA TAGGGAAAAA AAGAGGAAGG AGGGCAAAAA CTGATCAGAG TAGTGAGTGT
    GGCATAAGTG AGATTCAGCA AAGAAAGAAA AGTAGAAAAA AGGTAAAAAC TGATAAGAGT
    GATGTGTGTT GTTCAGGAGA GACATTGAAA TATGTTAATA AAATTAAAAG AGGGAGGAAA
    CTAAAAACTG ATCATAAAGT TGTAAGTAGC AAAGGAGAAA CACAGGAATG TGTAAAATTA
    ACAGACATGA ATCAGAAACC AAAAACTGAA TGTTTTGCTG TGTGTGGCAC AGAAGAGACT
    CAGGCTTGTG TAAAACCACT GGGAAAGAAA AAAGGCAGGA TACTAGGTAA TAGTGTTGGT
    AAAAATAAAA TTAATGCTGG GTGTTATTCA GAATTAGATA AAATGCGTAC CGTATATGTT
    AGGGTACAGC GATTGTCATT GAATAAAATT CCCCAGAGTG CAGGAATCTC CACAGAACAT
    CTGAGGAGTG TGCTGCTTCT GAAGTCTGAG TATAGACAGG AATGTGGGGA AGCAAGAACA
    GAAGAGAGGA ATGATGCTAC AGATGTGACC TTCTTAAAAA AGAATCTCAG GAGTCCTAGA
    CAGCAGAAAT GCTGTGGATT CCCTTGTGTT TCAGAGCATA AGAGGAAAAA GGAGAGTGCA
    CAGCAGAAAA CTTTATGTTC TACACACAAC TGTTATTTCA CTGTTACAGA TCCAGCACAC
    ACGGTAAATG GAATAACGTG CATGCAAAAT CCATTGCTTG AAACAGCAAA CAATAGAAAA
    AGTAAATGTT CATATGGTGG TTCCCAGTCT TTTCTAAATC CTGTAGTGAA ATTGCATAAC
    TGCAGCAACA TGAAGAGGCT TATACGTTGC TCAAATCTGA GGGTAAGCAG TTCCAAAACT
    GAGAATACAT GTCCTGCAGC AACAGTAAAA GAACTTGATC CTCAAAAACA ATTACCTTGT
    TCTGTTAACA CTGTAAATTC AGTTATCAGT ACAGATTCTA CTGTTGGAGG TAATCATTCC
    AAAGAACTGG AATCTATTGC ACATGGCTTG GCAAATAGGA AAATTAAAAG AATATTAAAA
    GTAAAGTGGG ACTTTGGAAA AAAAATAAGG AATTCTGCAA CATGCTCTAC AGAGCGTACT
    CAAGAAGACT CTGCAATATC TGTCAAACAG AAAACAGGAG ACCTAAAATA TAGTGGTAAT
    CCTACTGTCA ACACAGACTC ATCAGATCCT TGCATTGGTA CTTTGGAAAA GATTATTGAC
    GAGATGAACA ATAATAAAAG TGACTCTGTA AATTTTAACT TGAAGGATGC CCTTTGTGAT
    CAAAGCACAA GTCAAAACAA AAAGGAAAGT TATGATTGCC TTCCGGTACA ATGCAGGACT
    ACACAATTAC CGTTTATGGT TTCAGAGTGT GAAAGAAAGG ATAGGGAATC CCTTGACATA
    GAGTGTTCTG CACTTATTAG AATGTCTGCA AATGAGTCAG TGGTCGATGA CTATTCAGAT
    TTTTCACTTA CTGAGGAAAA TGTTAACTTT GTAAATAATA ACAATATAAA TGAGCATACA
    GAAGAATGCA AAACCTGTTC CTGTCAGAGA ACAATCCCAT TTACTGGGAA AACCATCTGG
    AAATGTTCTT GTGCAAGGAC GTGTGTATGG GCTTTCCCAA GAAAAAGAAC TTCCTCTTTA
    CCAAGTACAG ATATACTGGG ATTTGACTCT GAATTGTCTT TTGCCCCTTA TTCCACCAAT
    ATAAAGTCTT TATTTCAGGC TAATGAAGCA GAGCCATTGC AGAGCACAAC TGATGACACT
    GACAGTGACG GGATATGTAA AAAACTAAAG TTAGATTTCA TTTCAGAAAA TGACGCTCTT
    ATTCTTAAAG ATATTGATGA TGACATTTTG TCTCACCCTC CAACAGAAAC TTTTGAACTA
    CCCAATACTG TAATTGAGAA GATATGTAAT ATAACTGAAA ATGTTTACTC ACTAGGTGAA
    GTTGAAGACA TGTATAAAAA TTTAGATGGT TGGCCATGCA GTTCAACTAT TATTCAAAAT
    ACTATAAATA GTCTTCCTCT CCCGCTGCCA AACAATGACA AAAGTGAGCA CTTTACCACT
    TCTGGCACTT ATTCTGAAAT AGAACCTACT GTAAAAACAG TTGAGGAGAT GGCCCTGAGC
    AGTTCTTTTG AAAACTCTGA TCCAAGTGTT TGTTCCAGAG ACAGCTCCAC AGAACCTGAA
    AACAGTTCAG AGACTGTGCT AAAAAGTACT CAATATGAAT CTCCAATACG TGATTGTTTA
    GACAAGCATA AAAAAAACAC TAATGTTGAG GAAGTGTTAT CCTTTTTTGA TTGTTTACAT
    GAAACTACAA TGGATAACGC CTGTCTAGCT GAGGAAAATT GCACCAGACA GATTAATAAT
    ACTGGTGGCC CATTAGATGA TTCACATTTG ACACTAAGCC ATGTAGAATC TCCAAAGAAA
    AGCAAGCTTG TGGAATACAA AAGTAATTTA GATGTGATAA AAGCTTATGA AGAAGATGTA
    CTGTTTTTAG ATGTTGTTCC AGATGATCCA GAGCTTTTTG GGACTCCTGA AGATCCTTAC
    ATAAGTCCAA TTTGTAATGC TGACAACAAA GAAAATAATA AACGGAATAA TCTGAGTGCA
    TTAATGTGTA ACCTTCAGGA ACAGGAGAGC GCCCATATCA ATGAAAGCAC AAATGATGCC
    AGCCCTGAGG AAAATGTAGA AGAGAGAAGT ACATATTTTG CAAAAGTAAT CACTGATCTA
    GAAGTTAATG ATGATCCTCT CATCATACAT AACACTTTGG AAAAGAACAG TGAAGATTTT
    TATGATGGTG GACAGTTTGA TTTTGAGGCG TTCAGAGATC TTGACGCAGA TAAAGAGATT
    AAATATGCTG AAAAGAATTC ATCTGAAAGC GGAAGCAGTT TTGAAGAGTC CTTGTCCAAG
    GATACTTCTC CGGGGTCCTC TCATTCCAAT AGCCGTCGTT GGAGAAGAGA TCCCTCACAT
    ACCTATAAGC CTATGCATTG GATGAATGAT TTCAGATACT CTGGAAAATG CATGGGATTG
    ACGGATGTGA TTACTTTGGA TCAGAAAGAA CAGAGGAATA TTGATAATCT CTTTTACGAT
    TTTACACCTA TACAGAAGAC CTTTTTGCCC ATTAGATACT GCAAATATTT TTTTAATACT
    TTCCGTGGAT GCATTAAACC AGATTGTATG TACCAGCACG TACCATTCCA AAGGGATGAA
    AAGGTGTGCA TGGAGGTGAT TCACAAACTT GTGAATGAAA ATCACACAAC ATTATTAAAA
    CGTGCTGTTT GGATATTTAC TGCCTACTAC AGAATGTATA TGCCTGGTGT TCATTATGAC
    TCAAATCTGC TGACCAAAAT GCTCCGTGCC TTGTATGTTC GTCAGATGTG GGGAGATGTA
    TTTCAGTTGT TGGAAACTGG TGCCAATGTC AAAATATTGC CCTCCTCTGA AATGCTTATC
    AGGTTATTTG AAAATATTGG CTCTACGGGA TTAACAGCAG CTGTACCTAG TTTAGTGGAT
    GTATTCTGCA AGCTTGTGGA GGCTGGAATG ATGATGAAGC CAGAGCAAAT CAACATGTTA
    ATAACAACAC TGAATAATCT CCAGGCAACA AAAAATTATA TCAGTGTCAT TTTAGACATA
    AAAACAAGAA TTGAAATGCA GCTTTCAGAA AAGAACTGGC TGTGTAACTT GGATATAGCA
    GTTGCAGAAG TTGGGCATTG TAAAGAAAAA AATGATTGGA AGAAACTAGG AACATTATAT
    CTTAATCTGC GTACAGGGTG TGAGAATGTG ACTGATCTTA AGAAGTTTTC TAATTGCATT
    GTGGGGGCGC TTCAGAAAGT GCCAAGGAAT GATAAATCTG AAATTCCATA TTGTGACTTT
    GCAGACACAG TGTACAAAGA CGCCCAACTA AGTGAAATTG ACAAAAACAT TTTGGGAAGG
    ATTGGAATAT CCATCATGTA TCACTATTAC AGAAATAAAC AATCGCAAAA GGGAAAGAGG
    GTGCTCAGAA AACTTCAGGA AATGCAGATT AATTTCACAG TCCTAAAAGG ACTCACAGGG
    GAAGAGAGTA AAGCTACGCG CTGTCAAGTT GTGAACACAG CTGTAGAGAT CTTCTTGAAC
    TGTGAATACT TAAATGGTGC ACTGGGGGTT CTACGAGAGT CAGAGTGGAT CATTAACACA
    CAAGTGTGGC CATGTGAAAG AATGGATGTG CTGAAACGAC ATAACCTGTT GTGTACAATT
    GCACAGAAAA CACTGAATAA AAACATGTTT GATGTGTGTC TTGAGGTCCT TCAGAATCTG
    CCAGGACTGC AGTGCTCATT AACGGATGTG GACGTCTCTC AGTACAGCTT GCTTTTCAAC
    AAACTTTTGT CATCCTGCAT AGAAAATAAT ACACTTGGTG TATCCTCAAC TGTGATAGAT
    TTTATGATTG CAAAAAAAAT ACCACTTGAC TTTTTCTTAC TGAGAGCACT AATCACATCC
    TTGGGACGAA GCTGTCTATG GCTCAGAGCA AGAGAACTTT ATAAATGCGC TGTTTTGTTG
    GGTTGCTACC CGCCCATGGA AGGAAATCTG TATCGTAAAG TGCTCTTCAT CCCCTCATTC
    CTTACTGAGA TAGAAATGCT GTTATCCATT GAAATTTTCA TGGTGTCAAA TGCCAGTAGT
    ATTCAGTCAC CAGGAGGCAG CCATCAGACT CTGCAAATCA TTCTCAAAAG ATGTGAAGAA
    GAGAGAGTTC CGAATAAAGA TTATCAAAGA GGTAAAGCTA GCTATCAAGA TGCAGTGGAC
    CGGCTGATTC AGGCCTCTAG GCTATCAACA CCAAGATTAT TCATAAAGCA CCTGACTGTC
    AATAATGCCA ATGAACAGGT TTATATCCTT GATTACGGCA GCTCTCTCAA ATGGTTGCAT
    GAAAACATGA AATGGGCTGG GAAAGTATGG CTTTTTCAGG GGAAAATATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819863.1
    CDS777..6467
    Translation

    Target ORF information:

    RefSeq Version XM_012964409.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964409.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGAGTATGT TGCACTCTAG AAAAATTAAG CTAGTTCAAC CTAACCGGTC TGAATCCAGC 
    TCATTTGCTA AGATCTGTGA TGAGGCTGAT ACTGAAAAAA GTAGTTCAGT TCTTATCAGT
    CCAGCTCTCT CTCTACAGTA TTCCTCAAAA GCCATTGATC AGGATAGGAG TGTAAAGAAT
    CTGTCAGGTA GAAACCCAAA GGCCATAAGT GGTGTGAATG AGCCTTGTGC AATCCAGCAA
    TGTGGAAATA TAGCGGTGTC TACAGAATCT GACTTTATTA GAATGAATGA CACAAGGAAG
    GCAAGACTAG CACTTTCAGA TCCAGTTCTT GCTTCTAATG ACTCTGGAGA AAGTATTAAT
    GTTTCAAACT TGCCGTCTTG TCAAGTTAAA AGAAGAACCA GAAGGAAGGC AACTGATCTG
    CGCGGTGCGG AGTCAATTGA AAAAGAAAAG TGTGGAAGTT TAGTAGTAAA TCAGGAAGCA
    GCAATTGTGA ATACTGATAC TACTGTTCCA CTAGATATTG AGAATGTTTA CAGTCCTACT
    CATATGCTAA ATCAACGGGG ATTGTTGAAA AAAATAGGAA AGAGGCCAAC AATTAAGAAG
    GAAGATGTGA GTAGTGTAGG AGATAGACAA AGATGTTTAA AACTAGCAGG AAAGCAAAGT
    GGAAAGAAAC TGCAATCTGA GCAAAGTGTT GTGTGTGGCA AGGGAAAGAC TCAGGAATGT
    GTACAAATAA TAGGGAAAAA AAGAGGAAGG AGGGCAAAAA CTGATCAGAG TAGTGAGTGT
    GGCATAAGTG AGATTCAGCA AAGAAAGAAA AGTAGAAAAA AGGTAAAAAC TGATAAGAGT
    GATGTGTGTT GTTCAGGAGA GACATTGAAA TATGTTAATA AAATTAAAAG AGGGAGGAAA
    CTAAAAACTG ATCATAAAGT TGTAAGTAGC AAAGGAGAAA CACAGGAATG TGTAAAATTA
    ACAGACATGA ATCAGAAACC AAAAACTGAA TGTTTTGCTG TGTGTGGCAC AGAAGAGACT
    CAGGCTTGTG TAAAACCACT GGGAAAGAAA AAAGGCAGGA TACTAGGTAA TAGTGTTGGT
    AAAAATAAAA TTAATGCTGG GTGTTATTCA GAATTAGATA AAATGCGTAC CGTATATGTT
    AGGGTACAGC GATTGTCATT GAATAAAATT CCCCAGAGTG CAGGAATCTC CACAGAACAT
    CTGAGGAGTG TGCTGCTTCT GAAGTCTGAG TATAGACAGG AATGTGGGGA AGCAAGAACA
    GAAGAGAGGA ATGATGCTAC AGATGTGACC TTCTTAAAAA AGAATCTCAG GAGTCCTAGA
    CAGCAGAAAT GCTGTGGATT CCCTTGTGTT TCAGAGCATA AGAGGAAAAA GGAGAGTGCA
    CAGCAGAAAA CTTTATGTTC TACACACAAC TGTTATTTCA CTGTTACAGA TCCAGCACAC
    ACGGTAAATG GAATAACGTG CATGCAAAAT CCATTGCTTG AAACAGCAAA CAATAGAAAA
    AGTAAATGTT CATATGGTGG TTCCCAGTCT TTTCTAAATC CTGTAGTGAA ATTGCATAAC
    TGCAGCAACA TGAAGAGGCT TATACGTTGC TCAAATCTGA GGGTAAGCAG TTCCAAAACT
    GAGAATACAT GTCCTGCAGC AACAGTAAAA GAACTTGATC CTCAAAAACA ATTACCTTGT
    TCTGTTAACA CTGTAAATTC AGTTATCAGT ACAGATTCTA CTGTTGGAGG TAATCATTCC
    AAAGAACTGG AATCTATTGC ACATGGCTTG GCAAATAGGA AAATTAAAAG AATATTAAAA
    GTAAAGTGGG ACTTTGGAAA AAAAATAAGG AATTCTGCAA CATGCTCTAC AGAGCGTACT
    CAAGAAGACT CTGCAATATC TGTCAAACAG AAAACAGGAG ACCTAAAATA TAGTGGTAAT
    CCTACTGTCA ACACAGACTC ATCAGATCCT TGCATTGGTA CTTTGGAAAA GATTATTGAC
    GAGATGAACA ATAATAAAAG TGACTCTGTA AATTTTAACT TGAAGGATGC CCTTTGTGAT
    CAAAGCACAA GTCAAAACAA AAAGGAAAGT TATGATTGCC TTCCGGTACA ATGCAGGACT
    ACACAATTAC CGTTTATGGT TTCAGAGTGT GAAAGAAAGG ATAGGGAATC CCTTGACATA
    GAGTGTTCTG CACTTATTAG AATGTCTGCA AATGAGTCAG TGGTCGATGA CTATTCAGAT
    TTTTCACTTA CTGAGGAAAA TGTTAACTTT GTAAATAATA ACAATATAAA TGAGCATACA
    GAAGAATGCA AAACCTGTTC CTGTCAGAGA ACAATCCCAT TTACTGGGAA AACCATCTGG
    AAATGTTCTT GTGCAAGGAC GTGTGTATGG GCTTTCCCAA GAAAAAGAAC TTCCTCTTTA
    CCAAGTACAG ATATACTGGG ATTTGACTCT GAATTGTCTT TTGCCCCTTA TTCCACCAAT
    ATAAAGTCTT TATTTCAGGC TAATGAAGCA GAGCCATTGC AGAGCACAAC TGATGACACT
    GACAGTGACG GGATATGTAA AAAACTAAAG TTAGATTTCA TTTCAGAAAA TGACGCTCTT
    ATTCTTAAAG ATATTGATGA TGACATTTTG TCTCACCCTC CAACAGAAAC TTTTGAACTA
    CCCAATACTG TAATTGAGAA GATATGTAAT ATAACTGAAA ATGTTTACTC ACTAGGTGAA
    GTTGAAGACA TGTATAAAAA TTTAGATGGT TGGCCATGCA GTTCAACTAT TATTCAAAAT
    ACTATAAATA GTCTTCCTCT CCCGCTGCCA AACAATGACA AAAGTGAGCA CTTTACCACT
    TCTGGCACTT ATTCTGAAAT AGAACCTACT GTAAAAACAG TTGAGGAGAT GGCCCTGAGC
    AGTTCTTTTG AAAACTCTGA TCCAAGTGTT TGTTCCAGAG ACAGCTCCAC AGAACCTGAA
    AACAGTTCAG AGACTGTGCT AAAAAGTACT CAATATGAAT CTCCAATACG TGATTGTTTA
    GACAAGCATA AAAAAAACAC TAATGTTGAG GAAGTGTTAT CCTTTTTTGA TTGTTTACAT
    GAAACTACAA TGGATAACGC CTGTCTAGCT GAGGAAAATT GCACCAGACA GATTAATAAT
    ACTGGTGGCC CATTAGATGA TTCACATTTG ACACTAAGCC ATGTAGAATC TCCAAAGAAA
    AGCAAGCTTG TGGAATACAA AAGTAATTTA GATGTGATAA AAGCTTATGA AGAAGATGTA
    CTGTTTTTAG ATGTTGTTCC AGATGATCCA GAGCTTTTTG GGACTCCTGA AGATCCTTAC
    ATAAGTCCAA TTTGTAATGC TGACAACAAA GAAAATAATA AACGGAATAA TCTGAGTGCA
    TTAATGTGTA ACCTTCAGGA ACAGGAGAGC GCCCATATCA ATGAAAGCAC AAATGATGCC
    AGCCCTGAGG AAAATGTAGA AGAGAGAAGT ACATATTTTG CAAAAGTAAT CACTGATCTA
    GAAGTTAATG ATGATCCTCT CATCATACAT AACACTTTGG AAAAGAACAG TGAAGATTTT
    TATGATGGTG GACAGTTTGA TTTTGAGGCG TTCAGAGATC TTGACGCAGA TAAAGAGATT
    AAATATGCTG AAAAGAATTC ATCTGAAAGC GGAAGCAGTT TTGAAGAGTC CTTGTCCAAG
    GATACTTCTC CGGGGTCCTC TCATTCCAAT AGCCGTCGTT GGAGAAGAGA TCCCTCACAT
    ACCTATAAGC CTATGCATTG GATGAATGAT TTCAGATACT CTGGAAAATG CATGGGATTG
    ACGGATGTGA TTACTTTGGA TCAGAAAGAA CAGAGGAATA TTGATAATCT CTTTTACGAT
    TTTACACCTA TACAGAAGAC CTTTTTGCCC ATTAGATACT GCAAATATTT TTTTAATACT
    TTCCGTGGAT GCATTAAACC AGATTGTATG TACCAGCACG TACCATTCCA AAGGGATGAA
    AAGGTGTGCA TGGAGGTGAT TCACAAACTT GTGAATGAAA ATCACACAAC ATTATTAAAA
    CGTGCTGTTT GGATATTTAC TGCCTACTAC AGAATGTATA TGCCTGGTGT TCATTATGAC
    TCAAATCTGC TGACCAAAAT GCTCCGTGCC TTGTATGTTC GTCAGATGTG GGGAGATGTA
    TTTCAGTTGT TGGAAACTGG TGCCAATGTC AAAATATTGC CCTCCTCTGA AATGCTTATC
    AGGTTATTTG AAAATATTGG CTCTACGGGA TTAACAGCAG CTGTACCTAG TTTAGTGGAT
    GTATTCTGCA AGCTTGTGGA GGCTGGAATG ATGATGAAGC CAGAGCAAAT CAACATGTTA
    ATAACAACAC TGAATAATCT CCAGGCAACA AAAAATTATA TCAGTGTCAT TTTAGACATA
    AAAACAAGAA TTGAAATGCA GCTTTCAGAA AAGAACTGGC TGTGTAACTT GGATATAGCA
    GTTGCAGAAG TTGGGCATTG TAAAGAAAAA AATGATTGGA AGAAACTAGG AACATTATAT
    CTTAATCTGC GTACAGGGTG TGAGAATGTG ACTGATCTTA AGAAGTTTTC TAATTGCATT
    GTGGGGGCGC TTCAGAAAGT GCCAAGGAAT GATAAATCTG AAATTCCATA TTGTGACTTT
    GCAGACACAG TGTACAAAGA CGCCCAACTA AGTGAAATTG ACAAAAACAT TTTGGGAAGG
    ATTGGAATAT CCATCATGTA TCACTATTAC AGAAATAAAC AATCGCAAAA GGGAAAGAGG
    GTGCTCAGAA AACTTCAGGA AATGCAGATT AATTTCACAG TCCTAAAAGG ACTCACAGGG
    GAAGAGAGTA AAGCTACGCG CTGTCAAGTT GTGAACACAG CTGTAGAGAT CTTCTTGAAC
    TGTGAATACT TAAATGGTGC ACTGGGGGTT CTACGAGAGT CAGAGTGGAT CATTAACACA
    CAAGTGTGGC CATGTGAAAG AATGGATGTG CTGAAACGAC ATAACCTGTT GTGTACAATT
    GCACAGAAAA CACTGAATAA AAACATGTTT GATGTGTGTC TTGAGGTCCT TCAGAATCTG
    CCAGGACTGC AGTGCTCATT AACGGATGTG GACGTCTCTC AGTACAGCTT GCTTTTCAAC
    AAACTTTTGT CATCCTGCAT AGAAAATAAT ACACTTGGTG TATCCTCAAC TGTGATAGAT
    TTTATGATTG CAAAAAAAAT ACCACTTGAC TTTTTCTTAC TGAGAGCACT AATCACATCC
    TTGGGACGAA GCTGTCTATG GCTCAGAGCA AGAGAACTTT ATAAATGCGC TGTTTTGTTG
    GGTTGCTACC CGCCCATGGA AGGAAATCTG TATCGTAAAG TGCTCTTCAT CCCCTCATTC
    CTTACTGAGA TAGAAATGCT GTTATCCATT GAAATTTTCA TGGTGTCAAA TGCCAGTAGT
    ATTCAGTCAC CAGGAGGCAG CCATCAGACT CTGCAAATCA TTCTCAAAAG ATGTGAAGAA
    GAGAGAGTTC CGAATAAAGA TTATCAAAGA GGTAAAGCTA GCTATCAAGA TGCAGTGGAC
    CGGCTGATTC AGGCCTCTAG GCTATCAACA CCAAGATTAT TCATAAAGCA CCTGACTGTC
    AATAATGCCA ATGAACAGGT TTATATCCTT GATTACGGCA GCTCTCTCAA ATGGTTGCAT
    GAAAACATGA AATGGGCTGG GAAAGTATGG CTTTTTCAGG GGAAAATATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33112
    Clone ID Related Accession (Same CDS sequence) XM_012964412.2 , XM_012964413.2 , XM_012964411.2
    Accession Version XM_012964412.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product testis- and ovary-specific PAZ domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCAAAATC CATTGCTTGA AACAGCAAAC AATAGAAAAA GTAAATGTTC ATATGGTGGT 
    TCCCAGTCTT TTCTAAATCC TGTAGTGAAA TTGCATAACT GCAGCAACAT GAAGAGGCTT
    ATACGTTGCT CAAATCTGAG GGTAAGCAGT TCCAAAACTG AGAATACATG TCCTGCAGCA
    ACAGTAAAAG AACTTGATCC TCAAAAACAA TTACCTTGTT CTGTTAACAC TGTAAATTCA
    GTTATCAGTA CAGATTCTAC TGTTGGAGGT AATCATTCCA AAGAACTGGA ATCTATTGCA
    CATGGCTTGG CAAATAGGAA AATTAAAAGA ATATTAAAAG TAAAGTGGGA CTTTGGAAAA
    AAAATAAGGA ATTCTGCAAC ATGCTCTACA GAGCGTACTC AAGAAGACTC TGCAATATCT
    GTCAAACAGA AAACAGGAGA CCTAAAATAT AGTGGTAATC CTACTGTCAA CACAGACTCA
    TCAGATCCTT GCATTGGTAC TTTGGAAAAG ATTATTGACG AGATGAACAA TAATAAAAGT
    GACTCTGTAA ATTTTAACTT GAAGGATGCC CTTTGTGATC AAAGCACAAG TCAAAACAAA
    AAGGAAAGTT ATGATTGCCT TCCGGTACAA TGCAGGACTA CACAATTACC GTTTATGGTT
    TCAGAGTGTG AAAGAAAGGA TAGGGAATCC CTTGACATAG AGTGTTCTGC ACTTATTAGA
    ATGTCTGCAA ATGAGTCAGT GGTCGATGAC TATTCAGATT TTTCACTTAC TGAGGAAAAT
    GTTAACTTTG TAAATAATAA CAATATAAAT GAGCATACAG AAGAATGCAA AACCTGTTCC
    TGTCAGAGAA CAATCCCATT TACTGGGAAA ACCATCTGGA AATGTTCTTG TGCAAGGACG
    TGTGTATGGG CTTTCCCAAG AAAAAGAACT TCCTCTTTAC CAAGTACAGA TATACTGGGA
    TTTGACTCTG AATTGTCTTT TGCCCCTTAT TCCACCAATA TAAAGTCTTT ATTTCAGGCT
    AATGAAGCAG AGCCATTGCA GAGCACAACT GATGACACTG ACAGTGACGG GATATGTAAA
    AAACTAAAGT TAGATTTCAT TTCAGAAAAT GACGCTCTTA TTCTTAAAGA TATTGATGAT
    GACATTTTGT CTCACCCTCC AACAGAAACT TTTGAACTAC CCAATACTGT AATTGAGAAG
    ATATGTAATA TAACTGAAAA TGTTTACTCA CTAGGTGAAG TTGAAGACAT GTATAAAAAT
    TTAGATGGTT GGCCATGCAG TTCAACTATT ATTCAAAATA CTATAAATAG TCTTCCTCTC
    CCGCTGCCAA ACAATGACAA AAGTGAGCAC TTTACCACTT CTGGCACTTA TTCTGAAATA
    GAACCTACTG TAAAAACAGT TGAGGAGATG GCCCTGAGCA GTTCTTTTGA AAACTCTGAT
    CCAAGTGTTT GTTCCAGAGA CAGCTCCACA GAACCTGAAA ACAGTTCAGA GACTGTGCTA
    AAAAGTACTC AATATGAATC TCCAATACGT GATTGTTTAG ACAAGCATAA AAAAAACACT
    AATGTTGAGG AAGTGTTATC CTTTTTTGAT TGTTTACATG AAACTACAAT GGATAACGCC
    TGTCTAGCTG AGGAAAATTG CACCAGACAG ATTAATAATA CTGGTGGCCC ATTAGATGAT
    TCACATTTGA CACTAAGCCA TGTAGAATCT CCAAAGAAAA GCAAGCTTGT GGAATACAAA
    AGTAATTTAG ATGTGATAAA AGCTTATGAA GAAGATGTAC TGTTTTTAGA TGTTGTTCCA
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819866.1
    CDS290..4519
    Translation

    Target ORF information:

    RefSeq Version XM_012964412.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964412.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCAAAATC CATTGCTTGA AACAGCAAAC AATAGAAAAA GTAAATGTTC ATATGGTGGT 
    TCCCAGTCTT TTCTAAATCC TGTAGTGAAA TTGCATAACT GCAGCAACAT GAAGAGGCTT
    ATACGTTGCT CAAATCTGAG GGTAAGCAGT TCCAAAACTG AGAATACATG TCCTGCAGCA
    ACAGTAAAAG AACTTGATCC TCAAAAACAA TTACCTTGTT CTGTTAACAC TGTAAATTCA
    GTTATCAGTA CAGATTCTAC TGTTGGAGGT AATCATTCCA AAGAACTGGA ATCTATTGCA
    CATGGCTTGG CAAATAGGAA AATTAAAAGA ATATTAAAAG TAAAGTGGGA CTTTGGAAAA
    AAAATAAGGA ATTCTGCAAC ATGCTCTACA GAGCGTACTC AAGAAGACTC TGCAATATCT
    GTCAAACAGA AAACAGGAGA CCTAAAATAT AGTGGTAATC CTACTGTCAA CACAGACTCA
    TCAGATCCTT GCATTGGTAC TTTGGAAAAG ATTATTGACG AGATGAACAA TAATAAAAGT
    GACTCTGTAA ATTTTAACTT GAAGGATGCC CTTTGTGATC AAAGCACAAG TCAAAACAAA
    AAGGAAAGTT ATGATTGCCT TCCGGTACAA TGCAGGACTA CACAATTACC GTTTATGGTT
    TCAGAGTGTG AAAGAAAGGA TAGGGAATCC CTTGACATAG AGTGTTCTGC ACTTATTAGA
    ATGTCTGCAA ATGAGTCAGT GGTCGATGAC TATTCAGATT TTTCACTTAC TGAGGAAAAT
    GTTAACTTTG TAAATAATAA CAATATAAAT GAGCATACAG AAGAATGCAA AACCTGTTCC
    TGTCAGAGAA CAATCCCATT TACTGGGAAA ACCATCTGGA AATGTTCTTG TGCAAGGACG
    TGTGTATGGG CTTTCCCAAG AAAAAGAACT TCCTCTTTAC CAAGTACAGA TATACTGGGA
    TTTGACTCTG AATTGTCTTT TGCCCCTTAT TCCACCAATA TAAAGTCTTT ATTTCAGGCT
    AATGAAGCAG AGCCATTGCA GAGCACAACT GATGACACTG ACAGTGACGG GATATGTAAA
    AAACTAAAGT TAGATTTCAT TTCAGAAAAT GACGCTCTTA TTCTTAAAGA TATTGATGAT
    GACATTTTGT CTCACCCTCC AACAGAAACT TTTGAACTAC CCAATACTGT AATTGAGAAG
    ATATGTAATA TAACTGAAAA TGTTTACTCA CTAGGTGAAG TTGAAGACAT GTATAAAAAT
    TTAGATGGTT GGCCATGCAG TTCAACTATT ATTCAAAATA CTATAAATAG TCTTCCTCTC
    CCGCTGCCAA ACAATGACAA AAGTGAGCAC TTTACCACTT CTGGCACTTA TTCTGAAATA
    GAACCTACTG TAAAAACAGT TGAGGAGATG GCCCTGAGCA GTTCTTTTGA AAACTCTGAT
    CCAAGTGTTT GTTCCAGAGA CAGCTCCACA GAACCTGAAA ACAGTTCAGA GACTGTGCTA
    AAAAGTACTC AATATGAATC TCCAATACGT GATTGTTTAG ACAAGCATAA AAAAAACACT
    AATGTTGAGG AAGTGTTATC CTTTTTTGAT TGTTTACATG AAACTACAAT GGATAACGCC
    TGTCTAGCTG AGGAAAATTG CACCAGACAG ATTAATAATA CTGGTGGCCC ATTAGATGAT
    TCACATTTGA CACTAAGCCA TGTAGAATCT CCAAAGAAAA GCAAGCTTGT GGAATACAAA
    AGTAATTTAG ATGTGATAAA AGCTTATGAA GAAGATGTAC TGTTTTTAGA TGTTGTTCCA
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33112
    Clone ID Related Accession (Same CDS sequence) XM_012964412.2 , XM_012964413.2 , XM_012964411.2
    Accession Version XM_012964413.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product testis- and ovary-specific PAZ domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCAAAATC CATTGCTTGA AACAGCAAAC AATAGAAAAA GTAAATGTTC ATATGGTGGT 
    TCCCAGTCTT TTCTAAATCC TGTAGTGAAA TTGCATAACT GCAGCAACAT GAAGAGGCTT
    ATACGTTGCT CAAATCTGAG GGTAAGCAGT TCCAAAACTG AGAATACATG TCCTGCAGCA
    ACAGTAAAAG AACTTGATCC TCAAAAACAA TTACCTTGTT CTGTTAACAC TGTAAATTCA
    GTTATCAGTA CAGATTCTAC TGTTGGAGGT AATCATTCCA AAGAACTGGA ATCTATTGCA
    CATGGCTTGG CAAATAGGAA AATTAAAAGA ATATTAAAAG TAAAGTGGGA CTTTGGAAAA
    AAAATAAGGA ATTCTGCAAC ATGCTCTACA GAGCGTACTC AAGAAGACTC TGCAATATCT
    GTCAAACAGA AAACAGGAGA CCTAAAATAT AGTGGTAATC CTACTGTCAA CACAGACTCA
    TCAGATCCTT GCATTGGTAC TTTGGAAAAG ATTATTGACG AGATGAACAA TAATAAAAGT
    GACTCTGTAA ATTTTAACTT GAAGGATGCC CTTTGTGATC AAAGCACAAG TCAAAACAAA
    AAGGAAAGTT ATGATTGCCT TCCGGTACAA TGCAGGACTA CACAATTACC GTTTATGGTT
    TCAGAGTGTG AAAGAAAGGA TAGGGAATCC CTTGACATAG AGTGTTCTGC ACTTATTAGA
    ATGTCTGCAA ATGAGTCAGT GGTCGATGAC TATTCAGATT TTTCACTTAC TGAGGAAAAT
    GTTAACTTTG TAAATAATAA CAATATAAAT GAGCATACAG AAGAATGCAA AACCTGTTCC
    TGTCAGAGAA CAATCCCATT TACTGGGAAA ACCATCTGGA AATGTTCTTG TGCAAGGACG
    TGTGTATGGG CTTTCCCAAG AAAAAGAACT TCCTCTTTAC CAAGTACAGA TATACTGGGA
    TTTGACTCTG AATTGTCTTT TGCCCCTTAT TCCACCAATA TAAAGTCTTT ATTTCAGGCT
    AATGAAGCAG AGCCATTGCA GAGCACAACT GATGACACTG ACAGTGACGG GATATGTAAA
    AAACTAAAGT TAGATTTCAT TTCAGAAAAT GACGCTCTTA TTCTTAAAGA TATTGATGAT
    GACATTTTGT CTCACCCTCC AACAGAAACT TTTGAACTAC CCAATACTGT AATTGAGAAG
    ATATGTAATA TAACTGAAAA TGTTTACTCA CTAGGTGAAG TTGAAGACAT GTATAAAAAT
    TTAGATGGTT GGCCATGCAG TTCAACTATT ATTCAAAATA CTATAAATAG TCTTCCTCTC
    CCGCTGCCAA ACAATGACAA AAGTGAGCAC TTTACCACTT CTGGCACTTA TTCTGAAATA
    GAACCTACTG TAAAAACAGT TGAGGAGATG GCCCTGAGCA GTTCTTTTGA AAACTCTGAT
    CCAAGTGTTT GTTCCAGAGA CAGCTCCACA GAACCTGAAA ACAGTTCAGA GACTGTGCTA
    AAAAGTACTC AATATGAATC TCCAATACGT GATTGTTTAG ACAAGCATAA AAAAAACACT
    AATGTTGAGG AAGTGTTATC CTTTTTTGAT TGTTTACATG AAACTACAAT GGATAACGCC
    TGTCTAGCTG AGGAAAATTG CACCAGACAG ATTAATAATA CTGGTGGCCC ATTAGATGAT
    TCACATTTGA CACTAAGCCA TGTAGAATCT CCAAAGAAAA GCAAGCTTGT GGAATACAAA
    AGTAATTTAG ATGTGATAAA AGCTTATGAA GAAGATGTAC TGTTTTTAGA TGTTGTTCCA
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819867.1
    CDS219..4448
    Translation

    Target ORF information:

    RefSeq Version XM_012964413.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964413.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCAAAATC CATTGCTTGA AACAGCAAAC AATAGAAAAA GTAAATGTTC ATATGGTGGT 
    TCCCAGTCTT TTCTAAATCC TGTAGTGAAA TTGCATAACT GCAGCAACAT GAAGAGGCTT
    ATACGTTGCT CAAATCTGAG GGTAAGCAGT TCCAAAACTG AGAATACATG TCCTGCAGCA
    ACAGTAAAAG AACTTGATCC TCAAAAACAA TTACCTTGTT CTGTTAACAC TGTAAATTCA
    GTTATCAGTA CAGATTCTAC TGTTGGAGGT AATCATTCCA AAGAACTGGA ATCTATTGCA
    CATGGCTTGG CAAATAGGAA AATTAAAAGA ATATTAAAAG TAAAGTGGGA CTTTGGAAAA
    AAAATAAGGA ATTCTGCAAC ATGCTCTACA GAGCGTACTC AAGAAGACTC TGCAATATCT
    GTCAAACAGA AAACAGGAGA CCTAAAATAT AGTGGTAATC CTACTGTCAA CACAGACTCA
    TCAGATCCTT GCATTGGTAC TTTGGAAAAG ATTATTGACG AGATGAACAA TAATAAAAGT
    GACTCTGTAA ATTTTAACTT GAAGGATGCC CTTTGTGATC AAAGCACAAG TCAAAACAAA
    AAGGAAAGTT ATGATTGCCT TCCGGTACAA TGCAGGACTA CACAATTACC GTTTATGGTT
    TCAGAGTGTG AAAGAAAGGA TAGGGAATCC CTTGACATAG AGTGTTCTGC ACTTATTAGA
    ATGTCTGCAA ATGAGTCAGT GGTCGATGAC TATTCAGATT TTTCACTTAC TGAGGAAAAT
    GTTAACTTTG TAAATAATAA CAATATAAAT GAGCATACAG AAGAATGCAA AACCTGTTCC
    TGTCAGAGAA CAATCCCATT TACTGGGAAA ACCATCTGGA AATGTTCTTG TGCAAGGACG
    TGTGTATGGG CTTTCCCAAG AAAAAGAACT TCCTCTTTAC CAAGTACAGA TATACTGGGA
    TTTGACTCTG AATTGTCTTT TGCCCCTTAT TCCACCAATA TAAAGTCTTT ATTTCAGGCT
    AATGAAGCAG AGCCATTGCA GAGCACAACT GATGACACTG ACAGTGACGG GATATGTAAA
    AAACTAAAGT TAGATTTCAT TTCAGAAAAT GACGCTCTTA TTCTTAAAGA TATTGATGAT
    GACATTTTGT CTCACCCTCC AACAGAAACT TTTGAACTAC CCAATACTGT AATTGAGAAG
    ATATGTAATA TAACTGAAAA TGTTTACTCA CTAGGTGAAG TTGAAGACAT GTATAAAAAT
    TTAGATGGTT GGCCATGCAG TTCAACTATT ATTCAAAATA CTATAAATAG TCTTCCTCTC
    CCGCTGCCAA ACAATGACAA AAGTGAGCAC TTTACCACTT CTGGCACTTA TTCTGAAATA
    GAACCTACTG TAAAAACAGT TGAGGAGATG GCCCTGAGCA GTTCTTTTGA AAACTCTGAT
    CCAAGTGTTT GTTCCAGAGA CAGCTCCACA GAACCTGAAA ACAGTTCAGA GACTGTGCTA
    AAAAGTACTC AATATGAATC TCCAATACGT GATTGTTTAG ACAAGCATAA AAAAAACACT
    AATGTTGAGG AAGTGTTATC CTTTTTTGAT TGTTTACATG AAACTACAAT GGATAACGCC
    TGTCTAGCTG AGGAAAATTG CACCAGACAG ATTAATAATA CTGGTGGCCC ATTAGATGAT
    TCACATTTGA CACTAAGCCA TGTAGAATCT CCAAAGAAAA GCAAGCTTGT GGAATACAAA
    AGTAATTTAG ATGTGATAAA AGCTTATGAA GAAGATGTAC TGTTTTTAGA TGTTGTTCCA
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa33112
    Clone ID Related Accession (Same CDS sequence) XM_012964412.2 , XM_012964413.2 , XM_012964411.2
    Accession Version XM_012964411.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product testis- and ovary-specific PAZ domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCAAAATC CATTGCTTGA AACAGCAAAC AATAGAAAAA GTAAATGTTC ATATGGTGGT 
    TCCCAGTCTT TTCTAAATCC TGTAGTGAAA TTGCATAACT GCAGCAACAT GAAGAGGCTT
    ATACGTTGCT CAAATCTGAG GGTAAGCAGT TCCAAAACTG AGAATACATG TCCTGCAGCA
    ACAGTAAAAG AACTTGATCC TCAAAAACAA TTACCTTGTT CTGTTAACAC TGTAAATTCA
    GTTATCAGTA CAGATTCTAC TGTTGGAGGT AATCATTCCA AAGAACTGGA ATCTATTGCA
    CATGGCTTGG CAAATAGGAA AATTAAAAGA ATATTAAAAG TAAAGTGGGA CTTTGGAAAA
    AAAATAAGGA ATTCTGCAAC ATGCTCTACA GAGCGTACTC AAGAAGACTC TGCAATATCT
    GTCAAACAGA AAACAGGAGA CCTAAAATAT AGTGGTAATC CTACTGTCAA CACAGACTCA
    TCAGATCCTT GCATTGGTAC TTTGGAAAAG ATTATTGACG AGATGAACAA TAATAAAAGT
    GACTCTGTAA ATTTTAACTT GAAGGATGCC CTTTGTGATC AAAGCACAAG TCAAAACAAA
    AAGGAAAGTT ATGATTGCCT TCCGGTACAA TGCAGGACTA CACAATTACC GTTTATGGTT
    TCAGAGTGTG AAAGAAAGGA TAGGGAATCC CTTGACATAG AGTGTTCTGC ACTTATTAGA
    ATGTCTGCAA ATGAGTCAGT GGTCGATGAC TATTCAGATT TTTCACTTAC TGAGGAAAAT
    GTTAACTTTG TAAATAATAA CAATATAAAT GAGCATACAG AAGAATGCAA AACCTGTTCC
    TGTCAGAGAA CAATCCCATT TACTGGGAAA ACCATCTGGA AATGTTCTTG TGCAAGGACG
    TGTGTATGGG CTTTCCCAAG AAAAAGAACT TCCTCTTTAC CAAGTACAGA TATACTGGGA
    TTTGACTCTG AATTGTCTTT TGCCCCTTAT TCCACCAATA TAAAGTCTTT ATTTCAGGCT
    AATGAAGCAG AGCCATTGCA GAGCACAACT GATGACACTG ACAGTGACGG GATATGTAAA
    AAACTAAAGT TAGATTTCAT TTCAGAAAAT GACGCTCTTA TTCTTAAAGA TATTGATGAT
    GACATTTTGT CTCACCCTCC AACAGAAACT TTTGAACTAC CCAATACTGT AATTGAGAAG
    ATATGTAATA TAACTGAAAA TGTTTACTCA CTAGGTGAAG TTGAAGACAT GTATAAAAAT
    TTAGATGGTT GGCCATGCAG TTCAACTATT ATTCAAAATA CTATAAATAG TCTTCCTCTC
    CCGCTGCCAA ACAATGACAA AAGTGAGCAC TTTACCACTT CTGGCACTTA TTCTGAAATA
    GAACCTACTG TAAAAACAGT TGAGGAGATG GCCCTGAGCA GTTCTTTTGA AAACTCTGAT
    CCAAGTGTTT GTTCCAGAGA CAGCTCCACA GAACCTGAAA ACAGTTCAGA GACTGTGCTA
    AAAAGTACTC AATATGAATC TCCAATACGT GATTGTTTAG ACAAGCATAA AAAAAACACT
    AATGTTGAGG AAGTGTTATC CTTTTTTGAT TGTTTACATG AAACTACAAT GGATAACGCC
    TGTCTAGCTG AGGAAAATTG CACCAGACAG ATTAATAATA CTGGTGGCCC ATTAGATGAT
    TCACATTTGA CACTAAGCCA TGTAGAATCT CCAAAGAAAA GCAAGCTTGT GGAATACAAA
    AGTAATTTAG ATGTGATAAA AGCTTATGAA GAAGATGTAC TGTTTTTAGA TGTTGTTCCA
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012819865.1
    CDS298..4527
    Translation

    Target ORF information:

    RefSeq Version XM_012964411.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012964411.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGCAAAATC CATTGCTTGA AACAGCAAAC AATAGAAAAA GTAAATGTTC ATATGGTGGT 
    TCCCAGTCTT TTCTAAATCC TGTAGTGAAA TTGCATAACT GCAGCAACAT GAAGAGGCTT
    ATACGTTGCT CAAATCTGAG GGTAAGCAGT TCCAAAACTG AGAATACATG TCCTGCAGCA
    ACAGTAAAAG AACTTGATCC TCAAAAACAA TTACCTTGTT CTGTTAACAC TGTAAATTCA
    GTTATCAGTA CAGATTCTAC TGTTGGAGGT AATCATTCCA AAGAACTGGA ATCTATTGCA
    CATGGCTTGG CAAATAGGAA AATTAAAAGA ATATTAAAAG TAAAGTGGGA CTTTGGAAAA
    AAAATAAGGA ATTCTGCAAC ATGCTCTACA GAGCGTACTC AAGAAGACTC TGCAATATCT
    GTCAAACAGA AAACAGGAGA CCTAAAATAT AGTGGTAATC CTACTGTCAA CACAGACTCA
    TCAGATCCTT GCATTGGTAC TTTGGAAAAG ATTATTGACG AGATGAACAA TAATAAAAGT
    GACTCTGTAA ATTTTAACTT GAAGGATGCC CTTTGTGATC AAAGCACAAG TCAAAACAAA
    AAGGAAAGTT ATGATTGCCT TCCGGTACAA TGCAGGACTA CACAATTACC GTTTATGGTT
    TCAGAGTGTG AAAGAAAGGA TAGGGAATCC CTTGACATAG AGTGTTCTGC ACTTATTAGA
    ATGTCTGCAA ATGAGTCAGT GGTCGATGAC TATTCAGATT TTTCACTTAC TGAGGAAAAT
    GTTAACTTTG TAAATAATAA CAATATAAAT GAGCATACAG AAGAATGCAA AACCTGTTCC
    TGTCAGAGAA CAATCCCATT TACTGGGAAA ACCATCTGGA AATGTTCTTG TGCAAGGACG
    TGTGTATGGG CTTTCCCAAG AAAAAGAACT TCCTCTTTAC CAAGTACAGA TATACTGGGA
    TTTGACTCTG AATTGTCTTT TGCCCCTTAT TCCACCAATA TAAAGTCTTT ATTTCAGGCT
    AATGAAGCAG AGCCATTGCA GAGCACAACT GATGACACTG ACAGTGACGG GATATGTAAA
    AAACTAAAGT TAGATTTCAT TTCAGAAAAT GACGCTCTTA TTCTTAAAGA TATTGATGAT
    GACATTTTGT CTCACCCTCC AACAGAAACT TTTGAACTAC CCAATACTGT AATTGAGAAG
    ATATGTAATA TAACTGAAAA TGTTTACTCA CTAGGTGAAG TTGAAGACAT GTATAAAAAT
    TTAGATGGTT GGCCATGCAG TTCAACTATT ATTCAAAATA CTATAAATAG TCTTCCTCTC
    CCGCTGCCAA ACAATGACAA AAGTGAGCAC TTTACCACTT CTGGCACTTA TTCTGAAATA
    GAACCTACTG TAAAAACAGT TGAGGAGATG GCCCTGAGCA GTTCTTTTGA AAACTCTGAT
    CCAAGTGTTT GTTCCAGAGA CAGCTCCACA GAACCTGAAA ACAGTTCAGA GACTGTGCTA
    AAAAGTACTC AATATGAATC TCCAATACGT GATTGTTTAG ACAAGCATAA AAAAAACACT
    AATGTTGAGG AAGTGTTATC CTTTTTTGAT TGTTTACATG AAACTACAAT GGATAACGCC
    TGTCTAGCTG AGGAAAATTG CACCAGACAG ATTAATAATA CTGGTGGCCC ATTAGATGAT
    TCACATTTGA CACTAAGCCA TGTAGAATCT CCAAAGAAAA GCAAGCTTGT GGAATACAAA
    AGTAATTTAG ATGTGATAAA AGCTTATGAA GAAGATGTAC TGTTTTTAGA TGTTGTTCCA
    GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT
    GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA
    CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA
    GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC
    ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT
    TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA
    TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT
    CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG
    ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT
    CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC
    TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA
    GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT
    CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT
    GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG
    CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT
    GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC
    TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG
    GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC
    CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG
    CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT
    AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT
    GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG
    CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC
    GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT
    CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA
    ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC
    TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA
    CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA
    ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA
    AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA
    ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA
    GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA
    CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG
    CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA
    GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG
    TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC
    CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT
    TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG
    CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT
    TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG
    AAAGTATGGC TTTTTCAGGG GAAAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.