eif4g1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_018094420.1	XP_017949909.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_004914398.4	XP_004914455.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_004914401.4	XP_004914458.1	eukaryotic translation initiation factor 4 gamma 1 isoform X2
XM_004914398.3	XP_004914455.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_004914400.3	XP_004914457.1	eukaryotic translation initiation factor 4 gamma 1 isoform X2
XM_004914401.3	XP_004914458.1	eukaryotic translation initiation factor 4 gamma 1 isoform X2
XM_012963378.2	XP_012818832.1	eukaryotic translation initiation factor 4 gamma 1 isoform X2
XM_004914402.3	XP_004914459.1	eukaryotic translation initiation factor 4 gamma 1 isoform X3
XM_002935144.5	XP_002935190.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_012963378.3	XP_012818832.1	eukaryotic translation initiation factor 4 gamma 1 isoform X2
XM_018094419.1	XP_017949908.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_012963377.3	XP_012818831.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_002935144.4	XP_002935190.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_004914400.4	XP_004914457.1	eukaryotic translation initiation factor 4 gamma 1 isoform X2
XM_012963377.2	XP_012818831.1	eukaryotic translation initiation factor 4 gamma 1 isoform X1
XM_004914402.4	XP_004914459.1	eukaryotic translation initiation factor 4 gamma 1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10204	XM_002935144.5 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa10204	XM_004914398.4 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa10204	XM_012963377.3 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa10205	XM_004914400.4 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa10205	XM_004914401.4 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa10205	XM_012963378.3 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa10206	XM_004914402.4 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa10204	XM_018094420.1 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa10204	XM_018094419.1 Latest version!	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OXa10204	XM_004914398.3	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa10205	XM_004914401.3	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa10205	XM_012963378.2	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa10204	XM_002935144.4	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa10205	XM_004914400.3	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa10204	XM_012963377.2	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa10206	XM_004914402.3	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

eif4g1 ( XM_004914398.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914455.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa10204
Clone ID Related Accession (Same CDS sequence)	XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
Accession Version	XM_004914398.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914398.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914455.1
CDS	137..4966
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQAGFRSIQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_004914398.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914398.4

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_002935144.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935190.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X1, mRNA.

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CloneID	OXa10204
Clone ID Related Accession (Same CDS sequence)	XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
Accession Version	XM_002935144.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935144.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935190.1
CDS	214..5043
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQAGFRSIQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_002935144.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935144.5

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_012963377.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818831.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa10204
Clone ID Related Accession (Same CDS sequence)	XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
Accession Version	XM_012963377.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963377.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818831.1
CDS	130..4959
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQAGFRSIQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_012963377.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963377.3

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_004914401.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914458.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X5, mRNA.

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CloneID	OXa10205
Clone ID Related Accession (Same CDS sequence)	XM_004914401.4 , XM_004914400.3 , XM_004914401.3 , XM_012963378.2 , XM_012963378.3 , XM_004914400.4
Accession Version	XM_004914401.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914401.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC 
ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG 
TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA 
TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG 
ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA 
GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG 
AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC 
AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG 
CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT 
CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC 
AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA 
AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG 
CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC 
CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG 
GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG 
CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT 
TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT 
CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA 
CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT 
GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC 
TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA 
CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC 
ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA 
GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC 
TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA 
CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC 
TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC 
CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT 
GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC 
CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT 
GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG 
GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG 
CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA 
CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT 
ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA 
CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA 
TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG 
AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA 
ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT 
TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC 
AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG 
TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG 
AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG 
TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG 
GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC 
ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC 
CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG 
GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA 
ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG 
CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC 
CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT 
CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA 
CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC 
AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT 
TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG 
GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA 
AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT 
CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT 
ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA 
GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT 
ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT 
AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT 
ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT 
ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC 
AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC 
TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG 
GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG 
GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG 
AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT 
ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA 
GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT 
AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC 
ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT 
TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT 
GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC 
CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA 
GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT 
GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT 
GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914458.1
CDS	146..4954
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_004914401.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914401.4

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC 
ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG 
TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA 
TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG 
ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA 
GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG 
AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC 
AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG 
CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT 
CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC 
AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA 
AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG 
CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC 
CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG 
GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG 
CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT 
TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT 
CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA 
CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT 
GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC 
TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA 
CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC 
ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA 
GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC 
TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA 
CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC 
TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC 
CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT 
GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC 
CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT 
GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG 
GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG 
CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA 
CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT 
ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA 
CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA 
TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG 
AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA 
ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT 
TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC 
AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG 
TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG 
AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG 
TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG 
GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC 
ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC 
CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG 
GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA 
ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG 
CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC 
CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT 
CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA 
CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC 
AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT 
TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG 
GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA 
AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT 
CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT 
ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA 
GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT 
ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT 
AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT 
ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT 
ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC 
AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC 
TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG 
GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG 
GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG 
AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT 
ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA 
GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT 
AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC 
ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT 
TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT 
GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC 
CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA 
GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT 
GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT 
GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_012963378.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818832.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X6, mRNA.

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CloneID	OXa10205
Clone ID Related Accession (Same CDS sequence)	XM_004914401.4 , XM_004914400.3 , XM_004914401.3 , XM_012963378.2 , XM_012963378.3 , XM_004914400.4
Accession Version	XM_012963378.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963378.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC 
ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG 
TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA 
TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG 
ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA 
GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG 
AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC 
AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG 
CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT 
CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC 
AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA 
AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG 
CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC 
CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG 
GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG 
CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT 
TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT 
CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA 
CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT 
GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC 
TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA 
CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC 
ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA 
GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC 
TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA 
CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC 
TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC 
CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT 
GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC 
CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT 
GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG 
GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG 
CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA 
CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT 
ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA 
CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA 
TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG 
AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA 
ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT 
TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC 
AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG 
TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG 
AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG 
TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG 
GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC 
ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC 
CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG 
GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA 
ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG 
CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC 
CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT 
CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA 
CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC 
AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT 
TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG 
GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA 
AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT 
CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT 
ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA 
GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT 
ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT 
AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT 
ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT 
ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC 
AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC 
TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG 
GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG 
GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG 
AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT 
ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA 
GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT 
AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC 
ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT 
TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT 
GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC 
CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA 
GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT 
GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT 
GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818832.1
CDS	341..5149
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_012963378.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963378.3

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC 
ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG 
TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA 
TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG 
ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA 
GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG 
AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC 
AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG 
CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT 
CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC 
AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA 
AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG 
CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC 
CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG 
GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG 
CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT 
TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT 
CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA 
CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT 
GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC 
TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA 
CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC 
ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA 
GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC 
TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA 
CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC 
TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC 
CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT 
GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC 
CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT 
GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG 
GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG 
CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA 
CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT 
ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA 
CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA 
TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG 
AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA 
ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT 
TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC 
AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG 
TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG 
AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG 
TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG 
GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC 
ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC 
CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG 
GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA 
ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG 
CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC 
CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT 
CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA 
CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC 
AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT 
TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG 
GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA 
AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT 
CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT 
ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA 
GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT 
ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT 
AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT 
ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT 
ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC 
AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC 
TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG 
GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG 
GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG 
AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT 
ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA 
GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT 
AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC 
ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT 
TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT 
GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC 
CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA 
GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT 
GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT 
GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_004914400.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914457.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X4, mRNA.

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CloneID	OXa10205
Clone ID Related Accession (Same CDS sequence)	XM_004914401.4 , XM_004914400.3 , XM_004914401.3 , XM_012963378.2 , XM_012963378.3 , XM_004914400.4
Accession Version	XM_004914400.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914400.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC 
ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG 
TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA 
TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG 
ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA 
GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG 
AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC 
AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG 
CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT 
CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC 
AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA 
AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG 
CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC 
CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG 
GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG 
CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT 
TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT 
CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA 
CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT 
GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC 
TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA 
CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC 
ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA 
GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC 
TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA 
CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC 
TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC 
CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT 
GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC 
CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT 
GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG 
GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG 
CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA 
CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT 
ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA 
CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA 
TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG 
AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA 
ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT 
TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC 
AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG 
TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG 
AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG 
TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG 
GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC 
ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC 
CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG 
GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA 
ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG 
CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC 
CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT 
CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA 
CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC 
AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT 
TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG 
GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA 
AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT 
CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT 
ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA 
GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT 
ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT 
AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT 
ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT 
ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC 
AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC 
TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG 
GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG 
GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG 
AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT 
ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA 
GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT 
AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC 
ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT 
TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT 
GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC 
CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA 
GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT 
GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT 
GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914457.1
CDS	213..5021
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_004914400.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914400.4

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC 
ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG 
TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA 
TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG 
ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA 
GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG 
AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC 
AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG 
CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT 
CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC 
AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA 
AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG 
CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC 
CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG 
GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG 
CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT 
TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT 
CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA 
CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT 
GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC 
TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA 
CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC 
ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA 
GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC 
TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA 
CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC 
TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC 
CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT 
GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC 
CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT 
GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG 
GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG 
CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA 
CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT 
ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA 
CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA 
TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG 
AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA 
ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT 
TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC 
AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG 
TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG 
AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG 
TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG 
GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC 
ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC 
CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG 
GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA 
ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG 
CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC 
CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT 
CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA 
CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC 
AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT 
TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG 
GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA 
AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT 
CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT 
ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA 
GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT 
ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT 
AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT 
ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT 
ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC 
AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC 
TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG 
GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG 
GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG 
AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT 
ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA 
GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT 
AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC 
ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT 
TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT 
GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC 
CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA 
GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT 
GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT 
GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_004914402.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914459.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X7, mRNA.

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CloneID	OXa10206
Clone ID Related Accession (Same CDS sequence)	XM_004914402.3 , XM_004914402.4
Accession Version	XM_004914402.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004914402.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GGACGCTCAT ATGTGGTGCC CACAAACCAG 
TACCCTGTGC AGACTGGGGC TCCAGGTTTC TTCCCCAGTG CAAGTCCCTC AGAATATGGC 
ACATATGCTG GAGCATATTA CCCTGCACAA CAGACGTTCC CTCCTGGAGT GCCTGGAGCA 
CAAGTTATGA TGAATCAGCC ACAGGTGATC CCTCCTAAAC GGGAGCGCAA AACGATAAGA 
ATTAGAGACC CCAACCAAGG AGGCAAAGAT ATCACAGAAG AAATTCTCTC AGGAGGAAGA 
ACATCCTCTA CGCCAACGCC TCCACAGTCA ACTGTGGGAA GTTCTGAAAT TCAAGCTAAT 
GGAGAAAGCG TTCATCATGT TACCGTTGTT GTGCGCCCAG ATGATGGACC CAAGGCCACC 
CAAGTGCTGA TCAAAGCCTC AGAGAGCCCT TCCCCTCCTC TGCCTCTCTC TGAAGCTGAG 
CCAAGCATAG CAAATGTGTG TGATATTTCA AAAGATAACA CTATCTCTGA GTTGACTTCT 
CATATAGTAG AGCCCCTAAG TTCAGAAGAG ACAACCATTG ATACTTCTAC CTCTCAGGTG 
GATCTCAAAC ACCAGCCAGT GGCTGCTATG GCAGAGCATG AAACTCTTAT AGAGGAGGCA 
TCAATACAGG AGGTGGCCTT TGCAGAGGCA CCCATCGCTC AATCAGAGAT ACCAAAATCT 
CTTGCCTCCC AGCCTCTAGC AGAAATCTCT ACCCCTGAAC AGATTTCTAA ACTTGACGCA 
CAAGTGCCAT CTTTGCAACA AGAGGAGTTA CTTACAGAAA CTGTTGAACT TAATTTAAGC 
AATCAGGCTC TTCCTTCACA AATTTCTGAG CCTGAACCTT TTGAACCTGA GGCTTCAGCT 
TCAGATCCTC TACATGAGGA GCCCACAGAG CCCACAGTTT CAACAGAAGA ACCACAAGCT 
AATGGACTGT CTGAGGAGAC ACCAGTATTA CCTGTACAGC CACAACCAAC ATCTGTTTGC 
CAGACTGAGC CCTCCCTAGA ATCCCCCATC GCTCAGCCTG AGGAGCTGCT TCTAACAAAT 
GGAGGTGGAC CACCTGGCCT ACAAGAAAAT GATGCACCTG ATTCACAGGT TGAAGCTCAT 
GTTAGCCCAA TCACTGAGCC TGAGGACCAA CCTGTGACAG TCTCTGATAA CGTGCTTGTG 
GAAGAGGATG TAGAAAAAAC ATTGCCTGCG GATACGGAAG TCGTGGCTTC AACCCAAGTT 
GTAAAGCAGA GCTCCGAGAG TCAGTCTCAG GTTGCTGTTG CTGCACCCAA GAAAAAGCGA 
AAAATGAAGG AACTAAACAA GAAAGAGGCA GTGGGAGATC TCCTTGATGC GTTCAAAGAG 
GCTGGTGATG GCTCTTCTGA TGTGGATTCT TCCTCTAAGC CTACCAGTGC TGCACCTGAA 
CCAGATCAGA GCCCTTGTGC TCCTCCTGAG GAACAGGATG AAACTTGGGA AGAGAAGGAA 
GATAAACTGG ATGCAGAGAA TATTAAACCA GGAGAACATA AATATAAATA CAAAGAAGAG 
CAATGGAAAC CCCTAAACCC GGAGGAAAAG AAGAGTTATG ATCGCGAGTT TCTGCTAGGG 
TTCCAGTTTA TTGTGGCCAG TATGCAGAAA CCTGCGGGTC TTCCTCAAAT TACTGATGTG 
GTGCTGGATA AGGCAAACAA AACTCCATTG CGACCATTGG ATCCTACACG TCTGACGGGA 
ATGAACTGTG GGCCAGATTT TACACCTTCA TTTGCCAACC TTGGTCGGCC TGCTGTGGGT 
AACCGTGGAC CACCAGCTGG CATGGGTCCG AGACGGTCCC AGCAGGGTCC ACGAAAAGAG 
CCTCGTAAAA TTATTCAAAC CGTATCATTA AATGAGAATG TACAACTAAA CAAAGCTGAA 
AAGGCATGGA AACCGATAAA CAAGAGAGCT GGCGATGAGG AGGACCCAGA AAATATTAAG 
ACACAGGACC TATTCCGAAG GGTTCGCAGT ATCTTGAATA AGCTGACCCC TCAGATGTTC 
CAGCAGCTGA TGAAGCAGGT CACTGACCTT ACTATTGATA CCGAAGATCG TCTTAAGGGT 
GTCATTGACC TAATTTTTGA AAAAGCCATT TCAGAACCCA ATTTCTCTGT TGCATATGCC 
AACATGTGTC GTTGCCTTAT GGGGCTTAAA GTTCCAACCA CAGACAAGCC AGGGGTTACA 
GTCAACTTCC GCAAGTTGTT ACTGAATCGG TGCCAGAAGG AGTTTGAGAA GGATAAGGAT 
GATGATGAAG TGTTTGAGAA GAAACAAAAA GAAATGGATG CTGCTGCTTC GCCGGAGGAA 
AAAGCACGTT TGAATGATGA ACTGGTCGAG GCCCGTGACA GTGCACGACG GCGATCCTTG 
GGGAACATTA AGTTCATTGG AGAACTGTTT AAGTTAAAAA TGCTGACTGA AGCTATAATG 
CACGATTGTG TGGTGAAGCT ATTAAAAAAT CATGATGAGG AATCTCTGGA GTGCCTCTGC 
CGACTGCTGT CCACCATTGG CAAGGACCTG GATTTTGAGA GAGCTAAGCC ACGTATGGAC 
CAGTACTTCA ACCAGATGGA TAAAATCATT AAGGAGCGGA AGACATCTTC ACGAATTAGA 
TTCATGCTGC AGGATGTGAT TGATTTGCGT CTGTGTAATT GGGTGCCTCG TCGTGCAGAT 
CAGGGCCCTA AAACTATAGA TCAGATTCAT AAAGAAGCAG AAATGGAAAA GCAGAGGGAG 
CAGGTTAAGG TGCAACAACT GATGTCTAAA CAAGACAAGC GGCGAGAGCC CCCAGGACGA 
CCCACTGGAA GCCGGGGTAA CCAGCCTGCA GATGATGGCT GGAATACAGT GCCCATCAGT 
AAGGGCAGCC GTCCTATTGA CACCACTAGG ATCACAAAGA TAACAAAGCT TGGTGCTATT 
GATTCAAATC AACTGCTTGC CCCAGGAGGA CGTCTGAGTT GGGGTAAAGG AAGCAGCGGA 
GGAACTGGAG CCAAACCAGC AGAAACAGCT CCAGAATCTA GTCGGCCTGC GACCAGCACC 
TTAAACAGGT TTTCTGCCTT GCAGCAGTCT TCATCTGCTG AGAGTCAAGA TGCTAGACGG 
CCCTTGCATC GGGGAAGTTC TAGTCGGGAA CGTTCTGAAA AAGATCGTGG AGACAGATTT 
GATAGAGGTG AAAGGTTTGA TAAGGGAGAA CTGTTTGATA GATCTGACCG TTCAGATCGA 
GCTCGTCCTC TTCTTACAAA GAGAAGCTAT AGCAAGGAAA CTGAGGACCG TAGCCGAGAA 
AGAGAGGCCC ATATACCACA GGAGGCTGTG AGGAAGGTGG CAAGTATGAC TGAGCAGCGG 
GACCGCAGCA AAGAAAGTGT GAAAAAAGAA ATTCCAGCAC CGACAGCAGT GGTTGCCTCA 
TCAAAGCCCT CTATGTCTGA GGATGAGCTA GAGAAGAAAT CTAAAGCCAT CATAGAAGAA 
TATTTGCACA TTAATGATAT GAAGGAAGCT CTTCAGTGTG TAGTGGAGCT TAATAGTTCA 
ACGCTGCTTT TTATCTTTGT GCGGAATGGG ATTGAGTCAA CACTAGAGAG AAGCACTATT 
GCCAGAGAGC ATATGGGTCT CCTTTTGTAC CAGTTAGTAA AGGCAGGAAC TCTGTCCAAG 
GAGCAGTATT ACAAAGGGGT GCTTGAAGTT CTTGAAGTAG CAGAAGATAT GGAAATAGAT 
ATACCCCACA TCTGGCTGTA TTTAGCAGAG ATAATCAGCC CTGTGCTGCT AGAGGGGGGG 
ATTCCGATGG GGGAGCTGTT CAGAGAGCTG ACCAAACCTT TAGTGCTCAT TGGCAAAGCT 
GGAGTCCTTC TGGCAGAAAT CCTTGGATTA TTGTGCAAAG GCATGAGTCA TAAGAAGGCT 
GGAGCCCTGT GGAAAGAATC TGGGCTAAGC TGGAAGGAAT TCTTGTCCAA GGACCAGGAT 
ATTAACAAGT TTATAACTGA CAAGAAACTC GAATACACAA CAGGAGAGGA GACTGAAACA 
CCCAGTAAGA AAGACCTCAC CCCTGCTGAA CTTGATAAAA TTTTGACAAA ACTTTTGAAA 
GAGAACAGCA GTAACCAGAA GATATTTGAC TGGATTGAAG CCAACTTAAA TGACCAGCAA 
ACTTCATCCA ACACCTTTGT TCGAACTTTA ATGAGTACTG TGTGCCACTC TGCTATTATT 
TTTGAGAATC CTTGTAGGGT TGATGTTAAT ATTATCCATA CTAGAGCCAA ATTTCTTCAG 
AAATATTTAA GTGACGAGGC CAAGGAACTA CAGGCTCTCT ATGCTCTACA GGCACTTGTT 
GTAAAGCTTG ACCAGCCCCC AAATCTCCTA CGGATGTTTT TTGATGCTCT CTATGATGAG 
GATGTGATAA AAGAGGATCC ATTCTACAAG TGGGAGTCCA GCAAAGACCC AGCTGAACAG 
CAAGGAAAAG GTGTTGCTCT TAAATCTGTC ACTGCCTTCT TCACATGGCT ACGGGAAGCA 
GAGGATGAGT CTGATAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914459.1
CDS	214..4854
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_004914402.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914402.4

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GGACGCTCAT ATGTGGTGCC CACAAACCAG 
TACCCTGTGC AGACTGGGGC TCCAGGTTTC TTCCCCAGTG CAAGTCCCTC AGAATATGGC 
ACATATGCTG GAGCATATTA CCCTGCACAA CAGACGTTCC CTCCTGGAGT GCCTGGAGCA 
CAAGTTATGA TGAATCAGCC ACAGGTGATC CCTCCTAAAC GGGAGCGCAA AACGATAAGA 
ATTAGAGACC CCAACCAAGG AGGCAAAGAT ATCACAGAAG AAATTCTCTC AGGAGGAAGA 
ACATCCTCTA CGCCAACGCC TCCACAGTCA ACTGTGGGAA GTTCTGAAAT TCAAGCTAAT 
GGAGAAAGCG TTCATCATGT TACCGTTGTT GTGCGCCCAG ATGATGGACC CAAGGCCACC 
CAAGTGCTGA TCAAAGCCTC AGAGAGCCCT TCCCCTCCTC TGCCTCTCTC TGAAGCTGAG 
CCAAGCATAG CAAATGTGTG TGATATTTCA AAAGATAACA CTATCTCTGA GTTGACTTCT 
CATATAGTAG AGCCCCTAAG TTCAGAAGAG ACAACCATTG ATACTTCTAC CTCTCAGGTG 
GATCTCAAAC ACCAGCCAGT GGCTGCTATG GCAGAGCATG AAACTCTTAT AGAGGAGGCA 
TCAATACAGG AGGTGGCCTT TGCAGAGGCA CCCATCGCTC AATCAGAGAT ACCAAAATCT 
CTTGCCTCCC AGCCTCTAGC AGAAATCTCT ACCCCTGAAC AGATTTCTAA ACTTGACGCA 
CAAGTGCCAT CTTTGCAACA AGAGGAGTTA CTTACAGAAA CTGTTGAACT TAATTTAAGC 
AATCAGGCTC TTCCTTCACA AATTTCTGAG CCTGAACCTT TTGAACCTGA GGCTTCAGCT 
TCAGATCCTC TACATGAGGA GCCCACAGAG CCCACAGTTT CAACAGAAGA ACCACAAGCT 
AATGGACTGT CTGAGGAGAC ACCAGTATTA CCTGTACAGC CACAACCAAC ATCTGTTTGC 
CAGACTGAGC CCTCCCTAGA ATCCCCCATC GCTCAGCCTG AGGAGCTGCT TCTAACAAAT 
GGAGGTGGAC CACCTGGCCT ACAAGAAAAT GATGCACCTG ATTCACAGGT TGAAGCTCAT 
GTTAGCCCAA TCACTGAGCC TGAGGACCAA CCTGTGACAG TCTCTGATAA CGTGCTTGTG 
GAAGAGGATG TAGAAAAAAC ATTGCCTGCG GATACGGAAG TCGTGGCTTC AACCCAAGTT 
GTAAAGCAGA GCTCCGAGAG TCAGTCTCAG GTTGCTGTTG CTGCACCCAA GAAAAAGCGA 
AAAATGAAGG AACTAAACAA GAAAGAGGCA GTGGGAGATC TCCTTGATGC GTTCAAAGAG 
GCTGGTGATG GCTCTTCTGA TGTGGATTCT TCCTCTAAGC CTACCAGTGC TGCACCTGAA 
CCAGATCAGA GCCCTTGTGC TCCTCCTGAG GAACAGGATG AAACTTGGGA AGAGAAGGAA 
GATAAACTGG ATGCAGAGAA TATTAAACCA GGAGAACATA AATATAAATA CAAAGAAGAG 
CAATGGAAAC CCCTAAACCC GGAGGAAAAG AAGAGTTATG ATCGCGAGTT TCTGCTAGGG 
TTCCAGTTTA TTGTGGCCAG TATGCAGAAA CCTGCGGGTC TTCCTCAAAT TACTGATGTG 
GTGCTGGATA AGGCAAACAA AACTCCATTG CGACCATTGG ATCCTACACG TCTGACGGGA 
ATGAACTGTG GGCCAGATTT TACACCTTCA TTTGCCAACC TTGGTCGGCC TGCTGTGGGT 
AACCGTGGAC CACCAGCTGG CATGGGTCCG AGACGGTCCC AGCAGGGTCC ACGAAAAGAG 
CCTCGTAAAA TTATTCAAAC CGTATCATTA AATGAGAATG TACAACTAAA CAAAGCTGAA 
AAGGCATGGA AACCGATAAA CAAGAGAGCT GGCGATGAGG AGGACCCAGA AAATATTAAG 
ACACAGGACC TATTCCGAAG GGTTCGCAGT ATCTTGAATA AGCTGACCCC TCAGATGTTC 
CAGCAGCTGA TGAAGCAGGT CACTGACCTT ACTATTGATA CCGAAGATCG TCTTAAGGGT 
GTCATTGACC TAATTTTTGA AAAAGCCATT TCAGAACCCA ATTTCTCTGT TGCATATGCC 
AACATGTGTC GTTGCCTTAT GGGGCTTAAA GTTCCAACCA CAGACAAGCC AGGGGTTACA 
GTCAACTTCC GCAAGTTGTT ACTGAATCGG TGCCAGAAGG AGTTTGAGAA GGATAAGGAT 
GATGATGAAG TGTTTGAGAA GAAACAAAAA GAAATGGATG CTGCTGCTTC GCCGGAGGAA 
AAAGCACGTT TGAATGATGA ACTGGTCGAG GCCCGTGACA GTGCACGACG GCGATCCTTG 
GGGAACATTA AGTTCATTGG AGAACTGTTT AAGTTAAAAA TGCTGACTGA AGCTATAATG 
CACGATTGTG TGGTGAAGCT ATTAAAAAAT CATGATGAGG AATCTCTGGA GTGCCTCTGC 
CGACTGCTGT CCACCATTGG CAAGGACCTG GATTTTGAGA GAGCTAAGCC ACGTATGGAC 
CAGTACTTCA ACCAGATGGA TAAAATCATT AAGGAGCGGA AGACATCTTC ACGAATTAGA 
TTCATGCTGC AGGATGTGAT TGATTTGCGT CTGTGTAATT GGGTGCCTCG TCGTGCAGAT 
CAGGGCCCTA AAACTATAGA TCAGATTCAT AAAGAAGCAG AAATGGAAAA GCAGAGGGAG 
CAGGTTAAGG TGCAACAACT GATGTCTAAA CAAGACAAGC GGCGAGAGCC CCCAGGACGA 
CCCACTGGAA GCCGGGGTAA CCAGCCTGCA GATGATGGCT GGAATACAGT GCCCATCAGT 
AAGGGCAGCC GTCCTATTGA CACCACTAGG ATCACAAAGA TAACAAAGCT TGGTGCTATT 
GATTCAAATC AACTGCTTGC CCCAGGAGGA CGTCTGAGTT GGGGTAAAGG AAGCAGCGGA 
GGAACTGGAG CCAAACCAGC AGAAACAGCT CCAGAATCTA GTCGGCCTGC GACCAGCACC 
TTAAACAGGT TTTCTGCCTT GCAGCAGTCT TCATCTGCTG AGAGTCAAGA TGCTAGACGG 
CCCTTGCATC GGGGAAGTTC TAGTCGGGAA CGTTCTGAAA AAGATCGTGG AGACAGATTT 
GATAGAGGTG AAAGGTTTGA TAAGGGAGAA CTGTTTGATA GATCTGACCG TTCAGATCGA 
GCTCGTCCTC TTCTTACAAA GAGAAGCTAT AGCAAGGAAA CTGAGGACCG TAGCCGAGAA 
AGAGAGGCCC ATATACCACA GGAGGCTGTG AGGAAGGTGG CAAGTATGAC TGAGCAGCGG 
GACCGCAGCA AAGAAAGTGT GAAAAAAGAA ATTCCAGCAC CGACAGCAGT GGTTGCCTCA 
TCAAAGCCCT CTATGTCTGA GGATGAGCTA GAGAAGAAAT CTAAAGCCAT CATAGAAGAA 
TATTTGCACA TTAATGATAT GAAGGAAGCT CTTCAGTGTG TAGTGGAGCT TAATAGTTCA 
ACGCTGCTTT TTATCTTTGT GCGGAATGGG ATTGAGTCAA CACTAGAGAG AAGCACTATT 
GCCAGAGAGC ATATGGGTCT CCTTTTGTAC CAGTTAGTAA AGGCAGGAAC TCTGTCCAAG 
GAGCAGTATT ACAAAGGGGT GCTTGAAGTT CTTGAAGTAG CAGAAGATAT GGAAATAGAT 
ATACCCCACA TCTGGCTGTA TTTAGCAGAG ATAATCAGCC CTGTGCTGCT AGAGGGGGGG 
ATTCCGATGG GGGAGCTGTT CAGAGAGCTG ACCAAACCTT TAGTGCTCAT TGGCAAAGCT 
GGAGTCCTTC TGGCAGAAAT CCTTGGATTA TTGTGCAAAG GCATGAGTCA TAAGAAGGCT 
GGAGCCCTGT GGAAAGAATC TGGGCTAAGC TGGAAGGAAT TCTTGTCCAA GGACCAGGAT 
ATTAACAAGT TTATAACTGA CAAGAAACTC GAATACACAA CAGGAGAGGA GACTGAAACA 
CCCAGTAAGA AAGACCTCAC CCCTGCTGAA CTTGATAAAA TTTTGACAAA ACTTTTGAAA 
GAGAACAGCA GTAACCAGAA GATATTTGAC TGGATTGAAG CCAACTTAAA TGACCAGCAA 
ACTTCATCCA ACACCTTTGT TCGAACTTTA ATGAGTACTG TGTGCCACTC TGCTATTATT 
TTTGAGAATC CTTGTAGGGT TGATGTTAAT ATTATCCATA CTAGAGCCAA ATTTCTTCAG 
AAATATTTAA GTGACGAGGC CAAGGAACTA CAGGCTCTCT ATGCTCTACA GGCACTTGTT 
GTAAAGCTTG ACCAGCCCCC AAATCTCCTA CGGATGTTTT TTGATGCTCT CTATGATGAG 
GATGTGATAA AAGAGGATCC ATTCTACAAG TGGGAGTCCA GCAAAGACCC AGCTGAACAG 
CAAGGAAAAG GTGTTGCTCT TAAATCTGTC ACTGCCTTCT TCACATGGCT ACGGGAAGCA 
GAGGATGAGT CTGATAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_018094420.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949909.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa10204
Clone ID Related Accession (Same CDS sequence)	XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
Accession Version	XM_018094420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949909.1
CDS	491..5320
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQAGFRSIQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_018094420.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094420.1

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_004914398.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914455.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X2, mRNA.

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CloneID	OXa10204
Clone ID Related Accession (Same CDS sequence)	XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
Accession Version	XM_004914398.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914455.1
CDS	195..5024
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQAGFRSIQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_004914398.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914398.3

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_018094419.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949908.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X4, mRNA.

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CloneID	OXa10204
Clone ID Related Accession (Same CDS sequence)	XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
Accession Version	XM_018094419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949908.1
CDS	226..5055
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQAGFRSIQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_018094419.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094419.1

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_002935144.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002935190.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X1, mRNA.

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CloneID	OXa10204
Clone ID Related Accession (Same CDS sequence)	XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
Accession Version	XM_002935144.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935144.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002935190.1
CDS	216..5045
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQAGFRSIQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_002935144.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002935144.4

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_012963377.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818831.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X3, mRNA.

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CloneID	OXa10204
Clone ID Related Accession (Same CDS sequence)	XM_018094420.1 , XM_004914398.4 , XM_004914398.3 , XM_002935144.5 , XM_018094419.1 , XM_012963377.3 , XM_002935144.4 , XM_012963377.2
Accession Version	XM_012963377.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963377.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818831.1
CDS	136..4965
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQAGFRSIQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_012963377.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963377.2

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GCAGGATTCA GATCAATCCA GCATTATTAT 
CAAAATAGGA CACAGCCTCC CACTAGTGCC ACCAGAGTGC AGAGTGGTTC TTCTGCTCGA 
GCAGTTACTC CAGCCCATGT GTATCAACCA GGCTCCCAAG TTATGATGAT TCCTTCACAG 
ATTTCATACC CTTCCCAGGG ATACTACATT CAGGGACAGG GACGCTCATA TGTGGTGCCC 
ACAAACCAGT ACCCTGTGCA GACTGGGGCT CCAGGTTTCT TCCCCAGTGC AAGTCCCTCA 
GAATATGGCA CATATGCTGG AGCATATTAC CCTGCACAAC AGACGTTCCC TCCTGGAGTG 
CCTGGAGCAC AAGTTATGAT GAATCAGCCA CAGGTGATCC CTCCTAAACG GGAGCGCAAA 
ACGATAAGAA TTAGAGACCC CAACCAAGGA GGCAAAGATA TCACAGAAGA AATTCTCTCA 
GGAGGAAGAA CATCCTCTAC GCCAACGCCT CCACAGTCAA CTGTGGGAAG TTCTGAAATT 
CAAGCTAATG GAGAAAGCGT TCATCATGTT ACCGTTGTTG TGCGCCCAGA TGATGGACCC 
AAGGCCACCC AAGTGCTGAT CAAAGCCTCA GAGAGCCCTT CCCCTCCTCT GCCTCTCTCT 
GAAGCTGAGC CAAGCATAGC AAATGTGTGT GATATTTCAA AAGATAACAC TATCTCTGAG 
TTGACTTCTC ATATAGTAGA GCCCCTAAGT TCAGAAGAGA CAACCATTGA TACTTCTACC 
TCTCAGGTGG ATCTCAAACA CCAGCCAGTG GCTGCTATGG CAGAGCATGA AACTCTTATA 
GAGGAGGCAT CAATACAGGA GGTGGCCTTT GCAGAGGCAC CCATCGCTCA ATCAGAGATA 
CCAAAATCTC TTGCCTCCCA GCCTCTAGCA GAAATCTCTA CCCCTGAACA GATTTCTAAA 
CTTGACGCAC AAGTGCCATC TTTGCAACAA GAGGAGTTAC TTACAGAAAC TGTTGAACTT 
AATTTAAGCA ATCAGGCTCT TCCTTCACAA ATTTCTGAGC CTGAACCTTT TGAACCTGAG 
GCTTCAGCTT CAGATCCTCT ACATGAGGAG CCCACAGAGC CCACAGTTTC AACAGAAGAA 
CCACAAGCTA ATGGACTGTC TGAGGAGACA CCAGTATTAC CTGTACAGCC ACAACCAACA 
TCTGTTTGCC AGACTGAGCC CTCCCTAGAA TCCCCCATCG CTCAGCCTGA GGAGCTGCTT 
CTAACAAATG GAGGTGGACC ACCTGGCCTA CAAGAAAATG ATGCACCTGA TTCACAGGTT 
GAAGCTCATG TTAGCCCAAT CACTGAGCCT GAGGACCAAC CTGTGACAGT CTCTGATAAC 
GTGCTTGTGG AAGAGGATGT AGAAAAAACA TTGCCTGCGG ATACGGAAGT CGTGGCTTCA 
ACCCAAGTTG TAAAGCAGAG CTCCGAGAGT CAGTCTCAGG TTGCTGTTGC TGCACCCAAG 
AAAAAGCGAA AAATGAAGGA ACTAAACAAG AAAGAGGCAG TGGGAGATCT CCTTGATGCG 
TTCAAAGAGG CTGGTGATGG CTCTTCTGAT GTGGATTCTT CCTCTAAGCC TACCAGTGCT 
GCACCTGAAC CAGATCAGAG CCCTTGTGCT CCTCCTGAGG AACAGGATGA AACTTGGGAA 
GAGAAGGAAG ATAAACTGGA TGCAGAGAAT ATTAAACCAG GAGAACATAA ATATAAATAC 
AAAGAAGAGC AATGGAAACC CCTAAACCCG GAGGAAAAGA AGAGTTATGA TCGCGAGTTT 
CTGCTAGGGT TCCAGTTTAT TGTGGCCAGT ATGCAGAAAC CTGCGGGTCT TCCTCAAATT 
ACTGATGTGG TGCTGGATAA GGCAAACAAA ACTCCATTGC GACCATTGGA TCCTACACGT 
CTGACGGGAA TGAACTGTGG GCCAGATTTT ACACCTTCAT TTGCCAACCT TGGTCGGCCT 
GCTGTGGGTA ACCGTGGACC ACCAGCTGGC ATGGGTCCGA GACGGTCCCA GCAGGGTCCA 
CGAAAAGAGC CTCGTAAAAT TATTCAAACC GTATCATTAA ATGAGAATGT ACAACTAAAC 
AAAGCTGAAA AGGCATGGAA ACCGATAAAC AAGAGAGCTG GCGATGAGGA GGACCCAGAA 
AATATTAAGA CACAGGACCT ATTCCGAAGG GTTCGCAGTA TCTTGAATAA GCTGACCCCT 
CAGATGTTCC AGCAGCTGAT GAAGCAGGTC ACTGACCTTA CTATTGATAC CGAAGATCGT 
CTTAAGGGTG TCATTGACCT AATTTTTGAA AAAGCCATTT CAGAACCCAA TTTCTCTGTT 
GCATATGCCA ACATGTGTCG TTGCCTTATG GGGCTTAAAG TTCCAACCAC AGACAAGCCA 
GGGGTTACAG TCAACTTCCG CAAGTTGTTA CTGAATCGGT GCCAGAAGGA GTTTGAGAAG 
GATAAGGATG ATGATGAAGT GTTTGAGAAG AAACAAAAAG AAATGGATGC TGCTGCTTCG 
CCGGAGGAAA AAGCACGTTT GAATGATGAA CTGGTCGAGG CCCGTGACAG TGCACGACGG 
CGATCCTTGG GGAACATTAA GTTCATTGGA GAACTGTTTA AGTTAAAAAT GCTGACTGAA 
GCTATAATGC ACGATTGTGT GGTGAAGCTA TTAAAAAATC ATGATGAGGA ATCTCTGGAG 
TGCCTCTGCC GACTGCTGTC CACCATTGGC AAGGACCTGG ATTTTGAGAG AGCTAAGCCA 
CGTATGGACC AGTACTTCAA CCAGATGGAT AAAATCATTA AGGAGCGGAA GACATCTTCA 
CGAATTAGAT TCATGCTGCA GGATGTGATT GATTTGCGTC TGTGTAATTG GGTGCCTCGT 
CGTGCAGATC AGGGCCCTAA AACTATAGAT CAGATTCATA AAGAAGCAGA AATGGAAAAG 
CAGAGGGAGC AGGTTAAGGT GCAACAACTG ATGTCTAAAC AAGACAAGCG GCGAGAGCCC 
CCAGGACGAC CCACTGGAAG CCGGGGTAAC CAGCCTGCAG ATGATGGCTG GAATACAGTG 
CCCATCAGTA AGGGCAGCCG TCCTATTGAC ACCACTAGGA TCACAAAGAT AACAAAGCTT 
GGTGCTATTG ATTCAAATCA ACTGCTTGCC CCAGGAGGAC GTCTGAGTTG GGGTAAAGGA 
AGCAGCGGAG GAACTGGAGC CAAACCAGCA GAAACAGCTC CAGAATCTAG TCGGCCTGCG 
ACCAGCACCT TAAACAGGTT TTCTGCCTTG CAGCAGTCTT CATCTGCTGA GAGTCAAGAT 
GCTAGACGGC CCTTGCATCG GGGAAGTTCT AGTCGGGAAC GTTCTGAAAA AGATCGTGGA 
GACAGATTTG ATAGAGGTGA AAGGTTTGAT AAGGGAGAAC TGTTTGATAG ATCTGACCGT 
TCAGATCGAG CTCGTCCTCT TCTTACAAAG AGAAGCTATA GCAAGGAAAC TGAGGACCGT 
AGCCGAGAAA GAGAGGCCCA TATACCACAG GAGGCTGTGA GGAAGGTGGC AAGTATGACT 
GAGCAGCGGG ACCGCAGCAA AGAAAGTGTG AAAAAAGAAA TTCCAGCACC GACAGCAGTG 
GTTGCCTCAT CAAAGCCCTC TATGTCTGAG GATGAGCTAG AGAAGAAATC TAAAGCCATC 
ATAGAAGAAT ATTTGCACAT TAATGATATG AAGGAAGCTC TTCAGTGTGT AGTGGAGCTT 
AATAGTTCAA CGCTGCTTTT TATCTTTGTG CGGAATGGGA TTGAGTCAAC ACTAGAGAGA 
AGCACTATTG CCAGAGAGCA TATGGGTCTC CTTTTGTACC AGTTAGTAAA GGCAGGAACT 
CTGTCCAAGG AGCAGTATTA CAAAGGGGTG CTTGAAGTTC TTGAAGTAGC AGAAGATATG 
GAAATAGATA TACCCCACAT CTGGCTGTAT TTAGCAGAGA TAATCAGCCC TGTGCTGCTA 
GAGGGGGGGA TTCCGATGGG GGAGCTGTTC AGAGAGCTGA CCAAACCTTT AGTGCTCATT 
GGCAAAGCTG GAGTCCTTCT GGCAGAAATC CTTGGATTAT TGTGCAAAGG CATGAGTCAT 
AAGAAGGCTG GAGCCCTGTG GAAAGAATCT GGGCTAAGCT GGAAGGAATT CTTGTCCAAG 
GACCAGGATA TTAACAAGTT TATAACTGAC AAGAAACTCG AATACACAAC AGGAGAGGAG 
ACTGAAACAC CCAGTAAGAA AGACCTCACC CCTGCTGAAC TTGATAAAAT TTTGACAAAA 
CTTTTGAAAG AGAACAGCAG TAACCAGAAG ATATTTGACT GGATTGAAGC CAACTTAAAT 
GACCAGCAAA CTTCATCCAA CACCTTTGTT CGAACTTTAA TGAGTACTGT GTGCCACTCT 
GCTATTATTT TTGAGAATCC TTGTAGGGTT GATGTTAATA TTATCCATAC TAGAGCCAAA 
TTTCTTCAGA AATATTTAAG TGACGAGGCC AAGGAACTAC AGGCTCTCTA TGCTCTACAG 
GCACTTGTTG TAAAGCTTGA CCAGCCCCCA AATCTCCTAC GGATGTTTTT TGATGCTCTC 
TATGATGAGG ATGTGATAAA AGAGGATCCA TTCTACAAGT GGGAGTCCAG CAAAGACCCA 
GCTGAACAGC AAGGAAAAGG TGTTGCTCTT AAATCTGTCA CTGCCTTCTT CACATGGCTA 
CGGGAAGCAG AGGATGAGTC TGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_004914400.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914457.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X6, mRNA.

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CloneID	OXa10205
Clone ID Related Accession (Same CDS sequence)	XM_004914401.4 , XM_004914400.3 , XM_004914401.3 , XM_012963378.2 , XM_012963378.3 , XM_004914400.4
Accession Version	XM_004914400.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914400.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC 
ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG 
TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA 
TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG 
ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA 
GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG 
AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC 
AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG 
CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT 
CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC 
AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA 
AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG 
CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC 
CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG 
GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG 
CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT 
TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT 
CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA 
CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT 
GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC 
TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA 
CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC 
ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA 
GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC 
TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA 
CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC 
TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC 
CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT 
GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC 
CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT 
GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG 
GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG 
CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA 
CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT 
ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA 
CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA 
TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG 
AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA 
ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT 
TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC 
AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG 
TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG 
AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG 
TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG 
GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC 
ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC 
CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG 
GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA 
ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG 
CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC 
CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT 
CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA 
CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC 
AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT 
TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG 
GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA 
AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT 
CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT 
ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA 
GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT 
ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT 
AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT 
ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT 
ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC 
AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC 
TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG 
GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG 
GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG 
AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT 
ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA 
GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT 
AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC 
ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT 
TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT 
GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC 
CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA 
GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT 
GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT 
GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914457.1
CDS	215..5023
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_004914400.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914400.3

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC 
ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG 
TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA 
TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG 
ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA 
GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG 
AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC 
AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG 
CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT 
CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC 
AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA 
AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG 
CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC 
CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG 
GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG 
CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT 
TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT 
CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA 
CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT 
GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC 
TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA 
CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC 
ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA 
GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC 
TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA 
CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC 
TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC 
CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT 
GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC 
CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT 
GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG 
GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG 
CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA 
CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT 
ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA 
CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA 
TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG 
AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA 
ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT 
TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC 
AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG 
TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG 
AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG 
TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG 
GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC 
ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC 
CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG 
GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA 
ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG 
CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC 
CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT 
CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA 
CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC 
AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT 
TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG 
GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA 
AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT 
CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT 
ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA 
GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT 
ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT 
AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT 
ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT 
ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC 
AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC 
TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG 
GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG 
GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG 
AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT 
ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA 
GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT 
AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC 
ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT 
TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT 
GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC 
CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA 
GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT 
GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT 
GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_004914401.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914458.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa10205
Clone ID Related Accession (Same CDS sequence)	XM_004914401.4 , XM_004914400.3 , XM_004914401.3 , XM_012963378.2 , XM_012963378.3 , XM_004914400.4
Accession Version	XM_004914401.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914401.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC 
ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG 
TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA 
TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG 
ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA 
GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG 
AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC 
AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG 
CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT 
CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC 
AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA 
AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG 
CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC 
CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG 
GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG 
CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT 
TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT 
CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA 
CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT 
GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC 
TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA 
CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC 
ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA 
GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC 
TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA 
CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC 
TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC 
CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT 
GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC 
CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT 
GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG 
GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG 
CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA 
CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT 
ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA 
CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA 
TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG 
AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA 
ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT 
TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC 
AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG 
TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG 
AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG 
TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG 
GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC 
ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC 
CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG 
GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA 
ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG 
CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC 
CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT 
CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA 
CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC 
AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT 
TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG 
GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA 
AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT 
CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT 
ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA 
GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT 
ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT 
AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT 
ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT 
ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC 
AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC 
TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG 
GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG 
GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG 
AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT 
ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA 
GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT 
AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC 
ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT 
TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT 
GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC 
CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA 
GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT 
GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT 
GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914458.1
CDS	195..5003
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_004914401.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914401.3

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC 
ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG 
TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA 
TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG 
ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA 
GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG 
AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC 
AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG 
CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT 
CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC 
AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA 
AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG 
CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC 
CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG 
GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG 
CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT 
TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT 
CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA 
CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT 
GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC 
TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA 
CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC 
ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA 
GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC 
TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA 
CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC 
TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC 
CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT 
GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC 
CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT 
GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG 
GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG 
CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA 
CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT 
ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA 
CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA 
TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG 
AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA 
ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT 
TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC 
AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG 
TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG 
AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG 
TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG 
GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC 
ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC 
CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG 
GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA 
ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG 
CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC 
CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT 
CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA 
CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC 
AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT 
TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG 
GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA 
AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT 
CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT 
ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA 
GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT 
ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT 
AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT 
ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT 
ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC 
AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC 
TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG 
GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG 
GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG 
AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT 
ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA 
GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT 
AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC 
ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT 
TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT 
GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC 
CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA 
GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT 
GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT 
GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_012963378.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818832.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X8, mRNA.

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CloneID	OXa10205
Clone ID Related Accession (Same CDS sequence)	XM_004914401.4 , XM_004914400.3 , XM_004914401.3 , XM_012963378.2 , XM_012963378.3 , XM_004914400.4
Accession Version	XM_012963378.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC 
ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG 
TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA 
TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG 
ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA 
GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG 
AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC 
AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG 
CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT 
CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC 
AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA 
AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG 
CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC 
CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG 
GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG 
CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT 
TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT 
CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA 
CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT 
GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC 
TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA 
CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC 
ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA 
GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC 
TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA 
CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC 
TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC 
CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT 
GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC 
CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT 
GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG 
GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG 
CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA 
CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT 
ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA 
CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA 
TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG 
AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA 
ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT 
TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC 
AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG 
TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG 
AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG 
TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG 
GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC 
ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC 
CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG 
GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA 
ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG 
CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC 
CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT 
CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA 
CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC 
AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT 
TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG 
GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA 
AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT 
CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT 
ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA 
GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT 
ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT 
AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT 
ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT 
ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC 
AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC 
TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG 
GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG 
GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG 
AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT 
ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA 
GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT 
AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC 
ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT 
TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT 
GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC 
CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA 
GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT 
GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT 
GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818832.1
CDS	343..5151
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQHYYQNRTQPPTSATRVQSGSSARAVTPAHVYQPGSQVMMIPSQISYPSQGYYIQGQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_012963378.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963378.2

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG CATTATTATC AAAATAGGAC ACAGCCTCCC 
ACTAGTGCCA CCAGAGTGCA GAGTGGTTCT TCTGCTCGAG CAGTTACTCC AGCCCATGTG 
TATCAACCAG GCTCCCAAGT TATGATGATT CCTTCACAGA TTTCATACCC TTCCCAGGGA 
TACTACATTC AGGGACAGGG ACGCTCATAT GTGGTGCCCA CAAACCAGTA CCCTGTGCAG 
ACTGGGGCTC CAGGTTTCTT CCCCAGTGCA AGTCCCTCAG AATATGGCAC ATATGCTGGA 
GCATATTACC CTGCACAACA GACGTTCCCT CCTGGAGTGC CTGGAGCACA AGTTATGATG 
AATCAGCCAC AGGTGATCCC TCCTAAACGG GAGCGCAAAA CGATAAGAAT TAGAGACCCC 
AACCAAGGAG GCAAAGATAT CACAGAAGAA ATTCTCTCAG GAGGAAGAAC ATCCTCTACG 
CCAACGCCTC CACAGTCAAC TGTGGGAAGT TCTGAAATTC AAGCTAATGG AGAAAGCGTT 
CATCATGTTA CCGTTGTTGT GCGCCCAGAT GATGGACCCA AGGCCACCCA AGTGCTGATC 
AAAGCCTCAG AGAGCCCTTC CCCTCCTCTG CCTCTCTCTG AAGCTGAGCC AAGCATAGCA 
AATGTGTGTG ATATTTCAAA AGATAACACT ATCTCTGAGT TGACTTCTCA TATAGTAGAG 
CCCCTAAGTT CAGAAGAGAC AACCATTGAT ACTTCTACCT CTCAGGTGGA TCTCAAACAC 
CAGCCAGTGG CTGCTATGGC AGAGCATGAA ACTCTTATAG AGGAGGCATC AATACAGGAG 
GTGGCCTTTG CAGAGGCACC CATCGCTCAA TCAGAGATAC CAAAATCTCT TGCCTCCCAG 
CCTCTAGCAG AAATCTCTAC CCCTGAACAG ATTTCTAAAC TTGACGCACA AGTGCCATCT 
TTGCAACAAG AGGAGTTACT TACAGAAACT GTTGAACTTA ATTTAAGCAA TCAGGCTCTT 
CCTTCACAAA TTTCTGAGCC TGAACCTTTT GAACCTGAGG CTTCAGCTTC AGATCCTCTA 
CATGAGGAGC CCACAGAGCC CACAGTTTCA ACAGAAGAAC CACAAGCTAA TGGACTGTCT 
GAGGAGACAC CAGTATTACC TGTACAGCCA CAACCAACAT CTGTTTGCCA GACTGAGCCC 
TCCCTAGAAT CCCCCATCGC TCAGCCTGAG GAGCTGCTTC TAACAAATGG AGGTGGACCA 
CCTGGCCTAC AAGAAAATGA TGCACCTGAT TCACAGGTTG AAGCTCATGT TAGCCCAATC 
ACTGAGCCTG AGGACCAACC TGTGACAGTC TCTGATAACG TGCTTGTGGA AGAGGATGTA 
GAAAAAACAT TGCCTGCGGA TACGGAAGTC GTGGCTTCAA CCCAAGTTGT AAAGCAGAGC 
TCCGAGAGTC AGTCTCAGGT TGCTGTTGCT GCACCCAAGA AAAAGCGAAA AATGAAGGAA 
CTAAACAAGA AAGAGGCAGT GGGAGATCTC CTTGATGCGT TCAAAGAGGC TGGTGATGGC 
TCTTCTGATG TGGATTCTTC CTCTAAGCCT ACCAGTGCTG CACCTGAACC AGATCAGAGC 
CCTTGTGCTC CTCCTGAGGA ACAGGATGAA ACTTGGGAAG AGAAGGAAGA TAAACTGGAT 
GCAGAGAATA TTAAACCAGG AGAACATAAA TATAAATACA AAGAAGAGCA ATGGAAACCC 
CTAAACCCGG AGGAAAAGAA GAGTTATGAT CGCGAGTTTC TGCTAGGGTT CCAGTTTATT 
GTGGCCAGTA TGCAGAAACC TGCGGGTCTT CCTCAAATTA CTGATGTGGT GCTGGATAAG 
GCAAACAAAA CTCCATTGCG ACCATTGGAT CCTACACGTC TGACGGGAAT GAACTGTGGG 
CCAGATTTTA CACCTTCATT TGCCAACCTT GGTCGGCCTG CTGTGGGTAA CCGTGGACCA 
CCAGCTGGCA TGGGTCCGAG ACGGTCCCAG CAGGGTCCAC GAAAAGAGCC TCGTAAAATT 
ATTCAAACCG TATCATTAAA TGAGAATGTA CAACTAAACA AAGCTGAAAA GGCATGGAAA 
CCGATAAACA AGAGAGCTGG CGATGAGGAG GACCCAGAAA ATATTAAGAC ACAGGACCTA 
TTCCGAAGGG TTCGCAGTAT CTTGAATAAG CTGACCCCTC AGATGTTCCA GCAGCTGATG 
AAGCAGGTCA CTGACCTTAC TATTGATACC GAAGATCGTC TTAAGGGTGT CATTGACCTA 
ATTTTTGAAA AAGCCATTTC AGAACCCAAT TTCTCTGTTG CATATGCCAA CATGTGTCGT 
TGCCTTATGG GGCTTAAAGT TCCAACCACA GACAAGCCAG GGGTTACAGT CAACTTCCGC 
AAGTTGTTAC TGAATCGGTG CCAGAAGGAG TTTGAGAAGG ATAAGGATGA TGATGAAGTG 
TTTGAGAAGA AACAAAAAGA AATGGATGCT GCTGCTTCGC CGGAGGAAAA AGCACGTTTG 
AATGATGAAC TGGTCGAGGC CCGTGACAGT GCACGACGGC GATCCTTGGG GAACATTAAG 
TTCATTGGAG AACTGTTTAA GTTAAAAATG CTGACTGAAG CTATAATGCA CGATTGTGTG 
GTGAAGCTAT TAAAAAATCA TGATGAGGAA TCTCTGGAGT GCCTCTGCCG ACTGCTGTCC 
ACCATTGGCA AGGACCTGGA TTTTGAGAGA GCTAAGCCAC GTATGGACCA GTACTTCAAC 
CAGATGGATA AAATCATTAA GGAGCGGAAG ACATCTTCAC GAATTAGATT CATGCTGCAG 
GATGTGATTG ATTTGCGTCT GTGTAATTGG GTGCCTCGTC GTGCAGATCA GGGCCCTAAA 
ACTATAGATC AGATTCATAA AGAAGCAGAA ATGGAAAAGC AGAGGGAGCA GGTTAAGGTG 
CAACAACTGA TGTCTAAACA AGACAAGCGG CGAGAGCCCC CAGGACGACC CACTGGAAGC 
CGGGGTAACC AGCCTGCAGA TGATGGCTGG AATACAGTGC CCATCAGTAA GGGCAGCCGT 
CCTATTGACA CCACTAGGAT CACAAAGATA ACAAAGCTTG GTGCTATTGA TTCAAATCAA 
CTGCTTGCCC CAGGAGGACG TCTGAGTTGG GGTAAAGGAA GCAGCGGAGG AACTGGAGCC 
AAACCAGCAG AAACAGCTCC AGAATCTAGT CGGCCTGCGA CCAGCACCTT AAACAGGTTT 
TCTGCCTTGC AGCAGTCTTC ATCTGCTGAG AGTCAAGATG CTAGACGGCC CTTGCATCGG 
GGAAGTTCTA GTCGGGAACG TTCTGAAAAA GATCGTGGAG ACAGATTTGA TAGAGGTGAA 
AGGTTTGATA AGGGAGAACT GTTTGATAGA TCTGACCGTT CAGATCGAGC TCGTCCTCTT 
CTTACAAAGA GAAGCTATAG CAAGGAAACT GAGGACCGTA GCCGAGAAAG AGAGGCCCAT 
ATACCACAGG AGGCTGTGAG GAAGGTGGCA AGTATGACTG AGCAGCGGGA CCGCAGCAAA 
GAAAGTGTGA AAAAAGAAAT TCCAGCACCG ACAGCAGTGG TTGCCTCATC AAAGCCCTCT 
ATGTCTGAGG ATGAGCTAGA GAAGAAATCT AAAGCCATCA TAGAAGAATA TTTGCACATT 
AATGATATGA AGGAAGCTCT TCAGTGTGTA GTGGAGCTTA ATAGTTCAAC GCTGCTTTTT 
ATCTTTGTGC GGAATGGGAT TGAGTCAACA CTAGAGAGAA GCACTATTGC CAGAGAGCAT 
ATGGGTCTCC TTTTGTACCA GTTAGTAAAG GCAGGAACTC TGTCCAAGGA GCAGTATTAC 
AAAGGGGTGC TTGAAGTTCT TGAAGTAGCA GAAGATATGG AAATAGATAT ACCCCACATC 
TGGCTGTATT TAGCAGAGAT AATCAGCCCT GTGCTGCTAG AGGGGGGGAT TCCGATGGGG 
GAGCTGTTCA GAGAGCTGAC CAAACCTTTA GTGCTCATTG GCAAAGCTGG AGTCCTTCTG 
GCAGAAATCC TTGGATTATT GTGCAAAGGC ATGAGTCATA AGAAGGCTGG AGCCCTGTGG 
AAAGAATCTG GGCTAAGCTG GAAGGAATTC TTGTCCAAGG ACCAGGATAT TAACAAGTTT 
ATAACTGACA AGAAACTCGA ATACACAACA GGAGAGGAGA CTGAAACACC CAGTAAGAAA 
GACCTCACCC CTGCTGAACT TGATAAAATT TTGACAAAAC TTTTGAAAGA GAACAGCAGT 
AACCAGAAGA TATTTGACTG GATTGAAGCC AACTTAAATG ACCAGCAAAC TTCATCCAAC 
ACCTTTGTTC GAACTTTAAT GAGTACTGTG TGCCACTCTG CTATTATTTT TGAGAATCCT 
TGTAGGGTTG ATGTTAATAT TATCCATACT AGAGCCAAAT TTCTTCAGAA ATATTTAAGT 
GACGAGGCCA AGGAACTACA GGCTCTCTAT GCTCTACAGG CACTTGTTGT AAAGCTTGAC 
CAGCCCCCAA ATCTCCTACG GATGTTTTTT GATGCTCTCT ATGATGAGGA TGTGATAAAA 
GAGGATCCAT TCTACAAGTG GGAGTCCAGC AAAGACCCAG CTGAACAGCA AGGAAAAGGT 
GTTGCTCTTA AATCTGTCAC TGCCTTCTTC ACATGGCTAC GGGAAGCAGA GGATGAGTCT 
GATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

eif4g1 ( XM_004914402.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004914459.1 Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X9, mRNA.

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CloneID	OXa10206
Clone ID Related Accession (Same CDS sequence)	XM_004914402.3 , XM_004914402.4
Accession Version	XM_004914402.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	eukaryotic translation initiation factor 4 gamma 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004914402.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GGACGCTCAT ATGTGGTGCC CACAAACCAG 
TACCCTGTGC AGACTGGGGC TCCAGGTTTC TTCCCCAGTG CAAGTCCCTC AGAATATGGC 
ACATATGCTG GAGCATATTA CCCTGCACAA CAGACGTTCC CTCCTGGAGT GCCTGGAGCA 
CAAGTTATGA TGAATCAGCC ACAGGTGATC CCTCCTAAAC GGGAGCGCAA AACGATAAGA 
ATTAGAGACC CCAACCAAGG AGGCAAAGAT ATCACAGAAG AAATTCTCTC AGGAGGAAGA 
ACATCCTCTA CGCCAACGCC TCCACAGTCA ACTGTGGGAA GTTCTGAAAT TCAAGCTAAT 
GGAGAAAGCG TTCATCATGT TACCGTTGTT GTGCGCCCAG ATGATGGACC CAAGGCCACC 
CAAGTGCTGA TCAAAGCCTC AGAGAGCCCT TCCCCTCCTC TGCCTCTCTC TGAAGCTGAG 
CCAAGCATAG CAAATGTGTG TGATATTTCA AAAGATAACA CTATCTCTGA GTTGACTTCT 
CATATAGTAG AGCCCCTAAG TTCAGAAGAG ACAACCATTG ATACTTCTAC CTCTCAGGTG 
GATCTCAAAC ACCAGCCAGT GGCTGCTATG GCAGAGCATG AAACTCTTAT AGAGGAGGCA 
TCAATACAGG AGGTGGCCTT TGCAGAGGCA CCCATCGCTC AATCAGAGAT ACCAAAATCT 
CTTGCCTCCC AGCCTCTAGC AGAAATCTCT ACCCCTGAAC AGATTTCTAA ACTTGACGCA 
CAAGTGCCAT CTTTGCAACA AGAGGAGTTA CTTACAGAAA CTGTTGAACT TAATTTAAGC 
AATCAGGCTC TTCCTTCACA AATTTCTGAG CCTGAACCTT TTGAACCTGA GGCTTCAGCT 
TCAGATCCTC TACATGAGGA GCCCACAGAG CCCACAGTTT CAACAGAAGA ACCACAAGCT 
AATGGACTGT CTGAGGAGAC ACCAGTATTA CCTGTACAGC CACAACCAAC ATCTGTTTGC 
CAGACTGAGC CCTCCCTAGA ATCCCCCATC GCTCAGCCTG AGGAGCTGCT TCTAACAAAT 
GGAGGTGGAC CACCTGGCCT ACAAGAAAAT GATGCACCTG ATTCACAGGT TGAAGCTCAT 
GTTAGCCCAA TCACTGAGCC TGAGGACCAA CCTGTGACAG TCTCTGATAA CGTGCTTGTG 
GAAGAGGATG TAGAAAAAAC ATTGCCTGCG GATACGGAAG TCGTGGCTTC AACCCAAGTT 
GTAAAGCAGA GCTCCGAGAG TCAGTCTCAG GTTGCTGTTG CTGCACCCAA GAAAAAGCGA 
AAAATGAAGG AACTAAACAA GAAAGAGGCA GTGGGAGATC TCCTTGATGC GTTCAAAGAG 
GCTGGTGATG GCTCTTCTGA TGTGGATTCT TCCTCTAAGC CTACCAGTGC TGCACCTGAA 
CCAGATCAGA GCCCTTGTGC TCCTCCTGAG GAACAGGATG AAACTTGGGA AGAGAAGGAA 
GATAAACTGG ATGCAGAGAA TATTAAACCA GGAGAACATA AATATAAATA CAAAGAAGAG 
CAATGGAAAC CCCTAAACCC GGAGGAAAAG AAGAGTTATG ATCGCGAGTT TCTGCTAGGG 
TTCCAGTTTA TTGTGGCCAG TATGCAGAAA CCTGCGGGTC TTCCTCAAAT TACTGATGTG 
GTGCTGGATA AGGCAAACAA AACTCCATTG CGACCATTGG ATCCTACACG TCTGACGGGA 
ATGAACTGTG GGCCAGATTT TACACCTTCA TTTGCCAACC TTGGTCGGCC TGCTGTGGGT 
AACCGTGGAC CACCAGCTGG CATGGGTCCG AGACGGTCCC AGCAGGGTCC ACGAAAAGAG 
CCTCGTAAAA TTATTCAAAC CGTATCATTA AATGAGAATG TACAACTAAA CAAAGCTGAA 
AAGGCATGGA AACCGATAAA CAAGAGAGCT GGCGATGAGG AGGACCCAGA AAATATTAAG 
ACACAGGACC TATTCCGAAG GGTTCGCAGT ATCTTGAATA AGCTGACCCC TCAGATGTTC 
CAGCAGCTGA TGAAGCAGGT CACTGACCTT ACTATTGATA CCGAAGATCG TCTTAAGGGT 
GTCATTGACC TAATTTTTGA AAAAGCCATT TCAGAACCCA ATTTCTCTGT TGCATATGCC 
AACATGTGTC GTTGCCTTAT GGGGCTTAAA GTTCCAACCA CAGACAAGCC AGGGGTTACA 
GTCAACTTCC GCAAGTTGTT ACTGAATCGG TGCCAGAAGG AGTTTGAGAA GGATAAGGAT 
GATGATGAAG TGTTTGAGAA GAAACAAAAA GAAATGGATG CTGCTGCTTC GCCGGAGGAA 
AAAGCACGTT TGAATGATGA ACTGGTCGAG GCCCGTGACA GTGCACGACG GCGATCCTTG 
GGGAACATTA AGTTCATTGG AGAACTGTTT AAGTTAAAAA TGCTGACTGA AGCTATAATG 
CACGATTGTG TGGTGAAGCT ATTAAAAAAT CATGATGAGG AATCTCTGGA GTGCCTCTGC 
CGACTGCTGT CCACCATTGG CAAGGACCTG GATTTTGAGA GAGCTAAGCC ACGTATGGAC 
CAGTACTTCA ACCAGATGGA TAAAATCATT AAGGAGCGGA AGACATCTTC ACGAATTAGA 
TTCATGCTGC AGGATGTGAT TGATTTGCGT CTGTGTAATT GGGTGCCTCG TCGTGCAGAT 
CAGGGCCCTA AAACTATAGA TCAGATTCAT AAAGAAGCAG AAATGGAAAA GCAGAGGGAG 
CAGGTTAAGG TGCAACAACT GATGTCTAAA CAAGACAAGC GGCGAGAGCC CCCAGGACGA 
CCCACTGGAA GCCGGGGTAA CCAGCCTGCA GATGATGGCT GGAATACAGT GCCCATCAGT 
AAGGGCAGCC GTCCTATTGA CACCACTAGG ATCACAAAGA TAACAAAGCT TGGTGCTATT 
GATTCAAATC AACTGCTTGC CCCAGGAGGA CGTCTGAGTT GGGGTAAAGG AAGCAGCGGA 
GGAACTGGAG CCAAACCAGC AGAAACAGCT CCAGAATCTA GTCGGCCTGC GACCAGCACC 
TTAAACAGGT TTTCTGCCTT GCAGCAGTCT TCATCTGCTG AGAGTCAAGA TGCTAGACGG 
CCCTTGCATC GGGGAAGTTC TAGTCGGGAA CGTTCTGAAA AAGATCGTGG AGACAGATTT 
GATAGAGGTG AAAGGTTTGA TAAGGGAGAA CTGTTTGATA GATCTGACCG TTCAGATCGA 
GCTCGTCCTC TTCTTACAAA GAGAAGCTAT AGCAAGGAAA CTGAGGACCG TAGCCGAGAA 
AGAGAGGCCC ATATACCACA GGAGGCTGTG AGGAAGGTGG CAAGTATGAC TGAGCAGCGG 
GACCGCAGCA AAGAAAGTGT GAAAAAAGAA ATTCCAGCAC CGACAGCAGT GGTTGCCTCA 
TCAAAGCCCT CTATGTCTGA GGATGAGCTA GAGAAGAAAT CTAAAGCCAT CATAGAAGAA 
TATTTGCACA TTAATGATAT GAAGGAAGCT CTTCAGTGTG TAGTGGAGCT TAATAGTTCA 
ACGCTGCTTT TTATCTTTGT GCGGAATGGG ATTGAGTCAA CACTAGAGAG AAGCACTATT 
GCCAGAGAGC ATATGGGTCT CCTTTTGTAC CAGTTAGTAA AGGCAGGAAC TCTGTCCAAG 
GAGCAGTATT ACAAAGGGGT GCTTGAAGTT CTTGAAGTAG CAGAAGATAT GGAAATAGAT 
ATACCCCACA TCTGGCTGTA TTTAGCAGAG ATAATCAGCC CTGTGCTGCT AGAGGGGGGG 
ATTCCGATGG GGGAGCTGTT CAGAGAGCTG ACCAAACCTT TAGTGCTCAT TGGCAAAGCT 
GGAGTCCTTC TGGCAGAAAT CCTTGGATTA TTGTGCAAAG GCATGAGTCA TAAGAAGGCT 
GGAGCCCTGT GGAAAGAATC TGGGCTAAGC TGGAAGGAAT TCTTGTCCAA GGACCAGGAT 
ATTAACAAGT TTATAACTGA CAAGAAACTC GAATACACAA CAGGAGAGGA GACTGAAACA 
CCCAGTAAGA AAGACCTCAC CCCTGCTGAA CTTGATAAAA TTTTGACAAA ACTTTTGAAA 
GAGAACAGCA GTAACCAGAA GATATTTGAC TGGATTGAAG CCAACTTAAA TGACCAGCAA 
ACTTCATCCA ACACCTTTGT TCGAACTTTA ATGAGTACTG TGTGCCACTC TGCTATTATT 
TTTGAGAATC CTTGTAGGGT TGATGTTAAT ATTATCCATA CTAGAGCCAA ATTTCTTCAG 
AAATATTTAA GTGACGAGGC CAAGGAACTA CAGGCTCTCT ATGCTCTACA GGCACTTGTT 
GTAAAGCTTG ACCAGCCCCC AAATCTCCTA CGGATGTTTT TTGATGCTCT CTATGATGAG 
GATGTGATAA AAGAGGATCC ATTCTACAAG TGGGAGTCCA GCAAAGACCC AGCTGAACAG 
CAAGGAAAAG GTGTTGCTCT TAAATCTGTC ACTGCCTTCT TCACATGGCT ACGGGAAGCA 
GAGGATGAGT CTGATAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004914459.1
CDS	216..4856
Translation	MSKTTQTIGGPPTAPSPGLSQTAFPSAPAAPVVFGPPQPPQMNTQSQPRQGRSYVVPTNQYPVQTGAPGFFPSASPSEYGTYAGAYYPAQQTFPPGVPGAQVMMNQPQVIPPKRERKTIRIRDPNQGGKDITEEILSGGRTSSTPTPPQSTVGSSEIQANGESVHHVTVVVRPDDGPKATQVLIKASESPSPPLPLSEAEPSIANVCDISKDNTISELTSHIVEPLSSEETTIDTSTSQVDLKHQPVAAMAEHETLIEEASIQEVAFAEAPIAQSEIPKSLASQPLAEISTPEQISKLDAQVPSLQQEELLTETVELNLSNQALPSQISEPEPFEPEASASDPLHEEPTEPTVSTEEPQANGLSEETPVLPVQPQPTSVCQTEPSLESPIAQPEELLLTNGGGPPGLQENDAPDSQVEAHVSPITEPEDQPVTVSDNVLVEEDVEKTLPADTEVVASTQVVKQSSESQSQVAVAAPKKKRKMKELNKKEAVGDLLDAFKEAGDGSSDVDSSSKPTSAAPEPDQSPCAPPEEQDETWEEKEDKLDAENIKPGEHKYKYKEEQWKPLNPEEKKSYDREFLLGFQFIVASMQKPAGLPQITDVVLDKANKTPLRPLDPTRLTGMNCGPDFTPSFANLGRPAVGNRGPPAGMGPRRSQQGPRKEPRKIIQTVSLNENVQLNKAEKAWKPINKRAGDEEDPENIKTQDLFRRVRSILNKLTPQMFQQLMKQVTDLTIDTEDRLKGVIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTTDKPGVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDAAASPEEKARLNDELVEARDSARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLSTIGKDLDFERAKPRMDQYFNQMDKIIKERKTSSRIRFMLQDVIDLRLCNWVPRRADQGPKTIDQIHKEAEMEKQREQVKVQQLMSKQDKRREPPGRPTGSRGNQPADDGWNTVPISKGSRPIDTTRITKITKLGAIDSNQLLAPGGRLSWGKGSSGGTGAKPAETAPESSRPATSTLNRFSALQQSSSAESQDARRPLHRGSSSRERSEKDRGDRFDRGERFDKGELFDRSDRSDRARPLLTKRSYSKETEDRSREREAHIPQEAVRKVASMTEQRDRSKESVKKEIPAPTAVVASSKPSMSEDELEKKSKAIIEEYLHINDMKEALQCVVELNSSTLLFIFVRNGIESTLERSTIAREHMGLLLYQLVKAGTLSKEQYYKGVLEVLEVAEDMEIDIPHIWLYLAEIISPVLLEGGIPMGELFRELTKPLVLIGKAGVLLAEILGLLCKGMSHKKAGALWKESGLSWKEFLSKDQDINKFITDKKLEYTTGEETETPSKKDLTPAELDKILTKLLKENSSNQKIFDWIEANLNDQQTSSNTFVRTLMSTVCHSAIIFENPCRVDVNIIHTRAKFLQKYLSDEAKELQALYALQALVVKLDQPPNLLRMFFDALYDEDVIKEDPFYKWESSKDPAEQQGKGVALKSVTAFFTWLREAEDESDN

Target ORF information:

RefSeq Version	XM_004914402.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis eukaryotic translation initiation factor 4 gamma, 1 (eif4g1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004914402.3

ORF Insert Sequence:

ATGAGTAAAA CCACACAAAC AATAGGAGGA CCCCCTACAG CCCCTTCCCC TGGACTCTCA 
CAAACAGCTT TCCCTTCTGC TCCTGCGGCC CCTGTTGTGT TCGGGCCCCC ACAGCCCCCA 
CAAATGAATA CACAATCTCA GCCTCGACAG GGACGCTCAT ATGTGGTGCC CACAAACCAG 
TACCCTGTGC AGACTGGGGC TCCAGGTTTC TTCCCCAGTG CAAGTCCCTC AGAATATGGC 
ACATATGCTG GAGCATATTA CCCTGCACAA CAGACGTTCC CTCCTGGAGT GCCTGGAGCA 
CAAGTTATGA TGAATCAGCC ACAGGTGATC CCTCCTAAAC GGGAGCGCAA AACGATAAGA 
ATTAGAGACC CCAACCAAGG AGGCAAAGAT ATCACAGAAG AAATTCTCTC AGGAGGAAGA 
ACATCCTCTA CGCCAACGCC TCCACAGTCA ACTGTGGGAA GTTCTGAAAT TCAAGCTAAT 
GGAGAAAGCG TTCATCATGT TACCGTTGTT GTGCGCCCAG ATGATGGACC CAAGGCCACC 
CAAGTGCTGA TCAAAGCCTC AGAGAGCCCT TCCCCTCCTC TGCCTCTCTC TGAAGCTGAG 
CCAAGCATAG CAAATGTGTG TGATATTTCA AAAGATAACA CTATCTCTGA GTTGACTTCT 
CATATAGTAG AGCCCCTAAG TTCAGAAGAG ACAACCATTG ATACTTCTAC CTCTCAGGTG 
GATCTCAAAC ACCAGCCAGT GGCTGCTATG GCAGAGCATG AAACTCTTAT AGAGGAGGCA 
TCAATACAGG AGGTGGCCTT TGCAGAGGCA CCCATCGCTC AATCAGAGAT ACCAAAATCT 
CTTGCCTCCC AGCCTCTAGC AGAAATCTCT ACCCCTGAAC AGATTTCTAA ACTTGACGCA 
CAAGTGCCAT CTTTGCAACA AGAGGAGTTA CTTACAGAAA CTGTTGAACT TAATTTAAGC 
AATCAGGCTC TTCCTTCACA AATTTCTGAG CCTGAACCTT TTGAACCTGA GGCTTCAGCT 
TCAGATCCTC TACATGAGGA GCCCACAGAG CCCACAGTTT CAACAGAAGA ACCACAAGCT 
AATGGACTGT CTGAGGAGAC ACCAGTATTA CCTGTACAGC CACAACCAAC ATCTGTTTGC 
CAGACTGAGC CCTCCCTAGA ATCCCCCATC GCTCAGCCTG AGGAGCTGCT TCTAACAAAT 
GGAGGTGGAC CACCTGGCCT ACAAGAAAAT GATGCACCTG ATTCACAGGT TGAAGCTCAT 
GTTAGCCCAA TCACTGAGCC TGAGGACCAA CCTGTGACAG TCTCTGATAA CGTGCTTGTG 
GAAGAGGATG TAGAAAAAAC ATTGCCTGCG GATACGGAAG TCGTGGCTTC AACCCAAGTT 
GTAAAGCAGA GCTCCGAGAG TCAGTCTCAG GTTGCTGTTG CTGCACCCAA GAAAAAGCGA 
AAAATGAAGG AACTAAACAA GAAAGAGGCA GTGGGAGATC TCCTTGATGC GTTCAAAGAG 
GCTGGTGATG GCTCTTCTGA TGTGGATTCT TCCTCTAAGC CTACCAGTGC TGCACCTGAA 
CCAGATCAGA GCCCTTGTGC TCCTCCTGAG GAACAGGATG AAACTTGGGA AGAGAAGGAA 
GATAAACTGG ATGCAGAGAA TATTAAACCA GGAGAACATA AATATAAATA CAAAGAAGAG 
CAATGGAAAC CCCTAAACCC GGAGGAAAAG AAGAGTTATG ATCGCGAGTT TCTGCTAGGG 
TTCCAGTTTA TTGTGGCCAG TATGCAGAAA CCTGCGGGTC TTCCTCAAAT TACTGATGTG 
GTGCTGGATA AGGCAAACAA AACTCCATTG CGACCATTGG ATCCTACACG TCTGACGGGA 
ATGAACTGTG GGCCAGATTT TACACCTTCA TTTGCCAACC TTGGTCGGCC TGCTGTGGGT 
AACCGTGGAC CACCAGCTGG CATGGGTCCG AGACGGTCCC AGCAGGGTCC ACGAAAAGAG 
CCTCGTAAAA TTATTCAAAC CGTATCATTA AATGAGAATG TACAACTAAA CAAAGCTGAA 
AAGGCATGGA AACCGATAAA CAAGAGAGCT GGCGATGAGG AGGACCCAGA AAATATTAAG 
ACACAGGACC TATTCCGAAG GGTTCGCAGT ATCTTGAATA AGCTGACCCC TCAGATGTTC 
CAGCAGCTGA TGAAGCAGGT CACTGACCTT ACTATTGATA CCGAAGATCG TCTTAAGGGT 
GTCATTGACC TAATTTTTGA AAAAGCCATT TCAGAACCCA ATTTCTCTGT TGCATATGCC 
AACATGTGTC GTTGCCTTAT GGGGCTTAAA GTTCCAACCA CAGACAAGCC AGGGGTTACA 
GTCAACTTCC GCAAGTTGTT ACTGAATCGG TGCCAGAAGG AGTTTGAGAA GGATAAGGAT 
GATGATGAAG TGTTTGAGAA GAAACAAAAA GAAATGGATG CTGCTGCTTC GCCGGAGGAA 
AAAGCACGTT TGAATGATGA ACTGGTCGAG GCCCGTGACA GTGCACGACG GCGATCCTTG 
GGGAACATTA AGTTCATTGG AGAACTGTTT AAGTTAAAAA TGCTGACTGA AGCTATAATG 
CACGATTGTG TGGTGAAGCT ATTAAAAAAT CATGATGAGG AATCTCTGGA GTGCCTCTGC 
CGACTGCTGT CCACCATTGG CAAGGACCTG GATTTTGAGA GAGCTAAGCC ACGTATGGAC 
CAGTACTTCA ACCAGATGGA TAAAATCATT AAGGAGCGGA AGACATCTTC ACGAATTAGA 
TTCATGCTGC AGGATGTGAT TGATTTGCGT CTGTGTAATT GGGTGCCTCG TCGTGCAGAT 
CAGGGCCCTA AAACTATAGA TCAGATTCAT AAAGAAGCAG AAATGGAAAA GCAGAGGGAG 
CAGGTTAAGG TGCAACAACT GATGTCTAAA CAAGACAAGC GGCGAGAGCC CCCAGGACGA 
CCCACTGGAA GCCGGGGTAA CCAGCCTGCA GATGATGGCT GGAATACAGT GCCCATCAGT 
AAGGGCAGCC GTCCTATTGA CACCACTAGG ATCACAAAGA TAACAAAGCT TGGTGCTATT 
GATTCAAATC AACTGCTTGC CCCAGGAGGA CGTCTGAGTT GGGGTAAAGG AAGCAGCGGA 
GGAACTGGAG CCAAACCAGC AGAAACAGCT CCAGAATCTA GTCGGCCTGC GACCAGCACC 
TTAAACAGGT TTTCTGCCTT GCAGCAGTCT TCATCTGCTG AGAGTCAAGA TGCTAGACGG 
CCCTTGCATC GGGGAAGTTC TAGTCGGGAA CGTTCTGAAA AAGATCGTGG AGACAGATTT 
GATAGAGGTG AAAGGTTTGA TAAGGGAGAA CTGTTTGATA GATCTGACCG TTCAGATCGA 
GCTCGTCCTC TTCTTACAAA GAGAAGCTAT AGCAAGGAAA CTGAGGACCG TAGCCGAGAA 
AGAGAGGCCC ATATACCACA GGAGGCTGTG AGGAAGGTGG CAAGTATGAC TGAGCAGCGG 
GACCGCAGCA AAGAAAGTGT GAAAAAAGAA ATTCCAGCAC CGACAGCAGT GGTTGCCTCA 
TCAAAGCCCT CTATGTCTGA GGATGAGCTA GAGAAGAAAT CTAAAGCCAT CATAGAAGAA 
TATTTGCACA TTAATGATAT GAAGGAAGCT CTTCAGTGTG TAGTGGAGCT TAATAGTTCA 
ACGCTGCTTT TTATCTTTGT GCGGAATGGG ATTGAGTCAA CACTAGAGAG AAGCACTATT 
GCCAGAGAGC ATATGGGTCT CCTTTTGTAC CAGTTAGTAA AGGCAGGAAC TCTGTCCAAG 
GAGCAGTATT ACAAAGGGGT GCTTGAAGTT CTTGAAGTAG CAGAAGATAT GGAAATAGAT 
ATACCCCACA TCTGGCTGTA TTTAGCAGAG ATAATCAGCC CTGTGCTGCT AGAGGGGGGG 
ATTCCGATGG GGGAGCTGTT CAGAGAGCTG ACCAAACCTT TAGTGCTCAT TGGCAAAGCT 
GGAGTCCTTC TGGCAGAAAT CCTTGGATTA TTGTGCAAAG GCATGAGTCA TAAGAAGGCT 
GGAGCCCTGT GGAAAGAATC TGGGCTAAGC TGGAAGGAAT TCTTGTCCAA GGACCAGGAT 
ATTAACAAGT TTATAACTGA CAAGAAACTC GAATACACAA CAGGAGAGGA GACTGAAACA 
CCCAGTAAGA AAGACCTCAC CCCTGCTGAA CTTGATAAAA TTTTGACAAA ACTTTTGAAA 
GAGAACAGCA GTAACCAGAA GATATTTGAC TGGATTGAAG CCAACTTAAA TGACCAGCAA 
ACTTCATCCA ACACCTTTGT TCGAACTTTA ATGAGTACTG TGTGCCACTC TGCTATTATT 
TTTGAGAATC CTTGTAGGGT TGATGTTAAT ATTATCCATA CTAGAGCCAA ATTTCTTCAG 
AAATATTTAA GTGACGAGGC CAAGGAACTA CAGGCTCTCT ATGCTCTACA GGCACTTGTT 
GTAAAGCTTG ACCAGCCCCC AAATCTCCTA CGGATGTTTT TTGATGCTCT CTATGATGAG 
GATGTGATAA AAGAGGATCC ATTCTACAAG TGGGAGTCCA GCAAAGACCC AGCTGAACAG 
CAAGGAAAAG GTGTTGCTCT TAAATCTGTC ACTGCCTTCT TCACATGGCT ACGGGAAGCA 
GAGGATGAGT CTGATAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	eif4g1
Entrez Gene ID	100380114
Full Name	eukaryotic translation initiation factor 4 gamma, 1
Synonyms	eif4g
General protein information	Preferred Names eukaryotic translation initiation factor 4 gamma, 1 Names eukaryotic translation initiation factor 4 gamma 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

	EIF4G1	XP_002802932.1
Canis lupus familiaris (dog)	EIF4G1	XP_005640005.1
Bos taurus (cattle)	EIF4G1	XP_003585711.2
Mus musculus (house mouse)	Eif4g1	NP_666053.2
Homo sapiens (human)	EIF4G1	NP_886553.3
Rattus norvegicus (Norway rat)	Eif4g1	XP_006248643.1
Gallus gallus (chicken)	EIF4G1	XP_004943468.1
Xenopus tropicalis (tropical clawed frog)	eif4g3	XP_004914456.1
Pan troglodytes (chimpanzee)	EIF4G1	XP_003310194.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

eif4g1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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