med14 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	med14
Entrez Gene ID	100379915
Full Name	mediator complex subunit 14
General protein information	Preferred Names mediator complex subunit 14 Names mediator of RNA polymerase II transcription subunit 14
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002932793.4	XP_002932839.1	mediator of RNA polymerase II transcription subunit 14 isoform X1
XM_004911773.3	XP_004911830.1	mediator of RNA polymerase II transcription subunit 14 isoform X3
XM_004911774.4	XP_004911831.1	mediator of RNA polymerase II transcription subunit 14 isoform X4
XM_004911772.3	XP_004911829.1	mediator of RNA polymerase II transcription subunit 14 isoform X2
XM_004911774.3	XP_004911831.1	mediator of RNA polymerase II transcription subunit 14 isoform X4
XM_002932793.5	XP_002932839.1	mediator of RNA polymerase II transcription subunit 14 isoform X1
XM_004911773.4	XP_004911830.1	mediator of RNA polymerase II transcription subunit 14 isoform X3
XM_004911772.4	XP_004911829.1	mediator of RNA polymerase II transcription subunit 14 isoform X2

Pathways from BioSystems

Homologies

	MED14	XP_001085867.1
Gallus gallus (chicken)	MED14	XP_416776.2
Xenopus tropicalis (tropical clawed frog)	med14	XP_002932839.1
Homo sapiens (human)	MED14	NP_004220.2
Bos taurus (cattle)	MED14	NP_001193960.1
Mus musculus (house mouse)	Med14	NP_001041673.1
Rattus norvegicus (Norway rat)	Med14	NP_001178656.1
Danio rerio (zebrafish)	med14	NP_997930.2
Anopheles gambiae (African malaria mosquito)	MED14_ANOGA	XP_315707.4
Drosophila melanogaster (fruit fly)	MED14	NP_612041.2
Pan troglodytes (chimpanzee)	MED14	XP_003317475.2
Canis lupus familiaris (dog)	MED14	XP_538001.2

Gene Ontology

The following med14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the med14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa05804	XM_002932793.5 Latest version!	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa05805	XM_004911772.4 Latest version!	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa05806	XM_004911773.4 Latest version!	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa05807	XM_004911774.4 Latest version!	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OXa05807	XM_004911774.3	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa05804	XM_002932793.4	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa05806	XM_004911773.3	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa05805	XM_004911772.3	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

med14 ( XM_002932793.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932839.1 Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa05804
Clone ID Related Accession (Same CDS sequence)	XM_002932793.4 , XM_002932793.5
Accession Version	XM_002932793.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932793.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTGCACTA 
GACCCTAGTT CTCCGTACAC CATGATGTCA CCCAGCCAGC GTGCAGGAAA TTGGCCAGGG 
TCCCCTCAAG TATCTGGTCC TTCTCCAGCA GCTCGTATGC CTGGAATGTC ACCAGCAAAT 
CCATCTCTTC ATTCTCCTGT CCCTGACGCG TCTCACTCCC CTCGTGCTGG AACAAGTTCT 
CAGTCAATGC CCACAAATAT GCCTCCTCCG CGTAAGCTAC CTCAGCGATC TTGGGCCGCA 
TCGATTCCTA CAATACTCAC TCACAGTGCC TTGCATGTGC TTTTACTGCC TTCTCCTACA 
CCTGGATTAG CCCCAGCATT GGCAGGCAGC TACCTGTGTT CCCCTCTTGA AAGGTTCTTG 
GGATCTGTCA ATATGAGACG ACACCTGCAA AGGATCATTC AGCAGGAAAC GCTGCAGTTA 
ATAAATTCCA ATGAACCAGG GGTGATAATG TTCAAAACAG ATGTATTAAA GTGCCGTGTG 
GCTCTTAACC CAACTACCAA CCAAACTTTG CAACTAAAAG TTACACCTGA GAATGCTGGA 
CAGTGGAAGC CAGAAGAGCT TCAAGTTTTA GAGAGGTTCT TTGAGACCAG GGTTGCAGGA 
CCTCCATTCA AGGCAAATAC TTTGATAGCT TTCACCAAGT TATTAGGAGC ACCTGCGCAT 
ATCCTCAGAG ACTGTGTGCA TATAATGAAG CTAGAACTGT TTCCAGATCA GACAACCCAA 
CTGAAATGGA ATGTGCAGTT CTGCCTAACC ATCCCACCTA GTGCACCACC AATAGCTCCA 
TCGGGAACTC CTGCTGTAGT ACTGAAATCT AAAATGTTGT TTTTTCTTCA GCTAACTCAA 
AAGACTTCAG CTCCAACAGA TGCTTTCAGT ATTATTGTTC CTATTATTTA TGATATGGCA 
TCAGGAACAA CCCAGCAAGC AGAAATCCCT CGGCAGCAGA ACTCTTCAGT AACGGCTCCC 
TTAACAGTTA GCAATATTCT GAAAAGGTTT GCTGAGATGA ATCCACCACG GCCAGGTGAA 
TGTACAATAT TTGCTGCAGT ACGAGATTTA ATGGCCAATC TCACATTGCC TCCCGGTGGT 
CGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932839.1
CDS	831..5159
Translation	MPPVQLESSQLVVSGGPTSVPQPPPPGTVVASTAASHGYRLSTLIEFLLQRTYQELNVLADLLPRKSDMERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISNFLDQQTILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKNEKQTTLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADDKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVDRYLAGKFLTLIVWNQQVLGRKTGTASVHKVTVKIDETDCSKTLQISHEPPLPACDSKLMERAMRIDHLSIEKLLIDSVHARSHQKLQELKTILKSFNPSDNSFVETALPTLVIPILEPCGRSECLHVFVDLHSGMFQLMLYGFDQTTLDDMEKSINDDMKRIIPWLQQLKFWLGQQRCRHSVKHLPTVCSETLHLTNYTTHPISNLSRHSLYIKLTRLPQYYIVVEMFEVPGSPTQLEYAYHFLSVSHTEGEDNPPTALVLQKFKPNVEDLALDGACRKQVKAGNKRKLSGEQGSPESKKPKRTGEICAFNKVLAHFVAMCDSNMPFIGLRMELSTMEIPHQGVQIEGDGFSHAIRLLRVPPCKGTSEETQKALEQSLLDCTFRLQGRNNRTWVAELVFANCPLNSIVSREQGPTRHVNLTYENPLSEPVGGRKVVEMFLNDWNSIARLYDCVLEFSRSLQDIPAHLNMFCEVRVYNYRKLILCYGPTKGSSISIQWNSVHRKFHISLGTVGPNSGCSNCHNIILHQLQEMFNKVPNVVQLLQVLYDTQAPLTSINKLPTVPMLGLTQRINTAYQCFSILPQSPTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLLSQLQPQGQQTQQFTKQMGASGAYPLTSPPSSYHNTVTPSPSMMHSQSPGNLQSSPSGVLRAPSPASFGPTPSPSSLGISMVPTSNFASPHGALDPSSPYTMMSPSQRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQSMPTNMPPPRKLPQRSWAASIPTILTHSALHVLLLPSPTPGLAPALAGSYLCSPLERFLGSVNMRRHLQRIIQQETLQLINSNEPGVIMFKTDVLKCRVALNPTTNQTLQLKVTPENAGQWKPEELQVLERFFETRVAGPPFKANTLIAFTKLLGAPAHILRDCVHIMKLELFPDQTTQLKWNVQFCLTIPPSAPPIAPSGTPAVVLKSKMLFFLQLTQKTSAPTDAFSIIVPIIYDMASGTTQQAEIPRQQNSSVTAPLTVSNILKRFAEMNPPRPGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_002932793.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932793.5

ORF Insert Sequence:

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTGCACTA 
GACCCTAGTT CTCCGTACAC CATGATGTCA CCCAGCCAGC GTGCAGGAAA TTGGCCAGGG 
TCCCCTCAAG TATCTGGTCC TTCTCCAGCA GCTCGTATGC CTGGAATGTC ACCAGCAAAT 
CCATCTCTTC ATTCTCCTGT CCCTGACGCG TCTCACTCCC CTCGTGCTGG AACAAGTTCT 
CAGTCAATGC CCACAAATAT GCCTCCTCCG CGTAAGCTAC CTCAGCGATC TTGGGCCGCA 
TCGATTCCTA CAATACTCAC TCACAGTGCC TTGCATGTGC TTTTACTGCC TTCTCCTACA 
CCTGGATTAG CCCCAGCATT GGCAGGCAGC TACCTGTGTT CCCCTCTTGA AAGGTTCTTG 
GGATCTGTCA ATATGAGACG ACACCTGCAA AGGATCATTC AGCAGGAAAC GCTGCAGTTA 
ATAAATTCCA ATGAACCAGG GGTGATAATG TTCAAAACAG ATGTATTAAA GTGCCGTGTG 
GCTCTTAACC CAACTACCAA CCAAACTTTG CAACTAAAAG TTACACCTGA GAATGCTGGA 
CAGTGGAAGC CAGAAGAGCT TCAAGTTTTA GAGAGGTTCT TTGAGACCAG GGTTGCAGGA 
CCTCCATTCA AGGCAAATAC TTTGATAGCT TTCACCAAGT TATTAGGAGC ACCTGCGCAT 
ATCCTCAGAG ACTGTGTGCA TATAATGAAG CTAGAACTGT TTCCAGATCA GACAACCCAA 
CTGAAATGGA ATGTGCAGTT CTGCCTAACC ATCCCACCTA GTGCACCACC AATAGCTCCA 
TCGGGAACTC CTGCTGTAGT ACTGAAATCT AAAATGTTGT TTTTTCTTCA GCTAACTCAA 
AAGACTTCAG CTCCAACAGA TGCTTTCAGT ATTATTGTTC CTATTATTTA TGATATGGCA 
TCAGGAACAA CCCAGCAAGC AGAAATCCCT CGGCAGCAGA ACTCTTCAGT AACGGCTCCC 
TTAACAGTTA GCAATATTCT GAAAAGGTTT GCTGAGATGA ATCCACCACG GCCAGGTGAA 
TGTACAATAT TTGCTGCAGT ACGAGATTTA ATGGCCAATC TCACATTGCC TCCCGGTGGT 
CGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med14 ( XM_004911772.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911829.1 Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa05805
Clone ID Related Accession (Same CDS sequence)	XM_004911772.3 , XM_004911772.4
Accession Version	XM_004911772.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911772.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTGCACTA 
GACCCTAGTT CTCCGTACAC CATGATGTCA CCCAGCCAGC GTGCAGGAAA TTGGCCAGGG 
TCCCCTCAAG TATCTGGTCC TTCTCCAGCA GCTCGTATGC CTGGAATGTC ACCAGCAAAT 
CCATCTCTTC ATTCTCCTGT CCCTGACGCG TCTCACTCCC CTCGTGCTGG AACAAGTTCT 
CAGTCAATGC CCACAAATAT GCCTCCTCCG CGTAAGCTAC CTCAGCGATC TTGGGCCGCA 
TCGATTCCTA CAATACTCAC TCACAGTGCC TTGCATGTGC TTTTACTGCC TTCTCCTACA 
CCTGGATTAG CCCCAGCATT GGCAGGCAGC TACCTGTGTT CCCCTCTTGA AAGGTTCTTG 
GGATCTGTCA ATATGAGACG ACACCTGCAA AGGATCATTC AGCAGGAAAC GTTAATAAAT 
TCCAATGAAC CAGGGGTGAT AATGTTCAAA ACAGATGTAT TAAAGTGCCG TGTGGCTCTT 
AACCCAACTA CCAACCAAAC TTTGCAACTA AAAGTTACAC CTGAGAATGC TGGACAGTGG 
AAGCCAGAAG AGCTTCAAGT TTTAGAGAGG TTCTTTGAGA CCAGGGTTGC AGGACCTCCA 
TTCAAGGCAA ATACTTTGAT AGCTTTCACC AAGTTATTAG GAGCACCTGC GCATATCCTC 
AGAGACTGTG TGCATATAAT GAAGCTAGAA CTGTTTCCAG ATCAGACAAC CCAACTGAAA 
TGGAATGTGC AGTTCTGCCT AACCATCCCA CCTAGTGCAC CACCAATAGC TCCATCGGGA 
ACTCCTGCTG TAGTACTGAA ATCTAAAATG TTGTTTTTTC TTCAGCTAAC TCAAAAGACT 
TCAGCTCCAA CAGATGCTTT CAGTATTATT GTTCCTATTA TTTATGATAT GGCATCAGGA 
ACAACCCAGC AAGCAGAAAT CCCTCGGCAG CAGAACTCTT CAGTAACGGC TCCCTTAACA 
GTTAGCAATA TTCTGAAAAG GTTTGCTGAG ATGAATCCAC CACGGCCAGG TGAATGTACA 
ATATTTGCTG CAGTACGAGA TTTAATGGCC AATCTCACAT TGCCTCCCGG TGGTCGACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911829.1
CDS	832..5154
Translation	MPPVQLESSQLVVSGGPTSVPQPPPPGTVVASTAASHGYRLSTLIEFLLQRTYQELNVLADLLPRKSDMERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISNFLDQQTILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKNEKQTTLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADDKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVDRYLAGKFLTLIVWNQQVLGRKTGTASVHKVTVKIDETDCSKTLQISHEPPLPACDSKLMERAMRIDHLSIEKLLIDSVHARSHQKLQELKTILKSFNPSDNSFVETALPTLVIPILEPCGRSECLHVFVDLHSGMFQLMLYGFDQTTLDDMEKSINDDMKRIIPWLQQLKFWLGQQRCRHSVKHLPTVCSETLHLTNYTTHPISNLSRHSLYIKLTRLPQYYIVVEMFEVPGSPTQLEYAYHFLSVSHTEGEDNPPTALVLQKFKPNVEDLALDGACRKQVKAGNKRKLSGEQGSPESKKPKRTGEICAFNKVLAHFVAMCDSNMPFIGLRMELSTMEIPHQGVQIEGDGFSHAIRLLRVPPCKGTSEETQKALEQSLLDCTFRLQGRNNRTWVAELVFANCPLNSIVSREQGPTRHVNLTYENPLSEPVGGRKVVEMFLNDWNSIARLYDCVLEFSRSLQDIPAHLNMFCEVRVYNYRKLILCYGPTKGSSISIQWNSVHRKFHISLGTVGPNSGCSNCHNIILHQLQEMFNKVPNVVQLLQVLYDTQAPLTSINKLPTVPMLGLTQRINTAYQCFSILPQSPTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLLSQLQPQGQQTQQFTKQMGASGAYPLTSPPSSYHNTVTPSPSMMHSQSPGNLQSSPSGVLRAPSPASFGPTPSPSSLGISMVPTSNFASPHGALDPSSPYTMMSPSQRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQSMPTNMPPPRKLPQRSWAASIPTILTHSALHVLLLPSPTPGLAPALAGSYLCSPLERFLGSVNMRRHLQRIIQQETLINSNEPGVIMFKTDVLKCRVALNPTTNQTLQLKVTPENAGQWKPEELQVLERFFETRVAGPPFKANTLIAFTKLLGAPAHILRDCVHIMKLELFPDQTTQLKWNVQFCLTIPPSAPPIAPSGTPAVVLKSKMLFFLQLTQKTSAPTDAFSIIVPIIYDMASGTTQQAEIPRQQNSSVTAPLTVSNILKRFAEMNPPRPGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_004911772.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911772.4

ORF Insert Sequence:

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTGCACTA 
GACCCTAGTT CTCCGTACAC CATGATGTCA CCCAGCCAGC GTGCAGGAAA TTGGCCAGGG 
TCCCCTCAAG TATCTGGTCC TTCTCCAGCA GCTCGTATGC CTGGAATGTC ACCAGCAAAT 
CCATCTCTTC ATTCTCCTGT CCCTGACGCG TCTCACTCCC CTCGTGCTGG AACAAGTTCT 
CAGTCAATGC CCACAAATAT GCCTCCTCCG CGTAAGCTAC CTCAGCGATC TTGGGCCGCA 
TCGATTCCTA CAATACTCAC TCACAGTGCC TTGCATGTGC TTTTACTGCC TTCTCCTACA 
CCTGGATTAG CCCCAGCATT GGCAGGCAGC TACCTGTGTT CCCCTCTTGA AAGGTTCTTG 
GGATCTGTCA ATATGAGACG ACACCTGCAA AGGATCATTC AGCAGGAAAC GTTAATAAAT 
TCCAATGAAC CAGGGGTGAT AATGTTCAAA ACAGATGTAT TAAAGTGCCG TGTGGCTCTT 
AACCCAACTA CCAACCAAAC TTTGCAACTA AAAGTTACAC CTGAGAATGC TGGACAGTGG 
AAGCCAGAAG AGCTTCAAGT TTTAGAGAGG TTCTTTGAGA CCAGGGTTGC AGGACCTCCA 
TTCAAGGCAA ATACTTTGAT AGCTTTCACC AAGTTATTAG GAGCACCTGC GCATATCCTC 
AGAGACTGTG TGCATATAAT GAAGCTAGAA CTGTTTCCAG ATCAGACAAC CCAACTGAAA 
TGGAATGTGC AGTTCTGCCT AACCATCCCA CCTAGTGCAC CACCAATAGC TCCATCGGGA 
ACTCCTGCTG TAGTACTGAA ATCTAAAATG TTGTTTTTTC TTCAGCTAAC TCAAAAGACT 
TCAGCTCCAA CAGATGCTTT CAGTATTATT GTTCCTATTA TTTATGATAT GGCATCAGGA 
ACAACCCAGC AAGCAGAAAT CCCTCGGCAG CAGAACTCTT CAGTAACGGC TCCCTTAACA 
GTTAGCAATA TTCTGAAAAG GTTTGCTGAG ATGAATCCAC CACGGCCAGG TGAATGTACA 
ATATTTGCTG CAGTACGAGA TTTAATGGCC AATCTCACAT TGCCTCCCGG TGGTCGACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med14 ( XM_004911773.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911830.1 Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X3, mRNA.

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CloneID	OXa05806
Clone ID Related Accession (Same CDS sequence)	XM_004911773.3 , XM_004911773.4
Accession Version	XM_004911773.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911773.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTTCTCAG 
TCAATGCCCA CAAATATGCC TCCTCCGCGT AAGCTACCTC AGCGATCTTG GGCCGCATCG 
ATTCCTACAA TACTCACTCA CAGTGCCTTG CATGTGCTTT TACTGCCTTC TCCTACACCT 
GGATTAGCCC CAGCATTGGC AGGCAGCTAC CTGTGTTCCC CTCTTGAAAG GTTCTTGGGA 
TCTGTCAATA TGAGACGACA CCTGCAAAGG ATCATTCAGC AGGAAACGCT GCAGTTAATA 
AATTCCAATG AACCAGGGGT GATAATGTTC AAAACAGATG TATTAAAGTG CCGTGTGGCT 
CTTAACCCAA CTACCAACCA AACTTTGCAA CTAAAAGTTA CACCTGAGAA TGCTGGACAG 
TGGAAGCCAG AAGAGCTTCA AGTTTTAGAG AGGTTCTTTG AGACCAGGGT TGCAGGACCT 
CCATTCAAGG CAAATACTTT GATAGCTTTC ACCAAGTTAT TAGGAGCACC TGCGCATATC 
CTCAGAGACT GTGTGCATAT AATGAAGCTA GAACTGTTTC CAGATCAGAC AACCCAACTG 
AAATGGAATG TGCAGTTCTG CCTAACCATC CCACCTAGTG CACCACCAAT AGCTCCATCG 
GGAACTCCTG CTGTAGTACT GAAATCTAAA ATGTTGTTTT TTCTTCAGCT AACTCAAAAG 
ACTTCAGCTC CAACAGATGC TTTCAGTATT ATTGTTCCTA TTATTTATGA TATGGCATCA 
GGAACAACCC AGCAAGCAGA AATCCCTCGG CAGCAGAACT CTTCAGTAAC GGCTCCCTTA 
ACAGTTAGCA ATATTCTGAA AAGGTTTGCT GAGATGAATC CACCACGGCC AGGTGAATGT 
ACAATATTTG CTGCAGTACG AGATTTAATG GCCAATCTCA CATTGCCTCC CGGTGGTCGA 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911830.1
CDS	831..4976
Translation	MPPVQLESSQLVVSGGPTSVPQPPPPGTVVASTAASHGYRLSTLIEFLLQRTYQELNVLADLLPRKSDMERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISNFLDQQTILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKNEKQTTLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADDKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVDRYLAGKFLTLIVWNQQVLGRKTGTASVHKVTVKIDETDCSKTLQISHEPPLPACDSKLMERAMRIDHLSIEKLLIDSVHARSHQKLQELKTILKSFNPSDNSFVETALPTLVIPILEPCGRSECLHVFVDLHSGMFQLMLYGFDQTTLDDMEKSINDDMKRIIPWLQQLKFWLGQQRCRHSVKHLPTVCSETLHLTNYTTHPISNLSRHSLYIKLTRLPQYYIVVEMFEVPGSPTQLEYAYHFLSVSHTEGEDNPPTALVLQKFKPNVEDLALDGACRKQVKAGNKRKLSGEQGSPESKKPKRTGEICAFNKVLAHFVAMCDSNMPFIGLRMELSTMEIPHQGVQIEGDGFSHAIRLLRVPPCKGTSEETQKALEQSLLDCTFRLQGRNNRTWVAELVFANCPLNSIVSREQGPTRHVNLTYENPLSEPVGGRKVVEMFLNDWNSIARLYDCVLEFSRSLQDIPAHLNMFCEVRVYNYRKLILCYGPTKGSSISIQWNSVHRKFHISLGTVGPNSGCSNCHNIILHQLQEMFNKVPNVVQLLQVLYDTQAPLTSINKLPTVPMLGLTQRINTAYQCFSILPQSPTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLLSQLQPQGQQTQQFTKQMGASGAYPLTSPPSSYHNTVTPSPSMMHSQSPGNLQSSPSGVLRAPSPASFGPTPSPSSLGISMVPTSNFASPHGSQSMPTNMPPPRKLPQRSWAASIPTILTHSALHVLLLPSPTPGLAPALAGSYLCSPLERFLGSVNMRRHLQRIIQQETLQLINSNEPGVIMFKTDVLKCRVALNPTTNQTLQLKVTPENAGQWKPEELQVLERFFETRVAGPPFKANTLIAFTKLLGAPAHILRDCVHIMKLELFPDQTTQLKWNVQFCLTIPPSAPPIAPSGTPAVVLKSKMLFFLQLTQKTSAPTDAFSIIVPIIYDMASGTTQQAEIPRQQNSSVTAPLTVSNILKRFAEMNPPRPGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_004911773.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911773.4

ORF Insert Sequence:

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTTCTCAG 
TCAATGCCCA CAAATATGCC TCCTCCGCGT AAGCTACCTC AGCGATCTTG GGCCGCATCG 
ATTCCTACAA TACTCACTCA CAGTGCCTTG CATGTGCTTT TACTGCCTTC TCCTACACCT 
GGATTAGCCC CAGCATTGGC AGGCAGCTAC CTGTGTTCCC CTCTTGAAAG GTTCTTGGGA 
TCTGTCAATA TGAGACGACA CCTGCAAAGG ATCATTCAGC AGGAAACGCT GCAGTTAATA 
AATTCCAATG AACCAGGGGT GATAATGTTC AAAACAGATG TATTAAAGTG CCGTGTGGCT 
CTTAACCCAA CTACCAACCA AACTTTGCAA CTAAAAGTTA CACCTGAGAA TGCTGGACAG 
TGGAAGCCAG AAGAGCTTCA AGTTTTAGAG AGGTTCTTTG AGACCAGGGT TGCAGGACCT 
CCATTCAAGG CAAATACTTT GATAGCTTTC ACCAAGTTAT TAGGAGCACC TGCGCATATC 
CTCAGAGACT GTGTGCATAT AATGAAGCTA GAACTGTTTC CAGATCAGAC AACCCAACTG 
AAATGGAATG TGCAGTTCTG CCTAACCATC CCACCTAGTG CACCACCAAT AGCTCCATCG 
GGAACTCCTG CTGTAGTACT GAAATCTAAA ATGTTGTTTT TTCTTCAGCT AACTCAAAAG 
ACTTCAGCTC CAACAGATGC TTTCAGTATT ATTGTTCCTA TTATTTATGA TATGGCATCA 
GGAACAACCC AGCAAGCAGA AATCCCTCGG CAGCAGAACT CTTCAGTAAC GGCTCCCTTA 
ACAGTTAGCA ATATTCTGAA AAGGTTTGCT GAGATGAATC CACCACGGCC AGGTGAATGT 
ACAATATTTG CTGCAGTACG AGATTTAATG GCCAATCTCA CATTGCCTCC CGGTGGTCGA 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med14 ( XM_004911774.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911831.1 Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X4, mRNA.

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CloneID	OXa05807
Clone ID Related Accession (Same CDS sequence)	XM_004911774.4 , XM_004911774.3
Accession Version	XM_004911774.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911774.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTTCTCAG 
TCAATGCCCA CAAATATGCC TCCTCCGCGT AAGCTACCTC AGCGATCTTG GGCCGCATCG 
ATTCCTACAA TACTCACTCA CAGTGCCTTG CATGTGCTTT TACTGCCTTC TCCTACACCT 
GGATTAGCCC CAGCATTGGC AGGCAGCTAC CTGTGTTCCC CTCTTGAAAG GTTCTTGGGA 
TCTGTCAATA TGAGACGACA CCTGCAAAGG ATCATTCAGC AGGAAACGTT AATAAATTCC 
AATGAACCAG GGGTGATAAT GTTCAAAACA GATGTATTAA AGTGCCGTGT GGCTCTTAAC 
CCAACTACCA ACCAAACTTT GCAACTAAAA GTTACACCTG AGAATGCTGG ACAGTGGAAG 
CCAGAAGAGC TTCAAGTTTT AGAGAGGTTC TTTGAGACCA GGGTTGCAGG ACCTCCATTC 
AAGGCAAATA CTTTGATAGC TTTCACCAAG TTATTAGGAG CACCTGCGCA TATCCTCAGA 
GACTGTGTGC ATATAATGAA GCTAGAACTG TTTCCAGATC AGACAACCCA ACTGAAATGG 
AATGTGCAGT TCTGCCTAAC CATCCCACCT AGTGCACCAC CAATAGCTCC ATCGGGAACT 
CCTGCTGTAG TACTGAAATC TAAAATGTTG TTTTTTCTTC AGCTAACTCA AAAGACTTCA 
GCTCCAACAG ATGCTTTCAG TATTATTGTT CCTATTATTT ATGATATGGC ATCAGGAACA 
ACCCAGCAAG CAGAAATCCC TCGGCAGCAG AACTCTTCAG TAACGGCTCC CTTAACAGTT 
AGCAATATTC TGAAAAGGTT TGCTGAGATG AATCCACCAC GGCCAGGTGA ATGTACAATA 
TTTGCTGCAG TACGAGATTT AATGGCCAAT CTCACATTGC CTCCCGGTGG TCGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911831.1
CDS	831..4970
Translation	MPPVQLESSQLVVSGGPTSVPQPPPPGTVVASTAASHGYRLSTLIEFLLQRTYQELNVLADLLPRKSDMERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISNFLDQQTILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKNEKQTTLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADDKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVDRYLAGKFLTLIVWNQQVLGRKTGTASVHKVTVKIDETDCSKTLQISHEPPLPACDSKLMERAMRIDHLSIEKLLIDSVHARSHQKLQELKTILKSFNPSDNSFVETALPTLVIPILEPCGRSECLHVFVDLHSGMFQLMLYGFDQTTLDDMEKSINDDMKRIIPWLQQLKFWLGQQRCRHSVKHLPTVCSETLHLTNYTTHPISNLSRHSLYIKLTRLPQYYIVVEMFEVPGSPTQLEYAYHFLSVSHTEGEDNPPTALVLQKFKPNVEDLALDGACRKQVKAGNKRKLSGEQGSPESKKPKRTGEICAFNKVLAHFVAMCDSNMPFIGLRMELSTMEIPHQGVQIEGDGFSHAIRLLRVPPCKGTSEETQKALEQSLLDCTFRLQGRNNRTWVAELVFANCPLNSIVSREQGPTRHVNLTYENPLSEPVGGRKVVEMFLNDWNSIARLYDCVLEFSRSLQDIPAHLNMFCEVRVYNYRKLILCYGPTKGSSISIQWNSVHRKFHISLGTVGPNSGCSNCHNIILHQLQEMFNKVPNVVQLLQVLYDTQAPLTSINKLPTVPMLGLTQRINTAYQCFSILPQSPTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLLSQLQPQGQQTQQFTKQMGASGAYPLTSPPSSYHNTVTPSPSMMHSQSPGNLQSSPSGVLRAPSPASFGPTPSPSSLGISMVPTSNFASPHGSQSMPTNMPPPRKLPQRSWAASIPTILTHSALHVLLLPSPTPGLAPALAGSYLCSPLERFLGSVNMRRHLQRIIQQETLINSNEPGVIMFKTDVLKCRVALNPTTNQTLQLKVTPENAGQWKPEELQVLERFFETRVAGPPFKANTLIAFTKLLGAPAHILRDCVHIMKLELFPDQTTQLKWNVQFCLTIPPSAPPIAPSGTPAVVLKSKMLFFLQLTQKTSAPTDAFSIIVPIIYDMASGTTQQAEIPRQQNSSVTAPLTVSNILKRFAEMNPPRPGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_004911774.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911774.4

ORF Insert Sequence:

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTTCTCAG 
TCAATGCCCA CAAATATGCC TCCTCCGCGT AAGCTACCTC AGCGATCTTG GGCCGCATCG 
ATTCCTACAA TACTCACTCA CAGTGCCTTG CATGTGCTTT TACTGCCTTC TCCTACACCT 
GGATTAGCCC CAGCATTGGC AGGCAGCTAC CTGTGTTCCC CTCTTGAAAG GTTCTTGGGA 
TCTGTCAATA TGAGACGACA CCTGCAAAGG ATCATTCAGC AGGAAACGTT AATAAATTCC 
AATGAACCAG GGGTGATAAT GTTCAAAACA GATGTATTAA AGTGCCGTGT GGCTCTTAAC 
CCAACTACCA ACCAAACTTT GCAACTAAAA GTTACACCTG AGAATGCTGG ACAGTGGAAG 
CCAGAAGAGC TTCAAGTTTT AGAGAGGTTC TTTGAGACCA GGGTTGCAGG ACCTCCATTC 
AAGGCAAATA CTTTGATAGC TTTCACCAAG TTATTAGGAG CACCTGCGCA TATCCTCAGA 
GACTGTGTGC ATATAATGAA GCTAGAACTG TTTCCAGATC AGACAACCCA ACTGAAATGG 
AATGTGCAGT TCTGCCTAAC CATCCCACCT AGTGCACCAC CAATAGCTCC ATCGGGAACT 
CCTGCTGTAG TACTGAAATC TAAAATGTTG TTTTTTCTTC AGCTAACTCA AAAGACTTCA 
GCTCCAACAG ATGCTTTCAG TATTATTGTT CCTATTATTT ATGATATGGC ATCAGGAACA 
ACCCAGCAAG CAGAAATCCC TCGGCAGCAG AACTCTTCAG TAACGGCTCC CTTAACAGTT 
AGCAATATTC TGAAAAGGTT TGCTGAGATG AATCCACCAC GGCCAGGTGA ATGTACAATA 
TTTGCTGCAG TACGAGATTT AATGGCCAAT CTCACATTGC CTCCCGGTGG TCGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med14 ( XM_002932793.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932839.1 Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa05804
Clone ID Related Accession (Same CDS sequence)	XM_002932793.4 , XM_002932793.5
Accession Version	XM_002932793.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932793.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTGCACTA 
GACCCTAGTT CTCCGTACAC CATGATGTCA CCCAGCCAGC GTGCAGGAAA TTGGCCAGGG 
TCCCCTCAAG TATCTGGTCC TTCTCCAGCA GCTCGTATGC CTGGAATGTC ACCAGCAAAT 
CCATCTCTTC ATTCTCCTGT CCCTGACGCG TCTCACTCCC CTCGTGCTGG AACAAGTTCT 
CAGTCAATGC CCACAAATAT GCCTCCTCCG CGTAAGCTAC CTCAGCGATC TTGGGCCGCA 
TCGATTCCTA CAATACTCAC TCACAGTGCC TTGCATGTGC TTTTACTGCC TTCTCCTACA 
CCTGGATTAG CCCCAGCATT GGCAGGCAGC TACCTGTGTT CCCCTCTTGA AAGGTTCTTG 
GGATCTGTCA ATATGAGACG ACACCTGCAA AGGATCATTC AGCAGGAAAC GCTGCAGTTA 
ATAAATTCCA ATGAACCAGG GGTGATAATG TTCAAAACAG ATGTATTAAA GTGCCGTGTG 
GCTCTTAACC CAACTACCAA CCAAACTTTG CAACTAAAAG TTACACCTGA GAATGCTGGA 
CAGTGGAAGC CAGAAGAGCT TCAAGTTTTA GAGAGGTTCT TTGAGACCAG GGTTGCAGGA 
CCTCCATTCA AGGCAAATAC TTTGATAGCT TTCACCAAGT TATTAGGAGC ACCTGCGCAT 
ATCCTCAGAG ACTGTGTGCA TATAATGAAG CTAGAACTGT TTCCAGATCA GACAACCCAA 
CTGAAATGGA ATGTGCAGTT CTGCCTAACC ATCCCACCTA GTGCACCACC AATAGCTCCA 
TCGGGAACTC CTGCTGTAGT ACTGAAATCT AAAATGTTGT TTTTTCTTCA GCTAACTCAA 
AAGACTTCAG CTCCAACAGA TGCTTTCAGT ATTATTGTTC CTATTATTTA TGATATGGCA 
TCAGGAACAA CCCAGCAAGC AGAAATCCCT CGGCAGCAGA ACTCTTCAGT AACGGCTCCC 
TTAACAGTTA GCAATATTCT GAAAAGGTTT GCTGAGATGA ATCCACCACG GCCAGGTGAA 
TGTACAATAT TTGCTGCAGT ACGAGATTTA ATGGCCAATC TCACATTGCC TCCCGGTGGT 
CGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932839.1
CDS	827..5155
Translation	MPPVQLESSQLVVSGGPTSVPQPPPPGTVVASTAASHGYRLSTLIEFLLQRTYQELNVLADLLPRKSDMERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISNFLDQQTILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKNEKQTTLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADDKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVDRYLAGKFLTLIVWNQQVLGRKTGTASVHKVTVKIDETDCSKTLQISHEPPLPACDSKLMERAMRIDHLSIEKLLIDSVHARSHQKLQELKTILKSFNPSDNSFVETALPTLVIPILEPCGRSECLHVFVDLHSGMFQLMLYGFDQTTLDDMEKSINDDMKRIIPWLQQLKFWLGQQRCRHSVKHLPTVCSETLHLTNYTTHPISNLSRHSLYIKLTRLPQYYIVVEMFEVPGSPTQLEYAYHFLSVSHTEGEDNPPTALVLQKFKPNVEDLALDGACRKQVKAGNKRKLSGEQGSPESKKPKRTGEICAFNKVLAHFVAMCDSNMPFIGLRMELSTMEIPHQGVQIEGDGFSHAIRLLRVPPCKGTSEETQKALEQSLLDCTFRLQGRNNRTWVAELVFANCPLNSIVSREQGPTRHVNLTYENPLSEPVGGRKVVEMFLNDWNSIARLYDCVLEFSRSLQDIPAHLNMFCEVRVYNYRKLILCYGPTKGSSISIQWNSVHRKFHISLGTVGPNSGCSNCHNIILHQLQEMFNKVPNVVQLLQVLYDTQAPLTSINKLPTVPMLGLTQRINTAYQCFSILPQSPTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLLSQLQPQGQQTQQFTKQMGASGAYPLTSPPSSYHNTVTPSPSMMHSQSPGNLQSSPSGVLRAPSPASFGPTPSPSSLGISMVPTSNFASPHGALDPSSPYTMMSPSQRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQSMPTNMPPPRKLPQRSWAASIPTILTHSALHVLLLPSPTPGLAPALAGSYLCSPLERFLGSVNMRRHLQRIIQQETLQLINSNEPGVIMFKTDVLKCRVALNPTTNQTLQLKVTPENAGQWKPEELQVLERFFETRVAGPPFKANTLIAFTKLLGAPAHILRDCVHIMKLELFPDQTTQLKWNVQFCLTIPPSAPPIAPSGTPAVVLKSKMLFFLQLTQKTSAPTDAFSIIVPIIYDMASGTTQQAEIPRQQNSSVTAPLTVSNILKRFAEMNPPRPGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_002932793.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932793.4

ORF Insert Sequence:

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTGCACTA 
GACCCTAGTT CTCCGTACAC CATGATGTCA CCCAGCCAGC GTGCAGGAAA TTGGCCAGGG 
TCCCCTCAAG TATCTGGTCC TTCTCCAGCA GCTCGTATGC CTGGAATGTC ACCAGCAAAT 
CCATCTCTTC ATTCTCCTGT CCCTGACGCG TCTCACTCCC CTCGTGCTGG AACAAGTTCT 
CAGTCAATGC CCACAAATAT GCCTCCTCCG CGTAAGCTAC CTCAGCGATC TTGGGCCGCA 
TCGATTCCTA CAATACTCAC TCACAGTGCC TTGCATGTGC TTTTACTGCC TTCTCCTACA 
CCTGGATTAG CCCCAGCATT GGCAGGCAGC TACCTGTGTT CCCCTCTTGA AAGGTTCTTG 
GGATCTGTCA ATATGAGACG ACACCTGCAA AGGATCATTC AGCAGGAAAC GCTGCAGTTA 
ATAAATTCCA ATGAACCAGG GGTGATAATG TTCAAAACAG ATGTATTAAA GTGCCGTGTG 
GCTCTTAACC CAACTACCAA CCAAACTTTG CAACTAAAAG TTACACCTGA GAATGCTGGA 
CAGTGGAAGC CAGAAGAGCT TCAAGTTTTA GAGAGGTTCT TTGAGACCAG GGTTGCAGGA 
CCTCCATTCA AGGCAAATAC TTTGATAGCT TTCACCAAGT TATTAGGAGC ACCTGCGCAT 
ATCCTCAGAG ACTGTGTGCA TATAATGAAG CTAGAACTGT TTCCAGATCA GACAACCCAA 
CTGAAATGGA ATGTGCAGTT CTGCCTAACC ATCCCACCTA GTGCACCACC AATAGCTCCA 
TCGGGAACTC CTGCTGTAGT ACTGAAATCT AAAATGTTGT TTTTTCTTCA GCTAACTCAA 
AAGACTTCAG CTCCAACAGA TGCTTTCAGT ATTATTGTTC CTATTATTTA TGATATGGCA 
TCAGGAACAA CCCAGCAAGC AGAAATCCCT CGGCAGCAGA ACTCTTCAGT AACGGCTCCC 
TTAACAGTTA GCAATATTCT GAAAAGGTTT GCTGAGATGA ATCCACCACG GCCAGGTGAA 
TGTACAATAT TTGCTGCAGT ACGAGATTTA ATGGCCAATC TCACATTGCC TCCCGGTGGT 
CGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med14 ( XM_004911772.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911829.1 Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa05805
Clone ID Related Accession (Same CDS sequence)	XM_004911772.3 , XM_004911772.4
Accession Version	XM_004911772.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911772.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTGCACTA 
GACCCTAGTT CTCCGTACAC CATGATGTCA CCCAGCCAGC GTGCAGGAAA TTGGCCAGGG 
TCCCCTCAAG TATCTGGTCC TTCTCCAGCA GCTCGTATGC CTGGAATGTC ACCAGCAAAT 
CCATCTCTTC ATTCTCCTGT CCCTGACGCG TCTCACTCCC CTCGTGCTGG AACAAGTTCT 
CAGTCAATGC CCACAAATAT GCCTCCTCCG CGTAAGCTAC CTCAGCGATC TTGGGCCGCA 
TCGATTCCTA CAATACTCAC TCACAGTGCC TTGCATGTGC TTTTACTGCC TTCTCCTACA 
CCTGGATTAG CCCCAGCATT GGCAGGCAGC TACCTGTGTT CCCCTCTTGA AAGGTTCTTG 
GGATCTGTCA ATATGAGACG ACACCTGCAA AGGATCATTC AGCAGGAAAC GTTAATAAAT 
TCCAATGAAC CAGGGGTGAT AATGTTCAAA ACAGATGTAT TAAAGTGCCG TGTGGCTCTT 
AACCCAACTA CCAACCAAAC TTTGCAACTA AAAGTTACAC CTGAGAATGC TGGACAGTGG 
AAGCCAGAAG AGCTTCAAGT TTTAGAGAGG TTCTTTGAGA CCAGGGTTGC AGGACCTCCA 
TTCAAGGCAA ATACTTTGAT AGCTTTCACC AAGTTATTAG GAGCACCTGC GCATATCCTC 
AGAGACTGTG TGCATATAAT GAAGCTAGAA CTGTTTCCAG ATCAGACAAC CCAACTGAAA 
TGGAATGTGC AGTTCTGCCT AACCATCCCA CCTAGTGCAC CACCAATAGC TCCATCGGGA 
ACTCCTGCTG TAGTACTGAA ATCTAAAATG TTGTTTTTTC TTCAGCTAAC TCAAAAGACT 
TCAGCTCCAA CAGATGCTTT CAGTATTATT GTTCCTATTA TTTATGATAT GGCATCAGGA 
ACAACCCAGC AAGCAGAAAT CCCTCGGCAG CAGAACTCTT CAGTAACGGC TCCCTTAACA 
GTTAGCAATA TTCTGAAAAG GTTTGCTGAG ATGAATCCAC CACGGCCAGG TGAATGTACA 
ATATTTGCTG CAGTACGAGA TTTAATGGCC AATCTCACAT TGCCTCCCGG TGGTCGACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911829.1
CDS	831..5153
Translation	MPPVQLESSQLVVSGGPTSVPQPPPPGTVVASTAASHGYRLSTLIEFLLQRTYQELNVLADLLPRKSDMERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISNFLDQQTILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKNEKQTTLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADDKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVDRYLAGKFLTLIVWNQQVLGRKTGTASVHKVTVKIDETDCSKTLQISHEPPLPACDSKLMERAMRIDHLSIEKLLIDSVHARSHQKLQELKTILKSFNPSDNSFVETALPTLVIPILEPCGRSECLHVFVDLHSGMFQLMLYGFDQTTLDDMEKSINDDMKRIIPWLQQLKFWLGQQRCRHSVKHLPTVCSETLHLTNYTTHPISNLSRHSLYIKLTRLPQYYIVVEMFEVPGSPTQLEYAYHFLSVSHTEGEDNPPTALVLQKFKPNVEDLALDGACRKQVKAGNKRKLSGEQGSPESKKPKRTGEICAFNKVLAHFVAMCDSNMPFIGLRMELSTMEIPHQGVQIEGDGFSHAIRLLRVPPCKGTSEETQKALEQSLLDCTFRLQGRNNRTWVAELVFANCPLNSIVSREQGPTRHVNLTYENPLSEPVGGRKVVEMFLNDWNSIARLYDCVLEFSRSLQDIPAHLNMFCEVRVYNYRKLILCYGPTKGSSISIQWNSVHRKFHISLGTVGPNSGCSNCHNIILHQLQEMFNKVPNVVQLLQVLYDTQAPLTSINKLPTVPMLGLTQRINTAYQCFSILPQSPTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLLSQLQPQGQQTQQFTKQMGASGAYPLTSPPSSYHNTVTPSPSMMHSQSPGNLQSSPSGVLRAPSPASFGPTPSPSSLGISMVPTSNFASPHGALDPSSPYTMMSPSQRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQSMPTNMPPPRKLPQRSWAASIPTILTHSALHVLLLPSPTPGLAPALAGSYLCSPLERFLGSVNMRRHLQRIIQQETLINSNEPGVIMFKTDVLKCRVALNPTTNQTLQLKVTPENAGQWKPEELQVLERFFETRVAGPPFKANTLIAFTKLLGAPAHILRDCVHIMKLELFPDQTTQLKWNVQFCLTIPPSAPPIAPSGTPAVVLKSKMLFFLQLTQKTSAPTDAFSIIVPIIYDMASGTTQQAEIPRQQNSSVTAPLTVSNILKRFAEMNPPRPGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_004911772.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911772.3

ORF Insert Sequence:

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTGCACTA 
GACCCTAGTT CTCCGTACAC CATGATGTCA CCCAGCCAGC GTGCAGGAAA TTGGCCAGGG 
TCCCCTCAAG TATCTGGTCC TTCTCCAGCA GCTCGTATGC CTGGAATGTC ACCAGCAAAT 
CCATCTCTTC ATTCTCCTGT CCCTGACGCG TCTCACTCCC CTCGTGCTGG AACAAGTTCT 
CAGTCAATGC CCACAAATAT GCCTCCTCCG CGTAAGCTAC CTCAGCGATC TTGGGCCGCA 
TCGATTCCTA CAATACTCAC TCACAGTGCC TTGCATGTGC TTTTACTGCC TTCTCCTACA 
CCTGGATTAG CCCCAGCATT GGCAGGCAGC TACCTGTGTT CCCCTCTTGA AAGGTTCTTG 
GGATCTGTCA ATATGAGACG ACACCTGCAA AGGATCATTC AGCAGGAAAC GTTAATAAAT 
TCCAATGAAC CAGGGGTGAT AATGTTCAAA ACAGATGTAT TAAAGTGCCG TGTGGCTCTT 
AACCCAACTA CCAACCAAAC TTTGCAACTA AAAGTTACAC CTGAGAATGC TGGACAGTGG 
AAGCCAGAAG AGCTTCAAGT TTTAGAGAGG TTCTTTGAGA CCAGGGTTGC AGGACCTCCA 
TTCAAGGCAA ATACTTTGAT AGCTTTCACC AAGTTATTAG GAGCACCTGC GCATATCCTC 
AGAGACTGTG TGCATATAAT GAAGCTAGAA CTGTTTCCAG ATCAGACAAC CCAACTGAAA 
TGGAATGTGC AGTTCTGCCT AACCATCCCA CCTAGTGCAC CACCAATAGC TCCATCGGGA 
ACTCCTGCTG TAGTACTGAA ATCTAAAATG TTGTTTTTTC TTCAGCTAAC TCAAAAGACT 
TCAGCTCCAA CAGATGCTTT CAGTATTATT GTTCCTATTA TTTATGATAT GGCATCAGGA 
ACAACCCAGC AAGCAGAAAT CCCTCGGCAG CAGAACTCTT CAGTAACGGC TCCCTTAACA 
GTTAGCAATA TTCTGAAAAG GTTTGCTGAG ATGAATCCAC CACGGCCAGG TGAATGTACA 
ATATTTGCTG CAGTACGAGA TTTAATGGCC AATCTCACAT TGCCTCCCGG TGGTCGACCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med14 ( XM_004911773.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911830.1 Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X3, mRNA.

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CloneID	OXa05806
Clone ID Related Accession (Same CDS sequence)	XM_004911773.3 , XM_004911773.4
Accession Version	XM_004911773.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911773.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTTCTCAG 
TCAATGCCCA CAAATATGCC TCCTCCGCGT AAGCTACCTC AGCGATCTTG GGCCGCATCG 
ATTCCTACAA TACTCACTCA CAGTGCCTTG CATGTGCTTT TACTGCCTTC TCCTACACCT 
GGATTAGCCC CAGCATTGGC AGGCAGCTAC CTGTGTTCCC CTCTTGAAAG GTTCTTGGGA 
TCTGTCAATA TGAGACGACA CCTGCAAAGG ATCATTCAGC AGGAAACGCT GCAGTTAATA 
AATTCCAATG AACCAGGGGT GATAATGTTC AAAACAGATG TATTAAAGTG CCGTGTGGCT 
CTTAACCCAA CTACCAACCA AACTTTGCAA CTAAAAGTTA CACCTGAGAA TGCTGGACAG 
TGGAAGCCAG AAGAGCTTCA AGTTTTAGAG AGGTTCTTTG AGACCAGGGT TGCAGGACCT 
CCATTCAAGG CAAATACTTT GATAGCTTTC ACCAAGTTAT TAGGAGCACC TGCGCATATC 
CTCAGAGACT GTGTGCATAT AATGAAGCTA GAACTGTTTC CAGATCAGAC AACCCAACTG 
AAATGGAATG TGCAGTTCTG CCTAACCATC CCACCTAGTG CACCACCAAT AGCTCCATCG 
GGAACTCCTG CTGTAGTACT GAAATCTAAA ATGTTGTTTT TTCTTCAGCT AACTCAAAAG 
ACTTCAGCTC CAACAGATGC TTTCAGTATT ATTGTTCCTA TTATTTATGA TATGGCATCA 
GGAACAACCC AGCAAGCAGA AATCCCTCGG CAGCAGAACT CTTCAGTAAC GGCTCCCTTA 
ACAGTTAGCA ATATTCTGAA AAGGTTTGCT GAGATGAATC CACCACGGCC AGGTGAATGT 
ACAATATTTG CTGCAGTACG AGATTTAATG GCCAATCTCA CATTGCCTCC CGGTGGTCGA 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911830.1
CDS	829..4974
Translation	MPPVQLESSQLVVSGGPTSVPQPPPPGTVVASTAASHGYRLSTLIEFLLQRTYQELNVLADLLPRKSDMERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISNFLDQQTILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKNEKQTTLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADDKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVDRYLAGKFLTLIVWNQQVLGRKTGTASVHKVTVKIDETDCSKTLQISHEPPLPACDSKLMERAMRIDHLSIEKLLIDSVHARSHQKLQELKTILKSFNPSDNSFVETALPTLVIPILEPCGRSECLHVFVDLHSGMFQLMLYGFDQTTLDDMEKSINDDMKRIIPWLQQLKFWLGQQRCRHSVKHLPTVCSETLHLTNYTTHPISNLSRHSLYIKLTRLPQYYIVVEMFEVPGSPTQLEYAYHFLSVSHTEGEDNPPTALVLQKFKPNVEDLALDGACRKQVKAGNKRKLSGEQGSPESKKPKRTGEICAFNKVLAHFVAMCDSNMPFIGLRMELSTMEIPHQGVQIEGDGFSHAIRLLRVPPCKGTSEETQKALEQSLLDCTFRLQGRNNRTWVAELVFANCPLNSIVSREQGPTRHVNLTYENPLSEPVGGRKVVEMFLNDWNSIARLYDCVLEFSRSLQDIPAHLNMFCEVRVYNYRKLILCYGPTKGSSISIQWNSVHRKFHISLGTVGPNSGCSNCHNIILHQLQEMFNKVPNVVQLLQVLYDTQAPLTSINKLPTVPMLGLTQRINTAYQCFSILPQSPTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLLSQLQPQGQQTQQFTKQMGASGAYPLTSPPSSYHNTVTPSPSMMHSQSPGNLQSSPSGVLRAPSPASFGPTPSPSSLGISMVPTSNFASPHGSQSMPTNMPPPRKLPQRSWAASIPTILTHSALHVLLLPSPTPGLAPALAGSYLCSPLERFLGSVNMRRHLQRIIQQETLQLINSNEPGVIMFKTDVLKCRVALNPTTNQTLQLKVTPENAGQWKPEELQVLERFFETRVAGPPFKANTLIAFTKLLGAPAHILRDCVHIMKLELFPDQTTQLKWNVQFCLTIPPSAPPIAPSGTPAVVLKSKMLFFLQLTQKTSAPTDAFSIIVPIIYDMASGTTQQAEIPRQQNSSVTAPLTVSNILKRFAEMNPPRPGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_004911773.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911773.3

ORF Insert Sequence:

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTTCTCAG 
TCAATGCCCA CAAATATGCC TCCTCCGCGT AAGCTACCTC AGCGATCTTG GGCCGCATCG 
ATTCCTACAA TACTCACTCA CAGTGCCTTG CATGTGCTTT TACTGCCTTC TCCTACACCT 
GGATTAGCCC CAGCATTGGC AGGCAGCTAC CTGTGTTCCC CTCTTGAAAG GTTCTTGGGA 
TCTGTCAATA TGAGACGACA CCTGCAAAGG ATCATTCAGC AGGAAACGCT GCAGTTAATA 
AATTCCAATG AACCAGGGGT GATAATGTTC AAAACAGATG TATTAAAGTG CCGTGTGGCT 
CTTAACCCAA CTACCAACCA AACTTTGCAA CTAAAAGTTA CACCTGAGAA TGCTGGACAG 
TGGAAGCCAG AAGAGCTTCA AGTTTTAGAG AGGTTCTTTG AGACCAGGGT TGCAGGACCT 
CCATTCAAGG CAAATACTTT GATAGCTTTC ACCAAGTTAT TAGGAGCACC TGCGCATATC 
CTCAGAGACT GTGTGCATAT AATGAAGCTA GAACTGTTTC CAGATCAGAC AACCCAACTG 
AAATGGAATG TGCAGTTCTG CCTAACCATC CCACCTAGTG CACCACCAAT AGCTCCATCG 
GGAACTCCTG CTGTAGTACT GAAATCTAAA ATGTTGTTTT TTCTTCAGCT AACTCAAAAG 
ACTTCAGCTC CAACAGATGC TTTCAGTATT ATTGTTCCTA TTATTTATGA TATGGCATCA 
GGAACAACCC AGCAAGCAGA AATCCCTCGG CAGCAGAACT CTTCAGTAAC GGCTCCCTTA 
ACAGTTAGCA ATATTCTGAA AAGGTTTGCT GAGATGAATC CACCACGGCC AGGTGAATGT 
ACAATATTTG CTGCAGTACG AGATTTAATG GCCAATCTCA CATTGCCTCC CGGTGGTCGA 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

med14 ( XM_004911774.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911831.1 Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X4, mRNA.

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CloneID	OXa05807
Clone ID Related Accession (Same CDS sequence)	XM_004911774.4 , XM_004911774.3
Accession Version	XM_004911774.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTTCTCAG 
TCAATGCCCA CAAATATGCC TCCTCCGCGT AAGCTACCTC AGCGATCTTG GGCCGCATCG 
ATTCCTACAA TACTCACTCA CAGTGCCTTG CATGTGCTTT TACTGCCTTC TCCTACACCT 
GGATTAGCCC CAGCATTGGC AGGCAGCTAC CTGTGTTCCC CTCTTGAAAG GTTCTTGGGA 
TCTGTCAATA TGAGACGACA CCTGCAAAGG ATCATTCAGC AGGAAACGTT AATAAATTCC 
AATGAACCAG GGGTGATAAT GTTCAAAACA GATGTATTAA AGTGCCGTGT GGCTCTTAAC 
CCAACTACCA ACCAAACTTT GCAACTAAAA GTTACACCTG AGAATGCTGG ACAGTGGAAG 
CCAGAAGAGC TTCAAGTTTT AGAGAGGTTC TTTGAGACCA GGGTTGCAGG ACCTCCATTC 
AAGGCAAATA CTTTGATAGC TTTCACCAAG TTATTAGGAG CACCTGCGCA TATCCTCAGA 
GACTGTGTGC ATATAATGAA GCTAGAACTG TTTCCAGATC AGACAACCCA ACTGAAATGG 
AATGTGCAGT TCTGCCTAAC CATCCCACCT AGTGCACCAC CAATAGCTCC ATCGGGAACT 
CCTGCTGTAG TACTGAAATC TAAAATGTTG TTTTTTCTTC AGCTAACTCA AAAGACTTCA 
GCTCCAACAG ATGCTTTCAG TATTATTGTT CCTATTATTT ATGATATGGC ATCAGGAACA 
ACCCAGCAAG CAGAAATCCC TCGGCAGCAG AACTCTTCAG TAACGGCTCC CTTAACAGTT 
AGCAATATTC TGAAAAGGTT TGCTGAGATG AATCCACCAC GGCCAGGTGA ATGTACAATA 
TTTGCTGCAG TACGAGATTT AATGGCCAAT CTCACATTGC CTCCCGGTGG TCGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911831.1
CDS	832..4971
Translation	MPPVQLESSQLVVSGGPTSVPQPPPPGTVVASTAASHGYRLSTLIEFLLQRTYQELNVLADLLPRKSDMERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISNFLDQQTILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKNEKQTTLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDIPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADDKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVDRYLAGKFLTLIVWNQQVLGRKTGTASVHKVTVKIDETDCSKTLQISHEPPLPACDSKLMERAMRIDHLSIEKLLIDSVHARSHQKLQELKTILKSFNPSDNSFVETALPTLVIPILEPCGRSECLHVFVDLHSGMFQLMLYGFDQTTLDDMEKSINDDMKRIIPWLQQLKFWLGQQRCRHSVKHLPTVCSETLHLTNYTTHPISNLSRHSLYIKLTRLPQYYIVVEMFEVPGSPTQLEYAYHFLSVSHTEGEDNPPTALVLQKFKPNVEDLALDGACRKQVKAGNKRKLSGEQGSPESKKPKRTGEICAFNKVLAHFVAMCDSNMPFIGLRMELSTMEIPHQGVQIEGDGFSHAIRLLRVPPCKGTSEETQKALEQSLLDCTFRLQGRNNRTWVAELVFANCPLNSIVSREQGPTRHVNLTYENPLSEPVGGRKVVEMFLNDWNSIARLYDCVLEFSRSLQDIPAHLNMFCEVRVYNYRKLILCYGPTKGSSISIQWNSVHRKFHISLGTVGPNSGCSNCHNIILHQLQEMFNKVPNVVQLLQVLYDTQAPLTSINKLPTVPMLGLTQRINTAYQCFSILPQSPTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKIVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLLSQLQPQGQQTQQFTKQMGASGAYPLTSPPSSYHNTVTPSPSMMHSQSPGNLQSSPSGVLRAPSPASFGPTPSPSSLGISMVPTSNFASPHGSQSMPTNMPPPRKLPQRSWAASIPTILTHSALHVLLLPSPTPGLAPALAGSYLCSPLERFLGSVNMRRHLQRIIQQETLINSNEPGVIMFKTDVLKCRVALNPTTNQTLQLKVTPENAGQWKPEELQVLERFFETRVAGPPFKANTLIAFTKLLGAPAHILRDCVHIMKLELFPDQTTQLKWNVQFCLTIPPSAPPIAPSGTPAVVLKSKMLFFLQLTQKTSAPTDAFSIIVPIIYDMASGTTQQAEIPRQQNSSVTAPLTVSNILKRFAEMNPPRPGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_004911774.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mediator complex subunit 14 (med14), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911774.3

ORF Insert Sequence:

ATGCCACCAG TACAGCTAGA GAGCAGTCAG TTGGTGGTAT CTGGGGGGCC CACTTCTGTG 
CCTCAGCCTC CTCCACCAGG CACTGTAGTA GCCTCAACGG CTGCTAGTCA TGGCTACCGA 
CTCAGCACCC TGATTGAATT TTTACTTCAG CGGACTTATC AAGAACTCAA CGTATTGGCT 
GACTTACTTC CAAGGAAGAG TGACATGGAA AGAAAGATTG AAATAGTGCA GTTTGCAAGC 
CGAACACGGC AACTCTTTGT CAGATTATTG GCATTAGTAA AATGGGCCAA CAATGCTGGG 
AAAGTAGAAA AATGTGCGAT GATATCCAAT TTCTTAGATC AACAAACCAT ATTGTTTGTG 
GACACAGCTG ACCGACTAGC ATCATTAGCA AGAGATGCAC TTGTCCATGC TCGCTTACCT 
AGTTTTGCTA TTCCTTATGC TATTGATGTG CTTACAACAG GATCTTACCC AAGGTTACCA 
ACTTGTATTC GGGATAAAAT AATTCCCCCA GATCCCATAA CAAAAAATGA AAAGCAGACT 
ACACTTCATC AACTCAACCA GATTCTCAGG CATCGACTGG TAACTACAGA TCTTCCTCCA 
CAACTAGCGA ATCTTACTGT TGCCAATGGC CGTGTAAAGT TTCGAGTTGA AGGTGAATTT 
GAAGCAACGT TAACTGTTAT GGGTGATGAC CCAGATATCC CTTGGAGATT ACTGAAATTG 
GAAATACTTG TTGAGGATAA AGAAACTGGA GATGGCCGTG CATTAGTTCA CAGCATGCAG 
ATCAACTTTA TACACCAGCT TGTTCAGTCA AGACTTTTCG CTGACGATAA ACCACTTCAG 
GACATGTACA ACTGTTTACA TTCCTTTTGC CTGTCACTAC AGTTAGAAGT CCTTCATTCA 
CAGACATTAA TGCTGATCAG AGAACGTTGG GGGGATCTTG TCCAAGTGGA TCGATATCTT 
GCTGGAAAAT TTCTCACACT TATTGTCTGG AACCAACAAG TTCTTGGAAG AAAAACTGGA 
ACTGCTTCCG TCCACAAAGT CACAGTCAAG ATTGATGAAA CTGATTGCTC AAAAACTCTG 
CAGATTTCAC ATGAACCTCC TTTGCCTGCT TGTGATTCTA AACTAATGGA AAGAGCTATG 
CGGATAGACC ATCTCTCAAT AGAAAAGCTG CTTATAGACA GTGTTCATGC ACGGTCACAC 
CAGAAACTCC AGGAGCTGAA GACCATCCTA AAAAGCTTCA ACCCCAGTGA CAACTCTTTT 
GTAGAAACTG CTCTTCCAAC CCTGGTTATT CCCATCTTAG AGCCATGTGG CCGCTCTGAG 
TGTCTGCATG TATTTGTGGA CCTTCATTCT GGCATGTTCC AACTCATGTT GTATGGATTT 
GACCAGACCA CTTTAGATGA TATGGAGAAA TCTATCAATG ATGATATGAA GCGGATAATT 
CCTTGGCTCC AGCAGCTTAA GTTTTGGCTT GGGCAACAGC GTTGTAGACA TTCAGTAAAA 
CATCTGCCAA CAGTTTGCAG TGAAACACTT CATTTAACAA ACTACACAAC TCATCCAATC 
AGCAATCTTT CCAGGCATTC ACTATATATA AAACTCACCC GTCTTCCACA GTATTACATC 
GTTGTTGAGA TGTTTGAGGT TCCTGGTAGC CCGACTCAGT TGGAATATGC CTACCACTTT 
CTGTCTGTTA GTCACACAGA AGGCGAGGAT AACCCTCCTA CTGCTCTTGT GCTTCAGAAG 
TTCAAACCAA ATGTTGAAGA TCTTGCATTA GATGGAGCAT GTCGAAAGCA AGTAAAGGCC 
GGCAATAAGC GCAAGCTTTC TGGAGAACAG GGCTCTCCGG AATCAAAGAA ACCAAAGCGT 
ACAGGAGAGA TCTGTGCCTT TAACAAAGTC CTTGCTCACT TTGTAGCCAT GTGTGACTCT 
AACATGCCAT TTATTGGCCT TCGGATGGAG CTATCCACAA TGGAGATCCC ACATCAGGGT 
GTTCAGATAG AAGGAGATGG TTTCAGTCAT GCAATCCGAT TATTAAGGGT GCCACCATGC 
AAAGGCACAA GTGAAGAAAC CCAGAAAGCA CTTGAGCAGT CTCTTCTTGA TTGCACGTTT 
CGTCTACAAG GAAGGAACAA TCGCACATGG GTCGCTGAGC TGGTGTTTGC TAATTGCCCT 
TTAAACAGCA TTGTATCTAG AGAGCAAGGA CCTACACGAC ATGTTAACCT AACATATGAA 
AACCCACTTT CTGAACCAGT AGGTGGACGC AAAGTTGTAG AAATGTTTCT GAATGACTGG 
AATAGTATTG CACGTCTTTA TGACTGTGTA TTGGAGTTTT CGCGGAGTTT ACAAGACATA 
CCGGCTCACT TAAATATGTT CTGTGAAGTT CGTGTTTACA ACTATCGCAA ACTTATTCTG 
TGTTATGGAC CCACAAAGGG AAGCTCGATC AGCATTCAGT GGAATTCTGT CCACCGTAAG 
TTTCACATTT CACTTGGCAC AGTTGGACCT AATTCAGGGT GCAGCAACTG TCACAATATC 
ATTCTTCATC AGCTGCAGGA AATGTTCAAC AAAGTCCCAA ATGTGGTGCA GTTATTGCAG 
GTATTATATG ACACCCAAGC TCCATTAACT TCTATCAACA AACTTCCAAC TGTGCCAATG 
CTGGGACTCA CCCAGCGAAT AAACACAGCC TATCAGTGCT TTTCTATCCT TCCTCAGTCT 
CCTACACACA TCCGACTGGC TTTCAGAAAT ATGTATTGCA TAGATATTTA TTGTAGAAGT 
CGAGGTGTTG TAGCCATACG TGATGGAGCT TACAGTCTCT TCGACAACAG CAAAATAGTG 
GAAGGCTTTT ATCCTGCTCC TGGGCTAAAG ACTTTTCTAA ATATGTTTGT TGATAGTAAT 
CAGGATGCAC GTCGACGATC AGTCAATGAA GACGACAATC CACCATCTCC TATTGGAGGT 
GATATGATGG ACTCCTTGTT ATCCCAATTG CAACCACAAG GGCAGCAAAC ACAGCAGTTT 
ACAAAGCAAA TGGGAGCATC AGGGGCTTAT CCACTAACCT CACCGCCTTC TTCATACCAC 
AATACAGTTA CGCCTTCTCC ATCAATGATG CACTCTCAGT CTCCAGGAAA TTTGCAAAGT 
TCACCTAGTG GTGTACTTAG AGCGCCTTCT CCTGCATCCT TTGGCCCCAC CCCTTCTCCG 
TCTTCCCTTG GAATTTCAAT GGTTCCAACC TCTAACTTTG CAAGTCCCCA TGGTTCTCAG 
TCAATGCCCA CAAATATGCC TCCTCCGCGT AAGCTACCTC AGCGATCTTG GGCCGCATCG 
ATTCCTACAA TACTCACTCA CAGTGCCTTG CATGTGCTTT TACTGCCTTC TCCTACACCT 
GGATTAGCCC CAGCATTGGC AGGCAGCTAC CTGTGTTCCC CTCTTGAAAG GTTCTTGGGA 
TCTGTCAATA TGAGACGACA CCTGCAAAGG ATCATTCAGC AGGAAACGTT AATAAATTCC 
AATGAACCAG GGGTGATAAT GTTCAAAACA GATGTATTAA AGTGCCGTGT GGCTCTTAAC 
CCAACTACCA ACCAAACTTT GCAACTAAAA GTTACACCTG AGAATGCTGG ACAGTGGAAG 
CCAGAAGAGC TTCAAGTTTT AGAGAGGTTC TTTGAGACCA GGGTTGCAGG ACCTCCATTC 
AAGGCAAATA CTTTGATAGC TTTCACCAAG TTATTAGGAG CACCTGCGCA TATCCTCAGA 
GACTGTGTGC ATATAATGAA GCTAGAACTG TTTCCAGATC AGACAACCCA ACTGAAATGG 
AATGTGCAGT TCTGCCTAAC CATCCCACCT AGTGCACCAC CAATAGCTCC ATCGGGAACT 
CCTGCTGTAG TACTGAAATC TAAAATGTTG TTTTTTCTTC AGCTAACTCA AAAGACTTCA 
GCTCCAACAG ATGCTTTCAG TATTATTGTT CCTATTATTT ATGATATGGC ATCAGGAACA 
ACCCAGCAAG CAGAAATCCC TCGGCAGCAG AACTCTTCAG TAACGGCTCC CTTAACAGTT 
AGCAATATTC TGAAAAGGTT TGCTGAGATG AATCCACCAC GGCCAGGTGA ATGTACAATA 
TTTGCTGCAG TACGAGATTT AATGGCCAAT CTCACATTGC CTCCCGGTGG TCGACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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med14 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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