nckap1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004917909.3	XP_004917966.1	nck-associated protein 1 isoform X1
XM_002932013.4	XP_002932059.1	nck-associated protein 1 isoform X3
XM_002932014.4	XP_002932060.1	nck-associated protein 1 isoform X4
XM_004917910.4	XP_004917967.1	nck-associated protein 1 isoform X2
XM_002932013.5	XP_002932059.1	nck-associated protein 1 isoform X3
XM_004917910.3	XP_004917967.1	nck-associated protein 1 isoform X2
XM_004917911.3	XP_004917968.1	nck-associated protein 1 isoform X5
XM_004917909.4	XP_004917966.1	nck-associated protein 1 isoform X1
XM_002932014.5	XP_002932060.1	nck-associated protein 1 isoform X4
XM_004917911.4	XP_004917968.1	nck-associated protein 1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa16222	XM_004917909.4 Latest version!	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa16223	XM_004917910.4 Latest version!	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa16224	XM_002932013.5 Latest version!	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa16225	XM_002932014.5 Latest version!	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa16226	XM_004917911.4 Latest version!	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa16224	XM_002932013.4	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa16223	XM_004917910.3	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa16222	XM_004917909.3	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa16225	XM_002932014.4	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa16226	XM_004917911.3	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

nckap1 ( XM_004917909.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917966.1 Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa16222
Clone ID Related Accession (Same CDS sequence)	XM_004917909.3 , XM_004917909.4
Accession Version	XM_004917909.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917909.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGCA AGGACAAGTC 
TGGAAGGCAT GTGGAGACCC CAAAGCAAAG CCTTCTTATC TGATCGACAA AAATCTTGAA 
TCTGCTGTCA AGTTTATTGT CCGGAAATTT CCAGCAGTTG AAACCCGCAA TAATAATCAA 
CAGCTTGCAC AACTTCAAAA AGAGAAATCT GAAATTTTGA AAAACCTGGC ACTGTACTAC 
TTTACCTTTG TTGATGTTAT GGAATTTAAG GATCATGTAT GTGAGCTGCT AAACACTATT 
GATGTCTGCC AGGTCTTCTT TGATATTACG GTTAACTTTG ATTTAACAAA GAACTATCTA 
GATCTGATTG TAACCTACAC AACACTAATG ATAATGCTTT CTCGGATTGA GGAGAGAAAA 
GCAATTATAG GACTCTACAA CTACTCTCAT GAAATGACCC ATGGGGCAAG TGATCGGGAA 
TATCCTCGGC TTGGACAGAT GATCGTTGAC TATGAAAACC CTTTGAAGAA AATGATGGAG 
GAGTTTGTTC CCCATAGTAA GTCTCTATCA GATGCCCTAA TATCACTGCA AATGGTTTAT 
CCACGAAGGA ATCTTTCAGC TGAGCAGTGG CGAAATGCTC AACTTCTAAG TCTCATCAGT 
GCTCCTAGTA CAATGCTAAA CCCAGCACAA TCGGACACGA TGCCATGCGA GTACCTTTCG 
CTTGATGCAA TGGAGAAGTG GATTATTTTT GGATTTATTT TGTGCCATGG AATACTCAAC 
ACTGATGCTA CAGCATTGAA TCTTTGGAAG CTCGCCCTTC AGAGCAGTTC ATGTCTCTGT 
CTTTACAGAG ATGAAGTTTT TCATATCCAC AAGGCAGCAG AAGATCTGTT CGTCAACATT 
AGAGGCTACA ACAAAAGGAT AAATGACATC AGGGAGTGTA AAGAGAATGC AGTATCACAT 
GCAGGAGCAA CACACAGAGA AAGGCGCAAA TTTTTAAGGT CTGCTTTGAA AGAACTTGCT 
ACTGTACTCG CTGATCAGCC AGGCCTTCTG GGTCCCAAAG CACTTTTTGT ATTCATGGCA 
TTATCGTTTG CCCGTGATGA AATAATATGG CTACTTCGTC ATGCAGACAA CATTCCAAAG 
AAGGTCGCAG ATGACTTCAT GGATAAGCAC ATCGCTGAGC TCATATTTTA TATGGAAGAA 
CTGCGAGCGC ATGTACGGAA GTATGGGCCT GTGATGCAGA GATACTATGT GCAGTACCTG 
TCTGGCTTTG ATGCAGTTGT ACTAAATGAG CTAGTGCAGA ATCTTTCTGT GTGCCCTGAG 
GATGAATCAA TTATAATGTC TTCATTCGTA AATACAATGA CCTCCTTGAG TGTAAAACAA 
GTTGAAGACG GAGAGGTTTT TGACTTCAGA GGAATGAGAT TGGATTGGTT CAGATTGCAG 
GCCTATACCA GTGTTTCCAA AGCTTCATTG AGTCTTGCTG ACCACAGAGA ACTTGGAAAA 
ATGATGAACA CTATAATATT CCATACCAAA ATGGTGGATT CCTTAGTGGA GATGTTGGTG 
GAGACATCAG ACCTTTCCAT ATTTTGCTTT TACAGCCGAG CTTTTGAGAA GATGTTTCAG 
CAGTGTTTGG AGCTGCCATC CCAATCAAGA TATTCAATCT CCTTTCCTCT TATATGCACT 
CACTTTATGA GCTGCACTCA CGAACTTTGC CCTGAAGAGG TAATTTTTAT TAATTCCCAG 
CGGCATCACA TTGGAGACCG CAGTTTGTCA CTATGTAACA TGTTCTTGGA TGAAATGGCA 
AAGCAAGCTC GAAATCTAAT TACAGACATC TGCACGGAAC AGTGTACCCT TAGTGATCAG 
TTGCTGCCAA AGCACTGTGC CAAAACAATC AGTCAAGCGG TGAACAAAAA GTCAAAAAAG 
CAGACCGGAA AGAAAGGAGA ACCAGAAAGG GAAAAACCTG GAGTTGAAAG CATGAGAAAG 
AATAGACTTG TGGTAACAAA CCTGGATAAA TTGCACACTG CACTTTCAGA GCTCTGTTTC 
TCTATCAACT ATGCACCAAA TATGGTTGTA TGGGAACACA CATTTACCCC AAGAGAGTAT 
TTGACGTCGC ACTTGGAAAT CCGCTTTACC AAGTCTATTG TTGGCATGAC CATGTACAAT 
CAAGTCACTC AAGAGATTGC TAAACCTTCT GAGTTGTTAA CAAGTGTAAG AGCCTATATG 
ACTGTACTCC AATCAATAGA AAACTACGTG CAGATTGATA TCACAAGGGT ATTCAACAAT 
GTTCTACTTC AGCAAACACA ACATTTAGAC AGCCATGGAG AGCCAACAAT CACCAGTTTA 
TACACTAATT GGTATTTGGA AACACTACTG AGGCAAGTAA GCAATGGACA TATAGCATAT 
TTCCCAGCAA TGAAAGCTTT TGTAAACCTG CCAACTGAGA ATGAGCTGAC GTTTAATGCA 
GAAGAGTATT CCGACATCTC TGAAATGAGG GCATTGTCTG AGCTGCTGGG ACCATATGGC 
ATGAAGTTTC TCAGTGAAAG CCTCATGTGG CATATTTCCT CTCAGGTGGC TGAGCTGAAG 
AAACTTGTTG TGGAAAATGT GGAGGTCTTA ACCCAGATGA GAACAAGTTT TGATAAACCA 
GAGCAGATGG CAGCCCTTTT CAAAAGGCTT ACATCCGTTG ACAGTGTTTT GAAGAGGATG 
ACCATTATTG GAGTTATATT GTCTTTCCGA TCTCTTGCAC AGGAAGCACT AAGAGATGTT 
TTGTCTTACC ACATTCCTTT TCTTGTAAGC TCTGTTGAAG ACTTTAAGGA TCACATTCCT 
AGAGAGACAG ACATGAAGGT GGCTATGAAT GTGTATGAGT TATCATCAGC AGCTGGATTG 
CCATGTGAAA TAGACCCTGC CTTAGTTGTG GCACTGTCAT CTCAAAAATC TGAAACCATC 
AGCCCAGAGG AAGAATACAA AATAGCCTGT CTGCTTATGG TGTTTGTGGC TGTTTCTTTG 
CCAACATTGG CTAGTAACGT CATGTCGCAA TACAGCCCTG CAATTGAAGG TCATTGCAAC 
AACATACACT GCTTGGCAAA GGCAATTAAC CAAATTGCAG CAGCACTATT TACCATTCAC 
AAAGGGAGCA TCGAAGATCG TCTGAAAGAG TTCTTGGCGC TTGCATCCTC CAGCCTGCTA 
AAGATTGGCC AAGAGACTGA CAAAACTACT ACAAGAAACA GGGAATCTGT TTATTTGCTG 
CTAGATATGA TTGTTCAAGA ATCACCATTC CTGACCATGG ATCTGCTGGA GTCCTGCTTC 
CCATACGTCT TACTGAGAAA TGCATACCAT GCGGTTTACA AACAGAGTGT CACATCCTCT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917966.1
CDS	283..3708
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIVTYTTLMIMLSRIEERKAIIGLYNYSHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSAEQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLCLYRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKENAVSHAGATHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIPKKVADDFMDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLICTHFMSCTHELCPEEVIFINSQRHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYAPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQVTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPEQMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSETISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_004917909.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917909.4

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGCA AGGACAAGTC 
TGGAAGGCAT GTGGAGACCC CAAAGCAAAG CCTTCTTATC TGATCGACAA AAATCTTGAA 
TCTGCTGTCA AGTTTATTGT CCGGAAATTT CCAGCAGTTG AAACCCGCAA TAATAATCAA 
CAGCTTGCAC AACTTCAAAA AGAGAAATCT GAAATTTTGA AAAACCTGGC ACTGTACTAC 
TTTACCTTTG TTGATGTTAT GGAATTTAAG GATCATGTAT GTGAGCTGCT AAACACTATT 
GATGTCTGCC AGGTCTTCTT TGATATTACG GTTAACTTTG ATTTAACAAA GAACTATCTA 
GATCTGATTG TAACCTACAC AACACTAATG ATAATGCTTT CTCGGATTGA GGAGAGAAAA 
GCAATTATAG GACTCTACAA CTACTCTCAT GAAATGACCC ATGGGGCAAG TGATCGGGAA 
TATCCTCGGC TTGGACAGAT GATCGTTGAC TATGAAAACC CTTTGAAGAA AATGATGGAG 
GAGTTTGTTC CCCATAGTAA GTCTCTATCA GATGCCCTAA TATCACTGCA AATGGTTTAT 
CCACGAAGGA ATCTTTCAGC TGAGCAGTGG CGAAATGCTC AACTTCTAAG TCTCATCAGT 
GCTCCTAGTA CAATGCTAAA CCCAGCACAA TCGGACACGA TGCCATGCGA GTACCTTTCG 
CTTGATGCAA TGGAGAAGTG GATTATTTTT GGATTTATTT TGTGCCATGG AATACTCAAC 
ACTGATGCTA CAGCATTGAA TCTTTGGAAG CTCGCCCTTC AGAGCAGTTC ATGTCTCTGT 
CTTTACAGAG ATGAAGTTTT TCATATCCAC AAGGCAGCAG AAGATCTGTT CGTCAACATT 
AGAGGCTACA ACAAAAGGAT AAATGACATC AGGGAGTGTA AAGAGAATGC AGTATCACAT 
GCAGGAGCAA CACACAGAGA AAGGCGCAAA TTTTTAAGGT CTGCTTTGAA AGAACTTGCT 
ACTGTACTCG CTGATCAGCC AGGCCTTCTG GGTCCCAAAG CACTTTTTGT ATTCATGGCA 
TTATCGTTTG CCCGTGATGA AATAATATGG CTACTTCGTC ATGCAGACAA CATTCCAAAG 
AAGGTCGCAG ATGACTTCAT GGATAAGCAC ATCGCTGAGC TCATATTTTA TATGGAAGAA 
CTGCGAGCGC ATGTACGGAA GTATGGGCCT GTGATGCAGA GATACTATGT GCAGTACCTG 
TCTGGCTTTG ATGCAGTTGT ACTAAATGAG CTAGTGCAGA ATCTTTCTGT GTGCCCTGAG 
GATGAATCAA TTATAATGTC TTCATTCGTA AATACAATGA CCTCCTTGAG TGTAAAACAA 
GTTGAAGACG GAGAGGTTTT TGACTTCAGA GGAATGAGAT TGGATTGGTT CAGATTGCAG 
GCCTATACCA GTGTTTCCAA AGCTTCATTG AGTCTTGCTG ACCACAGAGA ACTTGGAAAA 
ATGATGAACA CTATAATATT CCATACCAAA ATGGTGGATT CCTTAGTGGA GATGTTGGTG 
GAGACATCAG ACCTTTCCAT ATTTTGCTTT TACAGCCGAG CTTTTGAGAA GATGTTTCAG 
CAGTGTTTGG AGCTGCCATC CCAATCAAGA TATTCAATCT CCTTTCCTCT TATATGCACT 
CACTTTATGA GCTGCACTCA CGAACTTTGC CCTGAAGAGG TAATTTTTAT TAATTCCCAG 
CGGCATCACA TTGGAGACCG CAGTTTGTCA CTATGTAACA TGTTCTTGGA TGAAATGGCA 
AAGCAAGCTC GAAATCTAAT TACAGACATC TGCACGGAAC AGTGTACCCT TAGTGATCAG 
TTGCTGCCAA AGCACTGTGC CAAAACAATC AGTCAAGCGG TGAACAAAAA GTCAAAAAAG 
CAGACCGGAA AGAAAGGAGA ACCAGAAAGG GAAAAACCTG GAGTTGAAAG CATGAGAAAG 
AATAGACTTG TGGTAACAAA CCTGGATAAA TTGCACACTG CACTTTCAGA GCTCTGTTTC 
TCTATCAACT ATGCACCAAA TATGGTTGTA TGGGAACACA CATTTACCCC AAGAGAGTAT 
TTGACGTCGC ACTTGGAAAT CCGCTTTACC AAGTCTATTG TTGGCATGAC CATGTACAAT 
CAAGTCACTC AAGAGATTGC TAAACCTTCT GAGTTGTTAA CAAGTGTAAG AGCCTATATG 
ACTGTACTCC AATCAATAGA AAACTACGTG CAGATTGATA TCACAAGGGT ATTCAACAAT 
GTTCTACTTC AGCAAACACA ACATTTAGAC AGCCATGGAG AGCCAACAAT CACCAGTTTA 
TACACTAATT GGTATTTGGA AACACTACTG AGGCAAGTAA GCAATGGACA TATAGCATAT 
TTCCCAGCAA TGAAAGCTTT TGTAAACCTG CCAACTGAGA ATGAGCTGAC GTTTAATGCA 
GAAGAGTATT CCGACATCTC TGAAATGAGG GCATTGTCTG AGCTGCTGGG ACCATATGGC 
ATGAAGTTTC TCAGTGAAAG CCTCATGTGG CATATTTCCT CTCAGGTGGC TGAGCTGAAG 
AAACTTGTTG TGGAAAATGT GGAGGTCTTA ACCCAGATGA GAACAAGTTT TGATAAACCA 
GAGCAGATGG CAGCCCTTTT CAAAAGGCTT ACATCCGTTG ACAGTGTTTT GAAGAGGATG 
ACCATTATTG GAGTTATATT GTCTTTCCGA TCTCTTGCAC AGGAAGCACT AAGAGATGTT 
TTGTCTTACC ACATTCCTTT TCTTGTAAGC TCTGTTGAAG ACTTTAAGGA TCACATTCCT 
AGAGAGACAG ACATGAAGGT GGCTATGAAT GTGTATGAGT TATCATCAGC AGCTGGATTG 
CCATGTGAAA TAGACCCTGC CTTAGTTGTG GCACTGTCAT CTCAAAAATC TGAAACCATC 
AGCCCAGAGG AAGAATACAA AATAGCCTGT CTGCTTATGG TGTTTGTGGC TGTTTCTTTG 
CCAACATTGG CTAGTAACGT CATGTCGCAA TACAGCCCTG CAATTGAAGG TCATTGCAAC 
AACATACACT GCTTGGCAAA GGCAATTAAC CAAATTGCAG CAGCACTATT TACCATTCAC 
AAAGGGAGCA TCGAAGATCG TCTGAAAGAG TTCTTGGCGC TTGCATCCTC CAGCCTGCTA 
AAGATTGGCC AAGAGACTGA CAAAACTACT ACAAGAAACA GGGAATCTGT TTATTTGCTG 
CTAGATATGA TTGTTCAAGA ATCACCATTC CTGACCATGG ATCTGCTGGA GTCCTGCTTC 
CCATACGTCT TACTGAGAAA TGCATACCAT GCGGTTTACA AACAGAGTGT CACATCCTCT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap1 ( XM_004917910.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917967.1 Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16223
Clone ID Related Accession (Same CDS sequence)	XM_004917910.4 , XM_004917910.3
Accession Version	XM_004917910.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917910.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAAGCAA AGCCTTCTTA TCTGATCGAC AAAAATCTTG AATCTGCTGT CAAGTTTATT 
GTCCGGAAAT TTCCAGCAGT TGAAACCCGC AATAATAATC AACAGCTTGC ACAACTTCAA 
AAAGAGAAAT CTGAAATTTT GAAAAACCTG GCACTGTACT ACTTTACCTT TGTTGATGTT 
ATGGAATTTA AGGATCATGT ATGTGAGCTG CTAAACACTA TTGATGTCTG CCAGGTCTTC 
TTTGATATTA CGGTTAACTT TGATTTAACA AAGAACTATC TAGATCTGAT TGTAACCTAC 
ACAACACTAA TGATAATGCT TTCTCGGATT GAGGAGAGAA AAGCAATTAT AGGACTCTAC 
AACTACTCTC ATGAAATGAC CCATGGGGCA AGTGATCGGG AATATCCTCG GCTTGGACAG 
ATGATCGTTG ACTATGAAAA CCCTTTGAAG AAAATGATGG AGGAGTTTGT TCCCCATAGT 
AAGTCTCTAT CAGATGCCCT AATATCACTG CAAATGGTTT ATCCACGAAG GAATCTTTCA 
GCTGAGCAGT GGCGAAATGC TCAACTTCTA AGTCTCATCA GTGCTCCTAG TACAATGCTA 
AACCCAGCAC AATCGGACAC GATGCCATGC GAGTACCTTT CGCTTGATGC AATGGAGAAG 
TGGATTATTT TTGGATTTAT TTTGTGCCAT GGAATACTCA ACACTGATGC TACAGCATTG 
AATCTTTGGA AGCTCGCCCT TCAGAGCAGT TCATGTCTCT GTCTTTACAG AGATGAAGTT 
TTTCATATCC ACAAGGCAGC AGAAGATCTG TTCGTCAACA TTAGAGGCTA CAACAAAAGG 
ATAAATGACA TCAGGGAGTG TAAAGAGAAT GCAGTATCAC ATGCAGGAGC AACACACAGA 
GAAAGGCGCA AATTTTTAAG GTCTGCTTTG AAAGAACTTG CTACTGTACT CGCTGATCAG 
CCAGGCCTTC TGGGTCCCAA AGCACTTTTT GTATTCATGG CATTATCGTT TGCCCGTGAT 
GAAATAATAT GGCTACTTCG TCATGCAGAC AACATTCCAA AGAAGGTCGC AGATGACTTC 
ATGGATAAGC ACATCGCTGA GCTCATATTT TATATGGAAG AACTGCGAGC GCATGTACGG 
AAGTATGGGC CTGTGATGCA GAGATACTAT GTGCAGTACC TGTCTGGCTT TGATGCAGTT 
GTACTAAATG AGCTAGTGCA GAATCTTTCT GTGTGCCCTG AGGATGAATC AATTATAATG 
TCTTCATTCG TAAATACAAT GACCTCCTTG AGTGTAAAAC AAGTTGAAGA CGGAGAGGTT 
TTTGACTTCA GAGGAATGAG ATTGGATTGG TTCAGATTGC AGGCCTATAC CAGTGTTTCC 
AAAGCTTCAT TGAGTCTTGC TGACCACAGA GAACTTGGAA AAATGATGAA CACTATAATA 
TTCCATACCA AAATGGTGGA TTCCTTAGTG GAGATGTTGG TGGAGACATC AGACCTTTCC 
ATATTTTGCT TTTACAGCCG AGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTGCCA 
TCCCAATCAA GATATTCAAT CTCCTTTCCT CTTATATGCA CTCACTTTAT GAGCTGCACT 
CACGAACTTT GCCCTGAAGA GGTAATTTTT ATTAATTCCC AGCGGCATCA CATTGGAGAC 
CGCAGTTTGT CACTATGTAA CATGTTCTTG GATGAAATGG CAAAGCAAGC TCGAAATCTA 
ATTACAGACA TCTGCACGGA ACAGTGTACC CTTAGTGATC AGTTGCTGCC AAAGCACTGT 
GCCAAAACAA TCAGTCAAGC GGTGAACAAA AAGTCAAAAA AGCAGACCGG AAAGAAAGGA 
GAACCAGAAA GGGAAAAACC TGGAGTTGAA AGCATGAGAA AGAATAGACT TGTGGTAACA 
AACCTGGATA AATTGCACAC TGCACTTTCA GAGCTCTGTT TCTCTATCAA CTATGCACCA 
AATATGGTTG TATGGGAACA CACATTTACC CCAAGAGAGT ATTTGACGTC GCACTTGGAA 
ATCCGCTTTA CCAAGTCTAT TGTTGGCATG ACCATGTACA ATCAAGTCAC TCAAGAGATT 
GCTAAACCTT CTGAGTTGTT AACAAGTGTA AGAGCCTATA TGACTGTACT CCAATCAATA 
GAAAACTACG TGCAGATTGA TATCACAAGG GTATTCAACA ATGTTCTACT TCAGCAAACA 
CAACATTTAG ACAGCCATGG AGAGCCAACA ATCACCAGTT TATACACTAA TTGGTATTTG 
GAAACACTAC TGAGGCAAGT AAGCAATGGA CATATAGCAT ATTTCCCAGC AATGAAAGCT 
TTTGTAAACC TGCCAACTGA GAATGAGCTG ACGTTTAATG CAGAAGAGTA TTCCGACATC 
TCTGAAATGA GGGCATTGTC TGAGCTGCTG GGACCATATG GCATGAAGTT TCTCAGTGAA 
AGCCTCATGT GGCATATTTC CTCTCAGGTG GCTGAGCTGA AGAAACTTGT TGTGGAAAAT 
GTGGAGGTCT TAACCCAGAT GAGAACAAGT TTTGATAAAC CAGAGCAGAT GGCAGCCCTT 
TTCAAAAGGC TTACATCCGT TGACAGTGTT TTGAAGAGGA TGACCATTAT TGGAGTTATA 
TTGTCTTTCC GATCTCTTGC ACAGGAAGCA CTAAGAGATG TTTTGTCTTA CCACATTCCT 
TTTCTTGTAA GCTCTGTTGA AGACTTTAAG GATCACATTC CTAGAGAGAC AGACATGAAG 
GTGGCTATGA ATGTGTATGA GTTATCATCA GCAGCTGGAT TGCCATGTGA AATAGACCCT 
GCCTTAGTTG TGGCACTGTC ATCTCAAAAA TCTGAAACCA TCAGCCCAGA GGAAGAATAC 
AAAATAGCCT GTCTGCTTAT GGTGTTTGTG GCTGTTTCTT TGCCAACATT GGCTAGTAAC 
GTCATGTCGC AATACAGCCC TGCAATTGAA GGTCATTGCA ACAACATACA CTGCTTGGCA 
AAGGCAATTA ACCAAATTGC AGCAGCACTA TTTACCATTC ACAAAGGGAG CATCGAAGAT 
CGTCTGAAAG AGTTCTTGGC GCTTGCATCC TCCAGCCTGC TAAAGATTGG CCAAGAGACT 
GACAAAACTA CTACAAGAAA CAGGGAATCT GTTTATTTGC TGCTAGATAT GATTGTTCAA 
GAATCACCAT TCCTGACCAT GGATCTGCTG GAGTCCTGCT TCCCATACGT CTTACTGAGA 
AATGCATACC ATGCGGTTTA CAAACAGAGT GTCACATCCT CTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917967.1
CDS	282..3689
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIVTYTTLMIMLSRIEERKAIIGLYNYSHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSAEQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLCLYRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKENAVSHAGATHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIPKKVADDFMDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLICTHFMSCTHELCPEEVIFINSQRHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYAPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQVTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPEQMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSETISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_004917910.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917910.4

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAAGCAA AGCCTTCTTA TCTGATCGAC AAAAATCTTG AATCTGCTGT CAAGTTTATT 
GTCCGGAAAT TTCCAGCAGT TGAAACCCGC AATAATAATC AACAGCTTGC ACAACTTCAA 
AAAGAGAAAT CTGAAATTTT GAAAAACCTG GCACTGTACT ACTTTACCTT TGTTGATGTT 
ATGGAATTTA AGGATCATGT ATGTGAGCTG CTAAACACTA TTGATGTCTG CCAGGTCTTC 
TTTGATATTA CGGTTAACTT TGATTTAACA AAGAACTATC TAGATCTGAT TGTAACCTAC 
ACAACACTAA TGATAATGCT TTCTCGGATT GAGGAGAGAA AAGCAATTAT AGGACTCTAC 
AACTACTCTC ATGAAATGAC CCATGGGGCA AGTGATCGGG AATATCCTCG GCTTGGACAG 
ATGATCGTTG ACTATGAAAA CCCTTTGAAG AAAATGATGG AGGAGTTTGT TCCCCATAGT 
AAGTCTCTAT CAGATGCCCT AATATCACTG CAAATGGTTT ATCCACGAAG GAATCTTTCA 
GCTGAGCAGT GGCGAAATGC TCAACTTCTA AGTCTCATCA GTGCTCCTAG TACAATGCTA 
AACCCAGCAC AATCGGACAC GATGCCATGC GAGTACCTTT CGCTTGATGC AATGGAGAAG 
TGGATTATTT TTGGATTTAT TTTGTGCCAT GGAATACTCA ACACTGATGC TACAGCATTG 
AATCTTTGGA AGCTCGCCCT TCAGAGCAGT TCATGTCTCT GTCTTTACAG AGATGAAGTT 
TTTCATATCC ACAAGGCAGC AGAAGATCTG TTCGTCAACA TTAGAGGCTA CAACAAAAGG 
ATAAATGACA TCAGGGAGTG TAAAGAGAAT GCAGTATCAC ATGCAGGAGC AACACACAGA 
GAAAGGCGCA AATTTTTAAG GTCTGCTTTG AAAGAACTTG CTACTGTACT CGCTGATCAG 
CCAGGCCTTC TGGGTCCCAA AGCACTTTTT GTATTCATGG CATTATCGTT TGCCCGTGAT 
GAAATAATAT GGCTACTTCG TCATGCAGAC AACATTCCAA AGAAGGTCGC AGATGACTTC 
ATGGATAAGC ACATCGCTGA GCTCATATTT TATATGGAAG AACTGCGAGC GCATGTACGG 
AAGTATGGGC CTGTGATGCA GAGATACTAT GTGCAGTACC TGTCTGGCTT TGATGCAGTT 
GTACTAAATG AGCTAGTGCA GAATCTTTCT GTGTGCCCTG AGGATGAATC AATTATAATG 
TCTTCATTCG TAAATACAAT GACCTCCTTG AGTGTAAAAC AAGTTGAAGA CGGAGAGGTT 
TTTGACTTCA GAGGAATGAG ATTGGATTGG TTCAGATTGC AGGCCTATAC CAGTGTTTCC 
AAAGCTTCAT TGAGTCTTGC TGACCACAGA GAACTTGGAA AAATGATGAA CACTATAATA 
TTCCATACCA AAATGGTGGA TTCCTTAGTG GAGATGTTGG TGGAGACATC AGACCTTTCC 
ATATTTTGCT TTTACAGCCG AGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTGCCA 
TCCCAATCAA GATATTCAAT CTCCTTTCCT CTTATATGCA CTCACTTTAT GAGCTGCACT 
CACGAACTTT GCCCTGAAGA GGTAATTTTT ATTAATTCCC AGCGGCATCA CATTGGAGAC 
CGCAGTTTGT CACTATGTAA CATGTTCTTG GATGAAATGG CAAAGCAAGC TCGAAATCTA 
ATTACAGACA TCTGCACGGA ACAGTGTACC CTTAGTGATC AGTTGCTGCC AAAGCACTGT 
GCCAAAACAA TCAGTCAAGC GGTGAACAAA AAGTCAAAAA AGCAGACCGG AAAGAAAGGA 
GAACCAGAAA GGGAAAAACC TGGAGTTGAA AGCATGAGAA AGAATAGACT TGTGGTAACA 
AACCTGGATA AATTGCACAC TGCACTTTCA GAGCTCTGTT TCTCTATCAA CTATGCACCA 
AATATGGTTG TATGGGAACA CACATTTACC CCAAGAGAGT ATTTGACGTC GCACTTGGAA 
ATCCGCTTTA CCAAGTCTAT TGTTGGCATG ACCATGTACA ATCAAGTCAC TCAAGAGATT 
GCTAAACCTT CTGAGTTGTT AACAAGTGTA AGAGCCTATA TGACTGTACT CCAATCAATA 
GAAAACTACG TGCAGATTGA TATCACAAGG GTATTCAACA ATGTTCTACT TCAGCAAACA 
CAACATTTAG ACAGCCATGG AGAGCCAACA ATCACCAGTT TATACACTAA TTGGTATTTG 
GAAACACTAC TGAGGCAAGT AAGCAATGGA CATATAGCAT ATTTCCCAGC AATGAAAGCT 
TTTGTAAACC TGCCAACTGA GAATGAGCTG ACGTTTAATG CAGAAGAGTA TTCCGACATC 
TCTGAAATGA GGGCATTGTC TGAGCTGCTG GGACCATATG GCATGAAGTT TCTCAGTGAA 
AGCCTCATGT GGCATATTTC CTCTCAGGTG GCTGAGCTGA AGAAACTTGT TGTGGAAAAT 
GTGGAGGTCT TAACCCAGAT GAGAACAAGT TTTGATAAAC CAGAGCAGAT GGCAGCCCTT 
TTCAAAAGGC TTACATCCGT TGACAGTGTT TTGAAGAGGA TGACCATTAT TGGAGTTATA 
TTGTCTTTCC GATCTCTTGC ACAGGAAGCA CTAAGAGATG TTTTGTCTTA CCACATTCCT 
TTTCTTGTAA GCTCTGTTGA AGACTTTAAG GATCACATTC CTAGAGAGAC AGACATGAAG 
GTGGCTATGA ATGTGTATGA GTTATCATCA GCAGCTGGAT TGCCATGTGA AATAGACCCT 
GCCTTAGTTG TGGCACTGTC ATCTCAAAAA TCTGAAACCA TCAGCCCAGA GGAAGAATAC 
AAAATAGCCT GTCTGCTTAT GGTGTTTGTG GCTGTTTCTT TGCCAACATT GGCTAGTAAC 
GTCATGTCGC AATACAGCCC TGCAATTGAA GGTCATTGCA ACAACATACA CTGCTTGGCA 
AAGGCAATTA ACCAAATTGC AGCAGCACTA TTTACCATTC ACAAAGGGAG CATCGAAGAT 
CGTCTGAAAG AGTTCTTGGC GCTTGCATCC TCCAGCCTGC TAAAGATTGG CCAAGAGACT 
GACAAAACTA CTACAAGAAA CAGGGAATCT GTTTATTTGC TGCTAGATAT GATTGTTCAA 
GAATCACCAT TCCTGACCAT GGATCTGCTG GAGTCCTGCT TCCCATACGT CTTACTGAGA 
AATGCATACC ATGCGGTTTA CAAACAGAGT GTCACATCCT CTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap1 ( XM_002932013.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932059.1 Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa16224
Clone ID Related Accession (Same CDS sequence)	XM_002932013.4 , XM_002932013.5
Accession Version	XM_002932013.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932013.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGCA AGGACAAGTC 
TGGAAGGCAT GTGGAGACCC CAAAGCAAAG CCTTCTTATC TGATCGACAA AAATCTTGAA 
TCTGCTGTCA AGTTTATTGT CCGGAAATTT CCAGCAGTTG AAACCCGCAA TAATAATCAA 
CAGCTTGCAC AACTTCAAAA AGAGAAATCT GAAATTTTGA AAAACCTGGC ACTGTACTAC 
TTTACCTTTG TTGATGTTAT GGAATTTAAG GATCATGTAT GTGAGCTGCT AAACACTATT 
GATGTCTGCC AGGTCTTCTT TGATATTACG GTTAACTTTG ATTTAACAAA GAACTATCTA 
GATCTGATTG TAACCTACAC AACACTAATG ATAATGCTTT CTCGGATTGA GGAGAGAAAA 
GCAATTATAG GACTCTACAA CTACTCTCAT GAAATGACCC ATGGGGCAAG TGATCGGGAA 
TATCCTCGGC TTGGACAGAT GATCGTTGAC TATGAAAACC CTTTGAAGAA AATGATGGAG 
GAGTTTGTTC CCCATAGTAA GTCTCTATCA GATGCCCTAA TATCACTGCA AATGGTTTAT 
CCACGAAGGA ATCTTTCAGC TGAGCAGTGG CGAAATGCTC AACTTCTAAG TCTCATCAGT 
GCTCCTAGTA CAATGCTAAA CCCAGCACAA TCGGACACGA TGCCATGCGA GTACCTTTCG 
CTTGATGCAA TGGAGAAGTG GATTATTTTT GGATTTATTT TGTGCCATGG AATACTCAAC 
ACTGATGCTA CAGCATTGAA TCTTTGGAAG CTCGCCCTTC AGAGCAGTTC ATGTCTCTGT 
CTTTACAGAG ATGAAGTTTT TCATATCCAC AAGGCAGCAG AAGATCTGTT CGTCAACATT 
AGAGGCTACA ACAAAAGGAT AAATGACATC AGGGAGTGTA AAGAGAATGC AGTATCACAT 
GCAGGAGCAA CACACAGAGA AAGGCGCAAA TTTTTAAGGT CTGCTTTGAA AGAACTTGCT 
ACTGTACTCG CTGATCAGCC AGGCCTTCTG GGTCCCAAAG CACTTTTTGT ATTCATGGCA 
TTATCGTTTG CCCGTGATGA AATAATATGG CTACTTCGTC ATGCAGACAA CATTCCAAAG 
AAGGTCGCAG ATGACTTCAT GGATAAGCAC ATCGCTGAGC TCATATTTTA TATGGAAGAA 
CTGCGAGCGC ATGTACGGAA GTATGGGCCT GTGATGCAGA GATACTATGT GCAGTACCTG 
TCTGGCTTTG ATGCAGTTGT ACTAAATGAG CTAGTGCAGA ATCTTTCTGT GTGCCCTGAG 
GATGAATCAA TTATAATGTC TTCATTCGTA AATACAATGA CCTCCTTGAG TGTAAAACAA 
GTTGAAGACG GAGAGGTTTT TGACTTCAGA GGAATGAGAT TGGATTGGTT CAGATTGCAG 
GCCTATACCA GTGTTTCCAA AGCTTCATTG AGTCTTGCTG ACCACAGAGA ACTTGGAAAA 
ATGATGAACA CTATAATATT CCATACCAAA ATGGTGGATT CCTTAGTGGA GATGTTGGTG 
GAGACATCAG ACCTTTCCAT ATTTTGCTTT TACAGCCGAG CTTTTGAGAA GATGTTTCAG 
CAGTGTTTGG AGCTGCCATC CCAATCAAGA TATTCAATCT CCTTTCCTCT TATATGCACT 
CACTTTATGA GCTGCACTCA CGAACTTTGC CCTGAAGAGC GGCATCACAT TGGAGACCGC 
AGTTTGTCAC TATGTAACAT GTTCTTGGAT GAAATGGCAA AGCAAGCTCG AAATCTAATT 
ACAGACATCT GCACGGAACA GTGTACCCTT AGTGATCAGT TGCTGCCAAA GCACTGTGCC 
AAAACAATCA GTCAAGCGGT GAACAAAAAG TCAAAAAAGC AGACCGGAAA GAAAGGAGAA 
CCAGAAAGGG AAAAACCTGG AGTTGAAAGC ATGAGAAAGA ATAGACTTGT GGTAACAAAC 
CTGGATAAAT TGCACACTGC ACTTTCAGAG CTCTGTTTCT CTATCAACTA TGCACCAAAT 
ATGGTTGTAT GGGAACACAC ATTTACCCCA AGAGAGTATT TGACGTCGCA CTTGGAAATC 
CGCTTTACCA AGTCTATTGT TGGCATGACC ATGTACAATC AAGTCACTCA AGAGATTGCT 
AAACCTTCTG AGTTGTTAAC AAGTGTAAGA GCCTATATGA CTGTACTCCA ATCAATAGAA 
AACTACGTGC AGATTGATAT CACAAGGGTA TTCAACAATG TTCTACTTCA GCAAACACAA 
CATTTAGACA GCCATGGAGA GCCAACAATC ACCAGTTTAT ACACTAATTG GTATTTGGAA 
ACACTACTGA GGCAAGTAAG CAATGGACAT ATAGCATATT TCCCAGCAAT GAAAGCTTTT 
GTAAACCTGC CAACTGAGAA TGAGCTGACG TTTAATGCAG AAGAGTATTC CGACATCTCT 
GAAATGAGGG CATTGTCTGA GCTGCTGGGA CCATATGGCA TGAAGTTTCT CAGTGAAAGC 
CTCATGTGGC ATATTTCCTC TCAGGTGGCT GAGCTGAAGA AACTTGTTGT GGAAAATGTG 
GAGGTCTTAA CCCAGATGAG AACAAGTTTT GATAAACCAG AGCAGATGGC AGCCCTTTTC 
AAAAGGCTTA CATCCGTTGA CAGTGTTTTG AAGAGGATGA CCATTATTGG AGTTATATTG 
TCTTTCCGAT CTCTTGCACA GGAAGCACTA AGAGATGTTT TGTCTTACCA CATTCCTTTT 
CTTGTAAGCT CTGTTGAAGA CTTTAAGGAT CACATTCCTA GAGAGACAGA CATGAAGGTG 
GCTATGAATG TGTATGAGTT ATCATCAGCA GCTGGATTGC CATGTGAAAT AGACCCTGCC 
TTAGTTGTGG CACTGTCATC TCAAAAATCT GAAACCATCA GCCCAGAGGA AGAATACAAA 
ATAGCCTGTC TGCTTATGGT GTTTGTGGCT GTTTCTTTGC CAACATTGGC TAGTAACGTC 
ATGTCGCAAT ACAGCCCTGC AATTGAAGGT CATTGCAACA ACATACACTG CTTGGCAAAG 
GCAATTAACC AAATTGCAGC AGCACTATTT ACCATTCACA AAGGGAGCAT CGAAGATCGT 
CTGAAAGAGT TCTTGGCGCT TGCATCCTCC AGCCTGCTAA AGATTGGCCA AGAGACTGAC 
AAAACTACTA CAAGAAACAG GGAATCTGTT TATTTGCTGC TAGATATGAT TGTTCAAGAA 
TCACCATTCC TGACCATGGA TCTGCTGGAG TCCTGCTTCC CATACGTCTT ACTGAGAAAT 
GCATACCATG CGGTTTACAA ACAGAGTGTC ACATCCTCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932059.1
CDS	282..3686
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIVTYTTLMIMLSRIEERKAIIGLYNYSHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSAEQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLCLYRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKENAVSHAGATHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIPKKVADDFMDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLICTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYAPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQVTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPEQMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSETISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_002932013.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932013.5

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGCA AGGACAAGTC 
TGGAAGGCAT GTGGAGACCC CAAAGCAAAG CCTTCTTATC TGATCGACAA AAATCTTGAA 
TCTGCTGTCA AGTTTATTGT CCGGAAATTT CCAGCAGTTG AAACCCGCAA TAATAATCAA 
CAGCTTGCAC AACTTCAAAA AGAGAAATCT GAAATTTTGA AAAACCTGGC ACTGTACTAC 
TTTACCTTTG TTGATGTTAT GGAATTTAAG GATCATGTAT GTGAGCTGCT AAACACTATT 
GATGTCTGCC AGGTCTTCTT TGATATTACG GTTAACTTTG ATTTAACAAA GAACTATCTA 
GATCTGATTG TAACCTACAC AACACTAATG ATAATGCTTT CTCGGATTGA GGAGAGAAAA 
GCAATTATAG GACTCTACAA CTACTCTCAT GAAATGACCC ATGGGGCAAG TGATCGGGAA 
TATCCTCGGC TTGGACAGAT GATCGTTGAC TATGAAAACC CTTTGAAGAA AATGATGGAG 
GAGTTTGTTC CCCATAGTAA GTCTCTATCA GATGCCCTAA TATCACTGCA AATGGTTTAT 
CCACGAAGGA ATCTTTCAGC TGAGCAGTGG CGAAATGCTC AACTTCTAAG TCTCATCAGT 
GCTCCTAGTA CAATGCTAAA CCCAGCACAA TCGGACACGA TGCCATGCGA GTACCTTTCG 
CTTGATGCAA TGGAGAAGTG GATTATTTTT GGATTTATTT TGTGCCATGG AATACTCAAC 
ACTGATGCTA CAGCATTGAA TCTTTGGAAG CTCGCCCTTC AGAGCAGTTC ATGTCTCTGT 
CTTTACAGAG ATGAAGTTTT TCATATCCAC AAGGCAGCAG AAGATCTGTT CGTCAACATT 
AGAGGCTACA ACAAAAGGAT AAATGACATC AGGGAGTGTA AAGAGAATGC AGTATCACAT 
GCAGGAGCAA CACACAGAGA AAGGCGCAAA TTTTTAAGGT CTGCTTTGAA AGAACTTGCT 
ACTGTACTCG CTGATCAGCC AGGCCTTCTG GGTCCCAAAG CACTTTTTGT ATTCATGGCA 
TTATCGTTTG CCCGTGATGA AATAATATGG CTACTTCGTC ATGCAGACAA CATTCCAAAG 
AAGGTCGCAG ATGACTTCAT GGATAAGCAC ATCGCTGAGC TCATATTTTA TATGGAAGAA 
CTGCGAGCGC ATGTACGGAA GTATGGGCCT GTGATGCAGA GATACTATGT GCAGTACCTG 
TCTGGCTTTG ATGCAGTTGT ACTAAATGAG CTAGTGCAGA ATCTTTCTGT GTGCCCTGAG 
GATGAATCAA TTATAATGTC TTCATTCGTA AATACAATGA CCTCCTTGAG TGTAAAACAA 
GTTGAAGACG GAGAGGTTTT TGACTTCAGA GGAATGAGAT TGGATTGGTT CAGATTGCAG 
GCCTATACCA GTGTTTCCAA AGCTTCATTG AGTCTTGCTG ACCACAGAGA ACTTGGAAAA 
ATGATGAACA CTATAATATT CCATACCAAA ATGGTGGATT CCTTAGTGGA GATGTTGGTG 
GAGACATCAG ACCTTTCCAT ATTTTGCTTT TACAGCCGAG CTTTTGAGAA GATGTTTCAG 
CAGTGTTTGG AGCTGCCATC CCAATCAAGA TATTCAATCT CCTTTCCTCT TATATGCACT 
CACTTTATGA GCTGCACTCA CGAACTTTGC CCTGAAGAGC GGCATCACAT TGGAGACCGC 
AGTTTGTCAC TATGTAACAT GTTCTTGGAT GAAATGGCAA AGCAAGCTCG AAATCTAATT 
ACAGACATCT GCACGGAACA GTGTACCCTT AGTGATCAGT TGCTGCCAAA GCACTGTGCC 
AAAACAATCA GTCAAGCGGT GAACAAAAAG TCAAAAAAGC AGACCGGAAA GAAAGGAGAA 
CCAGAAAGGG AAAAACCTGG AGTTGAAAGC ATGAGAAAGA ATAGACTTGT GGTAACAAAC 
CTGGATAAAT TGCACACTGC ACTTTCAGAG CTCTGTTTCT CTATCAACTA TGCACCAAAT 
ATGGTTGTAT GGGAACACAC ATTTACCCCA AGAGAGTATT TGACGTCGCA CTTGGAAATC 
CGCTTTACCA AGTCTATTGT TGGCATGACC ATGTACAATC AAGTCACTCA AGAGATTGCT 
AAACCTTCTG AGTTGTTAAC AAGTGTAAGA GCCTATATGA CTGTACTCCA ATCAATAGAA 
AACTACGTGC AGATTGATAT CACAAGGGTA TTCAACAATG TTCTACTTCA GCAAACACAA 
CATTTAGACA GCCATGGAGA GCCAACAATC ACCAGTTTAT ACACTAATTG GTATTTGGAA 
ACACTACTGA GGCAAGTAAG CAATGGACAT ATAGCATATT TCCCAGCAAT GAAAGCTTTT 
GTAAACCTGC CAACTGAGAA TGAGCTGACG TTTAATGCAG AAGAGTATTC CGACATCTCT 
GAAATGAGGG CATTGTCTGA GCTGCTGGGA CCATATGGCA TGAAGTTTCT CAGTGAAAGC 
CTCATGTGGC ATATTTCCTC TCAGGTGGCT GAGCTGAAGA AACTTGTTGT GGAAAATGTG 
GAGGTCTTAA CCCAGATGAG AACAAGTTTT GATAAACCAG AGCAGATGGC AGCCCTTTTC 
AAAAGGCTTA CATCCGTTGA CAGTGTTTTG AAGAGGATGA CCATTATTGG AGTTATATTG 
TCTTTCCGAT CTCTTGCACA GGAAGCACTA AGAGATGTTT TGTCTTACCA CATTCCTTTT 
CTTGTAAGCT CTGTTGAAGA CTTTAAGGAT CACATTCCTA GAGAGACAGA CATGAAGGTG 
GCTATGAATG TGTATGAGTT ATCATCAGCA GCTGGATTGC CATGTGAAAT AGACCCTGCC 
TTAGTTGTGG CACTGTCATC TCAAAAATCT GAAACCATCA GCCCAGAGGA AGAATACAAA 
ATAGCCTGTC TGCTTATGGT GTTTGTGGCT GTTTCTTTGC CAACATTGGC TAGTAACGTC 
ATGTCGCAAT ACAGCCCTGC AATTGAAGGT CATTGCAACA ACATACACTG CTTGGCAAAG 
GCAATTAACC AAATTGCAGC AGCACTATTT ACCATTCACA AAGGGAGCAT CGAAGATCGT 
CTGAAAGAGT TCTTGGCGCT TGCATCCTCC AGCCTGCTAA AGATTGGCCA AGAGACTGAC 
AAAACTACTA CAAGAAACAG GGAATCTGTT TATTTGCTGC TAGATATGAT TGTTCAAGAA 
TCACCATTCC TGACCATGGA TCTGCTGGAG TCCTGCTTCC CATACGTCTT ACTGAGAAAT 
GCATACCATG CGGTTTACAA ACAGAGTGTC ACATCCTCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap1 ( XM_002932014.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932060.1 Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa16225
Clone ID Related Accession (Same CDS sequence)	XM_002932014.4 , XM_002932014.5
Accession Version	XM_002932014.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932014.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAAGCAA AGCCTTCTTA TCTGATCGAC AAAAATCTTG AATCTGCTGT CAAGTTTATT 
GTCCGGAAAT TTCCAGCAGT TGAAACCCGC AATAATAATC AACAGCTTGC ACAACTTCAA 
AAAGAGAAAT CTGAAATTTT GAAAAACCTG GCACTGTACT ACTTTACCTT TGTTGATGTT 
ATGGAATTTA AGGATCATGT ATGTGAGCTG CTAAACACTA TTGATGTCTG CCAGGTCTTC 
TTTGATATTA CGGTTAACTT TGATTTAACA AAGAACTATC TAGATCTGAT TGTAACCTAC 
ACAACACTAA TGATAATGCT TTCTCGGATT GAGGAGAGAA AAGCAATTAT AGGACTCTAC 
AACTACTCTC ATGAAATGAC CCATGGGGCA AGTGATCGGG AATATCCTCG GCTTGGACAG 
ATGATCGTTG ACTATGAAAA CCCTTTGAAG AAAATGATGG AGGAGTTTGT TCCCCATAGT 
AAGTCTCTAT CAGATGCCCT AATATCACTG CAAATGGTTT ATCCACGAAG GAATCTTTCA 
GCTGAGCAGT GGCGAAATGC TCAACTTCTA AGTCTCATCA GTGCTCCTAG TACAATGCTA 
AACCCAGCAC AATCGGACAC GATGCCATGC GAGTACCTTT CGCTTGATGC AATGGAGAAG 
TGGATTATTT TTGGATTTAT TTTGTGCCAT GGAATACTCA ACACTGATGC TACAGCATTG 
AATCTTTGGA AGCTCGCCCT TCAGAGCAGT TCATGTCTCT GTCTTTACAG AGATGAAGTT 
TTTCATATCC ACAAGGCAGC AGAAGATCTG TTCGTCAACA TTAGAGGCTA CAACAAAAGG 
ATAAATGACA TCAGGGAGTG TAAAGAGAAT GCAGTATCAC ATGCAGGAGC AACACACAGA 
GAAAGGCGCA AATTTTTAAG GTCTGCTTTG AAAGAACTTG CTACTGTACT CGCTGATCAG 
CCAGGCCTTC TGGGTCCCAA AGCACTTTTT GTATTCATGG CATTATCGTT TGCCCGTGAT 
GAAATAATAT GGCTACTTCG TCATGCAGAC AACATTCCAA AGAAGGTCGC AGATGACTTC 
ATGGATAAGC ACATCGCTGA GCTCATATTT TATATGGAAG AACTGCGAGC GCATGTACGG 
AAGTATGGGC CTGTGATGCA GAGATACTAT GTGCAGTACC TGTCTGGCTT TGATGCAGTT 
GTACTAAATG AGCTAGTGCA GAATCTTTCT GTGTGCCCTG AGGATGAATC AATTATAATG 
TCTTCATTCG TAAATACAAT GACCTCCTTG AGTGTAAAAC AAGTTGAAGA CGGAGAGGTT 
TTTGACTTCA GAGGAATGAG ATTGGATTGG TTCAGATTGC AGGCCTATAC CAGTGTTTCC 
AAAGCTTCAT TGAGTCTTGC TGACCACAGA GAACTTGGAA AAATGATGAA CACTATAATA 
TTCCATACCA AAATGGTGGA TTCCTTAGTG GAGATGTTGG TGGAGACATC AGACCTTTCC 
ATATTTTGCT TTTACAGCCG AGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTGCCA 
TCCCAATCAA GATATTCAAT CTCCTTTCCT CTTATATGCA CTCACTTTAT GAGCTGCACT 
CACGAACTTT GCCCTGAAGA GCGGCATCAC ATTGGAGACC GCAGTTTGTC ACTATGTAAC 
ATGTTCTTGG ATGAAATGGC AAAGCAAGCT CGAAATCTAA TTACAGACAT CTGCACGGAA 
CAGTGTACCC TTAGTGATCA GTTGCTGCCA AAGCACTGTG CCAAAACAAT CAGTCAAGCG 
GTGAACAAAA AGTCAAAAAA GCAGACCGGA AAGAAAGGAG AACCAGAAAG GGAAAAACCT 
GGAGTTGAAA GCATGAGAAA GAATAGACTT GTGGTAACAA ACCTGGATAA ATTGCACACT 
GCACTTTCAG AGCTCTGTTT CTCTATCAAC TATGCACCAA ATATGGTTGT ATGGGAACAC 
ACATTTACCC CAAGAGAGTA TTTGACGTCG CACTTGGAAA TCCGCTTTAC CAAGTCTATT 
GTTGGCATGA CCATGTACAA TCAAGTCACT CAAGAGATTG CTAAACCTTC TGAGTTGTTA 
ACAAGTGTAA GAGCCTATAT GACTGTACTC CAATCAATAG AAAACTACGT GCAGATTGAT 
ATCACAAGGG TATTCAACAA TGTTCTACTT CAGCAAACAC AACATTTAGA CAGCCATGGA 
GAGCCAACAA TCACCAGTTT ATACACTAAT TGGTATTTGG AAACACTACT GAGGCAAGTA 
AGCAATGGAC ATATAGCATA TTTCCCAGCA ATGAAAGCTT TTGTAAACCT GCCAACTGAG 
AATGAGCTGA CGTTTAATGC AGAAGAGTAT TCCGACATCT CTGAAATGAG GGCATTGTCT 
GAGCTGCTGG GACCATATGG CATGAAGTTT CTCAGTGAAA GCCTCATGTG GCATATTTCC 
TCTCAGGTGG CTGAGCTGAA GAAACTTGTT GTGGAAAATG TGGAGGTCTT AACCCAGATG 
AGAACAAGTT TTGATAAACC AGAGCAGATG GCAGCCCTTT TCAAAAGGCT TACATCCGTT 
GACAGTGTTT TGAAGAGGAT GACCATTATT GGAGTTATAT TGTCTTTCCG ATCTCTTGCA 
CAGGAAGCAC TAAGAGATGT TTTGTCTTAC CACATTCCTT TTCTTGTAAG CTCTGTTGAA 
GACTTTAAGG ATCACATTCC TAGAGAGACA GACATGAAGG TGGCTATGAA TGTGTATGAG 
TTATCATCAG CAGCTGGATT GCCATGTGAA ATAGACCCTG CCTTAGTTGT GGCACTGTCA 
TCTCAAAAAT CTGAAACCAT CAGCCCAGAG GAAGAATACA AAATAGCCTG TCTGCTTATG 
GTGTTTGTGG CTGTTTCTTT GCCAACATTG GCTAGTAACG TCATGTCGCA ATACAGCCCT 
GCAATTGAAG GTCATTGCAA CAACATACAC TGCTTGGCAA AGGCAATTAA CCAAATTGCA 
GCAGCACTAT TTACCATTCA CAAAGGGAGC ATCGAAGATC GTCTGAAAGA GTTCTTGGCG 
CTTGCATCCT CCAGCCTGCT AAAGATTGGC CAAGAGACTG ACAAAACTAC TACAAGAAAC 
AGGGAATCTG TTTATTTGCT GCTAGATATG ATTGTTCAAG AATCACCATT CCTGACCATG 
GATCTGCTGG AGTCCTGCTT CCCATACGTC TTACTGAGAA ATGCATACCA TGCGGTTTAC 
AAACAGAGTG TCACATCCTC TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932060.1
CDS	282..3668
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIVTYTTLMIMLSRIEERKAIIGLYNYSHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSAEQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLCLYRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKENAVSHAGATHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIPKKVADDFMDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLICTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYAPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQVTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPEQMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSETISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_002932014.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932014.5

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAAGCAA AGCCTTCTTA TCTGATCGAC AAAAATCTTG AATCTGCTGT CAAGTTTATT 
GTCCGGAAAT TTCCAGCAGT TGAAACCCGC AATAATAATC AACAGCTTGC ACAACTTCAA 
AAAGAGAAAT CTGAAATTTT GAAAAACCTG GCACTGTACT ACTTTACCTT TGTTGATGTT 
ATGGAATTTA AGGATCATGT ATGTGAGCTG CTAAACACTA TTGATGTCTG CCAGGTCTTC 
TTTGATATTA CGGTTAACTT TGATTTAACA AAGAACTATC TAGATCTGAT TGTAACCTAC 
ACAACACTAA TGATAATGCT TTCTCGGATT GAGGAGAGAA AAGCAATTAT AGGACTCTAC 
AACTACTCTC ATGAAATGAC CCATGGGGCA AGTGATCGGG AATATCCTCG GCTTGGACAG 
ATGATCGTTG ACTATGAAAA CCCTTTGAAG AAAATGATGG AGGAGTTTGT TCCCCATAGT 
AAGTCTCTAT CAGATGCCCT AATATCACTG CAAATGGTTT ATCCACGAAG GAATCTTTCA 
GCTGAGCAGT GGCGAAATGC TCAACTTCTA AGTCTCATCA GTGCTCCTAG TACAATGCTA 
AACCCAGCAC AATCGGACAC GATGCCATGC GAGTACCTTT CGCTTGATGC AATGGAGAAG 
TGGATTATTT TTGGATTTAT TTTGTGCCAT GGAATACTCA ACACTGATGC TACAGCATTG 
AATCTTTGGA AGCTCGCCCT TCAGAGCAGT TCATGTCTCT GTCTTTACAG AGATGAAGTT 
TTTCATATCC ACAAGGCAGC AGAAGATCTG TTCGTCAACA TTAGAGGCTA CAACAAAAGG 
ATAAATGACA TCAGGGAGTG TAAAGAGAAT GCAGTATCAC ATGCAGGAGC AACACACAGA 
GAAAGGCGCA AATTTTTAAG GTCTGCTTTG AAAGAACTTG CTACTGTACT CGCTGATCAG 
CCAGGCCTTC TGGGTCCCAA AGCACTTTTT GTATTCATGG CATTATCGTT TGCCCGTGAT 
GAAATAATAT GGCTACTTCG TCATGCAGAC AACATTCCAA AGAAGGTCGC AGATGACTTC 
ATGGATAAGC ACATCGCTGA GCTCATATTT TATATGGAAG AACTGCGAGC GCATGTACGG 
AAGTATGGGC CTGTGATGCA GAGATACTAT GTGCAGTACC TGTCTGGCTT TGATGCAGTT 
GTACTAAATG AGCTAGTGCA GAATCTTTCT GTGTGCCCTG AGGATGAATC AATTATAATG 
TCTTCATTCG TAAATACAAT GACCTCCTTG AGTGTAAAAC AAGTTGAAGA CGGAGAGGTT 
TTTGACTTCA GAGGAATGAG ATTGGATTGG TTCAGATTGC AGGCCTATAC CAGTGTTTCC 
AAAGCTTCAT TGAGTCTTGC TGACCACAGA GAACTTGGAA AAATGATGAA CACTATAATA 
TTCCATACCA AAATGGTGGA TTCCTTAGTG GAGATGTTGG TGGAGACATC AGACCTTTCC 
ATATTTTGCT TTTACAGCCG AGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTGCCA 
TCCCAATCAA GATATTCAAT CTCCTTTCCT CTTATATGCA CTCACTTTAT GAGCTGCACT 
CACGAACTTT GCCCTGAAGA GCGGCATCAC ATTGGAGACC GCAGTTTGTC ACTATGTAAC 
ATGTTCTTGG ATGAAATGGC AAAGCAAGCT CGAAATCTAA TTACAGACAT CTGCACGGAA 
CAGTGTACCC TTAGTGATCA GTTGCTGCCA AAGCACTGTG CCAAAACAAT CAGTCAAGCG 
GTGAACAAAA AGTCAAAAAA GCAGACCGGA AAGAAAGGAG AACCAGAAAG GGAAAAACCT 
GGAGTTGAAA GCATGAGAAA GAATAGACTT GTGGTAACAA ACCTGGATAA ATTGCACACT 
GCACTTTCAG AGCTCTGTTT CTCTATCAAC TATGCACCAA ATATGGTTGT ATGGGAACAC 
ACATTTACCC CAAGAGAGTA TTTGACGTCG CACTTGGAAA TCCGCTTTAC CAAGTCTATT 
GTTGGCATGA CCATGTACAA TCAAGTCACT CAAGAGATTG CTAAACCTTC TGAGTTGTTA 
ACAAGTGTAA GAGCCTATAT GACTGTACTC CAATCAATAG AAAACTACGT GCAGATTGAT 
ATCACAAGGG TATTCAACAA TGTTCTACTT CAGCAAACAC AACATTTAGA CAGCCATGGA 
GAGCCAACAA TCACCAGTTT ATACACTAAT TGGTATTTGG AAACACTACT GAGGCAAGTA 
AGCAATGGAC ATATAGCATA TTTCCCAGCA ATGAAAGCTT TTGTAAACCT GCCAACTGAG 
AATGAGCTGA CGTTTAATGC AGAAGAGTAT TCCGACATCT CTGAAATGAG GGCATTGTCT 
GAGCTGCTGG GACCATATGG CATGAAGTTT CTCAGTGAAA GCCTCATGTG GCATATTTCC 
TCTCAGGTGG CTGAGCTGAA GAAACTTGTT GTGGAAAATG TGGAGGTCTT AACCCAGATG 
AGAACAAGTT TTGATAAACC AGAGCAGATG GCAGCCCTTT TCAAAAGGCT TACATCCGTT 
GACAGTGTTT TGAAGAGGAT GACCATTATT GGAGTTATAT TGTCTTTCCG ATCTCTTGCA 
CAGGAAGCAC TAAGAGATGT TTTGTCTTAC CACATTCCTT TTCTTGTAAG CTCTGTTGAA 
GACTTTAAGG ATCACATTCC TAGAGAGACA GACATGAAGG TGGCTATGAA TGTGTATGAG 
TTATCATCAG CAGCTGGATT GCCATGTGAA ATAGACCCTG CCTTAGTTGT GGCACTGTCA 
TCTCAAAAAT CTGAAACCAT CAGCCCAGAG GAAGAATACA AAATAGCCTG TCTGCTTATG 
GTGTTTGTGG CTGTTTCTTT GCCAACATTG GCTAGTAACG TCATGTCGCA ATACAGCCCT 
GCAATTGAAG GTCATTGCAA CAACATACAC TGCTTGGCAA AGGCAATTAA CCAAATTGCA 
GCAGCACTAT TTACCATTCA CAAAGGGAGC ATCGAAGATC GTCTGAAAGA GTTCTTGGCG 
CTTGCATCCT CCAGCCTGCT AAAGATTGGC CAAGAGACTG ACAAAACTAC TACAAGAAAC 
AGGGAATCTG TTTATTTGCT GCTAGATATG ATTGTTCAAG AATCACCATT CCTGACCATG 
GATCTGCTGG AGTCCTGCTT CCCATACGTC TTACTGAGAA ATGCATACCA TGCGGTTTAC 
AAACAGAGTG TCACATCCTC TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap1 ( XM_004917911.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917968.1 Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X5, mRNA.

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CloneID	OXa16226
Clone ID Related Accession (Same CDS sequence)	XM_004917911.3 , XM_004917911.4
Accession Version	XM_004917911.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004917911.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAAGCAA AGCCTTCTTA TCTGATCGAC AAAAATCTTG AATCTGCTGT CAAGTTTATT 
GTCCGGAAAT TTCCAGCAGT TGAAACCCGC AATAATAATC TTGCACAACT TCAAAAAGAG 
AAATCTGAAA TTTTGAAAAA CCTGGCACTG TACTACTTTA CCTTTGTTGA TGTTATGGAA 
TTTAAGGATC ATGTATGTGA GCTGCTAAAC ACTATTGATG TCTGCCAGGT CTTCTTTGAT 
ATTACGGTTA ACTTTGATTT AACAAAGAAC TATCTAGATC TGATTGTAAC CTACACAACA 
CTAATGATAA TGCTTTCTCG GATTGAGGAG AGAAAAGCAA TTATAGGACT CTACAACTAC 
TCTCATGAAA TGACCCATGG GGCAAGTGAT CGGGAATATC CTCGGCTTGG ACAGATGATC 
GTTGACTATG AAAACCCTTT GAAGAAAATG ATGGAGGAGT TTGTTCCCCA TAGTAAGTCT 
CTATCAGATG CCCTAATATC ACTGCAAATG GTTTATCCAC GAAGGAATCT TTCAGCTGAG 
CAGTGGCGAA ATGCTCAACT TCTAAGTCTC ATCAGTGCTC CTAGTACAAT GCTAAACCCA 
GCACAATCGG ACACGATGCC ATGCGAGTAC CTTTCGCTTG ATGCAATGGA GAAGTGGATT 
ATTTTTGGAT TTATTTTGTG CCATGGAATA CTCAACACTG ATGCTACAGC ATTGAATCTT 
TGGAAGCTCG CCCTTCAGAG CAGTTCATGT CTCTGTCTTT ACAGAGATGA AGTTTTTCAT 
ATCCACAAGG CAGCAGAAGA TCTGTTCGTC AACATTAGAG GCTACAACAA AAGGATAAAT 
GACATCAGGG AGTGTAAAGA GAATGCAGTA TCACATGCAG GAGCAACACA CAGAGAAAGG 
CGCAAATTTT TAAGGTCTGC TTTGAAAGAA CTTGCTACTG TACTCGCTGA TCAGCCAGGC 
CTTCTGGGTC CCAAAGCACT TTTTGTATTC ATGGCATTAT CGTTTGCCCG TGATGAAATA 
ATATGGCTAC TTCGTCATGC AGACAACATT CCAAAGAAGG TCGCAGATGA CTTCATGGAT 
AAGCACATCG CTGAGCTCAT ATTTTATATG GAAGAACTGC GAGCGCATGT ACGGAAGTAT 
GGGCCTGTGA TGCAGAGATA CTATGTGCAG TACCTGTCTG GCTTTGATGC AGTTGTACTA 
AATGAGCTAG TGCAGAATCT TTCTGTGTGC CCTGAGGATG AATCAATTAT AATGTCTTCA 
TTCGTAAATA CAATGACCTC CTTGAGTGTA AAACAAGTTG AAGACGGAGA GGTTTTTGAC 
TTCAGAGGAA TGAGATTGGA TTGGTTCAGA TTGCAGGCCT ATACCAGTGT TTCCAAAGCT 
TCATTGAGTC TTGCTGACCA CAGAGAACTT GGAAAAATGA TGAACACTAT AATATTCCAT 
ACCAAAATGG TGGATTCCTT AGTGGAGATG TTGGTGGAGA CATCAGACCT TTCCATATTT 
TGCTTTTACA GCCGAGCTTT TGAGAAGATG TTTCAGCAGT GTTTGGAGCT GCCATCCCAA 
TCAAGATATT CAATCTCCTT TCCTCTTATA TGCACTCACT TTATGAGCTG CACTCACGAA 
CTTTGCCCTG AAGAGCGGCA TCACATTGGA GACCGCAGTT TGTCACTATG TAACATGTTC 
TTGGATGAAA TGGCAAAGCA AGCTCGAAAT CTAATTACAG ACATCTGCAC GGAACAGTGT 
ACCCTTAGTG ATCAGTTGCT GCCAAAGCAC TGTGCCAAAA CAATCAGTCA AGCGGTGAAC 
AAAAAGTCAA AAAAGCAGAC CGGAAAGAAA GGAGAACCAG AAAGGGAAAA ACCTGGAGTT 
GAAAGCATGA GAAAGAATAG ACTTGTGGTA ACAAACCTGG ATAAATTGCA CACTGCACTT 
TCAGAGCTCT GTTTCTCTAT CAACTATGCA CCAAATATGG TTGTATGGGA ACACACATTT 
ACCCCAAGAG AGTATTTGAC GTCGCACTTG GAAATCCGCT TTACCAAGTC TATTGTTGGC 
ATGACCATGT ACAATCAAGT CACTCAAGAG ATTGCTAAAC CTTCTGAGTT GTTAACAAGT 
GTAAGAGCCT ATATGACTGT ACTCCAATCA ATAGAAAACT ACGTGCAGAT TGATATCACA 
AGGGTATTCA ACAATGTTCT ACTTCAGCAA ACACAACATT TAGACAGCCA TGGAGAGCCA 
ACAATCACCA GTTTATACAC TAATTGGTAT TTGGAAACAC TACTGAGGCA AGTAAGCAAT 
GGACATATAG CATATTTCCC AGCAATGAAA GCTTTTGTAA ACCTGCCAAC TGAGAATGAG 
CTGACGTTTA ATGCAGAAGA GTATTCCGAC ATCTCTGAAA TGAGGGCATT GTCTGAGCTG 
CTGGGACCAT ATGGCATGAA GTTTCTCAGT GAAAGCCTCA TGTGGCATAT TTCCTCTCAG 
GTGGCTGAGC TGAAGAAACT TGTTGTGGAA AATGTGGAGG TCTTAACCCA GATGAGAACA 
AGTTTTGATA AACCAGAGCA GATGGCAGCC CTTTTCAAAA GGCTTACATC CGTTGACAGT 
GTTTTGAAGA GGATGACCAT TATTGGAGTT ATATTGTCTT TCCGATCTCT TGCACAGGAA 
GCACTAAGAG ATGTTTTGTC TTACCACATT CCTTTTCTTG TAAGCTCTGT TGAAGACTTT 
AAGGATCACA TTCCTAGAGA GACAGACATG AAGGTGGCTA TGAATGTGTA TGAGTTATCA 
TCAGCAGCTG GATTGCCATG TGAAATAGAC CCTGCCTTAG TTGTGGCACT GTCATCTCAA 
AAATCTGAAA CCATCAGCCC AGAGGAAGAA TACAAAATAG CCTGTCTGCT TATGGTGTTT 
GTGGCTGTTT CTTTGCCAAC ATTGGCTAGT AACGTCATGT CGCAATACAG CCCTGCAATT 
GAAGGTCATT GCAACAACAT ACACTGCTTG GCAAAGGCAA TTAACCAAAT TGCAGCAGCA 
CTATTTACCA TTCACAAAGG GAGCATCGAA GATCGTCTGA AAGAGTTCTT GGCGCTTGCA 
TCCTCCAGCC TGCTAAAGAT TGGCCAAGAG ACTGACAAAA CTACTACAAG AAACAGGGAA 
TCTGTTTATT TGCTGCTAGA TATGATTGTT CAAGAATCAC CATTCCTGAC CATGGATCTG 
CTGGAGTCCT GCTTCCCATA CGTCTTACTG AGAAATGCAT ACCATGCGGT TTACAAACAG 
AGTGTCACAT CCTCTGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917968.1
CDS	168..3548
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIVTYTTLMIMLSRIEERKAIIGLYNYSHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSAEQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLCLYRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKENAVSHAGATHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIPKKVADDFMDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLICTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYAPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQVTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPEQMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSETISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_004917911.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917911.4

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAAGCAA AGCCTTCTTA TCTGATCGAC AAAAATCTTG AATCTGCTGT CAAGTTTATT 
GTCCGGAAAT TTCCAGCAGT TGAAACCCGC AATAATAATC TTGCACAACT TCAAAAAGAG 
AAATCTGAAA TTTTGAAAAA CCTGGCACTG TACTACTTTA CCTTTGTTGA TGTTATGGAA 
TTTAAGGATC ATGTATGTGA GCTGCTAAAC ACTATTGATG TCTGCCAGGT CTTCTTTGAT 
ATTACGGTTA ACTTTGATTT AACAAAGAAC TATCTAGATC TGATTGTAAC CTACACAACA 
CTAATGATAA TGCTTTCTCG GATTGAGGAG AGAAAAGCAA TTATAGGACT CTACAACTAC 
TCTCATGAAA TGACCCATGG GGCAAGTGAT CGGGAATATC CTCGGCTTGG ACAGATGATC 
GTTGACTATG AAAACCCTTT GAAGAAAATG ATGGAGGAGT TTGTTCCCCA TAGTAAGTCT 
CTATCAGATG CCCTAATATC ACTGCAAATG GTTTATCCAC GAAGGAATCT TTCAGCTGAG 
CAGTGGCGAA ATGCTCAACT TCTAAGTCTC ATCAGTGCTC CTAGTACAAT GCTAAACCCA 
GCACAATCGG ACACGATGCC ATGCGAGTAC CTTTCGCTTG ATGCAATGGA GAAGTGGATT 
ATTTTTGGAT TTATTTTGTG CCATGGAATA CTCAACACTG ATGCTACAGC ATTGAATCTT 
TGGAAGCTCG CCCTTCAGAG CAGTTCATGT CTCTGTCTTT ACAGAGATGA AGTTTTTCAT 
ATCCACAAGG CAGCAGAAGA TCTGTTCGTC AACATTAGAG GCTACAACAA AAGGATAAAT 
GACATCAGGG AGTGTAAAGA GAATGCAGTA TCACATGCAG GAGCAACACA CAGAGAAAGG 
CGCAAATTTT TAAGGTCTGC TTTGAAAGAA CTTGCTACTG TACTCGCTGA TCAGCCAGGC 
CTTCTGGGTC CCAAAGCACT TTTTGTATTC ATGGCATTAT CGTTTGCCCG TGATGAAATA 
ATATGGCTAC TTCGTCATGC AGACAACATT CCAAAGAAGG TCGCAGATGA CTTCATGGAT 
AAGCACATCG CTGAGCTCAT ATTTTATATG GAAGAACTGC GAGCGCATGT ACGGAAGTAT 
GGGCCTGTGA TGCAGAGATA CTATGTGCAG TACCTGTCTG GCTTTGATGC AGTTGTACTA 
AATGAGCTAG TGCAGAATCT TTCTGTGTGC CCTGAGGATG AATCAATTAT AATGTCTTCA 
TTCGTAAATA CAATGACCTC CTTGAGTGTA AAACAAGTTG AAGACGGAGA GGTTTTTGAC 
TTCAGAGGAA TGAGATTGGA TTGGTTCAGA TTGCAGGCCT ATACCAGTGT TTCCAAAGCT 
TCATTGAGTC TTGCTGACCA CAGAGAACTT GGAAAAATGA TGAACACTAT AATATTCCAT 
ACCAAAATGG TGGATTCCTT AGTGGAGATG TTGGTGGAGA CATCAGACCT TTCCATATTT 
TGCTTTTACA GCCGAGCTTT TGAGAAGATG TTTCAGCAGT GTTTGGAGCT GCCATCCCAA 
TCAAGATATT CAATCTCCTT TCCTCTTATA TGCACTCACT TTATGAGCTG CACTCACGAA 
CTTTGCCCTG AAGAGCGGCA TCACATTGGA GACCGCAGTT TGTCACTATG TAACATGTTC 
TTGGATGAAA TGGCAAAGCA AGCTCGAAAT CTAATTACAG ACATCTGCAC GGAACAGTGT 
ACCCTTAGTG ATCAGTTGCT GCCAAAGCAC TGTGCCAAAA CAATCAGTCA AGCGGTGAAC 
AAAAAGTCAA AAAAGCAGAC CGGAAAGAAA GGAGAACCAG AAAGGGAAAA ACCTGGAGTT 
GAAAGCATGA GAAAGAATAG ACTTGTGGTA ACAAACCTGG ATAAATTGCA CACTGCACTT 
TCAGAGCTCT GTTTCTCTAT CAACTATGCA CCAAATATGG TTGTATGGGA ACACACATTT 
ACCCCAAGAG AGTATTTGAC GTCGCACTTG GAAATCCGCT TTACCAAGTC TATTGTTGGC 
ATGACCATGT ACAATCAAGT CACTCAAGAG ATTGCTAAAC CTTCTGAGTT GTTAACAAGT 
GTAAGAGCCT ATATGACTGT ACTCCAATCA ATAGAAAACT ACGTGCAGAT TGATATCACA 
AGGGTATTCA ACAATGTTCT ACTTCAGCAA ACACAACATT TAGACAGCCA TGGAGAGCCA 
ACAATCACCA GTTTATACAC TAATTGGTAT TTGGAAACAC TACTGAGGCA AGTAAGCAAT 
GGACATATAG CATATTTCCC AGCAATGAAA GCTTTTGTAA ACCTGCCAAC TGAGAATGAG 
CTGACGTTTA ATGCAGAAGA GTATTCCGAC ATCTCTGAAA TGAGGGCATT GTCTGAGCTG 
CTGGGACCAT ATGGCATGAA GTTTCTCAGT GAAAGCCTCA TGTGGCATAT TTCCTCTCAG 
GTGGCTGAGC TGAAGAAACT TGTTGTGGAA AATGTGGAGG TCTTAACCCA GATGAGAACA 
AGTTTTGATA AACCAGAGCA GATGGCAGCC CTTTTCAAAA GGCTTACATC CGTTGACAGT 
GTTTTGAAGA GGATGACCAT TATTGGAGTT ATATTGTCTT TCCGATCTCT TGCACAGGAA 
GCACTAAGAG ATGTTTTGTC TTACCACATT CCTTTTCTTG TAAGCTCTGT TGAAGACTTT 
AAGGATCACA TTCCTAGAGA GACAGACATG AAGGTGGCTA TGAATGTGTA TGAGTTATCA 
TCAGCAGCTG GATTGCCATG TGAAATAGAC CCTGCCTTAG TTGTGGCACT GTCATCTCAA 
AAATCTGAAA CCATCAGCCC AGAGGAAGAA TACAAAATAG CCTGTCTGCT TATGGTGTTT 
GTGGCTGTTT CTTTGCCAAC ATTGGCTAGT AACGTCATGT CGCAATACAG CCCTGCAATT 
GAAGGTCATT GCAACAACAT ACACTGCTTG GCAAAGGCAA TTAACCAAAT TGCAGCAGCA 
CTATTTACCA TTCACAAAGG GAGCATCGAA GATCGTCTGA AAGAGTTCTT GGCGCTTGCA 
TCCTCCAGCC TGCTAAAGAT TGGCCAAGAG ACTGACAAAA CTACTACAAG AAACAGGGAA 
TCTGTTTATT TGCTGCTAGA TATGATTGTT CAAGAATCAC CATTCCTGAC CATGGATCTG 
CTGGAGTCCT GCTTCCCATA CGTCTTACTG AGAAATGCAT ACCATGCGGT TTACAAACAG 
AGTGTCACAT CCTCTGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap1 ( XM_004917909.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917966.1 Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa16222
Clone ID Related Accession (Same CDS sequence)	XM_004917909.3 , XM_004917909.4
Accession Version	XM_004917909.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917909.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGCA AGGACAAGTC 
TGGAAGGCAT GTGGAGACCC CAAAGCAAAG CCTTCTTATC TGATCGACAA AAATCTTGAA 
TCTGCTGTCA AGTTTATTGT CCGGAAATTT CCAGCAGTTG AAACCCGCAA TAATAATCAA 
CAGCTTGCAC AACTTCAAAA AGAGAAATCT GAAATTTTGA AAAACCTGGC ACTGTACTAC 
TTTACCTTTG TTGATGTTAT GGAATTTAAG GATCATGTAT GTGAGCTGCT AAACACTATT 
GATGTCTGCC AGGTCTTCTT TGATATTACG GTTAACTTTG ATTTAACAAA GAACTATCTA 
GATCTGATTG TAACCTACAC AACACTAATG ATAATGCTTT CTCGGATTGA GGAGAGAAAA 
GCAATTATAG GACTCTACAA CTACTCTCAT GAAATGACCC ATGGGGCAAG TGATCGGGAA 
TATCCTCGGC TTGGACAGAT GATCGTTGAC TATGAAAACC CTTTGAAGAA AATGATGGAG 
GAGTTTGTTC CCCATAGTAA GTCTCTATCA GATGCCCTAA TATCACTGCA AATGGTTTAT 
CCACGAAGGA ATCTTTCAGC TGAGCAGTGG CGAAATGCTC AACTTCTAAG TCTCATCAGT 
GCTCCTAGTA CAATGCTAAA CCCAGCACAA TCGGACACGA TGCCATGCGA GTACCTTTCG 
CTTGATGCAA TGGAGAAGTG GATTATTTTT GGATTTATTT TGTGCCATGG AATACTCAAC 
ACTGATGCTA CAGCATTGAA TCTTTGGAAG CTCGCCCTTC AGAGCAGTTC ATGTCTCTGT 
CTTTACAGAG ATGAAGTTTT TCATATCCAC AAGGCAGCAG AAGATCTGTT CGTCAACATT 
AGAGGCTACA ACAAAAGGAT AAATGACATC AGGGAGTGTA AAGAGAATGC AGTATCACAT 
GCAGGAGCAA CACACAGAGA AAGGCGCAAA TTTTTAAGGT CTGCTTTGAA AGAACTTGCT 
ACTGTACTCG CTGATCAGCC AGGCCTTCTG GGTCCCAAAG CACTTTTTGT ATTCATGGCA 
TTATCGTTTG CCCGTGATGA AATAATATGG CTACTTCGTC ATGCAGACAA CATTCCAAAG 
AAGGTCGCAG ATGACTTCAT GGATAAGCAC ATCGCTGAGC TCATATTTTA TATGGAAGAA 
CTGCGAGCGC ATGTACGGAA GTATGGGCCT GTGATGCAGA GATACTATGT GCAGTACCTG 
TCTGGCTTTG ATGCAGTTGT ACTAAATGAG CTAGTGCAGA ATCTTTCTGT GTGCCCTGAG 
GATGAATCAA TTATAATGTC TTCATTCGTA AATACAATGA CCTCCTTGAG TGTAAAACAA 
GTTGAAGACG GAGAGGTTTT TGACTTCAGA GGAATGAGAT TGGATTGGTT CAGATTGCAG 
GCCTATACCA GTGTTTCCAA AGCTTCATTG AGTCTTGCTG ACCACAGAGA ACTTGGAAAA 
ATGATGAACA CTATAATATT CCATACCAAA ATGGTGGATT CCTTAGTGGA GATGTTGGTG 
GAGACATCAG ACCTTTCCAT ATTTTGCTTT TACAGCCGAG CTTTTGAGAA GATGTTTCAG 
CAGTGTTTGG AGCTGCCATC CCAATCAAGA TATTCAATCT CCTTTCCTCT TATATGCACT 
CACTTTATGA GCTGCACTCA CGAACTTTGC CCTGAAGAGG TAATTTTTAT TAATTCCCAG 
CGGCATCACA TTGGAGACCG CAGTTTGTCA CTATGTAACA TGTTCTTGGA TGAAATGGCA 
AAGCAAGCTC GAAATCTAAT TACAGACATC TGCACGGAAC AGTGTACCCT TAGTGATCAG 
TTGCTGCCAA AGCACTGTGC CAAAACAATC AGTCAAGCGG TGAACAAAAA GTCAAAAAAG 
CAGACCGGAA AGAAAGGAGA ACCAGAAAGG GAAAAACCTG GAGTTGAAAG CATGAGAAAG 
AATAGACTTG TGGTAACAAA CCTGGATAAA TTGCACACTG CACTTTCAGA GCTCTGTTTC 
TCTATCAACT ATGCACCAAA TATGGTTGTA TGGGAACACA CATTTACCCC AAGAGAGTAT 
TTGACGTCGC ACTTGGAAAT CCGCTTTACC AAGTCTATTG TTGGCATGAC CATGTACAAT 
CAAGTCACTC AAGAGATTGC TAAACCTTCT GAGTTGTTAA CAAGTGTAAG AGCCTATATG 
ACTGTACTCC AATCAATAGA AAACTACGTG CAGATTGATA TCACAAGGGT ATTCAACAAT 
GTTCTACTTC AGCAAACACA ACATTTAGAC AGCCATGGAG AGCCAACAAT CACCAGTTTA 
TACACTAATT GGTATTTGGA AACACTACTG AGGCAAGTAA GCAATGGACA TATAGCATAT 
TTCCCAGCAA TGAAAGCTTT TGTAAACCTG CCAACTGAGA ATGAGCTGAC GTTTAATGCA 
GAAGAGTATT CCGACATCTC TGAAATGAGG GCATTGTCTG AGCTGCTGGG ACCATATGGC 
ATGAAGTTTC TCAGTGAAAG CCTCATGTGG CATATTTCCT CTCAGGTGGC TGAGCTGAAG 
AAACTTGTTG TGGAAAATGT GGAGGTCTTA ACCCAGATGA GAACAAGTTT TGATAAACCA 
GAGCAGATGG CAGCCCTTTT CAAAAGGCTT ACATCCGTTG ACAGTGTTTT GAAGAGGATG 
ACCATTATTG GAGTTATATT GTCTTTCCGA TCTCTTGCAC AGGAAGCACT AAGAGATGTT 
TTGTCTTACC ACATTCCTTT TCTTGTAAGC TCTGTTGAAG ACTTTAAGGA TCACATTCCT 
AGAGAGACAG ACATGAAGGT GGCTATGAAT GTGTATGAGT TATCATCAGC AGCTGGATTG 
CCATGTGAAA TAGACCCTGC CTTAGTTGTG GCACTGTCAT CTCAAAAATC TGAAACCATC 
AGCCCAGAGG AAGAATACAA AATAGCCTGT CTGCTTATGG TGTTTGTGGC TGTTTCTTTG 
CCAACATTGG CTAGTAACGT CATGTCGCAA TACAGCCCTG CAATTGAAGG TCATTGCAAC 
AACATACACT GCTTGGCAAA GGCAATTAAC CAAATTGCAG CAGCACTATT TACCATTCAC 
AAAGGGAGCA TCGAAGATCG TCTGAAAGAG TTCTTGGCGC TTGCATCCTC CAGCCTGCTA 
AAGATTGGCC AAGAGACTGA CAAAACTACT ACAAGAAACA GGGAATCTGT TTATTTGCTG 
CTAGATATGA TTGTTCAAGA ATCACCATTC CTGACCATGG ATCTGCTGGA GTCCTGCTTC 
CCATACGTCT TACTGAGAAA TGCATACCAT GCGGTTTACA AACAGAGTGT CACATCCTCT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917966.1
CDS	295..3720
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIVTYTTLMIMLSRIEERKAIIGLYNYSHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSAEQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLCLYRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKENAVSHAGATHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIPKKVADDFMDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLICTHFMSCTHELCPEEVIFINSQRHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYAPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQVTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPEQMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSETISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_004917909.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917909.3

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGCA AGGACAAGTC 
TGGAAGGCAT GTGGAGACCC CAAAGCAAAG CCTTCTTATC TGATCGACAA AAATCTTGAA 
TCTGCTGTCA AGTTTATTGT CCGGAAATTT CCAGCAGTTG AAACCCGCAA TAATAATCAA 
CAGCTTGCAC AACTTCAAAA AGAGAAATCT GAAATTTTGA AAAACCTGGC ACTGTACTAC 
TTTACCTTTG TTGATGTTAT GGAATTTAAG GATCATGTAT GTGAGCTGCT AAACACTATT 
GATGTCTGCC AGGTCTTCTT TGATATTACG GTTAACTTTG ATTTAACAAA GAACTATCTA 
GATCTGATTG TAACCTACAC AACACTAATG ATAATGCTTT CTCGGATTGA GGAGAGAAAA 
GCAATTATAG GACTCTACAA CTACTCTCAT GAAATGACCC ATGGGGCAAG TGATCGGGAA 
TATCCTCGGC TTGGACAGAT GATCGTTGAC TATGAAAACC CTTTGAAGAA AATGATGGAG 
GAGTTTGTTC CCCATAGTAA GTCTCTATCA GATGCCCTAA TATCACTGCA AATGGTTTAT 
CCACGAAGGA ATCTTTCAGC TGAGCAGTGG CGAAATGCTC AACTTCTAAG TCTCATCAGT 
GCTCCTAGTA CAATGCTAAA CCCAGCACAA TCGGACACGA TGCCATGCGA GTACCTTTCG 
CTTGATGCAA TGGAGAAGTG GATTATTTTT GGATTTATTT TGTGCCATGG AATACTCAAC 
ACTGATGCTA CAGCATTGAA TCTTTGGAAG CTCGCCCTTC AGAGCAGTTC ATGTCTCTGT 
CTTTACAGAG ATGAAGTTTT TCATATCCAC AAGGCAGCAG AAGATCTGTT CGTCAACATT 
AGAGGCTACA ACAAAAGGAT AAATGACATC AGGGAGTGTA AAGAGAATGC AGTATCACAT 
GCAGGAGCAA CACACAGAGA AAGGCGCAAA TTTTTAAGGT CTGCTTTGAA AGAACTTGCT 
ACTGTACTCG CTGATCAGCC AGGCCTTCTG GGTCCCAAAG CACTTTTTGT ATTCATGGCA 
TTATCGTTTG CCCGTGATGA AATAATATGG CTACTTCGTC ATGCAGACAA CATTCCAAAG 
AAGGTCGCAG ATGACTTCAT GGATAAGCAC ATCGCTGAGC TCATATTTTA TATGGAAGAA 
CTGCGAGCGC ATGTACGGAA GTATGGGCCT GTGATGCAGA GATACTATGT GCAGTACCTG 
TCTGGCTTTG ATGCAGTTGT ACTAAATGAG CTAGTGCAGA ATCTTTCTGT GTGCCCTGAG 
GATGAATCAA TTATAATGTC TTCATTCGTA AATACAATGA CCTCCTTGAG TGTAAAACAA 
GTTGAAGACG GAGAGGTTTT TGACTTCAGA GGAATGAGAT TGGATTGGTT CAGATTGCAG 
GCCTATACCA GTGTTTCCAA AGCTTCATTG AGTCTTGCTG ACCACAGAGA ACTTGGAAAA 
ATGATGAACA CTATAATATT CCATACCAAA ATGGTGGATT CCTTAGTGGA GATGTTGGTG 
GAGACATCAG ACCTTTCCAT ATTTTGCTTT TACAGCCGAG CTTTTGAGAA GATGTTTCAG 
CAGTGTTTGG AGCTGCCATC CCAATCAAGA TATTCAATCT CCTTTCCTCT TATATGCACT 
CACTTTATGA GCTGCACTCA CGAACTTTGC CCTGAAGAGG TAATTTTTAT TAATTCCCAG 
CGGCATCACA TTGGAGACCG CAGTTTGTCA CTATGTAACA TGTTCTTGGA TGAAATGGCA 
AAGCAAGCTC GAAATCTAAT TACAGACATC TGCACGGAAC AGTGTACCCT TAGTGATCAG 
TTGCTGCCAA AGCACTGTGC CAAAACAATC AGTCAAGCGG TGAACAAAAA GTCAAAAAAG 
CAGACCGGAA AGAAAGGAGA ACCAGAAAGG GAAAAACCTG GAGTTGAAAG CATGAGAAAG 
AATAGACTTG TGGTAACAAA CCTGGATAAA TTGCACACTG CACTTTCAGA GCTCTGTTTC 
TCTATCAACT ATGCACCAAA TATGGTTGTA TGGGAACACA CATTTACCCC AAGAGAGTAT 
TTGACGTCGC ACTTGGAAAT CCGCTTTACC AAGTCTATTG TTGGCATGAC CATGTACAAT 
CAAGTCACTC AAGAGATTGC TAAACCTTCT GAGTTGTTAA CAAGTGTAAG AGCCTATATG 
ACTGTACTCC AATCAATAGA AAACTACGTG CAGATTGATA TCACAAGGGT ATTCAACAAT 
GTTCTACTTC AGCAAACACA ACATTTAGAC AGCCATGGAG AGCCAACAAT CACCAGTTTA 
TACACTAATT GGTATTTGGA AACACTACTG AGGCAAGTAA GCAATGGACA TATAGCATAT 
TTCCCAGCAA TGAAAGCTTT TGTAAACCTG CCAACTGAGA ATGAGCTGAC GTTTAATGCA 
GAAGAGTATT CCGACATCTC TGAAATGAGG GCATTGTCTG AGCTGCTGGG ACCATATGGC 
ATGAAGTTTC TCAGTGAAAG CCTCATGTGG CATATTTCCT CTCAGGTGGC TGAGCTGAAG 
AAACTTGTTG TGGAAAATGT GGAGGTCTTA ACCCAGATGA GAACAAGTTT TGATAAACCA 
GAGCAGATGG CAGCCCTTTT CAAAAGGCTT ACATCCGTTG ACAGTGTTTT GAAGAGGATG 
ACCATTATTG GAGTTATATT GTCTTTCCGA TCTCTTGCAC AGGAAGCACT AAGAGATGTT 
TTGTCTTACC ACATTCCTTT TCTTGTAAGC TCTGTTGAAG ACTTTAAGGA TCACATTCCT 
AGAGAGACAG ACATGAAGGT GGCTATGAAT GTGTATGAGT TATCATCAGC AGCTGGATTG 
CCATGTGAAA TAGACCCTGC CTTAGTTGTG GCACTGTCAT CTCAAAAATC TGAAACCATC 
AGCCCAGAGG AAGAATACAA AATAGCCTGT CTGCTTATGG TGTTTGTGGC TGTTTCTTTG 
CCAACATTGG CTAGTAACGT CATGTCGCAA TACAGCCCTG CAATTGAAGG TCATTGCAAC 
AACATACACT GCTTGGCAAA GGCAATTAAC CAAATTGCAG CAGCACTATT TACCATTCAC 
AAAGGGAGCA TCGAAGATCG TCTGAAAGAG TTCTTGGCGC TTGCATCCTC CAGCCTGCTA 
AAGATTGGCC AAGAGACTGA CAAAACTACT ACAAGAAACA GGGAATCTGT TTATTTGCTG 
CTAGATATGA TTGTTCAAGA ATCACCATTC CTGACCATGG ATCTGCTGGA GTCCTGCTTC 
CCATACGTCT TACTGAGAAA TGCATACCAT GCGGTTTACA AACAGAGTGT CACATCCTCT 
GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap1 ( XM_004917910.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917967.1 Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa16223
Clone ID Related Accession (Same CDS sequence)	XM_004917910.4 , XM_004917910.3
Accession Version	XM_004917910.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917910.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAAGCAA AGCCTTCTTA TCTGATCGAC AAAAATCTTG AATCTGCTGT CAAGTTTATT 
GTCCGGAAAT TTCCAGCAGT TGAAACCCGC AATAATAATC AACAGCTTGC ACAACTTCAA 
AAAGAGAAAT CTGAAATTTT GAAAAACCTG GCACTGTACT ACTTTACCTT TGTTGATGTT 
ATGGAATTTA AGGATCATGT ATGTGAGCTG CTAAACACTA TTGATGTCTG CCAGGTCTTC 
TTTGATATTA CGGTTAACTT TGATTTAACA AAGAACTATC TAGATCTGAT TGTAACCTAC 
ACAACACTAA TGATAATGCT TTCTCGGATT GAGGAGAGAA AAGCAATTAT AGGACTCTAC 
AACTACTCTC ATGAAATGAC CCATGGGGCA AGTGATCGGG AATATCCTCG GCTTGGACAG 
ATGATCGTTG ACTATGAAAA CCCTTTGAAG AAAATGATGG AGGAGTTTGT TCCCCATAGT 
AAGTCTCTAT CAGATGCCCT AATATCACTG CAAATGGTTT ATCCACGAAG GAATCTTTCA 
GCTGAGCAGT GGCGAAATGC TCAACTTCTA AGTCTCATCA GTGCTCCTAG TACAATGCTA 
AACCCAGCAC AATCGGACAC GATGCCATGC GAGTACCTTT CGCTTGATGC AATGGAGAAG 
TGGATTATTT TTGGATTTAT TTTGTGCCAT GGAATACTCA ACACTGATGC TACAGCATTG 
AATCTTTGGA AGCTCGCCCT TCAGAGCAGT TCATGTCTCT GTCTTTACAG AGATGAAGTT 
TTTCATATCC ACAAGGCAGC AGAAGATCTG TTCGTCAACA TTAGAGGCTA CAACAAAAGG 
ATAAATGACA TCAGGGAGTG TAAAGAGAAT GCAGTATCAC ATGCAGGAGC AACACACAGA 
GAAAGGCGCA AATTTTTAAG GTCTGCTTTG AAAGAACTTG CTACTGTACT CGCTGATCAG 
CCAGGCCTTC TGGGTCCCAA AGCACTTTTT GTATTCATGG CATTATCGTT TGCCCGTGAT 
GAAATAATAT GGCTACTTCG TCATGCAGAC AACATTCCAA AGAAGGTCGC AGATGACTTC 
ATGGATAAGC ACATCGCTGA GCTCATATTT TATATGGAAG AACTGCGAGC GCATGTACGG 
AAGTATGGGC CTGTGATGCA GAGATACTAT GTGCAGTACC TGTCTGGCTT TGATGCAGTT 
GTACTAAATG AGCTAGTGCA GAATCTTTCT GTGTGCCCTG AGGATGAATC AATTATAATG 
TCTTCATTCG TAAATACAAT GACCTCCTTG AGTGTAAAAC AAGTTGAAGA CGGAGAGGTT 
TTTGACTTCA GAGGAATGAG ATTGGATTGG TTCAGATTGC AGGCCTATAC CAGTGTTTCC 
AAAGCTTCAT TGAGTCTTGC TGACCACAGA GAACTTGGAA AAATGATGAA CACTATAATA 
TTCCATACCA AAATGGTGGA TTCCTTAGTG GAGATGTTGG TGGAGACATC AGACCTTTCC 
ATATTTTGCT TTTACAGCCG AGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTGCCA 
TCCCAATCAA GATATTCAAT CTCCTTTCCT CTTATATGCA CTCACTTTAT GAGCTGCACT 
CACGAACTTT GCCCTGAAGA GGTAATTTTT ATTAATTCCC AGCGGCATCA CATTGGAGAC 
CGCAGTTTGT CACTATGTAA CATGTTCTTG GATGAAATGG CAAAGCAAGC TCGAAATCTA 
ATTACAGACA TCTGCACGGA ACAGTGTACC CTTAGTGATC AGTTGCTGCC AAAGCACTGT 
GCCAAAACAA TCAGTCAAGC GGTGAACAAA AAGTCAAAAA AGCAGACCGG AAAGAAAGGA 
GAACCAGAAA GGGAAAAACC TGGAGTTGAA AGCATGAGAA AGAATAGACT TGTGGTAACA 
AACCTGGATA AATTGCACAC TGCACTTTCA GAGCTCTGTT TCTCTATCAA CTATGCACCA 
AATATGGTTG TATGGGAACA CACATTTACC CCAAGAGAGT ATTTGACGTC GCACTTGGAA 
ATCCGCTTTA CCAAGTCTAT TGTTGGCATG ACCATGTACA ATCAAGTCAC TCAAGAGATT 
GCTAAACCTT CTGAGTTGTT AACAAGTGTA AGAGCCTATA TGACTGTACT CCAATCAATA 
GAAAACTACG TGCAGATTGA TATCACAAGG GTATTCAACA ATGTTCTACT TCAGCAAACA 
CAACATTTAG ACAGCCATGG AGAGCCAACA ATCACCAGTT TATACACTAA TTGGTATTTG 
GAAACACTAC TGAGGCAAGT AAGCAATGGA CATATAGCAT ATTTCCCAGC AATGAAAGCT 
TTTGTAAACC TGCCAACTGA GAATGAGCTG ACGTTTAATG CAGAAGAGTA TTCCGACATC 
TCTGAAATGA GGGCATTGTC TGAGCTGCTG GGACCATATG GCATGAAGTT TCTCAGTGAA 
AGCCTCATGT GGCATATTTC CTCTCAGGTG GCTGAGCTGA AGAAACTTGT TGTGGAAAAT 
GTGGAGGTCT TAACCCAGAT GAGAACAAGT TTTGATAAAC CAGAGCAGAT GGCAGCCCTT 
TTCAAAAGGC TTACATCCGT TGACAGTGTT TTGAAGAGGA TGACCATTAT TGGAGTTATA 
TTGTCTTTCC GATCTCTTGC ACAGGAAGCA CTAAGAGATG TTTTGTCTTA CCACATTCCT 
TTTCTTGTAA GCTCTGTTGA AGACTTTAAG GATCACATTC CTAGAGAGAC AGACATGAAG 
GTGGCTATGA ATGTGTATGA GTTATCATCA GCAGCTGGAT TGCCATGTGA AATAGACCCT 
GCCTTAGTTG TGGCACTGTC ATCTCAAAAA TCTGAAACCA TCAGCCCAGA GGAAGAATAC 
AAAATAGCCT GTCTGCTTAT GGTGTTTGTG GCTGTTTCTT TGCCAACATT GGCTAGTAAC 
GTCATGTCGC AATACAGCCC TGCAATTGAA GGTCATTGCA ACAACATACA CTGCTTGGCA 
AAGGCAATTA ACCAAATTGC AGCAGCACTA TTTACCATTC ACAAAGGGAG CATCGAAGAT 
CGTCTGAAAG AGTTCTTGGC GCTTGCATCC TCCAGCCTGC TAAAGATTGG CCAAGAGACT 
GACAAAACTA CTACAAGAAA CAGGGAATCT GTTTATTTGC TGCTAGATAT GATTGTTCAA 
GAATCACCAT TCCTGACCAT GGATCTGCTG GAGTCCTGCT TCCCATACGT CTTACTGAGA 
AATGCATACC ATGCGGTTTA CAAACAGAGT GTCACATCCT CTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917967.1
CDS	295..3702
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIVTYTTLMIMLSRIEERKAIIGLYNYSHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSAEQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLCLYRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKENAVSHAGATHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIPKKVADDFMDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLICTHFMSCTHELCPEEVIFINSQRHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYAPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQVTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPEQMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSETISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_004917910.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917910.3

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAAGCAA AGCCTTCTTA TCTGATCGAC AAAAATCTTG AATCTGCTGT CAAGTTTATT 
GTCCGGAAAT TTCCAGCAGT TGAAACCCGC AATAATAATC AACAGCTTGC ACAACTTCAA 
AAAGAGAAAT CTGAAATTTT GAAAAACCTG GCACTGTACT ACTTTACCTT TGTTGATGTT 
ATGGAATTTA AGGATCATGT ATGTGAGCTG CTAAACACTA TTGATGTCTG CCAGGTCTTC 
TTTGATATTA CGGTTAACTT TGATTTAACA AAGAACTATC TAGATCTGAT TGTAACCTAC 
ACAACACTAA TGATAATGCT TTCTCGGATT GAGGAGAGAA AAGCAATTAT AGGACTCTAC 
AACTACTCTC ATGAAATGAC CCATGGGGCA AGTGATCGGG AATATCCTCG GCTTGGACAG 
ATGATCGTTG ACTATGAAAA CCCTTTGAAG AAAATGATGG AGGAGTTTGT TCCCCATAGT 
AAGTCTCTAT CAGATGCCCT AATATCACTG CAAATGGTTT ATCCACGAAG GAATCTTTCA 
GCTGAGCAGT GGCGAAATGC TCAACTTCTA AGTCTCATCA GTGCTCCTAG TACAATGCTA 
AACCCAGCAC AATCGGACAC GATGCCATGC GAGTACCTTT CGCTTGATGC AATGGAGAAG 
TGGATTATTT TTGGATTTAT TTTGTGCCAT GGAATACTCA ACACTGATGC TACAGCATTG 
AATCTTTGGA AGCTCGCCCT TCAGAGCAGT TCATGTCTCT GTCTTTACAG AGATGAAGTT 
TTTCATATCC ACAAGGCAGC AGAAGATCTG TTCGTCAACA TTAGAGGCTA CAACAAAAGG 
ATAAATGACA TCAGGGAGTG TAAAGAGAAT GCAGTATCAC ATGCAGGAGC AACACACAGA 
GAAAGGCGCA AATTTTTAAG GTCTGCTTTG AAAGAACTTG CTACTGTACT CGCTGATCAG 
CCAGGCCTTC TGGGTCCCAA AGCACTTTTT GTATTCATGG CATTATCGTT TGCCCGTGAT 
GAAATAATAT GGCTACTTCG TCATGCAGAC AACATTCCAA AGAAGGTCGC AGATGACTTC 
ATGGATAAGC ACATCGCTGA GCTCATATTT TATATGGAAG AACTGCGAGC GCATGTACGG 
AAGTATGGGC CTGTGATGCA GAGATACTAT GTGCAGTACC TGTCTGGCTT TGATGCAGTT 
GTACTAAATG AGCTAGTGCA GAATCTTTCT GTGTGCCCTG AGGATGAATC AATTATAATG 
TCTTCATTCG TAAATACAAT GACCTCCTTG AGTGTAAAAC AAGTTGAAGA CGGAGAGGTT 
TTTGACTTCA GAGGAATGAG ATTGGATTGG TTCAGATTGC AGGCCTATAC CAGTGTTTCC 
AAAGCTTCAT TGAGTCTTGC TGACCACAGA GAACTTGGAA AAATGATGAA CACTATAATA 
TTCCATACCA AAATGGTGGA TTCCTTAGTG GAGATGTTGG TGGAGACATC AGACCTTTCC 
ATATTTTGCT TTTACAGCCG AGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTGCCA 
TCCCAATCAA GATATTCAAT CTCCTTTCCT CTTATATGCA CTCACTTTAT GAGCTGCACT 
CACGAACTTT GCCCTGAAGA GGTAATTTTT ATTAATTCCC AGCGGCATCA CATTGGAGAC 
CGCAGTTTGT CACTATGTAA CATGTTCTTG GATGAAATGG CAAAGCAAGC TCGAAATCTA 
ATTACAGACA TCTGCACGGA ACAGTGTACC CTTAGTGATC AGTTGCTGCC AAAGCACTGT 
GCCAAAACAA TCAGTCAAGC GGTGAACAAA AAGTCAAAAA AGCAGACCGG AAAGAAAGGA 
GAACCAGAAA GGGAAAAACC TGGAGTTGAA AGCATGAGAA AGAATAGACT TGTGGTAACA 
AACCTGGATA AATTGCACAC TGCACTTTCA GAGCTCTGTT TCTCTATCAA CTATGCACCA 
AATATGGTTG TATGGGAACA CACATTTACC CCAAGAGAGT ATTTGACGTC GCACTTGGAA 
ATCCGCTTTA CCAAGTCTAT TGTTGGCATG ACCATGTACA ATCAAGTCAC TCAAGAGATT 
GCTAAACCTT CTGAGTTGTT AACAAGTGTA AGAGCCTATA TGACTGTACT CCAATCAATA 
GAAAACTACG TGCAGATTGA TATCACAAGG GTATTCAACA ATGTTCTACT TCAGCAAACA 
CAACATTTAG ACAGCCATGG AGAGCCAACA ATCACCAGTT TATACACTAA TTGGTATTTG 
GAAACACTAC TGAGGCAAGT AAGCAATGGA CATATAGCAT ATTTCCCAGC AATGAAAGCT 
TTTGTAAACC TGCCAACTGA GAATGAGCTG ACGTTTAATG CAGAAGAGTA TTCCGACATC 
TCTGAAATGA GGGCATTGTC TGAGCTGCTG GGACCATATG GCATGAAGTT TCTCAGTGAA 
AGCCTCATGT GGCATATTTC CTCTCAGGTG GCTGAGCTGA AGAAACTTGT TGTGGAAAAT 
GTGGAGGTCT TAACCCAGAT GAGAACAAGT TTTGATAAAC CAGAGCAGAT GGCAGCCCTT 
TTCAAAAGGC TTACATCCGT TGACAGTGTT TTGAAGAGGA TGACCATTAT TGGAGTTATA 
TTGTCTTTCC GATCTCTTGC ACAGGAAGCA CTAAGAGATG TTTTGTCTTA CCACATTCCT 
TTTCTTGTAA GCTCTGTTGA AGACTTTAAG GATCACATTC CTAGAGAGAC AGACATGAAG 
GTGGCTATGA ATGTGTATGA GTTATCATCA GCAGCTGGAT TGCCATGTGA AATAGACCCT 
GCCTTAGTTG TGGCACTGTC ATCTCAAAAA TCTGAAACCA TCAGCCCAGA GGAAGAATAC 
AAAATAGCCT GTCTGCTTAT GGTGTTTGTG GCTGTTTCTT TGCCAACATT GGCTAGTAAC 
GTCATGTCGC AATACAGCCC TGCAATTGAA GGTCATTGCA ACAACATACA CTGCTTGGCA 
AAGGCAATTA ACCAAATTGC AGCAGCACTA TTTACCATTC ACAAAGGGAG CATCGAAGAT 
CGTCTGAAAG AGTTCTTGGC GCTTGCATCC TCCAGCCTGC TAAAGATTGG CCAAGAGACT 
GACAAAACTA CTACAAGAAA CAGGGAATCT GTTTATTTGC TGCTAGATAT GATTGTTCAA 
GAATCACCAT TCCTGACCAT GGATCTGCTG GAGTCCTGCT TCCCATACGT CTTACTGAGA 
AATGCATACC ATGCGGTTTA CAAACAGAGT GTCACATCCT CTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap1 ( XM_002932013.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932059.1 Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa16224
Clone ID Related Accession (Same CDS sequence)	XM_002932013.4 , XM_002932013.5
Accession Version	XM_002932013.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932013.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGCA AGGACAAGTC 
TGGAAGGCAT GTGGAGACCC CAAAGCAAAG CCTTCTTATC TGATCGACAA AAATCTTGAA 
TCTGCTGTCA AGTTTATTGT CCGGAAATTT CCAGCAGTTG AAACCCGCAA TAATAATCAA 
CAGCTTGCAC AACTTCAAAA AGAGAAATCT GAAATTTTGA AAAACCTGGC ACTGTACTAC 
TTTACCTTTG TTGATGTTAT GGAATTTAAG GATCATGTAT GTGAGCTGCT AAACACTATT 
GATGTCTGCC AGGTCTTCTT TGATATTACG GTTAACTTTG ATTTAACAAA GAACTATCTA 
GATCTGATTG TAACCTACAC AACACTAATG ATAATGCTTT CTCGGATTGA GGAGAGAAAA 
GCAATTATAG GACTCTACAA CTACTCTCAT GAAATGACCC ATGGGGCAAG TGATCGGGAA 
TATCCTCGGC TTGGACAGAT GATCGTTGAC TATGAAAACC CTTTGAAGAA AATGATGGAG 
GAGTTTGTTC CCCATAGTAA GTCTCTATCA GATGCCCTAA TATCACTGCA AATGGTTTAT 
CCACGAAGGA ATCTTTCAGC TGAGCAGTGG CGAAATGCTC AACTTCTAAG TCTCATCAGT 
GCTCCTAGTA CAATGCTAAA CCCAGCACAA TCGGACACGA TGCCATGCGA GTACCTTTCG 
CTTGATGCAA TGGAGAAGTG GATTATTTTT GGATTTATTT TGTGCCATGG AATACTCAAC 
ACTGATGCTA CAGCATTGAA TCTTTGGAAG CTCGCCCTTC AGAGCAGTTC ATGTCTCTGT 
CTTTACAGAG ATGAAGTTTT TCATATCCAC AAGGCAGCAG AAGATCTGTT CGTCAACATT 
AGAGGCTACA ACAAAAGGAT AAATGACATC AGGGAGTGTA AAGAGAATGC AGTATCACAT 
GCAGGAGCAA CACACAGAGA AAGGCGCAAA TTTTTAAGGT CTGCTTTGAA AGAACTTGCT 
ACTGTACTCG CTGATCAGCC AGGCCTTCTG GGTCCCAAAG CACTTTTTGT ATTCATGGCA 
TTATCGTTTG CCCGTGATGA AATAATATGG CTACTTCGTC ATGCAGACAA CATTCCAAAG 
AAGGTCGCAG ATGACTTCAT GGATAAGCAC ATCGCTGAGC TCATATTTTA TATGGAAGAA 
CTGCGAGCGC ATGTACGGAA GTATGGGCCT GTGATGCAGA GATACTATGT GCAGTACCTG 
TCTGGCTTTG ATGCAGTTGT ACTAAATGAG CTAGTGCAGA ATCTTTCTGT GTGCCCTGAG 
GATGAATCAA TTATAATGTC TTCATTCGTA AATACAATGA CCTCCTTGAG TGTAAAACAA 
GTTGAAGACG GAGAGGTTTT TGACTTCAGA GGAATGAGAT TGGATTGGTT CAGATTGCAG 
GCCTATACCA GTGTTTCCAA AGCTTCATTG AGTCTTGCTG ACCACAGAGA ACTTGGAAAA 
ATGATGAACA CTATAATATT CCATACCAAA ATGGTGGATT CCTTAGTGGA GATGTTGGTG 
GAGACATCAG ACCTTTCCAT ATTTTGCTTT TACAGCCGAG CTTTTGAGAA GATGTTTCAG 
CAGTGTTTGG AGCTGCCATC CCAATCAAGA TATTCAATCT CCTTTCCTCT TATATGCACT 
CACTTTATGA GCTGCACTCA CGAACTTTGC CCTGAAGAGC GGCATCACAT TGGAGACCGC 
AGTTTGTCAC TATGTAACAT GTTCTTGGAT GAAATGGCAA AGCAAGCTCG AAATCTAATT 
ACAGACATCT GCACGGAACA GTGTACCCTT AGTGATCAGT TGCTGCCAAA GCACTGTGCC 
AAAACAATCA GTCAAGCGGT GAACAAAAAG TCAAAAAAGC AGACCGGAAA GAAAGGAGAA 
CCAGAAAGGG AAAAACCTGG AGTTGAAAGC ATGAGAAAGA ATAGACTTGT GGTAACAAAC 
CTGGATAAAT TGCACACTGC ACTTTCAGAG CTCTGTTTCT CTATCAACTA TGCACCAAAT 
ATGGTTGTAT GGGAACACAC ATTTACCCCA AGAGAGTATT TGACGTCGCA CTTGGAAATC 
CGCTTTACCA AGTCTATTGT TGGCATGACC ATGTACAATC AAGTCACTCA AGAGATTGCT 
AAACCTTCTG AGTTGTTAAC AAGTGTAAGA GCCTATATGA CTGTACTCCA ATCAATAGAA 
AACTACGTGC AGATTGATAT CACAAGGGTA TTCAACAATG TTCTACTTCA GCAAACACAA 
CATTTAGACA GCCATGGAGA GCCAACAATC ACCAGTTTAT ACACTAATTG GTATTTGGAA 
ACACTACTGA GGCAAGTAAG CAATGGACAT ATAGCATATT TCCCAGCAAT GAAAGCTTTT 
GTAAACCTGC CAACTGAGAA TGAGCTGACG TTTAATGCAG AAGAGTATTC CGACATCTCT 
GAAATGAGGG CATTGTCTGA GCTGCTGGGA CCATATGGCA TGAAGTTTCT CAGTGAAAGC 
CTCATGTGGC ATATTTCCTC TCAGGTGGCT GAGCTGAAGA AACTTGTTGT GGAAAATGTG 
GAGGTCTTAA CCCAGATGAG AACAAGTTTT GATAAACCAG AGCAGATGGC AGCCCTTTTC 
AAAAGGCTTA CATCCGTTGA CAGTGTTTTG AAGAGGATGA CCATTATTGG AGTTATATTG 
TCTTTCCGAT CTCTTGCACA GGAAGCACTA AGAGATGTTT TGTCTTACCA CATTCCTTTT 
CTTGTAAGCT CTGTTGAAGA CTTTAAGGAT CACATTCCTA GAGAGACAGA CATGAAGGTG 
GCTATGAATG TGTATGAGTT ATCATCAGCA GCTGGATTGC CATGTGAAAT AGACCCTGCC 
TTAGTTGTGG CACTGTCATC TCAAAAATCT GAAACCATCA GCCCAGAGGA AGAATACAAA 
ATAGCCTGTC TGCTTATGGT GTTTGTGGCT GTTTCTTTGC CAACATTGGC TAGTAACGTC 
ATGTCGCAAT ACAGCCCTGC AATTGAAGGT CATTGCAACA ACATACACTG CTTGGCAAAG 
GCAATTAACC AAATTGCAGC AGCACTATTT ACCATTCACA AAGGGAGCAT CGAAGATCGT 
CTGAAAGAGT TCTTGGCGCT TGCATCCTCC AGCCTGCTAA AGATTGGCCA AGAGACTGAC 
AAAACTACTA CAAGAAACAG GGAATCTGTT TATTTGCTGC TAGATATGAT TGTTCAAGAA 
TCACCATTCC TGACCATGGA TCTGCTGGAG TCCTGCTTCC CATACGTCTT ACTGAGAAAT 
GCATACCATG CGGTTTACAA ACAGAGTGTC ACATCCTCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932059.1
CDS	295..3699
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIVTYTTLMIMLSRIEERKAIIGLYNYSHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSAEQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLCLYRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKENAVSHAGATHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIPKKVADDFMDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLICTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYAPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQVTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPEQMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSETISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_002932013.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932013.4

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGCA AGGACAAGTC 
TGGAAGGCAT GTGGAGACCC CAAAGCAAAG CCTTCTTATC TGATCGACAA AAATCTTGAA 
TCTGCTGTCA AGTTTATTGT CCGGAAATTT CCAGCAGTTG AAACCCGCAA TAATAATCAA 
CAGCTTGCAC AACTTCAAAA AGAGAAATCT GAAATTTTGA AAAACCTGGC ACTGTACTAC 
TTTACCTTTG TTGATGTTAT GGAATTTAAG GATCATGTAT GTGAGCTGCT AAACACTATT 
GATGTCTGCC AGGTCTTCTT TGATATTACG GTTAACTTTG ATTTAACAAA GAACTATCTA 
GATCTGATTG TAACCTACAC AACACTAATG ATAATGCTTT CTCGGATTGA GGAGAGAAAA 
GCAATTATAG GACTCTACAA CTACTCTCAT GAAATGACCC ATGGGGCAAG TGATCGGGAA 
TATCCTCGGC TTGGACAGAT GATCGTTGAC TATGAAAACC CTTTGAAGAA AATGATGGAG 
GAGTTTGTTC CCCATAGTAA GTCTCTATCA GATGCCCTAA TATCACTGCA AATGGTTTAT 
CCACGAAGGA ATCTTTCAGC TGAGCAGTGG CGAAATGCTC AACTTCTAAG TCTCATCAGT 
GCTCCTAGTA CAATGCTAAA CCCAGCACAA TCGGACACGA TGCCATGCGA GTACCTTTCG 
CTTGATGCAA TGGAGAAGTG GATTATTTTT GGATTTATTT TGTGCCATGG AATACTCAAC 
ACTGATGCTA CAGCATTGAA TCTTTGGAAG CTCGCCCTTC AGAGCAGTTC ATGTCTCTGT 
CTTTACAGAG ATGAAGTTTT TCATATCCAC AAGGCAGCAG AAGATCTGTT CGTCAACATT 
AGAGGCTACA ACAAAAGGAT AAATGACATC AGGGAGTGTA AAGAGAATGC AGTATCACAT 
GCAGGAGCAA CACACAGAGA AAGGCGCAAA TTTTTAAGGT CTGCTTTGAA AGAACTTGCT 
ACTGTACTCG CTGATCAGCC AGGCCTTCTG GGTCCCAAAG CACTTTTTGT ATTCATGGCA 
TTATCGTTTG CCCGTGATGA AATAATATGG CTACTTCGTC ATGCAGACAA CATTCCAAAG 
AAGGTCGCAG ATGACTTCAT GGATAAGCAC ATCGCTGAGC TCATATTTTA TATGGAAGAA 
CTGCGAGCGC ATGTACGGAA GTATGGGCCT GTGATGCAGA GATACTATGT GCAGTACCTG 
TCTGGCTTTG ATGCAGTTGT ACTAAATGAG CTAGTGCAGA ATCTTTCTGT GTGCCCTGAG 
GATGAATCAA TTATAATGTC TTCATTCGTA AATACAATGA CCTCCTTGAG TGTAAAACAA 
GTTGAAGACG GAGAGGTTTT TGACTTCAGA GGAATGAGAT TGGATTGGTT CAGATTGCAG 
GCCTATACCA GTGTTTCCAA AGCTTCATTG AGTCTTGCTG ACCACAGAGA ACTTGGAAAA 
ATGATGAACA CTATAATATT CCATACCAAA ATGGTGGATT CCTTAGTGGA GATGTTGGTG 
GAGACATCAG ACCTTTCCAT ATTTTGCTTT TACAGCCGAG CTTTTGAGAA GATGTTTCAG 
CAGTGTTTGG AGCTGCCATC CCAATCAAGA TATTCAATCT CCTTTCCTCT TATATGCACT 
CACTTTATGA GCTGCACTCA CGAACTTTGC CCTGAAGAGC GGCATCACAT TGGAGACCGC 
AGTTTGTCAC TATGTAACAT GTTCTTGGAT GAAATGGCAA AGCAAGCTCG AAATCTAATT 
ACAGACATCT GCACGGAACA GTGTACCCTT AGTGATCAGT TGCTGCCAAA GCACTGTGCC 
AAAACAATCA GTCAAGCGGT GAACAAAAAG TCAAAAAAGC AGACCGGAAA GAAAGGAGAA 
CCAGAAAGGG AAAAACCTGG AGTTGAAAGC ATGAGAAAGA ATAGACTTGT GGTAACAAAC 
CTGGATAAAT TGCACACTGC ACTTTCAGAG CTCTGTTTCT CTATCAACTA TGCACCAAAT 
ATGGTTGTAT GGGAACACAC ATTTACCCCA AGAGAGTATT TGACGTCGCA CTTGGAAATC 
CGCTTTACCA AGTCTATTGT TGGCATGACC ATGTACAATC AAGTCACTCA AGAGATTGCT 
AAACCTTCTG AGTTGTTAAC AAGTGTAAGA GCCTATATGA CTGTACTCCA ATCAATAGAA 
AACTACGTGC AGATTGATAT CACAAGGGTA TTCAACAATG TTCTACTTCA GCAAACACAA 
CATTTAGACA GCCATGGAGA GCCAACAATC ACCAGTTTAT ACACTAATTG GTATTTGGAA 
ACACTACTGA GGCAAGTAAG CAATGGACAT ATAGCATATT TCCCAGCAAT GAAAGCTTTT 
GTAAACCTGC CAACTGAGAA TGAGCTGACG TTTAATGCAG AAGAGTATTC CGACATCTCT 
GAAATGAGGG CATTGTCTGA GCTGCTGGGA CCATATGGCA TGAAGTTTCT CAGTGAAAGC 
CTCATGTGGC ATATTTCCTC TCAGGTGGCT GAGCTGAAGA AACTTGTTGT GGAAAATGTG 
GAGGTCTTAA CCCAGATGAG AACAAGTTTT GATAAACCAG AGCAGATGGC AGCCCTTTTC 
AAAAGGCTTA CATCCGTTGA CAGTGTTTTG AAGAGGATGA CCATTATTGG AGTTATATTG 
TCTTTCCGAT CTCTTGCACA GGAAGCACTA AGAGATGTTT TGTCTTACCA CATTCCTTTT 
CTTGTAAGCT CTGTTGAAGA CTTTAAGGAT CACATTCCTA GAGAGACAGA CATGAAGGTG 
GCTATGAATG TGTATGAGTT ATCATCAGCA GCTGGATTGC CATGTGAAAT AGACCCTGCC 
TTAGTTGTGG CACTGTCATC TCAAAAATCT GAAACCATCA GCCCAGAGGA AGAATACAAA 
ATAGCCTGTC TGCTTATGGT GTTTGTGGCT GTTTCTTTGC CAACATTGGC TAGTAACGTC 
ATGTCGCAAT ACAGCCCTGC AATTGAAGGT CATTGCAACA ACATACACTG CTTGGCAAAG 
GCAATTAACC AAATTGCAGC AGCACTATTT ACCATTCACA AAGGGAGCAT CGAAGATCGT 
CTGAAAGAGT TCTTGGCGCT TGCATCCTCC AGCCTGCTAA AGATTGGCCA AGAGACTGAC 
AAAACTACTA CAAGAAACAG GGAATCTGTT TATTTGCTGC TAGATATGAT TGTTCAAGAA 
TCACCATTCC TGACCATGGA TCTGCTGGAG TCCTGCTTCC CATACGTCTT ACTGAGAAAT 
GCATACCATG CGGTTTACAA ACAGAGTGTC ACATCCTCTG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap1 ( XM_002932014.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932060.1 Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa16225
Clone ID Related Accession (Same CDS sequence)	XM_002932014.4 , XM_002932014.5
Accession Version	XM_002932014.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932014.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAAGCAA AGCCTTCTTA TCTGATCGAC AAAAATCTTG AATCTGCTGT CAAGTTTATT 
GTCCGGAAAT TTCCAGCAGT TGAAACCCGC AATAATAATC AACAGCTTGC ACAACTTCAA 
AAAGAGAAAT CTGAAATTTT GAAAAACCTG GCACTGTACT ACTTTACCTT TGTTGATGTT 
ATGGAATTTA AGGATCATGT ATGTGAGCTG CTAAACACTA TTGATGTCTG CCAGGTCTTC 
TTTGATATTA CGGTTAACTT TGATTTAACA AAGAACTATC TAGATCTGAT TGTAACCTAC 
ACAACACTAA TGATAATGCT TTCTCGGATT GAGGAGAGAA AAGCAATTAT AGGACTCTAC 
AACTACTCTC ATGAAATGAC CCATGGGGCA AGTGATCGGG AATATCCTCG GCTTGGACAG 
ATGATCGTTG ACTATGAAAA CCCTTTGAAG AAAATGATGG AGGAGTTTGT TCCCCATAGT 
AAGTCTCTAT CAGATGCCCT AATATCACTG CAAATGGTTT ATCCACGAAG GAATCTTTCA 
GCTGAGCAGT GGCGAAATGC TCAACTTCTA AGTCTCATCA GTGCTCCTAG TACAATGCTA 
AACCCAGCAC AATCGGACAC GATGCCATGC GAGTACCTTT CGCTTGATGC AATGGAGAAG 
TGGATTATTT TTGGATTTAT TTTGTGCCAT GGAATACTCA ACACTGATGC TACAGCATTG 
AATCTTTGGA AGCTCGCCCT TCAGAGCAGT TCATGTCTCT GTCTTTACAG AGATGAAGTT 
TTTCATATCC ACAAGGCAGC AGAAGATCTG TTCGTCAACA TTAGAGGCTA CAACAAAAGG 
ATAAATGACA TCAGGGAGTG TAAAGAGAAT GCAGTATCAC ATGCAGGAGC AACACACAGA 
GAAAGGCGCA AATTTTTAAG GTCTGCTTTG AAAGAACTTG CTACTGTACT CGCTGATCAG 
CCAGGCCTTC TGGGTCCCAA AGCACTTTTT GTATTCATGG CATTATCGTT TGCCCGTGAT 
GAAATAATAT GGCTACTTCG TCATGCAGAC AACATTCCAA AGAAGGTCGC AGATGACTTC 
ATGGATAAGC ACATCGCTGA GCTCATATTT TATATGGAAG AACTGCGAGC GCATGTACGG 
AAGTATGGGC CTGTGATGCA GAGATACTAT GTGCAGTACC TGTCTGGCTT TGATGCAGTT 
GTACTAAATG AGCTAGTGCA GAATCTTTCT GTGTGCCCTG AGGATGAATC AATTATAATG 
TCTTCATTCG TAAATACAAT GACCTCCTTG AGTGTAAAAC AAGTTGAAGA CGGAGAGGTT 
TTTGACTTCA GAGGAATGAG ATTGGATTGG TTCAGATTGC AGGCCTATAC CAGTGTTTCC 
AAAGCTTCAT TGAGTCTTGC TGACCACAGA GAACTTGGAA AAATGATGAA CACTATAATA 
TTCCATACCA AAATGGTGGA TTCCTTAGTG GAGATGTTGG TGGAGACATC AGACCTTTCC 
ATATTTTGCT TTTACAGCCG AGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTGCCA 
TCCCAATCAA GATATTCAAT CTCCTTTCCT CTTATATGCA CTCACTTTAT GAGCTGCACT 
CACGAACTTT GCCCTGAAGA GCGGCATCAC ATTGGAGACC GCAGTTTGTC ACTATGTAAC 
ATGTTCTTGG ATGAAATGGC AAAGCAAGCT CGAAATCTAA TTACAGACAT CTGCACGGAA 
CAGTGTACCC TTAGTGATCA GTTGCTGCCA AAGCACTGTG CCAAAACAAT CAGTCAAGCG 
GTGAACAAAA AGTCAAAAAA GCAGACCGGA AAGAAAGGAG AACCAGAAAG GGAAAAACCT 
GGAGTTGAAA GCATGAGAAA GAATAGACTT GTGGTAACAA ACCTGGATAA ATTGCACACT 
GCACTTTCAG AGCTCTGTTT CTCTATCAAC TATGCACCAA ATATGGTTGT ATGGGAACAC 
ACATTTACCC CAAGAGAGTA TTTGACGTCG CACTTGGAAA TCCGCTTTAC CAAGTCTATT 
GTTGGCATGA CCATGTACAA TCAAGTCACT CAAGAGATTG CTAAACCTTC TGAGTTGTTA 
ACAAGTGTAA GAGCCTATAT GACTGTACTC CAATCAATAG AAAACTACGT GCAGATTGAT 
ATCACAAGGG TATTCAACAA TGTTCTACTT CAGCAAACAC AACATTTAGA CAGCCATGGA 
GAGCCAACAA TCACCAGTTT ATACACTAAT TGGTATTTGG AAACACTACT GAGGCAAGTA 
AGCAATGGAC ATATAGCATA TTTCCCAGCA ATGAAAGCTT TTGTAAACCT GCCAACTGAG 
AATGAGCTGA CGTTTAATGC AGAAGAGTAT TCCGACATCT CTGAAATGAG GGCATTGTCT 
GAGCTGCTGG GACCATATGG CATGAAGTTT CTCAGTGAAA GCCTCATGTG GCATATTTCC 
TCTCAGGTGG CTGAGCTGAA GAAACTTGTT GTGGAAAATG TGGAGGTCTT AACCCAGATG 
AGAACAAGTT TTGATAAACC AGAGCAGATG GCAGCCCTTT TCAAAAGGCT TACATCCGTT 
GACAGTGTTT TGAAGAGGAT GACCATTATT GGAGTTATAT TGTCTTTCCG ATCTCTTGCA 
CAGGAAGCAC TAAGAGATGT TTTGTCTTAC CACATTCCTT TTCTTGTAAG CTCTGTTGAA 
GACTTTAAGG ATCACATTCC TAGAGAGACA GACATGAAGG TGGCTATGAA TGTGTATGAG 
TTATCATCAG CAGCTGGATT GCCATGTGAA ATAGACCCTG CCTTAGTTGT GGCACTGTCA 
TCTCAAAAAT CTGAAACCAT CAGCCCAGAG GAAGAATACA AAATAGCCTG TCTGCTTATG 
GTGTTTGTGG CTGTTTCTTT GCCAACATTG GCTAGTAACG TCATGTCGCA ATACAGCCCT 
GCAATTGAAG GTCATTGCAA CAACATACAC TGCTTGGCAA AGGCAATTAA CCAAATTGCA 
GCAGCACTAT TTACCATTCA CAAAGGGAGC ATCGAAGATC GTCTGAAAGA GTTCTTGGCG 
CTTGCATCCT CCAGCCTGCT AAAGATTGGC CAAGAGACTG ACAAAACTAC TACAAGAAAC 
AGGGAATCTG TTTATTTGCT GCTAGATATG ATTGTTCAAG AATCACCATT CCTGACCATG 
GATCTGCTGG AGTCCTGCTT CCCATACGTC TTACTGAGAA ATGCATACCA TGCGGTTTAC 
AAACAGAGTG TCACATCCTC TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932060.1
CDS	295..3681
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIVTYTTLMIMLSRIEERKAIIGLYNYSHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSAEQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLCLYRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKENAVSHAGATHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIPKKVADDFMDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLICTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYAPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQVTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPEQMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSETISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_002932014.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932014.4

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAAGCAA AGCCTTCTTA TCTGATCGAC AAAAATCTTG AATCTGCTGT CAAGTTTATT 
GTCCGGAAAT TTCCAGCAGT TGAAACCCGC AATAATAATC AACAGCTTGC ACAACTTCAA 
AAAGAGAAAT CTGAAATTTT GAAAAACCTG GCACTGTACT ACTTTACCTT TGTTGATGTT 
ATGGAATTTA AGGATCATGT ATGTGAGCTG CTAAACACTA TTGATGTCTG CCAGGTCTTC 
TTTGATATTA CGGTTAACTT TGATTTAACA AAGAACTATC TAGATCTGAT TGTAACCTAC 
ACAACACTAA TGATAATGCT TTCTCGGATT GAGGAGAGAA AAGCAATTAT AGGACTCTAC 
AACTACTCTC ATGAAATGAC CCATGGGGCA AGTGATCGGG AATATCCTCG GCTTGGACAG 
ATGATCGTTG ACTATGAAAA CCCTTTGAAG AAAATGATGG AGGAGTTTGT TCCCCATAGT 
AAGTCTCTAT CAGATGCCCT AATATCACTG CAAATGGTTT ATCCACGAAG GAATCTTTCA 
GCTGAGCAGT GGCGAAATGC TCAACTTCTA AGTCTCATCA GTGCTCCTAG TACAATGCTA 
AACCCAGCAC AATCGGACAC GATGCCATGC GAGTACCTTT CGCTTGATGC AATGGAGAAG 
TGGATTATTT TTGGATTTAT TTTGTGCCAT GGAATACTCA ACACTGATGC TACAGCATTG 
AATCTTTGGA AGCTCGCCCT TCAGAGCAGT TCATGTCTCT GTCTTTACAG AGATGAAGTT 
TTTCATATCC ACAAGGCAGC AGAAGATCTG TTCGTCAACA TTAGAGGCTA CAACAAAAGG 
ATAAATGACA TCAGGGAGTG TAAAGAGAAT GCAGTATCAC ATGCAGGAGC AACACACAGA 
GAAAGGCGCA AATTTTTAAG GTCTGCTTTG AAAGAACTTG CTACTGTACT CGCTGATCAG 
CCAGGCCTTC TGGGTCCCAA AGCACTTTTT GTATTCATGG CATTATCGTT TGCCCGTGAT 
GAAATAATAT GGCTACTTCG TCATGCAGAC AACATTCCAA AGAAGGTCGC AGATGACTTC 
ATGGATAAGC ACATCGCTGA GCTCATATTT TATATGGAAG AACTGCGAGC GCATGTACGG 
AAGTATGGGC CTGTGATGCA GAGATACTAT GTGCAGTACC TGTCTGGCTT TGATGCAGTT 
GTACTAAATG AGCTAGTGCA GAATCTTTCT GTGTGCCCTG AGGATGAATC AATTATAATG 
TCTTCATTCG TAAATACAAT GACCTCCTTG AGTGTAAAAC AAGTTGAAGA CGGAGAGGTT 
TTTGACTTCA GAGGAATGAG ATTGGATTGG TTCAGATTGC AGGCCTATAC CAGTGTTTCC 
AAAGCTTCAT TGAGTCTTGC TGACCACAGA GAACTTGGAA AAATGATGAA CACTATAATA 
TTCCATACCA AAATGGTGGA TTCCTTAGTG GAGATGTTGG TGGAGACATC AGACCTTTCC 
ATATTTTGCT TTTACAGCCG AGCTTTTGAG AAGATGTTTC AGCAGTGTTT GGAGCTGCCA 
TCCCAATCAA GATATTCAAT CTCCTTTCCT CTTATATGCA CTCACTTTAT GAGCTGCACT 
CACGAACTTT GCCCTGAAGA GCGGCATCAC ATTGGAGACC GCAGTTTGTC ACTATGTAAC 
ATGTTCTTGG ATGAAATGGC AAAGCAAGCT CGAAATCTAA TTACAGACAT CTGCACGGAA 
CAGTGTACCC TTAGTGATCA GTTGCTGCCA AAGCACTGTG CCAAAACAAT CAGTCAAGCG 
GTGAACAAAA AGTCAAAAAA GCAGACCGGA AAGAAAGGAG AACCAGAAAG GGAAAAACCT 
GGAGTTGAAA GCATGAGAAA GAATAGACTT GTGGTAACAA ACCTGGATAA ATTGCACACT 
GCACTTTCAG AGCTCTGTTT CTCTATCAAC TATGCACCAA ATATGGTTGT ATGGGAACAC 
ACATTTACCC CAAGAGAGTA TTTGACGTCG CACTTGGAAA TCCGCTTTAC CAAGTCTATT 
GTTGGCATGA CCATGTACAA TCAAGTCACT CAAGAGATTG CTAAACCTTC TGAGTTGTTA 
ACAAGTGTAA GAGCCTATAT GACTGTACTC CAATCAATAG AAAACTACGT GCAGATTGAT 
ATCACAAGGG TATTCAACAA TGTTCTACTT CAGCAAACAC AACATTTAGA CAGCCATGGA 
GAGCCAACAA TCACCAGTTT ATACACTAAT TGGTATTTGG AAACACTACT GAGGCAAGTA 
AGCAATGGAC ATATAGCATA TTTCCCAGCA ATGAAAGCTT TTGTAAACCT GCCAACTGAG 
AATGAGCTGA CGTTTAATGC AGAAGAGTAT TCCGACATCT CTGAAATGAG GGCATTGTCT 
GAGCTGCTGG GACCATATGG CATGAAGTTT CTCAGTGAAA GCCTCATGTG GCATATTTCC 
TCTCAGGTGG CTGAGCTGAA GAAACTTGTT GTGGAAAATG TGGAGGTCTT AACCCAGATG 
AGAACAAGTT TTGATAAACC AGAGCAGATG GCAGCCCTTT TCAAAAGGCT TACATCCGTT 
GACAGTGTTT TGAAGAGGAT GACCATTATT GGAGTTATAT TGTCTTTCCG ATCTCTTGCA 
CAGGAAGCAC TAAGAGATGT TTTGTCTTAC CACATTCCTT TTCTTGTAAG CTCTGTTGAA 
GACTTTAAGG ATCACATTCC TAGAGAGACA GACATGAAGG TGGCTATGAA TGTGTATGAG 
TTATCATCAG CAGCTGGATT GCCATGTGAA ATAGACCCTG CCTTAGTTGT GGCACTGTCA 
TCTCAAAAAT CTGAAACCAT CAGCCCAGAG GAAGAATACA AAATAGCCTG TCTGCTTATG 
GTGTTTGTGG CTGTTTCTTT GCCAACATTG GCTAGTAACG TCATGTCGCA ATACAGCCCT 
GCAATTGAAG GTCATTGCAA CAACATACAC TGCTTGGCAA AGGCAATTAA CCAAATTGCA 
GCAGCACTAT TTACCATTCA CAAAGGGAGC ATCGAAGATC GTCTGAAAGA GTTCTTGGCG 
CTTGCATCCT CCAGCCTGCT AAAGATTGGC CAAGAGACTG ACAAAACTAC TACAAGAAAC 
AGGGAATCTG TTTATTTGCT GCTAGATATG ATTGTTCAAG AATCACCATT CCTGACCATG 
GATCTGCTGG AGTCCTGCTT CCCATACGTC TTACTGAGAA ATGCATACCA TGCGGTTTAC 
AAACAGAGTG TCACATCCTC TGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nckap1 ( XM_004917911.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917968.1 Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X5, mRNA.

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CloneID	OXa16226
Clone ID Related Accession (Same CDS sequence)	XM_004917911.3 , XM_004917911.4
Accession Version	XM_004917911.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	nck-associated protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030685.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004917911.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAAGCAA AGCCTTCTTA TCTGATCGAC AAAAATCTTG AATCTGCTGT CAAGTTTATT 
GTCCGGAAAT TTCCAGCAGT TGAAACCCGC AATAATAATC TTGCACAACT TCAAAAAGAG 
AAATCTGAAA TTTTGAAAAA CCTGGCACTG TACTACTTTA CCTTTGTTGA TGTTATGGAA 
TTTAAGGATC ATGTATGTGA GCTGCTAAAC ACTATTGATG TCTGCCAGGT CTTCTTTGAT 
ATTACGGTTA ACTTTGATTT AACAAAGAAC TATCTAGATC TGATTGTAAC CTACACAACA 
CTAATGATAA TGCTTTCTCG GATTGAGGAG AGAAAAGCAA TTATAGGACT CTACAACTAC 
TCTCATGAAA TGACCCATGG GGCAAGTGAT CGGGAATATC CTCGGCTTGG ACAGATGATC 
GTTGACTATG AAAACCCTTT GAAGAAAATG ATGGAGGAGT TTGTTCCCCA TAGTAAGTCT 
CTATCAGATG CCCTAATATC ACTGCAAATG GTTTATCCAC GAAGGAATCT TTCAGCTGAG 
CAGTGGCGAA ATGCTCAACT TCTAAGTCTC ATCAGTGCTC CTAGTACAAT GCTAAACCCA 
GCACAATCGG ACACGATGCC ATGCGAGTAC CTTTCGCTTG ATGCAATGGA GAAGTGGATT 
ATTTTTGGAT TTATTTTGTG CCATGGAATA CTCAACACTG ATGCTACAGC ATTGAATCTT 
TGGAAGCTCG CCCTTCAGAG CAGTTCATGT CTCTGTCTTT ACAGAGATGA AGTTTTTCAT 
ATCCACAAGG CAGCAGAAGA TCTGTTCGTC AACATTAGAG GCTACAACAA AAGGATAAAT 
GACATCAGGG AGTGTAAAGA GAATGCAGTA TCACATGCAG GAGCAACACA CAGAGAAAGG 
CGCAAATTTT TAAGGTCTGC TTTGAAAGAA CTTGCTACTG TACTCGCTGA TCAGCCAGGC 
CTTCTGGGTC CCAAAGCACT TTTTGTATTC ATGGCATTAT CGTTTGCCCG TGATGAAATA 
ATATGGCTAC TTCGTCATGC AGACAACATT CCAAAGAAGG TCGCAGATGA CTTCATGGAT 
AAGCACATCG CTGAGCTCAT ATTTTATATG GAAGAACTGC GAGCGCATGT ACGGAAGTAT 
GGGCCTGTGA TGCAGAGATA CTATGTGCAG TACCTGTCTG GCTTTGATGC AGTTGTACTA 
AATGAGCTAG TGCAGAATCT TTCTGTGTGC CCTGAGGATG AATCAATTAT AATGTCTTCA 
TTCGTAAATA CAATGACCTC CTTGAGTGTA AAACAAGTTG AAGACGGAGA GGTTTTTGAC 
TTCAGAGGAA TGAGATTGGA TTGGTTCAGA TTGCAGGCCT ATACCAGTGT TTCCAAAGCT 
TCATTGAGTC TTGCTGACCA CAGAGAACTT GGAAAAATGA TGAACACTAT AATATTCCAT 
ACCAAAATGG TGGATTCCTT AGTGGAGATG TTGGTGGAGA CATCAGACCT TTCCATATTT 
TGCTTTTACA GCCGAGCTTT TGAGAAGATG TTTCAGCAGT GTTTGGAGCT GCCATCCCAA 
TCAAGATATT CAATCTCCTT TCCTCTTATA TGCACTCACT TTATGAGCTG CACTCACGAA 
CTTTGCCCTG AAGAGCGGCA TCACATTGGA GACCGCAGTT TGTCACTATG TAACATGTTC 
TTGGATGAAA TGGCAAAGCA AGCTCGAAAT CTAATTACAG ACATCTGCAC GGAACAGTGT 
ACCCTTAGTG ATCAGTTGCT GCCAAAGCAC TGTGCCAAAA CAATCAGTCA AGCGGTGAAC 
AAAAAGTCAA AAAAGCAGAC CGGAAAGAAA GGAGAACCAG AAAGGGAAAA ACCTGGAGTT 
GAAAGCATGA GAAAGAATAG ACTTGTGGTA ACAAACCTGG ATAAATTGCA CACTGCACTT 
TCAGAGCTCT GTTTCTCTAT CAACTATGCA CCAAATATGG TTGTATGGGA ACACACATTT 
ACCCCAAGAG AGTATTTGAC GTCGCACTTG GAAATCCGCT TTACCAAGTC TATTGTTGGC 
ATGACCATGT ACAATCAAGT CACTCAAGAG ATTGCTAAAC CTTCTGAGTT GTTAACAAGT 
GTAAGAGCCT ATATGACTGT ACTCCAATCA ATAGAAAACT ACGTGCAGAT TGATATCACA 
AGGGTATTCA ACAATGTTCT ACTTCAGCAA ACACAACATT TAGACAGCCA TGGAGAGCCA 
ACAATCACCA GTTTATACAC TAATTGGTAT TTGGAAACAC TACTGAGGCA AGTAAGCAAT 
GGACATATAG CATATTTCCC AGCAATGAAA GCTTTTGTAA ACCTGCCAAC TGAGAATGAG 
CTGACGTTTA ATGCAGAAGA GTATTCCGAC ATCTCTGAAA TGAGGGCATT GTCTGAGCTG 
CTGGGACCAT ATGGCATGAA GTTTCTCAGT GAAAGCCTCA TGTGGCATAT TTCCTCTCAG 
GTGGCTGAGC TGAAGAAACT TGTTGTGGAA AATGTGGAGG TCTTAACCCA GATGAGAACA 
AGTTTTGATA AACCAGAGCA GATGGCAGCC CTTTTCAAAA GGCTTACATC CGTTGACAGT 
GTTTTGAAGA GGATGACCAT TATTGGAGTT ATATTGTCTT TCCGATCTCT TGCACAGGAA 
GCACTAAGAG ATGTTTTGTC TTACCACATT CCTTTTCTTG TAAGCTCTGT TGAAGACTTT 
AAGGATCACA TTCCTAGAGA GACAGACATG AAGGTGGCTA TGAATGTGTA TGAGTTATCA 
TCAGCAGCTG GATTGCCATG TGAAATAGAC CCTGCCTTAG TTGTGGCACT GTCATCTCAA 
AAATCTGAAA CCATCAGCCC AGAGGAAGAA TACAAAATAG CCTGTCTGCT TATGGTGTTT 
GTGGCTGTTT CTTTGCCAAC ATTGGCTAGT AACGTCATGT CGCAATACAG CCCTGCAATT 
GAAGGTCATT GCAACAACAT ACACTGCTTG GCAAAGGCAA TTAACCAAAT TGCAGCAGCA 
CTATTTACCA TTCACAAAGG GAGCATCGAA GATCGTCTGA AAGAGTTCTT GGCGCTTGCA 
TCCTCCAGCC TGCTAAAGAT TGGCCAAGAG ACTGACAAAA CTACTACAAG AAACAGGGAA 
TCTGTTTATT TGCTGCTAGA TATGATTGTT CAAGAATCAC CATTCCTGAC CATGGATCTG 
CTGGAGTCCT GCTTCCCATA CGTCTTACTG AGAAATGCAT ACCATGCGGT TTACAAACAG 
AGTGTCACAT CCTCTGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917968.1
CDS	168..3548
Translation	MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIVTYTTLMIMLSRIEERKAIIGLYNYSHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSAEQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLCLYRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKENAVSHAGATHRERRKFLRSALKELATVLADQPGLLGPKALFVFMALSFARDEIIWLLRHADNIPKKVADDFMDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSISFPLICTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYAPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQVTQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPEQMAALFKRLTSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSVEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSETISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_004917911.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NCK-associated protein 1 (nckap1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004917911.3

ORF Insert Sequence:

ATGTCGCGCT CCGTGCTGCA GCCCAGTCAG CAGAAGCTGG CGGAGAAGCT CACTATCCTC 
AACGACAGGG GGGTCGGGAT GCTCACCCGT CTGTACAACA TCAAGAAGGC ATGTGGAGAC 
CCCAAAGCAA AGCCTTCTTA TCTGATCGAC AAAAATCTTG AATCTGCTGT CAAGTTTATT 
GTCCGGAAAT TTCCAGCAGT TGAAACCCGC AATAATAATC TTGCACAACT TCAAAAAGAG 
AAATCTGAAA TTTTGAAAAA CCTGGCACTG TACTACTTTA CCTTTGTTGA TGTTATGGAA 
TTTAAGGATC ATGTATGTGA GCTGCTAAAC ACTATTGATG TCTGCCAGGT CTTCTTTGAT 
ATTACGGTTA ACTTTGATTT AACAAAGAAC TATCTAGATC TGATTGTAAC CTACACAACA 
CTAATGATAA TGCTTTCTCG GATTGAGGAG AGAAAAGCAA TTATAGGACT CTACAACTAC 
TCTCATGAAA TGACCCATGG GGCAAGTGAT CGGGAATATC CTCGGCTTGG ACAGATGATC 
GTTGACTATG AAAACCCTTT GAAGAAAATG ATGGAGGAGT TTGTTCCCCA TAGTAAGTCT 
CTATCAGATG CCCTAATATC ACTGCAAATG GTTTATCCAC GAAGGAATCT TTCAGCTGAG 
CAGTGGCGAA ATGCTCAACT TCTAAGTCTC ATCAGTGCTC CTAGTACAAT GCTAAACCCA 
GCACAATCGG ACACGATGCC ATGCGAGTAC CTTTCGCTTG ATGCAATGGA GAAGTGGATT 
ATTTTTGGAT TTATTTTGTG CCATGGAATA CTCAACACTG ATGCTACAGC ATTGAATCTT 
TGGAAGCTCG CCCTTCAGAG CAGTTCATGT CTCTGTCTTT ACAGAGATGA AGTTTTTCAT 
ATCCACAAGG CAGCAGAAGA TCTGTTCGTC AACATTAGAG GCTACAACAA AAGGATAAAT 
GACATCAGGG AGTGTAAAGA GAATGCAGTA TCACATGCAG GAGCAACACA CAGAGAAAGG 
CGCAAATTTT TAAGGTCTGC TTTGAAAGAA CTTGCTACTG TACTCGCTGA TCAGCCAGGC 
CTTCTGGGTC CCAAAGCACT TTTTGTATTC ATGGCATTAT CGTTTGCCCG TGATGAAATA 
ATATGGCTAC TTCGTCATGC AGACAACATT CCAAAGAAGG TCGCAGATGA CTTCATGGAT 
AAGCACATCG CTGAGCTCAT ATTTTATATG GAAGAACTGC GAGCGCATGT ACGGAAGTAT 
GGGCCTGTGA TGCAGAGATA CTATGTGCAG TACCTGTCTG GCTTTGATGC AGTTGTACTA 
AATGAGCTAG TGCAGAATCT TTCTGTGTGC CCTGAGGATG AATCAATTAT AATGTCTTCA 
TTCGTAAATA CAATGACCTC CTTGAGTGTA AAACAAGTTG AAGACGGAGA GGTTTTTGAC 
TTCAGAGGAA TGAGATTGGA TTGGTTCAGA TTGCAGGCCT ATACCAGTGT TTCCAAAGCT 
TCATTGAGTC TTGCTGACCA CAGAGAACTT GGAAAAATGA TGAACACTAT AATATTCCAT 
ACCAAAATGG TGGATTCCTT AGTGGAGATG TTGGTGGAGA CATCAGACCT TTCCATATTT 
TGCTTTTACA GCCGAGCTTT TGAGAAGATG TTTCAGCAGT GTTTGGAGCT GCCATCCCAA 
TCAAGATATT CAATCTCCTT TCCTCTTATA TGCACTCACT TTATGAGCTG CACTCACGAA 
CTTTGCCCTG AAGAGCGGCA TCACATTGGA GACCGCAGTT TGTCACTATG TAACATGTTC 
TTGGATGAAA TGGCAAAGCA AGCTCGAAAT CTAATTACAG ACATCTGCAC GGAACAGTGT 
ACCCTTAGTG ATCAGTTGCT GCCAAAGCAC TGTGCCAAAA CAATCAGTCA AGCGGTGAAC 
AAAAAGTCAA AAAAGCAGAC CGGAAAGAAA GGAGAACCAG AAAGGGAAAA ACCTGGAGTT 
GAAAGCATGA GAAAGAATAG ACTTGTGGTA ACAAACCTGG ATAAATTGCA CACTGCACTT 
TCAGAGCTCT GTTTCTCTAT CAACTATGCA CCAAATATGG TTGTATGGGA ACACACATTT 
ACCCCAAGAG AGTATTTGAC GTCGCACTTG GAAATCCGCT TTACCAAGTC TATTGTTGGC 
ATGACCATGT ACAATCAAGT CACTCAAGAG ATTGCTAAAC CTTCTGAGTT GTTAACAAGT 
GTAAGAGCCT ATATGACTGT ACTCCAATCA ATAGAAAACT ACGTGCAGAT TGATATCACA 
AGGGTATTCA ACAATGTTCT ACTTCAGCAA ACACAACATT TAGACAGCCA TGGAGAGCCA 
ACAATCACCA GTTTATACAC TAATTGGTAT TTGGAAACAC TACTGAGGCA AGTAAGCAAT 
GGACATATAG CATATTTCCC AGCAATGAAA GCTTTTGTAA ACCTGCCAAC TGAGAATGAG 
CTGACGTTTA ATGCAGAAGA GTATTCCGAC ATCTCTGAAA TGAGGGCATT GTCTGAGCTG 
CTGGGACCAT ATGGCATGAA GTTTCTCAGT GAAAGCCTCA TGTGGCATAT TTCCTCTCAG 
GTGGCTGAGC TGAAGAAACT TGTTGTGGAA AATGTGGAGG TCTTAACCCA GATGAGAACA 
AGTTTTGATA AACCAGAGCA GATGGCAGCC CTTTTCAAAA GGCTTACATC CGTTGACAGT 
GTTTTGAAGA GGATGACCAT TATTGGAGTT ATATTGTCTT TCCGATCTCT TGCACAGGAA 
GCACTAAGAG ATGTTTTGTC TTACCACATT CCTTTTCTTG TAAGCTCTGT TGAAGACTTT 
AAGGATCACA TTCCTAGAGA GACAGACATG AAGGTGGCTA TGAATGTGTA TGAGTTATCA 
TCAGCAGCTG GATTGCCATG TGAAATAGAC CCTGCCTTAG TTGTGGCACT GTCATCTCAA 
AAATCTGAAA CCATCAGCCC AGAGGAAGAA TACAAAATAG CCTGTCTGCT TATGGTGTTT 
GTGGCTGTTT CTTTGCCAAC ATTGGCTAGT AACGTCATGT CGCAATACAG CCCTGCAATT 
GAAGGTCATT GCAACAACAT ACACTGCTTG GCAAAGGCAA TTAACCAAAT TGCAGCAGCA 
CTATTTACCA TTCACAAAGG GAGCATCGAA GATCGTCTGA AAGAGTTCTT GGCGCTTGCA 
TCCTCCAGCC TGCTAAAGAT TGGCCAAGAG ACTGACAAAA CTACTACAAG AAACAGGGAA 
TCTGTTTATT TGCTGCTAGA TATGATTGTT CAAGAATCAC CATTCCTGAC CATGGATCTG 
CTGGAGTCCT GCTTCCCATA CGTCTTACTG AGAAATGCAT ACCATGCGGT TTACAAACAG 
AGTGTCACAT CCTCTGCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	nckap1
Entrez Gene ID	100379683
Full Name	NCK-associated protein 1
General protein information	Preferred Names NCK-associated protein 1 Names nck-associated protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Canis lupus familiaris (dog)	NCKAP1	XP_535990.3
Xenopus tropicalis (tropical clawed frog)	nckap1	XP_004917966.1
Pan troglodytes (chimpanzee)	NCKAP1	XP_001148485.2
Rattus norvegicus (Norway rat)	Nckap1	NP_113806.1
Drosophila melanogaster (fruit fly)	Hem	NP_524214.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004946	XP_315044.2
	NCKAP1	XP_001099879.2
Bos taurus (cattle)	LOC101907541	XP_005202363.1
Mus musculus (house mouse)	Nckap1	NP_058661.1
Arabidopsis thaliana (thale cress)	GRL	NP_001031485.1
Oryza sativa (rice)	Os08g0544500	NP_001062406.2
Homo sapiens (human)	NCKAP1	NP_995314.1
Gallus gallus (chicken)	NCKAP1	XP_001232275.2
Danio rerio (zebrafish)	nckap1	NP_001131137.1
Caenorhabditis elegans (roundworm)	gex-3	NP_502368.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

nckap1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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