zgrf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	zgrf1
Entrez Gene ID	100327248
Full Name	zinc finger, GRF-type containing 1
General protein information	Preferred Names zinc finger, GRF-type containing 1 Names protein ZGRF1 prematurely terminated mRNA decay factor-like
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001171530.1	NP_001165001.1	protein ZGRF1
XM_031902787.1	XP_031758647.1	protein ZGRF1 isoform X1
XM_031902794.1	XP_031758654.1	protein ZGRF1 isoform X1
XM_031902801.1	XP_031758661.1	protein ZGRF1 isoform X1
XM_031902808.1	XP_031758668.1	protein ZGRF1 isoform X2
XM_031902812.1	XP_031758672.1	protein ZGRF1 isoform X3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following zgrf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zgrf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa24902	NM_001171530.1 Latest version!	Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa48129	XM_031902794.1 Latest version!	Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa48129	XM_031902801.1 Latest version!	Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa48129	XM_031902787.1 Latest version!	Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa48130	XM_031902808.1 Latest version!	Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa48131	XM_031902812.1 Latest version!	Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zgrf1 ( NM_001171530.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001165001.1 Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), mRNA.

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CloneID	OXa24902
Clone ID Related Accession (Same CDS sequence)	NM_001171530.1
Accession Version	NM_001171530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-28
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein ZGRF1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161480.1. ##Evidence-Data-START## Transcript exon combination :: BC161480.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1696417, SAMEA1696418 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCTC AGGCATTTAT CGTTTTGTAC ACTCATCAAA AGACTAAAAA GTCCAAAACA 
TGGCAAGATG GAATTTTAAA GCTATCACCA GAAGGGAGAG TGGCAACATT ATATAATGAC 
AAAGGACAAC ACCTGGAAAA TGTTTATTTG AAAACAAATA AGTTGAATTC AGGGGATGAT 
TTGGAAAGTG ACCGGTATTT AATCACAGTT GAAGGTGAAG ATTTAAGTGG TGGTATCCCA 
CAGACCTCTA CTGAAGTCAG AGAAGCTCCC AAAATCAGTA AAAGCACATT GAAACCTGTT 
GGTCTTCTTC ATCCTGTTGG CCTGAAAAGA AAATTTTCTG GTTTTCAAGG TCCACGTGAA 
ATTTCCAAGA AGCCGTCTCT GGGGGTAGAG GGTGAAACCC AATCTCCAGT GCTCGCCCAA 
GGTTGTTCCT CTTTGCCTTC ACAGTTGTAC TCTGCATCTC CACTGTTTGC TACTCCTTGC 
TTAAAGAATT CAGATGCCAT TCTGCCACCT GATTGTGGTT CTCTTGCCTG CTGGAAAAAC 
AATCCTGATG GAAGAAATCA GACTTTACCA TTGACTTCTT CTTTTTCATG CTCATATTCA 
TCTAATGATT TGTGGATGAC TGAAAGTGAA AAGGTTGACA AGTCCTCTTC TACAGCCGCC 
AATGTGGAAG TGAATAGTGT CACACAAAAG TTTAGAAGCA GATCACAAAT TTTGGCACTG 
TTAAAGTCTC AACCTCCCCA GCAAACCAGT GCAGCATTAT CTAAATCTAA TGAGCACTAT 
GATGAACTTT CTGGTTCTGG GCATAGCGAT GTGTTAAATA CGAAGGATAC AATTATGCCT 
GGAAAAGAAA ACTGTTTTGT AGTTGTTGGA CAGAAATCTA AATATCCACA AGACATACCT 
GTTGAACCTA ATCTTAAAAA GAGTCGGTGG GATGCATATT TGGAGCATTC ACCACCCTCT 
GATTCTGAAG ATACTGTTAA TAGTGACAAG GATAAATCTG TTGAACAAGA TGACCCATAT 
AAGGTGCAAT GCTTTTCAAG TTCCTTAGAC TTGACTCAGA AAACTAGTTC AAGAAAGAAC 
ATTCAGGGCA AACCACTGAG TCCACAATTA TCAGCACAGT CTGAAAATTG CTTACCACCT 
CAGTTAGCTG AAGCAACCAA ATTCCACACT CCACAGACCA GTGTATTACA TTTGCAGTGG 
AAAACAGAAA CAAGTAATTG TAAAAGTTAT GTAGGACAAG AAGACTTGAA TACAGATAAT 
TGCTCAAATG CTACTCAACC ATTTGGGGAT ATCTCCTTTT CCTCTGAATA CAGTCTTCAA 
GGATGTAGAG ATAATGAGAC ACCCATATCA GAGAAACCTG TTGCTTCCCC ATCTCTGAAG 
ATATCTGATA CAGCTATATG TTCTTCTGAA GATGAAACCA GTGACCCAGC AATGACAGAA 
ATCACATTCA ACTTGTTGGA CAGTTTTGAT TGCATAGTCT CAGATGATGA GGATTCACAA 
ATAGTTTGCC CTAATATAAA AGAAGCAGAA CATGGCCCAC TTGGTGAAAT AATACCCACT 
AAAGATCGAA AGGACAATGT GCCTTTGCAT TGCCAGAATG AAAAACATGC AGGGAATGAA 
ATTATGGAAT CCAGAGATAT AACTGACATG TCATCTGGTT CTAATATCTC AAGAACCATA 
CTGTTTTTAT GGAATGACGT GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165001.1
CDS	110..1816
Translation	MAAQAFIVLYTHQKTKKSKTWQDGILKLSPEGRVATLYNDKGQHLENVYLKTNKLNSGDDLESDRYLITVEGEDLSGGIPQTSTEVREAPKISKSTLKPVGLLHPVGLKRKFSGFQGPREISKKPSLGVEGETQSPVLAQGCSSLPSQLYSASPLFATPCLKNSDAILPPDCGSLACWKNNPDGRNQTLPLTSSFSCSYSSNDLWMTESEKVDKSSSTAANVEVNSVTQKFRSRSQILALLKSQPPQQTSAALSKSNEHYDELSGSGHSDVLNTKDTIMPGKENCFVVVGQKSKYPQDIPVEPNLKKSRWDAYLEHSPPSDSEDTVNSDKDKSVEQDDPYKVQCFSSSLDLTQKTSSRKNIQGKPLSPQLSAQSENCLPPQLAEATKFHTPQTSVLHLQWKTETSNCKSYVGQEDLNTDNCSNATQPFGDISFSSEYSLQGCRDNETPISEKPVASPSLKISDTAICSSEDETSDPAMTEITFNLLDSFDCIVSDDEDSQIVCPNIKEAEHGPLGEIIPTKDRKDNVPLHCQNEKHAGNEIMESRDITDMSSGSNISRTILFLWNDVE

Target ORF information:

RefSeq Version	NM_001171530.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171530.1

ORF Insert Sequence:

ATGGCTGCTC AGGCATTTAT CGTTTTGTAC ACTCATCAAA AGACTAAAAA GTCCAAAACA 
TGGCAAGATG GAATTTTAAA GCTATCACCA GAAGGGAGAG TGGCAACATT ATATAATGAC 
AAAGGACAAC ACCTGGAAAA TGTTTATTTG AAAACAAATA AGTTGAATTC AGGGGATGAT 
TTGGAAAGTG ACCGGTATTT AATCACAGTT GAAGGTGAAG ATTTAAGTGG TGGTATCCCA 
CAGACCTCTA CTGAAGTCAG AGAAGCTCCC AAAATCAGTA AAAGCACATT GAAACCTGTT 
GGTCTTCTTC ATCCTGTTGG CCTGAAAAGA AAATTTTCTG GTTTTCAAGG TCCACGTGAA 
ATTTCCAAGA AGCCGTCTCT GGGGGTAGAG GGTGAAACCC AATCTCCAGT GCTCGCCCAA 
GGTTGTTCCT CTTTGCCTTC ACAGTTGTAC TCTGCATCTC CACTGTTTGC TACTCCTTGC 
TTAAAGAATT CAGATGCCAT TCTGCCACCT GATTGTGGTT CTCTTGCCTG CTGGAAAAAC 
AATCCTGATG GAAGAAATCA GACTTTACCA TTGACTTCTT CTTTTTCATG CTCATATTCA 
TCTAATGATT TGTGGATGAC TGAAAGTGAA AAGGTTGACA AGTCCTCTTC TACAGCCGCC 
AATGTGGAAG TGAATAGTGT CACACAAAAG TTTAGAAGCA GATCACAAAT TTTGGCACTG 
TTAAAGTCTC AACCTCCCCA GCAAACCAGT GCAGCATTAT CTAAATCTAA TGAGCACTAT 
GATGAACTTT CTGGTTCTGG GCATAGCGAT GTGTTAAATA CGAAGGATAC AATTATGCCT 
GGAAAAGAAA ACTGTTTTGT AGTTGTTGGA CAGAAATCTA AATATCCACA AGACATACCT 
GTTGAACCTA ATCTTAAAAA GAGTCGGTGG GATGCATATT TGGAGCATTC ACCACCCTCT 
GATTCTGAAG ATACTGTTAA TAGTGACAAG GATAAATCTG TTGAACAAGA TGACCCATAT 
AAGGTGCAAT GCTTTTCAAG TTCCTTAGAC TTGACTCAGA AAACTAGTTC AAGAAAGAAC 
ATTCAGGGCA AACCACTGAG TCCACAATTA TCAGCACAGT CTGAAAATTG CTTACCACCT 
CAGTTAGCTG AAGCAACCAA ATTCCACACT CCACAGACCA GTGTATTACA TTTGCAGTGG 
AAAACAGAAA CAAGTAATTG TAAAAGTTAT GTAGGACAAG AAGACTTGAA TACAGATAAT 
TGCTCAAATG CTACTCAACC ATTTGGGGAT ATCTCCTTTT CCTCTGAATA CAGTCTTCAA 
GGATGTAGAG ATAATGAGAC ACCCATATCA GAGAAACCTG TTGCTTCCCC ATCTCTGAAG 
ATATCTGATA CAGCTATATG TTCTTCTGAA GATGAAACCA GTGACCCAGC AATGACAGAA 
ATCACATTCA ACTTGTTGGA CAGTTTTGAT TGCATAGTCT CAGATGATGA GGATTCACAA 
ATAGTTTGCC CTAATATAAA AGAAGCAGAA CATGGCCCAC TTGGTGAAAT AATACCCACT 
AAAGATCGAA AGGACAATGT GCCTTTGCAT TGCCAGAATG AAAAACATGC AGGGAATGAA 
ATTATGGAAT CCAGAGATAT AACTGACATG TCATCTGGTT CTAATATCTC AAGAACCATA 
CTGTTTTTAT GGAATGACGT GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgrf1 ( XM_031902787.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758647.1 Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa48129
Clone ID Related Accession (Same CDS sequence)	XM_031902787.1 , XM_031902794.1 , XM_031902801.1
Accession Version	XM_031902787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein ZGRF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC AGGCATTTAT CGTTTTGTAC ACTCATCAAA AGACTAAAAA GTCCAAAACA 
TGGCAAGATG GAATTTTAAA GCTATCACCA GAAGGGAGAG TGGCAACATT ATATAATGAC 
AAAGGACAAC ACCTGGAAAA TGTTTATTTG AAAACAAATA AGTTGAATTC AGGGGATGAT 
TTGGAAAGTG ACCGGTATTT AATCACAGTT GAAGGTGAAG ATTTAAGTGG TGGTATCCCA 
CAGACCTCTA CTGAAGTCAG AGAAGCTCCC AAAATCAGTA AAAGCACATT GAAACCTGTT 
GGTCTTCTTC ATCCTGTTGG CCTGAAAAGA AAATTTTCTG GTTTTCAAGG TCCACGTGAA 
ATTTCCAAGA AGCCGTCTCT GGGGGTAGAG GGTGAAACCC AATCTCCAGT GCTCGCCCAA 
GGTTGTTCCT CTTTGCCTTC ACAGTTGTAC TCTGCATCTC CACTGTTTGC TACTCCTTGC 
TTAAAGAATT CAGATGCCAT TCTGCCACCT GATTGTGGTT CTCTTGCCTG CTGGAAAAAC 
AATCCTGATG GAAGAAATCA GACTTTACCA TTGACTTCTT CTTTTTCATG CTCATATTCA 
TCTAATGATT TGTGGATGAC TGAAAGTGAA AAGGTTGACA AGTCCTCTTC TACAGCCGCC 
AATGTGGAAG TGAATAGTGT CACACAAAAG TTTAGAAGCA GATCACAAAT TTTGGCACTG 
TTAAAGTCTC AACCTCCCCA GCAAACCAGT GCAGCATTAT CTAAATCTAA TGAGCACTAT 
GATGAACTTT CTGGTTCTGG GCATAGCGAT GTGTTAAATA CGAAGGATAC AATTATGCCT 
GGAAAAGAAA ACTGTTTTGT AGTTGTTGGA CAGAAATCTA AATATCCACA AGACATACCT 
GTTGAACCTA ATCTTAAAAA GAGTCGGTGG GATGCATATT TGGAGCATTC ACCACCCTCT 
GATTCTGAAG ATACTGTTAA TAGTGACAAG GATAAATCTG TTGAACAAGA TGACCCATAT 
AAGGTGCAAT GCTTTTCAAG TTCCTTAGAC TTGACTCAGA AAACTAGTTC AAGAAAGAAC 
ATTCAGGGCA AACCACTGAG TCCACAATTA TCAGCACAGT CTGAAAATTG CTTACCACCT 
CAGTTAGCTG AAGCAACCAA ATTCCACACT CCACAGACCA GTGTATTACA TTTGCAGTGG 
AAAACAGAAA CAAGTAATTG TAAAAGTTAT GTAGGACAAG AAGACTTGAA TACAGATAAT 
TGCTCAAATG CTACTCAACC ATTTGGGGAT ATCTCCTTTT CCTCTGAATA CAGTCTTCAA 
GGATGTAGAG ATAATGAGAC ACCCATATCA GAGAAACCTG TTGCTTCCCC ATCTCTGAAG 
ATATCTGATA CAGCTATATG TTCTTCTGAA GATGAAACCA GTGACCCAGC AATGACAGAA 
ATCACATTCA ACTTGTTGGA CAGTTTTGAT TGCATAGTCT CAGATGATGA GGATTCACAA 
ATAGTTTGCC CTAATATAAA AGAAGCAGAA CATGGCCCAC TTGGTGAAAT AATACCCACT 
AAAGATCGAA AGGACAATGT GCCTTTGCAT TGCCAGAATG AAAAACATGC AGGGAATGAA 
ATTATGGAAT CCAGAGATAT AACTGACATG TCATCTGGTT CTAATATCTC AAGAACTAAA 
AATTATGGTT TAGTGGAATA TGACAGCAAG TTACAAGTGC TATCTGACAC TGTTAAACAT 
GAGAACTGTG CAATAAAAGA GGAACACAAG GAAAAGCTGC AAAACTTCCC TGGAACATCT 
GATTTGGGTC GTATGTTTGA CATTAAAACT GAACTTTCCC ATGACAAAAA GGACACCACA 
GAAAACACTG CGCATATATC AATGGGAAAT ATTGTACAGC CTAAAGGCAG CCTTTTGGAA 
AACTCTACAG TAGTAAAGGA GGCCTCAAAG CTTTCACAAG AGTCACAAAG AAGCACACAT 
AATTGTATAT TAGTGCTTCC CATAAACAAG TCACAATCTA AAATGCCACT TTTGGAAAAT 
CATATTCAAA GTTCAAATGA AGGGATTTTA TGTAACCAAA GTACCTTTGA TTCCACTGAA 
AATATTAAAA CAGAAAACAG CATCTCTGTT CTGAAAGGAC TGACAAAACA TAGTAATGCA 
ATAGAGAGCC TAGACATTTT AAATGATGAT TCAGAATCAA CAACTCGAGA AGATGAGTGT 
TCAGGACACA CAGATAAGCT GAGTGGAGAA GTAGAAGAAG ATCATCACAA GACTATGCAT 
GATGATTTTT TCCAGTGTGA CCTTGACCAA GCTAAACCTT TTCCCACTGC AGAATTCATA 
TTTTCACAAG AGTTTAAGTC TGACAATACT CCCCGTTTCC AGTGTCCTGA ACCTGTCCCA 
CCTAAGCTGC TATCTGCTAA ATCTTTAGAG GAAGAATTGT TTGTTGAGGA TTTTCCAGCC 
AAGCCTGGTA CAGTGCATGT ACCTCAAGGA ACCCATTGTA ATAATGCTGT CCCATTTAAA 
GCATTTGCTC TGCCTCAGTG GGATGGAGGC ACTCAGCAAT CAGATTGTGA TTGGGACCTT 
TCACAGTGGC CATCCAGAGA AACAATCAAG CCAGACCTTA CAATAGTACA AGAAAAGCCA 
GATATAGATC GGCCATTTTC ATGGCATGAA AATACAATTA TGCAGTTAAA AGAATTAAAG 
GACACACAAA ATGCCACCCA AGACATCAGT TGTACAAACA GCACAATTTC CCAAGTTGGG 
TCAAAGCTTC AGAGACTATT GCCAGTCGTA ACAACACCAG ACCTTGCAGA CTCTACCAGG 
CCAGTACCAG CAGGGTATGA ATACAAAAAA TGCTTTTTGG ACACAAATTG TAGCCCATCT 
CCCAATATAT ATACAACCAA GAACTTGATA GATGTCCCTG TACATAATGC TTTTGTTGAG 
AACATTGAAG AGGGCACAGG TTTCCTGAAA CCAACAGAGG ATAGAAATTC ACCAAAAAGA 
CAAAGCAAAT GGACAAAATA TCAAAACAAA TCTCTTTGCA GCAGAACAAA TGAAAATGTA 
GAAGATATGA AAGCAAATGA GGAGTTTTTT GCAAAGTCTT TGTTTGGAAA AGAACCATAT 
ATGGCAGACA AAGTGGATGA TAAGGCCTCT GTGGAATCTA TTTGTCTGAC TTTGTTAAAG 
GACACCCTGA GTAAAAAGAA TAGTGGTCTT GTGCAGCATG GGCTTCACAC CAAACGCCAC 
CCTACTATTA GTATTGCACA GCTTAATAAA GGCAAAACAA GTTTCCTTTC CGATTCATCA 
GGCAATGTAA AGATTTCCTG TGAGCTACAT TTTCCTAGTA GGGAAACTGT ACAGTCTACT 
GTGAGCATTC CCAAAAGAGA AATTTCCATA CCTATTCTGT ACCAATCGGT GACACATTAC 
AAACATGTCT TCACTGCAGC ACTCACAGAA CATTTGAACA TAATGATGTT TGAACTATCA 
CAACGGCTCT ACAAAGCTCT CTCCAAAGTT GATATGTCTT TCTACACATC ATCCAGAGCA 
GCAGGAGAGA TGGGTAAACA CAATGTTACT CCCCTGTGCC TGCACCAACA ACCAGCAAAA 
CTAGTTATGG TGAAAAAAGA GGGTCCAAAC AAGGGTCGTT TCTTTTACAC TTGTGATGCT 
CCCAAAAGTG ACCAGTGTAA ATTCTTTAAA TGGTTGAACG AAACTCAGTG TTCAAGCCCT 
GTACAAGGAC ATGCGCAACC CAAGGTTGTA ATGGGTGATA TGAAAAGTGT TGCCAGTTTT 
GTGAGAAGTC ACAAAGTAGC CCTTTACGAG GAGAGCCATC TAATGGTTAG GAAAGTATCT 
GGATTTCAAA ATAAAAGCTT TGGTAAGTTT AAGAAAATAA TTAATGCCAA CTCTGACTTT 
GCTGGTGAAT CTAAAACCAA ACTTTACCTG AAACTGAACA GAAAGGAAAA CTCTTCAGCT 
TACAGCAAAG ATGATTTGTG GGTTGTTTCA AAAACGATTG ACTTTGAACC AGTGGATACT 
TTCGTTGCTT TCAGTGTTTT CTTTGGTCCA TCTGCTAACA ATGAAATAGA GATTCAGCCT 
CTGAAAGGAT ACAGCCCATC CAACTGGCCA TCAAATACAC TTGTCCATGC AGTGTTGGTT 
TGTAATGCAA GCACAGAACT GACATCTCTG AGAAATATAC AGGAGCACCT AAACCCATCC 
ACACTGCCAA TCATGCCACA TCTATTGAAA ATGCAATCTG AACAGGAAGG AGAATCTAAA 
ACAAACACTG GAAAGTTCAA GCCACCATCA CTCACTGCAA AGATCCCCCA AAGATGTGAA 
CTTCCAAGCT GTAGTTTTAT ACTGGCAAAA TCACAGGAGA TGATTGAGCA GTTTAGATTG 
AATGAAGATC AAGCCACAGC CTTGATGCAG ATAGCACAGA TGATGACTAC TGCTGCAGGA 
TCTTATAAAG CAGTGGCTCC ACCAATAACA GTTATTCATG GTGTTTTTGG TGCTGGAAAA 
AGCTATCTGC TCTCTGTGGT TGTTCTGTTT CTAGTGCAGT TGTTTGAAAG GGGAAATCCA 
GCAGAAGGTT ATGAAGCATC TCCTTGGAAA ATTCTCATAT CTTCATCCAC CAATGTGGCA 
GTTGATAGAG TCCTCCTAGG ATTGCTTGAT CTCGGATTTA GCCAGTTTGT AAGAGTTGGA 
AGCATTAGAA AAATTGCAAA GCCCATCCTT CCTTTCAGTC TGCATGCTGG GTCTGAAAAT 
GAAAATGAGC AAATAAAAGA ACTTCTTGCA CTTCTCAAAG ATGACTTAAC CCCAGTAGAG 
AAGGCTTATG TGAGAAAAAG TATTGAACAA CATAAGTTAG GCACCAACAA AGCGTTACTT 
GGCCAGGTCC GTGTAGTTGG TGCAACCTGT GCTGCCTGTC CTTTTGCTTG TATGAGCAAT 
CTAAAATTTC CTGTTGTAGT TCTGGATGAG TGTAGCCAGA TGACAGAACC AGCATCTATG 
CTTCCAGTTG CAAGATTTCA GTGTGAAAAA CTAATTCTTG TTGGGGATCC AAAGCAGTTG 
TCTCCAACTA TTCAGGGCTC TGAGCCAGCA CATGAAAAAG GTCTAGAACA GACACTCTTC 
AGTCGGCTTT GTCTGATGGG CCATAAAGCT ATAATTTTAA GAACACAGTA TCGATGTCAT 
CCTGCTATTT GCTCCATAGC TAATGAACTG TTCTATGACA GCCATCTCAT AAATGGTGTA 
TCGGAAGAAG ACAGAAAACC TCTTCTGGAC TGGCTGCCAA CATTATGCTT TTATAATGTG 
AATGGCACAG AACAGGTTGA GGGAAACAAC AGCTTTTATA ATATGGAAGA GGCCAACTTT 
ACAGTTAAGC TTATTCAGTC TCTTATTGCA AGTGGAATTG AGGGATCTAT GATTGGTGTG 
ATCACTCTAT ACAAGTCACA GATGTACAAG ATTTTTAGCT TACTTGCCAG CAGTGCACAC 
TGTGATTCTA CAGATATAAA AGCAGTTCAG GTTTCAACGG TTGATGCATT CCAAGGAGCC 
GAGAAGGAAA TAATTATCTT GTCCTGTGTT CGGACAAGGC AAGTTGGATT CATAGATTCA 
GAAAAAAGAA TGAATGTTGG GCTGACCAGG GGAAAGAGGC ATTTATTGAT CATTGCTAAT 
CTCGCCTGCT TGAGGAAGAA CAAACTTTGG GAACACGTGA TCCATCACTG TGAAAGGCAG 
AAAAATGGTT TAAAACATGT GAGTCAGTGG GAAGAAAAAC TTAATGCAAT CCTTTTGCGC 
TACCAAGAGA AGAAAATAGA AGAAGACATG AAAAAGAAAA AATCCAAAGC AAGAGAGTGT 
AATGTAAACC CTTCTCCACA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758647.1
CDS	217..6063
Translation	MAAQAFIVLYTHQKTKKSKTWQDGILKLSPEGRVATLYNDKGQHLENVYLKTNKLNSGDDLESDRYLITVEGEDLSGGIPQTSTEVREAPKISKSTLKPVGLLHPVGLKRKFSGFQGPREISKKPSLGVEGETQSPVLAQGCSSLPSQLYSASPLFATPCLKNSDAILPPDCGSLACWKNNPDGRNQTLPLTSSFSCSYSSNDLWMTESEKVDKSSSTAANVEVNSVTQKFRSRSQILALLKSQPPQQTSAALSKSNEHYDELSGSGHSDVLNTKDTIMPGKENCFVVVGQKSKYPQDIPVEPNLKKSRWDAYLEHSPPSDSEDTVNSDKDKSVEQDDPYKVQCFSSSLDLTQKTSSRKNIQGKPLSPQLSAQSENCLPPQLAEATKFHTPQTSVLHLQWKTETSNCKSYVGQEDLNTDNCSNATQPFGDISFSSEYSLQGCRDNETPISEKPVASPSLKISDTAICSSEDETSDPAMTEITFNLLDSFDCIVSDDEDSQIVCPNIKEAEHGPLGEIIPTKDRKDNVPLHCQNEKHAGNEIMESRDITDMSSGSNISRTKNYGLVEYDSKLQVLSDTVKHENCAIKEEHKEKLQNFPGTSDLGRMFDIKTELSHDKKDTTENTAHISMGNIVQPKGSLLENSTVVKEASKLSQESQRSTHNCILVLPINKSQSKMPLLENHIQSSNEGILCNQSTFDSTENIKTENSISVLKGLTKHSNAIESLDILNDDSESTTREDECSGHTDKLSGEVEEDHHKTMHDDFFQCDLDQAKPFPTAEFIFSQEFKSDNTPRFQCPEPVPPKLLSAKSLEEELFVEDFPAKPGTVHVPQGTHCNNAVPFKAFALPQWDGGTQQSDCDWDLSQWPSRETIKPDLTIVQEKPDIDRPFSWHENTIMQLKELKDTQNATQDISCTNSTISQVGSKLQRLLPVVTTPDLADSTRPVPAGYEYKKCFLDTNCSPSPNIYTTKNLIDVPVHNAFVENIEEGTGFLKPTEDRNSPKRQSKWTKYQNKSLCSRTNENVEDMKANEEFFAKSLFGKEPYMADKVDDKASVESICLTLLKDTLSKKNSGLVQHGLHTKRHPTISIAQLNKGKTSFLSDSSGNVKISCELHFPSRETVQSTVSIPKREISIPILYQSVTHYKHVFTAALTEHLNIMMFELSQRLYKALSKVDMSFYTSSRAAGEMGKHNVTPLCLHQQPAKLVMVKKEGPNKGRFFYTCDAPKSDQCKFFKWLNETQCSSPVQGHAQPKVVMGDMKSVASFVRSHKVALYEESHLMVRKVSGFQNKSFGKFKKIINANSDFAGESKTKLYLKLNRKENSSAYSKDDLWVVSKTIDFEPVDTFVAFSVFFGPSANNEIEIQPLKGYSPSNWPSNTLVHAVLVCNASTELTSLRNIQEHLNPSTLPIMPHLLKMQSEQEGESKTNTGKFKPPSLTAKIPQRCELPSCSFILAKSQEMIEQFRLNEDQATALMQIAQMMTTAAGSYKAVAPPITVIHGVFGAGKSYLLSVVVLFLVQLFERGNPAEGYEASPWKILISSSTNVAVDRVLLGLLDLGFSQFVRVGSIRKIAKPILPFSLHAGSENENEQIKELLALLKDDLTPVEKAYVRKSIEQHKLGTNKALLGQVRVVGATCAACPFACMSNLKFPVVVLDECSQMTEPASMLPVARFQCEKLILVGDPKQLSPTIQGSEPAHEKGLEQTLFSRLCLMGHKAIILRTQYRCHPAICSIANELFYDSHLINGVSEEDRKPLLDWLPTLCFYNVNGTEQVEGNNSFYNMEEANFTVKLIQSLIASGIEGSMIGVITLYKSQMYKIFSLLASSAHCDSTDIKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVGLTRGKRHLLIIANLACLRKNKLWEHVIHHCERQKNGLKHVSQWEEKLNAILLRYQEKKIEEDMKKKKSKARECNVNPSPQS

Target ORF information:

RefSeq Version	XM_031902787.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902787.1

ORF Insert Sequence:

ATGGCTGCTC AGGCATTTAT CGTTTTGTAC ACTCATCAAA AGACTAAAAA GTCCAAAACA 
TGGCAAGATG GAATTTTAAA GCTATCACCA GAAGGGAGAG TGGCAACATT ATATAATGAC 
AAAGGACAAC ACCTGGAAAA TGTTTATTTG AAAACAAATA AGTTGAATTC AGGGGATGAT 
TTGGAAAGTG ACCGGTATTT AATCACAGTT GAAGGTGAAG ATTTAAGTGG TGGTATCCCA 
CAGACCTCTA CTGAAGTCAG AGAAGCTCCC AAAATCAGTA AAAGCACATT GAAACCTGTT 
GGTCTTCTTC ATCCTGTTGG CCTGAAAAGA AAATTTTCTG GTTTTCAAGG TCCACGTGAA 
ATTTCCAAGA AGCCGTCTCT GGGGGTAGAG GGTGAAACCC AATCTCCAGT GCTCGCCCAA 
GGTTGTTCCT CTTTGCCTTC ACAGTTGTAC TCTGCATCTC CACTGTTTGC TACTCCTTGC 
TTAAAGAATT CAGATGCCAT TCTGCCACCT GATTGTGGTT CTCTTGCCTG CTGGAAAAAC 
AATCCTGATG GAAGAAATCA GACTTTACCA TTGACTTCTT CTTTTTCATG CTCATATTCA 
TCTAATGATT TGTGGATGAC TGAAAGTGAA AAGGTTGACA AGTCCTCTTC TACAGCCGCC 
AATGTGGAAG TGAATAGTGT CACACAAAAG TTTAGAAGCA GATCACAAAT TTTGGCACTG 
TTAAAGTCTC AACCTCCCCA GCAAACCAGT GCAGCATTAT CTAAATCTAA TGAGCACTAT 
GATGAACTTT CTGGTTCTGG GCATAGCGAT GTGTTAAATA CGAAGGATAC AATTATGCCT 
GGAAAAGAAA ACTGTTTTGT AGTTGTTGGA CAGAAATCTA AATATCCACA AGACATACCT 
GTTGAACCTA ATCTTAAAAA GAGTCGGTGG GATGCATATT TGGAGCATTC ACCACCCTCT 
GATTCTGAAG ATACTGTTAA TAGTGACAAG GATAAATCTG TTGAACAAGA TGACCCATAT 
AAGGTGCAAT GCTTTTCAAG TTCCTTAGAC TTGACTCAGA AAACTAGTTC AAGAAAGAAC 
ATTCAGGGCA AACCACTGAG TCCACAATTA TCAGCACAGT CTGAAAATTG CTTACCACCT 
CAGTTAGCTG AAGCAACCAA ATTCCACACT CCACAGACCA GTGTATTACA TTTGCAGTGG 
AAAACAGAAA CAAGTAATTG TAAAAGTTAT GTAGGACAAG AAGACTTGAA TACAGATAAT 
TGCTCAAATG CTACTCAACC ATTTGGGGAT ATCTCCTTTT CCTCTGAATA CAGTCTTCAA 
GGATGTAGAG ATAATGAGAC ACCCATATCA GAGAAACCTG TTGCTTCCCC ATCTCTGAAG 
ATATCTGATA CAGCTATATG TTCTTCTGAA GATGAAACCA GTGACCCAGC AATGACAGAA 
ATCACATTCA ACTTGTTGGA CAGTTTTGAT TGCATAGTCT CAGATGATGA GGATTCACAA 
ATAGTTTGCC CTAATATAAA AGAAGCAGAA CATGGCCCAC TTGGTGAAAT AATACCCACT 
AAAGATCGAA AGGACAATGT GCCTTTGCAT TGCCAGAATG AAAAACATGC AGGGAATGAA 
ATTATGGAAT CCAGAGATAT AACTGACATG TCATCTGGTT CTAATATCTC AAGAACTAAA 
AATTATGGTT TAGTGGAATA TGACAGCAAG TTACAAGTGC TATCTGACAC TGTTAAACAT 
GAGAACTGTG CAATAAAAGA GGAACACAAG GAAAAGCTGC AAAACTTCCC TGGAACATCT 
GATTTGGGTC GTATGTTTGA CATTAAAACT GAACTTTCCC ATGACAAAAA GGACACCACA 
GAAAACACTG CGCATATATC AATGGGAAAT ATTGTACAGC CTAAAGGCAG CCTTTTGGAA 
AACTCTACAG TAGTAAAGGA GGCCTCAAAG CTTTCACAAG AGTCACAAAG AAGCACACAT 
AATTGTATAT TAGTGCTTCC CATAAACAAG TCACAATCTA AAATGCCACT TTTGGAAAAT 
CATATTCAAA GTTCAAATGA AGGGATTTTA TGTAACCAAA GTACCTTTGA TTCCACTGAA 
AATATTAAAA CAGAAAACAG CATCTCTGTT CTGAAAGGAC TGACAAAACA TAGTAATGCA 
ATAGAGAGCC TAGACATTTT AAATGATGAT TCAGAATCAA CAACTCGAGA AGATGAGTGT 
TCAGGACACA CAGATAAGCT GAGTGGAGAA GTAGAAGAAG ATCATCACAA GACTATGCAT 
GATGATTTTT TCCAGTGTGA CCTTGACCAA GCTAAACCTT TTCCCACTGC AGAATTCATA 
TTTTCACAAG AGTTTAAGTC TGACAATACT CCCCGTTTCC AGTGTCCTGA ACCTGTCCCA 
CCTAAGCTGC TATCTGCTAA ATCTTTAGAG GAAGAATTGT TTGTTGAGGA TTTTCCAGCC 
AAGCCTGGTA CAGTGCATGT ACCTCAAGGA ACCCATTGTA ATAATGCTGT CCCATTTAAA 
GCATTTGCTC TGCCTCAGTG GGATGGAGGC ACTCAGCAAT CAGATTGTGA TTGGGACCTT 
TCACAGTGGC CATCCAGAGA AACAATCAAG CCAGACCTTA CAATAGTACA AGAAAAGCCA 
GATATAGATC GGCCATTTTC ATGGCATGAA AATACAATTA TGCAGTTAAA AGAATTAAAG 
GACACACAAA ATGCCACCCA AGACATCAGT TGTACAAACA GCACAATTTC CCAAGTTGGG 
TCAAAGCTTC AGAGACTATT GCCAGTCGTA ACAACACCAG ACCTTGCAGA CTCTACCAGG 
CCAGTACCAG CAGGGTATGA ATACAAAAAA TGCTTTTTGG ACACAAATTG TAGCCCATCT 
CCCAATATAT ATACAACCAA GAACTTGATA GATGTCCCTG TACATAATGC TTTTGTTGAG 
AACATTGAAG AGGGCACAGG TTTCCTGAAA CCAACAGAGG ATAGAAATTC ACCAAAAAGA 
CAAAGCAAAT GGACAAAATA TCAAAACAAA TCTCTTTGCA GCAGAACAAA TGAAAATGTA 
GAAGATATGA AAGCAAATGA GGAGTTTTTT GCAAAGTCTT TGTTTGGAAA AGAACCATAT 
ATGGCAGACA AAGTGGATGA TAAGGCCTCT GTGGAATCTA TTTGTCTGAC TTTGTTAAAG 
GACACCCTGA GTAAAAAGAA TAGTGGTCTT GTGCAGCATG GGCTTCACAC CAAACGCCAC 
CCTACTATTA GTATTGCACA GCTTAATAAA GGCAAAACAA GTTTCCTTTC CGATTCATCA 
GGCAATGTAA AGATTTCCTG TGAGCTACAT TTTCCTAGTA GGGAAACTGT ACAGTCTACT 
GTGAGCATTC CCAAAAGAGA AATTTCCATA CCTATTCTGT ACCAATCGGT GACACATTAC 
AAACATGTCT TCACTGCAGC ACTCACAGAA CATTTGAACA TAATGATGTT TGAACTATCA 
CAACGGCTCT ACAAAGCTCT CTCCAAAGTT GATATGTCTT TCTACACATC ATCCAGAGCA 
GCAGGAGAGA TGGGTAAACA CAATGTTACT CCCCTGTGCC TGCACCAACA ACCAGCAAAA 
CTAGTTATGG TGAAAAAAGA GGGTCCAAAC AAGGGTCGTT TCTTTTACAC TTGTGATGCT 
CCCAAAAGTG ACCAGTGTAA ATTCTTTAAA TGGTTGAACG AAACTCAGTG TTCAAGCCCT 
GTACAAGGAC ATGCGCAACC CAAGGTTGTA ATGGGTGATA TGAAAAGTGT TGCCAGTTTT 
GTGAGAAGTC ACAAAGTAGC CCTTTACGAG GAGAGCCATC TAATGGTTAG GAAAGTATCT 
GGATTTCAAA ATAAAAGCTT TGGTAAGTTT AAGAAAATAA TTAATGCCAA CTCTGACTTT 
GCTGGTGAAT CTAAAACCAA ACTTTACCTG AAACTGAACA GAAAGGAAAA CTCTTCAGCT 
TACAGCAAAG ATGATTTGTG GGTTGTTTCA AAAACGATTG ACTTTGAACC AGTGGATACT 
TTCGTTGCTT TCAGTGTTTT CTTTGGTCCA TCTGCTAACA ATGAAATAGA GATTCAGCCT 
CTGAAAGGAT ACAGCCCATC CAACTGGCCA TCAAATACAC TTGTCCATGC AGTGTTGGTT 
TGTAATGCAA GCACAGAACT GACATCTCTG AGAAATATAC AGGAGCACCT AAACCCATCC 
ACACTGCCAA TCATGCCACA TCTATTGAAA ATGCAATCTG AACAGGAAGG AGAATCTAAA 
ACAAACACTG GAAAGTTCAA GCCACCATCA CTCACTGCAA AGATCCCCCA AAGATGTGAA 
CTTCCAAGCT GTAGTTTTAT ACTGGCAAAA TCACAGGAGA TGATTGAGCA GTTTAGATTG 
AATGAAGATC AAGCCACAGC CTTGATGCAG ATAGCACAGA TGATGACTAC TGCTGCAGGA 
TCTTATAAAG CAGTGGCTCC ACCAATAACA GTTATTCATG GTGTTTTTGG TGCTGGAAAA 
AGCTATCTGC TCTCTGTGGT TGTTCTGTTT CTAGTGCAGT TGTTTGAAAG GGGAAATCCA 
GCAGAAGGTT ATGAAGCATC TCCTTGGAAA ATTCTCATAT CTTCATCCAC CAATGTGGCA 
GTTGATAGAG TCCTCCTAGG ATTGCTTGAT CTCGGATTTA GCCAGTTTGT AAGAGTTGGA 
AGCATTAGAA AAATTGCAAA GCCCATCCTT CCTTTCAGTC TGCATGCTGG GTCTGAAAAT 
GAAAATGAGC AAATAAAAGA ACTTCTTGCA CTTCTCAAAG ATGACTTAAC CCCAGTAGAG 
AAGGCTTATG TGAGAAAAAG TATTGAACAA CATAAGTTAG GCACCAACAA AGCGTTACTT 
GGCCAGGTCC GTGTAGTTGG TGCAACCTGT GCTGCCTGTC CTTTTGCTTG TATGAGCAAT 
CTAAAATTTC CTGTTGTAGT TCTGGATGAG TGTAGCCAGA TGACAGAACC AGCATCTATG 
CTTCCAGTTG CAAGATTTCA GTGTGAAAAA CTAATTCTTG TTGGGGATCC AAAGCAGTTG 
TCTCCAACTA TTCAGGGCTC TGAGCCAGCA CATGAAAAAG GTCTAGAACA GACACTCTTC 
AGTCGGCTTT GTCTGATGGG CCATAAAGCT ATAATTTTAA GAACACAGTA TCGATGTCAT 
CCTGCTATTT GCTCCATAGC TAATGAACTG TTCTATGACA GCCATCTCAT AAATGGTGTA 
TCGGAAGAAG ACAGAAAACC TCTTCTGGAC TGGCTGCCAA CATTATGCTT TTATAATGTG 
AATGGCACAG AACAGGTTGA GGGAAACAAC AGCTTTTATA ATATGGAAGA GGCCAACTTT 
ACAGTTAAGC TTATTCAGTC TCTTATTGCA AGTGGAATTG AGGGATCTAT GATTGGTGTG 
ATCACTCTAT ACAAGTCACA GATGTACAAG ATTTTTAGCT TACTTGCCAG CAGTGCACAC 
TGTGATTCTA CAGATATAAA AGCAGTTCAG GTTTCAACGG TTGATGCATT CCAAGGAGCC 
GAGAAGGAAA TAATTATCTT GTCCTGTGTT CGGACAAGGC AAGTTGGATT CATAGATTCA 
GAAAAAAGAA TGAATGTTGG GCTGACCAGG GGAAAGAGGC ATTTATTGAT CATTGCTAAT 
CTCGCCTGCT TGAGGAAGAA CAAACTTTGG GAACACGTGA TCCATCACTG TGAAAGGCAG 
AAAAATGGTT TAAAACATGT GAGTCAGTGG GAAGAAAAAC TTAATGCAAT CCTTTTGCGC 
TACCAAGAGA AGAAAATAGA AGAAGACATG AAAAAGAAAA AATCCAAAGC AAGAGAGTGT 
AATGTAAACC CTTCTCCACA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgrf1 ( XM_031902794.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758654.1 Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X2, mRNA.

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CloneID	OXa48129
Clone ID Related Accession (Same CDS sequence)	XM_031902787.1 , XM_031902794.1 , XM_031902801.1
Accession Version	XM_031902794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein ZGRF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC AGGCATTTAT CGTTTTGTAC ACTCATCAAA AGACTAAAAA GTCCAAAACA 
TGGCAAGATG GAATTTTAAA GCTATCACCA GAAGGGAGAG TGGCAACATT ATATAATGAC 
AAAGGACAAC ACCTGGAAAA TGTTTATTTG AAAACAAATA AGTTGAATTC AGGGGATGAT 
TTGGAAAGTG ACCGGTATTT AATCACAGTT GAAGGTGAAG ATTTAAGTGG TGGTATCCCA 
CAGACCTCTA CTGAAGTCAG AGAAGCTCCC AAAATCAGTA AAAGCACATT GAAACCTGTT 
GGTCTTCTTC ATCCTGTTGG CCTGAAAAGA AAATTTTCTG GTTTTCAAGG TCCACGTGAA 
ATTTCCAAGA AGCCGTCTCT GGGGGTAGAG GGTGAAACCC AATCTCCAGT GCTCGCCCAA 
GGTTGTTCCT CTTTGCCTTC ACAGTTGTAC TCTGCATCTC CACTGTTTGC TACTCCTTGC 
TTAAAGAATT CAGATGCCAT TCTGCCACCT GATTGTGGTT CTCTTGCCTG CTGGAAAAAC 
AATCCTGATG GAAGAAATCA GACTTTACCA TTGACTTCTT CTTTTTCATG CTCATATTCA 
TCTAATGATT TGTGGATGAC TGAAAGTGAA AAGGTTGACA AGTCCTCTTC TACAGCCGCC 
AATGTGGAAG TGAATAGTGT CACACAAAAG TTTAGAAGCA GATCACAAAT TTTGGCACTG 
TTAAAGTCTC AACCTCCCCA GCAAACCAGT GCAGCATTAT CTAAATCTAA TGAGCACTAT 
GATGAACTTT CTGGTTCTGG GCATAGCGAT GTGTTAAATA CGAAGGATAC AATTATGCCT 
GGAAAAGAAA ACTGTTTTGT AGTTGTTGGA CAGAAATCTA AATATCCACA AGACATACCT 
GTTGAACCTA ATCTTAAAAA GAGTCGGTGG GATGCATATT TGGAGCATTC ACCACCCTCT 
GATTCTGAAG ATACTGTTAA TAGTGACAAG GATAAATCTG TTGAACAAGA TGACCCATAT 
AAGGTGCAAT GCTTTTCAAG TTCCTTAGAC TTGACTCAGA AAACTAGTTC AAGAAAGAAC 
ATTCAGGGCA AACCACTGAG TCCACAATTA TCAGCACAGT CTGAAAATTG CTTACCACCT 
CAGTTAGCTG AAGCAACCAA ATTCCACACT CCACAGACCA GTGTATTACA TTTGCAGTGG 
AAAACAGAAA CAAGTAATTG TAAAAGTTAT GTAGGACAAG AAGACTTGAA TACAGATAAT 
TGCTCAAATG CTACTCAACC ATTTGGGGAT ATCTCCTTTT CCTCTGAATA CAGTCTTCAA 
GGATGTAGAG ATAATGAGAC ACCCATATCA GAGAAACCTG TTGCTTCCCC ATCTCTGAAG 
ATATCTGATA CAGCTATATG TTCTTCTGAA GATGAAACCA GTGACCCAGC AATGACAGAA 
ATCACATTCA ACTTGTTGGA CAGTTTTGAT TGCATAGTCT CAGATGATGA GGATTCACAA 
ATAGTTTGCC CTAATATAAA AGAAGCAGAA CATGGCCCAC TTGGTGAAAT AATACCCACT 
AAAGATCGAA AGGACAATGT GCCTTTGCAT TGCCAGAATG AAAAACATGC AGGGAATGAA 
ATTATGGAAT CCAGAGATAT AACTGACATG TCATCTGGTT CTAATATCTC AAGAACTAAA 
AATTATGGTT TAGTGGAATA TGACAGCAAG TTACAAGTGC TATCTGACAC TGTTAAACAT 
GAGAACTGTG CAATAAAAGA GGAACACAAG GAAAAGCTGC AAAACTTCCC TGGAACATCT 
GATTTGGGTC GTATGTTTGA CATTAAAACT GAACTTTCCC ATGACAAAAA GGACACCACA 
GAAAACACTG CGCATATATC AATGGGAAAT ATTGTACAGC CTAAAGGCAG CCTTTTGGAA 
AACTCTACAG TAGTAAAGGA GGCCTCAAAG CTTTCACAAG AGTCACAAAG AAGCACACAT 
AATTGTATAT TAGTGCTTCC CATAAACAAG TCACAATCTA AAATGCCACT TTTGGAAAAT 
CATATTCAAA GTTCAAATGA AGGGATTTTA TGTAACCAAA GTACCTTTGA TTCCACTGAA 
AATATTAAAA CAGAAAACAG CATCTCTGTT CTGAAAGGAC TGACAAAACA TAGTAATGCA 
ATAGAGAGCC TAGACATTTT AAATGATGAT TCAGAATCAA CAACTCGAGA AGATGAGTGT 
TCAGGACACA CAGATAAGCT GAGTGGAGAA GTAGAAGAAG ATCATCACAA GACTATGCAT 
GATGATTTTT TCCAGTGTGA CCTTGACCAA GCTAAACCTT TTCCCACTGC AGAATTCATA 
TTTTCACAAG AGTTTAAGTC TGACAATACT CCCCGTTTCC AGTGTCCTGA ACCTGTCCCA 
CCTAAGCTGC TATCTGCTAA ATCTTTAGAG GAAGAATTGT TTGTTGAGGA TTTTCCAGCC 
AAGCCTGGTA CAGTGCATGT ACCTCAAGGA ACCCATTGTA ATAATGCTGT CCCATTTAAA 
GCATTTGCTC TGCCTCAGTG GGATGGAGGC ACTCAGCAAT CAGATTGTGA TTGGGACCTT 
TCACAGTGGC CATCCAGAGA AACAATCAAG CCAGACCTTA CAATAGTACA AGAAAAGCCA 
GATATAGATC GGCCATTTTC ATGGCATGAA AATACAATTA TGCAGTTAAA AGAATTAAAG 
GACACACAAA ATGCCACCCA AGACATCAGT TGTACAAACA GCACAATTTC CCAAGTTGGG 
TCAAAGCTTC AGAGACTATT GCCAGTCGTA ACAACACCAG ACCTTGCAGA CTCTACCAGG 
CCAGTACCAG CAGGGTATGA ATACAAAAAA TGCTTTTTGG ACACAAATTG TAGCCCATCT 
CCCAATATAT ATACAACCAA GAACTTGATA GATGTCCCTG TACATAATGC TTTTGTTGAG 
AACATTGAAG AGGGCACAGG TTTCCTGAAA CCAACAGAGG ATAGAAATTC ACCAAAAAGA 
CAAAGCAAAT GGACAAAATA TCAAAACAAA TCTCTTTGCA GCAGAACAAA TGAAAATGTA 
GAAGATATGA AAGCAAATGA GGAGTTTTTT GCAAAGTCTT TGTTTGGAAA AGAACCATAT 
ATGGCAGACA AAGTGGATGA TAAGGCCTCT GTGGAATCTA TTTGTCTGAC TTTGTTAAAG 
GACACCCTGA GTAAAAAGAA TAGTGGTCTT GTGCAGCATG GGCTTCACAC CAAACGCCAC 
CCTACTATTA GTATTGCACA GCTTAATAAA GGCAAAACAA GTTTCCTTTC CGATTCATCA 
GGCAATGTAA AGATTTCCTG TGAGCTACAT TTTCCTAGTA GGGAAACTGT ACAGTCTACT 
GTGAGCATTC CCAAAAGAGA AATTTCCATA CCTATTCTGT ACCAATCGGT GACACATTAC 
AAACATGTCT TCACTGCAGC ACTCACAGAA CATTTGAACA TAATGATGTT TGAACTATCA 
CAACGGCTCT ACAAAGCTCT CTCCAAAGTT GATATGTCTT TCTACACATC ATCCAGAGCA 
GCAGGAGAGA TGGGTAAACA CAATGTTACT CCCCTGTGCC TGCACCAACA ACCAGCAAAA 
CTAGTTATGG TGAAAAAAGA GGGTCCAAAC AAGGGTCGTT TCTTTTACAC TTGTGATGCT 
CCCAAAAGTG ACCAGTGTAA ATTCTTTAAA TGGTTGAACG AAACTCAGTG TTCAAGCCCT 
GTACAAGGAC ATGCGCAACC CAAGGTTGTA ATGGGTGATA TGAAAAGTGT TGCCAGTTTT 
GTGAGAAGTC ACAAAGTAGC CCTTTACGAG GAGAGCCATC TAATGGTTAG GAAAGTATCT 
GGATTTCAAA ATAAAAGCTT TGGTAAGTTT AAGAAAATAA TTAATGCCAA CTCTGACTTT 
GCTGGTGAAT CTAAAACCAA ACTTTACCTG AAACTGAACA GAAAGGAAAA CTCTTCAGCT 
TACAGCAAAG ATGATTTGTG GGTTGTTTCA AAAACGATTG ACTTTGAACC AGTGGATACT 
TTCGTTGCTT TCAGTGTTTT CTTTGGTCCA TCTGCTAACA ATGAAATAGA GATTCAGCCT 
CTGAAAGGAT ACAGCCCATC CAACTGGCCA TCAAATACAC TTGTCCATGC AGTGTTGGTT 
TGTAATGCAA GCACAGAACT GACATCTCTG AGAAATATAC AGGAGCACCT AAACCCATCC 
ACACTGCCAA TCATGCCACA TCTATTGAAA ATGCAATCTG AACAGGAAGG AGAATCTAAA 
ACAAACACTG GAAAGTTCAA GCCACCATCA CTCACTGCAA AGATCCCCCA AAGATGTGAA 
CTTCCAAGCT GTAGTTTTAT ACTGGCAAAA TCACAGGAGA TGATTGAGCA GTTTAGATTG 
AATGAAGATC AAGCCACAGC CTTGATGCAG ATAGCACAGA TGATGACTAC TGCTGCAGGA 
TCTTATAAAG CAGTGGCTCC ACCAATAACA GTTATTCATG GTGTTTTTGG TGCTGGAAAA 
AGCTATCTGC TCTCTGTGGT TGTTCTGTTT CTAGTGCAGT TGTTTGAAAG GGGAAATCCA 
GCAGAAGGTT ATGAAGCATC TCCTTGGAAA ATTCTCATAT CTTCATCCAC CAATGTGGCA 
GTTGATAGAG TCCTCCTAGG ATTGCTTGAT CTCGGATTTA GCCAGTTTGT AAGAGTTGGA 
AGCATTAGAA AAATTGCAAA GCCCATCCTT CCTTTCAGTC TGCATGCTGG GTCTGAAAAT 
GAAAATGAGC AAATAAAAGA ACTTCTTGCA CTTCTCAAAG ATGACTTAAC CCCAGTAGAG 
AAGGCTTATG TGAGAAAAAG TATTGAACAA CATAAGTTAG GCACCAACAA AGCGTTACTT 
GGCCAGGTCC GTGTAGTTGG TGCAACCTGT GCTGCCTGTC CTTTTGCTTG TATGAGCAAT 
CTAAAATTTC CTGTTGTAGT TCTGGATGAG TGTAGCCAGA TGACAGAACC AGCATCTATG 
CTTCCAGTTG CAAGATTTCA GTGTGAAAAA CTAATTCTTG TTGGGGATCC AAAGCAGTTG 
TCTCCAACTA TTCAGGGCTC TGAGCCAGCA CATGAAAAAG GTCTAGAACA GACACTCTTC 
AGTCGGCTTT GTCTGATGGG CCATAAAGCT ATAATTTTAA GAACACAGTA TCGATGTCAT 
CCTGCTATTT GCTCCATAGC TAATGAACTG TTCTATGACA GCCATCTCAT AAATGGTGTA 
TCGGAAGAAG ACAGAAAACC TCTTCTGGAC TGGCTGCCAA CATTATGCTT TTATAATGTG 
AATGGCACAG AACAGGTTGA GGGAAACAAC AGCTTTTATA ATATGGAAGA GGCCAACTTT 
ACAGTTAAGC TTATTCAGTC TCTTATTGCA AGTGGAATTG AGGGATCTAT GATTGGTGTG 
ATCACTCTAT ACAAGTCACA GATGTACAAG ATTTTTAGCT TACTTGCCAG CAGTGCACAC 
TGTGATTCTA CAGATATAAA AGCAGTTCAG GTTTCAACGG TTGATGCATT CCAAGGAGCC 
GAGAAGGAAA TAATTATCTT GTCCTGTGTT CGGACAAGGC AAGTTGGATT CATAGATTCA 
GAAAAAAGAA TGAATGTTGG GCTGACCAGG GGAAAGAGGC ATTTATTGAT CATTGCTAAT 
CTCGCCTGCT TGAGGAAGAA CAAACTTTGG GAACACGTGA TCCATCACTG TGAAAGGCAG 
AAAAATGGTT TAAAACATGT GAGTCAGTGG GAAGAAAAAC TTAATGCAAT CCTTTTGCGC 
TACCAAGAGA AGAAAATAGA AGAAGACATG AAAAAGAAAA AATCCAAAGC AAGAGAGTGT 
AATGTAAACC CTTCTCCACA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758654.1
CDS	1638..7484
Translation	MAAQAFIVLYTHQKTKKSKTWQDGILKLSPEGRVATLYNDKGQHLENVYLKTNKLNSGDDLESDRYLITVEGEDLSGGIPQTSTEVREAPKISKSTLKPVGLLHPVGLKRKFSGFQGPREISKKPSLGVEGETQSPVLAQGCSSLPSQLYSASPLFATPCLKNSDAILPPDCGSLACWKNNPDGRNQTLPLTSSFSCSYSSNDLWMTESEKVDKSSSTAANVEVNSVTQKFRSRSQILALLKSQPPQQTSAALSKSNEHYDELSGSGHSDVLNTKDTIMPGKENCFVVVGQKSKYPQDIPVEPNLKKSRWDAYLEHSPPSDSEDTVNSDKDKSVEQDDPYKVQCFSSSLDLTQKTSSRKNIQGKPLSPQLSAQSENCLPPQLAEATKFHTPQTSVLHLQWKTETSNCKSYVGQEDLNTDNCSNATQPFGDISFSSEYSLQGCRDNETPISEKPVASPSLKISDTAICSSEDETSDPAMTEITFNLLDSFDCIVSDDEDSQIVCPNIKEAEHGPLGEIIPTKDRKDNVPLHCQNEKHAGNEIMESRDITDMSSGSNISRTKNYGLVEYDSKLQVLSDTVKHENCAIKEEHKEKLQNFPGTSDLGRMFDIKTELSHDKKDTTENTAHISMGNIVQPKGSLLENSTVVKEASKLSQESQRSTHNCILVLPINKSQSKMPLLENHIQSSNEGILCNQSTFDSTENIKTENSISVLKGLTKHSNAIESLDILNDDSESTTREDECSGHTDKLSGEVEEDHHKTMHDDFFQCDLDQAKPFPTAEFIFSQEFKSDNTPRFQCPEPVPPKLLSAKSLEEELFVEDFPAKPGTVHVPQGTHCNNAVPFKAFALPQWDGGTQQSDCDWDLSQWPSRETIKPDLTIVQEKPDIDRPFSWHENTIMQLKELKDTQNATQDISCTNSTISQVGSKLQRLLPVVTTPDLADSTRPVPAGYEYKKCFLDTNCSPSPNIYTTKNLIDVPVHNAFVENIEEGTGFLKPTEDRNSPKRQSKWTKYQNKSLCSRTNENVEDMKANEEFFAKSLFGKEPYMADKVDDKASVESICLTLLKDTLSKKNSGLVQHGLHTKRHPTISIAQLNKGKTSFLSDSSGNVKISCELHFPSRETVQSTVSIPKREISIPILYQSVTHYKHVFTAALTEHLNIMMFELSQRLYKALSKVDMSFYTSSRAAGEMGKHNVTPLCLHQQPAKLVMVKKEGPNKGRFFYTCDAPKSDQCKFFKWLNETQCSSPVQGHAQPKVVMGDMKSVASFVRSHKVALYEESHLMVRKVSGFQNKSFGKFKKIINANSDFAGESKTKLYLKLNRKENSSAYSKDDLWVVSKTIDFEPVDTFVAFSVFFGPSANNEIEIQPLKGYSPSNWPSNTLVHAVLVCNASTELTSLRNIQEHLNPSTLPIMPHLLKMQSEQEGESKTNTGKFKPPSLTAKIPQRCELPSCSFILAKSQEMIEQFRLNEDQATALMQIAQMMTTAAGSYKAVAPPITVIHGVFGAGKSYLLSVVVLFLVQLFERGNPAEGYEASPWKILISSSTNVAVDRVLLGLLDLGFSQFVRVGSIRKIAKPILPFSLHAGSENENEQIKELLALLKDDLTPVEKAYVRKSIEQHKLGTNKALLGQVRVVGATCAACPFACMSNLKFPVVVLDECSQMTEPASMLPVARFQCEKLILVGDPKQLSPTIQGSEPAHEKGLEQTLFSRLCLMGHKAIILRTQYRCHPAICSIANELFYDSHLINGVSEEDRKPLLDWLPTLCFYNVNGTEQVEGNNSFYNMEEANFTVKLIQSLIASGIEGSMIGVITLYKSQMYKIFSLLASSAHCDSTDIKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVGLTRGKRHLLIIANLACLRKNKLWEHVIHHCERQKNGLKHVSQWEEKLNAILLRYQEKKIEEDMKKKKSKARECNVNPSPQS

Target ORF information:

RefSeq Version	XM_031902794.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902794.1

ORF Insert Sequence:

ATGGCTGCTC AGGCATTTAT CGTTTTGTAC ACTCATCAAA AGACTAAAAA GTCCAAAACA 
TGGCAAGATG GAATTTTAAA GCTATCACCA GAAGGGAGAG TGGCAACATT ATATAATGAC 
AAAGGACAAC ACCTGGAAAA TGTTTATTTG AAAACAAATA AGTTGAATTC AGGGGATGAT 
TTGGAAAGTG ACCGGTATTT AATCACAGTT GAAGGTGAAG ATTTAAGTGG TGGTATCCCA 
CAGACCTCTA CTGAAGTCAG AGAAGCTCCC AAAATCAGTA AAAGCACATT GAAACCTGTT 
GGTCTTCTTC ATCCTGTTGG CCTGAAAAGA AAATTTTCTG GTTTTCAAGG TCCACGTGAA 
ATTTCCAAGA AGCCGTCTCT GGGGGTAGAG GGTGAAACCC AATCTCCAGT GCTCGCCCAA 
GGTTGTTCCT CTTTGCCTTC ACAGTTGTAC TCTGCATCTC CACTGTTTGC TACTCCTTGC 
TTAAAGAATT CAGATGCCAT TCTGCCACCT GATTGTGGTT CTCTTGCCTG CTGGAAAAAC 
AATCCTGATG GAAGAAATCA GACTTTACCA TTGACTTCTT CTTTTTCATG CTCATATTCA 
TCTAATGATT TGTGGATGAC TGAAAGTGAA AAGGTTGACA AGTCCTCTTC TACAGCCGCC 
AATGTGGAAG TGAATAGTGT CACACAAAAG TTTAGAAGCA GATCACAAAT TTTGGCACTG 
TTAAAGTCTC AACCTCCCCA GCAAACCAGT GCAGCATTAT CTAAATCTAA TGAGCACTAT 
GATGAACTTT CTGGTTCTGG GCATAGCGAT GTGTTAAATA CGAAGGATAC AATTATGCCT 
GGAAAAGAAA ACTGTTTTGT AGTTGTTGGA CAGAAATCTA AATATCCACA AGACATACCT 
GTTGAACCTA ATCTTAAAAA GAGTCGGTGG GATGCATATT TGGAGCATTC ACCACCCTCT 
GATTCTGAAG ATACTGTTAA TAGTGACAAG GATAAATCTG TTGAACAAGA TGACCCATAT 
AAGGTGCAAT GCTTTTCAAG TTCCTTAGAC TTGACTCAGA AAACTAGTTC AAGAAAGAAC 
ATTCAGGGCA AACCACTGAG TCCACAATTA TCAGCACAGT CTGAAAATTG CTTACCACCT 
CAGTTAGCTG AAGCAACCAA ATTCCACACT CCACAGACCA GTGTATTACA TTTGCAGTGG 
AAAACAGAAA CAAGTAATTG TAAAAGTTAT GTAGGACAAG AAGACTTGAA TACAGATAAT 
TGCTCAAATG CTACTCAACC ATTTGGGGAT ATCTCCTTTT CCTCTGAATA CAGTCTTCAA 
GGATGTAGAG ATAATGAGAC ACCCATATCA GAGAAACCTG TTGCTTCCCC ATCTCTGAAG 
ATATCTGATA CAGCTATATG TTCTTCTGAA GATGAAACCA GTGACCCAGC AATGACAGAA 
ATCACATTCA ACTTGTTGGA CAGTTTTGAT TGCATAGTCT CAGATGATGA GGATTCACAA 
ATAGTTTGCC CTAATATAAA AGAAGCAGAA CATGGCCCAC TTGGTGAAAT AATACCCACT 
AAAGATCGAA AGGACAATGT GCCTTTGCAT TGCCAGAATG AAAAACATGC AGGGAATGAA 
ATTATGGAAT CCAGAGATAT AACTGACATG TCATCTGGTT CTAATATCTC AAGAACTAAA 
AATTATGGTT TAGTGGAATA TGACAGCAAG TTACAAGTGC TATCTGACAC TGTTAAACAT 
GAGAACTGTG CAATAAAAGA GGAACACAAG GAAAAGCTGC AAAACTTCCC TGGAACATCT 
GATTTGGGTC GTATGTTTGA CATTAAAACT GAACTTTCCC ATGACAAAAA GGACACCACA 
GAAAACACTG CGCATATATC AATGGGAAAT ATTGTACAGC CTAAAGGCAG CCTTTTGGAA 
AACTCTACAG TAGTAAAGGA GGCCTCAAAG CTTTCACAAG AGTCACAAAG AAGCACACAT 
AATTGTATAT TAGTGCTTCC CATAAACAAG TCACAATCTA AAATGCCACT TTTGGAAAAT 
CATATTCAAA GTTCAAATGA AGGGATTTTA TGTAACCAAA GTACCTTTGA TTCCACTGAA 
AATATTAAAA CAGAAAACAG CATCTCTGTT CTGAAAGGAC TGACAAAACA TAGTAATGCA 
ATAGAGAGCC TAGACATTTT AAATGATGAT TCAGAATCAA CAACTCGAGA AGATGAGTGT 
TCAGGACACA CAGATAAGCT GAGTGGAGAA GTAGAAGAAG ATCATCACAA GACTATGCAT 
GATGATTTTT TCCAGTGTGA CCTTGACCAA GCTAAACCTT TTCCCACTGC AGAATTCATA 
TTTTCACAAG AGTTTAAGTC TGACAATACT CCCCGTTTCC AGTGTCCTGA ACCTGTCCCA 
CCTAAGCTGC TATCTGCTAA ATCTTTAGAG GAAGAATTGT TTGTTGAGGA TTTTCCAGCC 
AAGCCTGGTA CAGTGCATGT ACCTCAAGGA ACCCATTGTA ATAATGCTGT CCCATTTAAA 
GCATTTGCTC TGCCTCAGTG GGATGGAGGC ACTCAGCAAT CAGATTGTGA TTGGGACCTT 
TCACAGTGGC CATCCAGAGA AACAATCAAG CCAGACCTTA CAATAGTACA AGAAAAGCCA 
GATATAGATC GGCCATTTTC ATGGCATGAA AATACAATTA TGCAGTTAAA AGAATTAAAG 
GACACACAAA ATGCCACCCA AGACATCAGT TGTACAAACA GCACAATTTC CCAAGTTGGG 
TCAAAGCTTC AGAGACTATT GCCAGTCGTA ACAACACCAG ACCTTGCAGA CTCTACCAGG 
CCAGTACCAG CAGGGTATGA ATACAAAAAA TGCTTTTTGG ACACAAATTG TAGCCCATCT 
CCCAATATAT ATACAACCAA GAACTTGATA GATGTCCCTG TACATAATGC TTTTGTTGAG 
AACATTGAAG AGGGCACAGG TTTCCTGAAA CCAACAGAGG ATAGAAATTC ACCAAAAAGA 
CAAAGCAAAT GGACAAAATA TCAAAACAAA TCTCTTTGCA GCAGAACAAA TGAAAATGTA 
GAAGATATGA AAGCAAATGA GGAGTTTTTT GCAAAGTCTT TGTTTGGAAA AGAACCATAT 
ATGGCAGACA AAGTGGATGA TAAGGCCTCT GTGGAATCTA TTTGTCTGAC TTTGTTAAAG 
GACACCCTGA GTAAAAAGAA TAGTGGTCTT GTGCAGCATG GGCTTCACAC CAAACGCCAC 
CCTACTATTA GTATTGCACA GCTTAATAAA GGCAAAACAA GTTTCCTTTC CGATTCATCA 
GGCAATGTAA AGATTTCCTG TGAGCTACAT TTTCCTAGTA GGGAAACTGT ACAGTCTACT 
GTGAGCATTC CCAAAAGAGA AATTTCCATA CCTATTCTGT ACCAATCGGT GACACATTAC 
AAACATGTCT TCACTGCAGC ACTCACAGAA CATTTGAACA TAATGATGTT TGAACTATCA 
CAACGGCTCT ACAAAGCTCT CTCCAAAGTT GATATGTCTT TCTACACATC ATCCAGAGCA 
GCAGGAGAGA TGGGTAAACA CAATGTTACT CCCCTGTGCC TGCACCAACA ACCAGCAAAA 
CTAGTTATGG TGAAAAAAGA GGGTCCAAAC AAGGGTCGTT TCTTTTACAC TTGTGATGCT 
CCCAAAAGTG ACCAGTGTAA ATTCTTTAAA TGGTTGAACG AAACTCAGTG TTCAAGCCCT 
GTACAAGGAC ATGCGCAACC CAAGGTTGTA ATGGGTGATA TGAAAAGTGT TGCCAGTTTT 
GTGAGAAGTC ACAAAGTAGC CCTTTACGAG GAGAGCCATC TAATGGTTAG GAAAGTATCT 
GGATTTCAAA ATAAAAGCTT TGGTAAGTTT AAGAAAATAA TTAATGCCAA CTCTGACTTT 
GCTGGTGAAT CTAAAACCAA ACTTTACCTG AAACTGAACA GAAAGGAAAA CTCTTCAGCT 
TACAGCAAAG ATGATTTGTG GGTTGTTTCA AAAACGATTG ACTTTGAACC AGTGGATACT 
TTCGTTGCTT TCAGTGTTTT CTTTGGTCCA TCTGCTAACA ATGAAATAGA GATTCAGCCT 
CTGAAAGGAT ACAGCCCATC CAACTGGCCA TCAAATACAC TTGTCCATGC AGTGTTGGTT 
TGTAATGCAA GCACAGAACT GACATCTCTG AGAAATATAC AGGAGCACCT AAACCCATCC 
ACACTGCCAA TCATGCCACA TCTATTGAAA ATGCAATCTG AACAGGAAGG AGAATCTAAA 
ACAAACACTG GAAAGTTCAA GCCACCATCA CTCACTGCAA AGATCCCCCA AAGATGTGAA 
CTTCCAAGCT GTAGTTTTAT ACTGGCAAAA TCACAGGAGA TGATTGAGCA GTTTAGATTG 
AATGAAGATC AAGCCACAGC CTTGATGCAG ATAGCACAGA TGATGACTAC TGCTGCAGGA 
TCTTATAAAG CAGTGGCTCC ACCAATAACA GTTATTCATG GTGTTTTTGG TGCTGGAAAA 
AGCTATCTGC TCTCTGTGGT TGTTCTGTTT CTAGTGCAGT TGTTTGAAAG GGGAAATCCA 
GCAGAAGGTT ATGAAGCATC TCCTTGGAAA ATTCTCATAT CTTCATCCAC CAATGTGGCA 
GTTGATAGAG TCCTCCTAGG ATTGCTTGAT CTCGGATTTA GCCAGTTTGT AAGAGTTGGA 
AGCATTAGAA AAATTGCAAA GCCCATCCTT CCTTTCAGTC TGCATGCTGG GTCTGAAAAT 
GAAAATGAGC AAATAAAAGA ACTTCTTGCA CTTCTCAAAG ATGACTTAAC CCCAGTAGAG 
AAGGCTTATG TGAGAAAAAG TATTGAACAA CATAAGTTAG GCACCAACAA AGCGTTACTT 
GGCCAGGTCC GTGTAGTTGG TGCAACCTGT GCTGCCTGTC CTTTTGCTTG TATGAGCAAT 
CTAAAATTTC CTGTTGTAGT TCTGGATGAG TGTAGCCAGA TGACAGAACC AGCATCTATG 
CTTCCAGTTG CAAGATTTCA GTGTGAAAAA CTAATTCTTG TTGGGGATCC AAAGCAGTTG 
TCTCCAACTA TTCAGGGCTC TGAGCCAGCA CATGAAAAAG GTCTAGAACA GACACTCTTC 
AGTCGGCTTT GTCTGATGGG CCATAAAGCT ATAATTTTAA GAACACAGTA TCGATGTCAT 
CCTGCTATTT GCTCCATAGC TAATGAACTG TTCTATGACA GCCATCTCAT AAATGGTGTA 
TCGGAAGAAG ACAGAAAACC TCTTCTGGAC TGGCTGCCAA CATTATGCTT TTATAATGTG 
AATGGCACAG AACAGGTTGA GGGAAACAAC AGCTTTTATA ATATGGAAGA GGCCAACTTT 
ACAGTTAAGC TTATTCAGTC TCTTATTGCA AGTGGAATTG AGGGATCTAT GATTGGTGTG 
ATCACTCTAT ACAAGTCACA GATGTACAAG ATTTTTAGCT TACTTGCCAG CAGTGCACAC 
TGTGATTCTA CAGATATAAA AGCAGTTCAG GTTTCAACGG TTGATGCATT CCAAGGAGCC 
GAGAAGGAAA TAATTATCTT GTCCTGTGTT CGGACAAGGC AAGTTGGATT CATAGATTCA 
GAAAAAAGAA TGAATGTTGG GCTGACCAGG GGAAAGAGGC ATTTATTGAT CATTGCTAAT 
CTCGCCTGCT TGAGGAAGAA CAAACTTTGG GAACACGTGA TCCATCACTG TGAAAGGCAG 
AAAAATGGTT TAAAACATGT GAGTCAGTGG GAAGAAAAAC TTAATGCAAT CCTTTTGCGC 
TACCAAGAGA AGAAAATAGA AGAAGACATG AAAAAGAAAA AATCCAAAGC AAGAGAGTGT 
AATGTAAACC CTTCTCCACA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgrf1 ( XM_031902801.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758661.1 Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X3, mRNA.

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CloneID	OXa48129
Clone ID Related Accession (Same CDS sequence)	XM_031902787.1 , XM_031902794.1 , XM_031902801.1
Accession Version	XM_031902801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein ZGRF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC AGGCATTTAT CGTTTTGTAC ACTCATCAAA AGACTAAAAA GTCCAAAACA 
TGGCAAGATG GAATTTTAAA GCTATCACCA GAAGGGAGAG TGGCAACATT ATATAATGAC 
AAAGGACAAC ACCTGGAAAA TGTTTATTTG AAAACAAATA AGTTGAATTC AGGGGATGAT 
TTGGAAAGTG ACCGGTATTT AATCACAGTT GAAGGTGAAG ATTTAAGTGG TGGTATCCCA 
CAGACCTCTA CTGAAGTCAG AGAAGCTCCC AAAATCAGTA AAAGCACATT GAAACCTGTT 
GGTCTTCTTC ATCCTGTTGG CCTGAAAAGA AAATTTTCTG GTTTTCAAGG TCCACGTGAA 
ATTTCCAAGA AGCCGTCTCT GGGGGTAGAG GGTGAAACCC AATCTCCAGT GCTCGCCCAA 
GGTTGTTCCT CTTTGCCTTC ACAGTTGTAC TCTGCATCTC CACTGTTTGC TACTCCTTGC 
TTAAAGAATT CAGATGCCAT TCTGCCACCT GATTGTGGTT CTCTTGCCTG CTGGAAAAAC 
AATCCTGATG GAAGAAATCA GACTTTACCA TTGACTTCTT CTTTTTCATG CTCATATTCA 
TCTAATGATT TGTGGATGAC TGAAAGTGAA AAGGTTGACA AGTCCTCTTC TACAGCCGCC 
AATGTGGAAG TGAATAGTGT CACACAAAAG TTTAGAAGCA GATCACAAAT TTTGGCACTG 
TTAAAGTCTC AACCTCCCCA GCAAACCAGT GCAGCATTAT CTAAATCTAA TGAGCACTAT 
GATGAACTTT CTGGTTCTGG GCATAGCGAT GTGTTAAATA CGAAGGATAC AATTATGCCT 
GGAAAAGAAA ACTGTTTTGT AGTTGTTGGA CAGAAATCTA AATATCCACA AGACATACCT 
GTTGAACCTA ATCTTAAAAA GAGTCGGTGG GATGCATATT TGGAGCATTC ACCACCCTCT 
GATTCTGAAG ATACTGTTAA TAGTGACAAG GATAAATCTG TTGAACAAGA TGACCCATAT 
AAGGTGCAAT GCTTTTCAAG TTCCTTAGAC TTGACTCAGA AAACTAGTTC AAGAAAGAAC 
ATTCAGGGCA AACCACTGAG TCCACAATTA TCAGCACAGT CTGAAAATTG CTTACCACCT 
CAGTTAGCTG AAGCAACCAA ATTCCACACT CCACAGACCA GTGTATTACA TTTGCAGTGG 
AAAACAGAAA CAAGTAATTG TAAAAGTTAT GTAGGACAAG AAGACTTGAA TACAGATAAT 
TGCTCAAATG CTACTCAACC ATTTGGGGAT ATCTCCTTTT CCTCTGAATA CAGTCTTCAA 
GGATGTAGAG ATAATGAGAC ACCCATATCA GAGAAACCTG TTGCTTCCCC ATCTCTGAAG 
ATATCTGATA CAGCTATATG TTCTTCTGAA GATGAAACCA GTGACCCAGC AATGACAGAA 
ATCACATTCA ACTTGTTGGA CAGTTTTGAT TGCATAGTCT CAGATGATGA GGATTCACAA 
ATAGTTTGCC CTAATATAAA AGAAGCAGAA CATGGCCCAC TTGGTGAAAT AATACCCACT 
AAAGATCGAA AGGACAATGT GCCTTTGCAT TGCCAGAATG AAAAACATGC AGGGAATGAA 
ATTATGGAAT CCAGAGATAT AACTGACATG TCATCTGGTT CTAATATCTC AAGAACTAAA 
AATTATGGTT TAGTGGAATA TGACAGCAAG TTACAAGTGC TATCTGACAC TGTTAAACAT 
GAGAACTGTG CAATAAAAGA GGAACACAAG GAAAAGCTGC AAAACTTCCC TGGAACATCT 
GATTTGGGTC GTATGTTTGA CATTAAAACT GAACTTTCCC ATGACAAAAA GGACACCACA 
GAAAACACTG CGCATATATC AATGGGAAAT ATTGTACAGC CTAAAGGCAG CCTTTTGGAA 
AACTCTACAG TAGTAAAGGA GGCCTCAAAG CTTTCACAAG AGTCACAAAG AAGCACACAT 
AATTGTATAT TAGTGCTTCC CATAAACAAG TCACAATCTA AAATGCCACT TTTGGAAAAT 
CATATTCAAA GTTCAAATGA AGGGATTTTA TGTAACCAAA GTACCTTTGA TTCCACTGAA 
AATATTAAAA CAGAAAACAG CATCTCTGTT CTGAAAGGAC TGACAAAACA TAGTAATGCA 
ATAGAGAGCC TAGACATTTT AAATGATGAT TCAGAATCAA CAACTCGAGA AGATGAGTGT 
TCAGGACACA CAGATAAGCT GAGTGGAGAA GTAGAAGAAG ATCATCACAA GACTATGCAT 
GATGATTTTT TCCAGTGTGA CCTTGACCAA GCTAAACCTT TTCCCACTGC AGAATTCATA 
TTTTCACAAG AGTTTAAGTC TGACAATACT CCCCGTTTCC AGTGTCCTGA ACCTGTCCCA 
CCTAAGCTGC TATCTGCTAA ATCTTTAGAG GAAGAATTGT TTGTTGAGGA TTTTCCAGCC 
AAGCCTGGTA CAGTGCATGT ACCTCAAGGA ACCCATTGTA ATAATGCTGT CCCATTTAAA 
GCATTTGCTC TGCCTCAGTG GGATGGAGGC ACTCAGCAAT CAGATTGTGA TTGGGACCTT 
TCACAGTGGC CATCCAGAGA AACAATCAAG CCAGACCTTA CAATAGTACA AGAAAAGCCA 
GATATAGATC GGCCATTTTC ATGGCATGAA AATACAATTA TGCAGTTAAA AGAATTAAAG 
GACACACAAA ATGCCACCCA AGACATCAGT TGTACAAACA GCACAATTTC CCAAGTTGGG 
TCAAAGCTTC AGAGACTATT GCCAGTCGTA ACAACACCAG ACCTTGCAGA CTCTACCAGG 
CCAGTACCAG CAGGGTATGA ATACAAAAAA TGCTTTTTGG ACACAAATTG TAGCCCATCT 
CCCAATATAT ATACAACCAA GAACTTGATA GATGTCCCTG TACATAATGC TTTTGTTGAG 
AACATTGAAG AGGGCACAGG TTTCCTGAAA CCAACAGAGG ATAGAAATTC ACCAAAAAGA 
CAAAGCAAAT GGACAAAATA TCAAAACAAA TCTCTTTGCA GCAGAACAAA TGAAAATGTA 
GAAGATATGA AAGCAAATGA GGAGTTTTTT GCAAAGTCTT TGTTTGGAAA AGAACCATAT 
ATGGCAGACA AAGTGGATGA TAAGGCCTCT GTGGAATCTA TTTGTCTGAC TTTGTTAAAG 
GACACCCTGA GTAAAAAGAA TAGTGGTCTT GTGCAGCATG GGCTTCACAC CAAACGCCAC 
CCTACTATTA GTATTGCACA GCTTAATAAA GGCAAAACAA GTTTCCTTTC CGATTCATCA 
GGCAATGTAA AGATTTCCTG TGAGCTACAT TTTCCTAGTA GGGAAACTGT ACAGTCTACT 
GTGAGCATTC CCAAAAGAGA AATTTCCATA CCTATTCTGT ACCAATCGGT GACACATTAC 
AAACATGTCT TCACTGCAGC ACTCACAGAA CATTTGAACA TAATGATGTT TGAACTATCA 
CAACGGCTCT ACAAAGCTCT CTCCAAAGTT GATATGTCTT TCTACACATC ATCCAGAGCA 
GCAGGAGAGA TGGGTAAACA CAATGTTACT CCCCTGTGCC TGCACCAACA ACCAGCAAAA 
CTAGTTATGG TGAAAAAAGA GGGTCCAAAC AAGGGTCGTT TCTTTTACAC TTGTGATGCT 
CCCAAAAGTG ACCAGTGTAA ATTCTTTAAA TGGTTGAACG AAACTCAGTG TTCAAGCCCT 
GTACAAGGAC ATGCGCAACC CAAGGTTGTA ATGGGTGATA TGAAAAGTGT TGCCAGTTTT 
GTGAGAAGTC ACAAAGTAGC CCTTTACGAG GAGAGCCATC TAATGGTTAG GAAAGTATCT 
GGATTTCAAA ATAAAAGCTT TGGTAAGTTT AAGAAAATAA TTAATGCCAA CTCTGACTTT 
GCTGGTGAAT CTAAAACCAA ACTTTACCTG AAACTGAACA GAAAGGAAAA CTCTTCAGCT 
TACAGCAAAG ATGATTTGTG GGTTGTTTCA AAAACGATTG ACTTTGAACC AGTGGATACT 
TTCGTTGCTT TCAGTGTTTT CTTTGGTCCA TCTGCTAACA ATGAAATAGA GATTCAGCCT 
CTGAAAGGAT ACAGCCCATC CAACTGGCCA TCAAATACAC TTGTCCATGC AGTGTTGGTT 
TGTAATGCAA GCACAGAACT GACATCTCTG AGAAATATAC AGGAGCACCT AAACCCATCC 
ACACTGCCAA TCATGCCACA TCTATTGAAA ATGCAATCTG AACAGGAAGG AGAATCTAAA 
ACAAACACTG GAAAGTTCAA GCCACCATCA CTCACTGCAA AGATCCCCCA AAGATGTGAA 
CTTCCAAGCT GTAGTTTTAT ACTGGCAAAA TCACAGGAGA TGATTGAGCA GTTTAGATTG 
AATGAAGATC AAGCCACAGC CTTGATGCAG ATAGCACAGA TGATGACTAC TGCTGCAGGA 
TCTTATAAAG CAGTGGCTCC ACCAATAACA GTTATTCATG GTGTTTTTGG TGCTGGAAAA 
AGCTATCTGC TCTCTGTGGT TGTTCTGTTT CTAGTGCAGT TGTTTGAAAG GGGAAATCCA 
GCAGAAGGTT ATGAAGCATC TCCTTGGAAA ATTCTCATAT CTTCATCCAC CAATGTGGCA 
GTTGATAGAG TCCTCCTAGG ATTGCTTGAT CTCGGATTTA GCCAGTTTGT AAGAGTTGGA 
AGCATTAGAA AAATTGCAAA GCCCATCCTT CCTTTCAGTC TGCATGCTGG GTCTGAAAAT 
GAAAATGAGC AAATAAAAGA ACTTCTTGCA CTTCTCAAAG ATGACTTAAC CCCAGTAGAG 
AAGGCTTATG TGAGAAAAAG TATTGAACAA CATAAGTTAG GCACCAACAA AGCGTTACTT 
GGCCAGGTCC GTGTAGTTGG TGCAACCTGT GCTGCCTGTC CTTTTGCTTG TATGAGCAAT 
CTAAAATTTC CTGTTGTAGT TCTGGATGAG TGTAGCCAGA TGACAGAACC AGCATCTATG 
CTTCCAGTTG CAAGATTTCA GTGTGAAAAA CTAATTCTTG TTGGGGATCC AAAGCAGTTG 
TCTCCAACTA TTCAGGGCTC TGAGCCAGCA CATGAAAAAG GTCTAGAACA GACACTCTTC 
AGTCGGCTTT GTCTGATGGG CCATAAAGCT ATAATTTTAA GAACACAGTA TCGATGTCAT 
CCTGCTATTT GCTCCATAGC TAATGAACTG TTCTATGACA GCCATCTCAT AAATGGTGTA 
TCGGAAGAAG ACAGAAAACC TCTTCTGGAC TGGCTGCCAA CATTATGCTT TTATAATGTG 
AATGGCACAG AACAGGTTGA GGGAAACAAC AGCTTTTATA ATATGGAAGA GGCCAACTTT 
ACAGTTAAGC TTATTCAGTC TCTTATTGCA AGTGGAATTG AGGGATCTAT GATTGGTGTG 
ATCACTCTAT ACAAGTCACA GATGTACAAG ATTTTTAGCT TACTTGCCAG CAGTGCACAC 
TGTGATTCTA CAGATATAAA AGCAGTTCAG GTTTCAACGG TTGATGCATT CCAAGGAGCC 
GAGAAGGAAA TAATTATCTT GTCCTGTGTT CGGACAAGGC AAGTTGGATT CATAGATTCA 
GAAAAAAGAA TGAATGTTGG GCTGACCAGG GGAAAGAGGC ATTTATTGAT CATTGCTAAT 
CTCGCCTGCT TGAGGAAGAA CAAACTTTGG GAACACGTGA TCCATCACTG TGAAAGGCAG 
AAAAATGGTT TAAAACATGT GAGTCAGTGG GAAGAAAAAC TTAATGCAAT CCTTTTGCGC 
TACCAAGAGA AGAAAATAGA AGAAGACATG AAAAAGAAAA AATCCAAAGC AAGAGAGTGT 
AATGTAAACC CTTCTCCACA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758661.1
CDS	244..6090
Translation	MAAQAFIVLYTHQKTKKSKTWQDGILKLSPEGRVATLYNDKGQHLENVYLKTNKLNSGDDLESDRYLITVEGEDLSGGIPQTSTEVREAPKISKSTLKPVGLLHPVGLKRKFSGFQGPREISKKPSLGVEGETQSPVLAQGCSSLPSQLYSASPLFATPCLKNSDAILPPDCGSLACWKNNPDGRNQTLPLTSSFSCSYSSNDLWMTESEKVDKSSSTAANVEVNSVTQKFRSRSQILALLKSQPPQQTSAALSKSNEHYDELSGSGHSDVLNTKDTIMPGKENCFVVVGQKSKYPQDIPVEPNLKKSRWDAYLEHSPPSDSEDTVNSDKDKSVEQDDPYKVQCFSSSLDLTQKTSSRKNIQGKPLSPQLSAQSENCLPPQLAEATKFHTPQTSVLHLQWKTETSNCKSYVGQEDLNTDNCSNATQPFGDISFSSEYSLQGCRDNETPISEKPVASPSLKISDTAICSSEDETSDPAMTEITFNLLDSFDCIVSDDEDSQIVCPNIKEAEHGPLGEIIPTKDRKDNVPLHCQNEKHAGNEIMESRDITDMSSGSNISRTKNYGLVEYDSKLQVLSDTVKHENCAIKEEHKEKLQNFPGTSDLGRMFDIKTELSHDKKDTTENTAHISMGNIVQPKGSLLENSTVVKEASKLSQESQRSTHNCILVLPINKSQSKMPLLENHIQSSNEGILCNQSTFDSTENIKTENSISVLKGLTKHSNAIESLDILNDDSESTTREDECSGHTDKLSGEVEEDHHKTMHDDFFQCDLDQAKPFPTAEFIFSQEFKSDNTPRFQCPEPVPPKLLSAKSLEEELFVEDFPAKPGTVHVPQGTHCNNAVPFKAFALPQWDGGTQQSDCDWDLSQWPSRETIKPDLTIVQEKPDIDRPFSWHENTIMQLKELKDTQNATQDISCTNSTISQVGSKLQRLLPVVTTPDLADSTRPVPAGYEYKKCFLDTNCSPSPNIYTTKNLIDVPVHNAFVENIEEGTGFLKPTEDRNSPKRQSKWTKYQNKSLCSRTNENVEDMKANEEFFAKSLFGKEPYMADKVDDKASVESICLTLLKDTLSKKNSGLVQHGLHTKRHPTISIAQLNKGKTSFLSDSSGNVKISCELHFPSRETVQSTVSIPKREISIPILYQSVTHYKHVFTAALTEHLNIMMFELSQRLYKALSKVDMSFYTSSRAAGEMGKHNVTPLCLHQQPAKLVMVKKEGPNKGRFFYTCDAPKSDQCKFFKWLNETQCSSPVQGHAQPKVVMGDMKSVASFVRSHKVALYEESHLMVRKVSGFQNKSFGKFKKIINANSDFAGESKTKLYLKLNRKENSSAYSKDDLWVVSKTIDFEPVDTFVAFSVFFGPSANNEIEIQPLKGYSPSNWPSNTLVHAVLVCNASTELTSLRNIQEHLNPSTLPIMPHLLKMQSEQEGESKTNTGKFKPPSLTAKIPQRCELPSCSFILAKSQEMIEQFRLNEDQATALMQIAQMMTTAAGSYKAVAPPITVIHGVFGAGKSYLLSVVVLFLVQLFERGNPAEGYEASPWKILISSSTNVAVDRVLLGLLDLGFSQFVRVGSIRKIAKPILPFSLHAGSENENEQIKELLALLKDDLTPVEKAYVRKSIEQHKLGTNKALLGQVRVVGATCAACPFACMSNLKFPVVVLDECSQMTEPASMLPVARFQCEKLILVGDPKQLSPTIQGSEPAHEKGLEQTLFSRLCLMGHKAIILRTQYRCHPAICSIANELFYDSHLINGVSEEDRKPLLDWLPTLCFYNVNGTEQVEGNNSFYNMEEANFTVKLIQSLIASGIEGSMIGVITLYKSQMYKIFSLLASSAHCDSTDIKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVGLTRGKRHLLIIANLACLRKNKLWEHVIHHCERQKNGLKHVSQWEEKLNAILLRYQEKKIEEDMKKKKSKARECNVNPSPQS

Target ORF information:

RefSeq Version	XM_031902801.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902801.1

ORF Insert Sequence:

ATGGCTGCTC AGGCATTTAT CGTTTTGTAC ACTCATCAAA AGACTAAAAA GTCCAAAACA 
TGGCAAGATG GAATTTTAAA GCTATCACCA GAAGGGAGAG TGGCAACATT ATATAATGAC 
AAAGGACAAC ACCTGGAAAA TGTTTATTTG AAAACAAATA AGTTGAATTC AGGGGATGAT 
TTGGAAAGTG ACCGGTATTT AATCACAGTT GAAGGTGAAG ATTTAAGTGG TGGTATCCCA 
CAGACCTCTA CTGAAGTCAG AGAAGCTCCC AAAATCAGTA AAAGCACATT GAAACCTGTT 
GGTCTTCTTC ATCCTGTTGG CCTGAAAAGA AAATTTTCTG GTTTTCAAGG TCCACGTGAA 
ATTTCCAAGA AGCCGTCTCT GGGGGTAGAG GGTGAAACCC AATCTCCAGT GCTCGCCCAA 
GGTTGTTCCT CTTTGCCTTC ACAGTTGTAC TCTGCATCTC CACTGTTTGC TACTCCTTGC 
TTAAAGAATT CAGATGCCAT TCTGCCACCT GATTGTGGTT CTCTTGCCTG CTGGAAAAAC 
AATCCTGATG GAAGAAATCA GACTTTACCA TTGACTTCTT CTTTTTCATG CTCATATTCA 
TCTAATGATT TGTGGATGAC TGAAAGTGAA AAGGTTGACA AGTCCTCTTC TACAGCCGCC 
AATGTGGAAG TGAATAGTGT CACACAAAAG TTTAGAAGCA GATCACAAAT TTTGGCACTG 
TTAAAGTCTC AACCTCCCCA GCAAACCAGT GCAGCATTAT CTAAATCTAA TGAGCACTAT 
GATGAACTTT CTGGTTCTGG GCATAGCGAT GTGTTAAATA CGAAGGATAC AATTATGCCT 
GGAAAAGAAA ACTGTTTTGT AGTTGTTGGA CAGAAATCTA AATATCCACA AGACATACCT 
GTTGAACCTA ATCTTAAAAA GAGTCGGTGG GATGCATATT TGGAGCATTC ACCACCCTCT 
GATTCTGAAG ATACTGTTAA TAGTGACAAG GATAAATCTG TTGAACAAGA TGACCCATAT 
AAGGTGCAAT GCTTTTCAAG TTCCTTAGAC TTGACTCAGA AAACTAGTTC AAGAAAGAAC 
ATTCAGGGCA AACCACTGAG TCCACAATTA TCAGCACAGT CTGAAAATTG CTTACCACCT 
CAGTTAGCTG AAGCAACCAA ATTCCACACT CCACAGACCA GTGTATTACA TTTGCAGTGG 
AAAACAGAAA CAAGTAATTG TAAAAGTTAT GTAGGACAAG AAGACTTGAA TACAGATAAT 
TGCTCAAATG CTACTCAACC ATTTGGGGAT ATCTCCTTTT CCTCTGAATA CAGTCTTCAA 
GGATGTAGAG ATAATGAGAC ACCCATATCA GAGAAACCTG TTGCTTCCCC ATCTCTGAAG 
ATATCTGATA CAGCTATATG TTCTTCTGAA GATGAAACCA GTGACCCAGC AATGACAGAA 
ATCACATTCA ACTTGTTGGA CAGTTTTGAT TGCATAGTCT CAGATGATGA GGATTCACAA 
ATAGTTTGCC CTAATATAAA AGAAGCAGAA CATGGCCCAC TTGGTGAAAT AATACCCACT 
AAAGATCGAA AGGACAATGT GCCTTTGCAT TGCCAGAATG AAAAACATGC AGGGAATGAA 
ATTATGGAAT CCAGAGATAT AACTGACATG TCATCTGGTT CTAATATCTC AAGAACTAAA 
AATTATGGTT TAGTGGAATA TGACAGCAAG TTACAAGTGC TATCTGACAC TGTTAAACAT 
GAGAACTGTG CAATAAAAGA GGAACACAAG GAAAAGCTGC AAAACTTCCC TGGAACATCT 
GATTTGGGTC GTATGTTTGA CATTAAAACT GAACTTTCCC ATGACAAAAA GGACACCACA 
GAAAACACTG CGCATATATC AATGGGAAAT ATTGTACAGC CTAAAGGCAG CCTTTTGGAA 
AACTCTACAG TAGTAAAGGA GGCCTCAAAG CTTTCACAAG AGTCACAAAG AAGCACACAT 
AATTGTATAT TAGTGCTTCC CATAAACAAG TCACAATCTA AAATGCCACT TTTGGAAAAT 
CATATTCAAA GTTCAAATGA AGGGATTTTA TGTAACCAAA GTACCTTTGA TTCCACTGAA 
AATATTAAAA CAGAAAACAG CATCTCTGTT CTGAAAGGAC TGACAAAACA TAGTAATGCA 
ATAGAGAGCC TAGACATTTT AAATGATGAT TCAGAATCAA CAACTCGAGA AGATGAGTGT 
TCAGGACACA CAGATAAGCT GAGTGGAGAA GTAGAAGAAG ATCATCACAA GACTATGCAT 
GATGATTTTT TCCAGTGTGA CCTTGACCAA GCTAAACCTT TTCCCACTGC AGAATTCATA 
TTTTCACAAG AGTTTAAGTC TGACAATACT CCCCGTTTCC AGTGTCCTGA ACCTGTCCCA 
CCTAAGCTGC TATCTGCTAA ATCTTTAGAG GAAGAATTGT TTGTTGAGGA TTTTCCAGCC 
AAGCCTGGTA CAGTGCATGT ACCTCAAGGA ACCCATTGTA ATAATGCTGT CCCATTTAAA 
GCATTTGCTC TGCCTCAGTG GGATGGAGGC ACTCAGCAAT CAGATTGTGA TTGGGACCTT 
TCACAGTGGC CATCCAGAGA AACAATCAAG CCAGACCTTA CAATAGTACA AGAAAAGCCA 
GATATAGATC GGCCATTTTC ATGGCATGAA AATACAATTA TGCAGTTAAA AGAATTAAAG 
GACACACAAA ATGCCACCCA AGACATCAGT TGTACAAACA GCACAATTTC CCAAGTTGGG 
TCAAAGCTTC AGAGACTATT GCCAGTCGTA ACAACACCAG ACCTTGCAGA CTCTACCAGG 
CCAGTACCAG CAGGGTATGA ATACAAAAAA TGCTTTTTGG ACACAAATTG TAGCCCATCT 
CCCAATATAT ATACAACCAA GAACTTGATA GATGTCCCTG TACATAATGC TTTTGTTGAG 
AACATTGAAG AGGGCACAGG TTTCCTGAAA CCAACAGAGG ATAGAAATTC ACCAAAAAGA 
CAAAGCAAAT GGACAAAATA TCAAAACAAA TCTCTTTGCA GCAGAACAAA TGAAAATGTA 
GAAGATATGA AAGCAAATGA GGAGTTTTTT GCAAAGTCTT TGTTTGGAAA AGAACCATAT 
ATGGCAGACA AAGTGGATGA TAAGGCCTCT GTGGAATCTA TTTGTCTGAC TTTGTTAAAG 
GACACCCTGA GTAAAAAGAA TAGTGGTCTT GTGCAGCATG GGCTTCACAC CAAACGCCAC 
CCTACTATTA GTATTGCACA GCTTAATAAA GGCAAAACAA GTTTCCTTTC CGATTCATCA 
GGCAATGTAA AGATTTCCTG TGAGCTACAT TTTCCTAGTA GGGAAACTGT ACAGTCTACT 
GTGAGCATTC CCAAAAGAGA AATTTCCATA CCTATTCTGT ACCAATCGGT GACACATTAC 
AAACATGTCT TCACTGCAGC ACTCACAGAA CATTTGAACA TAATGATGTT TGAACTATCA 
CAACGGCTCT ACAAAGCTCT CTCCAAAGTT GATATGTCTT TCTACACATC ATCCAGAGCA 
GCAGGAGAGA TGGGTAAACA CAATGTTACT CCCCTGTGCC TGCACCAACA ACCAGCAAAA 
CTAGTTATGG TGAAAAAAGA GGGTCCAAAC AAGGGTCGTT TCTTTTACAC TTGTGATGCT 
CCCAAAAGTG ACCAGTGTAA ATTCTTTAAA TGGTTGAACG AAACTCAGTG TTCAAGCCCT 
GTACAAGGAC ATGCGCAACC CAAGGTTGTA ATGGGTGATA TGAAAAGTGT TGCCAGTTTT 
GTGAGAAGTC ACAAAGTAGC CCTTTACGAG GAGAGCCATC TAATGGTTAG GAAAGTATCT 
GGATTTCAAA ATAAAAGCTT TGGTAAGTTT AAGAAAATAA TTAATGCCAA CTCTGACTTT 
GCTGGTGAAT CTAAAACCAA ACTTTACCTG AAACTGAACA GAAAGGAAAA CTCTTCAGCT 
TACAGCAAAG ATGATTTGTG GGTTGTTTCA AAAACGATTG ACTTTGAACC AGTGGATACT 
TTCGTTGCTT TCAGTGTTTT CTTTGGTCCA TCTGCTAACA ATGAAATAGA GATTCAGCCT 
CTGAAAGGAT ACAGCCCATC CAACTGGCCA TCAAATACAC TTGTCCATGC AGTGTTGGTT 
TGTAATGCAA GCACAGAACT GACATCTCTG AGAAATATAC AGGAGCACCT AAACCCATCC 
ACACTGCCAA TCATGCCACA TCTATTGAAA ATGCAATCTG AACAGGAAGG AGAATCTAAA 
ACAAACACTG GAAAGTTCAA GCCACCATCA CTCACTGCAA AGATCCCCCA AAGATGTGAA 
CTTCCAAGCT GTAGTTTTAT ACTGGCAAAA TCACAGGAGA TGATTGAGCA GTTTAGATTG 
AATGAAGATC AAGCCACAGC CTTGATGCAG ATAGCACAGA TGATGACTAC TGCTGCAGGA 
TCTTATAAAG CAGTGGCTCC ACCAATAACA GTTATTCATG GTGTTTTTGG TGCTGGAAAA 
AGCTATCTGC TCTCTGTGGT TGTTCTGTTT CTAGTGCAGT TGTTTGAAAG GGGAAATCCA 
GCAGAAGGTT ATGAAGCATC TCCTTGGAAA ATTCTCATAT CTTCATCCAC CAATGTGGCA 
GTTGATAGAG TCCTCCTAGG ATTGCTTGAT CTCGGATTTA GCCAGTTTGT AAGAGTTGGA 
AGCATTAGAA AAATTGCAAA GCCCATCCTT CCTTTCAGTC TGCATGCTGG GTCTGAAAAT 
GAAAATGAGC AAATAAAAGA ACTTCTTGCA CTTCTCAAAG ATGACTTAAC CCCAGTAGAG 
AAGGCTTATG TGAGAAAAAG TATTGAACAA CATAAGTTAG GCACCAACAA AGCGTTACTT 
GGCCAGGTCC GTGTAGTTGG TGCAACCTGT GCTGCCTGTC CTTTTGCTTG TATGAGCAAT 
CTAAAATTTC CTGTTGTAGT TCTGGATGAG TGTAGCCAGA TGACAGAACC AGCATCTATG 
CTTCCAGTTG CAAGATTTCA GTGTGAAAAA CTAATTCTTG TTGGGGATCC AAAGCAGTTG 
TCTCCAACTA TTCAGGGCTC TGAGCCAGCA CATGAAAAAG GTCTAGAACA GACACTCTTC 
AGTCGGCTTT GTCTGATGGG CCATAAAGCT ATAATTTTAA GAACACAGTA TCGATGTCAT 
CCTGCTATTT GCTCCATAGC TAATGAACTG TTCTATGACA GCCATCTCAT AAATGGTGTA 
TCGGAAGAAG ACAGAAAACC TCTTCTGGAC TGGCTGCCAA CATTATGCTT TTATAATGTG 
AATGGCACAG AACAGGTTGA GGGAAACAAC AGCTTTTATA ATATGGAAGA GGCCAACTTT 
ACAGTTAAGC TTATTCAGTC TCTTATTGCA AGTGGAATTG AGGGATCTAT GATTGGTGTG 
ATCACTCTAT ACAAGTCACA GATGTACAAG ATTTTTAGCT TACTTGCCAG CAGTGCACAC 
TGTGATTCTA CAGATATAAA AGCAGTTCAG GTTTCAACGG TTGATGCATT CCAAGGAGCC 
GAGAAGGAAA TAATTATCTT GTCCTGTGTT CGGACAAGGC AAGTTGGATT CATAGATTCA 
GAAAAAAGAA TGAATGTTGG GCTGACCAGG GGAAAGAGGC ATTTATTGAT CATTGCTAAT 
CTCGCCTGCT TGAGGAAGAA CAAACTTTGG GAACACGTGA TCCATCACTG TGAAAGGCAG 
AAAAATGGTT TAAAACATGT GAGTCAGTGG GAAGAAAAAC TTAATGCAAT CCTTTTGCGC 
TACCAAGAGA AGAAAATAGA AGAAGACATG AAAAAGAAAA AATCCAAAGC AAGAGAGTGT 
AATGTAAACC CTTCTCCACA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgrf1 ( XM_031902808.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758668.1 Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X4, mRNA.

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CloneID	OXa48130
Clone ID Related Accession (Same CDS sequence)	XM_031902808.1
Accession Version	XM_031902808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein ZGRF1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC AGGCATTTAT CGTTTTGTAC ACTCATCAAA AGACTAAAAA GTCCAAAACA 
TGGCAAGATG GAATTTTAAA GCTATCACCA GAAGGGAGAG TGGCAACATT ATATAATGAC 
AAAGGACAAC ACCTGGAAAA TGTTTATTTG AAAACAAATA AGTTGAATTC AGGGGATGAT 
TTGGAAAGTG ACCGGTATTT AATCACAGTT GAAGGTGAAG ATTTAAGTGG TGGTATCCCA 
CAGACCTCTA CTGAAGTCAG AGAAGCTCCC AAAATCAGTA AAAGCACATT GAAACCTGTT 
GGTCTTCTTC ATCCTGTTGG CCTGAAAAGA AAATTTTCTG GTTTTCAAGG TCCACGTGAA 
ATTTCCAAGA AGCCGTCTCT GGGGGTAGAG GGTGAAACCC AATCTCCAGT GCTCGCCCAA 
GGTTGTTCCT CTTTGCCTTC ACAGTTGTAC TCTGCATCTC CACTGTTTGC TACTCCTTGC 
TTAAAGAATT CAGATGCCAT TCTGCCACCT GATTGTGGTT CTCTTGCCTG CTGGAAAAAC 
AATCCTGATG GAAGAAATCA GACTTTACCA TTGACTTCTT CTTTTTCATG CTCATATTCA 
TCTAATGATT TGTGGATGAC TGAAAGTGAA AAGGTTGACA AGTCCTCTTC TACAGCCGCC 
AATGTGGAAG TGAATAGTGT CACACAAAAG TTTAGAAGCA GATCACAAAT TTTGGCACTG 
TTAAAGTCTC AACCTCCCCA GCAAACCAGT GCAGCATTAT CTAAATCTAA TGAGCACTAT 
GATGAACTTT CTGGTTCTGG GCATAGCGAT GTGTTAAATA CGAAGGATAC AATTATGCCT 
GGAAAAGAAA ACTGTTTTGT AGTTGTTGGA CAGAAATCTA AATATCCACA AGACATACCT 
GTTGAACCTA ATCTTAAAAA GAGTCGGTGG GATGCATATT TGGAGCATTC ACCACCCTCT 
GATTCTGAAG ATACTGTTAA TAGTGACAAG GATAAATCTG TTGAACAAGA TGACCCATAT 
AAGGTGCAAT GCTTTTCAAG TTCCTTAGAC TTGACTCAGA AAACTAGTTC AAGAAAGAAC 
ATTCAGGGCA AACCACTGAG TCCACAATTA TCAGCACAGT CTGAAAATTG CTTACCACCT 
CAGTTAGCTG AAGCAACCAA ATTCCACACT CCACAGACCA GTGTATTACA TTTGCAGTGG 
AAAACAGAAA CAAGTAATTG TAAAAGTTAT GTAGGACAAG AAGACTTGAA TACAGATAAT 
TGCTCAAATG CTACTCAACC ATTTGGGGAT ATCTCCTTTT CCTCTGAATA CAGTCTTCAA 
GGATGTAGAG ATAATGAGAC ACCCATATCA GAGAAACCTG TTGCTTCCCC ATCTCTGAAG 
ATATCTGATA CAGCTATATG TTCTTCTGAA GATGAAACCA GTGACCCAGC AATGACAGAA 
ATCACATTCA ACTTGTTGGA CAGTTTTGAT TGCATAGTCT CAGATGATGA GGATTCACAA 
ATAGTTTGCC CTAATATAAA AGAAGCAGAA CATGGCCCAC TTGGTGAAAT AATACCCACT 
AAAGATCGAA AGGACAATGT GCCTTTGCAT TGCCAGAATG AAAAACATGC AGGGAATGAA 
ATTATGGAAT CCAGAGATAT AACTGACATG TCATCTGGTT CTAATATCTC AAGAACTAAA 
AATTATGGTT TAGTGGAATA TGACAGCAAG TTACAAGTGC TATCTGACAC TGTTAAACAT 
GAGAACTGTG CAATAAAAGA GGAACACAAG GAAAAGCTGC AAAACTTCCC TGGAACATCT 
GATTTGGGTC GTATGTTTGA CATTAAAACT GAACTTTCCC ATGACAAAAA GGACACCACA 
GAAAACACTG CGCATATATC AATGGGAAAT ATTGTACAGC CTAAAGGCAG CCTTTTGGAA 
AACTCTACAG TAGTAAAGGA GGCCTCAAAG CTTTCACAAG AGTCACAAAG AAGCACACAT 
AATTGTATAT TAGTGCTTCC CATAAACAAG TCACAATCTA AAATGCCACT TTTGGAAAAT 
CATATTCAAA GTTCAAATGA AGGGATTTTA TGTAACCAAA GTACCTTTGA TTCCACTGAA 
AATATTAAAA CAGAAAACAG CATCTCTGTT CTGAAAGGAC TGACAAAACA TAGTAATGCA 
ATAGAGAGCC TAGACATTTT AAATGATGAT TCAGAATCAA CAACTCGAGA AGATGAGTGT 
TCAGGACACA CAGATAAGCT GAGTGGAGAA GTAGAAGAAG ATCATCACAA GACTATGCAT 
GATGATTTTT TCCAGTGTGA CCTTGACCAA GCTAAACCTT TTCCCACTGC AGAATTCATA 
TTTTCACAAG AGTTTAAGTC TGACAATACT CCCCGTTTCC AGTGTCCTGA ACCTGTCCCA 
CCTAAGCTGC TATCTGCTAA ATCTTTAGAG GAAGAATTGT TTGTTGAGGA TTTTCCAGCC 
AAGCCTGGTA CAGTGCATGT ACCTCAAGGA ACCCATTGTA ATAATGCTGT CCCATTTAAA 
GCATTTGCTC TGCCTCAGTG GGATGGAGGC ACTCAGCAAT CAGATTGTGA TTGGGACCTT 
TCACAGTGGC CATCCAGAGA AACAATCAAG CCAGACCTTA CAATAGTACA AGAAAAGCCA 
GATATAGATC GGCCATTTTC ATGGCATGAA AATACAATTA TGCAGTTAAA AGAATTAAAG 
GACACACAAA ATGCCACCCA AGACATCAGT TGTACAAACA GCACAATTTC CCAAGTTGGG 
TCAAAGCTTC AGAGACTATT GCCAGTCGTA ACAACACCAG ACCTTGCAGA CTCTACCAGG 
CCAGTACCAG CAGGGTATGA ATACAAAAAA TGCTTTTTGG ACACAAATTG TAGCCCATCT 
CCCAATATAT ATACAACCAA GAACTTGATA GATGTCCCTG TACATAATGC TTTTGTTGAG 
AACATTGAAG AGGGCACAGG TTTCCTGAAA CCAACAGAGG ATAGAAATTC ACCAAAAAGA 
CAAAGCAAAT GGACAAAATA TCAAAACAAA TCTCTTTGCA GCAGAACAAA TGAAAATGTA 
GAAGATATGA AAGCAAATGA GGAGTTTTTT GCAAAGTCTT TGTTTGGAAA AGAACCATAT 
ATGGCAGACA AAGTGGATGA TAAGGCCTCT GTGGAATCTA TTTGTCTGAC TTTGTTAAAG 
GACACCCTGA GTAAAAAGAA TAGTGGTCTT GTGCAGCATG GGCTTCACAC CAAACGCCAC 
CCTACTATTA GTATTGCACA GCTTAATAAA GGCAAAACAA GTTTCCTTTC CGATTCATCA 
GGCAATGTAA AGATTTCCTG TGAGCTACAT TTTCCTAGTA GGGAAACTGT ACAGTCTACT 
GTGAGCATTC CCAAAAGAGA AATTTCCATA CCTATTCTGT ACCAATCGGT GACACATTAC 
AAACATGTCT TCACTGCAGC ACTCACAGAA CATTTGAACA TAATGATGTT TGAACTATCA 
CAACGGCTCT ACAAAGCTCT CTCCAAAGTT GATATGTCTT TCTACACATC ATCCAGAGCA 
GCAGGAGAGA TGGGTAAACA CAATGTTACT CCCCTGTGCC TGCACCAACA ACCAGCAAAA 
CTAGTTATGG TGAAAAAAGA GGGTCCAAAC AAGGGTCGTT TCTTTTACAC TTGTGATGCT 
CCCAAAAGTG ACCAGTGTAA ATTCTTTAAA TGGTTGAACG AAACTCAGTG TTCAAGCCCT 
GTACAAGGAC ATGCGCAACC CAAGGTTGTA ATGGGTGATA TGAAAAGTGT TGCCAGTTTT 
GTGAGAAGTC ACAAAGTAGC CCTTTACGAG GAGAGCCATC TAATGGTTAG GAAAGTATCT 
GGATTTCAAA ATAAAAGCTT TGGTAAGTTT AAGAAAATAA TTAATGCCAA CTCTGACTTT 
GCTGGTGAAT CTAAAACCAA ACTTTACCTG AAACTGAACA GAAAGGAAAA CTCTTCAGCT 
TACAGCAAAG ATGATTTGTG GGTTGTTTCA AAAACGATTG ACTTTGAACC AGTGGATACT 
TTCGTTGCTT TCAGTGTTTT CTTTGGTCCA TCTGCTAACA ATGAAATAGA GATTCAGCCT 
CTGAAAGGAT ACAGCCCATC CAACTGGCCA TCAAATACAC TTGTCCATGC AGTGTTGGTT 
TGTAATGCAA GCACAGAACT GACATCTCTG AGAAATATAC AGGAGCACCT AAACCCATCC 
ACACTGCCAA TCATGCCACA TCTATTGAAA ATGCAATCTG AACAGGAAGG AGAATCTAAA 
ACAAACACTG GAAAGTTCAA GCCACCATCA CTCACTGCAA AGATCCCCCA AAGATGTGAA 
CTTCCAAGCT GTAGTTTTAT ACTGGCAAAA TCACAGGAGA TGATTGAGCA GTTTAGATTG 
AATGAAGATC AAGCCACAGC CTTGATGCAG ATAGCACAGA TGATGACTAC TGCTGCAGGA 
TCTTATAAAG CAGTGGCTCC ACCAATAACA GTTATTCATG GTGTTTTTGG TGCTGGAAAA 
AGCTATCTGC TCTCTGTGGT TGTTCTGTTT CTAGTGCAGT TGTTTGAAAG GGGAAATCCA 
GCAGAAGGTT ATGAAGCATC TCCTTGGAAA ATTCTCATAT CTTCATCCAC CAATGTGGCA 
GTTGATAGAG TCCTCCTAGG ATTGCTTGAT CTCGGATTTA GCCAGTTTGT AAGAGTTGGA 
AGCATTAGAA AAATTGCAAA GCCCATCCTT CCTTTCAGTC TGCATGCTGG GTCTGAAAAT 
GAAAATGAGC AAATAAAAGA ACTTCTTGCA CTTCTCAAAG ATGACTTAAC CCCAGTAGAG 
AAGGCTTATG TGAGAAAAAG TATTGAACAA CATAAGTTAG GCACCAACAA AGCGTTACTT 
GGCCAGGTCC GTGTAGTTGG TGCAACCTGT GCTGCCTGTC CTTTTGCTTG TATGAGCAAT 
CTAAAATTTC CTGTTGTAGT TCTGGATGAG TGTAGCCAGA TGACAGAACC AGCATCTATG 
CTTCCAGTTG CAAGATTTCA GTGTGAAAAA CTAATTCTTG TTGGGGATCC AAAGCAGTTG 
TCTCCAACTA TTCAGGGCTC TGAGCCAGCA CATGAAAAAG GTCTAGAACA GACACTCTTC 
AGTCGGCTTT GTCTGATGGG CCATAAAGCT ATAATTTTAA GAACACAGTA TCGATGTCAT 
CCTGCTATTT GCTCCATAGC TAATGAACTG TTCTATGACA GCCATCTCAT AAATGGTGTA 
TCGGAAGAAG ACAGAAAACC TCTTCTGGAC TGGCTGCCAA CATTATGCTT TTATAATGTG 
AATGGCACAG AACAGGTTGA GGGAAACAAC AGCTTTTATA ATATGGAAGA GGCCAACTTT 
ACAGTTAAGC TTATTCAGTC TCTTATTGCA AGTGGAATTG AGGGATCTAT GATTGGTGTG 
ATCACTCTAT ACAAGTCACA GATGTACAAG ATTTTTAGCT TACTTGCCAG CAGTGCACAC 
TGTGATTCTA CAGATATAAA AGCAGTTCAG GTTTCAACGG TTGATGCATT CCAAGGAGCC 
GAGAAGGAAA TAATTATCTT GTCCTGTGTT CGGACAAGGC AAGTTGGATT CATAGATTCA 
GAAAAAAGAA TGAATGTTGG GCTGACCAGG GGAAAGAGGC ATTTATTGAT CATTGCTAAT 
CTCGCCTGCT TGAGGAAGAA CAAACTTTGG GAACACGTGA TCCATCACTG TGAAAGGCAG 
AAAAATGGTT TAAAACATGT GAGTCAGTGG GAAGAAAAAC TTAATGCAAT CCTTTTGCGC 
TACCAAGAGA AGAAAATAGA AGAAGACATG AAAAAGAAAA AATCCAAAGC AAGAGAGTGT 
AATGTAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758668.1
CDS	217..6048
Translation	MAAQAFIVLYTHQKTKKSKTWQDGILKLSPEGRVATLYNDKGQHLENVYLKTNKLNSGDDLESDRYLITVEGEDLSGGIPQTSTEVREAPKISKSTLKPVGLLHPVGLKRKFSGFQGPREISKKPSLGVEGETQSPVLAQGCSSLPSQLYSASPLFATPCLKNSDAILPPDCGSLACWKNNPDGRNQTLPLTSSFSCSYSSNDLWMTESEKVDKSSSTAANVEVNSVTQKFRSRSQILALLKSQPPQQTSAALSKSNEHYDELSGSGHSDVLNTKDTIMPGKENCFVVVGQKSKYPQDIPVEPNLKKSRWDAYLEHSPPSDSEDTVNSDKDKSVEQDDPYKVQCFSSSLDLTQKTSSRKNIQGKPLSPQLSAQSENCLPPQLAEATKFHTPQTSVLHLQWKTETSNCKSYVGQEDLNTDNCSNATQPFGDISFSSEYSLQGCRDNETPISEKPVASPSLKISDTAICSSEDETSDPAMTEITFNLLDSFDCIVSDDEDSQIVCPNIKEAEHGPLGEIIPTKDRKDNVPLHCQNEKHAGNEIMESRDITDMSSGSNISRTKNYGLVEYDSKLQVLSDTVKHENCAIKEEHKEKLQNFPGTSDLGRMFDIKTELSHDKKDTTENTAHISMGNIVQPKGSLLENSTVVKEASKLSQESQRSTHNCILVLPINKSQSKMPLLENHIQSSNEGILCNQSTFDSTENIKTENSISVLKGLTKHSNAIESLDILNDDSESTTREDECSGHTDKLSGEVEEDHHKTMHDDFFQCDLDQAKPFPTAEFIFSQEFKSDNTPRFQCPEPVPPKLLSAKSLEEELFVEDFPAKPGTVHVPQGTHCNNAVPFKAFALPQWDGGTQQSDCDWDLSQWPSRETIKPDLTIVQEKPDIDRPFSWHENTIMQLKELKDTQNATQDISCTNSTISQVGSKLQRLLPVVTTPDLADSTRPVPAGYEYKKCFLDTNCSPSPNIYTTKNLIDVPVHNAFVENIEEGTGFLKPTEDRNSPKRQSKWTKYQNKSLCSRTNENVEDMKANEEFFAKSLFGKEPYMADKVDDKASVESICLTLLKDTLSKKNSGLVQHGLHTKRHPTISIAQLNKGKTSFLSDSSGNVKISCELHFPSRETVQSTVSIPKREISIPILYQSVTHYKHVFTAALTEHLNIMMFELSQRLYKALSKVDMSFYTSSRAAGEMGKHNVTPLCLHQQPAKLVMVKKEGPNKGRFFYTCDAPKSDQCKFFKWLNETQCSSPVQGHAQPKVVMGDMKSVASFVRSHKVALYEESHLMVRKVSGFQNKSFGKFKKIINANSDFAGESKTKLYLKLNRKENSSAYSKDDLWVVSKTIDFEPVDTFVAFSVFFGPSANNEIEIQPLKGYSPSNWPSNTLVHAVLVCNASTELTSLRNIQEHLNPSTLPIMPHLLKMQSEQEGESKTNTGKFKPPSLTAKIPQRCELPSCSFILAKSQEMIEQFRLNEDQATALMQIAQMMTTAAGSYKAVAPPITVIHGVFGAGKSYLLSVVVLFLVQLFERGNPAEGYEASPWKILISSSTNVAVDRVLLGLLDLGFSQFVRVGSIRKIAKPILPFSLHAGSENENEQIKELLALLKDDLTPVEKAYVRKSIEQHKLGTNKALLGQVRVVGATCAACPFACMSNLKFPVVVLDECSQMTEPASMLPVARFQCEKLILVGDPKQLSPTIQGSEPAHEKGLEQTLFSRLCLMGHKAIILRTQYRCHPAICSIANELFYDSHLINGVSEEDRKPLLDWLPTLCFYNVNGTEQVEGNNSFYNMEEANFTVKLIQSLIASGIEGSMIGVITLYKSQMYKIFSLLASSAHCDSTDIKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVGLTRGKRHLLIIANLACLRKNKLWEHVIHHCERQKNGLKHVSQWEEKLNAILLRYQEKKIEEDMKKKKSKARECNVR

Target ORF information:

RefSeq Version	XM_031902808.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902808.1

ORF Insert Sequence:

ATGGCTGCTC AGGCATTTAT CGTTTTGTAC ACTCATCAAA AGACTAAAAA GTCCAAAACA 
TGGCAAGATG GAATTTTAAA GCTATCACCA GAAGGGAGAG TGGCAACATT ATATAATGAC 
AAAGGACAAC ACCTGGAAAA TGTTTATTTG AAAACAAATA AGTTGAATTC AGGGGATGAT 
TTGGAAAGTG ACCGGTATTT AATCACAGTT GAAGGTGAAG ATTTAAGTGG TGGTATCCCA 
CAGACCTCTA CTGAAGTCAG AGAAGCTCCC AAAATCAGTA AAAGCACATT GAAACCTGTT 
GGTCTTCTTC ATCCTGTTGG CCTGAAAAGA AAATTTTCTG GTTTTCAAGG TCCACGTGAA 
ATTTCCAAGA AGCCGTCTCT GGGGGTAGAG GGTGAAACCC AATCTCCAGT GCTCGCCCAA 
GGTTGTTCCT CTTTGCCTTC ACAGTTGTAC TCTGCATCTC CACTGTTTGC TACTCCTTGC 
TTAAAGAATT CAGATGCCAT TCTGCCACCT GATTGTGGTT CTCTTGCCTG CTGGAAAAAC 
AATCCTGATG GAAGAAATCA GACTTTACCA TTGACTTCTT CTTTTTCATG CTCATATTCA 
TCTAATGATT TGTGGATGAC TGAAAGTGAA AAGGTTGACA AGTCCTCTTC TACAGCCGCC 
AATGTGGAAG TGAATAGTGT CACACAAAAG TTTAGAAGCA GATCACAAAT TTTGGCACTG 
TTAAAGTCTC AACCTCCCCA GCAAACCAGT GCAGCATTAT CTAAATCTAA TGAGCACTAT 
GATGAACTTT CTGGTTCTGG GCATAGCGAT GTGTTAAATA CGAAGGATAC AATTATGCCT 
GGAAAAGAAA ACTGTTTTGT AGTTGTTGGA CAGAAATCTA AATATCCACA AGACATACCT 
GTTGAACCTA ATCTTAAAAA GAGTCGGTGG GATGCATATT TGGAGCATTC ACCACCCTCT 
GATTCTGAAG ATACTGTTAA TAGTGACAAG GATAAATCTG TTGAACAAGA TGACCCATAT 
AAGGTGCAAT GCTTTTCAAG TTCCTTAGAC TTGACTCAGA AAACTAGTTC AAGAAAGAAC 
ATTCAGGGCA AACCACTGAG TCCACAATTA TCAGCACAGT CTGAAAATTG CTTACCACCT 
CAGTTAGCTG AAGCAACCAA ATTCCACACT CCACAGACCA GTGTATTACA TTTGCAGTGG 
AAAACAGAAA CAAGTAATTG TAAAAGTTAT GTAGGACAAG AAGACTTGAA TACAGATAAT 
TGCTCAAATG CTACTCAACC ATTTGGGGAT ATCTCCTTTT CCTCTGAATA CAGTCTTCAA 
GGATGTAGAG ATAATGAGAC ACCCATATCA GAGAAACCTG TTGCTTCCCC ATCTCTGAAG 
ATATCTGATA CAGCTATATG TTCTTCTGAA GATGAAACCA GTGACCCAGC AATGACAGAA 
ATCACATTCA ACTTGTTGGA CAGTTTTGAT TGCATAGTCT CAGATGATGA GGATTCACAA 
ATAGTTTGCC CTAATATAAA AGAAGCAGAA CATGGCCCAC TTGGTGAAAT AATACCCACT 
AAAGATCGAA AGGACAATGT GCCTTTGCAT TGCCAGAATG AAAAACATGC AGGGAATGAA 
ATTATGGAAT CCAGAGATAT AACTGACATG TCATCTGGTT CTAATATCTC AAGAACTAAA 
AATTATGGTT TAGTGGAATA TGACAGCAAG TTACAAGTGC TATCTGACAC TGTTAAACAT 
GAGAACTGTG CAATAAAAGA GGAACACAAG GAAAAGCTGC AAAACTTCCC TGGAACATCT 
GATTTGGGTC GTATGTTTGA CATTAAAACT GAACTTTCCC ATGACAAAAA GGACACCACA 
GAAAACACTG CGCATATATC AATGGGAAAT ATTGTACAGC CTAAAGGCAG CCTTTTGGAA 
AACTCTACAG TAGTAAAGGA GGCCTCAAAG CTTTCACAAG AGTCACAAAG AAGCACACAT 
AATTGTATAT TAGTGCTTCC CATAAACAAG TCACAATCTA AAATGCCACT TTTGGAAAAT 
CATATTCAAA GTTCAAATGA AGGGATTTTA TGTAACCAAA GTACCTTTGA TTCCACTGAA 
AATATTAAAA CAGAAAACAG CATCTCTGTT CTGAAAGGAC TGACAAAACA TAGTAATGCA 
ATAGAGAGCC TAGACATTTT AAATGATGAT TCAGAATCAA CAACTCGAGA AGATGAGTGT 
TCAGGACACA CAGATAAGCT GAGTGGAGAA GTAGAAGAAG ATCATCACAA GACTATGCAT 
GATGATTTTT TCCAGTGTGA CCTTGACCAA GCTAAACCTT TTCCCACTGC AGAATTCATA 
TTTTCACAAG AGTTTAAGTC TGACAATACT CCCCGTTTCC AGTGTCCTGA ACCTGTCCCA 
CCTAAGCTGC TATCTGCTAA ATCTTTAGAG GAAGAATTGT TTGTTGAGGA TTTTCCAGCC 
AAGCCTGGTA CAGTGCATGT ACCTCAAGGA ACCCATTGTA ATAATGCTGT CCCATTTAAA 
GCATTTGCTC TGCCTCAGTG GGATGGAGGC ACTCAGCAAT CAGATTGTGA TTGGGACCTT 
TCACAGTGGC CATCCAGAGA AACAATCAAG CCAGACCTTA CAATAGTACA AGAAAAGCCA 
GATATAGATC GGCCATTTTC ATGGCATGAA AATACAATTA TGCAGTTAAA AGAATTAAAG 
GACACACAAA ATGCCACCCA AGACATCAGT TGTACAAACA GCACAATTTC CCAAGTTGGG 
TCAAAGCTTC AGAGACTATT GCCAGTCGTA ACAACACCAG ACCTTGCAGA CTCTACCAGG 
CCAGTACCAG CAGGGTATGA ATACAAAAAA TGCTTTTTGG ACACAAATTG TAGCCCATCT 
CCCAATATAT ATACAACCAA GAACTTGATA GATGTCCCTG TACATAATGC TTTTGTTGAG 
AACATTGAAG AGGGCACAGG TTTCCTGAAA CCAACAGAGG ATAGAAATTC ACCAAAAAGA 
CAAAGCAAAT GGACAAAATA TCAAAACAAA TCTCTTTGCA GCAGAACAAA TGAAAATGTA 
GAAGATATGA AAGCAAATGA GGAGTTTTTT GCAAAGTCTT TGTTTGGAAA AGAACCATAT 
ATGGCAGACA AAGTGGATGA TAAGGCCTCT GTGGAATCTA TTTGTCTGAC TTTGTTAAAG 
GACACCCTGA GTAAAAAGAA TAGTGGTCTT GTGCAGCATG GGCTTCACAC CAAACGCCAC 
CCTACTATTA GTATTGCACA GCTTAATAAA GGCAAAACAA GTTTCCTTTC CGATTCATCA 
GGCAATGTAA AGATTTCCTG TGAGCTACAT TTTCCTAGTA GGGAAACTGT ACAGTCTACT 
GTGAGCATTC CCAAAAGAGA AATTTCCATA CCTATTCTGT ACCAATCGGT GACACATTAC 
AAACATGTCT TCACTGCAGC ACTCACAGAA CATTTGAACA TAATGATGTT TGAACTATCA 
CAACGGCTCT ACAAAGCTCT CTCCAAAGTT GATATGTCTT TCTACACATC ATCCAGAGCA 
GCAGGAGAGA TGGGTAAACA CAATGTTACT CCCCTGTGCC TGCACCAACA ACCAGCAAAA 
CTAGTTATGG TGAAAAAAGA GGGTCCAAAC AAGGGTCGTT TCTTTTACAC TTGTGATGCT 
CCCAAAAGTG ACCAGTGTAA ATTCTTTAAA TGGTTGAACG AAACTCAGTG TTCAAGCCCT 
GTACAAGGAC ATGCGCAACC CAAGGTTGTA ATGGGTGATA TGAAAAGTGT TGCCAGTTTT 
GTGAGAAGTC ACAAAGTAGC CCTTTACGAG GAGAGCCATC TAATGGTTAG GAAAGTATCT 
GGATTTCAAA ATAAAAGCTT TGGTAAGTTT AAGAAAATAA TTAATGCCAA CTCTGACTTT 
GCTGGTGAAT CTAAAACCAA ACTTTACCTG AAACTGAACA GAAAGGAAAA CTCTTCAGCT 
TACAGCAAAG ATGATTTGTG GGTTGTTTCA AAAACGATTG ACTTTGAACC AGTGGATACT 
TTCGTTGCTT TCAGTGTTTT CTTTGGTCCA TCTGCTAACA ATGAAATAGA GATTCAGCCT 
CTGAAAGGAT ACAGCCCATC CAACTGGCCA TCAAATACAC TTGTCCATGC AGTGTTGGTT 
TGTAATGCAA GCACAGAACT GACATCTCTG AGAAATATAC AGGAGCACCT AAACCCATCC 
ACACTGCCAA TCATGCCACA TCTATTGAAA ATGCAATCTG AACAGGAAGG AGAATCTAAA 
ACAAACACTG GAAAGTTCAA GCCACCATCA CTCACTGCAA AGATCCCCCA AAGATGTGAA 
CTTCCAAGCT GTAGTTTTAT ACTGGCAAAA TCACAGGAGA TGATTGAGCA GTTTAGATTG 
AATGAAGATC AAGCCACAGC CTTGATGCAG ATAGCACAGA TGATGACTAC TGCTGCAGGA 
TCTTATAAAG CAGTGGCTCC ACCAATAACA GTTATTCATG GTGTTTTTGG TGCTGGAAAA 
AGCTATCTGC TCTCTGTGGT TGTTCTGTTT CTAGTGCAGT TGTTTGAAAG GGGAAATCCA 
GCAGAAGGTT ATGAAGCATC TCCTTGGAAA ATTCTCATAT CTTCATCCAC CAATGTGGCA 
GTTGATAGAG TCCTCCTAGG ATTGCTTGAT CTCGGATTTA GCCAGTTTGT AAGAGTTGGA 
AGCATTAGAA AAATTGCAAA GCCCATCCTT CCTTTCAGTC TGCATGCTGG GTCTGAAAAT 
GAAAATGAGC AAATAAAAGA ACTTCTTGCA CTTCTCAAAG ATGACTTAAC CCCAGTAGAG 
AAGGCTTATG TGAGAAAAAG TATTGAACAA CATAAGTTAG GCACCAACAA AGCGTTACTT 
GGCCAGGTCC GTGTAGTTGG TGCAACCTGT GCTGCCTGTC CTTTTGCTTG TATGAGCAAT 
CTAAAATTTC CTGTTGTAGT TCTGGATGAG TGTAGCCAGA TGACAGAACC AGCATCTATG 
CTTCCAGTTG CAAGATTTCA GTGTGAAAAA CTAATTCTTG TTGGGGATCC AAAGCAGTTG 
TCTCCAACTA TTCAGGGCTC TGAGCCAGCA CATGAAAAAG GTCTAGAACA GACACTCTTC 
AGTCGGCTTT GTCTGATGGG CCATAAAGCT ATAATTTTAA GAACACAGTA TCGATGTCAT 
CCTGCTATTT GCTCCATAGC TAATGAACTG TTCTATGACA GCCATCTCAT AAATGGTGTA 
TCGGAAGAAG ACAGAAAACC TCTTCTGGAC TGGCTGCCAA CATTATGCTT TTATAATGTG 
AATGGCACAG AACAGGTTGA GGGAAACAAC AGCTTTTATA ATATGGAAGA GGCCAACTTT 
ACAGTTAAGC TTATTCAGTC TCTTATTGCA AGTGGAATTG AGGGATCTAT GATTGGTGTG 
ATCACTCTAT ACAAGTCACA GATGTACAAG ATTTTTAGCT TACTTGCCAG CAGTGCACAC 
TGTGATTCTA CAGATATAAA AGCAGTTCAG GTTTCAACGG TTGATGCATT CCAAGGAGCC 
GAGAAGGAAA TAATTATCTT GTCCTGTGTT CGGACAAGGC AAGTTGGATT CATAGATTCA 
GAAAAAAGAA TGAATGTTGG GCTGACCAGG GGAAAGAGGC ATTTATTGAT CATTGCTAAT 
CTCGCCTGCT TGAGGAAGAA CAAACTTTGG GAACACGTGA TCCATCACTG TGAAAGGCAG 
AAAAATGGTT TAAAACATGT GAGTCAGTGG GAAGAAAAAC TTAATGCAAT CCTTTTGCGC 
TACCAAGAGA AGAAAATAGA AGAAGACATG AAAAAGAAAA AATCCAAAGC AAGAGAGTGT 
AATGTAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgrf1 ( XM_031902812.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758672.1 Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X5, mRNA.

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CloneID	OXa48131
Clone ID Related Accession (Same CDS sequence)	XM_031902812.1
Accession Version	XM_031902812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein ZGRF1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC AGGCATTTAT CGTTTTGTAC ACTCATCAAA AGACTAAAAA GTCCAAAACA 
TGGCAAGATG GAATTTTAAA GCTATCACCA GAAGGGAGAG TGGCAACATT ATATAATGAC 
AAAGGACAAC ACCTGGAAAA TGTTTATTTG AAAACAAATA AGGGTTTTCA AGGTCCACGT 
GAAATTTCCA AGAAGCCGTC TCTGGGGGTA GAGGGTGAAA CCCAATCTCC AGTGCTCGCC 
CAAGGTTGTT CCTCTTTGCC TTCACAGTTG TACTCTGCAT CTCCACTGTT TGCTACTCCT 
TGCTTAAAGA ATTCAGATGC CATTCTGCCA CCTGATTGTG GTTCTCTTGC CTGCTGGAAA 
AACAATCCTG ATGGAAGAAA TCAGACTTTA CCATTGACTT CTTCTTTTTC ATGCTCATAT 
TCATCTAATG ATTTGTGGAT GACTGAAAGT GAAAAGGTTG ACAAGTCCTC TTCTACAGCC 
GCCAATGTGG AAGTGAATAG TGTCACACAA AAGTTTAGAA GCAGATCACA AATTTTGGCA 
CTGTTAAAGT CTCAACCTCC CCAGCAAACC AGTGCAGCAT TATCTAAATC TAATGAGCAC 
TATGATGAAC TTTCTGGTTC TGGGCATAGC GATGTGTTAA ATACGAAGGA TACAATTATG 
CCTGGAAAAG AAAACTGTTT TGTAGTTGTT GGACAGAAAT CTAAATATCC ACAAGACATA 
CCTGTTGAAC CTAATCTTAA AAAGAGTCGG TGGGATGCAT ATTTGGAGCA TTCACCACCC 
TCTGATTCTG AAGATACTGT TAATAGTGAC AAGGATAAAT CTGTTGAACA AGATGACCCA 
TATAAGGTGC AATGCTTTTC AAGTTCCTTA GACTTGACTC AGAAAACTAG TTCAAGAAAG 
AACATTCAGG GCAAACCACT GAGTCCACAA TTATCAGCAC AGTCTGAAAA TTGCTTACCA 
CCTCAGTTAG CTGAAGCAAC CAAATTCCAC ACTCCACAGA CCAGTGTATT ACATTTGCAG 
TGGAAAACAG AAACAAGTAA TTGTAAAAGT TATGTAGGAC AAGAAGACTT GAATACAGAT 
AATTGCTCAA ATGCTACTCA ACCATTTGGG GATATCTCCT TTTCCTCTGA ATACAGTCTT 
CAAGGATGTA GAGATAATGA GACACCCATA TCAGAGAAAC CTGTTGCTTC CCCATCTCTG 
AAGATATCTG ATACAGCTAT ATGTTCTTCT GAAGATGAAA CCAGTGACCC AGCAATGACA 
GAAATCACAT TCAACTTGTT GGACAGTTTT GATTGCATAG TCTCAGATGA TGAGGATTCA 
CAAATAGTTT GCCCTAATAT AAAAGAAGCA GAACATGGCC CACTTGGTGA AATAATACCC 
ACTAAAGATC GAAAGGACAA TGTGCCTTTG CATTGCCAGA ATGAAAAACA TGCAGGGAAT 
GAAATTATGG AATCCAGAGA TATAACTGAC ATGTCATCTG GTTCTAATAT CTCAAGAACT 
AAAAATTATG GTTTAGTGGA ATATGACAGC AAGTTACAAG TGCTATCTGA CACTGTTAAA 
CATGAGAACT GTGCAATAAA AGAGGAACAC AAGGAAAAGC TGCAAAACTT CCCTGGAACA 
TCTGATTTGG GTCGTATGTT TGACATTAAA ACTGAACTTT CCCATGACAA AAAGGACACC 
ACAGAAAACA CTGCGCATAT ATCAATGGGA AATATTGTAC AGCCTAAAGG CAGCCTTTTG 
GAAAACTCTA CAGTAGTAAA GGAGGCCTCA AAGCTTTCAC AAGAGTCACA AAGAAGCACA 
CATAATTGTA TATTAGTGCT TCCCATAAAC AAGTCACAAT CTAAAATGCC ACTTTTGGAA 
AATCATATTC AAAGTTCAAA TGAAGGGATT TTATGTAACC AAAGTACCTT TGATTCCACT 
GAAAATATTA AAACAGAAAA CAGCATCTCT GTTCTGAAAG GACTGACAAA ACATAGTAAT 
GCAATAGAGA GCCTAGACAT TTTAAATGAT GATTCAGAAT CAACAACTCG AGAAGATGAG 
TGTTCAGGAC ACACAGATAA GCTGAGTGGA GAAGTAGAAG AAGATCATCA CAAGACTATG 
CATGATGATT TTTTCCAGTG TGACCTTGAC CAAGCTAAAC CTTTTCCCAC TGCAGAATTC 
ATATTTTCAC AAGAGTTTAA GTCTGACAAT ACTCCCCGTT TCCAGTGTCC TGAACCTGTC 
CCACCTAAGC TGCTATCTGC TAAATCTTTA GAGGAAGAAT TGTTTGTTGA GGATTTTCCA 
GCCAAGCCTG GTACAGTGCA TGTACCTCAA GGAACCCATT GTAATAATGC TGTCCCATTT 
AAAGCATTTG CTCTGCCTCA GTGGGATGGA GGCACTCAGC AATCAGATTG TGATTGGGAC 
CTTTCACAGT GGCCATCCAG AGAAACAATC AAGCCAGACC TTACAATAGT ACAAGAAAAG 
CCAGATATAG ATCGGCCATT TTCATGGCAT GAAAATACAA TTATGCAGTT AAAAGAATTA 
AAGGACACAC AAAATGCCAC CCAAGACATC AGTTGTACAA ACAGCACAAT TTCCCAAGTT 
GGGTCAAAGC TTCAGAGACT ATTGCCAGTC GTAACAACAC CAGACCTTGC AGACTCTACC 
AGGCCAGTAC CAGCAGGGTA TGAATACAAA AAATGCTTTT TGGACACAAA TTGTAGCCCA 
TCTCCCAATA TATATACAAC CAAGAACTTG ATAGATGTCC CTGTACATAA TGCTTTTGTT 
GAGAACATTG AAGAGGGCAC AGGTTTCCTG AAACCAACAG AGGATAGAAA TTCACCAAAA 
AGACAAAGCA AATGGACAAA ATATCAAAAC AAATCTCTTT GCAGCAGAAC AAATGAAAAT 
GTAGAAGATA TGAAAGCAAA TGAGGAGTTT TTTGCAAAGT CTTTGTTTGG AAAAGAACCA 
TATATGGCAG ACAAAGTGGA TGATAAGGCC TCTGTGGAAT CTATTTGTCT GACTTTGTTA 
AAGGACACCC TGAGTAAAAA GAATAGTGGT CTTGTGCAGC ATGGGCTTCA CACCAAACGC 
CACCCTACTA TTAGTATTGC ACAGCTTAAT AAAGGCAAAA CAAGTTTCCT TTCCGATTCA 
TCAGGCAATG TAAAGATTTC CTGTGAGCTA CATTTTCCTA GTAGGGAAAC TGTACAGTCT 
ACTGTGAGCA TTCCCAAAAG AGAAATTTCC ATACCTATTC TGTACCAATC GGTGACACAT 
TACAAACATG TCTTCACTGC AGCACTCACA GAACATTTGA ACATAATGAT GTTTGAACTA 
TCACAACGGC TCTACAAAGC TCTCTCCAAA GTTGATATGT CTTTCTACAC ATCATCCAGA 
GCAGCAGGAG AGATGGGTAA ACACAATGTT ACTCCCCTGT GCCTGCACCA ACAACCAGCA 
AAACTAGTTA TGGTGAAAAA AGAGGGTCCA AACAAGGGTC GTTTCTTTTA CACTTGTGAT 
GCTCCCAAAA GTGACCAGTG TAAATTCTTT AAATGGTTGA ACGAAACTCA GTGTTCAAGC 
CCTGTACAAG GACATGCGCA ACCCAAGGTT GTAATGGGTG ATATGAAAAG TGTTGCCAGT 
TTTGTGAGAA GTCACAAAGT AGCCCTTTAC GAGGAGAGCC ATCTAATGGT TAGGAAAGTA 
TCTGGATTTC AAAATAAAAG CTTTGGTAAG TTTAAGAAAA TAATTAATGC CAACTCTGAC 
TTTGCTGGTG AATCTAAAAC CAAACTTTAC CTGAAACTGA ACAGAAAGGA AAACTCTTCA 
GCTTACAGCA AAGATGATTT GTGGGTTGTT TCAAAAACGA TTGACTTTGA ACCAGTGGAT 
ACTTTCGTTG CTTTCAGTGT TTTCTTTGGT CCATCTGCTA ACAATGAAAT AGAGATTCAG 
CCTCTGAAAG GATACAGCCC ATCCAACTGG CCATCAAATA CACTTGTCCA TGCAGTGTTG 
GTTTGTAATG CAAGCACAGA ACTGACATCT CTGAGAAATA TACAGGAGCA CCTAAACCCA 
TCCACACTGC CAATCATGCC ACATCTATTG AAAATGCAAT CTGAACAGGA AGGAGAATCT 
AAAACAAACA CTGGAAAGTT CAAGCCACCA TCACTCACTG CAAAGATCCC CCAAAGATGT 
GAACTTCCAA GCTGTAGTTT TATACTGGCA AAATCACAGG AGATGATTGA GCAGTTTAGA 
TTGAATGAAG ATCAAGCCAC AGCCTTGATG CAGATAGCAC AGATGATGAC TACTGCTGCA 
GGATCTTATA AAGCAGTGGC TCCACCAATA ACAGTTATTC ATGGTGTTTT TGGTGCTGGA 
AAAAGCTATC TGCTCTCTGT GGTTGTTCTG TTTCTAGTGC AGTTGTTTGA AAGGGGAAAT 
CCAGCAGAAG GTTATGAAGC ATCTCCTTGG AAAATTCTCA TATCTTCATC CACCAATGTG 
GCAGTTGATA GAGTCCTCCT AGGATTGCTT GATCTCGGAT TTAGCCAGTT TGTAAGAGTT 
GGAAGCATTA GAAAAATTGC AAAGCCCATC CTTCCTTTCA GTCTGCATGC TGGGTCTGAA 
AATGAAAATG AGCAAATAAA AGAACTTCTT GCACTTCTCA AAGATGACTT AACCCCAGTA 
GAGAAGGCTT ATGTGAGAAA AAGTATTGAA CAACATAAGT TAGGCACCAA CAAAGCGTTA 
CTTGGCCAGG TCCGTGTAGT TGGTGCAACC TGTGCTGCCT GTCCTTTTGC TTGTATGAGC 
AATCTAAAAT TTCCTGTTGT AGTTCTGGAT GAGTGTAGCC AGATGACAGA ACCAGCATCT 
ATGCTTCCAG TTGCAAGATT TCAGTGTGAA AAACTAATTC TTGTTGGGGA TCCAAAGCAG 
TTGTCTCCAA CTATTCAGGG CTCTGAGCCA GCACATGAAA AAGGTCTAGA ACAGACACTC 
TTCAGTCGGC TTTGTCTGAT GGGCCATAAA GCTATAATTT TAAGAACACA GTATCGATGT 
CATCCTGCTA TTTGCTCCAT AGCTAATGAA CTGTTCTATG ACAGCCATCT CATAAATGGT 
GTATCGGAAG AAGACAGAAA ACCTCTTCTG GACTGGCTGC CAACATTATG CTTTTATAAT 
GTGAATGGCA CAGAACAGGT TGAGGGAAAC AACAGCTTTT ATAATATGGA AGAGGCCAAC 
TTTACAGTTA AGCTTATTCA GTCTCTTATT GCAAGTGGAA TTGAGGGATC TATGATTGGT 
GTGATCACTC TATACAAGTC ACAGATGTAC AAGATTTTTA GCTTACTTGC CAGCAGTGCA 
CACTGTGATT CTACAGATAT AAAAGCAGTT CAGGTTTCAA CGGTTGATGC ATTCCAAGGA 
GCCGAGAAGG AAATAATTAT CTTGTCCTGT GTTCGGACAA GGCAAGTTGG ATTCATAGAT 
TCAGAAAAAA GAATGAATGT TGGGCTGACC AGGGGAAAGA GGCATTTATT GATCATTGCT 
AATCTCGCCT GCTTGAGGAA GAACAAACTT TGGGAACACG TGATCCATCA CTGTGAAAGG 
CAGAAAAATG GTTTAAAACA TGTGAGTCAG TGGGAAGAAA AACTTAATGC AATCCTTTTG 
CGCTACCAAG AGAAGAAAAT AGAAGAAGAC ATGAAAAAGA AAAAATCCAA AGCAAGAGAG 
TGTAATGTAA ACCCTTCTCC ACAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758672.1
CDS	1639..7308
Translation	MAAQAFIVLYTHQKTKKSKTWQDGILKLSPEGRVATLYNDKGQHLENVYLKTNKGFQGPREISKKPSLGVEGETQSPVLAQGCSSLPSQLYSASPLFATPCLKNSDAILPPDCGSLACWKNNPDGRNQTLPLTSSFSCSYSSNDLWMTESEKVDKSSSTAANVEVNSVTQKFRSRSQILALLKSQPPQQTSAALSKSNEHYDELSGSGHSDVLNTKDTIMPGKENCFVVVGQKSKYPQDIPVEPNLKKSRWDAYLEHSPPSDSEDTVNSDKDKSVEQDDPYKVQCFSSSLDLTQKTSSRKNIQGKPLSPQLSAQSENCLPPQLAEATKFHTPQTSVLHLQWKTETSNCKSYVGQEDLNTDNCSNATQPFGDISFSSEYSLQGCRDNETPISEKPVASPSLKISDTAICSSEDETSDPAMTEITFNLLDSFDCIVSDDEDSQIVCPNIKEAEHGPLGEIIPTKDRKDNVPLHCQNEKHAGNEIMESRDITDMSSGSNISRTKNYGLVEYDSKLQVLSDTVKHENCAIKEEHKEKLQNFPGTSDLGRMFDIKTELSHDKKDTTENTAHISMGNIVQPKGSLLENSTVVKEASKLSQESQRSTHNCILVLPINKSQSKMPLLENHIQSSNEGILCNQSTFDSTENIKTENSISVLKGLTKHSNAIESLDILNDDSESTTREDECSGHTDKLSGEVEEDHHKTMHDDFFQCDLDQAKPFPTAEFIFSQEFKSDNTPRFQCPEPVPPKLLSAKSLEEELFVEDFPAKPGTVHVPQGTHCNNAVPFKAFALPQWDGGTQQSDCDWDLSQWPSRETIKPDLTIVQEKPDIDRPFSWHENTIMQLKELKDTQNATQDISCTNSTISQVGSKLQRLLPVVTTPDLADSTRPVPAGYEYKKCFLDTNCSPSPNIYTTKNLIDVPVHNAFVENIEEGTGFLKPTEDRNSPKRQSKWTKYQNKSLCSRTNENVEDMKANEEFFAKSLFGKEPYMADKVDDKASVESICLTLLKDTLSKKNSGLVQHGLHTKRHPTISIAQLNKGKTSFLSDSSGNVKISCELHFPSRETVQSTVSIPKREISIPILYQSVTHYKHVFTAALTEHLNIMMFELSQRLYKALSKVDMSFYTSSRAAGEMGKHNVTPLCLHQQPAKLVMVKKEGPNKGRFFYTCDAPKSDQCKFFKWLNETQCSSPVQGHAQPKVVMGDMKSVASFVRSHKVALYEESHLMVRKVSGFQNKSFGKFKKIINANSDFAGESKTKLYLKLNRKENSSAYSKDDLWVVSKTIDFEPVDTFVAFSVFFGPSANNEIEIQPLKGYSPSNWPSNTLVHAVLVCNASTELTSLRNIQEHLNPSTLPIMPHLLKMQSEQEGESKTNTGKFKPPSLTAKIPQRCELPSCSFILAKSQEMIEQFRLNEDQATALMQIAQMMTTAAGSYKAVAPPITVIHGVFGAGKSYLLSVVVLFLVQLFERGNPAEGYEASPWKILISSSTNVAVDRVLLGLLDLGFSQFVRVGSIRKIAKPILPFSLHAGSENENEQIKELLALLKDDLTPVEKAYVRKSIEQHKLGTNKALLGQVRVVGATCAACPFACMSNLKFPVVVLDECSQMTEPASMLPVARFQCEKLILVGDPKQLSPTIQGSEPAHEKGLEQTLFSRLCLMGHKAIILRTQYRCHPAICSIANELFYDSHLINGVSEEDRKPLLDWLPTLCFYNVNGTEQVEGNNSFYNMEEANFTVKLIQSLIASGIEGSMIGVITLYKSQMYKIFSLLASSAHCDSTDIKAVQVSTVDAFQGAEKEIIILSCVRTRQVGFIDSEKRMNVGLTRGKRHLLIIANLACLRKNKLWEHVIHHCERQKNGLKHVSQWEEKLNAILLRYQEKKIEEDMKKKKSKARECNVNPSPQS

Target ORF information:

RefSeq Version	XM_031902812.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis zinc finger, GRF-type containing 1 (zgrf1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902812.1

ORF Insert Sequence:

ATGGCTGCTC AGGCATTTAT CGTTTTGTAC ACTCATCAAA AGACTAAAAA GTCCAAAACA 
TGGCAAGATG GAATTTTAAA GCTATCACCA GAAGGGAGAG TGGCAACATT ATATAATGAC 
AAAGGACAAC ACCTGGAAAA TGTTTATTTG AAAACAAATA AGGGTTTTCA AGGTCCACGT 
GAAATTTCCA AGAAGCCGTC TCTGGGGGTA GAGGGTGAAA CCCAATCTCC AGTGCTCGCC 
CAAGGTTGTT CCTCTTTGCC TTCACAGTTG TACTCTGCAT CTCCACTGTT TGCTACTCCT 
TGCTTAAAGA ATTCAGATGC CATTCTGCCA CCTGATTGTG GTTCTCTTGC CTGCTGGAAA 
AACAATCCTG ATGGAAGAAA TCAGACTTTA CCATTGACTT CTTCTTTTTC ATGCTCATAT 
TCATCTAATG ATTTGTGGAT GACTGAAAGT GAAAAGGTTG ACAAGTCCTC TTCTACAGCC 
GCCAATGTGG AAGTGAATAG TGTCACACAA AAGTTTAGAA GCAGATCACA AATTTTGGCA 
CTGTTAAAGT CTCAACCTCC CCAGCAAACC AGTGCAGCAT TATCTAAATC TAATGAGCAC 
TATGATGAAC TTTCTGGTTC TGGGCATAGC GATGTGTTAA ATACGAAGGA TACAATTATG 
CCTGGAAAAG AAAACTGTTT TGTAGTTGTT GGACAGAAAT CTAAATATCC ACAAGACATA 
CCTGTTGAAC CTAATCTTAA AAAGAGTCGG TGGGATGCAT ATTTGGAGCA TTCACCACCC 
TCTGATTCTG AAGATACTGT TAATAGTGAC AAGGATAAAT CTGTTGAACA AGATGACCCA 
TATAAGGTGC AATGCTTTTC AAGTTCCTTA GACTTGACTC AGAAAACTAG TTCAAGAAAG 
AACATTCAGG GCAAACCACT GAGTCCACAA TTATCAGCAC AGTCTGAAAA TTGCTTACCA 
CCTCAGTTAG CTGAAGCAAC CAAATTCCAC ACTCCACAGA CCAGTGTATT ACATTTGCAG 
TGGAAAACAG AAACAAGTAA TTGTAAAAGT TATGTAGGAC AAGAAGACTT GAATACAGAT 
AATTGCTCAA ATGCTACTCA ACCATTTGGG GATATCTCCT TTTCCTCTGA ATACAGTCTT 
CAAGGATGTA GAGATAATGA GACACCCATA TCAGAGAAAC CTGTTGCTTC CCCATCTCTG 
AAGATATCTG ATACAGCTAT ATGTTCTTCT GAAGATGAAA CCAGTGACCC AGCAATGACA 
GAAATCACAT TCAACTTGTT GGACAGTTTT GATTGCATAG TCTCAGATGA TGAGGATTCA 
CAAATAGTTT GCCCTAATAT AAAAGAAGCA GAACATGGCC CACTTGGTGA AATAATACCC 
ACTAAAGATC GAAAGGACAA TGTGCCTTTG CATTGCCAGA ATGAAAAACA TGCAGGGAAT 
GAAATTATGG AATCCAGAGA TATAACTGAC ATGTCATCTG GTTCTAATAT CTCAAGAACT 
AAAAATTATG GTTTAGTGGA ATATGACAGC AAGTTACAAG TGCTATCTGA CACTGTTAAA 
CATGAGAACT GTGCAATAAA AGAGGAACAC AAGGAAAAGC TGCAAAACTT CCCTGGAACA 
TCTGATTTGG GTCGTATGTT TGACATTAAA ACTGAACTTT CCCATGACAA AAAGGACACC 
ACAGAAAACA CTGCGCATAT ATCAATGGGA AATATTGTAC AGCCTAAAGG CAGCCTTTTG 
GAAAACTCTA CAGTAGTAAA GGAGGCCTCA AAGCTTTCAC AAGAGTCACA AAGAAGCACA 
CATAATTGTA TATTAGTGCT TCCCATAAAC AAGTCACAAT CTAAAATGCC ACTTTTGGAA 
AATCATATTC AAAGTTCAAA TGAAGGGATT TTATGTAACC AAAGTACCTT TGATTCCACT 
GAAAATATTA AAACAGAAAA CAGCATCTCT GTTCTGAAAG GACTGACAAA ACATAGTAAT 
GCAATAGAGA GCCTAGACAT TTTAAATGAT GATTCAGAAT CAACAACTCG AGAAGATGAG 
TGTTCAGGAC ACACAGATAA GCTGAGTGGA GAAGTAGAAG AAGATCATCA CAAGACTATG 
CATGATGATT TTTTCCAGTG TGACCTTGAC CAAGCTAAAC CTTTTCCCAC TGCAGAATTC 
ATATTTTCAC AAGAGTTTAA GTCTGACAAT ACTCCCCGTT TCCAGTGTCC TGAACCTGTC 
CCACCTAAGC TGCTATCTGC TAAATCTTTA GAGGAAGAAT TGTTTGTTGA GGATTTTCCA 
GCCAAGCCTG GTACAGTGCA TGTACCTCAA GGAACCCATT GTAATAATGC TGTCCCATTT 
AAAGCATTTG CTCTGCCTCA GTGGGATGGA GGCACTCAGC AATCAGATTG TGATTGGGAC 
CTTTCACAGT GGCCATCCAG AGAAACAATC AAGCCAGACC TTACAATAGT ACAAGAAAAG 
CCAGATATAG ATCGGCCATT TTCATGGCAT GAAAATACAA TTATGCAGTT AAAAGAATTA 
AAGGACACAC AAAATGCCAC CCAAGACATC AGTTGTACAA ACAGCACAAT TTCCCAAGTT 
GGGTCAAAGC TTCAGAGACT ATTGCCAGTC GTAACAACAC CAGACCTTGC AGACTCTACC 
AGGCCAGTAC CAGCAGGGTA TGAATACAAA AAATGCTTTT TGGACACAAA TTGTAGCCCA 
TCTCCCAATA TATATACAAC CAAGAACTTG ATAGATGTCC CTGTACATAA TGCTTTTGTT 
GAGAACATTG AAGAGGGCAC AGGTTTCCTG AAACCAACAG AGGATAGAAA TTCACCAAAA 
AGACAAAGCA AATGGACAAA ATATCAAAAC AAATCTCTTT GCAGCAGAAC AAATGAAAAT 
GTAGAAGATA TGAAAGCAAA TGAGGAGTTT TTTGCAAAGT CTTTGTTTGG AAAAGAACCA 
TATATGGCAG ACAAAGTGGA TGATAAGGCC TCTGTGGAAT CTATTTGTCT GACTTTGTTA 
AAGGACACCC TGAGTAAAAA GAATAGTGGT CTTGTGCAGC ATGGGCTTCA CACCAAACGC 
CACCCTACTA TTAGTATTGC ACAGCTTAAT AAAGGCAAAA CAAGTTTCCT TTCCGATTCA 
TCAGGCAATG TAAAGATTTC CTGTGAGCTA CATTTTCCTA GTAGGGAAAC TGTACAGTCT 
ACTGTGAGCA TTCCCAAAAG AGAAATTTCC ATACCTATTC TGTACCAATC GGTGACACAT 
TACAAACATG TCTTCACTGC AGCACTCACA GAACATTTGA ACATAATGAT GTTTGAACTA 
TCACAACGGC TCTACAAAGC TCTCTCCAAA GTTGATATGT CTTTCTACAC ATCATCCAGA 
GCAGCAGGAG AGATGGGTAA ACACAATGTT ACTCCCCTGT GCCTGCACCA ACAACCAGCA 
AAACTAGTTA TGGTGAAAAA AGAGGGTCCA AACAAGGGTC GTTTCTTTTA CACTTGTGAT 
GCTCCCAAAA GTGACCAGTG TAAATTCTTT AAATGGTTGA ACGAAACTCA GTGTTCAAGC 
CCTGTACAAG GACATGCGCA ACCCAAGGTT GTAATGGGTG ATATGAAAAG TGTTGCCAGT 
TTTGTGAGAA GTCACAAAGT AGCCCTTTAC GAGGAGAGCC ATCTAATGGT TAGGAAAGTA 
TCTGGATTTC AAAATAAAAG CTTTGGTAAG TTTAAGAAAA TAATTAATGC CAACTCTGAC 
TTTGCTGGTG AATCTAAAAC CAAACTTTAC CTGAAACTGA ACAGAAAGGA AAACTCTTCA 
GCTTACAGCA AAGATGATTT GTGGGTTGTT TCAAAAACGA TTGACTTTGA ACCAGTGGAT 
ACTTTCGTTG CTTTCAGTGT TTTCTTTGGT CCATCTGCTA ACAATGAAAT AGAGATTCAG 
CCTCTGAAAG GATACAGCCC ATCCAACTGG CCATCAAATA CACTTGTCCA TGCAGTGTTG 
GTTTGTAATG CAAGCACAGA ACTGACATCT CTGAGAAATA TACAGGAGCA CCTAAACCCA 
TCCACACTGC CAATCATGCC ACATCTATTG AAAATGCAAT CTGAACAGGA AGGAGAATCT 
AAAACAAACA CTGGAAAGTT CAAGCCACCA TCACTCACTG CAAAGATCCC CCAAAGATGT 
GAACTTCCAA GCTGTAGTTT TATACTGGCA AAATCACAGG AGATGATTGA GCAGTTTAGA 
TTGAATGAAG ATCAAGCCAC AGCCTTGATG CAGATAGCAC AGATGATGAC TACTGCTGCA 
GGATCTTATA AAGCAGTGGC TCCACCAATA ACAGTTATTC ATGGTGTTTT TGGTGCTGGA 
AAAAGCTATC TGCTCTCTGT GGTTGTTCTG TTTCTAGTGC AGTTGTTTGA AAGGGGAAAT 
CCAGCAGAAG GTTATGAAGC ATCTCCTTGG AAAATTCTCA TATCTTCATC CACCAATGTG 
GCAGTTGATA GAGTCCTCCT AGGATTGCTT GATCTCGGAT TTAGCCAGTT TGTAAGAGTT 
GGAAGCATTA GAAAAATTGC AAAGCCCATC CTTCCTTTCA GTCTGCATGC TGGGTCTGAA 
AATGAAAATG AGCAAATAAA AGAACTTCTT GCACTTCTCA AAGATGACTT AACCCCAGTA 
GAGAAGGCTT ATGTGAGAAA AAGTATTGAA CAACATAAGT TAGGCACCAA CAAAGCGTTA 
CTTGGCCAGG TCCGTGTAGT TGGTGCAACC TGTGCTGCCT GTCCTTTTGC TTGTATGAGC 
AATCTAAAAT TTCCTGTTGT AGTTCTGGAT GAGTGTAGCC AGATGACAGA ACCAGCATCT 
ATGCTTCCAG TTGCAAGATT TCAGTGTGAA AAACTAATTC TTGTTGGGGA TCCAAAGCAG 
TTGTCTCCAA CTATTCAGGG CTCTGAGCCA GCACATGAAA AAGGTCTAGA ACAGACACTC 
TTCAGTCGGC TTTGTCTGAT GGGCCATAAA GCTATAATTT TAAGAACACA GTATCGATGT 
CATCCTGCTA TTTGCTCCAT AGCTAATGAA CTGTTCTATG ACAGCCATCT CATAAATGGT 
GTATCGGAAG AAGACAGAAA ACCTCTTCTG GACTGGCTGC CAACATTATG CTTTTATAAT 
GTGAATGGCA CAGAACAGGT TGAGGGAAAC AACAGCTTTT ATAATATGGA AGAGGCCAAC 
TTTACAGTTA AGCTTATTCA GTCTCTTATT GCAAGTGGAA TTGAGGGATC TATGATTGGT 
GTGATCACTC TATACAAGTC ACAGATGTAC AAGATTTTTA GCTTACTTGC CAGCAGTGCA 
CACTGTGATT CTACAGATAT AAAAGCAGTT CAGGTTTCAA CGGTTGATGC ATTCCAAGGA 
GCCGAGAAGG AAATAATTAT CTTGTCCTGT GTTCGGACAA GGCAAGTTGG ATTCATAGAT 
TCAGAAAAAA GAATGAATGT TGGGCTGACC AGGGGAAAGA GGCATTTATT GATCATTGCT 
AATCTCGCCT GCTTGAGGAA GAACAAACTT TGGGAACACG TGATCCATCA CTGTGAAAGG 
CAGAAAAATG GTTTAAAACA TGTGAGTCAG TGGGAAGAAA AACTTAATGC AATCCTTTTG 
CGCTACCAAG AGAAGAAAAT AGAAGAAGAC ATGAAAAAGA AAAAATCCAA AGCAAGAGAG 
TGTAATGTAA ACCCTTCTCC ACAGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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zgrf1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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