brd4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	brd4
Entrez Gene ID	100216259
Full Name	bromodomain containing 4
General protein information	Preferred Names bromodomain containing 4 Names bromodomain-containing protein 4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012953714.2	XP_012809168.1	bromodomain-containing protein 4 isoform X2
NM_001142218.1	NP_001135690.1	bromodomain-containing protein 4
XM_012953713.2	XP_012809167.1	bromodomain-containing protein 4 isoform X2
XM_018091980.2	XP_017947469.1	bromodomain-containing protein 4 isoform X1
XM_018091980.1	XP_017947469.1	bromodomain-containing protein 4 isoform X1
XM_012953715.2	XP_012809169.2	bromodomain-containing protein 4 isoform X3
XM_012953714.1	XP_012809168.1	bromodomain-containing protein 4 isoform X2
XM_012953713.3	XP_012809167.1	bromodomain-containing protein 4 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Brd4	NP_065254.3
Xenopus tropicalis (tropical clawed frog)	brd4	NP_001135690.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following brd4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brd4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa32766	XM_012953713.3 Latest version!	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa32766	XM_012953714.2 Latest version!	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa43062	XM_018091980.2 Latest version!	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa43063	XM_012953715.2 Latest version!	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OXa23651	NM_001142218.1 Latest version!	Xenopus tropicalis bromodomain containing 4 (brd4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OXa43062	XM_018091980.1	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa32766	XM_012953714.1	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
OXa32766	XM_012953713.2	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$797.30 $1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brd4 ( XM_012953713.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809167.1 Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa32766
Clone ID Related Accession (Same CDS sequence)	XM_012953714.2 , XM_012953713.2 , XM_012953714.1 , XM_012953713.3
Accession Version	XM_012953713.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACCG AGACTGGGCC TGGTACACGT CTGAGAGCCA CATCTGGAAT GGGTGACGGA 
GTAGAAGGGG CTCAGATGTC GGGCCAACAG CTGCCTGCCG CTCCCCAGCC CCAGCCACAG 
AACTCATCGC TGCTCAATCC TCCTCCTCCT GATATCGCAA GACCCAGCCA AGCAAAGCGC 
CAGACCAACC AGCTGCAGTA CTTACTTAAG ACAGTGCTCA AGACATTGTG GAAGCACCAG 
TTTGCATGGC CTTTCCAGCT ACCTGTTGAT GTGGTCAAGC TCAATCTGCC TGATTATCAT 
AAAATAATTA AAAACCCAAT GGACATGGGC ACCATTAAAA AGCGCCTCGA GAACCATTAT 
TACTGGAACG CCCAAGAATG TATCCAGGAC TTCAACACAA TGTTTACAAA CTGCTACATC 
TATAACAAGC CTGGAGATGA CATAGTGCTG ATGGCCGAAG CCCTTGAGAA GCTTTTTCTG 
CAGAAGATCT CAGAGATGCC CCAGGAAGAA ACCGAACTGG CAGTTGTGCA GAGCAAAGGC 
CGTGGCCGGG GAAGAAAGGA GCAAAGTGAG CAAGATACTT CAATCACCCC CATGAGAACC 
CGAGTCCTGT CTGGGTCACT AGGAGACAAG AGCGCCGTCA AACCTCCCCC TGTTACACCT 
GTGTCCAAGC CTGCAACCCC AACGCCCCCT ACTGTGACCA GGGCTCCCAC CCCTCCACAA 
ACCAGACCAC CCCAACAAGC TCGCCCTCCA GCTATCACTC AAGCCCCCAT CAGTTTCTCT 
CCCACCATCA GTCAGGATGT GGTGGTTCCA ACGACCGTAG CCCCAACACC AGTTCCTCAG 
CCACTTCCAA ATCATCCTGC AGTCATCCAC ACTGCTGCCC AGCCTGCAAA GACAAAGAAA 
GGTGTGAAGA GGAAAGCAGA CACCACAACC CCCACTACCC ATGACCCGCT CCATGAGTCC 
TCCCCACTCC CTAGTGACTC CAAGCCTCCA AAGGCTGGCC CTCGAAAGGA GAGTGGGCGT 
CAGATTCGAC CAACTAAGAA AACCGAGGTG CCTGACTCTC AACTTCCTGC CCCCCCTGTT 
TTGCAACCCC AACCTACACC CACTGCTGAG AAGGACAGCA AGACAAGCGA GCAGTTACGG 
TATTGTGCAG GCATTATCCG GGAAATGTTT TCCAAAAAGC ATCAGGCCTA TGCGTGGCCA 
TTTTACAAGC CCGTTGATGT GGATGCCCTT GGGTTACATG ACTACCGTGA AATTATCAAG 
CATCCAATGG ACCTAGGCAC CATTAAGGTA AAGATGGATA ATTGGGACTA CAAGGACGCA 
AAGGAATTTG CATCTGATGT GCGGCTTATG TTCTCAAACT GTTACAAGTA CAATCCCCCT 
GATCATGAAG TTGTGATAAT GGCTCGCAAG TTGCAGGATG TTTTTGAAAT GCGCTATGCC 
AAAATGCCTG ATGAACCTGA GGAGGCGCCA GCTCCTGCAC CCTCTCCTGC TCCAGGGCCC 
CCAGCCCCAT CCATCAAAGG CCCTCCGCCA ACCTCTAGTG ACAGCAGCAG CGATAGCTCA 
TCTGATAGTG AAAGCAGTTC AGACAGTGAG GAGGAACGGG CACAAAGACT GGCAGAGCTT 
CAGGAACAGT TGAAAGCTGT ACATGAGCAG TTGGCAGCTC TATCTCAGCC ACAGCCGAAC 
AAACCAAAGA AGAAAGAGCG TGAAAAGCGG AAGGAAAAGC ACAAACGAAA AGAAGAGGTG 
GAGGAGCCTA GAAAGGGCCG TATCCGAGAG CCACCAGCCA AGAAACCCAA GAAAAGTGTT 
CAAGGGTCCG GAGGTACACC AAGCATCAAG AAAGAAGCAC CTCCCCCTGT GGCACGCCCT 
GCTCGTCCAG TGCCACCTCC GGCACCATGT GAGTCCTCTG AGGAAGAAAC GCAACGATGT 
CGGCCAATGT CATACGAAGA GAAACGCCAG CTAAGCTTGG ACATTAATAA GCTGCCAGGC 
GAAAAACTGG GCCGCGTCGT GCACATCATC CAGTCTCGCG AGCCTTCCCT GAAGAACTCC 
AATCCAGATG AGATTGAGAT AGACTTTGAG ACACTTAAAC CGTCCACACT GCGAGAGTTG 
GAGAGATATG TTACCTCCTG TTTGAGAAAG AAGCGAAAAC CACAGGATAA AATAGAAGCT 
CCCACATCTG GCACTGGTAA AGTAAAAGGC TATTCCTCTT CAGAGTCTGA GAGCTCAAGT 
GAGTCCAGCA CGTCAGACAG CGAGGAGTCC GACCCAGAAA CAGCCCCAAA TCAGAAGAAA 
AAGGGGCACT CGGGGAGAGA GTCTAGAAAG CATCATCACC CTATGCAACA GCCTCTTGCC 
GCACCACCCC CTGTCATGAA GCCTCCGTCT CCCACCTTAG CACCACCTTA CCCTCCGCCT 
TCCAGCCTGG ACTCCTCACA TCCTTCTCTC CACCACCCTC TCCACTCTGC GAATGTTTTT 
GATGCAGTCA TGCAGCTTCC TCCTGATCTG CCTCCTCACC TTACTGGTCA GACAGAGCAC 
TGCTCGCCCC CACACCTCAA CCAACATGCC CTGACAAGCC CTCCAGCCCT ACACAACGCA 
ATGCCCCAGC AGCCCTCCAG GCCATCCAAC CGTGCTGCTG CCCTTCCTCC AAAACCTGTT 
CAGCCTCCCT CTGTCTCTCC TCCTCTTCCT CCTCCTCCTC CGCAGCCCCA CCACCAGCCT 
TCTGTGCATA TCCATCATCA CCACCACCAT GCACAGCCCC CTCATGCTCT TCTGGAAGAT 
GATGGGCCTC CCTCTCCTCA CACAGGTCTC CCTTCCTACC TGCAACAGCT TCAGAAGCCA 
CAGCAACCTC CTACTCAGTC TCCTATTCAT TCACTTCTCA CATCTGTCAA GGTGCAGTCC 
CAAGCACCCA TGGCTGCTCC TCCCCAATCC ATGAGGCACC TCCAGCCTCT GGTTTACCCT 
GCTCCATCTT CCACTGCTAC CACAGCGCCT CCTCCTGCCT CTTCTCACAT ACACCAACTG 
CAGTCTTCCC CTGCAGTCCC TCAGCAACAG CCAGCTGGTC AGGCACCGCC ACCCCCCCAG 
CAGCAGCAGC ACCCGGCCCT GCAAGGCGCG CTCGTTTCCT CACATCCGCA GCATGTGCAG 
CACCAACAGG CCAAACAACA ACAAGTGATC CAGCATCATC ATCCTTCCCC ACGGCAGCAG 
AAGCAAGAGA CATATGCTGG AGGTCACTTG CGTGATGCTC CTTCCCCTCT CCTCCTGCAT 
TCGCCTCAGG TTCCTCCCTA TCCGGGTCTG GCTCACCCAC CTTCCCCACA GTCTGTGCAA 
CCTAAAAAGC AGGAGATGAG AGGAGCGTCG GTGCTGCAGC CTCAGCCGCT GGTCATGAAA 
GAAGATAAAC GTCATTCGCC TTCACTGCGC CCAGAGGGCT TCTCTCCAGG AATGCGCCCT 
GAACCTCAGA AAGTTCCGGA GGTTCTAAAA GGATCTAGTC ACATCCAGCC ACGACCAGAC 
TTGAAGCCAA TGGATGTTGG ACGTCCCATA CGGCCGACTG ACCAGAGCCT CCCACCTCAA 
GGGATGCCAG AAAAAGAGAA GCAAAAGCAA GAACCTAAGA CACCTGTGGC TCCTAAAAAG 
GACATAAAGA TTAAAAACAT GGGTTCGTGG GCTGGTCTAA TGCAGAAGCC ACCAGTAACC 
CCAACTTCTG CGGGAAAGTC AACAAGTGAC AGCTTTGAGC TGTTCCGGAG GCAGGCCCGC 
GAGAAAGAGG AGCGCGAGAG GGCCCTCAAA CTTCAGGCCG AACAAGCAGA GAGAATGCGC 
AGGGAGCAGG AGAGAATGAG GTCCCGGGAT GAAGATGACG CGCAGGAGCA GGCTAGGAAA 
GCCCACGAGG AGGCACGCCG GCGGCAGGAA CAACAGCAAC AGCAGCAACA ACAACAGCAG 
CAACAGCAAC ACGTGCAGAG CAACCTCCCC GCCGCTCCGT CACCTGCGCA GAGCTCTCAG 
CCAATCATGG ATCAGCGAGA AATGGCTAGA AAGCGGGAAC AGGAGAGGAG ACGAAGGCAG 
GCGATGGCGC CCTCTATAGA TATGAATTTT CAGAGTGAAC TGATGGAGAT ATTTGAACAG 
AACCTGTTCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809167.1
CDS	252..4346
Translation	MSTETGPGTRLRATSGMGDGVEGAQMSGQQLPAAPQPQPQNSSLLNPPPPDIARPSQAKRQTNQLQYLLKTVLKTLWKHQFAWPFQLPVDVVKLNLPDYHKIIKNPMDMGTIKKRLENHYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKISEMPQEETELAVVQSKGRGRGRKEQSEQDTSITPMRTRVLSGSLGDKSAVKPPPVTPVSKPATPTPPTVTRAPTPPQTRPPQQARPPAITQAPISFSPTISQDVVVPTTVAPTPVPQPLPNHPAVIHTAAQPAKTKKGVKRKADTTTPTTHDPLHESSPLPSDSKPPKAGPRKESGRQIRPTKKTEVPDSQLPAPPVLQPQPTPTAEKDSKTSEQLRYCAGIIREMFSKKHQAYAWPFYKPVDVDALGLHDYREIIKHPMDLGTIKVKMDNWDYKDAKEFASDVRLMFSNCYKYNPPDHEVVIMARKLQDVFEMRYAKMPDEPEEAPAPAPSPAPGPPAPSIKGPPPTSSDSSSDSSSDSESSSDSEEERAQRLAELQEQLKAVHEQLAALSQPQPNKPKKKEREKRKEKHKRKEEVEEPRKGRIREPPAKKPKKSVQGSGGTPSIKKEAPPPVARPARPVPPPAPCESSEEETQRCRPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQDKIEAPTSGTGKVKGYSSSESESSSESSTSDSEESDPETAPNQKKKGHSGRESRKHHHPMQQPLAAPPPVMKPPSPTLAPPYPPPSSLDSSHPSLHHPLHSANVFDAVMQLPPDLPPHLTGQTEHCSPPHLNQHALTSPPALHNAMPQQPSRPSNRAAALPPKPVQPPSVSPPLPPPPPQPHHQPSVHIHHHHHHAQPPHALLEDDGPPSPHTGLPSYLQQLQKPQQPPTQSPIHSLLTSVKVQSQAPMAAPPQSMRHLQPLVYPAPSSTATTAPPPASSHIHQLQSSPAVPQQQPAGQAPPPPQQQQHPALQGALVSSHPQHVQHQQAKQQQVIQHHHPSPRQQKQETYAGGHLRDAPSPLLLHSPQVPPYPGLAHPPSPQSVQPKKQEMRGASVLQPQPLVMKEDKRHSPSLRPEGFSPGMRPEPQKVPEVLKGSSHIQPRPDLKPMDVGRPIRPTDQSLPPQGMPEKEKQKQEPKTPVAPKKDIKIKNMGSWAGLMQKPPVTPTSAGKSTSDSFELFRRQAREKEERERALKLQAEQAERMRREQERMRSRDEDDAQEQARKAHEEARRRQEQQQQQQQQQQQQQHVQSNLPAAPSPAQSSQPIMDQREMARKREQERRRRQAMAPSIDMNFQSELMEIFEQNLFS

Target ORF information:

RefSeq Version	XM_012953713.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953713.2

ORF Insert Sequence:

ATGTCAACCG AGACTGGGCC TGGTACACGT CTGAGAGCCA CATCTGGAAT GGGTGACGGA 
GTAGAAGGGG CTCAGATGTC GGGCCAACAG CTGCCTGCCG CTCCCCAGCC CCAGCCACAG 
AACTCATCGC TGCTCAATCC TCCTCCTCCT GATATCGCAA GACCCAGCCA AGCAAAGCGC 
CAGACCAACC AGCTGCAGTA CTTACTTAAG ACAGTGCTCA AGACATTGTG GAAGCACCAG 
TTTGCATGGC CTTTCCAGCT ACCTGTTGAT GTGGTCAAGC TCAATCTGCC TGATTATCAT 
AAAATAATTA AAAACCCAAT GGACATGGGC ACCATTAAAA AGCGCCTCGA GAACCATTAT 
TACTGGAACG CCCAAGAATG TATCCAGGAC TTCAACACAA TGTTTACAAA CTGCTACATC 
TATAACAAGC CTGGAGATGA CATAGTGCTG ATGGCCGAAG CCCTTGAGAA GCTTTTTCTG 
CAGAAGATCT CAGAGATGCC CCAGGAAGAA ACCGAACTGG CAGTTGTGCA GAGCAAAGGC 
CGTGGCCGGG GAAGAAAGGA GCAAAGTGAG CAAGATACTT CAATCACCCC CATGAGAACC 
CGAGTCCTGT CTGGGTCACT AGGAGACAAG AGCGCCGTCA AACCTCCCCC TGTTACACCT 
GTGTCCAAGC CTGCAACCCC AACGCCCCCT ACTGTGACCA GGGCTCCCAC CCCTCCACAA 
ACCAGACCAC CCCAACAAGC TCGCCCTCCA GCTATCACTC AAGCCCCCAT CAGTTTCTCT 
CCCACCATCA GTCAGGATGT GGTGGTTCCA ACGACCGTAG CCCCAACACC AGTTCCTCAG 
CCACTTCCAA ATCATCCTGC AGTCATCCAC ACTGCTGCCC AGCCTGCAAA GACAAAGAAA 
GGTGTGAAGA GGAAAGCAGA CACCACAACC CCCACTACCC ATGACCCGCT CCATGAGTCC 
TCCCCACTCC CTAGTGACTC CAAGCCTCCA AAGGCTGGCC CTCGAAAGGA GAGTGGGCGT 
CAGATTCGAC CAACTAAGAA AACCGAGGTG CCTGACTCTC AACTTCCTGC CCCCCCTGTT 
TTGCAACCCC AACCTACACC CACTGCTGAG AAGGACAGCA AGACAAGCGA GCAGTTACGG 
TATTGTGCAG GCATTATCCG GGAAATGTTT TCCAAAAAGC ATCAGGCCTA TGCGTGGCCA 
TTTTACAAGC CCGTTGATGT GGATGCCCTT GGGTTACATG ACTACCGTGA AATTATCAAG 
CATCCAATGG ACCTAGGCAC CATTAAGGTA AAGATGGATA ATTGGGACTA CAAGGACGCA 
AAGGAATTTG CATCTGATGT GCGGCTTATG TTCTCAAACT GTTACAAGTA CAATCCCCCT 
GATCATGAAG TTGTGATAAT GGCTCGCAAG TTGCAGGATG TTTTTGAAAT GCGCTATGCC 
AAAATGCCTG ATGAACCTGA GGAGGCGCCA GCTCCTGCAC CCTCTCCTGC TCCAGGGCCC 
CCAGCCCCAT CCATCAAAGG CCCTCCGCCA ACCTCTAGTG ACAGCAGCAG CGATAGCTCA 
TCTGATAGTG AAAGCAGTTC AGACAGTGAG GAGGAACGGG CACAAAGACT GGCAGAGCTT 
CAGGAACAGT TGAAAGCTGT ACATGAGCAG TTGGCAGCTC TATCTCAGCC ACAGCCGAAC 
AAACCAAAGA AGAAAGAGCG TGAAAAGCGG AAGGAAAAGC ACAAACGAAA AGAAGAGGTG 
GAGGAGCCTA GAAAGGGCCG TATCCGAGAG CCACCAGCCA AGAAACCCAA GAAAAGTGTT 
CAAGGGTCCG GAGGTACACC AAGCATCAAG AAAGAAGCAC CTCCCCCTGT GGCACGCCCT 
GCTCGTCCAG TGCCACCTCC GGCACCATGT GAGTCCTCTG AGGAAGAAAC GCAACGATGT 
CGGCCAATGT CATACGAAGA GAAACGCCAG CTAAGCTTGG ACATTAATAA GCTGCCAGGC 
GAAAAACTGG GCCGCGTCGT GCACATCATC CAGTCTCGCG AGCCTTCCCT GAAGAACTCC 
AATCCAGATG AGATTGAGAT AGACTTTGAG ACACTTAAAC CGTCCACACT GCGAGAGTTG 
GAGAGATATG TTACCTCCTG TTTGAGAAAG AAGCGAAAAC CACAGGATAA AATAGAAGCT 
CCCACATCTG GCACTGGTAA AGTAAAAGGC TATTCCTCTT CAGAGTCTGA GAGCTCAAGT 
GAGTCCAGCA CGTCAGACAG CGAGGAGTCC GACCCAGAAA CAGCCCCAAA TCAGAAGAAA 
AAGGGGCACT CGGGGAGAGA GTCTAGAAAG CATCATCACC CTATGCAACA GCCTCTTGCC 
GCACCACCCC CTGTCATGAA GCCTCCGTCT CCCACCTTAG CACCACCTTA CCCTCCGCCT 
TCCAGCCTGG ACTCCTCACA TCCTTCTCTC CACCACCCTC TCCACTCTGC GAATGTTTTT 
GATGCAGTCA TGCAGCTTCC TCCTGATCTG CCTCCTCACC TTACTGGTCA GACAGAGCAC 
TGCTCGCCCC CACACCTCAA CCAACATGCC CTGACAAGCC CTCCAGCCCT ACACAACGCA 
ATGCCCCAGC AGCCCTCCAG GCCATCCAAC CGTGCTGCTG CCCTTCCTCC AAAACCTGTT 
CAGCCTCCCT CTGTCTCTCC TCCTCTTCCT CCTCCTCCTC CGCAGCCCCA CCACCAGCCT 
TCTGTGCATA TCCATCATCA CCACCACCAT GCACAGCCCC CTCATGCTCT TCTGGAAGAT 
GATGGGCCTC CCTCTCCTCA CACAGGTCTC CCTTCCTACC TGCAACAGCT TCAGAAGCCA 
CAGCAACCTC CTACTCAGTC TCCTATTCAT TCACTTCTCA CATCTGTCAA GGTGCAGTCC 
CAAGCACCCA TGGCTGCTCC TCCCCAATCC ATGAGGCACC TCCAGCCTCT GGTTTACCCT 
GCTCCATCTT CCACTGCTAC CACAGCGCCT CCTCCTGCCT CTTCTCACAT ACACCAACTG 
CAGTCTTCCC CTGCAGTCCC TCAGCAACAG CCAGCTGGTC AGGCACCGCC ACCCCCCCAG 
CAGCAGCAGC ACCCGGCCCT GCAAGGCGCG CTCGTTTCCT CACATCCGCA GCATGTGCAG 
CACCAACAGG CCAAACAACA ACAAGTGATC CAGCATCATC ATCCTTCCCC ACGGCAGCAG 
AAGCAAGAGA CATATGCTGG AGGTCACTTG CGTGATGCTC CTTCCCCTCT CCTCCTGCAT 
TCGCCTCAGG TTCCTCCCTA TCCGGGTCTG GCTCACCCAC CTTCCCCACA GTCTGTGCAA 
CCTAAAAAGC AGGAGATGAG AGGAGCGTCG GTGCTGCAGC CTCAGCCGCT GGTCATGAAA 
GAAGATAAAC GTCATTCGCC TTCACTGCGC CCAGAGGGCT TCTCTCCAGG AATGCGCCCT 
GAACCTCAGA AAGTTCCGGA GGTTCTAAAA GGATCTAGTC ACATCCAGCC ACGACCAGAC 
TTGAAGCCAA TGGATGTTGG ACGTCCCATA CGGCCGACTG ACCAGAGCCT CCCACCTCAA 
GGGATGCCAG AAAAAGAGAA GCAAAAGCAA GAACCTAAGA CACCTGTGGC TCCTAAAAAG 
GACATAAAGA TTAAAAACAT GGGTTCGTGG GCTGGTCTAA TGCAGAAGCC ACCAGTAACC 
CCAACTTCTG CGGGAAAGTC AACAAGTGAC AGCTTTGAGC TGTTCCGGAG GCAGGCCCGC 
GAGAAAGAGG AGCGCGAGAG GGCCCTCAAA CTTCAGGCCG AACAAGCAGA GAGAATGCGC 
AGGGAGCAGG AGAGAATGAG GTCCCGGGAT GAAGATGACG CGCAGGAGCA GGCTAGGAAA 
GCCCACGAGG AGGCACGCCG GCGGCAGGAA CAACAGCAAC AGCAGCAACA ACAACAGCAG 
CAACAGCAAC ACGTGCAGAG CAACCTCCCC GCCGCTCCGT CACCTGCGCA GAGCTCTCAG 
CCAATCATGG ATCAGCGAGA AATGGCTAGA AAGCGGGAAC AGGAGAGGAG ACGAAGGCAG 
GCGATGGCGC CCTCTATAGA TATGAATTTT CAGAGTGAAC TGATGGAGAT ATTTGAACAG 
AACCTGTTCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brd4 ( XM_012953714.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809168.1 Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa32766
Clone ID Related Accession (Same CDS sequence)	XM_012953714.2 , XM_012953713.2 , XM_012953714.1 , XM_012953713.3
Accession Version	XM_012953714.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACCG AGACTGGGCC TGGTACACGT CTGAGAGCCA CATCTGGAAT GGGTGACGGA 
GTAGAAGGGG CTCAGATGTC GGGCCAACAG CTGCCTGCCG CTCCCCAGCC CCAGCCACAG 
AACTCATCGC TGCTCAATCC TCCTCCTCCT GATATCGCAA GACCCAGCCA AGCAAAGCGC 
CAGACCAACC AGCTGCAGTA CTTACTTAAG ACAGTGCTCA AGACATTGTG GAAGCACCAG 
TTTGCATGGC CTTTCCAGCT ACCTGTTGAT GTGGTCAAGC TCAATCTGCC TGATTATCAT 
AAAATAATTA AAAACCCAAT GGACATGGGC ACCATTAAAA AGCGCCTCGA GAACCATTAT 
TACTGGAACG CCCAAGAATG TATCCAGGAC TTCAACACAA TGTTTACAAA CTGCTACATC 
TATAACAAGC CTGGAGATGA CATAGTGCTG ATGGCCGAAG CCCTTGAGAA GCTTTTTCTG 
CAGAAGATCT CAGAGATGCC CCAGGAAGAA ACCGAACTGG CAGTTGTGCA GAGCAAAGGC 
CGTGGCCGGG GAAGAAAGGA GCAAAGTGAG CAAGATACTT CAATCACCCC CATGAGAACC 
CGAGTCCTGT CTGGGTCACT AGGAGACAAG AGCGCCGTCA AACCTCCCCC TGTTACACCT 
GTGTCCAAGC CTGCAACCCC AACGCCCCCT ACTGTGACCA GGGCTCCCAC CCCTCCACAA 
ACCAGACCAC CCCAACAAGC TCGCCCTCCA GCTATCACTC AAGCCCCCAT CAGTTTCTCT 
CCCACCATCA GTCAGGATGT GGTGGTTCCA ACGACCGTAG CCCCAACACC AGTTCCTCAG 
CCACTTCCAA ATCATCCTGC AGTCATCCAC ACTGCTGCCC AGCCTGCAAA GACAAAGAAA 
GGTGTGAAGA GGAAAGCAGA CACCACAACC CCCACTACCC ATGACCCGCT CCATGAGTCC 
TCCCCACTCC CTAGTGACTC CAAGCCTCCA AAGGCTGGCC CTCGAAAGGA GAGTGGGCGT 
CAGATTCGAC CAACTAAGAA AACCGAGGTG CCTGACTCTC AACTTCCTGC CCCCCCTGTT 
TTGCAACCCC AACCTACACC CACTGCTGAG AAGGACAGCA AGACAAGCGA GCAGTTACGG 
TATTGTGCAG GCATTATCCG GGAAATGTTT TCCAAAAAGC ATCAGGCCTA TGCGTGGCCA 
TTTTACAAGC CCGTTGATGT GGATGCCCTT GGGTTACATG ACTACCGTGA AATTATCAAG 
CATCCAATGG ACCTAGGCAC CATTAAGGTA AAGATGGATA ATTGGGACTA CAAGGACGCA 
AAGGAATTTG CATCTGATGT GCGGCTTATG TTCTCAAACT GTTACAAGTA CAATCCCCCT 
GATCATGAAG TTGTGATAAT GGCTCGCAAG TTGCAGGATG TTTTTGAAAT GCGCTATGCC 
AAAATGCCTG ATGAACCTGA GGAGGCGCCA GCTCCTGCAC CCTCTCCTGC TCCAGGGCCC 
CCAGCCCCAT CCATCAAAGG CCCTCCGCCA ACCTCTAGTG ACAGCAGCAG CGATAGCTCA 
TCTGATAGTG AAAGCAGTTC AGACAGTGAG GAGGAACGGG CACAAAGACT GGCAGAGCTT 
CAGGAACAGT TGAAAGCTGT ACATGAGCAG TTGGCAGCTC TATCTCAGCC ACAGCCGAAC 
AAACCAAAGA AGAAAGAGCG TGAAAAGCGG AAGGAAAAGC ACAAACGAAA AGAAGAGGTG 
GAGGAGCCTA GAAAGGGCCG TATCCGAGAG CCACCAGCCA AGAAACCCAA GAAAAGTGTT 
CAAGGGTCCG GAGGTACACC AAGCATCAAG AAAGAAGCAC CTCCCCCTGT GGCACGCCCT 
GCTCGTCCAG TGCCACCTCC GGCACCATGT GAGTCCTCTG AGGAAGAAAC GCAACGATGT 
CGGCCAATGT CATACGAAGA GAAACGCCAG CTAAGCTTGG ACATTAATAA GCTGCCAGGC 
GAAAAACTGG GCCGCGTCGT GCACATCATC CAGTCTCGCG AGCCTTCCCT GAAGAACTCC 
AATCCAGATG AGATTGAGAT AGACTTTGAG ACACTTAAAC CGTCCACACT GCGAGAGTTG 
GAGAGATATG TTACCTCCTG TTTGAGAAAG AAGCGAAAAC CACAGGATAA AATAGAAGCT 
CCCACATCTG GCACTGGTAA AGTAAAAGGC TATTCCTCTT CAGAGTCTGA GAGCTCAAGT 
GAGTCCAGCA CGTCAGACAG CGAGGAGTCC GACCCAGAAA CAGCCCCAAA TCAGAAGAAA 
AAGGGGCACT CGGGGAGAGA GTCTAGAAAG CATCATCACC CTATGCAACA GCCTCTTGCC 
GCACCACCCC CTGTCATGAA GCCTCCGTCT CCCACCTTAG CACCACCTTA CCCTCCGCCT 
TCCAGCCTGG ACTCCTCACA TCCTTCTCTC CACCACCCTC TCCACTCTGC GAATGTTTTT 
GATGCAGTCA TGCAGCTTCC TCCTGATCTG CCTCCTCACC TTACTGGTCA GACAGAGCAC 
TGCTCGCCCC CACACCTCAA CCAACATGCC CTGACAAGCC CTCCAGCCCT ACACAACGCA 
ATGCCCCAGC AGCCCTCCAG GCCATCCAAC CGTGCTGCTG CCCTTCCTCC AAAACCTGTT 
CAGCCTCCCT CTGTCTCTCC TCCTCTTCCT CCTCCTCCTC CGCAGCCCCA CCACCAGCCT 
TCTGTGCATA TCCATCATCA CCACCACCAT GCACAGCCCC CTCATGCTCT TCTGGAAGAT 
GATGGGCCTC CCTCTCCTCA CACAGGTCTC CCTTCCTACC TGCAACAGCT TCAGAAGCCA 
CAGCAACCTC CTACTCAGTC TCCTATTCAT TCACTTCTCA CATCTGTCAA GGTGCAGTCC 
CAAGCACCCA TGGCTGCTCC TCCCCAATCC ATGAGGCACC TCCAGCCTCT GGTTTACCCT 
GCTCCATCTT CCACTGCTAC CACAGCGCCT CCTCCTGCCT CTTCTCACAT ACACCAACTG 
CAGTCTTCCC CTGCAGTCCC TCAGCAACAG CCAGCTGGTC AGGCACCGCC ACCCCCCCAG 
CAGCAGCAGC ACCCGGCCCT GCAAGGCGCG CTCGTTTCCT CACATCCGCA GCATGTGCAG 
CACCAACAGG CCAAACAACA ACAAGTGATC CAGCATCATC ATCCTTCCCC ACGGCAGCAG 
AAGCAAGAGA CATATGCTGG AGGTCACTTG CGTGATGCTC CTTCCCCTCT CCTCCTGCAT 
TCGCCTCAGG TTCCTCCCTA TCCGGGTCTG GCTCACCCAC CTTCCCCACA GTCTGTGCAA 
CCTAAAAAGC AGGAGATGAG AGGAGCGTCG GTGCTGCAGC CTCAGCCGCT GGTCATGAAA 
GAAGATAAAC GTCATTCGCC TTCACTGCGC CCAGAGGGCT TCTCTCCAGG AATGCGCCCT 
GAACCTCAGA AAGTTCCGGA GGTTCTAAAA GGATCTAGTC ACATCCAGCC ACGACCAGAC 
TTGAAGCCAA TGGATGTTGG ACGTCCCATA CGGCCGACTG ACCAGAGCCT CCCACCTCAA 
GGGATGCCAG AAAAAGAGAA GCAAAAGCAA GAACCTAAGA CACCTGTGGC TCCTAAAAAG 
GACATAAAGA TTAAAAACAT GGGTTCGTGG GCTGGTCTAA TGCAGAAGCC ACCAGTAACC 
CCAACTTCTG CGGGAAAGTC AACAAGTGAC AGCTTTGAGC TGTTCCGGAG GCAGGCCCGC 
GAGAAAGAGG AGCGCGAGAG GGCCCTCAAA CTTCAGGCCG AACAAGCAGA GAGAATGCGC 
AGGGAGCAGG AGAGAATGAG GTCCCGGGAT GAAGATGACG CGCAGGAGCA GGCTAGGAAA 
GCCCACGAGG AGGCACGCCG GCGGCAGGAA CAACAGCAAC AGCAGCAACA ACAACAGCAG 
CAACAGCAAC ACGTGCAGAG CAACCTCCCC GCCGCTCCGT CACCTGCGCA GAGCTCTCAG 
CCAATCATGG ATCAGCGAGA AATGGCTAGA AAGCGGGAAC AGGAGAGGAG ACGAAGGCAG 
GCGATGGCGC CCTCTATAGA TATGAATTTT CAGAGTGAAC TGATGGAGAT ATTTGAACAG 
AACCTGTTCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809168.1
CDS	261..4355
Translation	MSTETGPGTRLRATSGMGDGVEGAQMSGQQLPAAPQPQPQNSSLLNPPPPDIARPSQAKRQTNQLQYLLKTVLKTLWKHQFAWPFQLPVDVVKLNLPDYHKIIKNPMDMGTIKKRLENHYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKISEMPQEETELAVVQSKGRGRGRKEQSEQDTSITPMRTRVLSGSLGDKSAVKPPPVTPVSKPATPTPPTVTRAPTPPQTRPPQQARPPAITQAPISFSPTISQDVVVPTTVAPTPVPQPLPNHPAVIHTAAQPAKTKKGVKRKADTTTPTTHDPLHESSPLPSDSKPPKAGPRKESGRQIRPTKKTEVPDSQLPAPPVLQPQPTPTAEKDSKTSEQLRYCAGIIREMFSKKHQAYAWPFYKPVDVDALGLHDYREIIKHPMDLGTIKVKMDNWDYKDAKEFASDVRLMFSNCYKYNPPDHEVVIMARKLQDVFEMRYAKMPDEPEEAPAPAPSPAPGPPAPSIKGPPPTSSDSSSDSSSDSESSSDSEEERAQRLAELQEQLKAVHEQLAALSQPQPNKPKKKEREKRKEKHKRKEEVEEPRKGRIREPPAKKPKKSVQGSGGTPSIKKEAPPPVARPARPVPPPAPCESSEEETQRCRPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQDKIEAPTSGTGKVKGYSSSESESSSESSTSDSEESDPETAPNQKKKGHSGRESRKHHHPMQQPLAAPPPVMKPPSPTLAPPYPPPSSLDSSHPSLHHPLHSANVFDAVMQLPPDLPPHLTGQTEHCSPPHLNQHALTSPPALHNAMPQQPSRPSNRAAALPPKPVQPPSVSPPLPPPPPQPHHQPSVHIHHHHHHAQPPHALLEDDGPPSPHTGLPSYLQQLQKPQQPPTQSPIHSLLTSVKVQSQAPMAAPPQSMRHLQPLVYPAPSSTATTAPPPASSHIHQLQSSPAVPQQQPAGQAPPPPQQQQHPALQGALVSSHPQHVQHQQAKQQQVIQHHHPSPRQQKQETYAGGHLRDAPSPLLLHSPQVPPYPGLAHPPSPQSVQPKKQEMRGASVLQPQPLVMKEDKRHSPSLRPEGFSPGMRPEPQKVPEVLKGSSHIQPRPDLKPMDVGRPIRPTDQSLPPQGMPEKEKQKQEPKTPVAPKKDIKIKNMGSWAGLMQKPPVTPTSAGKSTSDSFELFRRQAREKEERERALKLQAEQAERMRREQERMRSRDEDDAQEQARKAHEEARRRQEQQQQQQQQQQQQQHVQSNLPAAPSPAQSSQPIMDQREMARKREQERRRRQAMAPSIDMNFQSELMEIFEQNLFS

Target ORF information:

RefSeq Version	XM_012953714.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953714.1

ORF Insert Sequence:

ATGTCAACCG AGACTGGGCC TGGTACACGT CTGAGAGCCA CATCTGGAAT GGGTGACGGA 
GTAGAAGGGG CTCAGATGTC GGGCCAACAG CTGCCTGCCG CTCCCCAGCC CCAGCCACAG 
AACTCATCGC TGCTCAATCC TCCTCCTCCT GATATCGCAA GACCCAGCCA AGCAAAGCGC 
CAGACCAACC AGCTGCAGTA CTTACTTAAG ACAGTGCTCA AGACATTGTG GAAGCACCAG 
TTTGCATGGC CTTTCCAGCT ACCTGTTGAT GTGGTCAAGC TCAATCTGCC TGATTATCAT 
AAAATAATTA AAAACCCAAT GGACATGGGC ACCATTAAAA AGCGCCTCGA GAACCATTAT 
TACTGGAACG CCCAAGAATG TATCCAGGAC TTCAACACAA TGTTTACAAA CTGCTACATC 
TATAACAAGC CTGGAGATGA CATAGTGCTG ATGGCCGAAG CCCTTGAGAA GCTTTTTCTG 
CAGAAGATCT CAGAGATGCC CCAGGAAGAA ACCGAACTGG CAGTTGTGCA GAGCAAAGGC 
CGTGGCCGGG GAAGAAAGGA GCAAAGTGAG CAAGATACTT CAATCACCCC CATGAGAACC 
CGAGTCCTGT CTGGGTCACT AGGAGACAAG AGCGCCGTCA AACCTCCCCC TGTTACACCT 
GTGTCCAAGC CTGCAACCCC AACGCCCCCT ACTGTGACCA GGGCTCCCAC CCCTCCACAA 
ACCAGACCAC CCCAACAAGC TCGCCCTCCA GCTATCACTC AAGCCCCCAT CAGTTTCTCT 
CCCACCATCA GTCAGGATGT GGTGGTTCCA ACGACCGTAG CCCCAACACC AGTTCCTCAG 
CCACTTCCAA ATCATCCTGC AGTCATCCAC ACTGCTGCCC AGCCTGCAAA GACAAAGAAA 
GGTGTGAAGA GGAAAGCAGA CACCACAACC CCCACTACCC ATGACCCGCT CCATGAGTCC 
TCCCCACTCC CTAGTGACTC CAAGCCTCCA AAGGCTGGCC CTCGAAAGGA GAGTGGGCGT 
CAGATTCGAC CAACTAAGAA AACCGAGGTG CCTGACTCTC AACTTCCTGC CCCCCCTGTT 
TTGCAACCCC AACCTACACC CACTGCTGAG AAGGACAGCA AGACAAGCGA GCAGTTACGG 
TATTGTGCAG GCATTATCCG GGAAATGTTT TCCAAAAAGC ATCAGGCCTA TGCGTGGCCA 
TTTTACAAGC CCGTTGATGT GGATGCCCTT GGGTTACATG ACTACCGTGA AATTATCAAG 
CATCCAATGG ACCTAGGCAC CATTAAGGTA AAGATGGATA ATTGGGACTA CAAGGACGCA 
AAGGAATTTG CATCTGATGT GCGGCTTATG TTCTCAAACT GTTACAAGTA CAATCCCCCT 
GATCATGAAG TTGTGATAAT GGCTCGCAAG TTGCAGGATG TTTTTGAAAT GCGCTATGCC 
AAAATGCCTG ATGAACCTGA GGAGGCGCCA GCTCCTGCAC CCTCTCCTGC TCCAGGGCCC 
CCAGCCCCAT CCATCAAAGG CCCTCCGCCA ACCTCTAGTG ACAGCAGCAG CGATAGCTCA 
TCTGATAGTG AAAGCAGTTC AGACAGTGAG GAGGAACGGG CACAAAGACT GGCAGAGCTT 
CAGGAACAGT TGAAAGCTGT ACATGAGCAG TTGGCAGCTC TATCTCAGCC ACAGCCGAAC 
AAACCAAAGA AGAAAGAGCG TGAAAAGCGG AAGGAAAAGC ACAAACGAAA AGAAGAGGTG 
GAGGAGCCTA GAAAGGGCCG TATCCGAGAG CCACCAGCCA AGAAACCCAA GAAAAGTGTT 
CAAGGGTCCG GAGGTACACC AAGCATCAAG AAAGAAGCAC CTCCCCCTGT GGCACGCCCT 
GCTCGTCCAG TGCCACCTCC GGCACCATGT GAGTCCTCTG AGGAAGAAAC GCAACGATGT 
CGGCCAATGT CATACGAAGA GAAACGCCAG CTAAGCTTGG ACATTAATAA GCTGCCAGGC 
GAAAAACTGG GCCGCGTCGT GCACATCATC CAGTCTCGCG AGCCTTCCCT GAAGAACTCC 
AATCCAGATG AGATTGAGAT AGACTTTGAG ACACTTAAAC CGTCCACACT GCGAGAGTTG 
GAGAGATATG TTACCTCCTG TTTGAGAAAG AAGCGAAAAC CACAGGATAA AATAGAAGCT 
CCCACATCTG GCACTGGTAA AGTAAAAGGC TATTCCTCTT CAGAGTCTGA GAGCTCAAGT 
GAGTCCAGCA CGTCAGACAG CGAGGAGTCC GACCCAGAAA CAGCCCCAAA TCAGAAGAAA 
AAGGGGCACT CGGGGAGAGA GTCTAGAAAG CATCATCACC CTATGCAACA GCCTCTTGCC 
GCACCACCCC CTGTCATGAA GCCTCCGTCT CCCACCTTAG CACCACCTTA CCCTCCGCCT 
TCCAGCCTGG ACTCCTCACA TCCTTCTCTC CACCACCCTC TCCACTCTGC GAATGTTTTT 
GATGCAGTCA TGCAGCTTCC TCCTGATCTG CCTCCTCACC TTACTGGTCA GACAGAGCAC 
TGCTCGCCCC CACACCTCAA CCAACATGCC CTGACAAGCC CTCCAGCCCT ACACAACGCA 
ATGCCCCAGC AGCCCTCCAG GCCATCCAAC CGTGCTGCTG CCCTTCCTCC AAAACCTGTT 
CAGCCTCCCT CTGTCTCTCC TCCTCTTCCT CCTCCTCCTC CGCAGCCCCA CCACCAGCCT 
TCTGTGCATA TCCATCATCA CCACCACCAT GCACAGCCCC CTCATGCTCT TCTGGAAGAT 
GATGGGCCTC CCTCTCCTCA CACAGGTCTC CCTTCCTACC TGCAACAGCT TCAGAAGCCA 
CAGCAACCTC CTACTCAGTC TCCTATTCAT TCACTTCTCA CATCTGTCAA GGTGCAGTCC 
CAAGCACCCA TGGCTGCTCC TCCCCAATCC ATGAGGCACC TCCAGCCTCT GGTTTACCCT 
GCTCCATCTT CCACTGCTAC CACAGCGCCT CCTCCTGCCT CTTCTCACAT ACACCAACTG 
CAGTCTTCCC CTGCAGTCCC TCAGCAACAG CCAGCTGGTC AGGCACCGCC ACCCCCCCAG 
CAGCAGCAGC ACCCGGCCCT GCAAGGCGCG CTCGTTTCCT CACATCCGCA GCATGTGCAG 
CACCAACAGG CCAAACAACA ACAAGTGATC CAGCATCATC ATCCTTCCCC ACGGCAGCAG 
AAGCAAGAGA CATATGCTGG AGGTCACTTG CGTGATGCTC CTTCCCCTCT CCTCCTGCAT 
TCGCCTCAGG TTCCTCCCTA TCCGGGTCTG GCTCACCCAC CTTCCCCACA GTCTGTGCAA 
CCTAAAAAGC AGGAGATGAG AGGAGCGTCG GTGCTGCAGC CTCAGCCGCT GGTCATGAAA 
GAAGATAAAC GTCATTCGCC TTCACTGCGC CCAGAGGGCT TCTCTCCAGG AATGCGCCCT 
GAACCTCAGA AAGTTCCGGA GGTTCTAAAA GGATCTAGTC ACATCCAGCC ACGACCAGAC 
TTGAAGCCAA TGGATGTTGG ACGTCCCATA CGGCCGACTG ACCAGAGCCT CCCACCTCAA 
GGGATGCCAG AAAAAGAGAA GCAAAAGCAA GAACCTAAGA CACCTGTGGC TCCTAAAAAG 
GACATAAAGA TTAAAAACAT GGGTTCGTGG GCTGGTCTAA TGCAGAAGCC ACCAGTAACC 
CCAACTTCTG CGGGAAAGTC AACAAGTGAC AGCTTTGAGC TGTTCCGGAG GCAGGCCCGC 
GAGAAAGAGG AGCGCGAGAG GGCCCTCAAA CTTCAGGCCG AACAAGCAGA GAGAATGCGC 
AGGGAGCAGG AGAGAATGAG GTCCCGGGAT GAAGATGACG CGCAGGAGCA GGCTAGGAAA 
GCCCACGAGG AGGCACGCCG GCGGCAGGAA CAACAGCAAC AGCAGCAACA ACAACAGCAG 
CAACAGCAAC ACGTGCAGAG CAACCTCCCC GCCGCTCCGT CACCTGCGCA GAGCTCTCAG 
CCAATCATGG ATCAGCGAGA AATGGCTAGA AAGCGGGAAC AGGAGAGGAG ACGAAGGCAG 
GCGATGGCGC CCTCTATAGA TATGAATTTT CAGAGTGAAC TGATGGAGAT ATTTGAACAG 
AACCTGTTCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brd4 ( XM_012953714.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809168.1 Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X3, mRNA.

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CloneID	OXa32766
Clone ID Related Accession (Same CDS sequence)	XM_012953714.2 , XM_012953713.2 , XM_012953714.1 , XM_012953713.3
Accession Version	XM_012953714.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012953714.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACCG AGACTGGGCC TGGTACACGT CTGAGAGCCA CATCTGGAAT GGGTGACGGA 
GTAGAAGGGG CTCAGATGTC GGGCCAACAG CTGCCTGCCG CTCCCCAGCC CCAGCCACAG 
AACTCATCGC TGCTCAATCC TCCTCCTCCT GATATCGCAA GACCCAGCCA AGCAAAGCGC 
CAGACCAACC AGCTGCAGTA CTTACTTAAG ACAGTGCTCA AGACATTGTG GAAGCACCAG 
TTTGCATGGC CTTTCCAGCT ACCTGTTGAT GTGGTCAAGC TCAATCTGCC TGATTATCAT 
AAAATAATTA AAAACCCAAT GGACATGGGC ACCATTAAAA AGCGCCTCGA GAACCATTAT 
TACTGGAACG CCCAAGAATG TATCCAGGAC TTCAACACAA TGTTTACAAA CTGCTACATC 
TATAACAAGC CTGGAGATGA CATAGTGCTG ATGGCCGAAG CCCTTGAGAA GCTTTTTCTG 
CAGAAGATCT CAGAGATGCC CCAGGAAGAA ACCGAACTGG CAGTTGTGCA GAGCAAAGGC 
CGTGGCCGGG GAAGAAAGGA GCAAAGTGAG CAAGATACTT CAATCACCCC CATGAGAACC 
CGAGTCCTGT CTGGGTCACT AGGAGACAAG AGCGCCGTCA AACCTCCCCC TGTTACACCT 
GTGTCCAAGC CTGCAACCCC AACGCCCCCT ACTGTGACCA GGGCTCCCAC CCCTCCACAA 
ACCAGACCAC CCCAACAAGC TCGCCCTCCA GCTATCACTC AAGCCCCCAT CAGTTTCTCT 
CCCACCATCA GTCAGGATGT GGTGGTTCCA ACGACCGTAG CCCCAACACC AGTTCCTCAG 
CCACTTCCAA ATCATCCTGC AGTCATCCAC ACTGCTGCCC AGCCTGCAAA GACAAAGAAA 
GGTGTGAAGA GGAAAGCAGA CACCACAACC CCCACTACCC ATGACCCGCT CCATGAGTCC 
TCCCCACTCC CTAGTGACTC CAAGCCTCCA AAGGCTGGCC CTCGAAAGGA GAGTGGGCGT 
CAGATTCGAC CAACTAAGAA AACCGAGGTG CCTGACTCTC AACTTCCTGC CCCCCCTGTT 
TTGCAACCCC AACCTACACC CACTGCTGAG AAGGACAGCA AGACAAGCGA GCAGTTACGG 
TATTGTGCAG GCATTATCCG GGAAATGTTT TCCAAAAAGC ATCAGGCCTA TGCGTGGCCA 
TTTTACAAGC CCGTTGATGT GGATGCCCTT GGGTTACATG ACTACCGTGA AATTATCAAG 
CATCCAATGG ACCTAGGCAC CATTAAGGTA AAGATGGATA ATTGGGACTA CAAGGACGCA 
AAGGAATTTG CATCTGATGT GCGGCTTATG TTCTCAAACT GTTACAAGTA CAATCCCCCT 
GATCATGAAG TTGTGATAAT GGCTCGCAAG TTGCAGGATG TTTTTGAAAT GCGCTATGCC 
AAAATGCCTG ATGAACCTGA GGAGGCGCCA GCTCCTGCAC CCTCTCCTGC TCCAGGGCCC 
CCAGCCCCAT CCATCAAAGG CCCTCCGCCA ACCTCTAGTG ACAGCAGCAG CGATAGCTCA 
TCTGATAGTG AAAGCAGTTC AGACAGTGAG GAGGAACGGG CACAAAGACT GGCAGAGCTT 
CAGGAACAGT TGAAAGCTGT ACATGAGCAG TTGGCAGCTC TATCTCAGCC ACAGCCGAAC 
AAACCAAAGA AGAAAGAGCG TGAAAAGCGG AAGGAAAAGC ACAAACGAAA AGAAGAGGTG 
GAGGAGCCTA GAAAGGGCCG TATCCGAGAG CCACCAGCCA AGAAACCCAA GAAAAGTGTT 
CAAGGGTCCG GAGGTACACC AAGCATCAAG AAAGAAGCAC CTCCCCCTGT GGCACGCCCT 
GCTCGTCCAG TGCCACCTCC GGCACCATGT GAGTCCTCTG AGGAAGAAAC GCAACGATGT 
CGGCCAATGT CATACGAAGA GAAACGCCAG CTAAGCTTGG ACATTAATAA GCTGCCAGGC 
GAAAAACTGG GCCGCGTCGT GCACATCATC CAGTCTCGCG AGCCTTCCCT GAAGAACTCC 
AATCCAGATG AGATTGAGAT AGACTTTGAG ACACTTAAAC CGTCCACACT GCGAGAGTTG 
GAGAGATATG TTACCTCCTG TTTGAGAAAG AAGCGAAAAC CACAGGATAA AATAGAAGCT 
CCCACATCTG GCACTGGTAA AGTAAAAGGC TATTCCTCTT CAGAGTCTGA GAGCTCAAGT 
GAGTCCAGCA CGTCAGACAG CGAGGAGTCC GACCCAGAAA CAGCCCCAAA TCAGAAGAAA 
AAGGGGCACT CGGGGAGAGA GTCTAGAAAG CATCATCACC CTATGCAACA GCCTCTTGCC 
GCACCACCCC CTGTCATGAA GCCTCCGTCT CCCACCTTAG CACCACCTTA CCCTCCGCCT 
TCCAGCCTGG ACTCCTCACA TCCTTCTCTC CACCACCCTC TCCACTCTGC GAATGTTTTT 
GATGCAGTCA TGCAGCTTCC TCCTGATCTG CCTCCTCACC TTACTGGTCA GACAGAGCAC 
TGCTCGCCCC CACACCTCAA CCAACATGCC CTGACAAGCC CTCCAGCCCT ACACAACGCA 
ATGCCCCAGC AGCCCTCCAG GCCATCCAAC CGTGCTGCTG CCCTTCCTCC AAAACCTGTT 
CAGCCTCCCT CTGTCTCTCC TCCTCTTCCT CCTCCTCCTC CGCAGCCCCA CCACCAGCCT 
TCTGTGCATA TCCATCATCA CCACCACCAT GCACAGCCCC CTCATGCTCT TCTGGAAGAT 
GATGGGCCTC CCTCTCCTCA CACAGGTCTC CCTTCCTACC TGCAACAGCT TCAGAAGCCA 
CAGCAACCTC CTACTCAGTC TCCTATTCAT TCACTTCTCA CATCTGTCAA GGTGCAGTCC 
CAAGCACCCA TGGCTGCTCC TCCCCAATCC ATGAGGCACC TCCAGCCTCT GGTTTACCCT 
GCTCCATCTT CCACTGCTAC CACAGCGCCT CCTCCTGCCT CTTCTCACAT ACACCAACTG 
CAGTCTTCCC CTGCAGTCCC TCAGCAACAG CCAGCTGGTC AGGCACCGCC ACCCCCCCAG 
CAGCAGCAGC ACCCGGCCCT GCAAGGCGCG CTCGTTTCCT CACATCCGCA GCATGTGCAG 
CACCAACAGG CCAAACAACA ACAAGTGATC CAGCATCATC ATCCTTCCCC ACGGCAGCAG 
AAGCAAGAGA CATATGCTGG AGGTCACTTG CGTGATGCTC CTTCCCCTCT CCTCCTGCAT 
TCGCCTCAGG TTCCTCCCTA TCCGGGTCTG GCTCACCCAC CTTCCCCACA GTCTGTGCAA 
CCTAAAAAGC AGGAGATGAG AGGAGCGTCG GTGCTGCAGC CTCAGCCGCT GGTCATGAAA 
GAAGATAAAC GTCATTCGCC TTCACTGCGC CCAGAGGGCT TCTCTCCAGG AATGCGCCCT 
GAACCTCAGA AAGTTCCGGA GGTTCTAAAA GGATCTAGTC ACATCCAGCC ACGACCAGAC 
TTGAAGCCAA TGGATGTTGG ACGTCCCATA CGGCCGACTG ACCAGAGCCT CCCACCTCAA 
GGGATGCCAG AAAAAGAGAA GCAAAAGCAA GAACCTAAGA CACCTGTGGC TCCTAAAAAG 
GACATAAAGA TTAAAAACAT GGGTTCGTGG GCTGGTCTAA TGCAGAAGCC ACCAGTAACC 
CCAACTTCTG CGGGAAAGTC AACAAGTGAC AGCTTTGAGC TGTTCCGGAG GCAGGCCCGC 
GAGAAAGAGG AGCGCGAGAG GGCCCTCAAA CTTCAGGCCG AACAAGCAGA GAGAATGCGC 
AGGGAGCAGG AGAGAATGAG GTCCCGGGAT GAAGATGACG CGCAGGAGCA GGCTAGGAAA 
GCCCACGAGG AGGCACGCCG GCGGCAGGAA CAACAGCAAC AGCAGCAACA ACAACAGCAG 
CAACAGCAAC ACGTGCAGAG CAACCTCCCC GCCGCTCCGT CACCTGCGCA GAGCTCTCAG 
CCAATCATGG ATCAGCGAGA AATGGCTAGA AAGCGGGAAC AGGAGAGGAG ACGAAGGCAG 
GCGATGGCGC CCTCTATAGA TATGAATTTT CAGAGTGAAC TGATGGAGAT ATTTGAACAG 
AACCTGTTCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809168.1
CDS	261..4355
Translation	MSTETGPGTRLRATSGMGDGVEGAQMSGQQLPAAPQPQPQNSSLLNPPPPDIARPSQAKRQTNQLQYLLKTVLKTLWKHQFAWPFQLPVDVVKLNLPDYHKIIKNPMDMGTIKKRLENHYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKISEMPQEETELAVVQSKGRGRGRKEQSEQDTSITPMRTRVLSGSLGDKSAVKPPPVTPVSKPATPTPPTVTRAPTPPQTRPPQQARPPAITQAPISFSPTISQDVVVPTTVAPTPVPQPLPNHPAVIHTAAQPAKTKKGVKRKADTTTPTTHDPLHESSPLPSDSKPPKAGPRKESGRQIRPTKKTEVPDSQLPAPPVLQPQPTPTAEKDSKTSEQLRYCAGIIREMFSKKHQAYAWPFYKPVDVDALGLHDYREIIKHPMDLGTIKVKMDNWDYKDAKEFASDVRLMFSNCYKYNPPDHEVVIMARKLQDVFEMRYAKMPDEPEEAPAPAPSPAPGPPAPSIKGPPPTSSDSSSDSSSDSESSSDSEEERAQRLAELQEQLKAVHEQLAALSQPQPNKPKKKEREKRKEKHKRKEEVEEPRKGRIREPPAKKPKKSVQGSGGTPSIKKEAPPPVARPARPVPPPAPCESSEEETQRCRPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQDKIEAPTSGTGKVKGYSSSESESSSESSTSDSEESDPETAPNQKKKGHSGRESRKHHHPMQQPLAAPPPVMKPPSPTLAPPYPPPSSLDSSHPSLHHPLHSANVFDAVMQLPPDLPPHLTGQTEHCSPPHLNQHALTSPPALHNAMPQQPSRPSNRAAALPPKPVQPPSVSPPLPPPPPQPHHQPSVHIHHHHHHAQPPHALLEDDGPPSPHTGLPSYLQQLQKPQQPPTQSPIHSLLTSVKVQSQAPMAAPPQSMRHLQPLVYPAPSSTATTAPPPASSHIHQLQSSPAVPQQQPAGQAPPPPQQQQHPALQGALVSSHPQHVQHQQAKQQQVIQHHHPSPRQQKQETYAGGHLRDAPSPLLLHSPQVPPYPGLAHPPSPQSVQPKKQEMRGASVLQPQPLVMKEDKRHSPSLRPEGFSPGMRPEPQKVPEVLKGSSHIQPRPDLKPMDVGRPIRPTDQSLPPQGMPEKEKQKQEPKTPVAPKKDIKIKNMGSWAGLMQKPPVTPTSAGKSTSDSFELFRRQAREKEERERALKLQAEQAERMRREQERMRSRDEDDAQEQARKAHEEARRRQEQQQQQQQQQQQQQHVQSNLPAAPSPAQSSQPIMDQREMARKREQERRRRQAMAPSIDMNFQSELMEIFEQNLFS

Target ORF information:

RefSeq Version	XM_012953714.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953714.2

ORF Insert Sequence:

ATGTCAACCG AGACTGGGCC TGGTACACGT CTGAGAGCCA CATCTGGAAT GGGTGACGGA 
GTAGAAGGGG CTCAGATGTC GGGCCAACAG CTGCCTGCCG CTCCCCAGCC CCAGCCACAG 
AACTCATCGC TGCTCAATCC TCCTCCTCCT GATATCGCAA GACCCAGCCA AGCAAAGCGC 
CAGACCAACC AGCTGCAGTA CTTACTTAAG ACAGTGCTCA AGACATTGTG GAAGCACCAG 
TTTGCATGGC CTTTCCAGCT ACCTGTTGAT GTGGTCAAGC TCAATCTGCC TGATTATCAT 
AAAATAATTA AAAACCCAAT GGACATGGGC ACCATTAAAA AGCGCCTCGA GAACCATTAT 
TACTGGAACG CCCAAGAATG TATCCAGGAC TTCAACACAA TGTTTACAAA CTGCTACATC 
TATAACAAGC CTGGAGATGA CATAGTGCTG ATGGCCGAAG CCCTTGAGAA GCTTTTTCTG 
CAGAAGATCT CAGAGATGCC CCAGGAAGAA ACCGAACTGG CAGTTGTGCA GAGCAAAGGC 
CGTGGCCGGG GAAGAAAGGA GCAAAGTGAG CAAGATACTT CAATCACCCC CATGAGAACC 
CGAGTCCTGT CTGGGTCACT AGGAGACAAG AGCGCCGTCA AACCTCCCCC TGTTACACCT 
GTGTCCAAGC CTGCAACCCC AACGCCCCCT ACTGTGACCA GGGCTCCCAC CCCTCCACAA 
ACCAGACCAC CCCAACAAGC TCGCCCTCCA GCTATCACTC AAGCCCCCAT CAGTTTCTCT 
CCCACCATCA GTCAGGATGT GGTGGTTCCA ACGACCGTAG CCCCAACACC AGTTCCTCAG 
CCACTTCCAA ATCATCCTGC AGTCATCCAC ACTGCTGCCC AGCCTGCAAA GACAAAGAAA 
GGTGTGAAGA GGAAAGCAGA CACCACAACC CCCACTACCC ATGACCCGCT CCATGAGTCC 
TCCCCACTCC CTAGTGACTC CAAGCCTCCA AAGGCTGGCC CTCGAAAGGA GAGTGGGCGT 
CAGATTCGAC CAACTAAGAA AACCGAGGTG CCTGACTCTC AACTTCCTGC CCCCCCTGTT 
TTGCAACCCC AACCTACACC CACTGCTGAG AAGGACAGCA AGACAAGCGA GCAGTTACGG 
TATTGTGCAG GCATTATCCG GGAAATGTTT TCCAAAAAGC ATCAGGCCTA TGCGTGGCCA 
TTTTACAAGC CCGTTGATGT GGATGCCCTT GGGTTACATG ACTACCGTGA AATTATCAAG 
CATCCAATGG ACCTAGGCAC CATTAAGGTA AAGATGGATA ATTGGGACTA CAAGGACGCA 
AAGGAATTTG CATCTGATGT GCGGCTTATG TTCTCAAACT GTTACAAGTA CAATCCCCCT 
GATCATGAAG TTGTGATAAT GGCTCGCAAG TTGCAGGATG TTTTTGAAAT GCGCTATGCC 
AAAATGCCTG ATGAACCTGA GGAGGCGCCA GCTCCTGCAC CCTCTCCTGC TCCAGGGCCC 
CCAGCCCCAT CCATCAAAGG CCCTCCGCCA ACCTCTAGTG ACAGCAGCAG CGATAGCTCA 
TCTGATAGTG AAAGCAGTTC AGACAGTGAG GAGGAACGGG CACAAAGACT GGCAGAGCTT 
CAGGAACAGT TGAAAGCTGT ACATGAGCAG TTGGCAGCTC TATCTCAGCC ACAGCCGAAC 
AAACCAAAGA AGAAAGAGCG TGAAAAGCGG AAGGAAAAGC ACAAACGAAA AGAAGAGGTG 
GAGGAGCCTA GAAAGGGCCG TATCCGAGAG CCACCAGCCA AGAAACCCAA GAAAAGTGTT 
CAAGGGTCCG GAGGTACACC AAGCATCAAG AAAGAAGCAC CTCCCCCTGT GGCACGCCCT 
GCTCGTCCAG TGCCACCTCC GGCACCATGT GAGTCCTCTG AGGAAGAAAC GCAACGATGT 
CGGCCAATGT CATACGAAGA GAAACGCCAG CTAAGCTTGG ACATTAATAA GCTGCCAGGC 
GAAAAACTGG GCCGCGTCGT GCACATCATC CAGTCTCGCG AGCCTTCCCT GAAGAACTCC 
AATCCAGATG AGATTGAGAT AGACTTTGAG ACACTTAAAC CGTCCACACT GCGAGAGTTG 
GAGAGATATG TTACCTCCTG TTTGAGAAAG AAGCGAAAAC CACAGGATAA AATAGAAGCT 
CCCACATCTG GCACTGGTAA AGTAAAAGGC TATTCCTCTT CAGAGTCTGA GAGCTCAAGT 
GAGTCCAGCA CGTCAGACAG CGAGGAGTCC GACCCAGAAA CAGCCCCAAA TCAGAAGAAA 
AAGGGGCACT CGGGGAGAGA GTCTAGAAAG CATCATCACC CTATGCAACA GCCTCTTGCC 
GCACCACCCC CTGTCATGAA GCCTCCGTCT CCCACCTTAG CACCACCTTA CCCTCCGCCT 
TCCAGCCTGG ACTCCTCACA TCCTTCTCTC CACCACCCTC TCCACTCTGC GAATGTTTTT 
GATGCAGTCA TGCAGCTTCC TCCTGATCTG CCTCCTCACC TTACTGGTCA GACAGAGCAC 
TGCTCGCCCC CACACCTCAA CCAACATGCC CTGACAAGCC CTCCAGCCCT ACACAACGCA 
ATGCCCCAGC AGCCCTCCAG GCCATCCAAC CGTGCTGCTG CCCTTCCTCC AAAACCTGTT 
CAGCCTCCCT CTGTCTCTCC TCCTCTTCCT CCTCCTCCTC CGCAGCCCCA CCACCAGCCT 
TCTGTGCATA TCCATCATCA CCACCACCAT GCACAGCCCC CTCATGCTCT TCTGGAAGAT 
GATGGGCCTC CCTCTCCTCA CACAGGTCTC CCTTCCTACC TGCAACAGCT TCAGAAGCCA 
CAGCAACCTC CTACTCAGTC TCCTATTCAT TCACTTCTCA CATCTGTCAA GGTGCAGTCC 
CAAGCACCCA TGGCTGCTCC TCCCCAATCC ATGAGGCACC TCCAGCCTCT GGTTTACCCT 
GCTCCATCTT CCACTGCTAC CACAGCGCCT CCTCCTGCCT CTTCTCACAT ACACCAACTG 
CAGTCTTCCC CTGCAGTCCC TCAGCAACAG CCAGCTGGTC AGGCACCGCC ACCCCCCCAG 
CAGCAGCAGC ACCCGGCCCT GCAAGGCGCG CTCGTTTCCT CACATCCGCA GCATGTGCAG 
CACCAACAGG CCAAACAACA ACAAGTGATC CAGCATCATC ATCCTTCCCC ACGGCAGCAG 
AAGCAAGAGA CATATGCTGG AGGTCACTTG CGTGATGCTC CTTCCCCTCT CCTCCTGCAT 
TCGCCTCAGG TTCCTCCCTA TCCGGGTCTG GCTCACCCAC CTTCCCCACA GTCTGTGCAA 
CCTAAAAAGC AGGAGATGAG AGGAGCGTCG GTGCTGCAGC CTCAGCCGCT GGTCATGAAA 
GAAGATAAAC GTCATTCGCC TTCACTGCGC CCAGAGGGCT TCTCTCCAGG AATGCGCCCT 
GAACCTCAGA AAGTTCCGGA GGTTCTAAAA GGATCTAGTC ACATCCAGCC ACGACCAGAC 
TTGAAGCCAA TGGATGTTGG ACGTCCCATA CGGCCGACTG ACCAGAGCCT CCCACCTCAA 
GGGATGCCAG AAAAAGAGAA GCAAAAGCAA GAACCTAAGA CACCTGTGGC TCCTAAAAAG 
GACATAAAGA TTAAAAACAT GGGTTCGTGG GCTGGTCTAA TGCAGAAGCC ACCAGTAACC 
CCAACTTCTG CGGGAAAGTC AACAAGTGAC AGCTTTGAGC TGTTCCGGAG GCAGGCCCGC 
GAGAAAGAGG AGCGCGAGAG GGCCCTCAAA CTTCAGGCCG AACAAGCAGA GAGAATGCGC 
AGGGAGCAGG AGAGAATGAG GTCCCGGGAT GAAGATGACG CGCAGGAGCA GGCTAGGAAA 
GCCCACGAGG AGGCACGCCG GCGGCAGGAA CAACAGCAAC AGCAGCAACA ACAACAGCAG 
CAACAGCAAC ACGTGCAGAG CAACCTCCCC GCCGCTCCGT CACCTGCGCA GAGCTCTCAG 
CCAATCATGG ATCAGCGAGA AATGGCTAGA AAGCGGGAAC AGGAGAGGAG ACGAAGGCAG 
GCGATGGCGC CCTCTATAGA TATGAATTTT CAGAGTGAAC TGATGGAGAT ATTTGAACAG 
AACCTGTTCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brd4 ( XM_012953713.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809167.1 Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X2, mRNA.

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CloneID	OXa32766
Clone ID Related Accession (Same CDS sequence)	XM_012953714.2 , XM_012953713.2 , XM_012953714.1 , XM_012953713.3
Accession Version	XM_012953713.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012953713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAACCG AGACTGGGCC TGGTACACGT CTGAGAGCCA CATCTGGAAT GGGTGACGGA 
GTAGAAGGGG CTCAGATGTC GGGCCAACAG CTGCCTGCCG CTCCCCAGCC CCAGCCACAG 
AACTCATCGC TGCTCAATCC TCCTCCTCCT GATATCGCAA GACCCAGCCA AGCAAAGCGC 
CAGACCAACC AGCTGCAGTA CTTACTTAAG ACAGTGCTCA AGACATTGTG GAAGCACCAG 
TTTGCATGGC CTTTCCAGCT ACCTGTTGAT GTGGTCAAGC TCAATCTGCC TGATTATCAT 
AAAATAATTA AAAACCCAAT GGACATGGGC ACCATTAAAA AGCGCCTCGA GAACCATTAT 
TACTGGAACG CCCAAGAATG TATCCAGGAC TTCAACACAA TGTTTACAAA CTGCTACATC 
TATAACAAGC CTGGAGATGA CATAGTGCTG ATGGCCGAAG CCCTTGAGAA GCTTTTTCTG 
CAGAAGATCT CAGAGATGCC CCAGGAAGAA ACCGAACTGG CAGTTGTGCA GAGCAAAGGC 
CGTGGCCGGG GAAGAAAGGA GCAAAGTGAG CAAGATACTT CAATCACCCC CATGAGAACC 
CGAGTCCTGT CTGGGTCACT AGGAGACAAG AGCGCCGTCA AACCTCCCCC TGTTACACCT 
GTGTCCAAGC CTGCAACCCC AACGCCCCCT ACTGTGACCA GGGCTCCCAC CCCTCCACAA 
ACCAGACCAC CCCAACAAGC TCGCCCTCCA GCTATCACTC AAGCCCCCAT CAGTTTCTCT 
CCCACCATCA GTCAGGATGT GGTGGTTCCA ACGACCGTAG CCCCAACACC AGTTCCTCAG 
CCACTTCCAA ATCATCCTGC AGTCATCCAC ACTGCTGCCC AGCCTGCAAA GACAAAGAAA 
GGTGTGAAGA GGAAAGCAGA CACCACAACC CCCACTACCC ATGACCCGCT CCATGAGTCC 
TCCCCACTCC CTAGTGACTC CAAGCCTCCA AAGGCTGGCC CTCGAAAGGA GAGTGGGCGT 
CAGATTCGAC CAACTAAGAA AACCGAGGTG CCTGACTCTC AACTTCCTGC CCCCCCTGTT 
TTGCAACCCC AACCTACACC CACTGCTGAG AAGGACAGCA AGACAAGCGA GCAGTTACGG 
TATTGTGCAG GCATTATCCG GGAAATGTTT TCCAAAAAGC ATCAGGCCTA TGCGTGGCCA 
TTTTACAAGC CCGTTGATGT GGATGCCCTT GGGTTACATG ACTACCGTGA AATTATCAAG 
CATCCAATGG ACCTAGGCAC CATTAAGGTA AAGATGGATA ATTGGGACTA CAAGGACGCA 
AAGGAATTTG CATCTGATGT GCGGCTTATG TTCTCAAACT GTTACAAGTA CAATCCCCCT 
GATCATGAAG TTGTGATAAT GGCTCGCAAG TTGCAGGATG TTTTTGAAAT GCGCTATGCC 
AAAATGCCTG ATGAACCTGA GGAGGCGCCA GCTCCTGCAC CCTCTCCTGC TCCAGGGCCC 
CCAGCCCCAT CCATCAAAGG CCCTCCGCCA ACCTCTAGTG ACAGCAGCAG CGATAGCTCA 
TCTGATAGTG AAAGCAGTTC AGACAGTGAG GAGGAACGGG CACAAAGACT GGCAGAGCTT 
CAGGAACAGT TGAAAGCTGT ACATGAGCAG TTGGCAGCTC TATCTCAGCC ACAGCCGAAC 
AAACCAAAGA AGAAAGAGCG TGAAAAGCGG AAGGAAAAGC ACAAACGAAA AGAAGAGGTG 
GAGGAGCCTA GAAAGGGCCG TATCCGAGAG CCACCAGCCA AGAAACCCAA GAAAAGTGTT 
CAAGGGTCCG GAGGTACACC AAGCATCAAG AAAGAAGCAC CTCCCCCTGT GGCACGCCCT 
GCTCGTCCAG TGCCACCTCC GGCACCATGT GAGTCCTCTG AGGAAGAAAC GCAACGATGT 
CGGCCAATGT CATACGAAGA GAAACGCCAG CTAAGCTTGG ACATTAATAA GCTGCCAGGC 
GAAAAACTGG GCCGCGTCGT GCACATCATC CAGTCTCGCG AGCCTTCCCT GAAGAACTCC 
AATCCAGATG AGATTGAGAT AGACTTTGAG ACACTTAAAC CGTCCACACT GCGAGAGTTG 
GAGAGATATG TTACCTCCTG TTTGAGAAAG AAGCGAAAAC CACAGGATAA AATAGAAGCT 
CCCACATCTG GCACTGGTAA AGTAAAAGGC TATTCCTCTT CAGAGTCTGA GAGCTCAAGT 
GAGTCCAGCA CGTCAGACAG CGAGGAGTCC GACCCAGAAA CAGCCCCAAA TCAGAAGAAA 
AAGGGGCACT CGGGGAGAGA GTCTAGAAAG CATCATCACC CTATGCAACA GCCTCTTGCC 
GCACCACCCC CTGTCATGAA GCCTCCGTCT CCCACCTTAG CACCACCTTA CCCTCCGCCT 
TCCAGCCTGG ACTCCTCACA TCCTTCTCTC CACCACCCTC TCCACTCTGC GAATGTTTTT 
GATGCAGTCA TGCAGCTTCC TCCTGATCTG CCTCCTCACC TTACTGGTCA GACAGAGCAC 
TGCTCGCCCC CACACCTCAA CCAACATGCC CTGACAAGCC CTCCAGCCCT ACACAACGCA 
ATGCCCCAGC AGCCCTCCAG GCCATCCAAC CGTGCTGCTG CCCTTCCTCC AAAACCTGTT 
CAGCCTCCCT CTGTCTCTCC TCCTCTTCCT CCTCCTCCTC CGCAGCCCCA CCACCAGCCT 
TCTGTGCATA TCCATCATCA CCACCACCAT GCACAGCCCC CTCATGCTCT TCTGGAAGAT 
GATGGGCCTC CCTCTCCTCA CACAGGTCTC CCTTCCTACC TGCAACAGCT TCAGAAGCCA 
CAGCAACCTC CTACTCAGTC TCCTATTCAT TCACTTCTCA CATCTGTCAA GGTGCAGTCC 
CAAGCACCCA TGGCTGCTCC TCCCCAATCC ATGAGGCACC TCCAGCCTCT GGTTTACCCT 
GCTCCATCTT CCACTGCTAC CACAGCGCCT CCTCCTGCCT CTTCTCACAT ACACCAACTG 
CAGTCTTCCC CTGCAGTCCC TCAGCAACAG CCAGCTGGTC AGGCACCGCC ACCCCCCCAG 
CAGCAGCAGC ACCCGGCCCT GCAAGGCGCG CTCGTTTCCT CACATCCGCA GCATGTGCAG 
CACCAACAGG CCAAACAACA ACAAGTGATC CAGCATCATC ATCCTTCCCC ACGGCAGCAG 
AAGCAAGAGA CATATGCTGG AGGTCACTTG CGTGATGCTC CTTCCCCTCT CCTCCTGCAT 
TCGCCTCAGG TTCCTCCCTA TCCGGGTCTG GCTCACCCAC CTTCCCCACA GTCTGTGCAA 
CCTAAAAAGC AGGAGATGAG AGGAGCGTCG GTGCTGCAGC CTCAGCCGCT GGTCATGAAA 
GAAGATAAAC GTCATTCGCC TTCACTGCGC CCAGAGGGCT TCTCTCCAGG AATGCGCCCT 
GAACCTCAGA AAGTTCCGGA GGTTCTAAAA GGATCTAGTC ACATCCAGCC ACGACCAGAC 
TTGAAGCCAA TGGATGTTGG ACGTCCCATA CGGCCGACTG ACCAGAGCCT CCCACCTCAA 
GGGATGCCAG AAAAAGAGAA GCAAAAGCAA GAACCTAAGA CACCTGTGGC TCCTAAAAAG 
GACATAAAGA TTAAAAACAT GGGTTCGTGG GCTGGTCTAA TGCAGAAGCC ACCAGTAACC 
CCAACTTCTG CGGGAAAGTC AACAAGTGAC AGCTTTGAGC TGTTCCGGAG GCAGGCCCGC 
GAGAAAGAGG AGCGCGAGAG GGCCCTCAAA CTTCAGGCCG AACAAGCAGA GAGAATGCGC 
AGGGAGCAGG AGAGAATGAG GTCCCGGGAT GAAGATGACG CGCAGGAGCA GGCTAGGAAA 
GCCCACGAGG AGGCACGCCG GCGGCAGGAA CAACAGCAAC AGCAGCAACA ACAACAGCAG 
CAACAGCAAC ACGTGCAGAG CAACCTCCCC GCCGCTCCGT CACCTGCGCA GAGCTCTCAG 
CCAATCATGG ATCAGCGAGA AATGGCTAGA AAGCGGGAAC AGGAGAGGAG ACGAAGGCAG 
GCGATGGCGC CCTCTATAGA TATGAATTTT CAGAGTGAAC TGATGGAGAT ATTTGAACAG 
AACCTGTTCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809167.1
CDS	246..4340
Translation	MSTETGPGTRLRATSGMGDGVEGAQMSGQQLPAAPQPQPQNSSLLNPPPPDIARPSQAKRQTNQLQYLLKTVLKTLWKHQFAWPFQLPVDVVKLNLPDYHKIIKNPMDMGTIKKRLENHYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKISEMPQEETELAVVQSKGRGRGRKEQSEQDTSITPMRTRVLSGSLGDKSAVKPPPVTPVSKPATPTPPTVTRAPTPPQTRPPQQARPPAITQAPISFSPTISQDVVVPTTVAPTPVPQPLPNHPAVIHTAAQPAKTKKGVKRKADTTTPTTHDPLHESSPLPSDSKPPKAGPRKESGRQIRPTKKTEVPDSQLPAPPVLQPQPTPTAEKDSKTSEQLRYCAGIIREMFSKKHQAYAWPFYKPVDVDALGLHDYREIIKHPMDLGTIKVKMDNWDYKDAKEFASDVRLMFSNCYKYNPPDHEVVIMARKLQDVFEMRYAKMPDEPEEAPAPAPSPAPGPPAPSIKGPPPTSSDSSSDSSSDSESSSDSEEERAQRLAELQEQLKAVHEQLAALSQPQPNKPKKKEREKRKEKHKRKEEVEEPRKGRIREPPAKKPKKSVQGSGGTPSIKKEAPPPVARPARPVPPPAPCESSEEETQRCRPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQDKIEAPTSGTGKVKGYSSSESESSSESSTSDSEESDPETAPNQKKKGHSGRESRKHHHPMQQPLAAPPPVMKPPSPTLAPPYPPPSSLDSSHPSLHHPLHSANVFDAVMQLPPDLPPHLTGQTEHCSPPHLNQHALTSPPALHNAMPQQPSRPSNRAAALPPKPVQPPSVSPPLPPPPPQPHHQPSVHIHHHHHHAQPPHALLEDDGPPSPHTGLPSYLQQLQKPQQPPTQSPIHSLLTSVKVQSQAPMAAPPQSMRHLQPLVYPAPSSTATTAPPPASSHIHQLQSSPAVPQQQPAGQAPPPPQQQQHPALQGALVSSHPQHVQHQQAKQQQVIQHHHPSPRQQKQETYAGGHLRDAPSPLLLHSPQVPPYPGLAHPPSPQSVQPKKQEMRGASVLQPQPLVMKEDKRHSPSLRPEGFSPGMRPEPQKVPEVLKGSSHIQPRPDLKPMDVGRPIRPTDQSLPPQGMPEKEKQKQEPKTPVAPKKDIKIKNMGSWAGLMQKPPVTPTSAGKSTSDSFELFRRQAREKEERERALKLQAEQAERMRREQERMRSRDEDDAQEQARKAHEEARRRQEQQQQQQQQQQQQQHVQSNLPAAPSPAQSSQPIMDQREMARKREQERRRRQAMAPSIDMNFQSELMEIFEQNLFS

Target ORF information:

RefSeq Version	XM_012953713.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953713.3

ORF Insert Sequence:

ATGTCAACCG AGACTGGGCC TGGTACACGT CTGAGAGCCA CATCTGGAAT GGGTGACGGA 
GTAGAAGGGG CTCAGATGTC GGGCCAACAG CTGCCTGCCG CTCCCCAGCC CCAGCCACAG 
AACTCATCGC TGCTCAATCC TCCTCCTCCT GATATCGCAA GACCCAGCCA AGCAAAGCGC 
CAGACCAACC AGCTGCAGTA CTTACTTAAG ACAGTGCTCA AGACATTGTG GAAGCACCAG 
TTTGCATGGC CTTTCCAGCT ACCTGTTGAT GTGGTCAAGC TCAATCTGCC TGATTATCAT 
AAAATAATTA AAAACCCAAT GGACATGGGC ACCATTAAAA AGCGCCTCGA GAACCATTAT 
TACTGGAACG CCCAAGAATG TATCCAGGAC TTCAACACAA TGTTTACAAA CTGCTACATC 
TATAACAAGC CTGGAGATGA CATAGTGCTG ATGGCCGAAG CCCTTGAGAA GCTTTTTCTG 
CAGAAGATCT CAGAGATGCC CCAGGAAGAA ACCGAACTGG CAGTTGTGCA GAGCAAAGGC 
CGTGGCCGGG GAAGAAAGGA GCAAAGTGAG CAAGATACTT CAATCACCCC CATGAGAACC 
CGAGTCCTGT CTGGGTCACT AGGAGACAAG AGCGCCGTCA AACCTCCCCC TGTTACACCT 
GTGTCCAAGC CTGCAACCCC AACGCCCCCT ACTGTGACCA GGGCTCCCAC CCCTCCACAA 
ACCAGACCAC CCCAACAAGC TCGCCCTCCA GCTATCACTC AAGCCCCCAT CAGTTTCTCT 
CCCACCATCA GTCAGGATGT GGTGGTTCCA ACGACCGTAG CCCCAACACC AGTTCCTCAG 
CCACTTCCAA ATCATCCTGC AGTCATCCAC ACTGCTGCCC AGCCTGCAAA GACAAAGAAA 
GGTGTGAAGA GGAAAGCAGA CACCACAACC CCCACTACCC ATGACCCGCT CCATGAGTCC 
TCCCCACTCC CTAGTGACTC CAAGCCTCCA AAGGCTGGCC CTCGAAAGGA GAGTGGGCGT 
CAGATTCGAC CAACTAAGAA AACCGAGGTG CCTGACTCTC AACTTCCTGC CCCCCCTGTT 
TTGCAACCCC AACCTACACC CACTGCTGAG AAGGACAGCA AGACAAGCGA GCAGTTACGG 
TATTGTGCAG GCATTATCCG GGAAATGTTT TCCAAAAAGC ATCAGGCCTA TGCGTGGCCA 
TTTTACAAGC CCGTTGATGT GGATGCCCTT GGGTTACATG ACTACCGTGA AATTATCAAG 
CATCCAATGG ACCTAGGCAC CATTAAGGTA AAGATGGATA ATTGGGACTA CAAGGACGCA 
AAGGAATTTG CATCTGATGT GCGGCTTATG TTCTCAAACT GTTACAAGTA CAATCCCCCT 
GATCATGAAG TTGTGATAAT GGCTCGCAAG TTGCAGGATG TTTTTGAAAT GCGCTATGCC 
AAAATGCCTG ATGAACCTGA GGAGGCGCCA GCTCCTGCAC CCTCTCCTGC TCCAGGGCCC 
CCAGCCCCAT CCATCAAAGG CCCTCCGCCA ACCTCTAGTG ACAGCAGCAG CGATAGCTCA 
TCTGATAGTG AAAGCAGTTC AGACAGTGAG GAGGAACGGG CACAAAGACT GGCAGAGCTT 
CAGGAACAGT TGAAAGCTGT ACATGAGCAG TTGGCAGCTC TATCTCAGCC ACAGCCGAAC 
AAACCAAAGA AGAAAGAGCG TGAAAAGCGG AAGGAAAAGC ACAAACGAAA AGAAGAGGTG 
GAGGAGCCTA GAAAGGGCCG TATCCGAGAG CCACCAGCCA AGAAACCCAA GAAAAGTGTT 
CAAGGGTCCG GAGGTACACC AAGCATCAAG AAAGAAGCAC CTCCCCCTGT GGCACGCCCT 
GCTCGTCCAG TGCCACCTCC GGCACCATGT GAGTCCTCTG AGGAAGAAAC GCAACGATGT 
CGGCCAATGT CATACGAAGA GAAACGCCAG CTAAGCTTGG ACATTAATAA GCTGCCAGGC 
GAAAAACTGG GCCGCGTCGT GCACATCATC CAGTCTCGCG AGCCTTCCCT GAAGAACTCC 
AATCCAGATG AGATTGAGAT AGACTTTGAG ACACTTAAAC CGTCCACACT GCGAGAGTTG 
GAGAGATATG TTACCTCCTG TTTGAGAAAG AAGCGAAAAC CACAGGATAA AATAGAAGCT 
CCCACATCTG GCACTGGTAA AGTAAAAGGC TATTCCTCTT CAGAGTCTGA GAGCTCAAGT 
GAGTCCAGCA CGTCAGACAG CGAGGAGTCC GACCCAGAAA CAGCCCCAAA TCAGAAGAAA 
AAGGGGCACT CGGGGAGAGA GTCTAGAAAG CATCATCACC CTATGCAACA GCCTCTTGCC 
GCACCACCCC CTGTCATGAA GCCTCCGTCT CCCACCTTAG CACCACCTTA CCCTCCGCCT 
TCCAGCCTGG ACTCCTCACA TCCTTCTCTC CACCACCCTC TCCACTCTGC GAATGTTTTT 
GATGCAGTCA TGCAGCTTCC TCCTGATCTG CCTCCTCACC TTACTGGTCA GACAGAGCAC 
TGCTCGCCCC CACACCTCAA CCAACATGCC CTGACAAGCC CTCCAGCCCT ACACAACGCA 
ATGCCCCAGC AGCCCTCCAG GCCATCCAAC CGTGCTGCTG CCCTTCCTCC AAAACCTGTT 
CAGCCTCCCT CTGTCTCTCC TCCTCTTCCT CCTCCTCCTC CGCAGCCCCA CCACCAGCCT 
TCTGTGCATA TCCATCATCA CCACCACCAT GCACAGCCCC CTCATGCTCT TCTGGAAGAT 
GATGGGCCTC CCTCTCCTCA CACAGGTCTC CCTTCCTACC TGCAACAGCT TCAGAAGCCA 
CAGCAACCTC CTACTCAGTC TCCTATTCAT TCACTTCTCA CATCTGTCAA GGTGCAGTCC 
CAAGCACCCA TGGCTGCTCC TCCCCAATCC ATGAGGCACC TCCAGCCTCT GGTTTACCCT 
GCTCCATCTT CCACTGCTAC CACAGCGCCT CCTCCTGCCT CTTCTCACAT ACACCAACTG 
CAGTCTTCCC CTGCAGTCCC TCAGCAACAG CCAGCTGGTC AGGCACCGCC ACCCCCCCAG 
CAGCAGCAGC ACCCGGCCCT GCAAGGCGCG CTCGTTTCCT CACATCCGCA GCATGTGCAG 
CACCAACAGG CCAAACAACA ACAAGTGATC CAGCATCATC ATCCTTCCCC ACGGCAGCAG 
AAGCAAGAGA CATATGCTGG AGGTCACTTG CGTGATGCTC CTTCCCCTCT CCTCCTGCAT 
TCGCCTCAGG TTCCTCCCTA TCCGGGTCTG GCTCACCCAC CTTCCCCACA GTCTGTGCAA 
CCTAAAAAGC AGGAGATGAG AGGAGCGTCG GTGCTGCAGC CTCAGCCGCT GGTCATGAAA 
GAAGATAAAC GTCATTCGCC TTCACTGCGC CCAGAGGGCT TCTCTCCAGG AATGCGCCCT 
GAACCTCAGA AAGTTCCGGA GGTTCTAAAA GGATCTAGTC ACATCCAGCC ACGACCAGAC 
TTGAAGCCAA TGGATGTTGG ACGTCCCATA CGGCCGACTG ACCAGAGCCT CCCACCTCAA 
GGGATGCCAG AAAAAGAGAA GCAAAAGCAA GAACCTAAGA CACCTGTGGC TCCTAAAAAG 
GACATAAAGA TTAAAAACAT GGGTTCGTGG GCTGGTCTAA TGCAGAAGCC ACCAGTAACC 
CCAACTTCTG CGGGAAAGTC AACAAGTGAC AGCTTTGAGC TGTTCCGGAG GCAGGCCCGC 
GAGAAAGAGG AGCGCGAGAG GGCCCTCAAA CTTCAGGCCG AACAAGCAGA GAGAATGCGC 
AGGGAGCAGG AGAGAATGAG GTCCCGGGAT GAAGATGACG CGCAGGAGCA GGCTAGGAAA 
GCCCACGAGG AGGCACGCCG GCGGCAGGAA CAACAGCAAC AGCAGCAACA ACAACAGCAG 
CAACAGCAAC ACGTGCAGAG CAACCTCCCC GCCGCTCCGT CACCTGCGCA GAGCTCTCAG 
CCAATCATGG ATCAGCGAGA AATGGCTAGA AAGCGGGAAC AGGAGAGGAG ACGAAGGCAG 
GCGATGGCGC CCTCTATAGA TATGAATTTT CAGAGTGAAC TGATGGAGAT ATTTGAACAG 
AACCTGTTCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brd4 ( XM_018091980.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947469.1 Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa43062
Clone ID Related Accession (Same CDS sequence)	XM_018091980.2 , XM_018091980.1
Accession Version	XM_018091980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCATGA CGTCACGGTA CGTAGCGGCG GCGCTGGCGC TGTGCGAGGA AGGAGCCCAG 
GGAGCGAACG CTCGGTCACC TGGCTCTGCG TGCGATGGGA GCTACGCTAT GTCAACCGAG 
ACTGGGCCTG GTACACGTCT GAGAGCCACA TCTGGAATGG GTGACGGAGT AGAAGGGGCT 
CAGATGTCGG GCCAACAGCT GCCTGCCGCT CCCCAGCCCC AGCCACAGAA CTCATCGCTG 
CTCAATCCTC CTCCTCCTGA TATCGCAAGA CCCAGCCAAG CAAAGCGCCA GACCAACCAG 
CTGCAGTACT TACTTAAGAC AGTGCTCAAG ACATTGTGGA AGCACCAGTT TGCATGGCCT 
TTCCAGCTAC CTGTTGATGT GGTCAAGCTC AATCTGCCTG ATTATCATAA AATAATTAAA 
AACCCAATGG ACATGGGCAC CATTAAAAAG CGCCTCGAGA ACCATTATTA CTGGAACGCC 
CAAGAATGTA TCCAGGACTT CAACACAATG TTTACAAACT GCTACATCTA TAACAAGCCT 
GGAGATGACA TAGTGCTGAT GGCCGAAGCC CTTGAGAAGC TTTTTCTGCA GAAGATCTCA 
GAGATGCCCC AGGAAGAAAC CGAACTGGCA GTTGTGCAGA GCAAAGGCCG TGGCCGGGGA 
AGAAAGGAGC AAAGTGAGCA AGATACTTCA ATCACCCCCA TGAGAACCCG AGTCCTGTCT 
GGGTCACTAG GAGACAAGAG CGCCGTCAAA CCTCCCCCTG TTACACCTGT GTCCAAGCCT 
GCAACCCCAA CGCCCCCTAC TGTGACCAGG GCTCCCACCC CTCCACAAAC CAGACCACCC 
CAACAAGCTC GCCCTCCAGC TATCACTCAA GCCCCCATCA GTTTCTCTCC CACCATCAGT 
CAGGATGTGG TGGTTCCAAC GACCGTAGCC CCAACACCAG TTCCTCAGCC ACTTCCAAAT 
CATCCTGCAG TCATCCACAC TGCTGCCCAG CCTGCAAAGA CAAAGAAAGG TGTGAAGAGG 
AAAGCAGACA CCACAACCCC CACTACCCAT GACCCGCTCC ATGAGTCCTC CCCACTCCCT 
AGTGACTCCA AGCCTCCAAA GGCTGGCCCT CGAAAGGAGA GTGGGCGTCA GATTCGACCA 
ACTAAGAAAA CCGAGGTGCC TGACTCTCAA CTTCCTGCCC CCCCTGTTTT GCAACCCCAA 
CCTACACCCA CTGCTGAGAA GGACAGCAAG ACAAGCGAGC AGTTACGGTA TTGTGCAGGC 
ATTATCCGGG AAATGTTTTC CAAAAAGCAT CAGGCCTATG CGTGGCCATT TTACAAGCCC 
GTTGATGTGG ATGCCCTTGG GTTACATGAC TACCGTGAAA TTATCAAGCA TCCAATGGAC 
CTAGGCACCA TTAAGGTAAA GATGGATAAT TGGGACTACA AGGACGCAAA GGAATTTGCA 
TCTGATGTGC GGCTTATGTT CTCAAACTGT TACAAGTACA ATCCCCCTGA TCATGAAGTT 
GTGATAATGG CTCGCAAGTT GCAGGATGTT TTTGAAATGC GCTATGCCAA AATGCCTGAT 
GAACCTGAGG AGGCGCCAGC TCCTGCACCC TCTCCTGCTC CAGGGCCCCC AGCCCCATCC 
ATCAAAGGCC CTCCGCCAAC CTCTAGTGAC AGCAGCAGCG ATAGCTCATC TGATAGTGAA 
AGCAGTTCAG ACAGTGAGGA GGAACGGGCA CAAAGACTGG CAGAGCTTCA GGAACAGTTG 
AAAGCTGTAC ATGAGCAGTT GGCAGCTCTA TCTCAGCCAC AGCCGAACAA ACCAAAGAAG 
AAAGAGCGTG AAAAGCGGAA GGAAAAGCAC AAACGAAAAG AAGAGGTGGA GGAGCCTAGA 
AAGGGCCGTA TCCGAGAGCC ACCAGCCAAG AAACCCAAGA AAAGTGTTCA AGGGTCCGGA 
GGTACACCAA GCATCAAGAA AGAAGCACCT CCCCCTGTGG CACGCCCTGC TCGTCCAGTG 
CCACCTCCGG CACCATGTGA GTCCTCTGAG GAAGAAACGC AACGATGTCG GCCAATGTCA 
TACGAAGAGA AACGCCAGCT AAGCTTGGAC ATTAATAAGC TGCCAGGCGA AAAACTGGGC 
CGCGTCGTGC ACATCATCCA GTCTCGCGAG CCTTCCCTGA AGAACTCCAA TCCAGATGAG 
ATTGAGATAG ACTTTGAGAC ACTTAAACCG TCCACACTGC GAGAGTTGGA GAGATATGTT 
ACCTCCTGTT TGAGAAAGAA GCGAAAACCA CAGGATAAAA TAGAAGCTCC CACATCTGGC 
ACTGGTAAAG TAAAAGGCTA TTCCTCTTCA GAGTCTGAGA GCTCAAGTGA GTCCAGCACG 
TCAGACAGCG AGGAGTCCGA CCCAGAAACA GCCCCAAATC AGAAGAAAAA GGGGCACTCG 
GGGAGAGAGT CTAGAAAGCA TCATCACCCT ATGCAACAGC CTCTTGCCGC ACCACCCCCT 
GTCATGAAGC CTCCGTCTCC CACCTTAGCA CCACCTTACC CTCCGCCTTC CAGCCTGGAC 
TCCTCACATC CTTCTCTCCA CCACCCTCTC CACTCTGCGA ATGTTTTTGA TGCAGTCATG 
CAGCTTCCTC CTGATCTGCC TCCTCACCTT ACTGGTCAGA CAGAGCACTG CTCGCCCCCA 
CACCTCAACC AACATGCCCT GACAAGCCCT CCAGCCCTAC ACAACGCAAT GCCCCAGCAG 
CCCTCCAGGC CATCCAACCG TGCTGCTGCC CTTCCTCCAA AACCTGTTCA GCCTCCCTCT 
GTCTCTCCTC CTCTTCCTCC TCCTCCTCCG CAGCCCCACC ACCAGCCTTC TGTGCATATC 
CATCATCACC ACCACCATGC ACAGCCCCCT CATGCTCTTC TGGAAGATGA TGGGCCTCCC 
TCTCCTCACA CAGGTCTCCC TTCCTACCTG CAACAGCTTC AGAAGCCACA GCAACCTCCT 
ACTCAGTCTC CTATTCATTC ACTTCTCACA TCTGTCAAGG TGCAGTCCCA AGCACCCATG 
GCTGCTCCTC CCCAATCCAT GAGGCACCTC CAGCCTCTGG TTTACCCTGC TCCATCTTCC 
ACTGCTACCA CAGCGCCTCC TCCTGCCTCT TCTCACATAC ACCAACTGCA GTCTTCCCCT 
GCAGTCCCTC AGCAACAGCC AGCTGGTCAG GCACCGCCAC CCCCCCAGCA GCAGCAGCAC 
CCGGCCCTGC AAGGCGCGCT CGTTTCCTCA CATCCGCAGC ATGTGCAGCA CCAACAGGCC 
AAACAACAAC AAGTGATCCA GCATCATCAT CCTTCCCCAC GGCAGCAGAA GCAAGAGACA 
TATGCTGGAG GTCACTTGCG TGATGCTCCT TCCCCTCTCC TCCTGCATTC GCCTCAGGTT 
CCTCCCTATC CGGGTCTGGC TCACCCACCT TCCCCACAGT CTGTGCAACC TAAAAAGCAG 
GAGATGAGAG GAGCGTCGGT GCTGCAGCCT CAGCCGCTGG TCATGAAAGA AGATAAACGT 
CATTCGCCTT CACTGCGCCC AGAGGGCTTC TCTCCAGGAA TGCGCCCTGA ACCTCAGAAA 
GTTCCGGAGG TTCTAAAAGG ATCTAGTCAC ATCCAGCCAC GACCAGACTT GAAGCCAATG 
GATGTTGGAC GTCCCATACG GCCGACTGAC CAGAGCCTCC CACCTCAAGG GATGCCAGAA 
AAAGAGAAGC AAAAGCAAGA ACCTAAGACA CCTGTGGCTC CTAAAAAGGA CATAAAGATT 
AAAAACATGG GTTCGTGGGC TGGTCTAATG CAGAAGCCAC CAGTAACCCC AACTTCTGCG 
GGAAAGTCAA CAAGTGACAG CTTTGAGCTG TTCCGGAGGC AGGCCCGCGA GAAAGAGGAG 
CGCGAGAGGG CCCTCAAACT TCAGGCCGAA CAAGCAGAGA GAATGCGCAG GGAGCAGGAG 
AGAATGAGGT CCCGGGATGA AGATGACGCG CAGGAGCAGG CTAGGAAAGC CCACGAGGAG 
GCACGCCGGC GGCAGGAACA ACAGCAACAG CAGCAACAAC AACAGCAGCA ACAGCAACAC 
GTGCAGAGCA ACCTCCCCGC CGCTCCGTCA CCTGCGCAGA GCTCTCAGCC AATCATGGAT 
CAGCGAGAAA TGGCTAGAAA GCGGGAACAG GAGAGGAGAC GAAGGCAGGC GATGGCGCCC 
TCTATAGATA TGAATTTTCA GAGTGAACTG ATGGAGATAT TTGAACAGAA CCTGTTCTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947469.1
CDS	88..4290
Translation	MRMTSRYVAAALALCEEGAQGANARSPGSACDGSYAMSTETGPGTRLRATSGMGDGVEGAQMSGQQLPAAPQPQPQNSSLLNPPPPDIARPSQAKRQTNQLQYLLKTVLKTLWKHQFAWPFQLPVDVVKLNLPDYHKIIKNPMDMGTIKKRLENHYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKISEMPQEETELAVVQSKGRGRGRKEQSEQDTSITPMRTRVLSGSLGDKSAVKPPPVTPVSKPATPTPPTVTRAPTPPQTRPPQQARPPAITQAPISFSPTISQDVVVPTTVAPTPVPQPLPNHPAVIHTAAQPAKTKKGVKRKADTTTPTTHDPLHESSPLPSDSKPPKAGPRKESGRQIRPTKKTEVPDSQLPAPPVLQPQPTPTAEKDSKTSEQLRYCAGIIREMFSKKHQAYAWPFYKPVDVDALGLHDYREIIKHPMDLGTIKVKMDNWDYKDAKEFASDVRLMFSNCYKYNPPDHEVVIMARKLQDVFEMRYAKMPDEPEEAPAPAPSPAPGPPAPSIKGPPPTSSDSSSDSSSDSESSSDSEEERAQRLAELQEQLKAVHEQLAALSQPQPNKPKKKEREKRKEKHKRKEEVEEPRKGRIREPPAKKPKKSVQGSGGTPSIKKEAPPPVARPARPVPPPAPCESSEEETQRCRPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQDKIEAPTSGTGKVKGYSSSESESSSESSTSDSEESDPETAPNQKKKGHSGRESRKHHHPMQQPLAAPPPVMKPPSPTLAPPYPPPSSLDSSHPSLHHPLHSANVFDAVMQLPPDLPPHLTGQTEHCSPPHLNQHALTSPPALHNAMPQQPSRPSNRAAALPPKPVQPPSVSPPLPPPPPQPHHQPSVHIHHHHHHAQPPHALLEDDGPPSPHTGLPSYLQQLQKPQQPPTQSPIHSLLTSVKVQSQAPMAAPPQSMRHLQPLVYPAPSSTATTAPPPASSHIHQLQSSPAVPQQQPAGQAPPPPQQQQHPALQGALVSSHPQHVQHQQAKQQQVIQHHHPSPRQQKQETYAGGHLRDAPSPLLLHSPQVPPYPGLAHPPSPQSVQPKKQEMRGASVLQPQPLVMKEDKRHSPSLRPEGFSPGMRPEPQKVPEVLKGSSHIQPRPDLKPMDVGRPIRPTDQSLPPQGMPEKEKQKQEPKTPVAPKKDIKIKNMGSWAGLMQKPPVTPTSAGKSTSDSFELFRRQAREKEERERALKLQAEQAERMRREQERMRSRDEDDAQEQARKAHEEARRRQEQQQQQQQQQQQQQHVQSNLPAAPSPAQSSQPIMDQREMARKREQERRRRQAMAPSIDMNFQSELMEIFEQNLFS

Target ORF information:

RefSeq Version	XM_018091980.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091980.2

ORF Insert Sequence:

ATGCGCATGA CGTCACGGTA CGTAGCGGCG GCGCTGGCGC TGTGCGAGGA AGGAGCCCAG 
GGAGCGAACG CTCGGTCACC TGGCTCTGCG TGCGATGGGA GCTACGCTAT GTCAACCGAG 
ACTGGGCCTG GTACACGTCT GAGAGCCACA TCTGGAATGG GTGACGGAGT AGAAGGGGCT 
CAGATGTCGG GCCAACAGCT GCCTGCCGCT CCCCAGCCCC AGCCACAGAA CTCATCGCTG 
CTCAATCCTC CTCCTCCTGA TATCGCAAGA CCCAGCCAAG CAAAGCGCCA GACCAACCAG 
CTGCAGTACT TACTTAAGAC AGTGCTCAAG ACATTGTGGA AGCACCAGTT TGCATGGCCT 
TTCCAGCTAC CTGTTGATGT GGTCAAGCTC AATCTGCCTG ATTATCATAA AATAATTAAA 
AACCCAATGG ACATGGGCAC CATTAAAAAG CGCCTCGAGA ACCATTATTA CTGGAACGCC 
CAAGAATGTA TCCAGGACTT CAACACAATG TTTACAAACT GCTACATCTA TAACAAGCCT 
GGAGATGACA TAGTGCTGAT GGCCGAAGCC CTTGAGAAGC TTTTTCTGCA GAAGATCTCA 
GAGATGCCCC AGGAAGAAAC CGAACTGGCA GTTGTGCAGA GCAAAGGCCG TGGCCGGGGA 
AGAAAGGAGC AAAGTGAGCA AGATACTTCA ATCACCCCCA TGAGAACCCG AGTCCTGTCT 
GGGTCACTAG GAGACAAGAG CGCCGTCAAA CCTCCCCCTG TTACACCTGT GTCCAAGCCT 
GCAACCCCAA CGCCCCCTAC TGTGACCAGG GCTCCCACCC CTCCACAAAC CAGACCACCC 
CAACAAGCTC GCCCTCCAGC TATCACTCAA GCCCCCATCA GTTTCTCTCC CACCATCAGT 
CAGGATGTGG TGGTTCCAAC GACCGTAGCC CCAACACCAG TTCCTCAGCC ACTTCCAAAT 
CATCCTGCAG TCATCCACAC TGCTGCCCAG CCTGCAAAGA CAAAGAAAGG TGTGAAGAGG 
AAAGCAGACA CCACAACCCC CACTACCCAT GACCCGCTCC ATGAGTCCTC CCCACTCCCT 
AGTGACTCCA AGCCTCCAAA GGCTGGCCCT CGAAAGGAGA GTGGGCGTCA GATTCGACCA 
ACTAAGAAAA CCGAGGTGCC TGACTCTCAA CTTCCTGCCC CCCCTGTTTT GCAACCCCAA 
CCTACACCCA CTGCTGAGAA GGACAGCAAG ACAAGCGAGC AGTTACGGTA TTGTGCAGGC 
ATTATCCGGG AAATGTTTTC CAAAAAGCAT CAGGCCTATG CGTGGCCATT TTACAAGCCC 
GTTGATGTGG ATGCCCTTGG GTTACATGAC TACCGTGAAA TTATCAAGCA TCCAATGGAC 
CTAGGCACCA TTAAGGTAAA GATGGATAAT TGGGACTACA AGGACGCAAA GGAATTTGCA 
TCTGATGTGC GGCTTATGTT CTCAAACTGT TACAAGTACA ATCCCCCTGA TCATGAAGTT 
GTGATAATGG CTCGCAAGTT GCAGGATGTT TTTGAAATGC GCTATGCCAA AATGCCTGAT 
GAACCTGAGG AGGCGCCAGC TCCTGCACCC TCTCCTGCTC CAGGGCCCCC AGCCCCATCC 
ATCAAAGGCC CTCCGCCAAC CTCTAGTGAC AGCAGCAGCG ATAGCTCATC TGATAGTGAA 
AGCAGTTCAG ACAGTGAGGA GGAACGGGCA CAAAGACTGG CAGAGCTTCA GGAACAGTTG 
AAAGCTGTAC ATGAGCAGTT GGCAGCTCTA TCTCAGCCAC AGCCGAACAA ACCAAAGAAG 
AAAGAGCGTG AAAAGCGGAA GGAAAAGCAC AAACGAAAAG AAGAGGTGGA GGAGCCTAGA 
AAGGGCCGTA TCCGAGAGCC ACCAGCCAAG AAACCCAAGA AAAGTGTTCA AGGGTCCGGA 
GGTACACCAA GCATCAAGAA AGAAGCACCT CCCCCTGTGG CACGCCCTGC TCGTCCAGTG 
CCACCTCCGG CACCATGTGA GTCCTCTGAG GAAGAAACGC AACGATGTCG GCCAATGTCA 
TACGAAGAGA AACGCCAGCT AAGCTTGGAC ATTAATAAGC TGCCAGGCGA AAAACTGGGC 
CGCGTCGTGC ACATCATCCA GTCTCGCGAG CCTTCCCTGA AGAACTCCAA TCCAGATGAG 
ATTGAGATAG ACTTTGAGAC ACTTAAACCG TCCACACTGC GAGAGTTGGA GAGATATGTT 
ACCTCCTGTT TGAGAAAGAA GCGAAAACCA CAGGATAAAA TAGAAGCTCC CACATCTGGC 
ACTGGTAAAG TAAAAGGCTA TTCCTCTTCA GAGTCTGAGA GCTCAAGTGA GTCCAGCACG 
TCAGACAGCG AGGAGTCCGA CCCAGAAACA GCCCCAAATC AGAAGAAAAA GGGGCACTCG 
GGGAGAGAGT CTAGAAAGCA TCATCACCCT ATGCAACAGC CTCTTGCCGC ACCACCCCCT 
GTCATGAAGC CTCCGTCTCC CACCTTAGCA CCACCTTACC CTCCGCCTTC CAGCCTGGAC 
TCCTCACATC CTTCTCTCCA CCACCCTCTC CACTCTGCGA ATGTTTTTGA TGCAGTCATG 
CAGCTTCCTC CTGATCTGCC TCCTCACCTT ACTGGTCAGA CAGAGCACTG CTCGCCCCCA 
CACCTCAACC AACATGCCCT GACAAGCCCT CCAGCCCTAC ACAACGCAAT GCCCCAGCAG 
CCCTCCAGGC CATCCAACCG TGCTGCTGCC CTTCCTCCAA AACCTGTTCA GCCTCCCTCT 
GTCTCTCCTC CTCTTCCTCC TCCTCCTCCG CAGCCCCACC ACCAGCCTTC TGTGCATATC 
CATCATCACC ACCACCATGC ACAGCCCCCT CATGCTCTTC TGGAAGATGA TGGGCCTCCC 
TCTCCTCACA CAGGTCTCCC TTCCTACCTG CAACAGCTTC AGAAGCCACA GCAACCTCCT 
ACTCAGTCTC CTATTCATTC ACTTCTCACA TCTGTCAAGG TGCAGTCCCA AGCACCCATG 
GCTGCTCCTC CCCAATCCAT GAGGCACCTC CAGCCTCTGG TTTACCCTGC TCCATCTTCC 
ACTGCTACCA CAGCGCCTCC TCCTGCCTCT TCTCACATAC ACCAACTGCA GTCTTCCCCT 
GCAGTCCCTC AGCAACAGCC AGCTGGTCAG GCACCGCCAC CCCCCCAGCA GCAGCAGCAC 
CCGGCCCTGC AAGGCGCGCT CGTTTCCTCA CATCCGCAGC ATGTGCAGCA CCAACAGGCC 
AAACAACAAC AAGTGATCCA GCATCATCAT CCTTCCCCAC GGCAGCAGAA GCAAGAGACA 
TATGCTGGAG GTCACTTGCG TGATGCTCCT TCCCCTCTCC TCCTGCATTC GCCTCAGGTT 
CCTCCCTATC CGGGTCTGGC TCACCCACCT TCCCCACAGT CTGTGCAACC TAAAAAGCAG 
GAGATGAGAG GAGCGTCGGT GCTGCAGCCT CAGCCGCTGG TCATGAAAGA AGATAAACGT 
CATTCGCCTT CACTGCGCCC AGAGGGCTTC TCTCCAGGAA TGCGCCCTGA ACCTCAGAAA 
GTTCCGGAGG TTCTAAAAGG ATCTAGTCAC ATCCAGCCAC GACCAGACTT GAAGCCAATG 
GATGTTGGAC GTCCCATACG GCCGACTGAC CAGAGCCTCC CACCTCAAGG GATGCCAGAA 
AAAGAGAAGC AAAAGCAAGA ACCTAAGACA CCTGTGGCTC CTAAAAAGGA CATAAAGATT 
AAAAACATGG GTTCGTGGGC TGGTCTAATG CAGAAGCCAC CAGTAACCCC AACTTCTGCG 
GGAAAGTCAA CAAGTGACAG CTTTGAGCTG TTCCGGAGGC AGGCCCGCGA GAAAGAGGAG 
CGCGAGAGGG CCCTCAAACT TCAGGCCGAA CAAGCAGAGA GAATGCGCAG GGAGCAGGAG 
AGAATGAGGT CCCGGGATGA AGATGACGCG CAGGAGCAGG CTAGGAAAGC CCACGAGGAG 
GCACGCCGGC GGCAGGAACA ACAGCAACAG CAGCAACAAC AACAGCAGCA ACAGCAACAC 
GTGCAGAGCA ACCTCCCCGC CGCTCCGTCA CCTGCGCAGA GCTCTCAGCC AATCATGGAT 
CAGCGAGAAA TGGCTAGAAA GCGGGAACAG GAGAGGAGAC GAAGGCAGGC GATGGCGCCC 
TCTATAGATA TGAATTTTCA GAGTGAACTG ATGGAGATAT TTGAACAGAA CCTGTTCTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brd4 ( XM_018091980.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947469.1 Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X1, mRNA.

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CloneID	OXa43062
Clone ID Related Accession (Same CDS sequence)	XM_018091980.2 , XM_018091980.1
Accession Version	XM_018091980.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCATGA CGTCACGGTA CGTAGCGGCG GCGCTGGCGC TGTGCGAGGA AGGAGCCCAG 
GGAGCGAACG CTCGGTCACC TGGCTCTGCG TGCGATGGGA GCTACGCTAT GTCAACCGAG 
ACTGGGCCTG GTACACGTCT GAGAGCCACA TCTGGAATGG GTGACGGAGT AGAAGGGGCT 
CAGATGTCGG GCCAACAGCT GCCTGCCGCT CCCCAGCCCC AGCCACAGAA CTCATCGCTG 
CTCAATCCTC CTCCTCCTGA TATCGCAAGA CCCAGCCAAG CAAAGCGCCA GACCAACCAG 
CTGCAGTACT TACTTAAGAC AGTGCTCAAG ACATTGTGGA AGCACCAGTT TGCATGGCCT 
TTCCAGCTAC CTGTTGATGT GGTCAAGCTC AATCTGCCTG ATTATCATAA AATAATTAAA 
AACCCAATGG ACATGGGCAC CATTAAAAAG CGCCTCGAGA ACCATTATTA CTGGAACGCC 
CAAGAATGTA TCCAGGACTT CAACACAATG TTTACAAACT GCTACATCTA TAACAAGCCT 
GGAGATGACA TAGTGCTGAT GGCCGAAGCC CTTGAGAAGC TTTTTCTGCA GAAGATCTCA 
GAGATGCCCC AGGAAGAAAC CGAACTGGCA GTTGTGCAGA GCAAAGGCCG TGGCCGGGGA 
AGAAAGGAGC AAAGTGAGCA AGATACTTCA ATCACCCCCA TGAGAACCCG AGTCCTGTCT 
GGGTCACTAG GAGACAAGAG CGCCGTCAAA CCTCCCCCTG TTACACCTGT GTCCAAGCCT 
GCAACCCCAA CGCCCCCTAC TGTGACCAGG GCTCCCACCC CTCCACAAAC CAGACCACCC 
CAACAAGCTC GCCCTCCAGC TATCACTCAA GCCCCCATCA GTTTCTCTCC CACCATCAGT 
CAGGATGTGG TGGTTCCAAC GACCGTAGCC CCAACACCAG TTCCTCAGCC ACTTCCAAAT 
CATCCTGCAG TCATCCACAC TGCTGCCCAG CCTGCAAAGA CAAAGAAAGG TGTGAAGAGG 
AAAGCAGACA CCACAACCCC CACTACCCAT GACCCGCTCC ATGAGTCCTC CCCACTCCCT 
AGTGACTCCA AGCCTCCAAA GGCTGGCCCT CGAAAGGAGA GTGGGCGTCA GATTCGACCA 
ACTAAGAAAA CCGAGGTGCC TGACTCTCAA CTTCCTGCCC CCCCTGTTTT GCAACCCCAA 
CCTACACCCA CTGCTGAGAA GGACAGCAAG ACAAGCGAGC AGTTACGGTA TTGTGCAGGC 
ATTATCCGGG AAATGTTTTC CAAAAAGCAT CAGGCCTATG CGTGGCCATT TTACAAGCCC 
GTTGATGTGG ATGCCCTTGG GTTACATGAC TACCGTGAAA TTATCAAGCA TCCAATGGAC 
CTAGGCACCA TTAAGGTAAA GATGGATAAT TGGGACTACA AGGACGCAAA GGAATTTGCA 
TCTGATGTGC GGCTTATGTT CTCAAACTGT TACAAGTACA ATCCCCCTGA TCATGAAGTT 
GTGATAATGG CTCGCAAGTT GCAGGATGTT TTTGAAATGC GCTATGCCAA AATGCCTGAT 
GAACCTGAGG AGGCGCCAGC TCCTGCACCC TCTCCTGCTC CAGGGCCCCC AGCCCCATCC 
ATCAAAGGCC CTCCGCCAAC CTCTAGTGAC AGCAGCAGCG ATAGCTCATC TGATAGTGAA 
AGCAGTTCAG ACAGTGAGGA GGAACGGGCA CAAAGACTGG CAGAGCTTCA GGAACAGTTG 
AAAGCTGTAC ATGAGCAGTT GGCAGCTCTA TCTCAGCCAC AGCCGAACAA ACCAAAGAAG 
AAAGAGCGTG AAAAGCGGAA GGAAAAGCAC AAACGAAAAG AAGAGGTGGA GGAGCCTAGA 
AAGGGCCGTA TCCGAGAGCC ACCAGCCAAG AAACCCAAGA AAAGTGTTCA AGGGTCCGGA 
GGTACACCAA GCATCAAGAA AGAAGCACCT CCCCCTGTGG CACGCCCTGC TCGTCCAGTG 
CCACCTCCGG CACCATGTGA GTCCTCTGAG GAAGAAACGC AACGATGTCG GCCAATGTCA 
TACGAAGAGA AACGCCAGCT AAGCTTGGAC ATTAATAAGC TGCCAGGCGA AAAACTGGGC 
CGCGTCGTGC ACATCATCCA GTCTCGCGAG CCTTCCCTGA AGAACTCCAA TCCAGATGAG 
ATTGAGATAG ACTTTGAGAC ACTTAAACCG TCCACACTGC GAGAGTTGGA GAGATATGTT 
ACCTCCTGTT TGAGAAAGAA GCGAAAACCA CAGGATAAAA TAGAAGCTCC CACATCTGGC 
ACTGGTAAAG TAAAAGGCTA TTCCTCTTCA GAGTCTGAGA GCTCAAGTGA GTCCAGCACG 
TCAGACAGCG AGGAGTCCGA CCCAGAAACA GCCCCAAATC AGAAGAAAAA GGGGCACTCG 
GGGAGAGAGT CTAGAAAGCA TCATCACCCT ATGCAACAGC CTCTTGCCGC ACCACCCCCT 
GTCATGAAGC CTCCGTCTCC CACCTTAGCA CCACCTTACC CTCCGCCTTC CAGCCTGGAC 
TCCTCACATC CTTCTCTCCA CCACCCTCTC CACTCTGCGA ATGTTTTTGA TGCAGTCATG 
CAGCTTCCTC CTGATCTGCC TCCTCACCTT ACTGGTCAGA CAGAGCACTG CTCGCCCCCA 
CACCTCAACC AACATGCCCT GACAAGCCCT CCAGCCCTAC ACAACGCAAT GCCCCAGCAG 
CCCTCCAGGC CATCCAACCG TGCTGCTGCC CTTCCTCCAA AACCTGTTCA GCCTCCCTCT 
GTCTCTCCTC CTCTTCCTCC TCCTCCTCCG CAGCCCCACC ACCAGCCTTC TGTGCATATC 
CATCATCACC ACCACCATGC ACAGCCCCCT CATGCTCTTC TGGAAGATGA TGGGCCTCCC 
TCTCCTCACA CAGGTCTCCC TTCCTACCTG CAACAGCTTC AGAAGCCACA GCAACCTCCT 
ACTCAGTCTC CTATTCATTC ACTTCTCACA TCTGTCAAGG TGCAGTCCCA AGCACCCATG 
GCTGCTCCTC CCCAATCCAT GAGGCACCTC CAGCCTCTGG TTTACCCTGC TCCATCTTCC 
ACTGCTACCA CAGCGCCTCC TCCTGCCTCT TCTCACATAC ACCAACTGCA GTCTTCCCCT 
GCAGTCCCTC AGCAACAGCC AGCTGGTCAG GCACCGCCAC CCCCCCAGCA GCAGCAGCAC 
CCGGCCCTGC AAGGCGCGCT CGTTTCCTCA CATCCGCAGC ATGTGCAGCA CCAACAGGCC 
AAACAACAAC AAGTGATCCA GCATCATCAT CCTTCCCCAC GGCAGCAGAA GCAAGAGACA 
TATGCTGGAG GTCACTTGCG TGATGCTCCT TCCCCTCTCC TCCTGCATTC GCCTCAGGTT 
CCTCCCTATC CGGGTCTGGC TCACCCACCT TCCCCACAGT CTGTGCAACC TAAAAAGCAG 
GAGATGAGAG GAGCGTCGGT GCTGCAGCCT CAGCCGCTGG TCATGAAAGA AGATAAACGT 
CATTCGCCTT CACTGCGCCC AGAGGGCTTC TCTCCAGGAA TGCGCCCTGA ACCTCAGAAA 
GTTCCGGAGG TTCTAAAAGG ATCTAGTCAC ATCCAGCCAC GACCAGACTT GAAGCCAATG 
GATGTTGGAC GTCCCATACG GCCGACTGAC CAGAGCCTCC CACCTCAAGG GATGCCAGAA 
AAAGAGAAGC AAAAGCAAGA ACCTAAGACA CCTGTGGCTC CTAAAAAGGA CATAAAGATT 
AAAAACATGG GTTCGTGGGC TGGTCTAATG CAGAAGCCAC CAGTAACCCC AACTTCTGCG 
GGAAAGTCAA CAAGTGACAG CTTTGAGCTG TTCCGGAGGC AGGCCCGCGA GAAAGAGGAG 
CGCGAGAGGG CCCTCAAACT TCAGGCCGAA CAAGCAGAGA GAATGCGCAG GGAGCAGGAG 
AGAATGAGGT CCCGGGATGA AGATGACGCG CAGGAGCAGG CTAGGAAAGC CCACGAGGAG 
GCACGCCGGC GGCAGGAACA ACAGCAACAG CAGCAACAAC AACAGCAGCA ACAGCAACAC 
GTGCAGAGCA ACCTCCCCGC CGCTCCGTCA CCTGCGCAGA GCTCTCAGCC AATCATGGAT 
CAGCGAGAAA TGGCTAGAAA GCGGGAACAG GAGAGGAGAC GAAGGCAGGC GATGGCGCCC 
TCTATAGATA TGAATTTTCA GAGTGAACTG ATGGAGATAT TTGAACAGAA CCTGTTCTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947469.1
CDS	82..4284
Translation	MRMTSRYVAAALALCEEGAQGANARSPGSACDGSYAMSTETGPGTRLRATSGMGDGVEGAQMSGQQLPAAPQPQPQNSSLLNPPPPDIARPSQAKRQTNQLQYLLKTVLKTLWKHQFAWPFQLPVDVVKLNLPDYHKIIKNPMDMGTIKKRLENHYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKISEMPQEETELAVVQSKGRGRGRKEQSEQDTSITPMRTRVLSGSLGDKSAVKPPPVTPVSKPATPTPPTVTRAPTPPQTRPPQQARPPAITQAPISFSPTISQDVVVPTTVAPTPVPQPLPNHPAVIHTAAQPAKTKKGVKRKADTTTPTTHDPLHESSPLPSDSKPPKAGPRKESGRQIRPTKKTEVPDSQLPAPPVLQPQPTPTAEKDSKTSEQLRYCAGIIREMFSKKHQAYAWPFYKPVDVDALGLHDYREIIKHPMDLGTIKVKMDNWDYKDAKEFASDVRLMFSNCYKYNPPDHEVVIMARKLQDVFEMRYAKMPDEPEEAPAPAPSPAPGPPAPSIKGPPPTSSDSSSDSSSDSESSSDSEEERAQRLAELQEQLKAVHEQLAALSQPQPNKPKKKEREKRKEKHKRKEEVEEPRKGRIREPPAKKPKKSVQGSGGTPSIKKEAPPPVARPARPVPPPAPCESSEEETQRCRPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQDKIEAPTSGTGKVKGYSSSESESSSESSTSDSEESDPETAPNQKKKGHSGRESRKHHHPMQQPLAAPPPVMKPPSPTLAPPYPPPSSLDSSHPSLHHPLHSANVFDAVMQLPPDLPPHLTGQTEHCSPPHLNQHALTSPPALHNAMPQQPSRPSNRAAALPPKPVQPPSVSPPLPPPPPQPHHQPSVHIHHHHHHAQPPHALLEDDGPPSPHTGLPSYLQQLQKPQQPPTQSPIHSLLTSVKVQSQAPMAAPPQSMRHLQPLVYPAPSSTATTAPPPASSHIHQLQSSPAVPQQQPAGQAPPPPQQQQHPALQGALVSSHPQHVQHQQAKQQQVIQHHHPSPRQQKQETYAGGHLRDAPSPLLLHSPQVPPYPGLAHPPSPQSVQPKKQEMRGASVLQPQPLVMKEDKRHSPSLRPEGFSPGMRPEPQKVPEVLKGSSHIQPRPDLKPMDVGRPIRPTDQSLPPQGMPEKEKQKQEPKTPVAPKKDIKIKNMGSWAGLMQKPPVTPTSAGKSTSDSFELFRRQAREKEERERALKLQAEQAERMRREQERMRSRDEDDAQEQARKAHEEARRRQEQQQQQQQQQQQQQHVQSNLPAAPSPAQSSQPIMDQREMARKREQERRRRQAMAPSIDMNFQSELMEIFEQNLFS

Target ORF information:

RefSeq Version	XM_018091980.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091980.1

ORF Insert Sequence:

ATGCGCATGA CGTCACGGTA CGTAGCGGCG GCGCTGGCGC TGTGCGAGGA AGGAGCCCAG 
GGAGCGAACG CTCGGTCACC TGGCTCTGCG TGCGATGGGA GCTACGCTAT GTCAACCGAG 
ACTGGGCCTG GTACACGTCT GAGAGCCACA TCTGGAATGG GTGACGGAGT AGAAGGGGCT 
CAGATGTCGG GCCAACAGCT GCCTGCCGCT CCCCAGCCCC AGCCACAGAA CTCATCGCTG 
CTCAATCCTC CTCCTCCTGA TATCGCAAGA CCCAGCCAAG CAAAGCGCCA GACCAACCAG 
CTGCAGTACT TACTTAAGAC AGTGCTCAAG ACATTGTGGA AGCACCAGTT TGCATGGCCT 
TTCCAGCTAC CTGTTGATGT GGTCAAGCTC AATCTGCCTG ATTATCATAA AATAATTAAA 
AACCCAATGG ACATGGGCAC CATTAAAAAG CGCCTCGAGA ACCATTATTA CTGGAACGCC 
CAAGAATGTA TCCAGGACTT CAACACAATG TTTACAAACT GCTACATCTA TAACAAGCCT 
GGAGATGACA TAGTGCTGAT GGCCGAAGCC CTTGAGAAGC TTTTTCTGCA GAAGATCTCA 
GAGATGCCCC AGGAAGAAAC CGAACTGGCA GTTGTGCAGA GCAAAGGCCG TGGCCGGGGA 
AGAAAGGAGC AAAGTGAGCA AGATACTTCA ATCACCCCCA TGAGAACCCG AGTCCTGTCT 
GGGTCACTAG GAGACAAGAG CGCCGTCAAA CCTCCCCCTG TTACACCTGT GTCCAAGCCT 
GCAACCCCAA CGCCCCCTAC TGTGACCAGG GCTCCCACCC CTCCACAAAC CAGACCACCC 
CAACAAGCTC GCCCTCCAGC TATCACTCAA GCCCCCATCA GTTTCTCTCC CACCATCAGT 
CAGGATGTGG TGGTTCCAAC GACCGTAGCC CCAACACCAG TTCCTCAGCC ACTTCCAAAT 
CATCCTGCAG TCATCCACAC TGCTGCCCAG CCTGCAAAGA CAAAGAAAGG TGTGAAGAGG 
AAAGCAGACA CCACAACCCC CACTACCCAT GACCCGCTCC ATGAGTCCTC CCCACTCCCT 
AGTGACTCCA AGCCTCCAAA GGCTGGCCCT CGAAAGGAGA GTGGGCGTCA GATTCGACCA 
ACTAAGAAAA CCGAGGTGCC TGACTCTCAA CTTCCTGCCC CCCCTGTTTT GCAACCCCAA 
CCTACACCCA CTGCTGAGAA GGACAGCAAG ACAAGCGAGC AGTTACGGTA TTGTGCAGGC 
ATTATCCGGG AAATGTTTTC CAAAAAGCAT CAGGCCTATG CGTGGCCATT TTACAAGCCC 
GTTGATGTGG ATGCCCTTGG GTTACATGAC TACCGTGAAA TTATCAAGCA TCCAATGGAC 
CTAGGCACCA TTAAGGTAAA GATGGATAAT TGGGACTACA AGGACGCAAA GGAATTTGCA 
TCTGATGTGC GGCTTATGTT CTCAAACTGT TACAAGTACA ATCCCCCTGA TCATGAAGTT 
GTGATAATGG CTCGCAAGTT GCAGGATGTT TTTGAAATGC GCTATGCCAA AATGCCTGAT 
GAACCTGAGG AGGCGCCAGC TCCTGCACCC TCTCCTGCTC CAGGGCCCCC AGCCCCATCC 
ATCAAAGGCC CTCCGCCAAC CTCTAGTGAC AGCAGCAGCG ATAGCTCATC TGATAGTGAA 
AGCAGTTCAG ACAGTGAGGA GGAACGGGCA CAAAGACTGG CAGAGCTTCA GGAACAGTTG 
AAAGCTGTAC ATGAGCAGTT GGCAGCTCTA TCTCAGCCAC AGCCGAACAA ACCAAAGAAG 
AAAGAGCGTG AAAAGCGGAA GGAAAAGCAC AAACGAAAAG AAGAGGTGGA GGAGCCTAGA 
AAGGGCCGTA TCCGAGAGCC ACCAGCCAAG AAACCCAAGA AAAGTGTTCA AGGGTCCGGA 
GGTACACCAA GCATCAAGAA AGAAGCACCT CCCCCTGTGG CACGCCCTGC TCGTCCAGTG 
CCACCTCCGG CACCATGTGA GTCCTCTGAG GAAGAAACGC AACGATGTCG GCCAATGTCA 
TACGAAGAGA AACGCCAGCT AAGCTTGGAC ATTAATAAGC TGCCAGGCGA AAAACTGGGC 
CGCGTCGTGC ACATCATCCA GTCTCGCGAG CCTTCCCTGA AGAACTCCAA TCCAGATGAG 
ATTGAGATAG ACTTTGAGAC ACTTAAACCG TCCACACTGC GAGAGTTGGA GAGATATGTT 
ACCTCCTGTT TGAGAAAGAA GCGAAAACCA CAGGATAAAA TAGAAGCTCC CACATCTGGC 
ACTGGTAAAG TAAAAGGCTA TTCCTCTTCA GAGTCTGAGA GCTCAAGTGA GTCCAGCACG 
TCAGACAGCG AGGAGTCCGA CCCAGAAACA GCCCCAAATC AGAAGAAAAA GGGGCACTCG 
GGGAGAGAGT CTAGAAAGCA TCATCACCCT ATGCAACAGC CTCTTGCCGC ACCACCCCCT 
GTCATGAAGC CTCCGTCTCC CACCTTAGCA CCACCTTACC CTCCGCCTTC CAGCCTGGAC 
TCCTCACATC CTTCTCTCCA CCACCCTCTC CACTCTGCGA ATGTTTTTGA TGCAGTCATG 
CAGCTTCCTC CTGATCTGCC TCCTCACCTT ACTGGTCAGA CAGAGCACTG CTCGCCCCCA 
CACCTCAACC AACATGCCCT GACAAGCCCT CCAGCCCTAC ACAACGCAAT GCCCCAGCAG 
CCCTCCAGGC CATCCAACCG TGCTGCTGCC CTTCCTCCAA AACCTGTTCA GCCTCCCTCT 
GTCTCTCCTC CTCTTCCTCC TCCTCCTCCG CAGCCCCACC ACCAGCCTTC TGTGCATATC 
CATCATCACC ACCACCATGC ACAGCCCCCT CATGCTCTTC TGGAAGATGA TGGGCCTCCC 
TCTCCTCACA CAGGTCTCCC TTCCTACCTG CAACAGCTTC AGAAGCCACA GCAACCTCCT 
ACTCAGTCTC CTATTCATTC ACTTCTCACA TCTGTCAAGG TGCAGTCCCA AGCACCCATG 
GCTGCTCCTC CCCAATCCAT GAGGCACCTC CAGCCTCTGG TTTACCCTGC TCCATCTTCC 
ACTGCTACCA CAGCGCCTCC TCCTGCCTCT TCTCACATAC ACCAACTGCA GTCTTCCCCT 
GCAGTCCCTC AGCAACAGCC AGCTGGTCAG GCACCGCCAC CCCCCCAGCA GCAGCAGCAC 
CCGGCCCTGC AAGGCGCGCT CGTTTCCTCA CATCCGCAGC ATGTGCAGCA CCAACAGGCC 
AAACAACAAC AAGTGATCCA GCATCATCAT CCTTCCCCAC GGCAGCAGAA GCAAGAGACA 
TATGCTGGAG GTCACTTGCG TGATGCTCCT TCCCCTCTCC TCCTGCATTC GCCTCAGGTT 
CCTCCCTATC CGGGTCTGGC TCACCCACCT TCCCCACAGT CTGTGCAACC TAAAAAGCAG 
GAGATGAGAG GAGCGTCGGT GCTGCAGCCT CAGCCGCTGG TCATGAAAGA AGATAAACGT 
CATTCGCCTT CACTGCGCCC AGAGGGCTTC TCTCCAGGAA TGCGCCCTGA ACCTCAGAAA 
GTTCCGGAGG TTCTAAAAGG ATCTAGTCAC ATCCAGCCAC GACCAGACTT GAAGCCAATG 
GATGTTGGAC GTCCCATACG GCCGACTGAC CAGAGCCTCC CACCTCAAGG GATGCCAGAA 
AAAGAGAAGC AAAAGCAAGA ACCTAAGACA CCTGTGGCTC CTAAAAAGGA CATAAAGATT 
AAAAACATGG GTTCGTGGGC TGGTCTAATG CAGAAGCCAC CAGTAACCCC AACTTCTGCG 
GGAAAGTCAA CAAGTGACAG CTTTGAGCTG TTCCGGAGGC AGGCCCGCGA GAAAGAGGAG 
CGCGAGAGGG CCCTCAAACT TCAGGCCGAA CAAGCAGAGA GAATGCGCAG GGAGCAGGAG 
AGAATGAGGT CCCGGGATGA AGATGACGCG CAGGAGCAGG CTAGGAAAGC CCACGAGGAG 
GCACGCCGGC GGCAGGAACA ACAGCAACAG CAGCAACAAC AACAGCAGCA ACAGCAACAC 
GTGCAGAGCA ACCTCCCCGC CGCTCCGTCA CCTGCGCAGA GCTCTCAGCC AATCATGGAT 
CAGCGAGAAA TGGCTAGAAA GCGGGAACAG GAGAGGAGAC GAAGGCAGGC GATGGCGCCC 
TCTATAGATA TGAATTTTCA GAGTGAACTG ATGGAGATAT TTGAACAGAA CCTGTTCTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brd4 ( XM_012953715.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012809169.2 Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X4, mRNA.

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CloneID	OXa43063
Clone ID Related Accession (Same CDS sequence)	XM_012953715.2
Accession Version	XM_012953715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain-containing protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030679.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012953715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCATGA CGTCACGGTA CGTAGCGGCG GCGCTGGCGC TGTGCGAGGA AGGAGCCCAG 
GGAGCGAACG CTCGGTCACC TGGCTCTGCG TGCGATGGGA GCTACGCTAT GTCAACCGAG 
ACTGGGCCTG GTACACGTCT GAGAGCCACA TCTGGAATGG GTGACGGAGT AGAAGGGGCT 
CAGATGTCGG GCCAACAGCT GCCTGCCGCT CCCCAGCCCC AGCCACAGAA CTCATCGCTG 
CTCAATCCTC CTCCTCCTGA TATCGCAAGA CCCAGCCAAG CAAAGCGCCA GACCAACCAG 
CTGCAGTACT TACTTAAGAC AGTGCTCAAG ACATTGTGGA AGCACCAGTT TGCATGGCCT 
TTCCAGCTAC CTGTTGATGT GGTCAAGCTC AATCTGCCTG ATTATCATAA AATAATTAAA 
AACCCAATGG ACATGGGCAC CATTAAAAAG CGCCTCGAGA ACCATTATTA CTGGAACGCC 
CAAGAATGTA TCCAGGACTT CAACACAATG TTTACAAACT GCTACATCTA TAACAAGCCT 
GGAGATGACA TAGTGCTGAT GGCCGAAGCC CTTGAGAAGC TTTTTCTGCA GAAGATCTCA 
GAGATGCCCC AGGAAGAAAC CGAACTGGCA GTTGTGCAGA GCAAAGGCCG TGGCCGGGGA 
AGAAAGGAGC AAAGTGAGCA AGATACTTCA ATCACCCCCA TGAGAACCCG AGTCCTGTCT 
GGGTCACTAG GAGACAAGAG CGCCGTCAAA CCTCCCCCTG TTACACCTGT GTCCAAGCCT 
GCAACCCCAA CGCCCCCTAC TGTGACCAGG GCTCCCACCC CTCCACAAAC CAGACCACCC 
CAACAAGCTC GCCCTCCAGC TATCACTCAA GCCCCCATCA GTTTCTCTCC CACCATCAGT 
CAGGATGTGG TGGTTCCAAC GACCGTAGCC CCAACACCAG TTCCTCAGCC ACTTCCAAAT 
CATCCTGCAG TCATCCACAC TGCTGCCCAG CCTGCAAAGA CAAAGAAAGG TGTGAAGAGG 
AAAGCAGACA CCACAACCCC CACTACCCAT GACCCGCTCC ATGAGTCCTC CCCACTCCCT 
AGTGACTCCA AGCCTCCAAA GGCTGGCCCT CGAAAGGAGA GTGGGCGTCA GATTCGACCA 
ACTAAGAAAA CCGAGGTGCC TGACTCTCAA CTTCCTGCCC CCCCTGTTTT GCAACCCCAA 
CCTACACCCA CTGCTGAGAA GGACAGCAAG ACAAGCGAGC AGTTACGGTA TTGTGCAGGC 
ATTATCCGGG AAATGTTTTC CAAAAAGCAT CAGGCCTATG CGTGGCCATT TTACAAGCCC 
GTTGATGTGG ATGCCCTTGG GTTACATGAC TACCGTGAAA TTATCAAGCA TCCAATGGAC 
CTAGGCACCA TTAAGGTAAA GATGGATAAT TGGGACTACA AGGACGCAAA GGAATTTGCA 
TCTGATGTGC GGCTTATGTT CTCAAACTGT TACAAGTACA ATCCCCCTGA TCATGAAGTT 
GTGATAATGG CTCGCAAGTT GCAGGATGTT TTTGAAATGC GCTATGCCAA AATGCCTGAT 
GAACCTGAGG AGGCGCCAGC TCCTGCACCC TCTCCTGCTC CAGGGCCCCC AGCCCCATCC 
ATCAAAGGCC CTCCGCCAAC CTCTAGTGAC AGCAGCAGCG ATAGCTCATC TGATAGTGAA 
AGCAGTTCAG ACAGTGAGGA GGAACGGGCA CAAAGACTGG CAGAGCTTCA GGAACAGTTG 
AAAGCTGTAC ATGAGCAGTT GGCAGCTCTA TCTCAGCCAC AGCCGAACAA ACCAAAGAAG 
AAAGAGCGTG AAAAGCGGAA GGAAAAGCAC AAACGAAAAG AAGAGGTGGA GGAGCCTAGA 
AAGGGCCGTA TCCGAGAGCC ACCAGCCAAG AAACCCAAGA AAAGTGTTCA AGGGTCCGGA 
GGTACACCAA GCATCAAGAA AGAAGCACCT CCCCCTGTGG CACGCCCTGC TCGTCCAGTG 
CCACCTCCGG CACCATGTGA GTCCTCTGAG GAAGAAACGC AACGATGTCG GCCAATGTCA 
TACGAAGAGA AACGCCAGCT AAGCTTGGAC ATTAATAAGC TGCCAGGCGA AAAACTGGGC 
CGCGTCGTGC ACATCATCCA GTCTCGCGAG CCTTCCCTGA AGAACTCCAA TCCAGATGAG 
ATTGAGATAG ACTTTGAGAC ACTTAAACCG TCCACACTGC GAGAGTTGGA GAGATATGTT 
ACCTCCTGTT TGAGAAAGAA GCGAAAACCA CAGGATAAAA TAGAAGCTCC CACATCTGGC 
ACTGGTAAAG TAAAAGGCTA TTCCTCTTCA GAGTCTGAGA GCTCAAGTGA GTCCAGCACG 
TCAGACAGCG AGGAGTCCGA CCCAGAAACA GCCCCAAATC AGAAGAAAAA GGGGCACTCG 
GGGAGAGAGT CTAGAAAGCA TCATCACCCT ATGCAACAGC CTCTTGCCGC ACCACCCCCT 
GTCATGAAGC CTCCGTCTCC CACCTTAGCA CCACCTTACC CTCCGCCTTC CAGCCTGGAC 
TCCTCACATC CTTCTCTCCA CCACCCTCTC CACTCTGCGA ATGTTTTTGA TGCAGTCATG 
CAGCTTCCTC CTGATCTGCC TCCTCACCTT ACTGGTCAGA CAGAGCACTG CTCGCCCCCA 
CACCTCAACC AACATGCCCT GACAAGCCCT CCAGCCCTAC ACAACGCAAT GCCCCAGCAG 
CCCTCCAGGC CATCCAACCG TGCTGCTGCC CTTCCTCCAA AACCTGTTCA GCCTCCCTCT 
GTCTCTCCTC CTCTTCCTCC TCCTCCTCCG CAGCCCCACC ACCAGCCTTC TGTGCATATC 
CATCATCACC ACCACCATGC ACAGCCCCCT CATGCTCTTC TGGAAGATGA TGGGCCTCCC 
TCTCCTCACA CAGGTCTCCC TTCCTACCTG CAACAGCTTC AGAAGCCACA GCAACCTCCT 
ACTCAGTCTC CTATTCATTC ACTTCTCACA TCTGTCAAGG TGCAGTCCCA AGCACCCATG 
GCTGCTCCTC CCCAATCCAT GAGGCACCTC CAGCCTCTGG TTTACCCTGC TCCATCTTCC 
ACTGCTACCA CAGCGCCTCC TCCTGCCTCT TCTCACATAC ACCAACTGCA GTCTTCCCCT 
GCAGTCCCTC AGCAACAGCC AGCTGGTCAG GCACCGCCAC CCCCCCAGCA GCAGCAGCAC 
CCGGCCCTGC AAGGCGCGCT CGTTTCCTCA CATCCGCAGC ATGTGCAGCA CCAACAGGCC 
AAACAACAAC AAGTGATCCA GCATCATCAT CCTTCCCCAC GGCAGCAGAA GCAAGAGACA 
TATGCTGGAG GTCACTTGCG TGATGCTCCT TCCCCTCTCC TCCTGCATTC GCCTCAGGTT 
CCTCCCTATC CGGGTCTGGC TCACCCACCT TCCCCACAGT CTGTGCAACC TAAAAAGCAG 
GAGATGAGAG GAGCGTCGGT GCTGCAGCCT CAGCCGCTGG TCATGAAAGA AGATAAACGT 
CATTCGCCTT CACTGCGCCC AGAGGGCTTC TCTCCAGGAA TGCGCCCTGA ACCTCAGAAA 
GTTCCGGAGG TTCTAAAAGG ATCTAGTCAC ATCCAGCCAC GACCAGACTT GAAGCCAATG 
GATGTTGGAC GTCCCATACG GCCGACTGAC CAGAGCCTCC CACCTCAAGG GATGCCAGAA 
AAAGAGAAGC AAAAGCAAGA ACCTAAGACA CCTGTGGCTC CTAAAAAGGA CATAAAGATT 
AAAAACATGG GTTCGTGGGC TGGTCTAATG CAGAAGCCAC CAGTAACCCC AACTTCTGCG 
GGAAAGTCAA CAAGTGACAG CTTTGAGCTG TTCCGGAGGC AGGCCCGCGA GAAAGAGGAG 
CGCGAGAGGG CCCTCAAACT TCAGGCCGAA CAAGCAGAGA GAATGCGCAG GGAGCAGGAG 
AGAATGAGGT ACTGCTTGTT TCCTATTGGC TGCTGTGGGG GCAAGAATCC AGAATTTACA 
GAGAGAAGAG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012809169.2
CDS	85..4062
Translation	MRMTSRYVAAALALCEEGAQGANARSPGSACDGSYAMSTETGPGTRLRATSGMGDGVEGAQMSGQQLPAAPQPQPQNSSLLNPPPPDIARPSQAKRQTNQLQYLLKTVLKTLWKHQFAWPFQLPVDVVKLNLPDYHKIIKNPMDMGTIKKRLENHYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKISEMPQEETELAVVQSKGRGRGRKEQSEQDTSITPMRTRVLSGSLGDKSAVKPPPVTPVSKPATPTPPTVTRAPTPPQTRPPQQARPPAITQAPISFSPTISQDVVVPTTVAPTPVPQPLPNHPAVIHTAAQPAKTKKGVKRKADTTTPTTHDPLHESSPLPSDSKPPKAGPRKESGRQIRPTKKTEVPDSQLPAPPVLQPQPTPTAEKDSKTSEQLRYCAGIIREMFSKKHQAYAWPFYKPVDVDALGLHDYREIIKHPMDLGTIKVKMDNWDYKDAKEFASDVRLMFSNCYKYNPPDHEVVIMARKLQDVFEMRYAKMPDEPEEAPAPAPSPAPGPPAPSIKGPPPTSSDSSSDSSSDSESSSDSEEERAQRLAELQEQLKAVHEQLAALSQPQPNKPKKKEREKRKEKHKRKEEVEEPRKGRIREPPAKKPKKSVQGSGGTPSIKKEAPPPVARPARPVPPPAPCESSEEETQRCRPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQDKIEAPTSGTGKVKGYSSSESESSSESSTSDSEESDPETAPNQKKKGHSGRESRKHHHPMQQPLAAPPPVMKPPSPTLAPPYPPPSSLDSSHPSLHHPLHSANVFDAVMQLPPDLPPHLTGQTEHCSPPHLNQHALTSPPALHNAMPQQPSRPSNRAAALPPKPVQPPSVSPPLPPPPPQPHHQPSVHIHHHHHHAQPPHALLEDDGPPSPHTGLPSYLQQLQKPQQPPTQSPIHSLLTSVKVQSQAPMAAPPQSMRHLQPLVYPAPSSTATTAPPPASSHIHQLQSSPAVPQQQPAGQAPPPPQQQQHPALQGALVSSHPQHVQHQQAKQQQVIQHHHPSPRQQKQETYAGGHLRDAPSPLLLHSPQVPPYPGLAHPPSPQSVQPKKQEMRGASVLQPQPLVMKEDKRHSPSLRPEGFSPGMRPEPQKVPEVLKGSSHIQPRPDLKPMDVGRPIRPTDQSLPPQGMPEKEKQKQEPKTPVAPKKDIKIKNMGSWAGLMQKPPVTPTSAGKSTSDSFELFRRQAREKEERERALKLQAEQAERMRREQERMRYCLFPIGCCGGKNPEFTERRED

Target ORF information:

RefSeq Version	XM_012953715.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain containing 4 (brd4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012953715.2

ORF Insert Sequence:

ATGCGCATGA CGTCACGGTA CGTAGCGGCG GCGCTGGCGC TGTGCGAGGA AGGAGCCCAG 
GGAGCGAACG CTCGGTCACC TGGCTCTGCG TGCGATGGGA GCTACGCTAT GTCAACCGAG 
ACTGGGCCTG GTACACGTCT GAGAGCCACA TCTGGAATGG GTGACGGAGT AGAAGGGGCT 
CAGATGTCGG GCCAACAGCT GCCTGCCGCT CCCCAGCCCC AGCCACAGAA CTCATCGCTG 
CTCAATCCTC CTCCTCCTGA TATCGCAAGA CCCAGCCAAG CAAAGCGCCA GACCAACCAG 
CTGCAGTACT TACTTAAGAC AGTGCTCAAG ACATTGTGGA AGCACCAGTT TGCATGGCCT 
TTCCAGCTAC CTGTTGATGT GGTCAAGCTC AATCTGCCTG ATTATCATAA AATAATTAAA 
AACCCAATGG ACATGGGCAC CATTAAAAAG CGCCTCGAGA ACCATTATTA CTGGAACGCC 
CAAGAATGTA TCCAGGACTT CAACACAATG TTTACAAACT GCTACATCTA TAACAAGCCT 
GGAGATGACA TAGTGCTGAT GGCCGAAGCC CTTGAGAAGC TTTTTCTGCA GAAGATCTCA 
GAGATGCCCC AGGAAGAAAC CGAACTGGCA GTTGTGCAGA GCAAAGGCCG TGGCCGGGGA 
AGAAAGGAGC AAAGTGAGCA AGATACTTCA ATCACCCCCA TGAGAACCCG AGTCCTGTCT 
GGGTCACTAG GAGACAAGAG CGCCGTCAAA CCTCCCCCTG TTACACCTGT GTCCAAGCCT 
GCAACCCCAA CGCCCCCTAC TGTGACCAGG GCTCCCACCC CTCCACAAAC CAGACCACCC 
CAACAAGCTC GCCCTCCAGC TATCACTCAA GCCCCCATCA GTTTCTCTCC CACCATCAGT 
CAGGATGTGG TGGTTCCAAC GACCGTAGCC CCAACACCAG TTCCTCAGCC ACTTCCAAAT 
CATCCTGCAG TCATCCACAC TGCTGCCCAG CCTGCAAAGA CAAAGAAAGG TGTGAAGAGG 
AAAGCAGACA CCACAACCCC CACTACCCAT GACCCGCTCC ATGAGTCCTC CCCACTCCCT 
AGTGACTCCA AGCCTCCAAA GGCTGGCCCT CGAAAGGAGA GTGGGCGTCA GATTCGACCA 
ACTAAGAAAA CCGAGGTGCC TGACTCTCAA CTTCCTGCCC CCCCTGTTTT GCAACCCCAA 
CCTACACCCA CTGCTGAGAA GGACAGCAAG ACAAGCGAGC AGTTACGGTA TTGTGCAGGC 
ATTATCCGGG AAATGTTTTC CAAAAAGCAT CAGGCCTATG CGTGGCCATT TTACAAGCCC 
GTTGATGTGG ATGCCCTTGG GTTACATGAC TACCGTGAAA TTATCAAGCA TCCAATGGAC 
CTAGGCACCA TTAAGGTAAA GATGGATAAT TGGGACTACA AGGACGCAAA GGAATTTGCA 
TCTGATGTGC GGCTTATGTT CTCAAACTGT TACAAGTACA ATCCCCCTGA TCATGAAGTT 
GTGATAATGG CTCGCAAGTT GCAGGATGTT TTTGAAATGC GCTATGCCAA AATGCCTGAT 
GAACCTGAGG AGGCGCCAGC TCCTGCACCC TCTCCTGCTC CAGGGCCCCC AGCCCCATCC 
ATCAAAGGCC CTCCGCCAAC CTCTAGTGAC AGCAGCAGCG ATAGCTCATC TGATAGTGAA 
AGCAGTTCAG ACAGTGAGGA GGAACGGGCA CAAAGACTGG CAGAGCTTCA GGAACAGTTG 
AAAGCTGTAC ATGAGCAGTT GGCAGCTCTA TCTCAGCCAC AGCCGAACAA ACCAAAGAAG 
AAAGAGCGTG AAAAGCGGAA GGAAAAGCAC AAACGAAAAG AAGAGGTGGA GGAGCCTAGA 
AAGGGCCGTA TCCGAGAGCC ACCAGCCAAG AAACCCAAGA AAAGTGTTCA AGGGTCCGGA 
GGTACACCAA GCATCAAGAA AGAAGCACCT CCCCCTGTGG CACGCCCTGC TCGTCCAGTG 
CCACCTCCGG CACCATGTGA GTCCTCTGAG GAAGAAACGC AACGATGTCG GCCAATGTCA 
TACGAAGAGA AACGCCAGCT AAGCTTGGAC ATTAATAAGC TGCCAGGCGA AAAACTGGGC 
CGCGTCGTGC ACATCATCCA GTCTCGCGAG CCTTCCCTGA AGAACTCCAA TCCAGATGAG 
ATTGAGATAG ACTTTGAGAC ACTTAAACCG TCCACACTGC GAGAGTTGGA GAGATATGTT 
ACCTCCTGTT TGAGAAAGAA GCGAAAACCA CAGGATAAAA TAGAAGCTCC CACATCTGGC 
ACTGGTAAAG TAAAAGGCTA TTCCTCTTCA GAGTCTGAGA GCTCAAGTGA GTCCAGCACG 
TCAGACAGCG AGGAGTCCGA CCCAGAAACA GCCCCAAATC AGAAGAAAAA GGGGCACTCG 
GGGAGAGAGT CTAGAAAGCA TCATCACCCT ATGCAACAGC CTCTTGCCGC ACCACCCCCT 
GTCATGAAGC CTCCGTCTCC CACCTTAGCA CCACCTTACC CTCCGCCTTC CAGCCTGGAC 
TCCTCACATC CTTCTCTCCA CCACCCTCTC CACTCTGCGA ATGTTTTTGA TGCAGTCATG 
CAGCTTCCTC CTGATCTGCC TCCTCACCTT ACTGGTCAGA CAGAGCACTG CTCGCCCCCA 
CACCTCAACC AACATGCCCT GACAAGCCCT CCAGCCCTAC ACAACGCAAT GCCCCAGCAG 
CCCTCCAGGC CATCCAACCG TGCTGCTGCC CTTCCTCCAA AACCTGTTCA GCCTCCCTCT 
GTCTCTCCTC CTCTTCCTCC TCCTCCTCCG CAGCCCCACC ACCAGCCTTC TGTGCATATC 
CATCATCACC ACCACCATGC ACAGCCCCCT CATGCTCTTC TGGAAGATGA TGGGCCTCCC 
TCTCCTCACA CAGGTCTCCC TTCCTACCTG CAACAGCTTC AGAAGCCACA GCAACCTCCT 
ACTCAGTCTC CTATTCATTC ACTTCTCACA TCTGTCAAGG TGCAGTCCCA AGCACCCATG 
GCTGCTCCTC CCCAATCCAT GAGGCACCTC CAGCCTCTGG TTTACCCTGC TCCATCTTCC 
ACTGCTACCA CAGCGCCTCC TCCTGCCTCT TCTCACATAC ACCAACTGCA GTCTTCCCCT 
GCAGTCCCTC AGCAACAGCC AGCTGGTCAG GCACCGCCAC CCCCCCAGCA GCAGCAGCAC 
CCGGCCCTGC AAGGCGCGCT CGTTTCCTCA CATCCGCAGC ATGTGCAGCA CCAACAGGCC 
AAACAACAAC AAGTGATCCA GCATCATCAT CCTTCCCCAC GGCAGCAGAA GCAAGAGACA 
TATGCTGGAG GTCACTTGCG TGATGCTCCT TCCCCTCTCC TCCTGCATTC GCCTCAGGTT 
CCTCCCTATC CGGGTCTGGC TCACCCACCT TCCCCACAGT CTGTGCAACC TAAAAAGCAG 
GAGATGAGAG GAGCGTCGGT GCTGCAGCCT CAGCCGCTGG TCATGAAAGA AGATAAACGT 
CATTCGCCTT CACTGCGCCC AGAGGGCTTC TCTCCAGGAA TGCGCCCTGA ACCTCAGAAA 
GTTCCGGAGG TTCTAAAAGG ATCTAGTCAC ATCCAGCCAC GACCAGACTT GAAGCCAATG 
GATGTTGGAC GTCCCATACG GCCGACTGAC CAGAGCCTCC CACCTCAAGG GATGCCAGAA 
AAAGAGAAGC AAAAGCAAGA ACCTAAGACA CCTGTGGCTC CTAAAAAGGA CATAAAGATT 
AAAAACATGG GTTCGTGGGC TGGTCTAATG CAGAAGCCAC CAGTAACCCC AACTTCTGCG 
GGAAAGTCAA CAAGTGACAG CTTTGAGCTG TTCCGGAGGC AGGCCCGCGA GAAAGAGGAG 
CGCGAGAGGG CCCTCAAACT TCAGGCCGAA CAAGCAGAGA GAATGCGCAG GGAGCAGGAG 
AGAATGAGGT ACTGCTTGTT TCCTATTGGC TGCTGTGGGG GCAAGAATCC AGAATTTACA 
GAGAGAAGAG AGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

brd4 ( NM_001142218.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001135690.1 Xenopus tropicalis bromodomain containing 4 (brd4), mRNA.

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CloneID	OXa23651
Clone ID Related Accession (Same CDS sequence)	NM_001142218.1
Accession Version	NM_001142218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	bromodomain-containing protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168637.1. ##Evidence-Data-START## Transcript exon combination :: BC168637.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

ATGTCAACCG AGACTGGGCC TGGTACACGT CTGAGAGCCA CATCTGGAAT GGGTGACGGA 
GTAGAAGGGG CTCAGATGTC GGGCCAACAG CTGCCTGCCG CTCCCCAGCC CCAGCCACAG 
AACTCATCGC TGCTCAATCC TCCTCCTCCT GATATCGCAA GACCCAGCCA AGCAAAGCGC 
CAGACCAACC AGCTGCAGTA CTTACTTAAG ACAGTGCTCA AGACATTGTG GAAGCACCAG 
TTTGCATGGC CTTTCCAGCT ACCTGTTGAT GTGGTCAAGC TCAATCTGCC TGATTATCAT 
AAAATAATTA AAAACCCAAT GGACATGGGC ACCATTAAAA AGCGCCTCGA GAACCATTAT 
TACTGGAACG CCCAAGAATG TATCCAGGAC TTCAACACAA TGTTTACAAA CTGCTACATC 
TATAACAAGC CTGGAGATGA CATAGTGCTG ATGGCCGAAG CCCTTGAGAA GCTTTTTCTG 
CAGAAGATCT CAGAGATGCC CCAGGAAGAA ACCGAACTGG CAGTTGTGCA GAGCAAAGGC 
CGTGGCCGGG GAAGAAAGGA GCAAAGTGAG CAAGATACTT CAATCACCCC CATGAGAACC 
CGAGTCCTGT CTGGGTCACT AGGAGACAAG AGCGCCGTCA AACCTCCCCC TGTTACACCT 
GTGTCCAAGC CTGCAACCCC AACGCCCCCT ACTGTGACCA GGGCTCCCAC CCCTCCACAA 
ACCAGACCAC CCCAACAAGC TCGCCCTCCA GCTATCACTC AAGCCCCCAT CAGTTTCTCT 
CCCACCATCA GTCAGGATGT GGTGGTTCCA ACGACCGTAG CCCCAACACC AGTTCCTCAG 
CCACTTCCAA ATCATCCTGC AGTCATCCAC ACTGCTGCCC AGCCTGCAAA GACAAAGAAA 
GGTGTGAAGA GGAAAGCAGA CACCACAACC CCCACTACCC ATGACCCGCT CCATGAGTCC 
TCCCCACTCC CTAGTGACTC CAAGCCTCCA AAGGCTGGCC CTCGAAAGGA GAGTGGGCGT 
CAGATTCGAC CAACTAAGAA AACCGAGGTG CCTGACTCTC AACTTCCTGC CCCCCCTGTT 
TTGCAACCCC AACCTACACC CACTGCTGAG AAGGACAGCA AGACAAGCGA GCAGTTACGG 
TATTGTGCAG GCATTATCCG GGAAATGTTT TCCAAAAAGC ATCAGGCCTA TGCGTGGCCA 
TTTTACAAGC CCGTTGATGT GGATGCCCTT GGGTTACATG ACTACCGTGA AATTATCAAG 
CATCCAATGG ACCTAGGCAC CATTAAGGTA AAGATGGATA ATTGGGACTA CAAGGACGCA 
AAGGAATTTG CATCTGATGT GCGGCTTATG TTCTCAAACT GTTACAAGTA CAATCCCCCT 
GATCATGAAG TTGTGATAAT GGCTCGCAAG TTGCAGGATG TTTTTGAAAT GCGCTATGCC 
AAAATGCCTG ATGAACCCGA GGAGGCGCCA GCTCCTGCAC CCTCTCCTGC TCCAGGGCCC 
CCAGCCCCAT CCATCAAAGG CCCTCCGCCA ACCTCTAGTG ACAGCAGCAG CGATAGCTCA 
TCTGATAGTG AAAGCAGTTC AGACAGTGAG GAGGAACGGG CACAAAGACT GGCAGAGCTT 
CAGGAACAGT TGAAAGCTGT ACATGAGCAG TTGGCAGCTC TATCTCAGCC ACAGCCGAAC 
AAACCAAAGA AGAAAGAGCG TGAAAAGCGG AAGGAAAAGC ACAAACGAAA AGAAGAGGTG 
GAGGAGCCTA GAAAGGGCCG TATCCGAGAG CCACCAGCCA AGAAACCCAA GAAAAGTGTT 
CAAGGGTCCG GAGGTACACC AAGCATCAAG AAAGAAGCAC CTCCCCCTGT GGCACGCCCT 
GCTCGTCCAG TGCCACCTCC GGCACCATGT GAGTCCTCTG AGGAAGAAAC GCAACGATGT 
CGGCCAATGT CATACGAAGA GAAACGCCAG CTAAGCTTGG ACATTAATAA GCTGCCAGGC 
GAAAAACTGG GCCGCGTCGT GCACATCATC CAGTCTCGCG AGCCTTCCCT GAAGAACTCC 
AATCCAGATG AGATTGAGAT AGACTTTGAG ACACTTAAAC CGTCCACACT GCGAGAGTTG 
GAGAGATATG TTACCTCCTG TTTGAGAAAG AAGCGAAAAC CACAGGATAA AATAGAAGCT 
CCCACATCTG GCACTGGTAA AGTAAAAGGC TATTCCTCTT CAGAGTCTGA GAGCTCAAGT 
GAGTCCAGCA CGTCAGACAG CGAGGAGTCC GACCCAGAAA CAGCCCCAAA TCAGAAGAAA 
AAGGGGCACT CGGGGAGAGA GTCTAGAAAG CATCATCACC CTATGCAACA GCCTCTTGCC 
GCACCACCCC CTGTCATGAA GCCTCCGTCT CCCACCTTAG CACCACCTTA CCCTCCGCCT 
TCCAGCCTGG ACTCCTCACA TCCTTCTCTC CACCACCCTC TCCACTCTGC GAATGTTTTT 
GATGCAGTCA TGCAGCTTCC TCCTGATCTG CCTCCTCACC TTACTGGTCA GACAGAGCAC 
TGCTCGCCCC CACACCTCAA CCAACATGCC CTGACAAGCC CTCCAGCCCT ACACAACGCA 
ATGCCCCAGC AGCCCTCCCG GCCATCCAAC CGTGCTGCTG CCCTTCCTCC AAAACCTGTT 
CAGCCTCCCT CTGTCTCTCC TCCTCTTCCT CCTCCTCCTC CGCAGCCCCA CCACCAGCCT 
TCTGTGCATA TCCATCATCA CCACCACCAT GCACAGCCCC CTCATGCTCT TCTGGAAGAT 
GATGGGCCTC CCTCTCCTCA CACAGGTCTC CCTTCCTACC TGCAACAGCT TCAGAAGCCA 
CAGCAACCTC CTACTCAGTC ACCTATTCAT TCACTTCTCA CATCTGTCAA GGTGCAGTCC 
CAAGCACCCA TGGCTGCTCC TCCCCAATCC ATGAGGCACC TCCAGCCTCT GGTTTACCCT 
GCTCCATCTT CCACTGCTAC CACAGCGCCT CCTCCTGCCT CTTCTCACAT ACACCAACTG 
CAGTCTTCCC CTGCAGTCCC TCAGCAACAG CCAGCTGGTC AGGCACCGCC ACCCCCCCAG 
CAGCAGCAGC ACCCGGCCCT GCAAGGCACG CTCGTTTCCT CACATCCGCA GCATGTGCAG 
CACCAACAGG CCAAACAACA ACAAGTGATC CAGCATCATC ATCCTTCCCC ACGGCAGCAG 
AAGCAAGAGA CATATGCTGG AGGTCACTTG CGTGATGCTC CTTCCCCTCT CCTCCTGCAT 
TCGCCTCAGG TTCCTCCCTA TCCGGGTCTG GCTCACCCAC CTTCCCCACA GTCTGTGCAA 
CCTAAAAAGC AGGAGATGAG AGGAGCGTCG GTGCTGCAGC CTCAGCCGCT GGTCATGAAA 
GAAGATAAAC GTCATTCGCC TTCACTGCGC CCAGAGGGCT TCTCTCCAGG AATGCGCCCT 
GAACCTCAGA AAGTTCCGGA GGTTCTAAAA GGATCTAGTC ACATCCAGCC ACGACCAGAC 
TTGAAGCCAA TGGATGTTGG GCGTCCCATA CGGCCGACTG ACCAGAGCCT CCCACCTCAA 
GGGATGCCAG AAAAAGAGAA GCAAAAGCAA GAACCTAAGA CACCTGTGGC TCCTAAAAAG 
GACATAAAGA TTAAAAACAT GGGTTCGTGG GCTGGTCTAA TGCAGAAGCC ACCAGTGACC 
CCAACTTCTG CGGGAAAGTC AACAAGTGAC AGCTTTGAGC TGTTCCGGAG GCAGGCCCGC 
GAGAAAGAGG AGCGCGAGAG GGCCCTCAAA CTTCAGGCCG AACAAGCAGA GAGAATGCGC 
AGGGAGCAGG AGAGAATGAG GTCCCGGGAT GAAGATGACG CGCAGGAGCA GGCTAGGAAA 
GCCCACGAGG AGGCACGCCG GCGGCAGGAA CAACAGCAAC AGCAGCAACA ACAACAGCAG 
CAACAGCAAC ACGTGCAGAG CAACCTCCCC GCCGCTCCGT CACCTGCGCA GAGCTCTCAG 
CCAATCATGG ATCAGCGAGA AATGGCTAGA AAGCGGGAAC AGGAGAGGAG ACGAAGGCAG 
GCGATGGCGC CCTCTATAGA TATGAATTTT CAGAGTGAAC TGATGGAGAT ATTTGAACAG 
AACCTGTTCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001135690.1
CDS	190..4284
Translation	MSTETGPGTRLRATSGMGDGVEGAQMSGQQLPAAPQPQPQNSSLLNPPPPDIARPSQAKRQTNQLQYLLKTVLKTLWKHQFAWPFQLPVDVVKLNLPDYHKIIKNPMDMGTIKKRLENHYYWNAQECIQDFNTMFTNCYIYNKPGDDIVLMAEALEKLFLQKISEMPQEETELAVVQSKGRGRGRKEQSEQDTSITPMRTRVLSGSLGDKSAVKPPPVTPVSKPATPTPPTVTRAPTPPQTRPPQQARPPAITQAPISFSPTISQDVVVPTTVAPTPVPQPLPNHPAVIHTAAQPAKTKKGVKRKADTTTPTTHDPLHESSPLPSDSKPPKAGPRKESGRQIRPTKKTEVPDSQLPAPPVLQPQPTPTAEKDSKTSEQLRYCAGIIREMFSKKHQAYAWPFYKPVDVDALGLHDYREIIKHPMDLGTIKVKMDNWDYKDAKEFASDVRLMFSNCYKYNPPDHEVVIMARKLQDVFEMRYAKMPDEPEEAPAPAPSPAPGPPAPSIKGPPPTSSDSSSDSSSDSESSSDSEEERAQRLAELQEQLKAVHEQLAALSQPQPNKPKKKEREKRKEKHKRKEEVEEPRKGRIREPPAKKPKKSVQGSGGTPSIKKEAPPPVARPARPVPPPAPCESSEEETQRCRPMSYEEKRQLSLDINKLPGEKLGRVVHIIQSREPSLKNSNPDEIEIDFETLKPSTLRELERYVTSCLRKKRKPQDKIEAPTSGTGKVKGYSSSESESSSESSTSDSEESDPETAPNQKKKGHSGRESRKHHHPMQQPLAAPPPVMKPPSPTLAPPYPPPSSLDSSHPSLHHPLHSANVFDAVMQLPPDLPPHLTGQTEHCSPPHLNQHALTSPPALHNAMPQQPSRPSNRAAALPPKPVQPPSVSPPLPPPPPQPHHQPSVHIHHHHHHAQPPHALLEDDGPPSPHTGLPSYLQQLQKPQQPPTQSPIHSLLTSVKVQSQAPMAAPPQSMRHLQPLVYPAPSSTATTAPPPASSHIHQLQSSPAVPQQQPAGQAPPPPQQQQHPALQGTLVSSHPQHVQHQQAKQQQVIQHHHPSPRQQKQETYAGGHLRDAPSPLLLHSPQVPPYPGLAHPPSPQSVQPKKQEMRGASVLQPQPLVMKEDKRHSPSLRPEGFSPGMRPEPQKVPEVLKGSSHIQPRPDLKPMDVGRPIRPTDQSLPPQGMPEKEKQKQEPKTPVAPKKDIKIKNMGSWAGLMQKPPVTPTSAGKSTSDSFELFRRQAREKEERERALKLQAEQAERMRREQERMRSRDEDDAQEQARKAHEEARRRQEQQQQQQQQQQQQQHVQSNLPAAPSPAQSSQPIMDQREMARKREQERRRRQAMAPSIDMNFQSELMEIFEQNLFS

Target ORF information:

RefSeq Version	NM_001142218.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis bromodomain containing 4 (brd4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142218.1

ORF Insert Sequence:

ATGTCAACCG AGACTGGGCC TGGTACACGT CTGAGAGCCA CATCTGGAAT GGGTGACGGA 
GTAGAAGGGG CTCAGATGTC GGGCCAACAG CTGCCTGCCG CTCCCCAGCC CCAGCCACAG 
AACTCATCGC TGCTCAATCC TCCTCCTCCT GATATCGCAA GACCCAGCCA AGCAAAGCGC 
CAGACCAACC AGCTGCAGTA CTTACTTAAG ACAGTGCTCA AGACATTGTG GAAGCACCAG 
TTTGCATGGC CTTTCCAGCT ACCTGTTGAT GTGGTCAAGC TCAATCTGCC TGATTATCAT 
AAAATAATTA AAAACCCAAT GGACATGGGC ACCATTAAAA AGCGCCTCGA GAACCATTAT 
TACTGGAACG CCCAAGAATG TATCCAGGAC TTCAACACAA TGTTTACAAA CTGCTACATC 
TATAACAAGC CTGGAGATGA CATAGTGCTG ATGGCCGAAG CCCTTGAGAA GCTTTTTCTG 
CAGAAGATCT CAGAGATGCC CCAGGAAGAA ACCGAACTGG CAGTTGTGCA GAGCAAAGGC 
CGTGGCCGGG GAAGAAAGGA GCAAAGTGAG CAAGATACTT CAATCACCCC CATGAGAACC 
CGAGTCCTGT CTGGGTCACT AGGAGACAAG AGCGCCGTCA AACCTCCCCC TGTTACACCT 
GTGTCCAAGC CTGCAACCCC AACGCCCCCT ACTGTGACCA GGGCTCCCAC CCCTCCACAA 
ACCAGACCAC CCCAACAAGC TCGCCCTCCA GCTATCACTC AAGCCCCCAT CAGTTTCTCT 
CCCACCATCA GTCAGGATGT GGTGGTTCCA ACGACCGTAG CCCCAACACC AGTTCCTCAG 
CCACTTCCAA ATCATCCTGC AGTCATCCAC ACTGCTGCCC AGCCTGCAAA GACAAAGAAA 
GGTGTGAAGA GGAAAGCAGA CACCACAACC CCCACTACCC ATGACCCGCT CCATGAGTCC 
TCCCCACTCC CTAGTGACTC CAAGCCTCCA AAGGCTGGCC CTCGAAAGGA GAGTGGGCGT 
CAGATTCGAC CAACTAAGAA AACCGAGGTG CCTGACTCTC AACTTCCTGC CCCCCCTGTT 
TTGCAACCCC AACCTACACC CACTGCTGAG AAGGACAGCA AGACAAGCGA GCAGTTACGG 
TATTGTGCAG GCATTATCCG GGAAATGTTT TCCAAAAAGC ATCAGGCCTA TGCGTGGCCA 
TTTTACAAGC CCGTTGATGT GGATGCCCTT GGGTTACATG ACTACCGTGA AATTATCAAG 
CATCCAATGG ACCTAGGCAC CATTAAGGTA AAGATGGATA ATTGGGACTA CAAGGACGCA 
AAGGAATTTG CATCTGATGT GCGGCTTATG TTCTCAAACT GTTACAAGTA CAATCCCCCT 
GATCATGAAG TTGTGATAAT GGCTCGCAAG TTGCAGGATG TTTTTGAAAT GCGCTATGCC 
AAAATGCCTG ATGAACCCGA GGAGGCGCCA GCTCCTGCAC CCTCTCCTGC TCCAGGGCCC 
CCAGCCCCAT CCATCAAAGG CCCTCCGCCA ACCTCTAGTG ACAGCAGCAG CGATAGCTCA 
TCTGATAGTG AAAGCAGTTC AGACAGTGAG GAGGAACGGG CACAAAGACT GGCAGAGCTT 
CAGGAACAGT TGAAAGCTGT ACATGAGCAG TTGGCAGCTC TATCTCAGCC ACAGCCGAAC 
AAACCAAAGA AGAAAGAGCG TGAAAAGCGG AAGGAAAAGC ACAAACGAAA AGAAGAGGTG 
GAGGAGCCTA GAAAGGGCCG TATCCGAGAG CCACCAGCCA AGAAACCCAA GAAAAGTGTT 
CAAGGGTCCG GAGGTACACC AAGCATCAAG AAAGAAGCAC CTCCCCCTGT GGCACGCCCT 
GCTCGTCCAG TGCCACCTCC GGCACCATGT GAGTCCTCTG AGGAAGAAAC GCAACGATGT 
CGGCCAATGT CATACGAAGA GAAACGCCAG CTAAGCTTGG ACATTAATAA GCTGCCAGGC 
GAAAAACTGG GCCGCGTCGT GCACATCATC CAGTCTCGCG AGCCTTCCCT GAAGAACTCC 
AATCCAGATG AGATTGAGAT AGACTTTGAG ACACTTAAAC CGTCCACACT GCGAGAGTTG 
GAGAGATATG TTACCTCCTG TTTGAGAAAG AAGCGAAAAC CACAGGATAA AATAGAAGCT 
CCCACATCTG GCACTGGTAA AGTAAAAGGC TATTCCTCTT CAGAGTCTGA GAGCTCAAGT 
GAGTCCAGCA CGTCAGACAG CGAGGAGTCC GACCCAGAAA CAGCCCCAAA TCAGAAGAAA 
AAGGGGCACT CGGGGAGAGA GTCTAGAAAG CATCATCACC CTATGCAACA GCCTCTTGCC 
GCACCACCCC CTGTCATGAA GCCTCCGTCT CCCACCTTAG CACCACCTTA CCCTCCGCCT 
TCCAGCCTGG ACTCCTCACA TCCTTCTCTC CACCACCCTC TCCACTCTGC GAATGTTTTT 
GATGCAGTCA TGCAGCTTCC TCCTGATCTG CCTCCTCACC TTACTGGTCA GACAGAGCAC 
TGCTCGCCCC CACACCTCAA CCAACATGCC CTGACAAGCC CTCCAGCCCT ACACAACGCA 
ATGCCCCAGC AGCCCTCCCG GCCATCCAAC CGTGCTGCTG CCCTTCCTCC AAAACCTGTT 
CAGCCTCCCT CTGTCTCTCC TCCTCTTCCT CCTCCTCCTC CGCAGCCCCA CCACCAGCCT 
TCTGTGCATA TCCATCATCA CCACCACCAT GCACAGCCCC CTCATGCTCT TCTGGAAGAT 
GATGGGCCTC CCTCTCCTCA CACAGGTCTC CCTTCCTACC TGCAACAGCT TCAGAAGCCA 
CAGCAACCTC CTACTCAGTC ACCTATTCAT TCACTTCTCA CATCTGTCAA GGTGCAGTCC 
CAAGCACCCA TGGCTGCTCC TCCCCAATCC ATGAGGCACC TCCAGCCTCT GGTTTACCCT 
GCTCCATCTT CCACTGCTAC CACAGCGCCT CCTCCTGCCT CTTCTCACAT ACACCAACTG 
CAGTCTTCCC CTGCAGTCCC TCAGCAACAG CCAGCTGGTC AGGCACCGCC ACCCCCCCAG 
CAGCAGCAGC ACCCGGCCCT GCAAGGCACG CTCGTTTCCT CACATCCGCA GCATGTGCAG 
CACCAACAGG CCAAACAACA ACAAGTGATC CAGCATCATC ATCCTTCCCC ACGGCAGCAG 
AAGCAAGAGA CATATGCTGG AGGTCACTTG CGTGATGCTC CTTCCCCTCT CCTCCTGCAT 
TCGCCTCAGG TTCCTCCCTA TCCGGGTCTG GCTCACCCAC CTTCCCCACA GTCTGTGCAA 
CCTAAAAAGC AGGAGATGAG AGGAGCGTCG GTGCTGCAGC CTCAGCCGCT GGTCATGAAA 
GAAGATAAAC GTCATTCGCC TTCACTGCGC CCAGAGGGCT TCTCTCCAGG AATGCGCCCT 
GAACCTCAGA AAGTTCCGGA GGTTCTAAAA GGATCTAGTC ACATCCAGCC ACGACCAGAC 
TTGAAGCCAA TGGATGTTGG GCGTCCCATA CGGCCGACTG ACCAGAGCCT CCCACCTCAA 
GGGATGCCAG AAAAAGAGAA GCAAAAGCAA GAACCTAAGA CACCTGTGGC TCCTAAAAAG 
GACATAAAGA TTAAAAACAT GGGTTCGTGG GCTGGTCTAA TGCAGAAGCC ACCAGTGACC 
CCAACTTCTG CGGGAAAGTC AACAAGTGAC AGCTTTGAGC TGTTCCGGAG GCAGGCCCGC 
GAGAAAGAGG AGCGCGAGAG GGCCCTCAAA CTTCAGGCCG AACAAGCAGA GAGAATGCGC 
AGGGAGCAGG AGAGAATGAG GTCCCGGGAT GAAGATGACG CGCAGGAGCA GGCTAGGAAA 
GCCCACGAGG AGGCACGCCG GCGGCAGGAA CAACAGCAAC AGCAGCAACA ACAACAGCAG 
CAACAGCAAC ACGTGCAGAG CAACCTCCCC GCCGCTCCGT CACCTGCGCA GAGCTCTCAG 
CCAATCATGG ATCAGCGAGA AATGGCTAGA AAGCGGGAAC AGGAGAGGAG ACGAAGGCAG 
GCGATGGCGC CCTCTATAGA TATGAATTTT CAGAGTGAAC TGATGGAGAT ATTTGAACAG 
AACCTGTTCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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brd4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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